LOCUS       DAXHKX010000001        75989 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHKX010000001 DAXHKX010000000
VERSION     DAXHKX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 75989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.001"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(18..1271)
                     /locus_tag="ACFMWZ_000001"
     CDS             complement(18..1271)
                     /locus_tag="ACFMWZ_000001"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC5934034.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVEKLRSKTIIDVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            complement(1343..2971)
                     /locus_tag="ACFMWZ_000002"
     CDS             complement(1343..2971)
                     /locus_tag="ACFMWZ_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC5934035.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGTIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            3121..3495
                     /locus_tag="ACFMWZ_000003"
     CDS             3121..3495
                     /locus_tag="ACFMWZ_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC5934036.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            3555..5093
                     /locus_tag="ACFMWZ_000004"
     CDS             3555..5093
                     /locus_tag="ACFMWZ_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC5934037.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVTDFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            5160..5777
                     /gene="recR"
                     /locus_tag="ACFMWZ_000005"
     CDS             5160..5777
                     /gene="recR"
                     /locus_tag="ACFMWZ_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC5934038.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVDHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5833..6111)
                     /locus_tag="ACFMWZ_000006"
     CDS             complement(5833..6111)
                     /locus_tag="ACFMWZ_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934039.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAV
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            6316..7563
                     /locus_tag="ACFMWZ_000007"
     CDS             6316..7563
                     /locus_tag="ACFMWZ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC5934040.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            7556..8251
                     /gene="lolD"
                     /locus_tag="ACFMWZ_000008"
     CDS             7556..8251
                     /gene="lolD"
                     /locus_tag="ACFMWZ_000008"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC5934041.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKNREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGSLHPKQGANA"
     gene            8319..10064
                     /gene="recD"
                     /locus_tag="ACFMWZ_000009"
     CDS             8319..10064
                     /gene="recD"
                     /locus_tag="ACFMWZ_000009"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC5934042.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSSDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETYKSLNLYLPEHGFPVHEDAPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGGLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSRGLLYTAITRAREKFVFFGGEEAFRQAASTVKAR
                     QTALGSMLGRIFSQG"
     gene            complement(10092..11195)
                     /locus_tag="ACFMWZ_000010"
     CDS             complement(10092..11195)
                     /locus_tag="ACFMWZ_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC5934043.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRSALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(11170..11346)
                     /locus_tag="ACFMWZ_000011"
     CDS             complement(11170..11346)
                     /locus_tag="ACFMWZ_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HFC5934044.1"
                     /translation="MPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVLPPYFQ
                     NMTQVFHVFFYFSR"
     gene            11446..13908
                     /gene="lon"
                     /locus_tag="ACFMWZ_000012"
     CDS             11446..13908
                     /gene="lon"
                     /locus_tag="ACFMWZ_000012"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC5934045.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVCADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            14090..14359
                     /locus_tag="ACFMWZ_000013"
     CDS             14090..14359
                     /locus_tag="ACFMWZ_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC5934046.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(14418..14852)
                     /locus_tag="ACFMWZ_000014"
     CDS             complement(14418..14852)
                     /locus_tag="ACFMWZ_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934047.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVTDLKSE"
     gene            complement(14845..16152)
                     /locus_tag="ACFMWZ_000015"
     CDS             complement(14845..16152)
                     /locus_tag="ACFMWZ_000015"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC5934048.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            16349..16720
                     /locus_tag="ACFMWZ_000016"
     CDS             16349..16720
                     /locus_tag="ACFMWZ_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC5934049.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLARQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIGVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            16797..18719
                     /locus_tag="ACFMWZ_000017"
     CDS             16797..18719
                     /locus_tag="ACFMWZ_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC5934050.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLTKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            18682..19119
                     /locus_tag="ACFMWZ_000018"
     CDS             18682..19119
                     /locus_tag="ACFMWZ_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC5934051.1"
                     /translation="MKLKSRNSLPSDKEARTGCLSLPANNGIIRALSPPFTGINIRLF
                     AALGLLSLSGAAAQASVYHCNSNGKSVYTSAPAGSCADADLPKISSHQGGGYRLKIKK
                     LSQEAKIHIESKKKNKKPAGKKNRQAAKAPKENQKQTTEKKSP"
     gene            19185..19700
                     /locus_tag="ACFMWZ_000019"
     CDS             19185..19700
                     /locus_tag="ACFMWZ_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934052.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            19733..19990
                     /locus_tag="ACFMWZ_000020"
                     /pseudo
     CDS             19733..19990
                     /locus_tag="ACFMWZ_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19894..21714)
                     /gene="traD"
                     /locus_tag="ACFMWZ_000021"
     CDS             complement(19894..21714)
                     /gene="traD"
                     /locus_tag="ACFMWZ_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFC5934053.1"
                     /translation="MSAHFPENVYRSPYEWISVAAYSTTAVSVAHLPVPPTIAYGSLA
                     IFSLVSVKRALSALRIMRFRKNIRKLPYYALTADEIPSSDKALFLGKGFRWTQTHTQR
                     LFMVRQPENDGLRNPSKLYKGARKHEIETMGTDWISRITRKSAWWNPVAPLPPVGGSP
                     ELHGIEIEEDDIWEDLRERVGHKVVLGTTRVGKTRLCETMIVQDIRRGDVTIAFDPKG
                     DADLMTRMYVESQKSNRPFYMFHLGFPEYSCRYNPIGDYGRITEVATRIANQLPSEGQ
                     SAAFRDFVWRFVNVLTKTMEGLSIKPSYENIYKSAANVDELAGDYFKKILEKHHPGWE
                     KDFDNFDMPESEAKSAVKTGRNLDTMKLGSFLKAKKHNEPLIDALGGILSNDRSYFEK
                     LVSSLYPLLEKLTTGEVAKLISPDTEDLSDPRIIMDWRKVMESNAVVYIGLDSLSDAE
                     VAAAVGNAMFADLTSLAGQIYKFGHGVGQSGKTQKRKVSIHADEFNELIGDEFIPLLN
                     KAGGAGYQVTVYTQTWKDVEAKIGSPAKAGQIGGNLNTMIMLRVKTVETAEMLTNQLP
                     EVKIMTSTLYTLCELSETVVRVPVVFPSRKIKVWTMRLNV"
     gene            complement(21868..24171)
                     /gene="mobH"
                     /locus_tag="ACFMWZ_000022"
     CDS             complement(21868..24171)
                     /gene="mobH"
                     /locus_tag="ACFMWZ_000022"
                     /inference="COORDINATES: protein motif:HMM:NF041494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFC5934054.1"
                     /translation="MSHNDEVAHINQNIVNDRFRILNAHELIQVLDLSPQISGIKMNL
                     GLSDENWSKDALPFLEKYIAFVQRLPASESHHHAGDGGLVRHTLDVAALALVASTSQS
                     WPPNAKTEEIAKKTAVWRYGIMCAALLHDVGKTVTGFQVELFDSAISLEKLLWLPDTG
                     SMAESGKLYYRVEFPDAKSAYSTHAEIAWTFFQALVPSHVRQWLATTDPNLMITLRNY
                     LSGKKDGSPLEQLIKNADMTSVSRDLRSGSRQRFSTAKRKPFIETIMETLKEMLSDRG
                     VHFSIATTAGGDLFRKSDRIYLMSKNVPDYIRQYLRKNQHPAAGSFPADNQRIFDTLF
                     EYRAVIPPENDPHRAINHIEVEFTRMDGEKIRNIFSVLCFNAKTLYPDGDYPTEFLGN
                     LSEVSAKKEIPDVVVSEDKVSTQTQDAEDSSVTMTKDKMDFLSIQPVIAEQPPLPEKN
                     QTETANTAFDNSKNTAAPAKNKSSESEKAKELGYSTIDNLIENFNLIEADSEQTESTN
                     SEAETAENAVNLVQENIRVEVKTETEDKPRAEVKETETDNSPKTIKSGTKASRKKLAD
                     LFADNRKPIGKTAEPEPECAQIKTTETVTHQIDSEAVPLPESESAQNGMRELEQLRLR
                     EPTSSPRPVQVMDDSEGLDSLASGAKNMAELEAIVSERNLKKQSETESTEIVEDSVKA
                     KHMEARDRGMQFLRWLADGLGDGSIAVNRSGATVHFIEQGMLLVTPAVFRDYAGGVFN
                     KSDTESLGPLTQKGFEVACKNEKKLIA"
     gene            complement(24261..24563)
                     /locus_tag="ACFMWZ_000023"
     CDS             complement(24261..24563)
                     /locus_tag="ACFMWZ_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934055.1"
                     /translation="MMTLDDDENTATFLIEKLEKLLEPLPIDIDNTTFAEALADDFEI
                     LFLMAQYGSEYWNSLEESFEEFCIRHTTQPNQLIADIPHAKKEKVTMWIKSLLKLS"
     gene            25188..25649
                     /locus_tag="ACFMWZ_000024"
     CDS             25188..25649
                     /locus_tag="ACFMWZ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC5934056.1"
                     /translation="MRLRAILLQFVVPGVLVLTSTRAVAFCYKEAGSKYGIDPAMLQS
                     IALTESAFRPNIESHTADIGLMGINRSWLPVLHKKFGLTEADVWNPCTNVHIGAWILA
                     NSYRQYGKSWEAVGAYNAACTKLKGRACYRARMTYANKVYQNWKRLKTRSS"
     gene            25619..26140
                     /locus_tag="ACFMWZ_000025"
     CDS             25619..26140
                     /locus_tag="ACFMWZ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC5934057.1"
                     /translation="METIKNTLFVASTMILTACAAPKQEVAELPQDATYTVEQNKLGE
                     FVFCRNEECSGFERKVLAEQPVYAQTQTNLQKEEIRVFFNLGSSHLGAEGVKTLKRVL
                     PKLKNTQTVYLRGWADSIGGRNTAINRRLAKQRAAVIGKWLRKHNVTAKIRTASSPAC
                     CNSDDTRTVVITW"
     gene            26195..26491
                     /gene="traA"
                     /locus_tag="ACFMWZ_000026"
     CDS             26195..26491
                     /gene="traA"
                     /locus_tag="ACFMWZ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HFC5934058.1"
                     /translation="MKIKQTTVAKYAAVFAFAFAVNLAMAATTGAEFKGLADMVSGWS
                     EGYLGMSLGLTAFLIGLAVGLMKQTVMPCIVGLGVGLAATLGPGIIKGMFTLII"
     gene            26513..26794
                     /gene="traL"
                     /locus_tag="ACFMWZ_000027"
     CDS             26513..26794
                     /gene="traL"
                     /locus_tag="ACFMWZ_000027"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFC5934059.1"
                     /translation="MRDFHVPVSIDDPKKAFIWDLDVFILFMSGFGIGIVSRHFFISM
                     SISLFIAWRWGKLKSGKHQWFFLHVLYWYLPINEKNKRIPETNKREFLN"
     gene            26791..27447
                     /locus_tag="ACFMWZ_000028"
     CDS             26791..27447
                     /locus_tag="ACFMWZ_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFC5934060.1"
                     /translation="MKTENMLSELAIRTGIKVFFWILLGMSIAVNLFLSATLLFKGTI
                     VQTTLTPPEIRRSMTVSNIAFSKEYLEEMAPYTAYLLLNVTPKTVDYQNSQLLKIVSP
                     DYKDALERELSLNALWVKKNNVSTTFSATEATADISDNTVSIRGVFEVKRNNTLVEQK
                     DRELLISFKNNYGTLQLLSIKEVQKRVTKAEPAGQEKSEVETEDVEISKTATEFKGGN
                     "
     gene            27458..28192
                     /locus_tag="ACFMWZ_000029"
     CDS             27458..28192
                     /locus_tag="ACFMWZ_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFC5934061.1"
                     /translation="MCSLLKKIVFSALLVAGPTTSFAAQRVPATENTPHVVSISKRNL
                     SRIAIEGGRISSWKFMEGDLELQKDTTTGQLFVRSLTSSPTNLFVVSEEGKTYLLVLK
                     PTSKQGDNIVIDVAGANRHEAAVLASRTGPMPVTMNSTEYVRTIKKMMTSMMRGTTGD
                     MGINHSHEYQTIPLWKNTLFIQNGSYTAADMQGLSFTLTNLGTKQLEIREQEFYRQGV
                     LAVAVRKQILQPGEITDVFIISRLGG"
     gene            28194..29507
                     /locus_tag="ACFMWZ_000030"
     CDS             28194..29507
                     /locus_tag="ACFMWZ_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC5934062.1"
                     /translation="MRVKVNKFKLSAARKRQLAIAGMVAGSMAAIVGSAIYVIEGNKK
                     KSAEVTQVQNKQQILKTDFTSPQAGITDNSLWMNTESSKIEDANRKISELETMVQELK
                     EKENSSNPDTSKGLGPDGLGKPPAISGIGDNGQLPPAPPAGTLPNGVPPADRSIERKI
                     VSGSMSEAELQPGTTGAVNTGNPNENVRVSPQLKEAEWVKSKTPRMNIEVVEDGGKVV
                     VSQGKFRARDSYIPSGTFFRSVLLGGVDAPTGGEAQNASPHPVLMRVTDFAQLPNRFK
                     YNFRECFVTGQAYGDISSERAYIRLQNLSCVGTDGRAIDMPVKGYVAGEDGKTGVRGN
                     LVTKQGQLLANALMSGVISGMGKGVSEAFKVTNNTAFGSTTSIRGSDQYRAGIASGIG
                     GAADRLAEYYIKLADKVFPVVEVNAGRQVDVVLTQGIEIDTGETK"
     gene            29518..30237
                     /locus_tag="ACFMWZ_000031"
     CDS             29518..30237
                     /locus_tag="ACFMWZ_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC5934063.1"
                     /translation="MKKKILAVIAAITAFQTAFADVKTVESTLRKLYPNTTFSSVKAT
                     PMASIYEVTMGDNIAYVQENGRYFIFGALYDMQEQKDLTEMARSAVTQKSYSQLPFKN
                     AIKIVKGNGGKGKREFALFSDPDCPFCRRLEETLAGMTDYTAYVFMFPIKSLHPDAIS
                     KAEHIWCSKDREKAWNNYMLMDKEPAAGNCKNPVSENIALAEQLKVRGTPSMIHKDGR
                     RTSGAMPRAELEKWLNGAGAE"
     gene            30234..30815
                     /locus_tag="ACFMWZ_000032"
     CDS             30234..30815
                     /locus_tag="ACFMWZ_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HFC5934064.1"
                     /translation="MMRLKALSITAAILILSGCSTLTMSGIGGSEKFRCSNAWNSDDP
                     YCESISSNYKASVAGVLKDGMKRQVGQPYTDQSVRTLMLTQAYSSGTPVRSQSEIARI
                     WVAPYLDTDGDLNDQSFTYVVLNQGDWLIAHNQQQIIDEYRPIRLLGNGAKTSDSTVD
                     PSSTNSNNTNDRIPDVGVNLNLQETSGIPGTTR"
     gene            30825..33401
                     /gene="traC"
                     /locus_tag="ACFMWZ_000033"
     CDS             30825..33401
                     /gene="traC"
                     /locus_tag="ACFMWZ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFC5934065.1"
                     /translation="MGVLSKFKNWLAGSDDVDQPMEALHNNPVDPDFDHLSDYLNYGA
                     YDTKTELFTLMSVKGDKPLGMGFVFELNPFLGGSDDLISKWGNLYALLPEKTPIQVQI
                     FGSPDLRNYFNEYEQVQASRPIDDPMRSTFETLAKKRTEFWNKGTQQVLVENTAIRLR
                     NLRCTLSVNLNISPDNGVEVSKAVSLLARMRDALISMKIYHHTWDANDLLAWTTLLLN
                     PNRMFTGQQGEIDPVWDETKFLSEQMIETRTSIKVLDSGSGLRFGNRAAGDCVIAQCY
                     SANRYPEEFHLSNMGALIGDAIEANMNYTSPFLISMAMFKRDYDTSSNTVKLKAARAK
                     QTAESKMAAVMPEAAKIKRDYDICLEAFGKGGGGLVSLLHQVVIWERPENINLAESQA
                     VSIWQAQGFGLYRDQYLQLGSYLTALPMAIDKEVEKYLDSKKRWSTKTMTNAVCMSPI
                     IGEWHGLGRPVIGLFGKRGQAMGIDLFANPAGNYNFAIIGASGSGKSFFANEIVRNYM
                     GLGTQVWIIDVGRSYEKFCQMIGGQYIEFTYEAGLCFPPFQMVTDINEDIEMLKLIFS
                     LMASPSDSLTEQQEAKLQRILEEVWYEFGQEGTVDHVRERCLKCLITGSNEGGQPGER
                     DLSMVAVADQLYTYCSEGIYGSYFNGKLNVEFKNDLVVLELEELKTKPDLQQIIMQLI
                     MYRIMQGMYLSRNQYKIMMIDEAWALLGDTGTAARFIEEGYRRVRKYKGACGTLTQGA
                     NDYYKTKAAEAALENADFIFQLRTGAKSLKTIQDKEPFEVNDGIMQILRTLSKTDYYS
                     EIFVSTPIGYGVGRLYSDPFNVLASSSKAEDVEAVNHYRNMGLNTAEAIEAVLRERAA
                     TR"
     gene            33426..33974
                     /locus_tag="ACFMWZ_000034"
     CDS             33426..33974
                     /locus_tag="ACFMWZ_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934066.1"
                     /translation="MTEAKEKKENTANTAPEKNPEDNGVEVSLGSLMIGAISIAALAL
                     GVWNFMKTNERPVHIVDLAGISMTYQEQAKNQGLQNGISDQQRAAILQNYQAKMNTLQ
                     QVIEEKVIQCGCNIFVKSALVGRNTEVVDVTADIMKELDKRVPATTVVAAPVHQQVEK
                     TTPKPMAEEGKGTPVTEEDMVR"
     gene            33974..34519
                     /locus_tag="ACFMWZ_000035"
     CDS             33974..34519
                     /locus_tag="ACFMWZ_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HFC5934067.1"
                     /translation="MFEAMIKISQKESNKAVMKRLYREFCQKYATPFLAVVVCLSAFN
                     QHYALAFNLSHSLPHHLYFIKKDANKLGDLKQGDYVAFAWQGGFYPIGTQVIKEVAGL
                     PGNHVTKTNRTFFIDGKEVGTAKEYSQEGMKLAINPFEGEIPQRFMWVKTGHKDSLDS
                     RYELSGLIHSGQIIGKAVPIF"
     gene            34528..35184
                     /locus_tag="ACFMWZ_000036"
     CDS             34528..35184
                     /locus_tag="ACFMWZ_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFC5934068.1"
                     /translation="MSHTKKIASVASLVILATGISSWVAASTPSVEKIGDKIGTTYPI
                     AEKHAIREIEARLKAKQDSGQLAAMQKEVQDRINRSALNLQPLEGLAKATKGSVRYFD
                     PSYTLTETIYDQDGQIIAPAGTVVKPLEVAPILFKMFFFDGREPDQIELAKKLAAEYG
                     ESFMPILTAGNWYELSLEMNQAVYYDQEGRMSKSFQLNEVPSLVSQEGDRLRVEVMKP
                     "
     gene            35181..36299
                     /locus_tag="ACFMWZ_000037"
     CDS             35181..36299
                     /locus_tag="ACFMWZ_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFC5934069.1"
                     /translation="MKIFVTPLKLLIPLLLLPLFSISHAAEAVPTTPISNKLTCHGKM
                     LNPINDICWSCMFPIKIGGKVNLNMDNQEDNDSSPSNWFCSCTHPIRSGVTVSFWEPT
                     HLVEVVRTPFCMVSLGGISLKNQKADKAETTGKADVSNKIQNLFGGSHKRGRNEQHDQ
                     HNAFYQVHWYKNPVVFWLEALLDNDCIEKGTLDIGWMTELDPTWKYPSLAVYQAPDSA
                     LFANVVAQAACAADCVKAATGFPFNKLYWCAGCHGSIYPLSGWMSGHFSEVQAAKLMV
                     ARIQTKMHRDLVAWRTSGKDALCGYKLNPVMDKQNYKMAMTYPVAARKFRGRCCEPIG
                     RSTLVRDSGKAFPYKGNQFAFQLFRKRDCCGAYKFKTN"
     gene            36314..37066
                     /gene="trbC"
                     /locus_tag="ACFMWZ_000038"
     CDS             36314..37066
                     /gene="trbC"
                     /locus_tag="ACFMWZ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFC5934070.1"
                     /translation="MDKSLRNVIVAGGVFLILLGGLAGVGSAYADADVEAARNRLRTT
                     MGKVTQVKNPNVGDNRQQPEIPDLEGIKHRVPDVRDYGNAKIKADPMSIAQKYRPASQ
                     EARQAAEKADLMVFVSFSMPEASLKRIARETAKAGGVMVIRGFKDDSLKATVKAAEEL
                     AALQGDMLIHPELFDHYGITEVPVTVIARNSEDGLDGCAVNSELGMCTEHVAVKGDVS
                     LHAALEHLIRESKDKKLSDIANAKLAVLEGRK"
     gene            37063..38265
                     /locus_tag="ACFMWZ_000039"
     CDS             37063..38265
                     /locus_tag="ACFMWZ_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934071.1"
                     /translation="MKRHAIYLTVFGLMQTAFAADYGSYVGELGKLGTGTATNMMQPA
                     QYTPRYTDNVPMAEQYYGGGVSLPTQFGDAKFNDCKNLPANPDLYLRQECEGVNLIAG
                     SRTKRPDVTISSNEKLVTGTQKIAGDPAETLEKYKWKYPVNEDGSVGTVPSSACPTET
                     ITVPAVKTIKRCNEYHGAEMFLCEAALKVNVDPSWNYSCLESKYRNQQYQCGKKLKVV
                     CEKSGPNCTRSGIEAGSLQGDMTVNMTPSGSAHRLMFGTIGDNYWRDGQYDREMTLNI
                     KNPEKLTLFTLSRVEYDDWLIVKVNDKIVYSSYNNQMFAVDYTAEDEEGFPIGRRYAP
                     VRNEEGTRLGYAEIGESWRRNIIWTRTVVGGGGENAIFFNVQQYCDPVCHDRWENSCL
                     EYEQRVKQ"
     gene            38289..39845
                     /gene="traN"
                     /locus_tag="ACFMWZ_000040"
     CDS             38289..39845
                     /gene="traN"
                     /locus_tag="ACFMWZ_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFC5934072.1"
                     /translation="MICHFMLITSASAAALRESAACTRTSSVCVDGPSTKNINGVDVT
                     KDCWEYKEEYQCLEKDSADYCAPLKDPSAKCEVQGQTCLEQSNEGECLRYTHKYSCDV
                     DLRTLHQGRLPPKVEEMEHTHLISSQWDESSCQVQGKKCKAVATECLEPGSTKIINGV
                     PVTRDCWKERRTMQCTDGSDSETCSTYTSSDQCRLIEDKCTHQLPDGTCQAREKQFEC
                     TEKGETTKEVSSCQDRDFAKTMTMMEFARETQRFYDPEKQRFFNGEAGQCSIKLNGAL
                     DSVFGGDCCRTKAEPGKFVDFAVQTGTTMATTYFMASVASHYTFTTMFVSSAAQAMGT
                     ALSAAGGITGTSQISALGFSAAGQQGMGVIVGFNPVVFAAAIAVIAIQQWLKCPKSEI
                     LVAMKRKADLCHYVGSYCGNKKLGVCVTMIESQCCFISKLAKIVNVGGKEQLKRDWGT
                     PENPKCEGFTAQELEQLDFSKLDLSGFYEEIYANMDNVAKQEQKVSQKIRETSVNGKN
                     LEVKNYYEYQ"
     gene            39895..40257
                     /locus_tag="ACFMWZ_000041"
     CDS             39895..40257
                     /locus_tag="ACFMWZ_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934073.1"
                     /translation="MALAASLPYTGGIALEKTEALARTSIPLPDGKPDALLALAMRHG
                     SAKGYFSGRAAAAVKQKFRQEIPIFVEAVRMEEVKNKPRCNRVRLTFHTSPALLQTFP
                     SEIMDIEACPNRKNGGKK"
     gene            40269..41045
                     /locus_tag="ACFMWZ_000042"
     CDS             40269..41045
                     /locus_tag="ACFMWZ_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFC5934074.1"
                     /translation="MTVMLIFSLHASNLRAADDGMGWYDQDIWKNENRAFLWYPDAKK
                     PKQARQNQSQNTPVRNELAEFEMLQRNLDESRKIAVMNPTPQNLKRYIELQEVVMNKS
                     AAFADQWQRVIWQNPNLDYSQRGRPNNQLAQQQYDHNRQQQKAEAIRKLAGENGIMFV
                     FKSDCPYCHTMAPILKNFADQYGIKVMPVSLDGGGINHFPRALPNNGIAQRLNITTVP
                     AMYVMDTKTKQFTPIGFGVMAQTTLEDRFLAYSRPVGTLY"
     gene            41218..42729
                     /locus_tag="ACFMWZ_000043"
     CDS             41218..42729
                     /locus_tag="ACFMWZ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFC5934075.1"
                     /translation="MKFHIFRFPKPAKVLAVCVSSAKYLCLTASLTLPINSSAGIEKN
                     MADMFNSMGAEANYTEAGAFHGQSGNLYTGGSMSVRFPVSDISLGNIQLPSISAGCGG
                     IDFFAGSFSFVNKEQFIQFTRNLGNNAAGVAFDLALKALDPMIQDAIGGIRDLVNHVN
                     QNSLNSCKLSQDIVGGAMGKLGSLASSNCRAASVDSGSANDPAEGTWYCQAARNLVHE
                     ADKVRNTGKPQDTISYTGGNLTYEAMKNYTGQRAKLGIDMDFYYSMLGTVVFTPLTQH
                     EGDKDKYTVGVQSFEPKILSIQDLLNGKNNAGGEVREKIVLDMWTCKDGSKKLKENCT
                     EKNNVEIPSLRYMVHKRIETLPTAIRNNQGWNLSTRKQIAAIVNNSKLPILKMAVSDA
                     FLGTGYLKKTAVLDAIAVDIMANILDQSERQVRSTLGMYNKTDDASGEAVRKLFANLG
                     ELRSRIYQERIDSMKKIEIEESMMRAMEQFETQWRSAFVDTNNSMTFDSINRF"
     gene            42738..45629
                     /locus_tag="ACFMWZ_000044"
     CDS             42738..45629
                     /locus_tag="ACFMWZ_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFC5934076.1"
                     /translation="MAVEYFTFPGNGEVVQAVFNGVAMISGSTMMGGAMQAAALFGFL
                     VALSVAVFKLDLRESFTYLFVVGFFWMGMIVPKTTVLITESGGYGYTGRQYTVGNVPV
                     GLAYMGYFVSSFGQSITRKAEQVNHLPDDLNYSRTGMLFGTRLLENIREARIPDAQLT
                     QDWALFMNQCSFFDMNLYHFYNVQDLAQSADILATLTHTNQALFTNVSQIASRRGNML
                     QYNGKSKTMTCNEAAKELKDRTQFYSKNYLPGYIADRVFAGLGTNAAGINRVNALVTL
                     GNSSFQYLLDNARFDTLKNIEQAAMVEVIRQAGIINGQRNRNSAAVQQAFAQVQARNQ
                     YIAAQKTGSSMASWNLPLIRSAAEAIMIGLFPFVMILALLGGIMAFRMLGFYMMGMMW
                     IQLWAPVASIINLIMTMNTKRLLSLETLNGAITPGSSDTLLMAAADAQAAAGSAMWLI
                     PVIAGALTMGGRSLMNGMMGMTSSAKSTGEAAGSQVGSGNYSAGNMNYNNSNANKHSL
                     NPVYTDPQMMTAQSAAGTSWRNMATGENRYQGRGDSFGNVSSTSTVSMSDVYTQAADR
                     STTAAKQYAASSVEQTTLGNTQTLAFGLQTVSQNGANYAVTNGMSSSTAKALDQIFQS
                     SKDIAQRNGLGNDVQTASSIALDLGAKLAGGATTPYASATVSGGVKAEGRGGSTNKLS
                     HDISETAKALKSQGVSITRSMLENIGQSNAFTQALTDSSSLASQAQASYNKAHQASIS
                     EQQSYNEAQNYREAASRTAQSGATVSIDNAKAIREYADQNGLAYSQVYNNPSIVAAAA
                     MNAAVTQASNLRQQAEGTRFDSTGPISPDDRPTNNVAAAYSSYKSGIDGQKSSTFATI
                     QAQADRNGVNRNEARANIDGWQQKLEQGSKDAKNFNQKLFDKGMKKQEENTNKLHAQG
                     EDTFKHVNTFAMGATQTFSSDKAYVQAQNKYGKTEGRPGSGSRNYRPKK"
     gene            45692..46135
                     /locus_tag="ACFMWZ_000045"
     CDS             45692..46135
                     /locus_tag="ACFMWZ_000045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC5934077.1"
                     /translation="MGPLLPGAHTIISPFGKRVHPILKKVINHKGEDYRGSINTPLLA
                     PFSGRVVESRYQANTVNGKTVGWGHTVVIEAPNGVRYRASHMNGASPLRFGETVSKGQ
                     QIGNLGASGGVTGPHLDAMLYSPSGKILPVSAYRGKTLNEIGKIK"
     gene            46151..46315
                     /locus_tag="ACFMWZ_000046"
     CDS             46151..46315
                     /locus_tag="ACFMWZ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934078.1"
                     /translation="MSIAHIVIEIEESSTLDDCNVRLSFSGGTELQSDEAKTVFYKTL
                     EAIEGVLGDD"
     gene            complement(46353..46934)
                     /locus_tag="ACFMWZ_000047"
     CDS             complement(46353..46934)
                     /locus_tag="ACFMWZ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFC5934079.1"
                     /translation="MIGFVKWYSYEKDFGFIFGEDGHEYFFRKSTGSDIPGQIVNFEP
                     FEHSRGLRARKVSDIHNPDPQQFQKLKTLCKDFQKVEKQPMSKKDNLLSAAILSLCLT
                     MFITLGFFYILAGFFDTDRETNIVLLFVFPAVTFSVLTFFLNKIFRNMKLGSDADVVT
                     NNDWLSEDSLNPFIYDPAYSNLSCNIFHRSNDD"
     gene            complement(46947..47561)
                     /locus_tag="ACFMWZ_000048"
     CDS             complement(46947..47561)
                     /locus_tag="ACFMWZ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934080.1"
                     /translation="MKKIMFGLLAAMAISNAQAEGNFLNLASKPAKIDGVLEAFLDKP
                     YPKFVQDWMKDSKLAGLGRIIQGKIFESTGTIERKFYARSESEHEKTVEDAVGTFKAY
                     CEHHQGTVETLTSDIFCINGRDVQWMGWWYRENYTNFAFSEGYTGEQTYRSKVTDVQP
                     GKKISTFYGPALVLEKGENGLIKIQPNRGNERWITSDSIQVTYK"
     gene            complement(47614..48564)
                     /locus_tag="ACFMWZ_000049"
     CDS             complement(47614..48564)
                     /locus_tag="ACFMWZ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934081.1"
                     /translation="MKIIAYTSPDGDEWVTALEAKLKHREGMIYYSRHRPGTRKKMVL
                     TRRKATNFFRYYSEAESGGASAPESLTHLLCKQVLNELSNLPGGLTTVLNYTEHTEKQ
                     PPVTIRLNRALSEYRIEIDGKTFYIDVLLEFDQPGNSSLLRHEIRWQRKLAVEIWHTS
                     RLASNAPKCLALSKIGIPVVQIRADKSSFLYIDEDELLNYDNEEIKIRINRHVEKLRN
                     TFRKQILCTLLRNPLSADFQTALMLHNQIKADEQQVEQIQEKFEALRNKHVLLEAEYS
                     ALVAQYAALLEHQNFQAHSEKREIPKKHGILQRMASWFKS"
     gene            48856..49554
                     /locus_tag="ACFMWZ_000050"
     CDS             48856..49554
                     /locus_tag="ACFMWZ_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC5934082.1"
                     /translation="MSSLKIARIYLRVSTLQQNLERQRKIIDEAKENGFYIAGIYEEK
                     ASGVNASRPELNRLIDDLQEGDWVIAERIDRITRLPPREAELLIQRITEKGAKLHVPD
                     VFSFGNLDELMGEGDSLPKYLTEPLMEALQKMFLRMALSIAHEDWKTRRERANQGIAR
                     AKREGRMKGRPANEDLHKSIIQLTLNGVSITGIASALKCSPSTVSKVRRAYNRKHKSG
                     DKDQFDIFKPSLKK"
     gene            49992..50573
                     /locus_tag="ACFMWZ_000051"
     CDS             49992..50573
                     /locus_tag="ACFMWZ_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFC5934083.1"
                     /translation="MDDHSKKRGGRREGAGRKSKSGEATAVKRIPISLIPTVDAMIAS
                     TAMPQGSLIPINRTTLKVPYTLEKIPAGFPSPAEPYIADYLDFNEYLIANPSATFAAR
                     SGGYSMLDAGISKDDIMIIDRSKTPKHGDIVMADVGNEFTIKRLYKIPGRKPELHSEN
                     SSGEYPDFIPNDSDTWTVVGVVTFVIKDVRQGS"
     gene            50575..51825
                     /locus_tag="ACFMWZ_000052"
     CDS             50575..51825
                     /locus_tag="ACFMWZ_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFC5934084.1"
                     /translation="MFALIDGNSFYCSCERVFRPELKNIPVVVLSNNDGCVVARSAEA
                     KALGIKMGEPYFKVRHLVGQGRLKAYSSNYELYADLSRRMMETIAGCVPAVEVYSIDE
                     CFADMTGIENLAVHGQQIRSRVLQWVGIPTCVGIAPAKTLAKFCNHLAKRYPDYFGGV
                     VVWDNWNETTRLRALRSQGVDEILGIGRRTADKLSEQGIRTVLDFVEADTATIRRRYG
                     VTVERTQREMQGIVCDGLHLNAQPRQNLVRSRSFGKRIGTLEALQAAVTHHVSSGAEA
                     LRKDGLTASAVSVFLHTDFFREDSPQYHNSLAQNLIVSTADTVHLNRIAQRLLADIYR
                     SGYLYKKCGIELYDLKPAIQIQADLWKPTKANPAMEAWDKINRQHGRGSMKLVSELLA
                     DDWKMNRDRLSQCYTTRFTDLLVV"
     gene            complement(52132..52581)
                     /locus_tag="ACFMWZ_000053"
     CDS             complement(52132..52581)
                     /locus_tag="ACFMWZ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HFC5934085.1"
                     /translation="MPDSPMQINGYTIYEHECFTQQLDRLATTIESQKRSEKSSRSEE
                     TKLLERILDEIENIALNPDSPEYRLGKTLGSEYKFWRRAKFMGRYRLFFRYDSNSKII
                     ILAWVNDSGTKRTYGGKNDAYTVFKKMLGNGNPPSDWTALLEVVSKE"
     gene            complement(52583..52894)
                     /locus_tag="ACFMWZ_000054"
     CDS             complement(52583..52894)
                     /locus_tag="ACFMWZ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFC5934086.1"
                     /translation="MKIIFDAVSTMTSKNQTTIPESVRKALGLEKQDKIRFSVLGDGQ
                     VLLEKNNTEEEEFEHDPVVGKFLHFLENSMTKNPNSIKPTSQSRFNRFRQLTWETDMK
                     D"
     gene            complement(53281..53763)
                     /locus_tag="ACFMWZ_000055"
     CDS             complement(53281..53763)
                     /locus_tag="ACFMWZ_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934087.1"
                     /translation="MIDNQIDKEVTQASCEARFILKQENGKRILYLNLPDGSDELNTI
                     WQTDEYDFTVPDLEVSIDVESLYTAVRLLNENQGILHSISAKCSADSFGFEGKLRYER
                     LDVKPFPIKSFSYYLEFYNDWTGTLYELDLSAFLDEFFGECDPESKLDACLKQHLCLK
                     "
     gene            complement(53850..54737)
                     /locus_tag="ACFMWZ_000056"
     CDS             complement(53850..54737)
                     /locus_tag="ACFMWZ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934088.1"
                     /translation="MCAGFRWRLFYQPGRKPINLRVSLSAGMGIPALETTMPNWCSNS
                     LHFQGNPERIAEVAEQILVKTDKGYQFSFQHIVPMPEELNLTKGSDSHMQQTWLQIAQ
                     QPEKKLTNLEQEMLEQRDEPFWPVQYRLPDELFSELKQYFSEKFVWDNLTVGEVLAHF
                     AESPEVTTRFRYDEPFVKRIAENIRQYGHPDWYDWRLANWGCKWDLDPDCTFVTVEES
                     DIWISCDTAWGPPDRLYKEIAKRFPDVEFEAKYLEEGMWFAGSYEGHEGVLFDYPCEE
                     DEVRDFAIEHFGCEYDDDD"
     gene            complement(54942..55181)
                     /locus_tag="ACFMWZ_000057"
     CDS             complement(54942..55181)
                     /locus_tag="ACFMWZ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934089.1"
                     /translation="MPDNMKSKTKVPTAVLASWAAILDDIAKAAIIGIGVVVWSNGYS
                     EEMKNLAMAGLVLLSFEMLYVSFLIRIHISKLEDK"
     gene            complement(55332..55847)
                     /locus_tag="ACFMWZ_000058"
     CDS             complement(55332..55847)
                     /locus_tag="ACFMWZ_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934090.1"
                     /translation="MSTKVDNIFLIEGSKESVAEAAKLILFRSHAYPEHRAFDFDRIV
                     PLPENITSDLTKQRIEAWGSSFSPCPTETFVTQREGFIEICFLTGLAPPFGIYRKLAE
                     IFANLDVCFTAKYINQYGSFGGRYEYRNSVLYHCLCEMADIRKFGIAEFDIWYEGLSD
                     RLESDGYLVED"
     gene            complement(55932..56519)
                     /locus_tag="ACFMWZ_000059"
     CDS             complement(55932..56519)
                     /locus_tag="ACFMWZ_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934091.1"
                     /translation="MQNFIVWMNPDREESAAAAAKLNGAVLTAHTDGVMVAIRRELKR
                     FGLGADLAVFAQTCEEFAKQANADMVCISSSNGDAVAAVTYLEDGYVGEGVSAVADMR
                     YLMSAYRQYFNRPDKMVIFQGALQYLSDEDIAWIFRRHSNAIIEWQSNEGLGLYEFGF
                     SLSDVLTDGCRNVLREVVTGWLHREILPVLCAEPT"
     gene            complement(56747..57496)
                     /locus_tag="ACFMWZ_000060"
     CDS             complement(56747..57496)
                     /locus_tag="ACFMWZ_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934092.1"
                     /translation="MSVSKYFMLRPSAMDWDFIEKHYPNYYRSNAILKSDDMSYCLGD
                     MGDVPRDTVITEESYKAFEEYAEDLAYRVENGFADFIGSSLTFGQAADYFDDWLYAVA
                     VLEGVGCIQVSEAVRFWAEKVFNTPAEKRRQWYAGRLSSDPKPSDAERLLIEVANYIA
                     LKYGLVFGENGESVLDVSENILQAHLYHEADPGFSFFLKSDQFGLSVFGSSRYFPLQS
                     IVISNEMPIAAAQTLIDILISGSAPNTVVSG"
     gene            complement(57567..58154)
                     /locus_tag="ACFMWZ_000061"
     CDS             complement(57567..58154)
                     /locus_tag="ACFMWZ_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934093.1"
                     /translation="MQTYIITMDMENELSEAAASALNGRFFTCPQNYQAIWDQVSDYL
                     SDKGFPHGIAQDVFEAVEYFTQNFHSENWPSKDLNLFAAAVSVEHAVEDQGCADLLHL
                     LRNKDDVEASFEDYVQDLTGKDLQWVYGRNRDAVNHWLAESRTDLFDLLESQENLAEV
                     VTVLWAKALLGKYFDKYGRNIPSSSQLEADEAVSA"
     gene            complement(58263..59132)
                     /locus_tag="ACFMWZ_000062"
     CDS             complement(58263..59132)
                     /locus_tag="ACFMWZ_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934094.1"
                     /translation="MTRILRDVRHFLLLTPWGPYPWGTVSHFSRSNTVKKNIETYIVW
                     FKKEFEVAGKAAEALNGQVICYPSNDESLTDKIGCKLAEMGYSANLISAIVPVAEYVR
                     MENEMGSVPNMTENRHTAAVSFSVMNDHDAWTALLRQPGFADMLTDDQAQEVFQSIMR
                     GRDVGYSMLMEALFDSGMPDNDSFVLVPVPQIGGYDDIAYLLKDVEYGPSDHIEFLEQ
                     VVSEKTAAWLFERNEETVLQWMEENGLNWGNFTDLNTSRPESKGKVIHKAIKSWAIDT
                     VRRYLNSHPLQNS"
     gene            complement(59261..59602)
                     /locus_tag="ACFMWZ_000063"
     CDS             complement(59261..59602)
                     /locus_tag="ACFMWZ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934095.1"
                     /translation="MEQLDLAAMAACDTPYLFRLGRVSMTATVDNWAKEHLPAQLYDN
                     DLYAQILLEAHRRGIWGDLPPEDAKLNQLALKPGEEGRIMSSYKIGDQKIWVITEWDR
                     SLTTLLFPEDY"
     gene            complement(59684..60805)
                     /locus_tag="ACFMWZ_000064"
     CDS             complement(59684..60805)
                     /locus_tag="ACFMWZ_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HFC5934096.1"
                     /translation="METQNHLMHPRVAHNHRDNGYFPTDQATLARIAACLDIPASAGG
                     LLRIFDPCCGTGEALASLASTLQSEKISCHTYGIELEKNRAQEAMSRLTSVIKSDVEN
                     CTMQGSAVGLLFLNPPYGFAAKDNLSNQRTKRLEELFFARTFGTLQPGGVLVMIVPEA
                     ALTEHLTGEIATHCTDVRMIRAAVDTYKQLVIFGIRPKNKASISKKQADAQQRLLLAF
                     ENAPETLPTGMPAYCVPIAPNKVFRPKSFKLEQDILERELGQQQSRTLWPSFSQHFGT
                     NAVIAEKRRPLCALGQWHAALALAAGQVNGVVTSTNGRKLLVKGSTHKTKVETKSEEY
                     DNKDRLVVTITSTDRFSPSIRTIDLTEGSPDFGRVLTIK"
     gene            complement(61661..61918)
                     /locus_tag="ACFMWZ_000065"
     CDS             complement(61661..61918)
                     /locus_tag="ACFMWZ_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934097.1"
                     /translation="MKFFIFIKPDLPFNKESIESWIKLLEDIGKTALLAMLPLFWLNS
                     YSLTERASGVVVLALVAYFVQVYADKLRKYRDSLNRKDTSC"
     gene            complement(62166..64649)
                     /locus_tag="ACFMWZ_000066"
     CDS             complement(62166..64649)
                     /locus_tag="ACFMWZ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFC5934098.1"
                     /translation="MYNPVSKIDELFPDFAELDQTVTEMQERQNQITTDFNFIIRQVA
                     ETAQKSTEAAVLPEESPETELLDSDIPLTQFLAEFGDSLFNAVTEQNQPVYQGQTYPL
                     LEEVLDNLERPLFAAQREVVRAVLQQLVVEDKPAAIINAEMGTGKTMMSIAAAAAAHQ
                     AGLHRTLVLSPPHLVYKWRREILKTVPNARVWILNGADTLAKLLQIRALDRTPEVPEF
                     FVMGRVRMRMDYHWRPSYAVRRRWIPSEGASEGFAVSEYYCPKCGCEILDEENKPYNT
                     EGGVKNALAKNRRFCKGNHHGKPCKEPLWTLCRKEQSEDGVVSTVYDRVLKTMTTLPG
                     IGPKTAAKIIGMYGEQYLAEILENNIQAFSNLMDENGEFLFDDRQAARIDRDLGKAEF
                     SLGRGGYQPTEFIKRYLPKNFFGLLVVDEGHEYKNYGTAQGQAMGVLARCCNKILCLT
                     GTLMGGYAEDLFFLLWRLWPQMMIEDGFSYNKGNTLGSASMAFMRKHGVLKDIVRHLG
                     TEYSNGAFSSSKAERNSVRTAKAPGFSPLGIMRYVLPITVFLKLRDLGEGVLPGYKEV
                     FRPVEMTEDQQAVYKNMESVLREHLKVALRNRDNTLTGVVMSALLSWPDCCYKPQDVY
                     WRRQNKILFHTEPQFDEEEISPKEADMLEVVKDSLAKGRKCLVYTVYTDTHDTTGRLK
                     EIFKRHGIKAAVMKATVKADQREDWVEDQLASGVQVVICNPELVKTGLDLLAFPTIYF
                     LQTGYNVYTLMQAARRSWRIGQKEDVEVYFAGYSDTAQQICLELMGQKVAVTQSTSGD
                     MPDSGLDILNQAEDSVEVQLAKRLVDRQD"
     gene            complement(64745..65236)
                     /locus_tag="ACFMWZ_000067"
     CDS             complement(64745..65236)
                     /locus_tag="ACFMWZ_000067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC5934099.1"
                     /translation="MPLPDFRIRLLDAADDRNTFECPSEPLNRYLQKQVSQDVKRKVA
                     TCFVAVTSDGQILGYYTLAATGVALSNIPAGFRKKLPRYPTVPAVLMGRLAVDGKYGG
                     FGLGSLLLADALKRISKAEIAAYALIVEAKDEAAIGFYRHFGFTSFEDATKHLFFPLA
                     NLR"
     gene            complement(65238..65522)
                     /locus_tag="ACFMWZ_000068"
     CDS             complement(65238..65522)
                     /locus_tag="ACFMWZ_000068"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFC5934100.1"
                     /translation="MFMTATYADCRFEARITSDTRALLKHAAELQGRSMSEFVISAAR
                     EAAQKAVADAEILMLSERDQLQFAQALLNPSKPNGALSKALKKHDELFGR"
     gene            complement(65862..66329)
                     /locus_tag="ACFMWZ_000069"
     CDS             complement(65862..66329)
                     /locus_tag="ACFMWZ_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934101.1"
                     /translation="MQYLNEKTLEIRILETDGLYGCDVFDTEGKPLAGVIPEYKSQVE
                     AVAAALDIPGVGLFTDTDQRSKQKLVAVTDALALKYGLNFKNHGGVYRKYDNSHACLN
                     KNGRKGFVLFFTANDKFLTLQGSTCINPEIHYQFDIYVDNDFIVEKVSMLIGS"
     gene            complement(66459..66770)
                     /locus_tag="ACFMWZ_000070"
     CDS             complement(66459..66770)
                     /locus_tag="ACFMWZ_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934102.1"
                     /translation="MTIMKPEQEDLQAAFEFVGMMTAVARHELNPLEKDEFDDLRFLE
                     DEDKAKVLDALCEKFNNCDLDWLMIALAHLLSPDRGVIDQDSDILTINPNLLGATDKS
                     N"
     gene            complement(66784..67158)
                     /locus_tag="ACFMWZ_000071"
     CDS             complement(66784..67158)
                     /locus_tag="ACFMWZ_000071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934103.1"
                     /translation="MYGFFFYQPYGDTSPIGDIPLFLEIVMKFDTNNACTDAIYRPEN
                     VFQQQDVREILLGQYSYAGSQILRNIVRSLYKGVPVELYQVSQLDQKHYAIFEAILAH
                     YRKYGESEQLRQIVCEMNREAG"
     gene            complement(67373..68071)
                     /locus_tag="ACFMWZ_000072"
     CDS             complement(67373..68071)
                     /locus_tag="ACFMWZ_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934104.1"
                     /translation="MGTSNFLYNHKLDVISGTSFSTFDLEAYNKEMDEVDCLKADDYE
                     EIGEILSHNCEDAVDGIKYRAEESGNRKYRQDGFLVDSTTGEADDNGEYNTRCFGGTR
                     IATISVETTFWGVSIFMEMDVIVRCGYYDGNNIDQSFRIFTDYSNYCILDGYDTSSFE
                     EAVDYVIGELEDADIATKRQLYPHQKGIGRRLCALEQVLWQRYEELIAPWCEEYQVSA
                     RFSNGETWYSKKAA"
     gene            complement(68325..68906)
                     /locus_tag="ACFMWZ_000073"
     CDS             complement(68325..68906)
                     /locus_tag="ACFMWZ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934105.1"
                     /translation="MLYLTSRNDLMADAFFIWNRQLMFASLHGRDADMLSFQAQLQVS
                     NERLGFRQPEEVLSCPRLTTAEYCLNLSKFMTKYQTLNYGSVTHMFLYSDILIQPNFD
                     SKTGWVMLDDVTADLDKAAWQLVQQLSDIPLLEHWQNAVLSLLEADKSIMRFTPRIDE
                     EYAVVGVQAVRVDIPEDFDVRLTAMLQSGRLHT"
     gene            complement(68916..69458)
                     /locus_tag="ACFMWZ_000074"
     CDS             complement(68916..69458)
                     /locus_tag="ACFMWZ_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HFC5934106.1"
                     /translation="MSMTIQGTLTVQVIQGRRGPFKTAKLATSIGTFDVKNPILKQFE
                     PGNYEGNFILNQLKVKTFEWGGGATSYIDAALDWQHLADFAQECGLSAEVEEQSSQAS
                     TVDPVSESGAFDPVSENGIEFPEFAETLNQVEQMIENKCSHISFGEYILADREDLKCA
                     VELVREAGYSYNPREKRWYV"
     gene            complement(70643..71089)
                     /locus_tag="ACFMWZ_000075"
     CDS             complement(70643..71089)
                     /locus_tag="ACFMWZ_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFC5934107.1"
                     /translation="MTQTNQNYFNLTANGIGFLNRPREVQGRKSSNYYACTIQASRGD
                     EGEKTRFDLRIVGGEAKELFARLLGEFPQLLSKNYRERPTVVVGFRAGDIQPLKFEAT
                     NHKTGEVSELLTIDGRLLKFKFIKVDGDMWYQSSSQDETQNNSDAS"
     gene            complement(71353..71955)
                     /locus_tag="ACFMWZ_000076"
     CDS             complement(71353..71955)
                     /locus_tag="ACFMWZ_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC5934108.1"
                     /translation="MIGVIGLEFQMDRRNFLKKFVGVGALYTIGAAGLLLPKDVVAAT
                     SMADFWSRDRTINCKRADTGEKHEIRFFQQQNGYDLDAYRNACWLMRDAKDGNAMVQI
                     DVGLLNLMYAMQEWARQSGRPNPVITINSAYRTPRRNATIEGAARNSLHMHGKAVDFT
                     MRGVSISELEQMAKYYNVGGIGIYNSFVHLDTGRVRHWRG"
     gene            complement(72587..72760)
                     /locus_tag="ACFMWZ_000077"
     CDS             complement(72587..72760)
                     /locus_tag="ACFMWZ_000077"
                     /inference="COORDINATES: protein motif:HMM:NF040300.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFC5934109.1"
                     /translation="MYYHIIPKYFADTSDREIKLASLEIPELGLKLSDKELKTIKPYP
                     NKKIRSILRLIVI"
     gene            complement(72815..74854)
                     /locus_tag="ACFMWZ_000078"
     CDS             complement(72815..74854)
                     /locus_tag="ACFMWZ_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC5934110.1"
                     /translation="MRLFLCEKPSQAKDIGKVLGVLGGRHDGYFQNGDTIVTWAFGHI
                     LTQAAPDAYGEQYADFGNISALPILPEVWQMQVPPKVSKQFKVIKSLLAKADEVVIAT
                     DADREGEVIAREILDYCGYRKKISRFWTSGLDTQSVKKALSSIMPGKDKENLYQAGLA
                     RSRADWLVGMNLSRAYTAAYSAGYGREHILSIGRIQTPTLKLVVQRDKEIDTFVPYNY
                     YTLSVDFETADKSVFTTLWQIPENWRNTDGYCTNLEAVKALVKQLEKSTGKLITSNKE
                     RKSTPPPLPYSLSALQKEAGKKLGLSASKVLKIAQTLYENYKITSYPRTPCRYLPKSQ
                     MSDVKDVFAAIQTIDKSMLSVIEKADPERQSRVWNDNQVNKHSHHAIIPTKVSNFDLS
                     VLDKNELTVYRMIRDRYIAQFYPDFEYDSTVVEVEACSHLFKASSQLPVISGWKVLLG
                     KDVFEGDQIDEGPALPHLKVNDEVDTLSSNPETKKTTPPPRFTEASLLDEMQTLKDFL
                     KNVEDEQIRKILKSTEGLGTEATRAAIIDRLFEMGYMEKKRGKIYATEKGRNLIARIP
                     TMIADPITTAKWELALAGIEAGKLTLAEFMAYQQKVITELVGQAKKDAVGKARPPKQT
                     DSAGTKKQAVARNEDDVCPTCKEGRLRQRGFKENPDKRYWGCSRFPECKHFEWVK"
     gene            complement(74856..75041)
                     /locus_tag="ACFMWZ_000079"
     CDS             complement(74856..75041)
                     /locus_tag="ACFMWZ_000079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934111.1"
                     /translation="MATKNTSFFREMNCWFAALWADFKDMFIVTLIYGGLIALAIWAF
                     LTIASWLVLQLKQSLGF"
     gene            complement(75046..75477)
                     /locus_tag="ACFMWZ_000080"
     CDS             complement(75046..75477)
                     /locus_tag="ACFMWZ_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC5934112.1"
                     /translation="MSVQLSVRGNLGAEFNLRPVASPKNDEASIVLNFSVASPNFKRN
                     AEGKSEVVSTEWISCEYWNRDAAHLHKILQVGMPVFVTGEERYETYTNRDGIEVRVRK
                     LRVQDVFIVPTERVEAIKLRPKREQSQDNNHVEDADDDIPM"
     gene            complement(75786..>75989)
                     /locus_tag="ACFMWZ_000081"
     CDS             complement(75786..>75989)
                     /locus_tag="ACFMWZ_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934113.1"
                     /translation="ALDNTNASLDERLLHMKVYFDCRQYFCRGRQIAGDLAELFSMKE
                     IFDEILKIENLDRKNLPSNIMVK"
BASE COUNT        20998 a        17455 c        17777 g        19759 t
ORIGIN      
        1 tggctgcatt ggacgtttta atgtttttct gttttgtctt gcgccgctcg aaacgctttt
       61 tgcaatgggg cgatttgtgc cagccgcgcc cggtcgattt gttcggcgat aaggtgcgct
      121 tttccctgcg cgcggcattc ggcggcgatt ttgcccgaat ccgcctgatt ggcggcttcg
      181 agtaaggcga gccagtgcgc gcgttgcggg tagggcgtgt gttcgcggtt cagacggcct
      241 tgcgtgtcgg caatgcagac gttcaatgcc gtctgaaaac gttctgggcg gcggaaggca
      301 tcggtttttt tcaatacgtc gataatggtt ttgctgcgga gtttttcaac ctgatggaag
      361 atgatgtgcc aacggcagac aagttcggca agctcggcgc aatgtttcgg cgcacgcagc
      421 cgctgattga cttcgcgcac gggttcgaca ccggcgaggt cgtgtccgtg gtggcgcggc
      481 aggatgtcgg acggtgtttt ggctttgccc aagtcgtgca gcagggcggc atagcgttcg
      541 ggcaggctca aacccatatc ggcggcgcgt tgcagcgtca tcagggtatg gatgccgctg
      601 tcgatttcgg gatggtagtc ggcgcgttgc ggcacgccga agagggcatt gacttcgggc
      661 agcaagactt tgagcgcgcc gcattcgcgc aacacttcaa tcattttgcg cggatttttt
      721 tccatcaaac ctttcgcaaa ctcctgccag acgcgttcgg caaccaatgc gtccgcttcg
      781 ccgttttcca ccatctgccg catcagcttt atggtttctt cggcgatttc aaacctgtaa
      841 cgcgcggcaa agcgggcagt acgcaggatg cggacggggt cttcggcgaa ggccggggaa
      901 acgtggcgca aaatgcctgc cgccaaatcc cgttgtccgc cgaaagggtc gataatcttg
      961 ccgtccgcat cttgcgccat cgcgttgatg gtcaggtcgc ggcgcatcaa gtcctgctcc
     1021 agcgtaacgt ctttgtcggc gtggaaactg aaaccgacgt aacctttggc ggttttgcgc
     1081 tcggtgcggg cgagggcgta ttcttcgtgc gtttcgggat ggagaaacac gggaaaatct
     1141 ttgccgaccg gctggaagcc ttgcgccagc atggtttgtg cgtctgcgcc gacgaccacc
     1201 caatcgcggt ctttgacggg caagcccaaa agataatcgc ggacggcgcc gccgacgagg
     1261 taagtctgca tatgcttcct attttaaagt tatcaacaat gccgtctgaa gcggcttcag
     1321 acggcattgt tccaaccggc ggttatgcta cgcctttttt ctccaagtaa ctttcgtaat
     1381 cgcccaagta gtgttcatat ccgcctttgc cgtccagttc gatgatttgg gttgccaagg
     1441 aggaaacgaa ctgacggtcg tgggagacaa aaatcagcgt gccgttgtat ttttccagtg
     1501 ccatgttcaa ggattcgatg ctttccatgt ccatatggtt ggtcggttcg tccatgacta
     1561 agacattggg tttcaacagc aacagtttgc cgtaaagcat acggcctttt tcaccaccgg
     1621 agagaacctt cacttttttc acgacatcgt tactgccgaa gagcaaacgc cccaaagtgc
     1681 cgcggatgac ttgttcgtcg tcgccttcct gcccccattg gcgcatccat tcgctcaggt
     1741 ccatatcgac gtcgaagtcg ttttcatggt cttgcggata gtagccgaca ctggcttttt
     1801 ccgcccattt gatggtgcct tcgtccggta acaggccgtc tgaatattcg gggttgtacg
     1861 cgccggccaa gagtttcagc agggtggatt tgcccgcgcc gttcgggccg atgatggcga
     1921 ggcgttgtcc cgcttcaagg atgaagttca ggtttttaaa caactgggtt tcaaagcgtt
     1981 tcgccagttt ttcaacttcc acagcctgac ggtgcagctt ggctttttca tcggcttcaa
     2041 aacggatata cgggttttga cgggtggaag gtttgacttc gaccatctcc gatttgattt
     2101 tgtcggcctg tttcagacgg ctggttgcct gacgggcttt ggatttgttg gcagagaagc
     2161 gggcgacgaa ctcttgcagc tcttgcagtt tctctttggc tttggcattg tctttcaggg
     2221 cgcgttcgcg cgattgggcg gaggcgagca tgtagtcgtc gtagttgccc ggatagatgg
     2281 tgatggtgtt gtagtccaaa tccgccatgt gagtgcagac ttcgttcaaa aagtggcggt
     2341 cgtggctgat gataatcatc gtggagtcgt attggttcaa cacgccttcc aaccagcgga
     2401 tggtattaat gtccaagtta ttggtcggtt cgtccaagag caatacatcc ggcttggaga
     2461 acagcgcctg cgccagcaat acgcgcagtt tgaagcccgg ggcgacttcc gccattttcg
     2521 cattgtgcaa atcttcggaa atgcccacgc cgctcaacag ttcggcggca cgcgcttcgg
     2581 cggtgtagcc gtcgtattcg gcgaacttgg cttccagttc ggcggctttc atgtagtcgt
     2641 cttcggtggc ttcgggattg gcgtaaatcg catcacgttc ggtcatcgcc gcccacattt
     2701 cggtatgccc catcatcacc acgtccagca cgcgcatgtc ttcgtaggca aactggtctt
     2761 ggcgcaattt acccaaacgc acgccgtttt caatcgccac ttcgccggcc gtctgttcca
     2821 aatcgccgcc gaggattttc atgaaggtgg atttgcctga gccgttcgcg ccgatcaaac
     2881 cgtagcggtt gccttcgccg aatttaacgg atacgttttc aaacagcggc ttcgcgccga
     2941 actgcatggt gatgccgttg gtagaaatca tgattggtaa agcctttatt gaacaagaga
     3001 ttaaattttt cgaattttcg gattgtaaca taaccgccct gccgtctgaa acagacgggc
     3061 aaattttcag acacttatag taaaatatcc gttttattat ttcagacaaa aggtttccaa
     3121 atgatagtcc ttcacggcat cccaaattgc gatacggtca aaaaagccaa aaaccggctt
     3181 gccggatacg gcttggagtt tgaatttcgg gattttaaaa aacaggtgcc ttccgaagcg
     3241 gaaatctgct cgtggctgga acaagtgcct ttggcaaccc tgctcaacaa acgcgggaca
     3301 agctggcgca aactcgatgc cgaaacacag caaaaagccc tgttctcgac agcggaggcc
     3361 gtcaaactga tgtccgaaat gccgagcctg atcaagcgtc ccgtattgga gtgcggcggc
     3421 aaggtttacg ccggcttcag cgaagaaacc tacgacggca tcttcaaccg gcaagccccg
     3481 tgcagacagg gataaaaacc gttacaatac ccgacttgaa tttcccgttc ccattctata
     3541 tcccgattta aaatatgttc cattccatcg aaaaatacag aacgcccgcc caagtccttt
     3601 tgggcctgat tgcattaacc ttcgtcggct tcggggtcag cacggtatcc catccgggtg
     3661 ccgactacat cgtccaagtg ggcgacgaaa aaatcagcga ccactccatc aacaacgcca
     3721 tacagaacga acaggcggac ggcggcggcc cttcgcgcga cgcggtgttc caatccctgc
     3781 tgcaacgcgc ctacctgaaa cagggcgcga agctgatggg catttcggtt tcttccgaac
     3841 aaatcaagca gattatcgtg gatgatccca atttccacga cgcaaacggc aaattcgacc
     3901 acgcgctttt aaaccgctac ctgtcgcaac gccatatgtc tgaagaccag tttgtcgaag
     3961 aaatccgcga tcagtttgcc ttgcagaatt tggtaaacct cgtccaaaac ggcgtattgg
     4021 tcggcgacgc gcaggcggaa cagctgatca ggctgacgca ggtcaaccgc accatccgtt
     4081 cgcacacttt caaccccgac gaattcatcg cccaagtcaa agtgtctgaa gccgatttgc
     4141 agaagtttta taacgcaaac aaaaaagact acctgcttcc caaagcggtc aaattggaat
     4201 atgtcgcctt gaatctgaaa gactttgcag acaaacagac cgtcagcgaa acagaagtga
     4261 aaaatgcgtt tgaagagcgc gtggcgcgtt tgccggcaaa tgaagccaaa ccttctttcg
     4321 agcaggaaaa agccgccgtc gaaaacgaat tgaaaatgaa aaaggcggtt accgacttca
     4381 ataaggcaaa agaaaagctg ggcgatgacg cgttcaacca tccttcctcg cttgccgaag
     4441 ccgccaaaaa cagcggtttg aaagtcgaaa cccaagaaac ttggctgagc aggcaggatg
     4501 cgcaaatgtc cggtatgccc gaaaacctga tcaatgccgt attcagcgac gacgtattga
     4561 agaaaaaaca caattccgaa gtgctgacca tcaacagcga aaccgcgtgg gtcgtccgcg
     4621 ccaaagaagt ccgcgaagag aaaaccctgc cgtttgccga agccaaagac gcggtacgtc
     4681 aggcttatat ccgtaccgaa gccgccaaac ttgccgaaaa caaggcaaaa gacgtgctta
     4741 cccaactgaa cggcggcaag gctgttgacg tgaaatggtc ggaagtgtcc gttttgggcg
     4801 cacagcaggc aaggcagtcc atgccgcccg aggcttatgc ggaactgctg aaagcaaaac
     4861 cggcaaacgg caaacccgcc tacgtcaggc tgatcggtct gccggcaccc gtgattgtcg
     4921 aagtacaggc tgtaaccccg ccggatgata tcgccgcaca gcttccgctt gcaaaacagg
     4981 ctttggcgca acagcagtct gccaatactt tcgacttgtt gatacgttat ttcaacggca
     5041 aaatcaaaca gaccaaagga gcgcaatcgg tcgacaacgg cgacggtcag taattgacac
     5101 ttttgttgac aaaataacgg tcggaatatt ccggccgttt tcccatacgg cggaatatga
     5161 tgagccacaa aaaacaagat gccttccaag gattgatcga cgcgctgaag gttttaccca
     5221 acgtcgggcc gaaatcggca cagcggatag cgtatcattt actccaacac aagcgcaaag
     5281 aggctgaaaa actggtggat gccttgcaga cggcattgaa gcaggtcgat cattgcgcga
     5341 tgtgcaacac gttttgcgaa ggcggattgt gcgatatttg tgccgatgaa acacgcgacg
     5401 ggcggcggct gatggtggtg catatgcctg ccgacgtgtc gaatatggaa gcggcaaact
     5461 gccacgacgg gctgtatttc gtcctgatgg ggcaaatcaa tacggcattg ggaatggacg
     5521 tatccgccat cgcattggac aggctggcgc aacggctggg cggcggggaa gtcgaagaaa
     5581 tcattattgc aaccgctttt accgcagaag gcaatgcgac ggcgtatgtc ctgtccgagt
     5641 tttttaaaaa cctgccttac aaagtcagca ggctgtcgca gggcattccc ttgggcggcg
     5701 aattggaata tgtcgatgcg ggaacgctgg cgcaggcggt gtacgaacgc cgcctgatta
     5761 aagaaggcgg ggcataacgc cgccaatgca aaatgccgtc tgaagccttc agacggcatt
     5821 tttccgggcg gtttacttgc agtttagccc ttgaatctgc aatgcgcctc cgttgccgac
     5881 tttgaacacg cactgcgtcg cgctgctgcc ggacacggta atgctgtcgc tgccgccgtt
     5941 caggctgata ggttccttaa cacccatacc gactgccgtt accgccagct tttgcgcgtc
     6001 ggcttcggaa taaggcgtgt tgttggcatc gattttgatt tctttggcgt aggcagccgc
     6061 agcggcggat aaggcgatgg cgcacaaagc aaaagcagtt cggatattca ttgttttcct
     6121 caagaaacgg tttttctgaa ggaaaggata gggcagtttg acgcgttgtg caagttttcc
     6181 gcccgctctg ccgccccgtc ccccgcactc gggcgttcgg acgctgccgg ggttttacgg
     6241 tacaataaac ttttgccgcc gcccccaaac cgccgtccgg ggcggcacgt ttgcagacat
     6301 ttttaaggta gcgttatgtt ttctctagag gcttggatag gcttgaggta tctcagggcg
     6361 aaaaagcgca acggctttat gtcgtttatc acgatggttt cgattgccgg aatcgccttg
     6421 ggcgtaaccg cgctgattgt cgtcttgtcg gttatgaacg gctttcagaa agaaatacgc
     6481 gggcagctcc tgaatgtcgc gccgcacgcc gaaatcggct atatcgacaa tacggatacg
     6541 gattggcgca acctgcttcg gtttaccgaa aaccgcaaag gtattttggc tgccgcgccc
     6601 tatgtttcca atcaggcatt gctggccaat gcgggcgaaa tcaggggcgt gcagatgcgc
     6661 ggcattttgc cgtctgaaga gcgcaaagtg gtggaatacg gcgacaaaat gccggcaggc
     6721 aaatttgaag acctgattcc gggcgagttt gacattatcc tcggtgtcgg cttggcggag
     6781 gctttggggg cggaagtcgg caataaagtt accgtcatca cgccggaggg caacgttacc
     6841 cctgccggag tcgtgccgcg cctgaagcag tttaccgtgg tcggtctggt taagacgggc
     6901 gtttacgaag tggacaactc attggcgatg acgcatatcc aagacgcgcg cgtactgtac
     6961 cgcttggata aggaagttgc ggggctgcgg ctgaaactcg ccgacccgca aaacgccccc
     7021 gccttgacgg caacactgat tccggaggcg caaagggata cggtttgggt gcgcgattgg
     7081 acgttcagca atcgaagcta ttttgaggcg gtcgagttgg aaaaacggat gatgttcatc
     7141 atcctgacgc tgattatcgc cgtggcggcg ttcaaccttg tctcttcctt ggtgatggcg
     7201 gttacggaaa agcaggcgga cattgcgatt ttgcggactt tgggtctttc ccctgccggc
     7261 gtgatgaaga tttttatggt gcagggcgcg ttttcaggct ttttcggcac gctggcgggt
     7321 gtggtctgcg gcgtgctttt gggttggaac gtcggcaggg tcgtggcatt ttttgaaaac
     7381 ctactcggtg tccaccttat caattcgcag gtttatttta tcgactacct gcccagcgat
     7441 gttgatatgg gcgacgttgc cctgattgcc tgcatttctt tgggactgtc tttcgttgcc
     7501 acgctctacc cgagccggcg cgcgtcaaaa acccaaccgg cggaggcttt gcgttatgag
     7561 tgagttgatt ttgaaatgcg aaggcgtggg caaacgctat cgggacggcg gtttggacgt
     7621 tcgggtgctg cacggcttgg atttggaaat ccgcgcgggg gaaagcactg gcatcatcgg
     7681 ttcttcgggc agcggaaaat cgacgctgct gcatattcta ggcgggctgg atatgccgtc
     7741 tgaaggcagg gtgctgctga tgggcgagga tttgcgtacc ttaaaccagc ggcgtttggg
     7801 cgatttacgt aaccgccatc tcggtttcgt gtaccagttc caccatcttc tgcctgaatt
     7861 ttcggcactg gaaaacgtga tgatgccttt gctgatcggc aaaaaaaacc gtgaagaggc
     7921 agcagaaacg gcaatggcga tgctggaaaa ggtcggactg aaacaccgtt cgacgcaccg
     7981 cgcgggcgaa ctgtccggcg gcgaacgcca gcgtgccgcc attgcgcgcg ccttggttac
     8041 gcaaccaaaa tgcctgcttg ccgacgaacc gaccggcaat ctcgaccgag caaacgcaag
     8101 gaatgttttg gatatgatgc tggaactgaa aacagaattg ggaacggggc tggtggtcgt
     8161 aacgcacgac gacgaactcg ccggccgctt cgggcgcgtg atggtcatga aagacggcag
     8221 cctgcacccc aaacagggcg caaacgccta aacagccgaa acaacaccgc gccgtctgaa
     8281 gccccacgcc ttcagacggc atttcgacaa ggaatacgat ggaacggcag acagatgaat
     8341 ttgcccaagc ggcggcaagg gcggcaatct ggtttttggc acgctatgcg ggttccagcg
     8401 acgaagtgct tgcaaactgc accgaacgcc tgtttcaggc attgcaaaac ggtcattcgt
     8461 ttatccgttt gagcggtggc gaggctgacg cgctgtcggc actcgcaccc gttgtcggaa
     8521 catccgccgc gcctttgatt ttggaaggca gaaggctgtt tttgggcagg atgtggcagt
     8581 tggaatacga tttggctgcc gagataaaac gcttggcggc agccgacacg tccgcccccg
     8641 acgcggcagg cgcgaggcaa aacctcgcaa aatggtttca aggcacgggc agcgaagggc
     8701 agcgcgatgc cgccgccttg gcactgttgc agtcttttat ggtgattacc ggcgggccgg
     8761 gaacgggcaa aacgacaacg gttgccaaac tgctggcgct gatttgcggt gaagacgaaa
     8821 atcttcccaa catcgcgctt gccgcaccga cgggcaaagc ggcggcacat atggcgcgcg
     8881 cgctgcaccg tgcaatcaac ggttttgacg cgccggaggc cgtccgccgc catttgctca
     8941 aactggaagg gcaaaccgtc caccgactgc tgaagctgcg cccgcccaaa atgcaggcgg
     9001 cgttcaaccc tgtttacccg ctgccgtttg acgtattggt tatcgatgaa gcctctatgc
     9061 tggatacggc attgatgctg caacttttaa aagcggtcaa aaccggcgcg cgcgtgattc
     9121 tgctggggga tgaaaaccag ctcccgtccg tcggaatcgg ggcggtgctg tccgttttgt
     9181 cgcaaaaaac cgttttggac ggagaaacgt ataaaagttt gaacctctac ctgcccgaac
     9241 acggatttcc ggtacacgaa gatgctcccg tgctggcgca aaacactgcc catctgtcgt
     9301 tcagccaccg cttcggagac aacagcggca ttggctgcct tgcccgtgcc gccgtatcgg
     9361 gcgatgaggg ggcgtgggca ttgtttgacc ggtttccgga cgaactggaa cattcggaat
     9421 gcagtccgaa cgctcgagtc gaaaggttgt accgggcgca caaagcctat tggcaggcgg
     9481 taaaagacgg caatatcgaa gccgcatacg caggcatctc ggatatcgtc gtcctcgcgg
     9541 cgtggcggca ggatgcggaa gacttcaacg aagcctattg cagccatgta cgccgcaaaa
     9601 tgaacatacc ggaacatcag gcctatttcg ccggagaacc gattatgatc agacagaacg
     9661 actacgcgct tgaactgttc aacggcgaca tcggattgat tatggaagat gtcggacggc
     9721 agggcggcct tgccgcctat tttgccgatg cggacggatt taaaaaggta gcggtaagct
     9781 gcctgcccga tttcgaaccc gcattcgcca tgaccgtcca caaaagccaa ggttcggaat
     9841 atcgggaagt ctggctgctg ccgccttccg ccgcaccttc ggacgaaggg gacgacgcgc
     9901 tttcgggctt gagcagaggg ctgctttaca cagccattac ccgcgcgagg gaaaagttcg
     9961 tatttttcgg cggggaagaa gccttccggc aagccgcctc caccgtcaaa gcgcgtcaga
    10021 cggcattggg cagtatgctt gggcggatat tttcacaagg ataatccgca cgaatgccgc
    10081 gccgccgccc cttatgcctt tttcaaacgg tacaggaaag tagtttccct gtttcgggca
    10141 aaagccagcg gattgttcgg atcggcagct tttttgtgaa ccggtttggt tttcaaaaca
    10201 tccgcaacgc gcaaacccgc ctgagcaaat gccttatcca gaaaatcgga cggacgcagg
    10261 tgcagatgtt cggcaagatc ggaaatgatc aggcggattt ctccgtcggg attcagatgt
    10321 ttcggcgcat cccgcaaaaa cgcagccagc atcgcagatt cggggtcgta taacgcggat
    10381 tcgacggcgg aagtcggctt ggcgggaagc cagggtggat tgcagacaat cagatcggca
    10441 aacccttcgg gaaacagatc ggtttcccgt atctcaacct gtttttcaaa gcccaaacgg
    10501 gcaatattgg cacgggcgca ggcgacggct ttcggattgg tatccgtgcc gatgacggaa
    10561 ggaatgccct gtttcgccaa aatggcggca agcacgcccg agcccgtccc gatatcgaat
    10621 gccgtctgaa aacccgttga cggcgcatgg gcgagcaggt cgaggtattc gccgcgcaac
    10681 ggcgagaata cgccgaaagg aacgtgtatg ctgccgccca gctgcggaac ggcaacccct
    10741 ttcttatgcc actcgtgcgc acccataaac cccagcagca gattgagcgg caggaaaaac
    10801 ggtttgccgt ccgcctctcc gtacacgtcg agcaaagcgg agcgtatatc gggcgcgcgt
    10861 ttgttgtcca acacaaaacc ggggcggatt tcaacggcaa gcatattcag aatacggctc
    10921 tgctgcgcct gcttcatacg gtgggcgtgg aaaagggcgg cgatatccgc atcggaacgg
    10981 acggcggcag gtttgcggac cttcttcttc attgcggaaa gcacctgttt ggcattgtgg
    11041 aaatcgccct gccagacagt tgcaatattt tgataggcag ccttcaaaat gccgtttgca
    11101 ccactttcgc ggacataatg ccaacctttg ggcggctttt gcagactttc gttgcgccat
    11161 tcgaacccgt tatcgggaaa aataaaagaa gacatggaat acctgcgtca tgttttgaaa
    11221 atagggcggc agaaccgcaa accatacgga tggtacagca aggagcggca acacagaaca
    11281 gttttttgtt cccgccttgt ctttccaagc ccatgccgtc tgaagccgga atgtttcaga
    11341 cggcatcgca tcaaactcca taaataaacc acatatgctt gaaataatac cttcaacccc
    11401 aatgtacgcg aaaatcggca atctgtcaga cacaagagag tacctatgac acaaaaagaa
    11461 aagcattttg aggaatatgc cgccttggca acccttcctt tgcgggatgt cgtcgtttac
    11521 ccgcatatgg ttctgccgct gtttgtcggc aggccgaaat ccatcgccgc actggaaaac
    11581 gccattaccc gcgaggagcc ggttttcctg ttggcgcaaa ccgatgcggc gctagaagaa
    11641 ccggttgccg ccgacctgta tcagaccggt acggtcgcac aagtcctgca agtgttgaaa
    11701 ctacccgacg gcacggtaaa agtattggtc gaagggctgt atcgcggacg tgttctgacc
    11761 attgaagaca cgggcggtct gttcgtttcc catatagaga cggtcgtgga agaagacacg
    11821 ggcggcaata ccgacctcga agccgtgcgc cgcaccctgt tggcgcagtt tgaacaatac
    11881 gccaaactca ataaaaaaat ccccgccgaa attatcggca gcattaacgg cattgccgaa
    11941 aacagccggc taaccgatac ggtcgcagcg catttgcagt tgaaactggc gcaacgccaa
    12001 cagattttgg aaattcccga aatcggcaaa cggatggaat tcctgctggc acagctggaa
    12061 tccgaactcg acattatgca ggccgaaaaa cgcatacgcg gacgcgtcaa acgccaaatg
    12121 gaaaaatccc agcgcgaata ttatctgaac gaacagatta aagcgataca caaagaattg
    12181 ggcgaagaag acgaaaacgg cgaactggat gccttggaag caggcatcaa aaaggcgggt
    12241 atgagtaaag aagcggaaga aaaatgcctg tccgaactga aaaaactcaa aatgatgccg
    12301 ccgatgtctg ccgaatccac cgtcgtacgc aactacatcg acaccctgct cgaactgccg
    12361 tggaagaaaa aatcccgcgt cagcaaagac atcgccaaag ccggactggt gctggatgcc
    12421 gaccactacg gcctggaaaa agtcaaagaa cggattttgg aatacctcgc cgtccaaaaa
    12481 cgtatggaca aactcaaagg cccgattctg tgcctggtcg gccctccagg cgtgggcaaa
    12541 acctctttgg gcgaatccat cgccaaagca acggggcgga aatatgtccg catggctttg
    12601 ggcggcgtgc gcgacgaaag cgaaatccgg ggacaccgcc gcacctatat cggctccatg
    12661 cctggtaaga ttctgcagaa tatggcaaaa gccggcgtaa aaaacccatt gttcctgctc
    12721 gacgaaatcg acaaattggg tagcgacttc cggggcgatc ccgccagcgc gttgctcgaa
    12781 gtgcttgatc ccgagcaaaa caacaagttc gccgatcatt atgccgaagt ggattacgat
    12841 ttgagcgatg tgatgtttat tgcgacttca aacagcctga atatcccgac cccgctgctc
    12901 gaccgtatgg aaatcatccg tctgtccggc tacaccgaag acgaaaaaat caatatcgcg
    12961 atgcagtacc tcgtgccgaa gcaaatgaag cgcaacggcg tgaaagaagg ggaattggca
    13021 atcgaagaaa gcgcggtgcg cgatattatc cgttactaca cccgcgaggc gggcgtgcgt
    13081 tcgctcgacc gcgagattgc caaaatctgc cgcaaggtgg tgatgcagat taccttggac
    13141 gaagataaga agaggttgtc tgaaaccaag aaaaccagca aagccaaacc taaagcggtt
    13201 aaagtaaatg agaaaaatct gcacgactat ttgggtgtgc gccgcttcga ttacggcgtt
    13261 gccgaaagcg aaaaccgtat cggacaagta accggtctgg cgtggacaga agtcggcggc
    13321 gaattgctga ccgtcgaagc cgcagcattg ccgggtaagg gcgtgattca gtgcaccggc
    13381 cagttgggcg atgtgatgaa ggaatccgtg tctgcagcgt ggtcggttgt ccgctcccgc
    13441 gcggaatcag tgggcttggc tcctgatttt tacgagaaaa aagacatcca tgtccatgtt
    13501 cccgaaggtg cgacgccgaa agacggtccg agtgcgggca ttgccatgac tttggcgatg
    13561 gtgtctgcat tcaccaaaat cccggtgtgc gccgatgttg ccatgacggg cgaaattacc
    13621 ctgcgcggcg aagtcctgcc gattggcggt ctgaaggaaa aactgttggc tgcgcttcgc
    13681 ggcggcatca aacacgtttt aattccaaaa gacaacctca aagacttgga agaaatccct
    13741 gaaaacgtga aaaccggcct gaccatccat ccggtcaaat ggatagacga ggtattggct
    13801 ctgggtttgg aaagccagcc tgagccttgg gcagaacctt ctggtgcgga agcggcggcg
    13861 gaatccgctt caaaaccaaa accccgcagc agggcaacca aacattgaaa cgcaggaaat
    13921 gtgttgtaaa aatgcggttt cgtcctgaaa gcctgtcaaa tagggtgatt ccgtattttt
    13981 tgcttgacac ggcaatttca gaattgctat aaagcgaaag ttgctcaagc agtacaaacc
    14041 ccgagtattc gggcatttca tcatttttta agacaggaag ggactgattg tgaacaagtc
    14101 tgaattgatc gaagcgattg ctcaagaagc cgacatttcc aaagccgccg cacaaaaagc
    14161 tttggatgcc actaccaatg cagtaaccac cgccctgaaa caaggcgaca ccgttacttt
    14221 ggtcggtttc ggtactttct acgtgggcga acgtgcggaa cgccaaggcc gcaaccccaa
    14281 aaccggcgag cctctgacca ttgccgccgc caaaacgcct aaattccgcg ccggcaaagc
    14341 tttgaaagac gcactgtaag ccgtttttta tgaaaaaagc cgattcttta aagaatcggc
    14401 ttttttatcg gtccacatta ttctgatttc aaatcggtaa cacactgctt gtctcgcgct
    14461 tcaaaggcat ttgcgccgcc gagcaggtca agctgttctt gtgcgccgag tttgccgaag
    14521 gatctaatct gtttctcgct caatctgtcc aaaggctgct cccacataca tttgcagtag
    14581 tcgacggcga ggcgggtatt gtttgaatct aaaccgcggg cccgcaaatc gttttgccat
    14641 ttttcggcaa acggaatatt cttcacgcaa gactcgacaa ttttctgttt tgcctgcggt
    14701 ttggacatcg cgcattggga gagcagggcg gttaaagcga gcaacgccaa aatgacccac
    14761 gcccaaatgc ggatggtgcg gattttggct tttgcttttt tgcgcgcggc aacctgctct
    14821 ttcgtcagca tttcgtgttt cggctcagtc atgcaggctt tccatgcgga tcatggtaat
    14881 cggtttttcc acgcaatcca gtgcttcgat ggctgcgatt gccgacttga tgtgtttttc
    14941 gaccgtgctg tgggtcagaa tcacgatttc ggcagtggtc tgatcaatca cgcctttttg
    15001 gattaaggct tcgatggaca cgttttcttg tgccaacagc gcggcgattt gccccagtgt
    15061 gcccggttcg tctttggctt ggacgcgcag gtagtagctg ctggtaattt cgtccatagg
    15121 caggatggtt tgcgcttgga cttgcgcggg ttggaatgct agatgtggta cgcggtgggc
    15181 ggtatcggct tcaaccaggc gggcgatgtc gatgatatcg gcaaccacgg cggaagcggt
    15241 cggcaatgcg cccgcgcccg cgccgtaata taaggtttcg ccaaccatat cggcgttgac
    15301 gcgcacggcg ttcatcacgc cgttgacgtt tgccaagagg cggctttcgg gaatcagggt
    15361 agggtggacg cgcagctcga tgcctttgcc ggttttgcgg gtaatgccca acagtttgat
    15421 gcgatagcca agttcttcgg cgtatttgat gtcgcggctg tcgagtttgc tgatgccttc
    15481 gaggtagcag gcggaaaagt tcatcggcgt gccgaatgcc agtgcgctca tgatggtgat
    15541 tttatggccc gcatcgttgc cttcgatgtc gaaggtcgga tcggcttcgg cataacccaa
    15601 tgcctgcgct tctttcagta catcggcaaa cgcgctgcct ttttcgcgca tttcggagag
    15661 gatgaagttg ctggtgccgt taataatgcc ggcgatggat ttaatcctgt ttgccgccaa
    15721 accttcgcgc agggctttga tgattgggat accgcccgct actgccgctt caaattggac
    15781 gatgacgttt tgtttttccg ccagcgggaa gatttcgttg ccgtattcgg cgagcagttt
    15841 tttgttggcg gtaacgatgt gtttgccgtt ttcaatggct ttcaacaccg catctttggc
    15901 aatgccggta ccgccgaaca attcgacgac gacatcgacg tcttcacgtg cgaccagttc
    15961 gaacggatct ttgacaaagg ctgcggacgg gcaggtttgt cgggcttttt cttcactcaa
    16021 atcgcacacg gcagaaatac ggatttcgcg ccccaagcgg cgggaaattt cctccgcgtt
    16081 gtcccgcaac acggcagccg taccgccgcc gaccgtaccc aaacctaaaa gaccgatgtt
    16141 tactggcttc attgtgtctc cttgtaagcc gactgaaatg taaatattga aagacgaaaa
    16201 tatccgctgc cgatataatt gtgccgcact ttgaatcaaa tgccgtctga aatcggcagg
    16261 cgggtcagat gaaatctgcc aatcctacat gaatttgtct gattttgcac ccctttcggt
    16321 gtagatgatg cggcgacggg gtaaaaaaat gttgtttgaa gaaaatccga tagacggaca
    16381 gtttgcggaa tatgaatgcg gtgcgggcgg aatccggctg gcgaggcaaa gtttccataa
    16441 acccgtgctt gtacataagg attcggtctg cctgtcgcaa tgccgaacct tgtccgatct
    16501 gactccggaa aacctgttgt ccgacatcaa acctgttgac tatccggaaa tattgattat
    16561 cgggacgggc gcggctcagg agtttatcca tcccaaaatc atggcggatt tttcccgaat
    16621 cggaatcggc gtggaatgta tgaataccga ttcggcattc aggacattgg ttttcctgca
    16681 ctcggaaggg cgcagggctt gggcttggct tcagccgtaa atttcccgtt tcagacggca
    16741 tcggcactga ctttcaggta aaatacgggc ttttcccgcc cgacgatgtt tccgttatga
    16801 ttgaaatcaa aaacctcacc ctgcaacgcg gtttgaaagt cctgctcgac aaagccaacg
    16861 ctaccgtcaa tcccggtcag cgcgtcggtt tgatcggcaa aaacggaacg ggcaaatcga
    16921 gcctgtttgc cttaatcaag ggtaaaatca ctcaggacgg cggcgatgtc tcgattccga
    16981 aaaactggcg gctcgcttcc gtttcccaag aaacgcccga tttggatatt tccgctttgg
    17041 attacgtttt gcagggcgat gccgagttgc aggcttttca gacggcattg aggcaggcgg
    17101 aagcgcaaaa tgacggcatg aagcaggcgg aatatcacgc caaattggaa gaaatcgacg
    17161 cttataccgc gccggcccgt gcagcgaaat tgttgaacgg gctgggtttt tcgcaagaag
    17221 aacacagccg tcccgtcaaa tccttttccg gcggctggcg tatgcgcctg aatcttgcgc
    17281 aagccctgat ttgccgcgcc gatttgctct tgcttgacga accgaccaac cacttggatt
    17341 tggaaaccgt cttgtggctg gaaaaccacc ttgcctcttt accctgcacg caaatcatca
    17401 tttcccacga ccgcgatttc ctcaatgcgg caaccaccca aaccatcgaa ttgtcccagc
    17461 aaaagctcac gcaatacggc ggcaattacg atttttacca aaacgaacgc gcgcagcgtc
    17521 tcgcgcaaca acaagctgcc tatgtcaaac agcaggcgca aatcaaacat ctgcaatcct
    17581 ttatcgaccg cttcaaagcc aaagccacca aagccgttca agcgcaaagc cgcatgaagg
    17641 ctttggcaaa gctagaacgc gttgcgcccg cgcatctgga cagcgagttt tcctttgagt
    17701 tttaccatcc cgaccatctg cccaatcctt tgttaaagct agaacacgct gatttgggtt
    17761 acgaaggcaa aaccgttctg catgacatta ccctgtcgct ggaaagcggc gcacgctacg
    17821 ggctattggg tgtcaacggc agcggcaaat ccacctttat caaagccttg gcaggcaaaa
    17881 tcgatttgct ttccggcagc atcatccgtt ccgaaaaact caatatcggc tattttgccc
    17941 aacaccaact cgataccatc cgcgccgacc aaaaccctgt ttggcatatt cagcagcttt
    18001 cgcccgaagt gcgcgaacag gaaatccgaa atttcctcgg cggcttcaat tttgttggcg
    18061 atatggcgtt acagaaaacc gaaccttttt ccggcggaga aaaagcccgc cttgcccttg
    18121 ccatgattat ctggcaaaag ccgaacctgt tgctgcttga cgaaccgacc aaccatttgg
    18181 acttggatat gcgccacgcc ttaacgctcg cgttgcaaag tttccaaggc gcattaatcg
    18241 ccgtatcgca cgaccgcagc ctgcttgaag cgactaccga cagcttcctc ctgatcgaca
    18301 aaggccgtct gaagaacttc gacggcgatt tgaacgatta ccgccaatgg cgtttggcac
    18361 aggaaaacgc cgccgccgca cccgcagcat ccgcacaaag ccaaagccgc aaagacacca
    18421 agcgcatcga agcgcaaatc cgtcaggaaa aagcccgacg cggcaagccg atacagcaga
    18481 aaatagaccg tgccgaaaaa gaaatggcgc agctttccga aattcagacg gcatgtgaag
    18541 catttttagc acaagaagac gcttaccttg aagcaaacaa agaaaaattg caaaatacct
    18601 tatccctgct gacaaaagtc aaaacacaac ttgcccaaat cgaagaggtt tggctggctt
    18661 gtcaagaaga attggaacgg attgaaactg aaatcgagaa acagtttgcc gagcgataaa
    18721 gaagcgcgga cagggtgctt atctttgcct gccaataacg gtataattcg ggcgttatca
    18781 ccgcctttta ccggtataaa catcagactt tttgccgcct tgggtctcct gtccctttcc
    18841 ggcgcggcgg cgcaagcctc cgtataccat tgcaactcaa atggcaaaag cgtatataca
    18901 tcagccccag caggatcgtg cgccgatgcg gatttaccta aaatcagcag ccatcaggga
    18961 ggcggatacc gcctgaaaat taaaaaactc agtcaagaag ccaaaataca catagaaagt
    19021 aaaaagaaaa acaaaaaacc tgccgggaaa aagaacagac aggctgccaa agccccgaaa
    19081 gaaaatcaaa agcaaacaac cgaaaagaaa agcccgtaaa acgccaagaa agcattacaa
    19141 aaaataccaa aaaatcaaat aattatccga gcatcaagca cattatgaaa tcaaaactcc
    19201 ccttaatcct aatcaacttt tccctgattt caagcccatt gggtgcgaat gcggccaaaa
    19261 tctacacctg cacaatcaac ggagaaaccg tttacaccac caagccgtcc aaaagctgcc
    19321 tctcaaccga tttgccccca atcggcaact acagcagcga acgctatatc ccgccccaaa
    19381 catccgaacc gacaccatca ccgtcaaacg gcggacaggc tgtcaaatat aaagccccgg
    19441 tcaaaacagt atccaagccg gcaaaatcca atacgccccc gcctcaacaa gcacctgtaa
    19501 acaacagcag acgctccatt ctcgaagcag aattgagcaa cgaacgcaaa gccctgactg
    19561 aagcccaaaa aatgttatca caagcacgtc tggcaaaggg cggcaacatc aaccatcaag
    19621 aaataaatgc attacaaagc aatgtattgg acaggcagca aaatatccaa gcactgcaaa
    19681 gagaattggg acgtatgtaa agccgtgttt tcaaatcgac cgttccaagg atttgacaga
    19741 agaaatgatg aaaaagcagc ggcatatcct gtgtgtcggc tgcctgtata ttatattgaa
    19801 ggtcaacgta tttcccaata ccggctggat ggaagcgtcg aaagggatgc agggcatatc
    19861 gttcagtacc ttcggatacg cggatgctcc gggctataca ttcagccgca ttgtccatac
    19921 tttaattttc ctacttggaa agaccacagg gacacgtaca actgtttccg atagttcgca
    19981 taatgtatat agagtgctgg tcataatttt gacttccggc aactgattcg tcagcatctc
    20041 tgctgtttcg acagttttca ccctaagcat aatcatcgta ttcaagttac caccaatttg
    20101 acctgcttta gctggggaac caattttggc ttctacgtct ttccatgttt gtgtataaac
    20161 cgtcacttgg tagccggcac caccggcctt attcaataac ggaatgaatt cgtcaccgat
    20221 taattcattg aattcgtctg catgaattga gacttttctt ttctgagttt ttccactctg
    20281 tccaactcca tggccgaatt tatagatctg acccgcaaga gaggttaagt cagcaaacat
    20341 cgcattacca acggctgctg caacttcggc atcagatagg gaatccaatc caatatagac
    20401 gacggcattc gattccatga ctttgcgcca gtccataatg attcttggat ctgacaaatc
    20461 ctctgtatcc gggctgataa gcttggcaac ttcgccagtc gtcagttttt ccagcaatgg
    20521 atacagggag ctgactagct tctcaaaata ggatctgtca ttggacaaaa taccacccaa
    20581 tgcgtcaata agaggttcgt tatgcttttt ggccttcaag aaagaaccga gcttcattgt
    20641 gtccaagttg cgcccagttt tgactgcgct ttttgcttca gactcgggca tatcaaaatt
    20701 gtcgaagtct ttctcccaac cagggtggtg tttctctaat atctttttaa agtaatcccc
    20761 tgcaagttca tctacatttg cagcagactt atagatattt tcatagcttg gtttaatgga
    20821 tagaccttcc atggttttcg ttaaaacgtt gacgaaacgc catacaaaat ctcggaaagc
    20881 agcagactga ccttcagaag gcagttggtt tgcaatcctt gtcgccacct cggtaatacg
    20941 accataatca ccgatagggt tgtaccggca actgtactcg gggaaaccca aatggaacat
    21001 ataaaatggc ctgtttgatt tttgcgattc gacatacatt ctagtcatca ggtcagcatc
    21061 accctttgga tcaaatgcga tcgtaacatc cccccgccga atatcctgaa caatcatggt
    21121 ttcgcatagt cgggttttac caacgcgggt tgtgcccaat acaaccttgt ggccaacacg
    21181 ctccctcaaa tcttcccaaa tatcatcttc ctcaatttca atgccatgta attcaggaga
    21241 acctccgacc ggaggtaagg gagcaaccgg attccaccat gccgatttcc gagttatacg
    21301 agaaatccag tctgtaccca ttgtctcaat ctcatgtttt cgcgctccct tatagagttt
    21361 tgaggggtta cgaagtccgt cgttctcagg ctggcgaacc ataaaaagac gttgggtatg
    21421 tgtctgtgtc catctaaatc cttttccaag aaaaagagct ttatcagaag aaggaatttc
    21481 atcagcagta agggcataat agggcaactt ccgaatattt ttacggaaac gcataatgcg
    21541 taatgctgat agcgcgcgtt tgactgaaac caacgaaaaa atagctaaac tgccataagc
    21601 aattgtcgga ggtacgggca aatgtgctac tgaaaccgct gtggtggagt atgcggctac
    21661 actgatccac tcatagggtg atcgataaac gttttcaggg aagtgggcac tcatttttgt
    21721 tccattacta ataagtcgga ttcatctaat ttaatatcgg tattattctg aggtcttgaa
    21781 cctggctgta tgatatgttt gatattatgt tcagggataa ggtagcaata gaagagtctg
    21841 cgattgctac catttgtagt tagaacccta tgcaatgagc tttttttcgt tcttgcatgc
    21901 aacctcaaag cctttttgtg taagagggcc tagactttcc gtatcagatt tattgaacac
    21961 gccgccggcg taatctcgaa atacggctgg cgtaaccaaa agcatcccct gttcaataaa
    22021 gtggactgtt gcaccacttc tattgacagc tatgctgccg tctcctaatc catctgccag
    22081 ccatcgtaaa aactgcattc cacggtctct ggcctccata tgtttggctt tgactgaatc
    22141 ctcgactatt tcggtcgact ctgtttctga ttgctttttc aaattacgtt cagaaactat
    22201 ggcttcgagt tcggccatat tttttgcccc acttgcaagg ctatctaaac cttcagagtc
    22261 atccatgact tgaactgggc gtggactaga tgtaggttct cttagtctta gctgctccag
    22321 ttcacgcata ccattttgtg ctgattcaga ttcaggcaat ggaactgctt cagaatctat
    22381 ttgatgagtt actgtctcag ttgttttaat ctgtgcacat tcaggttcgg gttcagctgt
    22441 ttttccaata ggtttacgat tgtctgcaaa aaggtcggca agtttcttac ggcttgcttt
    22501 ggttcctgat ttaattgttt ttggtgaatt atctgtctca gtttctttaa cctcagcgcg
    22561 aggtttgtct tctgtctcag ttttaacttc tactcttata ttttcttgaa ccaggttaac
    22621 ggcattttct gctgtctcag cttctgagtt ggttgattct gtttgttctg aatcagcttc
    22681 tatcaggtta aagttttcaa ttaagttatc aattgtgctg taacccagtt ctttagcttt
    22741 ctctgactcg ctagatttat ttttcgctgg agcagcagta ttcttactgt tgtcaaaagc
    22801 cgtgttagct gtctcagttt gatttttttc aggtagggga ggttgttctg ctatcaccgg
    22861 ttgaatagac aggaagtcca ttttgtcctt ggtcatggtg actgagctat cttcggcatc
    22921 ttgtgtctga gttgacactt tatcctctga tactacaacg tctggtattt ccttcttggc
    22981 ggaaacttct gaaagattac ctaagaactc agtcggataa tctccatccg gatacaaggt
    23041 tttggcattg aaacaaagta cactgaagat attacggatc ttttcaccgt ccatacgggt
    23101 aaactcaacc tctatatggt taattgccct gtgaggatcg ttttccggcg gaatgacggc
    23161 acggtactcg aataatgtgt caaatatcct ttggttgtca gctggaaatg agcctgcagc
    23221 aggatgttga tttttcctca aatactgccg gatgtagtca ggtacatttt ttgacatcaa
    23281 atagatgcga tcactcttac ggaacaggtc accaccggca gttgttgcga tgctgaaatg
    23341 gactcctcta tcggataaca tttccttcaa tgtttccata atggtttcaa tgaatggttt
    23401 acgcttggcg gtagagaagc gttgacggct gccgcttcgt aaatcccttg aaactgaggt
    23461 catatcggca ttttttatta actgctcaag aggactgcca tcctttttcc cactcagata
    23521 atttctcaaa gtaatcatta aatttggatc cgttgtggca agccattgtc tgacatgtga
    23581 aggaaccaaa gcttgaaaga atgtccaggc gatttcggca tgtgtactgt aagcagactt
    23641 tgcatcagga aattcgacac gataataaag cttgccactt tcagccatgg agcctgtatc
    23701 aggcaaccat aataattttt cgagactgat agctgaatca aacagttcaa cctgaaatcc
    23761 tgtcacagtt ttaccaacgt cgtgaagaag ggcagcacac atgattccat aacgccaaac
    23821 ggcggttttc ttcgctattt cctcggtttt ggcatttggg ggccatgatt gcgatgtaga
    23881 tgccacaaga gccagtgcag ctacatcgag tgtatggcgg actaaaccac catcacctgc
    23941 atggtgatga gactcactgg cgggaagcct ttgaacaaag gctatatatt tttccaaaaa
    24001 gggaagggca tccttactcc agttttcatc cgataaccct aaattcatct taataccgga
    24061 tatttgaggg cttaagtcga gcacctgaat cagttcatgc gcgtttaaaa tgcgaaaccg
    24121 atcatttact atgttctgat tgatatgggc aacctcatca ttatgtgaca cggaatctgt
    24181 actttttctc gtatttatca ggtatagcat tgagccgctg acaagcatta gtgaggatgc
    24241 aatagtgaga aggcttgttt tcatgataat ttaagcaaag attttatcca catggtgact
    24301 ttttcctttt ttgcatgtgg aatatcagca atcaattgat taggttgcgt agtatgtcta
    24361 atacaaaatt cttcaaagct ttcttcaagt gaattccagt attcagaacc gtattgagcc
    24421 attagaaata agatttcaaa gtcatcggca agtgcttctg caaaagtcgt gttatcaata
    24481 tctatcggca gtggctctag aagtttctct agtttttcga taaggaaggt tgccgtattt
    24541 tcatcgtcat ccagcgtcat catttccaga taaccggtag cacgctgtat ccaaaacaac
    24601 aatacgcaag accagttttt atcgatattc gtaaaccgat ttatgacact aagaagcttg
    24661 ggaagatctg tttcattcat tttgattaaa aagacagggt tgttaaacta ttcatataat
    24721 aaaaaagtta ccatgtcaaa aatcaaagta aaaaaggtta ttttaattag aattgtcaca
    24781 aaataagatt tataaggtat aatgatgccc cttttaatag gcaaaggcct gcatttttac
    24841 atgcaggcct ttgttttacc tttaactttt atcctttccc cttaagcctt ttcctttaac
    24901 cattaaggct gttgctttac cgttacagct tttaaaaaaa ggccggctgc gatttttttt
    24961 agcagacggc cttttggctt ttggataaga gcctttaccc ttatcatatt ccttttatat
    25021 caatatgtta tgaattggta atttcataaa atataagcgc aacttttaat tataccatat
    25081 ctatttttaa agaaacattt ctaaaaaatt tagttaattt cgtttgattt aacaaaaatt
    25141 gtcgaatcga gataaaaatt agtcatatat acaacgacag taaagacatg agattaaggg
    25201 ccatactttt acagttcgtc gtaccaggtg ttttggtact cacatctaca agagcagttg
    25261 ctttttgtta taaggaagcc ggcagcaaat atggcatcga tcctgccatg ttgcaatcga
    25321 tcgcattaac cgagagtgct ttcagaccga atattgaaag tcatacagct gatattggct
    25381 tgatgggtat caaccgttca tggctacccg tactccacaa gaaatttggt ttaacagagg
    25441 cggatgtatg gaatccatgc acaaatgtac atattggtgc atggatattg gcaaatagtt
    25501 atcggcaata tgggaaatca tgggaagcag ttggtgctta taacgccgca tgtaccaaac
    25561 ttaaaggccg ggcatgctat cgcgcaagaa tgacttacgc aaataaggta tatcaaaatt
    25621 ggaaacgatt aaaaacacgc tcttcgtagc ttccacaatg attctgacag cctgtgccgc
    25681 cccaaaacag gaagtggccg agcttcctca agacgcaact tatacagtgg aacaaaataa
    25741 gctgggcgaa tttgtcttct gccgcaatga agagtgttca ggatttgagc gcaaggtttt
    25801 ggcggaacaa ccggtttatg ctcaaacaca aaccaatctt caaaaagagg agatcagggt
    25861 gtttttcaat ttaggcagtt cacacctcgg tgcggagggc gtgaaaacgc tcaaacgtgt
    25921 tctgcctaaa ttgaaaaata ctcagacggt ttatttacgc ggctgggctg acagtatcgg
    25981 tggtcgtaat actgcaatta atagacgttt ggctaaacaa cgggctgcag taataggtaa
    26041 atggctgagg aaacataatg taaccgcaaa aataagaaca gcaagttccc ctgcctgctg
    26101 caatagtgat gacacgcgaa ctgttgtaat cacttggtaa agaatgatcg gacgcaaaag
    26161 cttggcgata caagctctat ttatgaggtt ctttatgaaa atcaaacaaa ctacagtagc
    26221 aaaatatgca gccgtgtttg catttgcttt tgccgttaac ttggcaatgg ccgctacaac
    26281 cggcgctgaa tttaaaggct tggccgacat ggtatcaggc tggtctgaag gctatctggg
    26341 tatgtctttg ggcttgactg cattcttgat cggtttggcc gtaggtctga tgaaacaaac
    26401 cgtcatgcca tgtatcgtcg gcttaggtgt tggcctggct gcaacattgg gcccgggcat
    26461 cattaaaggt atgtttacct tgattattta atcaaactag ataagagaag tgatgagaga
    26521 ttttcatgtg ccggtatcga tagacgatcc caaaaaagca tttatttggg acttggacgt
    26581 ttttatatta tttatgtccg gcttcggtat aggtattgtc tctcgtcact ttttcatttc
    26641 catgtcaatt agccttttca ttgcatggag gtggggcaag cttaagtctg gtaagcatca
    26701 atggtttttt ttgcatgttc tttactggta cttacccatt aatgaaaaaa acaaacggat
    26761 acctgaaaca aataaacggg agtttctaaa ttgaagactg aaaatatgtt gagtgaattg
    26821 gcgataagaa ccggcataaa ggtctttttt tggatacttt tgggaatgtc tatagctgtc
    26881 aatctatttt tgtccgcgac tctgctcttc aaaggtacga ttgttcaaac gacgctgact
    26941 ccgccggaaa tccggcggtc aatgactgta tcaaacatcg ctttttctaa agaatatctt
    27001 gaagaaatgg ctccctatac tgcctatctt ctcttaaatg taacccctaa aacagttgat
    27061 tatcagaata gtcaattatt gaagattgtt tcacctgatt ataaagatgc cctagagagg
    27121 gagctgtctt taaatgcctt atgggttaag aaaaataatg tcagcaccac ttttagcgcg
    27181 acagaagcaa cggcagacat ttcagacaat acagtttcca ttcgtggggt ttttgaggtt
    27241 aaacgcaaca atactctagt agagcaaaaa gaccgcgagc tacttatttc tttcaaaaat
    27301 aattatggaa cactgcaatt attatcgatt aaagaagttc aaaagcgtgt aactaaagca
    27361 gaacccgcag gtcaggaaaa atccgaagtt gaaaccgagg atgtagaaat cagtaaaacg
    27421 gcaacagaat ttaaaggcgg taattgaggt aattgatatg tgttcactgt taaaaaaaat
    27481 tgtcttttct gccttgttgg tagctggacc cacgactagt tttgcagcgc aacgagtgcc
    27541 ggcaacagaa aatacacctc acgttgtttc aataagtaaa cggaacttga gcagaatcgc
    27601 cattgaaggc ggtcgaattt catcatggaa atttatggag ggtgatcttg agcttcagaa
    27661 agatacaaca acaggccaac tatttgtccg tagtttgacc tccagcccaa caaatctttt
    27721 cgttgtaagc gaagaaggta agacctactt gctggttctt aaacctacat caaaacaggg
    27781 tgacaacatc gtaatcgatg tggctggagc taatcgccat gaagcagcag tattggcttc
    27841 acgaaccggt ccaatgcctg taacgatgaa tagtacagaa tatgtacgga ccatcaaaaa
    27901 aatgatgacc tctatgatgc gtggcactac cggcgatatg ggcatcaatc atagtcatga
    27961 atatcaaacc atcccattat ggaaaaacac cctctttatt caaaacggaa gttacaccgc
    28021 cgctgatatg caaggtcttt cttttacatt aactaattta ggtactaaac aacttgaaat
    28081 tagagagcag gaattttacc gacaaggagt tttggcggta gcggttcgga aacagatcct
    28141 acaacctgga gaaattaccg acgtatttat tatcagccgt ctgggaggtt gagatgaggg
    28201 tgaaagtaaa caaatttaaa ctttcagcag cgcgaaaacg ccagttagcc atcgcgggta
    28261 tggttgcggg cagcatggct gcgattgtgg gcagtgcaat ttatgtcatt gaaggcaata
    28321 aaaagaaatc tgccgaagtc acacaggttc aaaacaaaca acagattctc aaaactgatt
    28381 ttacaagccc ccaagcaggc attacggaca attccttatg gatgaacaca gaaagttcga
    28441 agattgaaga tgcaaatcga aaaatcagcg aattggaaac catggttcag gagttaaaag
    28501 aaaaagagaa cagtagtaat cctgatacaa gtaagggact aggacctgat ggattaggca
    28561 aaccgcctgc aattagcggt attggagata acggccagct tcctccagct cctccagcag
    28621 gcacattgcc gaacggggtg cctccagcag ataggtccat tgaacgaaaa attgtttcag
    28681 gaagtatgag tgaagcagag ctgcaacctg gaacgactgg agcagtaaat accggaaatc
    28741 cgaatgaaaa tgtacgggtt tccccacaat taaaagaagc ggagtgggtt aaatcaaaaa
    28801 ccccgcgtat gaatatagaa gtagttgaag acggcggtaa ggttgtcgtc tcgcaaggca
    28861 agtttcgggc gcgtgactcg tatataccta gcggtacttt tttccgatct gtactgttag
    28921 gtggggttga cgcaccaacc gggggtgagg cacaaaatgc cagcccccac cctgttttaa
    28981 tgcgcgtaac ggattttgcg caacttccca accggtttaa atacaatttt agagaatgtt
    29041 tcgtaaccgg gcaggcttac ggcgatatat cttcagaacg cgcttacatc agattgcaga
    29101 atctcagctg tgtaggtacc gatggccgag ccatcgacat gccggtaaaa ggttacgttg
    29161 ccggtgaaga cggcaaaaca ggtgtgagag gtaatcttgt aacgaaacaa ggccaactct
    29221 tagcaaatgc actgatgtct ggcgtgatta gcggtatggg taagggcgta tcagaagcat
    29281 ttaaggtgac gaataatacc gcatttggca gcacaacctc tattcgaggc agtgatcaat
    29341 atcgtgcagg aattgcatct ggtatcggtg gtgcagctga caggcttgct gagtattaca
    29401 taaaactggc cgataaggtc ttcccggtag ttgaagtcaa tgcaggccgt caagtcgatg
    29461 tcgtactgac tcaaggtatc gaaattgata ccggcgaaac taaataggat ggattcaatg
    29521 aaaaagaaaa tcttggccgt aattgctgca atcacagctt ttcagacggc ctttgctgat
    29581 gtcaaaactg ttgaaagcac gctgcgtaag ttgtatccga ataccacatt ttcttcagta
    29641 aaggctaccc caatggcttc aatttatgaa gtgacaatgg gtgacaacat tgcctatgta
    29701 caagaaaacg gacggtattt catttttggt gcgctatacg atatgcaaga acagaaagac
    29761 ttgaccgaga tggctcgttc agcggtcact caaaaaagtt attcccaact gccatttaaa
    29821 aacgccatca aaatcgttaa aggtaatggt ggtaaaggta agcgtgaatt tgcacttttt
    29881 tctgacccag attgcccgtt ctgtcgtcgt ttggaagaaa cattggccgg catgacagac
    29941 tataccgcat acgttttcat gttcccgatt aaatccctcc accctgatgc aatttcaaaa
    30001 gcagaacata tctggtgcag taaagatcgt gaaaaagcat ggaataacta catgctgatg
    30061 gataaagaac cggcagccgg taactgtaaa aaccctgtat cagaaaatat tgcactcgca
    30121 gagcagctta aagttcgagg tactccaagc atgattcata aagatggtcg tcgtacatcc
    30181 ggtgctatgc cgcgtgccga attggaaaaa tggttaaatg gagcgggagc agaatgatgc
    30241 gtttgaaagc cttatcaatt actgcggcaa tattgattct tagcggatgt tcaaccttaa
    30301 ccatgtccgg tatcggcggt agtgaaaaat ttcgatgctc taatgcctgg aacagtgacg
    30361 atccttattg tgaatctata agcagtaact ataaagcatc tgttgcaggt gttttaaaag
    30421 acggtatgaa aagacaggtt ggacaacctt acactgatca aagtgttcgc acattaatgt
    30481 taacgcaagc ttattcttcc ggcacgcccg ttcgctcaca aagtgaaatt gctcgcattt
    30541 gggttgcacc atatcttgat acggatggcg atttaaatga ccaaagtttc acatatgtcg
    30601 ttctgaatca gggtgattgg ttaatagccc acaatcagca acagatcata gatgagtacc
    30661 gtcctatccg cttattgggg aatggagcca aaacttcaga cagtacggtt gatccttctt
    30721 caaccaattc aaataacaca aatgaccgta tccccgatgt tggcgtgaat cttaatctac
    30781 aggaaacatc gggtattccc ggtaccactc gctagaggta tgtaatgggt gtcctgtcaa
    30841 aatttaaaaa ttggttagct ggttcggatg atgtcgatca accgatggaa gccttgcaca
    30901 acaaccctgt tgatcctgac tttgaccatt tgagcgacta tctcaattat ggtgcatacg
    30961 ataccaaaac agagttgttt actctgatgt ccgttaaagg cgacaaaccg ttgggtatgg
    31021 gctttgtttt tgaactcaac ccatttttag gtggcagtga tgacctgatc agtaagtggg
    31081 gcaacctgta tgcgttgctt cctgaaaaaa cgccgataca agttcagatt ttcggctccc
    31141 ctgatctgag aaactacttc aatgaatatg agcaagtcca agcatcacgt cctatcgacg
    31201 acccgatgcg aagcaccttt gaaactttgg ccaagaaacg tactgaattt tggaataagg
    31261 gtacacaaca ggtcttagtt gaaaatacgg ccattcgttt acgaaatctg cgctgtaccc
    31321 tcagtgtaaa tttgaatatt tcgcctgaca acggtgttga agtcagtaag gccgtttcac
    31381 tccttgcccg tatgcgcgat gcactcatct ccatgaagat ttatcaccac acttgggatg
    31441 caaatgattt attggcttgg acaacactcc tactcaatcc taataggatg tttactggac
    31501 aacaaggtga aatcgatcct gtttgggacg aaactaaatt tttgagtgaa caaatgattg
    31561 agacccgcac atctatcaag gttcttgata gtggctcggg gctgcgtttt ggcaaccgtg
    31621 ctgcggggga ttgcgtgatt gctcaatgtt attctgccaa tcgctaccct gaagagttcc
    31681 atttgtccaa tatgggcgca ttgattggtg atgccattga agcaaacatg aattacacat
    31741 caccatttct gatttccatg gcgatgttca agcgtgatta tgatacttcc agtaataccg
    31801 taaaactaaa ggctgctcgt gccaaacaaa cggcggaatc caagatggcc gcagttatgc
    31861 cggaggctgc aaaaatcaaa cgtgattacg acatctgcct agaagcgttc ggtaaaggcg
    31921 gcggcggtct ggtgagtttg ttacaccaag ttgtcatttg ggagcgtccc gaaaacatta
    31981 acttggcaga aagccaggcg gtatcgattt ggcaggctca gggcttcggt ctttatcgtg
    32041 accaatatct tcaactgggg tcatatttaa cggctctccc aatggctatc gacaaagagg
    32101 ttgagaaata tttggacagt aagaaaagat ggtcaactaa aaccatgact aatgctgtct
    32161 gtatgtctcc cattatcggt gagtggcacg gtttaggaag acctgtcatc ggtttgttcg
    32221 gaaaacgtgg ccaggccatg ggaatcgact tatttgccaa cccggcaggt aactataact
    32281 ttgccattat tggtgcttcc ggttccggca aatcattttt tgccaatgaa attgtgcgca
    32341 attacatggg gctgggaact caggtatgga ttatcgacgt tggccgttcg tatgaaaaat
    32401 tctgccaaat gattggcggg caatatattg aatttaccta tgaagcaggc ctgtgcttcc
    32461 cgccattcca aatggtaacg gacatcaacg aagatattga gatgttgaaa ctaatcttct
    32521 cattgatggc ctctccgtca gattccctaa cggaacagca ggaagccaag cttcaacgaa
    32581 tacttgaaga agtatggtac gaatttggac aagaaggcac ggtcgatcat gtaagggaac
    32641 gctgtctcaa gtgtttgatt accggcagta atgaaggagg acaacctggc gaacgagatt
    32701 tgtctatggt agctgttgcc gaccaattgt acacatactg ttcggaaggt atctacggca
    32761 gttacttcaa tggaaaattg aatgtcgagt ttaaaaatga cctggtcgta ctggaacttg
    32821 aagagttaaa aaccaaaccg gatctgcaac agatcatcat gcagctgatt atgtaccgca
    32881 ttatgcaggg catgtaccta tcacgaaacc agtacaaaat tatgatgatt gacgaggctt
    32941 gggctttgtt gggtgatacg ggtacagccg ctcgttttat tgaggaaggc taccgccgtg
    33001 tccgtaagta caaaggtgca tgcggcactc tgacgcaagg tgcaaatgac tactacaaga
    33061 ctaaggcggc tgaagcggca ttagagaatg ccgactttat ttttcaattg cgtaccgggg
    33121 ctaaatcgct caaaaccatt caagacaaag aaccttttga agtcaatgat gggattatgc
    33181 agattctccg aactttgagc aaaactgact actacagtga aatatttgtc tctaccccga
    33241 taggttatgg agtaggtagg ttgtactcgg atccattcaa cgtgcttgcc tcaagttcaa
    33301 aagcggaaga cgttgaagct gtaaaccatt acagaaatat gggattaaac acagccgaag
    33361 ccattgaagc agttttaagg gaacgagccg caacccgcta attatctaga ggataggaat
    33421 ttaagatgac tgaagcaaaa gaaaagaaag aaaacacggc aaataccgct ccggaaaaga
    33481 atccggagga taacggtgta gaggtttcat tgggcagcct tatgattggc gcaatttcta
    33541 ttgctgcatt ggcattaggc gtatggaatt tcatgaaaac caatgagaga cctgtccata
    33601 ttgtagattt ggcaggtatc tccatgactt accaggaaca ggcgaagaat caaggactgc
    33661 agaatggaat ttccgaccag caacgagccg ctatcctcca aaactaccaa gcaaagatga
    33721 ataccttgca acaggttatc gaagaaaagg taattcagtg cggctgtaac atctttgtca
    33781 aatctgcatt ggtcggacgg aacacagagg tggttgatgt tacggctgac attatgaaag
    33841 agttggataa gcgtgtccca gccacaactg tagtggcggc accggtacat caacaagttg
    33901 aaaagacaac tccgaaaccg atggccgaag agggtaaagg taccccggtt actgaagaag
    33961 atatggttcg ttaatgtttg aagcaatgat aaaaatatca caaaaagaaa gcaacaaagc
    34021 agtgatgaaa cgtctttatc gggagttttg tcagaaatat gcaacccctt ttttagcagt
    34081 tgtggtctgc ttgtcggcat tcaatcaaca ttatgctttg gcatttaatc tttcccattc
    34141 gttacctcac catctttact tcatcaaaaa agatgcgaac aagctaggtg atctgaagca
    34201 gggcgattat gtggcctttg catggcaagg tggtttttac cctattggta cgcaggtcat
    34261 caaagaggtt gccggacttc ccggaaatca tgtaacgaag acgaatcgta cttttttcat
    34321 agatgggaaa gaggtaggta ctgcaaaaga atactcccag gagggcatga agctggccat
    34381 caacccattc gaaggggaaa ttccacaacg gttcatgtgg gttaaaacag ggcacaaaga
    34441 cagcttggac agtcgatatg aattatccgg cttaatccat tcagggcaaa ttattggtaa
    34501 ggcggtcccc attttttagg agtgagtatg tcccacacga aaaaaatagc ttcagtcgca
    34561 tcgctagtaa tacttgcaac tggaatttcc tcatgggtag ccgcttcgac accctctgtt
    34621 gagaagattg gtgacaaaat tggcacaaca tacccaatag cggaaaaaca tgcaatccgc
    34681 gaaattgaag cacgtttgaa agctaaacaa gacagtggcc aattggcggc aatgcagaaa
    34741 gaggtccaag atagaataaa tcgctctgcc ttaaatctac agcccttgga aggactggcg
    34801 aaggcaacta aaggctctgt tcgatacttc gacccaagtt ataccttaac cgaaacaatt
    34861 tatgaccaag acggtcaaat tattgctcct gccggtactg tagttaaacc tttggaagta
    34921 gccccaatcc tattcaaaat gtttttcttt gacggccgag aacctgatca aatcgaactg
    34981 gccaagaaac ttgcagcaga atatggcgaa tcattcatgc ctattctgac tgcaggcaat
    35041 tggtatgagt tatcactaga aatgaatcag gcggtttatt acgaccaaga agggcggatg
    35101 tccaaaagtt ttcagctgaa tgaagttcca agtttggtct cccaagaagg ggaccggctc
    35161 cgtgtagagg tcatgaaacc gtgaaaatat ttgtcacacc gctgaaatta ctgattccgt
    35221 tattactact cccattattt tctatatctc acgccgctga agctgttcct actactccaa
    35281 tatcgaataa attaacctgc cacgggaaga tgcttaatcc gataaatgat atttgttgga
    35341 gctgtatgtt cccaatcaaa atcggcggca aagtcaatct gaatatggat aatcaagagg
    35401 ataacgactc ttctccaagc aattggtttt gttcatgtac gcaccccatc cgttcaggcg
    35461 tgacggtatc attttgggaa cccacacact tggtagaagt cgtacgtact cccttctgta
    35521 tggttagcct aggcggtatt tcacttaaaa atcaaaaagc agataaggcg gaaacaacag
    35581 gtaaagcaga cgtatccaat aagattcaaa atctttttgg cggctcgcat aagcgtggtc
    35641 gtaacgaaca acatgatcag cataatgcct tttatcaagt tcattggtat aaaaatccgg
    35701 tcgtattttg gttggaagcc ttattggaca acgattgtat agaaaaaggt actttggata
    35761 tcggttggat gacagaactt gatcccactt ggaaatatcc atcattggcc gtttatcaag
    35821 cccctgattc tgcgctcttt gccaatgtgg ttgcacaagc agcctgtgcg gctgactgtg
    35881 ttaaagcagc caccggcttt ccattcaata aattgtattg gtgtgcaggt tgccacggat
    35941 cgatttaccc tttatcagga tggatgtccg gtcacttttc cgaagtgcag gccgctaaat
    36001 tgatggtggc tagaattcag acaaaaatgc acagggattt agtcgcttgg agaacatcag
    36061 gcaaggatgc tttatgcggg tacaaactta atcctgtaat ggataaacag aactataaaa
    36121 tggctatgac ctatccggtg gctgcccgaa aatttagagg ccgttgctgt gaacctattg
    36181 gacgttcaac attagtcaga gacagtggca aagcatttcc atacaaaggt aatcagtttg
    36241 ctttccagct tttccgtaag cgcgactgct gcggtgcata taaatttaag actaattgac
    36301 aggagataga gtgatggaca agtctttacg caatgtcatt gtagccggag gtgtcttcct
    36361 cattctttta ggcggtttgg ccggagtagg cagtgcttac gccgatgcgg atgtagaagc
    36421 agcccgtaac cgtctgcgta caaccatggg taaagttacc caagttaaaa acccaaatgt
    36481 gggtgacaat cggcagcaac ctgaaattcc ggatttggaa ggtattaaac atcgtgtgcc
    36541 cgatgttcgt gattacggaa atgcaaaaat caaggctgat ccaatgtcta ttgcccaaaa
    36601 ataccgccct gcttcacaag aagcccgaca ggctgcagaa aaagcggatt taatggtatt
    36661 cgtatcattt tcaatgcccg aagccagctt gaaacgcatt gcccgtgaaa cagcaaaggc
    36721 aggcggggta atggttattc gtgggtttaa agacgactca ctgaaagcca cggttaaggc
    36781 ggccgaagaa cttgcggctt tgcaaggcga tatgctgatt catcccgaac tcttcgacca
    36841 ttacggcatc accgaagtcc ctgttacggt aatcgcccga aacagcgaag atggtttaga
    36901 cggttgcgct gtaaattcag aattgggtat gtgtacagaa catgtcgctg taaagggtga
    36961 tgtgtccttg catgcggcgt tagaacactt gatccgtgag tctaaagaca aaaaactttc
    37021 cgatattgca aatgcgaaat tagccgtttt ggaaggcaga aaatgaaaag acatgccatt
    37081 tatctaactg tatttggcct catgcagacg gcctttgctg cagattacgg tagttacgtc
    37141 ggcgaactgg gaaaattggg gactggaact gcaacaaaca tgatgcagcc tgcccaatat
    37201 accccaagat atacagacaa cgtaccaatg gctgaacagt attacggtgg cggagtttct
    37261 cttccgactc agtttggtga cgccaaattc aacgactgta aaaatctgcc tgccaatccg
    37321 gatctctatc ttcgtcaaga atgcgaaggt gtaaacctga ttgcaggaag ccgtaccaag
    37381 cggcccgatg taaccatatc ttcaaatgaa aagttggtta caggcacgca gaaaattgca
    37441 ggcgatccgg cagaaacact ggaaaaatac aaatggaaat atcccgtcaa tgaagacggc
    37501 agtgtcggca cagttccctc atcggcatgt cctacggaaa ccattactgt tcctgcggta
    37561 aaaaccatca aacgttgcaa tgaataccat ggcgcagaga tgttcttgtg cgaggcggcc
    37621 cttaaggtca atgtcgatcc cagctggaac tattcctgcc tggagagcaa atatcgaaac
    37681 cagcagtatc aatgcggcaa gaaattaaag gtggtatgcg agaaatcagg accaaactgt
    37741 acccgttcag gtatagaagc aggcagcctt cagggtgata tgacggtcaa tatgacgcct
    37801 tcgggaagcg cacacaggct gatgttcggt actattggcg acaactattg gcgtgacggg
    37861 caatatgatc gggaaatgac gctcaatata aaaaatccgg aaaaactgac gctgtttact
    37921 ttgagccggg ttgagtacga tgattggttg attgtcaaag ttaacgacaa aattgtgtac
    37981 agcagctata acaatcagat gtttgcggta gattatactg cagaagatga agagggattt
    38041 cctataggaa gaaggtatgc accggttcgt aatgaggagg gtactcggtt gggttacgct
    38101 gaaataggtg agtcctggag gagaaatatc atttggaccc gcaccgttgt cggaggcggc
    38161 ggagaaaatg ctatcttctt taatgtacaa caatactgcg atcccgtttg tcacgataga
    38221 tgggaaaaca gctgtttgga gtatgagcag agggtaaaac aatgagggct ataacgtctt
    38281 tgctggttat gatctgccac tttatgttaa tcacttcagc gtctgctgcc gcactcaggg
    38341 aaagtgcagc gtgtacccgg acgtcttccg tttgtgttga tggcccgtcg accaaaaaca
    38401 tcaacggggt tgacgtaact aaagactgtt gggaatacaa agaagagtat cagtgccttg
    38461 agaaggattc tgccgattat tgtgcaccgt taaaagaccc ttcggccaaa tgtgaagtac
    38521 agggacaaac ctgtttggag cagtcgaatg agggggagtg tttgcgttac acgcacaagt
    38581 attcttgcga tgtggatttg agaacgttac atcaaggaag acttccgccc aaagtcgaag
    38641 aaatggagca tacccatctg attagttcgc aatgggatga atcatcatgt caggtacaag
    38701 gaaaaaaatg taaagcagtt gcaacagagt gtttggaacc cggcagtacc aagatcatca
    38761 acggtgtacc cgttacacgt gattgctgga aagaaaggcg caccatgcaa tgtacggatg
    38821 gaagcgattc cgaaacatgc agcacttata catcctcaga ccaatgccgt ctgattgagg
    38881 acaagtgtac gcatcagctg ccggacggta cctgtcaagc cagagaaaaa cagtttgaat
    38941 gtaccgaaaa aggagagacg actaaagagg tatccagttg tcaggatcgg gattttgcca
    39001 aaactatgac gatgatggaa ttcgcccgtg agacgcaacg gttttatgat cccgaaaaac
    39061 agcgtttttt caatggagaa gccgggcaat gctctatcaa gctgaatggt gctttagaca
    39121 gcgtattcgg cggagactgc tgtcgaacaa aagcagaacc gggcaaattt gttgacttcg
    39181 cggtacagac agggacaact atggcaacaa cgtattttat ggccagcgtt gccagccatt
    39241 atacgtttac cactatgttt gtcagctccg cagcacaggc aatgggtacg gctctgagtg
    39301 cggccggcgg tattaccgga acatctcaga ttagtgcgtt gggtttctct gctgcgggac
    39361 aacagggtat gggggtaatt gtcggcttca atccggtcgt atttgcggct gctattgccg
    39421 taatcgccat ccaacagtgg ttgaagtgcc cgaaaagtga aattttagtt gccatgaagc
    39481 gtaaagcaga tttgtgccat tatgtcggtt cttattgcgg caacaaaaaa ttgggggtgt
    39541 gtgtaacaat gatagagagc cagtgctgtt tcatttccaa attagccaaa atagtcaatg
    39601 tcggcggcaa agagcaattg aaacgggatt ggggtacacc ggagaacccg aaatgcgaag
    39661 gatttaccgc acaggaatta gaacagttag acttttccaa acttgatctg tccgggtttt
    39721 atgaagaaat ctatgccaac atggacaacg tggccaagca ggagcaaaag gtttcacaaa
    39781 aaatcaggga gacatctgtt aatgggaaaa atttggaggt gaagaactat tatgaatatc
    39841 aataattggt tggctgcgtc ggcgggtatt gtatcgggta tggtttttgc aggtatggca
    39901 cttgccgcat cccttcctta taccggtggt atcgctttgg aaaaaacaga ggctctagca
    39961 agaacttcca tcccgcttcc tgacggcaag ccggatgcct tgcttgcgct ggcaatgcgt
    40021 cacggttcgg caaaagggta tttcagtggt cgcgctgcgg ctgccgtcaa acagaaattc
    40081 cggcaggaga tacctatttt tgtcgaggcg gtcagaatgg aagaagttaa aaacaaaccc
    40141 agatgcaaca gggttcgctt gacattccat acttcgcccg cactgttgca aacctttccg
    40201 tctgaaatta tggatattga ggcgtgcccg aacagaaaga acggtggtaa aaaatgaaaa
    40261 gagtgtggat gaccgtcatg ctgatattta gcctgcacgc ctccaatctg cgtgcagcgg
    40321 atgatggcat gggatggtat gaccaagata tttggaaaaa cgaaaaccgt gctttcttat
    40381 ggtatccgga tgctaaaaag cccaaacaag ccagacaaaa ccagtctcaa aatactccgg
    40441 ttcgtaatga acttgcagaa tttgaaatgc tgcaaagaaa tttggatgag tcgcgtaaaa
    40501 ttgccgtcat gaatcccaca cctcaaaatt tgaagcgata tattgaactt caagaggttg
    40561 tgatgaacaa atctgcggca tttgccgatc aatggcaaag ggtaatttgg caaaacccaa
    40621 atttggatta cagccagcgt ggtcgtccga acaatcaact ggcccagcaa caatatgatc
    40681 ataaccgtca gcaacaaaag gctgaggcaa ttagaaaact tgcaggagag aacggcatta
    40741 tgtttgtttt caaatcagat tgcccttact gtcataccat ggcaccgatt ttgaaaaact
    40801 ttgccgacca atacggtatt aaggttatgc cggtcagtct cgatggcgga ggtattaatc
    40861 acttcccgcg tgctttgccg aacaacggta ttgcccaaag gttgaatatt actactgtac
    40921 ctgcaatgta cgtcatggat acaaaaacca agcaatttac acctattgga tttggggtaa
    40981 tggcgcaaac cacacttgag gatcgtttct tagcgtattc gcgccctgtc gggacattat
    41041 attaatcaaa ttagaggccc ctatataggg ccttcaatat ttatatttta atgtattaac
    41101 tggttgacaa tgcggatatt ttaggtaaaa taaagttccc ttaatctcaa gtctttactg
    41161 taacttatct tggttatagt aaggattccc ctgctataac cgcttcatgg agcggttatg
    41221 aaatttcaca tctttcgatt tccaaagcca gccaaagtgt tggctgtttg tgtttcctct
    41281 gcaaaatatc tatgcttgac ggcatcgctg actcttccaa tcaatagtag tgccggcata
    41341 gagaagaaca tggctgacat gtttaactca atgggagcgg aagccaatta tactgaagca
    41401 ggtgcgttcc atggtcaaag cggtaaccta tacaccggag gcagtatgag tgttcgcttt
    41461 ccagtgtcgg atatcagcct tgggaatatt caattacctt caatcagtgc cggttgtggc
    41521 ggtatagatt tcttcgcagg ctctttctcg tttgtcaata aagaacaatt catccaattt
    41581 acgcgaaacc tgggcaataa cgcagcaggt gtagcctttg acctggcact taaggctctt
    41641 gatccgatga tccaagatgc aattggcggt attcgtgatt tggtcaatca tgttaatcaa
    41701 aacagtttaa acagttgcaa actgagtcag gatattgtcg gcggcgcaat gggaaaactg
    41761 ggttcgttgg ccagttcaaa ttgtcgcgcc gcttcagttg attcgggttc tgcaaatgat
    41821 ccggcagaag gtacttggta ctgtcaggct gccagaaacc ttgttcatga agccgataag
    41881 gtgcgaaaca ctgggaaacc acaagatacg attagttata ccggcggtaa tttgacttat
    41941 gaggccatga agaactatac aggtcaacgc gctaaactgg gtatcgacat ggacttctat
    42001 tacagcatgc tgggaacagt tgtgttcact ccattaacac aacatgaagg ggataaggac
    42061 aaatacacag taggtgttca aagtttcgaa ccaaaaatcc tcagcatcca agatctttta
    42121 aacggaaaaa acaatgccgg gggagaagtc cgggagaaga ttgtattgga tatgtggacg
    42181 tgcaaagacg gttctaagaa attaaaggag aactgcactg agaaaaataa tgtagaaata
    42241 ccttctttac ggtacatggt tcacaaacgt attgaaacgc ttccgactgc aataaggaat
    42301 aaccaaggtt ggaatttatc tacgcgcaag cagattgcag ccattgtcaa taacagtaag
    42361 ttgcccatat taaaaatggc tgtatccgac gcatttcttg gcacaggcta tctgaaaaaa
    42421 acagcggttc tagatgctat tgccgtagat attatggcca atatcctaga tcaatctgaa
    42481 cgccaggtac gctcgacctt gggtatgtat aacaagacag acgatgccag cggggaagcg
    42541 gttcgcaaat tatttgccaa ccttggtgaa ttacgaagca gaatttacca ggagcgcatt
    42601 gactcaatga agaaaattga gatcgaagaa agcatgatga gggctatgga gcaatttgaa
    42661 acacaatggc gctcagcctt tgtcgatacc aacaactcca tgacttttga ttcaataaac
    42721 cggttttagg gggtgatatg gctgttgaat atttcacgtt tcccggtaat ggcgaagtag
    42781 tacaggcagt ctttaacggc gtagctatga tttccggctc caccatgatg ggaggggcaa
    42841 tgcaggctgc cgcactgttc ggttttctgg ttgccttgtc cgtagcagtc ttcaagctcg
    42901 atctgcgcga aagctttact tatttgtttg tggtcggctt tttttggatg ggtatgatag
    42961 tccccaaaac cacggttctt attaccgaat caggggggta tggatatacg ggtcgtcaat
    43021 acactgtcgg taatgtaccc gtaggccttg cttatatggg ctactttgtc agttcttttg
    43081 gccaaagtat tacacgcaaa gccgagcagg ttaaccacct gccggatgac ctcaattatt
    43141 ctcgtaccgg tatgctgttc ggaacccgcc tattggaaaa tattagggaa gcacgaatcc
    43201 ctgacgcgca actgactcaa gattgggcac tgttcatgaa tcagtgttca ttctttgata
    43261 tgaacctgta tcacttctat aacgtgcagg atctggcaca gagtgccgat attctggcta
    43321 cattgaccca taccaaccaa gccttgttta ccaatgtatc gcagatcgcc tcaagaagag
    43381 gcaacatgtt gcagtataac ggcaaatcaa agaccatgac ctgcaatgag gcggcaaaag
    43441 aactaaaaga tcgtacccag ttttatagca aaaattactt gcccggctac attgccgatc
    43501 gtgtttttgc aggcttgggg acaaatgctg caggaattaa ccgggtaaat gccctggtta
    43561 ctttaggcaa tagctcattc caatatctgc tggataatgc ccgcttcgat acattgaaaa
    43621 atatcgagca agctgcgatg gttgaagtta ttcgtcaagc aggcattatc aatggccaac
    43681 gcaaccgaaa ttcggctgcc gtacagcagg catttgctca agttcaagct cgtaatcagt
    43741 atattgcggc gcaaaaaacc ggttcatcaa tggcaagttg gaatcttcct ttgattcgtt
    43801 ctgctgctga ggccatcatg attggtttat tcccctttgt tatgattcta gccttgttgg
    43861 gcggcatcat ggcatttagg atgctcggtt tctacatgat gggcatgatg tggatacagt
    43921 tatgggcacc cgtagccagt atcatcaatc taatcatgac catgaatacc aaacggctac
    43981 tcagtctgga aacccttaac ggtgcgatta caccgggttc cagcgatacg ttgctgatgg
    44041 cagctgccga tgcacaagca gcggcagggt ccgccatgtg gctgattccg gtcatcgccg
    44101 gtgcactaac tatgggcggg cgcagcctga tgaacggcat gatgggcatg acttcatcgg
    44161 ccaaatcaac tggtgaagca gcgggttctc aagtagggtc ggggaattac tctgccggca
    44221 atatgaacta caacaatagc aatgccaaca aacatagctt aaaccctgta tataccgatc
    44281 ctcaaatgat gactgctcaa tctgcagccg gaacatcatg gcggaatatg gcaactggag
    44341 aaaatcgcta tcagggaagg ggggacagct ttggcaatgt ctcatctacc tctactgtca
    44401 gtatgtctga tgtttacact caagcagccg accgaagcac gacggcagcc aaacagtatg
    44461 ctgcgtcttc agttgaacaa actacattgg gtaatacaca aactttggct ttcggcttac
    44521 aaactgtcag tcaaaacggt gcgaattatg cagttaccaa tggcatgagt agttctaccg
    44581 ctaaagcttt ggatcaaatt tttcaatctt caaaagatat tgctcaaaga aacggtctgg
    44641 gcaacgatgt acagacagcc agctctattg cgcttgattt gggagccaaa ttggcaggcg
    44701 gagcaacaac gccatatgct agtgcgacag taagtggagg agttaaagcc gaaggtcgtg
    44761 gtggttcaac caacaaacta agccatgata ttagcgaaac agccaaagcc cttaaatctc
    44821 agggtgtcag cattacaaga agcatgcttg aaaatattgg tcaaagtaat gcgtttactc
    44881 aagctctaac tgacagtagc agccttgctt ctcaagctca agccagctat aacaaagcac
    44941 atcaggccag tatcagcgaa caacaaagct ataacgaggc tcaaaactat cgcgaagctg
    45001 cttcccgtac ggcacaatcc ggtgcgactg ttagtattga taacgcaaaa gccattcgag
    45061 aatatgctga tcagaacggt ctagcctaca gccaagtgta caataatccg tctattgtag
    45121 cagctgcggc aatgaatgca gcagttactc aagcttcaaa tttaagacag caagctgaag
    45181 gtacccgttt tgacagtaca ggaccgatta gtcccgatga tcgccccact aataatgtgg
    45241 ctgctgctta tagtagttat aagtctggga tagacggcca aaaatctagc acttttgcca
    45301 ctatccaggc tcaagctgac cgaaatggcg ttaatagaaa tgaagcccga gcaaatattg
    45361 acggttggca acaaaaacta gaacagggaa gcaaagatgc gaagaacttt aatcagaagc
    45421 tttttgataa aggtatgaaa aaacaagaag aaaatacaaa caaactgcat gctcaaggag
    45481 aagacacctt caaacatgta aacacttttg ctatgggtgc tacacaaact ttctcttccg
    45541 ataaagcata tgtccaagcg caaaataaat atggcaagac tgaagggcga ccaggaagtg
    45601 gttctcgtaa ctatcgccca aagaagtagc cccgcccggt atcggctatc gcggcaatga
    45661 ggcaaagtca tatggctcag gaaatgaaac tttgggtcca ctattaccgg gcgcacatac
    45721 tatcatcagc ccatttggaa aacgggttca ccctattttg aagaaagtca taaatcataa
    45781 aggcgaagac tatcgtggct ctatcaatac gccattgctg gcaccgttta gcggtagagt
    45841 agtggaaagt cgctatcaag cgaatacagt caacggtaaa accgtcggct ggggacacac
    45901 tgtcgtaatt gaagcgccaa atggcgtaag gtatcgggct tctcacatga atggtgccag
    45961 cccgctgcgt tttggtgaaa cagtaagtaa agggcagcaa atcggaaatc ttggagccag
    46021 tggtggagta acaggtccgc atcttgatgc catgctgtac tctccatcgg gtaaaatttt
    46081 acctgtgagt gcctatcgcg gaaaaacttt aaatgaaatc ggtaaaatca aatagagaag
    46141 ggtacatatc atgagcattg cccatattgt cattgaaatt gaagaatctt cgactttgga
    46201 tgactgcaat gtacggttaa gtttcagtgg cggtacagaa ttgcagtcag acgaggcaaa
    46261 gactgtgttt tataaaaccc ttgaagcaat tgagggggtt ctaggagacg attaaaaaaa
    46321 acaggcagtt cttttaactg cctgtttttt tattaatcgt catttgatcg atgaaatata
    46381 ttgcaactga gattggaata agctgggtca taaatgaatg gatttaatga atcctcagat
    46441 aaccaatcat tattggtgac aacatcagca tcagacccca gtttcatatt cctgaatatc
    46501 ttattcaaaa agaatgtcag cacggaaaaa gttaccgcag ggaagacaaa cagcaaaact
    46561 atgttagtct ccctatcagt atcaaaaaag ccggctaaga tgtagaaaaa gcccagtgtt
    46621 atgaacatag tcaaacacag cgataatata gctgccgaaa gcaagttgtc ttttttagac
    46681 atgggttgct tttcaacttt ctgaaagtct ttgcataagg tttttaattt ttggaactgt
    46741 tgaggatcag gattatgtat atcactaacc tttcgcgctc gcagcccacg actatgctca
    46801 aatggttcga aattcactat ctgtcctggt atatctgaac cggtggattt acggaaaaag
    46861 tattcatgac catcttcacc aaagataaag ccaaaatctt tctcatagct ataccatttt
    46921 acgaatccga tcatgatact tctcctttat ttataggtta cttgaatact atcacttgta
    46981 atccaacgtt cattgcctct gtttggctga attttaatta agccattctc acctttttct
    47041 aagaccaagg caggcccata gaaagtggag atttttttac cgggttgtac atccgtaact
    47101 ttgctgcggt aggtttgttc tccggtatat ccctcagaga atgcaaaatt cgtatagttt
    47161 tctctgtacc accatcccat ccactgtaca tctctgccgt tgatacagaa aatatcggaa
    47221 gtcagggttt caactgtccc ctgatgatgt tcgcaatatg ctttgaaagt tcctactgca
    47281 tcctcaaccg ttttttcatg ttcagattcg gatcttgcat agaattttcg ttcaatagtt
    47341 cccgtacttt caaaaatctt gccttgaatg atacggccaa gtcccgccaa tttactgtct
    47401 ttcatccaat cttgtacaaa cttgggatag ggtttatcta aaaaagcttc taataccccg
    47461 tcaattttgg ccggctttga tgcaagattt aagaaattac cttcagcttg ggcatttgaa
    47521 atggccatag ctgctaataa tccgaacatt atttttttca ttgcaatatt ctcctcatct
    47581 tattgaaact tagggtttgc tctaaacagt ggtttatgat ttaaaccaac tggccatgcg
    47641 ctgcagaatc ccatgctttt tcggaatttc ccttttctcg gagtgagctt ggaaattctg
    47701 atgttctaat aaggctgcgt attgagcgac caaagccgaa tattcagctt ccaataaaac
    47761 atgtttattc ctcagtgcct caaatttttc ctgtatttgc tctacttgtt gttcatcggc
    47821 ctttatttga ttatgtaaca tcaaggccgt ctgaaaatcc gcactcaaag gattacggag
    47881 tagggtgcag agaatttgtt tccgaaaggt gttccgaagt ttctctacat ggcgatttat
    47941 gcggattttg atttcttcat tatcataatt gagcagctcg tcttcatcaa tatacaaaaa
    48001 tgaactctta tctgcccgta tctgaaccac cgggatacct attttcgata aggctaaaca
    48061 tttaggcgca ttggaagcca atcgtgatgt atgccaaatc tcaaccgcca attttctctg
    48121 ccaccttatc tcatgcctga gtagggaaga gttacccggc tgatcaaatt caagcaaaac
    48181 atcaatataa aaagttttgc catctatctc tatccgatat tcggataatg cacggttcag
    48241 cctaatagtg acaggcggtt gtttctcggt gtgttcagtg taatttagaa cagtcgtcag
    48301 tccgccgggt aagttggata gttcgttcaa cacttgtttg caaagcaagt gggttagcga
    48361 ttcaggggca gaagcaccac cggattcagc ttcactgtaa tagcgaaaaa aattggtcgc
    48421 cttcctcctg gtcaaaacca ttttctttct tgtaccggga cgatgacggc tgtagtagat
    48481 cattccttct cggtgtttta gctttgcttc cagtgcagtt acccattcat caccatctgg
    48541 actcgtataa gcaataattt tcaaacgatt aaaatccttt gggaagaaaa gcatcatcag
    48601 aataagacaa caataaaatt ctatatatcc tgtttgccgc taaagggcaa ttttttcttc
    48661 ccccttttag atttgtccgg ctcatataca gacatgagca acattataga taacagcaat
    48721 tattattaca atgttgtaac attcagccta atactgtatt ttgaccatag gttatttaca
    48781 gtacccatta tactactgta aaatataaat acaataattt aaaatccagt ggattaatta
    48841 caggatttaa aatacatgag tagcctcaaa atagcacgga tttatcttag agtaagtaca
    48901 ctacaacaaa atttggagcg ccaacgcaaa attatagatg aggctaagga aaatggattt
    48961 tacatagctg gaatctatga ggaaaaggcg agcggggtga atgctagtcg tcccgagcta
    49021 aatcgactca ttgacgatct tcaggagggt gattgggtta ttgccgaacg aattgatcga
    49081 atcactcgcc tcccacctcg tgaagcggaa ttactgattc aaagaatcac cgaaaaagga
    49141 gccaagctgc atgtgcccga cgttttttct ttcggtaact tagatgaact tatgggggag
    49201 ggtgattctc ttccaaaata tttaaccgaa ccacttatgg aagccttgca aaaaatgttc
    49261 ttgcgtatgg ctttaagtat tgcccatgag gattggaaga ccagaagaga gcgcgcaaac
    49321 caaggtattg cacgagccaa aagagaaggt cggatgaaag gccggcctgc aaatgaggat
    49381 ttgcacaaaa gtattatcca actcacatta aacggtgtga gtattacagg gatagcatcc
    49441 gcacttaaat gtagtccttc gacggtaagt aaggttagac gagcttataa ccgcaaacat
    49501 aagtcggggg ataaggatca attcgatata tttaaaccga gtttgaagaa gtaagtaatg
    49561 gaaaaagccc atttggttag tttaaaacaa acattatagt tttggagaag ataaatgata
    49621 aatgaacctg gatttatgct actaatttct gctacaggta tggttttgat atttggtcta
    49681 tttgtttttt tctcccataa agttggtctt gacagaaccg aagcctatca gctaatcctc
    49741 agaagtttta ttcctttttt tgtaattttt gtaattttcg gtttttctat tattctagaa
    49801 tttttttcta ttattctagg gttataaatt gtctctactt cttggttttt agttttatag
    49861 attttgaaac cttcaaactc tgacttatcc caacgactgc atcctgcggt cgttttttgt
    49921 tttcttcata ttgacttgcc atttaaagtt gataaaatga ttttgttaca aaatcaaatt
    49981 aggcaaaata aatggatgat cattcgaaga agcgaggagg acgtagagaa ggagccggtc
    50041 gaaaaagtaa atccggcgaa gcaacggcag taaagcgtat tccaatatct ctgataccta
    50101 ccgttgatgc aatgattgct tctacagcca tgcctcaagg cagccttata cccatcaatc
    50161 ggacaacact aaaagtccct tatactctcg agaaaatccc cgccggattc ccatctccgg
    50221 cggaaccgta tattgccgat tatcttgatt tcaacgaata tctcattgcc aacccatctg
    50281 ctacctttgc agctcgttcc ggcggctact ccatgctaga cgctgggatt agcaaagacg
    50341 atattatgat tattgaccga tccaaaacgc cgaaacatgg cgatattgtg atggcggatg
    50401 ttggtaacga gttcacaatt aaaaggctat ataaaatacc aggacgcaaa cctgaacttc
    50461 attcagaaaa ctcttccggc gagtaccctg actttatacc taatgacagt gatacctgga
    50521 cagtcgttgg tgttgttacc tttgttatca aagatgttcg tcagggaagc taagatgttt
    50581 gcccttattg atgggaacag cttttactgt tcttgtgaaa gagttttcag accggaattg
    50641 aagaatatcc cagtcgttgt cctgtcaaac aacgacggct gtgtggttgc tcgttctgct
    50701 gaagcaaagg cactcggcat caagatgggc gaaccgtatt tcaaagtaag acatttagtg
    50761 ggacaaggcc gtctgaaagc ttattcgagc aactacgaac tctatgcaga cctgtcccgg
    50821 aggatgatgg aaactatcgc cgggtgtgtg ccggcagttg aagtctattc tattgatgaa
    50881 tgtttcgcag atatgaccgg cattgaaaat cttgctgtac atggacagca aattcgcagc
    50941 cgtgttttac aatgggttgg cataccaact tgcgttggca tagcaccggc taagacgctg
    51001 gccaagtttt gtaaccatct cgccaaacgt tatcctgatt actttggtgg agtagtggtt
    51061 tgggataatt ggaatgaaac tacccgtctt cgtgccttgc gcagccaagg agtagatgaa
    51121 attttgggga taggtagacg gactgccgat aaactatctg aacaagggat acgaacagta
    51181 ttagacttcg tagaagcgga tacagccacc atacgccgac ggtatggtgt aacagtggag
    51241 cggacgcaaa gagagatgca gggtattgtc tgtgacgggt tacatctcaa tgcacaaccg
    51301 agacaaaacc ttgttcgtag tcgaagtttt ggaaagcgta tcggaacatt agaggcattg
    51361 caggcggctg ttacgcatca tgtcagttca ggggctgaag cattgcgtaa ggatggttta
    51421 actgcttccg ccgtttctgt attcttacat acagattttt ttcgtgagga ttcgccacaa
    51481 taccacaaca gcctagccca gaatttgatt gtatcaaccg ctgatacagt tcatttgaac
    51541 cgaatagcac aacgactgct ggcagatatt tatcgcagtg gatacctcta taaaaaatgc
    51601 ggaattgaat tatatgattt gaaaccagct attcaaatac aggcagactt atggaaaccg
    51661 acaaaagcca atcctgccat ggaggcatgg gataaaatca accgtcagca cggtagaggc
    51721 agtatgaaat tggtctcaga attactggcc gatgactgga aaatgaacag agatagatta
    51781 agccagtgct ataccacacg gttcacggat ttgctcgtag tctaaggata taatttcccc
    51841 aaaacatcca caaaatatgt ggataacatt ttgaacagat taaagaccgc tccgtgacgg
    51901 tctttgtgaa aataaggtga ctgtagttta gtcagctaag tcctcagatt cgacaagtat
    51961 taaataaaat ggaaaattgc gacaaatata ttctcaaaaa attcttcatt gcttaccgaa
    52021 ttgaatttaa tgcggagaaa caatatcagc gggaaaagga gcggtaagca agggatagta
    52081 taaaaagagc aacagcccat gagaatcttc taagggctgt tttcttttgc tctattcctt
    52141 tgatactact tcaagcagtg cagtccaatc tgaaggagga tttccgttcc ccagcatttt
    52201 tttgaacaca gtatatgcat catttttgcc gccataagtt cttttcgtac ccgagtcgtt
    52261 tacccatgcg agaatgatga ttttactgtt tgagtcgtaa cggaaaaaca atcggtatct
    52321 acccataaac ttggcacgtc gccaaaattt gtattccgag ccgagtgtct tgccaagtcg
    52381 atattccggg ctatccggat taagggcaat attttctatt tcgtctaaga ttcgttccag
    52441 cagtttggtc tcttcggatc tactggattt ttcggatcgt ttctggcttt ctatcgtagt
    52501 ggccagtctg tctaattgct gcgtaaagca ttcgtgttcg taaatggtat atccgtttat
    52561 ctgcataggg gaatcaggca tattagtctt tcatatctgt ttcccaagtc agttggcgaa
    52621 agcggttaaa gcgtgattga gatgtgggtt tgatggaatt cggatttttt gtcatagaat
    52681 tttccaaaaa atgcaggaat tttccaacaa ctggatcatg ctcaaattct tcttcctcag
    52741 tattgttttt ttctagcaat acctgtccgt cgcctaagac ggaaaaacgg attttgtcct
    52801 gtttttccag ccccaatgcc ttgcgtactg actcgggaat ggttgtttgg tttttgctgg
    52861 tcatagttga cactgcatca aaaataatct tcatggtctc tttgccctgc cttcattcaa
    52921 atttactgtt gctacttgcg acttcaatgt cctagttacg cacatcttca acttaacggc
    52981 ttgggaaatt attgatattg ttaaaaactg cttaaattaa ggaattctgt ttattgtaag
    53041 gaaatttcct tacagagtca atacccaacc ccttcgacct gtaagggcgt ttatgaatac
    53101 cccatttact gactggcagc gatgacacgc ttaagtgagg gagcaggcgt agaccggagg
    53161 taattcctgt tcccgagctg tcggcggcga gaccggggga ttgggggcag agaccccaaa
    53221 aatatcagag tatgggcaca aaaaaacggc cgcattagcg gccgttggtt ttgaaaaaga
    53281 ttatttgagg cacaaatgct gcttcaaaca ggcatcgagc ttggattcag gatcacactc
    53341 tccgaaaaac tcatccagga acgctgataa gtccagttca tacaacgtcc ccgtccagtc
    53401 gttataaaac tccaaataat aagagaacga tttgataggg aaaggcttta catccaatct
    53461 ttcgtatctc aactttcctt cgaaaccgaa cgaatccgca ctgcattttg cgctgatgct
    53521 gtgcaaaatg ccctggtttt cgttcaaaag tctaacagca gtgtaaaggg attcaacatc
    53581 gatgctgact tctaagtcgg gtacggtgaa gtcatactcg tccgtctgcc aaatggtatt
    53641 taattcgtca ctaccatcgg gcaggttaag gtaaaggata cgtttgccgt tttcctgttt
    53701 taggatgaat cgtgcttcac atgaagcctg agtgacctct ttgtcaatct gattgtcaat
    53761 catttctaat ctccataaat agcgggagac aaccctgcgg gttgtccccc agggtgactt
    53821 gccaatacgt ctaacgaaaa ctaagttttt taatcatcgt catcgtattc gcatccaaag
    53881 tgctcgatag cgaaatcacg gacttcatct tcttcgcaag gataatcgaa caacacgcct
    53941 tcatgacctt cataggaacc cgcaaaccac attccttcct caaggtattt agcttcaaat
    54001 tccacgtcgg gaaaacgttt ggcgatttct ttgtaaagcc tatccggagg accccaggca
    54061 gtgtcacagc tgatccagat gtcactttcc tcaaccgtca caaaagtgca gtcgggatct
    54121 aagtcccatt tgcagcccca gttagccaac cgccagtcat accaatccgg atgaccgtat
    54181 tggcggatgt tttccgcaat acgcttgaca aatggttcat cgtaacgaaa acgagttgtg
    54241 acttcagggc tctctgcaaa atgggccaat acttcaccaa cagtcagatt gtcccaaaca
    54301 aatttttccg aaaaatattg ctttaattcg gagaaaagtt catccggcaa tctatattga
    54361 acgggccaaa agggttcatc gcgctgttca agcatttcct gttccaaatt ggtcaacttc
    54421 ttttcaggtt gttgagcaat ttgaagccag gtctgctgca tgtgactatc agacccttta
    54481 gtaagattca gctcctctgg catgggtaca atatgctgaa agctgaattg ataccccttg
    54541 tcggttttga ccagaatttg ctccgccact tcggcgatac gttcaggatt gccttgaaaa
    54601 tgaaggctgt tgctgcacca gttgggcata gtagtctcca gagcaggaat ccccatccct
    54661 gcggataagg aaacccgcag gttgatgggc ttgcgcccag gttgataaaa aagtcgccac
    54721 cggaaaccgg cgcacatttc agacgacagg tgctttagat aaaaggcaaa gaaaccgaaa
    54781 cggtaacttt gcctttggga agcggaacga cggttccgcc ggcttgatga aaaatgtgct
    54841 taagcacggt tattttttgg gggttttaag atagattctc gcaacaataa caagaaccag
    54901 gccgactgcg gccatgaccc aagttccggt aacgaagtcc attatttgtc ctccagttta
    54961 gagatgtgaa tgcggataag gaatgaaaca tacagcattt caaacgacag gagcaccaat
    55021 cctgccatgg caagattctt catctcctca ctatacccgt tactccagac caccacaccg
    55081 ataccgataa tcgccgcttt ggcaatatcg tccagtatgg cggcccatga agccagtacc
    55141 gcagtgggta cttttgtttt cgatttcata ttgtcaggca tatacacctc cataaaaaaa
    55201 ggagatgtac gcctgacggg gaatacatcc ccttcagggt tgataaaaga gccgtgcatc
    55261 cgaaacggat gtgcatttgg ttaacggcgg tatgcgggca gtttgacgga ctgccgaccg
    55321 tgtaacatag tctaatcttc taccaaatac ccgtcgcttt caagtctatc ggacaagcct
    55381 tcgtaccaaa tatcgaattc ggcgataccg aatttacgga tgtcggccat ctcgcaaaga
    55441 caatggtaaa gcacgctgtt acggtattcg tagcgtccgc cgaaacttcc gtactggttg
    55501 atgtatttcg ctgtaaaaca tacatccaag ttggcgaaaa tttccgcgag tttgcgataa
    55561 ataccgaaag gcggtgccaa tccagttaaa aagcagattt caataaaacc ctctctttgt
    55621 gtaacaaagg tttcggtggg acatggggaa aaactgctgc cccatgcctc gattctctgt
    55681 ttggtaaggt cagacgtgat attttcgggt aaaggtacaa ttcgatcgaa atcaaaagcc
    55741 cgatgctcgg gataagcgtg ggagcgaaat aatatcaatt tcgccgcttc ggcaaccgat
    55801 tctttggaac cttcaatcag aaagatgttg tcaactttgg tggacataat cgtctccggg
    55861 cgggtattgc ccctgcgggc agaaacccgc aggttaaggg cttacgcccg gttggaaaag
    55921 aagcacggca gttatgtcgg ctctgcacac agcacgggca ggatttcgcg gtgcagccag
    55981 ccggtgacga cttctcgcag cacattgcgg cagccatcag tcagaacatc tgatagactg
    56041 aaaccgaact cgtacagtcc cagcccttca ttcgactgcc actcgatgat ggcattgctg
    56101 tggcggcgga aaatccaggc aatgtcttca tctgataaat actgcaatgc cccctgaaaa
    56161 ataaccattt tgtcaggacg gttgaaatac tgccgatagg cagacatcag atagcgcatg
    56221 tcggcaacgg cggatacgcc ttcaccaacg tatccgtcct caaggtaagt gacggctgca
    56281 acggcatcac cattgctact gctgatgcaa accatgtcgg cattagcctg tttggcaaac
    56341 tcttcacagg tttgggcaaa gacggccaaa tcagccccta atccaaatct tttcagttca
    56401 cgtctaatag cgaccatgac accatccgtg tgagcggtca aaactgcacc gttcaattta
    56461 gctgccgcag ctgccgattc ttcccgatcg ggattcatcc atacaataaa gttttgcatt
    56521 gtgttgctcc ttaataaaga aaacggagaa cacagtctgt agggattgtg ttccccacgg
    56581 ggctgataaa agaaaatgcc gtacaccccg aaggatgcgt gtcatgttta acggcaggac
    56641 acaagacaga taaggtctgt cagcctgtaa agacaggtat ttttagacgg ctttcaagta
    56701 aagtcttcta aaaatacctg ccaaaaggca ggtagaagtt acgatttcaa ccggaaacca
    56761 ccgtattcgg tgcacttccg gaaatcagaa tgtcaatcag tgtctgtgcg gctgcgatgg
    56821 gcatctcgtt gctgatcaca atactctgca acggaaaata cctgcttgag ccgaatacag
    56881 acaggccaaa ctgatcggat ttgaggaaaa aggaaaagcc gggatctgct tcatgataca
    56941 aatgggcctg aaggatgttt tcagaaacat ccaaaaccga ttcgccgttt tcgccaaaga
    57001 ccaaaccgta tttcagggca atatagtttg ccacttcaat caacagacgt tcggcatcag
    57061 acggcttggg atcagacgac agtctgcctg cgtaccattg cctgcgcttt tctgcaggtg
    57121 tgttgaagac tttttcagcc caaaagcgta ccgcttcgct gacctggata cagcctactc
    57181 cttcaagaac ggcgacggca tatagccaat cgtcgaaata atcggctgcc tggccaaacg
    57241 tcagactgct gccgataaag tcggcaaagc cgttttcgac acgataggca aggtcttcgg
    57301 catattcttc aaatgcctta taactttcct ctgtaattac tgtatcgcgg ggaacatccc
    57361 ccatatcccc aagacagtaa ctcatatcgt ccgatttgag gatggcgttg ctgcggtagt
    57421 aattgggata gtgtttttcg ataaagtccc agtccatagc ggacgggcga agcatgaaat
    57481 attttgaaac agacacgata tttcttttag aaaaagggga tatgcgcctg tcggggaaca
    57541 tatccccgtg gggttgaaaa ttaggtttat gctgatacgg cttcgtcagc ttccagttgt
    57601 gaactgcttg ggatattacg gccatatttg tcgaaatact ttcccaatag ggctttggcc
    57661 cacaaaacgg ttacaacttc agcaagattc tcctgtgatt cgagtaggtc aaagaggtca
    57721 gtcctgcttt cagccagcca atggttgact gcatcgcgat ttcgcccata aacccactgc
    57781 aggtctttac cggtcaggtc ttgaacataa tcttcgaaag atgcttccac atcgtccttg
    57841 tttctcaaca gatgaagcag atcggcgcag ccttgatcct caacggcatg ctctaccgat
    57901 acagccgccg cgaacaagtt aaggtctttg gacggccaat tttccgaatg gaagttttgc
    57961 gtgaaatact cgaccgcttc aaagacatct tgggcaatac cgtgtggaaa gcctttatcg
    58021 gacagataat ccgatacctg atcccaaatt gcctgataat tttgtggaca ggtaaaaaaa
    58081 cgtccgttca aagcactcgc agctgcttca gacagttcgt tttccatatc catggtaatg
    58141 atgtaggttt gcataagaaa tactcccaaa aaaaaacggg actgccgtct agaagggcgg
    58201 tcagcccctc ggggttgaaa aaaggaacgg atattccgtt ctgcatattt aggttacatg
    58261 gtttaagaat tttgcagagg atggctgttc aaataccgtc taacggtatc gatagcccag
    58321 cttttgatgg ctttatggat gactttgcct tttgattccg gtcgggaggt gttcaaatcg
    58381 gtgaaattcc cccaatttaa gccgttctcc tccatccatt gaagcacggt ctcttcgttg
    58441 cgttcgaaca gccaggcagc cgttttttcc gaaacaactt gttccaagaa ttcaatatga
    58501 tcggacggcc cgtattccac atctttaagc aggtaggcga tgtcatcgta accgccaatc
    58561 tgcggaacgg ggaccagcac aaagctgtcg ttatcaggca tgccgctgtc gaacaatgcc
    58621 tccatcaaca tgctgtaacc gacatcacgg cctcgcataa tgctttggaa aacctcctga
    58681 gcctggtcat ctgtcaacat gtcggcgaaa ccgggttgac gcaacaatgc cgtccacgcg
    58741 tcatggtcgt tcatgacgga gaaggatacg gcggcggtat ggcggttttc tgtcatattg
    58801 ggtacagatc ccatttcgtt ttccattctg acgtattcgg ccaccggtac gatggcactg
    58861 atcagattgg cggaatagcc catctcggcc aatttgcaac cgatcttatc ggtgaggctt
    58921 tcgtcgttgg acggataaca gatgacctgt ccgttaaggg cctcagcagc cttgccagcc
    58981 acttcaaact cctttttgaa ccaaacgata taagtttcaa tatttttttt cacggtattg
    59041 ctcctagaaa aatgggatac cgttccccat gggtacggtc cccacggggt taataaaaga
    59101 aaatgccgta catcccgaag gatgcgtgtc atgtttaacg gcaggacaca agacagataa
    59161 ggtctgtcag cctgtaaaga caggtatttt cagacggcct tcctaaagtc gtctgaaaat
    59221 acctgcccga aggcaggtga ggtatataac gattacaagg tcagtaatct tctgggaaca
    59281 ggagtgtggt aagacttctg tcccattctg taatgaccca aattttttgg tccccgatct
    59341 tgtaagaaga cattattctg ccttcttcac ctggcttgag tgccagctga ttcaactttg
    59401 catcttcagg gggaagatca ccccatatgc cacggcggtg ggcctcaagc aatatttggg
    59461 cataaagatc attgtcatac agttgtgcag gcaggtgctc ttttgcccag ttatctactg
    59521 ttgccgtcat agacactcgg cccaggcgaa acagataagg ggtatcacat gcagccatag
    59581 ccgccaaatc gagttgttcc attaagtgta tccttaaaaa aagccggata cacccctgcg
    59641 ggaagtaccc ggcagggttt aaaaattgaa taggccaatc tatttatttg attgtcagga
    59701 ctcggccaaa gtccggcgat ccctcggtca aatcaattgt ccgtatgctt ggagaaaacc
    59761 ggtcagttga ggtaatcgtg accacaagtc tatccttgtt gtcgtactct tccgatttgg
    59821 tttctacttt ggttttatgg gttgaacctt taaccaacag ctttcgacca ttggttgagg
    59881 tgacaacgcc attaacttgg ccggcggcta atgctaaagc agcatgccac tgacccagag
    59941 cacataaagg ccggcgtttt tcggctataa cggcattggt gccgaaatgt tgagaaaatg
    60001 atggccataa cgtgcgggat tgctgctgtc ccagttcacg ttccaatata tcctgctcca
    60061 atttaaagct tttggggcga aagaccttat tgggtgcaat aggcacacag tatgcaggca
    60121 taccggtcgg caaagtctca ggggcatttt caaaagcaag caggagcctt tgttgggcat
    60181 ctgcctgttt tttgctgata gaggctttgt tcttcggtcg gatgccgaat ataaccaact
    60241 gtttgtaggt atccaccgca gcccttatca tacggacatc cgtacagtgg gtagcgattt
    60301 cgccggttag atgctctgtc agggcggctt ccggaacaat cattaccaac actccgccgg
    60361 gctgcaaagt accgaaggta cgcgcgaaga aaagttcttc aagccgtttg gttctttgat
    60421 tagacagatt gtctttggcg gcaaagccgt aaggaggatt cagaaacagg aggccgactg
    60481 ctgaaccctg catggtgcag ttttctacat cggatttgat gacactggtc agtcgactca
    60541 ttgcctcttg agcacggttt ttctccagtt caatcccgta agtatgacaa cttatttttt
    60601 ccgattgcaa agtagaagct aaagaagcta gggcttctcc tgttccacag caaggatcaa
    60661 atatgcgtaa caaacctccg gcagatgccg gaatatcaag gcaagcggct atacgcgcca
    60721 atgtggcctg gtcggtgggg aagtatccgt tgtcacggtg gttatgcgca acacgggggt
    60781 gcatcagatg gttttgagtt tccatattgg tctccattca aataaaaaaa acggggacat
    60841 gcccgtgcgg gacagtcccc gcagggttaa atcaaagttc aatctaaggt actgccacgt
    60901 tctatatgac gtaccattgt tttaaaattc agcaatccac gcggatcctg tcccggcgag
    60961 ccgggtatcg gaatttttcg atcggtcagt aggtgcttca atgcgctgtg cttacgaccg
    61021 ttttttgggg aggaacatcc attctccccc ccccgatacc agttgacgta taaaaatttg
    61081 ataatctttg tttttgctat atctcatttc ttctccgata caaaaatagg ttttcctcct
    61141 acaaatggtt aaaaatacac gcacacgatt aaaaaaaacg taaccgcatg actttcaaga
    61201 aaaatcccac tgccgtacag acgtacggaa cagagggatt ggcggcttca agccggattg
    61261 atctaaaaag gaacgtatca gcgttctatt taaaatgctt tggtattaaa tagcaacaaa
    61321 gcatttaaaa gatgggaata ccaattaaca tgaccggtca tgtgtattag tgtcttgctg
    61381 agattcacca aactttacta gtttaaatat gccaaacaac atgacggcaa tcaaaaatag
    61441 taagctgtag gtcatgatca tggtttttcc ctttccttgg gttcctgttt gacaatcttt
    61501 gattcatatt tatccaagta aaagataaat acttgagcta catagaatga accgcgttaa
    61561 cggtttttgt gttcttccga catacgcgaa gtttcaagct gcttggtaat ccaatagccg
    61621 aaagcaagca ccaggaagag aataaatagc agaccgccta tcagcatgac gtatcctttc
    61681 tgtttaatga atcacggtat ttccgtagtt tgtcagcata tacttgcaca aagtaggcaa
    61741 ccaaagccaa tacgactacg ccggaagcac gttcggtcaa agagtatgaa ttgagccaaa
    61801 acaacggcag cattgccaac aaggctgttt tacctatatc ttccagcaat ttgatccaag
    61861 attcaatgct ttccttgtta aaaggaaggt cgggtttgat gaatataaaa aatttcacat
    61921 cggtatctcc atgaaaaagg gagatatacc gatgccctgt ggggatacgg tatatcccca
    61981 tagggttaaa gaaagttgaa aaagagccgt ccgtcccgaa ggacgtgcgc aatgggtaac
    62041 ggcagtgcgc gggcagataa ggtctgccag cctgtgaaat tccaccttac gggctttttc
    62101 agaagacctc aacacgaggt cgtctgaaaa aacccactaa acgtgggtta gatgcaaaaa
    62161 ttgcatcaat cttgtcggtc aaccaagcgt ttggccaact gaacttcaac tgaatcttca
    62221 gcctggttca gaatgtccaa acccgaatcg ggcatatcac cggaggttga ctgagtaacg
    62281 gcaacttttt gccccatcag ttcaaggcaa atttgctgcg ctgtatcgct gtaaccggcg
    62341 aaatacactt ccacatcttc cttctggcca atacgccaag aacggcgggc agcctgcatc
    62401 agggtataaa cgttatatcc tgtttgcaaa aagtagatgg ttggaaatgc caacaagtcc
    62461 aaaccggttt tgaccagctc cggattacag ataacaacct gtacgcctga agccaattgg
    62521 tcttcaaccc agtcttcacg ttgatcggct ttgaccgtcg ctttcattac cgccgcctta
    62581 ataccgtgcc gtttgaaaat ctctttcaga cggcctgtcg tatcatgggt atcggtataa
    62641 acagtataga ccagacattt acgtccttta gccagactgt ctttaacgac ttccagcatg
    62701 tcagcttctt tcggtgagat ttcttcttca tcgaactggg gttcggtatg gaaaagaatc
    62761 ttgttttgtc tgcgccagta aacatcttga ggtttgtaac aacaatccgg ccatgacagc
    62821 aaggcactca taacaacgcc ggtaagggta ttgtcacgat tccttaatgc caccttcaaa
    62881 tgctccctca aaacactctc catattttta tagaccgcct gttgatcttc agtcatttca
    62941 accggtcgga aaacttcttt atagccaggt aaaacgcctt cgcccaaatc acgcagctta
    63001 agaaatacgg taataggcaa aacataacgc atgatgccca aaggggaaaa tcccggtgcc
    63061 ttggcggtgc gaacgctgtt acgctctgct tttgagctac tgaatgcgcc attgctgtat
    63121 tcagttccaa gatgtcggac aatatccttc aacacgccat gcttacgcat aaacgccatg
    63181 cttgccgaac ccaaggtatt ccctttgttg taactgaaac cgtcttcaat catcatttgc
    63241 ggccaaagtc gccacaatag aaagaacagg tcttccgcat agccacccat cagcgtaccg
    63301 gttaagcaca ggatcttatt acagcaacgt gccaaaacgc ccatagcttg accttgagcc
    63361 gtaccatagt tcttgtactc gtgaccttca tctactacca acaacccaaa aaagttcttc
    63421 ggtaaatagc gtttgatgaa ctcggtcggc tgatacccgc ctcgtcccag gctaaattct
    63481 gctttaccta agtctcggtc gatacgcgcc gcctgacggt cgtcgaataa aaattctccg
    63541 ttttcatcca tcaaattgga aaacgcctga atgttgtttt ccaagatttc agccagatac
    63601 tgctcaccat acatcccgat gatttttgct gcggttttag gaccgatgcc gggtagggtg
    63661 gtcatggttt taagtacacg atcgtacact gtgctcacaa ctccatcttc actctgttcc
    63721 ttgcggcaca aggtccagag cggttcctta cacggtttgc catgatgatt gcctttgcaa
    63781 aaacggcggt ttttagccag ggcatttttc accccacctt cagtgttata aggcttgttt
    63841 tcctcatcca agatttcaca gccacattta gggcagtaat actcagaaac ggcaaaaccc
    63901 tcagaagcac cttcagaagg tatccagcgg cgacgaacgg catacgaagg cctccaatgg
    63961 tagtccatac gcatccgaac acgccccatc acaaaaaact ccggaacttc gggtgtgcgg
    64021 tcaagggcac ggatttgcaa cagttttgcc aatgtatcgg ctccgttaag aatccatact
    64081 cgggcattgg gtaccgtttt gaggatttct cgtctccact tgtacaccaa atgaggagga
    64141 ctcaatacca aggtacggtg caaacctgct tgatgcgcgg cagcggctgc tgcaatcgac
    64201 atcatggtct tacctgtgcc catttcggca ttaataatgg ctgcgggttt gtcctctacc
    64261 accaattgct gtaatacggc gcgcacaact tcgcgctgcg ccgcaaacaa aggacgttcc
    64321 aggttatcca gcacttcttc aaggagagga taagtttgtc cctgatagac cggttggttt
    64381 tgctcagtaa ctgcgttaaa gagactatcc ccaaactcag caagaaactg tgtcaggggt
    64441 atgtcggaat ccagcagttc agtttccgga gattcctccg gcaatacggc agcttccgta
    64501 cttttttggg cagtctcagc cacttgacgg atgataaagt tgaaatcagt agttatttga
    64561 ttctgacgtt cctgcatctc ggtcacagtt tgatccagct ctgcaaaatc cgggaagagt
    64621 tcgtctattt ttgaaacggg gttatacatc gtatgctcct aaaaaaggac ggagcaaaac
    64681 cccggcgacg ggggattgcc ccgtcagggt tagaaaccgc taccgtatgg tgcggaaaaa
    64741 gggattagcg aagattcgct aaaggaaaaa atagatgctt ggtggcatct tcgaaagaag
    64801 taaagccgaa atgcctgtaa aagccgatgg cagcttcatc ttttgcctcc acaatcaacg
    64861 catacgcggc gatttctgct ttactgatac gtttgagtgc atcggccaaa agtaaagaac
    64921 ccagtccaaa gccgccatat tttccatcta ccgccaaacg acccatcagc acagcaggaa
    64981 ccgtagggta tctgggtagc ttcttacgaa aaccggcggg tatattactt agggcaactc
    65041 ctgttgcagc caatgtatag tagccaagaa tttgaccatc agatgtaacc gcgacaaaac
    65101 aagtagcgac tttccgtttt acgtcttgcg aaacctgttt ttgtagatag cggtttaaag
    65161 gttcggaagg gcactcaaat gtattacggt catcagcagc atcaagaaga cggattcgga
    65221 agtccggcaa cggcatttta tctcccgaac aattcgtcat gttttttcaa tgctttgctc
    65281 aatgccccat tcggtttgga cggattgagc agggcttggg caaactgaag ttggtcacgt
    65341 tcggacagca tcagaatctc cgcatcggca actgcttttt gggcagcctc gcgggcagca
    65401 ctgataacaa attcggacat actgcgtcct tgaagttccg cagcgtgttt aagtaaagca
    65461 cgggtatctg aagtgattct tgcttcaaaa cggcaatcag catatgtggc agtcatgaac
    65521 atactcctaa attatttcta gattgtacgg ctgattaccg tacaaaacaa gatatgaaag
    65581 ggtattttgt agaaaacatc acatgctcca aagtaacgga gatatacgtt cccctgtggg
    65641 gatcgtcgtc cccgcgaggg ttggaaaaga tgtgcggctg cacaaagtcg gaaaatagcc
    65701 gtccgcccct aaggacgtgc gcaatggata acggtagtac gcagttggca gataaggtct
    65761 gtcagcctgt aaacaggtat ttttagacag cctttcttga aggtcatcta aaaatacctg
    65821 ctcgaaagca ggtaaatcat atccgaataa ccggacatat cttaagaacc tatcagcata
    65881 gacacttttt ctacaatgaa atcattatcg acataaatat caaactgata gtgaatctct
    65941 ggattgatac aggtagaacc ctgtaaggtc agaaatttgt catttgctgt aaaaaaaaga
    66001 acaaaccctt ttctaccgtt tttattcaaa cacgcatgac tgttgtcata cttacgatag
    66061 acaccgccat gattcttgaa attcaaacca tatttcaaag ccagtgcatc tgttactgca
    66121 accaactttt gttttgaccg ctgatcggta tctgtaaata gtccgacccc aggtatatct
    66181 aaagcagcgg caacagcctc aacctgactt ttatattcag gtatcacgcc ggctagaggc
    66241 ttaccttctg tatcgaacac atcgcaaccg tataagccat ctgtttccaa gattctaatt
    66301 tctagggttt tttcgtttaa atattgcatt ggtgtactcc tataaagggc aggggtacac
    66361 gttcccaagg cagggtacgg tatccctgca ggggtagatt gaactgtctg catctgtaca
    66421 aacagcagga ttaaaaatac agtgctactc ttgtaaagct aattactttt atcagtggca
    66481 ccaagaagat ttggattaat ggtcaaaatg tccgaatcct gatcaattac ccctctgtcc
    66541 ggagagagta aatgggctaa ggcaatcatc aaccaatcca aatcacagtt gttgaatttc
    66601 tcacacaaag catctaaaac ctttgctttg tcctcgtctt ccaaaaatct caaatcatca
    66661 aactcatcct tttcaagtgg attgagttca tgtcgagcaa ctgctgtcat catacctaca
    66721 aactcaaatg ctgcttgcaa gtcttcttgc tcaggtttca tgatagtcat agtcatctcc
    66781 ttttcagccg gcttcccggt tcatctcaca cactatttgt cgcaattgct cgctttcacc
    66841 atatttacgg taatgagcaa gtattgcttc aaaaattgca taatgttttt gatcaagctg
    66901 agaaacttgg tataactcaa ctggtactcc cttatagagc gatctaacta tatttcgcaa
    66961 tatctgggag ccagcataac tgtactggcc aagcaaaatt tcgcgtacat cttgctgttg
    67021 aaatacattt tcaggcctat aaatagcatc tgtacaagca ttgtttgtat caaacttcat
    67081 aacaatctcc aaaaataggg gaatatcccc tattggagat gtatccccat agggttgata
    67141 aaagaaaaag ccgtacatcc cgaaggatgc gtgtcatgtt taacggcagg acacaagaca
    67201 gataaggtct gtcagcctgt aaagacaggt atttttagaa gaccttactt gaaggccgtc
    67261 tgaaaatacc tgcccgaagg caggtataat aggacgtaat acgtcaggta ttactagaaa
    67321 cattcttctt gataccaagc caaaatttgg ttgctttcat aatatatcct ccttatgctg
    67381 cttttttcga gtaccaagtt tcgccgttac tgaaacgggc agatacctga tactcctcgc
    67441 accaaggggc gataagttcc tcatagcgtt gccaaagcac ctgttccaag gcgcacaacc
    67501 tgcggcctat gcccttttga tggggataga gctgccgttt tgttgctata tcggcatctt
    67561 ccaactcccc gatcacgtaa tccaccgcct cctcaaacga ggatgtgtca tatccgtcta
    67621 agatacagta attgctgtaa tcagtgaaaa tccggaaaga ttggtcaatg ttattaccgt
    67681 cgtagtatcc acaacggacg ataacatcca tttccatgaa gatagataca ccccagaagg
    67741 tcgtttccac tgatatagtg gcaatccttg tgccaccgaa acaacgggta ttgtattcac
    67801 cgttatcatc tgcttcacca gtagttgaat ctactaggaa gccatcttga cgatattttc
    67861 gattaccaga ttcctcggcc cgatatttaa tgccatcgac ggcatcttca caattatggg
    67921 aaaggatttc cccaatttct tcgtaatcat ctgctttgag acaatctacc tcatccattt
    67981 ccttgttgta ggcttcaaga tcaaaagtgc tgaatgacgt accactgatg acatcaagtt
    68041 tgtggttgta taaaaaatta gaggttccca tggtatttcc tttaaaaaaa ggggatacca
    68101 tagccctttg gggatggtat ccccaaaggg ttagatagat attccaaaat ggaacagttc
    68161 gaaattaaaa aatgctgcaa ccatacgaat acggtcggat aacctcagca gcaatatcca
    68221 tcaacggaat atacagtccg tcatctgtgt aaaactctta tgtaacagac tttttcagaa
    68281 agccttttca aggccatctg aaaaagcccg ctaggggctt cagattaagt atgtaagcgg
    68341 ccgctttgta gcatagcggt caaacgcaca tcaaaatctt ccgggatatc aactcgtacc
    68401 gcttgaactc caactactgc gtactcttca tctatacgag gagtaaaccg catgatggat
    68461 ttatcagcct caagtaacga taaaacagca ttctgccaat gttccaataa cggtatatcg
    68521 gagagttgct gcaccagttg ccaagctgcc ttgtccaagt ctgcggtaac atcatccaac
    68581 ataacccaac ctgttttact atcaaagtta ggttgaatca gaatatccga atacaaaaac
    68641 atgtgagtga ccgaaccata attaagagtc tgatatttcg tcatgaattt gctcagattc
    68701 aagcaatact cggcagttgt caggcgcgga cagcttaaaa cctcctctgg ctgtctaaaa
    68761 ccaagccgtt cattacttac ttgcagttga gcttggaagg acaacatgtc tgcatcgcgg
    68821 ccgtgcagag aagcaaacat caattgtcgg ttccaaatga agaaggcatc ggccatcaaa
    68881 tcattgcgtg aggttaaata caacattcgc gcctcctaga cataccagcg tttttcacga
    68941 gggttatatg aatagccggc ttcgcgtaca agctcaacgg cacattttaa atcctcacga
    69001 tcggcaagaa tgtattctcc aaagctgata tgagaacatt tgttctcaat catctgctca
    69061 acctgattaa gggtctcagc aaactccgga aattcaatac cattctcaga aactggatca
    69121 aaagcaccac tttcagagac tggatcgaca gtagaagctt gactagactg ttcttctact
    69181 tcagctgata aaccacattc ttgtgcgaaa tctgcaagat gttgccaatc taaagcagcg
    69241 tcgatatatg acgttgctcc acctccccat tcaaaagtct ttaccttaag ctggttcagt
    69301 atgaagtttc cttcataatt acctggttca aactgtttca atattgggtt ttttacatca
    69361 aatgttccaa ttgatgtagc taatttagct gttttgaaag ggccacggcg accctgaatg
    69421 acttgcactg ttaaagtgcc ttggattgtc atagacatgg tatctctcca aaagaaaagg
    69481 gataccggcc acgggcagat atcccgtggt tgggaaaaac aaattagtgc ttaatagcac
    69541 tgtaaatctt atccaaatat actttttggg acatatgcag tcccaatctt gtaaaaatac
    69601 gtttgtcgtt atccaatgcc cgaatagcca tattcaacgc tatggaagcg tttttatgac
    69661 tatgaacatc gttgacgctc ctacaatacc gattgaccgc cttttgggtt cgtattttcg
    69721 aaatacgacc atctgcatca acaagtaaca aatgatgttc ttgtatgcat gagacgacaa
    69781 ctttctgatg agtatgtcgg tgggtgagga tggcaccaat acgtattctt tccggtagta
    69841 aatttggggg aaggatggca cctgaacggt gaaaagatag atttagattc atggtaatac
    69901 tccaaaataa agggtacacc atgcccattc ggggaagtgt accccgatgg gttaaaagaa
    69961 atctgcttca gaaaaaaaca gctgtaccgt acctgaaaca ggcttgcaat caacggctat
    70021 gcaatgacag aaaaaacagt ctgtcagctg tataaaactc ttgaaataca gattctctga
    70081 gccgttctgc aaatatcaga acgtcttaga gaacctgcaa agcaggttca aatggggatt
    70141 accctggcta atgattacct agtccgcacc gacgaagtaa tcagataatg gtctggatag
    70201 taataaaacc gtaacaatta cgaataattg cgcgcactgg ttatacggct atacgcagac
    70261 agaagaaacg gtctgtcaac cggggaagct tacctgaatg acctagggta gggaggggca
    70321 ttcaggtaag ctgcccaaag ggcagctttc agacagttgc ttcaaaagca acaaaaataa
    70381 aaaaattcgg attgttaaag aactgtattt ttttactgat gctcacaata gatatgagca
    70441 tcgataaaaa aacaatattg ctgaaagcca agtctttgag gggacttacc ctttacttca
    70501 ttataattca ggagttgctt ggaaaattaa accgccgttt cgacagaggc ttaattttcc
    70561 aagcaatgag ctggaaaact aagaatgggg aaactcgaaa gcttcctacc attcttaaat
    70621 aaacaaaata ttcaatagct ttctaagaag catcagaatt attttgggtt tcatcttgag
    70681 aggatgattg ataccacata tcgccatcca ctttaatgaa tttgaatttc aacaatcgac
    70741 catcgatagt caaaagttct gagacttctc cggttttatg attggttgct tcaaatttca
    70801 aaggttgaat atcaccggca cggaaaccaa ccacgacagt cgggcgttca cggtaatttt
    70861 tagacagcaa ttgcggaaat tctcctaaca ggcgagcaaa gagctctttc gcttccccac
    70921 caacgatacg aagatcgaaa cgggttttct ctccctcatc acctcgagag gcttgtatcg
    70981 tacaagcata atagttggaa gattttcttc cttgaacttc acgtggccgg tttaaaaaac
    71041 cgataccatt tgcagtcagg ttaaagtagt tttgattagt ttgagtcatg atatatctcc
    71101 aaaaaaacag gggatatatc aatcccaaag gggacagtat atccccaaag ggttaagttg
    71161 cggctttcac ggtagccgaa ctgacttaga agccttaaag gctggctgaa aaagaagtac
    71221 cgtaaattcg acgaatcgaa tctcattcat gaacggctaa atgacgagac ttttaagaaa
    71281 agtcacaaac tacaaaaaca attacaatat attcccgata atttcgtatg tcaatatttt
    71341 ttcgtaaaat ctttaaccgc gccaatgccg aaccctgccg gtatccaaat ggacaaaact
    71401 attatagata ccaataccgc ccacattgta gtatttagcc atttgctcaa gctcgctaat
    71461 gctcaccccc ctcatagtaa agtcaactgc tttaccgtgc atatgaagtg agtttctagc
    71521 agcaccctca atagtcgcat ttcgtcttgg tgttctatat gcactattga tcgtaatcac
    71581 aggatttggc cgtcctgatt gccgtgccca ctcttgcatt gcatacatga ggtttaaaag
    71641 tcctacatca atttgaacca tggcatttcc atctttggca tcacgcatca gccaacaggc
    71701 attgcggtaa gcatcaagat catatccatt ttgctgctga aaaaaacgga tttcatgttt
    71761 ttcgccagta tcggcccgtt tgcaattgat tgtacggtct cgtgaccaaa aatcagccat
    71821 actggttgcc gctactacat ctttaggcaa taacaaaccg gcagcaccga tcgtatatag
    71881 ggcaccaacg ccgacaaatt tttttaaaaa atttcttcta tccatctgaa attccaaacc
    71941 aatgactcca atcaaatttt aagcaacact cagtattgat atgcaaactt tccagtatgc
    72001 tggaagagga caacatcttt gagcttcctc aatccctaac agctacaata tccatcgagc
    72061 acgagccgcg cattttcagt tttttgtaac tctgtatatt cttagaaagt tctttattaa
    72121 aaattgaaaa aattgaatct tggttaaaaa tcaatttaac aaaagtgcca aagagtgata
    72181 tacaataaaa cagatgaaaa gttccggata ttgcttttgc aaaatgtgct ggatcctgta
    72241 acatcatgaa tattcgatta aacgcttcag gattgtcggt gttcaaccac agtaaataca
    72301 agacccccag cggcagggca gtaagcgcaa tattgcaatt cttgcagttc accaggtgaa
    72361 atactcccta cttgaatgcc attgatgtaa atgaataaat ctttattagc gttgtattgc
    72421 tccttgtaat tatggttttc atctagcaca tactctatac aaggatttct gaatattgaa
    72481 tcggacaaat atttaacaaa cctgttcttc tccatataca attttcctca tgcgttatag
    72541 cattttagtg cagcttctac agatggccat atgctattcc ttgcacttat atcacgataa
    72601 gcctcaatat cgatcgtatc tttttgttgg gatacggctt aatcgtcttc aattctttat
    72661 cactcagttt caaacccaat tccggtattt caagtgaggc cagttttatt tctcgatctg
    72721 aagtgtctgc aaagtattta ggaataatat gatagtacat aatagataac ctttagttta
    72781 ttaaaagaac gtgtttctat aatgtcaaaa ccgcttattt gacccattcg aagtgtttac
    72841 attcgggaaa cctactacac ccccaatacc gcttatcagg gttttccttg aatcctctct
    72901 gtctcagacg gccttcctta caggtcggac agacatcgtc ttcatttcga gcgaccgcct
    72961 gtttttttgt tccagctgaa tccgtctgtt ttggcggacg agcttttcct acagcatcct
    73021 ttttcgcctg cccaaccagc tctgtaatca ctttctgctg ataagccata aattcagcca
    73081 aagttaattt gccggcttct atcccagcca aagccagctc ccatttggct gtcgttatcg
    73141 gatcggcaat catagtggga atacgggcaa tcagattacg gcctttttct gtcgcataga
    73201 ttttgccccg tttcttttcc atataaccca tttcaaacaa acgatcgata atggcagcgc
    73261 gagtggcttc cgttcccaag ccttccgtac ttttgagaat ttttctgatc tgttcgtctt
    73321 ctacattctt caaaaagtcc ttcaaagtct gcatctcatc gagtagagaa gcctctgtaa
    73381 agcgcggtgg cggcgtggtc tttttagttt cgggattgga ggaaagcgtg tcgacctcat
    73441 cgttgacttt cagatgtggt agagcaggac cttcatcgat ttgatcgcct tcaaacacgt
    73501 ccttgcccaa aagaaccttc caaccggaaa taaccggtaa ttggctggag gctttaaaca
    73561 aatgtgaaca tgcctcaacc tcaactacag tagagtcata ttcaaaatct ggataaaact
    73621 gcgcgatata acggtctcgt atcattcggt acaccgttaa ttcattctta tccaggactg
    73681 acaaatcaaa attactgacc ttggtcggaa taatcgcatg atgggaatgt ttgtttacct
    73741 ggttatcgtt ccataccctt gattgacgtt caggatcagc tttttcaatc actgaaagca
    73801 tggatttatc gatagtctgt atcgcggcaa atacatcttt cacatcactc atctgcgatt
    73861 ttggcaagta tctgcaaggg gtgcggggat atgacgtaat cttataattt tcgtagaggg
    73921 tttgtgcaat tttgagcact ttagaagccg ataggccaag ttttttaccg gcttcttttt
    73981 gcaaagccga aaggctgtac ggtaacgggg gcggggtact tttacgctct ttatttgagg
    74041 taataagctt gcctgtagat ttctcaagct gtttgacaag tgcctttaca gcctcaaggt
    74101 tggtacagta tccatcagta ttacgccagt tttcaggaat ctgccacagt gtagtaaaca
    74161 ctgacttgtc tgctgtctca aaatctactg aaagggtgta gtagttgtaa ggcacgaaag
    74221 tatcgatctc tttatccctt tggactacaa gctttaaagt cggtgtttga atccgtccga
    74281 tcgacagaat atgttctcgg ccatatccgg cagaataagc agctgtgtag gcacgactca
    74341 ggttcatacc taccagccag tcggcgcgac tacgcgccaa accggcttgg taaaggtttt
    74401 ctttatcctt acccggcatt attgaagata aggctttttt taccgattgg gtatctaggc
    74461 cactcgtcca aaatcgggaa atctttttac ggtagccaca gtagtccaat atttcacggg
    74521 caataacttc cccttcacgg tctgcatcag tagcaatcac tacttcatct gctttggcca
    74581 acaagctttt aatgacttta aactgcttcg ataccttcgg tggtacctgc atctgccaaa
    74641 cttcagggag aatgggcaat gcggagatat ttccaaagtc agcatattgt tcgccataag
    74701 catctggcgc ggcctgtgtc aggatatgac caaatgccca ggtaacaata gtgtctccat
    74761 tctgaaaata accatcatgc ctcccgccaa gaacaccgag cactttaccg atgtccttag
    74821 cttgagatgg tttttcacat aaaaacaaac gcattttaaa atcctaagct ttgcttcagc
    74881 tgcaaaacaa gccaagatgc gatggtcaaa aaagcccaaa ttgccaaagc aattaagcca
    74941 ccataaataa gggtaacaat gaacatatcc ttaaagtcag cccataatgc agcaaaccaa
    75001 cagttcattt cacgaaaaaa agaagtattc ttcgtggcca tatatttaca ttgggatgtc
    75061 atcatcagcg tcttcaacgt gattattgtc ctgagactgc tcacgtttag gacgcaactt
    75121 gatagcttct acacgctcag ttggaacaat gaatacatct tgaacacgca atttacgaac
    75181 gcgaacttca ataccatcac gattggtata agtctcgtaa cgttcttcac cggtaacgaa
    75241 aaccggcata ccgacctgta atattttatg gaggtgggca gcatcacgat tccagtattc
    75301 gcaagaaatc cactccgttg aaaccacttc tgatttacct tcggcattgc gtttgaaatt
    75361 aggtgaggca actgaaaaat tcagtacgat tgaggcttca tcgtttttcg ggcttgctac
    75421 aggacgcaga ttaaattcag caccgagatt gccacgaaca gaaagttgaa ctgacatatt
    75481 tttctccaaa aaaaggattt aaaaatatga cagaaaagac agggtaaaat aacccaatat
    75541 gagattaagg gttcttttat cataactaaa taaatttctc ttgtcaacta gcaactatta
    75601 caaattaaat cttatgctat atctagctaa aatgcccacg gacagtcatc ggcacagacc
    75661 cgaaacgaag ttgacgaaag ccgatgaatt cgccgccggc atccaccact gtaacggggc
    75721 gttacagtcg caaaaaaaac catcacaaaa gtgatggcaa ttgattttaa aggctattaa
    75781 atatcttact taaccatgat attactagga agatttttgc gatccaaatt ttcaattttt
    75841 aaaatttcat caaaaatttc tttcatcgaa aacaattctg ccaagtctcc ggcaatctgc
    75901 ctacctcgac aaaaatactg tctgcaatca aaataaacct tcatatgtaa taaccgttca
    75961 tcaaggctgg cattcgtatt atccaacgc
//
LOCUS       DAXHKX010000002        59493 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHKX010000002 DAXHKX010000000
VERSION     DAXHKX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 59493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59493
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.002"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            60..557
                     /locus_tag="ACFMWZ_000082"
     CDS             60..557
                     /locus_tag="ACFMWZ_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934114.1"
                     /translation="MKVLSGWSGRKMWRVLSALPIGVVFFDLIYGFALNVLQGLDLQR
                     AVPDSESVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRL"
     gene            complement(554..721)
                     /locus_tag="ACFMWZ_000083"
     CDS             complement(554..721)
                     /locus_tag="ACFMWZ_000083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934115.1"
                     /translation="MLIHYKSKETAISTTPPQAAAAKHIDCFSTHPLKNAPYPPALTA
                     PPAFAALPDAV"
     gene            complement(877..1568)
                     /locus_tag="ACFMWZ_000084"
                     /pseudo
     CDS             complement(877..1568)
                     /locus_tag="ACFMWZ_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1874..2431)
                     /locus_tag="ACFMWZ_000085"
     CDS             complement(1874..2431)
                     /locus_tag="ACFMWZ_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934116.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(2676..2834)
                     /locus_tag="ACFMWZ_000086"
     CDS             complement(2676..2834)
                     /locus_tag="ACFMWZ_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934117.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2831..3673)
                     /locus_tag="ACFMWZ_000087"
     CDS             complement(2831..3673)
                     /locus_tag="ACFMWZ_000087"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC5934118.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3908..4828
                     /gene="pyrB"
                     /locus_tag="ACFMWZ_000088"
     CDS             3908..4828
                     /gene="pyrB"
                     /locus_tag="ACFMWZ_000088"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC5934119.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4838..5296
                     /gene="pyrI"
                     /locus_tag="ACFMWZ_000089"
     CDS             4838..5296
                     /gene="pyrI"
                     /locus_tag="ACFMWZ_000089"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC5934120.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5361..6026
                     /locus_tag="ACFMWZ_000090"
     CDS             5361..6026
                     /locus_tag="ACFMWZ_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934121.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDAHTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6089..7306)
                     /locus_tag="ACFMWZ_000091"
     CDS             complement(6089..7306)
                     /locus_tag="ACFMWZ_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC5934122.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7490..7993
                     /gene="def"
                     /locus_tag="ACFMWZ_000092"
     CDS             7490..7993
                     /gene="def"
                     /locus_tag="ACFMWZ_000092"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC5934123.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            8080..9006
                     /gene="fmt"
                     /locus_tag="ACFMWZ_000093"
     CDS             8080..9006
                     /gene="fmt"
                     /locus_tag="ACFMWZ_000093"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC5934124.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9085..10344
                     /gene="rsmB"
                     /locus_tag="ACFMWZ_000094"
     CDS             9085..10344
                     /gene="rsmB"
                     /locus_tag="ACFMWZ_000094"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC5934125.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASTACADAQDLSAWYDGKPFDAVLADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQVLKSGGRMLIATCSVFVEENDGQLQKFLNRHA
                     DAELIESRVLLPNKHQDGFYYALIQKH"
     gene            10310..10909
                     /locus_tag="ACFMWZ_000095"
     CDS             10310..10909
                     /locus_tag="ACFMWZ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC5934126.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10909..13029
                     /locus_tag="ACFMWZ_000096"
     CDS             10909..13029
                     /locus_tag="ACFMWZ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC5934127.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEVGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            13022..14299
                     /locus_tag="ACFMWZ_000097"
     CDS             13022..14299
                     /locus_tag="ACFMWZ_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC5934128.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEVGSPFETVARYFHKNGTPWVSPARVEYLIDMPTELLQKAEGGVLYVGDIAQYSR
                     NIQTGIAFIIGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14390..15577
                     /gene="dprA"
                     /locus_tag="ACFMWZ_000098"
     CDS             14390..15577
                     /gene="dprA"
                     /locus_tag="ACFMWZ_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC5934129.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRTLSGKGIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGSRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15603..16064
                     /locus_tag="ACFMWZ_000099"
     CDS             15603..16064
                     /locus_tag="ACFMWZ_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC5934130.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16136..18442
                     /gene="topA"
                     /locus_tag="ACFMWZ_000100"
     CDS             16136..18442
                     /gene="topA"
                     /locus_tag="ACFMWZ_000100"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC5934131.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNSKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVANPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESNDDEESEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18515..19210)
                     /locus_tag="ACFMWZ_000101"
     CDS             complement(18515..19210)
                     /locus_tag="ACFMWZ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934132.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(19230..19781)
                     /locus_tag="ACFMWZ_000102"
     CDS             complement(19230..19781)
                     /locus_tag="ACFMWZ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934133.1"
                     /translation="MEFLSLYPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19899..20276
                     /locus_tag="ACFMWZ_000103"
     CDS             19899..20276
                     /locus_tag="ACFMWZ_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC5934134.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            20365..20934
                     /gene="rsmD"
                     /locus_tag="ACFMWZ_000104"
     CDS             20365..20934
                     /gene="rsmD"
                     /locus_tag="ACFMWZ_000104"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC5934135.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21057..21308
                     /locus_tag="ACFMWZ_000105"
     CDS             21057..21308
                     /locus_tag="ACFMWZ_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC5934136.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            21405..21488
                     /locus_tag="ACFMWZ_000106"
     tRNA            21405..21488
                     /locus_tag="ACFMWZ_000106"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21439..21441,aa:Tyr,seq:gta)
     gene            21519..21592
                     /locus_tag="ACFMWZ_000107"
     tRNA            21519..21592
                     /locus_tag="ACFMWZ_000107"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21551..21553,aa:Gly,seq:tcc)
     gene            21602..21676
                     /locus_tag="ACFMWZ_000108"
     tRNA            21602..21676
                     /locus_tag="ACFMWZ_000108"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21634..21636,aa:Thr,seq:ggt)
     gene            21725..22909
                     /gene="tuf"
                     /locus_tag="ACFMWZ_000109"
     CDS             21725..22909
                     /gene="tuf"
                     /locus_tag="ACFMWZ_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC5934137.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            22916..22991
                     /locus_tag="ACFMWZ_000110"
     tRNA            22916..22991
                     /locus_tag="ACFMWZ_000110"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22949..22951,aa:Trp,seq:cca)
     gene            23106..23384
                     /gene="secE"
                     /locus_tag="ACFMWZ_000111"
     CDS             23106..23384
                     /gene="secE"
                     /locus_tag="ACFMWZ_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC5934138.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            23386..23922
                     /gene="nusG"
                     /locus_tag="ACFMWZ_000112"
     CDS             23386..23922
                     /gene="nusG"
                     /locus_tag="ACFMWZ_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC5934139.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            24023..24457
                     /gene="rplK"
                     /locus_tag="ACFMWZ_000113"
     CDS             24023..24457
                     /gene="rplK"
                     /locus_tag="ACFMWZ_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC5934140.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            24457..25152
                     /gene="rplA"
                     /locus_tag="ACFMWZ_000114"
     CDS             24457..25152
                     /gene="rplA"
                     /locus_tag="ACFMWZ_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC5934141.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            25382..25882
                     /gene="rplJ"
                     /locus_tag="ACFMWZ_000115"
     CDS             25382..25882
                     /gene="rplJ"
                     /locus_tag="ACFMWZ_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC5934142.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            25940..26311
                     /gene="rplL"
                     /locus_tag="ACFMWZ_000116"
     CDS             25940..26311
                     /gene="rplL"
                     /locus_tag="ACFMWZ_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC5934143.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            26501..30679
                     /gene="rpoB"
                     /locus_tag="ACFMWZ_000117"
     CDS             26501..30679
                     /gene="rpoB"
                     /locus_tag="ACFMWZ_000117"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC5934144.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YGNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            30831..35006
                     /gene="rpoC"
                     /locus_tag="ACFMWZ_000118"
     CDS             30831..35006
                     /gene="rpoC"
                     /locus_tag="ACFMWZ_000118"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC5934145.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            35297..35554
                     /locus_tag="ACFMWZ_000119"
     CDS             35297..35554
                     /locus_tag="ACFMWZ_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934146.1"
                     /translation="MKPRNLFFADCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMPINIG"
     gene            35551..35700
                     /locus_tag="ACFMWZ_000120"
     CDS             35551..35700
                     /locus_tag="ACFMWZ_000120"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC5934147.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKISSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            35879..36250
                     /gene="rpsL"
                     /locus_tag="ACFMWZ_000121"
     CDS             35879..36250
                     /gene="rpsL"
                     /locus_tag="ACFMWZ_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC5934148.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            36368..36838
                     /gene="rpsG"
                     /locus_tag="ACFMWZ_000122"
     CDS             36368..36838
                     /gene="rpsG"
                     /locus_tag="ACFMWZ_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC5934149.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            36857..38962
                     /gene="fusA"
                     /locus_tag="ACFMWZ_000123"
     CDS             36857..38962
                     /gene="fusA"
                     /locus_tag="ACFMWZ_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC5934150.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            39050..40234
                     /gene="tuf"
                     /locus_tag="ACFMWZ_000124"
     CDS             39050..40234
                     /gene="tuf"
                     /locus_tag="ACFMWZ_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC5934151.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            40252..40563
                     /gene="rpsJ"
                     /locus_tag="ACFMWZ_000125"
     CDS             40252..40563
                     /gene="rpsJ"
                     /locus_tag="ACFMWZ_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC5934152.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(40649..40831)
                     /locus_tag="ACFMWZ_000126"
     CDS             complement(40649..40831)
                     /locus_tag="ACFMWZ_000126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934153.1"
                     /translation="MLLPKPSPILPFFGGGASLRKAMDKKYPVLFTKKCRLKILEAQN
                     KPISPTALFFDFVVLP"
     gene            40855..41907
                     /locus_tag="ACFMWZ_000127"
     CDS             40855..41907
                     /locus_tag="ACFMWZ_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFC5934154.1"
                     /translation="MFYSQVNKGKMMSLLNVFKDILNNKELNSEERHDLERAVKILQD
                     THVNILITGATGCGKSSTINALFSIDQATGKSDGLVKEVAKVGVGVDPETMDIAKYEL
                     ENMTIWDSPGLGDGEKADQKHRRNIINKLLEKDSNGNAVIDLVLVLLDGGSRDLGTSY
                     ELINNVIINTLKKSGEGRTDRLLVAINQCDMAMKGRNWDAEAGRPNAALENFLEEKVC
                     STRKRIQEATGVTVNPIYFSAGFKSEDEEQRPYNITRLLRYIITAMPSEKRVIVAEKI
                     NVEEIERDTSRRKQEERQEIDSSIGSALEKILGAAGGVVKDVVSTIASGVGTLIGGAA
                     KAIGGAISTVASFFGW"
     gene            41916..42599
                     /locus_tag="ACFMWZ_000128"
     CDS             41916..42599
                     /locus_tag="ACFMWZ_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFC5934155.1"
                     /translation="MHRYRISEIGQKVTAAGFRHLDVLLVGATGVGKSSTLNALFGEQ
                     KAKVGTGVDPETQLVESYMLNDVLRFWDSAGLGDGKEADRAHRQKLIDVLSKTYTHSD
                     GQWGWIDLVFVILDGSSRDLGTAYDLLRDVILKMIDPDRVIVAINQADMAMKGRYWDK
                     VLHQPQPNLQQFLDEKAESVQKRILEATGLQISKPVYYSAYENYHLKEIMDAVINHIP
                     VCRRKMHTP"
     gene            42818..43462
                     /gene="rplC"
                     /locus_tag="ACFMWZ_000129"
     CDS             42818..43462
                     /gene="rplC"
                     /locus_tag="ACFMWZ_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC5934156.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            43462..44082
                     /gene="rplD"
                     /locus_tag="ACFMWZ_000130"
     CDS             43462..44082
                     /gene="rplD"
                     /locus_tag="ACFMWZ_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC5934157.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            44079..44393
                     /gene="rplW"
                     /locus_tag="ACFMWZ_000131"
     CDS             44079..44393
                     /gene="rplW"
                     /locus_tag="ACFMWZ_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC5934158.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            44399..45232
                     /gene="rplB"
                     /locus_tag="ACFMWZ_000132"
     CDS             44399..45232
                     /gene="rplB"
                     /locus_tag="ACFMWZ_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC5934159.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            45239..45517
                     /gene="rpsS"
                     /locus_tag="ACFMWZ_000133"
     CDS             45239..45517
                     /gene="rpsS"
                     /locus_tag="ACFMWZ_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC5934160.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            45526..45855
                     /gene="rplV"
                     /locus_tag="ACFMWZ_000134"
     CDS             45526..45855
                     /gene="rplV"
                     /locus_tag="ACFMWZ_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC5934161.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            45865..46557
                     /gene="rpsC"
                     /locus_tag="ACFMWZ_000135"
     CDS             45865..46557
                     /gene="rpsC"
                     /locus_tag="ACFMWZ_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC5934162.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            46541..46957
                     /gene="rplP"
                     /locus_tag="ACFMWZ_000136"
     CDS             46541..46957
                     /gene="rplP"
                     /locus_tag="ACFMWZ_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC5934163.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            46957..47148
                     /gene="rpmC"
                     /locus_tag="ACFMWZ_000137"
     CDS             46957..47148
                     /gene="rpmC"
                     /locus_tag="ACFMWZ_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC5934164.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            47148..47411
                     /gene="rpsQ"
                     /locus_tag="ACFMWZ_000138"
     CDS             47148..47411
                     /gene="rpsQ"
                     /locus_tag="ACFMWZ_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC5934165.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            47633..48001
                     /gene="rplN"
                     /locus_tag="ACFMWZ_000139"
     CDS             47633..48001
                     /gene="rplN"
                     /locus_tag="ACFMWZ_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC5934166.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            48013..48336
                     /gene="rplX"
                     /locus_tag="ACFMWZ_000140"
     CDS             48013..48336
                     /gene="rplX"
                     /locus_tag="ACFMWZ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC5934167.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            48346..48885
                     /gene="rplE"
                     /locus_tag="ACFMWZ_000141"
     CDS             48346..48885
                     /gene="rplE"
                     /locus_tag="ACFMWZ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC5934168.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            48888..49193
                     /gene="rpsN"
                     /locus_tag="ACFMWZ_000142"
     CDS             48888..49193
                     /gene="rpsN"
                     /locus_tag="ACFMWZ_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC5934169.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            49209..49601
                     /gene="rpsH"
                     /locus_tag="ACFMWZ_000143"
     CDS             49209..49601
                     /gene="rpsH"
                     /locus_tag="ACFMWZ_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC5934170.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            49615..50148
                     /gene="rplF"
                     /locus_tag="ACFMWZ_000144"
     CDS             49615..50148
                     /gene="rplF"
                     /locus_tag="ACFMWZ_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC5934171.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            50162..50515
                     /gene="rplR"
                     /locus_tag="ACFMWZ_000145"
     CDS             50162..50515
                     /gene="rplR"
                     /locus_tag="ACFMWZ_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC5934172.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            50534..51052
                     /gene="rpsE"
                     /locus_tag="ACFMWZ_000146"
     CDS             50534..51052
                     /gene="rpsE"
                     /locus_tag="ACFMWZ_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC5934173.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            51045..51230
                     /gene="rpmD"
                     /locus_tag="ACFMWZ_000147"
     CDS             51045..51230
                     /gene="rpmD"
                     /locus_tag="ACFMWZ_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC5934174.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            51232..51666
                     /gene="rplO"
                     /locus_tag="ACFMWZ_000148"
     CDS             51232..51666
                     /gene="rplO"
                     /locus_tag="ACFMWZ_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC5934175.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            51678..52988
                     /gene="secY"
                     /locus_tag="ACFMWZ_000149"
     CDS             51678..52988
                     /gene="secY"
                     /locus_tag="ACFMWZ_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC5934176.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            52993..53211
                     /gene="infA"
                     /locus_tag="ACFMWZ_000150"
     CDS             52993..53211
                     /gene="infA"
                     /locus_tag="ACFMWZ_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC5934177.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            53232..53345
                     /gene="rpmJ"
                     /locus_tag="ACFMWZ_000151"
     CDS             53232..53345
                     /gene="rpmJ"
                     /locus_tag="ACFMWZ_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC5934178.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            53411..53773
                     /gene="rpsM"
                     /locus_tag="ACFMWZ_000152"
     CDS             53411..53773
                     /gene="rpsM"
                     /locus_tag="ACFMWZ_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC5934179.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            53793..54188
                     /gene="rpsK"
                     /locus_tag="ACFMWZ_000153"
     CDS             53793..54188
                     /gene="rpsK"
                     /locus_tag="ACFMWZ_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC5934180.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            54208..54828
                     /gene="rpsD"
                     /locus_tag="ACFMWZ_000154"
     CDS             54208..54828
                     /gene="rpsD"
                     /locus_tag="ACFMWZ_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC5934181.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            54854..55840
                     /gene="rpoA"
                     /locus_tag="ACFMWZ_000155"
     CDS             54854..55840
                     /gene="rpoA"
                     /locus_tag="ACFMWZ_000155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC5934182.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            55864..56232
                     /gene="rplQ"
                     /locus_tag="ACFMWZ_000156"
     CDS             55864..56232
                     /gene="rplQ"
                     /locus_tag="ACFMWZ_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC5934183.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            56379..57092
                     /gene="minC"
                     /locus_tag="ACFMWZ_000157"
     CDS             56379..57092
                     /gene="minC"
                     /locus_tag="ACFMWZ_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC5934184.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            57121..57936
                     /gene="minD"
                     /locus_tag="ACFMWZ_000158"
     CDS             57121..57936
                     /gene="minD"
                     /locus_tag="ACFMWZ_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC5934185.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            57940..58203
                     /gene="minE"
                     /locus_tag="ACFMWZ_000159"
     CDS             57940..58203
                     /gene="minE"
                     /locus_tag="ACFMWZ_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC5934186.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            58207..59127
                     /gene="oxyR"
                     /locus_tag="ACFMWZ_000160"
     CDS             58207..59127
                     /gene="oxyR"
                     /locus_tag="ACFMWZ_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC5934187.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPQMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(59124..59315)
                     /locus_tag="ACFMWZ_000161"
     CDS             complement(59124..59315)
                     /locus_tag="ACFMWZ_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934188.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
BASE COUNT        15589 a        13317 c        15623 g        14964 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttgtttt ttgttcggcg gcttgcgtgg tcgggtaaaa
       61 tgaaagtttt gagcggttgg tcgggcagga agatgtggcg ggttttgagt gctttgccga
      121 taggcgtggt gttttttgat ttgatctacg gttttgcgtt gaatgtgttg cagggtttgg
      181 atttgcagcg tgccgtgccg gattcggaaa gcgtgttggc ggttacgccc gatattgcat
      241 tcaacagttt gcagattgtc gccaacggcg gtatggcggc ggtggtctgt ttcgggttgg
      301 cggttgtgtt tttgctcaac cgttcggtgc ggcggcggca ggtgttggaa atcggggtgt
      361 tccggatgtt ggggctggtg gcggtattgg cgttcagtgc gccgtcggtg tgggagtggg
      421 cgaacgcgct gccgctgctg ctgaaaggcg cggacgtggt caatacgggg aatgcgcgtt
      481 atgtgctgac ggctttgtgt atgccctttc cggcggtgtc gtgcgtcatc gggctggtgg
      541 ggcggttcag gctttagacg gcatcgggca gggcggcaaa ggcagggggt gcggtcaagg
      601 cgggcggata gggcgcattt ttcagcgggt gcgtcgagaa gcagtcgatg tgtttggcag
      661 ccgcagcttg ggggggtgta gtgctaatgg cggtttcttt gcttttatag tggattaaca
      721 aaaaccagta cggcgttggc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa
      781 gcactaagtg aatgggtttc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
      841 attactatat aaaataaatg ggcaaaaatc ggtttattat cgtttttgcc gcatttggat
      901 ttgttctacc gtaaaacgtg tttgacgaac gggattctta ttaaaaaaca tctgatttct
      961 aacaaaatca gtattttttg gcacgatggc taaaattttt ccttccattt cgccatcacg
     1021 tgttttccat gcgctcaaga attgtgattt gctcattgag acgtgcccca gcgatggatc
     1081 agccagcaaa acagtttctc cgttaatacc gttcaatacc gaaaaatggt tgtttttacg
     1141 gtattttaaa tacacaatta caggaatttt tagttgtacc aactgttcaa atggcaaagc
     1201 ataaccttgt gcttcaaaac ccagttcggg cattatgcgt tgcatatcat caaaagaagc
     1261 acgcatttgg gttttatcca ttttgtctaa gatttctgct tcagaataat gtctgccata
     1321 aaaattattc agtaacgtgg caatcgaagc cgcgccgcaa gaaaaatcca aatcttgttt
     1381 tactatgccg gaatctcgcc gtgctttcca acttcgtaca tggatgtttt ggtaagaagc
     1441 ggggggtcaa caaacatagg ccaagcaaaa actatatttg gggcgaaacc aatcaaagct
     1501 gcataattta tcaatttata aagatttttt atcataatat gtatacgcgg aataaacgca
     1561 tgaaacataa aaaaataaaa tcatatgcaa ttttattgca tgaataaata tgaataaaat
     1621 agataatgat gggagtaaat acgccatgta ttttggaagt ttaaatttat taataataaa
     1681 ataatttatc gtagcgcaaa taaatcccaa aattggaacg attagaaaaa aaattaatac
     1741 acccatcatc atgcaaactc tatattaata aatagctaac taattctatt gtggaaatta
     1801 gttagctaac taaaagttat taatgattat ttccgagaat tgactgcatt gttggcagca
     1861 ttggccccaa aacctagtgc atgaataccc ggtctccatg ccaaattccc agccaatccg
     1921 cctccggcag cagcagccag ccctgttttg ccgctactcc tgttgccgca ccgattcctg
     1981 ccgcagtagc cgcgccttgc gcagttccta atttaccatg attatacaaa ttagcaccat
     2041 gatactccca tgcacctaat gcaccgccaa aagcagcggc tgcaataatg ggaacaaatt
     2101 caccttgtgt ttctttcatt tcagcctgtg ataattgaat tgctttcaca ttttggctgt
     2161 caaaaacttg gctgtctaaa ttttgcgcca ttacaggtgt aatcatcata gccattacag
     2221 ttgcaatttt cgttgcgctg gtttgcacat aaataggatt agcaaattcg ctttgattgc
     2281 gttcagtgtt gatgtagcta atactgcttt ctagtttgaa tttacccttg tcagtcaata
     2341 attcttccaa acttaaaggt aagtcagcat aagcaatttg gctggcaaca gtcaaaataa
     2401 aatctattag acatttgtgt ttttgcatca tttcgtttga ttttctagtt tttgagaatg
     2461 atacaaagtt tttttacaaa gtaaagagtc actctgaaaa acattttttc attataaatc
     2521 aaaatattga tagaataaat agcgagcatc gattcacggt gtactttagt gcaaagcgta
     2581 caacgcgagc ctgaaccacc agccgcaaca ggaaaagaaa gccgatagag tgcaatgctt
     2641 gccaacgtgc aagcgagctt gcaagaacgc ttggctcaac gagagcaggc aagacagaaa
     2701 gcagaaagca ggataggagc ggtaacgcaa aggtctcggg ctttgatttc gccgtaaacc
     2761 ctgctgccgc cttgtccgga aagggtgcag gcggcgagtg ccgacagggt gcagatgggg
     2821 agggggggtt tcatttgggg tcgcaacgga agtggtatgc gcagatttca aaaccgtttt
     2881 tgaaatacag gcggtgcgcg tcggcacggt cgtggttgac gtggacgttg aggtggattt
     2941 tggttacccc tgtttccgcg ccgattttgc ggacttcttc caaaaggcgc gaggcgtagc
     3001 ctttgcggcg gctttgcggc agggtaacga tgtcatcgat gtggatgtgg cggccgctgg
     3061 cgagggtgca ggcttcgcgg aagccgcaga cggcgacggc attgtgtttg ccttcttcaa
     3121 aaatacccag caggcggtag ccttgggggc gttggacttt gttgatctgt tcggtaaagc
     3181 ggttgatgtc ggtcagggcg gaacgcaaaa cgctcaaggc tgcaaaggcg gtggcggtgt
     3241 cgtccgcgcc gatttcgcgc aaaacgtagg atgcgcccga ggcggtctgt tcctgtgctt
     3301 tctcggcggc gtgtttttct tcgattgcct gtgccagcat gacgtgttcg tcggcagggt
     3361 tgttttgtcc gccctgttcg cgttcttcga gcagggcttt gcagtcgatg acgcgcaggt
     3421 cgttgtcggc ggcaaagtcc atcaggaagc ggaacatttg ggggttgtcg gtttccagtt
     3481 ttttatcgac ggcgacgcag cggatgttgt ccaccagtat ggggcgcacc catttcgaat
     3541 aggaaagcct gtggtctttg gtgaacgagg acagaatgcc gcacaggcgt tccgcccagt
     3601 cgctgggacg gaaaatcttg ccggaactcg ttgtgccgtg gatgacgact tcgtaggggt
     3661 tgcagactaa catggcggct tcctgaaaag aaatgtctgg cgcgattata ccttatgctt
     3721 atgcgggcgt gtttggatat gccgtctgaa aagtacagga ttcgtgcagt aaaactttgc
     3781 ggcggcaaat gtgcgataat acgcggcgta ttgccgcttt tgcgaagctg ttccgcaaac
     3841 atacgggcgg cgtggacgta taaccggata cccgcctgac gcgggttttt tacggaaggg
     3901 ggcaaaaatg cctaatccgc tttacagaca gcacatcatc tcgatttcgg atttgtcgcg
     3961 cgaacagttg gaatgcctgc ttcagacggc attgaagctg aaggcgcatc cgcgcggcga
     4021 tttgttggaa ggcaaactta tcggttcgtg cttctttgaa ccgtccacgc gcacgaggct
     4081 gtcgtttgaa acggcggtgc agcggctggg cggcaaggtc atcggctttt cggacggcgc
     4141 gaataccagt gccaaaaaag gcgaaacgct tgccgatacc gcccgcatca tttccggcta
     4201 caccgacgca atcatccaac gccatcccaa agacggcgcg gcgcgcgtgg cggcggagtt
     4261 ttcgcgcgtc cccgtcatca acgccggcga cggcacgaac cagcacccca gtcagacctt
     4321 gttggatctg gtaacgattt atgaaacgca ggggcgtttg gacaagctca aaatcgccat
     4381 ggcgggcgac ttgaaatacg gacgtaccgt gcattcgctt tgtcaggcgt tgaaacgctg
     4441 gaattgtgaa tttgcctttg tttcgccgcc cagcctagcc atgcccgact atattaccga
     4501 agagttggac gaagccggct gccgataccg tatcctcggc agtttggaag aggcggcgga
     4561 atgggcggac atcctgtata tgacccgcgt ccagcgcgaa cgtttcgatg aacaggaatt
     4621 tgccaaaatc caaggcaaat tcaacctcga agcgtctatg ctcgcccacg ccaaaccgaa
     4681 cctgcgcgtg ctgcaccctt tgccgcgcgt ggacgaaatc catcccgatg tcgatgccac
     4741 gccgcatgcc tattatttcg agcaggcaac caacggcgtt tatgcgcgta tggcgatatt
     4801 gtcgctggtg ttgaacgaag aagtgtgagg aaccgatatg gaaaccccga aactcagtgt
     4861 cgaagccatt gaaaaaggta cggttatcga ccatattccc gccggcaggg ggctgaccat
     4921 cctgcgccag ttcaaacttt tgcactacgg caacgcggta accgtgggct tcaacctgcc
     4981 cagcaaaacc caaggcagca aagacatcat caaaatcaaa ggcgtgtgct tggacgacaa
     5041 agccgccgac cgccttgccc tgtttgcacc cgaagcagtg gtcaacacca tcgacaattt
     5101 caaggttgtg cagaaacggc atttgaacct gcccgacgaa atcgccgaag tgttccgctg
     5161 tccgaacacg aattgcgccg gccacggcga gccggtcaaa agccggtttt atgttaaaaa
     5221 acacaacggg cagacgcggc tgaaatgcca ttactgcgaa aaaacctaca gccgggattc
     5281 ggtggcggaa gcctgacgga ttcccttaaa ccgagcgggc ggcatttcgt ctgccgcttg
     5341 ttttgccaat ctgaaatgga atgatgatgc acgcttctgt ccaaagccgt ttcgcaccga
     5401 tactttatgt tttgattttc tttgccggtt ttttgaccgc gcaaatctgg ttcaatcaga
     5461 aagcctatac tgaagagctg cctccgcttc tgtccgcatt gtccgctgtc gcgctggtgt
     5521 ggctggcgtg ggcgttcgtg tcggcgcgtt caaaggctaa ggcggaaaag ttctaccgcg
     5581 aaaaaatgat acagaacgaa agcatacatc ccgtcctgca cgcttctttg caacacttgg
     5641 aacacaagcc gcaaatgctc gccctgctgg tcaaaaacca cggcaaaggg atggcggaac
     5701 aggtcaggtt caaggcggaa gtgctgcccg acgacgaaga cgcgcacacg attgccgccg
     5761 agttggcaaa aatggatatg tttgcattgg ggacggacgc ggtcgcctcg ggcgaaacct
     5821 atggacgcgt gttcgccgat attttcgagt tgtcggcggc tttggaaggg cgcgcgttca
     5881 aaggaatgtt gaaactgacg gcggaatata aaaacatctt cggcgatgcc tgccgttcgg
     5941 aaacggcgtt ggagttgggc gcgctcaatc aggcgttgca ggagatttca aaaacatcgg
     6001 aaaagtccaa acggatattt tattgaagat ggaaaaatgc cgtctgaaac ggaaggtgtt
     6061 tcagacggca tttttgtcgg atgattaatt attcggagcg gttgaagcca aacttcacgc
     6121 ggctgcggcc ctgatccggt atattgtcca aatcgcgtcc cggattggcg gcggtgtcgc
     6181 ctacggaaat atcggagatg ttttccaaaa tgatggcgga cgacaggtgt tcggaggtgc
     6241 ggtaaaccat tgccaagccc acttcttcgg caggagtgga aatcagctcg acggtatccc
     6301 tgcttttgaa attgttggag aggtcgacct gcatcgtttt cttgcgtttg tagaggctca
     6361 aaaccgtgcc tttgtccaaa ccgtccgcct cgcctttgtc gatggtgatg gtttgaaact
     6421 ggccggcaat ccttgtgcct tcaaacacgg aaacgatttt agcctgaacc gggcgggacg
     6481 gttcgtgcgg catcatgttg aagcggtcgg tgtcttccgg cattttcatc aggtagtcgc
     6541 cctgctgtat ttcggaaatg gcggtttcga ccaccagcgg ctgtatcgaa ggggtgcgca
     6601 gcggggtaat caaaggatgg gtgcgggtat ggtattcgtt gtctttcggc cgttctccag
     6661 cctgtttcga gcgttgttcg aggacagagt cggtatagtc gagggagcgc acgatgccgc
     6721 tgaatgcgac ttcctgcccg aggaatttac ccgtatccgg atcggtgatg tttttattga
     6781 ttcggtaggt caggtagcgg cccggctctt tcaggccttt ggtgtaaacc ctggtgcctt
     6841 tggtgtacag cagcctgcct tccgggcccg agagcaggcg cggcgcggca gcggtttctt
     6901 tgcgggaaac gatttgcggg tgccgcataa agatgcggta gaagttgaca tcgatggcgg
     6961 gaataccgta tccggacact tccttatccg gactcatttt gacgacgggg atgccgtctg
     7021 tctgttccaa gccgaggcgc ggttcgccgt caacgtggcg caacaccaat acctggtccg
     7081 gataaatcag gtcgggattg tggatttgat cccggttcgc gtcccacagg cggccccatt
     7141 gccacgggct gtacaggtat ttgcccgaaa tgccccacag ggtgtcgccc tgtttgaccg
     7201 tgtagcgttc cggcgcgttc gggcgcacct ccaaatttgc cgccaaagtt tgtgttgaga
     7261 atgccatacc tgccgcgcag agcagggtta taatacgacg ttgcataacc gttcccctta
     7321 tctgataaat ttcggtttgt cttgcttgat tgggttggaa aaagcggcgg cagcccctcg
     7381 ggatgtgccg cgtgataaaa aatgttccgc attttaacat cgaattatcc gcaccatcac
     7441 ggtaattatg aaaaacaggc ggcgtatccg ccgaaggaaa gagaaaatta tggctttatt
     7501 gaatatcttg caatatcccg acgagcgtct gcacacggtg gcaaagcctg tcgaacaagt
     7561 cgacgagcgc atccggaagc tgattgccga tatgtttgaa acgatgtacg aatcgcgcgg
     7621 catcgggctg gcggcgacgc aggtcgatgt gcacgagcgc gtggtcgtga tggatttgac
     7681 cgaagaccgc agcgaaccgc gcgtgttcat caaccccgtc atcgttgaaa aagacggcga
     7741 aaccacttac gaagagggct gcctgtccgt gccgggcatt tacgacaccg taacccgcgc
     7801 cgaacgcgtc aaggtcgagg ctttgaacga aaaaggcgaa aagttcacgc tggaggcgga
     7861 cggcttgctg gcgatttgcg tgcagcacga gttggaccac ctgatgggca tcgtgtttgt
     7921 cgaacgcctt tcccaactca agcaggggcg gattaagacc aagctgaaaa aacgtcagaa
     7981 acatacgatt tgaccctttt gccgtgccgt ctgaacgctg caaagttttc agacggcacg
     8041 gtcttgtccg acaattttac gcacacgcag gaacacgcta tgaaagtcat cttcgccggc
     8101 acgcccgatt ttgccgccgc cgccttaaga gccgttgccg ccgccggttt tgaaattccg
     8161 ctggtgctga cccagcccga ccgtccgaaa gggcgcggta tgcaactgac tgccccgccc
     8221 gtcaaacaag ccgcgctgga actcggtttg cgcgtcgaac agcccgaaaa gctgcgcaac
     8281 aacgccgaag ccctgcaaat gctcaaagag gtcgaggcag acgtaatggt ggttgccgcc
     8341 tacggtttga ttctgccgca agacgtgttg gatgtgccga aacacggctg cctcaatatc
     8401 cacgcctcgc tgctgccccg ttggcgcggc gcggcgccga ttcagcgcgc gattgaggca
     8461 ggcgatgccg aaaccggcgt gtgcattatg cagatggaca tcggtttgga caccggcgat
     8521 gtggtcagcg aacaccgcta cgccatccaa ccgaccgata ccgccaacga agtccacgac
     8581 gcgctgatgg aaatcggtgc ggcggcggtt gttgccgatt tgcaacagct tcaaagcaaa
     8641 ggccgtctga acgcggtcaa acagcccgaa gaaggtgtta cttacgccca aaaactaagc
     8701 aaagaagagg cgcgtatcga ttggagcgaa agcgcggcgg ttatcgaacg caaaatccgc
     8761 gccttcaacc ccgtgcctgc cgcgtgggtt gagtatcagg gcaagccgat gaaaatccgg
     8821 cgggcggaag tggtggcgca acaaggcgcg gcaggcgaag tgttgtcctg ttcggcggac
     8881 ggtttggtcg ttgcctgcgg cgaaaacgcg ctgaagatta ccgaattgca gcctgccggc
     8941 ggcaggcgta tgaatatcgc ggcgtttgca gcaggacggc atatcgaagc agggacgaag
     9001 ctgtaaatcc cttcagacgg cattccgatc cgcaaacggg aatgccgcct gaaaccatca
     9061 gttgaagaaa gcgaatcaca taatatgagt atggcacttg cccaaaaact tgccgccgac
     9121 agcattgcgg cggttgccga aggacgcaac cttcaggacg tgttggcgca aatccgcacc
     9181 gcgcatcccg accttatggc gcaggaaaac ggcgcgttgc aggacatcgc ctacggctgc
     9241 cagcgttatt tgggcagttt gaaacatatg ctcgcgcaga tgctgaaaaa gccgattggc
     9301 aatccgcagc tcgaaagcct gcttttggcg gcgttgtacc agctgcatta cacgcgcaac
     9361 gcgccccacg ccgtggtcaa tgaggcggtg gaaagcatcg cgaaaatcgg acgcgggcag
     9421 taccgttcgt ttgccaacgc ggttttgcgc cgctttttgc gcgaacgcga caagcttgtg
     9481 gcttcctgta aaaaagacga tgtagcgaaa cacaacctgc cgctgtggtg ggtggcttac
     9541 ttgaaaaacc attatccgaa acactggcac aacatcgccg ccgcgctgca atcccatccg
     9601 ccgatgactt tgcgcgtcaa ccgccgacac ggcaatgccg aaagctattt ggaaaaactg
     9661 gctgcggaag gtatcgcggc taaggcgttg gacgaatatg cggttacgtt ggaagaagcc
     9721 gtgccggtga accgcctgcc tggtttttca gacggcattg tttcggtaca ggacttcggc
     9781 gcgcagcagg cggcgtattt gttaaacccg aaagacggcg aacggatttt ggacgcgtgc
     9841 gccgcgccgg gcggcaagac ggggcatatc ttggaactgg cggattgccg tgttaccgcc
     9901 ttggacattg atgcaggccg tctgaaacgg gtggaagaca atatcgcgcg tctgggcttt
     9961 cagacggcat cgacggcgtg tgccgatgca caggacctgt cggcatggta tgatgggaaa
    10021 ccgtttgatg ccgtccttgc cgacgtgccg tgtaccgcct cgggcgtggc gcggcgcaat
    10081 cccgacgtga aatggctacg ccgtccgacc gacgcgctca aaaccgcccg ccagcaggaa
    10141 gccctgctag atgcattgtg gcaggtgctg aaaagcgggg gaaggatgtt gatcgctacc
    10201 tgttccgtgt tcgtcgagga aaacgacgga caattgcaaa aattcctcaa ccgccatgcc
    10261 gatgcagaac tgatcgaatc gcgggtgctt ttaccgaaca aacaccaaga tggcttttat
    10321 tacgcgctta ttcaaaagca ttaaacaatg gcttgtgctg ctgccgatgc tttccgtttt
    10381 gccggacgcg gcggcggagg ggatagatgt gagccgcgcc gaagcgagga taaccgacgg
    10441 cgggcagctt tccatcagca gccgcttcca aaccgagctg cccgaccagc tccaacaggc
    10501 gttgcgccgg ggcgtgccgc tcaactttac cttaagctgg cagctttccg ccccgataat
    10561 cgcttcttat cggtttaaat tggggcaact gattggcgat gacgacaata ttgactacaa
    10621 actgagtttc catccgctga ccaaccgcta ccgcgttacc gtcggcgcgt tttcgacaga
    10681 ctacgacacc ttggatgcgg cattgcgcgc gaccggcgcg gttgccaact ggaaagtcct
    10741 gaacaaaggc gcgctgtccg gtgcggaagc aggggaaacc aaggcggaaa tccgcctgac
    10801 gctgtccact tcaaaactgc ccaagccatt tcaaatcaat gcattgactt ctcaaaactg
    10861 gcatttggat tcgggttgga aacctctaaa catcatcggg aacaaataat gcgccgtttt
    10921 ctaccgatcg cagccatatg cgccgtcgtc ctgttgtacg gactgacggc ggcaaccggc
    10981 agcaccagtt cgctggcgga ttatttctgg tggattgttg cgttcagcgc aatgctgctg
    11041 ctggtgttgt ccgccgtttt ggcacgttat gtcatattgc tgttgaaaga caggcgcgac
    11101 ggcgtattcg gttcgcagat tgccaaacgc ctttccggga tgtttacgct ggttgccgta
    11161 ctgcccggcg tgtttctgtt cggcgtttcc gcacagttca tcaacggcac gattaattcg
    11221 tggttcggca acgataccca cgaggcgctt gaacgcagcc tcaatttgag caagtccgca
    11281 ttgaatctgg cggcagacaa cgccctcggc aacgccgtcc ccgtgcagat agacctcatc
    11341 ggcgcggctt ccctgcccgg ggatatgggc agggtgctgg aacattacgc cggcagcggt
    11401 tttgcccagc ttgccctgta caatgccgca agcggcaaaa tcgaaaaaag catcaacccg
    11461 cacaagctcg atcagccgtt tccaggtaag gcgcgttggg aaaaaatcca acgggcgggt
    11521 tcggtcaggg atttggaaag cataggcggc gtattgtacg cgcagggctg gctgtcggcg
    11581 ggtacgcaca acgggcgcga ttacgccttg tttttccgtc agccggttcc caaaggcgtg
    11641 gcagaggatg ccgtcttaat cgaaaaggca agggcgaaat atgctgagtt gagttacagc
    11701 aaaaaaggtt tgcagacctt tttcctggca accctgctga ttgcctcgct gctgtcgatt
    11761 tttcttgcac tggtcatggc actgtatttc gcccgccgtt tcgtcgaacc cgtcctatcg
    11821 cttgccgagg gggcgaaggc ggtggcgcaa ggcgatttca gccagacgcg ccccgtgttg
    11881 cgcaatgacg agttcgggcg tttgaccaag ttgttcaacc acatgaccga gcagctttcc
    11941 atcgccaaag aagcagacga gcgcaaccgc cggcgcgagg aagccgccag acattatctt
    12001 gaatgcgtgt tggaggggct gaccacgggc gtggtggtgt ttgacgaaca aggctgtctg
    12061 aaaaccttca acaaagcggc ggaacagatt ttggggatgc cgcttacccc cctgtggggc
    12121 agcagccggc acggttggca cggcgtttcg gcgcagcagt ccctgcttgc cgaagtgttt
    12181 gccgccatcg gcgcagcggc aggtacggac aaaccggtcc atgtgaaata tgccgcgccg
    12241 gacgatgcca aaatcctgct gggcaaggca accgtcctgc ccgaagacaa cggcaacggc
    12301 gtggtaatgg tgattgacga catcaccgtt ttgatacacg cgcaaaaaga agccgcgtgg
    12361 ggcgaagtgg caaaacggct ggcacacgaa atccgcaatc cgctcacgcc catccagctt
    12421 tccgccgaac gtctggcgtg gaaattgggc gggaagctgg atgagcagga tgcacaaatc
    12481 ctgacgcgtt cgaccgacac catcgtcaaa caggtggcgg cattgaagga aatggtcgaa
    12541 gcattccgca attatgcgcg ttccccttcg ctcaaattgg aaaatcagga tttgaacgcc
    12601 ttaatcggcg atgtgttggc attgtatgaa gccggtccgt gccggtttgc ggcggagctt
    12661 gccggcgaac cgctgatggt ggcggcggat acgaccgcca tgcggcaggt gctgcacaat
    12721 attttcaaaa atgccgccga agcggcggaa gaagccgatg tacccgaagt cagggtaaaa
    12781 tcggaagtgg ggcgggacgg tcggattgtc ctgacggttt gcgacaacgg caaagggttc
    12841 ggcagggaaa tgctgcacaa cgccttcgag ccgtatgtaa cggacaaacc ggcgggaacg
    12901 ggattgggtc tgcccgtggt gaaaaaaatc attgaagaac acggcggccg catcagcctg
    12961 agcaatcagg atgcgggtgg ggcgtgtgtc agaatcatct tgccaaaaac ggtaaaaact
    13021 tatgcgtagc agcgatattt taattgtaga cgacgaaatc ggcatccgcg acctgctgtc
    13081 ggaaatcctg caggacgaag gttattcggt cgcattggcg gaaaacgccg aagaggcgcg
    13141 caagctgcgc catcaggcgc gtccggcgat ggtgctgctg gatatttgga tgcccgattg
    13201 cgacggcatc acccttttga aggagtgggc gaaaaacggg cagcttaata tgccggtcgt
    13261 catgatgagc gggcatgcca gcattgacac agcagtggaa gccaccaaaa tcggtgcgct
    13321 cgactttttg gaaaaaccga tctccctgca aaaactgctg tctgccgtcg aaaacgcgtt
    13381 gaagtacggt gcggcgcaaa ccgaaacggg gcctgtattc gacaagctgg gcaacagtgc
    13441 ggcgattcag gagatgaacc gcgaagtcga agcggcggca aagcgcacgt cccctgtatt
    13501 tttgacgggt gaggtgggtt cgccgtttga aaccgtagcg cgctatttcc ataaaaacgg
    13561 tacgccgtgg gtcagtccgg caagggtcga atatctgatc gatatgccga cggaactgtt
    13621 gcagaaagct gaaggcggtg ttttatatgt gggcgatatt gcccaataca gtcgcaatat
    13681 ccaaaccggc attgccttta tcatcggcaa ggcagaacgc tgccgcgtca gggtggtcgc
    13741 atcgggcagc agggcggcag gttcagacgg cattgcctgc gaggaaaagc tggcggaact
    13801 gctgtcggaa tcggtcgtcc gtattccgcc gctgcgtatg cagcatgaag acattccctt
    13861 cctgatacag gggattgcct gcaatgtggc ggaaagccaa aagattgcgc ctgcctcatt
    13921 cagtgaagag gcacttgccg cattgacccg ttacgactgg ccgggaaatt tcgaccaact
    13981 gcaaagcgtc gttgcaacgc tgttgttgga ggcggacgga caggaaatcg gcgcaggggc
    14041 ggtttcttcc cttttggggc agaatgtgcc tgccgaggga gcggaagata tggtgggcgg
    14101 gtttaatttc aacctgcccc tgcgcgaatt gagggaggag gtggagcggc gttatttcga
    14161 gtaccacatc gcccaagaag gtcagaatat gagcaaagtg gcgcagaaag tcggtttgga
    14221 acgcacgcac ctttaccgca aactcaaaca gctcggcatc ggcgtttcgc gccgggcggg
    14281 ggaaaaaacc gaagaatagg cccggacggc cggttttccg gctgcgggct tttgttttca
    14341 gacggcattt ggtgcaaatg ccgtctgaaa tcgtaagggg acggatttta tgacagagga
    14401 cgaacgtttc gcgtggctgc aattggcgtt tacgccctat atcggcgcgg aaagcttcct
    14461 gctgctgctg cgccgtttcg gcagcgcgtc aaacgccctg tccgcaccgg cggaacaggt
    14521 cgccccggcg gtgcggcaca agcaggcggc cgaggcttgg cgcaatgcgg aaaaacgcgc
    14581 ctcggcgcgg caggcggcag aagcggcgtt ggaatgggaa atgcgggacg gatgccgcct
    14641 gatgctgctt caggatgaag atttccccga aatgctgacg caggggctga ccgcgccgcc
    14701 ggttttgttt ttgcgcggca acgtgcggct gctgcacaag ccttccgccg ccatcgtcgg
    14761 cagccgccat gccacgccgc aggcgatgcg gattgccaaa gatttcggta ggacgttgtc
    14821 tgggaaaggc attcccgttg tgtcgggtat ggcttcgggc atcgataccg ccgcccatca
    14881 gggcgcactg gaggcagaag gcggcaccat cgctgtgtgg gggacgggca tagaccgcat
    14941 ttatccgccg tccaataaaa accttgccta tgaaatcgcc gaaaaagggc tgattgtcag
    15001 cgagttcccc atcggtacgc ggccgtatgc cggcaacttt ccgcgccgca accgcctgat
    15061 tgccgcgctg tcgcaggtta cgctggtggt tgaagctgcg ttggaatccg gttcgctgat
    15121 taccgccggc ttggcggcag aaatggggcg cgaagtgatg gcggtacccg gttcgataga
    15181 caatccgcac agcaaaggct gccacaaact gattaaagac ggcgcgaaat tggtggaatg
    15241 cctggacgac atcctgaacg aatgcccggg gctattgcaa aatacgggtg cttcatcata
    15301 ttctataaat aaggatacgc ccgacacggg cagtcgcgcc gttcagacgg catacgcccc
    15361 gccgcctgcc gcaaaaatgc cgtctgaagg ggcggcaggc ggcacagccg tcggcggcat
    15421 actggataaa atgggtttcg accccatcca tcccgacgtg cttgccggac agttggctat
    15481 gcctgccgca gatttgtatg ccgcactgtt ggaattggaa ttggacggca gcgttgccgc
    15541 aatgcccggc ggcagatacc agcgtatccg aacttgaacg cactttatat taaggaacac
    15601 gaatgaccga agtcatcgcc tacctcatcg aacatttcca agatttcgat acctgtccgc
    15661 cgcccgaaga cttgggtatg ctgcttgaag aagcgggttt cgatacgatg gaaatcggca
    15721 acaccctgat gatgatggaa gtattgctca acagctccga attttccgcc gaacctgccg
    15781 acagcggcgc attgcgcgtg tacagcaaag aagaaaccga caacctgccg caggaagtga
    15841 tggggctgat gcagtatctg attgaagaaa aagccgtcag ctgcgaacag cgggaaatca
    15901 tcatccacgc gctcatgcac attccgggcg acgaaattac cgtagatacc gccaaagtgc
    15961 tgaccctgct gcttttatgg gcaaacaaga gcgagctgcc cgtgttggtc ggcgacgagc
    16021 tgatgagcgc gcttttactc gacaacaaac ccacgatgaa ctgaagcggc ttcagacggc
    16081 ccgcccgagg ccgtctgaaa cgtcggcatc aaaaccacca tccagagaac gacaaatggc
    16141 gaaaaaccta ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg
    16201 cggcgatttt gaaatccttg catcctacgg acacgtccgc gaccttgtcc ccaaaagcgg
    16261 cgcggtcgat cccgacaacg gctttgcgat gaaataccaa ttgattagcc gcaacagcaa
    16321 acacgtcgat gccatcgttg ccggtgccaa agaagctgaa aacatctacc tcgccaccga
    16381 cccggatagg gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg
    16441 cggcttgaaa aacatcaagc cgcagcgtgt cgtgttccac gaaatcacca aaaacgccgt
    16501 gctcgatgcc gtcgccaatc cgcgcgaaat cgaaatggac ttggtcgatg cgcaacaagc
    16561 ccgtcgcgct ttggactatt tggtcggctt taacctttcg ccattgttat ggaaaaaaat
    16621 ccgccgcggt ttgagcgcgg gtcgtgtgca aagtcccgct ttgcgcctga tttgcgaacg
    16681 cgaaaacgaa atccgcgcgt ttgaagcgca ggaatattgg acggtgcatc tcgacagcca
    16741 caaaggccgc agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca
    16801 attcgacctg ccgaacgaag ccgcgcaagc cgacgtgttg aaagaactcg aaggcaaaga
    16861 ggccgtcgtt accgccattg aaaagaaaaa gcgcagccgc aatcccgccg cgccgttcac
    16921 cacttctacc atgcagcagg atgctgtgcg caaactcggc ttcaccaccg accgcaccat
    16981 gcgtaccgcc caacagcttt atgaaggtat agacgtaggg cagggcgcga tcggtctgat
    17041 tacctatatg cgtaccgaca gcgtgaattt ggcggatgaa gcgttaaccg aaatccgcca
    17101 ttacatcgaa aacaaaatcg gtaaagaata tctgccgagt gccgccaaac aatacaaaac
    17161 caaatccaaa aacgcccaag aagcgcacga agcgatccgc ccgacttccg tgtaccgcac
    17221 gcccgaaagc gtcaaaccct tcctgagcgc agaccagttc aaactctatc aaatgatttg
    17281 gcagcgcacc gtcgcctgtc agatgacgcc tgccaaattc gaccaaacca ccgtcgatat
    17341 taccgtcggc aaaggcgtat tccgcgtaac cggacaagtg caaaccttcg caggcttcct
    17401 tagcgtttac gaagaaagca acgacgacga agagagtgaa gacagcaaaa aactacccga
    17461 aatgagcgaa ggcgacaaat tgcccgtgga taaactttac ggcgaacaac actttaccac
    17521 tccgccgccg cgctacaacg aagcgacgct ggttaaagcc cttgaagaat acggcatcgg
    17581 ccgcccttcg acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct
    17641 tgagcaaaaa cgcttcatgc ccaccgacac aggcgacatc gtcaacaaat tcctgaccga
    17701 acacttcgcc caatacgtcg attaccactt caccgccaaa ctcgaagacc agcttgacga
    17761 aattgccgac ggcaaacgcc aatggattcc cttgatggac aaattctgga aaccgttcat
    17821 caaacaagtg gaagaaaaag aaggcatcga acgcgccaaa tttaccacgc aggaacttga
    17881 tgaaacctgc ccgaaatgcg gcgaacacaa actgcaaatc aaattcggca aaatgggtcg
    17941 ttttgttgcg tgtgccggtt atcccgagtg cagctacacg cgcaatgtca acgaaaccgc
    18001 cgaagaagct gccgaacgca tcgccaaagc cgaagccgaa caggccgaac tcgacggacg
    18061 cgagtgcccg aaatgtggcg gtcgcctagt gtacaaatac agccgcaccg gcagcaaatt
    18121 catcggctgc gccaactatc cgaaatgcaa acacgtcgag ccgctggaaa aaccgaaaga
    18181 taccggcgtc cagtgtccgc aatgcaaaaa aggcaacctc gtcgagcgca aatcccgcta
    18241 cggcaaactg ttttacagtt gcagcaccta tcccgactgc aactacgcca cttggaaccc
    18301 gcccgttgcc gaagaatgcc cgaactgcca ttggccggtc ttgaccatca aaaccactaa
    18361 acgctggggt gtggaaaaag tctgcccgca aaaagaatgc ggctggaaag aacagatcga
    18421 accgcctgca ccgaaagagt gagatcaggt tggtttgaaa gagaaaaggt cgtctgaaaa
    18481 attttcagac gacctttgct tttctgtgat tggtttattt gaatccgcgt gtcgttttaa
    18541 agtccgataa aatccggttc atttcaggcg caaacaaggc gatgtaatcg taagatagac
    18601 cgcgactggc actgggatgg ggaaagcaga cgacttcgca atcttcaaac gattggaatt
    18661 tgacattgaa acgtgtaccg tcaaattctt tttgcaccgt ctccagcggt ttggtctgct
    18721 taccgaccaa ctgctcgaag cgtggcagta cattttggtt gttcagaaaa tccgccaacc
    18781 tgctgcccat gaagaggatg actttcggac gcagtttttc gatgtggtag agaaaattat
    18841 cgatgtgctc gggttgtgtg aacttgtcgg gattgtcgat agtgttgccc tgtgtagcag
    18901 cccagttggt ttgaaccagg gatttttcaa atgcaccgcc caatccattt tcgtctaagg
    18961 ggtgtcccca catttcaaac caatttttta tcgtattgtc gtaacgccac ttctttgcct
    19021 gctctccgaa atagagggat ttgtttgcaa atgtatggtc gattttgttt tcagggagtt
    19081 tgtattcacc tgctacataa gcagcctcat cggctttact ccaaccccat tcatacccac
    19141 aaatcattaa accatgtttg tcgttgtagc ctttgaacag actgttgctc aaattcaaat
    19201 ccttcatcat gaactcttcc ttttaaaatt taagagcgat tgacttcaat gtttttagat
    19261 ggggtggaaa aatccttgtg taggcaacat aaattcaata aatttcttga taattcgaaa
    19321 cctactaata gcgcacctat aaaagctttt tcattacgtt cagcatgacg gtcacgtcgt
    19381 tcatattttt tacgcttgct gttccctgtt attacagcta agccaagtga tatggcgaga
    19441 attgcccaaa caatagtact taataacaat tttccccata ctatcaataa ggaaagaaaa
    19501 aaccttttag tattagatcg ataggttata atccatgccc atgaaaatgc aataagagtt
    19561 atacataaga tacaagctga caggatttct ttatttttaa ttaaataact tagagcgatg
    19621 aggatgacag gtgtcaagaa aataatagtt acatcccgat agctataaaa gaaaactgcc
    19681 ctattttgaa tgtggagatg tgcacagaat ccaatatagc taaggataat agttaatata
    19741 ataaaaaaag accaccaagg gtaaagagat aggaattcca tgttttcctt tttttgtaaa
    19801 aaggaaaaaa ttttatctaa gagttaattg cctaaccagc cagaagaagt ttaaaatcta
    19861 tcccaataat tcaaccatct atacagaaag ttcagcttat ggaaacccac gaaaaaatcc
    19921 gcctgatgcg cgaattgaat aaatggtccc aggaggatat ggcggaaaag ctggcgatgt
    19981 cggcaggcgg gtatgccaaa atcgaacggg gcgaaacgca gttaaatatc ccgcgtttgg
    20041 agcagttggc tcagattttc aaaatcgata tgtgggactt gctcaaatcg ggcggtggtg
    20101 ggatggtgtt tcagattaat gaaggtgata gtggtggcga tattgcgttg tatgcgtcgg
    20161 gtgatgtttc gatgaaaata gaatttttaa aaatggagtt gaaacactgc aaagaaatgt
    20221 tggaacaaaa agacaaagaa atcgagctgc tccgcaagct gaccgaaacc gtttaaacag
    20281 atatgccgtc tgaaaaaagt tttcagacgg catattcttt gacaggtctt gtataatacc
    20341 gtttgaactt acaggttttt gattatggcg gcaggcaaac ataccaaaca cagcaaccgg
    20401 gtacgcatta tcggcgggca atgccggggc aggaaattga gtttcacatc cgccgacgga
    20461 ctgcgtccga cacccgacag cgtgcgtgaa aagctgttta actggctggg acaggatttg
    20521 acgggtaaaa cggttttgga tctcttcgga ggcagcggcg cactcggtat agaagccgct
    20581 tcgcgcaacg ccaaacgcgt gctgatttcg gataacaacc gccaaaccgt gcagaccttg
    20641 cagaaaaaca gtcgcgaact gggtttgggg caggtgcaaa tcgtctgttc agacggcatc
    20701 gcatatttga agaccgtatc cgaacagttt gatgttgtct ttctcgaccc gccgtttgca
    20761 tggcaggact ggcaaatcct gttcgatgcc ttgaagccgt gcctgaaccc ccgggcattc
    20821 gtctatctcg aggcgggtac gctgccggat attcccgatt ggctgacgga atatagagaa
    20881 gggaaatcag ggcagagtac atttgaatta agggttttcc aagtggctga ataatatgcg
    20941 ctttgataat catttccgag ttgtaaacat tcgtttgcaa ccgtccggtt caaaaaaacc
    21001 ttgtgctata atccgcgccc gcccggtttt gataatttag tggaaaagga aaagaaatgt
    21061 cgctttttat taccgacgag tgcatcaact gcgacgtatg cgaacccgaa tgccccaatg
    21121 atgccatttc ccaaggcgag gaaatttacg aaatcaaccc caacctctgc acgcagtgcg
    21181 tcggacacta cgatgagccg cagtgccagc aggtttgccc ggtggactgc atcctgattg
    21241 acgaagaaca tcccgaaacc catgacgagt tgatggcgaa atacgaaaag attatccagt
    21301 ttaaataaat tctttttaaa acatcaaatt atgtctgttt tgaaataaaa tcaaaaaaaa
    21361 acttgacgga aaagcaagcc gctaataaac taacgttctc ttttggaggg attcccgagc
    21421 ggtcaaaggg ggcagactgt aaatctgttg cgaaagcttc gaaggttcga atccttctcc
    21481 ctccaccaaa attcttactt ggggcagtag cgagtaatgc gggtgtagct caatggtaga
    21541 gcagaagcct tccaagctta cggtgagggt tcgattccct tcacccgctc caaacaatta
    21601 ggcccatgta gctcaggggt agagcactcc cttggtaagg gagaggtcgg cagttcaaat
    21661 ctgcccatgg gcaccatctc tcgattattc atttctttaa ggcttagata tataggatat
    21721 tgccatggct aaggaaaaat tcgaacgtag caaaccgcac gtaaacgttg gcaccatcgg
    21781 tcacgttgac catggtaaaa ccaccctgac tgccgctttg actactattt tggctaaaaa
    21841 attcggcggt gctgcaaaag cttacgacca aatcgacaac gcacccgaag aaaaagcacg
    21901 cggtattacc attaacacct cgcacgtaga atacgaaacc gaaacccgcc actacgcaca
    21961 cgtagactgc ccgggccacg ccgactacgt taaaaacatg attaccggcg ccgcacaaat
    22021 ggacggtgca atcctggtat gttccgcagc cgacggccct atgccgcaaa cccgcgaaca
    22081 catcctgttg gctcgccaag taggtgtacc ttacatcatc gtattcatga acaaatgcga
    22141 catggtagac gatgccgagc tgttggaact ggttgaaatg gaaattcgtg acttgttgtc
    22201 aagctacgac ttcccaggcg acgactgccc aatcgtacaa ggttctgcac tgaaagcttt
    22261 ggaaggtgat gctgcttacg aagaaaaaat cttcgaattg gctgctgcct tggacagcta
    22321 catcccaaca cctgagcgtg ctgtggacaa acctttcttg ttgcctatcg aagacgtatt
    22381 ctctatctct ggtcgtggta cagtagtaac cggtcgcgta gagcgcggta tcatccacgt
    22441 tggtgacgag atcgaaatcg taggtctgaa agaaacccaa aaaaccactt gtaccggcgt
    22501 tgaaatgttc cgcaaactgc tggacgaagg tcaagcaggt gacaacgtag gcgtattgct
    22561 gcgtggtact aaacgtgaag acgtagagcg tggtcaagta ttggctaaac cgggtaccat
    22621 cactcctcac accaaattca aagcagaagt atacgtactg agcaaagaag agggtggtcg
    22681 tcacactcca tttttcgcta actaccgtcc acaattctac ttccgtacta ccgacgtaac
    22741 tggtgcagtt actttggaag aaggcgtaga aatggtaatg ccaggtgaga acgtaaccat
    22801 tactgtagaa ctgattgcgc caatcgctat ggaagaaggt ctgcgctttg cgattcgcga
    22861 aggcggtcgt accgtgggtg ccggcgtggt ttcttctgtt atcgcttaag tttagaggcc
    22921 aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt cgagaccctc
    22981 ttggcctgcc aaataaaaaa ttaaccggcc ttgtgtcggt taattttttt gtatttgtta
    23041 tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta tgatggataa
    23101 ataatatgac agaacatacg cctgaaaaaa agaacgttaa agtggatcaa ctggttgttc
    23161 aagataaaga atctgcatct aattccggta aggaagggtt ttttgcatat ttctcaaatt
    23221 cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgctgtc agaatgactg
    23281 tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca gatacagcaa
    23341 tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc gaaaaaatgg
    23401 tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt ggaagagcgc
    23461 attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt agagaaagtt
    23521 gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc tggttatgtg
    23581 ctagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac cccccgtgtt
    23641 tccggtttta ttggagggag ggctaataga cctacgccga ttagtcagag agaggctgaa
    23701 attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt tgaatttgag
    23761 gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg ggtggttgag
    23821 gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg tagagaaaca
    23881 cccgttgagc tggagttcag ccaggttgaa aagattaact gatttttata cttgaaaaaa
    23941 aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt ccgcgtctta
    24001 cccgttttta ggagttcgtt aagtggcaaa gaaaattatc ggctatatta aactgcaaat
    24061 tcctgcaggt aaagccaatc catctcctcc ggttggtcct gctttgggtc agcgcggttt
    24121 gaatattatg gaattttgta aggcatttaa tgctgcaacc caaggtatgg agcctggctt
    24181 accgattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg tgatgaaaac
    24241 cccgccagct tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta gttctaatcc
    24301 tctgaccaac aaagtgggta aattgacccg tgcccagttg gaagaaattg ctaaaactaa
    24361 agatcctgat ttgactgctg ctgacttgga tgcggctgtc cgtactatag caggttctgc
    24421 tcgctcaatg ggcttggatg tggagggtgt tgtataatgg ctaaagtatc taaacgcttg
    24481 aaagctcttc gctcttctgt ggaagccaat aaattatatg caattgatga agcaattgct
    24541 ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg ttgacgtatc tttcaacttg
    24601 ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt cggtcgttct gcctaaaggc
    24661 accggtaaga taacccgtgt ggctgtattt actcaaggtg caaatgcaga agctgctaaa
    24721 gaagctggtg cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa agcaggcaat
    24781 ctgaactttg acgttgttat tgcttctccc gatgcaatgc gtattgttgg tcaattgggt
    24841 accatcttgg gtcctcgtgg tctgatgcca aaccctaaag taggtacggt tacccctaat
    24901 gttgccgaag cagtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac cgacaaggca
    24961 ggtattgttc atgcaacgat tggtcgtgct tcattcgctg aagctgattt gaaagagaac
    25021 tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa aggtcagtat
    25081 ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga tacatcaagc
    25141 gtaaataact aatcttaagg aattttcaag cagtttggtt ttctgggctg cttgaatttg
    25201 ggctacttaa aattaagtag atgtccaaga ccgtagggat cgtaagattt aatcgtaact
    25261 gccctacgca gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga tgtcttttta
    25321 ggttaccgcg ctggtgggat atcgttttgg tatcctgttt ataaacagtg ggaggtagac
    25381 cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg cggcaattgc
    25441 taatgctcaa accctcgtag tcgctgaata tcgcggtatc agtgtttcca gtatgactga
    25501 gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc gttctgaaaa atactttggc
    25561 tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc gatcaaatgg ttggtccgtt
    25621 ggtttacgct gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc aattcgcgaa
    25681 aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa tgaatgctgc
    25741 tcaggttgct gagttggctt ctattccgag ccgcgaagag ctgttgtcca aactgttgtt
    25801 cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg gctgctttgg cagagaaaaa
    25861 agccggcgaa gaagccgctt aatcgatttt gtttctgtta atcaattatt ttttaataca
    25921 atatttggag taaaatagca tggctattac taaagaagac attttggaag cagttggttc
    25981 tttgaccgta atggaattga acgacttggt taaagctttt gaagaaaaat tcggtgtttc
    26041 tgctgctgct gttgcagttg caggtcctgc tggtgccggt gctgccgatg ctgaagaaaa
    26101 aaccgaattt gatgtcgttt tggcttctgc cggcgatcaa aaagtcggcg tgattaaagt
    26161 tgtccgtgca attaccggtt tgggtctgaa agaagctaaa gacatcgttg acggcgcacc
    26221 taaaaccatt aaagagggtg tttctaaagc tgaagccgaa gacatccaaa aacaactgga
    26281 agaagcaggc gctaaagtcg aaatcaaata atttgatgct tcttatgaag gctggcagtt
    26341 ttctgccagc cttattttgc ttcttaaaat aaacatcaag tattgtttac atttatttgc
    26401 atagttttta tcaagtcatt gcaaataaat gtaaatatca gattgatgcg taccgttgtt
    26461 tcagacggcc tattattgaa aattactttt cggagtgtgt atgaactatt cgtttaccga
    26521 gaaaaaacgt atccgtaaga gttttgcaaa gcgggaaaat gttttggaag ttcctttctt
    26581 gctagcaacc caaattgatt cttatgcgaa gtttttgcag ctggaaaatg cttttgacaa
    26641 acgtaccgat gacggtctgc aggcggcatt taattctatt ttcccgattg tgagccataa
    26701 cggttatgcg cgattggagt ttgtgcatta cacattgggc gagcctttgt tcgatattcc
    26761 cgaatgtcag ttgcgcggaa tcacttatgc agcccccttg cgcgcgcgta tccgtttggt
    26821 gattttggat aaggaagcat ctaaaccgac ggtaaaagaa gttcgtgaaa acgaagtgta
    26881 tatgggcgaa attccgttga tgaccccgag cggttctttt gtgattaacg gcacagagcg
    26941 tgtgattgtt tcccagttgc accgttcgcc cggcgtgttt ttcgagcatg acaaaggtaa
    27001 gacgcactct tccggcaagt tgttattctc cgcccgtatc attccctacc gcggttcatg
    27061 gttggatttt gaatttgacc cgaaagattt gctgtatttc cgtatcgacc gccgccgtaa
    27121 aatgccggta acgattttgt tgaaggcttt gggctacggc aatgagcaaa tcttggatat
    27181 tttctacgac aaagaaacgt tctatttgtc ttcaaacggt gttcaaaccg atttggtcgc
    27241 aggccgtctg aaaggcgaaa ctgccaaggt cgatatcttg gataaagaag gcaatgtatt
    27301 ggttgccaaa ggtaagcgca ttactgcgaa aaatatccgt gatattacca atgcaggcct
    27361 gacccgtttg gatgtagaac cggaaagcct gctgggtaaa gcattggctg ccgatctgat
    27421 tgattcggaa accggcgagg tattggcttc tgccaatgat gaaattacag aagagttgtt
    27481 ggccaaattt gatatcaacg gcgtaaaaga aattacgacc ctttatatca atgagctgga
    27541 tcagggtgct tatatctcca acaccttgcg tacggatgag actgccggcc ggcaggcggc
    27601 gcgtgttgcg atttaccgta tgatgcgtcc gggcgaaccg cccaccgaag aggcggtcga
    27661 gcaattgttt aaccgcttgt tcttcagtga agacagctac gatttgtccc gcgtaggtcg
    27721 tatgaaattt aatacgcgca catacgaaca aaaactgtcc gaagcccaac aaaactcttg
    27781 gtacggccgc ctgctgaacg aaacgtttgc cggtgctgcc gacaaaggcg gttatgtcct
    27841 gagcgtcgaa gatattgtcg cctcgattgc gactttggtc gagttgcgta acggccatgg
    27901 tgaagttgac gatatcgatc acttgggcaa ccgccgagta cgttcggtag gcgagctgac
    27961 tgaaaaccaa ttccgcagcg gtttggcccg tgtggaacgt gctgtaaaag aacgtttgaa
    28021 tcaggcggaa tcagaaaact tgatgccgca cgatttgatt aatgcaaaac ctgtttctgc
    28081 cgctattaaa gaattcttcg gctccagcca attgagtcag tttatggatc agaccaaccc
    28141 cttgtctgaa gtaacccata aacgccgcgt atccgcattg ggtccgggcg gtttgacccg
    28201 cgaacgtgcc ggatttgagg tgcgggacgt gcatccgacc cactacggcc gcgtatgtcc
    28261 gattgaaacg cctgaaggtc cgaacatcgg tttgatcaac tcactgtccg tttatgcgcg
    28321 caccaatgat tacggtttct tggaaacgcc ttaccgccgc gttatcgacg gcaaagtaac
    28381 cgaggaaatc gattacttgt ctgccatcga agaaggccgc tatgtgattg cacaggcgaa
    28441 tgccgatttg gattcagatg gcaatctgat tggcgatttg gttacctgtc gtgaaaaagg
    28501 cgaaaccatt atggcaacgc ccgaccgcgt ccaatatatg gacgtggcaa ccggccaggt
    28561 ggtatccgtt gcggcatccc tgattccgtt cttggaacat gatgacgcga accgcgcatt
    28621 gatgggtgcc aacatgcaac gtcaggcagt gccttgcttg cgtcctgaaa aaccgatggt
    28681 cggtaccggt atcgagcgtt ccgttgccgt tgactctgct actgcaatcg ttgcccgccg
    28741 aggcggcgtg gtcgagtatg tcgatgccaa ccgcgtcgtc gttcgtgttc atgacgatga
    28801 agcgactgcc ggcgaagtgg gtgtcgatat ttacaacttg gttaaattta cccgttccaa
    28861 tcaatctact aacatcaacc aacgtccggc tgtcaaagca ggcgatgttt tgcaacgcgg
    28921 cgatttggtg gccgatggcg cgtccaccga tttgggcgaa ttggctttgg gtcaaaatat
    28981 gaccatcgcc ttcatgccgt ggaacggtta caactacgaa gactcgattc tgatttccga
    29041 aaaagtggct gcggacgacc gctatacttc gattcacatt gaggaattga atgtcgttgc
    29101 ccgcgatacc aagctgggtg cggaagacat tacccgcgat attccgaact tgtccgagcg
    29161 tatgcaaaac cgtttggacg aatccggtat cgtttacatc ggtgcggaag tagaagccgg
    29221 cgatgtgttg gtaggcaagg taacgcctaa aggcgaaacc cagctgacgc cggaagaaaa
    29281 actgctgcgc gccatcttcg gtgaaaaagc atctgacgta aaagatactt cattgcgtat
    29341 gcctaccggc atgagcggta ccgttatcga cgttcaagtc ttcactcgtg aaggtattca
    29401 acgcgacaaa cgtgctcaat ccattatcga ttccgagttg aaacgctacc gtttggattt
    29461 gaacgaccaa ttgcgtattt tcgacaacga cgcattcgac cgtatcgagc gtatgattgt
    29521 cggtcagaaa gccaacggcg gcccgatgaa gctggccaaa ggcagtgaaa tcacgaccga
    29581 atatctggcg ggtctgccga gcaggcacga ttggttcgat atccgtctga ccgatgaaga
    29641 tttggccaag cagttggaac tgattaaagt gagcctgcaa caaaaacgcg aagaagcgga
    29701 cgagttatac gaaatcaaga agaaaaaact gacccaaggc gacgaattgc aacccggcgt
    29761 acaaaaaatg gtgaaagttt ttatcgccat caaacgccgt ctgcaagccg gcgacaaaat
    29821 ggcgggccgc cacggtaaca aaggcgtggt atcgcgcatt ctgccagtgg aagacatgcc
    29881 ttacatggcg gacggccgtc cggtagacat cgtactgaac ccattgggcg taccttcccg
    29941 tatgaacatc ggtcagattt tggaagttca cttgggttgg gcagcaaaag gtatcggcga
    30001 gcgtatcgac cgtatgctga aagagcaacg caaagcaggc gagttgcgcg agttcttgaa
    30061 cagactctac aacggcagcg gtaagaaaga agatttggat gccctgactg atgaagaaat
    30121 catcgaactg gcctccaacc tgcgcaaagg tgcatctttc gcctctcctg tattcgacgg
    30181 tgcgaaagag tctgaaatcc gcgaaatgct gaacttggct tatccgagcg acgatcctga
    30241 ggttgaaaaa ctgggcttca acgacagtaa aacccaaatc acgctgtatg acggccgttc
    30301 aggcgaagca tttgaccgca aggttacagt aggtgtgatg cactatctga aactgcacca
    30361 cttggttgac gaaaaaatgc acgcccgttc taccggtccg tacagtctgg ttacccagca
    30421 gcctttgggc ggtaaagccc agttcggtgg ccaacgtttc ggcgagatgg aggtttgggc
    30481 attggaagca tacggcgcgg catacacgct gcaagagatg ttgactgtga agtctgacga
    30541 cgtgaacggt cgtaccaaaa tgtacgaaaa catcgtcaaa ggcgaacaca aaatcgatgc
    30601 cggtatgccc gagtccttca acgtattggt caaagagatt cgctcactgg gcttggatat
    30661 cgatttggaa cgctactgat acgggtttca gacggcataa ggggagctgt tctgcaggta
    30721 tgcggggcag ccgacaatgt ttaaaaacga aatgccgtct gaaaacactg tacctctatc
    30781 catatcgaaa atccgccatg cggtaaaaat acttccttca aggagcaaaa atgaatttgt
    30841 tgaacttatt taatccgttg caaactgccg gcatggaaga agagtttgat gccatcaaaa
    30901 tcggtattgc ctctcccgaa accatccgct catggtctta tggcgaagtc aaaaaacctg
    30961 aaaccatcaa ctaccgtacg ttcaaacctg agcgtgacgg tttgttctgt gccaaaatct
    31021 ttggcccggt caaagactac gaatgcttgt gcggaaaata caaacgcttg aaatttaaag
    31081 gtgtaacttg tgaaaaatgc ggcgtggaag taaccctgtc caaagtgcgc cgcgaacgca
    31141 tgggccatat cgaattggct gcgcccgtcg cacatatttg gttcttaaaa tccctgcctt
    31201 cccgcttggg tatggtgtta gacatgactt tgcgcgatat cgaacgcgta ttgtactttg
    31261 aagcatttgt ggtaaccgat cccggtatga ctccgctgca acgccgccaa ttgctgactg
    31321 aagacgatta ctacaacaag ctggacgaat acggcgacga tttcgatgcc aaaatgggtg
    31381 cggaaggtat ccgcgaattg ctgcgtaccc tgaatgtagc gggcgaaatc gaaatcctgc
    31441 gccaagagtt ggaatcgacc ggttccgaca ccaaaatcaa aaaaatcgcc aaacgcttga
    31501 aagtattgga agctttccat cgttccggta tgaaactgga atggatgatt atggatgtgc
    31561 tgccggtatt gccgcctgat ttgcgtccgt tggttccatt ggatggtggt cgttttgcca
    31621 cttccgattt gaacgatttg taccgccgcg ttattaaccg taacaaccgt ctgaaacgtc
    31681 tgttggaact gcatgctcct gacatcatcg tccgcaacga aaaacgtatg ttgcaagaag
    31741 cagttgactc gctgttggat aacggccgtc gcggtaaagc catgaccggc gccaacaaac
    31801 gcccgctgaa atcattggca gacatgatta aaggtaaggg tggccgcttc cgtcaaaacc
    31861 tgctgggcaa acgtgtggac tactccggcc gttccgtgat taccgtaggc ccgtacctgc
    31921 gtctgcacca atgcggtttg ccgaaaaaaa tggctttgga actgttcaaa ccgttcattt
    31981 tccacaaatt ggaaaaacaa ggtttggcct ctaccgttaa agcagcgaaa aaattggtag
    32041 agcaagaagt accggaagta tgggacatct tggaagaagt catccgcgaa catccgatta
    32101 tgctgaaccg tgcgccgacc ctgcaccgtt tgggtattca agcgttcgaa cctatcttga
    32161 ttgaaggtaa agcgattcag ttgcacccat tggtgtgtgc tgcgttcaac gccgactttg
    32221 acggcgacca aatggcggta cacgttccat tgagcttgga agcacaaatg gaagcacgca
    32281 cgctgatgct ggcttcaaac aacgtattgt ctcctgccaa cggcgaaccg attatcgtac
    32341 cttcccaaga catcgtattg ggtctgtact acatgacccg cgaccgtatc aatgccaaag
    32401 gcgagggcag cctgtttgcc gatgtgaaag aagtgcatcg cgcataccat accaaacagg
    32461 ttgaactggg tacgaaaatt accgtacgtc tgcgcgaatg ggtgaaaaac gaagcaggtg
    32521 agtttgagcc tgtcgttaac cgttacgaaa caaccgtcgg ccgtgcattg ttgagcgaaa
    32581 tcctgccgaa aggcctgccg tttgaatatg tcaacaaagc gttgaagaaa aaagaaattt
    32641 ctaaactgat taacgcatcg ttccgcctgt gcggcttgcg cgatacggtt atctttgctg
    32701 accacctgat gtacaccggt ttcggatttg cggcaaaagg cggtatttcc attgccgttg
    32761 acgatatgga aattccaaaa gaaaaagcgg ccttgctggc tgaagccaat gccgaggtta
    32821 aagaaatcga agaccaatac cgtcaaggtt tggttaccaa cggcgaacgc tacaacaagg
    32881 tggtcgatat ttggggtcgt gccggcgata aaatcgctaa agcgatgatg gacaacctgt
    32941 ccaaacagaa agttatcgac cgtgccggca acgaagtcga tcaagagtca ttcaactcca
    33001 tctatatgat ggctgactcc ggtgcccgtg gttctgcggc tcagattaaa cagttgtccg
    33061 gtatgcgcgg tttgatggcg aaacctgacg gctcgattat tgaaacgccg attacctcaa
    33121 acttccgcga aggtctgacc gtattgcaat actttattgc gacccacggt gcgcgtaagg
    33181 gtttggcgga taccgcattg aaaaccgcga actccggtta cctgacccgt cgtctggtag
    33241 acgtaactca agatttggtc gttgttgaag acgattgcgg tacttcagac ggctttgtca
    33301 tgaaggcagt ggtacaaggc ggtgatgtga ttgaagcatt gcgcgatcgt attttgggcc
    33361 gtgttaccgc gtctgacgtt gtcgatccgt caagtggcga aacattggtt gaagcaggca
    33421 cgttgttgac tgaaaaactg gtggatatga ttgaccaatc cggtgtcgat gaagtcaaag
    33481 tccgtacgcc gattacttgt aaaacccgtc acggtttgtg cgcgcactgt tacggccgcg
    33541 acttggcgcg cggcaaactg gtcaatgccg gcgaggcagt cggcgtgatt gccgctcagt
    33601 ctatcggcga accgggtacc cagttgacca tgcgcacgtt ccacatcggt ggtgcggcat
    33661 cccgtgcggc agcagccagc caagtggaag ccaaatccaa cggtacggca cgtttcagca
    33721 gccagatgcg ttacgttgcc aacaacaaag gtgagttggt tgtcatcggt cgctcttgcg
    33781 aagtcgtgat tcatgatgac atcggccgtg aacgcgaacg ccacaaagta ccttacggtg
    33841 ccatcctgct ggtacaagac ggtatggcca ttaaagccgg tcaaaccttg gcaacctggg
    33901 atccgcatac ccgtccgatg atcaccgaac atgcaggtat ggtgaaattt gagaacgtgg
    33961 aagagggtgt taccgttgcg aaacaaactg acgatgtaac cggtttgtcc actttggtgg
    34021 tgattgacgg taaacgccgt tccagcagcg cgtccaaact gctgcgtccg actgtaaaat
    34081 tgttggatga aaacggcgtg gaaatctgta ttcccggtac gtccactccg gtttcgatgg
    34141 cattccccgt tggtgcggtg attaccgtac gcgaaggtca ggaaatcggt aaaggcgacg
    34201 tattggcgcg tatcccgcaa gcctcttcca aaacccgcga cattaccggt ggtctgccgc
    34261 gcgttgccga attgtttgaa gcacgcgtgc cgaaagatgc cggtatgttg gcggaaatta
    34321 ccggtaccgt ttccttcggc aaagagacca aaggcaagca acgtctgatt gttactgacg
    34381 tggacggtgt agcatacgag accttgattt ccaaagagaa acaaattctg gtacacgacg
    34441 gtcaagtggt aaaccgcggt gaaaccatcg tggacggagc tgttgatccg catgatattc
    34501 tgcgtttgca aggtatcgaa gcactggcac gctacattgt ccaagaggtg caagaggttt
    34561 accgtctgca aggtgtgaag atttctgata aacacatcga agtcatcatc cgtcaaatgt
    34621 tgcgccgtgt gaacattgcg gatgccggcg aaaccgggtt cattaccgga gagcaggtcg
    34681 aacgcggcga tgtgatggcg gccaatgaaa aagctttgga agaaggcaaa gagccggcac
    34741 gttacgaaaa cgtattgctg ggtattacca aagcctccct gtccaccgac agtttcattt
    34801 ctgccgcatc gttccaagaa acgacccgtg ttctgaccga agccgcgatt atgggcaaac
    34861 aagacgaact gcgcggttta aaagagaacg tcatcgtcgg ccgcctgatt cctgccggta
    34921 ccggtttgac ttatcaccgc agccgtcatc agcaatggca aggggtagag caggagactg
    34981 ctgaaaccca agtaacggat gaataatctt cggcgcgttt attcaataca aaaccgcaag
    35041 ctttaagctt gcggtttttc tttgtccgat taaggcaaaa acaagcgttt tcgtcatttt
    35101 gaggcgtgtg gattattcct taggtatttt cgggccggag accaacgagg tggcgggtgt
    35161 cgtcggtacg tccggagacc aaaataactt tgccaaggat gttggtttcg gcggtcaaaa
    35221 aagtagcgtc ttaatgtttt ccatttaaac aaatgtcgtc tgaaacttca gacggcattt
    35281 cctttaagaa ataaatatga aacccagaaa tctctttttt gcagactgcc tgctgacttc
    35341 ggcgacgttt gccgaggata tcggcgtacc tgtcgaactg attaacgtcg gtaatcggat
    35401 tgcgatgccg tctgaagggg aaagcctcgc cctcctgccg tttgccgagg atgtaccgcc
    35461 ggttcgcgat gcaatgccgt ctgaagttcc taaaagcgcg gcaggcggcg atgttcgggg
    35521 tgaccggata ggaatgccga ttaacatcgg atgagcgcgg ctttatggca taaaaaactg
    35581 tcgtggaaag gatttacacc ccaaataaat ttccgttaca acaagatcag cagcaatatg
    35641 cccgcctttt attcgcgcag cggcaaggaa cggtttgtca gtatagaaaa aacgtattga
    35701 cagtattttc ttcagtcgtc cgactgattg tgagggatgt cggtaaatat ttatcggcaa
    35761 acaagaaaat catctttctt cttgtcgtta tgcttgactg tatacttgca ataaaaatat
    35821 aattccactc ttgccgacat ggtgtcggca agtatttaac tcaacaggac gagaaaatat
    35881 gccaactatc aaccaattgg tacgcaaagg ccgtcaaaag cccgtgtacg taaacaaagt
    35941 gcccgcactg gaagcctgcc cgcaaaaacg cggcgtgtgc acccgtgtat acacgactac
    36001 ccctaaaaaa cctaactctg cattgcgtaa agtatgtaaa gtccgcctga ccaacggttt
    36061 tgaagtcatt tcatacatcg gcggcgaagg ccacaacctg caagagcaca gtgtcgtatt
    36121 gattcgcggc ggtcgtgtaa aagacttgcc aggtgtgcgt taccacactg tacgcggttc
    36181 tttggatact gcaggcgtta aagaccgtaa acaagcccgt tccaaatacg gtgctaagcg
    36241 tcctaaataa ttactgggac ttaaataggc acgtcggccg cctaagctga acaacggccg
    36301 agtaagtgaa tactcaattg ggtattcatg ggaatagacc cgactgaata gattaaagga
    36361 aattaaaatg ccaagacgta gagaagtccc caagcgcgac gtactgccag atcctaaatt
    36421 cggcagcgtc gagttgacca aattcatgaa cgtattgatg attgacggta aaaaatccgt
    36481 tgccgagcgt atcgtttacg gtgcgttgga acagattgag aaaaaaaccg gcaaagtagc
    36541 aatcgaagta tttaacgaag ccattgcaaa cgccaaacct atcgtggaag tgaaaagccg
    36601 ccgtgtaggt ggtgcaaact accaagttcc tgttgaagtt cgtccttcac gccgtttggc
    36661 tttggcaatg cgctgggttc gcgatgcggc ccgcaaacgt ggtgagaaat ccatggacct
    36721 gcgtttggca ggcgaattga ttgatgcgtc cgaaggccgt ggcggtgcgt tgaaaaaacg
    36781 tgaagaagta caccgtatgg ctgaagccaa caaagcattc tctcacttcc gtttctaatt
    36841 ttgaaaggct aataaaatgg ctcgtaagac cccgatcagc ctgtaccgta acatcggtat
    36901 ttccgcccat atcgatgcgg gtaaaaccac gacgacagaa cgtattttgt tctataccgg
    36961 tttgacccac aagctgggcg aagtgcatga cggtgcggct actaccgact acatggaaca
    37021 agagcaagag cgcggtatta ccattacctc cgctgccgtt acttcctact ggtccggtat
    37081 ggcgaaacaa ttccccgagc accgcttcaa catcatcgac accccgggac acgttgactt
    37141 taccgtagag gtagagcgtt ctatgcgtgt attggacggc gcggtaatgg tttactgcgc
    37201 ggtgggcggt gttcaacccc aatctgaaac cgtatggcgg caagccaaca aataccaagt
    37261 gccgcgcttg gcgtttgtca ataaaatgga ccgtcagggt gccaacttct tccgcgttgt
    37321 cgagcaaatg aaaacccgtt tgcgcgcaaa ccctgtacct atcgtcattc cggttggtgc
    37381 ggaagacaac ttcagcggtg tggttgattt gttgaaaatg aaatccatca tttggaatga
    37441 agccgataaa ggtacaacct ttacctatgg cgatattcct gccgaattgg tcgaaactgc
    37501 cgaagaatgg cgtcaaaata tgattgaagc cgcagccgaa gccagcgaag aactgatgga
    37561 caaatactta ggcggcgacg agctgaccga agaagaaatc gtaggcgcgt tgcgtcaacg
    37621 tactttggca ggcgaaattc agcctatgct gtgtggttct gcatttaaaa acaaaggtgt
    37681 tcaacgtatg ttggacgcag ttgtagaatt gctgccagct cctaccgata ttcctccggt
    37741 tcaaggtgtc aacccgaata ccgaggaagc cgacagccgt caagccagcg atgaagagaa
    37801 attctctgca ttggcgttca aaatgttgaa cgacaaatac gtcggtcagc tgacctttat
    37861 ccgcgtttac tcaggcgtag taaaatccgg cgataccgta ttgaactccg taaaaggcac
    37921 tcgcgaacgt atcggtcgtt tggtacaaat gactgccgca gaccgtactg aaatcgaaga
    37981 agtacgcgcc ggcgacatcg cagccgctat tggtctgaaa gacgttacta ccggtgaaac
    38041 cttgtgtgcg gaaagcgcgc cgattatctt ggaacgcatg gaattccccg agccggtaat
    38101 ccatattgcc gttgagccga aaaccaaagc cgaccaagag aaaatgggta tcgccctgaa
    38161 ccgcttggct aaagaagacc cttctttccg tgtccgtaca gacgaagaat ccggtcaaac
    38221 cattatttcc ggtatgggtg agctgcactt ggaaattatt gttgaccgta tgaaacgcga
    38281 attcggtgtg gaagcaaata tcggtgcacc tcaagtggct taccgtgaaa ctatccgcaa
    38341 agccgttaaa gctgaataca aacatgcaaa acaatccggt ggtaaaggtc aatacggtca
    38401 cgttgtgatt gaaatggaac ctatggagcc gggtggtgaa ggttacgagt ttatcgatga
    38461 aattaaaggt ggtgtgattc ctcgcgaatt cattccgtct gtcgataaag gtatccgcga
    38521 tacgttgcct aacggtatcg ttgccggcta tcctgtagtt gacgtacgta tccgtctggt
    38581 attcggttct taccatgatg tcgactcttc ccaattggca tttgaattgg ctgcttctca
    38641 agcgtttaaa gaaggtatgc gtcaagcatc tcccgccctg cttgagccga ttatggcagt
    38701 tgaagtggaa accccggaag aatacatggg cgacgtaatg ggcgacttga accgccgtcg
    38761 cggtgttgta ttgggtatgg atgatgacgg tatcggcggt aaaaaagtcc gtgccgaagt
    38821 acctctggca gaaatgttcg gttactcgac cgacctgcgt tctgcaaccc aaggccgcgc
    38881 tacttactct atggagttca agaaatattc tgaagctcct gctcacatag ctgctgctgt
    38941 aactgaagcc cgtaaaggct aatcaggcaa ataggccgtc cgaaaggctg aaatgatttt
    39001 tcagacggca ttgttcttta atcgatcttt aatgtaaagg aattagctca tggctaagga
    39061 aaaattcgaa cgtagcaaac cgcacgtaaa cgttggcacc atcggtcacg ttgaccatgg
    39121 taaaaccacc ctgactgccg ctttgactac tattttggct aaaaaattcg gtggtgctgc
    39181 aaaagcttac gaccaaatcg acaacgcacc cgaagaaaaa gcacgcggta ttaccattaa
    39241 cacctcgcac gtggaatacg aaaccgaaac ccgccactac gcacacgtag actgtccggg
    39301 gcacgccgac tacgttaaaa acatgattac cggtgccgca caaatggacg gtgcaatctt
    39361 ggtatgttcc gcagccgacg gccctatgcc gcaaacccgc gaacacatcc tgctggcccg
    39421 ccaagtaggc gtaccttaca tcatcgtgtt catgaacaaa tgcgacatgg tcgacgatgc
    39481 cgagctgttg gaactggttg aaatggaaat ccgcgacctg ctgtccagct acgacttccc
    39541 cggcgacgac tgcccgatcg tacaaggttc cgcactgaaa gccttggaag gcgatgccgc
    39601 ttacgaagag aaaatcttcg aattggctgc tgcattggac agctacatcc cgactcccga
    39661 gcgtgccgtg gacaaacctt tcttgttgcc tatcgaagac gtattctcta tttccggtcg
    39721 tggtacagta gtaaccggcc gtgtagagcg cggtatcatc cacgttggtg acgagattga
    39781 aatcgtcggt ctgaaagaaa cccaaaaaac cacttgtacc ggtgttgaaa tgttccgcaa
    39841 actgctggac gaaggtcagg cgggcgacaa cgtaggcgta ttgctgcgcg gtaccaaacg
    39901 tgaagacgtg gaacgcggtc aggtattggc caaaccgggt actatcactc ctcacaccaa
    39961 gttcaaagca gaagtgtacg tattgagcaa agaagagggt ggccgccata ccccgttctt
    40021 cgccaactac cgtccgcaat tctacttccg taccaccgac gtaaccggcg cggttacttt
    40081 ggaagaaggt gtggaaatgg taatgccggg tgagaacgta accattaccg tagaactgat
    40141 tgcgcctatc gctatggaag aaggcctgcg ctttgcgatt cgcgaaggcg gtcgtaccgt
    40201 gggtgccggc gtggtttctt ctgttatcgc ttaattgaag gatattgata aatggcaaac
    40261 caaaaaatcc gtatccgcct gaaagcttat gattacgccc tgattgaccg ttctgcacaa
    40321 gaaatcgttg aaactgcaaa acgtaccggt gccgttgtaa aaggtccgat tccgttgccg
    40381 accaaaatcg aacgtttcaa cattctgcgt tctccgcacg tgaacaaaac ttcccgtgaa
    40441 caattggaaa tccgcaccca cttgcgcctg atggacatcg tggattggac cgataaaact
    40501 accgatgcgc tgatgaagct ggatttgccg gccggtgttg atgtagaaat caaagtccaa
    40561 taattcggac tattgaaaat ccccaagcaa tcaatgcttg gggatttttt atgttatgcc
    40621 gtctgaaaat atgcggcggc aaacagattt aaggcaaaac aacaaaatca aaaaacagtg
    40681 cggtcggcga aatcggctta ttttgtgcct caagaatttt cagacggcat tttttagtga
    40741 acaaaacagg atatttttta tccatagcct tccgaaggct tgcccccccc ccgaagaaag
    40801 gtagaatcgg gcttggtttg ggcagaagca tccatctgat tctaaggatg gatgatgttt
    40861 tattctcaag taaataaagg aaagatgatg agcttactta atgtctttaa agatatttta
    40921 aacaacaaag aattgaattc ggaagaacgg catgatttgg agcgggcggt taagatcttg
    40981 caggatacgc atgtcaatat tttgattacc ggagccaccg gttgcggaaa atcatccacc
    41041 atcaatgcct tgttcagcat agatcaggca acgggaaaga gtgatggact agtaaaggaa
    41101 gtcgcaaaag taggcgtggg tgtagatcct gaaactatgg atattgccaa atatgaattg
    41161 gagaatatga cgatttggga tagtccgggc ttgggcgacg gagagaaggc agaccaaaag
    41221 caccggcgca atatcatcaa taagctgctg gagaaagaca gtaacggcaa tgccgtcatc
    41281 gacttggttt tggtgctgct tgacggcggc tccagagact tggggacatc atacgaactg
    41341 attaacaatg taatcatcaa tacattgaag aagagtgggg aaggccgtac agaccggttg
    41401 ctggttgcga ttaatcagtg tgatatggcg atgaaggggc ggaattggga tgcggaagcc
    41461 ggccgtccga acgccgcctt ggaaaacttt ttggaggaga aagtgtgttc gacacgcaag
    41521 cgcattcagg aagctaccgg cgttacggtc aatccgattt atttttcagc aggctttaag
    41581 agtgaggatg aggagcagcg tccctataac ataacccgcc tgttgcgtta catcattacg
    41641 gctatgccgt ctgaaaaacg ggtgattgtg gcagagaaga ttaatgtgga agaaatcgaa
    41701 cgagatacca gccgccgcaa acaggaggag cggcaagaaa tcgacagtag tatcggcagt
    41761 gcgctggaga aaatattggg ggctgcggga ggcgtggtta aagatgttgt ttcaacgata
    41821 gcaagcggtg tcgggacgct gatcggcggt gctgcaaaag cgattggagg ggcgattagc
    41881 acggttgctt ctttttttgg ctggtaggag cgcatatgca tcgttaccgt atttctgaaa
    41941 tcgggcaaaa agtcacagca gccggtttcc gtcatttgga tgtcttgctt gtcggtgcga
    42001 caggtgtcgg caagtcctca accttaaatg ccttgtttgg cgaacaaaaa gccaaggtag
    42061 gcacaggcgt ggatcccgaa acgcaattgg tcgaaagcta tatgctgaac gatgtgttgc
    42121 ggttttggga cagcgcaggt ttgggcgatg ggaaagaagc cgaccgcgcc catcggcaaa
    42181 aactgattga tgtcctgtct aaaacctata ctcattcgga tgggcagtgg ggctggatag
    42241 atttggtgtt cgttatcctt gacggcagct cccgcgattt gggtacggcc tatgatttgt
    42301 tgagggatgt tatccttaaa atgattgatc cggatagggt tattgttgca atcaatcagg
    42361 cggatatggc gatgaaagga cgttattggg ataaggtgct gcatcagccg cagccgaatt
    42421 tgcaacagtt tctagacgag aaggccgaat cggtacaaaa aaggattctg gaagcaaccg
    42481 gtttgcagat ttccaaaccc gtttactatt cggcatacga aaactaccat ctcaaagaaa
    42541 taatggatgc agtcatcaac catatcccgg tttgtcggag aaagatgcat acgccgtgat
    42601 ggtcggatac cgactccttt aaaagcatcc gaccctatgc cgtctgaaga ttcaaaacgc
    42661 ttcagacggc atattgaaga tatttttgat atttttgttg atatttcttt gacttgtcag
    42721 atataatgtc tgacttggta cattcgtacc aagtttaact ttgtctgaaa gacaggccaa
    42781 tcgtagcctg tccctttact ttaaaaggaa aataatcatg actttaggtc tggttggacg
    42841 caaagttggt atgacccgcg tgttcgacga acagggtgtt tctgttccgg taaccgtttt
    42901 ggatatgtct gccaaccgcg ttacacaagt aaaatccaaa gatactgacg gctatactgc
    42961 cgttcaagtt acctttggtc agaaaaaagc caatcgtgtc aacaaagccg aagccgggca
    43021 ctttgcaaaa gcaggtgttg aagccggtcg cggtttgatt gagtttgctt tgactgaaga
    43081 aaaactggct gaattgaaag ctggtgacga aatcaccgtt tctatgtttg aagtcggtca
    43141 actggtcgat gtaaccggta cctctaaagg taaaggtttc tccggcacga tcaaacgtca
    43201 taacttcggt gcccaacgta cttcccacgg taactcccgt tctcaccgtg ttccaggctc
    43261 tatcggtatg gcgcaagacc cgggtcgcgt gttccccggt aaacgcatgg ccggccaata
    43321 cggcaacacc aaagcaactg ttcaaaaatt ggaagttgtc cgtgttgatg cagaacgcca
    43381 actgctgttg gttaagggtg ctgttccggg tgcggtcaac agcgatgttg tagttcgtcc
    43441 cagcgtgaaa gtaggtgcgt aatggaattg aaagtaattg acgctaaagg acaagtttca
    43501 ggcagcctgt ctgtttctga tgctttgttc gcccgcgaat acaatgaagc gttggttcat
    43561 cagctggtaa atgcctactt ggcaaacgcc cgctctggta accgtgctca aaaaacccgt
    43621 gccgaagtaa aacactcaac caaaaaacca tggcgtcaaa aaggtaccgg ccgcgcccgt
    43681 tccggtatga cttcatctcc gctgtggcgt aaaggtggtc gcgcgttccc gaacaaaccc
    43741 gacgaaaact tcactcaaaa agtaaaccgc aaaatgtacc gtgccggtat ggcgactatt
    43801 ctgtcccaat tgactcgtga cgagcgtttg tttgcgattg aggcgttgac tgctgaaact
    43861 cctaaaacca aagtttttgc tgaacaagtg aaaaatctgg gtctggagca agtgttgttt
    43921 gtaaccaaac agctcgacga gaatgtttac ttggcttcac gcaacttgcc aaacgtgttg
    43981 gttttggaag ctcaacaagt tgatccttac agcttgctgc gttacaaaaa agtaatcatc
    44041 actaaagatg cagttgcaca attagaggag caatgggtat gaatcaacaa cgtttgactc
    44101 aagtgatttt ggcacctatc gtttctgaaa aaagcaacgt attggctgaa aaacgcaacc
    44161 aaatgacgtt taaagtcttg gcaaatgcaa ccaaacctga aatcaaagct gctgttgagc
    44221 tgctgtttgg tgttcaagtt gcttctgtaa ctaccgttac aactaaaggc aaaactaagc
    44281 gttttggtcg tactttgggc cgccgcagcg atgttaaaaa agcttatgta agcttggctg
    44341 ccggtcaaga gttggatttg gaagccgctg ctgcagctgc agataaggaa taaacaaaat
    44401 ggcaatcgtt aaaatgaagc cgacctctgc aggccgtcgc ggcatggttc gcgtggtaac
    44461 agaaggtttg cacaaaggtg caccttatgc gcccttgctt gaaaagaaaa attctactgc
    44521 cggccgtaac aataatggtc atatcaccac ccgtcacaaa ggcggcggtc ataaacacca
    44581 ttaccgtgtt gtagacttta aacgtaacaa agacggcatt cctgccaaag tagagcgtat
    44641 cgaatacgat cctaaccgta ctgccttcat cgcactgttg tgctatgcag acggcgagcg
    44701 tcgttacatc attgctcctc gcggtattca agccggtgct gtattggttt ccggtgctga
    44761 agctgccatc aaagtaggca acaccctgcc gattcgcaac attcccgttg gtacgactat
    44821 ccactgtatc gaaatgaaac ccggtaaagg tgctcaaatc gcacgttctg ccggtgcttc
    44881 tgcggtattg ctggctaaag aaggcgcgta cgctcaagtc cgtctgcgct ctggcgaagt
    44941 ccgtaaaatc aacgtaaatt gccgtgcgac cattggtgaa gttggtaacg aagagcaaag
    45001 cctgaaaaaa atcggtaaag ccggtgccaa ccgttggcgc ggtattcgtc cgaccgtacg
    45061 cggtgttgtc atgaatcctg tcgatcaccc gcatggtggt ggtgaaggcc gtacgggcga
    45121 ggcccgcgaa ccggtcagcc catggggtac tcctgctaaa ggctaccgca ctcgtaataa
    45181 caaacgcacg gataacatga ttgttcgtcg ccgttactca aataaaggtt aatttagtat
    45241 ggctcgttca ttgaaaaaag gcccatatgt agacctgcat ttgctgaaaa aagtagatgc
    45301 tgctcgcgca agcaacgaca aacgcccgat taaaacctgg tctcgtcgtt ctaccattct
    45361 gcctgatttt atcggtctga ccattgctgt gcacaacggc cgcacccatg tgcctgtgtt
    45421 tatcagcgac aatatggttg gtcataaatt aggcgaattc tcattgaccc gtacctttaa
    45481 aggccacttg gccgataaaa aggctaaaaa gaaataaggt gaatcatgag agtaaatgca
    45541 caacataaaa atgcccgtat ctctgctcaa aaggctcgtt tggtagctga tttgattcgt
    45601 ggtaaagacg ttgcccaagc tttgaatatt ttggctttca gtcctaaaaa aggtgccgag
    45661 ctgattaaaa aagtattgga gtcagctatt gctaatgccg agcacaataa cggtgcggac
    45721 attgatgaac tgaaagtggt aactatcttt gttgacaaag gcccaagctt gaaacgtttt
    45781 caagctcgcg ccaaaggtcg cggtaaccgc atcgaaaaac aaacttgtca tatcaatgtg
    45841 acagtgggta actaaggaaa agctatggga caaaagatta accctacagg ctttcgcctg
    45901 gcggtaacta aagactgggc ttcaaaatgg tttgctaaaa gcaccgactt ttctactgtt
    45961 ttgaagcagg atatcgatgt tcgcaattat ttgcgtcaaa aattggccaa tgcttcggtt
    46021 ggtcgagtgg ttattgaacg ccctgcaaaa tctgcacgca ttaccattca ctccgctcgt
    46081 ccgggtgtgg ttataggtaa aaaaggtgag gatatcgagg ttttgaaacg tgacttgcaa
    46141 gtcttgatgg gtgtacctgt tcatgtaaat attgaagaga ttcgccgtcc tgagttggat
    46201 gctcaaatta ttgctgacgg tattgcccag cagttggaaa agcgcgttca attccgtcgt
    46261 gctatgaaac gagcaatgca aaatgcaatg cgttctggtg ctaaaggcat taagattatg
    46321 acttcaggcc gtctgaatgg tgcggatatt gcccgtagcg aatggtatcg tgaaggtcgc
    46381 gtgccactgc atactttacg tgcaaatgta gattatgcaa ccagcgaagc gcacaccaca
    46441 tatggtgtat tgggtctgaa agtttgggtt tatacggaag gcaatattaa atcttccaaa
    46501 cctgaacatg agagtaaaca aagaaaggca ggtagacgta atgctgcagc caactagact
    46561 gaaataccgt aagcaacaaa agggtcgcaa tactggcatc gctactcgcg gtaataaggt
    46621 aagtttcggt gagttcggct tgaaagccgt aggtcgtggt cgtttgactg cccgtcaaat
    46681 cgaagctgct cgtcgtgcaa tgacccgtca tatcaaacgt ggtggtcgta tttggattcg
    46741 tgtattccct gataaaccga ttactgaaaa gcctattcaa gttcgtatgg gtggcggtaa
    46801 aggtaacgtg gaatattaca ttgccgaaat taaaccaggt aaagtgttgt atgaaatgga
    46861 tggcgttcca gaggaactgg ctcgtgaagc attcgagttg gctgctgcca aattgcctat
    46921 tcctacaacc tttgtagtaa gacaggtggg tcaataatga aagcaaatga attgaaagac
    46981 aaatccgttg agcagttgaa tgcagatttg ttggacttgt tgaaagctca gtttggctta
    47041 cgtatgcaaa acgctaccgg tcaattaggc aaaccaagtg aattgaaacg tgtacgtcgc
    47101 gatattgctc gtattaaaac cgttttaact gaaaaaggtg ctaagtaatg agcgaaacta
    47161 aaaatgttcg tactttgcaa ggcaaagtag taagcgacaa aatggataaa accgtaacag
    47221 tattggttga gcgtaaagta aaacatccgc tgtatggtaa gattattcgg ttatctacta
    47281 aaatccatgc ccatgatgaa aataatcaat atggaattgg tgatgtggtt gttatatcgg
    47341 aatcccgtcc attgtcaaaa actaaatctt gggttgtcag tgagctggtt gagaaagcac
    47401 gttctattta agaattaaag caacgtgctt ggaatgggaa acgaagtatt gcagcaaatt
    47461 taatttgcgt gtaaacttcg tttcctgtct ttcagtttct tctggaagtt tcttcccttt
    47521 cggggtccaa gactggttta cttgaaccgc aaggtttcat ttaataagca gcggctttgc
    47581 tgtaagttat ctgaaagtgg taaattaagt tggttaattt aaaggtaata acatgattca
    47641 aatgcagacc atcttagatg tggctgataa ctctggtgcg cgtcgcgtaa tgtgtatcaa
    47701 ggtattgggc ggatctaagc gtcgctacgc ttctgttggc gatattatta aagtggcagt
    47761 taaagatgcg gctccgcgtg gccgtgtcaa aaaaggcgat gtatataatg cggtagttgt
    47821 tcgtactgct aagggtgtac gtcgtcctga tggtgcgtta attaaattcg ataacaatgc
    47881 cgccgtgtta ctgaataata aacttgaacc tttgggtact cgtatctttg gtccggtaac
    47941 ccgtgaattg cgtactgagc gatttatgaa aatcgtttca ttggcacctg aagtattata
    48001 aggaatggca cgatgaataa aatcattaaa ggcgataggg ttgtagtaat tgctggtaag
    48061 gataaaggta agcagggtca agtagttcga gtgttgggtg ataaagttgt tgttgagggc
    48121 gttaatgttg taaaacgcca tcaaaaacct aatccaatgc gtggcattga gggcggtatt
    48181 attactaaag aaatgccttt ggatatttct aatatcgcaa tcctgaatcc ggaaactaat
    48241 aaagcggacc gtgttggtat taagctgatt gaaaatgaag gcaaagttaa acgcgttcgt
    48301 ttcttcaaat caaatggctc tatcattggg gcataaggag ataacatggc tcggttgaga
    48361 gagttttata aagagacagt tgttcctgaa ttggttaaac aatttggtta caaatcagta
    48421 atggaagtcc cgcgtattga aaaaattacc ttgaatatgg gtgtgggtga ggctgttgct
    48481 gataaaaaag ttatggaaca tgctgtttcc gatttagaga aaattgccgg tcaaaaaccg
    48541 gttgttactg ttgcccgtaa atctatcgca ggttttaaaa tccgtgataa ctatccggtt
    48601 ggttgcaaag taacattgcg tcgtgatcaa atgtttgaat tcttggatcg tttgattact
    48661 attgcattac ctcgcgtacg tgacttccgt ggtgtgagcg gtaaatcatt tgatggccgt
    48721 ggcaattaca atatgggtgt tcgtgagcaa attatttttc cggaaattga atacgataaa
    48781 attgatgctt tgcgtggttt gaatattact attactacta cagcaaaaac cgatgaggaa
    48841 gcgaaagctt tattgtcatt gtttaaattt ccgttcaaag gataatcatg gctaagaaag
    48901 cacttattaa tcgtgatctg aaacgtcaag ctttggctaa aaaatatgcg gctaaacgcg
    48961 cggcaattaa agcggtaatc aatgattcga atgcaactga ggaagagcgt tttgaggctc
    49021 gtttgaggtt tcaatccatt cctcgtaatg cggcacctgt gcgtcaacgt cgtcgttgtg
    49081 ctttgacagg tcgccctcgt ggtactttcc gtaaatttgg tttgggtcgt attaaaatcc
    49141 gtgaaatcgc catgcgtggc gaaattccgg gtgttgttaa agccagctgg taataggagt
    49201 aattaagaat gagtatgcat gatcctattt ccgatatgtt gactcgtatc cgcaatgcgc
    49261 aacgtgctaa taaagcagca gttgcaatgc cttcttcaaa attaaagtgt gctattgcaa
    49321 aggtattgaa agaagaagga tatattgagg acttcgcagt ttcatctgac gtaaagtcta
    49381 tattggaaat tcaattaaaa tactatgcag gtcgtcctgt aattgaacaa atcaagcgtg
    49441 tatctcgccc cggtttgcgt atttataaag cgtctagtga gattccaagt gttatgaatg
    49501 gcttgggtat tgctattgtt agtacttcta aaggtgtaat gactgatcgt aaagcacgtt
    49561 ctcaaggtgt tggtggtgag ttgttatgca ttgtagccta gtggaggaaa agaaatgtca
    49621 cgtgtcgcaa aaaacccagt gactgttccc gctggtgtag aagtaaaatt tggaacagag
    49681 gcattagtta ttaagggtaa gaacggtgaa ttgtcttttc ctttgcattc tgatgtagcc
    49741 attgaattta atgatggcaa attgactttt gttgcgaata acagcagtaa acaagcaaat
    49801 gcaatgtctg gtactgctcg cgcattagtc agcaatatgg ttaaaggtgt ttcagaaggt
    49861 tttgagaaaa gattgcaatt gataggtgtg ggttatcgtg ctcaagcaca aggtaaaatc
    49921 ttgaatctgt ctttgggttt ttctcatccg atcgtatatg aaatgcctga aggtgtctcc
    49981 gttcaaactc ctagccaaac agagattgtt ttaaccggct cggataaaca agttgttggt
    50041 caagttgctg ctgagattcg tgcgttccgt gctcctgagc cttataaagg taaaggtgtt
    50101 cgctatgtag gcgaagtagt ggtgatgaaa gaagccaaga aaaaataatt gaggttcact
    50161 aatggataaa catacaaccc gactccgtcg tgcacgcaaa acccgtgctc gtattgcgga
    50221 cttgaaaatg gtaagattat gtgtgttccg aagcaataat catatttatg ctcaagtaat
    50281 tagtgctgaa ggtgataaag tattggctca agcctctaca ttggaagctg aggtgcgcgg
    50341 tagtctgaaa tctggaagca atgttgaagc agctgcaata gttggtaaac gtatcgctga
    50401 gaaggctaaa gcagcaggtg tagaaaaggt tgcttttgat cgttcaggtt tccaatatca
    50461 cggtcgtgtg aaggctttgg ctgaagctgc tcgtgaaaat ggtttaagct tctaaatatt
    50521 tggagacttt cagatggcaa aacatgaaat tgaagaacgc ggtgacggtc tgattgaaaa
    50581 gatggtcgca gttaaccgtg taaccaaagt agttaaaggt ggtcgcatta tggctttctc
    50641 tgcgctaact gttgttggtg atggagatgg tcgtattggt atgggtaaag gtaagtcaaa
    50701 agaagtgcct gttgcagtcc aaaaggcgat ggatcaagca cgacgctcta tgattaaggt
    50761 accattaaaa aatggtacga ttcatcatga ggttattggt cgacatggtg ctactaaagt
    50821 atttatgcag cctgctaaag agggcagtgg cgtaaaagcc ggtggaccta tgcgtttggt
    50881 ttttgatgct atgggcattc ataatatctc cgccaaagtg cacggatcta ctaacccata
    50941 taatatcgta cgtgcaacat tagatggctt gtctaagttg catactcctg ctgatatcgc
    51001 agccaaacgt ggcttgacag tggaagacat tttgggagtt aaccatggct gaacaaaaaa
    51061 agattagggt tacattggtt aaaagcctga ttggtacaat tgaatctcat cgtgcatgtg
    51121 cacgcggttt aggtttgcgt cgtcgcgagc atacggtaga ggttttagat acccctgaaa
    51181 accgtggtat gattaataaa atcagctact tgttgaaagt ggagtcttga tatgtttttg
    51241 aatacaattc aacctgctgt tggtgctacg catgctggtc gtcgtgttgg acgcggtatt
    51301 ggtagtggtc ttggcaaaac gggtggtcgt ggtcataaag gtcaaaagag ccggtctggt
    51361 gggtttcata aggtgggttt cgagggtggt caaatgccct tgcaacgacg cctccctaaa
    51421 agaggtttta aatctttaac agcatcagct aatgcacagc ttcgtttaag tgaactggaa
    51481 tcaattgctg ttaatgagat tgatattttg gtcttaaagc aagcgggtct gattgcatct
    51541 acagtctcta atgttaaagt tattgcttct ggtgaaattt ctaaggcagt tgctttgaag
    51601 ggtattaaag ttaccaaagg tgcgagagct gctatcgagg ctgttggtgg taagattgaa
    51661 atgtaaggtt taatattgtg gctaatcaac aaacgtcatc aggttcatcc aaatttggag
    51721 atcttaagaa acgtcttttg tttctatttg gagcattgat tgtttttcga attggtgccc
    51781 atatacccgt acctggagtt gatgctgttg ctttagctaa attatacgaa agcgctggaa
    51841 acggcatcct gggaatattg aatatgtttt ccggtgggtc gttagagcgc tttagtatat
    51901 ttgcaatagg aattatgcca tatatttcag cttctattat tgtacagctc gcttctgaaa
    51961 ttttgccatc attgaaggct ttaaaaaaag aaggggaggc tggtagaaag gtaattacga
    52021 aatatactag gtatggtact gttttgttag caattcttca aagtctaggt gttgcatctt
    52081 tcgtatttca gcaaggaatt gttgtaacaa gttcatttga gtttcatgtt tccacggtag
    52141 tttctttggt aacgggaacc atgtttctta tgtggcttgg ggagcaaatt actgaaaggg
    52201 gtatcgggaa cggtatttct ttaatcatta cggcaggtat tgcttcaggt attccttcgg
    52261 gtattgcaaa gctggttaca ctgacgaacc aaggttctat gagcatgctt acggcgttgt
    52321 ttattgtatt tggtgcctta ttattaattt atttggttgt atactttgaa agtgcacagc
    52381 ggaagattcc tattcattat gcaaaacgcc agtttaatgg tagggcgggt agtcaaaata
    52441 cgcatatgcc tttcaagttg aatatggctg gtgttattcc cccaattttt gcttccagta
    52501 ttattctatt tccatctact cttttaggtt ggtttggttc ggctgataca aatagtgttt
    52561 tgcacaaaat agctggattg ttacaacacg gtcaattgct gtatatggct ttatttgcag
    52621 cgacagttat tttcttttgt tatttttata cggctttggt ttttagccct aaagaaatgg
    52681 cagagaattt aaaaaagagt ggtgcttttg ttcctgggat tagacctggt gagcagacct
    52741 ctaggtattt agaaaaagtt gtattacgtt tgacattgtt tggagctctt tatattacaa
    52801 ctatttgttt aattccagag ttcttaacta cggttttaaa tgtacctttt tatttgggtg
    52861 gcacgtcttt gttgattcta gttgttgtaa cgatggattt tagtacacaa ataaattcgt
    52921 ataggcttac tcaacagtat gataagttaa tgactcgttc agaaatgaaa tcattttctc
    52981 ggaaatagaa ttatggcgaa agaagatact atccaaatgc aaggtgaaat tcttgaaact
    53041 ttacctaatg caacatttaa agtaaaactt gagaatgacc atattgtatt gggtcatatt
    53101 tctgggaaga tgcggatgca ttacattcgt atttctccgg gagataaggt cacagtagag
    53161 ctgacacctt atgatctaac tagggctcga atcgttttca gagcaagata aaccaataaa
    53221 aggaaaataa aatgcgtgta caaccatctg ttaagaaaat ttgccgaaat tgcaagatta
    53281 ttcgtcgaaa tcgtgtagtt cgtgtaattt gtactgatct ccgtcacaaa cagcgtcaag
    53341 gttaatggaa tatttctttt aatgtgattc tgtgatatag tgacacactt tgccctaaaa
    53401 aggaaaaaat atggctcgta ttgcaggggt aaatatccct aataacgcac acatcgtaat
    53461 tggtcttcag gctatttacg gtattggtgc tactcgtgct aaattgattt gtgaggctgc
    53521 aaatattgcg cctgatacta aagcaaaaga tttggacgag actcaattag atgctttgcg
    53581 tgaccaagtt gccaagtatg aagtagaagg tgatttgcgt cgtgaggtaa ctatgagtat
    53641 caagcgattg atggacatgg gctgctatcg tggcttccgt catcgtcgcg gcttaccatg
    53701 ccgcggtcaa cgcactcgta caaatgcgcg tacccgcaaa ggtccgcgta aagcgattgc
    53761 tggtaagaaa taaattttaa ggaattttat taatggctaa agcaaacaca gcttcacgtg
    53821 tacgtaaaaa agtacgtaaa accgtgagtg agggtattgt gcacgttcat gcatctttca
    53881 acaataccat cattacaatc actgaccgtc aaggcaatgc gttgtcttgg gctacctctg
    53941 gcggcgctgg ttttaaaggt tctcgtaaaa gtacaccatt tgcagcacaa gttgcagcag
    54001 aagcagctgg taaagttgcc caagagtatg gcgttaaaaa tttagaggtt cgtattaaag
    54061 gtccaggtcc aggtcgtgaa tcctctgtac gtgctttgaa tgctcttggt ttcaagatta
    54121 ccagcattac tgacgttacc ccgttgcctc ataacggttg ccgtccgcct aaaaaacgtc
    54181 gtatttaata ttggagtgat ttgaaacatg gcacgttata ttggccctaa atgtaagttg
    54241 gcacgtcgcg aaggtacgga tttgtttttg aagagtgcgc gccgctcttt ggattctaaa
    54301 tgtaaaattg attccgctcc cggtcagcat ggtgcaaaaa aaccgcgttt gtcagactat
    54361 ggtttgcagt tgcgtgaaaa acaaaaaatc cgccgtattt atggcgtatt agaacgtcag
    54421 ttccgtcgtt atttcgcaga agctgatcgt cgtaaaggtt ctaccggcga gttgctgttg
    54481 cagttgctgg aatctcgttt ggataatgtc gtttatcgta tgggtttcgg ttctacccga
    54541 gctgaagcaa gacagcttgt ttctcataag gcgatagttg tgaatggaca agttgtcaat
    54601 attccttctt tccaagtgaa agctggtgat gttgtctcag ttcgtgaaaa agccaaaaaa
    54661 caggtacgta ttcaagaagc attgggtttg gcaactcaaa tcggcttgcc gggttgggtt
    54721 tctgtagatg cggataaact tgagggtgtg ttcaaaaaca tgccggatcg ctcggaattg
    54781 accggtgata ttaatgaaca gctggtggta gagttctact ctaaataatg ctagctcagt
    54841 gagggacagt taaatgcaga atagcacaac cgaatttttg aaacctcgtc aaattgatgt
    54901 aaatactttt tctgcaactc gtgcaaaagt atctatgcag ccatttgaac gtggtttcgg
    54961 tcatacctta ggtaatgctt tgcgccgtat cttactgtca tccatgaatg gttttgctcc
    55021 tactgaagta gctattgccg gtgtattaca cgaatattct actgttgatg gtattcagga
    55081 agatgttgtt gacattttgc tgaatattaa aggtattgtg tttaaactcc atggtcgtag
    55141 ccaagttcaa cttgtgttga agaaatcagg ttcaggtgtc gtatctgctg gtgatattga
    55201 gttgccgcat gatgtagaaa ttctgaatcc tggtcatgtc atttgtcatt tggctgataa
    55261 cggtcaaatt gagatggaaa ttaaagtaga gcaaggtcgt ggttatcaat ctgtttcagg
    55321 tcgtcaggta gttcgtgatg agaaccgtca gattggtgca atccagttgg atgcgagctt
    55381 ttcgcccatc agccgtgtta gctttgaggt tgaacctgca cgtgtagagc agcggacgga
    55441 tcttgataag ttggttttgg atatcgaaac cgacggttct attgatcctg aggaagctgt
    55501 acgcagtgcg gcacgtattt tgattgatca gatgtctatt tttgctgatt tgcagggtac
    55561 tcctgtggag gaggttgaag aaaaagcacc tcctatcgac cctgttcttt tgcgtccggt
    55621 ggatgatctg gaattgacag tacgttcagc taattgtttg aaagctgagg atatttatta
    55681 tattggcgat ttgattcaac gcactgaaac cgagcttctt aaaacgccga atttgggacg
    55741 taaatctttg aatgagatta aggaagtatt ggcatctaaa ggtttgacac tgggttctaa
    55801 gttggaagca tggccacctg taggcttgga aaagccttaa tgaagaatta aaggataatt
    55861 gatatgcgtc atcgtaatgg caatcgcaaa ttaaaccgta ccagcagtca tcgtgctgca
    55921 atgctgcgta atatggcgaa ttcattattg actcacgaaa ctattgtaac aactctgcct
    55981 aaggccaagg aattgcgccg tgtagtagag ccgttgatta cattgggtaa aaagccgtca
    56041 ttggcaaacc gccgtttggc atttgaccgt actcgcgacc gtgatgttgt agtaaaactg
    56101 tttggcgatt tgggtcctcg ttttactgct cgtaacggtg gttatgttcg ggtgttgaaa
    56161 tacggattcc gtaaaggtga taatgcacct ctggcactgg ttgaattggt tgacaaaccg
    56221 gctgctgagt aattttagtc atataacgcc atctgccgaa aagcaggtgg cgttattttt
    56281 gcaatatctg ataggtaata gggtattggc tatcatgttt aaaatattaa ttgaatagct
    56341 aaggtttgcg cggtaaactt acatcattaa aaaattctat gatggtttat ataatgaatg
    56401 ctttcgatat aaagtcgaca aagatggacg tattgtctat atctttgcat acgtcagact
    56461 tgtttgattt ggaagatgtg ctggtcaaat tgggcaagaa gtttcaagag tctggtgttg
    56521 ttccatttgt gctggatgtt caagagtttg attatcccga gtctttggat cttgctgcat
    56581 tggtttcgtt gttttcaagg catggtatgc aaattttggg tctgaagcat tctaatgaac
    56641 gttgggctgc tgcggctatg aagtatcatt tgctgttttg tctgtctcat tcggaaaatg
    56701 ttaaagaact gggtcaggtt gaggtgcaga acacggagga tggtcagaaa gcaaggaaaa
    56761 cagtattgat tacatcccct gtccgtaccg gtcagcaggt ttatgccgaa gatggcgatt
    56821 tgattgttac gggggcggtc agccaggggg cggaattgat tgcagatggc aatatgcata
    56881 tttatgcgcc gatgagaggg cgtgctttgg ccggtgcaaa aggcgatact tctgcccgca
    56941 tatttatcca ctccatgcag gcagaactgg tttctgtggc gggtatttac cgtaattttg
    57001 aacaggattt gccggaccat ctgcacaagc agccggtaca gatattgttg caggataacc
    57061 gattggttat cagtgcaatt ggctcagaat aattgtttga tattttaaaa aggaaatatt
    57121 gtggcaaaaa ttattgtagt aacttcaggt aagggcggtg tcggtaaaac gactaccagt
    57181 gccagtattg cgacaggttt ggcattacgc ggatataaaa ctgcggtaat tgattttgat
    57241 gtgggtttgc gtaacctcga cctcattatg ggttgcgagc gtcgtgtcgt ttatgacctg
    57301 atcaatgtca ttcaggggga ggcgacgctc aaccaggctt tgattaaaga taaaaattgt
    57361 gaaaacctgt ttattttgcc ggcttcccag actcgggata aagacgcttt gacacgcgag
    57421 ggcgtagaaa aagtgatgca ggagctgtcc ggcaagaaaa tgggctttga gtatattatt
    57481 tgcgactctc ctgccggtat tgagcagggt gcattgatgg cgttgtattt tgctgatgaa
    57541 gccattgtaa cgaccaatcc tgaggtttcc agtgtgcgtg actccgacag gattttggga
    57601 attttgcaaa gcaaatccca taaggcagag caaggcggtt cggttaaaga acatctgttg
    57661 attacgcgtt attctcccga acgtgtggca aaaggcgaaa tgctgtctgt acaggatatt
    57721 tgcgatattc tgcatattcc tttgctgggt gtgattcctg aatcccaaaa cgtcttgcag
    57781 gcatccaatt ccggagaacc ggtcatccat caggacagcg tggcggcttc cgaggcatat
    57841 aaggacgtta ttgcccgtct tttgggcgag aaccgtgaaa tgcgtttctt ggaagctgag
    57901 aaaaaaagct tcttcaaacg tctgtttgga ggataaggta tgtcattaat cgaattttta
    57961 ttcggcagaa agcagaaaac ggcaaccgtt gcccgcgacc gccttcaaat catcattgcc
    58021 caagagcgcg cccaagaagg tcaggctccg gattacctgc cgactttacg taaagagttg
    58081 atggaagtcc tgtccaaata tgtgaatgtt tcattagaca atatccgtat ttcccaggaa
    58141 aaacaggatg gtatggatgt gcttgagttg aacattactt tgccggaaca gaaaaaggta
    58201 taggacatga ccttaaccga attgcggtac atcgtcgcag tcgcccaaga acgtcatttc
    58261 ggcagggcgg cgcggcgttg ttttgtcagc cagcccactt tgtctattgc cattaagaaa
    58321 ttggaagaag agcttgccgt ctctttgttt gaccggagca gcaacgatat tattacgacc
    58381 gaggcggggg aacgtatcgt tgcacaggcg cgtaaggtgt tggaagaggc agagcttatc
    58441 aggcatttgg caaatgaaga acaaaacgag ctggagggtg cgttcaaact cgggctgatt
    58501 tttacggttg cgccgtacct gctgccgaaa ctgattgtct cgttgcgccg tactgcaccg
    58561 aaaatgcctt tgatgttgga agagaattac acgcatactt tgaccgagtc gctcaaacgc
    58621 ggggacgttg acgcgattat cgttgccgaa ccgtttcaag agccgggcat tgttaccgaa
    58681 cccttgtatg acgaaccgtt tttcgtgatt gtcccgaaag ggcattcatt tgaggaactg
    58741 gatgccgttt cgccccagat gctgggtgag gagcaggttt tgctgctgac ggaaggcaac
    58801 tgtatgcggg atcaggtact ctcaagctgt tccgaattgg cggcgaaaca acgcatacag
    58861 gggttgacca atacattgca gggcagctcg atcaatacaa tccgccatat ggtcgccagc
    58921 ggtttggcaa tcagcgtgtt accggcaacc gcgctgaccg aaaacgatca tatgctgttc
    58981 agcattattc cgtttgaagg tacgccgcca agccggcggg tcgtattggc gtaccgccgc
    59041 aattttgtcc gtccgaaggc gttgtcggcg atgaaggcgg cgattatgca gtcgcagctt
    59101 cacggggtaa gttttatccg cgactaggcg caggcattgt tttcaaaacg ccatttccct
    59161 gagccgacaa cacggtatgc caagatattg ccgtcatcat cgattttgag tatagcatcg
    59221 ccacggaaac tgccgtcctg aagatattcg acttttgcat cactgtgaat gttttcatca
    59281 gtgccgatgc aatgccatgt atagtggatt aacaaaaacc agtacggcgt tgcctcacct
    59341 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
    59401 tataaaagag gccgagacct ttgcaaaatt ccccaaaatc ccctaaattc ccaccaagac
    59461 atttagggga tttctcatga gcaccttctt cca
//
LOCUS       DAXHKX010000003        40495 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHKX010000003 DAXHKX010000000
VERSION     DAXHKX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.003"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..308)
                     /locus_tag="ACFMWZ_000162"
     CDS             complement(<1..308)
                     /locus_tag="ACFMWZ_000162"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC5934189.1"
                     /translation="MTIKSGGIADGYKGRLKDVVWKTLGEVFDLKNGYTPSKSNKEYW
                     ENGSIPWFRMEDIRENGRILDNSLKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIK
                     V"
     gene            complement(280..876)
                     /locus_tag="ACFMWZ_000163"
     CDS             complement(280..876)
                     /locus_tag="ACFMWZ_000163"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC5934190.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGDS"
     gene            complement(1042..2052)
                     /locus_tag="ACFMWZ_000164"
     CDS             complement(1042..2052)
                     /locus_tag="ACFMWZ_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HFC5934191.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(2049..3593)
                     /locus_tag="ACFMWZ_000165"
     CDS             complement(2049..3593)
                     /locus_tag="ACFMWZ_000165"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC5934192.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDYHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     YIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(3666..4670)
                     /gene="rfaD"
                     /locus_tag="ACFMWZ_000166"
     CDS             complement(3666..4670)
                     /gene="rfaD"
                     /locus_tag="ACFMWZ_000166"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC5934193.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LNWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVTFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKAGVERYVKWMLENLA
                     "
     gene            complement(4711..5829)
                     /locus_tag="ACFMWZ_000167"
     CDS             complement(4711..5829)
                     /locus_tag="ACFMWZ_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC5934194.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEVLLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(5831..7066)
                     /locus_tag="ACFMWZ_000168"
     CDS             complement(5831..7066)
                     /locus_tag="ACFMWZ_000168"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC5934195.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFSRVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(7103..8074)
                     /gene="hldA"
                     /locus_tag="ACFMWZ_000169"
     CDS             complement(7103..8074)
                     /gene="hldA"
                     /locus_tag="ACFMWZ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC5934196.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(8129..8869)
                     /gene="pyrF"
                     /locus_tag="ACFMWZ_000170"
     CDS             complement(8129..8869)
                     /gene="pyrF"
                     /locus_tag="ACFMWZ_000170"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC5934197.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(9395..10042)
                     /gene="adk"
                     /locus_tag="ACFMWZ_000171"
     CDS             complement(9395..10042)
                     /gene="adk"
                     /locus_tag="ACFMWZ_000171"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC5934198.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            10257..11096
                     /gene="htpX"
                     /locus_tag="ACFMWZ_000172"
     CDS             10257..11096
                     /gene="htpX"
                     /locus_tag="ACFMWZ_000172"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC5934199.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FSGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(11397..11957)
                     /locus_tag="ACFMWZ_000173"
     CDS             complement(11397..11957)
                     /locus_tag="ACFMWZ_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934200.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(12071..12268)
                     /locus_tag="ACFMWZ_000174"
     CDS             complement(12071..12268)
                     /locus_tag="ACFMWZ_000174"
                     /inference="COORDINATES: protein motif:HMM:NF019096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HFC5934201.1"
                     /translation="MKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALNTPYGSIR
                     TKLDGRSNPDNLVCNRYKVLL"
     gene            complement(12290..12463)
                     /locus_tag="ACFMWZ_000175"
                     /pseudo
     CDS             complement(12290..12463)
                     /locus_tag="ACFMWZ_000175"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            complement(12671..13063)
                     /locus_tag="ACFMWZ_000176"
     CDS             complement(12671..13063)
                     /locus_tag="ACFMWZ_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC5934202.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(13180..13590)
                     /locus_tag="ACFMWZ_000177"
     CDS             complement(13180..13590)
                     /locus_tag="ACFMWZ_000177"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC5934203.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(13604..13987)
                     /locus_tag="ACFMWZ_000178"
     CDS             complement(13604..13987)
                     /locus_tag="ACFMWZ_000178"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC5934204.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(14453..15751)
                     /locus_tag="ACFMWZ_000179"
     CDS             complement(14453..15751)
                     /locus_tag="ACFMWZ_000179"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC5934205.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRVADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGTLLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(15855..17006)
                     /locus_tag="ACFMWZ_000180"
     CDS             complement(15855..17006)
                     /locus_tag="ACFMWZ_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC5934206.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVVDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            17341..18720
                     /gene="norM"
                     /locus_tag="ACFMWZ_000181"
     CDS             17341..18720
                     /gene="norM"
                     /locus_tag="ACFMWZ_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC5934207.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSSLALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLELCSR
                     EMVRSHKVV"
     gene            18764..19804
                     /gene="murB"
                     /locus_tag="ACFMWZ_000182"
     CDS             18764..19804
                     /gene="murB"
                     /locus_tag="ACFMWZ_000182"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC5934208.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNIVLMQDYAGLVVHMENKGIREIARSDGIVRIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHGRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            19816..20466
                     /locus_tag="ACFMWZ_000183"
     CDS             19816..20466
                     /locus_tag="ACFMWZ_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC5934209.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            20521..21297
                     /locus_tag="ACFMWZ_000184"
     CDS             20521..21297
                     /locus_tag="ACFMWZ_000184"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC5934210.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFFTEEAAKLDLPAPDFAPDDSRGKIIRTVCDNGLSL"
     gene            21348..21914
                     /locus_tag="ACFMWZ_000185"
     CDS             21348..21914
                     /locus_tag="ACFMWZ_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC5934211.1"
                     /translation="MSALLPIINRLILQSPDSRSELATFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            21934..22824
                     /locus_tag="ACFMWZ_000186"
     CDS             21934..22824
                     /locus_tag="ACFMWZ_000186"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC5934212.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            22849..23607
                     /locus_tag="ACFMWZ_000187"
     CDS             22849..23607
                     /locus_tag="ACFMWZ_000187"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC5934213.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVLPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEDDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(23662..24327)
                     /locus_tag="ACFMWZ_000188"
     CDS             complement(23662..24327)
                     /locus_tag="ACFMWZ_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC5934214.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDEARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYAEMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(24525..25298)
                     /gene="folE2"
                     /locus_tag="ACFMWZ_000189"
     CDS             complement(24525..25298)
                     /gene="folE2"
                     /locus_tag="ACFMWZ_000189"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC5934215.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            25490..26647
                     /locus_tag="ACFMWZ_000190"
     CDS             25490..26647
                     /locus_tag="ACFMWZ_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC5934216.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQQGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGVVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFY
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(26710..27711)
                     /gene="hemB"
                     /locus_tag="ACFMWZ_000191"
     CDS             complement(26710..27711)
                     /gene="hemB"
                     /locus_tag="ACFMWZ_000191"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC5934217.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(27894..28178)
                     /gene="yhbY"
                     /locus_tag="ACFMWZ_000192"
     CDS             complement(27894..28178)
                     /gene="yhbY"
                     /locus_tag="ACFMWZ_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC5934218.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            28288..28908
                     /locus_tag="ACFMWZ_000193"
     CDS             28288..28908
                     /locus_tag="ACFMWZ_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC5934219.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            28972..30939
                     /gene="ftsH"
                     /locus_tag="ACFMWZ_000194"
     CDS             28972..30939
                     /gene="ftsH"
                     /locus_tag="ACFMWZ_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC5934220.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            31160..31591
                     /locus_tag="ACFMWZ_000195"
     CDS             31160..31591
                     /locus_tag="ACFMWZ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC5934221.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            31584..31862
                     /locus_tag="ACFMWZ_000196"
     CDS             31584..31862
                     /locus_tag="ACFMWZ_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC5934222.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            31915..32493
                     /gene="pth"
                     /locus_tag="ACFMWZ_000197"
     CDS             31915..32493
                     /gene="pth"
                     /locus_tag="ACFMWZ_000197"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC5934223.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            32554..32760
                     /locus_tag="ACFMWZ_000198"
     CDS             32554..32760
                     /locus_tag="ACFMWZ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC5934224.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            32757..33119
                     /locus_tag="ACFMWZ_000199"
     CDS             32757..33119
                     /locus_tag="ACFMWZ_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC5934225.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            33350..34765
                     /locus_tag="ACFMWZ_000200"
     CDS             33350..34765
                     /locus_tag="ACFMWZ_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC5934226.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            34884..35393
                     /locus_tag="ACFMWZ_000201"
     CDS             34884..35393
                     /locus_tag="ACFMWZ_000201"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC5934227.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTIVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            35560..36942
                     /locus_tag="ACFMWZ_000202"
     CDS             35560..36942
                     /locus_tag="ACFMWZ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC5934228.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPK
                     NLQDLIVELKNGEAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKAHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIDLPEGSNGHKVIEELAAN
                     AKFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(36962..37717)
                     /locus_tag="ACFMWZ_000203"
     CDS             complement(36962..37717)
                     /locus_tag="ACFMWZ_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC5934229.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPQDLFDHPKHERTRRFLSQIQSTKI"
     gene            complement(37727..38443)
                     /locus_tag="ACFMWZ_000204"
     CDS             complement(37727..38443)
                     /locus_tag="ACFMWZ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC5934230.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIARKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(38433..39260)
                     /locus_tag="ACFMWZ_000205"
     CDS             complement(38433..39260)
                     /locus_tag="ACFMWZ_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC5934231.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            39545..40453
                     /locus_tag="ACFMWZ_000206"
     CDS             39545..40453
                     /locus_tag="ACFMWZ_000206"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC5934232.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKSACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         9741 a        10987 c         9926 g         9841 t
ORIGIN      
        1 acttttatta acgcatgctc tcctatggtt gccgttgtcg acatcattat tgatttagca
       61 ggaaacagct tgccgccctt aactgcgctt tttgatatat gcttcaatga attatctaaa
      121 atacggccat tttccctaat atcttccatt ctaaaccaag ggattgagcc attttcccaa
      181 tattctttat ttgatttgga aggggtatag ccattcttca agtcaaacac ctcccccaac
      241 gtcttccaaa ccacatcttt cagacggcct ttatagccat cagctatccc ccccgatttg
      301 attgtcaaaa tctaaaagaa agtcgcggta ataccggtat tggcgtttgc gcagggctaa
      361 ttcagcttcc aactcggtga atttgtcaag tatttttaca attttttgtt gggtttccag
      421 gggtgggata gggattaata attttttcaa gttactggca tttagagcag gaatacctgt
      481 aaatgtacat aaggcatgaa tctcttgttc aaattcaagt aatatatgat agagaaaacg
      541 aacatccaat tctgttctat tcttaatagt aactgcataa tttaagttgc cacgaaaata
      601 tcttccttct tgccacgtta ttgagccgac tcccgctcct cttgttgtga tgccgatagg
      661 gtcattttca gtgttccatt catcaatata gccaagcggc tctttgccac tgttaataac
      721 tggataacta ccaatattat ttgagatttt ctgcttactt acagcttgtc ctgtttttat
      781 gatagctatc ccattttctc cacccaacgg cttccactcc accggtgccg tctgaatcat
      841 ctcaatcaat tttttcgctt tgctttgcat atccatggtt gttttccgtt tctttcccgc
      901 tgtggcggga tgtggggcgg ttgtggggtg cttcggctca ctctgtccgt ttgccgttat
      961 ttctgcgggc tgaagtccgt cctgctttca ggctgcctgc aatggtgtgc aggctgcttt
     1021 ttcaggtagc ctgaatggta gttattttgt gcggtctttt ttctgtttga tctgttgcac
     1081 ggctgttttt tccaacgctt taacgctttc cagataggct tgctccaccg gcgacaggat
     1141 gcgcgcttca taggcgcggt attcgcgttc ggctttctgc tctgcctgtt tgcggctgat
     1201 gctgcctgca ccctgtaatg tgccttgtcc gtacagcccg gaaaatttgt ccaattcggc
     1261 tatccagtcg cgcatataca tggggctttg ctcctgcgct ttgatttccg ccaggtcgaa
     1321 gaaggcggaa accagacggt tcaggcggaa cagttcgtct tcggtcagat agtttttggc
     1381 gattttggct tcattcagcg tggggattgc gccttgaaag gtggtcagcc ccataaagtc
     1441 tttgctgctg tcggcacggc tgtatatcag ctcagctgcg gtttgccggc tggcggcata
     1501 gtgcagtttg ttttgaacgg cggcaaaaaa ggtttggctt tcgctgcttt tggggttgta
     1561 gtcttggctg gtggcatata aatcaagcac ttgccggtat agggcttttt cgctgctgcg
     1621 gatgtcgcga atgcggttga gcagttcttt ccaatagtcg ccgccgcctg tgcctttcag
     1681 gcgttcgtcg tctatggcaa agcctttggt cagatattcg tccagccgtt cggttgccca
     1741 ttggcggaat tggatgcccc gcgcggaacg gacgcggtag ccgacggcaa taatcatggg
     1801 cagggaataa tgggcgattt tgcggtttac cttgcgcccg ttttcatttt gaactgtcaa
     1861 ctggaagttg acagttgcct tctcttccaa ttcttgctct gcaagaatgg ttttaatgtg
     1921 tttgctgata ttttgtttgg tggtttggta cagttctgcc atgtccgcct gcgtcagcca
     1981 aagctgtccg ccaaattcct gcaaggcaaa ttgggcagtg ccgtcgttgg cggtgtatag
     2041 gatgatgctc atgcttcaat ctctgcaatc acttcgtcaa tttcacgccg cagccgttcg
     2101 attttggcaa cggtttcgct gatttcggcg ttaagctgtt tgatgtcgat aatttcgcgg
     2161 gtgtcttcgg gttcaacata gctgctgacg gcgaggttgt agccgttgtc tttgacggtt
     2221 tgctgggcgg cgttttgggc gatatgcggc acatcggctt tatcggcgaa gagtttgacg
     2281 atttcggcaa tgtgttcttc ggttaagacg ttgttgttgg tttctttttt aaagaagccg
     2341 cctgcgtcga tgaattggat gtcggtattg tctttgtgtt tggacaaaac cagtatattg
     2401 acggcgatgc cggtgccgta aaagagattg ggcgcaaggg cgatgacggt ttccacgtag
     2461 ttgccctcca ccagatattg gcggattttc tgctctgcgc cgccgcgata gaaaatgccg
     2521 gggaatgaga cgatggcggc gcggcctctg ccggaaaggt agttcagtgc gtgcagaatg
     2581 aaggcaaaat cggctttgga tttcggggcg agtacgcctg caggggcaaa gcggtcgtcg
     2641 ttgattaagg tggggtcgcc gctgcctatc cagttgatgg aatagggcgg attggaaacg
     2701 acggcatcaa agggtttgct gtctttgagt ttgggattgg tcagtgtgtc gcccaattcg
     2761 atgtagaatt tgttgtaatt gacgttgtgc agaaacatat tcatgcgggc gaggttgtag
     2821 gtggtgtggt tgatttcctg cccgaagaag ccttcttcga tgatgtgctc gtcaaactgt
     2881 tttttcgcct gcaagagcag gctgcccgag ccgcaagctg ggtcgtagat tttgtttact
     2941 ttctcctgcc cgtgcaccgc cagccgcgca atcagcttgg atacgctttg cggggtgaaa
     3001 aattcgccgc cggatttgcc tgcgttggca gcgtagttgg aaatcaggta ttcgtaggca
     3061 tcgccgaaaa ggtcgatgtg gtagtcttca aaactgccga aatcgagttc cgccacgcct
     3121 tttaggacgg cggcaaggcg tttgttcttg tcggcaacgg tgctgccgag ccggctgctg
     3181 gtggtgtcga agtcgtcaaa caggccttta atgtcttgtt cggacggata gccggaggcg
     3241 gagctttcaa tcgcggtaaa aatttctttc agcttggtgt tgagctcttc gttttgatgg
     3301 gcttcggcgg caatattgca aaaaagctgg ccggggtaga tgaaatagcc tttgactttg
     3361 acggcatcgt ctttgatttc gggcgtgatg atgctgtccg gcatagcggc gtaatcaata
     3421 ctgctgtcgc ctgcctgcat atagtcggta aagttttcgc tgataaagcg gtagaaaagt
     3481 gtgccgagaa cgtattgttt aaagtcccag ccgtccaccg cgccgcgtac ttcgtcggca
     3541 attttccaaa tttggcggtg cagttgggcg cgttgttgca tttcggtcat catcgaaatc
     3601 catataaaag ttaaacaaat cgaaatcgcc tgatattttc agacgatttt ttacgggcat
     3661 tcaaattaag ccaaattttc cagcatccat ttgacgtagc gctcgacgcc tgctttgaca
     3721 tcgaaaaatt cttccgtata acctgcctca cgcaatttgg tgatgtcggc ttgggtgaag
     3781 ctctggtatt tgcctttgag tgcgtcgggg aaggggatgt agcggataag ttcttcttcc
     3841 accagctctt tcaagctcat ttcaggcttg ccttcggcgg cgcggcaggc gttgacggtg
     3901 gcggcggcga gttcgttaaa ctgttggctg cggccggtac cgaggttgta gatgccggaa
     3961 agttcgggat ggtcgaagaa gtagaggttg actttggcaa cgtcttcgac gctgacaaag
     4021 tcgcgggttt gttcgccgtt gccgtaaccg tcattgctgc cgaacaggtt gacgtaaccg
     4081 tgttcgcggt attggtggaa atggtggaag gtgacggatg ccatgcggcc tttgtgttgt
     4141 tcgtgttgtc cgtaaacgtt gaagtagcgg aagccgacga cttgggcggt gagaccttct
     4201 ttcatgcggc ggcgcaatac ttggtcgaac aggaatttgg agtagccgta cacattaagc
     4261 ggtttttcga gttcgcgctc ttcgcggaag atttcgcctt tgccgtaaac cgccgcactg
     4321 gaggcataaa ggaagggaat gcgttcgtcc tgacaccagt tcaacaaatc cagcgtgtac
     4381 tggtagttgt tttccatcat atacaaaccg tcgtggttca tcgtatcgga acacgcgcct
     4441 tgatggaaaa cggcttcgat gttttgataa ggtaaaatgt gttccctcac ttggcggatg
     4501 aattcgtgtt tgtcgagata atgggcgatt tcgcactcgg caaggttttt gaatttttcg
     4561 cctttgctca aattatcgac ggcgacgatg tcggtaatac cgcgttgatt aagtgctttg
     4621 acgatgttgc tgccgataaa gccggccgcg cctgttacga tgatggtcat attcggtttc
     4681 ctttgttttg agtaaaatta ataaacttag ttacaagaaa tcatcgatat tgtcctgaaa
     4741 cacaaatttg cgatgatatt ccaaatattt ttctcttcct tcgggatata ttctcatctt
     4801 attttttgca tttggattga tattaagttt ttcccatgtg taacggctta gtcgagtacc
     4861 gcaaatcaaa cgaccatcat ccaagaaagt aatataacct tgatcgaaca accaatcata
     4921 agtcggcgtt aaagctaacc catttaaata atctaatgcc tctttctcat ttttctcagt
     4981 aatacaaacc atataaggtt taatatgact agcccttaac aaaacctcat ctgtaattaa
     5041 tgtaaatgga cattgcggca tatgatttat aacatccttg cgatatttct gctgcccttt
     5101 tctacttttc tgtttaatga tttcttgctc tacgaattga ttaaattctg aagccggtat
     5161 ttctaatttt tctcttgata agagttgcgg gtgcacgata gagcgaaatt gataatccaa
     5221 aaatattcgg aaataaaata atggttctga atcttctata tctttgacag gcaatagttt
     5281 aagaattgat aaataactaa ttttgggcaa tacaatctta cgccaaattg accatatatc
     5341 atcctccgaa cggatataac ccctacggta acctttagaa ttttccaaat ggtctgatac
     5401 atcatggatt gaaaagaaaa tattttctgt aggcagatta ttaatactta aaacgtaatc
     5461 atcataaaac cttgaaatat catgatgata tttctgttct tgtttgtgat attcaacttt
     5521 tgcatcaacc aaataacgct gtaaattttt cttattaaaa aaacaatttt tctgaatgag
     5581 aggatattct accctccttc cattttttgt ctcagtaatc ccccaatttt catactcaaa
     5641 aaatgtactt agttttcgac cactatcatc aatataacta cctacatatt tgcgtgcttc
     5701 cccattacca ccaaaaacag ataacttgtt attcctgtga ataaaactat cttccgcacg
     5761 taaattttga atactatcga caataaaata ctctatgttt tcaatagtaa ttctttgaaa
     5821 atccggcatt ttactctcca taaagcgtaa tatccatttg ttttaatata gcgattaata
     5881 catcaacaac aatactgttt cccgcttggc gatacatttg agtatcacta actaaaattt
     5941 taaaatcatc tctaaaaccc attagccgca aacattccct aggtgttaat ttacgaatac
     6001 gtccacgatt atgagtaaca taattatcaa ccccggcacg atgcatttta tgcatagttt
     6061 gcaataatgg gcgagctaca ggcaaatcag tttccgtact ggttttaaaa ttttttgttc
     6121 ctcctgccaa aacataattt ttgatttttt cagaaagata atatttttcc tcaacgttat
     6181 ttacatcaaa aataaagtca tcaaattcag attcgcgggc tgcctgaaaa ataaaatcac
     6241 catgccaatt aaattgttga tttgcttttt ggcataaggc aatttctcca ttaatctgtg
     6301 tataacgttt ttgtctattt ttagaactgg taacaaattt cacacccttt tcacgtaaaa
     6361 aatatttgct atcagtatag tcctccaaaa aatcttgcat agtatgttct aattcaatct
     6421 tttcaggaaa ctgaaaacca tttataggag gggtatgaaa gccaacaaca aaaatacgct
     6481 cacgatgttg aggaatccca taatccttac tattcattat ttggaaatat aagtcataac
     6541 caagtgaata aaaaacactt tttacaactt tccaagtttt tccattatca tgattgagca
     6601 agccctttac attttcataa ataaatatct ttggttggac ttcatccaca acgcgagaaa
     6661 attcatagaa taatgttcct cgtgtatctt ctaatcctgc acgtttgcca accatggaaa
     6721 atgcttggca aggactgcct ccaactaaaa tatcaacttg atttctaaac tttctcgcat
     6781 caaattgagt aatgtcgtta tgccaaaaat cttcatttaa tttatagttt ccaagataac
     6841 tttttttaac gtatggatca atatctcctg caaaaacaat ggtatggttt aaatttaatc
     6901 ggtggaatgc ctgttcaacc gcaccaatcc cgctgaatac ggttgctaat ctaatatgtg
     6961 aatcaggttt aagaaaagtt ttagatttcc aaccttgttg actgggaaag agcaaagttt
     7021 tttgtaatcg agtatcgtgt gtctgtgcca ttgtcgaaat agtcatactt atatcgttct
     7081 gtttatctta tcaatatgaa aactacattg ttgattgccc tgacaatgcc ttgatcaatt
     7141 cggcaaacga gcaaaccgcc gtaccgagtt tcgccacgac aaccccggcc gcagtattgg
     7201 caaggtacat ggcttcgggc atggtgcagc ctgccgccaa acccaagccc attccggcaa
     7261 tgacggtgtc gcccgcaccg gatacgtcgt aaacttcttg ggcgcgggtg ggctggtaaa
     7321 tcggttcgcc ttcgctgaac aaggtcatgc cttcttcgct tcgggtcagt aaaacggcgg
     7381 tcaggtcgag gtggcggcgc aggttttgcg ctttttcggt cagctcgctt tcgtttttcc
     7441 aactgccgac cacttctttc aattcggcgc ggttaggcgt aatcagagtt gcaccgacat
     7501 atttttcgta atcgtcgcct ttggggtcga ttaagacggt tttgccggcg tgtttcgccc
     7561 aatcgatcat atcggagata tgcgacaggc cgcctttgcc gtagtctgaa aaaatgattg
     7621 cgtcgtattc gggcaagatt tcgcggtatt tctgcttgat ttgttccaac acttcgcagt
     7681 tgggatgttc ttcaaaatca agacggataa gctgctggtt gcgggcgacg acgcgcagtt
     7741 tgacggtggt ggcgatttgt ttgtcgcgca tcagatagga ggcgacgccg tcctgcacca
     7801 tcagcgcatc gagcgcgtcg gcggcttcgt cgttgccggt tacggacaac agccctgccc
     7861 tgccgcccaa cgaagcgatg ttgcgcgcga catttgccgc tccgcccgcg cgttggtcga
     7921 ttcgtccgat tttcgccacc ggcacggggg cttcgggcga aatacgggac acatcgccga
     7981 accaatagcg gtcgagcatc acgtcgccga caaccaggac tttggcttgc gcgaaacggg
     8041 atttgagggt ttcttgttgg aacttggcgg acatattgcc tccgttgtat tttttcagac
     8101 ggcctcagcc tgtaaggccg tctgaaaatc agtttgcttc aaggtttgcc acgcggttca
     8161 cttcgcgcaa tacggctacc ggatcggcag cttgggttac aggacgaccc attaccaaat
     8221 aagtcgaagc ggcagccaag gcttcggccg gtgtcatgat gcggcgctgg tcatcattat
     8281 tgccggcaac gtccaagcgg atgccgggcg tgaccaagac aaaatcttgt cccaactcgc
     8341 ggcgcagcgg tgcggcttct tgggcggaac agaccacgcc gtccaagccc gaactttgcg
     8401 ccagtttcgc caagcggatg acttgttctt caggggcggt gttcaaaccg atttccgcca
     8461 aatcgctttg ttccatgctg gtcaacacgg ttacgccgat taagagcggc ttcgtgccgt
     8521 atccggcaac ggcttctgct gcggcttcca tcatacggcg gccgcccgat gcgtgcatat
     8581 cgaccatcca aacgcccata tcggcagcga ctttgcaggc ttgcgcgacg gtgtggggaa
     8641 tatcgtggta tttcaaatcg aggaaaagtt tgaaaccttg attgattaag ctttctgcca
     8701 aattgcgtcc cgtcgcggta aacagctctt tgccgatttt gatttgacac aatgtcgggt
     8761 caaggttgcg gacgaatccg agcgtgtctt tttcgttgga aaaatcaagg gcgacgataa
     8821 cgggggtgcg ttgttgcgga gtttggaagt cggagattaa aggattcatg gtgttttcgt
     8881 ttcgggtttt cagacgacct ttatcgtctt tttcgcagta tagtggatta actttaaacc
     8941 ggtacggcgt tgtctcgcct tagttcaaag agaacgcttc tctaaggtgc tgaagcacaa
     9001 agtgaatcgg ttccgtactg tttgtactgc ctgcggcttc gtcgccttgt cctgattttt
     9061 gttaatccac tataagaacg taaattcaaa ccgtaggtcg ggcattgatg tccgacctac
     9121 gcggactcaa gcctccgaca aactgtttcg gataaatttg aaccggtaca tcagccgtca
     9181 ttcccgcgta ggcgggaatc cattgataaa actcagttca tcagatttaa acagcgattg
     9241 cccaaattca gaaatggatt cccgcctgcg cgggaatgac gccaaatgat tatttttcgg
     9301 ttgcaacacg ctgtttatca aaaacaggtc gtctgaaaac ctgattttct gttttcagag
     9361 tgcgaagcct gcccgtgggt acgacctttt cgatttattt gcccaatgcg cccaatactt
     9421 cggctttcac ggcttctacc ggctgagtgc cgtcaacttt gatgtattta ggcgcgtgtt
     9481 cgccttccag tttgctgtaa aaatcgacca aaacttcggt ttgctcgtgg taaacggcaa
     9541 ggcgtttttt cacggtttct tctttgtcgt cgtcgcgctg aatcaaatct tcgccggtta
     9601 cgtcgtcttt gccttcaact ttgggcgggt tgtaggtaac gtggtaagta cggccggaag
     9661 ccaaatgcac gcggcggccg ctcatgcggt cgacaatcac gctgtcaggc acatcgattt
     9721 caacgactgc atccaaatcc acgcctgctt caaccatcgc ttcggcttgt gccaatgtgc
     9781 gcgggaaacc gtcaaacagg aaaccgtttt tgcagtcgtc ttgcgcgatg cgttctttga
     9841 ccatgccgat aatgatgtcg tcgcgcacca agccgccttc gtcaatgatt tttttcgctt
     9901 ccaaacccaa gggcgtgcct gccttaatcg cggcacggag catgtcgccg gtagagattt
     9961 gcggaatgcc gaacgctgcg gtgatgaatt gcgcctgagt gcctttgccc gcgcccggtg
    10021 cgcctaaaag taatgctttc atgaagtgat ccttttaaat gtgtttattg gaatcaaaca
    10081 gatggtttac gattctacac ggcttggcac aatagttcaa aaaccaactg cacaaactgc
    10141 catttgcaag caaatgtttt gcataccttt gccgagcctg atattcacaa ggcggcaact
    10201 ttttgggaca atccccaaaa tttcaaaaca tcacacccta ccaaaaagga acatccgtga
    10261 aacgcatttt tctgtttttg gctaccaata tcgctgtttt ggtcgtaatc aacattgttt
    10321 tggcggttct aggcatcaac agccggggcg gcgcgggcag cctgttggcg tattccgccg
    10381 tcgtcggctt cagcggctcg attatttcgc tgctgatgtc caaattcatc gccaaacaat
    10441 cggtcggtgc ggaagtcatc gacacgccgc gcaccgaaga agaagcctgg cttttgaaca
    10501 ctgtcgaagc ccaagcgcgg caatggaacc tgaaaacgcc cgaagtcgcc atctaccact
    10561 cccccgaacc caatgccttt gccacgggcg catcgagaaa cagctccctg atcgccgtca
    10621 gcaccggttt gctcgaccat atgacgcgcg atgaggtgga agcggtgttg gcgcacgaaa
    10681 tggcacacgt cggcaacggc gatatggtta cgctgacgct gattcaaggc gtggtcaata
    10741 cctttgtcgt attcctgtcg cgcattattg ccaacctgat tgcccgaaac aacgacggca
    10801 gccagtcgca gggaacttat ttcctagtca gcatggtatt ccaaatcctg ttcggcttcc
    10861 ttgccagctt aattgtcatg tggttcagcc gacaacgcga ataccgcgcc gacgcgggcg
    10921 cggcaaaact ggtcggcgcg ccgaaaatga tttccgccct gcaaaggctt aaaggcaacc
    10981 cggtcgattt gcccgaagaa atgaacgcaa tgggcatcgc cggagatacg cgcgactccc
    11041 tgctcagcac ccacccttcg ctggacaacc gaatcgcccg cctcaaatcg ctttaaaccg
    11101 atttgaaacg gcaaaaaccg tacgcgcaag cagtacggtt ttttgtatat gctgtctgaa
    11161 aacatcaggt ttccataccg gcaccaaccg tatccatttc cgcaccggat acgccgcatt
    11221 gatataccct gataaaccgt atccggacag aaaatatagt ggattaacaa aaaccagtac
    11281 ggcgttgcct cgccttagct caaagaaaac gattctctaa ggtgctgaag caccaagtga
    11341 atcggttccg tactatttat actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
    11401 tcccctatat cagataaaac agcggtaaaa ttgccgtctg aaaccaactc tgcgccatac
    11461 gggacacgct ctgcacgtct atttgcctcc gatatatcct ccctactgta ggaacgtctg
    11521 ccgatttgca cgaaatcggc acctctagca cccttcagga tggcggacgt tatccgttct
    11581 tgggattctg caagacggtc gacggcatta tacagacgct cggtctgata cccttccgaa
    11641 gcaacctgct tcaggtattc caaataaccg taacccaacg cccctccgac aagcagggtc
    11701 gtcaacgcta ccagcgttgc cgaacggatt ttagggggca tatcttgaag catatctcta
    11761 ccgaaccgaa ccaaagtctc tttaaagtcg gactctacac gcgtagaacc ctcggaaatc
    11821 ttgaagttca actcatactg cttccgtttg tccgctgtca accgcttgag gttgtcatcc
    11881 ccgtattgga cggttgacac aaccctgtac agactgtcct gtatttcgac cagcgtctgt
    11941 gcaaagttgg cggtcataac tggattccca acccttgccg ccgattctga tactcaaaac
    12001 ggtatccagg cgttccagca gggcttcggg agacagcagt gcgtattctt caatattttc
    12061 cataggttga ttatagcaga accttatacc tgttacagac aaggttgtcg ggatttgaac
    12121 gtccgtcgag cttggttcgt atagagccgt agggggtgtt tagggctttg gcggcagctt
    12181 gtacgctggg gaacgtgagt gtttcgcctg tggcggtgtc gaggatggag acaggttttt
    12241 ggttttgggg aggcgtattt tccttcattt taagtttccc gcactccgga cacccatact
    12301 ttgactttat agctgtgtcg taatacaaaa gttggacaac cccgtgcgta ggacatctaa
    12361 cactggaagg tttataaatg ccactgaact ccaacaattc taaatgtgga aatttctctt
    12421 gaaagcgtga tttggcggta tcgaaagtca ttcgcttacc cataatttgt ctccataggt
    12481 tatgtgcaaa tggattacac aaccaaaata tagaaatttt caaatttttt gagacctacc
    12541 tgaaatcctg cgcaagcaca atgtaaagaa aaatggtata cgtctttact aaatctttac
    12601 ataaattaac tctaaccggg atttgaacgt ccgtcgagct tggttcgtag gatttagggt
    12661 gttgtgaacc ttacctatcg tacccaagca ctttgtacct gtttgaaata aggttgtcgg
    12721 gcgacgttcg acccttgagc ctgtgattga tttgttggaa atgtacacct aaagcagcac
    12781 ctgcggcagt aacgcttggg aacgtgagtg tttcgttggt cgttgtgtcg aggatgagga
    12841 ggggcttccc tacatttgga ggtgtttggg tttttgacat cttagcacac tcaggacacc
    12901 cgtactttga ctttataagg gcattaaatg tcgaataacg aacgttcccg tgcataggac
    12961 aattaacggt tactgggtaa ttcacaccgt caaagtcggt tagagagata ttagggaata
    13021 tatcagtaag ttcagaagct gcttggttga aagatttggc catttgagac tccaaaattg
    13081 attgtcaaat ggatttaata gcaaatttat aaaaatgtca aatttttcga gaagcacctg
    13141 atagtggcga ggggtataca ctgttaaaaa gtgtatacgt catttagtgc agatgtgaac
    13201 cttatacctc cctgcaagta aagtctctgt gtgaacccga ccttttatac ggtcagtgat
    13261 agctgctggg tttataccca atgaccttgc agcagatgct cttgagggga atgtaagtgt
    13321 ttcgccagtt acagtatcct ccaatactgt atttttagat aatttatcta ttgtatcttc
    13381 tggaattttg tgggaattga caccataaga tgcacatttt ggacacccac ttttagagtc
    13441 caacatactt ttgaatgtag agcaagtaac cctcccgtgt ataggacaga cgattaccgt
    13501 cgggtaacga acgccattga actccacaag attcaaatgt ggaaatttag aggttagctt
    13561 agaaactgct tgagtaaatg taagtgccat agtggttgtg aacttacttt accaacctat
    13621 aacgatacct aatcaatcgg tcaaacggag tgcgtccgct taaacgtaca gctaaagtgc
    13681 tgttggagca accgataaat ttactggcag ccagtgtact tgcaaactcg tagatttccc
    13741 ctgtctctgt gtcctccaac ctaatggcaa cggcgtttgg agagggcttt tcgggatttc
    13801 gagtacattc aggacatcct gttgaggatt tcatggcagc ttgtagattc ccgtaaacaa
    13861 ccgacccatg caaagggcat tggattgtta ccggttcgta gtttttagtg aacttcaaaa
    13921 ccgtcttatc aggaaattta ctggaaaacc ttaactgaac atcttgtagc gagagtgatt
    13981 ttggcattat agacctttct aaaatgaaag cattatacaa ccaaaatata gaaattttca
    14041 aattttctct gaacgcactg gtatccgatg gagctaaatg taaagaaaaa tggtatacgt
    14101 ctttactaaa tctttacaca ttttaactgt atagtgtttg acaaacaaca ggcataggcg
    14161 cataatgcac ccgtagttag atagatagta aggagataac caatgactta gcacctgccc
    14221 ccctctctct cgcgcacccc gcccctcgct tgcggctaat gggctttgcg cgacatatac
    14281 actttcatct cctgcatggc ttaactgcct atgcaatagt acacacatca acaaatcagg
    14341 ctggcacgtc ttggcattgg ctgacgtacc attgtagccc gaaatgttga taccctacta
    14401 tttatgacca acggcggatt agggcttcag acggcaattt tactgcaaaa ccttatgcga
    14461 acggatgatg cagcacaatc gtttcttcgc ggtcggggcc ggtggagacg atggcgaccg
    14521 gcgcgccgca gacttcttca atccgtttca aatatgcttt ggcgttttcg ggcaatgcgc
    14581 cgtagtcctt cacgccgaaa gtggattcac gccagccggg catggtttcg taaatcggct
    14641 tgcaggcttc taccgcatca gagccgcaag gcaggatgtc ggttttgccg ccgtcgggca
    14701 attcgtagcc gacgcagata ttgatggttt caacgccgtc cattacatcg agtttggtaa
    14761 tgcacatacc ggaaatgccg ttgatttgga tggagcgttt cagggcggcg gcatcaaacc
    14821 agccgcagcg gcgcgcacga ccggttacag aaccgaattc gtgtccgcgc tccgccaaac
    14881 ctgcgcctac ttcgtcgaac aattcggtcg ggaacgggcc cgaaccgacg cgcgtggtat
    14941 aggctttgac gatgcccaaa acataatcca gcatttgagg gcctacgccc gcgcctgccg
    15001 aagccgcgcc ggcgaggcag ttggacgagg taacgaaggg gtaagtgccg tagtcgatgt
    15061 ccaacagcgt gccttgcgcg ccttcaaaca gcagtttttc gccatttttg tttttctcgt
    15121 tcaacacgcg ggatacgtcg gtaatcatcg gcgcaatgcg cggcgcgact ttttcgataa
    15181 ccgccatcac gtcttccgct ttcaccggct cggcattgtg cagatgttgc agttggacgt
    15241 tgtaataagc gaggacggca tccagttttt cacgcagttt ttcaggatgc agcaaatcgg
    15301 cgacgcgaat ggcgcggcgt gccactttgt cttcgtaggc aggaccgatg ccgcggccgg
    15361 tcgtgccgat tttgcctttg ccgcgcgatg cttcgcgggc ttggtcgagc gcgatgtggt
    15421 aaggcaggat cagcgggcag gtcggcgcga ttttcagacg gccttcgacg tttttcacgc
    15481 ctgcagcgtt taactcgtcg atttcgccca acagggcttc gggggagacg acaacgcccg
    15541 aaccgatgaa gcagtccaga ctttcatgca ggatgccgct cggaatcagg cgcaaaatgg
    15601 tttttttgcc gccgacgacc aaagtatgac ccgcattgtg gccgccttgg aagcgcacca
    15661 cgccgccggc ttcttccgcc agccagtcaa cgattttacc tttaccctcg tcgccccact
    15721 gtgcgccgat tactacaaca tttttagcca tagccatata acctatcgat attaaaaatt
    15781 attgtgggaa tccccaaacc ggtgcggctt tgcagcctgc cacggtttgc ctttcatccg
    15841 ccatgaacgg gtatttagcg tttcacgacc tgccaaacgc cgtccgtctt tttcagacgg
    15901 cctgcaagct cttccgaaac attgtgtccg ataccgtagt caaccacgac acactgccct
    15961 tgttcacgca aggcttcgac cgcttcgcgc gccgcttcgg catcttccgc atcgaccaac
    16021 acggcgggct gccgttcgat ggcgggcaaa cgcccgataa agctgcgcaa gtcgaaactg
    16081 aatcccgttg ccgggcgcgc cctgccgaaa tatccgccca atccgtcata acgcccgccg
    16141 cgcgcgaccg cgtcgtggaa atcggcggca taggcggcat acagcaagcc cgtgtggtaa
    16201 ttgtcgacac gcagctcgga caagtcgata tggatttcac aatcggggaa tgcgtcgcac
    16261 accgcctgca attcgtctaa cgcgccgccg accgccgaca aatccggcaa ccgtccgcgc
    16321 gcgtcggaca acacttcacg cccgccgtac aggcgcggca gcagcgagaa tgcttttgcc
    16381 cacatgccgt ccagcttcca agccttgacc tgcgcttcga ccgccccggt atctttatcc
    16441 tgcatcaagg caagcagcgt tgcggactgc cccgcatcca aatgcgccgc atcggacaag
    16501 gcgcgaaata tgccgatatg ccccagcgaa agcagcactt tgcccatatc ggcaattttc
    16561 atgcttttca gcatcaggtc tatcagctcg atgtcgccac ggatgtcggc aaaaccgtac
    16621 atttccgccc ctgcctgcaa gggttcgcgc atattcagca gaccgtcggg ctgcgcgtgc
    16681 aacaccggac cggcataaca caaccggtta atcccttggt tggcggataa aagatgggca
    16741 tcgatacgcg ccacctgcgg cgtgatgtcg gcgcgtatgc ccaactgcct gccgctgagc
    16801 ctgtccgtta ccaaaatggt tttcagggaa agccccgcat cgatatgcgt cagcagggaa
    16861 tgtgcgtact ccatcagcgg aggctgtacc agttcataac cgtgtacgcg gaacagtgcc
    16921 aacaactgct ccctcgcgct ttcaagctgc cgcgcgttcg tgggcagtac gtcggcgata
    16981 tgttccggaa gctgccatgt ctgcatgaga atctgccttc tattttatcc tgtaaacaca
    17041 aaagactgcc cgatccgcaa tcagacggca tctttgccgc aacgcatcac gccgctgtca
    17101 aaacggcagt gcttccttcc gattcagaat ccgaacagcc cctgtccgac ggctcaatgc
    17161 cgtcaagtcg ttaaaaccaa actttaccat aaaatacaca caatctgaat atacttttat
    17221 accctgtatc cgccgtctga cggcacggca tttttttact atataatgtg caccattatt
    17281 tctcatcact tcctgctgcc gttttctgct tcagacggca tttttgttga ttgaacactt
    17341 atgctgctcg acctcaaccg cttttccttt tccgtcttcc tgaaagaagt ccgcctgctg
    17401 accgctcttg ccctgcccat gctgttggcg caggtcgcgc aggtgggcat cggttttgtc
    17461 gataccgtga tggcgggtgg tgcgggcaag gaagacttgg cggcggtggc tttgggcagc
    17521 agcgcgtttg ccacggttta tattaccttt atgggcatta tggcggcgct gaacccgatg
    17581 attgcccagc tttacggcgc gggtaaaacc gacgaagtgg gcgaaacggg acggcagggg
    17641 atttggttcg ggctgttttt gggcgtgttc ggcatggtct tgatgtgggc ggcgattacg
    17701 ccgttccgca actggctgac cttgagcgat tatgtggaag gcacaatggc gcagtatatg
    17761 ctgttcacca gcttggcgat gccggcggca atggtacacc gcgcgctgca cgcctacgct
    17821 tccagcctga accgcccgcg cctgattatg ttggtcagct tcgcggcgtt tgtgttgaac
    17881 gtgccgctga actatatttt cgtttacggc aaattcggta tgcccgcttt gggaggcgcg
    17941 ggctgcgggt tggcgacgat ggcggtgttt tggttcagct cgctggcatt gtggatttat
    18001 atcgctaagg aaaaattctt ccgcccgttc ggactgacgg caaaattcgg caaaccggat
    18061 tgggcggtgt tcaaacagat ttggaaaatc ggtgcaccca tcgggctgtc ttattttttg
    18121 gaagccagcg cgttttcgtt tatcgtgttt ttgattgcgc ccttcggcga ggattatgtg
    18181 gcggcgcagc aggtcggcat cagtttgtcg gggattctct atatgattcc gcaaagcgtc
    18241 ggctcggcgg ggacggtgcg catcggcttt tcgcttgggc ggcgcgaatt ttcgcgggcg
    18301 cgttatattt cgggcgtgtc actggtgtta ggatggatgc tcgccgtgat taccgtgctt
    18361 tccttggtat tattccgttc gccgctggta agtatgtaca acaatgatcc ggcggtttta
    18421 agcatcgccg ccaccgtctt actgttcgcc ggcttgttcc aaccggcaga cttcacccaa
    18481 tgtatcgcct cctacgcctt gcgcggctac aaagttacaa aggtgccgat gttcatccac
    18541 gccgccgcct tttggggctg cggcctgctg ccgggctacc tgctcgccta ccgtttcgat
    18601 atgggcattt acggcttctg gacggcattg attgcctcgc tcaccatcgc cgccgtcgcc
    18661 ttggtgtggt gcttggaatt gtgcagtagg gagatggtca gatcgcataa ggtcgtctga
    18721 aaacgcagta cacttcaatt caaatacagt taaagttcaa accatgcaac ccatccgata
    18781 ccgaaccgac ctcaccccct acaacacctt cggtcttcgc gcccaagccc gggcctttat
    18841 cgcgctcgaa catgccgacg agttgtgcga catcgtccga ctgcccgagt tcgaccgcga
    18901 tactgtttta tggctgggcg gcggcagcaa catcgttttg atgcaggatt acgccggact
    18961 ggtcgtccat atggaaaaca aaggcatacg cgagattgcg cgttcagacg gcattgttcg
    19021 catcgaagcg caggcgggcg aaatctggca cgattttgtc ctgcacaccg tcgcgctggg
    19081 tttgagcggt ttggaaaacc tgagcctgat tccgggtacg gtcggcgcgt cgcccgtgca
    19141 gaacatcggc gcatacggcg tagaggcgaa agatgtgatt cacagcgtgc gctgcttcga
    19201 tttggatacg gaaacctttg taaccctttc caatgccgac tgccgcttcg cctaccgcga
    19261 aagcctgttc aagcaggaag gcaaagggcg ttatgtgatt gtttcggtcg tatttgcatt
    19321 aaaaacgcat tttgtgccga acttgggcta cggagatttg gcggcgaagg ttgccgaatt
    19381 gagtgcaggc agggaggcga cggcaaaaga cgtttccgat gcagtgtgtg caatccgcaa
    19441 cagtaaactt cctaatccta acgtgcttgg taatgtcggc agtttcttta aaaatcccgt
    19501 catcggcgcg gaaaaagctg ccgccctgtt ggagcagcat cccgatatgc cgcattatcc
    19561 gcagcccgac ggttcggtca aactcgccgc cggctggctg atcgaccaat gtcgtctgaa
    19621 aggctttcaa atcggcggcg cggcggtaca tggcaggcag gctttggtct tagtgaacaa
    19681 aaacaacgcc tcggcaaacg atgtccggca attggcgcaa cacatcaaat ttacagtatt
    19741 tgctcggttt caggtagaat tgcacgccga acccaattgg ctgcctgctt cgttcagcct
    19801 gtaaatccaa ggagtatgcc cgtgacccgc cccgccaaaa tcaatactta cacacgcatc
    19861 atcgacgcca gccttgcgct cttcaacgag gaaggcgagc gcaacatcag caccaaccat
    19921 attgccgccc acttgggcat cagtccgggc aatctctatt accacttccg caacaaagac
    19981 gaaatcatcg tccaactgtt caaacgttac agcgaagccc tgctggcata cctgaatgaa
    20041 gccgtgctgc cgtctgatgt ggaagactct atcaactata tggcgggcat ttacgacgtc
    20101 atgtgggaat accgcttcct ctttagcgac gtaaacaccc tgcttgcacg cagtgccgaa
    20161 ttgttgggcg aacacaacac cttcacccaa gccaaagtct ccccgctctt ggtcaacctg
    20221 ctcacccagc tcaacggact aaacgtcatc aacgccgacc aaaccgctat gaacgacctc
    20281 gccgtcaata tgtggatggt cacgaaatac tggttcgact tcgacagctc cctgcgcggt
    20341 cgcaccaagc tgaccgaaga ctccaaagca cgcggcatcc gccgcacctt aagcctcctt
    20401 cgtccttatc ttttgcccga acaccgcgag aaatacgacc ggaaaatcgg caacggcaac
    20461 ccgtaaaccc acagcgtccg aatcagcctt cagacggcac ttacccgacg gtatcagcga
    20521 atgcgcgcag tccccccgcc ccatatctcc atcacaggcc taggttacct cggcctgccg
    20581 ctggcacaaa agttttacca acacggcagc cgcgtcgccg ccataaaacg cagcctgact
    20641 tcggacgaca tcaacctgcc catacatctc gacaccttcg acctcggcag caccgatgcc
    20701 tttcagacgg cattgtggcg gcaccacgcc gacaaacccg tctggttctt cctgctaccg
    20761 ccctcctcgc tgacgcatta cgccgacacc gtcaaacaat gggcagaact tgcccgggcg
    20821 tgcaacgtgc aacacctgat tttcacaagc agcaccagcg tttacggcga tacagcgcgc
    20881 gaatgcgacg aaaccgccac acccgatccg caaaccgagt ccgcccgcca aatcctcgcc
    20941 gccgaacaac acctgctcga cagcggcatc tccaacatcg acatcctgcg acttggtggg
    21001 ctttattgcg ccgaacgcca tcccgtcagc cgtctcgttc aaaagcaaaa catcccaggc
    21061 ggcaaccgcc ccatcaacat cgtccaccgt aatatcgccg tcgaaagcct gtttcagacg
    21121 gcatttaacc ccggcggcag gcggctgaaa aacattatcg aaccgcgcca cccgacacgc
    21181 cgcgaatttt ttacggaaga agccgccaaa ctcgacctgc ccgcgccgga tttcgcaccc
    21241 gacgacagca ggggcaaaat catccgtacc gtttgcgata acggcttaag cctgtaaaat
    21301 aagcggcaac agcaacaaac aggcattctc cgccccgatc agaaaatatg tccgccctcc
    21361 tccccatcat caaccgcctg attctgcaaa gcccggacag ccgctcggaa cttgccacct
    21421 ttgcaggcaa aacactgacc ctgaacattg ccgggctgaa actggcggga cgcatcacgg
    21481 aagacggttt gctctcggcg ggaaacggct ttgcagacac cgaaatcacc ttccgcaaca
    21541 gcgcggtaca gaaaatcctc caaggcggcg aacccggggc gggcgacatc gggctcgaag
    21601 gcgacctcat cctcggcatc gcggtactgt ccctgctcgg cagcctgcgt tcccgcgcat
    21661 cggacgaatt ggcacggatt ttcggcacgc aggcagacat cggcagccgt gccgccgaca
    21721 tcggacacgg catcaaacaa atcggcagga acatcgccga acaaatcggc ggattttccc
    21781 gcgaatccga gtccgcaaac atcggcaacg aagcccttgc cgactgcctc gacgaaataa
    21841 gcagactgcg cgacggcgtg gaacgcctca acgaacgcct cgaccggctc gaacgcgaca
    21901 tttggataga ctaaccttca gacggcatcc gacatgaaca gcccttttca caacatcggc
    21961 atcgtaaccc gccccaacac gcccgacatc caagacaccg cacacacgct gattaccttt
    22021 ttgaaacagc acggctttac cgtctatctc gacgaagtcg gcataaagga aggctgcatc
    22081 tacacccaag acaccgtcgg ctgccatatc gtcaacaaga ccgaactggg gcaatactgc
    22141 gacctggtcg ccgttttagg cggagacggc acctttctct ccgtcgcccg cgaaatcgcc
    22201 ctgcgcgccg ttccgattat cggcatcaac caagggcatt tgggcttcct gacccaaatt
    22261 ccccgcgaat atatgacgga caagctattg cccgttttag aagggaaata ccttgccgaa
    22321 gagcgcatcc tgattgaggc cgcactcatc cgcgaaggca aaaccgccga acgcgccatc
    22381 gccctcaacg atgccgtcct ctcccgtggc ggcgccggac agatgattga gtttgaagtc
    22441 ttcgtcaatc gggaattcgt ctatacccag cgttcggacg ggctgattgt ctccaccccc
    22501 accggatcga ccgcctattc gcttgccgcc ggcggcccca tcatgcaggc aggattgcac
    22561 gccttcacgc tcgtccccat ctgcccgcaa tccatgacca accgccccat cgccattcca
    22621 gacacgtccg aaatcgaaat cctcgttacc caaggcggcg acgcgcgcgt ccatttcgac
    22681 ggtcaaaccc atatcgacgt gcaaaacctc gaccgcatca ccatccgccg ctaccgaaac
    22741 cccttacgca tcctacatcc gaccgactac caatatttca aaaccctgcg ccaaaaactg
    22801 cactggggcg aacaattggt ctaagccgac ccttaccgga aaacacctat gaatccaaaa
    22861 actccccccg accatacaga agatgccgtg cgcctctcca aacgcatggc gcaactgggg
    22921 ctttgttcgc gccgcgaagc cgacggctat atcgaacagg gctgggtaac ggtcaacggc
    22981 aaaaccgccg tactcggtca gaaagtttta ccggcagacc gtatcgaact aaacaagaaa
    23041 gcccacgaac agcaggcggc acgtattacc atcctgctga acaaacccgt cggctatgtc
    23101 agcgcacaag cggaaaaagg ctataaatcc gccgccgaac tgattacccc cgaaaaccac
    23161 tgggaagacg acaccagccg catccgtttc gatccgaaac acaaaatcgg tcttgccccc
    23221 gccggcaggc tcgatatcga ttcagtagga ttgctggtat tgacgcaaga cggccgtatc
    23281 gccaagcagc ttatcggcga aaacagcggc agtgaaaaag aatatttagt gcgcgtgcgc
    23341 ggcaaattgg acgaaaaagg acttgcctta ctaaatcacg gattgagttt ggacggtgag
    23401 aaactgcgtc ccgccaaagt agaatggcaa aacgaagacc aactgcgttt cgtattgaaa
    23461 caaggtaaaa agcggcaaat ccgccgtatg tgcgaactgg tcggactgcg cgtcgtcggg
    23521 ctgaaacgca tccgcatggg caaggtcaaa ctcggcaggc tgccgcccgg caaatggcgt
    23581 tatctcgctc ccggcgaatc gttttaaata agcattaaaa aatgccgtct gaaaatcctt
    23641 gcgttttcag acggcattgc cttatgccca aataacggtt tcttccaagg gcctgcgcgc
    23701 tttcgtggcg atttcctgaa cgcggtagcc gaatgtcgcg gcaacggaca cgccccattc
    23761 tgccgcatcg aacaaaccga actgcccgga caatacgcgt tccatctcgg catagttgaa
    23821 accttccacc gggcaggaat cgatacctgc catcgccgca cccgtcatca tgttgcccaa
    23881 cgcgatatag gtctggcggc aacaccagtc aaacaaggcg cgcgaatcgt ccaaaatctt
    23941 gatgtcgtcg gcttgaaacg cctgatacct cgccaaagat tttgccatgg catccggttc
    24001 ggtaacgccg cgccgtttga ggctttccaa cataaacggg ctgtcgaagc gggcattttt
    24061 ctttgccaaa aacaccacca aatgactggc ggtatccaaa gcatccgcca taccccaaga
    24121 aaacggcttg attgcctgtc ggatttcggg gttttgaacc acaacaaact gccaaggctc
    24181 cgaaccgacc gaactgggcg acaaacgccc gagttctaaa ataaactgaa aatcctcggc
    24241 actgatttta cgtgcctcat cgtaatgccg gcatgatttg cggtttttaa atgcggatag
    24301 aacctgctct ttgcttaata ctgccatcgc acatcctttc attacttgta ttgcaaattg
    24361 atttcaaacc gatacggcac tgccgcacca tacaatccgt cctcgccatc aagcttcatc
    24421 cggactttac ccatctctct acggctggga aaacaaagcg gcggcagact cgggaataac
    24481 gccccaatgc ctttcagacg gcatttggtt catcgcaaac acgcctacgg gtaggcgata
    24541 taggcataag ccgaatggtt gtggatagat tcgaaattct cactctcgac gacgaaactc
    24601 ttgatgcgtt tgtcggcaat cagcgaagta gcgacatcgc gcaccatatc ttccacgaat
    24661 ttcgggtttt cgtaggcttt ttcggtaacg tatttttcat cggggcgttt gagcaggccg
    24721 tagagttggc agcttgcctg tgcctccaca taatcgatga cttcctcgat accgacttct
    24781 gcatcggcag tcaggctgac ggtaacatgc gaacgctggt tgtgcgcgcc atattgggaa
    24841 atttctttgg aacacgggca aagcgaggtt acgggaatca tgaccttcat actgtagccg
    24901 tatgccccgt ctttcatttc gcccgtgaga cagacatcat aatccagtaa ggaccgaata
    24961 ccggaaaccg gggcggtttt cttgcggaaa aacggaaaag aaacgctgat tttgccggcg
    25021 cgggaatcta aaagcgcgac catttcggca gtcagcctgt gcaattgtgc aaaatccaag
    25081 gcttcagaat gttgctccat caatgcgaca aagcgcgaca tatgcgtccc cttctgctcg
    25141 gcgggcaggt aaaccgtcat ggtcagacgg gcaacggtgg actggatgcc ttctgcagtt
    25201 tgcagggtaa tcggaaagcg caggtctttg ataccgacct gattgatcgg cagattgcgc
    25261 aaatctcggc tggattgcac gtctgcaata gtgttcatga gttgtttttc cttaatattc
    25321 atgtaattgt tgagatgtgt gcatggtatg gcgtttccgc cggggaatat accgtcaatc
    25381 tgcaaatgcg agattatatc actcgctttt agcagctgca ttgattgttt ctatttttca
    25441 gatagtgata aaatcttcga ttattcacat ctacataggc agagaccaaa tgaaattcgc
    25501 cacgaaagcc attcattcca gctacgattg cgacgaacac aaccgcgcgc tgatgccgcc
    25561 gatttatcaa aacagtatgt ttgcgttgca cgagattggc gaaaatgtgc cttaccgtta
    25621 ttcgcgcctg agcaacccga cccgtcagat tttggaagac accgttgccg atttggaaca
    25681 cggtgcggca gggtttgcgt tttccagcgg tatggcgggg attgatgccg tatggcgtac
    25741 cttcctgcaa cagggcgaca ccatcgtcgc ggtcgccgat atttacggcg gcgcgtatga
    25801 cttattggtc gatgtttatc aaaaatgggg ggtgaacgtt gtttttgccg atttgggcaa
    25861 tcccgataat ctggacgagc tgctcaaagc gcacaaggtc aaactggtct ggctggaaac
    25921 gccgtccaat ccgcttttac gcttggtaga catcaaagcc cttgccgcga aagccaaagc
    25981 gaccggcgca ctggtcggta tcgacaacac ctttgccacg ccgtatctgc aacagccctt
    26041 ggatatgggt tgcgattttg tattccattc cgctaccaaa tatttgtgcg gccattccga
    26101 cgtgttgatg ggcgtagtcg ttgccaaaac caaagagctg gctcagcctt tgcacgacat
    26161 gatggtgcat accggcgcga ttgcaggccc gatggactgc tggctggtgc tgcgcggtat
    26221 caaaacgctg gctctgcgca tgaacgccca ttgccaaaac gcactcgaaa tcgcacgccg
    26281 tttggaagcc catcccgcca ttgaaaaagt gttctatccc ggcctgccgt ctcacgaaca
    26341 ttacgaactg gcgaaaacac aaatgcccaa aggcatcggc ggcgtggtta cggtttatct
    26401 caaaaacgac acgcgtgaag cggcaaacag cgtgattaaa aacatgaaac tggtcaaaat
    26461 ggcttccagc ctcggcggtg tggaaagttt ggtcaaccat tgctattccc agtcccacag
    26521 cggcgtaccg catgatgtga aaatggaaat gggcatcaaa gtcggtctgc tgcgtttctc
    26581 catcggcatc gaagacgcgg acgatatttg gaacgatatt tccgccgcac tcgatacaac
    26641 tttgtaaact gtaaaaatgc cgtctgaaac catggtttca gacggcattt caattaaccc
    26701 ggccgaaaat caacgcttca gcatctttgc cgcctcaata gcgtaatagg tcaaaatccc
    26761 gtccgcaccc gcacgtttga atgccagcag gctttccaaa accactttgc cgccgtccag
    26821 ccagccgttg gcaatcgctg cctgcaacat cgcgtattct cccgaaacct gataggcata
    26881 agtcggcaca ccgaactcgt cctttacgcg gcggacaacg tccaaatacg gcaaaccggg
    26941 cttgaccatt accatatccg caccttcctg aatgtccaac gccacttcgt gcaacgcctc
    27001 atcggtattt gccggatcca tctggtaggt ctttttatct gccttgccca aattgcccga
    27061 actgcctacc gcatcacgga aagggccgta aaatgcagaa gcatatttgg cggaatacgc
    27121 cataatccgc gtatggatat gcccggcatc ctccaacgcc tcgcgaatcg caccgatacg
    27181 cccgtccatc atatcggaag gggcaaccac ctgcgcgccc gcttcagcgt ggcacaaagc
    27241 ctgcttgacc aaaacctcta cggtttcatc gttcatcaca taaccgtttt cgtccgtcag
    27301 cccgtcctga ccgtgaaccg tataaggatc gagcgcgaca tccgtcataa tgcccagttc
    27361 gggaaacctc tcgcgcaagg cgcggacagt tgacggcacg agtccttcgg gattgtacgc
    27421 ctcctgcgca cgctcggttt tgtttgccgt aaccacgggg aacagtgcca acatcggaat
    27481 accgagcttt accgcctctt ccgccgtaaa cagcagcctg tccaaacttt gacgcttcac
    27541 acccggcata gaaggcacat cctcctcgcg cgccgacccc tccaatacga acaccggata
    27601 aatcaaatca tcggcggtca gcgtgtgttc gcgcatcagg cggcgtgaaa aatcgtccct
    27661 gcgcatacgg cgcatacgcg aagccggaac attgcggtaa ggaaactgca taagccctcc
    27721 aatcatcctg tcaacaattc aaaccatacg gaagccgccg caacttgcag acagcatcgg
    27781 gcggcaatca aaatccgttt tcaccggaaa gggtttcggc ccgccgaaat gccgcgccga
    27841 aggacggcgt cccgcccatt aaagaacaat agcaaaacaa cagcttcagg ctgtcagcct
    27901 tcgatattct tacgccacaa taccaaaagt ttcccgatat gctgaacagg ttgcgcgtca
    27961 accgcctcac ataaggcagt gcagatttcg atacgttcgg cacggtcgtc gccgaatacg
    28021 cgcactttaa tcagctcatg cgccgtcaat gccgcatcgg tttccttgat gaccgcgtcc
    28081 gtcagaccct gctgaccgac catcacaaca ggatggagat ggtgcgcgcg cgctttcagt
    28141 tccaaaattt ctttggtgtt caatttggta tccgtcattt tcctactgct tgaataaaaa
    28201 gaataatgcg gcattgtacg cgatttggca cgacatcggc aaaattaacg tacaatacgc
    28261 ggtttccatt ccgacccgcg aaccactatg gctgtacgtt ccaaatcctc aaaagcgtgg
    28321 ctgcacgaac acgtcaacga ccactacgtc catatggcgc aaaaagacgg ctaccgcgcc
    28381 cgtgccgcat acaaactttt ggaaatcaac gaaaaagaca aattaatcaa acccggcacg
    28441 gtacttgccg acttgggcag cgcaccggga agctggtcgc aggttgccgc caagctgacg
    28501 ggtacttccg gagcagtttt cgccttggac atcctgccta tggaagccat agggggcgtc
    28561 tccttcattc agggcgactt ccgcgagaac gacgtactgg cacaattcga aaccttgttg
    28621 gacaaccgcc cgctcgacct tgtaatttgc gatatggcgc ccaatatgtc gggaaacgcc
    28681 gtaagcgatc aggcacgcag cttttatctg tgcgaactgg ctttagactt cgcctcgcaa
    28741 cacctgaaaa ccggcggcag ctttttggtc aaagtctttc agggtgcagg ctatcaggaa
    28801 tacatggcag ccatgcgcga aattttcggc acggtgcaga cgcgcaaacc cgaagcctcg
    28861 cgcaatcgct ccagtgagat ttatttattg ggcaaaaata aacgctgaca atacagacgg
    28921 cgtgctttac aatcatttcc gttttacacc tcaattatgg agccttgcta agtggggaac
    28981 acctttaaat caatccttgt ctgggtcgcc ttgggtatcg gcctgatggc tgcgttcaac
    29041 gctttagacg gtaaaaaaga agacaacggg caaatcgaat attctcagtt catccaacag
    29101 gtcaacaacg gcgaagtatc cggcgtcaac atcgaaggat ccgtcgtcag cggctacctg
    29161 attaagggcg agcgcaccga caaaagcacc ttcttcacca acgcgccttt ggacgacaac
    29221 ctgattaaaa cactgctcga caaaaacgtc cgtgtaaaag taacgccgga agaaaaaccg
    29281 agcgcgctgg ctgccctgtt ttacagcctg ctgcccgtcc tgctgctgat tggcgcgtgg
    29341 ttctacttta tgcgtatgca gacgggcggc ggcggaaaag gcggcgcatt ctcattcggc
    29401 aaaagccgcg cccgcctact ggacaaagat gccaacaaag ttacctttgc cgatgtcgcc
    29461 ggctgcgacg aagccaaaga agaagtgcag gaaatcgtcg attacctcaa agcgccgaac
    29521 cgctatcaaa gcttgggcgg gcgcgtgccg cgcggcatcc tgctggcggg cagcccgggt
    29581 acgggtaaga cgcttttggc aaaagccatt gcaggcgaag ccggcgtgcc gttcttcagc
    29641 atttccggtt ccgactttgt cgaaatgttc gtcggtgttg gtgcgagccg cgtccgcgat
    29701 atgttcgagc aggcgaagaa aaacgctccc tgcatcatct ttattgatga aatcgacgca
    29761 gtcggccgcc aacgcggcgc aggtttgggc ggcggcaatg atgagcgcga gcaaacatta
    29821 aaccaattgt tggttgaaat ggacggtttt gagagcaatc agactgtaat tgtgattgcg
    29881 gcaaccaacc gccccgacgt actcgatcct gcgctgcaac gccccggccg tttcgaccgc
    29941 caagtggttg tccccctgcc ggacatccgg gggcgcgaac agattttgaa cgtccattct
    30001 aaaaaagtgc ctttggacaa atctgtggat ttattgtccc tcgcgcgcgg cacgccgggt
    30061 ttttccggcg cggatttggc gaacttggtc aacgaagccg ccctgtttgc cggccgccgc
    30121 aataaagtca aagtcgatca gagcgatttt gaagacgcca aagacaaaat ctatatgggt
    30181 ccggaacgcc gcagtatggt gatgcacgaa gacgaaaaac gtgcgacggc gtatcacgaa
    30241 tccggacacg cgattgttgc cgaaagcctg ccctttaccg accccgtcca caaagtaacc
    30301 attatgccgc gcggacgtgc gctgggtctg acttggcagc ttccggagcg cgaccgcatc
    30361 agtatgtata aagatcagat gttgagccag ctctccatcc tgttcggcgg acggattgcc
    30421 gaagacatct tcgtcggacg catctccacc ggcgcatcaa acgactttga acgcgcaacc
    30481 caaatggcgc gcgaaatggt aacgcgctac ggcatgagcg acaaaatggg cgtgatggtt
    30541 tatgcggaaa acgaaggcga agtcttctta ggacgcagcg taacccgttc gcaaaacatt
    30601 tccgagaaaa cccagcagga catcgatgcg gaaatccgcc ggattttgga cgagcagtat
    30661 caggttgcct acaaaatcct cgatgaaaac cgcgacaaga tggaaaccat gtgcaaagcc
    30721 ctgatggaat gggaaaccat cgaccgcgat caggtactgg aaatcatggc gggcaaacaa
    30781 cccagtccgc ccaaggatta cagccacaac ctgcgcgaga atgcggacgc ggcggaagat
    30841 aacgcgccgc acgctccgac tcgggaagaa accgaagcac ctgccccggc agacaccgct
    30901 tcgacagagt ccgagcagca gcctgaaaac aaggcttaac ttcccgaaca aacggcagcc
    30961 cgcaagctgc cgttttccca tcctgttttc agacggcatt ttccgctcaa tgccgtctga
    31021 aacgccaaag ccgtatcggt ttttgcggat ctgcgatatg gcggtttaaa ttgaaatcag
    31081 acgaggcaag gctgtaccgg tttaaaatta aaccgctata ataccgcctt tcgaatacac
    31141 gcccgcattg gtgatgcctg tgaaaaaagt tgaaaaaaac atcttggtgc tgcacggcgc
    31201 ggacaaaatg ttcgagctgg tcgataaggt tgaagactat ccgcactttc tgccgtggta
    31261 cagcaagacc gaggtcatcg ggcgtagcgg caacgaactg aaggcgcggc tgtttatgga
    31321 ctatatgcac gttcgccaat cattcgccac gcacaaccgc aacattccgg gcagggaaat
    31381 ccgtatggaa ctgctcgagg gtccgttcaa aaccttacgt ggaacgtgga aatttatcga
    31441 tttgggcgac gatatgtgca aaatcgaatt caatttggaa tacgattttt ccaatgccgt
    31501 tttgtccgcc ttaatttccc ccgtcttcaa ccacctttcc gccacgctgg tcgaagcgtt
    31561 cgtcaaagag gcagaccgcc gttatgcttg aaattgagat tgtgtacggg ctgcccgacc
    31621 gacaggtttt gaaaaccatg cagcttgccg agggaacaac cgtccgcgcc gccgcactgc
    31681 aaagcggttt ggacggcata tttgaagatt taaacctgca ttccgcgcct ttgggcattt
    31741 tcggcaaagc cgtcaaagac gacacgcccc tgcgcgacgg cgaccgcatc gaagtgtacc
    31801 gcccgctgtt gatcgacccc aaagaagcgc gccgcaaacg cgttcaaaat caagaagaat
    31861 aaccatgccg tctgaagcct tcagacagca ttcgacagaa acacggaaaa tatcatgtca
    31921 aacaaaatca aaatgcttgt cggattgggc aaccccggca aagaatacga acaaacccga
    31981 cacaatgcgg gtttttggtt cctcgacgaa ctggcgtgga aatggaaggc ttcatttaaa
    32041 gaagaaaaaa aattcttcgg cgaagtcgcc cgtgccgccc tgcccgacgg cgacgtttgg
    32101 ctgctcaaac ctgccacgtt catgaaccgt tccggacagg cagttgccgc gcttgcacag
    32161 ttctacaaaa tcaaacccga agaaatcctc gtcgtccacg acgaactcga cattccctgc
    32221 ggacggatta aattcaaact cggcggcggc aacggcggac acaacggctt gaaagacatt
    32281 caggcaaaac tcggcacggc agactattac cgcctgcgcc tcggtatcga ccacccgggc
    32341 gaccgcaacc tcgtcgtcgg ctatgtcctg aacaaaccca gtacggaaca ccgccgacag
    32401 attgacgatg ccgtcgccaa atccctgcaa gccatacccg acatccttgc cggcaaatgg
    32461 gaagaagcaa cccgcttcct gcacagcaaa tgacccgatg ccgtctgaag ccctttcaga
    32521 cggcatgttc ccgatttcca tatccgaaca gtcatgaccg aactcaagca gcttatccaa
    32581 accgaatcca tccccgtcat cgaagaaacc ctcgatttcc tgctgtacga atgcagcatc
    32641 gacgacgcac catccgccga agaagtggca caatggcgcg acatacttgc cgcacgcggc
    32701 ggcaaattcc tgcgcctgtc caaaatctgc caaacgtggc tggacgagga ggcggcatga
    32761 agctgccgcg caaccgcttc agcctgcttt ccgcattgtg gtttgccggc ggcatctatt
    32821 cgctgctctt caaagctgcc gacaccgcgc cgccgccgtt tccgcatttc gacaaagcag
    32881 cacaccttgc cctgtttttc gcacaaatct ggcttttgac caaagcattc aaaaccggaa
    32941 aacttcccat cccctaccgc agcctgatgg tctttgccct ctgtttcgcc ctcttcagcg
    33001 aatgcgcgca ggcatggttt accgcaacga gaaccggcag tttgggcgat gttcttgccg
    33061 atatggcagg tacggttctc gcactctttg ccgcccgcgc cgccgaccgc ccggactgaa
    33121 tcggtttatt tcccccaaac aggatgcacc tcttcccgat acatcctatt tttcccctta
    33181 catatcaatc ccatctcata aacaaaaccg acaaatcgtt tacaatatat ttacactaca
    33241 tcagattaca aatatactcg aaccagttgt agaatcggcg gttccacaaa ccgtttcgga
    33301 ttgcacgaca caaaagaaaa accgatttta ttgccttaaa ggagtattca tgaacctgca
    33361 tgcaaaggac aaaacccagc atcccgaaaa cgtcgagctg ctcagtgcgc agaagccgat
    33421 taccgacttt aagggcctgc tgaccaccat tatttccgcc gtcgtctgtt tcggcattta
    33481 ccacatcctg ccttacagcc ccgatgccaa taaaggtatc gcgctgctga ttttcgttgc
    33541 cgcactttgg tttaccgaag ccgtccacat taccgtaacc gcactgatgg tgccgattct
    33601 cgccgtcgta ctcggtttcc ccgacatgga catcaaaaag gcgatggctg atttttccaa
    33661 cccgattatc tacatttttt tcggaggctt cgcgcttgcc accgccctgc atatgcagcg
    33721 gctggaccgt aaaatcgccg tcagcctgtt gcgcctgtcg cgcggcaata tgaaagtggc
    33781 ggttttgatg ttgttcctcg ttaccgcctt tctgtccatg tggatcagca acaccgccac
    33841 cgccgcgatg atgctgcctc tagcaatggg tatgctgagc cacctcgacc aggaaaaaga
    33901 acacaaaacc tacgtcttcc tcctgctcgg catcgcctat tgcgccagca tcggcggctt
    33961 gggcacgctc gtcggctcgc cgcccaacct gattgccgcc aaagccctaa atctggactt
    34021 cgtcggctgg atgaagctcg gcctgccgat gatgctgttg attctgccct tgatgctgct
    34081 ctccctgtac gtcatcctca aacctaattt gaacgaacgc gtggaaatca aagccgaatc
    34141 catcccttgg acgctgcacc gcgtgatcgc gctgttgatt ttccttgcca cagccgccgc
    34201 gtggatattc agctccaaaa tcaaaaccgc cttcggcatt tccaatcccg acaccgttat
    34261 cgccctgagt gccgccgtcg ccgtcgtcgt cttcggcgtg gcgcaatgga aggaagtcgc
    34321 ccgcaatacc gactggggcg tgttgatgct cttcggcggc ggcatcagcc tgagcacgct
    34381 gttgaaaaca tccggcgcgt ccgaagcctt gggacagcag gttgccgcca ccttttccgg
    34441 cgcgcccgca tttttggtga tactcatcgt cgccgccttc attatttttc tgaccgagtt
    34501 caccagcaac accgcctccg ccgcattgct tgtaccgatt ttctccggca tcgctatgca
    34561 gatggggctg cccgaacaag tcttggtatt cgtcatcggc atcggcgcat cttgtgcctt
    34621 catgctgccg gttgccacac cgcctaacgc gattgtgttc ggcacgggct taatcaagca
    34681 acgcgaaatg atgaatgtcg gcatactgct gaacatcctc tgcgtagtat tggttgctct
    34741 gtgggcttat gctgtactga tgtaaaccat cgacctaaac aacaagaccg tctgaaagaa
    34801 tatttttcag acgaccttga agtttgtcgt tcagacacaa tttgtcgaat cattcaaaac
    34861 cggattctaa cgaaaggaaa cccatgatta tcctgcacac caacaaaggc gacatcaaaa
    34921 tcgaactcga tttcgacaaa gcccctgtta ccgccaaaaa cttcgagcaa tacgtcaaag
    34981 acggcttcta cgacggcgta atcttccacc gcgtcatcaa aggcttcatg attcaaggcg
    35041 gcggcatgga tgaaaacatg aacgaaaaag aaacccgcga tccgattcaa aacgaagcgt
    35101 ccaacggcct gcccaacgat aaatacacca tcgccatggc acgcacttcc gacccccatt
    35161 ccgccagcgc gcaattcttc atcaacactg ccgacaacgc tttcctgaac ttccgttcta
    35221 aagagctgta cggcaaaacc atcgtccaag actggggcta tgccgtattc ggtaaagtcg
    35281 ttgacggttt tgacgttgtc gatgccatcg aaggcgtttc taccaaacgt catggttacc
    35341 acgacgacgt accgagcgaa cctgtcgtca tcattaaagc cgaagcggta taaaccgaca
    35401 atccgaaagc agcctgcaca aaggctgctt ttatttcaga cggcatgtta tttgatgtaa
    35461 ccgtgctatg ccgtctgaaa accggacgca agccttcaga cggcataccg ctatgttaaa
    35521 atatgcccgt ttttcccaaa cggacacaaa ggaaacctta tggcaaatat tgcccgcgac
    35581 atcttcaaag cctacgacat ccgaggcatt gtcggcaaaa ccctgaccga cgatgccgcc
    35641 tatctcatcg gcagggccat cgccgccaaa gccgccgaaa aaggcatgac ccgcatcgcg
    35701 ctcggacgcg acggacgctt gagcggtccc gaactgatgg agcacatcca acgcggcttt
    35761 accgacagcg gcatcgacgt cctcaacgtc ggtatggtcg ccacgcccat gctctacttc
    35821 gcagccatca acgaatgcgg cggcagcggc gtgatgatta ccggcagcca caatccgccc
    35881 gattacaatg gtttcaaaat gatgctcggc ggcgacacgc tcgcaggcga agccattcaa
    35941 gaacttttag ccattattga aaaagacggt tttgttgccg ccgacaaaca aggcaacgta
    36001 accgaaaaag acatctccgg cgaataccac aaccacatcg tcggccacat caaactcaaa
    36061 cgccccatga aaatcgccat cgacgcaggc aacggcgtag gcggcgcatt cgcaggcaaa
    36121 ctctacaaag gcttgggcaa tgaagttacc gaacttttct gcgaagtgga cggcaacttc
    36181 cctaatcacc accccgaccc ttccaaaccg aaaaacctgc aagacctcat cgtcgagctc
    36241 aaaaacggcg aggccgaaat cggcttggcg tttgacggcg atgccgaccg cttgggcgtg
    36301 gttaccaaag acggcaacat catctatccg gaccgccaac tgatgctttt cgcgcaagac
    36361 gtgttgagcc gcaatcctaa agccaaagtg attttcgacg tcaaatccac ccgtctgctt
    36421 gcgccttgga ttaaagcaca cggcggcgaa cctgtgatgg aaaaaaccgg ccacagcttt
    36481 atcaaatccg ccatgaaaaa aaccggcgcg ccggttgccg gcgaaatgag cggacacatc
    36541 ttcttcaaag aacgctggtt cggcttcgac gacggtctgt acgccggcgc acgcctcttg
    36601 gaaatcctgt ccgcctccga taatccgtcc gaagtgttaa acaacctgcc gcaaagcatt
    36661 tccactccgg aactcaacat tgacctgccg gaaggcagca acggccacaa agtgattgaa
    36721 gagctggctg ccaatgccaa gtttgaaggt gcgaccgaaa tcatcaccat cgacggcttg
    36781 cgcgttgaat tccccgacgg cttcggcctg atgcgtgctt ccaataccac gccgattctg
    36841 gtgttgcgtt ttgaggcgga tacgcaagca gccatcgagc ggattcaaaa ccaattcaaa
    36901 gccgtcatcg aaagcaatcc gaatctaatc tggcctctgt aaacacagga aaaatgctta
    36961 atcaaatctt ggtagattgg atttggctta aaaatctccg cgtccgttcg tgtttggggt
    37021 ggtcgaacaa atcttgcggg ctgccttgtt cgacaataac gccgccgtcc atcacgacga
    37081 cggtggttgc cacttctaag gcgaacttga tttcatgcgt aacgacaacc atcgtccaac
    37141 cttcccgcgc caattccttc atggcgttca acacgtcttg caccaactcg gggtcaagcg
    37201 cggaagtggg ttcgtcaaac aacatcagct cgggctgaat cgccaatgcg cgggcaatgc
    37261 cgacgcgctg ctgctgaccg ccggaaagct ggtagggata caaatccact ttgtcgccca
    37321 agccgacttt ttccagcagt ttcagagcct cttcgcgcgc ttgggcggca ggcttgccct
    37381 gtacggcaac cggtccttcc atcacgtttt ccaaggcggt tttgtgcgga aagaggttgt
    37441 attgttgaaa caccatgcct gatttgcggc gcagtgccaa aatatcgtgt ttgcttggtt
    37501 ttttagaaaa atcgattttc agcggtcgct cgttgtcgaa ctcgatttgt ccgtcttcgg
    37561 gcatttccaa cgcgtttagg cagcgcagaa acgtcgtttt gcctgagccg gaaggcccga
    37621 ggatgacgac cacctgccct ttgcacacat ccaaatcgat gccgcgcaaa atggtatttt
    37681 cgccgaaggt cttatggata ttgcggattt taatcatgac aactcctcat ttggcgacat
    37741 agcggtcgaa acgtttttcc aaacgcgcct gaatcagaaa cagcactttg cagaaacacc
    37801 agtaaaccaa agcggcttcg atatagacgg gcaaaaagtc ataagtgcgg tttgccgtct
    37861 cttgggcgac acgaaaaagc tctgttaccg ttaccaccgc cgcgagcgag gtgtttttga
    37921 acaagccgat aaactcgttg ctcaaaggcg gcacggcaac gcggaatgcc tgcggcgcga
    37981 cgatgcggcg gaacgtctgc atataggtca taccgatgga gaaaccggct tcccattgtc
    38041 ctttcggtac ggacaaaatt gccgcgcgta tggtttcgga agcatatgcg ccgacattga
    38101 gcgagaagcc gataatggcg gcaggaatcg gatcgatata gataccgacg gacggcagtc
    38161 cgtaaaacac aatcacaagc tgcaccaaca gcggcgtacc tcgaatgacg gaaatataaa
    38221 attccaccaa tttcagcagg attttccgcg caatgccgcc ggcgggcata atccgcacca
    38281 aagccacggc taccgcaatc atcataccga taacgaaaga agctgccgcc aaaggcagag
    38341 acaccgcgaa gccggctttg accataggca aaaacgcgct gacaatcata tcggcgcgtg
    38401 tttccgtcat aaacggcagc gaagcaagga aattattgaa cactgatgtc ttttccgaag
    38461 aattgttcgc ccagtttttt cagcgtaccg tcggctttca gctcgttgat tgccgtactg
    38521 aatttcgcca cggcttcgtc attgcccttg ttgacaatca ggccggaacc gactttttca
    38581 tcggcaggtg cggaccaaac gattttcacg cccgcattcg ggtttttctt cagatagtcc
    38641 aaaaccgcca attcgtcgtt cagggttgca tcggcacgtt tttgttcaat cagggtcagc
    38701 gattgcgcca aaccgtcaac agccaccaaa tctgcgcctg cagctttggc tttttcgccg
    38761 tagttgctgg tcagggattg tgcggttttc acgcctttga tgtcgtcgat agatttgatg
    38821 ttgctgtcgt tacgggcaac caatacggca ccgctccagc tgtaaggatc ggatttgtcg
    38881 aatgtcgctt ggcgttcggg gctggtcaga ccgacttggt ttgccaccac gtcgaaacgc
    38941 cccgccttca aacccgccat catcgaatcc cattgcgttt ctttaaactc gactttcacg
    39001 cccagttttt ccgccacggc gcgggttact tccacatcgt aaccggtcag tttgccgtcc
    39061 ttgtcgtggt aggtaaacgg tgcgtaagtg ccttccgtgc cgacggtaac cgtgccttta
    39121 ttgttgatgc gctcgattaa agaaccggaa actgccgatt gtgcaggcgc ggaagatgct
    39181 ccgctgccgc cttccgaacc gccgcaggcc gccaaaacca atgcggcaat accgccgagt
    39241 acgaattttt tcaacatcat tctctcctgt aaaaagtgtg caagtgcggc attctaagtg
    39301 aaattgcggc aaacacaaag aatacttttt ttatatggtt attccaaatc ggaatggaaa
    39361 agatatgccg aagccgccgg aagcggaaat aaagggggcg ggaaaggcgg cgggcggcac
    39421 gaaaccgaaa agcggcatca ggtatgcaaa cggcggattc acgcacgccg ttccggcaaa
    39481 aatccgcctg ccgattcggt gctataatcc atccgaaaca gatgacacca aaaaggaaca
    39541 tctcatgaac acgccccaat ccgccatcat tcccgaccac gcccaagccg gcatctttat
    39601 cgaagccgac ttcgccgcca accgcctcaa cgatattaaa tccgcctgcc gcgcttcgct
    39661 cgacgcgtta tccgccttga aaacccgctt tccggacgat attttgggtc tgaccatcgc
    39721 cttcggcagc aaagcctggg caacattcgg acatacggac gaaggcagtg aaatcaaacc
    39781 cttctccgaa atgggcaacg ggcttgcgcc gtctacgcag cacgatatgt ccatccacat
    39841 ccaatccttc cgccaaaacg ccgcctacgc gcttgcccaa tccgttttgg gcgcgttcgg
    39901 cgacagcata tgcgtcgcgt ccgaagaaca cggtttgcgc ctgtatcagg atcgcgggct
    39961 ggacggtttc gtcgacggca cggaaaaccc gcagggcgat gaaaatgtcc gcgaagtcgc
    40021 catcatcccc gaaggaaaac ccgatgcggg cggcagctat gtcctgctgc aaaaatacct
    40081 gcacgatttg aaaaaatggg atgccgtccc cgtcgccgaa caggaggcct cggtcggacg
    40141 cggcaaggaa accgacgacg aattcggccg cgatgtccgc ctgcccgatt cgcacctcgg
    40201 ccgcgtcaac ctgaaagaaa acggagtcgg cctgaaaatc gtccgccgca gcctgccctt
    40261 cggcaaaatc agcggcgaac acggtttgat gtttaccgca tactgccgca cgctgcacaa
    40321 tatcgaagcg caattattga gtatgttcgg cgatacggac ggcaaaaccg acctgctgct
    40381 ccgacatctc tccgccgccg tttcgggcgg atactactac gcgccgtctg tcgaacgcct
    40441 gcaaaacctc taagccggac gatgccgtct gaaaccattt ttcagacggc atttt
//
LOCUS       DAXHKX010000004        32214 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHKX010000004 DAXHKX010000000
VERSION     DAXHKX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.004"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            234..512
                     /locus_tag="ACFMWZ_000207"
     CDS             234..512
                     /locus_tag="ACFMWZ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934233.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
     gene            531..1679
                     /gene="earP"
                     /locus_tag="ACFMWZ_000208"
     CDS             531..1679
                     /gene="earP"
                     /locus_tag="ACFMWZ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC5934234.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWQQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQNALQNDGDVFQTASVCLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTSETASAHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            1722..2282
                     /gene="efp"
                     /locus_tag="ACFMWZ_000209"
     CDS             1722..2282
                     /gene="efp"
                     /locus_tag="ACFMWZ_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC5934235.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(2332..3177)
                     /locus_tag="ACFMWZ_000210"
     CDS             complement(2332..3177)
                     /locus_tag="ACFMWZ_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC5934236.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(3294..3875)
                     /gene="metW"
                     /locus_tag="ACFMWZ_000211"
     CDS             complement(3294..3875)
                     /gene="metW"
                     /locus_tag="ACFMWZ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC5934237.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            complement(3872..5011)
                     /locus_tag="ACFMWZ_000212"
     CDS             complement(3872..5011)
                     /locus_tag="ACFMWZ_000212"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC5934238.1"
                     /translation="MSQNASVGIVTPQKIPFEIPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGKHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALTDYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            5169..5369
                     /locus_tag="ACFMWZ_000213"
     CDS             5169..5369
                     /locus_tag="ACFMWZ_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934239.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRKQKRALRDVAKISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            complement(5474..5599)
                     /gene="ykgO"
                     /locus_tag="ACFMWZ_000214"
     CDS             complement(5474..5599)
                     /gene="ykgO"
                     /locus_tag="ACFMWZ_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC5934240.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(5599..5874)
                     /locus_tag="ACFMWZ_000215"
     CDS             complement(5599..5874)
                     /locus_tag="ACFMWZ_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC5934241.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            6073..6951
                     /gene="metF"
                     /locus_tag="ACFMWZ_000216"
     CDS             6073..6951
                     /gene="metF"
                     /locus_tag="ACFMWZ_000216"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC5934242.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            7087..9363
                     /gene="metE"
                     /locus_tag="ACFMWZ_000217"
     CDS             7087..9363
                     /gene="metE"
                     /locus_tag="ACFMWZ_000217"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC5934243.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVNAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            9641..10378
                     /locus_tag="ACFMWZ_000218"
     CDS             9641..10378
                     /locus_tag="ACFMWZ_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC5934244.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            10627..12030
                     /locus_tag="ACFMWZ_000219"
     CDS             10627..12030
                     /locus_tag="ACFMWZ_000219"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC5934245.1"
                     /translation="MKKIQADVVVLGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKVVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            12309..12686
                     /gene="sdhC"
                     /locus_tag="ACFMWZ_000220"
     CDS             12309..12686
                     /gene="sdhC"
                     /locus_tag="ACFMWZ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC5934246.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            12680..13021
                     /gene="sdhD"
                     /locus_tag="ACFMWZ_000221"
     CDS             12680..13021
                     /gene="sdhD"
                     /locus_tag="ACFMWZ_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC5934247.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            13024..14787
                     /gene="sdhA"
                     /locus_tag="ACFMWZ_000222"
     CDS             13024..14787
                     /gene="sdhA"
                     /locus_tag="ACFMWZ_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC5934248.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDNQT
                     GGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            14907..15614
                     /locus_tag="ACFMWZ_000223"
     CDS             14907..15614
                     /locus_tag="ACFMWZ_000223"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC5934249.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            15618..15866
                     /locus_tag="ACFMWZ_000224"
     CDS             15618..15866
                     /locus_tag="ACFMWZ_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC5934250.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            15978..17261
                     /gene="gltA"
                     /locus_tag="ACFMWZ_000225"
     CDS             15978..17261
                     /gene="gltA"
                     /locus_tag="ACFMWZ_000225"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC5934251.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            17466..20294
                     /locus_tag="ACFMWZ_000226"
     CDS             17466..20294
                     /locus_tag="ACFMWZ_000226"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC5934252.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADAYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWGKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQNREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALVL"
     gene            20394..21593
                     /gene="odhB"
                     /locus_tag="ACFMWZ_000227"
     CDS             20394..21593
                     /gene="odhB"
                     /locus_tag="ACFMWZ_000227"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC5934253.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAPA
                     VALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKF
                     EKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVP
                     ILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINP
                     PQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDP
                     ARLLLDL"
     gene            complement(21648..21761)
                     /locus_tag="ACFMWZ_000228"
                     /pseudo
     CDS             complement(21648..21761)
                     /locus_tag="ACFMWZ_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            21958..23391
                     /gene="lpdA"
                     /locus_tag="ACFMWZ_000229"
     CDS             21958..23391
                     /gene="lpdA"
                     /locus_tag="ACFMWZ_000229"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC5934254.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            23516..23803
                     /locus_tag="ACFMWZ_000230"
     CDS             23516..23803
                     /locus_tag="ACFMWZ_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934255.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            23857..25068
                     /gene="sucC"
                     /locus_tag="ACFMWZ_000231"
     CDS             23857..25068
                     /gene="sucC"
                     /locus_tag="ACFMWZ_000231"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC5934256.1"
                     /translation="MEADSGHSTNLKEKSMNLHEYQAKELLASYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            25079..25969
                     /gene="sucD"
                     /locus_tag="ACFMWZ_000232"
     CDS             25079..25969
                     /gene="sucD"
                     /locus_tag="ACFMWZ_000232"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC5934257.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            25998..26213
                     /locus_tag="ACFMWZ_000233"
     CDS             25998..26213
                     /locus_tag="ACFMWZ_000233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934258.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFWGSQRPLQNSPK
                     SPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(26520..28115)
                     /locus_tag="ACFMWZ_000234"
     CDS             complement(26520..28115)
                     /locus_tag="ACFMWZ_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HFC5934259.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(28149..30998)
                     /gene="uvrA"
                     /locus_tag="ACFMWZ_000235"
     CDS             complement(28149..30998)
                     /gene="uvrA"
                     /locus_tag="ACFMWZ_000235"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC5934260.1"
                     /translation="MCKYHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMESGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFASVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            31153..31950
                     /locus_tag="ACFMWZ_000236"
     CDS             31153..31950
                     /locus_tag="ACFMWZ_000236"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC5934261.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQAAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
BASE COUNT         8013 a         7917 c         8159 g         8125 t
ORIGIN      
        1 atttcaaacc gacgcgccgg gttttcaata tgcccgcgcc cgatgcagcc ttgtccgcag
       61 gcatcggcgg cagcgtccga tttttcgggg aacgcccgtc tcgggcgtat ttaaaggttc
      121 ggcggtgcgg tgttttcctg cggcaaggct tcagacggca tcgccggcgc gtccgttaga
      181 caaggcgtgc gcttggggcg ataatggcgt tttgcttttt tgaaagcctt gcaatgtccc
      241 gaaacctgct tgtccgctgg cttgtcgtct gcctcatccc tttggcgaca cttgccgttt
      301 tcgtcgccaa tccgcccgaa gacaaacccc agcatctgat caacggcatc atccttgcct
      361 gcgaagcgac gtttttgttc aaattcgtcc ttttcgacac catcaagcat catttgaaac
      421 aagagtttga tttgaaacgt caaaccatgt tgctgtttat tccgattgtt ttgctggttg
      481 tgtatttgtt ccactatttc ggcgcgtttt agcttgtttc cgttatttct atgaatactc
      541 ctccttttgt ctgttggatt ttttgcaagg tcatcgacaa tttcggcgac atcggcgttt
      601 cgtggcggct cgcccgtgtt ttgcaccgcg aactcggttg gcaggtgcat ttgtggacgg
      661 acgatgtatc cgccttacgt gcgctttgcc ctgatttgcc cgatgttccc tgcgttcatc
      721 aggatattca tgtccgcact tggcattccg atgcggcaga tattgatacc gcgcctgttc
      781 ccgatgtcgt catcgaaact tttgcctgcg acctgcccga aaatgtgctg cacattatcc
      841 gccgacacaa gccgctttgg ctgaattggg aatatttgag cgcggaggaa agcaatgaaa
      901 ggctgcatct gatgccttcg ccgcaggagg gtgttcaaaa atatttttgg tttatgggtt
      961 tcagcgaaaa aagcggcggg ttgatacgcg aacgcgatta ctgcgaagcc gtccgtttcg
     1021 atagcgaagc cctgcgtcag cggctgatgc tgcctgaaaa aaacgccccc gaatggctgc
     1081 ttttcggcta ccggagcgat gtttgggcga agtggctgga aatgtggcaa caggcaggca
     1141 gcccgatgac cctattgctg gcgggtacac aaatcatcga cagcctcaaa caaagcggcg
     1201 tcattccgca aaatgccctg caaaacgacg gcgatgtttt tcagacggca tccgtctgcc
     1261 ttgtcaaaat ccctttcgtg ccgcaacagg acttcgacaa actgctgcac ctcgccgatt
     1321 gcgccgtcat tcgcggcgaa gacagtttcg tgcgcgccca gcttgcgggc aaacccttct
     1381 tttggcacat ctacccgcaa gatgaaaacg tccatctcga caagctccac gccttttggg
     1441 ataaggcaca cggtttctac acgtccgaaa ccgcatcggc acaccgccgc ctttcagacg
     1501 accttaacgg cggagaggct ttatccgcca cgcaacgcct cgaatgttgg caaaccctgc
     1561 aacaacatca aaacggctgg cggcaaggcg cggaggattg gagccgttat cttttcgggc
     1621 agccttccgc atccgaaaaa ctcgccgcct ttgtttcaaa gcatcaaaaa atacgctaga
     1681 atagcgcgtt ttacgacaac cgatttgatt ggaaaatcac aatgaaaaca gcacaagaac
     1741 tgcgcgccgg caatgtattt atggtcggca acgatcctat ggtcgttcaa aaaaccgaat
     1801 acatcaaagg cggccgctct tccgccaaag tcagcatgaa actgaaaaac ctgctgaccg
     1861 gcgcggcttc cgaaaccatt tacaaagccg acgacaaatt cgacgtggtc atcctgtccc
     1921 gcaaaaactg tacgtacagc tactttgccg acccgatgta cgtctttatg gacgaagaat
     1981 tcaaccaata cgaaatcgaa gctgacaaca tcggcgacgc gttgaaattc atcgttgacg
     2041 gtatggaaga ccaatgcgaa gttaccttct acgaaggcaa ccccatttct gtcgaactgc
     2101 ctaccatcat cgtacgcgaa gtcgagtata ccgagcctgc cgtcaaaggc gatacttcag
     2161 gcaaagtgat gaaaaccgcg cgcctggtcg ggggcaccga aatccaagtg atgtcttaca
     2221 tcgaaaacgg cgacaaaatc gaaatcgata cccgcaccgg cgaattccgc aaacgcgctt
     2281 gatttgttgc attgaaaaat gccgtctgaa aacgtttcag acggcatttt tttatcttcg
     2341 ccccaagttt ggattgaagt agatgttttt ttcgtaaacg acaatacgcg tgattttgcc
     2401 attttcgtca aaatggatgt ctaaaaacgg tttgtcggga ttgcgttcac ggttggacag
     2461 ccggaagagt gattggtttt caagcatttc tccgtgtatt ttcagatagc cgggcaattg
     2521 gccgatgtat tggaaattgc cgccaagtcc gttgttgtgc cggctcgaat aggcaggggt
     2581 tgccgaaact tcaaaataac gcgtgcgttt cggctcctcg ccttgtccgc gggtttcttg
     2641 actgccctgt atcagcaaca gcgtcgcttc tcggaggcgg cgttcctgtt ccatcaaggc
     2701 atcttgtgcc tgccggctga tcgtgcgctt tccgtaacgc aagtcttcaa ttgccagtat
     2761 gattttctga tggtagtcgg gatcttcagg gcggatgtcg ggaaacagca gccgctgtat
     2821 gttgtcatag ccgccgccgg gtaagccgaa acgggtttcc agttcgtgat gggaaagggc
     2881 aaacagacag tccgagtcaa tgtgttcggc gatttgctgc atgagatcgt caatgccggt
     2941 ggcggggtgc aggtcggtgc aatttgacgg cggcacgctt tctgcctgct tgacgttatc
     3001 aggggcggat gcggccggtt gcaaatcggt ttggggcgcg gaagacgaag cggcggatgc
     3061 cgtggacgag gcggcggatg cgccgttttc tttcgtttta tcttctttgc tcggggaaca
     3121 cgcggtcagc atcattgcgg caagccataa gagaagtgat gaggttttgt ttttcattct
     3181 attgtttcca gtattaaaga ggccgtctga aaacctaccg tttcattttt cagacggcct
     3241 gttgttaata gaaccgaaga acctgttaat gccgacaagg ttctcaatct gtcttacccg
     3301 acgcggtaaa atgcaaggct gcccaaaagg ttggggaaat gtttgacctg tctgttgccc
     3361 gtcatcacgg cgcgctcaag cacgcggatg ttgtttttgg cgcacaacaa atcaaagtct
     3421 ttgagcgtgc accaatggat attgggcgtg tcgtaccaat ggtagggcat acgttcggaa
     3481 accggcatat gtccgccgag tgcgatttgg acgcggttgc gccagtagcc gaaattcggg
     3541 aagctgacga ttgcctgttt tgccacgcgc atcaggcagc gcaggatttt ttcggtattc
     3601 tgcatcgctt ggatggtctg gctcaacaca atcacatcaa aactttgatc gttgaatgcg
     3661 gttaaacctt cttccaaatc ggcttggata acattcacgc cgcgcgacat cgcggcaatc
     3721 acgctgtcgg tgtcgatttc gatgccgtag ccgctgcatt ttttgtgttc gaccaaggcg
     3781 gcaagcagtt cgccgtcgcc gcagcccaag tccaagacgc ggctgccttc gggtatccgg
     3841 tcgtaaatca gttgcaaatc atcgcgcagg ttcattgctg acattcctta taaacgttgt
     3901 tcatataggc ggcgaccgca cgcatatagg cttcgtcttc cattaaaaag gcatcgtgcc
     3961 cgtgtgcgga tttgacttcg atatactgca cggatttttg ggcggcaatc agggccttga
     4021 ccagttcgtg cgaacgttcg ggcgcgaaac gccaatcggt gctgaagctg gcgacaaaga
     4081 attttgcctg aacatcctgc acggcgcggg tcaggctgtt gccgaaatcc gccgccggat
     4141 cgaaatagtc caaagccttg gtcatcagca aataggtgtt ggcatcaaac cgcccgacga
     4201 atttgtcgcc ttgatagcgc agataggatt ctacttcaaa ttcaacgcca tagccgtatt
     4261 gatagccgtt ggaacgcaaa tcgcgtccga attttttgcc caaaccgtct tcggcaagat
     4321 aggtgatgtg ccccatcatg cgggcaatcc gcaagccccg agcgggaacg gtgttgcggc
     4381 tgcggtaatg tccttcgttg aaatcgggat cggtcaaaat cgcctgacgt gctacatcat
     4441 taaacgcgat attttgcgtg gacagtttcg gcgcggacgc aatcactaag gcatggcgca
     4501 cgcgttcggg ataggaaatc gcccactgca aagcctgcat accgcccaaa ctgccgccga
     4561 caaccgccgc ccattgcccg atgccgagat aatcggtaag cgcggcttgg gattttaccc
     4621 agtccttcac cgtaaccacc ggaaaatccg cgccgtattc cctgcccgtt tcaggattga
     4681 tcgacaaagg tccgctgctg ccgtcgcagc cgcccagatt gttcaaaccg accacgaaaa
     4741 atcgttccgt atcaatcggt ttgccgggac cgaccatatt gtcccaccag cccgtatatt
     4801 tatcctccgc cgaatgtttg cccgcaacat gatggttgcc cgacagcgca tggcagatta
     4861 aaaccgcatt gtttttttcg gcattcagct cgccgtaggt ttcaatcatc agatcgaaac
     4921 gcggcaaagt tttaccgttt tccaaaacca gcggtatctc aaacggaatt ttttggggcg
     4981 ttacaatgcc caccgaggca ttttgactca tatcctgttc caacaaatgc ggcgaaaagc
     5041 gttattatat cgcaaacggc atgacttttt gacacggtcg gacaagcagc ccgacgcgct
     5101 gaaacatccg tccccatgcc gccgcaaccg atccatactt tttcagacga cctccaccgc
     5161 ttccctatat gataggcaga cttttccgta tttttttctt tttcgcactt gccgcgctga
     5221 ttatcaaccg cctcttcagc cgcaagcaaa aacgtgccct gcgcgatgtc gccaaaatca
     5281 gcgcatgggt actgcttggc gcagctgccg cgacactgtt ttggtatctg tttatgctgt
     5341 atttcaaaca cattccggat tcgtattgac ggaaaaaatg ccgtctgaaa cgcatttttc
     5401 tgtttcagac ggcatatttg aaaagggctt gcggtaggag gggctttaca accgaagcag
     5461 gaagggtagg gggtcagcgt tggcgcgctt taaaacgcgg attgcttttg cagatgacgt
     5521 aaactttgcc tttgcgtctg acaatctgac agtcgcggtg gcgttgtttg gcggttttga
     5581 gtgaagacag aacctgcatt atttatcctt tctaaacgat gacatgacgg attggaaacg
     5641 ttggttgaat ttgctggcac ggccttcggt gttgacgttg cgctgcttgc cggtatagac
     5701 aggatgggat gcggaagaag tatccagcga aaacagtgga tattctttgc cgtctgtcca
     5761 aaccatcgtt tttccgtgtg tttcggcgca agaacgaatc agccagcctt cattggcgct
     5821 gctgtcgaaa aaaaggacgg tccggtaatt gtcaggatga atattcggtt tcatatattg
     5881 ccttgcttga agtgttataa cataacaaac tctagcataa tttagaaggg ttgtacaagg
     5941 aaatttaact gttttgtaat atattagaaa ttttcatgat aaacctgaaa attttgaaat
     6001 tgactcatat tcggtgcaat tttattatgt tgcctgaaat atcatataaa agataataaa
     6061 aggtacgcag ccatgaatta cgcaaaagaa atcaatgcgt taaacaacag cctttccgat
     6121 ttgaaaggcg acatcaacgt ttcgtttgaa tttttcccgc cgaaaaacga acaaatggaa
     6181 acgatgctgt gggattccat ccaccgtctg caaaccctgc atcccaagtt cgtatccgta
     6241 acttacggcg caaattccgg cgaacgcgac cgcacgcacg gcatcgtcaa acgcattaag
     6301 caagaaaccg gcttggaagc cgcgccgcac ctgaccggca tcgacgcttc tcccgacgaa
     6361 ttgcgccaaa tcgccaaaga ctattgggac agcggcatcc gccgcattgt cgccctgcgc
     6421 ggcgacgagc cgcccggtta cgagaaaaaa ccgttttatg ccgaagactt ggttaagcta
     6481 ttacgctccg tcgccgactt cgacatctct gtagcagcat atcccgaagt gcatcccgaa
     6541 gcgaaatccg cacaagccga cctgattaat ttgaaacgca aaatcgatgc gggcgcgaac
     6601 cacgtcatca cccaattttt ctttgacgta gaacgctacc tgcgcttccg cgaccgctgc
     6661 gtgatgttgg gtatcgatgt ggaaatcgtc cccggtattt tgcctgttac caacttcaag
     6721 cagctcggta aaatggctca agtaaccaac gtcaaaatcc caagctggct gtcgcaaatg
     6781 tatgaaggtt tggacgacga ccaaggcacg cgcaatctgg tggcggcaag tatcgccatc
     6841 gatatggtca aagtcctgtc ccgcgaaggc gtgaaagatt tccacttcta tacgcttaac
     6901 cgcagcgagc tgacttacgc catttgccat attttaggcg tgcgccctta aagctgaaaa
     6961 cggcttcaga cggcatccga ggccgtttga aaagcaaaac gccgccccat cctagccatt
     7021 ctgatttaca ataccggccg attcggattg aaccggtcct tacaaaatcc aactggagag
     7081 ttcaacatga caacattaca tttctcaggc ttcccgcgtg tcggcgcctt ccgcgaattg
     7141 aaattcgcac aagaaaaata ctggcgcaaa gaaatcagcg agcaagaatt gctggctgtt
     7201 gctaaagact tgcgcgagaa aaactggaaa caccaggccg ctgccaacgc cgatttcgtt
     7261 gccgtaggcg atttcacttt ctacgaccac atcctcgacc tgcaagtcgc caccggcgcg
     7321 attcccgccc gcttcggctt cgacagccaa aacctgtctt tggaacaatt cttccaactg
     7381 gcgcgcggta acaaagacca attcgctatc gaaatgacca aatggttcga caccaactac
     7441 cactacttgg tgcctgaatt ccacgccgat accgaattca aagccaatgc caaacactat
     7501 gttcaacaac tgcaagaagc ccaagccctc ggtctgaaag ccaaaccgac cgttgtaggt
     7561 ccgttgactt tcctgtgggt gggtaaagaa aaaggcgccg tcgaattcga ccgtctgagc
     7621 ctgttgccta aactgttgcc tgtttacgtt gaaatcctga ctgctttggt tgaagccggt
     7681 gccgaatgga ttcaaatcga cgagcctgct ttggctgtcg atttgcctaa agaatgggtg
     7741 gaagcctaca aagacgttta cgctactttg agcaaagtca acgccaaaat cctgttgagc
     7801 acttacttcg gttctgttgc cgaacacgcc gcattgctga aagccctgcc tgttgacggt
     7861 ctgcacatcg acttggtacg cgcacccgag caactggacg cgttcgccga ctacgacaaa
     7921 gttctgtctg ccggcgtgat tgacggccgc aacatttggc gcgccaacct gaacaaagtt
     7981 ttggaaactg ttgagcctct gcaagccaaa ctgggcgacc gtttgtggat ctccagctct
     8041 tgctctctgc tgcacactcc atttgacttg tcagttgaag aaaaactgaa agccaacaaa
     8101 cctgacctgt actcttggtt ggcatttacc ctgcagaaaa cccaagaatt gcgcgttctg
     8161 aaagccgcat tgaacgaagg ccgtgattct gttgccgaag aactggctgc cagccaagct
     8221 gccgccgact cccgtgccaa cagcagcgaa atccaccgtg cagacgttgc caaacgcctg
     8281 gccgatttgc ctgccaacgc agaccaacgc aaatctccat ttgccgaccg tatcaaagcg
     8341 caacaagcat ggttgaacct gcctctgctg ccgacgacca acatcggttc tttcccgcaa
     8401 actaccgaaa tccgccaagc acgcgcagcc ttcaaaaaag gcgaactgtc tgccgccgat
     8461 tacgaagccg cgatgaaaaa agaaatcgcc ttggtggttg aagaacaaga aaaactggat
     8521 ttggacgtac tggtacacgg cgaagccgag cgtaacgaca tggtcgaata cttcggcgaa
     8581 ttgttgagcg gttttgcatt cacccaatac ggctgggtac aaagctacgg ctcacgttgt
     8641 gttaaaccac cgattatctt cggtgacgta agccgtcctg aagccatgac tgtcgcctgg
     8701 tctacttacg cacaaagcct gaccaaacgc ccgatgaaag gtatgttgac cggccctgta
     8761 accattctgc aatggtcttt tgtccgcaac gatattcctc gcgctaccgt gtgcaaacaa
     8821 atcgcactgg ctctgaacga cgaagtattg gatctggaaa aagcaggcat caaagtcatc
     8881 caaattgacg aacctgccat ccgcgaaggc ttgccgctga aacgcgccga ttgggatgcc
     8941 tacctgaact gggcgggcga atccttccgc ctgtcctctg ccggttgcga agacagcacc
     9001 caaatccaca cccatatgtg ttactccgag ttcaacgaca tcttgccggc catcgccgct
     9061 atggatgctg atgtcatcac catcgaaact tcacgttccg atatggaact cttgaccgcg
     9121 ttcggcgaat tcaaataccc gaacgacatc ggcccaggcg tttacgacat ccacagcccg
     9181 cgcgtaccga ctgaagccga agttgagcac ctgttgcgca aagccatcga ggttgtaccg
     9241 gttgagcgtc tgtgggtgaa tcctgactgc ggcctgaaaa cacgtggctg gaaagaaact
     9301 ctggaacaac tccaagtgat gatgaacgta acccgaaaac tgcgtgccga attggcgaaa
     9361 taagccgaga ccgtatgaat aaataccgtc tgaaagcctt tcagacggca ttttgtcctg
     9421 atttgcggcg caagggcgca gttgccggaa aatcttttca ttgcagcttg ttttttctag
     9481 ttcggcttta tatgtggtga acaggcaaat cggagttgtg tttgatagtt ttaaataatt
     9541 tatattattt gaactataaa ttatacaaat cattttgcat ggggtagaat gcccagcgat
     9601 tcacaattat ttctcaaacc aatctattaa ggagcttaaa atggctttgc aagatcgtac
     9661 cggtcaaaaa gtaccttccg tagtattccg cacccgcgtc ggcgacactt ggaaagatgt
     9721 gtctaccgat gatttgttca aaggcaaaaa agtagtcgta ttctccctgc ccggtgcatt
     9781 taccccgact tgttcttctt cacacctgcc gcgttacaac gaattgttcg gcgcgttcaa
     9841 agaaaacggc gttgacgcaa tctactgcgt atctgtaaac gatacgttcg taatgaacgc
     9901 ttgggctgcc gaagaagaat ccgacaacat ctacatgatt cctgacggca acggcgaatt
     9961 taccgaaggt atgggtatgc tggtcggtaa agaagacttg ggcttcggta aacgctcttg
    10021 gcgttactcc atgctggtta acgacggcgt ggttgaaaaa atgttcatcg aacctgaaga
    10081 accgggcgat cctttcaaag tatccgatgc agatactatg ctgcaattcg ttgctcccga
    10141 ttggaaggct caagagtctg ttgccatctt taccaaaccc ggctgccaat tctgcgccaa
    10201 agccaaacaa gctttgcaag acaaaggttt gtcttacgaa gaaatcgtat tgggcaaaga
    10261 tgcaaccgtt acttccgttc gcgccattac cggcaagatg actgctcctc aagtcttcat
    10321 cggcggtaaa tacatcggcg gcagcgaaga tttggaagct tacttggcta aaaactgata
    10381 gctgtttgct taaggcggtt tgattaaact gtctgatata ccggatagag ttattcgggc
    10441 ggttctacac taccgctccg aataactcta tatttataag aggatttgga tattgttgca
    10501 ctcaatcgaa attttgtttt tatttatctg aatgatgttt ttgattggga aaatatttaa
    10561 atgccgtctg aaaccgatat gttctgtgtc ggcaatgttt cagacgaaaa cggaaggaca
    10621 aagattatga aaaaaattca agcggatgtc gtcgtactcg gcggcggtac tgccggtatg
    10681 ggtgcgtttc gcaatgcccg tttacattcg gataatgttt acctgattga aaacaatgtg
    10741 ttcggcacga cctgcgcgcg cgtgggctgt atgccttcca aactcttgat tgccgccgca
    10801 gaggcgcgtc atcacgcatt gcataccgac ccgttcggcg tgcatttgga caaagacagc
    10861 atcgtcgtca acggtgaaga ggtcatgcag cgcgttaaat ccgagcgtga ccgttttgtc
    10921 ggctttgtcg ttgccgatgt ggaagagtgg cctgccgaca agcgcattat gggttcggct
    10981 aaatttatcg acgagcatac cgtccaaatc gacgagcata ctcaaattac ggcaaaaagt
    11041 ttcgtgattg ctaccggttc gcgtcccgtc atcctgccgc aatggcagtc tttgggcaat
    11101 cgtttgatta tcaacgatga cgttttctca tgggatacgc tgcctaagcg cgttgccgtg
    11161 ttcgggccgg gtgttatcgg tttggaactg ggtcaggcat tgcaccgttt gggcgtgaaa
    11221 gttgaaattt tcggtttggg cggaatcatc ggcggcattt ccgaccccgt cgtttcagac
    11281 gaggcgaaag tcgtgttcgg cgaagaattg aaactgcatc tggatgctaa aaccgaggtc
    11341 aaactcgatg cagacggcaa tgtagaagtc cattgggagc aggatggcga aaaaggcgta
    11401 tttgttgccg aatatatgct ggcagccgtg ggccgccgtc cgaacgttga caatatcggt
    11461 ttggaaaata tcaatatcga aaaagatgcg cgcggcgtac ctgttgccga cccgctgacc
    11521 atgcagacca gtattccgca tatcttcatc gcaggcgatg cgtccaacca actgcctctg
    11581 ctgcatgaag ctgccgacca aggcaagatt gccggcgata acgcgggccg ctacccgaat
    11641 atcggcggcg gtttgcgccg cagcaccatc ggcgtggtgt tcaccagtcc gcaaatcggc
    11701 tttgtcggtc tgaaatacgc gcaggttgcc gcgcaatacc aagccgacga atttgtcatc
    11761 ggcgaagtat cgttcaaaaa ccaaggccgc agccgcgtga tgctggtaaa caaaggccat
    11821 atgcgcctgt atgccgaaaa agccaccggc cgcttcatcg gcgcagaaat cgtaggtccg
    11881 gctgccgaac atttggcgca cctgttggct tgggcacatc aaatgaagat gaccgttccg
    11941 caaatgctgg atatgccgtt ctaccatccc gttatcgagg aaggtctgcg taccgcgttg
    12001 cgcgatgccg atgcgaaatt gaaagcctga ccgatatggc aaaacaatgc cgtctgaaat
    12061 tttttcagac ggcattttat ttttggggat ggggtcggat gctgataccg tgtcgggaag
    12121 ggggcggcaa aactaaaaat ctttctttta atctgctgtt tccacgcgtg tttgtcaaaa
    12181 tctatcagtt tgtttttaaa atacactgtt caaaatggga taaaacaggt aaattaacgt
    12241 ttatgtaacc cagtgtagca atgggtttac ggtttttgag tcgatatata actacagagg
    12301 aattgactat gtctgccaaa ccgcgtcctg tttatctgga tttgccgaac atccgtctgc
    12361 cgatacccgg gatagtttcc atccttcacc gcatcagcgg ggtcgggctg tttattatgc
    12421 tgcctttcct gctgtatttc ctgtccggta ccctgagtca agagtctgca tttgaaactt
    12481 accgtgccat tgtttcccat cctttggtca agctggtttt aatcggtgta ttgtgggctt
    12541 atctgcacca ttctctcgcc ggtatccgct ttttattttt ggatgcacac aaaggccttg
    12601 agctgaatac tgcgcgcaat accgctaaag ccgtatttgc ttctgcattg gttttgactg
    12661 tcgttttggg agcgttgtta tggtagaacg taaattgacc ggtgcccatt acggtttgcg
    12721 cgattgggcg atgcaacgtg cgactgcggt tattatgttg atttataccg ttgcactttt
    12781 agtggttcta ttttccctgc ctaaagaata ttcggcatgg caggcatttt ttagtcaaac
    12841 ttgggtaaaa gtatttaccc aagtgagctt catcgccgta ttcttgcacg cttgggtggg
    12901 tatccgcgat ttgtggatgg actatatcaa acccttcggc gtgcgtttgt ttttgcaggt
    12961 tgccaccatc gtttggttgg taggctgctt ggtgtattca gttaaagtaa tttgggggta
    13021 aatatgggtt ttcctgttcg caagtttgat gccgtgattg ttggcggtgg tggtgcaggt
    13081 ttacgcgcag ccctccaatt gtctaaatcc ggcctgaatt gtgctgtttt gtctaaagtg
    13141 ttcccaaccc gttctcatac tgtagcggct caaggtggta tttctgcttc attgggtaat
    13201 gtgcaggaag accgttggga ttggcacatg tacgataccg tcaaaggttc tgactggttg
    13261 ggtgaccaag atgcgattga atttatgtgt cgcgctgcgc ctgaagccgt aattgagttg
    13321 gaacacatgg gtatgccttt cgaccgtgtg gaaagcggta aaatctacca acgtcctttc
    13381 ggtggccata ctgccgaaca cggtaaacgc gcggtagagc gtgcatgtgc ggttgccgac
    13441 cgtacaggtc atgcgatgtt gcatactttg taccaacaaa acgtccgtgc caatacacaa
    13501 ttctttgtgg aatggacagc gcaagatttg attcgtgatg aaaacggcga cgtggtcggt
    13561 gtaaccgcca tggaaatgga aaccggcgaa gtttatattt tccacgctaa agctgtgatg
    13621 tttgctaccg gtggtggtgg tcggatttat gcttcttcta ccaatgctta tatgaatacc
    13681 ggtgacggtt tgggtatttg tgcccgtgca ggtattccgt tggaagacat ggaattctgg
    13741 caattccacc cgaccggtgt ggctggtgcc ggtgtattga ttaccgaggg tgtgcgcggt
    13801 gagggcggta ttctgctaaa tgccgatggc gaacgcttta tggaacgtta cgctccaaca
    13861 gtaaaagatt tggcttcccg tgacgtggtt tcccgtgcga tggcgatgga aatctacgaa
    13921 ggtcgcggtt gtggtaaaaa caaagaccac gtattgctga aaatcgacca tatcggtgca
    13981 gaaaaaatta tggaaaaact gccgggcatc cgcgagattt ccattcagtt tgccggtatc
    14041 gacccgatta aagacccgat tcctgttgtg ccgactaccc actacatgat gggcggtatt
    14101 ccgaccaact accatggcga agttgtcgtt cctcaaggcg acgaatacga agtgcctgta
    14161 aaaggcctgt atgcagcagg tgagtgcgct tgtgcttccg tacacggcgc gaaccgcttg
    14221 ggtactaact ccttgttgga cttggtggta ttcggtaaag ctgccggcga cagtatgatt
    14281 aaattcatca aagagcaaag cgattggaaa cctctgcctg ctaatgccgg tgagttgacc
    14341 cgccaacgta ttgagcgttt ggacaatcaa accggtggcg aaaacgttga tgccttgcgt
    14401 cgtgagctgc aacgctccgt acaactgcac gctggtgtgt tccgtactga tgagattctg
    14461 agcaaaggcg ttcgagaagt catggcgatt gccgagcgtg tgaaacgtac cgaaatcaaa
    14521 gacaagagca aagtgtggaa taccgcgcgt atcgaggctt tggaattgga taacctaatt
    14581 gaagtggcga aagcgacttt ggtgtctgcc gaagcacgta aagaatcacg cggtgcgcac
    14641 gcttcagacg accatcctga gcgcgatgat gaaaactgga tgaaacatac gctgtaccat
    14701 tcagatacca ataccttgtc ctacaaaccg gtgcacacca agcctttgag cgtggaatac
    14761 atcaaaccag ccaagcgcgt ttattgatgc gttttcagac ggtcttcgcc tcaaaggtcg
    14821 tctgaaatct aaccataccc acattgaact gcttgaattt ataatacaaa atcattgggc
    14881 agttgatgag aaaaggaaca cttctcatgg aaaaaatgag ttttgaaatt taccgttaca
    14941 acccggatgt tgatgccaag ccttatatgc agcgttacga gttggaattg gaaccgaccg
    15001 acgtgaaact tttggatgct ttggtacgcc tgaaagcaca agacgatacc ttgtctttcc
    15061 gccgctcctg ccgcgaaggc atttgcggat cggacggtat gaacatcaac ggcaaaaacg
    15121 gcttggcgtg tttgaccgat ctgcgtggct tgaaacagcc agttaaaatc cgtcctctgc
    15181 caggtctgcc tgttatccgc gacctgattg tggatatgac ccagttcttc aaacaatacc
    15241 attccgtcaa accttatgtt gtcaacgata atccgattga tgcggacaaa gagcgtctgc
    15301 aaactcagga agagcgtaaa gagttggacg gtttgtacga gtgtattttg tgcgcctgct
    15361 gttcgactgc ctgcccgtca ttttggtgga accctgataa attcgtcggt ccgtccggtt
    15421 tgctgaatgc ttaccgtttc attgcggaca gccgtgatac catcactaat gagcgtttgg
    15481 ataatctgaa cgacccatac cgtttgttcc gttgccacac cattatgaac tgcgtagacg
    15541 tatgtcctaa acacttgaat ccgacccgag ccatcggtaa gattaaagag attatgttga
    15601 aacgggccgt ttaagaaatg atggtttttg acgatattgc caaacggaaa atccgttttc
    15661 aaacccgccg gggattgttg gaattagatt taatcttcgg caggtttatg gaaaaagaat
    15721 tcgagcattt gagcgataaa gagctgtccg agttttccga aatccttgaa tttcaagatc
    15781 aagaattgct tgccttgatt aacgggcatt cggaaacgga caaagggcac cttatcccga
    15841 tgcttgaaaa aatcagacgg gcatgaggca tctgaaatct gcctgcaggc agatttcaaa
    15901 atgcaaaagc cgtctgaggg caaagaacgt gctgcggatg cagtaacgtg ggttataact
    15961 tgcaaaggag caataatatg tccaaatcaa tcaaactcaa cgtaccgggt caggcaggtt
    16021 tggagctgcc ggtattggaa gccagcatcg ggcacgatgt ggttgacatt cgggggctga
    16081 caaaaaatac aggtttgttt tctttcgacc ccggatttgt ttcaaccgca agctgtgagt
    16141 ctaaaattac ttacatcgac ggcgatcaag gcttgcttta ttatcgcgga taccccatcg
    16201 agcagctggc cgaaaagtcc gattatttgg aagtctgcta cctgttgatt tacggcgaac
    16261 tgccgactcc cgagcaaaag gcagaatttg acaataccgt ccgccgccac acgatggtgc
    16321 atgaacagct gacttggttc ttccgggggt tccgccgcga cgcgcatccg atggcgatga
    16381 tggtcggcgt ggtcggcgca ctgtctgcgt tctaccaaga cagcttggac attagcaatc
    16441 ccgaacaccg caaaatcgcg atttaccgcc tgatttctaa aatcccgacc attgcggcaa
    16501 tgtgctaccg ctattcaaac ggtctgccgt tcaattatcc gaagaataat ctttcttatt
    16561 ccgaaaactt ccttcatatg atgttcgcca cgccgtgtga agactacaaa cccaatcccg
    16621 ttttggcacg cgcgctcgac cgcatcttta ttttgcatgc cgaccacgag caaaacgcct
    16681 caacttcaac cgtccgtttg gcaggctctt cgggtgcgaa tccgtttgcc tgtattgctg
    16741 ccggtatcgc ctgcctgtgg ggtgcttcgc acggcggtgc gaacgaagcc gtgttgaaaa
    16801 tgttggacga aatcggcgat gtgtctaatg ttgccgcata catggaaggt gtgaaacaac
    16861 gcaaataccg tctgatgggc ttcggtcacc gcgtgtaccg caatatggat ccgcgtgcca
    16921 gcattatgcg cgaaacctgc tatgaagttt tgaaggaatt gggcttggaa gacagtccga
    16981 aattcaaact ggcgatggaa ttggaacaga ttgcgctgaa agacccgttc tttatcgaac
    17041 gcaaactgta tccaaacgtc gatttctatt ccggcatcgt cctgtccgcg ctgggcatcc
    17101 cgaccgaaat gtttaccgtc atcttcgccc tgtcgcgcag cgtgggctgg atttcgcact
    17161 ggcacgagat gattagcgat ccttcgctga aaatcggccg cccgcgccag ctttataccg
    17221 gttcggaacg ccgcgattat gtgccgccag gcgagaggta acatacatta atatattgtc
    17281 aaacaggcaa tatcagagaa ccggattgtt tcccgaatcc gtctgattgt agtcggatga
    17341 aatcaagaca agcaatccgg tttaaaatag ggtagaataa aatgtctttt caggcggcat
    17401 cagtttagcc gtcaggacgc ggacttctac cctttgttta tattttaaag aaaagagcgc
    17461 acgccatgat ggacgaaaaa ctcaatttct cttacctgtt cggttcaaac gcaccttaca
    17521 ttgaggaatt gtacgaggct tttttggaaa accccgatgc ggttgatgaa aaatggaagc
    17581 agtatttcac cgatttgagc aaacagccgg ggacggttgc tgtcgatgtc gcacacacac
    17641 cgattcgcga atcatttgtt actttggcga aaaagaaaat tgcatctgcc gttgcgggcg
    17701 gtgcggatga ggcaatgctg aaaaagcaag tcagcgtttt acggctgatt tccgcctatc
    17761 gtatccaagg cgtgggtgca gcccaacttg atccgctcaa acgtatcccc ccgcgcgata
    17821 ttgaagccct cgatccgaaa ttccacggtc tgtcagatgc cgatatggcg cttcaattca
    17881 atatgggcga gggcgatttt gccaatcgcg gcaaactgcc tttgtcccaa atcatcagca
    17941 acctcaaaca aacctactgc ggccacatcg cattggaata tatctatatt cccaataccg
    18001 aagagcgccg ctgggtacgc aattattttg aaagcgtatt gtccacaccg cattacaatg
    18061 ccgatcaaaa acgccgtatc ttgaaagaga tgactgctgc cgaaactttg gaacgttatc
    18121 tgcataccaa atatgtcggt cagaaacgtt tcggtgtcga aggcggcgaa agcgcgattg
    18181 ccggtttgaa ctacctgatt caaaacgccg gtaaagacgg cgtggaagaa gtcatcatcg
    18241 gtatggcgca ccgtggccgt ctgaatgttt tggtgaacat tttgggcaaa aaacccggcg
    18301 atttgtttgc cgaatttgaa ggtcgtgccg aaatcaaact gcccagcggc gacgtgaaat
    18361 accatatggg cttcagctcc gatattgcta ccccgcacgg cccgatgcat gtttctttgg
    18421 cgttcaaccc gtcacacttg gaaatcgtca acccggtggt ggaaggttct gcgcgcgcca
    18481 aacaaaaacg tttgggcgaa aacggccgcg acaaagtctt gccggtattg attcacggcg
    18541 actccgcatt tatcggtctg ggtgtcaacc aagcaacatt caacctgtct aaaacacgcg
    18601 gttataccac cggcggtacg gttcatatcg tcatcaacaa ccaaatcggc tttaccactt
    18661 ccgatatccg cgatacccgt tcaaccgtac actgtaccga tatcgcaaaa atggtttccg
    18721 ctccggttat ccacgtgaac ggcgatgatc ctgagcgcgt ttgctttgcc atccaagccg
    18781 ctttggatta ccgcaaaaaa ttccataaag acatcgtgat tgacgttgtc tgctaccgta
    18841 aatggggtca caacgagggc gatgatccga ccttgaccca accgatgatg tacaaaaaag
    18901 tatcgcaaca ccccggtgcg cgtgctttgt acaccgagca actgattgcc gaaggcgtgg
    18961 taactcaagc agaagctgat gcttacatcc aagcctaccg tgatgctttg gacaaaggcg
    19021 aacatgttga gcaaacaacg ttgagcaatt tccaacgcac acaaatcgat tggggcaaat
    19081 accaaggcaa agattggcgc gaacacatcg aaaccggttt gcctgccgcg gatattgagc
    19141 gtcttaccga gaaatttacc gccgtaccag aaggctttgc cctgcatccg actgcaaaac
    19201 gtgtgattga agcgcgtaaa gccatggcat ccggcaaaca agccatcgac tggggtatgg
    19261 ccgaaacctt ggcatacgca agccttgtaa ccaaaggtca tggcgtgcgt atctccggtg
    19321 aggactccgg tcgcggtacg ttctcgcacc gccatgccgt attgcacgat caaaaccgtg
    19381 aaaaatggga cgacggtact tatgttcctc tgcgccatat gggcgaaggc atgggcgagt
    19441 tcctggttat cgactccatc ttgaacgaag aggccgtgat ggcgttcgag tacggctttg
    19501 cctgctccgc tcctgacaag ctcaccattt gggaagctca attcggtgac ttcgccaacg
    19561 gcgcgcaagt gacgattgac caattcctgt cttcaggcga aaccaaatgg ggtcgcttgt
    19621 gcggtctgac caccatcctg ccgcacggtt acgacggtca aggccctgag cactcttctg
    19681 cacgcgtaga acgttggttg caactgtgtt ctgagaacaa tatgcaagtc atcatgccat
    19741 ctgaagcgtc gcaaatgttc cacctcttgc aacgtcaagt cttaggttca taccgcaaac
    19801 cgctggtgat tttcatgtcc aaacgcctgt tgcgattcaa aggggcaatg agcccgctgg
    19861 aaaacttcac cgaaggttcg actttccgtc cggttatcgg cgataccgcc gaacgcgcaa
    19921 gcaacgacag cgtgaaacgc gtggtattgt gtgccggtca ggtttactat gacttggaag
    19981 ccggtcgagc cgaacgtaaa ctggaagatg atgtcgctat tgtccgcgtt gagcagctgt
    20041 atccgttccc atacgacgag gttaaagccg aactggcgaa atatccgaac gcaaaatctg
    20101 tggtttgggc acaagaagag ccgaaaaacc aaggcgcgtt ctaccaaatc cgccaccgca
    20161 tcgaagacgt tatcagcgaa gagcaaaaac tgtcttatgc cggtcgtcca agcagcgcat
    20221 cgcctgcagt gggctactca agcaaacaca ttgctcaatt gaaacaattg gttgaagacg
    20281 ctttggtatt gtaagccaag tagcattccg tctgaggctg ctcaggcgga acacccatat
    20341 gcagaatgaa aaacacacaa caggccgtct gaaagggcca ttggagacac aaaatgatta
    20401 ttgatgtaaa agtacctatg ctgtctgaaa gcgtatctga aggcacgctc ttggaatgga
    20461 agaaaaaagt tggcgaagca gttgcccgtg acgaaatcct gatcgatatc gaaacggaca
    20521 aagtggtttt ggaagtacct tctccacaag ccggcgtatt ggttgaaatc gtagcgcaag
    20581 acggtgaaac cgttgttgcc gaccaagttt tggcacgtat cgataccgct gctactgccg
    20641 ctgctgaagc acctgccgct gctactgccg ctgctgaagc acctgccgcc gctcctgccg
    20701 aagctgcccc ggccgccgct cctgctgctg cacaaaacaa cgccgctatg cctgccgccg
    20761 ccaaactggc tgcagagagc ggtgttgacg tgaacgcatt gcaaggttcc ggccgtgacg
    20821 gtcgcgtatt gaaagaagac gtacaaaatg ccgcagccaa acctgcccct gccgttgcac
    20881 ttcctgccgg cgcacgtcct gaagaacgcg taccaatgag ccgcctgcgt gcccgtgttg
    20941 cagaacgcct cttggcttct caacaagaaa acgccattct gactacattc aacgaagtca
    21001 acatgaaacc aatcatggac ttgcgtgcga agtacaaaga aaaattcgag aaagaacacg
    21061 gcgtgaaatt gggctttatg tccttcttcg ttaaagccgc tgtcgccgcc ctgaaaaaat
    21121 acccggttgt gaatgcttct gttgacggca aagacatcgt gtaccacggc tacttcgaca
    21181 tcggtatcgc gattggcagc ccacgcggtt tggttgtgcc aatcctgcgc gatgccgacc
    21241 aaatgagcat tgccgacatc gaacaagcaa ttgttgatta cgcgaaaaaa gccaaagacg
    21301 gcaaaatcgc tatcgaagat ctgaccggcg gtacattcag tattaccaac ggcggtactt
    21361 ttggttctat gatgtctacc ccgatcatca acccgcctca atctgcgatt ttgggtatgc
    21421 atgccactaa agagcgcgct gtggttgaaa acggtcaagt tgttgtccgt ccaatgatgt
    21481 atctggctct gtcttacgac caccgtatca tcgacggtcg cgaagctgta ttgaccttgg
    21541 tagccattaa agacgcgttg gaagacccgg cccgcctgtt gttggatctg taatcgtttc
    21601 agacggcctt ttatttgtta atgaaaaggc cgtctgaatt tttatagtgg attaaattta
    21661 aaccagtacg gcgttgcctc gccttgccgt actatctgta ctgcctgcgg cttcgtcgcc
    21721 ttgtcctgat ttaaatttaa tccactatat ttagatgtag cgtaatgtag tatcgtgcta
    21781 caataggctc aacgaacgat tgaggtcgtc tgaaacattt gattcgaatg aatcggcaga
    21841 tatggacttt cagacggctt tttcttaaaa ccatcaaaac gcagtcattc aaaataaaaa
    21901 agaaacgaaa agtatcgttt ttattttgag atactgctaa aagcaaagga tgacacgatg
    21961 tctcaatatg atgtagtagt gattggtgca ggcccgggcg gatacgttgc cgccatccgt
    22021 gccgcacaac tgggtttcaa aactgcctgt gtcgatgcag gcgttaacaa agcaggcaat
    22081 gcccctgcat tgggcggtac ttgcttgaac gtaggttgta tcccttctaa agccctgttg
    22141 caatccagcg aacatttcca cgctgcgcaa cacgattttg ccgaacacgg tatcactgtc
    22201 ggtgacgtaa aattcgacgc ggccaaaatg attgagcgca aagatgccat cgtgaccaaa
    22261 ctgaccggcg gcgtgaaatt cctgttccaa aaaaataaag taaccagtct gttcggtacg
    22321 gcttcctttg ccggtaaaaa tggcgatgct taccaaatcg aagtcgataa caaaggagag
    22381 aaaaccgtta tcgaagccaa acacgtcatc gtagcgaccg gttccgtacc gcgtccgctg
    22441 ccacaagtcg ctatcgacaa tgtgaacgta ttggacaacg aaggcgcatt gaacctgacc
    22501 gaagtacctg ccaaactcgg cgtgatcggt tccggtgtga ttggtttgga aatgggttcc
    22561 gtatggaacc gcgtgggtgc ggaagttacc attcttgaag ccgcgccgac cttcctggct
    22621 gccgccgacc aacaaatcgc caaagaagcc ttcaaatact tcaccaaaga gcaaggtctg
    22681 agcatcgaat tgggcgtgaa aatcggcgac atcaagtctg aaggcaaagg tgtttccgtt
    22741 gcttacgaaa ctgctgctgg cgaagccaaa accgaagtat tcgacaaact gatcgttgcc
    22801 atcggccgta ttccaaacac caaaggcctg aacgcggaag ccgtaggctt ggaaaaagac
    22861 gagcgcggct ttatcaaagt agatggcgaa tgccgtacca acctgcctaa cgtatgggca
    22921 atcggcgacg tggttcgcgg cccgatgttg gcacacaaag ccagcgacga aggcgttgcc
    22981 gttgccgaac gcattgccgg tcaaaaaccg catatcgact tcaacaacgt accgttcgtg
    23041 atttacaccg atcctgaaat cgcttgggtg ggtaaaaccg aagagcagct caaagccgaa
    23101 ggcgtggagt acaaaaaagg tacttcaggt tttggtgcga atggtcgcgc attggcaatg
    23161 ggcaaagcca aaggtacggt taaagtgttg gcagatgcca aaaccgaccg catcttgggc
    23221 gtacacatga ttggtccggt tgtcagcgaa ttggttaccg aaggcgtgac tgcgctcgaa
    23281 ttcttcgcca gcagcgaaga catcgcccgc attatccatg cccacccaac cttgtccgaa
    23341 gtggttcacg aagctgcatt ggcggccgac aaacgcgctt tgcacggttg atagacatta
    23401 aggccgtctg aaatttttca gacggcctta aggccttcga caaattgaat gttccgagag
    23461 ctccgttttc tgatttataa ttccgtcaga caaacaaaca gcatttacat tcattatgaa
    23521 caaagaaata gtcggtattt tctttatacc ggcgggcatc atcagcatgt gtatggccgc
    23581 attgtggcag atgtatgtga tgatgaccga aacttatacg ctcaaccgtt tcaaagataa
    23641 agaattggtt tggcgcgtgg cattgttgtt tatcagtttc agccttgccg tttatctgct
    23701 ctgtccgaat tcgcgtaaaa aaggcatcgt cttttttatt ctcgggggag gcggtgcagc
    23761 catgtatctg ctggcgcgga tgtggttgcc tttcagcaag tgaaacgacg attttccgac
    23821 cgccgaaagg tagtctgaaa cgcacgggct tgccatttgg aggcagactc ggggcattcc
    23881 actaatctaa aggagaaatc catgaattta cacgagtatc aggctaaaga actgctggct
    23941 agctacggtt tgcccgtaca aggcggtatt ttggcacaca acggcgaaga agccgctgca
    24001 gcttacgaca aattgggcgg caaattcgct gttgtcaaag cacaagtaca cgccggcggc
    24061 cgcggtaaag cgggcggcgt aaaagtcgtt aaaagccgcg aagaagctaa agaagtggct
    24121 gaaagcctga ttggcaccaa cttggtaact taccaaaccg atgccaacgg ccaacctgtc
    24181 aacagtgttt tggtttgcga agacatgtat ccggttcaaa ccgagctgta cttgggcgca
    24241 gtggttgacc gttctacccg ccgcattaca ttcatggcct ctaccgaagg cggcgtggaa
    24301 atcgaaaaag ttgctgccga aactcctgaa aaaatcttca aagtaaccgt tgatccgctg
    24361 gtcggcctgc aaccttgcca agctcgcgaa gttgcgttcc aactgggctt gaaagacaaa
    24421 caaatcaacg agttcgtcaa actgatgacc ggtgcgtaca aagcgtttgt cgaaaatgac
    24481 ttcgccctgt ttgaagtcaa cccgctggca gttcgcgaaa acggcgcgct cgcctgcgtg
    24541 gacggcaaaa tcggcatcga cagcaacgcg ctctaccgcc tgccgaaaat cgccgaattg
    24601 cgcgacaaat ctcaagaaaa cgaacgcgag ttgaaagctt ctgaatttga cctgaactat
    24661 gttgccctgg aaggcaacat cggctgtatg gtgaacggtg ccggtttggc gatggccact
    24721 atggacatca tcaaactgaa aggcggccaa cctgccaact tcttggacgt tggcggcggc
    24781 gcaaccaaag accgcgtggt tgaagcgttc aaactgattc tggaagacaa atccgttcaa
    24841 ggcgtattga tcaacatctt cggcggtatc gtacgttgcg acatgattgc ggaagccatc
    24901 gtggcagccg ttaaagaaat caacgtcaac gttcctgtcg ttgttcgttt ggaaggcaac
    24961 aacgccgaac tcggcgcgaa aatcctgaac gaatcaggtc tgaaactgac ttctgcagac
    25021 ggcctgaatg acgcagccga aaaaattgtt gcagccgtaa acgcctaagg agaaaagaat
    25081 gagcgtattg attaataaag acactaaagt attggttcaa ggtttcaccg gtaaaaacgg
    25141 tactttccac tccgaacaag ctctggctta cggcactaaa gttgtcggcg gcgttacccc
    25201 gggcaaaggc ggtcaaaccc acctgaacct gcccgtgttc aacaccatga aagaagccgt
    25261 taaagaaacc ggcgcggatg catccgtgat ttacgttcct gctccgtttg tgttggattc
    25321 tatcgttgaa gcagttgatt caggcgtagg cttggtcgtt gtgattaccg aaggcgtgcc
    25381 gactttggac atgctcaaag ccaaacgcta cttggaaacc aacggtaacg gaacacgttt
    25441 ggtcggccct aactgcccgg gcgtgattac tccgggcgag tgcaaaatcg gcattatgcc
    25501 gggccacatc catactcccg gccgcatcgg catcatttcc cgttccggta cattgactta
    25561 cgaagccgtg gcacaaacca ccaaactggg cttgggtcaa tcaacctgta tcggtatcgg
    25621 cggcgacccg attccgggta tgaaccaaat cgacgcactg aaacttttcc aagaagaccc
    25681 ggataccgac gccatcatca tgatcggtga aattggcggt actgcggaag aagaagcagc
    25741 cgaatacatc caatccaacg taagcaaacc tgttgtcggc tatatcgccg gtgttaccgc
    25801 acctaaaggc aaacgcatgg gtcacgccgg tgcgattatc tccggcggca aaggtactgc
    25861 ggaagaaaaa ttcgccgctt tcgaaaaagc cggtatcgct tacacccgca gccctgccga
    25921 gttgggcact accatgctgg aagtgttgaa agcaaaaggt ttggcataat caggtttgac
    25981 aactgattga acatcaaatg ccgtctgaaa ccggaaatcg ggtttcagac ggcattttgt
    26041 ttgtcatttc aaaaagaggc agcctcaaca tacccacatt atttttgccc ttttggggca
    26101 gtcagagacc tttgcaaaat tccccaaaat cccctaaatt cccaccaaga catttagggg
    26161 attttgggga attttgcaaa ggtctcgggc taagtgtgcc tgtttgcgcc taaaaggcgg
    26221 cccggatgcc tgattatcgg gtatcctggg aggattaagg gggtattggg gtaaaattag
    26281 tggatatttg aaacgaaaac agccgaaaac ctgtgtttgg gtttcggctg tcgggaggga
    26341 aaggaatttt gcaaagctct cgtattggct ttgaagttcc gtgtaattca caggtagggc
    26401 gtgtggcaca gccacgcacg cggtcggttg ggtatgcagg ctacggcttt ctctgttgaa
    26461 actgcaacac gtcagctacc aaccgtgtgc gtagcgcaca cggttggttg ggatacaagt
    26521 tactgtttgt tcttaaatgg agtaccaaca tcaagggctt ttgataatcc tgaaaatatt
    26581 aaatattcag tttcagtttt tattttagga gaaatatttg cataatttct atctttaaag
    26641 caccaatgga tatatggttt cgtttcatct tctgggttat ctaaataagc aataacattc
    26701 aagtcaaata aaaattgcaa aaactcatta gcagtactca taaatttagg tatttccact
    26761 gatgttgttt gtaagtgctt tttcaaacgt tcaaatgctt ttaaaaaatc actatattta
    26821 aatctatctt tcccgtttaa aaattcaaaa aatttcagga aattttgata atcactttga
    26881 ctataataaa acaaaagatg atctttgatt tcaccaagta aatatatcga gtattctctt
    26941 tgaaaagaag tattatcaaa atcttctgct acgacataat cttccttact tttcttattt
    27001 ttttgtagca aagtaagcat ctgaagaata tcgcgaggtc gataatacga ttttcttagg
    27061 aagctaataa atgaagttaa atttttatac tcatcatgta aattaggagc attccatgga
    27121 aaataataat cccatgagtt gcctttttct aaactatctt gtttttcttg ctgggttctc
    27181 aaaagatgat caaaaacgcc aaaaatcttt gaacttctat aagatttata atccgtcctc
    27241 cagtctaaaa atactgaatt atcttgaagt ttggtatttt gattttgtaa acctaatgaa
    27301 tcaaagatat caggtctaat caataacaca actctcatcc ttcccttact atctttaatg
    27361 gaagggaaga tatcattatt taacatccat atggcgttag caagaccttt tacacactca
    27421 tgatattcat caaatggaat ctgtgatggt ctaatatcta tcccatcaat aaacaaaata
    27481 tgattatctt ttagctttaa ctgagataaa gcatctttaa attttctttc aataaaacct
    27541 aaatttgctt ggaatttact ttctgtaaaa gttatttgtt gggattcctc ttcacctagt
    27601 ttaacaaatt ttccaaaaat catttccgca gcttcttttg aattttctat taaagttatt
    27661 gcttgtacaa tttccggatc aaaagcgcca taataatatt catttatagc ctcatctaag
    27721 gctttaaatt tattaaatat tgaagataat attccgtttt ctttacattt gatttgattt
    27781 gatatcaaca gatataaaat gactttccaa atacttgtaa aatctgaaac agttaagtgt
    27841 cttgctttct ttagctgaat aaattttgaa taatcggttt cacgaacaaa cttagtagtg
    27901 gcatgtatgt ttttatagaa gttattagtt aaataaacag catatgctgt ctttccagtt
    27961 cccttttctc cgattaaaaa cgaaatattt ggttcacata attcatccaa atattctcct
    28021 tttacaaata ttcggttaaa taaatcttta ttttctcttc ttctgtagtt tgcagcatcc
    28081 acaaatccaa attctaatgt ttttaacggt ttcatcttaa taatctccta tttaattttg
    28141 aattaaactt acctcaaaac caccttcaaa tacttcccag tataactccc cttaactttc
    28201 gccacctgct cagggctacc acaagcaata atcctgcccc cgccatcgcc gccttccggc
    28261 cctaagtcca caatccaatc ggcggtttta atcacgtcta gattatgctc gataatcaca
    28321 atcgagttgc ctttgccttt cagacgacct atgacttcca gcagcagggc gatgtcggcg
    28381 aagtgtaggc cggtggtggg ttcgtcgagg atgtagagtg ttctgccggt gtcgcgtttg
    28441 gagagttcca aggcgagttt gacgcgctgg gcttcgccgc cggagagggt ggtggcggat
    28501 tggccgaggc ggatgtagcc gagacctacg tccatcaggg tttgcagttt gcgcgatacg
    28561 gtggggacgg cgtcgaaaaa ttcgcgggct tcttcgacgg tcatgtcgag gacttggctg
    28621 atgtttttgc ctttgtattg gatttcgagc gtttcgcggt tgtagcgttt gccgtggcag
    28681 acttcgcagg ggacgtacac gtcgggcagg aagtgcattt cgactttaat cacgccgtcg
    28741 ccttggcagg cttcgcagcg gccgcctttg acgttgaagg agaatctgcc gacgttgtag
    28801 cctcgttcac gcgagagggg tacgctggcg aagagttcgc ggatgggggt gaacaggccg
    28861 gtgtaggtgg cgggattgga gcgtggggtg cggccgatgg gggattggtc gacgttgatg
    28921 actttgtcga ggtgttcgag gccgcggatg tcgtcgtatg gggcgggttc ttcttgggcg
    28981 cggttgagtt cgcgggcggt aattttggcg agggtgtcgt taatcagggt ggatttgccg
    29041 ctgccggata cgccggtgat gcaggtaatc aaaccgagcg gcagctcaag ggtaacgttt
    29101 ttgagattgt tgccgcgtgc gcctttgagg acgagcatcc ggtcgggatt gacgggcgtg
    29161 cgttcagacg gcacggcaat ggattttttg ccgctgaggt attgtccggt aacggagttt
    29221 tcgcattggg cgacgttttc gggcgtgtcg gcaatcagta cgttgccgcc gtgttcgcct
    29281 gcgccggggc ccatatcgac cacgaaatcg gcttcgcgga tggcgtcttc gtcgtgttcg
    29341 accacaatca cgctgttgcc caaatcgcgc agtcgtttga gggtggccag caggcggtca
    29401 ttgtcacgct ggtgcaggcc gatggagggt tcgtccagta catacatcac gccggtcagg
    29461 ccgctgccga tttggctggc gaggcggatg cgctgggctt cgccgccgga gagggtttcg
    29521 gcggagcggc ttaaattcag gtaatccagc ccgacgttaa tcaggaagcc gaggcgttcg
    29581 gtgatttctt tgaggatttt ttcggcgatt tgttttttgt tgccgtctaa atccagtgtt
    29641 tcaaagaatt ggtgggtttt ggtgagcggc caggcggaaa cttcgtgcag cggttcgccg
    29701 ctgacgtaaa cgtagcgggc ttctttgcgc aaacgtgcac caccgcagct cgggcaggcg
    29761 cggtggttct ggtattcgcg caatttttcg cgcacggttt cgctgtcggt ttcgcggtag
    29821 cggcgttcga gattgggaat gatgccttcg aaggcgtggc tgcggttgaa ggtggtgccg
    29881 cgttcggata ggtaggtgaa atcaatgact tccttgcccg agccgtgcag cacgactttt
    29941 ttgacttttt caggcagcgt ttcccaagca gcctgcacat cgaaaccgta atgccgcgcc
    30001 agcgactgaa tcatttggaa atagaactgg ttgcgcttat cccagccgtc aatcgcgccc
    30061 gttgccagcg acaattcggg atgcgcgacc actttttcgg gatcgaagaa gttggtgttg
    30121 cccaagccgt cgcaagttgg gcaggagccc atcgggttgt tgaacgaaaa caggcgcggc
    30181 tctaattcgg gcaggctgta tgaacatacg ggacaggcga aacgcgcgga aaaccaatgt
    30241 tcttcgccgc tttccatttc catcgccagc gcgcgctcgt tgccgtggcg cagtgcggtt
    30301 tcaaaacttt ccgccagccg ctgcttgatg tccgccttca ctttcacgcg gtcgatgacc
    30361 acgtcgatat tgtgcttgat gtttttctcc agcttcggca cttcgtcaag ctgataaact
    30421 tcgccgtcca cgcgcacacg ggcaaagcct tgcgcctgca agtcggcaaa gaagtcgaca
    30481 aactcgccct tacgctcacg aactgccggc gcaagaatca tcacgcgcgt gtcttccggc
    30541 agttttaaga cggcatcgac catttgcgac acggtctggc tcgacaacgg caaattgtgt
    30601 tcggggcaat acggcgtacc gacgcgggcg tacaaaaggc gcaggtaatc atggatttcc
    30661 gttaccgtac cgacggtgga gcgcggattg tggctggtgg atttctgctc gatggaaatc
    30721 gcgggcgaca gaccctcgat caaatcgaca tcgggtttgt ccatcatctg caaaaactgc
    30781 cgcgcatagg cggacaggct ctcgacataa cgccgctgtc cttcggcata cagcgtgtcg
    30841 aacgccagcg acgatttgcc gctgcccgac aatcctgtta ccaccaccag cttgtggcgc
    30901 ggaatgtcca aatcgatgtt tttcaaatta tgcgtgcgcg cgccgcgtat gcggatggtg
    30961 tcgttgtcgt gcgaatgttg gggatgatac ttgcacataa tggatgccgc ctgaaaaata
    31021 aaggaaaact ggtattgtag cactttctcg gatgccgtct gaagccgcgt tcagacggca
    31081 tttgtcggcg gagtacggca gattccgcta taatgtcggc aattttaacc cgcttgaaca
    31141 aaaggatgac aaatgaaccg tctttacccc cacccgatta tcgcccgtga gggctggccg
    31201 attattggcg gcggtttggc tttgagcctg ctggtgtcga tgtgttgcgg ctggtggtct
    31261 ttgccgtttt gggtgtttac cgtatttgcc ctgcagtttt tccgcgaccc tgcgcgtgag
    31321 attccgcaaa atcctgaagc agtgttgagt ccggttgacg gccgtatcgt ggtggtcgag
    31381 cgcgcacgcg atccgtatcg tgatgtcgat gctttgaaaa tcagtatttt tatgaacgtg
    31441 ttcaacgtgc attcgcaaaa atcgcctgcc gattgtacgg taacgaaagt ggtctataac
    31501 aaaggcaaat tcgtgaatgc ggatttggac aaagccagca cggaaaacga acgtaatgcg
    31561 gtgttggcga ctacggcttc aggtcgtgaa attacttttg ttcaagtggc cggtttggtg
    31621 gcgcgccgta ttttgtgcta cacccaagca ggtgcgaaac tgtctcgcgg cgaacgctat
    31681 ggctttatcc gcttcggttc gcgcgtggat atgtatctgc ctgtcgatgc gcaggcgcaa
    31741 gtggcgattg gcgataaagt taccggcgtc agcactgtat tggcgcgttt gccgctgact
    31801 gcgccgcaaa tcgaatccga gcctgaatct gagcctgctt tacaagctgc tccggttgaa
    31861 acagcggcaa acccatctgc cgaacaacgg caaatcgagg cagcggcggc taagattcaa
    31921 gcggctgtgc aagatgtgtt gaaagattaa ttttgcgaac taaaatagaa aatatcagta
    31981 ccatcattca cacgaatgag gaagtttggt tttttgaatt tttgctaatg ttcacaccgt
    32041 cattcccacg aaagtgggaa tctagaaact taacgttacg acgatttatc ggaaacgact
    32101 gaaaccggac ggactggatt cccgcctgcg cgggaatgac ggcacattag ttacctaaaa
    32161 cttaaaaaaa aaacagaaac ctttccgcct cattcccacg aaagtgggaa tcta
//
LOCUS       DAXHKX010000005        28853 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHKX010000005 DAXHKX010000000
VERSION     DAXHKX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.005"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            80..1045
                     /locus_tag="ACFMWZ_000237"
     CDS             80..1045
                     /locus_tag="ACFMWZ_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC5934262.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQSESASL
                     TVKTAHGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1209..2177
                     /locus_tag="ACFMWZ_000238"
     CDS             1209..2177
                     /locus_tag="ACFMWZ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC5934263.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGASQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDILGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2167..3141
                     /locus_tag="ACFMWZ_000239"
     CDS             2167..3141
                     /locus_tag="ACFMWZ_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC5934264.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AINLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3186..3812
                     /locus_tag="ACFMWZ_000240"
     CDS             3186..3812
                     /locus_tag="ACFMWZ_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC5934265.1"
                     /translation="MTQERFPSFFNQAPTITVRDALAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYPMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPE
                     MRELMPKAVGGSASAEELERFRQLWQARVKAFLTESADDPQFVIVREV"
     gene            3895..4653
                     /locus_tag="ACFMWZ_000241"
     CDS             3895..4653
                     /locus_tag="ACFMWZ_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC5934266.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLL
                     SFMARLQPLAHGNISYAGKDTKTTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4712..5050)
                     /locus_tag="ACFMWZ_000242"
     CDS             complement(4712..5050)
                     /locus_tag="ACFMWZ_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC5934267.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5201..9163
                     /gene="purL"
                     /locus_tag="ACFMWZ_000243"
     CDS             5201..9163
                     /gene="purL"
                     /locus_tag="ACFMWZ_000243"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC5934268.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLTDIDAAAQLFHHIQSET
                     FSSVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAINIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            9258..10016
                     /gene="gloB"
                     /locus_tag="ACFMWZ_000244"
     CDS             9258..10016
                     /gene="gloB"
                     /locus_tag="ACFMWZ_000244"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC5934269.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNQLPEST
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10323..14795
                     /locus_tag="ACFMWZ_000245"
                     /pseudo
     CDS             10323..14795
                     /locus_tag="ACFMWZ_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
     gene            15027..16481
                     /gene="mgtE"
                     /locus_tag="ACFMWZ_000246"
     CDS             15027..16481
                     /gene="mgtE"
                     /locus_tag="ACFMWZ_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC5934270.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(16567..17475)
                     /gene="hslO"
                     /locus_tag="ACFMWZ_000247"
     CDS             complement(16567..17475)
                     /gene="hslO"
                     /locus_tag="ACFMWZ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC5934271.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENEWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            17720..18475
                     /locus_tag="ACFMWZ_000248"
     CDS             17720..18475
                     /locus_tag="ACFMWZ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC5934272.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            18494..18712
                     /locus_tag="ACFMWZ_000249"
     CDS             18494..18712
                     /locus_tag="ACFMWZ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934273.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            18857..19201
                     /locus_tag="ACFMWZ_000250"
     CDS             18857..19201
                     /locus_tag="ACFMWZ_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC5934274.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            19201..19893
                     /locus_tag="ACFMWZ_000251"
     CDS             19201..19893
                     /locus_tag="ACFMWZ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC5934275.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            19960..21180
                     /gene="argJ"
                     /locus_tag="ACFMWZ_000252"
     CDS             19960..21180
                     /gene="argJ"
                     /locus_tag="ACFMWZ_000252"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC5934276.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            21343..22752
                     /locus_tag="ACFMWZ_000253"
     CDS             21343..22752
                     /locus_tag="ACFMWZ_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC5934277.1"
                     /translation="MTSTFPHRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            22942..24333
                     /gene="hrpA"
                     /locus_tag="ACFMWZ_000254"
     CDS             22942..24333
                     /gene="hrpA"
                     /locus_tag="ACFMWZ_000254"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC5934278.1"
                     /translation="MSPNFAQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKAIAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            24434..25561
                     /locus_tag="ACFMWZ_000255"
     CDS             24434..25561
                     /locus_tag="ACFMWZ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC5934279.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQCPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQGTQVLRIDGTKIVYIK"
     gene            25738..27315
                     /gene="lpt3"
                     /locus_tag="ACFMWZ_000256"
     CDS             25738..27315
                     /gene="lpt3"
                     /locus_tag="ACFMWZ_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC5934280.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPNKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            27417..>28853
                     /locus_tag="ACFMWZ_000257"
     CDS             27417..>28853
                     /locus_tag="ACFMWZ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC5934281.1"
                     /translation="MDARSNPANVSDDLQNSSGHIGVNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMCEWRELHHQLAQTAIEMGLTTKETA
                     FRRPPEIRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDETLFAFYHERLP
                     NFYTADAVSDGLRPANPQQTAPSYAREERREGKTVAAQTNFSATAANPLPNPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGR"
BASE COUNT         6988 a         8820 c         7205 g         5840 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccta tgttacgttt gactgcttta gccgtatgca ccgccctcgc
      121 tttgggcgcg tgttcgccgc aaaattccga ctctgcccca caagccaaag aacaggcggt
      181 ttccgccgca caatccgaaa gtgcgtccct gaccgtcaaa acggcgcacg gcgatgttca
      241 aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg acaccttgag
      301 caaactgggc gtgaaaaccg gtttgtccgt cgataaaaac cgcctgccgt atttagagga
      361 atatttcaaa acgaccaaac ccgccggaac tttgttcgag ccggattacg aaaccttaaa
      421 tgcctacaag ccgcagctca tcatcatcgg cagccgcgcc gccaaggcgt ttgacaaatt
      481 aaacgaaatc gcgccgacca tcgaaatgac cgccgatacc gccaacctca aagaaagtgc
      541 caaagagcgt atcgacgcgc tggcgcaaat cttcggcaaa caggcggaag ccgacaagct
      601 gaaggcggaa atcgatgctt cgtttgaagc ggcgaaaact gccgcacaag gcaaaggcaa
      661 aggtttggtg attttggtca acggcggcaa gatgtccgcc ttcggcccgt cttcacgact
      721 gggcggctgg ctgcacaaag acatcggttt gcccgctgtt gacgaagcca tcaaagaagg
      781 cagccacggt cagcctatca gctttgaata cctgaaagag aaaaatcccg actggctgtt
      841 tgtcctcgac cgaagcgcgg ccatcggcga agagggtcag gcggcgaaag acgtgttgga
      901 taatccgctg gttgccgaaa caaccgcttg gaaaaaagga caagtcgttt accttgttcc
      961 tgaaacttat ttggcggccg gtggcgcgca agagctgctg aatgcaagca aacaggttgc
     1021 cgacgctttt aacgccgcga aataatgaaa cggcggcatt cgatgccgtc tgaaacacgg
     1081 atgcaaaccg cttcctgtgt ttcagacggc attgcccgat acggaggctt caaacaaggc
     1141 tttccgctcc gacggttcgg actgccttgt ttgaatcttc tacgccgtaa cgcttttccc
     1201 ttctgtttat gactgccaaa cctttttccc tcaacctgac taacctcctg ctgctggcgg
     1261 tgttgtttgc cgtcagcctg tcggtcggcg ttgccgattt ccgctggtcg gatgtgtttt
     1321 cgctgtccga cagccagcag gttatgttca tcagccgcct gccgcgcacg tttgcgattg
     1381 tgctgacggg cgcgtcgatg gcggtggcgg ggatgattat gcagattctg atgcgcaacc
     1441 gttttgtcga accttctatg gcgggcgcga gccaaagtgc ggctttgggt ttgcttctga
     1501 tgtccctgct gctgcctgcc gcgccgctgc cggtcaaaat gtcggttgcc gccgttgccg
     1561 cgctaatcgg aatgctggtg tttatgatgc ttatccgccg cctgccgccg acggcgcaac
     1621 tgatggtgcc gctggtgggg attattttcg gcggcgtggt tgaggcggtg gcgaccttta
     1681 tcgcgtatga aaacgaaatg ctgcaaatgc tcggcgtgtg gcagcagggc gatttttcga
     1741 gcgtgctgct ggggcggtac gagctgcttt ggattacggg cggtttggcg gtgtttgcct
     1801 atctgattgc cgaccggctg acgattttgg ggctgggcga gacggtcagc gtgaatttgg
     1861 gtttgaaccg gacggcggtg ttgtggtcgg ggctgattat tgtggctttg attacgtcgc
     1921 tggttatcgt tacggtcggc aatattccgt ttatcgggct ggtcgtgccg aacatcatca
     1981 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtggctttg ttgggcgcgt
     2041 ctttggtttt attgtgcgat attttgggac gcgtgattgt gtttccgttt gaaattccgg
     2101 tctcgaccgt cttcggggta ttgggtacgg cgttgttttt gtggctttta ttaaggaaac
     2161 ctgcccatgc cgtctgaaaa aaatatcggt tttatggcag gaagcagccg tccgttgtgg
     2221 gtagcttttg cattgttgct ggtttcctgc gtcctgttta tgacgctcaa cgtcaaaggc
     2281 gattgggatt ttgtactgca cctgcgtctg accaaacttg ccgcgctgct gatggtcgcc
     2341 tatgcggtcg gcgtgtccac gcagcttttc caaacgctga ccaacaatcc gattctgacc
     2401 ccttcgattt taggttttga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2461 ttcggcggcg tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaactggtt
     2521 gttatgatgg gcggctcgct gctgctgttt tacacgctca tcaaacaggg cgggcgcgat
     2581 ttgccgcgta tgattttaat cggcgtgatt tttgggattt tgttccgcag tctgtcgtcg
     2641 ctgctttcgc gcatgataga ccccgaagaa tttacggcgg cgcaggcgaa tatgtttgcc
     2701 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc
     2761 gcggcggtcg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2821 gccatcaatt tgggcatcag ctacacgcgc aacaccttat ggatactgct ttggattgcc
     2881 gcattggtgg cgacggcgac cgccgttgtc ggcccggtaa gctttttcgg gcttctcgcc
     2941 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gccgatgacg
     3001 gtttgtgtcg gcggcattct cttggtcggc ggacagaccg tattcgaaca cttcttgggc
     3061 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     3121 gttttaaaac acaaaaaatg acggatgccg tctgaacggc cgcccgcctc gaaaggacaa
     3181 accatatgac gcaagaacgt ttcccatcat tcttcaacca agccccgacc atcaccgtca
     3241 gggacgcgct tgccgcattt ctcggcgcgg cgcaagacgg catcctcact taccgctacg
     3301 ccgatgccgt gcgcctgtgc ggacattcct gcccgaccgt cgcgggcgcg tacccgatgg
     3361 ttatcaaagg tctgaaagcc ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3421 ccgccatgca gggaacgcgc gacgaaggca cggtcggcgt aaccgcgtcc gtcgtccaac
     3481 tcctcaccgg cgcagccccc gaaaccggct tcggaggcat cggaatgcag ggacgcttcg
     3541 cccgccgcaa cctcttatcc tttggtgcgg gcgaaatcaa cggcacactg accctgcgcc
     3601 gcaaagacaa cggcaaaacc gtcgccgtca gcctcaacgc cgccctgcaa cccttcgcac
     3661 ccgaaatgcg cgaacttatg cccaaagccg tcggcggcag cgcaagcgca gaagaactcg
     3721 aacgcttcag acaactctgg caggcgcgcg ttaaagcatt tttgaccgaa tcggcggacg
     3781 acccgcagtt cgtcatcgtc cgcgaagtat gagcgttcag acggcatttt gaatttcaaa
     3841 tgccgtctga accccgccaa acaacaacaa acctacgccc gacaagcatc cgccatgatt
     3901 accatccgca acgtcagcta ccgcatcggc acacgcccca tcctcgacaa cgtcagcctc
     3961 gacattcccg aaggcggcat taccgcgctc gtcggcccga acggcgcagg caaatccacc
     4021 ctcctctcct ttatggcgcg gcttcagcct ctggcacacg gcaacatcag ctacgcaggc
     4081 aaagacacca aaaccacccc caccgccgaa cttgcccgca cgctctccat cctcacccaa
     4141 gaaaacagca tcatgagccg catcaccgtg cgcgacctgc tgatgttcgg ccgctatccc
     4201 taccaccaag gcaggccgac tgccgaatgc cgccgtatcg ttaacggtgc aatcgaagaa
     4261 ttccacctgc aagacctctc cgaccgctat ctgaccgaac tctccggcgg acaacgccaa
     4321 cgcgccatga ttgcgatggt gttctgccaa agcaccgact acgtcctttt ggacgaaccg
     4381 ctgaacaacc tcgatatgta tcacgcccgc tcgctcatgc agattctgca aaagctcacg
     4441 cgcgaacaca aacgcaccac cgtcgccgta ttgcacgaca ttaaccaggc cgccgcttac
     4501 gccgaccacg tcgtcgccat gaaaaacggc aaagccgtcc tgaccggcac gcccgaagaa
     4561 gtgttcaccg tacacaacat caaagaactg ttcgacatgg acgtggacgt actcgactac
     4621 gaaggcaaaa aactcatcgt ccaccatatc tgaagctcaa aaagaaaatg ccgtctgaaa
     4681 ggctttcaga cggcatttcc attccaaacg ctcaaaccgc cgcgtccgca cgttcgcccg
     4741 tgcggatacg gattgcctcc tcgaccggca gcacaaaaat cttgccgtcg ccgatttttc
     4801 ccgaacgcgc cacctcgaca atcacgtcaa tcgcgcgttc cacagcatca tccgccaaca
     4861 ccagctcgat tttgaccttg ggcaggaaat cgacagcgta ttccgcgccc cggtaaatct
     4921 ccgtatgccc cttctgcctg ccgaaccctt tgacctcgct gacggtcata cccgtaatgc
     4981 cgatttccgt caacgcctcg cgcacgtcgt cgagtttgaa cggtttgaca atcgcctcga
     5041 tttttttcat aaaatttcct ttgaacaaac aatacaaaca catccgaaaa acgggaacct
     5101 cccgtcagat tgtcaacatt ttaagccaaa atacccaagc aatacagccc cgttgcgcgt
     5161 ataatgacag attttccaac cgcatttgag agccgaatcc atgtctgtcg ttttgccctt
     5221 gcgcggcgtt accgcccttt ccgatttccg tgtcgaaaaa ctcttgcaaa aagccgccgc
     5281 actcggactg cccgaagtca aattaagcag cgaattttgg tatttcgccg gcagcgagaa
     5341 agcattggat gccgcgactg tcgaaaaact gcaagccttg ttggcggcgc aaagcgttga
     5401 acaaacgcca aaagcgcgcg agggcttgca tttgtttttg gtcacgcccc gtttgggtac
     5461 gatttcgccg tgggcttcca aggcgaccaa tatcgcggaa aactgcggtt tggcaggcat
     5521 cgaacgcatc gagcgcggca tggcggtgtg gctggaaggt gcgcttaccg acaggcaaaa
     5581 acaacaatgg gcagccctgt tgcacgaccg catgaccgaa agcgtgttga ccgacatcga
     5641 tgcggcggcg caactgttcc accacatcca atccgaaact ttctccagcg tcgatgtttt
     5701 gggcggcggt aaagaggctt tggtcaaagc caataccgaa atgggcttgg cactctccgc
     5761 cgacgaaatc gattatctgg ttgaaaacta tcaggctttg cagcgcaatc cgtccgatgt
     5821 tgaattgatg atgttcgcgc aggcaaacag cgaacactgc cgccacaaaa tcttcaacgc
     5881 cgatttcatc ctcaacggcg aaaaacaacc gaaatccctg ttcggcatga tacgcgacac
     5941 gcacaacgcg catcccgaag gtacggtcgt cgcttataaa gacaattcgt ccgtaatcga
     6001 aggcgcgaaa atcgagcgtt tctatccgga tgcggcggaa aaccaaggct accgtttcca
     6061 cgaggaagac acgcatatca tcatgaaagt ggaaacgcac aaccacccga ccgccatcgc
     6121 gccgtttgcg ggcgcggcga cgggcgcggg cggcgaaatc cgcgacgaag gtgcgacggg
     6181 caaaggttcg cgtccgaaag cgggcttgac cggctttacc gtgtccaacc tcaacatccc
     6241 cggcctcaaa cagccgtggg aacaagatta cggcaagccg gaacatattt cctcgccgct
     6301 ggacattatg attgaaggtc ccatcggcgg cgcggcgttc aacaacgaat tcggccgccc
     6361 caacctcttg ggctacttcc gcacctttga agaaaaattt gacggtcagg ttcgcggcta
     6421 ccacaaaccg attatgattg ccggcggtct gggcagcatt caggcgcagc agacgcataa
     6481 agacgaaatc cccgaaggcg cattgctgat tcaactgggc ggcccgggca tgcttatcgg
     6541 cttgggcggc ggcgcggctt cttcgatgga taccggcacg aacgacgcgt ctttagactt
     6601 caattccgta caacgcggca accccgaaat cgaacgccgc gcgcaggaag tcatcgaccg
     6661 ctgctggcag ctcggcggca aaaacccgat tatctccatc cacgacgtgg gcgcgggcgg
     6721 tttgtccaac gccttccccg aactcgtcaa cgatgccgga cgcggcgcgg tattcaagct
     6781 gcgcgaagtg ccgttggaag aacacggttt aacgcctttg caaatctggt gtaacgaatc
     6841 gcaagagcgt tatgtgttgt cgattttgga aaaagatttg gacaccttcc gcgccatctg
     6901 cgaacgcgaa cgctgcccgt ttgccgtggt cggcacggcg accgacgacg gccatttgaa
     6961 agtgcgcgac gatttgttcg ccaacaaccc cgtcgatttg ccgctgaacg tcttgctcgg
     7021 caaaccgccc aaaaccacgc gtaccgacaa aacggttacg ccgtccaaaa aaccgtttca
     7081 cgccgacgat atcgacatca ccgaagccgc ctaccgcgtt ttgcgcctgc ctgccgtagc
     7141 cgccaaaaac ttcctgatta ccatcggcga ccgcagcgtc ggcggcatga cccaccgcga
     7201 ccaaatggtc ggcaaatacc aaacccccgt agccgactgc gccgttacca tgatgggctt
     7261 caatacctat cgcggcgaag caatgtctat gggcgaaaaa ccgaccgttg ccctgtttga
     7321 cgcgcccgcc tcgggcagaa tgtgcgtcgg cgaagccatc accaacatcg cggcgatcaa
     7381 catcggcgac atcggcaata tcaaactctc cgccaactgg atggcggcgt gcggcaacga
     7441 aggcgaagac gaaaaactct accgcaccgt cgaagcggtt tccaaagcct gtcaggcatt
     7501 ggatttgagc attcccgtgg gcaaagacag cctgtcgatg aaaaccgtat ggcaggatgg
     7561 cgaagagaaa aaatccgtcg tttcgccgtt aagcctgatt atctccgcgt tcgcgccggt
     7621 tcaagacgta cgcaaaaccg ttacgcccga attgaaaaac gtcgaggaca gcgtattgct
     7681 gtttgtcgat ttgggcttcg gcaaagcgcg tatgggcggc tctgcattcg gtcaggtata
     7741 caacaacatg agcggcgacg cgcccgattt ggacgacaca ggccgtctga aagcgtttta
     7801 caacgtgatt caacagcttg tcgccgaaga caaactcttg gcataccacg accgcagcga
     7861 cggcggcttg tttgccgttt tggtagaaat ggcgtttgca gggcggtgcg gcttggatat
     7921 agatttaaat ttattgcttg cacaaacatt tattaccaac cataccgctc tgtctcaatc
     7981 attgcggact gaagaggtaa aagcgttggc tgaatggcaa gaaaccattg cccgcacatt
     8041 atttaatgaa gagttgggtg ctgttatcca agttagaaaa caagatgttg ccgatattat
     8101 caatttattc tatcaacaac agctgcatca taatgtcttt gaaatcggta cgttaactga
     8161 tgagaacacg ttaatcatcc gcgacgggca aacgcacctt atttctgaca acctaatcaa
     8221 actgcaacaa acctggcaag aaaccagcca tcaaatccaa cgcctgcgcg acaaccctgc
     8281 ctgcgccgac agcgagtttg ccctgattgg cgacaacgac cgcagcgcat tgtttgccaa
     8341 cctgaaattc gacgtgaacg aagacatcgc cgcgccgttt atcaacagcg gcgcgaaacc
     8401 caaaatcgcc atcctgcgcg aacagggcgt gaacgggcaa atcgaaatgg ccgccgcgtt
     8461 cacccgtgcc ggattcgatg cctacgacgt gcatatgtcc gacctgatgg caggccgctt
     8521 ccgccttgcc gacttcaaaa tgctggcggc gtgcggcggc ttcagctacg gcgacgtact
     8581 cggcgcgggc gaaggctggg cgaaatccat cctgttccac cctgctctgc gcgaccagtt
     8641 tgccgccttc ttcgccgacc cggacacgct gacattgggc gtgtgcaacg gctgccaaat
     8701 ggtcagcaac ctcgccgaga ttatcccggg tgcagaggca tggccgaagt tcaaacgtaa
     8761 cttgagcgaa cagttcgaag cgcgcctgag tatggttcac gtccccaaat ccgcgtcgct
     8821 gattctgaac gaaatgcaag gctccagcct gcccgtcgtc gtcagccacg gcgaaggccg
     8881 cgccgacttc gcgcttcacg gcggcaacat ttctgctgat ttgggcatcg cgctgcaata
     8941 tgtggacgga caaaaccagg ttacccaaac ctatccgctc aaccctaacg gctcgccgca
     9001 aggcatcgcc ggtgtgacca acgccgacgg ccgcgtgacc attatgatgc cccaccccga
     9061 acgcgtgtac cgtgccgcgc aaatgagctg gaaaccggaa ggctggacgg aactgtccgg
     9121 ctggtaccgc ctctttgccg gcgcacgtaa agccttgggc taaccgccct actcaaacca
     9181 atgccgcctg aagaatattt cagacggcgt tccggcatac catcctttaa acggtatccg
     9241 tccaccgagg aacactcatg aaaatcaccc ccgtcaaagc cttgaccgac aactacatct
     9301 ggatgataca gcacggcaac catgccgtct gcgtcgaccc ttccgaaccc tcgcccgtct
     9361 tggaattcct cgtccgcaac cgcctcatgc ttgcccaaac atgggtaact cacccccatc
     9421 ccgaccacga gggcggtgcg gcggcactct ggcgcggcta catggaatcg cccgtttacg
     9481 gcgaatccga catcgaagca gcaacccaca ccgtaaccgc cggcacccaa ttcaccttcg
     9541 gcgacggaca ggttaccgtt tgggcaacac ccggccacac agaccgccac accagctacc
     9601 ttctcgaaac ttcagacggc atacacgttt tctgcggcga caccctgttt tccgccggct
     9661 gcggacgcgt gtttaccggc acaatcgaac agctttacga cagcttccaa cgcttcaacc
     9721 aattacctga aagcaccctg ttctacccgg cacacgaata caccgccgcc aacctggatt
     9781 ttgccgccca tatcgagccg gacaacgccg acattcagac ggcactgaaa gcggcggcgc
     9841 atacgcctac cctgcccgtt accctcgcgc acgaacgccg tgtcaatccg tttttgcgcg
     9901 tcgacctgcc gcacgtcaga gaccgcgccg aggcattgag cgggaaaacg ttaaacagca
     9961 gcctcgatac ctttgtcgcg ctgcgtgaac ttaaaaacca ataccggacg aaataaaaca
    10021 acgggaaaac gcagccattc ctaggatttt tattaaaatc ttaaataaaa tcatacaatc
    10081 atcgccaata gacgaaagga caccgttgcc ttataatcaa acaaaaacaa aatatataat
    10141 atagtggatt gaatttaaat caggacaagg cgacgaagcc gcagacaata cagatagtac
    10201 ggcaaggcga ggcaacgctg tactggtttt tgttaatcca ctatataaat ccagcacaaa
    10261 acgggatcgg tgattcttgt ccgcaagaat cgttgatttt ctctattaca cggataatca
    10321 tcatgcgctt cacacacacc accccatttt gttccgtatt gtccaccctc ggtctttttg
    10381 ccgtttcccc tgcttactca tccattgtcc gcaacgatgt cgattaccaa tattttcgcg
    10441 actttgccga aaataaaggc gcgttcgccg taggtgcaag caatatttcc atccaagaca
    10501 agcaaggcaa aatattaggc agggttctca acggcatccc catgcccgac ttccgagtca
    10561 gcaaccggca aaccgccatc gccaccctga ttcaccccca atacgtcaac agtgtcaaac
    10621 acaacgtcgg ctacggttcc atacaattcg gcaacgacac ccaaaatcca gaagaacaag
    10681 cctataccta ccgcctcgta tcacgcaacc cgcacccgga ctacgactac caccttcccc
    10741 gcctcaacaa actggttacc gaaatctcac ctgccgcact cagcagcgta cccttgcttg
    10801 gaaacggcca gccaaaggcc aatgcctacc tcgataccga ccgcttcccc tactttgtac
    10861 gactcggctc aggcacgcaa caagtccgca aagcagacgg cacgcgtaca cgaaccgccc
    10921 cggcatacca atacctgacc ggcggcacgc cgctgaaagt attggggttc caaaaccacg
    10981 gcttactcgt cggcgacagc ctgaccgacc aaccccttaa cacctacgca atcgccggag
    11041 acagcggttc cccctgtttg ccttcgacaa gcatgaaaac cgctgggtgc ttgcgggcgt
    11101 actcagcacc tacgccggct tcgataattt cttcaacaaa tacatcgtca cgcaacccga
    11161 attcatccgt tccaccatcc gccaatacga aacccggctg gatgtcgggc tgaccaccaa
    11221 cgaactcata tggcgcgaca acggtaatgg cgacagcacc ctgcaagggc tcaacgaacg
    11281 catcaccctg cccattgcaa acccttcgct tgccccacaa aacgacagca ggcacatgcc
    11341 gtctgaagat gccggcaaaa cgctcatcct atccagcagg ttcgacaaca aaacactgat
    11401 gctggcagac aatatcaacc aaggcgcagg cgcattgcag ttcgacagca acttcaccgt
    11461 cgtcggtaaa aaccacacat ggcaaggtgc aggcgttatc gtagccgacg gcaaacgcgt
    11521 cttctggcaa gtcagcaacc ccaaaggcga ccggctctcc aaactgggcg caggcacgct
    11581 tatcgccaac ggacaaggca tcaaccaggg cgacatcagc atcggggaag gcactgtcgt
    11641 actcgcccaa aaagctgctt cagacggcag caaacaagca ttcaaccaag tcggcatcac
    11701 cagcggcagg ggcacggccg tcctcgccga cagccagcaa atcaaatccg aaaacctcta
    11761 tttcggcttc aggggcggac ggctcgacct caacggcaac aaccttgcct ttacccatat
    11821 ccgccatgcg gacggcggcg cgcaaatcgt caatcacaac cctgaccaag ccgcgacact
    11881 gacgctgacc ggcaaccccg tcctcagtcc cgagcatgtc gagtgggtgc aatggggcaa
    11941 ccgtccgcaa ggcaacgcgg cggtttacga atacatcaac ccgcaccgca accgtcggac
    12001 cgactacttc atactcaaac ccggcggcaa cccgcgcgaa tttttcccgt taaatatgga
    12061 aaactcaaca agctggcaat ttgtcggcaa caacaggcaa caggccgccg aacaagtcgc
    12121 ccaagccgaa aatgcccgcc ccgacctgat taccttcggc ggatacttgg gtgagaacgc
    12181 gcaaacgggt aaagccgcac cgagttacag caaaaccaat atcgaatggg tgaatagtcc
    12241 aaacacccca cacaagccct cgccctatac gaatacgcca acccgcacag gggcaggcgt
    12301 accgacgatt accgcctcaa agaaggcggc aaccccggcg cctacttccc atgggatgcc
    12361 tacagcaccc aagactggga atttctcgga cataaccggc gaagcagcca tagaaaaaaa
    12421 ccgccatatc gcaaatgccg ccgtatacgg ccggcccgaa taccgttaca acggcgcact
    12481 caacctgcac tatcgtccca aacgcaccga cagcacgctg ttgctcaacg gcggcatgaa
    12541 ccttaacggg gaagtcttga ttgagggcgg caatatgatt gtgtcaggca ggcccgtacc
    12601 ccatgcctac gaccaccagg ccaaacgcga acccgttctt gaaaacgaat ggaccgacgg
    12661 cagcttcaag gctgcacggt tcaccctacg aaaccatgcc cgactgacag cagggcgcaa
    12721 taccgcgcat ctggacggcg acataaccgc atacaatctg tccggcatcg acctcggctt
    12781 tatccaaggc aaaacaccgg aatgctaccg ctcctaccat agcggcagca cccactgcac
    12841 acccaacgcc gttttaaaag ccgaaaacta tcgtgcacta cctgtaacgc aagtacgcgg
    12901 cgacattacc cttaacgacc gttcagagct ccgcctgggc aaagcacacc tgtacggcag
    12961 catccgtgcc ggcaaagaca ccgcagtccg catggaagca gacagcaact ggacactttc
    13021 ccagtccagc cacaccggcg cactgacgct tgacggcgca caaattaccc tgaaccccga
    13081 tttcgccaat aatacacaca acaaccgctt caacacactg accgtcaacg gcacacttga
    13141 cgggttcggc acattccgat tcctgaccgg catcgtccga aaacaaaatg ccccccctca
    13201 aactggaagg ggacagccgc ggcgcattcc aaatccacgt caaaaacacc ggacaagaac
    13261 ctcaaacaac cgaatcgctt gcacttgtga gcctcaatcc gaaacacagc caccaagccc
    13321 gatttaccct ccaaaacggc tatgccgatt tgggtgccta ccgctacatc ctccgcaaaa
    13381 acaacaacgg atacagcctg tacaacccgc tcaaagaggc cgaacttcaa attgaagcca
    13441 cgcgtgcgga acatgagcgc aaccaacagg catacaacca attacaggca accgacatca
    13501 gcagacaggt tcaacatgac tctgacgcga ccaggcaggc actacaggcc tggcagaaca
    13561 gtcaaaccga acttgcccgc atcgacagcc aagtccaata tctgtccgcc caattgaaac
    13621 agacagaccc gctgaccggc attctgacgc gtgcccaaaa cctgtgtgcc gcacaaggat
    13681 acagtgccga tatctgccgt caggttgcca aagccgccga cacgaacgac ctgacactct
    13741 tcgaaaccga actggatacg tatatagaac gtgtagaaat ggccgaatcc gaacttgaca
    13801 aagcacggca aggcggcgat gcgcaagccg tcgaaacagc ccggcacgcc tacctgaacg
    13861 cactcaaccg tctgtcccga caaatccaca gtttgaaaac cggcgttgcc ggcatccgta
    13921 tgccgaacct ggccgaactg atcagccggt cggccaacac cgccgtttcc gaacaggccg
    13981 cctacaatac cggccggcaa caggcgggac gccgcatcga ccgccacctt accgatccgc
    14041 agcagcaaaa catctggctg gaaaccggta cgcaacaaac cgactaccat agcggcacac
    14101 accgtcccta ccaacaaact accaactatg cacatatcgg catccaaacc ggcatcaccg
    14161 accgtctcag tgtcggtaca attttaaccg atgagcgcac aaacaaccgt tttgatgaag
    14221 gcgtatccgc ccgaaaccgc agcaacggcg cacatctgtt cgtcaaaggg gaaaacggcg
    14281 cactctttgc cgcggcagat ttaggctaca gcaacagccg tacccgattt accgattatg
    14341 acggggctgc cgtccgccgc cacgcatggg atgcaggcat caacaccggc atcaaaatcg
    14401 ataccggcat caacctcaga ccctatgccg gcatccgtat aaaccgcagc aacggcaacc
    14461 ggtacgtact cgacggcgca gagataaaca gcccggcgca aatccaaacc acatggcatg
    14521 ccggcatccg tctcgataaa accgtcgaac tgggtcaagc caagctgacc cccgctttca
    14581 gcagcgatta ctaccatacc cgccaaaaca gcggttccgc cctcagcgtc aacgaccgta
    14641 ccttactgca gcaagccgcc cacggcacac tgcataccct gcaaatcgac gccggataca
    14701 aaggctggaa cgccaaactt catgccgctt acggcaaaga cagcaacacc gcccgccaca
    14761 aacaggcagg aatcaaaata ggctacaact ggtaacaagc cgataaaaat gccgtctgga
    14821 acccgcgttt cagacggcat ttgcgttaaa aatagtaaac cgttccaaaa gggagtagaa
    14881 tagtgccgtt tccaaccctg cgcctgtgcc gtcaggcttt tattatggac cttcccagtt
    14941 cgtttttact gaacacccct tccgattcca aacgacaata accatcccgc cggaatgcct
    15001 ccccgcgcac ggcgggcgga gcatttatga gcatcgaacc aacccctccg aaccttgaaa
    15061 acgacggtat cgaaaacgat gtagaacgcg tttccgccga tttcgaccgt gtccactccc
    15121 tctgcgaaat cctcgaacct gcctttgaac aaatcgaaaa cggcacaccg ctcgaagacg
    15181 cgccgctgcg cgacaagctg accgagctga ccgtcctctt gagcgagctg caccctgccg
    15241 acgtggcggc agtgttggaa tcgctgccgc cgcgcgaacg caatatcgtc tggattctgg
    15301 tcaaaccgga agacgacggc gaagtcttgc tggaagtgtc cgatgcggtg cgcgaaacgc
    15361 tgatcgagtc gatggacaaa gacgaattgt tggcggcggt cgatgatttg gacgcggacg
    15421 aattggcgga gctggcagac gatttgccgc accaagtggt ttatgaagcc ttgcagacgc
    15481 gcgacgagga agagcgcgca caagtcaaag cggcaatgtc ctacgaagac aaccaagtcg
    15541 gtgcgattat ggacttcgag ttggtcagca tccgcgccga tgtcgcctgt gaagtggtgc
    15601 tgcgctatct gcgccgcttc gacagcctgc ccgaccatac cgacaagatt tttgtggtcg
    15661 atgaaaacga cgtactgcaa ggcgtgctgc ccatccgcaa acttttggtc gccgatcccg
    15721 aagatttggt ggaaaacgtg atggcgaaag atgtcgtgcg tttccgcgcc gaagacgacg
    15781 tggaagaagc agcgcaggca ttcgaacgct atgacttggt taccgcgccc gtcgtcgatg
    15841 aaaacaaaaa gctcatcggc agggtgaccg ttgacgaaat ggtggacgtg atccgcgaag
    15901 aatccgaagc ggatatgttg aacatggcag gtttgcagga agaggaagac ctgttcgccc
    15961 ccgtcttgga ttccgtcaaa aaccgctgga tgtggctcgc cgtcaacctc tgcaccgcct
    16021 tcctcgccag ccgcgtcatc ggcgcgtttg aaggcagcat cgaaaaaatc gtcgcactcg
    16081 ccgcgctgat gcccatcgtc gccggcatcg gcggcaactc gggcaaccag acgattacca
    16141 tgattgtccg cgcgatggcg atggggcagc tgacggatat gcaggcgggg cgtttgctga
    16201 aaaaagaagt cggtgtcgcc ttggtcaacg gcatcatttg gggaacggtc atgggcgcag
    16261 tatcttggct gctttacggc agcctcggca tcgggctggt tatgattgcc gcgatgacgc
    16321 tcaacctcct gctggcggca accgtcggcg tattaattcc cgtggtaatg gaaaagttcg
    16381 gacgcgatcc cgcactgggc agctcggtgc tgattaccgc cgttaccgac tccggcggct
    16441 tcctgatttt cttggggctc gccaccctat tcctgcttta aatgccgtct gaacccgcgc
    16501 aaaaatgccg tctgaagcgg aagctgcttc agacggcatt tgactattta tccttgttgc
    16561 acaagattat tggacggtat gccggggcag ccctttggca acgccgacca catcctcccc
    16621 gaacagcgcg ttgacatcgg tttcgtcaaa cacatatttg ctgtggcaga aatcgcaatc
    16681 gacttcgatg ctgccttgtt ccaccaccac gccgccgact tcttccccgc ccagcatcaa
    16741 cagcatatcg ctgactttgc cgcgcgaaca ggtgcatgaa aattcaaacg tttccggctc
    16801 gaacacgcgc ggcggcgttt cgtggaacaa gcggtacaaa acatgttgcg catccaattc
    16861 tgccaactcg cccgccgtca gcgtgcgcgc cagcgtactg acgtgttccc atgcctcttc
    16921 atccaacacc tcttcaggca gacgctgcac cagcagaccg cccgccgctt catcgcttgc
    16981 agacaggacg atgtgcgtat caagctgttc ggaacgtttc atatagttca tcaacatttg
    17041 cgcgataccg tcgccttcca aaggcactac gccctgccag ggttcgccgt ctttgggctg
    17101 cagcgtcagc acgaatacgc cgccctcgcc caaaagatcg accaaacttt catcttcacc
    17161 gatttctgcc gcctcgtccc accgcgcggt cgcacggacg gtacggtcgg aagtcgcttc
    17221 cgcaaccagc attttcagcc gcccctgccc ctgaacctgc acaatcagcg tgccttcgtt
    17281 tttgaggttg cccgacagca gaacaccggc agccaacagc tcgcccaagg cgcggcggat
    17341 ggcggcggga tagtttttct gttttacaat gtgctgccac tcgttttcca gacggacgtg
    17401 cagcccgcgc acgggcatat cgtcgaagat aaagcgggta cgcacatcgg cgcggttgat
    17461 ggcggtttga ttcatgattt tctctgactg attgttcgga tggcggctat atggttgcgg
    17521 tcggcgcgaa aacaagacgg acggcggatg cgcttcccaa attatcaata aattatataa
    17581 aaatcaacat attaactcaa tctaacaagc cgttttttgc caaacagccg tttttttata
    17641 tacaatcaac aagatatttt cgactgatac agcataacat cgcacggcgg cacgatgcct
    17701 cctgcgcgga aacaccgata tggattcttt tttcaaaccg gcagtttggg cggttttgtg
    17761 gctgatgttt gccgtccgcc ccgcccttgc cgacgagttg accaacctgc tcagcagccg
    17821 cgagcagatt ctcagacagt ttgccgaaga cgaacagccc gttttaccca tcaaccgagc
    17881 ccccgcccgg cgggcgggca atgccgacga actcatcggc agcgcgatgg ggcttaacga
    17941 acagcccgtt ttacccgtca accgagtccc cgcccggcgg gcgggcaatg ccgacgaact
    18001 catcggcaac gcgatggggc ttaacgaaca gcccgtttta cccgtcaacc gagcccccgc
    18061 ccggcgggcg ggcaatgccg acgaactcat cggcaacgcg atgggacttt tgggtattgc
    18121 ctaccgctac ggcggcacat cgatttctac cggttttgac tgcagcggct tcatgcagca
    18181 catcttcaaa cgcgccatgg gcatcaacct gccgcgcacg tcggcagaac aggcgcggat
    18241 gggtacgccg gttgcccgaa gcgaattgca gcccggggat atggtgtttt tccgcacgct
    18301 cggcggcagc cgcatttccc atgtcggact ttatatcggc aacaaccgct tcatccacgc
    18361 gccgcgcacg gggaaaaata tcgaaatcac cagcctgagc cacaaatatt ggagcggcaa
    18421 atacgcgttc gcccgccggg tcaagaaaaa cgacccgtcc cgctttctga actgatttcc
    18481 caaggaatac gcaatgagta tgcccgaaat gcccaaatgg tacgacgatg acggacagat
    18541 cgtgtcctgt accgaaaagg tcaaagtgat gtccgaaaat atggccgagc tgtatcagac
    18601 ggcacaagac gcgtttgaag acgcgctgct gatgggttgc ggcgaacgtc agttgcgcga
    18661 ttacctgctc gcgctgattg aaggtttgga aaatccctac cgcaaagtct gaacacgccc
    18721 cggttgctgc ggcacggttt atccgtgccg tttttgcgtt tgtgcgcggc ttcggctttt
    18781 cagacggcat atttgacgtt atgattaaac agttaacaag atttatcaca acgccgtcaa
    18841 aagacagaca cacaacatga acatcatccg cgcgctcctc atcatcctcg gctgcctcgc
    18901 cgccggcgaa accgccgttt tcctagcagg catcaaactg cccggcagca tcgtcggcat
    18961 gggcgtgctg tttgcgcttt tgcaggcagg ttgggtcaaa acgtcttggc tgcaacagct
    19021 taccgacgcg ctgatggcga atctgacgct gtttctcgtg ccgccctgcg tggcggtcat
    19081 cagctatttg gatttgattg ccgacgattg gttttcgata ctggtttccg cctccgccag
    19141 cactttgtgc gtactgctgg ttacaggcaa ggttcaccgc tggatacgga gcattatctg
    19201 atgaacgaaa tcctcaggca gcccagcgtt ctgcttttcc tcacgcttgc cgtgtacgcg
    19261 cttgcgatta tcgtgcgcac gcgcacgggc aatatcttct gcaaccccgt actcgtcagc
    19321 actatcgtgc tgattgccta cctgaaaatc ctcgacatcg attatgcggt gtaccacaac
    19381 gccgcgcaat tcattgattt ttggctgaaa cccgccgtcg tcgtgcttgc cgtgccgctc
    19441 taccaaaacc gccgtaaaat cttcaaccag tggctgcccg tcatcgtttc acagcttgcg
    19501 ggcagcgtta cgggcattgt tacggggatg tattttgcca aatggctggg cgcggaacgc
    19561 gaagtcgtcc tctcgctcgc gtccaaatct gttaccaacc ccatcgccat tgaaatcacc
    19621 cgctccatcg gcggcattcc cgccattacc gccgccaccg tcatcattgc cggtctggtc
    19681 ggacagattg ccggttacaa aatgctgaag aacacagtcg tcatgccctc gtccgtgggt
    19741 atgtcgctcg gcacggcttc gcacgcgatg gggattgccg cctcgctcga acgcagccgc
    19801 cgcatggcgg catacgcggg actggggctg acgttcaacg gcgtactgac cgcgctgatt
    19861 gcgccgctgc tcatccccgt tttgggattt tgaacccgtt tcagacggca tttcagccca
    19921 tgctgtctga acgccgacac actcgcaagg agaaccgtta tggctgtcaa cctgactgaa
    19981 aaaaccgccg aacaactgcc cgacatcgac ggcattgccc tctacaccgc ccaagcaggc
    20041 gtgaagaagc ccgggcatac cgacctgaca ctgattgccg tagccgccgg cagcaccgtc
    20101 ggtgcagtct tcacgaccaa ccgtttctgt gccgcgcccg tccacatcgc caaatcgcac
    20161 cttttcgacg aagacggcgt gcgcgccctc atcatcaaca cgggcaacgc caacgcgggt
    20221 acgggcgcac agggcagaat cgatgctttg gcagtgtgtg ccgccgccgc ccggcaaatc
    20281 ggctgcaaac cgaaccaggt gctgcccttc tccaccggcg tgattctcga accgctgccc
    20341 gcagacaaaa tcatcgccgc gctgcccaaa atgcagcctg ccttctggaa cgaagcggca
    20401 cgcgccatca tgaccaccga caccgtgccc aaagccgcct cgcgcgaagg caaggtcggc
    20461 gacaaacaca ccgtccgcgc cacgggcatc gccaaaggct cgggcatgat tcatcccaat
    20521 atggcgacca tgctcggttt catcgccacc gatgccaaag tttcccaacc cgtcctccaa
    20581 ctgatgacgc aggaaatcgc cgacgaaacc ttcaacacca tcaccgttga cggcgacacc
    20641 agcaccaacg acagcttcgt catcatcgcc accggcaaaa acagccaaag cgaaatcgac
    20701 aacatcgccg acccgcgtta cgcccaactc aaagaattgt tgtgcagcct cgcgctcgaa
    20761 ctcgcccaag ccatcgtccg cgacggcgaa ggtgcgacca agttcatcac cgtccgcgtc
    20821 gaaaacgcca aaacccgcga cgaagcccgc caagccgcct acgccgtggc acgttcgccg
    20881 ctggtcaaaa ccgccttttt cgcctccgac cccaacctcg gcaggctgct cgccgccatc
    20941 ggttatgccg gcgttgccga cctcgatacc gacctcgtgg aaatgtatct cgacgatatt
    21001 ttggttgccg aacacggcgg acgcgccgca agctacaccg aagcacaagg gcaggcggta
    21061 atgtcgaagg ccgaaatcac cgtccgcatc aagctgcatc gcggacaagc cgccgccacc
    21121 gtctatacct gcgacctgtc gcacggatac gtttccatca acgccgacta ccgttcctga
    21181 cccgacacgg cttcagacgg catacatata gtggattaac aaaaatcagg acaaggcgac
    21241 gaagccgcag acagtacaaa tagtacggca agacgaggca acgctgtact ggtttttgtt
    21301 aatccactat aaaatgccgt ctgaaccgcc ggacaacata ccatgacctc cacattcccc
    21361 caccgcctcg cccgcaaaat ccgccaaacc cgccgcctgt cgcgcaaaag catcgccttt
    21421 ctgttccttt tggcaggttc ggcactcgtc gccctgaccg cgctgttttt tgcccatctt
    21481 gccgattttg cgctggaact gaacgccaaa ctggttcaac aatacccgtg gttcgcgtgg
    21541 gtcgcgcttc ctttgggttt gccgcttatt gcgtggctca cacgcaaatt cgcccccttc
    21601 accgccggca gcggcatccc gcaggtcatc gcctcactgt cgctgcccta cggcgcacag
    21661 aaaacgcggc tgatccgcct cgggcagacg ctgctgaaga ttccgctgac ctttttgggt
    21721 atgctgttcg gcgcgtccat cggacgcgaa ggtccgtccg tgcaggtcgg cgcggcagtg
    21781 atgggcgcgt ggggcgcgtg gtgcaagaaa cacggcttgg cattcaaagg gatgcaggaa
    21841 aacgatttga tggcggcggg cgcggcgggc ggtttggcag ccgcgttcaa cgcgccgctg
    21901 gcgggcgtga ttttcgccat tgaggaactc gggcgcggca tcatgttgcg ctgggagagg
    21961 caaattcttt tgggcgtgct cgcctccggt ttcatacagg tcgccattca gggcaacaac
    22021 ccgtattttt ccggcttcaa cggcggcgta ttggaacata tctttctgtg ggtcgcactg
    22081 tccggcctgg tttgcggcgc ggcgggcggg ctgttcggac gtttgctcta tcgcggtgcg
    22141 gcggcgtttg caccgcgcaa gatacgcggc ttcatccgca accgtccgct gctgctggcg
    22201 gcactgatgg ggctgctgct cgccctgctc ggcacgttct accaaggcaa aacctacggc
    22261 accggctacc acgaagccgc ccaagccctg cacggcatct acgaagcccc cttcggactc
    22321 gccgccgcca aatggctcgc caccgtattc agctattggg caggcattcc gggcggcatt
    22381 ttcactccct cgctgaccat aggcgcggtt ttgggcgagc atatcgccgc catcgccgac
    22441 atatcgcagg gtgcaaacat catcgtcctc atctgcatgg cggcatttct ggcgggcgcg
    22501 acacaatccc cgattacttc cgccgtcgtc gtcatggaaa tgacgggcgg acaaagcctg
    22561 ctgttttgga tgctaattgc ctgcattttc gcctcgcagg tttcgcgcca gttttcgcca
    22621 cgtccgttct accacgcatc gggaatgcgc ttccgccagc gcgtgcttca agaaaccgcc
    22681 gcccaaaccg gcaatgcgcc cgcaagaccg caaacagcaa acagcaaaac gggaatgccg
    22741 tctgaaaatt aaaacgcccc cgatcaaacg ccggcagccg ccttgatttg aataccgttc
    22801 cgccgccgct tgaaacttca gcaacaatgc cgtctgaacg acagaatgcg gttttcagac
    22861 ggcatttccc catcccgata ttgcccgaac ccaaccaaag cgtttgctat aatttcattt
    22921 ttaccgtacc cgcacacaaa tatgtccccc aatttcgccc aaaccctctc caaagaccgc
    22981 cacttcctgc aatccgcctt caaaaatccc aacaaatacg gcggcttgtc caaagtcgag
    23041 gaaaaatacc gaaaatcgca cgaaatcttt ttgaaacgtt tggccgcctt gcccaaaccc
    23101 gaattcgaca acaccctgcc cgttcacgag aaacttgacg aaatcaaaaa agccattgcc
    23161 gagcatcagg taacgattat ttgcggcgaa accggttcgg gcaaaaccac gcagctgccc
    23221 aagatttgtt tagaactcgg gcgtggggcg gcggggctga tcgggcatac ccagccgcgc
    23281 cgtttggccg cgcgctccgt ggcagagcgg attgccgaag agctgaaatc cgaaatcggc
    23341 agcgcggtcg gctataaagt acgcttcacc gaccacacct cgcgcgatgc ctgcgtcaag
    23401 ctgatgaccg acggcatcct gctggcggaa acgcagaccg accgttatct cgccgcctac
    23461 gacacgatta tcattgacga agcgcacgag cgcagcctga acatcgactt cctcttgggc
    23521 tatttgaaac aactgctgcc gcgccgcccc gatttgaaag tcatcatcac ctcggcaacc
    23581 atagacgcag aacgcttctc ccaacacttc aacggcgcgc ccgtgctgga agtgagcgga
    23641 cgcacctatc ccgtcgaaat cctctaccga ccgctgaccg gcaaagacga agacgatgca
    23701 gaagtcgagc tgaccgacgc gattgtcgat gcagccgacg aattggcgcg acacggcgaa
    23761 ggcgatatct tggtattcct gccgggcgag cgcgaaatcc gcgaagccgc cgaagccctg
    23821 cgcaaatcca cgctacgccg caacgacgaa atcctgcccc tgttcgcacg cctgtcgcac
    23881 gccgagcagc acaaaatctt ccacccctca ggcgcgaaac gccgcatcgt gctggcaacc
    23941 aacgtcgccg aaacctcgct taccgtgccg ggcatcaaat acgtcatcga caccggcctc
    24001 gcacgcgtca aacgctattc cgcacgggcg aaagtggagc agcttcatat cgaaaaaatc
    24061 tcccaagccg ccgcccgcca acgctccggc cgctgcggac gcgtctccgc aggcgtatgt
    24121 atccgattgt tttcagaaga agatttcaac aaccgccccg aatttaccga ccccgaaatc
    24181 gcccgcagca acctcgccgc cgtcatcctg cgcatggcat cgctgaaact cggcgacgta
    24241 gcggcattcc catttttaga aatgcccgat tcgcggtata ttaatgatgg gtttcaggtg
    24301 ttgctggaat taggggcagt ggaggccgcc tgaaaacaag taggatatac acttctacct
    24361 gacaaagacc aacaaacgat aaaactacgg cacatctaga tgcccaacct acaacattgc
    24421 acacggtaaa aacatgaaac acttactcat cgattttgaa aacgtccagc cgcaaaactt
    24481 agacaaatta ctaaccgaaa atacccatat ttggctattt ataggtgtat tacacaaaat
    24541 gttacctatt agtctggtgc aatccctact acgtttcggc gaacgtgtcc atcttgtcca
    24601 gttacaaaaa acggggaaaa acgcattgga tttttacctg tcctattacc tcggacaaat
    24661 taccgccaca gaccccaatg cccaaatcgg catactctcg cgtgatggag gatacgatgt
    24721 tctggtcgaa catattttga aaaaccacca ggcgaagggt atcgtgcgcc tagccaatat
    24781 agatgaagta caacatcaga aaattactac cgaaccgccg tcagcattgc tggaaaacac
    24841 tcctcagcct gaaaccaccc tcaaaccaca gcaaccatta acttcctatt tccaagcagc
    24901 cctaactgca ctgcgccgcc ccgacgcttt ccgcccctgc cgcctgcata acctgcgaca
    24961 aaatctgcgt aagcatattt tgagtgattt gtttaaagaa aaaaccgatg aagaatgcga
    25021 aataaccact gctaacgtta tcaataaact caaagcacaa aacttcatca gcattgatga
    25081 acaggaaacc gtttcctacc atctcagtga taatgatttg ttacaaagaa tccaacgcca
    25141 tattttaagc caatgtccca aaacctacgc tgattttcaa gccgtcgtgc aaaaccgagc
    25201 agatgcactt cacttaacag tcggtaccaa cgacattcaa tcctttgcgc gacatttgcg
    25261 cgaccaaaac ctgatccgcc aaaacaatgg gaaaattgaa tatgcaccgt ttactgaacc
    25321 taaaccacag ccaacaccca agcagcctaa aaaaaccgca tgggaacctg atgaaattat
    25381 ttggaaaaaa gtgattgccg cgttatcgtt aaagaaccgt cctaataaaa ccaaaacttt
    25441 acgcaataca atccaggcac tcacaaaatc caatgcacaa gaaactgaca aactgctaca
    25501 acatttacaa ggtacccaag tcctacgtat tgacggtact aaaattgtgt atatcaagta
    25561 ggatgggcat ttatgcctga cctacaaaac caaaaacaac ctaacaccct taatcaactc
    25621 cacaaaccct tttcagacaa cctcgttttt tgaaaaacaa tctgtaaaca gataactgct
    25681 gaagaatacc gttgccgagc cccaaaacct gcctgcaact tttattgtga acttcccatt
    25741 atgaaaaaat cccttttcgt tctctttctc tattcatctc tacttaccgc cagcgaaatc
    25801 gcctatcgct ttgtattcgg aattgaaacc ttaccggctg caaaaatggc agaaacgttt
    25861 gcgctgacat ttatgattgc tgcgctgtat ctgtttgcgc gttataaggc ttcgcggctg
    25921 ctgattgcgg tgtttttcgc gttcagcatt attgccaaca atgtacatta tgcggtttat
    25981 caaagttgga tgacgggcat caattattgg ctgatgctga aagagattac cgaagtcggc
    26041 agtgcgggcg cgtcgatgtt ggataagttg tggctgcctg cgttgtgggg cgtgttggaa
    26101 gtcatgttgt tttgcagcct tgccaagttc cgccgtaaga cgcatttttc tgccgatata
    26161 ctgtttgcct tcctaatgct gatgattttc gtgcgttcgt tcgacacgaa acaagagcac
    26221 ggtatttcgc ccaaaccgac atacagccgc atcaaagcca attatttcag cttcggttat
    26281 tttgtcggac gcgtattgcc gtatcagtta tttgatttaa gcaaaatccc tgtgttcaaa
    26341 cagcctgctc caagcaaaat cgggcagggc agtgttcaaa atatcgtcct gattatgggc
    26401 gaaagcgaaa gcgcggcgca tttgaaattg tttggctacg ggcgcgaaac ttcgccgttt
    26461 ttaacccagc tgtcgcaagc cgattttaag ccgattgtga aacaaagtta ttccgcaggc
    26521 tttatgacgg cagtatccct gcccagtttc tttaacgtca taccgcatgc caacggcttg
    26581 gaacaaatca gcggcggcga taccaatatg ttccgcctcg ccaaagagca gggctatgag
    26641 acgtattttt acagtgctca ggctgaaaac caaatggcaa ttttgaactt aatcggtaag
    26701 aaatggatag accatctgat tcaaccgacg cagcttggct acggcaacgg cgacaatatg
    26761 cccgatgaga agctgctgcc gctgttcgac aaaatcaatt tgcagcaggg caggcatttt
    26821 atcgtgttgc accaacgcgg ttcgcacgcc ccatacggcg cattgttgca gcctcaagat
    26881 aaagtattcg gcgaagccga tattgtggat aagtacgaca acaccatcca caaaaccgac
    26941 caaatgattc aaacagtatt cgagcagctg caaaagcagc ctgacggcaa ctggctgttt
    27001 gcctatacct ccgatcatgg ccagtatgtt cgccaagata tctacaatca aggcacggtg
    27061 cagcccgaca gctatattgt gccgctggtg ttgtacagcc cgaataaggc cgtgcaacag
    27121 gctgccaacc aggcttttgc accttgcgag attgccttcc atcagcagct ttcaacgttc
    27181 ctgattcaca cgttgggcta cgatatgccg gtttcaggtt gtcgcgaagg ctcggtaacg
    27241 ggcaacctga ttacgggtga tgcaggcagc ttgagcattc gcgatggcaa ggcggaatat
    27301 gtttatccgc aatgagtggc gtaaaaacca ataaagacaa atttagatga tgtcggggaa
    27361 gatgcccgac cgacaagact atgcaaaata tgaaaaacca agtacgcgga tcaggcatgg
    27421 atgcccgatc caacccggcc aatgtttcag acgacctgca aaacagttcg ggccatatcg
    27481 gtgtcaacac gcgttaccgc ctgaccaaac tcggcgaaca aatggcgcgc ctgcccatcg
    27541 acccgaaaat cgcgcgcatt ttgctggcgg cgaagaaaca cgactgcatg gcggaaatat
    27601 tggtgatagc gtccgcgctg tcgattcaag acccgcgcga gcggccgttg gaagcgcgcg
    27661 atgccgcggc caaggcgcac gagcgtttta ccgacaagca atccgatttc cttgcctacc
    27721 tgaatatttg ggacagtttc cagcgcgagc gcgacaaagg tttgtccaac aagcagctgg
    27781 tgcagtggtg ccgccaatat tttctgtcgc acctgcgtat gtgcgaatgg cgcgagctgc
    27841 accaccagct tgcccaaacc gcgattgaaa tgggtttgac caccaaggaa accgctttca
    27901 gacgacctcc cgaaatcagg cagctcacgt cgtctgaaaa tgcgggtgac caagacttat
    27961 ccgccaagct caaacaaaaa caactggata aaaagcaaca ccgcgcccaa atccgcgccg
    28021 ccaaagaagc gggctacgaa caaatccacc gcgccctgct gaccggcctt atcgccaacg
    28081 tcggcatgaa atcgcccgac ggcaacgact acaccggcgc gcgcggcagc cgcttccacc
    28141 ttttccccgc ctccgccctg ttcaaagcca aacccaaatg ggtgatggcg gcagaactcg
    28201 ttgaaacgac aaaactttat gcgcgcgacg tcgccgttat ccagcccgaa tggattgagc
    28261 aggaagcccc gcacctcgtc cgctaccact atttcgagcc gcactgggaa caaaaacgcg
    28321 gcgaagtcgt cgccagcgaa cgcgtgacgc tttacggcct gaccgtattg ccgcgccgcc
    28381 ccgtgcctta cggcaaagtt gcccccgaag aagcgcgcga aatctttatc cgcagcgcgt
    28441 tggtggcgca ggaatgcgat ttgaaagcgg atttttttgt ccacaacaaa aagctgatta
    28501 aagaaattac cgaactcgaa cacaaatcgc gcaagcaaga cgtattggtc gatgacgaaa
    28561 cactgtttgc gttttatcac gaacggctgc ccaattttta tacggcggat gcggtttcag
    28621 acggtctgcg tcctgcaaat ccccagcaaa ctgccccctc ctacgcgagg gaagaacgga
    28681 gagagggcaa aacagttgcc gcacaaacca acttctccgc aaccgcagca aaccctctcc
    28741 ctaaccctct cccacaggag agggagcaga gtgccgcagc ttcagcggtt tcaaacgacc
    28801 tgcatcctgc aaatccccaa caaaccgccc cctcccccgt gggggagggt cgg
//
LOCUS       DAXHKX010000006        27419 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHKX010000006 DAXHKX010000000
VERSION     DAXHKX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27419
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.006"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..423)
                     /locus_tag="ACFMWZ_000258"
     CDS             complement(<1..423)
                     /locus_tag="ACFMWZ_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934282.1"
                     /translation="MNELNGSILSVKGLFFIYDETDLDIDVQRPDEIQFDLEGIPVLF
                     KCNGSEPNLSYEINKEAKNVSLGSYGELKIFDLTDFDFFGFLKDEDLLEMRKLYLDDD
                     EIGFRLIFSQSVLNMINWGDEFFLFKNLPEFLINENLHE"
     gene            complement(420..1253)
                     /locus_tag="ACFMWZ_000259"
     CDS             complement(420..1253)
                     /locus_tag="ACFMWZ_000259"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC5934283.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGSFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLQNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKNGLTNPSVKEGIRVN
                     K"
     gene            complement(1255..1998)
                     /gene="bioH"
                     /locus_tag="ACFMWZ_000260"
     CDS             complement(1255..1998)
                     /gene="bioH"
                     /locus_tag="ACFMWZ_000260"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC5934284.1"
                     /translation="MPDAAKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADIFGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRRLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            1997..2722
                     /locus_tag="ACFMWZ_000261"
     CDS             1997..2722
                     /locus_tag="ACFMWZ_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC5934285.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCAADLGAYR
                     TDAANSCPLCFRHIQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIHALKHLADLSM
                     VQPLADLMMQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKLGANRICCWTLARTPMKK"
     gene            2787..3251
                     /gene="trmL"
                     /locus_tag="ACFMWZ_000262"
     CDS             2787..3251
                     /gene="trmL"
                     /locus_tag="ACFMWZ_000262"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC5934286.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            3262..3384
                     /locus_tag="ACFMWZ_000263"
     CDS             3262..3384
                     /locus_tag="ACFMWZ_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC5934287.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3381..4100
                     /locus_tag="ACFMWZ_000264"
     CDS             3381..4100
                     /locus_tag="ACFMWZ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC5934288.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQAHGMVRAVLTAG"
     gene            4162..4488
                     /locus_tag="ACFMWZ_000265"
     CDS             4162..4488
                     /locus_tag="ACFMWZ_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC5934289.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4570..5154
                     /gene="ruvA"
                     /locus_tag="ACFMWZ_000266"
     CDS             4570..5154
                     /gene="ruvA"
                     /locus_tag="ACFMWZ_000266"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC5934290.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            5154..5291
                     /locus_tag="ACFMWZ_000267"
     CDS             5154..5291
                     /locus_tag="ACFMWZ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934291.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            5317..7173
                     /locus_tag="ACFMWZ_000268"
     CDS             5317..7173
                     /locus_tag="ACFMWZ_000268"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC5934292.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPRALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMNGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            7178..8101
                     /gene="rsgA"
                     /locus_tag="ACFMWZ_000269"
     CDS             7178..8101
                     /gene="rsgA"
                     /locus_tag="ACFMWZ_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC5934293.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENADLLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELPG"
     gene            8128..8436
                     /locus_tag="ACFMWZ_000270"
     CDS             8128..8436
                     /locus_tag="ACFMWZ_000270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC5934294.1"
                     /translation="MPSEILLFQTASGFQKCYNSVFTETPDTMKKITPKSFEEALSRL
                     ESLTQSMQGEMPLEDALAAYQEGNELVRYCQTKLAQVEQKLQVLDADGPKELNLESDE
                     "
     gene            8420..9316
                     /locus_tag="ACFMWZ_000271"
     CDS             8420..9316
                     /locus_tag="ACFMWZ_000271"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC5934295.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEANALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9425..9724)
                     /locus_tag="ACFMWZ_000272"
     CDS             complement(9425..9724)
                     /locus_tag="ACFMWZ_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC5934296.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9836..11281)
                     /gene="nuoN"
                     /locus_tag="ACFMWZ_000273"
     CDS             complement(9836..11281)
                     /gene="nuoN"
                     /locus_tag="ACFMWZ_000273"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC5934297.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRAHPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11291..12787)
                     /locus_tag="ACFMWZ_000274"
     CDS             complement(11291..12787)
                     /locus_tag="ACFMWZ_000274"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC5934298.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAILAVVVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(12876..14900)
                     /gene="nuoL"
                     /locus_tag="ACFMWZ_000275"
     CDS             complement(12876..14900)
                     /gene="nuoL"
                     /locus_tag="ACFMWZ_000275"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC5934299.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLILFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(14949..15290)
                     /locus_tag="ACFMWZ_000276"
     CDS             complement(14949..15290)
                     /locus_tag="ACFMWZ_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934300.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            complement(15414..15989)
                     /gene="fic"
                     /locus_tag="ACFMWZ_000277"
     CDS             complement(15414..15989)
                     /gene="fic"
                     /locus_tag="ACFMWZ_000277"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC5934301.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(16023..16328)
                     /gene="nuoK"
                     /locus_tag="ACFMWZ_000278"
     CDS             complement(16023..16328)
                     /gene="nuoK"
                     /locus_tag="ACFMWZ_000278"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC5934302.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(16325..16996)
                     /locus_tag="ACFMWZ_000279"
     CDS             complement(16325..16996)
                     /locus_tag="ACFMWZ_000279"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC5934303.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            complement(17032..17739)
                     /locus_tag="ACFMWZ_000280"
     CDS             complement(17032..17739)
                     /locus_tag="ACFMWZ_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934304.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            complement(17770..18249)
                     /gene="nuoI"
                     /locus_tag="ACFMWZ_000281"
     CDS             complement(17770..18249)
                     /gene="nuoI"
                     /locus_tag="ACFMWZ_000281"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC5934305.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(18330..19406)
                     /gene="nuoH"
                     /locus_tag="ACFMWZ_000282"
     CDS             complement(18330..19406)
                     /gene="nuoH"
                     /locus_tag="ACFMWZ_000282"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC5934306.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPRLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            complement(19409..21670)
                     /gene="nuoG"
                     /locus_tag="ACFMWZ_000283"
     CDS             complement(19409..21670)
                     /gene="nuoG"
                     /locus_tag="ACFMWZ_000283"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC5934307.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMANRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAVHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLQGFEYHDTAAI
                     LKDALDAESLPSKLDNRVSSTQKDFQTASSRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(21958..22395)
                     /locus_tag="ACFMWZ_000284"
     CDS             complement(21958..22395)
                     /locus_tag="ACFMWZ_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934308.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            complement(22395..23696)
                     /gene="nuoF"
                     /locus_tag="ACFMWZ_000285"
     CDS             complement(22395..23696)
                     /gene="nuoF"
                     /locus_tag="ACFMWZ_000285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC5934309.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(24086..24559)
                     /gene="nuoE"
                     /locus_tag="ACFMWZ_000286"
     CDS             complement(24086..24559)
                     /gene="nuoE"
                     /locus_tag="ACFMWZ_000286"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC5934310.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(24559..25815)
                     /gene="nuoD"
                     /locus_tag="ACFMWZ_000287"
     CDS             complement(24559..25815)
                     /gene="nuoD"
                     /locus_tag="ACFMWZ_000287"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC5934311.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLNDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(25805..26398)
                     /locus_tag="ACFMWZ_000288"
     CDS             complement(25805..26398)
                     /locus_tag="ACFMWZ_000288"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC5934312.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDN
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(26411..26893)
                     /locus_tag="ACFMWZ_000289"
     CDS             complement(26411..26893)
                     /locus_tag="ACFMWZ_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC5934313.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(26884..27240)
                     /locus_tag="ACFMWZ_000290"
     CDS             complement(26884..27240)
                     /locus_tag="ACFMWZ_000290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC5934314.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         6927 a         7603 c         6811 g         6078 t
ORIGIN      
        1 ttcatggaga ttttcattta ttaaaaattc gggtaagttt ttaaacaaaa aaaattcatc
       61 tccccaatta atcatattca acactgattg ggaaaaaatt agccgaaacc cgatttcatc
      121 atcatcaaga tatagttttc tcatttccaa taggtcttca tctttaagga aaccaaaaaa
      181 atcaaaatcg gttaaatcga atattttaag ctctccataa ctacctaaag atacgttttt
      241 agcttccttg ttgatttcat aagacagatt cggttcgctg ccattacact taaataaaac
      301 agggatccct tccaaatcaa actgtatttc atcgggacgt tggacatcaa tatcaagatc
      361 cgtttcgtca tatatgaaga aaagcccctt tactgataaa atacttccat ttaactcatt
      421 catttattta cccttatccc ttcttttact gatggattag ttaagccatt cttcggaaaa
      481 aactgcacca cgttctcccc ttgcggcagc atcagttttt tcacggcgtg tccgtacacc
      541 gtttccagcg taatgcccaa accgccttcc cgctcaaata ttgtcccgac acacgaacgc
      601 gcggcgtttt catcgttcca cgccatcgcc cgccaaacgc ccagcgtgtc catatccgcc
      661 caaaacgttt ccgccttttt gtaatccaac accagcttcg ccgtatcggt aacggggtcg
      721 taaaagccgt tttcagcaag catatcgccc aagtcgtgca tatcgggaaa catcacgctg
      781 cggctttcaa tgccgttttc tttcagacgg catttcagtt cggtaaaagt gtcgcgcccg
      841 aagcaggtaa aaaacagcag cccgtccgtc ttcagcgcgc gcgcccaatt ttgcagcacg
      901 ggaaggattt gttccgccgc caacagtccg agattcgacc acaacatatc ggcacacgct
      961 tcgggcaacg gcgcggtcgg ggattggcag tgttgcacca cgcccttacc cgtaaacctt
     1021 tgccaaaaac tgccttttcg ggcggcggcg gcggccgcca aaaaatccgc acgggaatcg
     1081 tattcttcaa ataccgcctg cggatagcgt ttcgccagca ggctgcggct gatgtccgca
     1141 tccgcacctg caagcaggat atacttgggc gcgttgcgga cgagtgtcag gcgttggtcg
     1201 gcatattcgg cgagatggcg gtgaacctgc cagcatttgt cctgcggatt catctcaaac
     1261 cccttcaaca aagtcgcggt acagcgcggc aaacgcttcc gcgtggctca aaaacggcgc
     1321 gtgtgccgcc ttttccatca caaccagcct gctgcccttc aaacggcggt gcagatactc
     1381 gcccatgcgc ggcggcgtaa tcgcgtcttt gccgccgaac accagcagta ccggaacatc
     1441 tatcttgtcc aacaaatgcc gcgcatccgc cctttccgcc gcgtccaaag cttcctgtaa
     1501 ggcggagggc gtgccgcagc gcgccaaatc gggcaggatt ctgcctatga ttccgtccgc
     1561 atcaggcgtg tgcaaaagct ggagttgtag aaactgtttg atatgtttgg cataatccgt
     1621 tcggaacgcg ccgaccattt tgcccagtgc aggcgcggca agcccttcgg gataatcttc
     1681 ggcagccgtc agccgtgcga aactcgccgt caggcagagc gaacggactt tgtcgggatg
     1741 gcgcgccgcc agatacagcg cgaccaatcc gccgagcgac cagccgaaaa tgtcggcggg
     1801 cgtatcgatt tgtgcggcaa cagcgtcggc ggctgcctca atatcgaagg gttgcgcgaa
     1861 gggcgcgtcg ccgtgtccgg gcaaatcgac ggcggacacc ggccacgttg caggcaggcg
     1921 cggcatcaaa tcgtcgaaca cgtggcggtt cgccccccac ccgtgtatca ggtaaacttt
     1981 tttggcagca tcaggcatga attttctctc ttgttggcgg cgcatcgcag acgcgcccac
     2041 tatcaggcgt tgcgtattat gccacggttc gtccggcgtt tcagacggca tctgcgccgg
     2101 ctgcgcggcg gatttggggg cataccgcac cgacgcggca aacagctgcc ccctgtgttt
     2161 cagacacatc caaggcggcg cggtgtgcgg cggctgtcag aaaaaaccgc ccgcattcga
     2221 ccggatgtgg gcttcgctgc attacgaacc gcccgtcagc aatatgatac acgcgctgaa
     2281 gcacttggct gatttgagca tggtgcagcc gctggcagac ctgatgatgc agaatccgcc
     2341 cgaccggctt gcagacgaat gtttcgattt cgtcctgccc gtaccgctaa gcagggagcg
     2401 gctgctgcaa cgcgggttca accaaagtga aagcatcgtc gggctgctgg cacaacgcta
     2461 cggctggcag atactgcccc gacacaccgt tttccgacac caccgcccgc cgcaaagcac
     2521 gctcaaaggc ggcgaacggc ggcgaaacat caaaaacgcc tttgaaatcc gcacaccgat
     2581 accggaaaac tgtaatattc tgttaatcga cgatgtcttt accaccggcg cgacgctgga
     2641 cgaattggca aagacgctga aaaaattagg cgcaaaccgc atctgctgct ggacgctggc
     2701 acgcacgcca atgaaaaaat aactaaattt tttgacaccc catccgcctt gtggcaaggt
     2761 tacgcgccta taagtgattg atatttatgt ttaccatcgt tttataccaa ccggaaatcc
     2821 cgccgaacac gggcaacatc atccgcctgt gcgccaatac cggcgcggat ttgcaccttg
     2881 tcaaaccgct cggcttccca ttggattccg ccaaaatgaa acgcgccggg ctcgactacc
     2941 acgaattcgc cagcctgacg gtgtacgaaa acttcgacga ctgcctcaag gcactcgcag
     3001 gcaggcgcat tttcgccctg accaccaaag gcacggcgcg ccccgatgaa accgcgtttc
     3061 aaaaaggcga cgttttactg ttcgggccgg aaacgcgcgg actgcctgcc gacatcctcg
     3121 acagcctgcc cgccgcgcaa aaaatccgcc tgcccatgca gcccggcagc cggagtatga
     3181 acctttccaa taccgttgcc gtgattctct ttgaagcgtg gcggcaacac ggttacgcag
     3241 gcggcgtttg aacgcaggtt catgccgtct gaaaacctat ccggacacat tccgaaccgc
     3301 cgtccgcact gtacggcggt tcggaacgta tccggcgggc atgatgccca ttcgtcttca
     3361 actcaagaac ggaacacgtt ttgactttaa cccgaaaaac ccttttcctt ctcaccgccg
     3421 cattcggcac acactccctt cagacggcat ccgccgacgc agtggtcaag gcagaaaaac
     3481 tgcacgcctc cgccaaccgc agctacaaag tcgccggaaa acgctacacg ccgaaaaacc
     3541 aagtcgccga attcacgcaa accggcaacg cctcgtggta cggcggcagg tttcacgggc
     3601 gcaaaacttc cggcggagaa cgatacgata tgaacgcctt taccgccgcc cacaaaaccc
     3661 tgcccatccc cagctatgtg cgcgtaacca ataccaaaaa cggcaaaagc gtcatcgtcc
     3721 gcgtcaacga ccgcggcccc ttccacggca accgcatcat cgacgtatcc aaagccgccg
     3781 cgcaaaaatt gggctttgtc agccaaggga cggcgcacgt caaaatcgaa caaatcgtcc
     3841 cgggcaaatc cgcaccggtt gccgaaaaca aagacatctt tatcgacttg aaatctttcg
     3901 gtacggaaca cgaagcacaa gcctatctga accaagccgc ccaaaacttc gccgcttcga
     3961 catcaagccc gaacctcgcc attgaaaaac gccgttacga atacgtcgtc aaaatgggtc
     4021 cgtttgcctc gcaggaacgc gccgccgagg ccgaagctca ggcgcacggt atggttcggg
     4081 cggtattgac tgccggctga cggttatttg ataccttatt aatatccaca ttttccaacc
     4141 cacgatttac aaaggcaaaa tatgaacatc aaacaccttc tcttgaccgc cgccgcaacc
     4201 gcactgctgg gcatttccgc ccccgcactc gcccaccacg acggacacgg cgatgacgac
     4261 cacggacacg ccgcacacca acacagcaaa caagacaaaa tcatcagccg cgcccaagcc
     4321 gaaaaagcag cgttggcgcg tgtcggcggc aaaatcaccg acatcgatct cgaacacgac
     4381 aacggccgtc cgcactatga tgtcgaaatc gtcaaaaacg gacaggaata caaagtcgtt
     4441 gtcgatgccc gtaccggccg cgtgatttcc tcccgccgcg acgactgaat ttgatacaat
     4501 ccgtgccgtc tgaagcccga accggtttca gacggcattt tgcacccgac acttcaggat
     4561 tcggcataca tgatcagcag actgaccggc aaactggttg aaaaaaaccc tccgcaaatc
     4621 gtcatcgatg tcaacggtgt cggctatgag gccgacgtat cgatgcagac cttctacaac
     4681 ctgccgcccg tgggtgaaag cgtacaactg tttacccagc ttatcattcg ggaagacgca
     4741 catcttttat ttggttttgc cactgcggaa gaacgcaaaa cattccgcca actgattaag
     4801 gtcagcggca tcggcgcgaa aacggctttg ggcattttgt cggcgatgac ggcagacgag
     4861 ctggcgcagg cggttgcaga agaagatgtc aaacgcctct cctccgcccc gggaatcggc
     4921 aaaaaaaccg ccgaacgcat ggttttggaa ctgcgcggca agctggtcgc gcatacggta
     4981 acggacgggc tgtttgccgc cgcacccgcc gccgacgaaa cggaagacat cgtcagcacg
     5041 ctgcttgcgc tgggttacag cgaacgcgaa gcaaaagcgg cggtcaaagg cgttccggag
     5101 gggacggacg tgggcgaagg cgtgcgcctt gctttgaaaa acctgctgaa ataatgccgc
     5161 ctgaaggcgg cgcggcgttt gccctgacga aaccctgcgt ttccgcctcg cgctgtcttc
     5221 ccgacggctt ttcccgtaaa atggcgttat tgtccttcct ttcagacggc attgtgtttg
     5281 atgccgtctg aaactgtttt accgaaatcg aaaacaatgt tgaacaaaat attttcctgg
     5341 ttcgagtccc gaatcgaccc ttatcccgaa gccgccccga aaacgccaga aaaaggcttg
     5401 tggcggtttg tctggagcag catggccggc gtgcggaaat ggatagccgc cctggctgcg
     5461 ctgaccgccg gcatcggcat tatggaagcc ctgatttttc aatttatggg caaaatcgtg
     5521 gagtggctcg gcaaatacgc gcccgccgaa ttgtttgccg aaaaaggttg ggaactggcg
     5581 gcaatggcgg cgatgatggt gttttcggtc gtgtgggcgt ttgccgcgtc caacgtgcgc
     5641 ctgcaaaccc ttcagggcgt gttccccatg cgcctgcgct ggaacttcca ccgcctgatg
     5701 ctgaaccaaa gcctcggttt ttatcaggac gaatttgccg gacgcgtgtc cgccaaagtc
     5761 atgcagaccg cgctggcgtt gcgcgacgcg gtgatgacgg ttgccgatat ggtcgtttat
     5821 gtgtcggtgt atttcattac ctccggcgtg attctcgcct cgctcgactc atggctgctg
     5881 ctgcccttta tcggctggat tgtcggtttc gcttcggtga tgcgcctgct gattcccaaa
     5941 ttggggcaaa ccgccgcatg gcaggcggat gcccgctcgc tgatgaccgg ccgcattacc
     6001 gatgcctatt ccaatatcgc caccgtcaaa ctcttctccc acggcgcgcg tgaagccgcc
     6061 tatgccaagc agtcgatgga agaatttatg gttacggtgc gcgcccaaat gcggctggcg
     6121 acgctgctgc attcgtgcag cttcatcgtc aacacctccc tgaccctctc caccgccgca
     6181 ctgggcatct ggctctggca caacgggcag gtcggcgtgg gcgcggttgc tacagccacc
     6241 gccatggcgt tgcgcgtcaa cggtttgtcg caatacatta tgtgggaatc cgcgcggctg
     6301 tttgaaaaca tcggcaccgt cggcgacggc atggcaaccc tgtccaaacc gcacaccatc
     6361 ctcgacaagc cccgggcact gccgctgaac gtgccgcaag gcgcaatcaa atttgaacac
     6421 atcgatttct gctacgaagc cggcaaaccg ctgctcaacg gcttcaacct caccatccgc
     6481 ccgggcgaaa aagtcggctt gatcggacgc agcggcgcgg gcaaatccac catcgtcaac
     6541 ctgcttttgc gcttctacga accgcaaagc ggcacggttt cgatcgacgg gcaggacata
     6601 agcggcgtta cccaagaatc tttacgcgcc caaatcggtt tggtcacgca agatacctcg
     6661 ctgctgcacc gttccgtgcg cgacaacatt atttacggcc gccccgacgc gaccgatgcc
     6721 gaaatggttt ccgccgccga acgcgccgaa gccgccggct tcatccccga actttccgat
     6781 gccaaagggc ggcgcggcta tgacgcgcac gtcggcgaac gcggcgtgaa actctccggc
     6841 ggtcaacgcc agcgtatcgc catcgcccgc gtgatgctca aagacgcacc gattcttctt
     6901 ttggacgaag ccaccagcgc gctcgattcc gaagtcgaag ccgccatcca agaaagcctc
     6961 gacaaaatga tgaacggcaa aaccgtcatc gccatcgccc accgcctttc caccatcgcc
     7021 gcaatggaca ggctcgtcgt cctcgacaaa ggccgcatca tcgaagaagg cacacacgcc
     7081 gaactcctcg aaaaacgcgg gctttacgcc aaactctggg cgcaccagag cggcggcttc
     7141 ctcaacgaac acgtcgagtg gcagcacgac tgaaccgatg ccgtccgaac acccgttttc
     7201 agacggcatt tccacaccca accccaaaga aaccatgaac gacaccgccc aaattaccgc
     7261 cagctacggc cgccgctaca tcgtgcgcac gcccgacggc acaacctacg aagccagcac
     7321 gcgcaaaaaa cgcgtcgatt tcgcctgcgg cgaccgtgtc cgcatcagcc ccgtcaacgc
     7381 cgaacaagtt gtgattgaag attttttacc gcgccaaagc ctgctctacc gccaagacgc
     7441 gtggaaaacc aaactcatcg ccgccaacgt tacccaactc cttatcgtta ccgccgccgt
     7501 cccgagtccg agcatgcggc tgctgcaacg cgccctgctt gccgccgaag ccgccggtat
     7561 tgaagccgtc atcgtcctga acaaagccga cctgcccgaa accgcccttt ggcgcgaaaa
     7621 actcaaattc tacgaaacgc tgggttatcc cgtcatcgaa acccgtgcgc tggaaaacgc
     7681 cgacctcctg cgccccgtcc tgcaagggca cagcaacatc ctgctcgggc agagcggtat
     7741 gggcaaatcc accctgacca acgccctttt aggcagccaa accgcccgca ccggcgacat
     7801 ttccgccgca ctcgactcgg gaaaacacac caccacccac gcccggcttt atgatttgaa
     7861 cggcgaaacc caactcatcg actccccggg tttgcaagaa tttggtttac accacctcca
     7921 agccgccgat ttgccgcgct atttccccga tttccgccac cttgtcgggc aatgccgctt
     7981 ccacaactgc acccaccgcg ccgaacccgg ctgcgccttc aaagccgccg ccgaaaccgg
     8041 ggcggcaagc cccgaacgcc tcgccttttt gcagggcatc accgacgaac tgcccgggta
     8101 acgccttgcc gtttgcagga caaaacaatg ccgtctgaaa tcctgctgtt tcagacggca
     8161 tccggttttc agaaatgcta caattccgtt tttacagaaa ctcccgacac catgaaaaaa
     8221 attaccccga aatcgtttga agaagccttg tcgcgccttg aatcgctgac gcagtctatg
     8281 cagggtgaaa tgcccttgga agacgcgctt gccgcctatc aggaaggcaa cgagctggtc
     8341 aggtactgcc aaaccaagct ggcacaagtc gaacagaaat tacaggtttt ggatgcggac
     8401 gggccgaagg agttgaacct tgaatccgac gaatgatttg aaagcgtggc aacagagggc
     8461 gcaggcacag acagagctgc tgctggaacg ctttttgccg tctgaaaacg aaatcccgca
     8521 cacgctgcac gaagcgatgc gctatgcggc tttggacggc ggcaagcgtc tgcgtccgat
     8581 gctggttctg gcagcttcgg aattaggcga agccgtgcat gaagcagtag aacaggcaat
     8641 ggcggcaatc gaaatgatcc acgtctattc tttggttcac gacgatatgc cggcgatgga
     8701 caacgacagc ctgcgccggg gcaaaccgac ctgccacatc aaatatggcg aagcaaacgc
     8761 actgctgacc ggcgatgctt tgcaaaccca agccttcgac gtgttaagtc gtccgacaga
     8821 actgcccgcc gcacgccagt tggcaatgtt gtcggtgttg gcgaaagcgg gcggcagcag
     8881 gggtatggcg ggcggtcagg caatcgattt ggcgaatgtc ggcaaacaaa tggttcaagc
     8941 cgatttggaa cagatgcaca gcctgaaaac gggtgcgcta atccgtgcgg cggttttatt
     9001 gggggcgacg gcgtgtcctg atctgtcaga tgcggaactt tccgtattgg acgcttacgc
     9061 ggcaaaactg gggctggcgt tccaagtcat tgacgatgtg ttggattgtg aagcggacac
     9121 ggcgactttg ggcaaaacgg cgggcaaaga cgcagacaac gacaagccga cttatgtgaa
     9181 actgatgggc ttggaagcgg cgcgctcata cgcacacaaa ctggttgccg aagcggtcgc
     9241 gctgctcgaa cccttcggcg acaaagccct gcgcctgcgg cagttggcag aatttgcagt
     9301 cgcccgcaaa tattaaaacc ggcgtatatc gccggctgaa acgtgccggc acagcgatgc
     9361 cggcctgcac ttgaatgaaa caaatgccgt ctgaacaggt ttcagacggc attcctatgc
     9421 ctgcctattc cctgttgcgc gtgtgccaaa aataccgcca cacgaaacag ggaaacgcaa
     9481 aaatcaggta caggcagacg acggtggcgt aaaactccca accctgatcg tgtaccgctc
     9541 ccgcacggga ttcgaggatg taggcaagaa cggcggtcag cgcgaaacct gccgccagct
     9601 cgatcaagtg gtgcccgaaa tgtttgcgct tgagcggtac gatgccgaac agcctggtcg
     9661 tgaggaaggg ggcgttggca aaaatcaagg caagcagcaa aaggatgtac atggatgcgg
     9721 tcatggcggg ctttctgttt gggcattgtc ggcggcgatg ccgtctgaac tgcattgcgg
     9781 aacggcatat cttaacaaaa acggcagcct ctgacacggc tgccgttgcg gcggcttaca
     9841 gcgtgttttc caacgccttg gcgcaccagt cgataacggt ttgcggcata atgccccaca
     9901 gcaccaacag cagcgcgttg acgctcaaaa ggaattttgc ggcattgttg ccgccgacgg
     9961 tgggatgggc gcggccggat tcgtcaaaat acatgacttt gaccacgcgc aggtagtaga
    10021 acgcaccaat cagcgacatc acgacggcaa atacggacaa ccaaacatag ccttgtttca
    10081 agagtgccat aatcacgccg aatttggcgt aaaaacccat cagcggcgga atgcctgcca
    10141 tggagaacat aaccagcagc atcaaaaagg caagccatac gcggtgttgg ttcagccctg
    10201 ccaaatcgct gatgttttcg cactcgttgt ccccatccga caacaccatc aacacgccga
    10261 accctgccgc cgccatcagc gcgtaggtaa tggcgtaata gagacccgcc gcaaagccga
    10321 ccgcgcctgc cataaacgcc aacaagataa agcccatatg cgatacggtg gaataggcga
    10381 gcatacgttt gatattggtc tgcatgatgg cggcaaggtt gccgaccagc agcgaggcgg
    10441 cggcaagcag ggcaaacatc agagaccagt catgatgcac ggttcccaac ccggtaacga
    10501 ggatgcggaa agcgaaaacg acggcggcga ctttcggggc agtgccgact agggcggtaa
    10561 cagaagtggg cgcgccgtga tacacgtcgg gcacccacat atggaacggc accgcgccga
    10621 gtttgaacgc aacggcgacg acgataaaca ccagacccag tttcaacagc cattcgtttg
    10681 cttcttcatt gaaggaagag gcgagcacgc cggcaaattc cagcgaaccg gttgcgccgt
    10741 aaaccataga aataccgtag agcagcaggc cggatgccag cgcgcccaga acaaaatatt
    10801 tcaaggcggc ttcggcggca aagccggaat cgcggcgcag ggcaatcagg gcgtaaaggg
    10861 cgagcgacaa gagttccaaa ccgatatagg cagttaaaaa atgccccgcg ctcaccatca
    10921 cgctcatgcc caagagggca aacagcgaca gggtgtaaaa ctcgcctttg aaaataccgc
    10981 gcacttggtt gtagggcttg gcataaacaa acagggcaaa cgtcaaggca tataaaacca
    11041 tttttgccaa acgcgacata ccgtctgcaa tatacatgcc gttgaacgaa gacgtgctgc
    11101 cctgttccca aacggcaagc tgcaccacag ccgtaaccgc caccgttgcc aacgcgccgt
    11161 aatgcgtcca ccggcgtttg tcatcactga cccacaagtc cgccagcaac aataacacca
    11221 gcagcgcaag cagcacgatt tcgggcatgg cgggcatcaa attcaaatca gaccagttca
    11281 tttacacacc tcaaatcttg ctttgtgcca catgggcaat caaatcgttt gccgcctgat
    11341 gcaccacttc gataaatgcg ttcggataca gacccatacc caataccaca accgccaaaa
    11401 tcgccagaat cgcaaattcg cggcagttga tgtctttcat ttcggcaacg tgcggattgt
    11461 ggatcgcgcc gaaaatcacg cgcttgtaca tccacagggt ataagacgca ccgtaaatca
    11521 gggtcatggc cgccaacgcg cccacccaga aattcacttt aaccgcgccc ataatcacca
    11581 taaactcgcc cacgaagccg gaagttgcag gcagaccggc attggccata ccgaacaaca
    11641 tcataaacgc cgcaaactta ggcatcacat tgaccacgcc gccatagtca gcaatattgc
    11701 gggtatgcag gcggtcgtac atcacgccga tacacataaa catcgccgcc gacacgaaac
    11761 cgtgcgaaat catttgaatg attgcgcctt tcaacgccca gtcgtccaac tgcccgtcaa
    11821 caaacaaaaa catcccaagc gttacaaaac ccatatggct gatagacgaa tacgccacca
    11881 gttttttcat atcggtttgc accaaagcca ccataccgat ataaatcacg gcaatcaggc
    11941 tcaatacgat gatcacaggg gcaaaatagc gtgccgcatc cggcataatc ggcaggataa
    12001 aacgcaagaa accatacgca cccagtttca gcgtaattgc cgccaacacc atcgaaccgc
    12061 cggtcggcgc ttcaacgtgg gcatccggca accaagtgtg cacagggaac atcggcactt
    12121 ttacggcaaa tgacaggaag aatgccacaa acaaaagctg ttgtacgccc aatggaatct
    12181 gcttgatgtt ttggaaatcg acaatagaga agctgcctgt ctgatagtac aggtaaacca
    12241 tggcaaccag catcaaaagc gaacccatca gcgtatagag gaacagcttg accgacgcat
    12301 aaacgcggcg cgggccaccc catacaccga taatcaggta cagcggaatc agcataccct
    12361 cgaagaacac ataaaacaga atcgcatcct gcgcggcaaa cgcaccgtta atcaaacccg
    12421 acatcatcag gaatgccgcc atatactgcg ccgggcgttt ctgaataact tcccaacccg
    12481 ccagcaccac caacagcgta ataaacgcat tcaagatgat aaagagcact gaaataccgt
    12541 ccacgcccaa tgcatagttg attttcagca gcggaatcca ctcgtggaac tcggtaaatt
    12601 gatagccgcc gctcaaacgg tcgaaaccgg taaacagggg cagcgttacc aagaaaccgg
    12661 caagcgcacc cataaaggca agcacacgcg ccagcggcgc acggctgtcc ttaccggtcg
    12721 acaaaaccag cacacctgcg gcgatgggta tccatattgc caagctgagt aggtagttgg
    12781 aaaacatagt ggttaacctg tggttaaaat aaaaatatcg ttgtacgggt tggtttgtca
    12841 gacgacgttt taagagaccg tctgaaacct catccttatc tgaacaatcc ccagaaggtc
    12901 ataccgagca agaccaatac gccgaacacc atagcggcgg cgtaggtata gataaagccg
    12961 gtttgagctt tacgcacttg cgcggcaatc gcgccgacca gtttagcaga gccgttgacg
    13021 atgccgttgt cgataatggc ggtatcgccg actttccaga agaaagtacc caatgcgcgt
    13081 gtgcctttgg cgaaaacgtt gaaatacagg gcgtcgaggt agtatttgtt ttcaaacaaa
    13141 acgtaaatcg gacggaacgt ctgtgcaatt ttcgctggca ggtgcggcag tttgacgtac
    13201 aaaagccatg cgctcaacac gcctgcgata gcaaggtaga gtacaggcga atgcaggctg
    13261 tgcgacacca ttgccaatgc gccgtggaac tcttccttca tgatgtgcat ggtcggatgc
    13321 gcgtcggcgt tgacgaaaat cacatctttg aagaaatcgc cgtaaagcat aggctcgatg
    13381 gcaacgtagc cgatgatgac ggacggaatg gcaagcaaaa tcaaaggcag ggtaacaacc
    13441 aacgggcttt cgtgcggatt gtcgtttttg cccaagctgt gatgttcttc gccatgatga
    13501 tcgtcatgat gttcaggcaa gctgcgccat ttctcttcgc cgtggaacac cataaagtat
    13561 tggcggaacg cgtagaacgc ggtaacgaac acgctggcaa ggacggcaaa ataggcaaag
    13621 ccgctgcccg gcagggtgct gtatttcgcc gcttcgataa tcgaatcttt agaatagaag
    13681 ccggagaaga acggcgtacc gatcagcgac aagttaccga tcagcatggt cagccaagta
    13741 atcggcatgt attttttcag gttgcccatg tggcgcatgt cttggtcgtg gtgcataccg
    13801 ataatcgcgc tgcctgccgc caagaacaac aaggctttaa agaaagcgtg ggtcatcaca
    13861 tggaacatcg ccacggaata ggcagacgca cccaaagcca cggtcatata gcccagttgc
    13921 gacaaggtgg aatacgcaac cacacgtttg atgtcgtttt gaatcacgcc caagaaaccc
    13981 ataaacaggg cggtaatcgc accaatcacc atgatgacag acagcgcagt gctactcatc
    14041 tcataaatcg gcgacatacg cgataccatg aacagaccgg cggtaaccat ggttgcggcg
    14101 tgaatcaatg cagaaatcgg ggtcgggcct tccatcgaat caggcagcca aacgtgcagt
    14161 gggaattgtg ccgatttacc catcgcgccc acaaacagga tcaggcaggt tacggtaatc
    14221 aaagaccatt ccacaccggg gaaaagctgg atggtggcat tttggacgtt gggcagataa
    14281 gcgaatacat cttgatagcg caggctgccg ccgaaatagg caagcaccaa gccgataccg
    14341 agcaaaaagc cgaagtcacc gacacggttg atcaaaaagg ctttcaggtt ggcaaatgtt
    14401 gcgctcgggc gtttgaaata gaaaccgatc aagagatacg ataccaagcc cacagcctcc
    14461 cagccgaaga agagctggat gaagttgttg ctcataatca gcatcaacat actgaaagta
    14521 aacaaagaaa tatagctgaa gaagcgttgg tagccgactt tttcatcgtg catatagccg
    14581 atggtgtaga tatgcaccat caacgacacg cccgttacca cgaccatcat catcgccgtc
    14641 atcgtatcga ccaagaagcc gacggaaaaa tccaagccgc ccattgtcag ccaggtatag
    14701 acgttttcgt caaacttggc gcggctgccg tcgataaagc cccacagcac ataagccgac
    14761 aacacggcag acaccgccac accgagtatc gtaaccgtat gcgcgccggc acgtccgatt
    14821 ttgttgccga ataaacccgc aatcagcgag cctgccaaag gcacgagggc gatgatgaga
    14881 tataaagtca tgtcgttcat ttgattgaat ccgattgatt taaaaatcta tgtttgtttc
    14941 gtacaaaatt acttcggaga aacaaatcca acacgctcca atcgtttgcg tgccacagct
    15001 aattgctctt cagtaaataa atcacaccac ggcttttgta acaccagata ttccatactg
    15061 tatttcaagg cgtggactcc gccaccactc aaaatcagct ctgtaaaacc ggtctgagtc
    15121 ttcttttccc ccgtacttaa taatttatcc gccgcctctt taccaccaaa ttcatttaca
    15181 atttgtaaaa atcgtgtcgc cttgtaaggt tgcggcaaat tcaaagcctc ctgataaata
    15241 tttaacatgg ctttatgaaa ttcttgttct aactgatttt tatccatcat tcttcttcca
    15301 atatttcaga ccggattatt cttacccaga atttcttttc tcatccgctc ccgtctgatc
    15361 acctaccaaa tcaggtcgtc tgaaacagtc tgaaatcgct tttcagacga ccttcagcct
    15421 ttttcatacc cttcgtaata atacgactgc tcgatacctt taaagatgat ttcacggttg
    15481 tccacatcgt cggtcaggtt gtcctttaac agaaagcgca gttctaaatc gttgacgggg
    15541 ctgcgttcca tcgcctgcaa atacagggtt ttgcttacat tttgccagtt cacgactttt
    15601 ttcaggtttt tcttcagcac caaatccagc cagatgcggg tacttctgcc attaccctcc
    15661 aaaaacggat gggcaatgtt catttcaaca tatttggcga tgatttcttc aaaagtccgc
    15721 tcgggcatct gctcgatttt aaccaaagcc tcttttaaat acatggcgtt ggcaaaacga
    15781 aaaccgcctt tggaaatgtt gtcttccctg atttgacccg caaaatcata taagccgccg
    15841 aacaggtaac ggtgaatctg ttgcaggccc gcggtggtac cgacttcgat acggtcgatg
    15901 tcgccgcttt caaacaggcg gcgggcatta tgcaggcttt gttcgtctat ggatttcatc
    15961 gtttttccta tcgggttttc agacggcatc ggtgtcttgt caggtctcga cccaacctac
    16021 ctttaccctt tcaactcgtc caaatcggca acgttaatcg tttgtcggtt gcggtacacc
    16081 agcaccataa tcgccaaacc gatggcagat tcggcagcag caacggtcag tacgaaaaat
    16141 acgaaaattt gtccggcagt atcgcccaaa tgttgcgaaa aggcgataaa gttgaagttc
    16201 actgccaaaa gcatcagctc gatggacatc agcaagacca gcacgttttt gcggttcata
    16261 aagataccca tcgcgctgat accgaacagg agcgcaccca ataccagata atgcgtcaag
    16321 gtaatcatgc tttgccctcc tctttcggct tgaggtcgtc tgaaacttcg ctttcttcgg
    16381 cagattcggt ttgcggtttc acggcttcca ttttcaccaa gcgcatacgg ccttggtcgg
    16441 cgcgtacttt aacttggtcg gcaggatcca tacgtttcgg attaaccgtt ttacggtgaa
    16501 ccagcgcaat cgcggccacc atacccaaca gcagcaatac cgccgccaat tcaaacggca
    16561 gcagataatc ggtataaata cggctgccca aatcgcggat attgttgtaa tcggcgggaa
    16621 tgtctttcat cagcccaaat gcggcaaggt cggttttcgg attgaccaga atcagaatca
    16681 gggcaactgc caacaatgtg ccgaccacac cggcaacagg cgcgtgccgc cagaaaccgg
    16741 cacgcatttc ttcaatgtcg atgttcaaca tcatcacgac gaacaggaac aataccatca
    16801 ctgcgccgac atacacgacc accagcgtaa cgcccaaaaa ctccgcctgc atcagcatcc
    16861 aaatcatcgc gctcacgcag aaggtcaaca ccagatgcaa agcggcgtga acaggatttt
    16921 tagcggtgac ggttttgatc gcgccataaa gaattatcac tgcaaaaata taaaataaaa
    16981 tcagttggaa agtcatagtc tatgctttct ttattaatca aaaatatggt ttcaagcagt
    17041 ctgaaaatcc tggttacgca aacaatcaaa cttaatcagt tgatccatat ccgcacaaat
    17101 ccacgttgtc gcaattttga ataacaacat ttctatgtga tacggttttg cttgtttacc
    17161 ataaacttgt ttcgccaaat cttgtaacaa tccacaatat tgatgaaacg aaacgtctgg
    17221 tttccgataa ttcggtttga tatcagaaaa atgaaacaat acgttcatat ttcggatttc
    17281 tagttcccta tttctacctt gtggctgaaa aaacaaatct gcatttgtaa atttaaacaa
    17341 caaccaattc aacgaaaaaa tagcgcgtga atcataaatg gcgaatcgct cacaatctat
    17401 aaaagaagcc aatttagata atgatgaaat tttcaacaaa ccactactca attttcccga
    17461 attaagttcc gataaaaatt gacgaataag ctggctattt tcagcagatt gtttaaaact
    17521 tttaatccca ccccattcac ggataatcca ataatgcgtc ttcaagctat catcattttg
    17581 taattttggg gataaatttt cttttaaaaa aatattttgt tcaaaattac ttaatccact
    17641 tggcaactca atcggactat ttctagttct aatttcttca aaattatcta ttggctcatg
    17701 ttcggctaaa tatttcaatg cattaatcag tccttccata tttaaaacct tccaagaagc
    17761 cccaaagcat taacgatacg gcgcgtcagc ggctttgcgt ttggctattt cagcttcgta
    17821 tttatcgcca atggccaaaa gaatcggctt ggtcatgtgc aagtcgcctt ttttctcgcc
    17881 gtggtattca aaaatatggg tttccacaat cgcatcggtc gggcaggcct cttcgcagaa
    17941 accgcagaag atgcacttgg tcagatcgat gtcgtaacgc ttggtgcggc gggtaccgtc
    18001 ttcacgttct tccgattcga tgttaatcgc cattgccgga caaactgcct cacacaattt
    18061 acacgcaata cagcgctctt cgccgttcgg ataacggcgt tgcgcgtgca ggccgcggaa
    18121 acgcacggat tgcggcgttt tctcttcggg aaaataaatc gtgtctttgc gggcgaaaaa
    18181 gtttttgagc gttacgccca taccttttac caattcgcca agcagaaagg tttttactaa
    18241 gttagccata ttatgttccc tcaaaacagg gatttcgtta ggtattcaaa atcgctttgt
    18301 tcagacggcc tcaagatgcc gtctgaaact tatttccaca aattcagcgg tgaaatcatc
    18361 cacacgccca aaaccacgat gtaggcgaag ccgatcggaa tcagcacttt ccagcccaaa
    18421 cgcatgattt ggtcgtaacg gtagcgcggg aaggttgcac ggatccacag gtaccagtac
    18481 aacaccgccg ccattttcac gaacatccag aatgcggaag gtgtgccgat aaagccccaa
    18541 ctttgcggga acggagacag ccagccgccg aggaacatca gcgatgtcag cgcggcaatc
    18601 agaatcatga aaatgtattc ggcaaggaag aacagcgcga atgcgaagcc ggagtattcg
    18661 acgtggtgtc cggcaacgat ttcagactca ccctctgcca cgtcaaacgg cgcgcggttg
    18721 gtttcggcaa cggcggaaat cagatagacg ataaagatgg ggaagagcgg cagccagttc
    18781 caagagaata ccgaaccgcc ggcaatacct ttggcttgtg cggcaacgat gtcggagaag
    18841 ttcatgctgc ccgataccat cacgacacac accagcgcgg cactcatggc aatctcgtag
    18901 gaaatgcttt gtgcggaagc acgcattgcg cccaaaaacg aatatttgga gttggaagcc
    18961 cagcccgcga tgatcacgcc gtaaaccgac agcgaggtaa tcatcaggat gtacaaaaga
    19021 ccgatattga tgttggtcag cacccattct tcgttaaacg gaatcaccgc ccacgccgca
    19081 aaagacgggg cgagcgacat aatcggacca atgtagaaca gggctttgtt tgacagcctc
    19141 ggacgggtta cttctttaaa caagagtttg aacacgtcgg caaacggctg aatcagaccc
    19201 cacggaccgg ttacgttcgg accgacgcga agctgcatga agccgatgac tttacgttca
    19261 aaataagtca ggtaggcgac ggtcagaatc agcggaatca ggataatcac aattttgacg
    19321 atgacggata ccaccaagcc tacagtaata cccaaatcgc ccagaccgag cgttgcggca
    19381 aagaggtttt ggaaccattc ttgcatgatc aagctcccgc cagttcaata gtgtccatca
    19441 acgcacccag cgcggcattt tcggtatgca gcggcaaatg caccacgttt tcaggcaaac
    19501 cggcatcggc tttgacggca accgatacgc ttgcgccgtt ttgtttggcg acagcggttt
    19561 gtccgtcttg caggcccaag cgtgccaatg tatttggatt tacacgcgca gcaggcacgg
    19621 cggcatggct ggtttcttgc aacggtgcgg aacggcgcac gatagcatcg gtgtgataga
    19681 tgcccactcc gcccacgcgc acgaggcggc ttgatgccgt ctgaaaatct ttttgagtcg
    19741 aagacacgcg gttgtccagt ttggacggca ggctttccgc gtccagcgcg tctttcagaa
    19801 tcgcggcggt atcgtggtat tcaaaacctt gcaggtcaaa caggttgccc aatacgcgca
    19861 acactttcca cagcggacgc gagtcgccga agccttgtac cacgccgtga aaggattgca
    19921 gacggccttc catattgatg aagctgcccg atgtttcggt aaacggcgca atcggcagca
    19981 atacgtcgca cacgtccagc agcgtttcgc tgacaaacgg tgtaaacgcc atcacgcttt
    20041 tcgcctgttt caacgcggct acggcttttg caccgtcaac cgtatcgatt tcaggctcga
    20101 cgttgagcaa caataccgcc tgtttcggcg cgtttgccat ttcggcaacg ctctcgccgg
    20161 agttcacgcc caaaacgtcc gcaccgacgc tgttggcggc ttgcggcaga atgcccagca
    20221 ctgcaccggt cgcgtctgcc agctcttgcg cagcggcata aatggcggcg taatcaggat
    20281 ggttttgtac ttccgcaccc aaaatcaccg ctgctttttc agcgtttttc aggctggcgg
    20341 taacggcgtg ttccgcatcg gcagacaggt ttttcagacg acctgcccac tcgtcgggat
    20401 gtacggcttc ttgagacaga agcggcataa acaattcttc cttgctgccg gccaatacgc
    20461 tcaaggccat acggtctttg gcggcgcggc gcaggcgggc ggtcaggagc ggctgttctt
    20521 tgcgcaagtt cgcgccgact accaatacgg catcgttgtc agccaaagat tcaatgcttt
    20581 gtcccaacca ttgcgcaccc ttaatgccgt ctgaaagacg tttgtcttgt tgacgcaaac
    20641 gggttgcaaa gtttttaaca cccaagccgt cggcaagttt tttggccaga tacagctctt
    20701 caaccgtatt catcgggttc gcccaaatgc cgacttggtt ttggttgccg tctttggcga
    20761 tacattcaat cgcgctgcgg acatattcca atgcggtttt ccaatccacg tccatccact
    20821 cgccgccctg tttgattttc gggtttttca gacggctttc gtgatacagg ccttcgtagg
    20881 caaaacggtc gcggtcggac aaccagcatt cgttaatcgc ttcgttttcc agcggcagta
    20941 cgcggcggac ggtatggtct ttggtttgta caatcaggtt actgcccaaa gcgtcgtggg
    21001 cggaaacgga tttgcggcgg ttcaattccc aagtacgcgc attgaagcgg aacggtttgc
    21061 tggtcaatgc gccgacagga cacaaatcga taacgttgcc cgacaactcg gtttccaccg
    21121 ctttaccaat gaaaggcatg atttcggagt gttcaccgcg attcgccatc gcaatttcct
    21181 gcaaaccggc gatttcttcg gtgaatcgca cgcagcgggt acagtggatg cagcggctca
    21241 tttcctcggc ggaaaccaaa ggccccatat ctttgccgac gacggaacgt ttttcttcgg
    21301 tatagcggct ggtggttttg ccgtagccca ccgccaaatc ctgcaactgg cattcgccgc
    21361 cttggtcgca ggtcggacaa tcaagcggat ggttgatgag caggaactcc attacgcctt
    21421 cttgcgcctc tcgggctttt gccgaatgcg tgcgcacaat catgccgtct gtaaccggcg
    21481 tggcacaggc tggcagaggt ttgggggctt tttctacgtc caccagacac atacggcagt
    21541 tggcggcgat ggaaagtttt ttgtggtaac agaaatgcgg aatataagta ccgagcttgt
    21601 gcgcggcttc aatcaccgtc gcgccctgct ccacagatac ttgtttgccg tcgatttcga
    21661 tttgtaacat ggttcgttcc tagttacggg tatttgataa ataatcttat ggaggtcgtc
    21721 tgaaaaatgg gtttcagacg gccttttgag tttcattgac aatcaatact gtttaaaacg
    21781 tttactgcca ccttgccgtc attcccgcgt aggcgggaat ccattttttg aatttcggca
    21841 actgcttttc aaatatcggt ttctttagat tccactatgg attcccgcct acgcgggaat
    21901 gacggcaaag ttaaattttt agcattttgc ttttaaccaa tataaaacca actaaaacta
    21961 taacttattt attatatttt taaatgactt aaataactct ttaacagcac gctcattgtc
    22021 ttcatctgta ggctcaaact tttcacacca attaattata tttacatcgc catgcaataa
    22081 ctctgcataa catcctactg catcgcaact accaccccgt gtccttgacg tgccaaaaat
    22141 aaaatcacaa ttatgcgaat aaagtttgta taaatttctc ctaacctcgt attcattatc
    22201 accagatgaa taaaaaccaa ttctctgacc ttcatgatca aatactgcaa caaaatctaa
    22261 gccgtttcca tgtctttcaa aaaagactag aaatttagaa aatttccgac aaatctgatt
    22321 aaaaagcgta ttgagtgtcg tactcttacc tttgttggct gcaccgtata gaataaaaat
    22381 tttagcttcc accattacca ccctccccac ttatgctctt tcatcggtcc gccgtgttcg
    22441 atgtaatgca caaactcatc acggaaatgc ttggtaaagc tgcggacagg gaagacggcg
    22501 gcatcggcga gggcgcagat ggtgcggcct gccatttggt tgccgacgga atccagcaaa
    22561 tccaaatctt ccattttacc tttgccttcc acgatacggt ggacgatgcg gtagagccag
    22621 cccgtacctt ctcggcaagg cgtacattgg ccgcaagact cgtcgtagta gaagtagctc
    22681 aggcgctcaa gggctttcac catgcacacg tcttcgtcca tgacgataat cgcgccagaa
    22741 cccagcatcg agcctgcttt ggaaatcgag tcgtagtcca tatttgtctg catcatgatg
    22801 tcggcaggca atacgggcgc ggacgaaccg ccaggaataa cggccttgag ttttttgccg
    22861 ccgcgcatac cgcctgccat tttcaagact tcggcaaacg gtgtgcccaa tggcacttca
    22921 tagttgcccg gacgctcgac atggccggaa atacagaata atttggtacc gcctgcattc
    22981 ggaatacctt tatcggcaaa tgcctgtccg ccgtcacgga taatgaatga aacggaggag
    23041 aaagtttcgg tattgttgat ggtagtcggt ttgccgtaca ggccgaacga agcagggaat
    23101 ggcggtttaa agcgcggctg gccttttttg ccttccagcg attcaagcaa tgcggtttct
    23161 tcgccgcaga tgtatgcacc gtagccgtgg tgggcgaaga gttcgaattc aaaatccgaa
    23221 cccaaaatat ttttacccaa aaagcctgcg gcacgcgcct gctccaaagc ggcctcaaag
    23281 cgttggtagc cttcaaaaat ttcgccgtgg atatagttgt aaccggcttt cgcgcccatc
    23341 gcgtaaccgg cgataatcat gccttcaatc agggcatggg ggttgaacat aataatgtcg
    23401 cggtctttaa atgtacccgg ctcgccttcg tcggtgttgc aaacgacgta cttttcgccg
    23461 gggaaagaac ggggcataaa gctccatttc aaaccggtcg ggaagcccgc accgccacga
    23521 ccgcgcaagc cggaggtttt gacttcgtca atcacatcgg tttgcgagat gttttcggac
    23581 aggattttac gcagggcggt ataaccgcca cgtttgacgt attcgtccaa tgtccagcaa
    23641 tcgggattgg cggtatccac ttggtcaaaa atcacgcctg attggtaaat agccattttt
    23701 ggtgtgcctg tttgttttcg tatcggttgc ggccgctgtt tcagacgacc ttaagatgtc
    23761 tttgtgtacc ggcttgtaac gtcgtctgaa ataaaatcta gtttatcaaa tcgatatttt
    23821 cagacgacct ttaatcgttt ttgttggatc tcgaccactt gcttgtctgt cgtcattccc
    23881 gcgcaggcgg gaatccatcc tcaatggtaa gcaatgtctt attaaattca gaaaccgaat
    23941 cttaccggtg gattcccgcc tgcgcgggaa tgacggcatt tcggtatttc agtagggcgg
    24001 attcttaaat ccgacatttt gcctttttac ccactctgtc ggctacaagt atccgaccta
    24061 cgtttaaatc gtcgtttcag acgacctact ccaactccgc cagtttcttc tcaatcgctt
    24121 cttcggtcat aaagctgcac atgctgtggt tgttgaccag cataacggga gcgtcgccgc
    24181 atgcgcccat gcattcgcct tcgacaaggg taaatttgcc gtcgggcgtg gtttcgccgt
    24241 agccgatacc gagtctttgt ttgaggtatt cgccggtcgc cataccgccg cgcagggcgc
    24301 agggcaggtt ggtacaaacg gtcagtttgt atttgccgac aggctcaaga tcgtacatat
    24361 tgtagaaggt agcgacttcg taggcttgtg caggcgtgat gccgatatag tcggcaacaa
    24421 aagcgatggt ttcgggggcg agccagcctt tttcggtttg ggcgatacgc aacgcaccca
    24481 tgatggcgga acggcgttgg tcggcgggat atttcgccag ttctgtgtcg atttgtttta
    24541 aggactctgc ggataacatt atcggtcaac ctccccgaat acgatgtcct gcgtaccgat
    24601 gatggcaacg acgtcggcga gcatgtggcc ttttgccatt tcgtccatgc cttgcagatg
    24661 ggcgaagccg ggtgcgcgga ttttcaggcg gtagggtttg tttgcgccgt ctgaaatgat
    24721 gtaaacgccg aactcgcctt tcggatgttc gacagcggtg taggtctcgc cctcgggaac
    24781 gtgcataccc tcggtaaaga gtttgaaatg gtgaatcagg tcttccatac ctgttttcat
    24841 ttcggtacgt ttgggcggag cgaatttgtg gtttgtggta atgaccggac ccggattgac
    24901 acgcaaccac tcggaacatt gtttgatgat gcgtacggat tgacgcattt cttccatacg
    24961 gcagaggtag cggtcgtagc agtcgccgtt cacgccgaca gggatgtcga aatccatttt
    25021 gtcgtacact tcgtaaggct gtgtcttacg cacgtcccat tccacgcccg aaccgcgcaa
    25081 catcacgccg gtaaagcctt tttgcatggc acgctcggga gagacaacgc cgatgccgac
    25141 ggtacgctgt ttccagatgc ggttgtcgtt caggagggtt tcgagtgtgt cgatattttt
    25201 ggggaagcgt tcgcagaagg catcgataaa gtcgagcatg gtgccttcgc gggattcgtt
    25261 gagctgcttc aatactttgg cattgcggaa tttgctgctc tcgtatttag gcatgaagtc
    25321 gggcaggtcg cggtaaacgc cgccgggacg gaagtaggct gcatgcatac gtgcgccgga
    25381 cacggcttcg tacaagtcca tcagctcttc gcggtcgcgg aaggcgtaga ggatggcggt
    25441 catcgcgccg atgtcgaagg catgcgaacc gatgcccatc aagtgattga ggatgcgcgt
    25501 tacttcggca aacatcacgc ggatgtattg ggcgcggata ggcacgtcaa ttccggcgag
    25561 cttttctact gccaaacaat acgcctgctc gttgaccatc atggaaacgt aatccaagcg
    25621 gtccatatag ggcaaggctt gcagataggt tttggtttcc gccagtttct ctgtgcctcg
    25681 gtgcaggagg ccgatatgcg ggtcggcacg gacgatggtt tcgccgtcca gctccaagat
    25741 catacgcaat acgccgtgcg ccgcagggtg ttgcgggccg aagttgatgg tgtagtttct
    25801 taatttattg gccaccgtag ttctcctcac ggacgatacg cggcgtgatc tcgcgcggct
    25861 caatggtaac aggttggtaa atcacgcgtt tttgctcttc gtcgtaacgc atttccacat
    25921 agccggaaat cgggaagtct ttgcggaacg gatgtccaac gaaaccgtaa tcggtcagga
    25981 tgcggcgcaa gtccgggtgg ttgttaaaca taatgccgta catatcgaag gcttcgcgtt
    26041 cgtaccaatc cgcactgttg taaatatcga ctacggattc gactacgggg aagttgtcgt
    26101 ctgaaaccca gacgcgcacg cggatgcgct gattgttttt aacggaaagc aactgactga
    26161 cgacggcaaa gcgtttgccc tgccatactt cgtttttgta agtgctgtaa tcgacaccgc
    26221 acaagtcgac cagaagctcg aaatgcaact cttcatggtc gcgcaatgcg gtcatgactg
    26281 aaatatagtg ttcgggcaga cactcgacgg taatctcacc caaagcggaa atgactttgc
    26341 ctgcctgatt gcccaaaacg cggctgacgg tttcgtataa gttttgaatg cttgccatat
    26401 cgtcctctcc ttactcgtca cgcgcgatgg tggaagtgcg cttgattttt tgttggagct
    26461 gaatcagacc gtaaatcagg gcttccgcag tcggcggaca acccggcaca taaacgtcta
    26521 ccggcacgac gcggtcggca ccgcgcacaa cggaataaga atagtgataa taaccgccgc
    26581 cgttggcaca cgagcccata gacaataccc agcgtggctc ggcgagctgg tcgtatacgc
    26641 ggcgcagggc gggcgccatt ttgttggtca gcgtacccgc cacaatcatc aggtcggcct
    26701 gacgtgggga cggacggaaa atgataccga aacggtcaag gtcataacgc gccatacccg
    26761 catgcatcat ttccacggcg cagcaggcca agccgaaagt aaccggccac aacgaaccgg
    26821 tacgcatata gttcaacacc gtatccgcgc tggtggtgat gaaacctttt ttcaaaacgc
    26881 cttctattcc cattccagcg cacctttttt ccattcgtaa acaaagccta ccgtcagaac
    26941 gacgataaac accaacatag accagaagcc gtacgcgccc aaatctttga acacaactgc
    27001 ccacggcaac atgaacgcga cctccaaatc aaacaggatg aagaggatgg cgacgaggta
    27061 atagcgcacg tcaaacttca tcctcgcatt ttcaaaggct tcgaaaccgc attcgtaagg
    27121 cgcgtctttt tcggcatagt ggcgtttcgg gcctaaaatc gtgccgagca ggataaacag
    27181 tacgccggcc gcgaggccga cgaggataaa gacaaagacg ggaaagtaag cggacaacat
    27241 ggttacaccc aaatccgtta acaaaatttc tacaataatt tcgtatttta gcgaatttca
    27301 aaaaccatta aaaggtaaat atcggcaaaa cgcccaaaaa aacccaataa atacaatcat
    27361 gttatgataa cgattcttat ttgattttat aaggtcatat aaatttttac accgtaatt
//
LOCUS       DAXHKX010000007        26554 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHKX010000007 DAXHKX010000000
VERSION     DAXHKX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26554
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.007"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            165..788
                     /gene="lolA"
                     /locus_tag="ACFMWZ_000291"
     CDS             165..788
                     /gene="lolA"
                     /locus_tag="ACFMWZ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC5934315.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1061..2200
                     /locus_tag="ACFMWZ_000292"
     CDS             1061..2200
                     /locus_tag="ACFMWZ_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC5934316.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTGQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRSDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEETANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLLPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2334..2600
                     /locus_tag="ACFMWZ_000293"
     CDS             2334..2600
                     /locus_tag="ACFMWZ_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC5934317.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKISNTQHPTPDTEHQTSNIYIRITKGRQKLGIPE"
     gene            2552..3331
                     /locus_tag="ACFMWZ_000294"
     CDS             2552..3331
                     /locus_tag="ACFMWZ_000294"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC5934318.1"
                     /translation="MYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINCQVLLDFLSK
                     DDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFRKFPGSACNK
                     IIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDSKTGTV
                     SEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIECDADIKRRA
                     YHLRFMLLKSNKLIYKMIFPIITLLGVDITGRILKVIRGNV"
     gene            3372..3902
                     /locus_tag="ACFMWZ_000295"
     CDS             3372..3902
                     /locus_tag="ACFMWZ_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC5934319.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLIIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(3936..4052)
                     /locus_tag="ACFMWZ_000296"
     CDS             complement(3936..4052)
                     /locus_tag="ACFMWZ_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC5934320.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(4277..5872)
                     /locus_tag="ACFMWZ_000297"
     CDS             complement(4277..5872)
                     /locus_tag="ACFMWZ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC5934321.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(5979..6374)
                     /gene="hisI"
                     /locus_tag="ACFMWZ_000298"
     CDS             complement(5979..6374)
                     /gene="hisI"
                     /locus_tag="ACFMWZ_000298"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC5934322.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(6405..7172)
                     /gene="hisF"
                     /locus_tag="ACFMWZ_000299"
     CDS             complement(6405..7172)
                     /gene="hisF"
                     /locus_tag="ACFMWZ_000299"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC5934323.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPGNTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(7185..7922)
                     /gene="hisA"
                     /locus_tag="ACFMWZ_000300"
     CDS             complement(7185..7922)
                     /gene="hisA"
                     /locus_tag="ACFMWZ_000300"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC5934324.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(7955..8593)
                     /gene="hisH"
                     /locus_tag="ACFMWZ_000301"
     CDS             complement(7955..8593)
                     /gene="hisH"
                     /locus_tag="ACFMWZ_000301"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC5934325.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALKRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDRQGCRLKVPHMGWNTVRQTQNHPLFKDIPQNTRFYFVHSYY
                     FDPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(8724..10226)
                     /gene="pta"
                     /locus_tag="ACFMWZ_000302"
     CDS             complement(8724..10226)
                     /gene="pta"
                     /locus_tag="ACFMWZ_000302"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC5934326.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRNANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(10411..11469)
                     /gene="fbpC"
                     /locus_tag="ACFMWZ_000303"
     CDS             complement(10411..11469)
                     /gene="fbpC"
                     /locus_tag="ACFMWZ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC5934327.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSDAPAGTHGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(11490..13007)
                     /gene="fbpB"
                     /locus_tag="ACFMWZ_000304"
     CDS             complement(11490..13007)
                     /gene="fbpB"
                     /locus_tag="ACFMWZ_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC5934328.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(13075..14070)
                     /gene="fbpA"
                     /locus_tag="ACFMWZ_000305"
     CDS             complement(13075..14070)
                     /gene="fbpA"
                     /locus_tag="ACFMWZ_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC5934329.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(14321..14791)
                     /locus_tag="ACFMWZ_000306"
     CDS             complement(14321..14791)
                     /locus_tag="ACFMWZ_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFC5934330.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFERHDGKLESMDGREQELVLIW
                     RAEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(14849..16225)
                     /gene="argH"
                     /locus_tag="ACFMWZ_000307"
     CDS             complement(14849..16225)
                     /gene="argH"
                     /locus_tag="ACFMWZ_000307"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC5934331.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELSLEVLQGFSKLISDDVYSVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(16244..17113)
                     /gene="galU"
                     /locus_tag="ACFMWZ_000308"
     CDS             complement(16244..17113)
                     /gene="galU"
                     /locus_tag="ACFMWZ_000308"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC5934332.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMIEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(17141..17740)
                     /gene="rdgB"
                     /locus_tag="ACFMWZ_000309"
     CDS             complement(17141..17740)
                     /gene="rdgB"
                     /locus_tag="ACFMWZ_000309"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC5934333.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(17733..17966)
                     /locus_tag="ACFMWZ_000310"
     CDS             complement(17733..17966)
                     /locus_tag="ACFMWZ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC5934334.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(18074..18607)
                     /locus_tag="ACFMWZ_000311"
     CDS             complement(18074..18607)
                     /locus_tag="ACFMWZ_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC5934335.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(18708..19166)
                     /gene="nudB"
                     /locus_tag="ACFMWZ_000312"
     CDS             complement(18708..19166)
                     /gene="nudB"
                     /locus_tag="ACFMWZ_000312"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC5934336.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFDMEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            19377..19453
                     /locus_tag="ACFMWZ_000313"
     tRNA            19377..19453
                     /locus_tag="ACFMWZ_000313"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19411..19413,aa:Pro,seq:ggg)
     gene            19611..21437
                     /gene="mafB"
                     /locus_tag="ACFMWZ_000314"
     CDS             19611..21437
                     /gene="mafB"
                     /locus_tag="ACFMWZ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC5934337.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            21440..21904
                     /locus_tag="ACFMWZ_000315"
     CDS             21440..21904
                     /locus_tag="ACFMWZ_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934338.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            22129..22275
                     /locus_tag="ACFMWZ_000316"
     CDS             22129..22275
                     /locus_tag="ACFMWZ_000316"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934339.1"
                     /translation="MWYEANIGLINTMSRSNQAGTRLLYSNYGLLYITTDHYISATRF
                     VAWK"
     gene            22287..22574
                     /locus_tag="ACFMWZ_000317"
     CDS             22287..22574
                     /locus_tag="ACFMWZ_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFC5934340.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            23088..24332
                     /locus_tag="ACFMWZ_000318"
     CDS             23088..24332
                     /locus_tag="ACFMWZ_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC5934341.1"
                     /translation="MIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEALKKDKQ
                     TARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKESLYYLN
                     KHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGSCAETFG
                     LGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEYVQFSID
                     LFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAH
                     AQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVE
                     IKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSK
                     SGVEIEGFTEPNRTAYPLYNSK"
     gene            24359..24778
                     /gene="mafI"
                     /locus_tag="ACFMWZ_000319"
     CDS             24359..24778
                     /gene="mafI"
                     /locus_tag="ACFMWZ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC5934342.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            24868..24939
                     /locus_tag="ACFMWZ_000320"
                     /pseudo
     CDS             24868..24939
                     /locus_tag="ACFMWZ_000320"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            24936..25178
                     /gene="imm31"
                     /locus_tag="ACFMWZ_000321"
     CDS             24936..25178
                     /gene="imm31"
                     /locus_tag="ACFMWZ_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC5934343.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            25256..25501
                     /locus_tag="ACFMWZ_000322"
     CDS             25256..25501
                     /locus_tag="ACFMWZ_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC5934344.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            25504..25893
                     /locus_tag="ACFMWZ_000323"
     CDS             25504..25893
                     /locus_tag="ACFMWZ_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934345.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            25994..26434
                     /locus_tag="ACFMWZ_000324"
     CDS             25994..26434
                     /locus_tag="ACFMWZ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934346.1"
                     /translation="MWKITKEQGQDLSFATYCLLSAAINLQEFKLWLEKVVLDMPIDN
                     IHFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPETFAKFLSLPTAETQTQVFGCFRYQSLPNSTQIPP"
BASE COUNT         6507 a         6111 c         7359 g         6577 t
ORIGIN      
        1 ggcacacatt aaagggcagc gtggcgcgcc gccttttccg gtgggcaaaa aatcagccct
       61 cggaaaacgc ggtttgcaaa atgcaaaccg accgtaacgc cacccgtatg attgttttgc
      121 cgcgccgata ctttacgcca cacccatccc gacaaggaaa aataatgatg aaaccgcaca
      181 acctgttcca attcctcgcc gtttgctccc tgaccgtctc cgtcgcttcc gcacaggcgg
      241 gcgcggtgga cgcgctcaag caattcaaca acgatgccga cggtatcagc ggcagcttca
      301 cccaaaccgt ccaaagcaaa aagaaaaccc aaaccgcgca cggcacgttc aaaatcctgc
      361 gcccgggcct ctttaaatgg gaatacactt cgccttacaa acagactatt gtcggcgacg
      421 gtcaaaccgt ttggctctac gatgtcgatt tggcacaagt gaccaagtcg tcccaagacc
      481 aggccatagg cggcagcccc gccgccatcc tgtcgaacaa aaccgccctc gaaagcagct
      541 acacgctgaa agaggacggt tcgtccaacg gcatcgatta tgtgctggca acgcccaaac
      601 gcaacaacgc cggctaccaa tacatccgca tcggcttcaa aggcggcaac ctcgccgcca
      661 tgcagcttaa agacagcttc ggcaaccaaa cctccatcag tttcggcggt ttgaatacca
      721 atccccaact ctcgcgcggc gcgttcaagt ttaccccgcc caaaggcgtg gacgtgttga
      781 gcaactgatg ccgtccgccc cgatgccgtc tgaaagccgc cgaggcttca gacggcattt
      841 tcacgcaggc ggaacaatgt cccgcattac cgcccgatcg ggcatcggaa cggcaaaccc
      901 gtgaaaatta acggttgcgc cccggttgtt tttgccgttt aatgcaaacc ttgctgcatc
      961 aagggccaag aaagccgacc ggccgccccc acagcttccg atgcaggcgg ccaggccgtc
     1021 cctgcaatgt tttttatttt tgaacgaaag gtcgaaaacc atgaaaaaaa cactggtggc
     1081 ggcggcaatc ctgagcctcg ccttgactgc gtgcggcggc ggaagcgata ccgccgccca
     1141 agccccctcc gccaagcctg aagccgaaca atcgggcaaa ctcaatatct acaactggtc
     1201 ggattatgtc gatcccgaaa ccgtcgccgc ctttgaaaaa gaaaccggca tcaagacgcg
     1261 ttccgattat tacgacagca acgaaacact ggaggcaaaa gtcctgaccg gcaaatccgg
     1321 ctacgacctg accgcgccgt ccatcgccaa cgtcggccgg caaatcaaag cgggcgcgta
     1381 tcagaaaatc gacaaggcgc aaatccccca ttacggcaac atcgataaag atttgctgaa
     1441 aatgatggaa gccgtcgatc cgggcaacga atacgccgtc ccctatttct ggggcatcaa
     1501 caccttggca atcaacacgg ggcaggtgca aaaggcattg ggtacggaca agctgcccga
     1561 aaacgaatgg gatttggtgt tcaaacccga atacaccgcc aaactcaaat cctgcggcat
     1621 cagctatttc gacagcgcaa tcgaacagat tcccttggcg ttgcactatt tgggcaaaga
     1681 ccccaacagt gagaatcccg aagacatcaa agccgccgtc gatatgatga aagccgtccg
     1741 gagcgacgtg aaacgcttca gctcttccgg ctatatcgac gatatggcgg cgggcaacct
     1801 gtgtgccgcc atcggttacg gcggcgattt gaacattgcc aaaacccgtg ccgaagaaac
     1861 ggcaaacggc gtggaaatca aagtattgac cccgaaaacc ggcgtgggcg tgtgggtgga
     1921 ttcctttatg attccgcgcg acgcgcaaaa cgtcgccaac gcccaccgct atatcgacta
     1981 cacgctcctg cccgaggtgg cggcgaaaaa cggcagcttc gttacctacg cacccgccag
     2041 ccgccccgcg cgcgagctga tggatgaaaa atacacctcc gacgcatcga ttttcccgaa
     2101 caaagaactg atggaaaaaa gtttcatcgt atcgcccaaa tccgcagaat ccgccaaact
     2161 gggcgtgaag ctgtggcaag ggctcaaagc gggcaaataa ccggaatccc tgccgtctga
     2221 aacctttcgg gcggcaggaa acggcgcgtc cgttatcaaa cagggggggc gtttcccctc
     2281 ctgccggtta tgattgggtt aagattaaaa tgatttagta aaatgagaaa gatatggatt
     2341 taagtatcgt agttcccatt tataatgtcg aaagttattt ggaagcgtgt ttaagttcca
     2401 tagaacctat attaagtaat gaaaatgtcg aacttatcct tgtgaatgac gggtcaaaag
     2461 acggaagtga agatatatgt tataaatata tagataaaat atcaaacacc caacacccaa
     2521 caccagatac cgaacaccaa acatcaaata tatatatcag gataaccaag ggacggcaga
     2581 agctaggaat accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcgga
     2641 tgattttatt aattgtcagg tcttgctgga ttttcttagt aaagatgata ttgatatgcc
     2701 ggatgtggtt tttttaaatg cggttaaata tgataaggga agtgtttcac attttggcga
     2761 agattatcag cctgaaaaaa tactcaatca atccaaagtc gaagttttga aaggattatg
     2821 ccgatttaga aaatttccgg gttcggcgtg taataagatt ataaaaaggg aattgattat
     2881 caaagaaaaa ctgttttttg aaaaagaggt ttatgccgaa gatatcgaat ggtcaatgag
     2941 attatttaat gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca
     3001 gggaagaaaa gattctaaaa caggaactgt ttcggaaaaa agtataaagt cattattata
     3061 tattttggag aaaaatgcgg aaatggaatt taatagggat atatcaagtt atctttattc
     3121 ctttctttcc tacgaatatc tcgttttgct ttttatcatg acgagtaaaa atatagagtg
     3181 tgatgctgat ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa
     3241 gttgatatat aagatgatat tcccgataat cacattactc ggggtcgata ttacaggcag
     3301 aattttaaaa gtaatcagag ggaatgttta ataaatcctt taacaatata tatcttaccg
     3361 aaggaggaaa aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa
     3421 gctgtatgat agacgaagcg tgcgtcgtca tcggcgaagt gtcgcttgcc gaagacgttt
     3481 ccgtgtggcc gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca
     3541 gcaatataca ggacggcagc gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag
     3601 ggtcgccgct gattatcggc gaagatgtta ccgtggggca caaagtgatg ctgcacggct
     3661 gccgtatcgg caaccgcgtc ctggtcggca tggggacgac ggttctggac gatgccgtga
     3721 ttgaggacga agtgatgatc ggcgcgggta gcctcgtgcc tccgcgtaaa cgtttggagg
     3781 gtggctatct ttatgtcggt tcgccggtca gacaagtgcg cgtgctgacc gatgaggaaa
     3841 aagccttttt gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt
     3901 gaaatcacat cggcgttctt gcgtcagccc caaattcatg cggatgggac gcatccgata
     3961 acggtatccg atgcgccttg attttgaccg tctgcgtttg agttgcaggc aaaaatgccg
     4021 tctgaaagcc ttttttcggg ttcagacggc attttatcgc cgattgcttt ttacagtttg
     4081 accgaatgtt cgcgcgtttc gtggaacacg atgtccggcc aacgttcttg cgtcagccct
     4141 aaattcatgc ggacgggatg cccgataacg gtatccgatg cgccttgatt ttgatcggtg
     4201 catttgagtt gcaggcaaaa atgccgtctg aagccctttt ttcgggttca gacggcattt
     4261 tattgccgat tgctttttaa agtttgaccg aatgctcgcg cgtttcgtgg aacacgatgt
     4321 ccggccagcg ttcttgcgtg agtcccagat tcacgcggtt aggggcgagg taggcgaggt
     4381 tgccgcccgc gtcgatggcg aggttgcctg cattggcttt ttcaaattcc gccagttttt
     4441 tcttgtcgtc gcacgatacc cagcgcgccg accagatgga tgcgctgtcg aacacggctt
     4501 ctacgccgta ttcgttggcc agccgcgagg taacgacttc aaattgcaac acgccgaccg
     4561 cgcccaaaat caaatccgcg ccgctcatcg gtttgaacac ctgcaccgca ccttcttcgc
     4621 caagctgttg caaacctttt tgcagttgct tgattttcag cgggtttttg atgcgaacgc
     4681 tgcggaacag ttcgggcgcg aagaatggga tgccggtaaa cgtcagttgt tcgccttcgg
     4741 agaagctgtc gccgatttgg atgttgccgt ggtttgggat accgataatg tcgccggcgt
     4801 aggcttcttc aaccagctcg cggtcgtggg acatgaaggt taccacgctg gaggcggcga
     4861 tttcgcggtt gatacgcagg tgtttcattt tcatgccgcg ctcgaattta ccggagcaga
     4921 cgcgcaaaaa agcgatgcgg tcgcggtgtt tcggatccat attggcttgg attttaaaga
     4981 taaatccgga aaacttagcc tcgtccggct cgaccatgcg tacggtcgcg tcgcgcggtt
     5041 tcggtgcggg tgcccagtca atcaatgaat tgaggatttc ctgaataccg aagttgttaa
     5101 tcgcagagcc gaagaacacg ggcgtgagtt cgccggcgag aaattcgtcg agattgaact
     5161 cgttcgatgc tgcctgcacc aattcgattt cgtcgcgcaa ctgttggatt tccaatggaa
     5221 agcgttgttc caattcggga ttgtctatgc ctttgatgat gtcgaactcg tgcggcaggc
     5281 gttcgccgcc cgcttcaaag agatagattt cgtcgttcag gatgtggtac acgcctttga
     5341 agtttttgcc cataccgatt ggccaggtga cgggcgcgca gcggatttgc aggatgtttt
     5401 ccacttcgtc cagcaattcc agagaatcgc gtacttctcg gtcgtatttg ttcatgaacg
     5461 taacaatcgg cgtatcgcgc aggcggcaga cgtttaagag cttgatggtt tgcgcttcca
     5521 cgccttttgc cgcgtcgatg accatcaacg cgctgtccac ggcggtcaaa acgcggtagg
     5581 tgtcttctga gaaatcttgg tgtcccggcg tgtccaagag gttgacggtg tggtctttat
     5641 agtcgaactg catcacgctt gatgccacgg aaatgccgcg ctgcttctcg atgtccatcc
     5701 agtcggaagt ggcgaatttg ccggtttttt tgccttttac cgtacccgcg ctttgaatcg
     5761 cacctgaaaa cagcaagagt ttttcagtca acgtggtttt acctgcgtca gggtgggaga
     5821 tgatggcaaa cgtgcggcgg cggcgcactt ggtcgaggat ttcttgggac atggttttct
     5881 ttgcaaaaag gttcaggccg cttttcagac ggcccggaca gtgtttgaga cggcgaaatt
     5941 gtacaaaaaa atgcctgata attcaatgtt ggaggcggtc agtgcgtgct gccgtaaatc
     6001 tctttttcgt ctttcaggac ggcatcggcg gtttcccacg cgctgccacg ccagactttg
     6061 taaaagcagc tttctcgccc ggtgtggcag gcgatgccgc cgttttgggc gatgagcatc
     6121 acaatggcgt cgccgtcgca gtcgaggcgc agttcgcgga ctttttgcgt gtgtcccgac
     6181 tcttcgccct tcatccattg tttttggcgc gaacggctgt aatagtgggc aaagccggtt
     6241 tcgacggttt tttgcagggc ttcggcgttc atccacgcca ccattaaaac acgtttggtt
     6301 tcggcatctt gggcgatggc gcaaaccaaa cctttttcgt caaatttgac ggcttcaagc
     6361 aggtttttat ccatattttc tttcagacgg catggtcggg gcggtcagag gcgcacttcg
     6421 atgccggctt cgcgcatagc gcgtttggct tcgcggatgg cgatttcccc gaaatggaaa
     6481 acgccggcgg caagtacggc atcggctttg ccttcggtta tgccttcaat caggtgccgg
     6541 acattgccga ccccgccgga ggcgatgacg gggatgtcga cggcttcggc aacggcgcgg
     6601 gtcagcggca ggttgaaacc ctgtttcgta ccgtccctgt ccataccggt aagcaggatt
     6661 tcgcccgcgc cgcgtttttg catttcgatc gcccattcca ccgcatccaa accggtcgga
     6721 ttgcgcccgc cgtgggtaaa gatttcccag cgtgtgtttc cggggttgac ggctttggca
     6781 tcgacggcgg cgacgatggc ttgcgaaccg aaaaatccgg cggcttcgtc aatcaggtcg
     6841 ggacgggtaa cggcggcggt gttgatgctg actttgtccg cgccggcatt gagcaggcgg
     6901 cggatgtcgg caacggtgcg tacgccgccg ccgacggtca gggggatgaa gacttgtccg
     6961 gcaacttctt cgatgatgtg caggatggtg tcgcggttgt cggatgaggc ggtgatgtcg
     7021 aggaaggtca attcgtccgc gccttcgccg ttgtagcgtt tggcggcttc gacggggtcg
     7081 cccgcgtcgc gcaaaccgat gaagttcacg cctttgacga cgcgcccgtc ttttacgtcg
     7141 agacagggga tgatgcgttt tgccagtgcc ataatcggat gcctttagtc gagggaatct
     7201 gccagttgct gcgcttgggc aaaatcgatg ctgccctcgt aaatcgcgcg gccggtaatc
     7261 gcgcctgcta cgccatgttt ttcggcggca cacagggcgc ggatgtcgtc caagccggtc
     7321 agtccgccgg aggcgatgac gggaatgcgg acggcttggg cgagtttgac cgtcgcgtcg
     7381 atgttcacgc cgctcatcat accgtcgcgc ccgatgtcgg tgtagatgat gctgttgacg
     7441 ccgtcgtctt caaagcgttt tgccaaatca attacatgat gcccggttac ggttgcccag
     7501 ccgtcgatgg cggccatacc gtctttggca tccagcccga cgataatcct gccggggaag
     7561 gctttgcacg cctcgcgcac gaagtcgggg tttttgactg ccgccgtgcc gataatcacg
     7621 tcgtttaagc ccaaatccaa atattgtccg atggttttca aatcgcgtat gccgccgccg
     7681 agctgtacgg ggatgtcttt ggcgacggcg gcgaggatgt ctttgatggc gggcaggttt
     7741 tgcggaacgc cggcaaacgc gccgttcaaa tccaccagat gcaggcggcg cgcgccttgt
     7801 ttgaaccagt gcagtgcggt ttcggcgggc gaatcggaaa agacggtcgc ctcttccatc
     7861 agcccttgtc tcaggcggac gcagcgtcct tctttcaaat cgatggcggg tatcagcagc
     7921 atagtttttt tccttgtgcg gggccgtgtc cggcttacca gtttaaaaag tttttcaaca
     7981 tcgtcagccc ggcatcgtgg cttttttcgg tgtgaaattg cgtggcgaat acgttgtctt
     8041 tgccgacgat gcaggcaaac ggggacgggt agtcgctttc gcccaatatg gtttcgggat
     8101 tttcggggtc gaaatagtag ctgtggacga agtaaaaacg cgtgttttgg ggaatatctt
     8161 taaacagcgg gtggttttgg gtttggcgca cggtgttcca gcccatatgc gggactttca
     8221 gacggcatcc ctgccggtcg cggaggtcgc gctcaaagcg tctgactttg ccgccgaacc
     8281 agcccaagcc gtcggtgttt ccttcttcgc tgtggtcgaa taaaagttgc gcgccgacgc
     8341 agattccgaa aaagggtttg ttttttaagg catctttgac tgcctcgtcc aaaccgtctc
     8401 gttttaatgc cgccatacag tcgggcatcg cgccctgacc gggaaaaatg actttgtcgg
     8461 cgcgggacac gcgttcgggg tcgccgctta aaaagatttc ggtatttttt ccggcaagct
     8521 gccccgccgt ccggacggat ttcaatacgg aatgcaggtt gcccataccg taatcgataa
     8581 tggcggtttg catggcttcc tcctcttttt ttgcaatatg gctgcgattt taacaaacaa
     8641 atgtgccgtg ctgataaaaa tgccgtctga aaacgggagt ctgtcttcag acggcatagg
     8701 gtttaaaccc ggaaagccgt ttgtcagcct tccatttgtt ttgcctgaac ggcagtcagg
     8761 gcgatggtaa acacgatatc ttctaccagt gcgccgcggg agaggtcgtt gaccggttta
     8821 cgcaggcctt gcagcagcgg gccgacgctt aagacgttgg cgttgcgttg gacggcttta
     8881 taggtgcagt tgccggtgtt caggtcgggg aagaccaaaa cggttgcctg tcctgccacc
     8941 gggctgcccg gagctttgga tttgcccaca cccggcacgg ttgccgcatc atattgcagc
     9001 gggccgtcga tggcgaggtc ggggcgtttt tcccgggcaa gtttggttgc ttcgatgacg
     9061 gtatcgacat cggggccgct gccggagttg acggtggagt aggaaatcat cgccactttc
     9121 gggtcgatgc cgaaggcttt tgcggaatcg gcagactgga tggcgatgtc ggcaagctgt
     9181 tgcgcggtcg ggttcggatt aaccgcgcag tcgccgaaga cgaggacttg gttgggcagc
     9241 agcataaaga atacgctgga cacgaggctt gcgcccggtg cggttttaat cagttgcaaa
     9301 gcggggcgga tggtgttggc ggtggtgtga accgcaccgg ataccaaacc gtccacatca
     9361 ttttgcgcca tcatcatcgt accgagtacc acggtgtctt gcagttgctt gcgcgcgtct
     9421 tcgggtgtca ggcctttgga tttgcgcagt tcgcacatcg gctcgacgta ttgttcgacc
     9481 aatgaggcgg gatcgatgat ttccaaagag tcgggcaggc tgatgccgcg ttctttggca
     9541 acggcttcga cttcttcgcg tttggcaagc aggacgcagc gggcaatgcc tttttcgtgg
     9601 cagatggcgg cggcttggac ggtgcggggt tctgcgccct caggcaggac gatgcgtttg
     9661 tcggcttggc gggcgaagtc gatcaggttg tagcggaatt gcgccggcga caggcgtttt
     9721 gcttcgcggc ctgccaatac ggatacgtct ttcagcgcgt cgctcgaacc gaagaaggtc
     9781 aggccggttt tttcggctgc cgcttcggca acggaggctg ccgcgccgtc cacgacaaaa
     9841 ccttccaata cgcccggcgc ggcggcgaag aactgtttgg caaggttcag ccgatttgcc
     9901 agttcgtcgg catcggcgtt gtcggaacgg acggcgaaga cggctgccgc gtcaagggac
     9961 aatgccagtt cgacgttttt gcctgcgagg tagattttgt cggcatcggg cgcgatgcct
    10021 tcgatgacga ggttggcggc atcgagtgcg gcaactttgc cgaccagtgc gtcgaaccag
    10081 tcgtcgcttt tgccttgcgc gagcagggtt tcggcggttg cgtcaacggc ttggaaaatt
    10141 tgtgcgtcca gtgcttttgc aaaggcttgt gcggcggcgg aggcgtccag tccggcagat
    10201 acgggtacga tgagtacttt tgccatgata tatcctttcg tatgctgcgg tgtgcggcat
    10261 atgtggttgg aaggggcggc atataggcag aaacggctgc ctgcgtgccg tgcgtgccgt
    10321 gtttggcttg aggcgcgcag gttgaatata gcaaacaaat tctgtttcca acaagataaa
    10381 tatccgcagg cttgtggatg ctgccgcctt tcagagggta tttccgggga agaacagggc
    10441 gggaccgtcc aaatggagga cggcggaaat gccgtctgac agggtggggg cggaagggag
    10501 gttgagcgtg aggacggttt gtccgacccg gagactgatt tcggtatgcc gcgctttggg
    10561 cgtggttttg agaaccacgg cgtgaatgga ggcggtgggt gcggaatggg ggtgaaggct
    10621 gaactgttcc ggacggatga gcagtgtgcc gtgtgtgcct gcgggtgcgt cgctttgaac
    10681 aggcaggcgg cccaatccgc aatcggcggt gccgtcggcg ttgagcgcgg cggggaacac
    10741 gatgccttcg ccgataaaca gggcggcatc aaggtcggca ggttgtcggt acaattcgtg
    10801 agggcttgca gtttggagga tgcgcccctg tttcatcacg gcaatccggt cggcgtattg
    10861 cagggcttct tcgcggtcgt ggctgacgaa aacggcagat ttgccgttgg cgcgcagggc
    10921 ggcaatcatg tcttcgcgaa tctggcggcg caactgttcg tccagcgcgc tgaagggttc
    10981 gtccaacaaa atcagttcgg gatcgggtgc gagggcgcgg gcgagggcga cgcgctgttg
    11041 ctgtccgccc gaaagttcgt gcggatagcg tccggcaagt tcggaaatgc cggtcaattc
    11101 caacatagct tcgatgcgct gccgctcttg cgccgtcttg cctttgccgt tgcccagccc
    11161 gtaggcggtg ttgcggtaaa cggtcaggtg ggggaacagc acaccttcct gtacgacata
    11221 acccaaacgg cgttcgcgga cggggaggtt ggtatttttc gagaagatgg ttctgccgga
    11281 aagcgaaatt tcgccaaaat cgggttgttc aaaaccggca aggcagcgta aaagggtggt
    11341 tttgccgcag ccggacgcgc cgacgataaa gaggatttcg cccgggtcga ggctgagcga
    11401 aatgtcgttt aaaaccgggg tgttttgaaa acttttggac aggtgtccga tgtgcagggc
    11461 ggcggtcatg gcggtacttc ctcaagctgt tatttgaagg cgtatttctt cagcaggaat
    11521 acggggatgc cggaaaataa taccagcatc agcgcgtaag gggtggcggc ggcgtattgt
    11581 gcgtccgatg tgtattccca aacggcggtg gagagtgtgt ggacatcgtc ggtggtcagc
    11641 agcagggtgg cggtcagctc tttcatcagt ttgaggaaga cgagtgcgaa tgcggcggta
    11701 atgccgggca ggatggacgg cagtaccaac gtcctgaaaa taaagaagtg tccgcgcccc
    11761 aatgttgcgc cgacctgttc catccctttt gggagttgtt ccaaggaagt cctcagggtg
    11821 gtttgcgcca tcggcaggta aagcatgaaa taggcaagga tgacgacgat aaaggtttgg
    11881 taaacggcag gggtgtagtt gatgctgaaa taaaccaagg atagggcgat aaccaaaccg
    11941 gggacggcgt gcagtaaaaa cggcagcctg tctatccaaa cggttaaaaa attgcgatag
    12001 cgaaccgatg cccaaacaag gggcaaggca cataatatag tcaaaatcgc acctaaagcc
    12061 gatacgctta aggaacggat aaaggcatca aatacggata cgagcgcgaa tgtgccggaa
    12121 gtgccgacca tcatccaatg tatcaatacg ccaaagggga taataatgcc caaagtcaac
    12181 aagctgctta aaaaaacaat cgcgccgatc tgaccgggca gtttgagggt tttgacggga
    12241 taaggacggg caacgccttt gccgctgtgg taaatcttgg ctttgccgcg aaatatgctt
    12301 tctccaaata cgacgatgcc gcacaccgcc attaaaacag cggaaagcag ggcggcggta
    12361 ttgttgttgt aggacatttc gtattcttgg aaaatggcgg tggtaaaagt ggggtagttc
    12421 aaaatagata ccgcgccaaa ttcgaccagc atatgcaggg caatcagtaa cacgctgctg
    12481 ccgatggcgg gtttgagctg ggggaggatg gcggaaaaaa aggtttgcag gcggcttttg
    12541 cccaaggaca ggctgacttc ttcgtaagac aggctgatgc gtttgagtgc cgcctcgacg
    12601 ggcaggtagg cgagcgggaa cgaggacagg ctcataatca tcactgtccc ccaaaagcct
    12661 tcgacacgga aggtcaggct gatccaggtg aaacagctga caaatgcggg gatgcacaaa
    12721 ggcagggtga ttgccgtctg aaaaaaggtt ttgccgaaga agcggtaacg ttggaacaaa
    12781 agggcgcagg caatgcccaa aacaatggaa atcagggtaa cgcccgccat catcgtcaag
    12841 gtgttggaga gcaaatccca catacgcggg cggaacaaca gttcgacggc gcggttgatg
    12901 ccgacctgcc acgaacgcat agcgacatat aaaaaaggca gggtaagcgg tagggcaatc
    12961 agtaggatga ggccggtaag ccaaatgggt atttttttag gagacatagt gttttttatc
    13021 ggcaaaacgg gcggacagta taaatgtcca cccgtttgac aatccgaaaa cggcttattt
    13081 cataccggct tgctcaagca gccgggtggc gtgttctttt tcggaaacag tggtggcgga
    13141 gacttggggt gcttccaact tggcgatggg ttccaaattg aaagtggata ccacgtgcgg
    13201 attcaaagga tattcggcac ggacggcggt cagggcgcgc tgtccttcct tgctggcgag
    13261 gaaggcgacg aattttttcg cctcatcctt gttttgggag gattttaaca cggctgcgcc
    13321 ggaataggta acgagtgcgc cgggatctct gtggcggacg aaattcaggc gggtgtggac
    13381 attttgtacg cctttttcac gcgcaaaagc gtgccagtag tagttgttga tgagggcggc
    13441 atcgatttcg ccgttttcaa ccgcttgaag ggcgacggag tttttagcgt aaggcttgcc
    13501 gtattctttc agacctttga gccatttcaa tgcggccgct tcgcctttca gtttgacgat
    13561 ggcgacaacc tgttccaaga acgcgccgga agtgggggcg taaccgatgc ggtttttcca
    13621 tttcggcgtg gcgtaattca ggacggattt ttccaaatct ttttcagaca gtttgcgggt
    13681 gtcgtaaacg acgacgcgcg aacgtccgct cagtgccacc cagtcttttt tggcggcaac
    13741 cggcacgccc ttgccgcgtg tttcgttgat ggtggaggcg ggcaggggct ctaggaggtt
    13801 ggctgcggaa agggtggcga gtgccgggat ttgttcggaa tagaatacgt cggcggggct
    13861 tcggctgcct tcttctttga tttgaccggc aagctggtcg cctttggcgc tgttgagttt
    13921 gactttgatg ccggtagccc gggtaaaggc atctgcaacg gcttgtgccg cttctttgtg
    13981 ttggccgttg tacacggtaa tgtctgccag cgcgggggtt gcggcggtca gggctgcggc
    14041 aagcagtgcg tatcggatag atgttttcat atcgattttc tcctaatgaa tgagagtgta
    14101 caccttgtta agacataacg gcgtgtagtg tattccttct tctttataaa tgcaaataat
    14161 tattttttaa atttgttatt atccgatccg gttattgttt gttctgactt gtattttttc
    14221 cgtgcatcgc gcccgtaagg cggaagcggc gggcaatgcg tggcggaatg tgggtaaagg
    14281 cggcattttg atttgtcgga atgcttgaga acccctctct ttaaaacagc gtctgaacag
    14341 ggttgccgga atagtattgc catcccggca gatacagttt gtcggggtct tgccaatatt
    14401 gttcatccag actgttcggc agtgaggcgg ttttgttgtc gagatgtttt gttatatccc
    14461 acatttcttt caggttttta ccttccgatt ggaggtggcg gatttctttt ctcaatgcgt
    14521 ctgccgcccg atggatcagc attgcgctgt gtaccgcgcc gatttttttc agaacggaac
    14581 aagtcttttc ggcggcgggg aaaccccagt tgcagacaaa ttgcagtatg ccgccgttgc
    14641 cgatgtcggc ttccgcccgc caaatcaaaa ccagctcctg ttcacgtccg tccatgcttt
    14701 cgagtttgcc gtcatgccgt tcaaaaagtt tgtccaccgc ctgccacatc atctgctcga
    14761 agcggtcggc ttgggtatcc gtatcggtca tcgtgttctt gccttttaaa aatgccgtct
    14821 gaacatttct tcagacggca tttgggggtt aagccaacat ttcccgccag cgtttgactt
    14881 ggaaacggac ttgttcaggc gcggtgccgc ccaagtggtt gcgcgcgttt aagctgcctt
    14941 cgggtgtcag cacgctgtaa acgtcgtctg aaatcagctt gctgaaacct tgtaagactt
    15001 caagcgacaa ttcgctcaaa tcaacgccag cttcatcggc atggcgcacg gcttgggcaa
    15061 cgacttcgtg ggcatcgcgg aagggcatac cttttttgac cagataatcc gccaagtcgg
    15121 tggcggtggc aaagccctgc attacggcgg cgcgcatatt gtcgggtttg acggttacgc
    15181 cgcgcatcat atcggcgtaa atccgcagtg tgtcgatgag tgtgtcggcg gtgtcgaaca
    15241 agggttcttt gtcttcctga ttgtctttgt tgtacgccaa gggttgggat ttcatcaggg
    15301 taatcaggcc gataaggtgc ccgataacgc gtccggattt gccgcgcacg agttcgggca
    15361 cgtcgggatt tttcttctgc ggcatgatgg acgaacctgt gcagaaacgg tcagcgatgt
    15421 cgataaagcc gaaacgcgga ctcatccaca aaatcaattc ttcagacagg cggctcaagt
    15481 gaaccataac cagcgaggcg gcggcggtga actcgatggc aaaatcgcgg tcggatacgg
    15541 catcgagcga gttttggcag atttgttcaa agcccaacag ttcggcggtg atttcgcgcc
    15601 gaatcggata agtcgtaccg gcaagggcgg ctgcgccgag cggcatacgg ttgacgcggc
    15661 ggcggcagtc cgccatccgt tcgttatcgc ggccgagcat ttcgacgtag gcgagcatat
    15721 ggtgtccgaa gctgacgggc tgggcgactt gcaggtgggt aaagccaggc ataacggttt
    15781 cggcgttttg ttccgccaaa tccagcaatg ccgtctgaag gttttgaatc aggttttgaa
    15841 taacggtaat ctgatcgcgc agccacaggc ggatgtcggt ggcgacttgg tcgttgcggc
    15901 tgcggccggt gtgcaggcgt ttgcctgcat cgccgatttt gtcggtcagg cggcgttcga
    15961 tgttcatgtg gacatcttcc aaatcggacg accattcgat tttgccgctg cggatttctt
    16021 cgaggatttc cgccatgccc cgacggatgt ccgccaaatc gccttcgtcc aacacgccgg
    16081 tttctttcag catttgcgcg tgggcaagcg agccttggat gtcccattcg gcaaggcgtc
    16141 ggtcgaaacc gatggaggcg gtgtattgtt tgacgagttc ggaaacgggt tcgttgaaac
    16201 gtccggacca ggttttgtcg tgcataagga ttccttgatg gggttattcg gtgcggtatt
    16261 tttccaaaag ccggcggaag ggttcgccgg tttcgggatg tttcagaccg taggcgacgg
    16321 tggcttcgag gtagcccagt ttgctgccgc agtcgtagcg cgtaccttca aaggggtgcg
    16381 ccaggacaaa ttcgtgatcg agcagcttgg cgatgccgtc tgtaagctgg atttcgttgc
    16441 ccgcgccgcg cggaagattg gttaagaggt cgaaaatgcg cggcgtgaga atgtagcgtc
    16501 cgacgacggc aaggttggag ggcgcgtctt cgggcttggg tttttcgaca atgccggtaa
    16561 tgcgttggaa ctgtttgagc tgttcggttt cgacgatgcc gtatgagccg gtttgcgacg
    16621 gttcgacggt ttctacgccc aaaatgctgt tgccgctgcg ttcgtacact tcaatcattt
    16681 gtttgagcgc gccttttggg gcatcgatta aatcgtcagc caaaatcacg gcaaagggtt
    16741 cgtcgccgat ggcggcgcgg gcgcacaaga cggcgtgtcc caagcccagt gcttccgcct
    16801 gacggatgta gaggcaggta atgttcggcg gcaggatgtt gcggacgtgt tccaacaatt
    16861 tgtctttatg gcgcatttcc aactcggttt cgagttcgta tgccttgtcg aaatggtctt
    16921 cgatgctgcg tttgttgcgt ccggtaacaa acaccatttc cgtgcagccg gcttccacgg
    16981 cttcttctac ggcgtattgg atcagcggct tgtcgacgat gggcagcatt tctttcgggc
    17041 ttgccttggt ggcgggcagg aagcgtgttc ccatccctgc gacggggaaa acggctttcc
    17101 gtatgggttt cattcttttt cctttgtatt gttttgatgt ttaaagggcg agtttgcgta
    17161 agagttcggc aagtgcctgc gcgcggtggc tttcgcggtt tttgacctcc gaatccaatt
    17221 cggcggcggt tttgccgtgt ccgggcagat aaaaatacgg gtcgtaaccg aaaccgtttt
    17281 caccggcggg aacgccgttc cattgtccgt gccatacgcc ttcggcaata atcggacgcg
    17341 ggtcgtcttt atggcggaca aaaaccaata cgcagacgta gcaacagctt ttgtcggctt
    17401 tgccggcaag ttcggcggca agtttcaggt tgttggcggt atcggatttg ggattgctgc
    17461 cggcgtaacg tgcggaatgt atgcccggag caccgtttaa ggcggcggca cagatgccgc
    17521 tgtcgtcggc gagtgcgggc agcccgctgt gtccggcggc gtgccgggct tttgccagcg
    17581 cattttcaac aaaggtggaa tagggttcgg ggcattcggg tatgctgaac tcggattgcg
    17641 gcaatacggt aatgccgtat ggttcgaata ggttgccgaa ttctttgagt ttgcctgcat
    17701 tgccgcttgc caaaacgatt ttttccggtt tttcagacat agcggttttc ctttgcggcg
    17761 gattgggcgg cgcgtaggga tttgtgccgc aggtagaggg cgaggctgcc gatttgtccg
    17821 aacagtgcgc cgaatgcgaa aagaaaggag gcgacggcga aggctttgct gccgacggtc
    17881 agcagataag cacccaagag gacgagcagc ataaatgcca gtgtgaagag ggcaacagac
    17941 aacagataga tttttcggcg gttcatggcg ttcggtcgga aacggtatgt tcggattata
    18001 gccgattggg acggtattcc ctagagcttg gaaaaatgcc gtctgaaacg gcgttcagac
    18061 ggcatggcgg gcgttatgcc tgtttgttcc aacgttcgat ggattctttg atgacttttt
    18121 tcgcttcttc cgcatcgccc cacgattcga ctttggtcgt acctgctttt ttcaggtctt
    18181 tgtaatggtt gaagtggaac tcgatttgtt tgatgagctg ttgcggcaaa tcggacaaag
    18241 ttttgtaggc gttgccgttg ttgcggtcgt cggcaggaac gcagacgatt ttgtcgtcta
    18301 cttcgccgtc gtcaacgaat ttcatcacgc cgataacgcg cgcttccaag aatacgccgg
    18361 ttgccaaagg ttgttcggta acgagcagca cgtccaattc gtcgccgtct tcgtccaaag
    18421 tttggggaat gaagccgtag ttggtcggtt tggcgaagat ggcgggttcg acgcggtcga
    18481 gttggaatgc ggccagtttg cggttccatt cgattttgtg gttgctgccg gcggggattt
    18541 cgttgacaac gttgatgatg ccgccgtcca cgtcgccggg ggtcaggatt tggttgaagt
    18601 ctgccatttg gtttcctttg tttgggaaag tgtgaagttt gaaagtatag cacaaacgtc
    18661 cggctgaaaa tgcgcccgat gcctctgaaa gggtgtacgg gcgcgtgtta ccgtttgccc
    18721 aaaaacctgc ccagttccaa aatcgcgcgc ctgttggacg gggaaaatac tttttccgct
    18781 gcttcttcca tatcgaacca accgtaggag acgtgttctt cgggttgcag ggcgatgggc
    18841 gtatcacgcg ggatttcggc agagaagagg tgttcgcggt tttcaaacac gccttttgga
    18901 tagcggtgcc gccagtggtg gtagatttcg taaaccgtgc tgtcgtgcca gtcttgaagc
    18961 tgcccgtccg ccagcaggat gccggtttct tcccaaactt cgcgccttgc cgtttgggcg
    19021 acggtttcgc ccggttcgag gctgccggtt accgactgcc aaaatccttc cggatgcgtg
    19081 cgttcgatga gcaggatgcc gccgtccccg ctataaagga cgaccagtgc ggaaacgggg
    19141 tatttgagcg gttttgccat cggcatcttt cggcgggctg cggtaatgaa ggggcggatt
    19201 atagcaaacg ccgcacgtta tggcgtttat ccttttccgt atcctttttt ctgcacggat
    19261 gggacgcgcc ggtgtttgcc ggtaaatttt ccgttgtgtc aaaaagataa gggcggttgt
    19321 gattttaatg cttgccaaag cgtcgggcgg aaactataat ccgaaactta tcgagtcgga
    19381 gtgtggcgca gtctggtagc gcacttgcat ggggtgcaag gggtcgaagg ttcgaatcct
    19441 ttcactccga ccaaaaattc cgaaagccgc tttcaaaagc ggcttttttg ccgtccgtat
    19501 gattatgatg tagagtacgc ggcgacagac attcaaatgc cgtctgaaaa ccgttcagat
    19561 ggcatctctt tatcttagtt tcattccgta ccatcttaag gaacatcaaa ttgggcattt
    19621 cccgcaaaat atcccttatt ctgtccatac tggcagtgtg cctgccgatg catgcacacg
    19681 cctcagattt ggcaaacgat tcttttatcc ggcaggttct cgaccgtcag catttcgaac
    19741 ccgacgggaa ataccaccta ttcggcagca ggggggaact tgccgagcgc agcggccata
    19801 tcggattggg aaaaatacaa agccatcagt tgggcaacct gatgattcaa caggcggcca
    19861 ttaaaggaaa tatcggctac attgtccgct tttccgatca cgggcacgaa gtccattccc
    19921 ccttcgacaa ccatgcctca cattccgatt ctgatgaagc cggtagtccc gttgacggat
    19981 ttagccttta ccgcatccat tgggacggat acgaacacca tcccgccgac ggctatgacg
    20041 ggccacaggg cggcggctat cccgctccca aaggcgcgag ggatatatac agctacgaca
    20101 taaaaggcgt tgcccaaaat atccgcctca acctgaccga caaccgcagc accggacaac
    20161 ggcttgccga ccgtttccac aatgccggta gtatgctgac gcaaggagta ggcgacggat
    20221 tcaaacgcgc cacccgatac agccccgagc tggacagatc gggcaatgcc gccgaagcct
    20281 tcaacggcac tgcagatatc gttaaaaaca tcatcggcgc ggcaggagaa attgtcggcg
    20341 caggcgatgc cgtgcagggc ataagcgaag gctcaaacat tgctgtcatg cacggcttgg
    20401 gtctgctttc caccgaaaac aagatggcgc gcatcaacga tttggcagat atggcgcaac
    20461 tcaaagacta tgccgcagca gccatccgcg attgggcagt ccaaaacccc aatgccgcac
    20521 aaggcataga agccgtcagc aatatcttta tggcagccat ccccatcaaa gggattggag
    20581 ctgttcgggg aaaatacggc ttgggcggca tcacggcaca tcctatcaag cggtcgcaga
    20641 tgggcgcgat cgcattgccg aaagggaaat ccgccgtcag cgacaatttt gccgatgcgg
    20701 catacgccaa atacccgtcc ccttaccatt cccgaaatat ccgttcaaac ttggagcagc
    20761 gttacggcaa agaaaacatc acctcctcaa ccgtgccgcc gtcaaacggc aaaaatgtca
    20821 aactggcaga ccaacgccac ccgaagacag gcgtaccgtt tgacggtaaa gggtttccga
    20881 attttgagaa gcacgtgaaa tatgatacga agctcgatat tcaagaatta tcggggggcg
    20941 gtatacctaa ggctaagcct gtgtttgatg cgaaaccgag atgggaggtt gataggaagc
    21001 ttaataaatt gacaactcgt gagcaggtgg agaaaaatgt tcaggaaata aggaacggta
    21061 ataaaaacag taactttagc caacatgctc aactagagag ggaaattaat aaactaaaat
    21121 ctgccgatga aattaatttt gcagatggaa tgggaaaatt taccgatagc atgaatgaca
    21181 aggcttttag taggcttgtg aaatcagtta aagagaatgg cttcacaaat ccagttgtgg
    21241 agtacgttga aataaatgga aaagcatata tcgtaagagg aaataatagg gtttttgctg
    21301 cagaatacct tggcaggata catgaattaa aatttaaaaa agttgacttt cctgttccta
    21361 atactagttg gaaaaatcct actgatgtct tgaatgaatc aggtaatgtt aagagacctc
    21421 gttataggag taaataaaaa tggcaatttg gaattatcgg tactacctta tcccatcagc
    21481 tgctatcaga aataaattta atgcaaataa agatatcata cttgatgagt atcgatctaa
    21541 tggttttcag aattttaatg agaataaaag ttttgaaaat tactttatcg ataatgatgt
    21601 tatattatta tcaataataa atgaagcaaa aaaacagctt aaattgaaag aatcttggga
    21661 taaagacgca atcatgtttt gtgataattt tggtaatagt cttaccgttt ggccagatga
    21721 tatagagtgc gaacttgatt taagatttga ttatactaaa tttattcaga aaaccattga
    21781 ttgggcaata aaatataatt gtctacttgt aatagaaaaa acaggaaatg tagtttcccc
    21841 taatataaat aatctgatgt atgaaataaa agcatatttg gaaagcaagc cgtggcccat
    21901 atgaaaccta aactcaacaa gtaggatgtg tgcggaacgc acgtatgcgg ttctcaaggt
    21961 ttgagctaag aggccgtctg aaaacagaaa aactgtttca gacgaccttt cttttaacca
    22021 gttgccacag caaccggaca aaagcagcct acctccacat ccatataggc aatacagggg
    22081 agatattttg taaattctac gaatatttta cctgctaaac agggtaggat atggtatgaa
    22141 gcgaacattg gcttaataaa cactatgtca agatcgaatc aggctggtac tagattgttg
    22201 tattccaatt atggattgct atatataaca actgatcatt atatctctgc aacacggttt
    22261 gtagcttgga aataggagta taacttatgc aattagagat tatcggtagt aaaatttata
    22321 cggaacaaga ttttcataat caaatttcaa aaatattttc tatacaagat tattatggga
    22381 acaatcttga tgctttatgg gatttattaa gcacaaatgt agaacgaccg attactttgg
    22441 tatggaaaga tgctatgttc tcaaaaaatc aattagaaaa tatatttatt gaaatcgtaa
    22501 atgttctaga aagagttaag aaacaagatg aggattatgg attcgaagaa aaatttaatt
    22561 atattttaga gtaagtaaac cctaattaca ttacgtacac aggcttaaaa ctccccagag
    22621 ccaattaagc aagccgtaac ccatataaaa cttaaactca acaagtagca tgtgtgcgga
    22681 acgtacgcat gcggttctta aagtttgagc taagaggccg tctaaaaaca gaaaaaccgt
    22741 ttcagacggt ctttgtttaa cgccaccgat ccagcgggtt acaaagcgca gtcaatgccg
    22801 ctgcgcctta tgcctccgaa gcaataggca gaacatttgg acacggtgaa aacaaaaacg
    22861 aaaccgccca agccgtcgga catttccttt taggagcagc tattgcccgc gtcaacggtg
    22921 gtaattttgc tgccggcggc tcggcagcag ttgcagctga aaaggcggcg gaacatcttg
    22981 cccaacagta taacgacggt aaaaccgcaa tcgatccgca aacaggcgag ttcaatgcca
    23041 acctgctgcc ggaacatatc aaagaggaaa tcaaatcaaa gagcggggtg attgcatcgc
    23101 tgacgggcgc ggccgtgggc ggcacgccgg tagatgcgca aaccggaggt gcggtcggac
    23161 agaatgcggt ggaaaacaac ctctatctga catcggaagc cttaaagaag gacaagcaga
    23221 cagctcgtaa aatttattcc gtcataaaag agcaagtcaa gcatgaatgc agttccacag
    23281 gaagaattac cgaatgtcgt caaaatatag gacgcattat cgaatttacc caagacaaac
    23341 gctttgacag taggtttaag gacttaaaaa aagaatcctt atattaccta aataaacatc
    23401 ctgatttagt agcctcttat ttgaaggctg aatacgaaaa gctggatagg gaagacaaaa
    23461 gtatcctgca ccgctacatc tcacccgggg ctgaaatcgt ttcgggcagt ttgggggttg
    23521 ttctttcagg agtagccgga ggcggatctt gtgccgagac tttcggctta ggctgtgccg
    23581 ccgctttggt tggtgtaacg tcttcctacg atcatgtcat tactgggacg aaaaacttcg
    23641 gaaaaaaagc cagcgagcaa cgaccgacga ttgcggttca ggccttgaag cagttggggc
    23701 tgtcggagca ggctgcggaa tatgttcagt tctctataga tttgttcagt gtgggtaaat
    23761 cggggggcgg tatacctaag gctaagcctg tgtttgatgc gaaaccgaga tgggaggttg
    23821 ataggaagct taataaattg acaactcgtg agcaggtgga gaaaaatgtt caggaaacga
    23881 gaagaaggag tcagagtagt cagtttaaag cccatgcgca acgagaatgg gaaaataaaa
    23941 cagggttaga ttttaatcat tttataggtg gtgatatcaa taagaaaggc acagtaacag
    24001 gagggcatag tctaacccgt ggtgatgtac gggtgataca acaaacctcg gcacctgata
    24061 aacatggggt ttatcaagcg acagtggaaa ttaaaaagcc tgatggaagt tgggaaatga
    24121 aaaaaggaag gggcggaaaa cctatgacaa atcattctat gttcccaaaa gattgggatg
    24181 aggctagaat tagggctgaa gttacttcgg cttgggagaa aagaaaggaa ttagaaggtc
    24241 aaaaatggag aggtacaagt aaatctggtg ttgaaataga aggatttacg gaacctaatc
    24301 gaacagctta tccactttat aatagtaaat gaagtattga ttaaaaaggt gtttttaaat
    24361 gtataaaata aaatgttttt atgatcagtt tagttctggt gaattattta actactgcca
    24421 gtttttagat aattcttcta atcaaaagat aaatacacga ggaacggtgt atcaatatat
    24481 tatttatgtt ttaacaggag acctttatct acaaaaagac atagatgaaa atttagaatt
    24541 tatccatcaa gcagaaaata atcccaatga agtttattca ggaggtggac aaggtttttg
    24601 ctgggatatt tctgctgaaa aagtagtttt ctatcacaac gaatttgatg aagaagacgg
    24661 ctggcctgat ttaagctgtt cgctgcatac ttttaaaact gctttaattg cttggaatgc
    24721 ttttttgcaa ttgcctaaaa gtattcattc ggtggtggag actgtgattg aggaataagc
    24781 ataattagct taatgaatag aatcagcgat atagattgga ctgcaaatcc acgcttatac
    24841 gctgtgccat gattaacagg aagagagcaa cttggatggg ctagtaggga tggtaagcat
    24901 ttaaatatat caattgatgg aaagattaca cacaaatgac taatttaaaa ttagatttct
    24961 actctgaagt tattataaaa gattcttgcc ctaatgattt gttagaaaat ggtgagacta
    25021 ttaaaggaaa aaaaggagtg gtattaggta taagcgaaga agatggtata atctatggct
    25081 atactatttt actttttgat ataaaatatt gtatatatat agataagaaa tatattatac
    25141 cgactggaaa aaaattctct cgcgatgatt tttattagct aatttaagta atttaagcaa
    25201 gttttttaga tactgaaact ggtaattatg tttatgtaag cataaggagt tcagattgaa
    25261 ggattctaca gggcaaatga ggctggcaac caaggatttg gcggaagcca ttaaacgagg
    25321 agaagtacgt agttctgctt ttacaacaaa gcaactaaag gcaatcgaaa aaggagaatc
    25381 taaaataccg gattacactt ggcatcatca tcaagacact ggaaggatgc aattgattaa
    25441 tgaagacttg catcatgata ccggccatat cggttggaga gcgatgagta aaggaaagta
    25501 actatgtgga aaatcataaa agaggatagt gatgatttag gatttgcaat taaatgctta
    25561 ttctctcaat ctattgattt aaatgaattc aagttatgga ttgaacaagt aatacgcgat
    25621 atgcccattg aggacatccc tttttatatt tttgacttgg cggatttcga tgtaggaatt
    25681 ggcgatattg gcaatattgt aggttttgct ccgagttcca gcctgccgaa atcgaaaaaa
    25741 aatgccttga ccggcattgc cttcttaagg gggatagatg tctatgaccc tcccgtttca
    25801 aaagaaaaag cattaaaagc cttagagaaa tatcctgaaa tttatcagaa atttcagcat
    25861 ttctttccgt ttgtagagct gcctccgctt taaacagtca aaatgccgtc tgaaacaata
    25921 ttcggctttc agacggcatt tttgatacaa agcaggtaac caaaagagcg tttgacggca
    25981 aaggaagata attatgtgga aaattacgaa agagcaaggt caagatttaa gttttgcaac
    26041 atattgctta ttatcggcag caattaattt gcaagaattt aaattatggt tggagaaggt
    26101 tgtcttagat atgcctattg ataatataca tttttatatt tttgatttaa ttgatcttaa
    26161 agaaggagta ggagatattt ataatatttt ggattttgtt ccgaattcag acttatctaa
    26221 agaccaagat gacgcattaa caggcattgc cttcttaagg ggtatagatg tctatgaccc
    26281 tcccgtttca aaagaaaaag cattaaaagc cttaaagaaa catcctgaga cctttgcaaa
    26341 attcctttcc ctcccgacag ccgaaaccca aacacaggtt ttcggctgtt ttcgctacca
    26401 atcactccct aattctaccc aaataccccc ttaatcctcc ccggataccc gataatcagg
    26461 catcagggct gccttttagg cggcagcggg cgcacttagc ctgttggccg ctttcaacag
    26521 gttcaaacac atcgctttca gatggctttg cgca
//
LOCUS       DAXHKX010000008        25958 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHKX010000008 DAXHKX010000000
VERSION     DAXHKX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25958
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.008"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            661..2109
                     /gene="gnd"
                     /locus_tag="ACFMWZ_000325"
     CDS             661..2109
                     /gene="gnd"
                     /locus_tag="ACFMWZ_000325"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC5934347.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFIEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2172..3443
                     /gene="waaA"
                     /locus_tag="ACFMWZ_000326"
     CDS             2172..3443
                     /gene="waaA"
                     /locus_tag="ACFMWZ_000326"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC5934348.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3484..3933
                     /locus_tag="ACFMWZ_000327"
     CDS             3484..3933
                     /locus_tag="ACFMWZ_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC5934349.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            3966..4940
                     /locus_tag="ACFMWZ_000328"
     CDS             3966..4940
                     /locus_tag="ACFMWZ_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC5934350.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFVFLKYGLA
                     FVLGFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            5009..5084
                     /locus_tag="ACFMWZ_000329"
     tRNA            5009..5084
                     /locus_tag="ACFMWZ_000329"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5042..5044,aa:Lys,seq:ttt)
     gene            5303..6556
                     /gene="murA"
                     /locus_tag="ACFMWZ_000330"
     CDS             5303..6556
                     /gene="murA"
                     /locus_tag="ACFMWZ_000330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC5934351.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGA
                     GELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6637..7815
                     /locus_tag="ACFMWZ_000331"
     CDS             6637..7815
                     /locus_tag="ACFMWZ_000331"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC5934352.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACVGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADEEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7882..8130)
                     /locus_tag="ACFMWZ_000332"
     CDS             complement(7882..8130)
                     /locus_tag="ACFMWZ_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC5934353.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8230..9147)
                     /gene="ftsX"
                     /locus_tag="ACFMWZ_000333"
     CDS             complement(8230..9147)
                     /gene="ftsX"
                     /locus_tag="ACFMWZ_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC5934354.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9144..9794)
                     /gene="ftsE"
                     /locus_tag="ACFMWZ_000334"
     CDS             complement(9144..9794)
                     /gene="ftsE"
                     /locus_tag="ACFMWZ_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC5934355.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9988..10470)
                     /locus_tag="ACFMWZ_000335"
     CDS             complement(9988..10470)
                     /locus_tag="ACFMWZ_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC5934356.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTDAVKLAHSKCR
                     "
     gene            10594..10947
                     /gene="arsC"
                     /locus_tag="ACFMWZ_000336"
     CDS             10594..10947
                     /gene="arsC"
                     /locus_tag="ACFMWZ_000336"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC5934357.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNNALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10944..11600
                     /locus_tag="ACFMWZ_000337"
     CDS             10944..11600
                     /locus_tag="ACFMWZ_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC5934358.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11716..13110
                     /gene="gltX"
                     /locus_tag="ACFMWZ_000338"
     CDS             11716..13110
                     /gene="gltX"
                     /locus_tag="ACFMWZ_000338"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC5934359.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPADVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITTNEKLAEMVKPRLA
                     LRDIHETSKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13131..13526
                     /locus_tag="ACFMWZ_000339"
     CDS             13131..13526
                     /locus_tag="ACFMWZ_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934360.1"
                     /translation="MPSDVGIRLQTAFKRKLNMKKILYFLMFVFSTSVWAGYAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13530..14021
                     /locus_tag="ACFMWZ_000340"
     CDS             13530..14021
                     /locus_tag="ACFMWZ_000340"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC5934361.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGILLGSAAVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14144..16738)
                     /gene="mutS"
                     /locus_tag="ACFMWZ_000341"
     CDS             complement(14144..16738)
                     /gene="mutS"
                     /locus_tag="ACFMWZ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC5934362.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAVEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILATALENLDPDRLTPREALSELYRL
                     KDLCKSVS"
     gene            17182..18186
                     /gene="gap"
                     /locus_tag="ACFMWZ_000342"
     CDS             17182..18186
                     /gene="gap"
                     /locus_tag="ACFMWZ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC5934363.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQNILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18256..18375)
                     /locus_tag="ACFMWZ_000343"
                     /pseudo
     CDS             complement(18256..18375)
                     /locus_tag="ACFMWZ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18444..19124)
                     /gene="tsaA"
                     /locus_tag="ACFMWZ_000344"
     CDS             complement(18444..19124)
                     /gene="tsaA"
                     /locus_tag="ACFMWZ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC5934364.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLASAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19239..19874
                     /locus_tag="ACFMWZ_000345"
     CDS             19239..19874
                     /locus_tag="ACFMWZ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC5934365.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGNWQVIVNEAACRGIAPDTIETAWREMSSAGAVILKNAEEIKKYINNQ"
     gene            19912..20880
                     /gene="waaC"
                     /locus_tag="ACFMWZ_000346"
     CDS             19912..20880
                     /gene="waaC"
                     /locus_tag="ACFMWZ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC5934366.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFTDI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            21106..21855
                     /locus_tag="ACFMWZ_000347"
     CDS             21106..21855
                     /locus_tag="ACFMWZ_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC5934367.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIECGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            21866..22801
                     /locus_tag="ACFMWZ_000348"
     CDS             21866..22801
                     /locus_tag="ACFMWZ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC5934368.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            22864..23475
                     /locus_tag="ACFMWZ_000349"
     CDS             22864..23475
                     /locus_tag="ACFMWZ_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC5934369.1"
                     /translation="MITTYKTITSLTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(23527..23844)
                     /locus_tag="ACFMWZ_000350"
     CDS             complement(23527..23844)
                     /locus_tag="ACFMWZ_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC5934370.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24002..25273
                     /gene="purD"
                     /locus_tag="ACFMWZ_000351"
     CDS             24002..25273
                     /gene="purD"
                     /locus_tag="ACFMWZ_000351"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC5934371.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGVKAEGYEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25307..25909
                     /locus_tag="ACFMWZ_000352"
     CDS             25307..25909
                     /locus_tag="ACFMWZ_000352"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC5934372.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6198 a         5941 c         7355 g         6464 t
ORIGIN      
        1 actttcgtgg gaatgatggg atgtaggttc gtgggaatga cgcggtacag gtttccgtta
       61 cgtatcccca tccgaaactt tacttataca caagcaatcc aaagttatct gactgaaata
      121 ttgtgagatt tacccgcaaa tctcattttt tccaccactt atacacaaat tcggatgcgc
      181 actctgccga tttgcagaaa taagacattc gtgccgacac ctgaccattt ccacaagttc
      241 caaacacgct tccggcttaa atctccacag cattcccgtt cttaaaggca ccggcagcca
      301 ttcggtaaag aaaagtagga tgccatctgc acaacaagca aactatccac aaaacttatt
      361 ccccataaaa aacaatccac aggtatgcac aaaagggaat ccgccttacc gacaccctaa
      421 aaaaaggtaa tcctgatgat ttttaaaata aaaaaaagtt acccacagaa aatttctcct
      481 atcaatataa tcatcgtttt tttatttaat ttttatattt tatttataag ccttcaggga
      541 aacgcacggt aaagcgtaaa ggcatctttt caaaaaatta aaaagttaag gattgttaaa
      601 caagtgtaaa tcaggtagag ttgcggtcga taaattaaat ctataacgga gcttcctgca
      661 atgaaaggcg atattggcgt aatcggtttg gcggttatgg ggcaaaacct gattttgaat
      721 atgaacgatt gcggatttaa ggtggttgcc tacaaccgaa caatcggtaa ggtggacgaa
      781 tttttaaacg gcgcggctaa gggaacggat attgtcggcg cgtattccct gcaagacttg
      841 gtcgataagc tggcaaaacc gagaaaaatc atgatgatgg ttcgcgcagg ttcggtcgtc
      901 gacgacttta tcgaacaact gcttccgttg ttggaagaag gcgacatcat tatcgacggc
      961 ggcaatgcca attaccccga cacgacacgg cggacacatt accttgccga aaaagggatt
     1021 ttgtttgtcg gggcgggcgt atccggcggc gaagaaggcg cgcggcacgg cccgtccatt
     1081 atgccgggcg gggataagcg cgcttgggaa gccgtcaaac cgatttttca ggcgattgcc
     1141 gccaaaacgc cgcagggcga accgtgttgt gactgggtcg gcagggacgg cgcggggcat
     1201 tttgtcaaaa tggtgcataa cggcatcgaa tacggcgata tgcagttgat ttgcgaagcg
     1261 taccagttta tgaaagacgg cttggggctg tcttacgacg aaatgcaccg cgtgtttgcc
     1321 gagtggaaca aaaccgagct ggattcttat ctgattgaaa tcactgcggc aattttgggc
     1381 tataaggacg aaggcggcga accgctggtc gagaaaatcc tcgatacggc agggcaaaaa
     1441 ggcacgggca aatggacggg catcaatgct ttggatttgg gtattccgct gacgctgatt
     1501 tccgaagcgg tattcgcccg ctgcgtttcg tcgttcatag aacagcgcgt gcagaccggc
     1561 aagctgttcg cccgaaccgt tacgcccgtc gaaggcggca aacaagaatg ggtcgaggcg
     1621 ttgagacagg ccctgcttgc atctaaaatc atttcctacg cacaaggctt tatgctgatc
     1681 cgcgaagcgg gcgaaagcta cggctgggat ttggactacg gcaacactgc gctgctgtgg
     1741 cgcgaggggt gcatcattcg cagcgcattc ttaagcaata tccgcgacgc gtatgaaaac
     1801 aatcccgatt tggtgttctt gggtgcggat ccgtatttca aaaatatttt ggaaaactgc
     1861 ctgccggcgt ggcgcaaggt ggttgccaag gcggtcgaat gcggcatccc tatgccctgc
     1921 atggcatcgg cgattacgtt cttggacggc tacacgaccg aacgtctgcc tgccaacctc
     1981 ttgcaggcac agcgcgacta cttcggcgcg cacacgtacg aacgcaccga caaaccgcgc
     2041 ggcgagtttt tccataccaa ctggacgggc aagggcggag ataccgcttc gacaacctac
     2101 gatatttgat tccgttgccg aaatgccgtc cgaaccgttc ggacggcatt tgttttgcag
     2161 gaaatagaaa gatgttccaa tggctttatg atgtattgtg gctgcttgcg ccgatatgga
     2221 tacggcgtta tttggacaaa cgctccggaa gtgccccggc atatcgggcg catcggggcg
     2281 agcgtttcgg caagccgtat ccgaatcccg ttaccggcgc ggtttggata cacgccgttt
     2341 cggtcgggga aacgcgtgcc gcccaacctt tgatacgcga gttgcggcag cgttttcccg
     2401 atgcgccgct gctgatgacg cagatgaccc cgacgggacg ggaaaccgcg caagttctgt
     2461 ttcccgatgc gcaatgccgc tatcttccgt atgacaaaaa aacgtgggta cggcagtttt
     2521 tgcgcgaaca ccgcccgatg ttcggcgtat tgatggaaac ggaaatctgg cccaacctaa
     2581 tgaaggaatg ccggcgcgcg ggtgtgccgc tgtttttggc gaatgcgcgg ctgtcggaaa
     2641 aatcgttgaa cggttatctg aaagtccgcc gcctgatccg tcctgccgcc gcttcgctga
     2701 cgggttgtct ggcgcagacg gaggcggatg cggcgcggtt ggcgaaattg ggcgcggcat
     2761 ccgtgcaggt gtgcggcaat accaaatacg acctgatgcc gtcggaacag atgaaaacgc
     2821 tggcggggca gtttgaaaaa cgcatcggag gccggccggt tgccgtgtgc ggcagcacgc
     2881 gcgtttatcg gggcgaagac gaggcggaaa aactgctggc ggcgtggcaa caatatcgcg
     2941 gcgatgcgct gctggtcgtc gtgccgcgcc atcccgagca ttttcagacg gtatttgaaa
     3001 cggcaaaacg cttcgggttt aaggttcagc ggcgcagcga cggtttgccg gtcgaacctg
     3061 atacgcaggt gtggataggc gacagtatgg gcgagctgta tgcgtattac ctgtgcgccg
     3121 atgttgcttt tgtcggcggc agtctggtcg attcgggctg tcagaacatc atcgaaccgc
     3181 tttcctgcgg cgttccgacg atattcggct tttcgaccta caatttttcc gaagcctgcc
     3241 ggcacgcctt ggcatcgggt gcggcggttc aagtcgaatc ggcggatgcg tggcgtgaag
     3301 ccgttgaaaa aaccttatcg tccgaggggg gcggaatgca gatgcaggcg cgcgtggacg
     3361 gctttatcgc acaacatcgc ggagcgggcg cgagaatcgc cgaggcggtg cgggaagcgg
     3421 tatgcggaca tcgggggcga tagtgataca atccgtatcc gagttttccg gttggagtac
     3481 cgtatgagta tttatgccgt cgcgcacatc gtccacctgt attgcgccat tgcctttgtc
     3541 ggcggcgtgt tttttgaagt gctggttttg tccgtcctgc atacgggacg ggtgtcgcgc
     3601 gaggcgcggc gcgaagtgga aaaggcaatg tcttaccgcg ccgtcagggt gatgccgttt
     3661 gtggtcggac tgctgttcgc cagcggcatc gtgatggcgg caaaccgcta tctttctata
     3721 ttgggcgaac cgtttgccac ttccttcggt acgatgctga cgctgaaaat cctgttggcg
     3781 ttcagcgtgt tggcgcactt cgccatcgcc gtcgtcaaaa tggcgcgttc cacactgacc
     3841 gtcggctggt cgaaatacat acacaccgtc gtctttaccc atatgctgct gattgtcttt
     3901 ttggcaaaag cgatgtttta tatcagctgg taacacaact acatttcaaa tcaggagatt
     3961 gaaccatgac agaatatccc ggcatcgggt cgccgttgtt ttacggcgtt ttttttgcgg
     4021 cagtgctggt catgattgcc ttggatatgt tttcgctgaa gaaaaacagc agccataaag
     4081 tcggcgtcaa agaagccttg gcatggagcg gcttgtgggt tgccgtatcc tgcctgttcg
     4141 caggctggct gtatttcaaa ctcgccggca atcccggcta cggcgcggca gtcgcaaaag
     4201 aaaaagtatt ggaattcttt accggctaca ttttggaaaa atcgttggca gtcgacaata
     4261 ttttcgtgtt cctgatgata ttcggctact tcaaagtcgc accgcagttt cagcaccgcg
     4321 tgctgctgta cggcgtattg ggcgcattgg tattgcgcac tgtcatgatt ttcgtcggcg
     4381 cggcactggt tcagcagttc gagtggattc tgtatctgtt cggcgcgttc ctgctctata
     4441 ccggcataca catgatgaag cccgaaggcg atgaggaagg ggatttggca aacagcaggc
     4501 tgctgaatgc cgtcaagaaa gtcgttccgg tcggcacgga atttcacggc gagaaatttt
     4561 ttactgtcga aaacggtaaa aaaatcgcca cgccgctgtt tttggtgctg gtcatgattg
     4621 aattgagcga tgtcgttttt gccgtggaca gcatccccgc cgtctttgcc gttaccaccg
     4681 atccgtttat cgtgctgact tccaatattt tcgcaattct gggcttgcgg gcgatgtatt
     4741 tcctgctggc ggatgtggcg gaacgcttcg tattcctgaa atacggcttg gcattcgtgt
     4801 tgggttttat cggtgtgaaa atgctgataa tgcattgggt gcatatcccg atttccgttt
     4861 cgctgtcggt cgtgttcggc gcattgggtg catcggtgtt gacttcattg gtttatacga
     4921 aaaaacagtc cgataaataa aatgccgtct caacgccgac aaaatggttg acagcatcag
     4981 gcttgatgta tagaatttcg agcttgtcgg gtcgttagct cagtcggtag agcagcggac
     5041 ttttaatccg ttggtcgagc gttcgaatcg ctcacgaccc accagataaa acaaaaaagc
     5101 tgagttttca aaactcggct ttttttgttt tccgacaatc cttgttcgga aagcgcggca
     5161 tttcgtcaaa ttctttggtg cggcttccgt atttttcccg aaaatccctt ataattccgc
     5221 ctctgcaacc ttcatcgggt gatgctttat gcgttgtccc gagtattgaa atattacaag
     5281 tacccaccag gaaagaacaa acgtggacaa actgaaaatc tccgcaaacg gcccgctcaa
     5341 cggggaaata acggtctcgg gcgcgaaaaa cgcggcattg ccgctgatgt gcgcgggttt
     5401 gctgacatcg ggtacgttgc gcctgaaaaa cgtccctatg ctggcagatg tgaaaaccac
     5461 gcaaaagctg cttcagggga tgggcgcgcg cgtcctgact gacaatatca gcgaatttga
     5521 aatcaacggc ggtacggtaa acaatacctg cgccccttac gaattggtac ggacgatgcg
     5581 ggcctcgatt ttggtgctgg ggcctacttt ggcacgtttc ggcgaggcgc aagtcagcct
     5641 gccgggcggc tgcgccatcg gttcgcgccc cgtcgatcag catttgaaag gcttggaagc
     5701 gatgggtgct gagattgtta tcgaacacgg ttacgtcaaa gccaaaggca aactcaaagg
     5761 tacgcgcgtg gcgatggatg tcgttaccgt cggcggcacg gaaaacctgc tgatggcggc
     5821 gacgctggcg gaaggtacga cggttttgga aaactgcgcc attgagcctg aagtggtcga
     5881 tttggcggaa tgcctggtca aaatgggcgc gaaaatcagc ggcatcggta cgtccacgat
     5941 ggttgtggaa ggcgcgggcg agctacacgg ctgcgaacac agcgtcgtcc ccgaccggat
     6001 tgaagcgggg acgtttctgt gcgcggtggc gataaccggc ggcagggtgg ttttgcggaa
     6061 tgccgccccg aaaacgatgg aagtggtgtt ggacaaactg gttgaggcag gtgcggtgat
     6121 tgaggcgggc gatgattgga tcgccatcga tatgcggcag cgtccgaagg cggtggacat
     6181 ccgcacggtc gtccaccccg gcttccccac ggatatgcag gcgcagttta tggcattgaa
     6241 tgccgtggcg gagggaagct gccgcgtggt ggaaacgatt tttgaaaacc gctttatgca
     6301 cgtccccgag ttgaaccgga tgggggcgaa cattacaacc gagggcaata cggcatttgt
     6361 gcagggtgtg gaacggcttt ccggcgcagt cgtcaaggcg acggatttgc gtgcgtccgc
     6421 aagcctcgtt atcgccggtt tggcggcgcg cggcgaaacc gtggtcgaac agatttacca
     6481 cttggatcgc ggttatgaaa atattgaaaa aaaactcggc agcgtcggcg cgaaaatcga
     6541 gcgcgtatcc ggctgatcgg gttgcgatgc cgtctgaagc cttcagacgg catcaattgt
     6601 ttctgtatcg caaacactga ttgaaaagga ctgattatgg catttttaaa actgaccgaa
     6661 caaaacgtgc agggcaaaac cgtcctcatc cgcgccgata tgaacgtgcc gttcaaagac
     6721 ggcaaaatca gcgacgacac ccgtatccgc gcctcgctcg cgtccatcca atactgcatg
     6781 gacaacggcg catccgttat cgtgatgatc cacttgggtc gcccgactga aggcgagttc
     6841 caccccgaag acgacgttgc gcccgttgcc gcgcatttgg gcggtctgtt gggcaaagac
     6901 gtgaaagtat tgaacgactg gcgcgaaaac aaaccggctt tgaacgcggg cgatgtcgtg
     6961 atgctgcaaa acgtgcgtat caacaaaggc gagaagaaaa acgatttgga actgggtaaa
     7021 gcctacgctt ctttgtgcga cgtgttcgtc aatgacgcgt tcggcaccgc gcaccgcgcc
     7081 caagcttcga ccgaagccgt cgctcaagcc gcgcccgttg cctgcgtcgg cgtattgatg
     7141 gcgggcgaac tcgacgcttt gggcaaagcc ctgaaacagc ccgcgcgccc gatggttgcc
     7201 atcgttgccg gcagcaaagt gtccaccaag ctgaccatcc tcgaatcgct ggcggacaaa
     7261 gtcgaccaac tcatcgtcgg cggcggtatc gccaacacct tcctgttggc ggaaggtaaa
     7321 gccatcggca aatctttggc ggaacacgat ttggtggaag aatccaaaaa aattatggca
     7381 aaaatggcgg ccaaaggcgg ttctgtgccg ctgccgaccg atgtcgtcgt tgccaaagcc
     7441 tttgccgctg atgaggaagc ggtggtaaaa gacattgccg acgttgccga agacgagatg
     7501 attttggaca tcggcccgaa atctgccgcc gcgcttgccg atttgcttaa agccgccggt
     7561 acggtcgtat ggaacgggcc ggtcggcgtg tttgagtttg accagttcgc aggcggtacg
     7621 aaagcccttg ccgaagccat tgcccaaagc aaagcgttct cgattgcggg cggcggcgac
     7681 acgctggcgg cgattgccaa attcggcgtt accgaacaaa tcggctacat ctccaccgga
     7741 ggcggtgcgt tccttgagtt cttggaaggc aaagagctgc ctgccgtagc tgctttggaa
     7801 aaacgcggcg cgtaagcggt ttgatataaa aaatgccgtc tgaaacgttg tgctgtttct
     7861 gacggcattt ttgtgtggcg gttattgtgc tttggctgcc cattccgccg gagtggcggc
     7921 aaccgaaatg gaaagtgcgt gcagttcgtt actttccagt tgggctttga gtccgtcttt
     7981 aatcaggcgg tggcgcgcga ggcgtgcctt gccttcaaat tctgatgaaa cgatgacggc
     8041 gaaaaaatgg tgtccgtcgc cttctacttc gatatgttcg cattttgcca cgccttcaat
     8101 catggctttt acttgttccg aggtcagcat ggtaggttcc ttgttggaaa aatgcgccat
     8161 tatactggca aattgaaatg ccgtctgaaa acgggttcgg acggcatttt tgacggtgtt
     8221 ttattttttt tattttttgg ctttgaagcc gagcaggtgc tgggtggtgg caagccacgc
     8281 gccgaatacg cccaacgcga tgacgaagcc gaacaccagc acgatttctc ccgggtagaa
     8341 gaaacgccag ccgatgttca agccgtaggg tttgaaaatg gcatcgacca atgggcgcac
     8401 ggcggagagc agccaaccgc aaagccccaa gctgacggcg gcggaaagga tgctctgcca
     8461 catggcttga taaaggaatg ggcggcggat aaacgacgcg ggcgcgccca agagtttggt
     8521 gatttcgatt tcttcttttc ggctgaggat ttgtaggcgg atggtgttgt gcgcgacaag
     8581 gacgaacgcc atacccagcg tcagggagag aaaccataag attttacgga tgaattcgtt
     8641 gatttggtac agtgtctgta cccattccgt atccatgttg gcggattcga ccataggcag
     8701 tttggtaatg tctcggtaga ttgcctgcat ttgggcgggc gtggttgccg ggtcgggggt
     8761 aacgataaag acatccggca gggggttgcc gtcaagcatg gaaatcagat tttggtcgag
     8821 attggactgt aattcttcca aaccgtcttc tttgccgatg aagcggatgt tgtcgagccg
     8881 tttgtcgcgc gccagcaggc tgcggacggt atcgctgtcg ctttgtgcgg cggcggtttc
     8941 catatagacg gtgatttgcg gcgattcatt gagtttgccc aacacgcttt gcccgctttg
     9001 gatgcccaaa tacataaaca gcggcagggt catggcgacg gcgagcatta tgagggtaag
     9061 cagtgtgccg aaaggttggc gcagaagctg tttgagtgcg gtgcgcgcgg attcgacgtg
     9121 cagcgagagg tagtggatga tgctcatgcg agtcgtcctt tcgagaggcg caggatgcgg
     9181 tgtccgtagt ccgccatcag ggtttcgtca tgggcggcaa cgatgacggt agttcctgct
     9241 tcgtggaagg ttttgaacaa ttccatgatg tcgagcgcgt aggcgcggtc gaggttggcg
     9301 gagggttcgt cggcaatcag caggccgggc tggtgaacga cggcgcgggc gatgcacagg
     9361 cgttgttgtt caccgccgga gagggttacg ggatcgtcca attctcgtcc tttcaggccg
     9421 actttttcga tggcgatgcg ggcgcgttct tcggctttgc gcggcggata gccgataatc
     9481 cgaagcggca ggatgacgtt ttgcaggacg ttgcggtcgt agaggatttt gtggtcttgg
     9541 aacacgatgc cgatatgttg gcgcataaag ccgatttggt tgtcggtcag tgtgccgagg
     9601 tcttggttgt tgaagtggac tttgcccctg cttggtttgg tgatgcccga aatcagcttg
     9661 aggacggtgg atttgcctga accggagtgt cctgcgataa aaatcatctc gcctttgttg
     9721 atttggaagc tgacgttttt cagggcttca aaaccgccgg gataggtttt ggaaacttgt
     9781 tcgaaacgga tcataggagg tcctgtaagg aagggcggca tatcgtttcg ggcggttgcg
     9841 tccgcaggga tatgccgtct gaacaatagg taagacagtg ccgattataa agattccgcc
     9901 tgcttttaga tagtggataa gtttggcgga tatgaaggtc gggcgggaaa aatgccgtct
     9961 gaagcggctt cagacggcat ccggcgttta acggcatttt gaatgggcga gtttgacggc
    10021 gtcggtcagg cttttttcgt tcacttcccc ggtaatggtt tgtttgtatc cgcatttcgg
    10081 ggcttcgacg acggtaaagg gcagtacgcc gacattgttt ccgtaggatt tcataaagtt
    10141 tcggctgttc gccccggtgt aacgccaaat cgggtaggaa acaggggttt gcttgaggaa
    10201 gttgccgata ttgtcggatg tgtcgagcgc gatgccgacc atatcgacac tgcctttttt
    10261 ctgcgctttg taccatttgg acatggcagg catctctttt cggcacgggc cgcaccaggt
    10321 tgcccaaagg ttgacgatgc gtacgggggc tttgagtgat tgcaggcttt gcggggtgtt
    10381 gtctttccat ccggccagtt cgtctgccga ggcggtatgt gcggcaaatg tggcggcaag
    10441 ggtgattgcg gcaagattca agtatttcat cgttttgtcc tttgtatgtc gattcgggta
    10501 ttgtaaaccg tttggtacgg ttgtaggcgg gatattgagt aaaaccctgt gtaagccgat
    10561 aataagggga ttgtcgagaa gtaaggaaat gtcatgtctg aaatcaaaat tttccacaat
    10621 ccgcgttgca gcaaatcgcg tgccgccttg tccctattgg aagaacgcgg cattgctgcc
    10681 gaagtggtca aatatttgga tacgccgccc gacttgtccg aattgaagga tattttcaac
    10741 aaattgggct tggagtcggc gcgcgggatg atgcgtgtga aagatgattt gtacaaggaa
    10801 ttgggtttgg acaaccccga tttggacaat aacgcactgc tgcgtgccat cgccgatcat
    10861 cccgccctgt tggagcgtcc gattgttttg gcaaacggta aggcggctgt cggcagaccg
    10921 ctggaaaata tcgaagcggt attgtgaaca aacggctgtt ttgcagccgc aacggcttgc
    10981 gctattacct gttgggcggt ttctgtttga gtgtttttcc gcttttgctg gtgtttgcct
    11041 catccgtttg ggcggtttat cggacgggcg ggcaggtttt gccgccgtat gtccgggcgg
    11101 atgccgcgct ggtgttgggt gccgccgcat gggacaaacg cccttcccct gtttttcggg
    11161 aacgcatcaa ccacgccatc gcgctttacc aaagccgcag ggtaggcaaa atcgtgttta
    11221 cgggagggcg aaccaaaaaa ggctatatga ccgaagccga ggtcgggcgg cggtacgcgc
    11281 tcaaacaggg ggttccggcg cgcaacatcc tgtttgaaaa cacttcgcgc aacacttatg
    11341 aaaacctgaa caacatccgt ccggttttgc gtgccaacgg tattgacagc gtggtgattg
    11401 tcagcgatcc ctaccatttg gcgcgggcgg cggaaatggc ggaggatttg ggtgtccgcg
    11461 tttatatgtc tgccaccccg accacgcggt ttgatgcggg aaataagaaa aaaatcttta
    11521 tgctgcaaga gggatatgcg ttatccctct accgtttgga aaaatggggc agccgctttt
    11581 tgggatggct gtctgattga agcggtagaa aatgccgtct gaaacttatc cgcaggcagg
    11641 gttcggacgg catttttgat gcccaagcac ggtaaaacca gtaaaataaa cgctttcaaa
    11701 gatttcggag ccatcatgac cgtcaaaacc cgtttcgccc ccagccccac cggctacctg
    11761 cacattggcg gcgttcgcac cgccttgttt tcgtgggcgt ttgcccgcca tcataaaggc
    11821 gaatttttat tgcgcatcga agacaccgac ttggcgcgtt ctaccgccga atccgtcaac
    11881 atcatcctcg acggcatgaa atgggtcggc ctgaactacg acaacgccga caatgtcgtg
    11941 taccaaaccc gccgtttcga ccgctacaaa gaagtcattg ccgaactctt ggcaaaaggc
    12001 gatgcctact actgctattg cagcaaagaa gaattggaag cgatgcgcga gaaagccgaa
    12061 aaagaaggca cggcgactta cgaccgccgc tggcgaccgg aagcaggcaa aaccctgccc
    12121 gaaattcctg ccgacgtgca acccgtcgtc cgcttcaaaa cgcctttgga tggcgtaacc
    12181 aagtgggcgg acttggtcaa aggcgaaatt tccatcccca acgaagccct cgacgacctg
    12241 attatcgcac gcgccgacgg cacaccgacc tacaacttct gcgtcgtggt ggacgactac
    12301 gacatgggcg ttacccacgt tatccggggc gacgaccatg tgaacaacac cccgaaacaa
    12361 atcaacatct taaaagccat cggcgcaaac ctgcccgaat acggacacct gcccatgatt
    12421 ctcaacgaac aaggcaaaaa aatctccaaa cgcagcggcg ataccgtcgc cattaccgat
    12481 ttcggcgcaa tgggcatcct gcccgaagcc atgctcaact atctggcgcg cttgggctgg
    12541 gcgcacggcg acgacgagtt ctttacgatg gaacaattca tcgaatggtt tgatttgaaa
    12601 gacgtttccc cgtctccaag ccgtatggac ttgaaaaaac tctactggat caacggcgaa
    12661 cacatcaaaa tcaccaccaa cgaaaaactg gccgaaatgg tgaaaccgcg cctcgccctg
    12721 cgcgatatcc atgaaacaag caagcctgct ttggaagacg tgttggcatt ggtcaaagac
    12781 cgcgcccaag atttgaacgc cttggcagac gaatgtctgt atttctacaa aaaacaagtc
    12841 cctgccgaag ccgatgttgc caaacactgg gacgacgaag ccgccgcccg tatgctgcgc
    12901 tttgccgaac gcctcgaagg gctggaagac tggaatgccg aagccatcca cgacctcttc
    12961 aaacccttct gcgacgaaga aggcattaaa atgggcaaac tcggcatgcc cctgcgcctt
    13021 gccgtctgcg gtacggcgaa aacccccagc gtcgatgctg tgttggcatt aatcggcaaa
    13081 gaagaagtgt tgaaacgcat ccgcgcttaa gttatccaca ggcgtgggtg atgccgtctg
    13141 atgttggaat acggcttcag acggcattca aacggaagtt gaacatgaaa aaaatattgt
    13201 attttctgat gtttgttttt tctacaagcg tatgggccgg gtatgcggaa gacaatctgc
    13261 tcagcatcca atccggttat cgtgccttac tgcaaaaaca aaacaatctg gacggaaaaa
    13321 tcatcgggat gcagtcggat ttggaagatg cgcgcagacg gttgcaggcg gctcaggcag
    13381 acatcgcccg tttggaagcg gaaattcctg cagcaatggc gcaaaaagcc cggcaggctg
    13441 aagagctgag gcaaatcgga gtgcgtttgg accatgcttg gaatgcggtt tacggtgcag
    13501 ggggaacgaa agcgtcgggg aattgaccga tgaattccct ctttgtggac aatactgttt
    13561 tcattacacg gctgaaagcc gggcatatcg gcaggttggt tcaggcgttg tttgaggagt
    13621 ggcacggatt tgaaccgtgg tcttctgtgg ataagattcg tgcctattac ggcaggtgtt
    13681 tgaaggatga cgaactgccg ctggcttttg cggctgtgga tgatttcgga atcctgttgg
    13741 gttcggctgc ggtcaagcgg catgatatgg aaagttttcc acagtatgaa tattggttgg
    13801 gtgatgtctt tgttttacct gaatatcgcg gaaaaggcat tggcaggagg ctggtcgccc
    13861 actgcatagg cgcagcgcgt tcgctgggga taaagttctt gtatctttat acgcctgatg
    13921 tgcaaatatt ttatgaatca ttcggctggg cggttgtcgg gcgacatttc cataacggtg
    13981 aatgggttac ggttatgcgt ttggatatgg ataaggttta agccgaatgg acaaatgccg
    14041 tctgagctgt tcggacggca tttttatgtt tggaaacttc ttgtcagata atgtttatcc
    14101 acagattttt ccatatggtt tgatgctgtt ccgacaacgg aaattaagat acggatttgc
    14161 acaaatcttt cagacggtac agttctgaca atgcttcgcg cggagtcagt ctgtcgggat
    14221 cgagattttc caaggctgtt gccagtatac cttcaaaatg tttttcctct gctttgtcca
    14281 caaagttgcc cacattcggt tcatctcctt tttcagacgg catggtactg aaaatatcca
    14341 gttggggacg gttcgcggcg gcttggtttt ccagttcgtt caaatgcttt tgggcggatt
    14401 tcaatgcgcg tacaggcagg ccggcgagtt tggcgacggc aatgccgtag cttttaccgg
    14461 cgggacccgg ttggatttgg tgcaggaaaa cgatgtcctg tccctgttcg agcgcggaaa
    14521 ggtgcatatt gacggcggcg gcgtgggctt cgggcaggta ggtcagctcg aaatagtggg
    14581 tggcaaacag gctgaaggat ttgttttttt gcagcaggtg ttcggcaacg gcgtgcgcga
    14641 gggcgaggcc gtcgaaagtg gaagtaccac gtccgacttc gtccattaaa acaaggcttt
    14701 gttcggtggc gtgatgcagg atgtaggcgg tttcgctcat ttcgaccatg aaagtggagc
    14761 ggttggaggc gaggtcgtcc gatgcgccga tgcgggtgaa gatttgatcg atgggcccga
    14821 ttgtggcggc atcggcaggc acaaaacagc cggtgtgtgc caataaaaca atcagcgcga
    14881 cttggcgcat gtaggtggat ttgccgccca tattggggcc ggtgagcagc atgaggcggt
    14941 gtttgtggtc aaggtcggtg tggttggcgg tgaagtggcg tacctgctgt tcgacaacgg
    15001 gatggcggcc gttttcgatg tggataaccg gatagtcggc aaactcgggg cggacgaagt
    15061 tccgttcttt tgccaaggct gaaaatgtgg acaatacgtc cagcgcggcg gcggctttgg
    15121 cggctttttg aagctgcggc aatgccgtct gaaggttttt caatacgccg tcaaagagtt
    15181 gtttttctaa ggcgagggct tgctcttgag cagtcagcac tttgtcttca aaggctttca
    15241 gttccggcgt gatgaagcgt tcggcgtttt taagggtttg ccggcgttgg tagtcggcag
    15301 gtgcttgttc ggcttgggtt ttggacaatt caatgtaaaa gccgtgaacg cggttgaact
    15361 cgactttgag tgtggacaaa ccggtacgtt cgcgttcctt ggcttccaaa tccagcaaaa
    15421 attcgtcgcc atggttttga atgcggcgca attcgtccag ttcgggatga aaaccgtggt
    15481 tgatgacatt gccgtctttc agccagacgg aaggttcggg caaaatggct tggcggagct
    15541 gttcggctgt ggataggttt tccgggaaaa cggctttgag ggttcctaag agactgctgc
    15601 actcggcgga caattcgatt tcggacaggg caaacaggct gtcgcgcagg gcggcgaggt
    15661 cgcgcgggcg ggcgttaccc acggcaatac gggcggcgat gcgttcgatg tcggcaatgc
    15721 ttttcagacg gcactggagg ggtttgtatt ggctttccag cgcggcaacg gcttcttggc
    15781 gcgctcggat gtgggcgcgg ttgcgtaaag ggtggtgcag ccagagagcc aagaggcggc
    15841 tgcccatatg ggtagcgcaa aggtcgagcg tggacatcag ggtcggcgat tttttgccgg
    15901 agagggtttg cgtgatttcg agattgcggc gcgtggcggc atccataccg atatattggc
    15961 tgtcggtttc gagcgacagg ccgtccaaat gttgcggcat caggttttgc gtcagacgga
    16021 tatagttcaa cagtgcacct gccgcgccaa tcgcaacggc gtgttctttg ccgtccaaac
    16081 cgaagccgcg caaatcctgg cagccgaaat attccgtcag cagtttttcc cccgcgtcgg
    16141 cggcaaactg ccacgcgttc aggcgcgtaa cacccgatgc cgtctgaagt tgcggtgcgt
    16201 ttttactgtc aggcaacaga atttccgccg cctgcaggcg cgccagttcg tcgtccaatt
    16261 tatccacagt tgtcagcttg gttttgaatt cgccgctttg cagcgatgcc cacgccaaac
    16321 cgatgtattt tttgtcgggg gacacggcaa cgatgcggtt ggtttccttg tcttccagca
    16381 atgcggaatc ggtcagcgtg ccgggcgtta cgatgcgcac gactttgcgc tccaccggcc
    16441 ctttgcccgc gccgacttcg ccgacctgtt cgcaaatcgc cacgcttttg cccaacttga
    16501 ccaggcgcgc cagatattgt tcgacggcgt gaaacggcac gcctgccatt ttgaccggct
    16561 cgccatccac ctgtccgcgc gtggtcaggg taatatccaa aagttttgcc gcttctaccg
    16621 catcgtcgaa aaacatctcg taaaaatcgc ccatacggta aaacaccagt ttgtcggtat
    16681 gttgcgcttt gatgccgagg tattgctgca tcattgggga aacggcggat ttggacatgg
    16741 ggctgcctta tcattaggaa aacggtattg tagcaaaagg ttttgtcttc gacggaatga
    16801 gactgaccgt aagcctgtca ataaggttgt ggagacaact aggaaaacag caaagttacc
    16861 cacagaaata ttattaagtc aaaaaacatc cacgcttatc tgtcttatac agcaagtctt
    16921 aaactcaaac ttatttgaca aaaaggtaat gaaaatattg gatatttgct acttacgcac
    16981 aagcaggttg gcaatcatca aaatcatcat taaaatttta aattcaaata ttgatggtaa
    17041 aagttttgga aatctgtgca aacagtaaaa ttcatctata tcaaataagc atcaagtagt
    17101 tagatgtaat agtggaatgt aactttattt cagaatacaa tacaggtatt gaaatttcgt
    17161 ttcacagctt ggagtatata catgagcatt aaagtagcga ttaacggttt cggccgcatc
    17221 ggccgcctcg cattgcgtca aattgaaaaa gcccacgaca tcgaagtcgt cgccgtcaac
    17281 gacctgaccc ctgccgaaat gctgctgcac cttttcaaat acgacagcac acaaggccgt
    17341 ttccaaggta ctgccgaatt aaaagacgat gccatcgtgg taaacggcaa agagatcaaa
    17401 gttttcgcca atccgaatcc cgaagaattg ccttggggcg agttgggtgt ggatgtcgtc
    17461 ctcgaatgta ccggcttctt taccaataaa accaaagccg aagcccacat ccgtgccggc
    17521 gcacgcaaag tcgtgatttc cgcacctggc ggcaatgatg tgaaaaccgt cgtatatggc
    17581 gtgaaccaaa acattttgga tggcagcgaa accgtcattt ccgccgcttc ctgcaccacc
    17641 aactgccttg ccccgatggc ggcagtcctg caaaaagagt tcggcgtagt cgaaggtctg
    17701 atgaccacca tccacgccta caccggcgac caaaacaccc ttgacgcgcc gcaccgcaaa
    17761 ggcgatttgc gccgcgcccg agccgccgcg ctcaacatcg tgcccaacag caccggtgcc
    17821 gccaaggcca tcggtctggt tatccccgaa ttgaacggca aactcgacgg ctccgcccaa
    17881 cgcgtccctg ttgccaccgg ctcgctgacc gaattggttt ccatcctcga acgccctgta
    17941 accaaagaag aaatcaacgc cgcgatgaaa gccgccgcca gcgaatctta cggctacaac
    18001 gaagatcaaa ttgtttcttc cgacgttgtc ggcatcgaat acggttcgct cttcgatgcg
    18061 acccaaaccc gcgtgatgac cgtgggcggt aaacaactgg tgaaaaccgt tgcctggtac
    18121 gacaacgaaa tgtcctatac ctgccaactc gtccgcactt tggaatactt tgcaggcaaa
    18181 atctaaggct tgaacaaacc tgtggataag taaatgccgt ctgaacacat tgcgttcaga
    18241 cggcattgtt tatctgtata gtggattaaa tttaaaccag tacggcgtta cctcgccttg
    18301 ccgtactatt tgtactgtct gcggcttcgt tgccttgtcc tgatttaaat ttaatccgct
    18361 ataacttgat attgcggctt atggcgaaca aacaacagcc gtttgttcaa ctgctatgcg
    18421 gcaaaaccgg atttttgccc gatttaaagc ggggttgggg aaagatcaat aacggttgca
    18481 cggttttcct cgatttgaaa tctgacttcg taatctgcaa tattcatcac ataaatccgt
    18541 tcgggaatat tctgataggc ggggcgcgga tcttgggcaa tgctttggct gataaggttt
    18601 ttggtgtttg cagataaatt ttccgcgccg atgttttcct gccaaacgac ttccaactct
    18661 acgggtttgc cgctgacgaa accagatgcg gcatcgggtt tggattcgac aaaggggata
    18721 taaggcttga tgtccacaat cggcgtgccg tcgagcaggt ctgcgccgct gcaataaagg
    18781 cggacgggtt tgccggtttc gatgcggttg agttttaaga gtgatagtcc gagatggttg
    18841 gggcggtggg ggctgcgcgt ggcgaatacg cccatttttt gtttgccgcc gagccgtggc
    18901 gggcgcacca tttgtgccca gccttcatcc aatacgccgt ggaagataaa acttatccac
    18961 acataatcga aatcttccag cccgcgcacg ctgtctgcgg taaattcggg attcagctcg
    19021 atgcatactt ctgcggcaga ggctaaaccg ggctggcggg cgatgccgaa tttctgtttg
    19081 tagggcgatc gggcggtgcc gatgggggta atggtataag tcataaggtt gtggataagt
    19141 ttttttggaa aaaccttatc ttatcacaat cggttttacc gtttttgggc agtaatttgt
    19201 tataatacag ggcattttaa tcgcgttgaa ataatgagat gattgtttcc attgatgttg
    19261 atgcacaaaa aacgtttacg ccactgtgtc ctgacgaact gcccgtgaac gaggggcatt
    19321 tgattgtcga ggagttgaat gctcaagccg ctttggcgga tttgcgcgtg atgacgaaag
    19381 atgcgcatca tatggtagcg aaatggcttg tggataaccc tgttgatatg ttgaagccga
    19441 caggtttgcc tgatgcggat ttgacttggg tggctcatgc gatggtcggt acgcgcggct
    19501 atgaattatt ggacggactg ccttctgcca aagaatatga ttattgcgtt tggaaaggtg
    19561 ttgatcctga attgcatcct tacggcgcgt gttttcacga tattgaggaa aaactaagca
    19621 cagggctgat tgaatggctg cgttgtcaaa atacggatac ggttattatc ggtgggttag
    19681 ctacggatta ttgtgttaaa acaacggttt tgcagttact taaaggtggt aattggcagg
    19741 ttatcgtcaa tgaagcggct tgtcggggca ttgcgccgga taccattgaa acggcatggc
    19801 gggaaatgag ttctgccggt gcagtcatct taaaaaacgc tgaagaaatt aaaaaatata
    19861 ttaataatca ataagttaaa attgtttcac gtgaaacata cttcttaaaa tatgaaaatt
    19921 ttgcttgtcc gtttgtctag tatgggcgat ttaattcaca ctttgcccgc aattgaggat
    19981 ttggcgcgac aatgccctga tgtagaactc cattggttgt gtgaagctgg atttacagat
    20041 attgcgcgcc tgcatccgtt tgtgaaaaaa atccatgtga tgaaatggcg gcaatggcgc
    20101 aaacatctct ttcaggctga aacttggcgg gaaatgggtc atctgaaaca gactttgcgg
    20161 caggaagcat ttaattttgt attggacagt caaggtctga ttaaaagcgc gtgtttcgct
    20221 aaaatggcga aatcccctat ttatggtttg gataaaaaca gtgcgcgcga gggatgggct
    20281 gctttggcgt atgtaaaaac atacgctgta ccgaagggaa aaaatgccgt ttggcgcaac
    20341 cgtgaactgt ttgcccaagt atttgggtat gcgatgccgg aaacgcaggt atttggcttg
    20401 actgttcctg aagcaggccg tctgaaaaat ttagcgcagc cgtattatgc ggctttgcat
    20461 gcgaccagtc gggacagtaa gttatggcct gtggaaaact ggcggtcgtt gctgcaaaag
    20521 ttgaatgaag aacagcaatg caatatttac ctgccttggg gaaatgaaga tgaaaaaacg
    20581 cgtgccaaac agattgcaga cggactgccg tttgctattg tgtgcgacaa aataaattta
    20641 ttgcaggcag cgtatctgct gaaatacgcg gtcggaattg tcggcgtgga taccggtttg
    20701 ctgcatttgg caaatgcctt ggaaaaacct gtggtcggca tttataccga taccgatccg
    20761 attaaaacag gcgttcaggt ttcgccaatt gcaaaaaatt tgggcaatat cgggcagatt
    20821 ccgaccgcag atttggttta tcaaacgttg atggattgtg ttgcagcaga caaaggctaa
    20881 agggttgtct gaaactgata tagcacctga tatttttcag tttgttaagc ataagaatgt
    20941 atatctacca gcctgagtaa atgctttaaa aatggtataa ttggcaagca ttatccgaaa
    21001 gatttttgaa tgaattcgtt tttaaggaaa acggatttgg ttttataggg aagaaggttt
    21061 ggtttcgctg gatggtgaaa gcagcgttct ttttaggagg gttcaatgaa agcactggtc
    21121 gcagtaaagc gcgtagtgga ctacaacgtc aaagttcgtg taaaagccga tggttcggat
    21181 gtggatatcg gcaatgtcaa aatgtcgatg aatccgtttg acgaaatcgc tgtggaagaa
    21241 gctgtccgtt tgaaagaagc cggaaaagta agcgaaatcg tagcggtttc tttgggtgag
    21301 aaaaaatgcg aagaaacctt gcgtacagct ttggcgatgg gtgccgaccg tgccattcat
    21361 gttgaaaccg atactaaact ggagtctctg gccgttgcta agttgctgaa agccgttgcg
    21421 gacaaagaaa atccgcaaat tttctttttg ggcaaacaag cgattgatga tgatgccaat
    21481 caagtggcgc aaatgctggc agctttgctg aatgcggcgc aaggtacgtt tgcttcaaaa
    21541 gtacacattg aatgtggtga agtacagatt gtgcgcgaaa ttgatggcgg cgaagaaacc
    21601 atagcattaa aactgcctgc tgttatcagt gctgatttgc gtttgaacga gccgcgcttt
    21661 gtcaaactcc ccaatattat ggcggcaaag aaaaaacctt tggaaaaact gactcctgac
    21721 gatttggttg ccgacatttc acctcgtttg aaaacggtga aatttgccga acctaaggcg
    21781 cgtcaggcag gcgtaaaagt agcaagcgtt gccgaattgg ttgaaaaatt gaaaaacgaa
    21841 gccaaagtga tttgaggagg ctgaaatgag tgtattgatt attgtcgaac acgacaacaa
    21901 acagttgaat cctaccactt tgcatgctgt taccgctgcc gctaagctgg gtaaaatcga
    21961 tttattggtt gccggaagcg gtgcatctgc tgtagtagag ttcgcaaaac aagtggcggg
    22021 tgtggaaaaa gttttggttg cagatgctgc ccattatgcc gaaggtttgg ctgaagagtt
    22081 ggctccgctg gttgtcaaat tggcagcgga ttactgctat gttgcagcaa cggcaactac
    22141 attcggtaaa aaccttttgc ctcgcgtagc agccttatta gacgtaccgc aaatttctga
    22201 tttgaccgaa attgtggata acacgacttt tgtgcgcccc atttatgcgg gtaatgcatt
    22261 tgaaaccgtg caagctgatt cagaaaaatt ggtgctgacc ttccgtgcga cggtttttga
    22321 cgcagtggcg gcgcaaggtg gtaatgctga ggtaattaat gttgaagcaa cccctgccca
    22381 aaacctgagc cgttttgtta accgtcagct ttcccattcc gatcgtcctg aactgactca
    22441 ggcaaaagtg attgtttccg gtggtcgtgc gttgggtagt gcggaaaaat tcaatgaagt
    22501 gctgacaccg ttggcggatg ttttaggtgc ggcaatcggt gcatcccgtg cggcggttga
    22561 tgccgaatat gcgccaaacg atgctcaagt cggacaaacc ggtaaagtgg ttgcgccgca
    22621 actctatttt gcaatcggta tttcaggtgc gattcaacac gttgccggta tgcaagacag
    22681 taaggtgatt gttgcaatca ataaagatgc tgatgcgccg attttcaatg tagccgatta
    22741 cggattggtt ggcgatttat ttgaaatcgt tccacaatta acagaagcat tgaaaaatta
    22801 atgtttcacg tgaaacgttc agacgacctt gttattaatg aggtcgtctg aaaacatttc
    22861 agcatgatta cgacttataa aaccattacc tccttgacgc aggctgagtt taaagataaa
    22921 ggcagccgtt ttattgcatt tgcctatccg attcggacat tggctgatgt gaaaaaatac
    22981 ctcgatccgc taaaggaaga gcatcataaa gcacgacact ggtgctatgc ctatcgtttg
    23041 ggtgtggatg gtgtgcaatt tcgtgccaac gatgatggag agccatcagg aagtgctgga
    23101 cgaccaattt tgggacagat tgattcggtg ggtattaccg acgttttggt tgtggtcgtc
    23161 cgctatttcg gtggtacttt gttaggtgtt ccaggtctga tacatgcgta caaagaggcg
    23221 acggctcaag cgttggcagt tgcagaagta gttgaaaaga atattgaaaa aactgtttgg
    23281 ctgaaatgtg aatatccgtt tttgaatgaa gcgatacgca ttgccaaaca atatcaggcg
    23341 gatatattgg agcgggattt gcagttggat tgccgattga cggtcagttt gtcgttggta
    23401 aattatgagg cctgtgttgc ggcttggaag aatactcggc agattgaagt aaatacggaa
    23461 aagccttttg aatgaaaggt cgtctgaaat gctttgtgat ggtatttcag acgacctctt
    23521 cgctgactaa tcttcttgtg tatatttgtc taaggcatcg acgctggagc gcaaatataa
    23581 ccaagccaat tgatggaatt cgcccagcgc gttccatagc gcatcttcac tcataatgct
    23641 gtattcgcct tccgtactca aatccagttc taccccgtct tcaatagcct gatgacacgc
    23701 ctgatactcg tgggtataga aattgtccat gtcgcgaatt aaagtgacga catatttcca
    23761 acggcggatg tcttcttcca gttgcggtag taaatgacac cattcgcggt atttgctttg
    23821 gatttcatca atacgttttt gcatggttgc ttgtcctttt ttaatttatg gataagttga
    23881 ataggttgca gagtggaatg aagcggtctt ttgtttgatt ctactaaaat ttcttcagat
    23941 gggataaaag tccctttgta tcgagttttc gttaaaagta catatttgtc aaggagatgg
    24001 catgaaactg ctggttatcg gtaatggcgg tcgcgaacac gcgctggctt ggaaattggc
    24061 gcagtcgcct aaagtggaaa cagtatttgt tgcgcctggt aatgccggta cagcgattga
    24121 acccaagttg caaaacatcg acttgactgc gcatcaggat ttgattgaat tctgtcgtaa
    24181 agaaaatatt gtttttaccg tcgtcggccc tgaagcgcct ttggcggcag gtgttgtgga
    24241 tgatttccgt gccgcaggac taaaaatatt cggtccgaca caatatgcgg cgcagttgga
    24301 aagttccaaa gatttcgcca aagcatttat ggcgaaatac aacattccga ccgcgcaata
    24361 tcaaaccttt gaaaacgccg atgccgcaca tgattacgtc aatcagaaag gtgcgcctat
    24421 cgttatcaaa gccgatggtt tggcggcagg taaaggcgtg attgtggcga tgactttgga
    24481 tgaagcgcat gctgcgattg atgatatgct gctggacaac aaaatgggca atgccggcgc
    24541 gcgtgttgtg attgaagatt tcctgcaagg cgaagaagcg agttttatcg tcatggttga
    24601 tggcaataat gtactgccta tggcaactag ccaagaccac aagcgtcttt tagacggcga
    24661 caaaggtctg aatacgggcg gtatgggtgc gtacagccct gcgcctgtgg taacgcctgt
    24721 tgtgtacgaa cgcgcgatga atgaaattat tttgccgacc gtagcagggg tgaaagcgga
    24781 ggggtatgag ttcaccggtt tcctgtacgc aggtttgatg attgatcaaa gcggtgcacc
    24841 ctatacgatt gagtttaact gccgtttcgg cgatcctgaa acccagccga ttatgagccg
    24901 actgaacagc gacttatcgg atttggttga agcggcaata gacggcaaac tcgatagcgt
    24961 aaccgcggaa tggagcccgc aaactgccgt gggcgtggta ctggcggcgc aaaattaccc
    25021 cgaaacgccc aaaaaaggcg atgttatttc cggtctggat gctgccaacc aagtaggcaa
    25081 agttttccat gcaggtacaa cggcaaacga gaaaggtgac gtattgacca acggcggacg
    25141 cgtattgtgt gttgtgggat tgggcgataa tgtggcgcaa gcgaaagcta aggcatatgg
    25201 cgcgttggaa aaaatcagtt tcgacgggat gcaataccgt aaagacattg ccgataaagc
    25261 gattaaccgt taattagaaa gaggatgatg ccgtctgaag ccaagtatga ctgatgtgct
    25321 tcagacggta ttttatatta tgttatttcc gagaattatc gcagcatcag cgcaacgtaa
    25381 gttaagcgtt tatttgaaaa aaggcggttt ggtcgcttat ccgaccgaat cttgttatgg
    25441 cttggggtgt cttccgacat tagccaaagc actcggtaag ttggcgcatt tgaaaaaaag
    25501 accgcaacac aagggtatga ttgtcatcgg gaatcaattt gagcaattgc agcctttact
    25561 tcaaatgccg tctgaaaatc ttcaggatat gcttagaaaa gaatggcctg cccctaaaac
    25621 gttcctatta tccgccaaat cctgtgtctt acccgagtta cgcgggaaac agcgcagcaa
    25681 gttggcggtc agggttcccg cccatgtagg tgcgcgccgc ttgtgtcagg ctttgcaaac
    25741 gcctttggtc tcgacttcgt gcaaccgcgc aggcaaacgc gcgtgtcgga cggagaggga
    25801 agtcagaagg caattcggac gcgatgtgtg gattgttggt gggagaatcg gcaggcagaa
    25861 atcgccgagt cagattattg acggggaaac aggtaaacgt ttgcgttaga tatgggtact
    25921 tgaatgaaaa cttttttgca tacacaaaga aaaaattt
//
LOCUS       DAXHKX010000009        24835 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHKX010000009 DAXHKX010000000
VERSION     DAXHKX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.009"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(144..234)
                     /locus_tag="ACFMWZ_000353"
     tRNA            complement(144..234)
                     /locus_tag="ACFMWZ_000353"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(273..359)
                     /locus_tag="ACFMWZ_000354"
     tRNA            complement(273..359)
                     /locus_tag="ACFMWZ_000354"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:cag)
     gene            complement(395..481)
                     /locus_tag="ACFMWZ_000355"
     tRNA            complement(395..481)
                     /locus_tag="ACFMWZ_000355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Leu,seq:cag)
     gene            570..2954
                     /gene="rnr"
                     /locus_tag="ACFMWZ_000356"
     CDS             570..2954
                     /gene="rnr"
                     /locus_tag="ACFMWZ_000356"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC5934373.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAILNGQNYEPGKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIEVQKVKKRKGKS"
     gene            2954..3223
                     /locus_tag="ACFMWZ_000357"
     CDS             2954..3223
                     /locus_tag="ACFMWZ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934374.1"
                     /translation="MLTGRLGEGRAGHTFPKPPPDFLIWRPAHHFRPIETPVLKDCVY
                     FHRRFKGRRYSADWAQTAFRRHFHSFGVSVRIALPVRAISPCGLP"
     gene            complement(3298..4761)
                     /gene="guaB"
                     /locus_tag="ACFMWZ_000358"
     CDS             complement(3298..4761)
                     /gene="guaB"
                     /locus_tag="ACFMWZ_000358"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC5934375.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4908..7466)
                     /gene="glnD"
                     /locus_tag="ACFMWZ_000359"
     CDS             complement(4908..7466)
                     /gene="glnD"
                     /locus_tag="ACFMWZ_000359"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC5934376.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADELAKKMNAQRNVPA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATNLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSTVAQKEDIQDPSVLDAFCKRVQTHERLSALYFLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NNFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            complement(7515..7835)
                     /locus_tag="ACFMWZ_000360"
     CDS             complement(7515..7835)
                     /locus_tag="ACFMWZ_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC5934377.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            7850..8119
                     /locus_tag="ACFMWZ_000361"
     CDS             7850..8119
                     /locus_tag="ACFMWZ_000361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934378.1"
                     /translation="MVKVKHLLLRILLHIKAKKRGGGSHTQTHTHQEERGNIKTDWET
                     ANKQSRFHIGIIKQKPLSSIDSNQLRFDSQTGKEKRHTRLYGYAV"
     gene            complement(8159..8632)
                     /gene="bfr"
                     /locus_tag="ACFMWZ_000362"
     CDS             complement(8159..8632)
                     /gene="bfr"
                     /locus_tag="ACFMWZ_000362"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC5934379.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLEKEKEKHEALI
                     AAISVAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8660..9124)
                     /gene="bfr"
                     /locus_tag="ACFMWZ_000363"
     CDS             complement(8660..9124)
                     /gene="bfr"
                     /locus_tag="ACFMWZ_000363"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC5934380.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9588..10571)
                     /gene="lipA"
                     /locus_tag="ACFMWZ_000364"
     CDS             complement(9588..10571)
                     /gene="lipA"
                     /locus_tag="ACFMWZ_000364"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC5934381.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10564..11187)
                     /gene="lipB"
                     /locus_tag="ACFMWZ_000365"
     CDS             complement(10564..11187)
                     /gene="lipB"
                     /locus_tag="ACFMWZ_000365"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC5934382.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11189..11464)
                     /locus_tag="ACFMWZ_000366"
     CDS             complement(11189..11464)
                     /locus_tag="ACFMWZ_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC5934383.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11649..12719
                     /locus_tag="ACFMWZ_000367"
     CDS             11649..12719
                     /locus_tag="ACFMWZ_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC5934384.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(12826..13773)
                     /locus_tag="ACFMWZ_000368"
     CDS             complement(12826..13773)
                     /locus_tag="ACFMWZ_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC5934385.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(13788..14195)
                     /locus_tag="ACFMWZ_000369"
     CDS             complement(13788..14195)
                     /locus_tag="ACFMWZ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC5934386.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            14276..14935
                     /gene="ung"
                     /locus_tag="ACFMWZ_000370"
     CDS             14276..14935
                     /gene="ung"
                     /locus_tag="ACFMWZ_000370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC5934387.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            14932..15141
                     /locus_tag="ACFMWZ_000371"
     CDS             14932..15141
                     /locus_tag="ACFMWZ_000371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC5934388.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            15083..15685
                     /locus_tag="ACFMWZ_000372"
     CDS             15083..15685
                     /locus_tag="ACFMWZ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC5934389.1"
                     /translation="MRAFIKNYVLTLLFLKYVSDKHKYGGGMIELHAGTTFDDIVKLK
                     NTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEMIDRLSRLVGIFKKLN
                     LSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRIMAKIIGISADCRPAP
                     ALMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            15598..16231
                     /locus_tag="ACFMWZ_000373"
                     /pseudo
     CDS             15598..16231
                     /locus_tag="ACFMWZ_000373"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            16219..16533
                     /locus_tag="ACFMWZ_000374"
     CDS             16219..16533
                     /locus_tag="ACFMWZ_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HFC5934390.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            16575..17519
                     /locus_tag="ACFMWZ_000375"
     CDS             16575..17519
                     /locus_tag="ACFMWZ_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC5934391.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            17670..17795
                     /locus_tag="ACFMWZ_000376"
     CDS             17670..17795
                     /locus_tag="ACFMWZ_000376"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934392.1"
                     /translation="MLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT"
     gene            17900..18787
                     /locus_tag="ACFMWZ_000377"
     CDS             17900..18787
                     /locus_tag="ACFMWZ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC5934393.1"
                     /translation="MSAPVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQ
                     PSLSKYYPISHRAPNGIVELLLGENTEEIIRSTISNTVFPNLDIVISNNISAFVETEV
                     TSRPDRAFLLRSKLHSQYIQNNYDVVLIDTQGAVGPLLDTAAFAATCLLCPIMPEVLS
                     AREFLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKDFFSNTLDD
                     RRKLLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKN
                     LRGSCFNDMASLLQEEVQS"
     gene            18784..20304
                     /locus_tag="ACFMWZ_000378"
     CDS             18784..20304
                     /locus_tag="ACFMWZ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFC5934394.1"
                     /translation="MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEID
                     FFDKNPRRLHDVSSYAAIKESIRSSGIQQPVHVTQRPGESKYVLAQGGNTRLKILCEL
                     LEETGDNRFRMMPCIYIHYSDETTLQVAHLVENELRAEMCFWDKACKYAEIRDIFQDE
                     SDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHAKVEEIKKL
                     HSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSEA
                     FSETENSSDKDKIETAVKQDVNLHQKEVSENNTESEDSDMEFVPAKVNGTKADAEDEP
                     KEAELITLEQTSKKLFSAVRKLLAKVSLSDCFKIHKDFPYGWYVEYPAFEHISASSAG
                     NAIYAIDVLHEEAGNVFTFLWKLSGIDTFMFDVGDSSNPLLTLKSDSKLRIAYEDPDK
                     YDEYTNCGIGNKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKTGAKSEE"
     gene            20294..20899
                     /locus_tag="ACFMWZ_000379"
     CDS             20294..20899
                     /locus_tag="ACFMWZ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934395.1"
                     /translation="MRSKPLISNPIYRLFNTDPVYTNLALTRLASAPNTPRESSYNAL
                     VAREHEDVLNTLKRMNPKRIEKLASMCPDVVTYSIDLKVLKSKITEMEHYEQRFMEQM
                     QRLKWMLEHKASNLMIMNLCSLISTAEIKKLRIEMNIPVVKGRIVMPSIDVRVRVLKA
                     WSKSEKETDLFIRYQLLIDEFPDYSLAQLHSIINDVKFFGV"
     gene            20908..21651
                     /locus_tag="ACFMWZ_000380"
     CDS             20908..21651
                     /locus_tag="ACFMWZ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934396.1"
                     /translation="MDFSTSYDYETGFVPSKNMATDNFIRSLVALWEEPATYVRQFAQ
                     AVGSVSEGYFLSWFVEKCLPGEWVSLNNDLLFNEAGITAKQWRNIRNSLLQKGILLNR
                     RTVAPTESQFTLNDALFEDLVSKHGKSDVLDMVSPPISINKLHLKTLADRGLAFVSIL
                     YLSFLQSEAEYQDLSKRGDWSPWTHAAEKHVFYSTMLSRRQQERSIAELTSFGLIESC
                     VDGMPAARRCRYSLKMLADLTSEYLNGCV"
     gene            21651..23168
                     /locus_tag="ACFMWZ_000381"
     CDS             21651..23168
                     /locus_tag="ACFMWZ_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934397.1"
                     /translation="MVTATKLPPSKVFNSELTQCWQALQGSRRVAIYRCLIDITGSLK
                     TAAMLSQLIYWTRVSKEVAERDGWIFKSIAQMEAETGLTVREQRTCKNKLLETNLIQT
                     CRKGVGAALAIKVNLDAIAELIAKSSGTDDQLALTLADLQNTSSLYFRKHFSKRIAYH
                     RDLVSITGCINSAVLLSCVLNDAVGLVSQNPHLQRHAFATLTATDWEERTSLSYKSQL
                     TARNRLKNLNLIYERNFLASRRIFTLVNGHDIVISIKKLLAGKQDDGFSPYNSKKREI
                     LSLAERAKCDWRKGPNGLDKGFQSDSEGDAPLFPSMTSGSVERKYSDVSNWNFKKGHS
                     GKFTSVDWESSDLSNRQIIYYINYKGVYNYNYMTVEPNKSRSSEQDHVVVVDDVDLIY
                     PKLFNVQMRQQADLIFTKHLSKSSWALRQEILDEIQGQKKEVTSPLGLLINLCRCASQ
                     GNFTALVAPQVRFQREQDQKLQAYLEKQNNQEQDSLDTMDVELARNNLRKFYSKK"
     gene            23360..24409
                     /locus_tag="ACFMWZ_000382"
     CDS             23360..24409
                     /locus_tag="ACFMWZ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFC5934398.1"
                     /translation="MEFAKQRYIIKPGAIHVAQQFDLKVTTKQSPFSDGYDMARAALA
                     LGGKVNQKLTEDDEQFQQWEEFKAHELLLKQHLQKETGRKHHLVWGEYEILSSENDRF
                     KNIGSLTSSGDDGFFMHTKQGIRMWEGNNNKKNGPRWPGIRYGLTLSNELFGMAMSDN
                     PYAQSRLLQIEKQMSEIEEFFETVKKQVHAQIDSLAAAGMKITLIQNNNPVHIRLNVL
                     RAYGFKLVKLLRDYDSFVCAVKTLNIKGMMANKQCNATLYNGGRMMRKLLNDLYIAVM
                     ETRAIKSIRRDSLLDPEQLSKLKSAVEQEILPRIPLSVWVYESQPSLVYIQRKMNQED
                     LNKLVDIVRDNGLSG"
     gene            24496..>24835
                     /locus_tag="ACFMWZ_000383"
     CDS             24496..>24835
                     /locus_tag="ACFMWZ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934399.1"
                     /translation="MNTMNTQISGMKKYLKFWEYKRLLRVRGIEDLEKEIKLKLVNFE
                     LLIDEAQSFHEACQDLNLIYPIVREHLKLSNKSLAGLDLKKYYIPNMIFFKNVVSSSG
                     RMSRKKFFKWT"
BASE COUNT         6184 a         5635 c         6500 g         6516 t
ORIGIN      
        1 taccgcaaaa cataccataa aaaaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggga taatttatta tttggtgccc gggagaagac tcgaacttcc
      301 acacccatga ggataccagc acctgaagct ggcgcgtcta ccaattccgc cacccgggca
      361 atctaaatat tgaaataaag caaagcattt gatttggtgc ccgggagaag actcgaactt
      421 ccacacccgt gaggatacta gcacctgaag ctagcgcgtc taccaattcc gccacccggg
      481 ctttgcttta tttgctgttt tgcggtacag tgtcctgccg caaagaagtc gctattatat
      541 agttcataaa gagaatgtca acagtccaaa tgaataaaaa tattaaatct ttaaatttac
      601 gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta tgaacatcct ttgcccagtc
      661 gggaatggat aatcgaattg ttggagcgca aaggtgtgcc ttcaaaaatc gaatcgcttg
      721 cgcgcgagct gtcgattacg gaagacgagt atgtcttttt cgaacgccgt ctgaaggcga
      781 tggcgcggga cggtcaggtt ttaatcaacc gtcggggcgc ggtttgcgcg gcggacaaat
      841 tggatttggt caaatgccgc gtcgaggcgc ataaggacgg tttcggtttt gccgtgccgc
      901 tcacgcccgc caaagacggt gattttgttt tgtatgaacg ccagatgcgc ggcattatgc
      961 acggcgatat tgtcactgtt cgtcctgccg gcatcgaccg taggggccgc cgcgaaggga
     1021 cagttctgga tattgtcgaa cgcgcgcaaa gcaaagtggt cggccgtttc tatatggata
     1081 ggggcgtggc gattttggag ccggaagaca agcgtctgaa ccaaagcatc gtattggaac
     1141 cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt catcgtcggc gaaattgagg
     1201 tttatcctga gcaaaaccgg ccggcagtgg caaaaatcat cgaagttttg ggcgattatg
     1261 ccgacagcgg catggagatt gaaattgccg tgcgcaagca tcatttgccg caccaattca
     1321 gtgaagcgtg tgccaaagcc gtgaaaaaaa ttcccgacca tgtacgcaaa agcgatttga
     1381 aaggccgcgt cgatttgcgc gacctgcctt tggtaacgat tgacggcgaa acggctcgag
     1441 attttgacga tgcggtgttt gccgaaaaaa tcggacgcaa ttaccgtctg gtcgtggcga
     1501 ttgccgatgt cagccattat gtccgccccg atgacgctat cgacacggac gctcaggaac
     1561 gcagcaccag tgtttacttc ccgcgccgcg tgattcccat gttgccggaa aacctgtcca
     1621 acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg tatggtgtgc gatatggtca
     1681 tcacttacgc gggcaatatt aaagaatacc gcttctaccc cgccgtgatg cgctctcatg
     1741 cccgcctgac ctacaaccaa gtttggaaat ggctttcagg cggcatcggg catccgttca
     1801 aagcccaaat cgacacgctt tacaaactct tcaaaatcct tcagaaaaag cgtttcgaac
     1861 gcggggcggt ggagtttgac agcatcgaaa cccaaatgct tttcgacgac aacggtaaaa
     1921 ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa gctgattgaa gaatgtatgt
     1981 tggcggcaaa cgtttgcgca gcggattttc tgttgaaaaa caagcatacc gcattgttcc
     2041 gcaaccattt ggggcccacg cccgaaaaac tcgccgcctt gcgcgagcag ctcggtctgt
     2101 tggggcttca acttggcggc ggcgacaacc cgtcgccgaa agactatgcc gcgcttgtcg
     2161 aacaattcaa aggcagacct gatgccgaat tgctgcaagt catgatgttg cgctccatgc
     2221 agcaggcggt ttacgaaccg cattgcgaag ggcatttcgg tttggcttat gaagcatatg
     2281 cccattttac ttcgcctatt cgccgttatc ctgatttaac cgtccaccgc gccatcaagg
     2341 cgattttaaa tggacaaaat tatgaaccgg gtaaaagctg gcaggctttg ggcgtgcata
     2401 cctcgttctg tgagcgccgt gccgacgacg ccagccgcga cgtggaaaac tggctgaaaa
     2461 cctattatat gcgcgataag gtcggcgaag tattcgaagg taaaatctcc ggcatgacca
     2521 gttttggtat ctttgtaaca ctggacggca tccacattga cggcttggtg catatcagcg
     2581 atttgggcga agactatttc aacttccgcc ccgaaatcat ggcaatcgaa ggcgaacgca
     2641 gcggcatccg tttcaacatg ggggacaggg ttgccgtccg ggtcgcccgt gccgatttgg
     2701 atgatggaaa aatcgatttt gtcctgattg ccgaggggag cggcaggggg cggaaagtta
     2761 aatcatccgc gtctgccaaa ccggcaggga cggcggggaa agggaagccg aaaaccgccg
     2821 ccgagaaaaa aacagcccga ggcggcaaag taaggggaag gggcgcgtct gccgccgcag
     2881 aatcggggaa aaaggcaaag aaaccggttc cgattgaagt acaaaaggta aaaaaacgga
     2941 aaggcaaatc ataatgctga cggggcggct tggggagggc agggcagggc atactttccc
     3001 gaagccgccg cccgattttt tgatatggcg gccggcgcat catttcaggc caatcgaaac
     3061 gcccgtattg aaagattgcg tttatttcca ccgccgtttt aaaggccggc ggtattcggc
     3121 agactgggcg caaacggcgt tcagacggca ttttcattct ttcggcgtgt ccgtccgaat
     3181 tgctttgccc gtccgcgcaa tcagcccgtg cggcttgcct tgaacggaca aaaaatgccg
     3241 tctgaaaccc gaaaatcagg tttcagacgg cattttgcct tgaaaaggct gttcaaatca
     3301 gcgatggtag ttcggtgctt ctttggtaat ttgaacgtcg tgaacgtgcg attcgctcat
     3361 acctgcggaa gtgatttcca caaattctgc tttttcgtgc atttcggcaa tattggcgca
     3421 acccaaatac cccatgctgg agcgcagtcc gccggtcagt tggtggatga tgttcacaat
     3481 cgggcctttg tagggaacgc ggccttcgat gccttcgggg acgtatttgt cggtgctgtc
     3541 ggttttgtct tggaagtagc ggtcggcaga accttggctc atcgcgccca aggaacccat
     3601 accgcgatag gatttgtacg agcggccttg gtagagttcg atttcgcccg gcgcttcttc
     3661 cgtgcctgca aacataccgc cgagcatgac gctgtacgcg cctgcggcga gggctttggc
     3721 gatgtcgccg gagaagcgga tgccgccgtc ggcaatcagc ggaacgcccg tgcctttgag
     3781 ggcttcggca acgttgtgaa tggcggtcag ttgcggcacg ccgacacctg ccacgatacg
     3841 ggtggtgcaa atcgatcccg gaccgatacc gactttgacg gcatccgcgc cggcggcgac
     3901 caaatccaat gcggctttgg cagtggcgat gttgccgccg atgacttgga tgtgcggata
     3961 ggtttctttc acccatttca cgcggtcaat cacgccttgg ctgtgcccgt gggcagtatc
     4021 gacgacaatc acgtccacgc cggcttcgac caaggctttg acgcgctctt cggtatcgcc
     4081 gccggtgccg actgccgcac cgacgcgcag acggccttcg gagtctttgt tggcgttggg
     4141 aaactcggtg gtttttaaaa tatctttaac ggtaatcaga cctttgagtt cgtctttttc
     4201 gttcagaacc aaaacgcgct cgactttgtg cgtgtgcatc agttcgcgcg cttcgtctat
     4261 gcttgtgcct tcggggacgg taaccagacg ttcgcgcggg gtcataatgg cggaaacggg
     4321 caaatcgacg cggttttcaa aacgcaggtc gcggttggtt acgatgccga cgactttgcc
     4381 gttttcaacg acgggcaggc cggacatttt gcgtttgcgc tgcgcacgca tttccaagac
     4441 ttcgcggatg agcgtcgtcg gtgcaacggt tacggggtct ttgaccacgc cgctttcgtg
     4501 gcgtttcact ttggaaacgg cgcgcgcctg catttcgggc ggcatgtttt tatggatgat
     4561 gccgatgccg ccttcctgtg ccatcgaaat ggcgaggcgc gcctcggtaa cagtgtccat
     4621 cgcagcggaa agcaggggga ggttgagtgt gatttcgcgg gtgagcttgg tttgaagttt
     4681 aacgtctcgc ggcagcacgg tcgaatgtgc gggaaccaac aaaacatcgt cgaaagtata
     4741 ggctttttct acgatacgca taatgctcgg tctttcagtt tgtgcaagat gcacggcatt
     4801 atagcacgtt accggcggct tgacagttta tattgtttgc aatatccccc gccttcaggc
     4861 ggcatacggc gcggcggcgg aatgccgtct gaaaaccggc agggtgccta tgccaactgt
     4921 tccagcaaag cctgtttcaa tgaggactgg atttttgggt ttttcaggtc ggggctaaaa
     4981 acggtaaaac tgtcttcggc gcgttcatcc agtgtggaga ttttggcata gcgcaggctg
     5041 acgttgtggg cgaaaaagac ttccgccata tcggcgagca ggaaggggcg gttgacggcg
     5101 gtgatttcga cggagtacca atcggggtag tcttcttcgg gggtaatggt gatgcttggc
     5161 gcgatcggca tatagcggct gcggcggctg atgcggcggc tatggctgga gatttcggca
     5221 acggtgtgtc cgtggataaa gttgttgagt tcggcttcga gcgcgctttg gatgtcgggg
     5281 tagtcttcgg gagcgtgctg cgaggggatt tgcacgatga aggtgtcgag gatgtagtcg
     5341 tgttcggtga taaaggcgcg ggcggcgagg atatcgaagc cgtggcggct gaaaatgcgg
     5401 cagaggcggg cgaacaggcg cgggccgttg ggcataaaaa ccatgacttg aaagctgtcg
     5461 cttttgggca ggatgcggct gcggacgatg ggggtttcaa agtcgtggac taggttggcg
     5521 gcgtgccaca ggatttcgcg ggactggtgg cgggcgaagt aggcggaacc gagcgcgttc
     5581 catagttttt tctgctgttt ttcggggacg gcggcgcggg tgagtaagtc ggcggcttcc
     5641 tgccggcggc ggccgaagag ggtgtgcggg ttgccgccgt tgcctgtaag gtagcgtccg
     5701 gcggcatgga agaggctttc cagcaggctg gcgcgccatg cgttccacag cttgggattg
     5761 gtgccgcgta tgtcggaaat ggtcagaaag tagagcgcgc tgaggcgttc gtgggtttgg
     5821 acgcgtttgc agaaggcatc gagtacgctg gggtcttgga tgtcttcttt ttgggcgacg
     5881 gtggacataa gaagatggtt ttcaaccagc caggcgagca ggtcgctttc ttcttcggtc
     5941 aggaagtggt cggcggcaaa ttggcgcgcg tctgcgatgc cttgtatggc atggtcgccg
     6001 ccgcgtcctt tggcgatgtc atggaaaaag gcggcaaggt agaggatgtc ttgtttttca
     6061 aaggactgca tcagtgcaga ggcgtagggc agctcgtggc tgtgcatatc caaggcaagg
     6121 cggcggacgt tgcggacgac ggtgaggatg tggtcgtcca cgggatagat gtggaacagg
     6181 tcgtgctgga gcaggccgac gattttttcc cacgcgggca ggtagcgtcc caatacgccg
     6241 tagaggttga ggaagcgcag ggtttgggtc agtccgttgc cgttgcggaa aaagccggcg
     6301 aagcggtggc gattttcaga attttggtag aagctgcggt tgattttgcg cgtcgcgccc
     6361 caccatgcgc gcagggtttg cggttcgagc gcggtaatat cgttgcgctg ctgcatgatt
     6421 tcgacgattt tgaaaatgtg ttcgggccgt ctgaaaaaaa tatcggtgtg ccgcgcggcg
     6481 atttggttgt tgacttggat gtagtcgtcg tcaatccgca gggtaacgcg caacggggtg
     6541 gaggaaacgc ggctttgcag cataggcgtg aggatgccgc ccagttgttt gacggtttta
     6601 atcgcgcggt aaaacacgcg catcagttct tcgctttggc ggcggaggtt caagccttca
     6661 taacccatgc tttcggcgac ttgcggctgc aaatcgaaca gcaggcggtc ttcggcgcga
     6721 ttggcgttta aatgcagatg gatgcggatg tgggcgaggc ggcggtagcc gtgcgaaagc
     6781 ataccggctt cggcacgcgt caaaatccgc tgtttgagca ggtcgggcag gttggtcgcc
     6841 aagccttgtg ctttcgctat ccaaagcagg gtgtggatgt cgcgcagacc gcccggacag
     6901 cttttgatgt ttggttccaa taccgcccct gagccttgcg atttggcgtg gcggtgttcc
     6961 atctccacca gttttgcctc gacaaacgcc ggcacattgc gctgcgcgtt catttttttc
     7021 gccagttcgt ccgccgtttg gcggttgcca aacaaaaacc tagcctctaa aaacgctgtg
     7081 tcccccgtaa tatcattgcg cacgctttca catagttcat caacgctgcc gctttttaca
     7141 gacggcatca gtttgcagtc ccacagggtt tggacaaacc gggcaatctg ttcctgaatg
     7201 ccgtctgaaa gcggggcagg ggagacaacc gccaaatcca cgtccgaaca gggatacagt
     7261 tcgccgcgcc cgaagccgcc taccgccatc aggcataacg cgctgttttg aaaatactct
     7321 gcccacaatg ccgccagtaa ggtttcgact gccgccgtgt attctctgaa aaataccgac
     7381 acgcggttgg ctttcaaata atgcgcttcg gcggcatcgc gctgctgttt gaaggtttcc
     7441 agtgctgaag acaggtttgc aggcattttt attctttcga ttggcgggaa aaagggaggc
     7501 ggatggttcg gcggtcaaat accgctttca gacggcattt gtcgggtatc ggcggaattg
     7561 gtcatcagct tcaaccgttc ctgcggcggc agcagcaact gatacgccgc cacgcccaaa
     7621 gccgccgcca ttttttcgat attcgacagg gcgatgttcc agcgtttgcg ctcgactgcc
     7681 gacacataag tcctgtccaa accgcattgc cgcgccaatt cttcttgcga ccaacccttg
     7741 ttcacgcgga aaagccgcat attgtatgcc aataccgccc gcaaatcctg ttcgtcaggc
     7801 agttcggcag gcagagtcaa tttgttgccc atcatttgct tccaaataaa tggttaaagt
     7861 taagcatttg ctgttacgga ttttacttca cataaaagcc aaaaaaaggg gtggcggcag
     7921 ccacacccaa acacacacac atcaagagga aagaggtaac atcaagacag attgggaaac
     7981 agcaaacaag caatctagat ttcatatcgg tattatcaaa caaaaaccgt tatcgagtat
     8041 tgattcaaat caattgagat ttgattcgca aaccggtaaa gaaaaacggc atacccgttt
     8101 atacggatat gccgtctgat gatgcagggg actgctgtat ttggcagtgg tttgtgtttt
     8161 agtcctcttg cgccgctgtt tgcaggtagt tcggcaaacc aattttgccg attagttcct
     8221 gctgggtttc gagccagtcg atgtgttctt cgttggtgtc tttttgtttt tccaacaaat
     8281 cacggctgac ataatcctgt tgcgcttctg ccacggaaat tgccgcaatc aatgcctcat
     8341 gtttttcttt ctctttttcc aaatcgcagg cgatgatttc ttcggtggac tcgccaatca
     8401 gaagcttgcc cagttcttgc aggttcggca gaccttcgag gaacagaatg cgctcgatca
     8461 aatcgtcggc agctttcatt tcaacgatgg actgtttgaa gaaatgttcg cccagttctt
     8521 caaagcccca gtttttcaaa atacgggcgt gaaggaaata ttggttgatg gttaccagca
     8581 gcaagcctaa gtttttgttt agctcgcgga taaccagacg gtcgcctttc atacggactc
     8641 cttttattcc gaatacggat tacagttggc tttggtagta gttgccttcg ccaattaact
     8701 cgatcaggcg aagctgctgt tccagccagt gggcgtggtc ttcttcggta tctttcagtt
     8761 gggcaaccat caggtcgcgc gtaacatagt cttgagcctc ttcgcacagt ttgatgcctt
     8821 ttttcaacgc atcacgtact tcatattcgg tttgcaggtc ggctttgagg caggaaacca
     8881 cgtccgtgcc gatattcagt tcggcgcgtg ccattttcgg cgtgccgccc agcatcagga
     8941 tgcggcggat gaagtcttcg gcgtgtgtgg tttcttcttc catctcgtga ttgagacgtt
     9001 caaaaagttt ggtgtagccc cattcggagt agaggcggga gtggataaag tattggtcgc
     9061 gtgccgccag ctcgccagac agcaattcgt tcatataatc aacaacagct tgattgcctt
     9121 gcataatatc tctctttccg taacttgatt ttcggtatgt ctgaacagca tctgtatcgc
     9181 tattcgggat gcgtgcattg tatgcaaaag tttttgatat gacaaatttt ctatttaaaa
     9241 tacaaacaat tatcaaaaga aatagggcgt attccccgat gccttccaaa tcagtatgct
     9301 gtttcttatc ggttttttgt gctgttaaaa ctaagaatcc atatcatcca taaagaacat
     9361 ttacacttgt taaccatagc gaaaagcaaa tactgtacgg ttttttatca aaatttataa
     9421 tgaatcgttc tcattaaccg acagattctt ttgagattat cggattttgg gaataaatta
     9481 tgccgtctga ggggctttca gacggcatag acaattaaag ctgattaaac aacgcggcag
     9541 acagcagcgt aggaaattat tttttacttc ggaacggtat tttgggttta atgatgcccg
     9601 caaccgccgt ggctttcgcg taaggcttcg gcagcctgtt cgtcggcgtg gtagctggaa
     9661 cgtaccatcg cgccgatggc ggcattgctg aagcccagtt cgtatgcttc tttttcaaag
     9721 attttgaact gctcgggcgt aacgtagcgc aggacgggca ggtgtccgtc tgaaggctgg
     9781 aggtactgtc cgatggtaat catttcgata ttgtgcgccc gcatatcgcg cataatttca
     9841 cgcacgtctt cgtctgtttc gcccaagccg accatgatgc cggatttggt cgggatgtgc
     9901 ggcatcattt ctttataacg ttttaataag tctaaagaat gttgataatt ggcaccggga
     9961 cgggcttttc tgtacaggct cggatgggtt tctaggttgt ggttcatcac gtcgggcggg
    10021 gtttcggcaa ggattttgag tgcgatgtcc aagcgtcctc ggaagtcggg gacgaggatt
    10081 tcgattttgg tgttcgggct ggtttcgcgg atggctttga tgcagtcggc gaaatgctgt
    10141 gcgccgccgt cgcgcaggtc gtcgcggtcg acggaggtga tgacgacgta acgcaggttc
    10201 atggctttga cggattcggc gaggtttttc ggttcgtcgg ggtcgagcat attcgggcgg
    10261 ccgtgtccca cgtcgcagaa cgggcagcgg cgggtgcaga tgtcgcccat aatcatgaag
    10321 gtcgccgtgc ctttgctgaa gcattcgccg atgttggggc aggaggcttc ctcgcaaacg
    10381 gtgtgcatct tttgttcgcg caaaatgtct ttgatttcaa agaatttgcg cgatgggagt
    10441 ttggcgcgta tccattcggg ctttttcagt ttttcctgaa gggggacgac tttgatgggg
    10501 atgcgcgcgg ttttgtccgc gcctctgagt ttgatgccgc gtttggggtc gtcggttttg
    10561 atttcactca ttgttgtctg ctttcggtgt gaattgtgtt tcaaggtgtg cggtaagttt
    10621 ggcggcgact tcgtccggcg tggggcaggg ttggacaaaa tccgcgattt gcgtcatttc
    10681 cataccggcg tagccgcagg ggttgatgtg ggtaaacggg cttaaatcca tattgacgtt
    10741 gagcgcaagc ccgtgataga cggagccgtt tttgatacgc agccccagtg aggcgatttt
    10801 gcgttctccg acataaacgc cggggcgttt ggggtctgcc gccgcttcga tgccgtattc
    10861 tgccaatgtg gcgatgatgc tgttttcaag cgcggacacg atgtttctaa cactggtttt
    10921 gcgccgtttg aaatcaatca tcgtataaac gaccaattgc ccgggcccgt gataggtaat
    10981 ctgcccgccc cggtcgattt ggacgacggg aatgtcgtcg cgaatcagca ggtgttcggg
    11041 ttttcccgcc agtccttgtg tgaacacggg cgggtgttcg acgacccaca gttcgtcttc
    11101 ggtgtcggca ttccgtccgg cattaaaggt tttcatcgct tcaaaagtcg gcagatattc
    11161 gaccaaacct ttgtgtatga ttttcatctc aaagtaccac tttgaccagt tcgtgcgaag
    11221 tcagcgcgcg gtagatgttg tccaattgtt cttggttttc aacctttacc tgtacggtgg
    11281 cgccggtata gttgcctttg ctgctcggac gcgtggtgat gtggtgcgcc tgcgtgtcgg
    11341 gggcgtggag gcggacggtg tccaataccg cctgctcgaa ctcgggatgc accgcgccca
    11401 ttactttcaa tgggaaggtg cagggaaatt cgatgaggga tgttttgttt ttttgttcgg
    11461 tcatgatgtg ctgccttgtc gtgtacggta tgccgtctga aggcgggttt gcctttcaga
    11521 cggcatcgga tgtgcgttat tttagcctaa accgcgataa caggctatcg ggaaggcgga
    11581 ggcttttttg acggcgcggc ggttctgcta tactggcgca caatattatt tttagaaggg
    11641 tggtttttat gtatcggagg aaagggcggg gcatcaagcc gtggatgggt gccggtgcgg
    11701 cgtttgccgc cttggtctgg ctggttttcg cgctcggcga tactttgact ccgtttgcgg
    11761 ttgcggcggt gctggcgtat gtattggacc ctttggtcga atggttgcag aaaaagggtt
    11821 tgaaccgtgc atccgcttcg atgtctgtga tggtgttttc cttgattttg ttgttggcat
    11881 tattgttgat tattgtccct atgctggtcg ggcagttcaa caatttggca tcgcgcctgc
    11941 cccaattaat cggttttatg cagaacacgc tgctgccgtg gttgaaaaat acaatcggcg
    12001 gatatgtgga aatcgatcag gcatctatta ttgcgtggct tcaggcgcat acgggcgagt
    12061 tgagcaacgc gcttaaggcg tggtttcccg ttttgatgag gcagggcggc aatattgtca
    12121 gcagtatcgg caacctgctg ctgcttccct tgctgcttta ctatttcctg ctggattggc
    12181 agcggtggtc gtgcggcatt gccaaactgg ttccgaggcg ttttgccggt gcttatacgc
    12241 gcattacagg caatttgaac gaggtattgg gcgaattttt gcgcgggcag cttctggtga
    12301 tgctgattat gggtttggtt tacggcttgg ggttggtgct ggtcgggctg gattcggggt
    12361 ttgccatcgg tatgcttgcc ggcattttgg tttttgttcc ctatttgggc gcgtttacag
    12421 gactgctgct ggcaaccgtc gccgccttgc tccagttcgg ttcgtggaac ggcatcctat
    12481 cggtttgggc ggtttttgcc gtaggacagt ttctcgaaag ttttttcatt acgccgaaaa
    12541 tcgtgggaga ccgtatcggg ctgtcgccgt tttgggttat cttttcgctg atggcgttcg
    12601 ggcagctgat gggctttgtc ggaatgttgg ccggattgcc tttggccgcc gtaaccttgg
    12661 tcttgcttcg cgagggcgtg cagaaatatt ttgccggcag tttttaccgg ggcaggtagg
    12721 cggttccgaa acatatttga agcggaatac aaccttgccc gggtttaaat aaaaatgccg
    12781 tctgaacccc gaaaacagag cttcagacgg cattttcatc acggcttatt tggcggtttt
    12841 gctgctgtcg ataattttca taccggcaga aatcaggctg ccgatgtcgg caacattggc
    12901 gggcataatc agcgtattgc tttctttggc aagattgttg aacgcggcga cgtattgttc
    12961 cgcaatcttc agattgaccg catccgcgcc gccttgggtt tgaagggcgg cggcaatttg
    13021 acggatggct tcggcattgg cttcggcaac aaggcgcaag gattccgctt cacctttggc
    13081 gcggttgatg cgggcgattt tctcggcatt tgacgcattg accgcagcct gagcctcgcc
    13141 ttcggattgt tggatttcgg cttcgcgctg accactggca aggttgattt gttcgatttt
    13201 acgaccttcg gattcggcga tacgggcgcg tttttcgcgt tcggcagtaa tttgcgcctg
    13261 cattgagcga aggatttctt gcggcggaac caagtcttta atctcataac gcaaaacctt
    13321 cacaccccaa gccccggccg cctcgtccaa agccgcaaca acagtactgt tgatttcgtc
    13381 gcgttcttca aacgttttgt ccaactccat acgcccgata acggaacgca gcgtcgtttg
    13441 ggcaagctgg gtaattgcca tgatgtagtt gctcgaaccg tatgaggcga gtttggggtc
    13501 ggttacttgg aaatagatga tgccgtcaac agtcagctgc gtattgtcgc gcgtgatgca
    13561 gacctggctg ggtacgtcta aagggatttc tttcagcgaa tggcggtagg caacgcggtc
    13621 gataaaggga atcaaaatat tcaaaccggc cgtcagggcg cgatggaaac gcccgagcct
    13681 ttcgacaacg tggacttcct gttgtgggat gacaacaaag gatttgaaac cgaaaacggc
    13741 aacggctgcc aacaagataa tgaaaaattc cataattcct ccgagtgtta aggttttgcg
    13801 atgataagaa ggttgccttc cttgcggacg attagggcgc gcgttcctgg ttcaagctct
    13861 tcttgccccg tattttgcgc ctgccagtgc gtaccgcgat aaaaaacttc gtaacggttg
    13921 ccgcctgtgt gtcggaggat ttcgacatat tgtccggcat ccaaatcctg atatgaatcc
    13981 gtttcaactt ttctaacggc ggttttggcg tgtacgaacc aaatacccag cgcggaaagc
    14041 agagcggcgg tcaagacggc ggcaggcgtg ctgccggtca gcccgtaagc aatgcccgaa
    14101 cccgccaaag ccgcgctgac aaccaaaaga taaaccgttc ccgtcaataa ttcgatgatt
    14161 aagacggcaa cagcggcaac aaaccataca gtcatacatt tccccacaaa gcgcgtcgtt
    14221 tgacaaaata acgcaatatc agcagtatag ccgaatttga aaggataggg cagatatgga
    14281 cacttggcac gatgcactcg gcggcgaaaa acagcagccg tattttcagg aaattttaaa
    14341 tgcagtcagg caggaacgtt tgtcgggaca aatcatctat ccgccggcgg cggatgtgtt
    14401 caacgcattc cgcctgacag cgttcgaccg ggtcaaagcc gtcattctcg gacaagatcc
    14461 gtatcacggg gcagggcagg cgcacggttt ggcattttcc gtccggcagg gtatccgcat
    14521 accgccgtct ttactcaata tctacaaaga attggaaacc gacatcgaag gcttttccat
    14581 acccgcgcac ggctgcctga cagcgtgggc ggagcagggc gtattacttc tgaacacggt
    14641 tttgacggtg cgtgcaggac aggcgcattc gcacgccctt ttaggctggg aacgctttac
    14701 cgataccgtc atcaggcagc ttgcgacaca ccgcaagcat cttgtcttta tgctgtgggg
    14761 tgggtatgca caacaaaaaa ggaaactgat agacagtcaa aaccatttga tattgaccgc
    14821 accgcatccg tctcctctgt cggcatatcg cggttttttc ggctgccgcc atttttcaca
    14881 ggcaaacagc tatttgagcc ggcacggtat cgatccgata aactggaagc tgtgaatgcc
    14941 gatatagccg ttgccgccgg cgtgttaaaa tcgcgtttga tttgtaattt ccatttatta
    15001 ggcaaaacct tatggcaatc aaaaaaaaat cagctttatg catcactttg ggcgggctgc
    15061 gacgagttgc gcggcggtat ggatgcgagc ctttataaag aactatgtgc ttacgctatt
    15121 gtttttaaaa tatgtttctg ataagcataa gtacggcggc ggcatgattg agctgcacgc
    15181 cggtacgact tttgacgaca tcgtcaaact caaaaacacc gccgacatcg gcgaccgcct
    15241 gaataagatt atcgcccaaa ttgccgaagc caacgactta aaaggcgtga tcgacgttac
    15301 cgacttcaac gacgaagaca aactgggtaa aggtaaggag atgatcgacc gtttgagcag
    15361 gcttgtcggc atttttaaaa agctcaacct ttcttccaac caagccgaag acgacgattt
    15421 gttaggtgat gcctacgaat acctgatgcg ccattttgcg accgagtcag gcaaatccaa
    15481 agggcagttt tacacgcctg ccgaagtctc ccgcattatg gcgaagatta tcggaatcag
    15541 cgcagattgc cgtccagcac cagcgttgat gacccgacct gcggctcggg ttcgctgttg
    15601 ctcaaagccg ccgcccaagc cggcagccaa atcagccttt acggtcagga aaaagatgtg
    15661 gcaaccgcgt cccttgcccg tatgaatatg attttgcaca acaacgaaac cgccgaaatc
    15721 aacaccggga acaccttgtc cgattcgtct ttccgtgatg aaaacgacgg gcttaagacc
    15781 ttcgattttg ccgttgccaa tccgccttat cccgcccgaa aaaaacggcg attacgcctt
    15841 tttgctgcat ctgctcaaaa gcctgaaacc aagcggcaaa ggtgcgatta ttcttccgca
    15901 cggtgtgctg tttcgcggca atgccgaagc gcgtattcgc acggaattgc ttaaccttga
    15961 ccttattaaa ggcattatcg ggctgcctgc caacctgttt tacggcacgg gcattcctgc
    16021 ctgcatcatc gtcatcgaca aagaacacgc ccaaaccgcc caatttgccg aagagggaac
    16081 aaaccaagtt atcagtggcg gcagcgtgtt tatgattgac gcatcgcgcg gcttcattaa
    16141 agacggcaac aaaaaccgtc tgcgtgagca agacattcac aaaatcatcg acacttccac
    16201 aaacctcgtt acagccgtat ggtgcattta agcgaaatcg cagcacaaga ttacaacctt
    16261 aatctgcctc gctatatcga cagcggagaa gttgaagacc tgcaaaatct cgccgcctat
    16321 ctttatggcg gcatacctgc gcacgatatg gacgcattgg aagcctattg gcaagtttta
    16381 ggccgtatga aaaacgagtt gtttgccgaa cacgatggcc gctttaccac tatacaacgg
    16441 aatcgattgc aaatctttcc cactctcaac agcttaaaat cctgcgggat tggtgtggaa
    16501 tttagggcta atctagtaca gccccaaatt taatccacta taaaatcgaa agcagccaaa
    16561 tcaaagccca tatattggcg caccccgatt acgccgcctt caaagccgga cacctggcaa
    16621 agtttgccgc gtggcacact caaaacgacc ttgccgccat ccaaccgggc aggcttatcc
    16681 ggaaatggag cgaaagcctg ctggacgcgt tcaaacccgg cagcctgatt gaagaatacg
    16741 atttctacca aatcctgacg gactactggg cggaaaccct gcaagacgat gtttatctca
    16801 tcgcccaaga tggctggaag gcggttaaaa acctggccga aatcaccaaa gaaagcgatg
    16861 aagacgcgaa cctgaccgtc gtctttgagg aaaccgaaac cggcaaaaaa ggcaaagcca
    16921 aaaccaagcg catcagcaaa aaataccgca gcgaagtcat cgcccccgag ctggttgccc
    16981 gccgctactt ttcagacggc atcgccaagc tggaagaaaa acaaagcgag ctggaacgcc
    17041 taagccaaga attggaaaac cacatagaag aacacggcgg cgaagagggt gcgctgaacg
    17101 acgtattgga tgcaaaaggc aaactttccg ccaaacttct gaaaaccgca ttggaagaaa
    17161 gcggcataga agaaggcgaa cgggctgttt tacaaaccac ccaaacactg atgacgcagg
    17221 aaaaagccgc gaaagacgca gtcaaaaccc aaatcgaagc cctgaacctt gccgtattca
    17281 aacaattcgg ccgactttcc gaagccgaaa tcaagcagct tgccgttcaa gacaaatggc
    17341 ttgccgattt acaaagccga atcgaaaatc gcttggaaaa cagtattcag cagcttatca
    17401 gccgcttgaa cacgctggaa gaccgctacc gcagcccgat ggccgagctt gcccgagaag
    17461 tggaaaagtg gcaaagcaaa gtcaatgccc accttgaaaa tatgggtttt ggaggctgaa
    17521 atggcggcac agacaggcta taaggcgagc gggttttgag acctttgcaa aattccccaa
    17581 aatcccctaa attcccacca agacatttag gggatcgcgg ttcgggtgtc cgcaccgctt
    17641 aatacgtcgt cgtccacgaa ctgacccatt tgctcgaacg ccatcgcaac gcccgtttta
    17701 tggcgcatat ggacaacttt ctcccaaact ggcaaagcat caaacaacag cttaatgcct
    17761 tggagttatt tgcacaaata tataatttaa cataatatat attatgcgaa ctagaataaa
    17821 tatgtaaatt tcatatttca aacctgttcg attgcggatg tttttctctt tataaatcaa
    17881 ttaaatataa ggaaatatta tgtccgcacc cgtaatattg tctgcagccg ctacaaaagg
    17941 tggcgtaggt aaaacaacac tcattgctaa tgtctctgct gttctcgctg atatcggact
    18001 gcgtgtctta atgattgact gcgatgttca gccatcattg tcaaaatact atccaataag
    18061 ccaccgcgct ccaaatggta ttgttgaatt acttctcggt gaaaataccg aagaaatcat
    18121 ccgctcgaca atttccaata ccgtttttcc gaatttagac attgttattt ccaataacat
    18181 ttctgctttt gtcgagactg aagtaacctc tcgtcccgac cgtgccttct tgctcagaag
    18241 caaactgcat tcccaataca tacagaataa ctacgatgtg gttttaatcg atactcaggg
    18301 tgcagttggt cctctcttag ataccgcagc atttgctgct acatgcctgc tttgcccaat
    18361 tatgcccgaa gttttgagtg cccgtgagtt cctcactggt acgcaagaac ttctaaagcg
    18421 tctgcaaaaa ggtgaagcaa tgcgtatccc gatgcctcaa ttacgagcca tcatctacgc
    18481 acaagatcgt accaatgatg cgaaattcat cgcatcttct atcaaagatt tctttagcaa
    18541 tactctagat gacagacgta aattgttaag cgtagcagtt cctgctgcca aagcatataa
    18601 ggaagctaca actttaagag tcccggttca ttgtcatgag cgtaaacagc ttggaaagat
    18661 gctgcctgct tatgaaatca tgcaccgtat tgtttatgag attttccctg gtatcgaaca
    18721 gaagaacctg cgtggaagct gtttcaatga catggccagc ctgctgcaag aggaggtgca
    18781 atcatgaatt tggaccaaaa taaagcaacc ttgagcctgc acatcggtac ccctgcacag
    18841 acaaatatga gttttggcca agaggatact ccttctcggg gcatgttaat gtcgttgcca
    18901 atcagcgaaa tcgatttctt tgacaaaaac cctcgccgtt tacatgatgt ttccagctat
    18961 gcggccatca aagagtccat ccgttcttca ggtattcaac aacctgtaca cgttacccag
    19021 agacctggtg aaagtaaata tgtactggca cagggtggta atactcgtct caaaatcttg
    19081 tgcgaactct tggaagaaac tggtgataac cgcttccgta tgatgccttg tatttacatc
    19141 cactactctg acgaaactac attgcaggtt gcgcatcttg ttgaaaatga actccgtgct
    19201 gaaatgtgtt tttgggataa ggcctgtaaa tatgccgaaa tccgcgatat tttccaagat
    19261 gaatctgact cagggaaact ttctttgcgc gaactggaag tgatgttttc tgataaaggc
    19321 cttacggttt ctcataagac tttaggatta ttcttttttg cctccgataa tttacaagag
    19381 ctgcaagaat acgcattcaa actttcccat gcaaaagtag aagaaattaa aaaacttcac
    19441 tctaccctat tctcaatttc taaatcaatt cagttggacg attctttcca atatttgtgg
    19501 tcagatgtat tggagcagtg gcataaccaa tattctgcaa cagagttaga cgtgcatgag
    19561 ctttatcaat tcgtgctcga cagatttaaa tttgaatatg gctctgaagc cttttctgaa
    19621 acagaaaatt cttctgataa agacaaaatt gaaacagccg tcaaacaaga tgtcaatttg
    19681 catcaaaaag aggtatcgga aaacaacact gagtcagagg attcggatat ggaatttgtg
    19741 ccagccaaag taaatggtac taaagctgat gcagaggatg agccgaaaga agccgaactg
    19801 attactttgg agcaaaccag caaaaaactg ttttctgctg ttcgtaaact gttagccaaa
    19861 gtgagtctgt ccgattgctt taagattcat aaggactttc catacggctg gtatgtcgaa
    19921 tatccagctt ttgagcatat ttcagcatca agtgccggaa atgccatcta tgctattgat
    19981 gttttgcatg aagaagcagg aaatgttttc acattcttat ggaagcttag tggaatcgac
    20041 acctttatgt ttgatgttgg tgatagttcc aatcccctgc tgacacttaa aagtgattca
    20101 aagcttcgta ttgcctacga ggaccccgat aagtacgacg aatatacgaa ctgcggaatt
    20161 ggtaataaag agaacattat tgcctcttta ctgaattggc aaacaaatac cagccatgaa
    20221 tttcacgcca atatcgtaga aatattatct ctgagcacac agtacaacaa cttgagaaaa
    20281 acgggagcta agagtgagga gtaagccttt aattagtaat cccatttata gactgtttaa
    20341 tacggatcca gtttatacca atcttgcact tacaaggttg gcatccgcac caaatacgcc
    20401 gcgtgaatct tcttataacg ctttggttgc tcgtgaacat gaggatgttt tgaacacgct
    20461 caaacgaatg aacccgaagc ggattgagaa attagcatcc atgtgtcctg atgttgtgac
    20521 ctactccatt gatctgaagg ttttaaaaag caaaattaca gaaatggaac attacgaaca
    20581 aaggtttatg gaacagatgc agcgtcttaa atggatgtta gaacataagg ccagcaatct
    20641 aatgattatg aatctatgtt cattaatttc tacggcggaa attaaaaaat tgcgtataga
    20701 aatgaatatt cctgtcgtta aagggcgcat tgtgatgcca tctattgatg ttcgcgtcag
    20761 ggtattgaag gcatggagta agtcggaaaa agagacagat ttgtttatta gataccagct
    20821 attgattgat gaattccccg attatagtct tgcccaatta cattcaataa ttaacgatgt
    20881 aaagttcttt ggagtatgag tatgtatatg gatttctcta cttcctatga ttatgagact
    20941 ggttttgtac catctaagaa catggcaacc gataacttta ttcgtagctt ggttgccttg
    21001 tgggaggaac cggccacata tgtgcgtcag tttgctcagg cagtcggttc tgtttcagag
    21061 ggctattttt tgagttggtt tgtcgagaaa tgtctgcctg gcgaatgggt aagcctgaat
    21121 aatgatctgt tgtttaatga agcaggtatt accgcaaagc agtggcggaa tattcgcaat
    21181 agccttctgc agaaaggtat tttgcttaat cggagaaccg tagcaccaac cgaatcacaa
    21241 tttacattga atgatgctct ttttgaggat ttggtaagta aacatggtaa atcagatgta
    21301 cttgacatgg tttcgccccc tatttcgatt aataaacttc acctgaagac attagctgat
    21361 agaggtcttg cttttgtatc cattttgtat ttgtcgtttc ttcaaagtga agctgaatat
    21421 caagatttga gtaagcgtgg tgactggtct ccttggactc atgcagctga aaagcacgtt
    21481 ttttattcaa caatgttgtc aagacggcaa caggaaagat ctattgccga attaacctct
    21541 tttggtttga ttgagagctg tgttgatggt atgccagcag cgcgacgttg tagatacagc
    21601 ttgaaaatgc ttgctgatct tacttctgaa tatttgaacg gatgtgtgtg atggttaccg
    21661 ccaccaaatt acccccttcc aaagtattta actctgaact gacacaatgc tggcaagcgt
    21721 tgcaaggtag tcgaagagtt gccatctatc gatgtttaat tgatattaca ggctctctga
    21781 agactgctgc aatgctttct cagttaatct attggacaag agtgagcaaa gaggttgccg
    21841 aacgggatgg ttggattttc aagtctattg ctcagatgga agctgaaact ggtttgacag
    21901 tgagagagca gcgcacttgt aaaaacaagc ttcttgagac aaaccttatt caaacatgcc
    21961 gtaaaggtgt gggagctgct ttagcaatca aagtgaactt ggatgccata gctgagttga
    22021 tcgctaaatc gagcggtact gacgatcaac ttgcacttac gctggctgat ttacagaaca
    22081 caagttccct gtattttaga aaacactttt ctaaaaggat tgcttatcac cgagacctgg
    22141 tttctattac aggctgtatc aactcggctg tattattgtc gtgtgtgtta aatgatgcgg
    22201 ttggtttggt ttctcaaaat ccacatttac aacgtcatgc ctttgctact ttaactgcta
    22261 ctgattggga ggaaagaacc tctctctctt ataaatctca attgacagct cgcaataggc
    22321 tgaagaattt aaatctcatt tatgagagaa atttcttggc cagccgccgt atttttaccc
    22381 tggtaaatgg tcacgatatt gttatatcca ttaaaaaact tcttgctggc aaacaggacg
    22441 acggcttcag tccatataac tccaaaaaac gcgaaattct cagtttggcg gaaagggcca
    22501 aatgtgattg gcggaaaggg ccaaatggat tggataaagg gtttcagagc gattccgagg
    22561 gtgatgcccc cctattccca agtatgacat ccggaagtgt cgaaaggaaa tattcggatg
    22621 tgtcgaattg gaattttaag aaaggtcata gtgggaaatt cacaagtgtc gattgggaaa
    22681 gttcagattt gtcgaatcgg cagattattt attatataaa ttacaaaggg gtttataact
    22741 acaactacat gactgttgaa cccaacaaat cacgttcttc cgaacaagat catgtggtag
    22801 tggttgatga tgttgatttg atttatccaa aactttttaa tgtgcaaatg cgtcaacagg
    22861 cggatttgat ttttacaaaa catctctcaa aaagttcctg ggctttgagg caggaaatct
    22921 tggatgagat ccagggacag aaaaaagaag ttactagtcc ccttggtctt ttgattaacc
    22981 tatgccgttg cgcttcgcaa ggtaacttta ctgctcttgt cgccccacag gttagattcc
    23041 agcgtgagca agatcagaaa ttacaggctt atcttgaaaa gcagaataac caagaacagg
    23101 actcattaga taccatggat gtcgagcttg cccgcaataa tttaagaaag ttctactcca
    23161 aaaaatagaa tttaatttca aaaaatattt ttgatgaatt tttgaaagac gaataaatct
    23221 attacttcga agcggatgta acggggcgtt acagtcatcg gcaaaggaaa gatatgatgg
    23281 tgtacatgac taattagcct ctgatatgca acaacagtgc tttatgaagc tcttattaat
    23341 aacttataag gatttaaaaa tggaatttgc aaaacaaaga tatatcatca aacctggtgc
    23401 gatccatgtt gcacagcaat ttgatctcaa agtcacaaca aaacagtcac ctttcagtga
    23461 cggttacgat atggctcgtg ctgctttggc tttggggggt aaggtcaatc aaaagttgac
    23521 tgaggatgac gaacagttcc aacagtggga agagtttaaa gcacatgaat tgctgctcaa
    23581 acaacatcta caaaaggaga caggtcgaaa gcatcacttg gtttgggggg agtatgaaat
    23641 tctttcttct gagaacgacc ggtttaaaaa tattggcagc ctgacctctt ccggtgatga
    23701 tggttttttt atgcatacca agcaaggtat ccgtatgtgg gagggcaata acaacaagaa
    23761 gaacggccca cgttggccgg gaatcagata tggtctgaca ctcagtaacg agttgtttgg
    23821 tatggctatg tccgataatc cttatgctca aagtcggtta ttgcagattg aaaaacagat
    23881 gtccgaaatt gaggaatttt ttgagactgt aaaaaaacag gttcatgctc agattgatag
    23941 tttggccgct gcaggtatga agataacctt gattcagaac aataaccctg ttcatatcag
    24001 attgaatgta ttgcgggctt atggtttcaa actggttaaa ttgttgcggg attacgatag
    24061 ttttgtttgt gccgttaaga cacttaatat caaaggcatg atggctaata aacaatgtaa
    24121 tgccacgttg tataatggcg gtcggatgat gagaaagcta ctgaatgatt tatatattgc
    24181 ggtaatggag accagggcta tcaagagcat tcgacgcgac agtctgcttg acccggagca
    24241 gttgtcgaag ttgaaatctg ctgttgagca agagattctg cctagaattc cattgtctgt
    24301 ttgggtgtat gaatcacaac cctctttggt ttatatccag agaaaaatga atcaggagga
    24361 tctcaataag ttggttgata ttgttcgtga caatggatta tcgggctagt tgtgaaattg
    24421 gaacgtgggg tcacaatcga gttataagat ggtattgatt ggcccttgtt tgtatggatt
    24481 tttttatgat gtgatatgaa tactatgaat actcaaatat caggtatgaa aaaatactta
    24541 aaattttggg aatacaaaag gcttttaagg gtaaggggga ttgaggattt agaaaaagaa
    24601 attaaattga aattggttaa tttcgagcta ttaattgatg aagctcagtc gtttcatgaa
    24661 gcatgtcagg accttaatct catatatcct atagtaagag aacatctgaa attgagtaat
    24721 aaaagtttgg ctggtttgga tttaaaaaaa tactatattc ccaatatgat ttttttcaaa
    24781 aatgttgttt cttccagtgg ccgtatgtcg agaaagaagt tttttaaatg gacag
//
LOCUS       DAXHKX010000010        24827 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHKX010000010 DAXHKX010000000
VERSION     DAXHKX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.010"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(106..354)
                     /locus_tag="ACFMWZ_000384"
     CDS             complement(106..354)
                     /locus_tag="ACFMWZ_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934400.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
     gene            complement(400..822)
                     /locus_tag="ACFMWZ_000385"
     CDS             complement(400..822)
                     /locus_tag="ACFMWZ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="wall-associated protein precursor"
                     /protein_id="HFC5934401.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPIQLA
                     ELSQKEMKETEGAALWFAPVLAGGGRFALTGFTRHGLNQAISRGGVGVSNKAIMNTMR
                     NPTKITSQSGGRTRFTGADGGVVLNSQGKVITTWGKPR"
     gene            complement(865..1734)
                     /locus_tag="ACFMWZ_000386"
     CDS             complement(865..1734)
                     /locus_tag="ACFMWZ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC5934402.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            1898..2821
                     /locus_tag="ACFMWZ_000387"
     CDS             1898..2821
                     /locus_tag="ACFMWZ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC5934403.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(2899..3540)
                     /locus_tag="ACFMWZ_000388"
     CDS             complement(2899..3540)
                     /locus_tag="ACFMWZ_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC5934404.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(3595..4386)
                     /locus_tag="ACFMWZ_000389"
     CDS             complement(3595..4386)
                     /locus_tag="ACFMWZ_000389"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC5934405.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLNVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            4552..4627
                     /locus_tag="ACFMWZ_000390"
     tRNA            4552..4627
                     /locus_tag="ACFMWZ_000390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Thr,seq:tgt)
     gene            4662..4737
                     /locus_tag="ACFMWZ_000391"
     tRNA            4662..4737
                     /locus_tag="ACFMWZ_000391"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4695..4697,aa:Thr,seq:tgt)
     gene            4879..5670
                     /gene="panB"
                     /locus_tag="ACFMWZ_000392"
     CDS             4879..5670
                     /gene="panB"
                     /locus_tag="ACFMWZ_000392"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC5934406.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVACPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            5660..5836
                     /locus_tag="ACFMWZ_000393"
     CDS             5660..5836
                     /locus_tag="ACFMWZ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934407.1"
                     /translation="MQTEQGLLLMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            5793..6629
                     /gene="panC"
                     /locus_tag="ACFMWZ_000394"
     CDS             5793..6629
                     /gene="panC"
                     /locus_tag="ACFMWZ_000394"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC5934408.1"
                     /translation="MQIIHTIQELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            6794..8632
                     /locus_tag="ACFMWZ_000395"
     CDS             6794..8632
                     /locus_tag="ACFMWZ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC5934409.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVVKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALTAAMMYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            8772..9353
                     /locus_tag="ACFMWZ_000396"
     CDS             8772..9353
                     /locus_tag="ACFMWZ_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC5934410.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            9363..10208
                     /gene="ispE"
                     /locus_tag="ACFMWZ_000397"
     CDS             9363..10208
                     /gene="ispE"
                     /locus_tag="ACFMWZ_000397"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC5934411.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            10216..10291
                     /locus_tag="ACFMWZ_000398"
     tRNA            10216..10291
                     /locus_tag="ACFMWZ_000398"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10249..10251,aa:Gln,seq:ttg)
     gene            10304..10379
                     /locus_tag="ACFMWZ_000399"
     tRNA            10304..10379
                     /locus_tag="ACFMWZ_000399"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10337..10339,aa:Gln,seq:ttg)
     gene            10388..10463
                     /locus_tag="ACFMWZ_000400"
     tRNA            10388..10463
                     /locus_tag="ACFMWZ_000400"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10421..10423,aa:Gln,seq:ttg)
     gene            10630..11613
                     /locus_tag="ACFMWZ_000401"
     CDS             10630..11613
                     /locus_tag="ACFMWZ_000401"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC5934412.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            11680..12258
                     /locus_tag="ACFMWZ_000402"
     CDS             11680..12258
                     /locus_tag="ACFMWZ_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC5934413.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKLKRNENLAVATVTGKKR"
     gene            12678..14561
                     /locus_tag="ACFMWZ_000403"
     CDS             12678..14561
                     /locus_tag="ACFMWZ_000403"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC5934414.1"
                     /translation="MIHDLEDNFKQNNILKLKQLFPEVFCEDQIDFEKLKLVLGAENL
                     AGAGERYQLDWAGKTASYLNLQSPTSRTLIPCKEESADFDGTQNVFIEAENLEALKIL
                     QKSYAGSVKMIYIDPPYNTGSDSFIYPDKFSESREEYARRVGDTDDAGYLKRDGVFQG
                     AWRKNGKDSGHYHSNWLSMMLPRLHLAKTLLREDGVIFISIDDNEQAQLKLLCDEVFG
                     AENFVTNIIWQKKYRPQNDAANFSEEHDFILCYAKKKKRKKSDYGWERNLLQRTAEQT
                     SDYKNPDNDPRGAWSSSGLMIEGTAKNCIYPIVGPTGIEFYPSAGKRWSISQDNFEKL
                     NSENRIWYGINGNAAPRVKRYLSEVQDGIVPSTIFLFSDVGHTGEGKKDFLKIFSEEL
                     FDYPKPIRLITKLLTIGSNSDDLILDFFSGSGTTAHAVMQLNAEDGGSRRFICVQLPE
                     ETDEKSEARKAGFNTIAEIAKERIRRAGRQISDGLQDGSEIDTGFKVFKLAESGFKQW
                     RQPEQSDTEALQGELSLNIDSVLSEMPSENLLYELMLRLGLKLTCKISLSDGMYFVED
                     EDTGGLYAFLLERVDQGLIDAVLAKHPVKVAVLDRLFDGDDALKSNTVLQMKDAGVVF
                     ECV"
     gene            14572..17223
                     /locus_tag="ACFMWZ_000404"
     CDS             14572..17223
                     /locus_tag="ACFMWZ_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC5934415.1"
                     /translation="MELKFEELPYQLDAVNAVTNLFAGQPNHTRTFDLTSQGTGRFVG
                     NGLDLDWETLGRNLNALQKQNGQPETEIGAHGLNFSLEMETGTGKTYVYLRTIYELNK
                     LYGWKKFVIVVPSVPIREGVLKSLEITKAHFDGVFGRPVMRYAEYSSSRLSDLRAFAV
                     NDHIEILVINIQSFEKDGNVINKVNESGEAPIKFIQETSPIVIIDEPQNMETEGRLNA
                     LESLHPLFTLRYSATHKNPYHKVYSLNPVQAYNLKLVKQIEVLPVLAENDVNGAFAEL
                     LEVKQAAKGRLKADLNIHYQDKKEIKKKKVSVKSGDDLFEKSGGNEAYRHGFIIDGMD
                     FENQELAFSSGKTITRGGDGDVVRDEIMKNQIRETVREHLAKEKRLNPLGIKVLSLFF
                     IDRVENYRAADGTAGKFALWFEEIYRELVGSTNTAGVHDGYFSQDKKGRLKNTDGTTQ
                     ADNDTYRLIMRDKETLLSFDSPLRFIFSHSALKEGWDNPNVFQICTLNETRSPLKKRQ
                     EIGRGLRIAVNQEGRRVRDEKVNILTVVPNESYELFAANLQKEYEDECGIKFAVENIK
                     NGNKKKKQLFRKDFPLDPEFQAIWERLKYKTRYRVRFDTEELIKQASEAVANLPEIQK
                     PRIRVRKAQLKQSQTYGIETVEIASRSREMDVQWDIPDILGEIQKKTGLTRRTVYEII
                     TQSGRLKDVAGNPQRFIDLAAEAVNRSLYALMAAGIEYEQTGEVYDQKLLEDMEREGI
                     EFYENSFTFEVGKPQKTICENYIPLDSGTEQAFARNCEDYDAENEGGVAFYFKLPGWF
                     KIPTPLGNYNPDWAVVKQVQDKVYFVAETKNTGKSIQAGVDAGKLRENERLKIECGER
                     HFEALGGLAYCVVEKVGDLKLEKEEQA"
     gene            complement(17387..18556)
                     /locus_tag="ACFMWZ_000405"
     CDS             complement(17387..18556)
                     /locus_tag="ACFMWZ_000405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC5934416.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTITEKEIVALEDVEKKNWWKKLWARLTGQ"
     gene            18704..20230
                     /gene="ilvA"
                     /locus_tag="ACFMWZ_000406"
     CDS             18704..20230
                     /gene="ilvA"
                     /locus_tag="ACFMWZ_000406"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC5934417.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(20287..21360)
                     /locus_tag="ACFMWZ_000407"
     CDS             complement(20287..21360)
                     /locus_tag="ACFMWZ_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC5934418.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGIWHYNGFAWKLDAHYKWQEQTAIGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(21357..22217)
                     /gene="cysW"
                     /locus_tag="ACFMWZ_000408"
     CDS             complement(21357..22217)
                     /gene="cysW"
                     /locus_tag="ACFMWZ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC5934419.1"
                     /translation="MKPYSANPNLTEPRRLRVLLITAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDSEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGRLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAI"
     gene            complement(22472..23308)
                     /gene="cysT"
                     /locus_tag="ACFMWZ_000409"
     CDS             complement(22472..23308)
                     /gene="cysT"
                     /locus_tag="ACFMWZ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC5934420.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAVEIGW
                     SGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            23447..23554
                     /locus_tag="ACFMWZ_000410"
                     /pseudo
     CDS             23447..23554
                     /locus_tag="ACFMWZ_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            23599..23931
                     /locus_tag="ACFMWZ_000411"
     CDS             23599..23931
                     /locus_tag="ACFMWZ_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934421.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVHRDAQFVQTANGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            24263..24772
                     /locus_tag="ACFMWZ_000412"
     CDS             24263..24772
                     /locus_tag="ACFMWZ_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC5934422.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         6181 a         5717 c         6847 g         6082 t
ORIGIN      
        1 actagagttt aaacaacttt atttttgatt ttatcccaag tataaacctg acaatcgtat
       61 ttaaaagccg gatatacgcc tagcttgttc actggtatgt atgatttaga ctaaaaagaa
      121 atcaatcatt tgctcaattt taagcgtctc cccgacaggt ttcccggtct caggatcaaa
      181 ctcaagaaaa cctgataaaa aatcattaat aatcaataat ttttcatcac tggaaacagt
      241 attttttata attttttccc ttagtacgga taaagcaaaa ttttctaaag aactatattg
      301 ccccccatca atttttttag aaattaaagc aactatttca tttttaatat tcataaatta
      361 cccttaagtt ataaaagggg caaagccccc tttttttgat taacgaggtt tgccccatgt
      421 agtaattact ttaccttgac tattcaaaac aacaccacca tcagctcctg taaaacgggt
      481 tcttcctcct gattggcttg taatttttgt agggttacgc atagtgttca taatcgcttt
      541 atttgaaact cccacaccac cacgagaaat agcctgattt aaaccatgcc gagtaaatcc
      601 tgtcagtgca aatctgccac cacctgccaa cacgggagca aaccataatg cagccccctc
      661 agtttccttc atctcctttt gagaaagctc cgccaactgt atcggcgcat ctgcctgcgt
      721 gtggaacact tggttttcaa atgtctgatt gtccaagccg tttgccattg cgggggcaat
      781 catagacagc atcattacgg ctgcggtgat ttgttttttc ataataactc ctttggatta
      841 caaggttgga aaatcaaaac ctgcttaaaa tgtatgctgt acgccaaatt tcagttcgga
      901 actgctttgc cctgaaacgt tgaaacgtgc ggatgcgttt aaagccgtgg ttttggtgaa
      961 accgaaacct gtgccgaaat gagcgtaggt ggatgtgttt cttgcggttt cttttttgcc
     1021 gtctatgcgg tcgggctgtg tgcccagcca ttggatgcct ccggtcaggc tgattctgtc
     1081 gttggcggca aatgagatgt tggggttgag cagcaggtag ttgcccgatt tgtagcggat
     1141 gccgtctgaa agggttttgc tgccgttgat gcggtaggcg gcggtgaggg aaaggacaat
     1201 cggatctatg gctttgtagg tggtggcgcc gatgagccag gattttcccg acgaggcttt
     1261 gttgcgcgat ttttcgtaaa ccgtgctttc aagaaagctg attagggcgg ggtttttgtc
     1321 gtctttaagg aaagtgtggc tgatgccgag ggatacgtcg gacatccgtt tgttgcgggt
     1381 tttgccgttg ccgtcgagtt tgcgttcttc gtgccacaga tagctgccgc tgccgtaaat
     1441 gtcggtattc ccggtcagtc cgtagcgcaa accgagcgtg ccaacgagca tatcggtatt
     1501 gctgccgttt tcttggattt cggtcggaat ggggataaac gaggttgcgc cggtttggat
     1561 gtaaaccggt gcggcaagtt cggcgcggtt gttttcgctg ttcaggtagg taagggaagt
     1621 ttccagtttc catttgccct tgtcggtcat tatgtcttca atcgtcaagg gcaggtcggc
     1681 ataagcggat aaaggcagga tggcgggcaa ggcgggcaaa aagatgcgct tcatatttct
     1741 cctattgata ttaattctta tttactgata ataatgaata tccgataatc ttgtctaaat
     1801 aaatttttaa atcaaaaaaa acgatatatt tgtttttgtc aattatgttt tgatatgccg
     1861 tctgaaaagt ttgtgaaaaa cggttacaat ccgccacatg aaaaaacgca ataatcctct
     1921 tcctctgttg aacggtgtca aacccagtta tttggtgctg ccgcatgaaa agcagtttta
     1981 cgggctgccg ctgctgcatt ttctgtgcat ccgctttcct tttgtgggcg cggacgattg
     2041 gcgcaggcgg ttgaacagcg gttttgtggt cggttcggat ggtgcggcgt tggacgaaca
     2101 tactttgttc gagccgggta aggtgatgtt ttattatcgg gaaaccagca gtgaggatga
     2161 gccgcgtatt ccgtttgaag aaaagatttt gcatattgat gagcatttga ttgtggtgga
     2221 caagccgcat tttctgcccg tcatccccag cgggcgtttt ttaagggaaa cgctgctcac
     2281 acgtctgcgc ctgcgtcccg aattgcagca tttgaatgtt gaagacatta cgccgctgca
     2341 ccgcttggac aaggatacgg caggcgtgat gttgttgtcg cacaatcctg ccacgcgcgg
     2401 ggcttatcag acgatgtttc aaaacaaaac ggtatggaaa acgtatgagg cgcttgcgcc
     2461 gacaaggacg gatttgccgt atccgctcga tgtggtttcg cgtttggtga ggggtgagaa
     2521 attttttacg acgcaagagg cggaaggcga accaaacgca catacgacgg tcgaactgat
     2581 tgaaaacagg ggggaattca gcctttaccg ccttacgccg catacgggca agaaacacca
     2641 gttgcgcgtg catatgatgg gcttgggtat gccgctgttg aatgacgcgc tctatcccgt
     2701 gccgtctgaa gcgggcagcg aggattatcg gaaacctttg aagctattgg caaaaaagat
     2761 tgcgtttgca gatcctttgt cgggtcggga aagggtgttt tgcagcggat tttgcctata
     2821 atgagaggga acgcaaaccc gtatcggcgg atgccgccga tacgggtttt acattatcga
     2881 tgccgtctga aaggggtttt atccgttcag gcatacgggc gcgaagcgga ggatggtgtc
     2941 gcgcaggacg atgagtttgc cgtgaaagaa atgcgtggaa ccggcgatgg taatgacggg
     3001 caaatcttgc ggttccgccc atttcagtgc tttttcgatt tcgacgactt catcttccgc
     3061 gccgtgtatc atcagtgttt ttgcaacgtt gggaacggcg gacggctcgg ggcggccggt
     3121 atagtggcag actgccgcgc cgatgagcag caataaatcg ggtatgcgcg cttgtgcggc
     3181 aaatgtggcg acataaccgc cgaaggagaa gccggataag gcaaattcgg gggcttcggg
     3241 atgttgggca cgggcatagt cgatgacggc gaggcagtct tgcgtttcgc cgcgtccgta
     3301 atcgtgtgtg ccttcgctgc cgcctacgcc gcgaaggttg ggcaggtagc agtggaagcc
     3361 gagtttgctt aaggctttgg cggcagtttg gataactttg ttggtgtttg ttccgccctg
     3421 gagggggttg ggatgattga tgacggcaac accgcgtgcc ggtacttgtt cggacgggat
     3481 atggatggtt tctaaaatgc cggcaggacc ggatatgtgt atggtttcgg gtttcagcat
     3541 aatgatttcc tcttgatttt ccgcatgttc cgatgcagcc gtgaagatag ttgcttaaag
     3601 gtggaaatgc ttgccgttgt tggggttgga cgctttcagg ccggcaagca tacggtggaa
     3661 gtccaatccg tatgcgttgc tcagtttgtc ggcacgtttt ttgagtttgt ctatgttttg
     3721 ctctttgggg ctgctttgga aggccattac cgcgacattg ccgtggcttt cggcaggaag
     3781 ttccaagacg cgcccttcaa agacgtttaa caggcgttcg atgaagcgtt ggtagcgttt
     3841 gtcgccgctc caccagttgg tcacgaatat gccgtctgaa gagagtgcgt tgcggcagtc
     3901 tcggaagaac ggttcttcaa ccagcgtatc gatgatttgt tcgccgtcga atccgtccac
     3961 caagatgata tcggtgttgt ggcggaagac tttgatatat tccgcaccgt ctgcttcaat
     4021 aatttcaaat ttctcgccct cgaaaggcaa ctcaaacagg ttgcgggcaa tggcgataac
     4081 ctgcgggttg atatctacag cagtttgacg tgtatcgggc aggtaggtat ctatccagcg
     4141 tgcaaacgaa ccgccgccca agccgatttg ggtgatatgc tgcggaagtg cgtcggtaaa
     4201 cagcagccag cccatcatcg cgcggctgta agagagtacc agctcggacg ggcggtcgag
     4261 gttcatcgag ctttgaacgg tgtcgctgcc caagtgaagc gaacggatat tgccttcttc
     4321 ggaaatgccg acttcgggaa agccggattt tgcaggacgc aggcggcggt agggatgtct
     4381 tgccatcggt gtgaacggtc ggtttgaaag gcggatattt tatcagaatg ccgatgcttg
     4441 tcctgcttca aattaatttc ttttcaaata aattacttat tcggatttgc cggggctttc
     4501 ggataaattc cttgccaagg tgcggcattg cctgcataat tcgctttctt tgccggtata
     4561 gctcagttgg tagagcacct gacttgtaat cagggggtcc cgagttcgac tcttggtgcc
     4621 ggcaccattt ctgctgatgg tgttgcaaaa catttcagta agccggtata gctcagttgg
     4681 tagagcacct gacttgtaat cagggggtcc cgagttcgac tcttggtgcc ggcaccagat
     4741 ttgacagtcc gattgtgtaa aacagtcggg ctgttttgca tttgcggcac ggtcggtggc
     4801 agggctgcgg gcatttcact ataatagccc gtttcaaact gaaaaggccg tctgaaaagg
     4861 gcggggtaac aatatctgat gattactgtg aacacactgc aaaaaatgaa ggcggcaggt
     4921 gagaaaatcg ccatgctgac cgcttacgaa tccagttttg ccgcactgat ggacgatgcc
     4981 ggcgtggatg tgctgttggt tggggattct ttgggaatgg cggttcaagg gcggaaatcg
     5041 acgctgcccg tcagcctgcg cgatatgtgc tatcacactg aatgtgtggc gcgcggtgca
     5101 aaaaatgcga tgattgtcag cgatttgccg tttggtgcat atcagcagag taaggagcag
     5161 gcgtttgccg ccgccgccga actgatggcg gcaggagcgc atatggtcaa actcgaaggc
     5221 ggtgtgtgga tggcggaaac gactgaattt ctgcaaatgc gcggtattcc ggtttgcgcg
     5281 cacatcggtc tgacaccgca atccgtgttt gcttttggcg gctacaaagt tcaggggcgc
     5341 ggcggcaagg cgcaggcttt gcttaacgat gccaaggcgc acgatgatgc gggggcggcg
     5401 gttgtattga tggagtgtgt tcctgcggaa ctggcaaaaa aggtaactga aactgttgcc
     5461 tgtccgacca tcggcatcgg cgcgggggtc gattgcgacg ggcaggtttt ggtgatgcac
     5521 gatatgctcg gtatttttcc gggcaagacg gcgaaattcg tcaaaaactt tatgcagggg
     5581 catgatagtg ttcaagcggc ggttcgggcg tatgttgccg aagtcaaagc caaaaccttc
     5641 cctgctgcgg aacatatttt tgcagactga acaaggtttg ctgctgatgc cgtctgaaag
     5701 ccgtttcaga cggcattttg ttttgccgtg tgcggcgcgt ataatcggcg cgttttgtcg
     5761 ggcaggaagc ccgaaggata aggattaccg ttatgcaaat catacatacc attcaagaac
     5821 tgcgcgcgtg gcgtaaaaat gcgggaaagg tggcatttgt gccgaccatg ggcaatctgc
     5881 atgaagggca tcttgcgctt gtgcgtgagg cgaaaaaacg cgcggacagt gtcgtggtca
     5941 gcatattcgt caaccgcctg caattcggtc agggcgagga tttcgacaaa tatccgcgta
     6001 ctttgcaaca ggatgcggac aagctggaag ccgagggtgt tgccgttgtt ttcgcgcccg
     6061 atgagaaaga actctatccg aacgtggagc aacggtataa cgtcgaaccg cccaacctgc
     6121 aaaatgagtt gtgcggcaag tttcgtccgg gacatttccg cggggtggca acggtagtgt
     6181 ccaagctgtt caatattgtc gcgccggatg ttgcctgttt cggcaaaaaa gactatcagc
     6241 agcttgccgt gattaaaggt ttgaccgagg atttgaattt tgatgttgaa atagtgcctg
     6301 tcgatacggg gcgcgcggaa gacggtttgg cactctccag ccgcaaccag tatttgagtg
     6361 cggcggaacg cgacgaagca ccgcgcctgt accgcgaatt aaaggctgtt gccgaatcct
     6421 tggcgcaggg cagtttggat tatgcaggtt tggagcggca ggctgccgac catttgaccg
     6481 ctgcgggctg ggtggtcgat tatgtcgaaa tccgccgcgc cgatacgctc gaagtggcgc
     6541 gggcgggaga taagaaactg gtggtcttgg ccgccgcccg tctagggacg acgcgcctga
     6601 ttgacaattt ggaaataaaa ctcccttaaa ccgcaagcgt cgggaatgcc gtctgaagcg
     6661 gatttgcgtt tcagacggca tttatttttt tgaacggggt ttcgcaaatc tacaaacgat
     6721 tctgcttgtg ataaagttac gcctgattat atgccgtctg aaggttcgga cggctgtcgg
     6781 ataaaggaag attatgttac ccgcccgttt caccatttta tctgtgctcg cggcagccct
     6841 gcttgccggg caggcgtatg ccgccggcgc ggcggatgcg aagccgccga aggaagtcgt
     6901 aaaggttttc agaaagcagc agcgttacag cgaggaagaa atcaaaaacg aacgcgcacg
     6961 gcttgcggca gtgggcgagc gggttaatca gatatttacg ttgctgggag gggaaaccgc
     7021 cttgcaaaag gggcaggcgg gaacggcttt ggcaacctat atgctgatgt tggaacgcac
     7081 aaaatccccc gaagtcgccg aacgcgcctt ggaaatggcc gtgtcgctga acgcgtttga
     7141 acaggcggaa atgatttatc agaaatggcg gcagattgag cctataccgg gtaaggcgca
     7201 aaaacgggcg gggtggctgc ggaacgtgct gagggaaaga ggaaatcagc atctggacgg
     7261 actggaagaa gtgctggctc aggcggacga aggacagaac cgcagggtgt ttttattgtt
     7321 ggcacaagcc gccgtgcaac aggacgggtt ggcgcaaaaa gcatcgaaag cggttcgccg
     7381 tgcggcgttg aaatatgaac atctgcccga agcggcggtt gccgatgtgg tgttcagcgt
     7441 acaggggcgc gaaaaggaaa aggcaatcgg agctttgcag cgtttggcga agctcgatac
     7501 ggaaatattg ccccccactt taatgacgtt gcgtctgact gcacgcaaat atcccgaaat
     7561 actcgacggc tttttcgagc agacagacac ccaaaacctt tcggccgtct ggcaggaaat
     7621 ggaaattatg aatctggttt ccctgcacag gctggatgat gcctatgcgc gtttgaacgt
     7681 gctgttggaa cgcaatccga atgcagacct gtatattcag gcagcgatat tggcggcaaa
     7741 ccgaaaagaa ggtgcttccg ttatcgacgg ctacgccgaa aaggcatacg gcagggggac
     7801 ggaggaacag cggagcaggg cggcgctaac ggcggcgatg atgtatgccg accgaaggga
     7861 ttacaccaaa gtcaggcagt ggttgaaaaa agtgtccgcg ccggaatacc tgttcgacaa
     7921 aggtgtgctg gcggctgcgg cggctgtcga gttggacggc ggcagggcgg ctttgcggca
     7981 gatcggcagg gtgcggaaac ttcccgaaca gcaggggcgg tattttacgg cagacaattt
     8041 gtccaaaata cagatgttcg ccctgtcgaa gctgcccgac aaacgggagg ctttgagggg
     8101 gttggacaag attatcgaaa aaccgcctgc cggcagtaat acagagttac aggcagaggc
     8161 attggtacag cggtcagttg tttacgatcg gcttggcaag cggaaaaaaa tgatttcaga
     8221 tcttgaaagg gcgttcaggc ttgcacccga taacgctcag attatgaata atctgggcta
     8281 cagcctgctt tccgattcca aacgtttgga cgaaggcttc gccctgcttc agacggcata
     8341 ccaaatcaac ccggacgata ccgctgtcaa cgacagcata ggctgggcgt attacctgaa
     8401 aggcgacgcg gaaagcgcgc tgccgtatct gcggtattcg tttgaaaacg accccgagcc
     8461 cgaagttgcc gcccatttgg gcgaagtgtt gtgggcattg ggcgaacgcg atcaggcggt
     8521 tgacgtatgg acgcaggcgg cacaccttac gggagacaag aaaatatggc gggaaacgct
     8581 caaacgtcac ggcatcgcat tgccccaacc ttcccgcaaa ccccggaaat aatgcaggtc
     8641 catcctttca gacggcataa ggtttgccgg gaagctgcgg cattcgggca aacggcacgc
     8701 agttcgcacg cgttttgcac ggcacgccga acccatcggc cggcaggatg gcacccgtta
     8761 aggaaattct gatgaaacac accgtatccg catcggtcat cctgcttttg accgcttgcg
     8821 cgcaattacc tcaaaataac gaaaacctgt ggcagccgtc cgaacacacc cgcagtttca
     8881 cggcggaagg gcggttggca gtgaaagcgg aagggaaagg ttcgtatgca aatttcgatt
     8941 ggacatacca accgcccgtg gaaaccatca atatcaacac ccctttgggc agtacgctcg
     9001 ggcagttgtg tcaagacagg gacggcgcat tggcagtgga cggcaaagga aatgtctatc
     9061 aggcggaaag tgcggaagaa ttaagcaggc agctggtcgg tttcaaactg ccaatccaat
     9121 atctgcatat ctgggcagat ggcaggcgtg tggcgggcgc gccttaccgc atcctgccgg
     9181 acggcatatt ggaacaatac ggttggactg tcggcagaac cgccgacagt ggggggcaag
     9241 tccgaacgtt gcaactgaat aacggaaatt tgaacatcag gctggttttc accgaaatcg
     9301 gtatgccgtc tgaaaccgaa accccggaac gctgtgcggc gcgcacgaga taaggcggac
     9361 agatgaatat tgcggacgga cggcaggcgt ttcccgcacc tgcaaaactg aatctcgatt
     9421 tgaggattac tggaaagcgg gaagacggtt atcacaatat cgaaagcata ttctgcctga
     9481 tagatttgca ggataccgta tatttgaaac cgagggacga cggcaaaatc atcctgcaca
     9541 atccggttga tggcatgccg caggaagtag atttgagcta ccgtgccgca tcgttgctgc
     9601 aaaaatatgc gcgcaacccc gccggcgtgg aaatatggct ggacaaaaaa atcccgacag
     9661 gggcgggttt gggcggcgga agctcggatg cggcaaccgt tttgctggtg ttgaaccgtt
     9721 ggtggcagtg cggtctgacg cagcggcagc tcattgattc gggcgcggct ctgggagcgg
     9781 atgtaccgtt ttttattttc ggcaaaaatg cgtttgcgcg gggtataggc gacaggctgg
     9841 acgaaatgga tattccgaaa cagtggtatg tcatcgtcaa accgcccgtc cacgtttcca
     9901 ctgcaaaaat tttcacacac gaaggcttga cacgaaattc cgcctcaagc ataatgccga
     9961 ctttccaaaa cctgcaaccg tttagaaatg acatgcaggc agttgtattt aaagaatacc
    10021 ctgaagtttg gaaagcctat tccgaattgt cccgatatgg atttgcctta atgacaggtt
    10081 ctggtgcgtg tgtattcacg gcgtgtcaag ataggaatag cgcatacaat atataccgac
    10141 aagtttcaga tttgtacgag gcatatttgg cagagggtct ttcaaaacat cctttgttgt
    10201 ccgtataaac attgttgggg agtcgtcaag cggttaagac actggatttt gattccagca
    10261 tgcgaaggtt cgaatccttc ctccccagcc aagtcaaacg agttggggag tcgtcaagcg
    10321 gttaagacac tggattttga ttccagcatg cgaaggttcg aatccttcct ccccagccag
    10381 ccctttttgg ggagtcgtca agcggttaag acactggatt ttgattccag catgcgaagg
    10441 ttcgaatcct tcctccccag ccagataaaa gcgtgtaagc ctgcttacac gcttttattt
    10501 catagaaata aaaatattga aatgcctttg tttgtgtcgg atgttgcagg tataatgtcg
    10561 ggcttggtac aagcagaggg aagcattgtg ttttctgagc ggaagttaaa cataaaatca
    10621 ggtgagaata tggctgcgta cgacagtttg atggtattta caggcaatgc caatcccgaa
    10681 ttggcacaac gtgttgtcag gcatttggac atttctttgg gcaatgcttc cgtatccaag
    10741 ttttcagacg gcgaagttgc cgtcgaactg ttggaaaacg tacgcgggcg cgatgttttc
    10801 atccttcagc cgacctgtgc gccgaccaat gacaacctga tggaaatcct gacgatggcg
    10861 gatgcactga agcgtgcttc ggcaggtcgt attaccacag ccattccgta tttcggctat
    10921 gcgcgccaag accgccgtcc gcgttccgtc cgcgttccga tttctgccaa actggtggca
    10981 aatatgctgt attcggcagg gatcgaccgt gttttgactg tcgatttgca tgccgaccag
    11041 attcaaggtt tcttcgatat tccggtggac aatatttatg ccaccccgat tctgttgaac
    11101 gacatcaaac aacagcggat tgaaaatctg accgtcgtca gcccggacat cggcggtgtc
    11161 gtccgcgccc gcgccgtggc aaaatccctg aatgccgact tggcaatcat cgacaaacgc
    11221 cgcccgaaag ccaatgtggc ggaagtcatg aacatcatcg gcgatattca aggtagaacc
    11281 tgtctgattg tggacgatat gattgacact gcaaatacgc tgtgcaaagc cgccgtcgcc
    11341 ctgaaagagc ggggggctga acgtgttcta gcctatgcca gccacgccgt attctccgga
    11401 gaggcggtca gccgtatcgc ctcatccgaa atcgaccagg tggtcgtaac cgataccatt
    11461 cctttgtctg aagcggctaa aaactgcgac cgtatccgtc aggtaacgat tgccggtctg
    11521 ttggccgaaa ccgtccgccg cattagcaat gaagaatccg tctcatatct tttcaatgaa
    11581 gaagtgatga caggcagcat gttgctgcca taagcccgaa gccgtcttaa gctggtcgcg
    11641 gccgatgacg gcgattttac ctaacttgga gtatttaaca tgacttatga aattcaagcc
    11701 tctgttcgtg aagcacaagg cactggtgcg agccgccgcc tgcgtcgcga aggccaaatc
    11761 cccggcattc tgtacggtga aggtcaagag cctgttgcaa tcgctgtgga tcacaaaacc
    11821 gtattctacg cattggaaaa agaatctttc catactgcgt tgattaagtt gtctctgaac
    11881 ggtgaaacca aagacgttat cgtccgcgat ttccaaatgc acccgttccg ccgcgaagtt
    11941 caacacatcg acttccaagc tgtgaaagca gaccaacccg tacgcatccg cgttcccctg
    12001 cacatcgtta acgctgaaaa ttcccaagcg gtcaaactgc aaggcggccg cgtatctctg
    12061 ttaaacactt ctgttgaagt agttgctttg cctgccaaca tccctgcttt cttggatttg
    12121 gattgtgccg aagtggttgc cggcgacatt ctgcacttgt cagacatcaa actgcctgaa
    12181 ggtgtagaaa gcgtttccct gaaactgaaa cgtaacgaaa atctggctgt tgccaccgtt
    12241 accggtaaaa aacgctaatt gattgaaaag caggcggtac gtatatgatg cgtaccgcct
    12301 tattgttttt ggctgtgttt caatatttgg ttgatttcat ccgtttataa gggaaaaccg
    12361 cttatttccg ttcgggtgga aatttatcca gacgtattgc gcgacaacgg atggttggct
    12421 caaaaacggc atttcgtacc ggatgaaaat gaagaagata tgtcgttcac attccggtag
    12481 ctgtccggct cgaaccggct atcagagata gtcggtcaat tttcaccctg aaaatcactt
    12541 ttcttttaac ttgcaacata ttccgataaa atggacactt tgtaagtaat cattgatgga
    12601 agacgcaatt atggcaaccg aaccgaaccg aaccgaaccg aaccgaaccg aaccgaaccg
    12661 aaccgaaccg aaccgaaatt atacacgatt tagaagataa tttcaaacaa aacaatatat
    12721 taaaactcaa acaactcttt cccgaagtct tttgtgaaga ccaaattgac tttgaaaagc
    12781 tcaaacttgt cctcggcgca gaaaaccttg ccggtgcggg cgaacgttac caactcgatt
    12841 gggcgggcaa aaccgcctcc tatctcaacc tgcaaagtcc cacttcccgc accctcatcc
    12901 cctgcaaaga agaatccgca gatttcgacg gcacgcaaaa cgtgtttatt gaagccgaaa
    12961 acctcgaagc cttgaaaatc ctgcaaaaat cctacgctgg cagcgtgaag atgatttaca
    13021 tcgacccgcc ctacaatacc ggcagcgatt cctttatcta tcccgacaaa ttctccgaaa
    13081 gccgcgaaga atacgcccgc cgcgtgggcg acaccgacga cgcgggctac ctgaagcgcg
    13141 acggcgtatt ccaaggcgcg tggcgcaaaa acggcaaaga cagcggccat taccacagca
    13201 actggctctc catgatgctg ccgcgcctgc atctggcgaa aaccctgctg cgtgaagatg
    13261 gtgtgatttt catcagtatt gacgacaacg aacaggcaca gttgaaattg ttatgtgatg
    13321 aagtgtttgg ggcggagaat tttgtcacaa acattatttg gcaaaaaaaa tatcgcccgc
    13381 aaaatgatgc cgctaatttt tcagaagagc atgattttat tttatgttat gcaaaaaaga
    13441 aaaaaagaaa aaaatctgac tatgggtggg aaagaaattt gttacaaaga acagcagagc
    13501 aaacttctga ttataaaaat cctgataatg atcctcgcgg agcttggtct tcaagtggct
    13561 tgatgattga aggtacagct aaaaattgta tatatcctat tgttggtccg acaggaatag
    13621 agttttatcc aagtgctggg aaaagatggt caattagtca agataatttt gagaaactta
    13681 attcagaaaa tcgtatctgg tatggaataa acggtaatgc tgctccacgt gtaaaacgat
    13741 atttaagtga agtgcaagat gggattgtgc caagtacaat atttttattt tctgatgtag
    13801 gacatactgg tgaaggaaaa aaagactttc tgaaaatatt ttcagaagaa ctatttgatt
    13861 atccaaaacc aatccgtcta ataactaaac tattaacaat tggttcaaat tcagacgacc
    13921 taatcctcga cttcttctcc ggcagcggca ccaccgccca tgccgtgatg cagcttaacg
    13981 ccgaagacgg tggcagccgc cgtttcatct gtgtccagct tcccgaagaa accgacgaaa
    14041 aatccgaagc gcgcaaggcg ggtttcaaca cgattgccga aattgccaaa gaacgcatcc
    14101 gccgcgcggg caggcagatt tcagacggcc ttcaagacgg atcggaaata gatacgggtt
    14161 ttaaagtttt caaacttgct gaaagcggtt tcaaacaatg gcggcagccc gagcagtccg
    14221 atacggaagc cctgcaaggc gagttgtcct tgaacattga ttccgtgttg tctgaaatgc
    14281 cgtctgaaaa cctgctctac gaattgatgt tgcgtttagg tttgaagctg acttgcaaga
    14341 tttcgctttc agacggcatg tatttcgtgg aagacgagga tacgggcggg ctgtacgctt
    14401 tcctgctgga acgtgtggat cagggtttga ttgatgccgt gctggcgaag catcccgtca
    14461 aggtggcggt tttggacagg ctgttcgacg gcgacgacgc gctcaaaagc aatactgtat
    14521 tgcagatgaa ggacgcgggc gtggtgttcg agtgcgtgta gggagaaaac gatggaactg
    14581 aaatttgaag aactgcccta tcagcttgat gcggtaaatg ccgttacgaa tctttttgca
    14641 gggcagccga accacactcg gacattcgat ttgaccagtc aggggacggg gcggtttgtc
    14701 ggcaacggct tggatttgga ttgggaaacg ctaggccgga atttgaatgc gttacaaaag
    14761 caaaacggac agcctgaaac ggaaatcggc gcgcacgggc tgaatttttc cctggaaatg
    14821 gaaacgggga cgggcaaaac ctatgtctat ctgcgcacga tttacgaatt gaacaaactc
    14881 tacggctgga agaagtttgt gattgtcgtg ccgagcgtgc cgatacgcga aggcgtgttg
    14941 aaaagcctgg aaattacgaa agcgcatttt gacggggtgt tcggcaggcc cgtgatgcgt
    15001 tatgcggaat attcaagcag ccgactgtca gacttgcgcg cttttgcggt aaacgaccac
    15061 atcgaaattt tagtgatcaa catccagtcg tttgaaaaag acggaaacgt tatcaataag
    15121 gtaaacgaaa gcggggaagc accgattaag tttattcagg aaacgtctcc catcgtcatc
    15181 atcgacgagc ctcagaatat ggaaacggaa ggccgtctga acgctttgga atcgctccat
    15241 ccgctcttta ccctgcgcta ttcggcaacg cataaaaacc cttaccacaa ggtgtacagc
    15301 ctgaatcccg tgcaggcata caatttgaag ctggtcaaac agattgaagt cttgccagta
    15361 ttggcggaaa acgatgtgaa cggcgcattt gcggagctgc tggaagtaaa gcaggcggcc
    15421 aaaggccgtc tgaaagccga tttgaacatt cattatcagg ataaaaagga aatcaagaag
    15481 aaaaaggtat ccgtcaaatc gggagatgat ttgtttgaaa aatcgggcgg caacgaagcc
    15541 taccgccacg gtttcatcat cgacggcatg gatttcgaga atcaggaatt ggcgttctca
    15601 agcggtaaaa ccattacgcg cggcggagac ggcgacgtag tgcgcgatga aatcatgaaa
    15661 aaccaaatcc gcgaaacggt aagggagcat ttggcgaagg agaaacggct gaatccgctg
    15721 gggattaagg tgttgtcgct gttcttcatc gaccgcgtag agaattaccg tgcggcagac
    15781 ggaacagcgg gcaagtttgc cctttggttt gaagaaatct atcgagagct tgtaggcagt
    15841 accaatacgg caggcgtaca cgacggctat ttctcgcaag acaagaaagg ccgtctgaaa
    15901 aacaccgacg gcacaacgca ggcggacaac gatacctacc gcctcattat gcgcgacaag
    15961 gaaaccctgc tttcctttga tagcccgctg cgctttatct tttcccattc cgcactgaaa
    16021 gaaggttggg acaaccccaa tgtattccaa atctgcacgc tcaacgaaac ccgctctccg
    16081 ctgaaaaagc ggcaggaaat cggacgcggg ctgcgtatag ccgtcaatca ggaaggacgg
    16141 cgcgtgcgcg atgaaaaagt gaacatactg actgtagtgc cgaacgaaag ctacgaattg
    16201 tttgccgcaa atctgcaaaa ggaatacgaa gatgaatgcg gtattaaatt tgccgtcgaa
    16261 aacatcaaaa acggcaataa gaagaaaaaa cagctattcc gcaaagattt tccgctcgat
    16321 cctgaatttc aggcaatttg ggagaggttg aaatacaaaa cgcgctaccg ggtgcgcttt
    16381 gatacggaag aattgattaa acaagcttcg gaggcagtag caaacttgcc agaaatccaa
    16441 aagccgagaa tccgcgtccg caaggcgcaa ttaaaacaat cacaaaccta cggtatcgaa
    16501 acagttgaaa ttgccagccg cagccgtgaa atggacgttc aatgggatat cccggacata
    16561 ctcggcgaga ttcaaaaaaa gacggggctg acgcggcgca cggtgtacga aatcatcaca
    16621 cagtcaggcc gtctgaaaga tgtagctggt aatccgcaac gcttcatcga ccttgctgcc
    16681 gaagcggtaa accgcagctt gtacgcgttg atggcggcag gaatcgagta tgaacaaacc
    16741 ggagaagtgt acgaccaaaa gctgttggaa gacatggagc gtgaaggcat cgagttttat
    16801 gaaaacagct ttacgttcga agtcggaaaa ccgcaaaaaa ccatttgcga aaactatatc
    16861 cccttggatt ccggcacgga acaagccttt gcccgaaatt gcgaggatta tgatgccgaa
    16921 aatgaaggcg gcgtggcgtt ttacttcaaa ctgcccggct ggttcaaaat cccgaccccg
    16981 ctgggcaatt acaatcccga ttgggcggtg gtgaaacagg ttcaggacaa agtgtatttc
    17041 gttgcagaaa ccaaaaatac cggcaaaagc attcaggcgg gggtagatgc gggcaagctg
    17101 cgcgaaaacg aacggctgaa aatcgaatgc ggtgaacggc attttgaagc actgggcggt
    17161 ttggcatact gtgttgtgga aaaagtgggg gacttaaagt tagaaaaaga ggagcaggct
    17221 taaaacgaac aggtgtaatc atagtatggt gatgtagtgc ttggggcggg cggcaactga
    17281 agatgcggat gcggcaatta ccttgattca ctaaaccgat tgggggttga agattgtttc
    17341 agacggcatt ttaattctcc tatcttgggc atggaaatat agaagcttac tgtcccgtca
    17401 ggcgtgccca gagttttttc caccagtttt tcttttctac gtcctccagt gcgacaattt
    17461 ctttttcggt aatggtgtat ccgttttgcc tgattttgat ttttcctaaa atctgtccct
    17521 tttttaccgg ggcgggaatc ggctgtatgg tttctagaat ttgttctgcc attttcgctt
    17581 ccttatgtgg cagagtgatg taggcttctt tgaggaagcc tgcgcggacg gtttttttgc
    17641 tgcctccgga aatttggatt tgggcaacgg ttttgccttt cggatatatt ttgggcgtat
    17701 cgaaggcctg caatgcccag ttcagcagct tgctgttgtc tgatgcgcgt gtttccgccg
    17761 attccgaacc caatgtgatg acaaggatgt gcctgccgtt gccggagtat gacacggcaa
    17821 ggttgtagcc gccgctttct gtgtgtccgg ctttcagacc gtttacattg ttgtccctat
    17881 ataaaaggat attgcggttg ttttgctcta tatttttgaa tttgaaagat ttgatggaaa
    17941 acagcgggta atattccgga aagtcgcgca tcaatgcttc agacagctgg gcgaggtctt
    18001 tggcggtgga aacctgtcct tctctactca agcctgtcgg atttttgaat acagtgttct
    18061 tcatgcccag gcgtcgggct tctttgttca tttgttgcac aaaattttca atcgagccgt
    18121 tgcccagccg gccggcaagg gttagggcgg catcgtttgc ggatagtgca atcatgcctt
    18181 ttaagagttt gtcggtgctg accgtatcgc cgggacgtac aaacattctg cttccttctg
    18241 aagcccatgc ggattcgggt atttttaagt tttcttcaga tcggatattt cccgatttca
    18301 tgtttttgaa aaccagatat gcggtcatca gttgggttag tgccgccggt tcgacagggg
    18361 tattgatgtt tttggcggat aaaatctgtt tgctttgaag gtcgataacg atgtgtgccg
    18421 ctgtgagggt ttcgggtgtt tggaacgtgg gggcggcgtg taccgccggt ttgttgggcg
    18481 cgggcgatgc ggccgttgcg tgagaaacgc ctaagatgat ggaaagcagg acgggcagga
    18541 ttttatgtgc tgtcatgaaa tattctaatt gtgtgcgtgt ttcagtctgc cgattatacg
    18601 cttagggtgt ctgatcgggc ggatttttct tgctttcgcg ccgtcttggg cgtatggttt
    18661 tgggttttgc gattttaata aaccgattat cccatattga attatgaaca cgccccttcc
    18721 ttattccaat tacctcatcc gcatcctgac ggcatctgtc tatgatgtcg cggtcgaaac
    18781 gcctttggaa ccggcacgca gcctttctgt acgtttgaaa aacaacatcc ttttgaaacg
    18841 cgaagatttg cagccggttt tttcgttcaa aatacgcggc gcgtacaaca aaatgtccaa
    18901 gttgccgaaa gatgcgctcg cttgcggcgt gattgcggca agcgcgggca atcacgcaca
    18961 aggcgtggca ttgtccgcac agcgtttggg ttgccgtgcc gttatcgtca tgccggagac
    19021 tacgccgaaa atcaaagtgg atgcggtcaa aagccgtggc ggcgaggtgg ttttgcgcgg
    19081 tgtttcatac aacgatgctt acgattatgc gatggagctg gcggaacagg aagggctgac
    19141 ctatatcgca ccgtttgacg atcctgatgt gattgcagga caagggacgg tagggatgga
    19201 aattgtcagc cagcatcccg atccaatccg cgccgtattc gtaccgatag gcggtggcgg
    19261 tttggcggcg ggcgtggcgg catttatcaa gcaggtccgt cccgaaatca aagttatcgg
    19321 cgttcagacc aacgattcct gctgtatgaa gcagtcggtc gaagcgggtg aaatcgtcca
    19381 tttgaaagat gtcgggctgt tttcagacgg cacggcggtc aaagtcgtcg gaaacgaaac
    19441 cttccgtctc tgcaaagaac ttttggatga aatcattaca gtcgataccg atgcggtttg
    19501 cggcgcggtc aaggatattt tcgatgacac gcgcagcatt accgagccgg cgggcgcgtt
    19561 ggcgttggcg ggtctgaaag cctatatcgc ccgagaaggc gcggaaaacc aaaccctgat
    19621 tgccgttacc agcggtgcga atatgaattt tcaccgtttg cgccacgttt cggaacggag
    19681 cgaattgggc gagggcaacg aaggtatttt tgccgttacc atccctgaag aacgcggcag
    19741 cttccttaag tttgtcaata tattgggaaa taggaatatt accgagttca actaccgcta
    19801 cggggacgat gaaaaagcgc atatctttgt cggacttcaa gcggcaggcc cgcaggattt
    19861 ggcggttatc ggcagccggt tggatgaggc gggattgccc aatgtcgacc tgaccgatga
    19921 tgagattgcc aaaatccata tccgctatat ggtcggaggg cggacggaca aagtagaaaa
    19981 cgagcgtctg gtcagttttg agtttccgga gcgtccgggg gcgttggcgc gttttttgaa
    20041 ccatatgcag ggcggttgga acattacgct cttccattac cgcaaccacg gtgcggatta
    20101 cgggcggatt ttggtcggta tcgatgtgcc gccgcacgat gccgccgcat ttgacggttt
    20161 cttggaaagt ctgggataca gctatcacga ggaaacgcaa aatgccgcgt acaagctgtt
    20221 tcttgcctga cgcttgaaag cacaatgccg tctgaaagcc tttcagacgg cattgcgctt
    20281 tcatggttaa atcgaatatt caatcagttc gttttgagag aagacataaa cctgtttcgg
    20341 aatcagcttc aattctttgc cttcggcgat tgggtaacgc gcggcatcgc tgccagccag
    20401 cgtgatatgc acgtcttgtt tgccgtgttt caccaaaata tgcgtcaatg ccccgacggc
    20461 gtggactttt tcgatttcgg cacaaatcat cggtgtttcg tgttcggcgg cgatctgcca
    20521 ttcgtgcggg cggatatagc cgatggcggt ttgctcctgc catttgtagt gcgcgtccaa
    20581 tttccacgcg aagccgttgt agtgccagat gcctttttcg atgcgtcctt caaaagcgtc
    20641 ggtttcaccg aggaactcgg taacgaaggc gttttcgggt ttgcggtaaa tggcttcggc
    20701 gctgccggtt tgttcgattt tgccgtggtt catcacgacg atttcgtcgg aaacttcgag
    20761 ggcttcttct tggtcgtgcg taaccagaat gctggttaca cccaggttgt gatggatgtc
    20821 gcgcagccag gtgcgtaatt ctttgcgtac tttggcatcc aacgcgccga agggttcgtc
    20881 caaaagcagg agtttcggtt cgaccgcaag cgcacgggcg agggcgatgc gctggcgttg
    20941 cccgccggag agttggtgcg gataggattt tgccaaatgc gagagttgta cgagtttcag
    21001 caattcttcg actttggcgc ggatttgtcc tttggacggg cgttcggact tgggcaatac
    21061 ggtcaaaccg aaggcaacgt tgtcaaacac gttcatatgg cggaaaaggg cgtagtgttg
    21121 gaacacgaag ccgactttgc gctcgcgcac gtgcttggcg gttacgtctt gcccatcaaa
    21181 cagaatcttg ccgccgtcgg cgttttccag tccggcgata atgcgtaaaa gtgtggtttt
    21241 gccgcagccg gacgggccga gcagggaaac gagtttgccg gtggggacgt tgaggttgat
    21301 gtttttcagc gcgtgaaaat tgccgaagtg tttgtttaag ttttgaatgg tgatgctcat
    21361 attgcgttcc tttcggcggc ggcgagtttt ttgtcttgta atttggtgat gatgttctgc
    21421 accgccagcg tcgccagtgc taaaagtgcc aatacgccgg agagggcgaa tgcgccggtg
    21481 aagttgtatt cgttgtagaa gatttcgacc aaaagcggga cggtgttggt ttcgccgcgt
    21541 atgtgtcccg ataccacgct gaccgcgccg aactcgccca tcgcgcgggc gttggtaagg
    21601 atgatgccgt agagtaacgc ccatttgatg ttgggcaggg taacgcgcca aaacatctgc
    21661 cagccgcttg cgccgagtat caatgccgcc tgttcttcgc tgtcgccctg tgcctgcatc
    21721 agcgggatga tttcgcgtgc gacaaaaggg aaggtaacga acagcgtcgc caaaataatg
    21781 ccggggatgg cgaaaataat ctgtatgcct tgcgcttcga gccggccacc caatgccgtg
    21841 tgcgcgccga acaataagac gaacatcaaa ccggctacca ctggcgatac ggaaaacggc
    21901 aaatcgagca gggtggtcag caactgcttg cctcggaagt caaaacgggt cagcagccac
    21961 gccatcgcca cgcccaagac ggcattgacg gggacgacaa tcagcgcggt aatcagcgtc
    22021 aatttgatgg cagaccatgc ttcggaatcg cttaaggatt tcaggtacaa atcccaaccg
    22081 ccttttaagg cttcgtaaaa cacggcgacg agcggcacga ccagcatcag cagcagaaag
    22141 cccaaggcgg cggtaatcag caacacgcgc agccggcgcg gttcggtcag gttgggattg
    22201 gcggaatagg gtttcatggc ggtggtgttc cccggttggt tgttttaagg tgttcggatg
    22261 ccgtctgaaa aaccgttttt tgtggggcgg attcgttttc agacaacctt ttattgatta
    22321 agggaataaa aacgtctagc taccgtcatt cccgcgcagg cgggaatcca ccacagggca
    22381 acaggaaaac agaaaataaa taaggcagcc gaaattcacc aatggattcc cgcctgcgtg
    22441 ggaatgacgg taacaggtat ttcagacgac ctcaaccttt cgcgcccgaa cgcctgccca
    22501 acgcccactg catcacgttc agtgcaaaca gaatcacaaa cgaaaccagc agcataaaca
    22561 acgccaccgc cgacgcgccc tgcacgtcga actgttccag cttgcccgta ataatcagcg
    22621 gcaggatttc agaaaccatc ggaatattgc ccgcgataaa aatcaccgag ccgtattccc
    22681 ccgttgcccg cgcgaacatc attcccgcgc cggtcaagag tgccggcgtg atttccggca
    22741 gcagcacgcg gcggaacgtc gtaaagcggt ttgcgcccaa agtcgccgcc gcttcctcat
    22801 attcgcccga taattcctcc aacaccggct gcacggcgcg gacgataaag ggcaggctga
    22861 cgacgaccag cgcaatccaa atgccgacgg gcgtaaacgc gattttgatg cccaaaggct
    22921 cgaaaaaacg gcctatccaa ccgttgggcg catacagggt cgccaacgcg atacccgtaa
    22981 ccgccgtcgg cagcgcaaac ggcaaatcga ccagcgcgtt cgccagaccc ttgcccggga
    23041 attcataacg caccaatacc cacgccacca gcgtgccgaa cacgacattg gtcagcatcg
    23101 cataaaacga catccgcaaa ctcagccaca ccgccgccaa tacgttcggc tcggcaatcg
    23161 tgttccaaaa tccgctccag ccgatttcca ccgccttcgc cgccatcatc gcaaacggca
    23221 agaccacaag cagcgacagg cacaatacgg tcagaccaag gctgagtttg aagccgggca
    23281 gtacgccggg cgttttgagc gctaacataa aacaatgctg aaaataagga aaaggaagga
    23341 ctactttaac gatgccgtcc gaaaaacgga aagaatggaa agtttggtgc aaagacgaat
    23401 ttgttataaa gcggttggca gtttctcaag cgggcgcgat gttttaaaat atagtggatt
    23461 aaatttaaat caggataagg cgacgaagcc gcagacagta caaatagtac ggcaaggcga
    23521 ggcaacgccg tactggttta aatttaatcc actataacgc cttgttttga cggaaaacca
    23581 tcatataaag gaacacttat gcagatttta tcttttcagc cggacattgc ggaacgtatg
    23641 ctggaaggta cggaaggaga gtcggttcat cgggatgcgc agttcgttca aacggcaaac
    23701 ggctattgga ttgcgtggca tgaaggcgta gcggcactgc ttgcgcccga tacgccgccg
    23761 gggattccct gtttttgggt ggaaggcgcg gaaagccttg aagagttgtg cgtcatggtg
    23821 gaacgcggcg agtttgacga agtggaagag tttgacggcg atgacgacga atggctcgaa
    23881 acggcacagg gttgcgggca ccacggcgac gcttgcgcct gcggacatta aaggcattgc
    23941 aggcttgccg caaggggggc aaggctttgc cgttttttaa ataatcggtg gattgcatct
    24001 gtttgcaatg ggcggaacgc ttccctttgt attttattga ataaaaaaac ttatcttggt
    24061 attataatta aggcagcatc aattattttg ggatggcaat aaacgcaaag cattgatttg
    24121 cgccgattgc agacttatta tagcaggttg cggcgcggac ttaatgggaa ttgtttatag
    24181 agcatgatgg tctgaaacat cattaattaa aaataccatt aatccgattt atatttattt
    24241 caatttcaat ggaaaaacat caatgacaat gattttaagc attttaagcc tgttttttat
    24301 catcagactg ttatttttag ccgtctctat taaacatgaa aaagccttga ttgccaaagg
    24361 ggcgaaacaa tacggaaaaa ccaattccac gctgcttgcg gcagttcata cgctttatta
    24421 tttggcgtgt tttgtttggg tatggctttc tgacactgct tttaatggca tatccttgat
    24481 tggtacgctg acggtgatgg cttcgtttgt gatattgtca ttgattatta agcagttggg
    24541 ggagatttgg acggttaaaa tctatatttt accaaatcat caaattaatc gttcgtggtt
    24601 gtttaaaaca ttccgccacc ccaattattt tttaaacatc atacccgaac tgattggcat
    24661 cgccttatta tgtcaagcgt ggtatgtttt attgattggc ctgcccattt atttgctggt
    24721 cttatttaag cgtatccgac aagaagaaca ggcgatggca acactttttt aacccgtttc
    24781 atcaattata gtggattaac aaaaaccagt acggcgttgc ctcgcct
//
LOCUS       DAXHKX010000011        23590 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHKX010000011 DAXHKX010000000
VERSION     DAXHKX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23590
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.011"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(65..904)
                     /locus_tag="ACFMWZ_000413"
     CDS             complement(65..904)
                     /locus_tag="ACFMWZ_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC5934423.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1189..1264
                     /locus_tag="ACFMWZ_000414"
     tRNA            1189..1264
                     /locus_tag="ACFMWZ_000414"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Arg,seq:ccg)
     gene            complement(1329..2522)
                     /locus_tag="ACFMWZ_000415"
     CDS             complement(1329..2522)
                     /locus_tag="ACFMWZ_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC5934424.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVRRAETARAATPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2543..3631)
                     /gene="trmA"
                     /locus_tag="ACFMWZ_000416"
     CDS             complement(2543..3631)
                     /gene="trmA"
                     /locus_tag="ACFMWZ_000416"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC5934425.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3774..4874
                     /gene="aroC"
                     /locus_tag="ACFMWZ_000417"
     CDS             3774..4874
                     /gene="aroC"
                     /locus_tag="ACFMWZ_000417"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC5934426.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLALTESDIQAD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGCEHISQNPFFAANQSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGDPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4966..5370
                     /locus_tag="ACFMWZ_000418"
     CDS             4966..5370
                     /locus_tag="ACFMWZ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC5934427.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLITALPQYDPTLIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(5444..7429)
                     /gene="parE"
                     /locus_tag="ACFMWZ_000419"
     CDS             complement(5444..7429)
                     /gene="parE"
                     /locus_tag="ACFMWZ_000419"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC5934428.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDDHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKNKPARKFYA
                     LDQNELDSILERLQKEGFKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7496..8044)
                     /locus_tag="ACFMWZ_000420"
     CDS             complement(7496..8044)
                     /locus_tag="ACFMWZ_000420"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC5934429.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQPSGNR"
     gene            8185..9480
                     /gene="serS"
                     /locus_tag="ACFMWZ_000421"
     CDS             8185..9480
                     /gene="serS"
                     /locus_tag="ACFMWZ_000421"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC5934430.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYEFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9553..10551)
                     /locus_tag="ACFMWZ_000422"
     CDS             complement(9553..10551)
                     /locus_tag="ACFMWZ_000422"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC5934431.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10676..11752)
                     /gene="prfA"
                     /locus_tag="ACFMWZ_000423"
     CDS             complement(10676..11752)
                     /gene="prfA"
                     /locus_tag="ACFMWZ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC5934432.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDTDDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11842..12612)
                     /locus_tag="ACFMWZ_000424"
     CDS             complement(11842..12612)
                     /locus_tag="ACFMWZ_000424"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC5934433.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(12727..13716)
                     /locus_tag="ACFMWZ_000425"
     CDS             complement(12727..13716)
                     /locus_tag="ACFMWZ_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC5934434.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     VKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGRLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13783..14466)
                     /locus_tag="ACFMWZ_000426"
     CDS             complement(13783..14466)
                     /locus_tag="ACFMWZ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC5934435.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14655..15989)
                     /gene="glmM"
                     /locus_tag="ACFMWZ_000427"
     CDS             complement(14655..15989)
                     /gene="glmM"
                     /locus_tag="ACFMWZ_000427"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC5934436.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYFDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLNLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16119..16970)
                     /gene="folP"
                     /locus_tag="ACFMWZ_000428"
     CDS             complement(16119..16970)
                     /gene="folP"
                     /locus_tag="ACFMWZ_000428"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC5934437.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSSEEEWARVEPVLVEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQTDMGICLMHMQGLPETMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMIGELTGETDAAARVHGSVAAALASVARGAQIVRVHDVKATADALKAW
                     EALGINL"
     gene            complement(17110..18003)
                     /locus_tag="ACFMWZ_000429"
     CDS             complement(17110..18003)
                     /locus_tag="ACFMWZ_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC5934438.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLVEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18094..20208
                     /locus_tag="ACFMWZ_000430"
     CDS             18094..20208
                     /locus_tag="ACFMWZ_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC5934439.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTIGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20273..21751)
                     /gene="ubiD"
                     /locus_tag="ACFMWZ_000431"
     CDS             complement(20273..21751)
                     /gene="ubiD"
                     /locus_tag="ACFMWZ_000431"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC5934440.1"
                     /translation="MKYKDLRDFIVMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     SALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTSYP
                     VSVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVDNTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(21771..22286)
                     /locus_tag="ACFMWZ_000432"
     CDS             complement(21771..22286)
                     /locus_tag="ACFMWZ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC5934441.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPSYESDTFGLHCNVVTQPDGQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22293..22727)
                     /locus_tag="ACFMWZ_000433"
     CDS             complement(22293..22727)
                     /locus_tag="ACFMWZ_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934442.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEKAVRYTRRVTQVWCVFFIANGTL
                     AALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23008..23091)
                     /locus_tag="ACFMWZ_000434"
                     /pseudo
     CDS             complement(23008..23091)
                     /locus_tag="ACFMWZ_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(23094..23590)
                     /locus_tag="ACFMWZ_000435"
                     /pseudo
     CDS             complement(23094..23590)
                     /locus_tag="ACFMWZ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
BASE COUNT         5351 a         6742 c         6166 g         5331 t
ORIGIN      
        1 tcccataaaa aaacggagca gatacctgcc ccgtttttat ttaatccgaa attttaatct
       61 aaatttagaa ttttgcaccg gattggtttg ccatatagtc aacagccgct ttgacttcgt
      121 catcgctcaa acctgcattg ccgcctttgg caggcatcgc gttaaagcct tcaagggcgt
      181 gtttgtgcaa ggtttctttg ccttttttga tacgcggtgc ccaatcgtct tttttgccta
      241 tgccgggaat accgggaatc gaaccgccgt ggcacacctg acaggttgct tcgaagactt
      301 ttttaccgtc aacgccgacc gcaggggctg ccgcaccctt gtcttctgcc ttcgcttctg
      361 ccggagctgc actatcggca ggagcagaag ctgttcctga agcggcattg tcggcaggcg
      421 cagcctcatc aggattcggg aaagaaccgc cgcttttgtt cgccatgtaa gtaatcgccc
      481 gtttgagttc ctgatcggtc aggtctgccg caccgccttt ggcaggcatg gcgttaaagc
      541 cgttcagcgc gtgttggaac aaggtatcga agccttgcgc gatacgcggt gcccaatcgc
      601 cgttgtgttc cagtttcgga gcgttcggca cattgctgtc cgccgcgtgg cattggatac
      661 agattttgcc gaaaatctgt tcgccttggc gttcgccgac ggggatgccg tcgcccatcg
      721 tcaattgtcc gacaggctgg atacgggtct gcgttgctgc ttccgtagtg gcatcgacat
      781 cgccgaacga gccgctgccc gccagcttaa tcaggaaata aaggactgca ataacaataa
      841 cgataccgct cacaagggta aacagtgcag agccttgggc tttgttgtcg cggagttgtt
      901 tcatttggta ggcctcgccg tcaggttagg ttgtgctgta aattatagtt tggtgtgtta
      961 aacgcagtta acaatatttt gctggattat actgaattca cagggtcttt ccaatcgcta
     1021 tcattgaaaa tatgaaaaaa tttgccaacg gtatctgtat aaaacaaata atcctttgaa
     1081 aataatggtt tatcctcaag aaaactctcc ttatgccgcc atacgccgcc tgccggcgca
     1141 agataacctt tgccaatttg cagaatttac gttaacctta cgttttacgc acccatagct
     1201 cagttggaag agtgtcagtt tccgaagctg gaggtcacag gttcgatccc tgttgggtgc
     1261 gccaattata aagaggccgt ctgaaagata aatatttttc agacggtctt ttgacttact
     1321 tcaaactctt atttcaagac ttccgcaaat gcgcgggcaa catagtcggt attcgacgta
     1381 ttcagtccgg cgacgcacat cctgccggaa tccagcaggt aaacggcaaa ttcgtcgcgc
     1441 agcctgcgga cttgttccac gctcaatcct gtgtagccga acatgccgcg ctgtttgatg
     1501 aaataagtga aatcgcgatc ggggatttgc gcagttaaga catcataaag tttctgccgc
     1561 atcgcacgga tgcggtcgcg catcatataa acctcgtttt gccacaaggc gtaaagttcg
     1621 gggctgttca tcacgtcggc ggcgatatac gcgccgtgcg cgggcgggct ggagtagatg
     1681 cggcggacag tgaatttgag ctgtccgaac accaaatccg cttcttcctt attcgggcaa
     1741 accacgctca ggccgccgac gcgctcgccg tagagcgaca ggttttttga gaaggaattg
     1801 ctgacgaaca agggcaattc catttccacc gctttgcgga cggcgtaggc atcgctgtcc
     1861 aaatcgccgc cgaagccttg gtaggcaatg tccataaacg gaatcagttt gcgcgttttg
     1921 atgatgtgca acacttcgtc ccattgctgt tccgacatat ccacgccggt cgggttgtgg
     1981 cagcagggat ggaggatcag gacgctgttt tcgggcaggg tgttgaaaaa cgcggtcatt
     2041 tcgtcgaatt tcacgccaac agtggcaggg tcgtaatatg ggtaagtgcc gacctcgaaa
     2101 cctgcgcctt caaaaatgcc gcgatggttg tcccaagtcg ggtcgctgac gtaggcgcgc
     2161 gcttcgggaa accagcggtg caggaagtcc gccccgactt tgagtgcgcc cgagccgccc
     2221 aaggtttgta cggtagcgat gcgtccttgc gcgagcgcag ggttgtcttt gccgaacaat
     2281 aaatgctgca ccgcgctgcg gtaagtgtcc aagccttcca tcggcaggta gggcgacggc
     2341 gcaggcgtgg cggcgcgggc ggtttcggca cggcgcacgg attccaacac gggcattttg
     2401 ccttcgtcgt cgaaataaat gcctatgctc aaattgactt tttcagggcg cgggtcgttt
     2461 ttgaaggttt cgaccaaact caaaatcggg tcgcccggat agtattcgat gtgtcggtac
     2521 atagtcctta ccttttactt tttcaaagga ttttcttttt caacaataca ccactttcga
     2581 tatggtgcgt aaacgggaat tggtcgaaca gggcggcgcg ttcgaccgca tgggtttcca
     2641 ccaaggtgtc caaattggca cgcagcgttt cgggattgca ggaaatgtag atgatgttgt
     2701 caaactgcga caccagcttc aaagtttcct catcgatacc ggcacgcggc ggatcgacga
     2761 aaatagtgga aaatgcgtaa tccgtcaaag caataccgcc atccttaagg cgtttaaact
     2821 cacgttttcc ggtataggct tcggtaaatt cttcagcaga cagacgggcg attttgatgt
     2881 tgccgatgcg gttggcttcg atattccatt gcgccgcgct gatggaggtt ttggagattt
     2941 cggttgccaa aacacgttca aacttttcag ataaaggaag cgtaaagttg ccgtttccgc
     3001 aatacagttc gagcaaatcc ccatccaatc cttctgccgc accgcaggcc cattcaagca
     3061 ttttctgaca cacggcggca ttcggttggg taaaactgcc ttcaatttgc cgataacgga
     3121 aatcccggtt gccgaccttc aaagtttccg ttacatagtc ctgttttaag actattttct
     3181 gtcccctgct ccgcccgatg acggaaatat ccaactgttg ctgtaacgct tgcgccgcct
     3241 gcatccactc attatcaagc cttttgtggt aaatcatggt aaccagcatt tccccgctga
     3301 gcgtagacaa aaattcgacg gcataccaac ggtttctcag ttcggcagat tgggcggcaa
     3361 cggcaatcaa ttctggcatc aggcggttga cggcttcgga agccgcttca aaacggtcgc
     3421 agcgtatcat gcttgcgccg ctggctttct gccctttttc aaacatggca taaaacattt
     3481 ccccgccttc gtgccaaata cggaactcgg cacgcatacg gtaatgtttg tccggagatt
     3541 cgtacacttc ccactcagga acatccaaac ctgcaaaaag ggttttaagg tggtcttttt
     3601 tatcttgaag ctgctgcgtg tagtcgctca tatcgctatc ctgaaaaatc agaccggatt
     3661 ataagccgat tcactgcaaa acacgcaacc cctttaccgg catataaccc gttttcctcc
     3721 aacttttatg ctaaaatccg ccgtctccaa cataccgccg acagaaggaa aatatggcag
     3781 gcaacacttt cggacaactc ttcaccgtga ccacgttcgg cgaaagccac ggcgcaggtt
     3841 tgggctgcat catcgacggc tgcccgcccg gactggcatt aaccgaatca gacatccaag
     3901 ccgacctcga ccggcgcaaa cccggcacca gccgccacgt tacccaacgc cgcgaagccg
     3961 accaagtcga aatcctctcc ggcgtcttcg aaggcaaaac cactggcacg cccatcgccc
     4021 tcttaatccg caataccgac caacgcagca aagactatgg caacatcgcc caaagtttcc
     4081 gccccggcca cgccgactat acctactggc acaaatacgg cacgcgcgac taccggggcg
     4141 gcggtaggag ttccgcccgc gaaaccgcag cccgcgttgc cgccggagcc gttgccaaaa
     4201 aatggttgaa agaaaaattc ggcacggaaa tcaccgccta cgttacccaa gtcggcgaaa
     4261 aaaaaatccg gtttgaaggc tgcgaacaca tttcccaaaa tcccttcttc gccgccaacc
     4321 aaagccaaat tgccgagctg gaaaactata tggacagcgt gcgcaaatcg ttggattctg
     4381 tcggcgcgaa gctgcatatt gaagccgcca acgtccccgt cggcttgggc gaacccgttt
     4441 tcgaccgcct cgatgccgaa atcgcctacg cgatgatggg catcaacgcc gtcaaaggcg
     4501 tggaaatcgg cgcaggtttt gacagcgtaa cgcaacgcgg cagcgaacac ggcgacgaac
     4561 tcaccccgca aggcttcctg tccaaccact caggcggcat cctcggcggc atcagcaccg
     4621 gacaagacat ccacgtcaat atcgccatca aacccaccag ctccatcgcc accccgcgcc
     4681 gcagtatcga catcaacggc gaccccatcg aactcgccac gcacggcagg cacgacccct
     4741 gcgtcggctt gcgcgccgcg ccgattgccg aagctatgct cgccttggtt ttaatcgacc
     4801 acgccctgcg ccatcgtgcg caaaatgccg acgttcaggt taatacgccc gacattaccc
     4861 tttcaaacaa ataaaaattt agccaaaaca cagactttat aatagaatac caagcattgc
     4921 cgtgcagcct tgacgcacgg gtttgtttag aggaatacaa ccgaaatgac acaagaaacc
     4981 gctttgggcg cggcactgaa atccgccgtc caaactatga gcaaaaagaa acagaccgaa
     5041 atgatcgccg accatatcta cggcaaatac gatgtattca aacgatttaa accgttggca
     5101 ctcggtatcg accaagactt gattaccgcg ctgccccaat acgatcctac acttattgca
     5161 cgcgttctcg ccaaccactg ccgccgtccg cgctatctga aagccttggc gcgcggaggc
     5221 aaacgtttcg atttgaacaa ccgtttcaaa ggcgaagtta cccccgaaga acaggcgatc
     5281 gcgcaaaacc atcctttcgt gcagcaggct ttgcaacagc agtccgccca agctgccgcc
     5341 gaagcagccg gatcttccgc agcagaataa atccccaaac gaaatgccgt ctgaaaaccg
     5401 atttggtttc agacggcatt ttttcgtatg cggcaatcac ggttcaaata tccaattccg
     5461 ccgtatcgcc ttcgcgttcc atccaagcgc ggcgggcggc ggcttcgcct ttgcccatca
     5521 gtttgacgaa gatgtcgcgt gtttcgtcat ctgcgccttc tgggatttgt acctgcaaca
     5581 gacggcgggt gtcggggtgc atggtggtgt ctttgagttg gtcggggttc atctcgccca
     5641 agcctttaaa acggctgatg gaataagcag tttctttaaa gccttctttt tgtagtcgct
     5701 ccaaaatgct gtcgagttcg ttttggtcga gggcgtagaa tttgcgggcc ggtttgtttt
     5761 tgccttgtgc attgacatcg acgcggaaca gcggcggctg ggcgacgtag atgtgtccat
     5821 cggcgaccag tttcgggaag tggcggtaga acaaggtcag cagcaaaact tgaatatgcg
     5881 agccgtccac gtcggcatcg gacaggatgg cgattttgcc gtagcgcagg ccgcttaaat
     5941 cgggatggtc gttaataccg tgcggatcga cgccgatggc gacggaaatg tcgtggattt
     6001 cggcgttgcc gaagagttgg tcggggtgga cttcaaagct gttgagcact ttgccgcgca
     6061 ggggcaggat ggcttgggtg gctttgtcgc gggcgagttt ggctgagccg ccggcggaat
     6121 cgccttcgac gaggaagagt tcgttttcgc ggatgtcttc gctttcgcag tcggtcagtt
     6181 tgccgggcag gacggcgacg ccgctgcctt ttttcttttc gattttctta accgaacgca
     6241 tccgcgcctg cgcctgacgg atggcgagtt cggcgatttt tttaccgaag tccacgtttt
     6301 ggttcagcca caattccaaa gggtcgcccg atacggcggc aacgagtttc aacgcgtcgc
     6361 ggttggtcag cttgtctttg gtttgacctt ggaactgcgg gtcgaggacg cgggcggaga
     6421 ggacgaaggc ggttttaccg aacacgtcgt cactttgtac tttcacgccg cgcggtagga
     6481 ggttgtgcag attgatgaaa ttgttgacgg cgttgaacac ggcttgtttc aaacctgctt
     6541 cgtgcgtgcc gcccagcggg gtggggatga ggttgacgta gctttcgttg gcgcacgagc
     6601 cttcttccag ccaggtcagg gcaaacgccg ctccttcgcc gatgctgaaa tcgccgttgt
     6661 ggtcgtctga aatgtagttt tcgcaagaga agataggcac ggcttcctgc gcgtcggcaa
     6721 tcaggtcggt cagatagctt ttcagaccgt cggggtaatg ccaggtttgg gtgtgcgctt
     6781 cgtcttcgcc tttgaccgga cgggtcaggg aaacgcgcac gcccggcagc agcacggctt
     6841 tggcgcgcag caggcgttcg agttcgggaa tgctgtaatt cgggctttca aaatatttgc
     6901 cgtccggcca cacgcgcact tcggtgccgc tgtctttgac ggcgcatttg cccacttgtg
     6961 ccaacggttc gaccacgtcg ccgccggcaa acacgatacg gtggattttg ccttcgcgtt
     7021 tgaccgttac ctcaaggcgg gtggaaaggg cgttggtcac ggatacgccc acgccgtgca
     7081 ggccgcctga aaaggcatac gcgctgccgc cgtctttttt gttgaacttg ccgcctgcgt
     7141 gcagacgggt gaacacgagt tcgactacgg gtacgccttc ttcagggtgc agaccgacgg
     7201 gaataccgcg gccgttgtcg cgtacggaaa gcgaaccgtc ttcatgaatt tgcacgtcga
     7261 ttgccgtagc gaaaccgccc aacgcctcgt ccgccgcgtt atcaatcact tcctgacaga
     7321 tatgggtcgg gctgtcggtg cgggtgtaca taccgggacg ttctttgacc ggttccaaac
     7381 ctttgaggac ggtgatgctg gattcgctgt attggttgtt tttagccata ggaataatct
     7441 gaaagtaaga aaaacaacgc tttcagacgg cctgaaagcg ttgcgttccg ttgttttacc
     7501 ggttgccgga aggttggcgg gcggcaaagt cttcataact ttccataccg cgcaggaagc
     7561 gggaagaaag ttctttcaac gcccccaaat aaacgtcgcg tttgaaatca atcacttcgt
     7621 caacgggcgc ccaatattgg tgccaacgcc agccgtcaaa ttcggggtgg cgggtggcgc
     7681 gcaggttgac atcgcaatcc cggccggtca ggcgcaggag ataccaaatc tgcttctgtc
     7741 cgcgatagga tccgcgccat tcgcggcgca cccagttgtt cggcacgtcg taacgcagcc
     7801 agtcgcgcgt ccgcccgacg attttgacgt gttgcggcag aagtccgact tcttcgtaaa
     7861 gctcgcggta catcgcggtt tcggggcttt cgcccggctt gatgccgcct tgcggaaact
     7921 gccaagaatg ttcgcgcacg cgcttgcccc aaaagacttc gttacgttcg ttaatcagga
     7981 taataccgac attggggcga tagccttccc tgtccaacac ggtgtcgccc tccgttaaat
     8041 tcaatcgtac gattttccca caaatcgggc ggttttgaca aatcagacgg catggttcaa
     8101 gcatgtcgaa acacagacgg actcgggaaa atatcttaaa tttggtttac aataatgtgt
     8161 ttcaaattat tcgggaatca gaccatgtta gatatccaat tgctccgcag caacaccgcc
     8221 gctgtggcgg aacgcttggc ggcgcgcggt tacgagtttg atgccgcgcg tttcaatgcg
     8281 ctggaagaac aacgcaaagc cgtccaagtg aaaaccgaag aactacaagc ctcgcgtaac
     8341 agcatttcca agcaaatcgg cgcactgaaa ggtcagggca aacacgaaga ggcgcaggcg
     8401 gccatggatc aagtcgccca aatcaaaacc gatttggaac aggctgccgc cgatttggat
     8461 gccgttcaaa aagaattgga cgcatggttg ttgagcatac ctaacctgcc gcacgaaagc
     8521 gtacctgccg gtaaagacga aaccgaaaac gtcgaagtcc gcaaagtcgg caccccgcgc
     8581 gaatttgact ttgaaatcaa agaccatgtc gatttgggcg aacctttggg tttggatttt
     8641 gaaggcggtg caaaactctc cggcgcacga tttaccgtga tgcgcggaca aatcgcccgt
     8701 ctgcaccgcg ccttggcaca gttcatgctg gatacgcaca cgctgcaaca cggctacacc
     8761 gagcattaca cgccttatat cgttgacgat acgacgctgc aaggtacggg ccaactacca
     8821 aaatttgcgg aagatctgtt ccacgttacc cgtggcggcg acgaaaccaa aaccacccaa
     8881 tacctgattc cgacagccga agttaccctg accaataccg ttgccgacag cattatcccg
     8941 tccgaacaac tgccgctgaa gctgaccgcg cattcgccct gtttccgcag cgaggcgggt
     9001 tcgtacggca aagacacgcg cggtctgatt cgccagcacc agttcgacaa agtggaaatg
     9061 gttcaaatcg ttcatcccga aaaatcatac gaaacgctgg aagaaatggt cggccatgcc
     9121 gaaaacatcc tgaaggcttt ggaactgccc taccgcgtga ttaccctgtg taccggcgac
     9181 atgggcttcg gcgcggcaaa aacgtatgac ttggaagttt gggtgccggc gcaaaatacc
     9241 taccgcgaaa tctcaagctg ctccaactgc gaagatttcc aagcccgtcg catgaaggcg
     9301 cgtttcaaag acgaaaacgg caaaaaccgc ttggtacata ctttgaacgg ctccggtttg
     9361 gcagtcggca gaaccttggt cgccgtgttg gaaaaccatc aaaacgccga cggcagcatc
     9421 aacatccctg ccgcactgca accgtatatg ggcggcgttg ccaagttgga agtgaaataa
     9481 gccttaaccc tgaacataaa tgccgtctga aacttggttc agacggcatt tcctttaaac
     9541 ttttaaaaca cgtcagccgt cggcacgaac cgcattgccg caatcgccgg tctgtccgac
     9601 ctcgcggata ttggacagcg taacttccga aatattaccc aacgcctctt ccgtcaaaaa
     9661 tgcctgatgg ccggtaaaca gcacattatg acaagacgac aggcggcgga acacgtcgtc
     9721 ggtaatcaca tcgttggatt tgtcttcaaa aaacagctcg cgctcgttct cgtacacatc
     9781 catgcccaat gcgccgattt tccggcgttt caacgcctca atcgcggcgg cactgtcaat
     9841 cagcccgccc cggctggtgt tgataatcat cacgccgtct ttcattttgt cgaacgccgc
     9901 ttcgttcagc atatagtggt tttccggcgt ggaggggcaa tgcagcgtga tgatgtccga
     9961 ctgggcatac agctcgtcca aatccacata ttttccgccg agtttttcca cttcggggtt
    10021 gcaaaacgga tcgtaagcca gcaggttcat accgaaaccc tttaaaatcc gcatcgttgc
    10081 gataccgatt ttccccgtgc cgataacgcc cgccgttttg ccgtacatat tgaaaccggt
    10141 cagaccttcc agcgaaaaat tcgcatcgcg ggtacgctga taggctttgt ggatacggcg
    10201 gttcaacgtc agcatcagac cgaccgtatg ttccgcaacc gattcgggcg aataggcagg
    10261 cacgcgcacg actttcaagc ccaactcttc agccgccttt aaatccacat tattgaagcc
    10321 ggcacaacgc aacgccacag ttttcacgcc aatttgcgcc aatttttcca acacgggccg
    10381 gctgccgtcg tcgttcacaa aaatacagac cgcctccgca ccttccgcca ttttcgccgt
    10441 tttcgcatcc agcataaaat caaaaaactc cagctcgaag ccgaaatgcc ggttggcgcg
    10501 ggtaaaatgt tcgcggtcat agcttttcgt accgtaaatc gcaatcttca tcaatatgtc
    10561 cagttgtcgt ctatggttga gaaacggcat aatacactga attcaaacaa atcagtagaa
    10621 tatggtggat taaaattgat tgcatgcacg gcatttccat ttcaaaacac aaaactcaat
    10681 cgcccattgc cgccagaagc tccgcctgat gctcggcaat cagggcatcg gtgatttctg
    10741 ccaaatcgcc gtccatcaca aaatccagct tgtgcagggt gaggttgatg cggtggtctg
    10801 ttaccctgcc ttggggatag ttgtaggtgc ggatgcgttc gctgcggtct ccgctgccga
    10861 tgagggattt gcgttcggcg gcttctttgg cttgggcttc gcgtttttgc gcgtcgttca
    10921 ggcgggcggc gaggactttc atcgcctgcg ctttgttggc gtgttggctg cgtccgtctt
    10981 ggcattcgac caccatgcct gtgggcaggt gggtgatgcg gacggcggaa tcggttttat
    11041 tgatgtgctg cccgcccgcg ccggatgcgc gaaaggtgtc gatgcgaagg tcgacggggt
    11101 tcaactcgat gtcttccagt tcgtccgctt cgggcatgac ggcaacggtg caggcggagg
    11161 tgtggatgcg gccttggctt tcggtggcgg ggacgcgctg cacgcggtgt ccgcccgatt
    11221 caaatttcaa acggctgtac gcgcctaagc cgataatacg ggcgataact tctttatagc
    11281 cgcccaactc gctttcgttg gcggacacga tttcaacctg ccagcggttg cgttcggcgt
    11341 agcggctgta catacgcagc aaatcgccgg caaacagcgc ggcttcgtcg ccgcccgttc
    11401 cggcgcgtat ttcgatgaag atgtttttgt cgtcgtcggt atctttgggc agcagcagtt
    11461 tttgcaattc ggtatcgagt gcgccgattt tggctttcgc cgcttcgatt tcttcggcgg
    11521 caaagtcttt catttcgggg tcggacaaca tttcttcggc atccgccaaa tcgctttgcg
    11581 ccagttgata gttttggaat acttcgacga cgggggtcag ttcggcgtgt tcgcgcgtga
    11641 gcttgcggta gttgtccata tctgccgtgg cttcgggttg ccctaaaagg tgggtaactt
    11701 cttccaaccg gtcgccgagt tgttgcagtt tttctaagat agacggcttc ataattcttc
    11761 cataacaaac gccgcctgaa tttcagacgg catcaaaacg cggtcattat aatatatttt
    11821 ctgaatattc aaaaagataa tctagggacg gagcaggctg tcggcattgc gttccgttat
    11881 cgcggcgatg tccgccacgc cgacaccgcg tatctgcgcg gcaatttcgg caatccaaag
    11941 aatattggca ggcgtattaa tctctttttt cagcataaac gggctgtcgg tttccaatac
    12001 gaaatccccg tcgttcaagg ctttaagcgt atcgcgcact ttacgcgcgt tcggattgag
    12061 caacaacgaa ccgatgccga ttttgaaacc cagtttcgtc aacacgcgcg cttcttccgc
    12121 gctgcctgag aaggcgtgaa cgatgccgcc ttgggcaaag cctgtctgtt tgacggcggc
    12181 ggcgatgtcg gcggtggctt tgagattatg gataatcacg cggcggcgca gggtttgcgc
    12241 gatggcaagc tggcggacga aaacttggat ttgccgttcg cgctgcggcg gcgtttgggt
    12301 tttgtcgtaa aaatccaagc cgatttcgcc gacccatgcc tgcggataac gtgccaacat
    12361 cgcttccagg cggacgcaat cacgctcggc aatgccgtct gaaaaccaag gatggatgcc
    12421 gagcgcaata cggatttgac cgtgttcgga cggcatttcc gacagttccg ccacgctttg
    12481 ccaatcctgc ggacaggtgg cgggaacgat aaaccgcttc accccaactt tccgcgcggc
    12541 ggtcaggatg tgcggcaggt tttcgcgcag ggcggcatcg gcaaggtggc agtgggtgtc
    12601 ggtgaagttc atttcgattc cgcaatgcgg caggttcaca taaaaataag gcagggcagc
    12661 aagggatgcc gtgtacgggc ggcgcataag gcgcggtcaa cgccccgtca ttgccatttc
    12721 aatccgctat aacttcagtc ttgccaactc tttttccacc tcttccttac cccagccttg
    12781 cgatacggcg agggtcatga gtgcggtggc ggtttcgagg ttgcatttgc cgccgttgat
    12841 gatgcctgag ttgcggaacg cgttgccttg cgcgtaaacg gcagcggttc tgccttgtcg
    12901 gacttggctg atgttgagca gcagcctgcc ttgctgcgta aagtcttgca cggcgcggat
    12961 gaaaccttcg tctgcgggcg tgttgccgtg tccgtaactt tgcaggataa gggcgtgggc
    13021 gggaagctgt ccgagtccgt cggcaagttc ttggacggca aagccgggaa taagcgtgcg
    13081 gacggcgatt tttgcctgcg ggtcaggata gcggattttg aggccgtctg aaacggccgc
    13141 cgcatcttgg gacggaatac gaagattgta ccagccttga gtttcatccc attcggcgat
    13201 gacaccgaaa tgcggattgt cgaagcctgc ggcggtttcg gtgctgactt tgctgctgcc
    13261 gacggcggga taaagtttgc cgtcaaaggc aataagcgtc tgcttcaatc cgagtgggaa
    13321 tgcggcaacg gcagtcgcca gattgcgcgg cgcgtcgctg cccacggcgt tgtacggcca
    13381 ttgggaaccg gtcaggacaa tcggtttgac caaaccctgc agcgcgaggg cgaacagatt
    13441 ggcggtatag gcgagcgtgt ccgtaccgtg cagcaccaaa acgccgtcgt actgcggcag
    13501 cgcggcggca atgatgtcca gccaagcgca ccagttttgc agttggacgg cggaagaatc
    13561 aatcagcgtc ccacaaacgt gccaatcgaa atccatgccg tttgaaaacg gcgcgagtgc
    13621 ctgcccgacc agcgcggtat cggggcgcag gccttcgctg ctttgcgtca tgccgatggt
    13681 gccgccggta taaaggacaa aaattttctg tttcatggct catcctgaaa atgcaaagac
    13741 cgcctgaaac gcgaaatgcg tccggacggt cgaaaatctg atttactgct gcttctgtgc
    13801 cgcttttttc gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca attgtgcgcg
    13861 gtaaagctga taatgtcggc gtttgcgcca ccagaaccaa gccagcgccg ccgccagcac
    13921 gcccaatgcg atgaagatgc cggattgcag gctgtgcatt ttcgccatca gccaatcgat
    13981 gttgtgcgcg ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca gcgcggcaag
    14041 cccgtccata atcagaaagc gcagatacga tactttgcgg ctgatgccgg cggtaacgaa
    14101 aacggcagtc cgcaaacccg gcaggaagcg ggcgacaaac agaacccagt tgccgtattt
    14161 gtcgaatttt tcctgaacct gtgcgtaacg tttcggcgtc atgatgcgcg caatcggttt
    14221 gaacttgagg attttctgcc cccaaattcg tccggcggcg aacatgatgc cgtcgcccgc
    14281 caacacgccg agcataccga ctgcaaacat aatatgcgga ttggtataac ccatacccga
    14341 aatcacgccg cctgttacca aggtcaaatc ctcgggaatc ggcacgccga aaccgcagat
    14401 gaccaaaacg aaaaacacgg ccgcgtagcc gtattcgaca aaaaaggctt ccaaaagggc
    14461 aaacatagcg gatattccat tgtcggagat aaaaagtcag aacaagccga aacattttct
    14521 gcgcgaaacg gcattctatc aaagattatg ccgtctgaaa gcggcaaggg gcagattcgc
    14581 gccctctgcc ggacgttcag acggcattta aacaggaaaa ccgccgctgt tgaaacggtt
    14641 ttgcctgatt ttgcctaaag gctgccgatg gcggaagcaa tacgttccgc accgtcgcgc
    14701 gcccaatccg cctgccgcgc ctcaaccatc actcgcacga cgggttcggt tcccgaagcg
    14761 cgcaacacga cacgcccttt gccttcgagt tctttttcca cttccgccaa cacgtctttt
    14821 gaggcttcct gccatttctg acctttttgg atgcgtacgt taatcatagt ttgcggatac
    14881 ggctgccagt cggcgcaaac ggtggcgagg tcttggttca ggatttgcag cgccgccaaa
    14941 acttgcagcg cggaaataat gccgtcgccg gtgttgtgtt tgtccatgca caaaatatgg
    15001 ccgctggctt cgccgccgat gagccagccg cgttggttca gctgttccaa cacatagcgg
    15061 tcgccgactt tggcgcggca gaaatcgacg ccctgctctt tcagggcgat ttccatcgcc
    15121 atattggtca tgaccgtgcc gaccacgccg ccgatgttga taccttctcg ggcgcgggct
    15181 ttggcaatga cgtaaatcag gctgtcgccg tcgtaaacct taccgttttt atcgaccatc
    15241 atcaggcggt cgccgtcgcc gtctaaggcg atgccgtagt cggcttcatg ctgtaaaacg
    15301 gctgcctgga gtgtcttggt ataagtcgca ccgcattttt cgttgatgtt gtagccgttg
    15361 ggttcgtcgc cgatgctgac gacctgtgca cccagttcgt gaaacacctt gggggcgaca
    15421 ccgtaccccg cgccgttggc ggtatcgata accaagttca aaccccgaag gtcggaatgg
    15481 ctgggaaagg tggatttgca aaattcgata tagcggtcgt ccgcaccact gatgcggcgt
    15541 gcgcgcccga ggcgtgcaga aggctgggtt ttcatttctt cgtcgatttt ggcttcgatt
    15601 tccaactcga tttcatcgga aagcttcacg ccgccctcgg caaagaattt gatgccgttg
    15661 tcaaaataag cattgtgcga cgcggaaatc atcacaccgg cggacaaacg cagcgcacgg
    15721 gtcagataag ccacgccggg cgtgggcagc ggtccggtct gtaccacatt cacacccgcc
    15781 gccgtaaaac cggccaccaa agcggcttcc agcatataac cggaaatacg cgtatccttg
    15841 ccgattaaaa cggtcggttt ctggtcagtg tcgtgctgaa ccaaaacctg acccgccgca
    15901 tagccgagtt tcaatacaaa atcgggcgta atcggaaact gcccgacttc gccgcgcacg
    15961 ccgtccgtgc cgaaatattt ttttgccatg tgttgctccg agaatgtgaa ccgttgtccg
    16021 agattataca gtcagtttgt gccttgctgt ctgcaccgtt gatgccgtct gaagccgccc
    16081 cgcccttttc agacggcatg aagtatgcga accgctgttt acagattgat gcccaacgct
    16141 tcccatgcct tcaacgcatc cgccgtcgcc ttcacatcat gcacccgcac gatttgcgcg
    16201 ccgcgcgcca cggaagccag tgccgccgcc acgctgccgt gtacgcgcgc cgccgcgtcg
    16261 gtttcgccgg tcagctcgcc tatcatgcgt ttgcgcgata cgccgataag cagcggaaag
    16321 cctgtttctg ccatcaattc gggtaaatgc cgcatcagtg cgatattgtg ttgtaaggtt
    16381 ttgccgaaac cgaaaccggg gtcgagtgtg atgcgttgcg gggcgatgcc tgccgcgatg
    16441 cattccgccg cccgcgcttt caaataccgc gccacttcac caacaacatc ctgatatttc
    16501 ggattaatct gcatggtttc aggcaaaccc tgcatatgca tcaggcaaat acccatgtcc
    16561 gtctggcgcg ccagcaattc gaccgcgcct tcgtcggtca acgccgccac atcgttaata
    16621 atatcgatgc cgccgagtgc caacgctttt tccataacca ccgtgcggcg cgtgtccaaa
    16681 ctgacgggaa cgccccatcc cgcgacttct accaatacag gctcaacccg cgcccattct
    16741 tcttcggacg aaacataatc cgcacccggc cgcgtcgatt cgccgccgat gtcgagaatg
    16801 tccgcaccct ctttcaaaag ccgttcggcg tgcgccaaag ctgtttgggc attttgcgaa
    16861 tacgcgccgc cgtcggaaaa agaatcgggc gtgagattca cgatacccat gattttcggt
    16921 ttgtccaaac cgatttcaaa ccgtcctgcc tgccaaacgt gtcgtgccat ctgaactcct
    16981 cccaaaataa aaaacagatt atatgccgtc tgaaaccgtc ttgtgcgctt cagacggcac
    17041 cgctattcgg gcggcagacg gcatgttgtc cgaatgtctg ctccgccttt gaatctgccg
    17101 gtatgcctgc tatccgcccg acttttcaaa acaggttccg acgattccgc acgcgcctgc
    17161 cgcctttgcc aagccgtaca ggatttcctg cggcatatcg cggttccata atcccgtaat
    17221 attcgcaatc acgggcagat ggctgatttg gcggactttc acgatggatt cgacatccaa
    17281 acggtaggga tggtctttgg tatggctcaa cacgcccgac tcgcccagaa tcaggtggcg
    17341 gttgccgcgc gagacgacat attctgcggc attcaaccaa tcttcggcag aatggtgctt
    17401 gtctttacac agcaccaagg gaatgttcag gcttccggct tcattcaata cggcaagatc
    17461 ggacatcagc ccgccgccca aatacaggat gtccgccccc gcattcaaag ccgcttcgac
    17521 atggcggacg ttgcggacgc gcaccaatac gggtttccct gcgccatgcg ccgatgcggt
    17581 ctgttccacc aaccgtctgc accgtccccg cccttcatcc gcacttgaag tgtcgtataa
    17641 gtttgccgaa gtgaaaaacg gatccagaaa cactgcatcc gcattgcgcc atactgacgg
    17701 ttctgcggcg atacggacgg tttccccgcc gccgaaagcc acgcctttgg cggcaacgcg
    17761 gctgtcttcc gcccgatttt cccgactgac ggttttccat gtatccaaaa tgcggacggc
    17821 tttctcgacc tccggcagcg tctgcacctc cctgacgctc aaaaccctat cgtcgccgat
    17881 tgcgccgatg acagtacgct cgtcgccgtg agaaatgtgt tctcgcagac ctctgctgcg
    17941 gataaaggcg acaacgccgg caatgtccgc ttcggcggca cgcctgctca tgacaataat
    18001 catatttcct cctgacacaa gaaacggcct gcccaaaata ggatttttgc aagccgtgtt
    18061 atactgtggc gtgttttaca gattgttcgg gctatggatt tattatcggt tttccacaaa
    18121 taccgtctga aatatgcggt ggccgtgctg acgatactgc ttttggcggc agtcgggctg
    18181 cacgcttccg tatatcgcac cttcacgcct gaaaacatcc gcagccgcct ccaacaaagc
    18241 attgcacaca cacaccggaa aatctcgttt gatgcggaca ttcagcgcag gctcctgccc
    18301 cggccgaccg tcatcctgaa aaacctgacc attaccgaac ccggcggcga ccagactgcc
    18361 gtttccgtcc aagaaaccaa aatcggattg agctggaaaa acctgtggtc ggatcagata
    18421 cagattgaaa aatgggtggt ttcgagtgcg gaacttgccc tgacgcgtga cgggaaaggt
    18481 gtttggaaca tccaagacct gatcgacagc caaaaacgcc aagcctcagt caaccgcatt
    18541 atcgtcgaaa acagcaccgt ccgcctcaat ttcctgcagg aacagcttat cctgaaggaa
    18601 atcaacctca acctgcaatc ccccgattcg tcggggcagc cgtttgaaag ttcgggcata
    18661 ctggtttggg gaaagctgtc cgtcccgtgg aaaagcaggg ggctgttcct ttcagacggc
    18721 atcggcacgc ccaaaatctc accgttccat tttgaagctt ccacttcgct ggacggacac
    18781 ggcatcacca tttccaccac cggcagccct tctgtccgtt tcaacgccgg cggagcggat
    18841 gccgccggcc tcggcctgcg tgcagacact tccttccgca acctccacct gaccgcccaa
    18901 atccctacgc tggcactcag gaacaacagc attaaaattg aaaccgtcaa cggcgcattt
    18961 accatcggcg gcgaatatgc ccgatgggac ggttcgttca aactcgacaa agccaacctg
    19021 cactccggca tcgccaacat cggcaacgcc gaaatctccg gcagcttcaa aacaccgcgc
    19081 caccagacca acttctccct caattcgccg ctcgtatgga cggaaaacaa agggctggac
    19141 gcgccgcgcc tgtatgtatc gacccttcag gataccgtca accgcctgcc gcaaccccgt
    19201 ttcatcagcc ggctcgacgg ttcgctgtcc gtaccgaatc tgcaaaattg gaatgccgaa
    19261 ttaaacggca cattcgaccg ccaaaccgtt gccgcgaaat tcagatacac acatgaagac
    19321 gcaccgcatc tggaagccgc cgtcgcactg caaaaattga acctgacccc ctatcttgac
    19381 gacgtgcggc aacaaaacgg caaaatattt cccgacaccc tcgccaagct gtccggcgac
    19441 atcgaggcgc acctgaaaat cggaaaagtc caacttcccg gcctgcaact ggacgatatg
    19501 gaaacctacc tccacgccga caaagaccat atcgcgctca gccgtttcaa gtcagggctt
    19561 tacggcggcc ataccgaagg cggcatcagc atcgccaaca cccgtcccgc cacttaccgc
    19621 ctgcaacaga atgcaagcaa catccaaatc caaccgctgc tgcaagacct gttcggcttc
    19681 cacagcttca gcggcaacgg cgacgcggtc atcgacctga ccgcgggcgg cgaaacccga
    19741 aaagagctta tccgctcgct tcagggcagc ctgtcgctaa atatttccaa cggtgcatgg
    19801 cacggtatcg acatggacaa tatcctgaaa aacggcattt cgggcaaaac tgccgacaat
    19861 gccgcaccca gcacaccctt ccaccgattc acgctcaaca gcgaaatttc agacggcatc
    19921 agccgccaca tcgataccga actcttctcc gacagcctct atgttaccag caacggctat
    19981 accaatctgg atacgcagga attgtctgaa gatgtcctta tccgcaacgc cgtccatccg
    20041 aaaaacaaac cgattcccct gaaaatcacc ggtacggtgg acaagccgtc cattaccgtc
    20101 gattacggca ggctgaccgg cggcatcaat tcgcgcaaag agaaacagaa aatcctcgaa
    20161 gacaccctgc tggaacaatg gcagtggctc aaacctaaag aaccgtaaac atcctgcgta
    20221 caaaaatgcc gtctgaaacg ccctcgcgct tcagacggca ttgccgtaaa acctacaagc
    20281 ccaattcccc ccaaatccca tcaatcttcg ccgtaaccgc agggtctttt ttgatgacgc
    20341 gcccccattc gcggtcggtt tctcccggcc atttgttggt cgcatccaaa cccattttgc
    20401 cgccgagtcc gctgacgggg ctggcgaagt cgaggtagtc gatgggcgtg ttgtccacca
    20461 aaacggtgtc gcgcacgggg tccatgcgcg tggttaccgc ccagatgact tctttccagt
    20521 cgcgcacgtt cacatcgtca tccaccacga tgatgaattt ggtatacata aactggcgca
    20581 ggaacgacca gcagcccatc atcacgcgct tggcgtgtcc ggcgtactgt tttttcatgc
    20641 tcaccaccgc catgcggtag gagcagcctt cgggcggcag gtagaaatcg gtgatttcgg
    20701 ggaactgctt ttgcaaaagc ggtacgaaca cttcgttcaa cgccacgccc aaaacggcag
    20761 gctcatcagg cggtttgccc gtgtaggtcg aatggtaaat cgggttttcg cgcatggtaa
    20821 tgcgttcgac cgtaaacaca gggaaataat cctgctcgtt gtaatagccc gtgtggtcgc
    20881 cgtatgggcc ttccaacgcg gtttcgttcg gatgaatgac gccttccagt acgatttcgg
    20941 cacgtgcggg cacttgcaaa tcgttgccga tacatttcac cagttccgtc cgcgaaccgc
    21001 gcagcagtcc ggcaaactgg tattcgctca aggtatcggg aacaggcgtt accgcgccca
    21061 aaatggtggc ggggtcgcag ccgagcacaa cggagacggg atacgacgta tcaggattga
    21121 gtttgcggaa ttcctgataa tccaacgcgc cgccgcgatg cgacagccag cgcataatca
    21181 gcttgttttt gccgatgagt tgttggcggt aaatgccgag attttggcgt tttttgtgcg
    21241 gcccgcgcgt tacggtcaag ccccatgtta ccagcggcgc aacgtcttcc ggccagcaat
    21301 gctgaatcgg aagtttatac aaatcaacgt tttcgccttc ccacacgatt tcctgacacg
    21361 gcgcgttttt caccacgttc ggcgccatgc tccaaatgtc tttcagtagc ggcagtttgg
    21421 aaaacgcatc tttgatgcct ttgggcggtt cgggttcttt caaatacgcc agcgtctgcc
    21481 cgatttcacg cagcttggac acgctgtccg cgcccatgcc catcgccaca cgttcgggcg
    21541 tgccgaacag gtttgccaac acgggataat cgtagcgcgt accgtcgggc ttaatcgggt
    21601 tttcaaacaa caacgccgac ccttcggcac gcagcacgcg gtcggcgatt tcggtcattt
    21661 ccaaatgcgg ggaaacgggg tgggcgacgc gcttgagttt gccttgctgc tcgagcatga
    21721 cgatgaagtc gcgcaggtct ttgtatttca tatttatcct ttttgtcctt ttatcctgaa
    21781 caatccgatt cggataccgc ccctatcctt gcctgtgctt cggcatattc tatgccgtga
    21841 taaaagtcgc gtaccagcgg atgtccgccg ctttgatgaa gttgcagcaa agggcgttga
    21901 ccatcgggtt gggtaacgac attgcaatgc aaaccgaagg tgtcggattc ataagagggc
    21961 agccggttgc agatcatgcc gaaataaacg gcgttttccg gattgtcgtg aaagtggctt
    22021 tgatagtcgt taaaactctt ttcgctgacg gacacccaca cgccatattc cagcgtttcc
    22081 tcatgcccga taatcggaat cggcagcacc gcgcggataa agcggtcggt ttggtcggga
    22141 tagcggatga tgcagaaatc agaatcgcat tctgcttgat aagcaatgcg ttcttcttca
    22201 ctgagttgat tatagggatc gggggcggta aagccgattg cgggcatatc ttcatgtttt
    22261 tcgccgcagg aagtgcaagt gtacataagg tttcagactt tcaaaacgag tttgcggtaa
    22321 agccattcgc cggcaaacag catccccatc aatacatagg caatcacgcc tgtataaacc
    22381 gcccaccaat catatcgccc caaccgggcc aacaaagcgg caagcgtccc gttggcaata
    22441 aagaatacgc accaaacctg cgttacccgg cgggtatagc gcacagcttt ttcaggcagg
    22501 tcgggctgtt gcagccgcgc gagcctttct atgaccgtct gtccggcaaa caggctgccg
    22561 ccgaacaccg ccaacatcat cagattgacg aggacgggat accaatacat cgaatcatgc
    22621 cgcccgaaca ccaatactgc ggcaaaaaac agtgtaataa acaaagccgc ataacgctgt
    22681 tggggcagtt tggcagtcag ggcgcgcagc agccacaacc cgcacatcgc cgccgccagc
    22741 cagacaaacc agcccgcctc cctgccgtaa tatagtggat taacaaaaac cagtacagcg
    22801 ttgcctcgcc ttgccgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
    22861 actataccac aaagcgggat aggcaatgct taatacggtc agaaaaatat gtccgaaaaa
    22921 accgggtttc attttgaatc cgcacaaatg ttttcagacg gcatccgata aaaacatgcc
    22981 gtctgaaaaa taattagaaa tacccgatta gcccgcctga atcttcaata ccgcctgtac
    23041 cacgtcgttg acggtgcgga cattgcggaa atcttcagcc tgcagcttgc gggctgccac
    23101 agccattcac gattgcgata tacccggcgg cattcccgac tgccgttcag cagaaagcgc
    23161 acccattcca aaccgctcca atatgcctcg ggcagcatac cggcttcaga cggcatatcg
    23221 tccgaagcag ataaagtcag gctgtaacgc gtccctttgg tcagaaccat cgccaaagca
    23281 taagcaaacg gcgcgcgcgt tgccgatacg gcatatcctt ccggcagcgg atcgtccgcc
    23341 gccaaaacca aaaccgaccc gcatccctct tccaacagtg atgccgcttc cgccaatgcc
    23401 gtttccacac cgtcggcaca cacggccaat gccgtctgct cgctcatatc ccgcctcaat
    23461 atcgaccatt accccgccgt tgcattgtgc accgacaaac cgaacgaagt cggcgacacg
    23521 atttgcgatt tcaacagttc caaccacaaa tcgaaactgc gcgccatttc cccgtcgtgc
    23581 gaggcataaa
//
LOCUS       DAXHKX010000012        22444 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHKX010000012 DAXHKX010000000
VERSION     DAXHKX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.012"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..606
                     /locus_tag="ACFMWZ_000436"
     CDS             <1..606
                     /locus_tag="ACFMWZ_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC5934443.1"
                     /translation="YKIGISDVSNFEGRMRHLENNGYANVAGLERILAVKTDNYKEKE
                     NLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQE
                     GNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGKSGTRLT
                     DEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            643..1404
                     /locus_tag="ACFMWZ_000437"
     CDS             643..1404
                     /locus_tag="ACFMWZ_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC5934444.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHDAIAGNALMVKAMLE
                     YGANPASTTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            1459..1800
                     /gene="fdx"
                     /locus_tag="ACFMWZ_000438"
     CDS             1459..1800
                     /gene="fdx"
                     /locus_tag="ACFMWZ_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC5934445.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2087..2674)
                     /locus_tag="ACFMWZ_000439"
     CDS             complement(2087..2674)
                     /locus_tag="ACFMWZ_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC5934446.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDPLSEMGEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2887..3084
                     /gene="iscX"
                     /locus_tag="ACFMWZ_000440"
     CDS             2887..3084
                     /gene="iscX"
                     /locus_tag="ACFMWZ_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC5934447.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPKSLRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3152..3553)
                     /locus_tag="ACFMWZ_000441"
     CDS             complement(3152..3553)
                     /locus_tag="ACFMWZ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC5934448.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDTKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            3764..4723
                     /locus_tag="ACFMWZ_000442"
     CDS             3764..4723
                     /locus_tag="ACFMWZ_000442"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC5934449.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4791..6101
                     /gene="tilS"
                     /locus_tag="ACFMWZ_000443"
     CDS             4791..6101
                     /gene="tilS"
                     /locus_tag="ACFMWZ_000443"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC5934450.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHCLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            6175..6960
                     /locus_tag="ACFMWZ_000444"
     CDS             6175..6960
                     /locus_tag="ACFMWZ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC5934451.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEGKMPSEEVRRLRAVVPEGKVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGSADVWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLASYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            6970..7167
                     /locus_tag="ACFMWZ_000445"
     CDS             6970..7167
                     /locus_tag="ACFMWZ_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934452.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            7429..7782
                     /locus_tag="ACFMWZ_000446"
     CDS             7429..7782
                     /locus_tag="ACFMWZ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934453.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7794..8099
                     /locus_tag="ACFMWZ_000447"
     CDS             7794..8099
                     /locus_tag="ACFMWZ_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934454.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            8161..9537
                     /gene="mpl"
                     /locus_tag="ACFMWZ_000448"
     CDS             8161..9537
                     /gene="mpl"
                     /locus_tag="ACFMWZ_000448"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC5934455.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIDVYEGFDAAQLDEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HSRWVLAVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGGARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            9744..10796
                     /gene="bioB"
                     /locus_tag="ACFMWZ_000449"
     CDS             9744..10796
                     /gene="bioB"
                     /locus_tag="ACFMWZ_000449"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC5934456.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(10874..11509)
                     /locus_tag="ACFMWZ_000450"
     CDS             complement(10874..11509)
                     /locus_tag="ACFMWZ_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC5934457.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGSHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQIGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            complement(11633..11872)
                     /locus_tag="ACFMWZ_000451"
     CDS             complement(11633..11872)
                     /locus_tag="ACFMWZ_000451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934458.1"
                     /translation="MGVCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            11859..12092
                     /locus_tag="ACFMWZ_000452"
     CDS             11859..12092
                     /locus_tag="ACFMWZ_000452"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC5934459.1"
                     /translation="MQTPKICYAFRTPSAYGQYLAAAGFDYLSFANNHSNDFGAQGIT
                     ATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12155..14014)
                     /gene="ilvD"
                     /locus_tag="ACFMWZ_000453"
     CDS             complement(12155..14014)
                     /gene="ilvD"
                     /locus_tag="ACFMWZ_000453"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC5934460.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTHAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14188..15957)
                     /gene="cysI"
                     /locus_tag="ACFMWZ_000454"
     CDS             complement(14188..15957)
                     /gene="cysI"
                     /locus_tag="ACFMWZ_000454"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC5934461.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILSELDDLIGKWAAGRDTDEGFGDFAIRTGI
                     VKPVLDAPVDFWDASKAVPIARA"
     gene            complement(15984..17798)
                     /locus_tag="ACFMWZ_000455"
     CDS             complement(15984..17798)
                     /locus_tag="ACFMWZ_000455"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC5934462.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSVLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVRRAELKD
                     YKAKNISGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFTASADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARVGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17795..19081)
                     /locus_tag="ACFMWZ_000456"
     CDS             complement(17795..19081)
                     /locus_tag="ACFMWZ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC5934463.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAKVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19120..20043)
                     /gene="cysD"
                     /locus_tag="ACFMWZ_000457"
     CDS             complement(19120..20043)
                     /gene="cysD"
                     /locus_tag="ACFMWZ_000457"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC5934464.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20081..20821)
                     /locus_tag="ACFMWZ_000458"
     CDS             complement(20081..20821)
                     /locus_tag="ACFMWZ_000458"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC5934465.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIAGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(20832..22274)
                     /gene="cysG"
                     /locus_tag="ACFMWZ_000459"
     CDS             complement(20832..22274)
                     /gene="cysG"
                     /locus_tag="ACFMWZ_000459"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC5934466.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQ
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFQLAESCQKPVNV
                     VDDRDYCSFIFPSIINRNPIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENGEGENRVGQAYPALGGLNAGQRAA"
BASE COUNT         4853 a         5843 c         6452 g         5296 t
ORIGIN      
        1 tataaaatcg gcatttcgga tgtaagcaat tttgaaggca gaatgcgcca tttggaaaac
       61 aacggctatg cgaacgttgc cggattggaa cgcatcctcg ccgtcaaaac cgacaattac
      121 aaagaaaaag aaaacctgct ccatgaaatt ttcagcaaaa gcaggatagg cgataccgaa
      181 ttgttcgccg tggacgaaaa ccttgtaaaa cgtttgtttt tatcgcttcg cggcgaaatc
      241 gtgtttccga aaaacgaaac ggcggaatcg gaatttgaaa aaagcgtcca cgaacgcagg
      301 caggaaggga atgccgggtc cggccgcaaa caactgcttg atttggtacg gcgcggacac
      361 cgggaatacc cttacgcgct gccccggctt ttggcgggcg cggcatccta caagccgaaa
      421 aaatcgaaaa tccgcctttt taaagaagca tatttcggca aaagcggcac gaggctgacc
      481 gacgaaattg cagacggcat ccatatttac acctgttttt cgcgggcgga tttggaaaaa
      541 gcctattccg aatatttgga acttttcaaa tccgaatcgg atgccgaagg cagaaagccg
      601 cagtaaggtg caaacagata ccgtacacgt tgaggagcag atatgatggg cgattccgtc
      661 atttattatg tagaacaggc agacaaaccg gtaaaccgtg ccggcgaacg cgcccgcaaa
      721 acattcaaat atttttggcg cgagcttttt tgggaacgcc gccgcatcat tcccgcctta
      781 gattttgcca tggtcaaagc cccttttttc caagacggcg aagacggcga aatttgcgaa
      841 catatgtgga tcgacgatat ttattttgac ggtctttaca tttatggcgt gctgaacaat
      901 gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagcg tttgcgttcc ggttgacgac
      961 atcagcgact ggatgttcgt gtgcaacggc attccctacg gcggctttac catacaggca
     1021 atgcgtggac agatgacgga agaggagcgt accgaacacg atgccgcatg gggaatcgat
     1081 ttcggcgatc ccgggcaggt attgctggtg tatgaagaaa aagaacatcc cgaaaatctg
     1141 gaagagcatc cgatgtgccg gaactgtatt gacgattttc ggcaacagtt gtcccaaaat
     1201 ccggattttc tgcatgagca ggacgaagac ggctatacgc cgcttcatca tgacgccatc
     1261 gcaggaaatg cacttatggt taaagctatg cttgaatacg gcgcaaatcc tgcctcaacg
     1321 acatcggaag gctataccgc cctcgatttt gcccgcctga cgggctggca aaatgttgcc
     1381 gacctgctcg aaccgcgaca ttaggcagac agttttccga aaacgaacac aaacactttt
     1441 tacagaaaga caataaaaat gcccaaaatc accgtacttc cacacacgac attatgcccc
     1501 gagggtgcag tcatcgataa cgcacccgaa ggtaaaaccg tccttgacgt gctgctcgac
     1561 catgatatcg aagtcgatca cgcctgcgaa aaatcctgcg cctgcacaac ctgccacgtg
     1621 attatccgca aaggtttcga cagcctggaa gagccgaccg aattggaaga agacctgctc
     1681 gatcaggctt ggggtttgga agccgattcg cgcctgagtt gtcaggcggt tgtcgccggc
     1741 gaagatttga ttgtggaaat ccccaaatac accatcaacc acgcgcgcga agaacactga
     1801 aaacaggccg tctgaagccg gcacgcttca gacggcattg ttgcgcggat aaggcgcaat
     1861 cgcccgaaaa caggcgttcg tacaggcgga actttcgatt ctatagcgaa ttaacaaaaa
     1921 tcaggacaag gcggcgagcc gcagacagta caaatagtac ggcaaggcga ggcaacgctg
     1981 taccggttta aatttaattc actatatatt gatttttatc ggttttctga cggaacggat
     2041 tggtgcggca tccgaggcgg attactcggg cgcgatgcac gggtatttat cggttttgca
     2101 gccggaaaaa ccgccggcgg gttactttca gggcggcggt cgggcggtat gccggatagc
     2161 gttccacttt cgcttctata ttgattttga ttggttttct gacggaatga cccgatgcgg
     2221 catccgggac ggcttgtgtt ttttcctgcc cgcctgccgg attttcccat ccttgcgtga
     2281 aaccgaaaga gacggcggcg cggcggacaa gctcgagata gcgtccttca agctccgggc
     2341 aggcggcgga cacgctttct gccgtaaccg tgaagccgcc gcccgacccg gcaaggcgtt
     2401 ccgcaatctg cgtgtagatg cgccaacgtt gggcgcgcaa cattgccgca tcgcaaccgg
     2461 cagccgcttt atccaaggca atcaggccgc gcctgctccg gtagtgaagg caaaccgtta
     2521 ctctggagag gatatgctgc ccgtccaata tttgatacag tttgcgtatc agcaaaatca
     2581 ggcggtcaaa ctcccccatc tccgacaggg ggtcgggatg gtcggaagcg gtataaacca
     2641 gtttggacaa ttttgcggca aacatattgt tcatcaatct tccttgtcgg ttgaaacccc
     2701 gctcttcagg gcggtagaat catatttatt tgggagggac aactcctccc agatcaggac
     2761 gacacatagg ctggtgcttg atgtgttgtc cagcgagttg aaacattcag ccatcctcaa
     2821 agggcggcag ttttgccgaa acatattcta cacggcttca atgccggacg ataaaaggaa
     2881 atttatatga aatggaccga tacccggcgc atcgccgaag aactctacga cctgcacggc
     2941 gaaaccatcg atcccaaaag cctgcgcttt acccaattgc gggatttgat tatggcgctt
     3001 cccgaatttg acgacgaccc cgcccgttgc ggcgaacgca tcctcgaagc cgtgcagcag
     3061 gcatggatag acgaggcgga ataagtttcg ggaatgccgt ctgaaatgcg gcggtacgcg
     3121 gttcgtgctt ctgtttgcag cgggaatggt tttaccagtc tccttttttc agcctgtcca
     3181 gttggcggcg gtcgcgcttg gtcggtctgc cgtctggatg ggcggaagtg atgcggctga
     3241 attggtcgag ctgtttgcgc tcttccctca atgctgccgt tttcgtgtct tcttcataca
     3301 gaagccgcgc ctcggatgcc gggcggcgtt ggtggttcaa acctttaacc ttgattttat
     3361 agggaagaga attgagcgtc aggtcgataa tatcgccgat gtctatggtt ttactgtttt
     3421 tgaccttcga gccgtttact tgaaccctac ccagttcgat gtgcttttgc gcaagggaac
     3481 gggtcttgaa aaaacgtgcc gcccaaagcc atttgtccag ccgcatggcg gaagaatcgt
     3541 gcttgtcttt catacgattt tgtttgaaat aattgaattt gtttcaagtt tagcataaga
     3601 tacgccgcct ataactagta tatatgcact aacccactgt tttccatgct gtccgaacac
     3661 aaaaagaggg tgtggaaaag ccgttttgga caataaatta actgcggaat atgcacaaat
     3721 agcgtatgat agcggcagaa tctgttgatg agagcttcat tctatgaaac ctgttttttt
     3781 ggattttgaa caacccatag ccgaactgac caacaaaatc gatgagctgc gtttcgtcca
     3841 agacgagtct gccgtcgata tttcggacga aatacaccgt ttgcagaaaa aaagcaacga
     3901 tttgaccaag tctattttca gtaaacttac acccgcccaa atttcacagg tttcccggca
     3961 tccgcagcgt ccctatactt tggattacat tgaggcactg tttaccgatt ttgaagaact
     4021 gcacggcgac cgccactttg ccgacgatta tgcgattgtc ggcggattgg cgcgtttcaa
     4081 cggacaaagc gtgatggtcg tcgggcatca gaaagggcgc gacaccaaag aaaaaatccg
     4141 ccgcaacttc ggtatgcccc gtcctgaagg ctaccgcaaa gccctgcgcc tgatgaagac
     4201 ggcagaaaaa ttcggcttgc ccgtcatgac ctttatcgat acgccgggcg cgtatcccgg
     4261 catcggcgcg gaagaacgcg ggcagtcgga agccatcggc aaaaacctgt acgaactgac
     4321 gcgcctgcac gtccccgttt tgtgtaccgt catcggcgaa gggggctcgg gcggcgcgtt
     4381 ggcggtcgcc gtaggcgatt acgtcaatat gctgcaatat tcgacctatt ccgttatctc
     4441 ccccgaaggc tgcgcttcga ttttgtggaa aaccgccgaa aaggcggcgg atgcggctca
     4501 ggctttgggc attactgctg accgcctgca aaagctggac ttggtcgata ccgtcatcaa
     4561 agaaccattg ggcggcgcgc atcgggattt cgggcaaacc atgaaaaacg taaaagccgt
     4621 tttggaaaaa caactgcacg aagcgcaaag catcccgctt gccgatttgc tttcgcgccg
     4681 tttcgaccgc attatggctt acggcaaatt ttcggaacaa taattcaggt agaacaagca
     4741 gcaagcagtt tgtctgaaac tgcttgcttt ttctttatcg ggacggaacc gtgctgactt
     4801 tagatgcgtt tgagcaatgc ttgaaggatt gttttcctca aggtctgaat ggaaaaaaaa
     4861 cagcggtggc attaagcggc ggcttggatt ccgtcgtttt gctgcatctg cttgtccgcg
     4921 ccggaaaaaa gggcggtttt attccggatg cattgcatat ccatcactgc ttgagtcccc
     4981 gtgccgacga ttgggcagat ttctgccaaa actattgcga tatgctcggg gtggggctgg
     5041 aaacggttaa ggtctgcgtg gaaaaaaacg gtttgggcat cgaggcggcg gcgcggcaaa
     5101 agcgttatgc cgcgtttgcc gaaaaaggct ttgacgtttt ggcgttggcg caccacaggg
     5161 acgatcaaat cgaaaccttt atgctggcag tcgctcgcgg cggcggtttg cgcgctttgg
     5221 cggctatgcc caccgtccgc ccttttgggg aaaaaggcat catctggcgg cctttgctgc
     5281 ctttttcgcg ccaagacata tgggattatg cccaaaaaca cggtttgccg aatatcgagg
     5341 acgaaagcaa tagcgatacg gcttatttgc gaaaccgctt ccggcaccgt attttgcccg
     5401 aactttcggc gcagattccc catttcgggc ggcatgtact gaacaatgtc cgcgctttgc
     5461 aggaagattt ggctttgttg gacgaaatca tcggtcagga ctgccgttgg gtctgtgagg
     5521 aaggcagttt cgatacggtg cgttggcagg cgttttcccc acgccggaaa acccatattt
     5581 tgcggtattt tttgaaggaa aacggcatcc ccatgccgaa tcagaatgcc cttgccgaca
     5641 ttgcccgggt tttgacggag gcaaaaaccg gacgttggaa cttgcaaggc tttgaattgc
     5701 atcattatgc aggcaggctg tttgtgttcc gactggaaaa aacggataaa ctgcggtttt
     5761 tgaaagacag gcagataagc ggaaatttaa gggaaatatt gacggggcag ggatttgttt
     5821 taaaacgtca tccgtttggg cttcctgagc atcttttgga gcaggacgga attttgagga
     5881 cggtagcggt atcggatacg ttggctgtcg gcggcatcca taaggatgtg aaaaaaatcc
     5941 ttcaggggaa acgggttttg cctgtcctgc gcccaatttg gccgcttgtt gccgacagcg
     6001 gaaaccgtcc attggcgttg gcaaactgtt gtgcggattt ccaatactcg gtttcagacg
     6061 gcattttgcc cgtccatcct gactttccca ttttattttg ataatatcgc aaacagattt
     6121 cggcggcgtt cagacgggta ttgtccggtt gcatatttct aaaaggcttg tgaagtgaaa
     6181 cacatcagtt cgaccaataa tgaacacatc cggcacctgc accgcctgtt gtcgcaagga
     6241 aagttcagac ggcaatacgc ccaaaccgtt ttggagggcg tgcacctgct tcaggttttc
     6301 ctgcaatccg gcagaaaacc ggtcggggta tatattcccg agggcaaaat gccgtctgaa
     6361 gaagtccgca ggctgagggc ggttgtgccg gaaggcaagg ttttttccgt ttcagacggc
     6421 atattgaaaa aaatcagcag cttgacttgt gcggatgatg tgcttgcgct gattgatatt
     6481 ccggatgcgg gtgctttgcc tgccggcggc gactgcgtgg ttttggacgg cgtgcaagac
     6541 ccgggcaatg tcggcacggt gttgcggagc gcggcggcgg caggggtagg tacggtcgtt
     6601 ttgggcaggg gttcagcgga tgtttggtcg cccaaggtat tgcgcgcggg gatgggcgcg
     6661 catttcttgt tggacattta ttcgcaggcg gatttggaaa tatggttggc gagctataaa
     6721 ggccgtgtgt ttgccaccgc cttgcgagag gaaaagcagg cggttttgta cggcgaagat
     6781 ttgtgcgaac cgacagcctg ggtgtttggc aacgaaggcg cgggggtcgg taaagcggtt
     6841 ttggacaggg cggacaagtg tgtcaggatt ccgatgcacg atgcaaccga gtctttaaat
     6901 gtcgcgatgg cggcgacaat ctgcctgttt gaacagatgc ggcagcggtc ggcgtattga
     6961 ggaaaagaaa tgccgtctga aaaaatctat tacggcgtat tgattttctt atgtatcgct
     7021 tctatgctgc tgtcgccgtt tttttatgcg ggtgctttga agcccaagaa ggcggcattg
     7081 cggaaggacg ggcagtggaa actcatctta ttgtccaata ccgtggcggc ggcggttttg
     7141 ttttggatgt ggtggaaatg gttttgacag atattgctca aaattgggtt aatggaatcc
     7201 gaacaaataa ataatttggt aaaaaatttg ttaaatcaac ggattaaagt tttgtggaaa
     7261 acaaaacagc tctaagcaaa tagggcgttt gtcggtaaat acggaagagt tgcggcatta
     7321 tcgggcatct ttaacaagta gtgccgtctt ggcaggcaat cggtttttat gggcagcttg
     7381 caaaatcgcg gatataaaat tgcgaatcgg ttaaagtgtg gggacgctat gaaaaattgc
     7441 gaattttttt atgacccgac aagggcaatc tatgatagcg gtgcagatta cttaactagg
     7501 gaaaaacata gattagtcgt aattgcaaat agtgcttggg ggctattgct taatttatct
     7561 tgttattatg acgaggtttt ggaaaagcgg aaaataccgt tcggcaaaca ggaaattgat
     7621 gacgatatgg acaaagtgtc cgcccttaag cggaagttta aagatatttc tgaaatcaaa
     7681 gtaggggatg gttgggaata cccgttcaat tatgagcagg gaatgaaaga attagatgaa
     7741 gtgctattga aatacattcc cttttttgaa gaagaacgat aaaggaggtt gatatgcgcg
     7801 tatctaaaat aattggaagt atgttgcttg ttacagcggt tcagaccgta ttttcggcaa
     7861 atgtttacgc gtgccgccat aatggtaaaa ccagttacag ccaaactccg ggaaaacatt
     7921 gtaccaacgc gggtttgggg cgggatcggg tgtacagttc ggttagaccg gcagtaaaag
     7981 acagggcgga agacgcaggg gtcggcgatt attcggacac ggtgagggac gaacacgtcc
     8041 aaaatccgag agagaatacg cataaggatg cttcgaatac cggtgcaaag acgcattgat
     8101 tgaaaccgaa tcagccatga cggcgttgaa taatagaatt tgaacgatta gggaactctg
     8161 atgaaacaca tccacattat cggtatcggc ggcacgttta tgggtgggat tgccgccatt
     8221 gccaaagaag cagggtttga agtcagcggt tgcgacgcga agatgtatcc gccgatgagc
     8281 acccagctcg aagccttggg catagacgtg tatgaaggct tcgatgccgc tcagttggac
     8341 gaatttaaag ccgacgttta cgttatcggc aatgtcgcca agcgcgggat ggatgtggtt
     8401 gaggcgattt tgaaccgtgg gctgccttat atttccggcc cgcaatggct ggctgaaaac
     8461 gtgctgcaca gccgctgggt gcttgccgtt gccgggacgc acggcaaaac gaccaccgcg
     8521 tctatgctcg cgtgggtctt ggaatatgcc ggactcgcac cgggcttcct tatcggcggc
     8581 gtaccggaaa acttcagcgt ttccgcccgc ctgccgcaaa cgccgcgcca agacccgaac
     8641 agccaatcgc cgtttttcgt cattgaagcc gacgaatacg acaccgcgtt tttcgacaaa
     8701 cgctccaaat tcgtgcatta ccgtccgcgt accgccgtgt tgaacaatct ggaattcgac
     8761 cacgccgaca tcttcgccga tttgggcgcg atacagaccc agttccacca cctcgtgcgt
     8821 accgtgccgt ctgaaggcct catcgtctgc aacggacggc agcaaagcct gcaagacact
     8881 ttggacaaag gctgctggac gccggtggaa aaattcggca cggaacacgg ctggcaggcc
     8941 ggcgaagcca atgccgatgg ctcgttcgac gtgttgcttg acggcaaaaa agccggacac
     9001 gtcgcttgga gtttgatggg cggacacaac cgcatgaacg cgctcgccgt catcgccgcc
     9061 gcgcgtcatg ccggagtcga cattcagacg gcctgcgaag ccttgagcac gtttaaaaac
     9121 gtcaaacgcc gcatggaaat caaaggcacg gtaaacggca tcactgttta cgacgacttc
     9181 gcccaccatc cgaccgccat cgaaaccacc atagaaggtc tgcgtcagcg cgtcggcggc
     9241 gcgcgcatcc tcgccgtcct cgagccgcgt tccaacacga tgaagctggg cacgatgaag
     9301 tccgccctgc ccgaaagcct caaagaagcc gaccaagtgt tctgctacgc cggcggtgtg
     9361 gactggaacg tcgccgaagc cctcgcacct ttgggcggca ggctgcacgt cggcaaggat
     9421 ttcgatgcct tcgttgccga aattgtgaaa aacgcccgaa ccggcgacca tattttggtg
     9481 atgagcaacg gcggtttcgg cggaatacac ggaaagctgc tggaagcttt gagatagccc
     9541 gggcgatgcc gtctgaaagc ccttcagacg gcatcgcccg gctgcgcggc acaaaggcgg
     9601 aaaaaccgtt tgccccgtat tttcaaacgc gttacacttg ccgccgctgt tttcagccat
     9661 ttgattaccc gcaaccgccg tcattgcgcc ggcggtttgc ctgtcagcgt cattgcgccg
     9721 ctgtaaatac gaaagaacac attatgaccg tatcccccgt cgccttgcgc cgtaagaccg
     9781 agtgcaagcc tcatcccacc gcgcgctatt ggaaaaaatg tgatgtcgaa gccctgttcg
     9841 gacttccctt cctcgacctc atttaccaag ccgccgaaat ccaccgccaa aatttcaacc
     9901 cgcgcgaaat ccagctttcc acgctgttgt ccatcaaaac cggcggttgt cccgaagact
     9961 gcgcctattg tccgcaatcg gcgcaccaca ataccaatct gggcaaagag cagatgatgg
    10021 atgtggatga aatcgtcgaa aaagccaaaa tcgccaaatc gcgcggcgca agccggtttt
    10081 gtatgggcgc ggcgtggcgc ggccctaaac ccaaagacgt ggagacggtt tccgcaatca
    10141 tcaaagccgt caagggcttg ggtatggaaa cctgcggcac gttcggtatg ctcgaagaag
    10201 gtatggcgga agacttgaaa gaggcgggct tggattatta caaccacaac ctcgacaccg
    10261 accccgaccg ctacaacgac atcatccaca cccgccaaca cgaagaccga atggacacct
    10321 tgggcaaagt ccgcaacgcc ggtttgaaag tctgctgcgg cggcatcgtc gggatgaacg
    10381 aaacccgcgc cgaacgtgcc gggctgattg ccagtctcgc caatctcgac ccgcagcccg
    10441 aaagcgtgcc gattaaccgg ttggtcaaag tggaaggcac gccgcttgcc gatgccgaag
    10501 atttggactg gacggaattt gtccgcacca tcgccgtggc gcggattacg atgccgcaaa
    10561 gttatgtccg gctgtcggca gggcgcagca atatgccgga agcaatgcag gcgatgtgct
    10621 ttatggcggg cgcgaactcg attttttacg gcgacaagct gctgaccacg ggcaatcctg
    10681 atgaggacgg cgacagaatc ctgatggaaa agctcaacct gtatcccttg cagtttgaac
    10741 cggaaggcga ggtcgccgaa gtggaaaaag cctctgggat taaagtggat tattgacgat
    10801 tgaaaaatgc cgtctgaaac ccggaaaaag gctttcagac ggcatttgtc cggacggcat
    10861 ttccaatctc tttttaccgg cgcgtgatgc tgccgtcggg cgagacatcc agcccgttcc
    10921 ccttggggaa atcgctgcgg ttcagataaa taatccgccc gataacggtt ttctcggggt
    10981 cgattttggg gattttatcg ccgatttgag tgatggggat aatgttgccg gaaacgaacc
    11041 gcccctgttt gtcggtgata attttgaaaa tcggcgcaat gccgctgatg ccggaggtgt
    11101 tgattgcgcc gtaggtggca aagttgccgc cgctgtagga gatgaagcgg tcgcggtaaa
    11161 gttcgacggc gcgagtaacg tgcgacccct gcccgaatac gacatccgcg ccggaatcga
    11221 cggcaagccg tgcaaactcg acgacgttgc ccctgttttc cccatagaag atttcggtat
    11281 cgaacggcag gtgttccgcc tgtttccctt ccgcgccgcc gtggaacatc acaatgacga
    11341 tgtcggcttt ctgtttggtt ttggtaatcc gttttctaac tttggcataa tcgttcagtt
    11401 tgacggcggc aaggttgggg gcgaaggaga cgaagccgga tcttacgccg tttttcttca
    11461 ggatggcggt ttcaaacctg ttttcgatgc ccgaatattt gatgttcaat tcgtcaaggt
    11521 tcgcccttta tgccgtttcc gcaccgcaaa ccgccggggt caagccctcg gcgcatcctg
    11581 ccggaacgga atccccgtgc tgccgattga cggttcaaac cccgcgcccg tttcaaatac
    11641 cggcgatgtg gacggacagg atgcgcctga cgaaaagaca gccgataccg tttccattat
    11701 cggcgtgggc gacattatgc tcggcagcaa ttatccggtc gattacctgc ccgataccaa
    11761 tattctgaaa aacgtcgaat ctgccttgca agacgcggac attaccgtcg gcaacctcga
    11821 aggcacgctg tttgacgaag gcggtacgcc gaaaaaatgt gcaaaccccc aaaatatgct
    11881 atgcattccg aacgccctcc gcatacgggc aataccttgc cgccgcggga ttcgactacc
    11941 tcagcttcgc caacaaccac agcaacgact tcggcgcgca aggcatcacg gcaacggcgg
    12001 cgagcggcag ctcttttaca tactcgatcg cgctaaagcc gctgccgata acgaggccaa
    12061 aattgcggaa aataccgcca tcgcccagat aaatttgtcc atcatcagac ctttactgtt
    12121 cagacgagac agcatttgcc gcacgttttg gggcttatct ttcgatttgc gctacgtcgc
    12181 gcaccgcgcc tttgtcggcg gaagtcgcca tcgcgccgta agctcttaat gcggcggaga
    12241 cgtagcggtc gcggttttta ggcttccatg ctttgctgcc gcgcgcttcc atttcggcac
    12301 ggcgtgcagc aagctcttca tcggaaatgg caaggtggat gctgcggttg gggatgtcga
    12361 tttcgacggt atcgccttcg tgtaccaaac cgatcgcgcc accttccgcc gcttcgggcg
    12421 aggcgtgtcc gatggacaaa cctgatgtgc cgccggagaa gcgtccgtcg gttaagagag
    12481 cgcaggcttt gccgaggcct ttagatttca ggtaggaagt cggatacagc atttcctgca
    12541 tgcccgggcc gcctttcggg ccttcgtagc ggatgatgac gatgtcgccg gcgacgattt
    12601 ggttgcccaa aatgccttct actgcgtctt cttggctttc aaacacgcgg gcgcggccgg
    12661 tgaatttgag gatgctctcg tccacgcctg cggtttttac cacgcagccg cgctcggcga
    12721 tgttgccgaa caagaccgcc aaaccgccgt cttgcgagta ggcgtgtgcc acgtcgcgga
    12781 tacagccttt ttcgcggtcg aggtcgaggg ttttccacat acggttttgc gagaacgctt
    12841 gggtggtgcg tacgccgccg ggcgcggctt tgaagcgttc gatggcgtgg gtgttttcag
    12901 ggttggttac gtcccatttt tcaatcgcgt ctttcagcgt cggcgcgtgg atagtgtgca
    12961 cgtcggtgtg cagcttgccc gctttgtcca attctttcag gatggcgaaa atgccgccgg
    13021 cgcggtgcac gtcttccata tagtagtcgt ggttgttggg tgcggttttg cagatgcagg
    13081 gcacaacgcg gcttaagcgg tcgatgtctg ccattttgaa atccacgccc gcttcgttgg
    13141 caacggcgag taaatgcaaa atagtgttgg tgctgccgcc catcgcaata tccatcgtca
    13201 tagcgttttc aaacgctttt ttggtggcaa tgctgcgcgg taacacggtt tcatcgtttt
    13261 gctcgtaata gcgtttggtg atttcgacaa tcatacggcc ggcttcgagg aacagttctt
    13321 tgcggccgac gtgggtggcg aggtaagaac cgttgcccgg cagggacagc ccgagtgctt
    13381 cggtcaggca gttcatcgag tttgccgtaa acatacccga acacgagccg caggtcgggc
    13441 aggcgttttg ttcgacttcc tcgacttgcc ggttgctgac attgtcgtcc gccgattcaa
    13501 tcatcgcgtc aatcaagtcc aaacggcgtt cgggctggat gtttgccacg ccgataactt
    13561 tacctgcttc catcggaccg ccggaaacga agatggtcgg gatgttcagg cgcatcgcgg
    13621 caatcagcat gcccggggtg attttgtcgc agttggaaat gcacaccagc gcgtcggcgc
    13681 agtgggcatt gaccatatat tcgatggagt cggcaatcaa atcgcggctg ggcagggaat
    13741 acagcatacc gctgtgtccc atagcgatgc cgtcgtcgat ggcgatggtg ttgaattctt
    13801 tggcgatggc gcccgccttt tcgatctcgc gggcaaccag ctggcccata ttgtgcaggt
    13861 ggacatgacc tggcacgaat tgggtgaacg agttggcaac ggcgataatc ggtttgccga
    13921 agtcggtttc catcacgccg gtggcgcgcc acaatgcgcg tgcgcccgcc atattgcggc
    13981 cgtgggtgga ggttttggag cggtattctg gcatagtgtg tttccttgtg cctataccgt
    14041 ctgaaagaca gggctgtttc agacggtatc gggtacggtt ttttagagtg ggaaaagagg
    14101 gtattttata ccaagtatcg gaattttgcg gaatggaaac gcgtgcggca aaaaaggaca
    14161 tcccccgcag gaatgcgggg gacggtttca ggcgcgcgca atcgggacgg ctttggatgc
    14221 gtcccaaaaa tcgacgggcg catccaacac gggtttgacg atgcccgtcc gtatggcgaa
    14281 atcgccgaaa ccttcgtcgg tatcgcgtcc tgccgcccat ttgccgatca ggtcgtctaa
    14341 ttcggaaagg atttccggca gggtaatgtt ttctttgtag aggcggggga tgcgtacgcc
    14401 ttcgcggtcg ccgccgatgt ggaggttgta gcgtccgacg gctttgccga ccagtccgat
    14461 ttccgccaac atcgcccgtc cgcagccgtt cgggcagccg gtaatgcggg taacgatgta
    14521 gtcgtccgac gtgccgtgtt tcgccataat cttatccagc tcgccgatga agtccggcag
    14581 cacgcgttcg gcttccgcca ttgccagcgg gcaggtcggg aaggaaacgc aggacatcgc
    14641 attttcgcgc agtttgctga caccgtcgtg tatcagcccg tatgagcggg cgagctgttc
    14701 gatccgggct ttgcctcctt cgggtacatt tgccacgatg aggttttggt tggcggtgat
    14761 gcggaagtcg cctttgtgga ttttggcgat ttccaacacg ccggtcagaa gctgtttccc
    14821 gccttcgtca accaaacgcc cgctttcgat gaaaagggtc aaatgccagt tgccgtctat
    14881 gcctttcacc cagccgatac ggtcgccgcg cccggtaaat ttgaacgggc gtacgggttc
    14941 gaacggcata cccatacggc gttcgacttc cgcgcggaag ttgtccaagc ccatattttg
    15001 aatggtgtag cgggtgcggg cgtttttgcg gtcgctgcgg ttgccgaagt cgcgctgtgt
    15061 ggttaccacc gcttcggcgg ccttcagcgc gtgttccgga ggcacgaaac ccagttccag
    15121 tgaaatgttc ggataggttt tggtgttgcc gtgttccatc gaaagcccgc cgcctgccaa
    15181 aacattgaag ccggcaagct gtccgttacc gtctgaaacg gcgacgaaat ccaaatcgtt
    15241 gccgtagcag tccacatcgt tcaagggcgg gatgacgact gcggttttga atttgcgcgg
    15301 cagataggtt ttgcccaaaa tcggctcgtc ttcttgaagg aagtcgtcgg aactttgaac
    15361 ttttttgccg tccacccaca catccagata accgcgcgtg cgcggcagca agtgttcgga
    15421 aatctttttc gcgtattcgt aagcctgccg gtgcagttcg gactcgatcg ggttggacgt
    15481 gcaaagcacg ttgcggttca tatccgccgc cgtggcgatg gaatccaaac ccagtttgtg
    15541 caagaggcgg tgcatcgtct gcaacttggc tttcggcacg ccgtgaaatt ggaaggtttg
    15601 ccggttggtc agccggatgg agcggtaatg actgttttcc cgggcaaatt tgtccagttc
    15661 tatccattgg gacggtttaa tgatcccgcc cggcagccgg cagcgcaaaa gcataaattt
    15721 caagggctcg agttttgcct cggcgcgttc ggcgcggatg tcgcggtcgt cctgctcata
    15781 cataccgtgg aagcggatga gttggaagtt gtcgcctttg aagccgcccg tgagcgggtc
    15841 tttcaaatcg tccaaaatcg tgccgcgtaa aaaattgctt tcggttttca gacgttcgtt
    15901 gtcggatagc ggtttttctt gccacgccaa accttttgtc ttggtctgta cggtcatttt
    15961 gtgttcctcc cgattatatt taatcaataa acatcacgct gatagcgttt ttcttcgcgc
    16021 agcatatcca aatattcttc tgcgccctct tcgtccaaat gtcctgcccc gataatcaca
    16081 tccagcaagg cggcttccac gtcttttgcc atttttgccg catcgccgca cacatagata
    16141 tgcgcgcctt cctgcagcca ttgccaaagt ccttccgcct gttcgcggat tttgtcctgc
    16201 acatagattt tttcttcctg atcgcgggac caggcgaaat cgtacctgtg caggaagccg
    16261 tctttggcaa actgctgcca ttcggtctga tagaggaagt cggcggcaaa atgcggattg
    16321 ccgaaaatca gccagttttt gccttccgca ttttctgcgg cacgttgttg gacgaaagcg
    16381 cggaacggtg cgacgccggt gcccgagccg atcatcacaa tcggcttgcg gctgtcttcg
    16441 ggcagcctga agccgtcgtt gcgttccaca aacacgcgca ccgtgccgtc ctcttccagc
    16501 cggtcggcaa ggaaacccga tgcgccgccc accctggcgc gcccttcgtg ttcaaaacgc
    16561 accgcgccga cggtcaggtg cacttcgtcc cccacttccg cctgcgacga ggaaatcgaa
    16621 tacaggcgcg gcgcaagcgg gcgaagcagg ccggcgaatt gttccgccgt cagttttgcc
    16681 gggaagcggt gcagcacatc ggcaatcggc gtgctttgca caaagccttg caaaacggcg
    16741 ttgtcggcag caatacggtc gagttcgtca tcatcggcga acggggcata gcctttgaca
    16801 aaggcggggg tgttttgcgt gagttcaaaa tgggataaca gtgcggaggc aaccggcagg
    16861 gtttttccgc ccgcctgtat ttccgttgcc ggatcgatgc cgagcaggtc taggatttcc
    16921 ctgaccagtg ccggatcgtt gtcaaaccaa acgccgagcg cgtcgcccgg gaggtagtgc
    16981 aaatccgaac cgctcaaatc gatttcgatg tggcgcacgt ctttatcgga ttggcgggcg
    17041 gtgattttct gattggccag cagggcggcg ggaaaggggt ctgccttgca gtacctgcca
    17101 tccggtgccg tctgaaggcc ggcggggggc gttgtctgcg gcgcgggcgt tgcccggttt
    17161 tttgcggctt cttcttttaa gcgcgcgaca atgcggcctg tccatccgtc tgcggaggcg
    17221 gtaaagtcca aatccgcatc aacgcgttcg agcaggcgtt tcgcccccaa ttcttcaaaa
    17281 cgtttgtcga aatctttgcc cgcccggcag aaattcggat aggagctgtc gcccaaaccc
    17341 agtacggcaa attggagttt gtccaatttc ggggcttttt tgccgttcag cagtttgtgc
    17401 agcacgacgg cttcttccgg cggttcgcct tcgccttggg tggaggtaac cagcagcagg
    17461 cggcgttcgc cggagatgtt tttcgcctta tagtctttca gttcggcgcg cctgacttgg
    17521 atgccggcgg cttccaggct gtccgccgct ttgtcggcaa cggatttcgc attgccggtt
    17581 tgcgaggcgg aaaggacggt tacggaaaaa ggttctgccg ccggcaatgc cgtctgaagc
    17641 gcgggcagtc ctgcagatgc cccgtttcct gcttttgccc aagcgtagcc ggacagccac
    17701 gcccattgtg ccgcgtccag caccgacagg agctgcgtga tttcgggcgg cagaggcggt
    17761 aatggcggat ttgtgttctg catatcgtgt tcactcataa aatcatacct gccgcaacag
    17821 tgccgtatgt cgcttcgtct atcaggataa acgaaccggc ggcggtgttt tccgcataag
    17881 gcgttgccgt aacgggtttt tgaaggttga tgcggacttt ggcaatgtcg ttcatcttca
    17941 aggattccgc gccggcctct tgttccagcg tgcggacatc taaaacgctt tcaatttccc
    18001 cgacttttgc cggcacggtt tgcgtgccgt gcttgagcag gtatttgcgc gcggtgttga
    18061 gcggacgttc gtcaaaccag caaagcgtgg cttccagatg tttttgcggg gcgagcgggg
    18121 aatttttatc gacaaaaagg tcgccgcgcg aaacatcgat gtcgcggtcc agccggaggg
    18181 ttgccgcctc gccggcaaaa gcctgcgcca cttcgccttt gggtgtgatg atttcagaca
    18241 ctttggcggt cagcccgttc ggttcgatgc ggacggtttg cccgacggcg accgaaccgc
    18301 gttcgatgcg cccctgatag cctcggaaat catcggcctt gtcggcatct tggcggacga
    18361 ccagttgcac ggggaaataa aaatcgtcgg cggtgcggct gacttcgtcc gcccccggca
    18421 gggtttccaa aatggacaat aagggttcgc ctttatacca aggcatattg ccgccggggt
    18481 aaacaatgtt gtcgcccaag agtgcggaca ccggtacgaa atgcacgtct ttcaaaccga
    18541 gccgtccggc gagtcggcgg tatgcctcca caatggcgtt gaatttgtct tcgctgtaat
    18601 ccagcaggtc cattttgttg accgccacca caatatgcgg gcagtcgagt tggcggagga
    18661 tggcggaatg gcgtttggtc tgcggcagaa gctgcaaggg ctgtgcgccg aaatccagtt
    18721 gggatgcgtc aaccagcacg actgccgccg aagcggtgct tgcgcccgta accatattgc
    18781 gcgtgtattg ttcgtgcccc ggcgtgtcgg cgatgatgaa tttccgtttc gccgtggaaa
    18841 aatagcggta tgccacatcg atcgtaatgc cctgttcgcg ttcggcttcc agtccgtcgg
    18901 tcaggatgga gaagtctatg gtttctttca aacctttgct tttgccggat tccaaggttt
    18961 tgatttggtc ggacagcagg gctttgctgt cgtagagcag ccgtccgatc agggtgcttt
    19021 tgccgtcatc gacgctgccg gcggtaatga agcggagcgg ggcctggtgt tgtgccgtca
    19081 tattttcttc ctcatatctg cttaaagggt ttttgaaatt tagaaatagc cttctttttt
    19141 gcgtttttcc attgccgcct cgcttgcctg atcgtccagc cgggtcgcgc tgcgttcgga
    19201 aatgtcggca accgctgttt ctctgataat ctccgtcggc gtggacgcgg tgctttctac
    19261 cgggcaggtg cagctgatgt cgccgacggt gcggaagcgg acatcaagga tttcggaggt
    19321 ttcagacggc attttcgggg tgagcggcgt tacggggacc agcagccccc tgcgtctgac
    19381 cacttcgcgc ctgtggctgt aataaatcgg cggcagctcg aggttttcgc gggcgatgta
    19441 ttgccagatg tcgagttccg tccagttgga aatcgggaag acgcgcatat tttcgccttt
    19501 gtgcagcctg gtgttgtaca gcgaccacag ctcggggcgt tgcgccttcg gatcccattg
    19561 tccgaactcg tcgcggaacg agaaaatccg ttctttggcg cgggcttttt cttcgtcgcg
    19621 ccgcgcgccg cccataagcg cgtcgaagcc gtttgcctcg atggtttcca acaaggtaac
    19681 cgcctgtgcc gcattgcgcg aatcggtttc tttgcgtaag accaccgtgc ctttggcaat
    19741 ggagtcttcc acgcgcccca ctatcaggcg ggcattgagt tttgccgcct gcgcgtcgcg
    19801 gaaagcaatc acttcggggt agttgtgccc cgtgtcgata tgcaccagcg ggaagggcag
    19861 ttttaccggc ctgctgccca gccggaaggc tttgcaggcg agggcgagca ggaccacgga
    19921 atctttgccg ccggaaaaga gcagggcggg gttttcgcat tccgccgcca cttcgcggat
    19981 aatgtggatg gattcggatt ccaaccagtc gagttgggcg ttgttcggtt cggttttcgt
    20041 cataccatat tccttatttc ttctgtctga tatttatgaa ttatttgtgc agcccgcatt
    20101 ctttgctgtt tctgccttcc caccaccacc gcccggcgcg gatgtcttcg cccgccttga
    20161 cggggcgggt gcaggggtcg cagcctatgc tgggaaatcc ttgccggtac aaatcgttgt
    20221 aaggcacatt gttggcgagg atgtatgccc acacgtcgtg ttccgaccag tcgaaaatcg
    20281 ggttgtattt gccgatgccc cgtccggcat cgtattcggc aaacggcagt tccgtgcgtg
    20341 tggcggattg ttcgcggcgt tgcccggtaa gccaggcgtc cgcgcctgca atggcgcggt
    20401 tgagcggttc gatttttcgg atgcggcagc attcgcggcg cgcttcaacg ctgtcgtaaa
    20461 aggcaaacct gcctttgctt tccacataac ggtcggcatc ttctcgaacc ggccggaaac
    20521 gctttatccg caaatgggga tatgcgcgtc cgagcctgtc cagcaggttt agggtttccg
    20581 tgtggagcag ccccgtatcc aaggtaaaaa tgccgatatt gaggttttcg ccggcgataa
    20641 ggtcggtaat caccatatct tctgccgcaa ggctgctggc aaaccgtgca tcccggtggc
    20701 tgccggcaat ccggtgcagg cgttgtttga gggtttccgt tttttccgca agggcggttt
    20761 cgccgccgga tccgatatgc ggtatctgcc acagggcggg tttgaacagt gtcgtttcca
    20821 tttttcccgc cttatgccgc ccgttgtccg gcattcagtc cgcccaatgc gggatacgcc
    20881 tgcccgaccc ggttttctcc ttcgccgttt tcaccgaacc aggcgagttt ttcgtgcagc
    20941 cccaccactt cgcctatgac aatcaatgcc ggattcggcg cggtttcggc gagttcggca
    21001 aggttggcga gcgtgccggt tgcggttttt tgagccggca gcgtgccttg gctgatgacg
    21061 gctgccggcg tgtcgggcga gcgtccgtgc tgttgcagcc gttcggcaat cagggcggct
    21121 ttgagcgcac ccatataaat caccaaggtc tggcggctgc gggcgagggt ctgccattcg
    21181 atgtcgggcg catccgcctt gcggtggccg gttacgaaaa ccgcactttg ggcataatcg
    21241 cggtgcgtga gcgggatgcc ggcataggcg gtcgcgccga cggcggcagt aatgccgggg
    21301 acgaccgaaa acggaatctg atggcgtgcc aaggtttcca attcttcgcc gccgcgtccg
    21361 aacacgaaag ggtctccgcc tttcaaacgc accacgcgcc tgccttcgcg ggccagcctg
    21421 accataagcg cattggtgtc ctcttgcggg gtgcggtcgc cccgggcgcg cttgccgaca
    21481 aaaatccgtt ccgcatcgcg gcggacgagg gacagtatgc cgtctgaaac cagcgcgtcg
    21541 taaagcacca cgtctgcctg ctggatttcc tgtagccctt tgagcgtcag cagccccgca
    21601 tcgccgggac ccgcaccgac caaagacaca aaaccgcctt gataattttg ttccaactgc
    21661 tttgccaact cctgttcggc ttgggcggtt tgttgatttt taacgagggc ggcaaaacgg
    21721 ccgttgaact gcttttccca aaaacggcgg cgttcggtaa cggatttcag tttggctttg
    21781 accgcatcgc gccatcttcc ggagatttcc gccatatcgc ccaaagaatg aggcagcagg
    21841 gcttccagtt tttcacgcag caaacgggcg aggacgggcg cgctgcccga actggaaacg
    21901 gcaatttgaa tcggattgcg attgataatc gaaggaaaaa taaaactgca ataatcgcgg
    21961 tcgtccacca cgttgaccgg tttttggcag ctttcggcaa gctggaaaac gcgccggttg
    22021 agggcttggt cgctgcttgc cgcaatgatg aggaaaactg tgcggatgtg ttcggcacga
    22081 aattcttcgg caagccacag gattttgttt tctgccgcca gcgcacttaa ttcagcattc
    22141 aactgatttg caacaaccct gacttgcgcg ccggcatcca gcaacaggct gattttgcgc
    22201 gcggcgaccg aaccgccgcc tacgaccaat acggggcggc cggcgaggtt ggcgaaaatg
    22261 gggaaataat tcactggcga actcctttgc tgtttgcccg caccttgttt ccgatatggt
    22321 gcgtcgcggt atttttgtcg gaacgcgagt cattttagac aaaaggattt ccccggttaa
    22381 ataataaaaa ggtatttgtt agaagctgaa agctatatgg gggcggatgc ggatgcggcg
    22441 gttt
//
LOCUS       DAXHKX010000013        22326 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHKX010000013 DAXHKX010000000
VERSION     DAXHKX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.013"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            1..97
                     /locus_tag="ACFMWZ_000460"
                     /pseudo
     CDS             1..97
                     /locus_tag="ACFMWZ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(118..1032)
                     /locus_tag="ACFMWZ_000461"
     CDS             complement(118..1032)
                     /locus_tag="ACFMWZ_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC5934467.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1288..2757
                     /locus_tag="ACFMWZ_000462"
     CDS             1288..2757
                     /locus_tag="ACFMWZ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC5934468.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3009..3377
                     /locus_tag="ACFMWZ_000463"
     CDS             3009..3377
                     /locus_tag="ACFMWZ_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934469.1"
                     /translation="MNKTLSILPVAILLGGCAAGGNTFGSLDGGTGMGGSIVKMAVGS
                     QCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTRN
                     QALAALTAKTVSACFKHLYR"
     gene            3450..4859
                     /gene="thrC"
                     /locus_tag="ACFMWZ_000464"
     CDS             3450..4859
                     /gene="thrC"
                     /locus_tag="ACFMWZ_000464"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC5934470.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWTEVAAGKGFDLQFALDKVGGKYGFTSSKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4905..5699
                     /locus_tag="ACFMWZ_000465"
     CDS             4905..5699
                     /locus_tag="ACFMWZ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934471.1"
                     /translation="MTLPMQETRFSILLDELAAKQKAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAARIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5887..6663
                     /locus_tag="ACFMWZ_000466"
     CDS             5887..6663
                     /locus_tag="ACFMWZ_000466"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC5934472.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7422..9893)
                     /locus_tag="ACFMWZ_000467"
     CDS             complement(7422..9893)
                     /locus_tag="ACFMWZ_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC5934473.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVEGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGITHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRQIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKMPSEQ"
     gene            complement(9890..11068)
                     /gene="hflX"
                     /locus_tag="ACFMWZ_000468"
     CDS             complement(9890..11068)
                     /gene="hflX"
                     /locus_tag="ACFMWZ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC5934474.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11073..12353)
                     /locus_tag="ACFMWZ_000469"
     CDS             complement(11073..12353)
                     /locus_tag="ACFMWZ_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC5934475.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            12355..12483
                     /locus_tag="ACFMWZ_000470"
     CDS             12355..12483
                     /locus_tag="ACFMWZ_000470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934476.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(12506..13231)
                     /locus_tag="ACFMWZ_000471"
     CDS             complement(12506..13231)
                     /locus_tag="ACFMWZ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC5934477.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13304..13981)
                     /gene="radC"
                     /locus_tag="ACFMWZ_000472"
     CDS             complement(13304..13981)
                     /gene="radC"
                     /locus_tag="ACFMWZ_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC5934478.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14104..15453)
                     /gene="gshA"
                     /locus_tag="ACFMWZ_000473"
     CDS             complement(14104..15453)
                     /gene="gshA"
                     /locus_tag="ACFMWZ_000473"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC5934479.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15713..17122
                     /gene="leuC"
                     /locus_tag="ACFMWZ_000474"
     CDS             15713..17122
                     /gene="leuC"
                     /locus_tag="ACFMWZ_000474"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC5934480.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17219..17473
                     /locus_tag="ACFMWZ_000475"
     CDS             17219..17473
                     /locus_tag="ACFMWZ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934481.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGRKIENATE"
     gene            17535..18176
                     /gene="leuD"
                     /locus_tag="ACFMWZ_000476"
     CDS             17535..18176
                     /gene="leuD"
                     /locus_tag="ACFMWZ_000476"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC5934482.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18351..19621
                     /locus_tag="ACFMWZ_000477"
                     /pseudo
     CDS             18351..19621
                     /locus_tag="ACFMWZ_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            19637..20368
                     /locus_tag="ACFMWZ_000478"
                     /pseudo
     CDS             19637..20368
                     /locus_tag="ACFMWZ_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            20402..21472
                     /gene="leuB"
                     /locus_tag="ACFMWZ_000479"
     CDS             20402..21472
                     /gene="leuB"
                     /locus_tag="ACFMWZ_000479"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC5934483.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21635..22198
                     /locus_tag="ACFMWZ_000480"
     CDS             21635..22198
                     /locus_tag="ACFMWZ_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC5934484.1"
                     /translation="MKKIIIAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTSIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5381 a         5887 c         5795 g         5263 t
ORIGIN      
        1 catttccaaa gggcatttta agccgtattt gagagaatgc ggatggcgtt ttcagtgttt
       61 tcagcgtgat aaaattcaaa tttctatttt aaaacaatca gcaaagcagg atttatttca
      121 gttgtccgag acaacgggcg gctgcaccgc ctgcactccc catacttttt caatgcactc
      181 gagcaggagt gagacgtggg gattatcctc gtattcttcc gcataaatga aattgagcgg
      241 catactgtgg cggcacgatt cgataatagc cacgcccgcg ccttcttttg ccgccgcttc
      301 ggctttgttt cccggcacca ttgccacacc tataccgcct gcaaccaaat cgataatggt
      361 ttgggggtag tcgcacaaga tctgtttttt gggtgagagc cggttgctgc gccaaaactg
      421 gtgcaggtgc ttcctacttc cggacacgcc cgacatttct atccatacgc attcttgcag
      481 gctctgcgga agggagcggg tcagatgggg atattggctt tgcgggcaaa tcagcgcgta
      541 ggtcaggttt tgcaggaata cgctgcggat gccgcgttgg gcggcgttgc cgagtataaa
      601 gccgccgtgc agggttttgt gttggatgcg cgagaggatt tcgccgctca ttccgtattg
      661 gatgtgcagg cgcgttttgg gggctgtttg accgatattg tccgtcagcg cgacgagttt
      721 tgccgaatcg atggggtgga taatgcccaa actggtctct tccgaataat cgcctgccag
      781 cgttttggca aaatgctcca gcttgtgttt gtattgcagc agggattccg cttcgggcaa
      841 cagtatttcg cccgcccgcg tcaataccat gcctttcccc gtgcgcctga acagcggcgt
      901 gccgacatat tcttcaaggg ctttaatttg ggcagaaacg gcaggctggg aaaggaaaag
      961 tcgtttggcg gcttgggtaa ggttgccctc gtgcgcgacg gcgacaaatg attttaattg
     1021 tacggcatcc atatatccct ccttgtgcgg atgttttcta tatttgtgca atcgaaatct
     1081 tttaggtgga ttgttgctga aaattaactt tttaatcaag tggtttgtaa attgtatcag
     1141 ttttccggat gatggttatc aaaaaaaaag attggtttta ttgcctgttt gggctttaaa
     1201 tggggttacg gcttccgaac gcagcccgta tcaaaaagaa aagtcatgcg ccccttttac
     1261 gaggcgcgat atataaggag gaaggttatg gaaaaacata atgggactta tcgggatttg
     1321 caccgtccag cttcggaatt tgcgacgcgg gacgaatatt tggaacatga attgcagatt
     1381 atgcaaccaa aacgctggcg gcccaacctg ccctttcgcg attaccgctt cgagtgggag
     1441 gatttgattc ctgcgatggc gggaacgatt ggaaaagtgg tgatggtggg ggcggtggcg
     1501 gcggcgtttg ccgcaccttt ggggctgcct gacagctttg tactggaaaa tgtgcgctat
     1561 gagcttttaa tcgccgccgc gtttatctta ttggtatcgg gcttttttct gcccggcgcc
     1621 aacctgcccg gtacgcacgg gccgctgatt ccgatgattc ccatcgttgt gtcggcaggc
     1681 gggcatcctt tggcgttcgg catttcgatt gcggttttag gtctgctgat ggctttattt
     1741 cgcggcggca gtattatggc gaagctgaca agcaacggcg tatgcggcgg attattactc
     1801 tatttgggct ttatcggcac gacggggcag gtaaaaaaat tgttttcgtg ggcaggcggt
     1861 tttaatatgc cctacatcgc ttttaccgtc attattgtaa cgattgtgat gtacgctttg
     1921 ttggagcatt ggaaaaaacg ctggttagcc gtgcctttgg gatgcttgat tgccggtgtg
     1981 gtggcatttg cattgggtgc gccgtttgag tttcacaccg cccccggcct gcctccaatg
     2041 agtcctgctt attggtgggg tgaaaacagc ggctggcatc tggggttgcc gacggcagaa
     2101 agttttttgg ttgtctttcc atttgcggta ttggctgttg caatgtggtc gcccgatttt
     2161 ttaggacatc aagtgttcca aaaaatcagc tatccggaaa aaaccgataa ggtattgatg
     2221 aatatagacg acaccatgac aagttgttct gtccgtcaag cagtgggttc tattttaggg
     2281 ggtgcaaatt ttacctcttc ttggggaact tatatcgtac cggcatcgat tgccaaacgc
     2341 cccattccgg gcggtgcggt tttaacggcg gttttatgta ttatcgccgg gttatggggc
     2401 tatccgatgg acttggcgat ttggcagccg gtattgagcg tagccttggt cgtaggcgta
     2461 tacttaccgc ttttggaagc gggcatggaa atgacgcgca aaggcaaaac cacccaatcc
     2521 gccgccatcg tggtgttctc ttccgccttg gtcaatccgg ttttcggctg ggcgttgacg
     2581 atgctgttgg ataatttggg cttaatcggc tgcaaagagc gcagtgcgca attaggtttc
     2641 gccggacgcg tgttgatacc cgcagtaggt ttcttgatct tgtgtgtggc gatgggtgcg
     2701 gtcgggatgc tgcccggtat cccgccgttt ttggaacact tcaaatcttt gggctaggct
     2761 gaaatcggaa atgccgtctg aaccgctttc agacggcatt tttgcaaaca ggcaaaatga
     2821 cggcggcggg attttttatt ttcccgattg aagtataatg ttgccgggct tcaaccggat
     2881 attcaaaacg gtttgttcca acactcggaa cggcgcataa aacgccgccc ttcgcgttat
     2941 cccgaacggg gcggctaatc agattctatc gccataaaag gcggggtttc aaccgaaaag
     3001 gaattgagat gaataaaacc ttgtctattt tgccggtggc aatcttactc ggcggctgcg
     3061 ccgccggagg caacacattc ggcagcttag acggtggcac aggcatgggc ggcagcatcg
     3121 tcaaaatggc ggtcgggagc caatgccgtg cggaattgga caaacgcagc gaatggcgtt
     3181 tgaccgcgct ggcgatgagt gccgaaaaac aggcggagtg ggaaaacaag atttgcgctt
     3241 gcgtcgccca agaagcaccc gaacggatga ccggcaacga tgtgatgcag atgctggctc
     3301 cgtccacgcg caatcaggca cttgccgccc tgaccgccaa aacggtttcc gcctgcttca
     3361 aacacctgta ccgctaacct tcttcagacg gcatgaggca gatgccgtct gaagccgttt
     3421 tcctgcaatt tcgacagacg agaaaaatca tgaaatacat cagcacgcgc ggcgaaaccg
     3481 cacacaaacc gttcagcgaa gttttattga tggggcttgc acccgacggc ggcctgatgc
     3541 tgccggaaca ttatccgcaa atcgggcggg aaaccttgga caaatggcgc ggtttggctt
     3601 atcccgaatt ggcgtttgaa attatgcgcc tgttcgttac cgacattccg gaggacgatt
     3661 tgcgcgatat tctgaaccgt acttacacgg aagcggcgtt cggtactaag gaaatcaccc
     3721 ccgtccgcac gctttcagac ggcatcaaaa tccaagcctt gtccaacggc ccgacgctgg
     3781 cgttcaaaga tatggcgatg cagtttttgg gcaatgcgtt tgaatatgtt ttaaacaaag
     3841 aaggcaaaaa actcaatatc ttgggcgcaa ccagcggcga tacgggttcg gctgcggaat
     3901 atgccttgcg cggcaaaaaa ggtgtgaatg tatttatgct gtcgcccgat ggcaaaatga
     3961 gcgcgttcca acgtgcgcag atgtacagcc tgcaagacgg gaatatccac aatatcgccg
     4021 tgaagggtat gtttgacgac tgtcaggata ttgtgaaggc ggtgcaaaac gatgccgcgt
     4081 tcaaggaaaa ataccatatc ggtacggtca attcgattaa ctggggacgc atcgtcgcgc
     4141 aagtggttta ttactttgcc ggctatttca aagcgacgca aagcaatgac gagcaagtca
     4201 gcttttgcgt gccgagcggc aacttcggca acgtttgcgc gggacatatt gccaaacaga
     4261 tgggcttgcc catccgccgc ctgattgtcg cgaccaatga aaacgatgtg ttggacgagt
     4321 ttttcaaaac cggcgcgtac cgcccgcgca acagcgcgca tacttatgtt acctccagcc
     4381 catctatgga catttccaaa gcgtccaatt tcgagcgttt cgtgttcgac ctgatggatc
     4441 gcgatcctca ggaaatcaat acgctgtgga cggaagtcgc ggcgggcaag ggctttgact
     4501 tgcagtttgc cttagacaaa gtcggcggca aatacggctt tacctccagc aagtccaccc
     4561 acgccgaccg cctcgccacc atcaaacagg tttacgagca agaccaagaa ctcatcgacc
     4621 cgcacactgc cgacggcgta aaagtcgccc gcgaagtgcg cgaagaaggg gaaacggtgg
     4681 tttgtttgga aaccgcgttg gcagcgaaat tcgatgcgac catacgcgaa gccgtcggcg
     4741 atgccgccat tccgcgccct gccgcgctgg aaggtttgga aaacctgccg cagcgcgtgc
     4801 aaaccgtgcc gaacagtgcg gatgcggtaa aaggcatcat cgaacaaacc cttgcctgat
     4861 gttccgtaca ggggcactcc gtccgaactt aagattgaaa gaaaatgacc ttacctatgc
     4921 aggaaacccg tttttccata ctgctagacg aattggcggc aaaacagaag gcagccatcg
     4981 ccccccacct gcttgccgac gggagcaagg tatggatacg caaagcaggc aggcacaatg
     5041 cgcgatggcg ttacgcgctg ctcggtatgg ttgcccgata tttgaaactg ggggtgttga
     5101 agccggttcc cagcctcggc ggcgagcctg ccattgcaac cgaatcaaaa cgcctgtacg
     5161 aattgcgcgc ggcgaggata gccgttcccg aattgctcgc cgtcaggaaa aatgccctta
     5221 tgttcggcaa tttggaaggc atcccgctcg atacgcaaat ccgccaagaa gccgaagccg
     5281 gaaaggcgga cgcttggctt gccggtttgg aagccattgc acgcgtgcat aaaaaacagc
     5341 agttcctcag tcaggcgttt gcgcggaata tgatgtcgga cggaaaaaac atcagctttc
     5401 tagattttga agacgaccct tccgaagtcc tgaccatagc gcaatgccaa gcccgcgatt
     5461 ggctctgcta catccattcg accgcgctga tattgaaaaa cggcggactg ctggaagcgg
     5521 ctgcggaaaa atggggcggc gtattgtcgg atcagcctgc cgaaatacaa aagctgattg
     5581 ccgccaccgt caaaccgatc ctcccgatac gcaggctgga acacccacgc tggggacggg
     5641 acgcactcag gctggcagcc tcgatttccc tgatttccct tgccgatatg ccgccataag
     5701 ccgccgcccg ccccgcgatg ccgtctgaag cccttcagac ggcatataac caacgcgcat
     5761 caagcattgt cgcaaccatc cgtttcgttt agaattttgg ctttaccccg aatcgggcag
     5821 acattttcac tctgccgcac atcccgaaca agcactatcc atttctttta ctgacaggaa
     5881 acccaaatgg cagcattcaa tacccaaaaa gtattgtccg tacaccactg gacagacgca
     5941 tattttacct ttacctgcac ccgcgacgaa tcgttgcgct ttgaaaacgg acagttcgtt
     6001 atggtcggat tgatggtgga cggcaagccg ctgatgcgtg catacagcgt cgcctccgcc
     6061 aactgggaag aacacctcga atttttcagc attaaagtcc aagacggccc gctgaccagc
     6121 cgcctgcaac acctcaaagt cggcgacgac gtgttaatca gcaaaaaacc gaccggaact
     6181 ctggttgccg gcgacctgaa tccaggcaaa cacctttact tgttgagcac cggtaccggc
     6241 atcgcccctt tcttgagcat caccaaagac cccgagattt acgagcaatt tgaaaaaatc
     6301 atcctcgtac acggcgtgcg ctacaaaaaa gatttggcgt actacgaccg ctttaccaaa
     6361 gaattgcccg aacacgaata cctcggtgac ttggttaaag aaaaactgat ttactacccg
     6421 attgtttccc gagaagaatt cgaacaccac ggccgcctaa ccgacctgat ggtgagcggc
     6481 aaactgtttg aagacatcgg cctgcccaaa atcaacccgc aagacgaccg cgcgatgctg
     6541 tgcggcagcc ccgcgatgct gaaagacacc tgcaaagttt tggacgattt cggtctgacc
     6601 gtctccccga aaacaggcgt gcgcggcgac tacctgattg agcgtgcgtt tgtagatcaa
     6661 taagctgttt gaaaatcccg tctgaacggc atcagcgata aaaaaacccg aatcctgcca
     6721 atcaggattc gggtttttat agtgggtaaa aattagaaat gcacatttcc gtcatttccg
     6781 cgcaggctag gatccaaact tatccgtttc attatagtgg attaacaaaa accagtacag
     6841 cggtgcctcg ccttgccgta ctatttgtac tgtttacggc ttcgtcgcct tgtcctgatt
     6901 tttgttaatc cactatagta tctgaaaata aaagagaaac caaacatata gattcccgct
     6961 tgcgcgggac tgacgacggt gttctgcgta gccaaagcag aaaaacatcc ttattgtcat
     7021 ttcatcatga tataacaata aggatgttaa aaattagtta tcattatatg catgatatat
     7081 aagaaaaatt gtcccaataa gaaatgttat gcagaaaata aaatgaaaag gatggttttc
     7141 attgaaaatg gttgttctgc caccgtcata cacagaaccg gtatttggaa aaaaaataat
     7201 ccatattgac atcattattc catttatcgc ataaatccac tttttaattt ttttcctcat
     7261 taatataatc tcttatttaa taaaatcaaa tacctagcaa ttcgatctca aattttaaac
     7321 gccaacgtgt ttgagcggta acaatgcata ctttaagttt gtaatccttc atcgtctcat
     7381 ttttgtttta atcgactata cgtctaaagc aacgccgtcc gtcattgttc ggacggcatt
     7441 ttttcagacg gcattttccc ccgtttgtga aggcgcaagg cattgcccaa aaccgccagc
     7501 gaactgaaac tcatacccag tgcggctatc cacggttgga catagcccaa aacggcaagc
     7561 ggtacggcaa tgatattgta cgcgcccgcc catatcaggt tttgccggat aatctgccgg
     7621 gtgcgccgcg cctgatcgag caggtgggcg acggtacgca aatcttcgtt caataacaca
     7681 atgtccgcgc cgtccctcgc aatatccgtc ccgcccgctg cggcggcgga tacgtctgcc
     7741 tgcgccaaaa cgggcgcgtc gttgatgccg tcgcctatca tcagtacttt tttcccttct
     7801 ttttgcaagg ctttgacgta ttccagtttg tcctcgggca tggcttgggc gcggtagtgc
     7861 gtgataccca gggcgcgggc ggtttcggca acggcggttt cgcggtcgcc gctgaggatg
     7921 tgcagggtca ggtttttgcc tgccaactgc cgcaccgcct ccgccgcgct gtctttcaag
     7981 gggtcggtca ggtagaacac ggcttggaaa ccgctttgac tgccgaggta aaccgcgctg
     8041 ccgccgcctt ctgtttgcgg ttctttacct gaaatttcgg cgacatagga tgccctgccc
     8101 aatgcccaaa cctgtgtttc gccgttgacg gtcagttgcg cgcccacgcc ttcgccgatg
     8161 cggttgaggc gttgtttaat agcgatgtcg gggacgctgc cgtctgaaat gcggcagttg
     8221 aggatggcgc gggcaagggg atgttcggac tgttgttcta aagcctgcgc caccgcgaga
     8281 acaaaggctt cgtctgtgcc tctcaacaat gagatacggc ggacggcggg tttgccttgg
     8341 gtcagcgtgc cggttttgtc gaagatgatg tcggtggttt gggcgagggt ttcgattgcc
     8401 tgctttccgc cgattaaaat accttcgcgc gccagcgtac cggtagaagc tgccagcgcg
     8461 gtcggcgtgg caagcgataa ggcgcagggg caggtaatga ccagcagggc gacggtaatc
     8521 cacaatgcgg tgtgcgcgtc ggcgtacagc gtccagccga tgaagacggg gacggcaagc
     8581 aggagttcgc cgaatatgaa agacgaggcg tattgttccg ccaactcggc agtgcgcggt
     8641 ttttgcgcta aggcgcggtc gagcaggcgg acgatgtgcg acagtcgcgt gccgccgccg
     8701 gtgcggtcgg tgcgtataat cagggggctt tgcgtgttga gtgtgccggc ggttactttt
     8761 tcagacggca ttttggcgac gggcaggctc tcgccggtca gcatagattc gttgacggca
     8821 ctgcttcctt ccagcaccgt gccctcaacg gggatggttt cgcccggttt gaccagcacg
     8881 atatcgcccg ccttcaattt gacgacagct gcctcgcagg tttcctgcgt atcggggtaa
     8941 tcgggcatat gatggcaaaa cgcaggaatc agcttcacca gcctctcggc ggcatcgcct
     9001 gccttacggc gggcaatgtg ttccataaag cgtccgccca gcaggaaaaa cagcagcatc
     9061 gcgatggatt cgaaatacat cccctgcccc gcatttgtcg caaggctgta aacgccggcg
     9121 ataaaggtca tgatgatggc gacggcaatc ggcgtatcca tgccggcgcg gcggtttttc
     9181 aggtcgcgca acgcgccttg ataaaacggg acggcgcaat agaatacgac gggcagcacc
     9241 attaaaaagc cgccccaatg gaggatttgc aggaaatcgg gttcgatgtc gccgccgtaa
     9301 aggtaggtcg gcagcgcgaa catcatcgtc tgcatcatcc ccagcccggc aacggcgagg
     9361 cggacgatgt attgttttcg ttctttttgg ttggcggctt cgattttttg cgcgtcatag
     9421 ggtgcggcgg tgtagcctat ctgcctgatt ttcaacagaa tgtcggaaag gcggattttg
     9481 ccgtcgtccc agacgacgcg gcagcggtgc gtgctgtaat tgaggtcgat gcggacgatg
     9541 ccgtctgtac gcaaaagctg ctgttcgatc agccagacgc aggcggcgca ggtgatgccg
     9601 ccgagcatta aaaccgcctc gcgcgtgccg ccgtgggttt ccacaaagtc ggactggact
     9661 tcgggcaggt cgtacaggcg gatttggtcg aggatttctt ggggcggcag ctcggttttt
     9721 tgcgcgtcgg cggtgcgttg tttgtaataa ctgcccaagc ccgcgtcaat aatgctttgt
     9781 gcgaccgcct gacagccggc gcagcaggtt tcgcggtctt cgttttcgta acggacagtc
     9841 aggtggaggt gttcgggaac atccagaccg cagtggaaac aggttttttt catggcattt
     9901 cggtttcgtc tgtgtttggt gcggcggcac aagactcggc aatggcttcg cgcagtgcgt
     9961 ctataccggt attttcagca acggaaatgc ggacggcggc aatttttccc gcagcgtcgc
    10021 gccatatgcc cgtgttttgt tcttcagacg gcagcaggtc ggttttgttg tacaccttga
    10081 tgcacggaat atcgccggca tggatttccc gcagtacgtt ttccacgtct tcaatctgct
    10141 gtccgctgtt cggggcggcg gcatcgacga cgtgcagcag cacatcggct tgcgcggttt
    10201 cttccagcgt ggcggaaaat gccgaaatca gtttgtgcgg caaatcactg acgaatccga
    10261 cggtatcggt caggataatg ctgcattcgg gactgatgta cagccgccgc gccgtggtgt
    10321 cgagtgtggc gaaaagctgg tctttcgcat atatgcccga cttggtcagc cggttgaaca
    10381 ggctggattt gccgacattg gtatagccga ccagcgcaaa cgttttgatt gtgcccgatt
    10441 cgcggggctt gcggcgcagg gcgcgctgtt ttttgaggtt gacaagctgt tttttcaagg
    10501 cattgatccg atgggcgatc aatcggcggt cggtttccag tttggtttcg ccggggcctt
    10561 tcatgccgat accgccgcgc tggctctgca gatggccgta accgcgtatc aagcgtcccg
    10621 ccaaatggct caattgcgcc aactcgactt gcagcctgcc ttcctgcgtg cgggcgcggc
    10681 gggcgaaaat cgccagaatc agccctaccc tgtccaatac gcggcatttc agttcttttt
    10741 caaggttgcg ttcctgcgtg ggcgtgagtt cgtggttgaa tacgaccaaa tcgatgccgt
    10801 ctgcggcaac tgcttctgac agctccgccg ccttgcccgt gccgacaaac agcgcggtgt
    10861 gcggacggtc gcgtttggca gtctccacgc gcacggaatc gccgcccgcc gctttgacca
    10921 gctcgacagc ttccgccaat gccgtctgaa aaccgttcag acgggtggca ctgctgcccg
    10981 tgccatcttt gtccaacatt acgcccacca gcatgacgcg ttcgggttgc gcttgggtac
    11041 tgccgttgcg gcctgttctg cctgtcatgt attcaatcct gtaaataaag ggttacgcct
    11101 cgttccgttt ctccttccgg tgcaagcgtg atgcggctgc ccgccattcc gtcaacgctg
    11161 aagacgatgc cgcccgcctc gtgccttcct atggacaggc ggctgccggg tcgctgccgg
    11221 taggtataac gggtacacgc ttccaaacac cagcgtccgc gccagtggat gaaatatttt
    11281 tcgccttttc taaccgcaag tgtctcgccg ccgtttacct caaaataagg gtaatcgggt
    11341 tttcgggttc taaactgcca tttcgcctgc gcgcgccgtc cgttgcggac atagtcgaat
    11401 accgccgtat aaagcgtacc gtattccaaa ggcttgagcg ggaaaagcgc gaattggtac
    11461 gcggtcagcc tgccgttggg gtcgttgccg gcggttaaaa ccctgacggg gcggatttcg
    11521 tttttaccct gatacagctt gaaacttttc atcgtaattt tgcctgccgc ctcggaaaaa
    11581 tcaatgctgg caggattgcc cgtgatttca tattccggca cggggtctgg acgctcgccg
    11641 tggaaataag gcagtgcgcc gttgccgacg ggataggctg tatagagcag ctcctgtgcg
    11701 ggcatggctt cgtcggtgta cacgaccgca ccgttatggc aggcgttgcg gtaatatttc
    11761 cgtcctgctt ccggctgatt tctgccttgg gcgcaatgcc gctcaaacct gccgttgccc
    11821 tgattgaata cgagaacggt tttaccgttt tcgcgcacaa atgccgctcc tgcctcatcc
    11881 gtatggcggt caagtaggga aaggcggtgg tagattgcgc tcattaatcc gtccacttgg
    11941 cgttgctgcg tgcggatgtc gctgtcggac gattcggcgg cttcctcttc cgtgctgatg
    12001 ttttcatgca cgccgttgta gagatacccg gcaaggcgtg tgcgttcggt cagcttttgt
    12061 gcggtgtagt gcggattgtc gggatggtgt tcgccgtgtc cgtcttcggg attgagcgtg
    12121 agatagcgtg cgtgcctgcg ggcggaattt tccaaaaccg gcgcgtgtgc cagcttgtgc
    12181 aaaccgattt gggcgcggat gcggttgagg tagtgcagcg catcgccgcc gtccctgatt
    12241 tgcggtgcgg acggatagac aagttcgccc gcgggcaggg attggttttg ggtatgatag
    12301 aaaacgccgg ccgccgaacc caacaggagc agccgaataa aaagggattt cataatgccg
    12361 tcctcccgac gcggcaacgg ggtgttttat caaaacggca aacttgccaa tgcggtttcc
    12421 gattgcagat tgccaaaccg gcaaaccttt ccccgtgccg atgccgaacc cgatgccgtc
    12481 tgaaccttca gacggcatcg ggtgtttatt tgtccacccg gatgggtgca ggttcgtatt
    12541 gtgtcgattc gtcgccgtaa tacagcacgc cgagtttgat ggggatgcgt ccctgcgatt
    12601 tgcggtgggc gttggaatcc ctcagggaat aggcacaacc gcaatattcc tgctggtaga
    12661 agttttcacg tttgctgatt tcaatcatac gcgcgctgcc gccgcctttg cgccagttga
    12721 aatcccaata caccacatca tcgtaaggcg cggcggcgcg gtgtccgcag tcgttgattt
    12781 gcgccatatt tttccagcgt gaaatgccca gcgaactggt aaagacgggg aacccgtgtt
    12841 catgcgcgta ttgcgccgcc ttttcaaaac gcatatcgaa acacatggtg cagcggatgc
    12901 cgcgttccgg ctcaaactcc atgcctttgg ctttggcaaa ccattctttg cggtcgtttt
    12961 cgtagtcgtc gtctttatcg atgaaaggaa tgccgaactt ttccgcaaag cgcacgtttt
    13021 cctcttttcg gagcatatac tctttgtgcg gatggatatt gggattgtaa aaataaatgg
    13081 tgtagtcgat gccggaggca agcatcgcct ccatcacttc gccgctgcac ggggcgcaac
    13141 aggaatgcag caagactttt ttatgtccgc cgggcgggac gagtatgggg cggtcaatgt
    13201 cggtaacggt aggtttgttt tgtgtttcca ttaacttaac gatatctgtc gaatacggtt
    13261 gaagcggcta ttttatcaga gccgccgcat gtaaaacagg gtgtcagggc atcagcccga
    13321 gctgcctgaa cgaacggacg gtttgcgccg ttacgataaa atggtcgagc agcgacacat
    13381 cgaccagcga cattgcctgt gccagccgcc ttgtgaacat gatgtcttcc tgcgaaggtt
    13441 cgggcgagcc gcccggatgg ttgtgcgcga taatcaggct gtcggcatat tcgtccaatg
    13501 ccagtttgac aatttcacgg atgtaaatcg tgttttccgc aaccgtaccg cgcgacagct
    13561 ctctgaccgc aatcagttgg ttttggcggt tcaacagcag cgcgacgctg acttcgactt
    13621 tttcctgccc caaatgaaag cgtaaataat cggcaacggt atccggatcg gacaggacga
    13681 tgctctcctg caattcttcc gccaatatcc gccgcccgat ttccctgacc acggcgaact
    13741 gtgtgaaact tgccgtcccc atccctttgt atgccgacag tttgccgacc tccgcgctca
    13801 tcagcctccc caaactgccg aactcctgca gcaaataacg cgccaaatcg accgcactca
    13861 ttccgcgcgt gccgacgcgc agcaggattg ccaaaagttc ggcatcactc aaagccgccg
    13921 ccccgcgttc caacagcttt tccctgggcc tttccccctc cggccattgc ttgatgctca
    13981 tgttttccct tttttaaata gtctgagatg ccgtctggaa caaatgttcc cacacgccaa
    14041 atatccgaaa cgccgtctga accggatttc cgcttcagac ggcatctgta tttcaaaaca
    14101 gtcttacgcc gtttcttcca actcgatgga cgccgccaga agcgacaggc gtgccattac
    14161 gccgtaaacg tagaggcggt tgtgttcgtt gtccacgtcg cagtcttttt cagagcgcgg
    14221 catacccagc gagtcccatt gttcgaatac gcgcttgcag gcttcgggag cttcttggga
    14281 gttgtcgccg ttgccggtgg ggatgctgtt ggacagcgga acaaacacca taccgccggc
    14341 gttgaggttt tcgtccgcac cgcgcccttc gtgtacgcgg aaaaagccgc cgatgacgaa
    14401 gcggtccatc atatacacga cgggttcgca caccgcgccg tttaaggttt cataagtata
    14461 aatcccttct tggacaatca cttcgctgac ttccaaacct tctttgactt tcgccatttt
    14521 attgcggttt ttacggttca atccgcgcac ttcgtcggcg gatttgacgc tcatcacgcc
    14581 catgccgtaa gtgccggcat cggctttgac gatgacgaaa ggtttgtcgg taatgcccga
    14641 ttcgtcgtat ttggcttgaa ttttcgccag cacacgttct accgcttccg ccaacgcgtc
    14701 ttcgccttca cgcccttgga agtccaaacc gccgattttt tcaaaataag ggttaatttg
    14761 ccattcgtcg atgccgatta acttggcaaa ttcggcggca acttggttgt acgcgccgaa
    14821 atgatttgtt ttgcggcgcg tcgtccaacc gccgtgcaac ggcggcaaaa cggtttggct
    14881 gatgcctttg aggatgtcgg gaatgccggc ggacaaatcg ttgttcaaca aaaccacgca
    14941 aggcgaaaag ccgtctgcaa gatggacgcg gtcgcgggta cgcaataaag gttccaacag
    15001 gattttgtcg cccaatgcgg tttcaaattc ggtcggttcg gttacttccg gattcaggct
    15061 gcccaagcgc acttcatacc ctgccgaacg caaaatctcg ccgagggcgt aaatgttttg
    15121 caggtaaaac gtattgcgcg tgtggttttc gggaataatc aatacggatt tcgccgtttc
    15181 gcaggcgcgt tgcaccgcat cttgcgcggc aaccgccgcc agcgggataa agttgggatt
    15241 cagattattg aagccgccgg ggaacaaatt catatcgata gacgaaattt tgtaaccggc
    15301 attgcgtata tcgaccgaac cgtaaaacgg cgggcggtgc gcgttccact gcgtgcggaa
    15361 ccacgcctcg attttggcgt gattggacag gatttttgcc tcaaacgcct gaagttgcgc
    15421 cgaatgttcg ggcgacatca ccggtaattt catctttcgt ctgcctctgt gtgttttttg
    15481 acaagatgca gatgataatg cctttgaaag aatgacgaca agtattcttt cgtatcgaat
    15541 catgctttag atataactgt ttactgaaat ggacaatatg tctaattttt tcgttaaaat
    15601 tgtttgcaaa ataacaccgg ctcaaaaatt agacaaaatt tgttgcgcgg tataaagaat
    15661 acgtctaaaa tccgcccaat ccgcattacc atttatccaa aagaacaaca tcatgacagc
    15721 acaaaccctc tacgacaaac tttggaacag ccacgtcgtc cgcgaagaag aagacggcac
    15781 cgtcctactc tacatcgacc gccatttggt gcacgaagtt accagccctc aggcatttga
    15841 aggcttgaaa atggccggcc gcaaactctg gcgtatcgac agcgtggttt ccaccgccga
    15901 ccacaacacc ccgaccggcg attgggacaa aggcatccaa gacccgattt ccaagctgca
    15961 agtcgatact ttggacaaaa acattaaaga gtttggcgca ctcgcctact ttccgtttat
    16021 ggataaaggc caaggcatcg tacacgttat gggcccggaa caaggtgcta ccctgcccgg
    16081 catgaccgtt gtctgcggcg actcgcacac ttccacccac ggcgcattcg gcgcactggc
    16141 acacggcatc ggcacttccg aagtcgagca caccatggcg acccaatgta ttaccgcgaa
    16201 aaaatccaaa tccatgctga ttgccgttga cggcaaatta aaagcgggcg ttaccgccaa
    16261 agacgtggca ctttacatca tcgggcaaat cggcacggcg ggcggtacag gctacgccat
    16321 cgagtttggc ggcgaagcca tccgcagcct ttctatggaa ggccgcatga ctttatgcaa
    16381 tatggcgatc gaagccggtg cgcgctcagg catggttgcc gtcgaccaaa ccaccatcga
    16441 ctatgtaaaa gataaaccct tcgcacccga aggcgaagcg tgggacaaag ccgtcgagta
    16501 ctggcgcacg ctggtgtctg acgaaggtgc ggtattcgac aaagaatacc gtttcaacgc
    16561 cgaagacatc gaaccgcaag tcacttgggg tacctcgcct gaaatggttt tagacatcag
    16621 cagcaaagtg ccgaatcctg ccgaagaaac cgatccggtc aaacgcagcg gtatggaacg
    16681 cgcccttgaa tacatgggct tggaagccgg tacgccatta aacgaaatcc ccgtcgacat
    16741 cgtattcatc ggctcttgca ccaacagccg catcgaagac ttgcgcgaag ccgccgccat
    16801 cgccaaagac cgcaaaaaag ccgccaacgt acagcgcgtg ttaatcgtcc ccggctccgg
    16861 tttggttaaa gaacaagccg aaaaagaagg cttggacaaa attttcatcg aagccggttt
    16921 tgaatggcgc gaaccgggct gttcgatgtg tctcgccatg aacgccgacc gcctgacccc
    16981 ggggcaacgc tgcgcctcca cctccaaccg taactttgaa ggccgtcaag gcaacggcgg
    17041 acgtacccac ctcgtcagcc ccgctatggc agcagcagcc gcagtgaccg gccgctttac
    17101 cgacatccgc atgatggcgt aagtattttc aggcggtctt taagaatttg acgcggccgt
    17161 ctgaaatatg tcccgtaagg cagaaacacc tttaatattc aaaccaagga gtttaaccat
    17221 gaacaaactt ttcattaccg ccctgtccgc cctcgccttg tccgcctgcg ccggcacttg
    17281 ggagggcgcg aaacaagaca ccgcccgcaa ccttgacaaa acacaggccg ccgccgaacg
    17341 cgccgccgaa caaacaggca acgccgtcga aaaaggctgg gacaaaacca aagaagccgt
    17401 caaaaaaggc ggcaatgccg tcggacgcgg catttcccat ctcggcagaa aaatcgaaaa
    17461 cgccaccgaa taaccgattc gcgatgccgt ctgaaaccgc ttcagacggc atcgtcagca
    17521 caagacgcaa acccatgaaa gcctttacca aaattaccgc catcgtcgcc ccgctcgacc
    17581 gcagcaacgt cgataccgac gccatcatcc ccaaacaatt tttaaaatcc atcaaacgca
    17641 gcggcttcgg ccccaatgcc tttgacgaat ggcgttacct cgaccacggc gaaccaggca
    17701 tggacaacag caaacgcccg ttgaaccctg atttctcgct gaaccagccg cgttaccaag
    17761 gcgcgcaaat cctgttgacg cgtaaaaact tcggttgcgg ctcttcacgc gaacacgccc
    17821 cttgggcatt ggacgactac ggcttccgcg ccgtcatcgc ccccagcttc gccgacatct
    17881 tctttaacaa ctgctacaaa aacggccttt tacctatcgt gttgaccgaa gaacaggttg
    17941 accggctttt caaagaagtt gaagccaacg aaggctatca gctctccatc gaccttgccg
    18001 aacaaaccct gaccacccct ggcggcgaaa cattcacatt cgacattacc gaacaccgca
    18061 aacactgcct cttaaacggc ttggacgaaa tcggactgac cctgcaacat gccgatgaaa
    18121 ttcacgcctt tgaagaaaaa cgccgccaaa gccagccttg gctgtttaac ggttaaaacc
    18181 tatgccgtct gaaggccgtt cagacggcat tcctgataac atataattgt tgcggctaat
    18241 gtaaacagag gggcgataaa ttgctttcat cttttaagcg ggcttagcga cactctaaat
    18301 taatgcaata atgcaactct ccatttcaaa taaattttta gatttttaat atgcaacaaa
    18361 ttaaatttat tgacttattt tccggaatga gtggcatcag gaaaggattc gaacaagcct
    18421 gtcggaaaca gtcggttgct tgcgaatgtg tttttacttc agaaatcaaa ccggcagctt
    18481 tggaagtatt gaagcaaaac taccctgatg aagtgccgta tggagatata acgaaaattg
    18541 aaacagggga tattcccgat tttgacatcc tgttggcagg cttcccttgt caggcttttt
    18601 ctttcgccgg aaaaagatta ggctttgaag atacacgggg aacgcttttc tttgatgtgg
    18661 caaggatttt aaaggcaaaa aaaccaaaag gttttatttt ggaaaatgtg gaaggattgg
    18721 tgacacacga tagaaaagat tcgacacaaa aaatagggcg caccctgacc gttattttgg
    18781 aaaccttgga agcattgggc tactatgttt cttggaaagt tttaaatgca aaagatttcg
    18841 gcattcccca aaacaggaag cgcatttatc tgacaggcag tttgaaatcc aaacccgact
    18901 tatctttcga aacaagccca agtccgaaat taaaaaatat tttggaatcg ggactgccta
    18961 ccgaaagcag ccctttcatc aaaaaattgc taaaaaaatt tcccccgtcc gaactgtacg
    19021 gaaaatcagt aaaagacaaa cggggaggga aaaacaatat tcacagttgg gatattgaat
    19081 taaaaggcgc agtaaccgaa gaagagaagc aattgttaaa tatccttcta aaagaacgga
    19141 ggaaaaaaaa tgggcttcag aaatcggcat agattggatg gatgggatgc ctttgacaaa
    19201 agcgcaaatt tcaactttct ataaacaccc cgatctccaa aatattttgg acagcctgac
    19261 agataaaggc tatttggttt tagagcatcc gaaacaaaaa attggcgggc aaagaatcaa
    19321 agatgaatcc ctgcccaaag gctacaatat tgtttcaggc aaaaaatctt ttgaaatcaa
    19381 taaaatatta gatcaaaacg atgttgcgcc aaccttggtt gcaatgaata tggagcacct
    19441 tttcgtcgtt gacaacggcg gtttgcgtac acttaccgga aaagaagggt tgcgcttatt
    19501 cggctatccg gacgattatt cgtttgatat tcccaaaaaa gacagatgcg atttattggg
    19561 taataccgtt gccgtccctg tgattaaggc ggtatctgaa agacttctgc atactttata
    19621 aaccgacagg gataaaatga taaaacttac tgcacaacaa atatttgata agttgctgga
    19681 tgaagaaaaa atcttatcgg ccaatggtca aattagattt ttcttaggcg atgtggatat
    19741 tatcgtcaaa caaaaagatg ttgtcggcaa catcattcag gaatggctcg gcggatgatt
    19801 gagaaaaagg gaaattgaat ttgatgtttc aaccaatacc caaatgcctc ccgatttctt
    19861 tttaaataaa aaagaccgca gcagagaatt gttggaagta aaggcgttca accgaaatgc
    19921 cggcccgggt tttgatattg cagattttaa aatgtattct gatgaaatca ttcataagcc
    19981 ctatatgctg gatgtagact atttaatatt cggttacgat atggacgaca acggcaatgt
    20041 aaccatcaag gatttatggc ttaaaaaagt atggcaaatt acgagaagca tggatgggtg
    20101 ggcaattaat cttcaagtca aaaaaggcgt ggtgcataaa atccgcccgg gtgtttggta
    20161 cagcataaat aaaaagaata tgcctatgtt tgaatgcttg gaagattttg tttccgcaat
    20221 tgaagaaacc gtttatcaaa acccggcaac acggcataac gcatccttat ggaaaaagaa
    20281 atttgaggaa gcctataaaa aacactacaa ccgatcgatt tccatacctc gttggcacga
    20341 aattgcacac aaatacaaaa agaaataacc cttcaaccat caacccataa ggaaacttga
    20401 aatgaccaaa catatcgcca tcctccgagg tgacggcatc ggcccagaaa tcgtcgccga
    20461 aaccgtccgc gtactcgaca aacttatcgc ccaaggcttg gatgccggct acgaatacgc
    20521 cccattgggc ggcgaagcct acgacgaata cggccatccc tatcccgaat tcacgcaaaa
    20581 cctctgccgc aaagccgatg ccgtcctgct tggtgcagtc ggttctcctc aatacgacaa
    20641 cctcgaccgt ccgttgcgtc ctgagcgcgg cctgttggca atccgtaagg atttgaacct
    20701 gtttgccaat ttgcgtcctg ccgttttata tcccgaatta gccaacgcct ccacgctgaa
    20761 gcctgaaatc gttgccggtt tggatatttt gattgtgcgc gaactcaccg gcgacatcta
    20821 tttcggcgaa ccgcgcggca tccgcgttct ggaaaacggc gaacgcgaag gctacaacac
    20881 catgaaatac agcgaaagcg aaatccgccg catcgcccac gtcgctttcc aatccgccca
    20941 aaaacgcagc aaaaaagtct gctccgtagg caaagccaac gttttggaaa ccaccgaact
    21001 gtggcgcgaa atctttgaag aaatcggcaa acaatacccc gatgtcgaac tctcccacat
    21061 gtatgtagac aacgccgcca tgcagttggt acgcgcgccc aaacaatttg acgtgattgc
    21121 caccggcaac atcttcggcg atattttatc ggacgaggcc tctatgctga ccggctccat
    21181 cggtatgctg ccttctgctt ctttggacga aaacggcaaa ggcctgtacg aaccgtctca
    21241 cggctccgct cccgacatcg ccggacaaaa caaagccaac ccgctggcca ccatcctctc
    21301 gctcgcaatg ttgctgcgtt acagcctgaa cgacgaagcg cgcgcgcaac aagttgaaaa
    21361 cgccgtccaa aaagtgctgc aacaaggctt gcgtaccagc gacatttacg aagaaggcac
    21421 aaaactcgtt tcctgctccg aaatgggcga tgcggtactc gccgccttgt aaaaaaacaa
    21481 agcgcggatc acaccgcgct ttttcatctg cccttttccg ctcgccccgt tcgtcaaccg
    21541 cctgcaaacc caccgtttac cggcaggaaa tgccataaat ccccgcctat gttaggataa
    21601 acgcctttac tggctcacac aaaggattat catcatgaaa aaaatcatca tcgccgcact
    21661 cgcagcggca gctgtcggca ctgcctccgc cgccacctac aaagtggacg aatatcacgc
    21721 caacgcccgt ttcgccatcg accatttcaa caccagcacc aacgtcggcg gtttttacgg
    21781 tctgaccggt tccgtcgagt tcgaccaagc aaaacgcgac ggtaaaatcg acatcaccat
    21841 ccccgttgcc aacctgcaaa gcggttcgca acactttacc gaccacctga aatcagccga
    21901 catcttcgat gccgcccaat atccggacat ccgcttcgtt tccaccaaat tcaacttcaa
    21961 cggcaaaaaa ctggtttccg ttgacggcaa cctgaccatg cacggcaaaa ccgcccccgt
    22021 caaactcaaa gccgaaaaat tcaactgcta ccaaagcccg atggcgaaaa ccgaagtttg
    22081 cggcggcgac ttcagcacca gcatcgaccg caccaaatgg ggcgtggact acctcgttaa
    22141 cgttggtatg accaaaagcg tccgcatcga catccaaatc gaggcagcca aacaataaat
    22201 cttgatgcga atcagcacaa atgccgtctt catcaaaccg ccccgccaac cgtaagccga
    22261 tatggcggag tacaacccgc cgaaagatat ttcagcggca ggcagggctt gccggaaagt
    22321 ccggaa
//
LOCUS       DAXHKX010000014        22229 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHKX010000014 DAXHKX010000000
VERSION     DAXHKX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22229
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.014"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            18..212
                     /gene="thiS"
                     /locus_tag="ACFMWZ_000481"
     CDS             18..212
                     /gene="thiS"
                     /locus_tag="ACFMWZ_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC5934485.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQQPFAVAVNTVFIPKGAY
                     AETVLNENDKIDIVRPVVGG"
     gene            426..1214
                     /locus_tag="ACFMWZ_000482"
     CDS             426..1214
                     /locus_tag="ACFMWZ_000482"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC5934486.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(1976..2800)
                     /locus_tag="ACFMWZ_000483"
     CDS             complement(1976..2800)
                     /locus_tag="ACFMWZ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC5934487.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADAEEIRKITSLYGKLNLKSCK"
     gene            complement(2913..4691)
                     /locus_tag="ACFMWZ_000484"
     CDS             complement(2913..4691)
                     /locus_tag="ACFMWZ_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC5934488.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKVDGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            complement(4688..5194)
                     /locus_tag="ACFMWZ_000485"
     CDS             complement(4688..5194)
                     /locus_tag="ACFMWZ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFC5934489.1"
                     /translation="MVDAWSVPDFESKICPPEALSERLARLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKDGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            complement(5330..5405)
                     /locus_tag="ACFMWZ_000486"
     tRNA            complement(5330..5405)
                     /locus_tag="ACFMWZ_000486"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5370..5372),aa:His,seq:gtg)
     gene            complement(5433..5509)
                     /locus_tag="ACFMWZ_000487"
     tRNA            complement(5433..5509)
                     /locus_tag="ACFMWZ_000487"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5473..5475),aa:Arg,seq:tct)
     gene            complement(5534..5611)
                     /locus_tag="ACFMWZ_000488"
     tRNA            complement(5534..5611)
                     /locus_tag="ACFMWZ_000488"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5574..5576),aa:Pro,seq:tgg)
     gene            5834..6688
                     /gene="folD"
                     /locus_tag="ACFMWZ_000489"
     CDS             5834..6688
                     /gene="folD"
                     /locus_tag="ACFMWZ_000489"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC5934490.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            complement(6750..7331)
                     /locus_tag="ACFMWZ_000490"
     CDS             complement(6750..7331)
                     /locus_tag="ACFMWZ_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC5934491.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLVGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            7507..8622
                     /gene="asd"
                     /locus_tag="ACFMWZ_000491"
     CDS             7507..8622
                     /gene="asd"
                     /locus_tag="ACFMWZ_000491"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC5934492.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGAETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPISEIEAILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            complement(8715..9245)
                     /locus_tag="ACFMWZ_000492"
     CDS             complement(8715..9245)
                     /locus_tag="ACFMWZ_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC5934493.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(9256..9600)
                     /locus_tag="ACFMWZ_000493"
     CDS             complement(9256..9600)
                     /locus_tag="ACFMWZ_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934494.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            complement(9587..10366)
                     /locus_tag="ACFMWZ_000494"
     CDS             complement(9587..10366)
                     /locus_tag="ACFMWZ_000494"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC5934495.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            complement(10454..11875)
                     /gene="cysS"
                     /locus_tag="ACFMWZ_000495"
     CDS             complement(10454..11875)
                     /gene="cysS"
                     /locus_tag="ACFMWZ_000495"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC5934496.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            complement(12563..13612)
                     /locus_tag="ACFMWZ_000496"
     CDS             complement(12563..13612)
                     /locus_tag="ACFMWZ_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC5934497.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            complement(13695..14849)
                     /gene="obgE"
                     /locus_tag="ACFMWZ_000497"
     CDS             complement(13695..14849)
                     /gene="obgE"
                     /locus_tag="ACFMWZ_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC5934498.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAACPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            complement(15138..16022)
                     /gene="rsmI"
                     /locus_tag="ACFMWZ_000498"
     CDS             complement(15138..16022)
                     /gene="rsmI"
                     /locus_tag="ACFMWZ_000498"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC5934499.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGCLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELLPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNVMKILAAELPTKQAAELAAKITGE
                     GKKALYDFALSCKNKDLI"
     gene            16068..16415
                     /locus_tag="ACFMWZ_000499"
     CDS             16068..16415
                     /locus_tag="ACFMWZ_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC5934500.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            16420..17013
                     /locus_tag="ACFMWZ_000500"
     CDS             16420..17013
                     /locus_tag="ACFMWZ_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC5934501.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            17077..17685
                     /locus_tag="ACFMWZ_000501"
     CDS             17077..17685
                     /locus_tag="ACFMWZ_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC5934502.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            17693..18343
                     /locus_tag="ACFMWZ_000502"
     CDS             17693..18343
                     /locus_tag="ACFMWZ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934503.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            18383..19162
                     /gene="map"
                     /locus_tag="ACFMWZ_000503"
     CDS             18383..19162
                     /gene="map"
                     /locus_tag="ACFMWZ_000503"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC5934504.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            19305..19604
                     /locus_tag="ACFMWZ_000504"
     CDS             19305..19604
                     /locus_tag="ACFMWZ_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC5934505.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            19842..20216
                     /locus_tag="ACFMWZ_000505"
     CDS             19842..20216
                     /locus_tag="ACFMWZ_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC5934506.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(20404..21870)
                     /locus_tag="ACFMWZ_000506"
     CDS             complement(20404..21870)
                     /locus_tag="ACFMWZ_000506"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC5934507.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AGQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         5346 a         6385 c         5548 g         4950 t
ORIGIN      
        1 agaaacatcg agaaaccatg aacatcatct taaacggcgg acccgccgaa cttcacggca
       61 caagcgttgc cgacctcatc gcccaaaccg cgccgcaaca gccctttgcc gtggcggtca
      121 acaccgtttt catccccaaa ggcgcgtatg cggaaacggt tttgaacgaa aacgacaaaa
      181 tcgatatcgt ccgccccgtc gtcggcggct aggcggtttt gccttttcag acgacccctg
      241 tccccaaaac aacgttatgg tggattaact ttaaatcagg acaaggcgac gaagccgcag
      301 acagtacgga tagtacggaa ccgattcact tagtgcttca gcaccttaga gaatcgtttt
      361 ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat acaaaggaac
      421 ccattatgct caccctatac ggagaaactt tcccttcacg gctgctgctc ggcacggctg
      481 cctacccgac ccccgaaatc ctcaaacaat ccatccaaac cgcccagcct gcgatgatta
      541 ccgtctcgct gcgccgcgcg ggaagcggcg gcgaggcgca cggtcagggg ttttggtcgc
      601 tgcttcaaga aaccggcgtt cccgtcctgc cgaacacggc aggctgccaa agcgtgcagg
      661 aagcggtaac gacggcgcaa atggcgcgcg aagtgtttga aaccgattgg ataaaattgg
      721 aactcatcgg agatgacgac accttgcagc cggatgtgtt ccagcttgtc gaagcggcgg
      781 aaatcctgat taaagacggc ttcaaagtgc tgccttattg caccgaagac ctgattgcct
      841 gccgccgcct gctcgacgcg ggctgtcagg cgttgatgcc gtgggcggcc ccgatcggca
      901 caggtttggg cgcggttcac gcctacgcgt tgaacgtcct gcgcgaacgc ctgcccgaca
      961 cgccgctgat tatcgacgcg ggcttgggtt tgccctcaca ggcggcacaa gtgatggaat
     1021 ggggctttga cggcgtgctt ttgaatactg ccgtttcccg cagcggcgat ccggtcaata
     1081 tggcacgcgc cttcgcactc gccgtcgaat ccggacggct ggcatttgaa gccggaccgg
     1141 tcgaagcacg cgacaaagcg caagccagca cgccgacagt cggacagccg ttttggcatt
     1201 cggcggaata ttgaaaaagg cggcaaaaat gccgtctgaa cggttcagac ggcatcgcgg
     1261 cccaaaacgg cggcggtctg aaacgggcaa accgccattc ccaagcatca cggctttgtc
     1321 ggtaaaaatg gaaaaaccgg ccggaaaacc ttgccgcccg tcccgatgcc gcaaccaacg
     1381 aaacactcgg cctccacggt gtgcaggctg ccgcgcaagc cctaaatacg gcaatagaag
     1441 aagcgttttg ttgttgtttg aatacactta aaaataccta aagccgtccg taagccttca
     1501 tccgcaacgg ttttaccgct ttcgcatccc cgaatccacg ctcaagcacc cccgaatgac
     1561 aaccctgtcc gcgccaaatc ggacggatgt tcaaacacgg caggcttatt tccgtcaggc
     1621 acgaagccct cagctatgac tgccgacccc gatttgtccg acacaatgaa agtttgccga
     1681 cccgaatcac aaacatcggc ggacaggtta atttgtttat ttttcatcgt attacaaaaa
     1741 atccgcattt atttttaaat ttttattgat aattattatt attagcgtat aatcaaaacc
     1801 actcggaagt cgtccgttcc gaaccattaa acaccatatt tccccatcat cactttcaca
     1861 cttggagtcg gcatatacga gacatacatt ccctttttat atatcagata ctcaaaaccg
     1921 aaacgccaaa cccaccttcg cggtgggttt ggcgtttatc gtccggcttt cgcgcctatt
     1981 tgcaagactt gaggttcagt ttgccgtata gggacgtgat tttacgaatt tcttccgcat
     2041 cggcggcatt cacgccggta aacaaaaccg tcatacgcga cacgctcaaa gaatcgtcct
     2101 gcctgtcggc ttcggcaaag tgttcgacaa tatgcgcccc gccgaatccg gcgccggcaa
     2161 gccggttttg cagggcttgg gcgttttccc ggctgttgcc gacacccaaa ctcaatgcgc
     2221 cgtcaaacgg tatggggttg aaacctttgg cagacagctc cgccgcctga ttttcggcat
     2281 cggcggaaac gggcaggacg acgcggtagg ttttgtcggc aggtttggct ttggcggtgc
     2341 gtttttcgac gctcctgctg gcaacgtgcg accatttgcc caaaagtcct ttgatgcggt
     2401 ggtagtcatc ttcgtccatc gtgaggcttg cctgcgcggc gcataaggca tccgccgcgc
     2461 cgttttcgcg ttccgcctgc gccttttctg cggcgagttt ttcgcggcgg gctttttctt
     2521 cacgctgttt tttctccctc agttttttct gttccgcttc ctttttcaag cgcaactgct
     2581 cggcctgctc ttcggtcagg atgtcgcccg gtttgagcag tgccgccgta tcctccacgg
     2641 atgccgccgc attgtcggcg gcgggcgttg cggggggcgt gttttcgacg gcccggtttt
     2701 ccggcacgcc gcccgtcgct ttaacggcca atttatagcc caccgtgccg ccgaatacgg
     2761 caatattgag taccgccaaa agggtgaaga gccatttcat tagaatttcc tcaatgtttg
     2821 aaaccccgcc cgcctgggcg gcagtctgtc gggcggcaga atccggacgg attcggacgg
     2881 ataccgcccc gtgttgaaaa agggtgtatt ttttaagtat gttccgattc cccgccttcg
     2941 gcggcaatca ggttcagcag cccgtgaatg acgaggttgt ccgccacgcg cacggtattt
     3001 tccgccaaaa atgcaggcgg cagggcttcg gcaacttttg ccgcgccgcc gccggtaatg
     3061 atgacatcga caggcttgcc cgccccggtt ttttctttca aacgcccgtg catcatcata
     3121 accgagccgc aaaccgcatc catcatgccg ctggcgacgg cattgcccgt tgtggtcggg
     3181 aaaggataac gcttaccggc gtgccggttg aggttggcgg ttcggacggc gagcgattct
     3241 ttcatcaggt ggaaaccggg catgatggtt cccccgagat aatgtccgtc atcggtgagc
     3301 gcgtcaaccg ttaccgccgt gccgcaactg acgacgacgc aggcgttgcg gctgaagcgg
     3361 cggctgccca aggcgttgaa ccagcggtcg gaaccgtgtt cttcggggtg gcggtagtgg
     3421 ttgcgtatgc ccaaagcctg tgcggaagac ggcagccact cgatttttcg ggcgagctgt
     3481 tcctgcactt gtgccttttt gaattctccg cacacggcgc aaccgacgat gcggacattt
     3541 ccatccacct tttccgccca ctccgcgccc aaaggcgaca aatcgcggta cggcgcgcta
     3601 ccgacggttg cgaacgtgcc gttttccacc cacgcccact tgagccggct gttgccgccg
     3661 tccaacagca gaaaacgttc cgaatcccgc cgcttcggca cggaaaccgg cctgtcgtcg
     3721 gaccgcaggc tgatttcgcc gctgacgacc gtctgtttgc cctctgccgt ttccaagtgc
     3781 agaacgcctt gtccgtccac gcctttaacc gtgccttcga acacggtttc gccgtcgcgc
     3841 aacagcaata ccgccttgcc gtggtcgcgg ttggcagcct gatattccgc cacaaaaggc
     3901 gcaaatccgt cccgcgcata ttgcaacaac accgcatcaa gttccgccaa cagcgtttcc
     3961 agcaacacgg cggcatcggc atttccccgc cgcgatgccg tctgaaacag cgattgcacg
     4021 gaagcggcgt tttccacttc cttgggcagc acgaaattga tgccgatacc gaccacggca
     4081 accgttttgc cgcccgtcct gaccgtttca atcagaatgc cgcccaattt gtcgcgtccg
     4141 acgaccaaat cgtttggcca cttgatttgc gttttcaaac ccaaacgcga caaggcgcgc
     4201 cggcacgcca ctgccgcaac aggcgacagc gaacccaact catactgcgg ccggtcaaac
     4261 acccagccaa aactgaacat cagacactcg cccaaacggt gcgaccactt ccgcccctgc
     4321 cgccccctgc ccttactttg caggtgggtc acacatatgg ttttgtgcgc cttgtccggc
     4381 gcaatccgcg ccaattccag tatctcgtcg ttgctggacg cgcactcgtg cttcaatgcc
     4441 gtctgaaaac ccgacctttc ccccagctcg cgcaaacctt cggcatcgaa aaccgccaat
     4501 gggcgcacca gccgccaata gccgtcgtgt tggcgcaaca gcccgcgtat gtgcgccggc
     4561 atctgctgcc aaaaaccgtt gagctgctgc ggcttcatat ccgccatacg cgccagttgc
     4621 gagacgtgtt gcggcaaacc gtcggcaagc tccgccaaca cccgccagtg cgaaagcttc
     4681 aaaaccgtca ttttccgccc tctgccgcac ggatttttgc caaagtcttc gttgtcgaag
     4741 tctggtgcag aaacggaatt gaaaacacct gaccgccgcg cgccaacgtt tctgccgcac
     4801 cgacaatctt atccgcagcc caatcgccgc ccttgaccaa aatctcaggt ttgaccgcct
     4861 caatcaacgc cgccggcgta tccccgtcaa accacgttac caaatccaca ctttccaaag
     4921 cggcggcaac ggcggcacgg ttctccaaag gattaatcgg gcggtcgccg tccttgccca
     4981 aacgccgcac cgaagcatcg gtattcaacg ccagcaccaa cgccgcccct gccgaacgcg
     5041 cctgcgccag ataagtaacg tgccccctgt ggaggatgtc gaaacagccg ttggtaaaca
     5101 ccagcgggcg cggcaaccga gccaaccgtt cggacaatgc ctcgggcgga cagattttcg
     5161 attcaaaatc agggacagac caagcgtcaa ccatcaaagc ctccgacaaa aaccataaaa
     5221 gacagaaaaa cccacatgat acagaagcat atgcgaaagg caaagccggc ggcgcggaca
     5281 gtacgcgcaa acgggaaaag acccgtaccg aaaagtacgg gcctttatct ggggtggctg
     5341 atggggctcg aacccacgac aaccggaatc acaatccggg gctctaccaa ctgagctaca
     5401 gccaccataa aaacggtttt caatcaaatt cttggcacgc ccgacaggaa tcgaacctgt
     5461 aacccccgac ttagaaggtc ggtgctctat ccggttgagc tacgggcgct catgccgatt
     5521 cgtgctgatt gattggtcgg ggcggtggga ttcgaactca cgaccctctg ctcccaaagc
     5581 agatgcgcta accgggctgc gctacgcccc gacttgaaga agcgaactat acaactcagg
     5641 gaaagatgcg tcaacattta ttttcaagac accaagatga aaaatatagt tttttgattt
     5701 gaaaaaatat ttaatccgtc caaacagccg tattttattt cagggcaaat ttattttcgg
     5761 catcctgctg taaaaacaaa cggaaaatgc gataattttc agcattttct acctgtttaa
     5821 caaaaggacg gatatgtcgg cacaactgat caatggtaaa gaagtttcgc aaaaacgcct
     5881 gcaggcggtt gccgaagcgg tggcgcaacg ccaacaggac aatctgcaca tgccttgcct
     5941 ggccgtggtt ttggtcggag gcgaccctgc cagcgcggtt tatgtccgca acaagaaaac
     6001 tgcctgccaa aaatgcggca tcaaatcact gtcttacgag ctgcccgaat caacatcgca
     6061 ggaagaacta ctggcactgg tcgaccgcct gaatgccgat tccgaagtgg acggtattct
     6121 ggttcagcta ccgctgccga agcacctcga cagccaggcg gttttggaac gtatttcgcc
     6181 ggataaggac gtggacggct tccatcctta caatgtcggc aggctggcgg tcaaaatgcc
     6241 gctgatgcgc ccgtgtacgc ccaagggcgt gatgacgctt ttggaagctt acggcattga
     6301 tccgaagggg aaaaaagcgg tcgtggtcgg cgcgtcgaat atcgtcggcc gcccgcaggc
     6361 tttggaactg ctgctggcgc gcgcaacggt aacggtctgc cacagcgcaa ccgaaaatct
     6421 gacagacgag gttgccggag ccgatatttt ggtggtcggc gtaggtattc cgaactttgt
     6481 caaaggcgaa tggatcaaac ctggcgcggt cgttattgat gtgggcatca accgtttgga
     6541 cgatggcagc ctgtgcggcg acgtggaatt tgaaacggca aaagaacggg cggcgatgat
     6601 tacgcccgtt cccggcggcg tgggtccgat gacgattgcc acattgatgg aaaacaccct
     6661 gcacgcggct tcactgcacg atgcttgagc ggttctgaag ataaaaatgc cgtctgaaag
     6721 gctttcagac ggcattttgc cgtgtccgtt tatttgggca gcttgacgac aaccgtatcc
     6781 gccagtatgt cgtaaagcgt gcggcgatcg cgtttgacca taaagagcag gacaaagttg
     6841 gcaaggaatg ccagcaggtt gatggcgttt tctccgttgt cacctactgc aagaccgata
     6901 acggcggcaa taatggcaac caaaaccgac catgcgattt cgcgtaccaa aaccgtgccg
     6961 acaaaaccgg gattgcggcc gtcggttttc aacaccctga ttctcatgat tttcttaccc
     7021 aatgactgcc cgtcccgact catatagtag atttgaacga cggtataaac caaaatgccc
     7081 accagtccta cccaaaagga agtcatgccc aaaagcagcc cgaatatttc ctcactgccg
     7141 ccaatcctgc cttcattctt gatggcgaaa gcaatcagtc cggcaaacgg caccaacaaa
     7201 accaaaaagg taaacaattg gttcagcagc gcggcaagta tccggtcgcc tgcgccggca
     7261 attccgactt caatttcctg cccgttgcgg ttgtcggatg ccgcgtcggt gtagtcgttt
     7321 ttttcttcca tatccgttcc tgataattgt tcttaactga ccccgattct accgccacga
     7381 caccgaaaac gccaatactt aaagaaatcc cgataaagaa ctttacattt tcccaatacg
     7441 gcgttaaaac gcttccttta cgccatacat aattttatta acgatttttc ctcaaggagc
     7501 aacacaatga aagtaggttt cgtcggctgg cgcggtatgg tcggttcggt tttgatgcag
     7561 cgtatgaaag aagaaaacga cttcgcccac attcccgaag cgtttttctt taccacttcc
     7621 aacgtcggcg gcgcagcccc tgatttcggt caggcggcta aaacattatt ggacgcgaac
     7681 aacgttgccg agctggcaaa aatggacatc atcgttacct gccaaggcgg cgactacacc
     7741 aaatccgtct tccaagccct gcgcgacagc ggctggaacg gctactggat tgacgcggca
     7801 tcctcgctgc gtatgaaaga cgacgcgatt atcgtcctcg accccgtcaa ccgcaacgtc
     7861 atcgacaacg gcctcaaaaa cggcgtgaaa aactacatcg gcggcaactg taccgtttcc
     7921 ctgatgctga tggctttggg cggcctgttc caaaacgatt tggtcgaatg ggcaaccagc
     7981 atgacctacc aagccgcttc cggcgcaggc gcgaaaaaca tgcgcgaact catcagcggt
     8041 atgggcgcgg ttcacgccca agtggcggac gaacttgccg atcctgccgg ctcgattctc
     8101 gacatcgacc gcaaagtatc tgatttcctg cgcagcgaag actatccgaa agccaacttc
     8161 ggcgtaccgc tcgccggcag cctgattccg tggattgacg tggatttggg caacggccag
     8221 tccaaagaag aatggaaagg cggcgcggaa accaacaaaa tcctcggccg cagcgacaat
     8281 ccaaccgtga ttgacggcct gtgcgtccgc atcggctcta tgcgctgcca cagccaagcc
     8341 cttaccctga agttgaaaaa agacctgccc atttccgaaa tcgaagcgat tttggcaggc
     8401 gcgaacgact gggtgaaagt catccccaac gaaaaagaag ccagcatccg cgagctgacc
     8461 cccgccaaag ttaccggcac gctgtccgtc cccgtcggac gcatccgcaa actgggtatg
     8521 ggcggcgaat acatcagcgc gttcaccgtc ggcgaccagc tcttgtgggg cgcggccgag
     8581 ccgctgcgcc gcgtattgcg tatcgtgttg ggcagcctgt aagccctgtt tgaatggaaa
     8641 tgccgtctga agcctgtttc agacggcatt ttccttgcaa ccccagttaa ccgaaattcc
     8701 gataaggcaa gcgttcacgc cagcaacatt tcctgcatca gcttcatacc ccactgccag
     8761 ccgccgagca tgccgttcaa actgcccgaa tgcggggaaa ccaacaggcg ggcgttccac
     8821 aaatccgcct gtttttgcgc ccaaccgtgc ggcacgccgc cgtgttcggg tacaaccaat
     8881 gcggcacggc agggacagcg gacgcgttgg aaagtgtgtt ccgcatcgtc gggaaaaata
     8941 tcgggacgct gcggtacaag gatgatgttg gcaattttct tccgtgtcag gatgtctgcc
     9001 tgatacagcc acgccaaaaa tgcggccgcg cccgcaccgt gtgcgacaac ggcgacgtat
     9061 ttgccacgta tgcgttcaaa tgccgtccga agccctgcct gccattcccc tatgctttga
     9121 ccggccgacg cttcggacat ctgcacgacg ggataactga tcgcccaacg gtctatccac
     9181 atctgatcct ctccggcatc gcgtatcagc caaagcgtca aatcttcgag ttcaaaaccc
     9241 tgcataccgc cccgcctatt tcagcaggtc ccggagggta aaggcgatga gcagcgaagc
     9301 gggtacgctc aatatggcgc agacggtcag gcaggcaaaa atattcacca cccgccgcca
     9361 gcctttccaa cccaaacatt tggacgcaat cagcaggcag ggcatgcaga tcagcagcca
     9421 caccaacgcc catgtcgggt ttgccttggt cggcgcaagc cagccttgca tccgcgacaa
     9481 cataaatatc gcccacacca atatgggcag gataaacgca gcgacaaccc atgctgcgcc
     9541 tattcctgtt tttccgtcca cattccaatc atatttaccc aaaactttat tcggcagcat
     9601 agtcatactc catgaccagc ggcgcatggt cggaaaattt ttcatcttta taaacgtgtg
     9661 cggacacggc tttggcggca agttcgggcg taaccatctg ataatcgatg cgccacccga
     9721 catctttcgc atacgcctgc ccgcggttgc tccaccacgt gtagcccggc acatcgggat
     9781 aaagcgtgcg ccacatatcc gtccaaccga gcttgtggat aaccttgcct atccactcgc
     9841 gttcttccgg taggaagccc gaatttttct gattgccttt ccagtttttc aggtcgatgt
     9901 tttggtgggc gatgttccag tcgccgcaaa cgacaatgtc gcgtccttcg tttttcatcg
     9961 cttcgagcat agggtaaaac gcatcaagga aacggtattt cacctgctgg cgttcttccg
    10021 cgctgctgcc gctgggcaag taaagcgaaa taacgctcaa cctgtcgaaa tcgcaacgca
    10081 caaaccgccc ttccctgtcg aattcttcaa tgcccatacc gatttgcaca ttgtcgggtt
    10141 tgcgtttgct gtacaccgcc acgccgctgt aaccgcgctt ctcggcgcaa tgccaatagc
    10201 cgtgcatccc atgcggattt ttcatatcgg cagacaaatc agcctcctgc gctttgagtt
    10261 cctgcacgca gacaatgtcc gcgcccgatg cggcgatgta ttcgtaaaaa ccttttttgt
    10321 aggcggagcg gatgccgttg acgttggcgg aaatgatttt aagcataata ggaaatgtgt
    10381 tctcgcaata aaaaatgccg tctgcaaaca tagaaaaggc aagagacagc actcctgccc
    10441 tttaccgtac cccttaaccg cgccgccaag tcgtgccgcc ggcgttgtct tccaaaatga
    10501 ttttgtgttc gttcagaagg tcgcgaatgc ggtcggattc cgcccagttt ttatcggagc
    10561 gcgcctgttt ccgccgggca atcaagtctt cgatttcttc gttggagagg ccgtctgaag
    10621 ccgcgccgcc ttgcaggaac tcggtcgggt cgcgttgcag cagtccgatg atgccgccca
    10681 aggctttcag gcagccggcc agttgcgcgt cattggtttt gttcacttcg ccggcaagtt
    10741 cgaacaacac cgccaccgct ttcaccgtat caaaatcatc attcatcgca acataaaagc
    10801 ggcgcgtgta gtcatcgccg gcttcagacg gcatcggatc ggcgggcggc gtatttttca
    10861 aagtcgtata caaacgcgtc aacgcgcctt ttgcatcatc caaatgcgcg tcggaatagt
    10921 tcaacgggct gcggtagtgg gcgcgcagga tgaagaagcg cacgacttcc ggatcgtatt
    10981 gtttcaacac ttcgcggatg gtgaagaagt tgcccagcga tttggacatc ttttcgccgt
    11041 ccacgcggat aaagccgttg tgcagccagt atttgacgtg gctggcgatg ctttgcccgt
    11101 ggtgggtttg cgcgtgatga tgaccgcagg tatgccccgt cgcgccgacg ctttgggcaa
    11161 tttcgttttc gtggtgcgga aactgcaaat ccgcgccgcc gccgtggatg tcgaaggtat
    11221 cgccgaacag gttttcactc atggcagagc attcaatgtg ccaacccgga cggccgttgc
    11281 cccacgggct ttcccacgcc ggttcgcctg ctttcgccgc tttccacaat acaaaatcaa
    11341 gcggatcgcg tttgaaaccg tccacttcca cgcgttcgcc cgcgcgcagg tcgtccaacg
    11401 atttgcccga cagttgtccg taagcggcaa actcgcgcac ggcgtagtaa acgtcgccat
    11461 ttgcggcagg atatgccttg ccgttttgaa tcagggtttc aatcatggca atcatttgcg
    11521 ggatgttttc cgtcgccttc ggctcgatat cgggacgcaa cacacccaaa gcatcggcat
    11581 cttcgtgcat agcctgaatg aaacgcgcag tcagctcgcc gatggtctcg ccgttttcag
    11641 ccgcacgggc aatgatttta tcgtcgatgt cggtgatgtt gcgtacataa gtgagcggat
    11701 agccgcactc acgcaaccaa cgggcaatca tgtcgaacac aaccatcaca cgggcatggc
    11761 ctaagtggca atagtcgtaa acggtcatgc cgcaaacgta catacgcacg ttttcagggt
    11821 cgatgggggc aaagggttct ttttggcggg taagggtgtt gtagatggtg gtcatgggat
    11881 tatggattaa tctttgttgc tcggatgata atttctgttc tgttcctgta gatacggacc
    11941 aaggaacatt acgcagttgc ggattattaa tatggctgat atttgtgaaa attggttctg
    12001 cataacagtt tgcaaaattt tttgtaaatt ctgataattt aaacttatct tttaataagt
    12061 ttgctaaatc tgatgacgag ggataaagtt tacttcttat actaggcatt tcaatatgaa
    12121 ggactatttt tatttcgtta caatctaaag ccaagcgaga aaaatctttt tcttcctgtt
    12181 ttgctgcttt aaatttagca gaaaccaatc ctgccaatga atctcgaatt tttcttgcga
    12241 ttaaatatgg taggtcagaa agtttttcat ctattgattg ggcatttggc tcaagcccaa
    12301 gtcggtaata atctttaatt tcgattagcc aaagtgtcga ctcatgaagg gctattatat
    12361 ctacacctga gctgccatta tcgtcatcta cactttgatt tatcccgttc tttccctttt
    12421 catttgtatc aattttatta cgtaaattac aactgttctg aaaaatttta taatgttccc
    12481 attcgtcata cttggtaacg taataatctt caggaaaagc aaaggttaat ctcttttctg
    12541 tgattgtagt catagcttaa cctcaaatat tcagatacct gtctgcctgc ataatgtttt
    12601 catctaacaa tatcaatgtg ttcaaatcat taatactgtt cccttgctcc acttttgttc
    12661 catcatcgga agcaatcaac gagaaaaaac gtacaggtaa atccgtgtta ttttcaagct
    12721 tcaaaagttc caattctctc aataagaata aagagtgtgt tgcaataaaa acctgaatac
    12781 cctgttgaga taaagaccaa ataatacggg cagccacttt gatcaattta ggattcagat
    12841 tagcttccgg ttcatcccaa aatagatagc ctttatccag caatgcccct gttgcgatta
    12901 accgggcaat catgacaaat ttccgcaaac cctctgctac caaaggtgct tcaatcttac
    12961 cgcccgtatt tgtcagcgat agataaaacc ttccttgttc ttcagatact tttccgccca
    13021 tcgcgttctc aataggttcg agcaattctc gaatttttgt ttctctgggg cctttggcaa
    13081 gcgggtgatt taattgcata caggcatcaa accaagtttc ttcgaaaggg atgctttggt
    13141 tttgatacaa agaagtgaac caagggcaaa gtgtaattaa ttcgcggctg ggtaagaaga
    13201 taggtgtcgg agtatattca ttttctttca atccgatgct ttgaacattg acttgcgatg
    13261 atgagttact ggaaaaattc agactactat gcgtagtgcc gttttgcagt tttaaaacga
    13321 tttccgtacg cccgcgcccc tgcaaacgtt tgctcaacct gcccaaggaa tcgggacgga
    13381 aaacattcag taatttatcg gcaaaacttt tttgcaattc tgttttcagt aatctgtttt
    13441 tggtgttaga tgttacttct agcaggctgt ataaaatttt taacaaatgt gttttgccac
    13501 aaccgttttc ggcaacaata acattgagat tttcagaaaa ttcaaaagta tcgtttggaa
    13561 gaacggtaaa gtttgtcaac tcaagcgact ggatatattg gttagatgac atttttaatc
    13621 catttcaatc ttgctttaaa attgtttcaa acaacctttt gtagaacaaa tatcgtctga
    13681 aaccctttct tttttcactc cggcttaaac acgcccgtat ccgttttagg ctgctgttcg
    13741 ataatttcaa catttgccgc tgctttctcc gcttcggctt tttcagcttc gatacgtttt
    13801 ttctcggtca ggtattggtt gatttggtgt accaattcct gcgtgccttg gtgggtcagc
    13861 gcgctgattt ggaagaggcg cggggtttcc atgtcaaatt ggaaacggtc gtcgggtttg
    13921 gggtagtccc agccgacggc ttcgaggaag gcggctgttc gcgcccgggc ttcttcttca
    13981 tcgagcatgt cgagtttgtt cagcaccagc cagcgtggtt tgccgtagag ttcttcgtcg
    14041 tatttgcgca attcgttgat gatggcgagg gcttcttcgg cggggttgac ggtttcgtca
    14101 aacggggcca aatcgacgac gtgcagcagc aggccggtac gtgataagtg tttgaggaaa
    14161 cgatggccga ggcctgcgcc ttctgccgcg ccttcaatca ggccggggat gtcggccatc
    14221 acgaagctgt ggttttcgtc gatgcgtacc acgcctaagt ttggatgcag ggtggtaaag
    14281 gggtagttgg cgattttggg gcatgcggcg gagacggcgg taatcagggt ggatttgccg
    14341 gcgttgggca tgcctaacag gccgacatcg gcgaggactt tgagttcgag ttgcagggaa
    14401 cgggtttcgc cttcttcgcc gggcgtggat tgttttgggg cgcggttgac ggaggatttg
    14461 aagtggatgt tgcccaagcc gcctttgccg cctttggcta ggcagacgcg ctgtccgtgg
    14521 taagtgaggt cggcaacgat ttcgtcggtg tcgaggtcgc ggatgagggt accgacgggc
    14581 attttgagga cgatgtcgtc cgcacctgcg ccgtaacggt cggaaccgtg gcctttttcg
    14641 ccgtttttgg cttggtagcg tttaacgaag cggtattcga cgagggtgtt ggtgttttcg
    14701 tcggcttccg cccagacgct accgcctttg ccgccgtcgc cgccgtccgg gccgccgcgc
    14761 ggtacgaatt tttcgcggcg gaaactggtt gcgccattac cgcctttgcc tgcggcgact
    14821 tcgatttttg cttcgtcgat gaatttcatt caatgctctt gtttgttggt ttcaaatggg
    14881 ggttcagacg gattaccgtg tgttttgatg ccgtccgaac agaatttccg ccggtattat
    14941 aaggtataag cggtttttca cataccgtgg ggcggcgatt cgccctgcct gccccgatgg
    15001 ttttgaaacg aatctacgcc ccggaagcaa aatatgcccg ccgccttcgg tacaggcgcg
    15061 ccttgccgat atggcgttaa ccgggtcttt ccggacggaa aaccatccat ccgcacaccg
    15121 cccgaagcgg caaaagacta aatcaaatct ttgtttttac aggataaagc aaaatcgtac
    15181 aaagcttttt ttccctcgcc cgtgattttg gcggcaagct ccgccgcctg tttggtcggc
    15241 agctcggcag caaggatttt catcacattt tgcgcggact cggacaagcc ttcgtgtttt
    15301 tcatcctgcg ctggataaag caccaacacc atctcaccgc gcgattggtt gccgtccgcc
    15361 gccaatgctg tctgaatttc cccaaccgtg ccgcttaaga acgtttcaaa cgttttcgta
    15421 atttcgcgcg ccagcatcag acggcgttcg gggagcagtt ccaccatatc ggcaagcgtc
    15481 gccccgatgc ggtgcggcgt ttcaaacatg acgataggaa acgccgcccg cacccatttg
    15541 gcaaacaatt tcctacgttc gcccgatttc ggcggtacaa aaccgttgaa ataaaaatcg
    15601 gatcccgcca caccagccac actcaaagcc gccatcaccg cgcttgcgcc gacaacaggg
    15661 acaactttaa acccgacctc acgcacgcgg cgggcgagtt tcgcgcccgg gtcgcacacc
    15721 gccggcgtac ccgcatcgga aacctgtgcc acaaccatgc cgtctgaaag acagccgaca
    15781 atcttgtccg ccatctgccg ttcgttgtgt tcgcgcacac tgacgagttt gccctgaatg
    15841 ccgtacgcgc tcaaaagctg cgcggtaacg cgcgtgtctt cggcacagat gatgtccgcc
    15901 ttttgcaata ccgccaaagc acgcagggta atgtccgcca aattgccgat gggcgtggca
    15961 accacgtata atgtccctcc gacgacgctg tcggaggctt tctgcaaatg tttctgaaac
    16021 ataagaatgc cgtctgaaaa acaaacatta taaaggttaa accgattatg cgcctaaacc
    16081 acaaacaggg cgaggcaggg gaagatgccg cgcttgcctt cctccaatcc caaggctgca
    16141 cactgcttgc ccgcaactgg cactgcgcct acggcgaaat cgacctgatt gtcaaaaacg
    16201 gcggcatgat tctgtttgtt gaagtaaaat accgcaaaaa tcggcaattc ggcggtgccg
    16261 catacagcat ttccccatcc aaattattga aactgcaacg aagtgtagag tattatctgc
    16321 aacagaacag gttaacaaac gtaccgtgcc gcctcgacgc ggtactcatc gaaggcagcc
    16381 gcccgcccga gtggatacag aatattacag gttgacgata tgacgacatt acaagaacgc
    16441 gttgccgccc attttgccga aagcatccgt gccaaacagg aagccgaaaa agtactggtc
    16501 gagccgaccg tacaggctgc cgagctgatg ctgcaatgcc tgatgaatga cggcaaaatc
    16561 ctggcctgcg gaaacggcgg ttcggctgcc gacgcgcaac acttcgccgc cgaaatgacc
    16621 ggtcgttttg aaaaagaacg catggaactc gccgccgtcg cgctgacaac agacacttcc
    16681 gcgctgaccg ccatcggcaa tgactacggc ttcgaccacg tattcagcaa acaggtgcgc
    16741 gcgctcggac gtgcaggcga tgtattggtc ggcatttcca cctccggcaa ttccgccaac
    16801 gtcatcgaag ccgtcaaagc cgcacacgaa cgcgacatgc acgtcatcgc cctgaccggc
    16861 cgcgacggcg gcaaaatcgc cgccatactc aaagacaccg acgttttgct caacgttccc
    16921 catccgcgca ccgcccgtat tcaagaaaac cacatcctgc tgatacacgc catgtgcgac
    16981 tgtatcgact ccgtactgct ggaaggaatg taaccctttt cagacggcat ggcgcaaagc
    17041 aatgccgtct gaaacgccca agaaaggaag cacccgatga aacccaaacc gcacaccgtc
    17101 cgcaccctga ttgccgccat tttcagcctt gcccttagcg gctgcgtcag cgcagtaatc
    17161 ggaagcgccg ccgtcggcgc gaaatccgcc gtcgaccgcc gaaccaccgg cgcgcaaacc
    17221 gacgacaacg ttatggcgtt gcgtatcgaa accaccgccc gctcctatct gcgccaaaac
    17281 aaccaaacca aaggctacac gccccaaatc tccgttgtcg gctacaaccg ccacctgctg
    17341 ctgctcggac aagtcgccac cgaaggcgag aaacagttcg tcggtcagat tgcacgttcc
    17401 gaacaggccg ccgaaggcgt gtacaactac attaccgtcg cctccctgcc gcgcactgcc
    17461 ggcgacatcg ccggcgacac ttggaacaca tccaaagtcc gcgccacgct gttgggcatc
    17521 agccccgcca cacaggcgcg cgtcaaaatc gttacctacg gcaacgtaac ctacgttatg
    17581 ggcatcctca cccccgaaga acaggcgcag attacccaaa aagtcagcac caccgtcggc
    17641 gtacaaaaag tcatcaccct ctaccaaaac tacgtccaac gctgactcgg caatgccgtc
    17701 tgaaccgcct tcagacggca ttgcccgaca ccccaaaagc acaatcaaaa tggcaaaaaa
    17761 accgaacaaa cccttcaggc tgacccccaa actcctgata cgcgccgtat tactcatctg
    17821 tatcaccgcc atcggcgcat tggcaatagg catcgtcagc acattcaacc cgaacggcgg
    17881 caaaaccctc caaaccgaac cgcaacacac cgacagcccc cgcgaaaccg aattctggct
    17941 gccaaacggc gtagtcggac aagatgccgc ccaacccgaa caccaccacg ccgcctcatc
    18001 cgaacccgca cagccggacg gcacagacga aagcggcagc ggactgccgt cccctgccgc
    18061 acccaagaaa aaccgggtca aaccgcaacc tgccgacaca gctcaaaccg acaggcagcc
    18121 ggacgacgcc ggaacacaag ctgaaaacac actcaaagaa acccccgtac tgcccacaaa
    18181 cgtcccccgt cccgaacccc gaaaagaaac acccgaaaaa caggcgcagc ccaaagaaac
    18241 acccaaagaa aaagaaacgc ccaaagaaaa ccataccaaa ccggacaccc cgaaaaacac
    18301 gccgcccaaa ccccataaag aaattctcga caacctcttc tgacccggca cggcaggcac
    18361 acccgcaatc caaggaagca ttatgaacgg catcatcatc aaaacccccg aagaaatcga
    18421 aaaaatgcgc gagctgggca aactcgtcgc cgaagccctc gactacatcg gacaattcgt
    18481 caaacccggc gtaaccaccg acgaaatcga caaactcgtt tacgactacc acgtcaacgt
    18541 ccaaggcggc tatcccgccc ccctgcacta cggcaacccg ccctacccca aatcctgctg
    18601 cacctccgtc aaccacgtca tctgccacgg cattcccgac gacaagccgc tcaaagaagg
    18661 cgacattatc aacatcgacc tcaccatcaa aaaagacggc ttccacggcg actccagccg
    18721 tatgtttacc gtcggcaaag tctcccccat cgcccaacgc ctgatcgacg taacccacgc
    18781 ctccatgatg gcgggcatag aagccgtcaa acccggcgcg acactgggcg acgtaggtta
    18841 cgcctgccaa caggttgccg aaaacgccgg ctattccgtc gtacaggaat tctgcggaca
    18901 cggcatcggg cgcggtttcc acgaagcccc gcaagtgttg cactacggaa aaaaagggca
    18961 gggccccgtt ctaaaaccgg gtatgatttt taccgtcgaa ccgatgatca accaaggcaa
    19021 acgccacctg cgtatcctca acgacggctg gacggtggtt accaaagacc gctccctctc
    19081 cgcccaatgg gaacacgaag ttttggttac cgaaaccggc tacgaaatcc tcaccgtcag
    19141 cccggccacc ggcaaaccct aaccccccta taaaaacaaa caatgccgtc tgaaagaaac
    19201 agcagatata atatgcaata taaaaacagg cttgacccgg cacattacga aaacaaagca
    19261 aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag ttttatgaaa atatttgaaa
    19321 atatagaaga tgttaaagcc atccgtaaaa agaccgggct gaaccagata gacttctggg
    19381 gcaaggtcgg cgttacccag tctggaggct cgcgctacga aaccggccgc aaaatgccca
    19441 aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg catcgatttg gcgaaagtca
    19501 acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca ccatcccgac ctgtatgccg
    19561 aattgtccaa acagaccaag tccgaaagaa aaaaacaaag ttaaaccgca acctccggat
    19621 gcccgaaggt ttttcatttc cgaaaaacgc aaacaatgcc gtctgaaaca ccggacaggc
    19681 cgccgtatcc cgcctgccgc ccctgcctca aaccgccgaa ccgcccgaac ccgccttttt
    19741 acaaacttta tccaatttcc tgtttatttc aggatacgcc gacattagaa tgtcaaacag
    19801 ctcgaaacgg gcaaactcca catccatcca aaggaataaa aatgaaactt ctgaccaccg
    19861 caatcctgtc ttccgcaatc gcgctcagca gtatggctgc tgctgccggc acgaacaacc
    19921 ccaccgttgc caaaaaaacc gtcagctacg tctgccagca aggtaaaaaa gtcaaagtaa
    19981 cctacggctt caacaaacag ggcctgacca catacgcttc cgccgtcatc aacggcaaac
    20041 gcgtgcaaat gcctgtcaat ttggacaaat ccgacaatgt ggaaacattc tacggcaaag
    20101 aaggcggtta tgttttgggt accggcgtga tggatggcaa atcttaccgc aaacagccca
    20161 ttatgattac cgcacctgac aaccaaatcg tcttcaaaga ctgttcccca cgttaatcag
    20221 gcaacaaaaa acagcgtttt cagaaatgaa aacgctgttt ttttgaccgt tccattattc
    20281 gcaaaaggga aaaaacgatt acctgccccg tgtatcaaaa cctgccctgc cggatgaagg
    20341 gcataaccgg cagggacggc atcaacacca tatgggggta cggcttttct tgaaagattc
    20401 ggcttaaata tccaacactt tcgcggtata ggcgataatt tcatccgccc tttcagggtt
    20461 ttcgttcaac ttgatgccgt aacccggtac cagctctttc agacgacctt cccaagacgg
    20521 ggcgcgctcg gggaagcatt ggtgcatcag ccggatcatc agcggcacag cggtcgatgc
    20581 gcccggcgac gcgcccagca atgcggcgag tgagccgtcg gcgtgggcga caatctccgt
    20641 accaaactgg agcacgccgc ctttttcgga gtctttttta atgatttgga cgcgttgccc
    20701 tgcggtgatg agttcccagt cgtcggggtt tgcctcgggg tagtattcca gcagggaggc
    20761 gaagcgttct tctttggttt tacgcaattc gcccagcagg tatttggtca gcggcatatt
    20821 cgcccagccg gcgcgcagca taggatagag attgtccata tggatggaca gcggcaaatc
    20881 cataagcgag ccttgcttga ggaagttgga acggaagcct gcgtaagggc cgaacataag
    20941 gtggcgtttg ccgtccacgt tgcgtgtgtc gaggtgcggg acggacatcg gcggcgcgcc
    21001 gacggaagcc tgcccgtaca ctttggcgtt gtgttgtccg gcggtttcgg ggttgctgtt
    21061 gcggaagaac aggccggaca cggggaagcc gccgtagcct ttgccttcgg ggatgccgga
    21121 tttttgcagc agggtcagcg cgccgccgcc cgcgccgagg aagaggaagc gggtacggag
    21181 ggtgagctgc ccgtcggggt tgcgggtatc ggcggttttg agcacccacg cgccgtcgga
    21241 ttcgcgtttg atgtcttcga cgtggcggtt gaactcggtt tttacgccct tgccctgcaa
    21301 atatttcacc atttggcgcg tcagccgtcc gaaatcgaca tccgtgcctt cggcggagta
    21361 gttggcggcg acgggttggt tttcgtcccg gccgcgcatc atcagcggag cccaatcgga
    21421 aattttgttc cgatcggtgg aaaattccat attttcaaaa agtttttggg ttttaaacgc
    21481 gtcataacgt ttttgaagat aagaacaatg gtcttcattc atcaccaaag acatatgcgg
    21541 cacggcattg atgaaggaat tgtcttccaa cttgccttcc gcgaccagcg tcgcccaaaa
    21601 ctggcggctg acatgaaact gttcggcaat attgagggcg cgcgccggat cgataatccc
    21661 atttgcaccc aacggcgcat agttcaattc gcacagcgcg gaatgccccg tgccggcgtt
    21721 gttccacgcg tttgacgatt ccaacgccac atcttccaag cgttcaatca gggtgatttc
    21781 ccaagacggt tcgagttctt tgagcaaaac gcccaaagtc gcgctcataa tgccgccgcc
    21841 caccaagaca acgtctgtcg cttcagccat ggtttactcc taaaaaacag gcatcttctg
    21901 cccttatggt tatttgccgt actacaaacg cctgaatcgc aaaagcaggg aaaaccggca
    21961 atggtgtgtg tccgagtatg ctgtttcggg gttggaatgc gttgcaagta tggcttccga
    22021 caccgcttca ggggcttgta atatgttatc gtgaatgtag tggattttac tgggaaatgc
    22081 aaagtttttc tgtcgcccgc caagtcggga aactgcgaaa tgaaaaataa aaatagttat
    22141 ttatctatat atatcaaatt tttaatagat aaaaaatcaa aattgtttat atattaattt
    22201 ttaaaagatt gtcagcatat tgcgttaag
//
LOCUS       DAXHKX010000015        21510 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHKX010000015 DAXHKX010000000
VERSION     DAXHKX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.015"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(60..1946)
                     /gene="uvrC"
                     /locus_tag="ACFMWZ_000507"
     CDS             complement(60..1946)
                     /gene="uvrC"
                     /locus_tag="ACFMWZ_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC5934508.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IINNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSEIPGIGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2001..4178)
                     /locus_tag="ACFMWZ_000508"
     CDS             complement(2001..4178)
                     /locus_tag="ACFMWZ_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC5934509.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4317..5267
                     /gene="trxB"
                     /locus_tag="ACFMWZ_000509"
     CDS             4317..5267
                     /gene="trxB"
                     /locus_tag="ACFMWZ_000509"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC5934510.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5423..5791
                     /gene="rpsF"
                     /locus_tag="ACFMWZ_000510"
     CDS             5423..5791
                     /gene="rpsF"
                     /locus_tag="ACFMWZ_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC5934511.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5792..6094
                     /gene="priB"
                     /locus_tag="ACFMWZ_000511"
     CDS             5792..6094
                     /gene="priB"
                     /locus_tag="ACFMWZ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC5934512.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6101..6331
                     /gene="rpsR"
                     /locus_tag="ACFMWZ_000512"
     CDS             6101..6331
                     /gene="rpsR"
                     /locus_tag="ACFMWZ_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC5934513.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6348..6800
                     /gene="rplI"
                     /locus_tag="ACFMWZ_000513"
     CDS             6348..6800
                     /gene="rplI"
                     /locus_tag="ACFMWZ_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC5934514.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEVRRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            6814..6921
                     /locus_tag="ACFMWZ_000514"
     CDS             6814..6921
                     /locus_tag="ACFMWZ_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFC5934515.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKVGMVVFIVD"
     gene            complement(7588..8394)
                     /locus_tag="ACFMWZ_000515"
     CDS             complement(7588..8394)
                     /locus_tag="ACFMWZ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC5934516.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPKLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8395..9141)
                     /gene="pssA"
                     /locus_tag="ACFMWZ_000516"
     CDS             complement(8395..9141)
                     /gene="pssA"
                     /locus_tag="ACFMWZ_000516"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC5934517.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(9215..9619)
                     /locus_tag="ACFMWZ_000517"
     CDS             complement(9215..9619)
                     /locus_tag="ACFMWZ_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934518.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            9725..10207
                     /locus_tag="ACFMWZ_000518"
     CDS             9725..10207
                     /locus_tag="ACFMWZ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC5934519.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGALR
                     VDNAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(10259..11470)
                     /locus_tag="ACFMWZ_000519"
     CDS             complement(10259..11470)
                     /locus_tag="ACFMWZ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC5934520.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSIGEWGLPSTMFGLFAAGFMYFVFAVLIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11693..14131
                     /locus_tag="ACFMWZ_000520"
     CDS             11693..14131
                     /locus_tag="ACFMWZ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC5934521.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            14340..15650
                     /gene="tig"
                     /locus_tag="ACFMWZ_000521"
     CDS             14340..15650
                     /gene="tig"
                     /locus_tag="ACFMWZ_000521"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC5934522.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKVPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQVPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15746..16360
                     /gene="clpP"
                     /locus_tag="ACFMWZ_000522"
     CDS             15746..16360
                     /gene="clpP"
                     /locus_tag="ACFMWZ_000522"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC5934523.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQL"
     gene            complement(16414..17679)
                     /gene="ispG"
                     /locus_tag="ACFMWZ_000523"
                     /gene_synonym="gcpE"
     CDS             complement(16414..17679)
                     /gene="ispG"
                     /locus_tag="ACFMWZ_000523"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC5934524.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKITLKGDNIASEFL
                     AIVEEYVKTNYGENSSKRNKGKVIPIQSL"
     gene            complement(17695..18456)
                     /gene="pilW"
                     /locus_tag="ACFMWZ_000524"
     CDS             complement(17695..18456)
                     /gene="pilW"
                     /locus_tag="ACFMWZ_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC5934525.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18459..19553)
                     /gene="rlmN"
                     /locus_tag="ACFMWZ_000525"
     CDS             complement(18459..19553)
                     /gene="rlmN"
                     /locus_tag="ACFMWZ_000525"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC5934526.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMIPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19697..20122)
                     /gene="ndk"
                     /locus_tag="ACFMWZ_000526"
     CDS             complement(19697..20122)
                     /gene="ndk"
                     /locus_tag="ACFMWZ_000526"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC5934527.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(20181..21332)
                     /gene="zapE"
                     /locus_tag="ACFMWZ_000527"
     CDS             complement(20181..21332)
                     /gene="zapE"
                     /locus_tag="ACFMWZ_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC5934528.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5157 a         5330 c         5848 g         5175 t
ORIGIN      
        1 tcgatatacc gacctgctgt atcatatttt ttacagacgg attttgcttg ggcagcatgc
       61 taatgcagat gattgtaaat cgtttccgcc aatgccttgc tgatgccttc cactttttcc
      121 aagtcctcgc ggctggcggc aatcacgccg cgcagaccgc cgaatcgggt aagcaatgct
      181 tggcggcgtt tgctgccgat accggggatt tcgctgagtg aggaagtaac gcgggctttg
      241 tcgcgttttt tgcggtggcc ggtaatggcg aaacggtgcg attcatcgcg tacggtttgc
      301 aatagatgca aggccgggct gttgggcggc aggcggaaga cttcgccggt aaaaggcagt
      361 atgagttctt ccataccggc tttgcgctcc gggcctttgg cgataccgac caaaggaatg
      421 tgcagcccga gttcttccca taccgatacg gctacgccga tttgcccttt gccgccgtca
      481 atcaatacga catcgggcca tttgacggtc tcgccgttgg cttcggcctc ctgcattttg
      541 ccgtaacggc gcgtcaacac ttcgcgcatg gcggcgtagt cgtcgcccgg tttggcggtg
      601 gtgatgttgt agcggcggta ttgcgagggc tggatgtttt gctcatcgta cacaacgcag
      661 gacgcaatag tggcctcgcc ttgggtgtgg ctgatgtcga agcattcgag gcggttgagg
      721 ccgtctgaat ccataccgag gattttggcg agttcgtcga tgcggtgttg ctggttgctt
      781 tgttgcaggc ggcgttgtgc aatcgccatt tgtgcgtttt gttccgccat tttcaaccaa
      841 actttgcgtt cgcctatggt tttggttacg aactgcattt gtttgccgtg ttcgccttcc
      901 aaagcctctt tcagcgcatc ggggacgggg aagttgttga tgataatgtc gggtttgctt
      961 ttgcccagat agtgttgggc gacgaatgct tcggcatagt cttgcccgtt tggctcggga
     1021 tcgtttttgg tgtcggggaa gaaacttttg tctccgacgt gccgcccacc gcgtatgctg
     1081 acccagtgta cgcagacgat gccgtctgaa accgccagcg cgagcaaatc gatatcgttg
     1141 gggttgttcg ggtttttgct gtcgatgaac tggttgctct gcataatgcc gagtgcttgg
     1201 atttggtcgc ggtagcgggc ggcttcttca aattgcagat tcgcggcggc agtttgcatt
     1261 ttgtgttgca gggtgcgggt cagttcgtcg gttttgccgt tgagaaaagt ggcggcttga
     1321 cgcacgctgt cgcggtagtc ttcttcgctg atgtgtccga cgcagggcgc ggtgcagcgt
     1381 ttgatttgat acagcaggca agggcggtca cgatgctcga atacgctgtc ttcgcaggta
     1441 cgcagcataa agactttttg caggacttgg atgctgtcgc gcacggcgtt gctgttggga
     1501 tacgggccga agtattggtt tggctttttc agcgtgccgc ggtaatacgc catctgcgga
     1561 tattgatggc cgctgagcat caaataagga tagcttttgt cgtcgcggaa aaggatattg
     1621 tatttcggcg acaaggcttt gatgaggttg ttttcgagaa tcagggcttc ggcttcggaa
     1681 cgcgtgatgg tggtttcgat atgatgaacc tgttttacca tcaatgcgat gcgcggcgaa
     1741 tggtcgtttt tttggaaata gccggacacg cgccgcttga ggttgaccgc tttgccgacg
     1801 tataagactt tgccgccttc gtcaaaaaaa cggtacacgc ccggcagctt gggcaggttt
     1861 ttgaggaaga gcgggatatc gaattgttcc ggagaacggt tttctgtatt catatcaatt
     1921 atttgaaaaa cggcttcaga cggcatatcg gcagcggcag ccttatccgt tcggaaaagc
     1981 tgtcgggtgg ttatatgcgg tcaatcgatt tttacccgtt tcaggcgcaa ggcgttgctc
     2041 aacacggaaa ccgagcttgc cgccattgcc gcgcctgcga tgacagggtt taaaaaaccg
     2101 agcgcggcaa gcggaatgcc caaaatattg tagaagaagg cgaaaaacag gttttgcttg
     2161 atgtttctca acgtcgcccg cgataccgat agggcatcgg cgagttggtt gaccgaatgt
     2221 tgcatcagcg tggcggatgc ggtgtgttcg gcaacgtccg cgccgccttt catggcgaag
     2281 ctaacgttgg cggcggcgag cgcaggtgcg tcgttgatgc cgtctccgac catcgccacg
     2341 gttttgccgg cggctttgag tttctgcact tcggcggctt tgtcgcgcgg actcatatta
     2401 ccgaaggcgt gtgcgatgcc cagttgtttg gcgacgtatt cgaccgtgcc ttggttgtcg
     2461 ccgctcataa tatagacatc gatattgtgt tttttcagac ggcctatggc ttcggcggta
     2521 tcggctttca acgcgtcggc aagtgcgaat gcgccgatgg gtttgttatc gactgagacc
     2581 gcaacaatgc ttgcaatatc ccaaacgccg tctaaaaact tcggcaaggc cagttcggca
     2641 aattcggctt tgcctgcttt caccaaaccc acgccttcca cttcggcggt aatgcctgcg
     2701 ccgacaacgg tttgtgcgtt ttgtgcggcg ggaatgtcca aaccgcgcgc ttgggcggcg
     2761 gagacgatgg cacgggcgag cggatgggcg gcgttttgtt cgacggcggc ggcgatgcgg
     2821 tacaaagcgt cttcgtcaaa gccgctgtcg ggaacgcaat aaacggcggc aacctgcggg
     2881 ctgccttcgg tcagcgtacc ggttttgtcc aacacgacgg catcgacgtg ggcggcttcc
     2941 tccattgctg ccgcgtcttt aaaccaaata ccgtgtttaa ccgctttgcc cataccgacc
     3001 ataatcgcgg caggggttgc cagacccagt gcgcacgggc aggcaatcac caaaacggcg
     3061 acggcgtgca tcagcgcaac cgtccaatcg cccttaatca gccaagtaac aataaaagtc
     3121 aacaacgcaa tgcccacgac ggcaggcacg aataccgcag ccgctttatc ggctacgcgc
     3181 gcaatcggtg ctttactgcc ttgtgcttca gagagcgcgt tcatcatgtc gccgagctgg
     3241 gtttggctgc cgagctgcgt ggcgcggtac accacactgc cttcggtcat taacgcgccc
     3301 gccaacactt tgccgcccgc ctttttttct tcaggattgg attcgccggt aagatggctc
     3361 tcgtccgccc aaccgctgcc gctttcaatg atgccgtctg cggcaatgcg ttcgccgtgg
     3421 ttggcgcgga taaggtcgcc gatttgcact tggtcgatgg gaagctgttt ccattcgccg
     3481 ttgcgttgca cgttgacttg ggttggtgta agtttgagca gcaagcccaa gctgttgagg
     3541 ctggattttt tggtacggtg ttccaaaaat ttacccagtg acacaaaacc gatcaccatc
     3601 acgcccactt caaaatacac atgcgccata ccgtacgccg cgtgcgggct gaaaaacagc
     3661 atatagacgg aatacaggta aatcgagacc gtgccgatgg taacgagtac gtccatattc
     3721 gccagcccgc ctttaatgct cgcccacgcg cttttgtaaa atggcaccgc cagccaaagc
     3781 tgcaccacac tcgccaatgc aaactgcaac aagggcgaca acatccaatc atgccgtgtc
     3841 caattcagcc cttttagcat catccctacc ataccgataa ggaacgggat attgatggcc
     3901 agcaaaagcc acaacctcca gccgatatgg tgttctgctt cgggttgcgg caatgtatct
     3961 tccgtctttt ccttcgcgcc gtaaccggtt ttctcaatga ttttggcaat gtcggcagcg
     4021 gaagttttgc tgtcgtcaaa cgtaacctgc gcctcctcgc tggcgaagtt cacccccgcc
     4081 gattcgacaa agtctttttt gttcaacact ttttcaatgc gcgaagcgca ggcctggcag
     4141 gtcatgcctt cgatttggaa acggattttt tgttgcataa gcgtgattcc tgctttgata
     4201 ttcagacggc agttaaggct gcgggatgcc gtctgagcgc cggtatggtc gaaatttctc
     4261 aaacgcgccg ataattcgag tagaatccga aaacttgaca cagcaaagga aaaacaatgt
     4321 cccaacaccg caaactgatt attttggggt ccggccccgc cggatacacc gccgccgtct
     4381 atgccgcgcg tgccaattta aaccccgtca ttattacagg tatcgcgcaa ggcgggcaac
     4441 tgatgaccac caccgaagtg gacaactggc ctgccgatgc cgacggcgtg caagggccgg
     4501 aattgatggc gcggtttctc gcccacgccg aacgtttcgg aacggaaatc atttttgacc
     4561 aaatcaacgc cgtcgatctg caaaaacgcc cgtttacact caaaggcgat atgggcgagt
     4621 acacttgcga tgccctgatt gtcgcaacag gcgcgtccgc caaatacctc ggtttgccga
     4681 gtgaggaagc gtttgcaggg aaaggcgttt ccgcttgtgc cacctgcgac ggtttcttct
     4741 acaaaaacca agatgttgcc gtagttggcg gcggcaatac ggcagttgaa gaggcactct
     4801 accttgccaa tatcgccaaa accgttaccc tgatccaccg ccgcagcgag ttccgtgccg
     4861 aaaaaatcat gatcgacaaa ctgatgaaac gcgtggaaga gggcaaaatc atcctcaagc
     4921 tggaaagcaa cctgcaagaa gtactgggcg acgatcgggg cgtaaacggc gcattattaa
     4981 aaaacaacga cggttctgag caacaaattg ccgtcagcgg catttttatc gccatcgggc
     5041 acaagccgaa taccgatatt ttcaaaggtc agttggaaat ggacgaagcc ggttacctga
     5101 aaaccaaagg cggcacggcg gacaatgtcg gcgcaaccaa tatcgaaggc gtatgggcgg
     5161 cgggcgacgt aaaagaccat acctaccgtc aggcaattac cagcgcggct tccggctgtc
     5221 aggcggcttt ggacgcggaa cgctggctgg gcagccaaaa catttgaatg ccgtctgaaa
     5281 ccgaaatttt cggcggaatg caaatttttc gcaccaaatc cttgattccg tcggcaaacc
     5341 ccaatataat tcctgctctt tgaaccttgc cttctgcttt cgcatggcag aaggctgttt
     5401 ttaccatcca taaggagata acatgcgtca ttacgagatc gtgtttatcg ttcatcctga
     5461 tcaaagcgag caagtgcctg ctatggttga acgttacaaa accatgattg ccgaagcaaa
     5521 cggtaagatt caccgtttgg aagattgggg tcgccgccaa ctggcttacc cgattaacaa
     5581 aatccataaa gcccattacg ttttgatgaa catcgaaacc actcccgaag tggttgaaga
     5641 gctggaaacc gcattccgct tcaatgatgc cgtattgcgc catctaacca tcaaaaccaa
     5701 acacgccgtt accgaagcat cccctatgtt gggtggtgaa aaggctaaga acctgttgag
     5761 cggtgcgtct gaagaagcgg tcgcccaata attgggattc aataatcttg tttcgcttgc
     5821 cgcgttaatt gaaaaggttt tccctattcg atatacgcct gccggaatcc ctgttttaga
     5881 tattatttta aagcacgaat cgtggcagga ggaaaacggg cagcaatgcc ttgtccaatt
     5941 ggaaattccg gcacggattt taggcaggca ggcggaagag tggcagtatc ggcaaggtgt
     6001 atatgttcac gtcgaaggtt ttttagctca aaaaagcaga cgttccctta tgccgatgct
     6061 caggatacaa aatattcaag aatataaagg ttaaacgaca atggctcgtc aatcattcaa
     6121 acgtagaaaa ttctgccgtt tcacggctga aaaaatccaa gaagtcgatt acaaacaagt
     6181 tgatttgctg aaagacttta tctctgaaaa cggtaaaatc attcctgcac gcatcacagg
     6241 aacgaaggca ttctaccaac gccaattggc tgttgccgta aaacgcgcac gcttcctggc
     6301 tctgctgcct tacaccgacc aacacaaata attttggaga ttgaatcatg caaattattc
     6361 tgttagaaaa aatcggcggt ctgggcaact tgggcgatat cgtaaccgtt aaaaacggct
     6421 acgcccgcaa ctttctaatt cccgcaggta aggcaaaacg tgcgaccgaa gcgaatatga
     6481 aagagtttga agtacgccgc gccgaattgg aagccaaaca ggctgaaatt ttggcagatg
     6541 cccgagtccg tcaggaaaaa ttggacggtc aaaccgttac cgttgctcaa aaagccggtg
     6601 tggacggtcg cctgttcggt tccgttacca atgccgacat tgctgctgca atcgttgctg
     6661 ccggcatcga agccgtgaaa gcaaatgtac gtctgccgaa cggtcctctg aaagccgttg
     6721 gcgagtacga agtggaagtg gctttgcaca ctgatgccgt tgctaaaatt accgttgccg
     6781 tcgttgccgc aaccgagtaa ttgaaattat cagatgccgt ctgaaaccct gccgattgtc
     6841 ggaatgtggt ttcagacggt atttgttttg ttttccgtat attctgcaaa agtcgggatg
     6901 gttgttttta tagtggatta aatttaaacc agtacagcgt acctggcttc aatatactca
     6961 ataggatagg tctcttccta accgaaaaaa taattgtctt ttcccttgca aataattgaa
     7021 atcaacagat tactaaaaca cagccaaaat attcaattga caaaaacttt tctgattttg
     7081 agaaatctgt gaaaatcagg ggattattta ttttgaaaat ggtatcgggc ataaaatttg
     7141 gctgtgtttc aatatggtgc tgataacata ctttgtttca aaatgttcaa taggataact
     7201 tcctgattga ccgaaaaata atggcctttt cccttgcaag cagttgaaat caacagattg
     7261 tcaaaataca tcctaaaaat ttaataactt atatatatga ttgcaaaggt tgcggcttgt
     7321 atgccggtca ttatggcgga aataccgttt gggaggcagc cgcatcgcgt ggaatcggca
     7381 cggcaggcga tgcgccgatg ccttcgggcg tgaaggcggg gcaatcggca gtttggaaaa
     7441 aggcaatgga atctgtgggc agaagaagtt aagggtaaaa gtcgggcgca cggtcaggtt
     7501 gtctgtcctt ttgtcggaac aggagactgg gcagctttgt cctgattaat tgcttgtgca
     7561 aacggtaaaa aggttttttc gtgtgtttta aagcaggttg tacagcattt ttccggcaat
     7621 caaaagcagc ataatgccga aggatttttt gagtttggca gaagaaagtt tgtgggcggt
     7681 tttgacaccg agcggggcaa aggcaatggt tgccgcgctg aggacggcga cggcgggcag
     7741 gtaaaggaag cccagtgacc cttcgggcaa tcctgcaata ttcaggccgt tgagcagata
     7801 cgatattgcg ccggagagtg caatcggcca ggcaaggccg gatgatgtgc cgatggcttt
     7861 atgggcgggg aagccgcagt ggattaagaa ggggacggaa agtgaaccgc cgcctatgcc
     7921 gacccagctc gacattgtgc cgaacagtgt ggaaaccgca gtcagtccgg gcagcttggg
     7981 cagcgggcgg gatgccgtct gacgaccggt atgcagtgtt ttgaatgcga cggcggttaa
     8041 aaacaggatg aagaaaattt gaagcccgaa cgctgggata tattttgcgg agagtgcgcc
     8101 agcgaatacg ccgaatacca tacccggcat catcgtaaat acggttttcc agtcgaccgc
     8161 ctgttttttg tgctgcccca gcatactgga aaaggcggtg aagaccatga cggcgaagga
     8221 tgtgccgacg gcgaggtgtt gcgcgtaagg atgttgtgcc aaaccctgca aatcaagcac
     8281 ccataaaacg acagggacaa tcagcgtgcc gccgcctacg ccgaacaggc cggcaataaa
     8341 acctgccgca ctgcctacgg caagcaggat taagataatg tcccaatgcc acatttaatc
     8401 cgcctttctg tattttttcc agaatcggcg tgccgccata atgtagccgg acaggctgta
     8461 tccgagaaag aacaggaaga ggacgagcga cggttcccaa gtgaccagaa gcagcagtaa
     8521 gacggcaagc agcattccga caaaggggac ttgtctgcgg atgttgattt ctttaaaact
     8581 ccaaaaaggg atttggacaa tcatcgacag gccggcaaac agtgtgatgc ccaatgccca
     8641 ccagtggacg gcggggaatt tttcgacgct gtggttgacc caaatcagcc cgacaatcag
     8701 cgcggcggca gtcggactgg gcacgccgat aaaccagcgt ttgtccacct tgccgatgag
     8761 cgtgttgaac agcgcaaggc gcagggcggc gcaggcgcag tagatgaagg cgacggaata
     8821 accgattttg ccgaactgcc aaagctgcca tttgtaggca atcagagcgg gagcaacgcc
     8881 gaagctgacc atatcggcaa ggctgtcgag ctgctccccg aacgcgcttt ggctgttggt
     8941 cagccgcgcc acgcgcccgt ccataccgtc cagcaacata gagatgaata ccgcgatggc
     9001 ggcggtttca taacgtccgt gcatggattg ggtgattgcg taaaatgcgg aaaacagcgc
     9061 ggcgatggta aaggagttgg gcagcaggta gatactgttt tgacgcaggg agcgttgcgg
     9121 ggtgttggtc ggggtttcca tagctgaagt gtctcacgga cgtggagaat cgatagggga
     9181 ttataaccca aaccattatc gaacaatacg ccctttagga tgaagccctc ggaggcggtt
     9241 tgtttttctg atggcggatg ctttgcgggg cggtttgact ctgcctgcca ccgggtgtgt
     9301 tgtttcggat ttttgccgga tggatattgt tgccgggcat ttctgggctt gccggaaggt
     9361 atcgggggga gggcatcgga atatgcggcg caccgttcaa accggggcgg cgtttcccta
     9421 ttttcgggga gtttgcaggc ttcaacaaat tgaaagatat tttttgcatt tccgaccgta
     9481 tgctgatttt gaaacgggat aaattatagg acaaggtttt tcgcccgccg caaaggttgg
     9541 ccgcaatgcc gtctgaaaac aacggcttgc cgctaaggcg gcggtatgcg gatttgcgct
     9601 gctgaaatcg gtttgccata cccggcggtg caatctgcgg ggagggataa gccgataagg
     9661 cgggacttat cctgtttttt gtttataatg caacagaatt tggacgaacc gtcgggaatt
     9721 tatcatgatg aaaaagatac tggccgtatc ggcactatgc ctgatgactg cggcggcacg
     9781 ggctgccgat acttacggct atctcgccgt ttggcagaat ccgcagaatg cggacgatgt
     9841 tttgcaggtt aaaaccacaa aagaagattc gacgaaaagc gaagcgtttt ccgagttgga
     9901 agccttctgc aaaggtcagg acacgcttgc gggcattgct gaagacgagc cgaccggatg
     9961 ccggtcggtc gtgtcgctga acaatacctg tgtcgcgctg gcatacccga aagccttggg
    10021 cgcgctgcgt gtcgacaacg ccgtcgtgat tacttctccg cgttttacga gcgttcatca
    10081 ggtcgcactc aaccagtgca tcaaaaaata cggcgtacag ggacaatgcg gcttggaaac
    10141 agtgtattgc acatcttctt cttattacgg cggaactgtg cgctctttga ttcaaaatct
    10201 caaataaaac ggaaaatgcc gtctgaaaga tgttcagacg gcatttctat ctcgacggtc
    10261 aggattcttt cggatcgggc agcaggctgt tcaacataat ggcaagtacg gcgcacaagc
    10321 ccacgccggc aaagctgaag ctgcccaatt tgagcgtcat gccgccgatg cccgtggtca
    10381 gtaccgagct gacgatgacc aggtttttcg gcagcatcaa atcgactttg gcatcaatca
    10441 gcgttttcac gcccaaagaa gcaatcgtgc cgaacagcag cagcataatg ccgcccatta
    10501 ctggcatcgg aatggaagcc aaaaacgcat tgaatttgcc gaaaaacgcc atgcagacgg
    10561 caaaaaccgc cgcccaagtc atgatgacgg ggttgctgtt tttggtaatc atcaccgcac
    10621 ccgttacttc gccgtaggtc gtaaccggcg ggccgccgat cagacccgca acgcacacgc
    10681 ccaaaccgtc gcctgcaagg gttttgtcca agcccgggtc tttcgtatag tctttccccg
    10741 tcacattgcc gattgccatg atgccgccga tgtgttcgat ggcgggggcg acggcaacgg
    10801 gcagcataaa cagtgccgcc tgccagttga cctgaggcgt ttcaaaatgg ggaacggcga
    10861 accagggcgc gtgtgcaatg cttgccgtgt ccaccagtcc catcagcagt gccaaaacat
    10921 aacccgaagc gacaccgatc aagataggaa tcagcttcat catcctgctg ccgaaaaccg
    10981 atacgatggc ggtaacggca aaggtaaagc cggaaagaat cagtgaatcg gtatagtcga
    11041 tgacctgttt gccgtccgcc tgacccattg ccatgctgct tgccgccacg gcaacagaca
    11101 gaccgatgac catgatgacg gggccgatga cgaccggcgg cagcagtttg tgtaccgccg
    11161 ccagtccgcg ccaacgaatt agcacggcaa acacaaaata cataaagccg gcggcaaaca
    11221 gtccgaacat ggtggaaggc agcccccatt ctccgatgga gtagataatc ggtgcgataa
    11281 aggcaaacga agaaccgaga aaaatcggca ctttacgttt ggttgtgatt tggaacagca
    11341 gcgttcccaa gcctgcgccc aaaagcgcaa gagccggatt cagaccggtc agcaggggaa
    11401 ccagcaccat tgcgccgaat gccacaaata aaatctgcgc gccggaaacg gcaagtttca
    11461 gttggttcat accatcttct ttcaatcggt tcaaacggca cggattatac tggtttgccg
    11521 agggtctgcg taaaaataca tatggcgggc aacagatatg ccgtctgaaa ggcaatgtct
    11581 gtctgttgca ggatgaaagc aggagaaatg gagtataatc cgacactttc catattcaat
    11641 ccgcaagccg tccggcttcc ttatttcaga cggcattttc cggtaatacg gcatgacaga
    11701 aaaatcccat aaaaaaacag caaaaggcag ggcgggaagc ccgtccccaa cgtccgcacg
    11761 caacaagaaa gccgacaacg gcgcacgggg caataaagtt tccgagcggc tcaaggcggt
    11821 caaagagttg cagaaaaccg aaaccaaaaa ggcgcgtccc gaacatgtcg tcaaccttat
    11881 cggcgacgca ctgtggctga tgggtttggc ggcaaccctg tatttggcga tttccctgat
    11941 cagtttcgat atgggcgatc cgtcttggtc gcacagttcg ccggttgtgg aagatgtcgc
    12001 caattggggc ggactgttcg gcgcgtatgt tgccgatgtc ggctattatc ttttcggctg
    12061 gtcgttctgg tggtggatag cggctgcctg cgtcatgctg tataaaaatt ttcgtctgca
    12121 cgcaaaacag acggaaaacg aggcatacaa ccacaaaatc gctgccgccg cgctgtttgt
    12181 cctgacggtc ttcagccccg tcttggagta ttttgtgctg ggcggaaaat atgccgactc
    12241 cctgcctgtc ggagcaggcg gtatggtcgg catacgcgtc ggcgcagtgt ttgcgtggct
    12301 gctggggaaa tcgggcagcc tgctgattat cttggttgtt ctgctgttgt cgttgtccct
    12361 gctggtgcag atttcatggc tggaattttt gaacggtgcg ggcagggcgg ttcaaaaccg
    12421 cctgagtgcc ttatccggca aggtcatggc tttaggaaaa cgccggccga atagcaaaac
    12481 agacggtgtc gatacccaaa atacacggcg catggtaaaa gaagccaaga atattacggc
    12541 caaacccgtt gccttgcccg aaggcagcag cagcaaccgc aaatccgtcg cggtttccgt
    12601 cgcgccgtcg cccaaaattc aggtttctct gtttgaagat gacgaacctc ggcaggcggg
    12661 cgaataccac aagcctacat tgaacctatt gcggattcct gacagcgaac ccgtcagcat
    12721 caatcccgcc gaattggagc gcactgccga actgatcgaa tccaaactgg cagaattcgg
    12781 catcggcgta caagtcgtat ccgccacatc cggtcccgtc atcacgcgct acgaaatcga
    12841 acccgcgcaa ggtgttaaag gcagccaaat tgttgccttg tcgaaagatt tggcacgctc
    12901 tatgtcgctg caatccgtgc gtatcgtcga aaccatcgca ggtaaaaaca cgatgggcat
    12961 cgagttgccc aacgacaaac gccaagacgt gatgttgagc gaaatcttgt cctcgcccgt
    13021 gtttgccgaa gccaaatcca agctgaccgt cgcgctgggc aaagacattg ccggtacccc
    13081 cgttgtcggc gacttggcga aaatgccgca ccttttggtc gccggtatga ctggttcggg
    13141 caagtccgtc ggcgtgaacg gcatgattat gtctatgctt ttcaaagcta cgcccgacga
    13201 agtccgcttt atcatgatag acccgaaaat gcttgagttg agcatttacg acggcattcc
    13261 gcacctgctc tgtcccgtcg tgaccgatat gcgcgaagcg gggcaggcgt tgaactggtg
    13321 cgtcgccgaa atggaaaaac gctaccgcct gctttcccac gccggtgtac gcaatctgga
    13381 aggcttcaac caaaaagtcg aagccgcaaa agcggcaggc aagccgctgc tcaatccgtt
    13441 cagtctgaac cccgacagcc ccgagccttt ggaaaaactg ccgatgattg tggtcgttat
    13501 cgacgaactt gccgacctga tgatgaccga acgcaaagcc gtcgagcagc aaatcgcccg
    13561 tctcgcccaa aaagcgcgcg ccgccggtat ccatatgatt gtcgccaccc aacgtcccag
    13621 tgtcgatgtc gttactggcc tgattaaagc caacatcccg acgcgtatgg cgtttaccgt
    13681 gcaaagcaaa atcgacagcc gtaccatcct cgaccaaatg ggcgcggacg aactgctcaa
    13741 atatggcgat tcgctgttcc tccagcccgg cagtgccgaa ccgacccgcc tgcaaggcgc
    13801 gtttgtttca gacgacgaag tacatcaagt cgtcaactac gtcaaatcgc aagccccagc
    13861 cgactatatt gaaggtctgc tcagcggcga ggccgcgctg gaaactgcca atatcgttaa
    13921 tccgaatgca gacagcgacg aattgttcga tcaggcagtc gcctatgttt tggaaagcaa
    13981 aaaaacctcc atttcgtctt tgcagcggca gttgcgtatc ggctataacc gcgcggcaaa
    14041 cctgatggag gcactggaaa atgcgggtgt cgtttctccc accgacctca acggcagccg
    14101 taaaattttg gcgcacaagg accatttgta gcccgtattg caaaatgccg tctgaacggc
    14161 ggaattggcg tttcagacgg catattatgt ttcaggcgga acattgtgat atacttgcca
    14221 gctaaaatct cccctttgcg gcaatgcggt tcaaatatcc gtaccgttgc gctgtttgct
    14281 tccccatgca gggaagaagg tttaccattt tatcaacaca acaaatttaa gggcttatga
    14341 tgagcgtaac tgttgaaact ttagaaaatc tggaacgcaa agtagtgttg tccctgcctt
    14401 ggtccgaaat caacgcagaa accgataaaa aactgaaaca aacccagcgc cgtgcaaaaa
    14461 tcgacggttt ccgcccgggt aaagcacctt taaaaatgat tgcccaaatg tacggtgcga
    14521 gcgcgcaaaa cgacgtgatc aacgagctgg tgcaacgccg cttccacgat gttgccgttg
    14581 cccaagagtt gaaagtggca ggcttccccc gttttgaagg cgttgaagaa caagacgata
    14641 aagaatcttt caaagttgcc gccatttttg aagtgttccc cgaagtcgtt atcggcgatt
    14701 tgtctgcaca agaagttgaa aaagtaaccg cttccgtcgg tgatgccgaa gtggaccaaa
    14761 ccgtagaaat cctgcgcaaa caacgcaccc gcttcaacca tgtcgaacgc gaagcccgaa
    14821 acggcgaccg cgtcatcatt gactttgaag gcaaaatcga cggcgaacct tttgccggcg
    14881 gcgcatccaa aaactacgcc ttcgtattgg gcgcaagtca aatgctgcct gaatttgaag
    14941 ccggcgtagt cggcatgaag gctggcgaaa gtaaagacgt taccgtcaac ttccctgaag
    15001 aataccacgg caaagacgtt gccggtaaaa ctgccgtgtt caccattacg ctgaacaacg
    15061 tttccgaagc gactctgcct gaagtcgatg cagattttgc aaaagccttg ggtattgcgg
    15121 atggcgatgt tgccaaaatg cgtgaagaag tgaagaaaaa cgtaagccgc gaagtggaac
    15181 gccgcgtgaa cgaacaaacc aaagaatccg taatgaacgc gctgctcaaa gccgtagagt
    15241 tgaaagttcc ggttgctttg gtcaatgaag aagccgcccg tttggcaaac gagatgaaac
    15301 aaaacttcgt caaccaaggc atggccgatg ccgcgaactt ggatttgcct ttggatatgt
    15361 tcaaagaaca agccgaacgc cgcgtgtctt tgggtctgat tttggccaaa ctggttgatg
    15421 aaaacaaact ggaaccgact gaggagcaaa tcaaagccgt tgtcgccaac ttcgcagaaa
    15481 gctacgaaga tcctcaagaa gtaatcgact ggtactacgc agagccttcc cgcctgcaag
    15541 tcccgacttc tttggcgata gaaagcaacg tcgttgattt cgttttgggt aaagccaaag
    15601 taaacgaaaa agctttgtct tttgacgaag tgatgggcgc gcaagcctga ttatcctgaa
    15661 aatgccgtct tagggcagtt tttgaaagca ccgaagccgc ttttaaagcc cgctttggtg
    15721 cttttacatc atgaaaggag acgaaatgtc ttttgataac tatcttgtcc ctaccgttat
    15781 cgagcagagc ggtcgcggtg agcgtgcatt cgatatctat tcccggcttt tgaaagagcg
    15841 catcgtattc ttggtcggcc cggtaaccga cgagtccgcc aatctggtgg ttgcccaact
    15901 gttgtttttg gaaagtgaga atccggataa ggatattttc ttctatatta actcgccggg
    15961 cggttcggta acggccggta tgtcgattta cgacaccatg aatttcatca agcccgatgt
    16021 atcgactttg tgcttggggc aggcagcaag tatgggcgcg ttcttattgt cagcaggcga
    16081 aaaaggcaaa cgtttcgccc tgcccaacag ccggattatg attcaccagc ctttaatcag
    16141 cggtggtttg ggtggtcagg catccgatat tgaaattcac gcacgcgaac tgttgaaaat
    16201 caaagaaaaa cttaaccgcc tgatggcgaa acattgcggc cgcgatttgg cagatttgga
    16261 gcgcgacacc gaccgtgata atttcatgtc cgccgaagag gctaaagaat atggtctgat
    16321 tgatcaaatc ttggagaacc gcgcttcttt gcagctttaa taagaaacct gagagagaca
    16381 aatgccgtct gaacatttca gacggcattt attttataga gactgtatcg ggatgacttt
    16441 tcctttattg cgtttagaac tgttttcgcc ataattggtt ttaacatatt cctctacaat
    16501 agctaggaat tcgcttgcaa tattatcacc tttcagtgtt attttgcgct ccccatctac
    16561 ataaaccggc gcgacgggtg tttcccccgt accgggcagg ctgatgccga tgtcggccaa
    16621 tttgctttct ccgggcccat tgacaacgca gcccattacg gcaacgttca gggattcaac
    16681 cccgggataa agggtacgcc atatagacat tttttggcgc aggtaatttt gaacatcttg
    16741 tgccagctct tgaaatacgg tactggtggt acgcccgcag ccggggcagg cggtaaccat
    16801 cggcgtaaac gaacgcaatc ccatagtctg taaaatctct tgcccgacga cgacctcctg
    16861 agtacgcggg ctgccaggtt ccggagtcag tgaaatgcgg atggtgtcgc cgattccttc
    16921 ttgaagcaag acggataatg ccgccgttga tgcgacaatg cctttgctgc ccataccggc
    16981 ttcggtcaaa cccaaatgca gcggataggc gcaacggctg cccagttcgc ggtaaacctg
    17041 aatcaaatcc tgaaccgcgc tgactttgca cgacaggatg attttgtctt cgggcagtcc
    17101 caatagaacg gctttttcgg cagattccaa agcggagaca atcagtgctt ccttcgtcac
    17161 ttcttcgggc ggtttcggcg cggaagaagc gaggttggca tccatcatac gtttggcgag
    17221 gctttgatcc aaagaacccc agtttacgcc gatgcggacg gctttatcgt tttcagcagc
    17281 agtccgaatc ataaaggcaa atttttcatc gccttttacg cctttgccga cattgccggg
    17341 attgatgcgg tatttggaca atgctttgcc gcattctgga aattccgcca acaggcgttc
    17401 gccgttgaag tggaaatcgc cgataagcgg tgtggcatag cccatatcgt ccaagcggcg
    17461 gcggatttcg gcaactttgg acgcggcttc ggggctgttg acggtaatac gcaccatttc
    17521 ggatccggca tcgctcaatt ccttaatctg caatgcggtg gcttttgcat cggcagtgtc
    17581 ggtgttggtc atagattgga taacgacggg tgcttctgaa ccgacggtaa tatgatcgat
    17641 gcggacttga tgcgtcttgc ggcgttggag tgtgttcata tggtttgaat ctgtttattg
    17701 accggtgagg acggtttgca attcttccga gtaggggaaa ttcgcctgca attgtgcttc
    17761 atattcgtat gccgcctgtg cgttgccgag ggctttggca attttccagc ctagcagcaa
    17821 atcatcggcc tgaaggactt ctaccctgct ttggtatttt ttaaagtagt aatcggcatc
    17881 gcccaactgc ccggccagca ttttggtgcg cgccagttct ttaaatgcgg gtgggaactg
    17941 cggctgggcg gcgagggaac gtttcaaata ggcttccgcc aatccgaatt gcccctgttt
    18001 tgcgctgcat atgcctttat tcaggttggc aatataaggg gtcgggtagg tggggtcggc
    18061 cagggctttg tcgaaatatg ccatagattc ggcagggcgg ttgagcctgc cgcacaggaa
    18121 ccagccgtag ttgttgttga tttcggcact gtcgggtttg atggagaggg cttgccggaa
    18181 actttcctgc gccttgtcgt taactttcag gtattgatag atttcggcac ggaccagcca
    18241 ggcaagctcg tttttagggt ctgatttcaa ggcgtcttca atacttgccg tcgcctgacg
    18301 gtagtcctga ccgcgcatat attccattgc caactgggtt ttgatattgg aaacctgatt
    18361 ggctttttct gcccgcgagg ggcggtagga agtgctgcac gcgcccaagg caaggacaag
    18421 taataaagag attcgtttgg atggcttaaa aggcataatt acccctgttg tccgattaaa
    18481 atctgctgcc atttttgttg gcggcgcgtt ttatcctgaa cctgccccgc caactgtccg
    18541 caggcggcat cgatgtcgtc gccgcgcgtt tttcgtacgg taacgacaaa tcctgcctgc
    18601 tgcaaaatat cgcggaacac acggatgttc tcattgctgg agcgttcgta tccggagttt
    18661 gggaagggat tgaacggaat cagattgaac ttgcagggaa catctgtgac cagttcgatc
    18721 agttcgcgcg catgttgcgc cttatcgttt attccgtcca acatgacgta ttcgaaagtg
    18781 atgaaatccc tgggtgcttt gaccagatag cgttggcatg cggccatcaa ttctttcaag
    18841 ggatattttt tgttcaacgg tacgatttgg ttgcggactt cgtcattgga agcgtggagg
    18901 gaaaccgcca aagccaccgg catgacatcg cgcaacctgt ccatttgggg aatcataccc
    18961 gaagtggaaa cggttacgcg gcggcggctc aaaccgtagc cgtggtcgtc cagcatgatg
    19021 cttaaggcgg taacgacatt gtcgaagttc gccatcggct cgcccatgcc catcatgacg
    19081 acgttggaaa tcacgcgctc gtttttcggt gtaacgccca tcgctttgtt tgcccaccac
    19141 aattgcccga tgatttcggc agcagtcaaa ttgcggttga agccctgccg gccggtcgaa
    19201 caaaatgtac attccaaagc gcagccgact tgtgaggaaa tgcagagcgt gccgcgatcc
    19261 gattcgggga tgaagacggt ttccaccccg ttgcccgtac cgacatccaa aagccatttt
    19321 cgagtgccgt ctgaagattt ttgagacatc atcagcttgg gaatttcgat gcctgcctgt
    19381 tcgttcagtt tatggcgcaa cgattttgcc aaatcggtca tttcgttaaa attttgcgcg
    19441 ccggattggt gcatccaacg cataacctgt ttggcacgga aaggtttttc gcccatatcg
    19501 gcaaaatgtc gggtcagccc ttgaaggtcg tagttgagca gattggtttt catgtgtttg
    19561 tgtttttctt aaatcaaggc ttcaaataaa aaattgcagg gcaggcaagt ttgatttgaa
    19621 gccttttgca acagagtttt cagacggcat ggatatattt tatgccgtct gaagaggagt
    19681 tgtgcggtgt attgtatcaa cgggggcaga tttcggtttg gctgaaaaag taggcaattt
    19741 ccaaagcggc attttccgga gtgtccgagc cgtgtacggc attgatgctg accgaagtgg
    19801 caaagtccgc gcgtatcgtg ccttcggcgg cttcggcggg attggtcgcc cccatcagtt
    19861 cgcggttttt cagaacggcg ttttcgcctt ccagtacttg aatcataacc ggaccgccgg
    19921 tcataaattc aaccaatccg gcgtagaagg ggcggtcttt atgaaccgca taaaattctt
    19981 gcgcctcttt gagggtaagc tgcttcattt tggcggcaac gattttcaga ccgttctcct
    20041 caaagcggct gtatattttg ccgatgacgt ttttgccgac ggcatcgggt ttgatgatgg
    20101 agatggtacg ttcaatcgcc atgctatatc cttattttta cttaagaaga atcaaatcgg
    20161 tattctatca aaaaaataac ttatccgcct gaagccttgg gagatagggt caggtgcggc
    20221 tgttccaaat aaacttcgga ctgcatttcg accatccggc tggcggtgcg ggtaaattct
    20281 tcggcaaaat cgccttccgt atagatatgg ttgacatcta ccgcgccggt ggcacacagt
    20341 ttgacccgga aatcgtagag tacgtcaatc agccaagtca gccgccgcgc ctccgccttt
    20401 tcttgcggtg agagttgttc caaacctgaa ataaaaacca tttcataatg ttcggccaaa
    20461 tacagatagt cggactgtga gcgggggccg aagcacagtg cgcggaaatc aaaccatatg
    20521 gcacggccgg actcggcttt gtggggaatc tcccgaccgt ggatggtgct gatgccgggg
    20581 ttcaaatcgg taatgcctgt catttctttg aacagttttg ccagttttgc ctcattttct
    20641 tcattggcag gcgtaaagaa aatctcggcg gggcggaggg tacgcagtcg gtagtcttca
    20701 ccgccgtcaa cgtttaagac ggtcaggctg gactcgatga gcgcgattgt gggaagaaaa
    20761 ctgctccggt tttgaccttg cgggtagagt tcggaaggcg cgtagtttga agtcgccacc
    20821 aaaacaacgc cctcgttaag caggttttcc agcagacggc ctaaaatcat tgcatccgca
    20881 atatcgctga catgaaattc gtcaaaacac aatacgcggg tttctttggc aatctcggcg
    20941 gcaacggatt tcaacgggtt gctttcgctt ttcagggttt tcagccgctg gtggatttct
    21001 gccataaagg catgaaagtg gacgcggcgt ttgcggcggt acgggaggca gccgaaaaaa
    21061 gcgtccatca gaaagctttt gccgcgtccg accccgccat agaaataaag ccctttgggg
    21121 acttgcgggg aacgcaaact cctgcctaaa aaacggtttc ttttgcgttt gaacatcatc
    21181 aattcggtcc aaagccgatc gaggtgttcg atggcggctg cctgcgcgtc gtcgcggatg
    21241 aagttgggca gttgtgaggc agcctgatac caggtcagcg ggctgtggtt ttcaaacggc
    21301 ggggctttaa aaagttggtc tctattactc atatttaacc ttgttatact tcttgcggct
    21361 aagaaagaaa atgcttaccg tcggtattat aaatgatttc cttcaaactt ggcattttcc
    21421 gtggaaaaga ataatgacgc gcacaagggg gtataagcaa aggcggcgtt tcaatatggt
    21481 gcagatatga gctcaaatgc cttttatagt
//
LOCUS       DAXHKX010000016        20874 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHKX010000016 DAXHKX010000000
VERSION     DAXHKX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.016"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            46..1722
                     /gene="pilB"
                     /locus_tag="ACFMWZ_000528"
     CDS             46..1722
                     /gene="pilB"
                     /locus_tag="ACFMWZ_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC5934529.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(1807..2016)
                     /locus_tag="ACFMWZ_000529"
     CDS             complement(1807..2016)
                     /locus_tag="ACFMWZ_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC5934530.1"
                     /translation="MTESRQTRLQVKCPTCQTTVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSDQTESLLEISEPDGAYR"
     gene            complement(2009..2641)
                     /gene="coaE"
                     /locus_tag="ACFMWZ_000530"
     CDS             complement(2009..2641)
                     /gene="coaE"
                     /locus_tag="ACFMWZ_000530"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC5934531.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFIILIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            complement(2643..3503)
                     /locus_tag="ACFMWZ_000531"
     CDS             complement(2643..3503)
                     /locus_tag="ACFMWZ_000531"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC5934532.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            complement(3577..4809)
                     /gene="pilG"
                     /locus_tag="ACFMWZ_000532"
     CDS             complement(3577..4809)
                     /gene="pilG"
                     /locus_tag="ACFMWZ_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC5934533.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            5166..6809
                     /gene="pgi"
                     /locus_tag="ACFMWZ_000533"
     CDS             5166..6809
                     /gene="pgi"
                     /locus_tag="ACFMWZ_000533"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC5934534.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDEVLCRLNPETTVFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETGCHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLERLTPYNLGMLMAAYEHKTFVQGVIWDINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVRDASTEGLMAFYRECRLKGGGAA"
     gene            6779..7009
                     /locus_tag="ACFMWZ_000534"
     CDS             6779..7009
                     /locus_tag="ACFMWZ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934535.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            7019..7840
                     /gene="dapD"
                     /locus_tag="ACFMWZ_000535"
     CDS             7019..7840
                     /gene="dapD"
                     /locus_tag="ACFMWZ_000535"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC5934536.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            7995..8780
                     /gene="fabI"
                     /locus_tag="ACFMWZ_000536"
     CDS             7995..8780
                     /gene="fabI"
                     /locus_tag="ACFMWZ_000536"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC5934537.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGADLAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(9024..10022)
                     /gene="ilvE"
                     /locus_tag="ACFMWZ_000537"
     CDS             complement(9024..10022)
                     /gene="ilvE"
                     /locus_tag="ACFMWZ_000537"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC5934538.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            10332..10646
                     /locus_tag="ACFMWZ_000538"
     CDS             10332..10646
                     /locus_tag="ACFMWZ_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC5934539.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQSVNLPLIVI
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            10657..11826
                     /gene="lapB"
                     /locus_tag="ACFMWZ_000539"
     CDS             10657..11826
                     /gene="lapB"
                     /locus_tag="ACFMWZ_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC5934540.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            11851..12267
                     /gene="gloA"
                     /locus_tag="ACFMWZ_000540"
     CDS             11851..12267
                     /gene="gloA"
                     /locus_tag="ACFMWZ_000540"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC5934541.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(12440..15064)
                     /gene="tspA"
                     /locus_tag="ACFMWZ_000541"
     CDS             complement(12440..15064)
                     /gene="tspA"
                     /locus_tag="ACFMWZ_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC5934542.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTETVA
                     ETSDFHTAADDLSALLQPAEAPAVEENVTKTVAEIPDFNATADDLSALLQPSEVPAVE
                     ENAAETVADDLSALLQPAEVPAVEENAAEITLETPDSNTSEADALPDFLKDGEEETVD
                     WSIYLSEENIPNNADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEE
                     AEGDVLKRAQALAQELGI"
     gene            15372..15902
                     /locus_tag="ACFMWZ_000542"
     CDS             15372..15902
                     /locus_tag="ACFMWZ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC5934543.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLSDAVWAKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            15905..16195
                     /locus_tag="ACFMWZ_000543"
     CDS             15905..16195
                     /locus_tag="ACFMWZ_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC5934544.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            16195..16473
                     /locus_tag="ACFMWZ_000544"
     CDS             16195..16473
                     /locus_tag="ACFMWZ_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC5934545.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            16522..17388
                     /locus_tag="ACFMWZ_000545"
     CDS             16522..17388
                     /locus_tag="ACFMWZ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC5934546.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            17459..18217
                     /locus_tag="ACFMWZ_000546"
     CDS             17459..18217
                     /locus_tag="ACFMWZ_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC5934547.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDT
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            18291..18782
                     /gene="dtd"
                     /locus_tag="ACFMWZ_000547"
     CDS             18291..18782
                     /gene="dtd"
                     /locus_tag="ACFMWZ_000547"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC5934548.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            18813..19823
                     /gene="dusA"
                     /locus_tag="ACFMWZ_000548"
     CDS             18813..19823
                     /gene="dusA"
                     /locus_tag="ACFMWZ_000548"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC5934549.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(19893..20780)
                     /gene="gluQRS"
                     /locus_tag="ACFMWZ_000549"
     CDS             complement(19893..20780)
                     /gene="gluQRS"
                     /locus_tag="ACFMWZ_000549"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC5934550.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQTGARRGADGFVYNGRCRNPQQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     AGDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITAP"
BASE COUNT         4718 a         5347 c         5974 g         4835 t
ORIGIN      
        1 aagctgtaca ggtataacaa tgaataaatt tggggataag gtcgtatgag cgtaggtttg
       61 ctgaggattc tggttcaaaa ccaggtggtt actgttgagc aggccgagca ttactacaat
      121 gagtcgcagg cgggtaagga agtgttgccg atgctgtttt cagacggtgt catttcgccc
      181 aagtcgcttg cggcattgat tgcgagggtg ttcagttatt cgattcttga tttgcgtcat
      241 tatccgcgcc acagggtgct gatgggggtg ttgacggagg agcagatggt ggagttccac
      301 tgtgtgccgg ttttccgtcg gggcgacaaa gtattttttg cggtttccga tccgacacag
      361 atgccgcaaa ttcagaaaac cgtttctgcc gcagggattg aggttgaatt ggtcattgtc
      421 gaggatgacc agttggcggg tttgctcgat tgggtgggtt cgcgttcgac atcgctgctt
      481 caggagcttg gggaggggca ggaggaagag gaaagccaca ccctgtatat cgacaacgag
      541 gaggcagaag acggccctgt tccgaggttt atccataaga ctttgtcgga tgccttgcgc
      601 agcggggcat cggacatcca tttcgagttt tacgaacaca atgcccgtat ccgtttccgt
      661 gtggacgggc agctccgcga ggtggttcag ccgcccattg cggtaagggg gcagcttgct
      721 tcccggatta aggtaatgtc gcgtttggac atttccgaaa aacggatacc gcaggacggc
      781 aggatgcagc tgacctttca aaagggcggc aagcctgtcg atttccgtgt cagcacattg
      841 ccgacgctgt ttggcgaaaa ggttgtgatg cggattttga attccgatgc cgcgtctttg
      901 aacatcgacc agctcggttt tgagccgttt cagaaaaaat tgttgttgga ggcgattcac
      961 cgtccttacg ggatggtgct ggtaaccggc ccgacgggtt cgggtaagac ggtgtcgctc
     1021 tatacctgtt tgaatatttt gaatacggag tcggtaaata ttgcaacggc ggaagaccct
     1081 gccgagatta acctgccggg catcaatcag gttaacgtca atgataagca gggcctgact
     1141 tttgccgctg ctttgaagtc tttcctgcgt caggacccgg acatcattat ggtcggtgag
     1201 attcgtgatt tggaaactgc cgatattgcg attaaggcgg cacaaacagg gcatatggtg
     1261 ttttccaccc tgcacaccaa taatgcgccg gcgacgttgt cgcgtatgct gaatatgggt
     1321 gtcgcgccgt ttaatattgc cagttcggtc agcctgatta tggcgcagcg tcttttacgc
     1381 aggctgtgtt cgagctgcaa acaggaagtg gaacgcccgt ctgcctctgc tttgaaggaa
     1441 gtcggcttca ccgatgagga ccttgcaaaa gattggaaac tttaccgcgc cgtcggttgc
     1501 gaccgttgcc gggggcaggg ttataaaggg cgtgcgggcg tgtatgaggt tatgcccatc
     1561 agcgaagaaa tgcagcgcgt gattatgaac aacggtacgg aagtggatat tttggacgtt
     1621 gcctataagg agggtatggt ggatttgcgc cgggccggta ttttgaaagt tatgcagggc
     1681 attacctcgt tggaagaggt aacggcaaat accaacgatt aggcttggga atgaaaatgc
     1741 cgtctgaagc gtgtttgttt cagacggcat ttgattttca ggggtttgcc ggagggcggg
     1801 gtgcggtcag cggtatgcgc cgtccggttc ggatatttcc agcaaacttt ccgtttggtc
     1861 ggaaaccgta tatttcccgt ctgcccatcc gcccaagtcg atcagtttgc agcgttgcga
     1921 acagaacggg cggaatgcgt tttcgggttt ccatactact gtcgtttgac aggtcggaca
     1981 tttgacttga aggcgtgttt gccgcgattc agtcattgtg ttttccttgt gttggttttg
     2041 aggcgaaaat ccctgaataa aacgcgtgca ggcgcattgt tttctcacgc aggcttttga
     2101 ggctgccgtc attgagcagc acatcgtctg caagcagcag gcgttcggat tcggatgcct
     2161 gatggctgat gacggcagcc acctcgccgc gcgtcagccc gctgcgggcc atcaccctgc
     2221 cgatacgttt ttccacaggg gcacttatgg tcaggacacg ccgtatcagg atgataaatt
     2281 gacgcttttc cgtcagcagc ggaatttcga caatgccgta agccgcatcg gtaaaggttt
     2341 cttgctgttt tttgatttct gagaaaatca gcggcaacat cacggattcg agcaaggctt
     2401 ttcgcgatgg ggaggcaaag acttctttac gcaatatgtc gcgtcgcaac aaaccctgtg
     2461 tgtcgaaaac ggtgtcgccg aacagccgcc tgatttccgg cagggcgatg ccgtctgaag
     2521 ccgtcagcga gtgcgccgcc gcatccgcat cgatacgcgg cacgcccaaa tcggcaaaac
     2581 attgcgcggc tgccgatttg ccgctgccga ttccgccggt cagcccgacc cataccgtca
     2641 tcttacagca ccggatgggt cagccaccag ttgaccgccc gccatacgga atcgtttgcc
     2701 gtaaaaatta tccagcccga aactgtcagt gcggggccga aggcaaaatg ctgccccttg
     2761 gcgacgcgca taacgattgc cgcgaccaaa ccgatcagcg aggaaacaaa aatcagcacg
     2821 ggcaatgcgg atatgccgag ccacgcgccc aatgcggcaa tcagtttgaa atctccgttg
     2881 cccataccga tttcgccttt aatcagttta tataccgcgc ataaaagcca tatcgaacca
     2941 tagccggcaa ccgcacctaa aacggcagac tgcaaaggca cgaagccgcc gtccaaatta
     3001 aatatcagcc ccagccaaat caagggtaat gtcatcgagt cgggcaggta ttgggtgtcc
     3061 gcatcgataa aggtcaggga aatcagaaac gcggtcagta tcaaaccgcc caatgtaatc
     3121 caagaccagc cgtattgcca ggcaaccagc ccgaacaata cgccggtcag cagctcgatt
     3181 aagggataac gtatgctgat tttggtttgg caggaagcgc atttgccgcg caggagcagg
     3241 tagctgacaa tcgggatgtt ctgccacgcg cgtatcggca cgcggcattt gggacagcag
     3301 gaatccggtt tcatcaggtt gaaggtacgg ctttcctctt cggtcagcgg caggtttaaa
     3361 tattctttgg caaataccgt ccagccgcgt tccatcatga ccggcacgcg gtaaatgacg
     3421 acgtttaaga aactgccgac cagcagcccg aacaccgctg ccaaaggcac ggcaaacggc
     3481 gacaatacag acaaatcaga catattttgt tctcaatgta ttcaaaacaa aaacaaaccg
     3541 gcgcagagcg aatccgcgcc ggatctgtgc ggcaaatcag gcgaccacgt tgcccaaatt
     3601 aaacagcggc agatacatgg cgaccagaag cgtgccgatg accaagccta aaatcacgat
     3661 aatgatcggc tccatcatag cggacagcct gccgaccgca ttgtccacct cgtcttcgta
     3721 aaattcggcg gctttgttga gcatatcgtc caaagaaccc gattcctcgc cgatggaaga
     3781 catctgcaac atcatattgg ggaacagttc cgtcgcacgc atccccgaag tcatagacaa
     3841 accttggatg acgcgcgtac ggatttcccg ggtggcttct tcatagatta aattgcccgc
     3901 cgcgccggca gtggagtcca atacatcgac caaaggcacg cctgccgcaa tcagcgtcgc
     3961 cgtcgtcctg ccccagcggg caatcgtccc tttgcggaca atgtctccga aaatcggcat
     4021 acgcagcagt atggcatcca tacgccgttg gattttaatc gaacgcgcct tcaatttaag
     4081 gaagccgtat atggcaaagc ccagtgcgat cagcaccatc cagccgtatg agacgaaaaa
     4141 gtcggacata tccatcactg tttgggtcag tgcgggaagc tccgcgccca tattggcgta
     4201 aacttcttta aaggcgggca gtacgaaaat catcatcacg aataccaaac cgatggcgac
     4261 ggcgatgacg gataccggat aggtcagcgc ggtttttacc tttttgcgga tggcctgggt
     4321 tttttctttg taaattgcca atttgtccag caggctttcc aatacgccgc ccgtttcgcc
     4381 cgccgcaacc aggttgcagt agaagcggtc gaaatatttc gggtggtttg agaatgcgcg
     4441 gctcaacgag ctgccctgtt ccacttcgcc tcggatttcc atcagcattt ccgtcataga
     4501 cgggttgccg tgtccgcgcg ccacgatttc aaatgcctgc atcagcggca ggcccgcttt
     4561 aatcatcgtg gaaagctggc gggtgaaaac ggtgatgtct tcttgtgtga ttttgcgctt
     4621 ggagcttgtt ttcacacggg taatctgcaa cgggcggatg ccgcgttttg ccagtttttt
     4681 gcgcgcctct tcttcggtaa acgcggatac ttcgccgttg accagtttgt cggaggcgga
     4741 atgcctgcct tcaaagataa agcgtttttc tttctttgcg aacaaagaaa atcctccgtt
     4801 tttagccata ttctagcccc gtaaagtaat tggaataaaa tgtaagaaac atcgtgaaaa
     4861 aacagtaccg gcgtgttccc ggtaagatga aaaccgccga catcccgcct gcgggcggca
     4921 aacgggacag aatcggatgc gattatacct tatttaggcg gctgtccggc atttatgcgt
     4981 acacaataaa tcttgcagga tattgttgcg ggtcaaatgc cggccggagg gcatttccgc
     5041 catatggaaa taaggtgcta ttggacgcgg cgggcggtgt tccggagatt cgccaaagcc
     5101 gctgccgttt gttaaactac attctgctac attttaatcc ggttctgaaa aatcaaggaa
     5161 aacagatgaa tgcttttacc cgtgcatggt atgcgctcga acgccattat caggatacgc
     5221 gtcatgtcct tttgcgcgac cgctttgcct gcgaacccga ccgttttgag cgtatgcatg
     5281 agcgtttgga cgggatgttg ttcgattaca gcaaaaaccg tttgggcgaa gatacgctgc
     5341 aactgctctg ccgtcttgcg gagacggcgg atttggaagg gaaaatgcgt gctttgcgga
     5401 cgggtgcgaa ggtcaacaac agtgaggggc gtgccgcgct gcatacggct ttgcgcctgc
     5461 ccgacggtgc gggtgccgtt tatgcggacg gcagggacgt gttgcccgaa atccgccgcg
     5521 agttaaatcg tgcgttgaag tttgcacaca gtttggacga cggttcgtat caggggacaa
     5581 ccggaaaacg gattacggat tttgtccaca tcggcatagg cggatccgac ctcgggccgg
     5641 caatgtgcgt gcaggcactt gagccgttca gacggcatat cgccgtccat tttgccgcca
     5701 acgccgatcc tgcctgcctg gatgaggtct tatgccgtct gaaccccgaa acgacggtgt
     5761 tttgcgttgc cagcaagtcc ttcaaaacgc ctgaaaccct gctcaatgca gaggcggtca
     5821 aggcttggta tcgtggtgcg gggttctcgg aatcggaaac gggatgtcat ttttgcgcgg
     5881 tatctgccga tacggaagcg gcgcaaagtt tcggcattgc ggcggaacgc gtgtttgcga
     5941 tgtacgactg ggtgggcgga cgctattccg tctggtcgcc cgtcggtttg cccgtgatgg
     6001 ttgcggtcgg cggggcgcgt ttccgcgagt tgttggcggg ggcgcacgcg atggacagcc
     6061 attttttcca cacgccgccg cgccgcaaca ttcccgttct gatggcactg attgccgtgt
     6121 ggtataacaa tttccaacac gcggacgggc agaccgccgt tccgtacagc cacaacctgc
     6181 gcctgctgcc ggtgtggctg aaccagctcg atatggagag tttgggcaaa agccgcgctt
     6241 cagacggcag tcccgccgtg tgcaaaacgg gcggcatcgt gttcggcggt gaaggggtca
     6301 actgccagca cgcctatttc caactgctcc accaaggcac acgcctgatt ccctgcgact
     6361 ttatcgtccc gatgacggcg cagggcgtgg aggacggacg cagccgtttt accgttgcca
     6421 acgcctttgc ccaagcggaa gccctgatga agggcaaaac cttggacgaa gcacgcgccg
     6481 aactggcaga tttgcccgaa gcggaacgcg aacgcctcgc gccgcacaaa gaattccccg
     6541 gcaaccgccc cagcaacagc atcctgctcg aacgcctcac gccctacaat ctgggtatgc
     6601 tgatggcggc ttacgaacac aaaacctttg tgcagggtgt aatttgggac atcaatccct
     6661 tcgatcagtg gggggtggaa tacggcaaac agttggcgaa aaccatcatc ggcgaactgg
     6721 aaggcggcac gtccgtacgc gatgcctcga ccgaagggct gatggcgttt taccgtgaat
     6781 gccgtctgaa aggcggcggc gcggcataaa agtaccgccg ccgttctgta ttgattcggg
     6841 tgcggaaaag gcaatacctg ccgcccgccc gattccgaaa cgccaatgtt cggcaaccgc
     6901 ccgcgtattg ctgacgaata tgcgtttgcg tggcacaata gcgcattcat ttcaaatgaa
     6961 catactgctt gaaaataccg gcaagcgtcc cacgaaacat ctcacataag gaaacattat
     7021 gtctttgcaa aacatcatcg aaaccgcctt tgaaaaccgc gcggacatca ccccgaccac
     7081 cgttactccc gaagtcaaag aagccgtgtt ggaaaccatc cgccagctcg attccggcaa
     7141 attgcgcgtc gccgaacgct tgggcgtggg cgagtggaaa gtcaacgaat gggcgaaaaa
     7201 agccgtgttg ctgtccttcc gcatccaaga caacgaagtc ctcaacgacg gcgtgaacaa
     7261 atacttcgac aaagtgccga ccaagtttgc cgactggtct gaagacgagt tcaaaaacgc
     7321 aggcttccgc gccgttccgg gtgcggttgc ccgacgcggc agctttgtgg cgaaaaatgt
     7381 cgtgctgatg ccgtcttatg tcaacatcgg cgcatacgtc gacgaaggcg cgatggtcga
     7441 tacttgggcg actgtcggct cttgcgcgca aatcggcaaa aacgtgcact tgagtggcgg
     7501 cgtcggtatc ggcggcgtac tcgaacccct gcaggccgcc cccaccatca ttgaagacaa
     7561 ctgcttcatc ggtgcgcgtt ctgaaatcgt tgagggtgtg attgtcgaag aaggcagcgt
     7621 gatttcgatg ggcgtgttca tcggtcaatc caccaaaatc ttcgaccgca ccacaggcga
     7681 aatctaccaa ggccgcgtac cggcaggttc ggttgtcgta tccggcagta tgccttccaa
     7741 agacggcagc cacagccttt actgcgccgt catcgtcaaa cgtgtggacg cgcaaacacg
     7801 cgccaaaacc agtgtgaacg aattgttgcg cggcatctga tgccttaaac cgtatttgaa
     7861 acgtccgatg ccgtctgaaa tccgcttcgg acggcattgc cgtttgcacg ctgcaacgtg
     7921 aaaacacaga aatagggaca atttgctata atcaacggtt tagaacgaac cgaacactat
     7981 ttgaaggata caaaatgggc tttctgcaag gcaaaaaaat tctgattacc ggcatgattt
     8041 ccgagcgttc catcgcttac ggcatcgcca aagcctgccg cgaacaaggc gcggatttgg
     8101 cgtttaccta cgttgtggac aaactggaag agcgcgtccg caaaatggcg gcggaattgg
     8161 attccgaact cgtattccgc tgcgatgtcg ccagcgacga cgaaatcaac caagtgttcg
     8221 ccgatttggg caaacattgg gacggcttgg acggtttggt gcattccatc ggttttgcgc
     8281 cgaaagaagc cttgagcggc gacttcctcg atagcatcag ccgcgaagcg ttcaacaccg
     8341 cacacgaaat ttccgcatac agcctgcccg cattggcaaa agccgcccgt ccgatgatgc
     8401 gcggcagaaa ctccgccatc gttgccctga gctacttggg cgcggtgcgc gcgattccga
     8461 attacaacgt gatgggtatg gcaaaagcca gccttgaggc gggcatccgt tttaccgccg
     8521 cctgtctggg caaagaaggc atccgctgca acggcatttc cgccggtccg atcaaaacac
     8581 tcgccgcctc cggcatcgcc gatttcggca aacttttggg acacgtcgcc gcccacaatc
     8641 cgctgcgccg caacgttacc attgaagaag tcggcaatac cgccgccttc ctgctgtccg
     8701 acctgtcgtc cggcattacc ggcgaaatca cttacgttga cggcggttac agcatcaatg
     8761 ccttgagcac cgagggataa tccgccgttt tcaaatccgt gcgccgtccg tgccgcatat
     8821 cggtttcggg cggcgttttg ccgtctgaag cgtatttcta gggaaatgcc cgacttacgg
     8881 caggcgggat gggaaatgcg gacgcttgtt ttaaccgatt gcctttgtgc cgacttgctg
     8941 caggtgcagc ggaaacggtt cggatgcgaa aatgccgtct gaaacgccaa acgggtttca
     9001 gacggcattt tttatttaaa gcatcagcac acttcaacca gccagccgta tttgtcttcc
     9061 gccaaaccat actggatgtc ggtaatcgcc ttacggatgg catagccgcg ttcttggctt
     9121 ttcacttcga tttctttgcc gccgatgacg aaggaagtaa cgggcgagat gacggctgcc
     9181 gtaccggtca gaatcgcctc cgcaccgttt tccaccgcag ctttgagttc gtcaaccgtg
     9241 aaattgcgtt cgctgacggt atagcccaaa tctttggcaa ccgtcagtac ggaatcgcgg
     9301 gttacgccgt gcaaaaattc gtcggtcagc ggtttggtaa tgatttcatc gccgttaatc
     9361 aggataaagt tggacgcgcc ggtttcctgc acgtcgccgt tcgggcagaa gaggacttgg
     9421 tttgcgccgt attcggcttt cgccttcagc acccagtgca tggcggaagc gtagttgccg
     9481 ccgcatttga cgcggcccat atgcggggcg cagcggatgt gttcggtttc cactaaaatt
     9541 ttgacgggcg agccgacttt gaaatagtcg ccgacggggg aagccaaaat atacagcagg
     9601 gcggtttcgg aaggagaacc ggccttgccg atgacgggat cggtaccgat taaggtcggg
     9661 cgcaggtaca gggcggcagg cgcatcggga atttcgtcgg cggcacgttt gaccagttcg
     9721 accaaagcat cgagataggc ttgggtttcg gggcggggca ggtgcaaaat gtccgcactt
     9781 tgccgcatac gcgcgatatt ggcagtcgga cggaacagta cgattttacc gtctgcctga
     9841 cggaaggctt tcagtccctc gaaacattcg ctgccgtagt gcagggcgtg cgcacccggt
     9901 acgagggaga ggtcttggga agattgccat tcggtcggct gccatttgcc ttcgcggtag
     9961 gcgaggacgg gcatttgact gtgaaaaacg ctgccgaata cggcgggtac gggtctgctc
    10021 atgatgtaaa gcctttctta ttctgatatg tttcaatgaa cggtttgaat ttgaagattg
    10081 taaagatacg cctgcaaaca gggttttgac aagtgcgcgg cgggtttttc tgtcgatgcg
    10141 gtgtccaatc cgttattttt caaatggaaa ggaacggtgt atttggtaaa attgtcggca
    10201 atcgcatact ccgtatgtcg tccgaacacg ctgccgcatc ctatccgaaa ccgtgcaaat
    10261 cgtttaaact agcgcaatct tggttcagag tgcgaagctg tccgggcggc gtttttattt
    10321 acggagcaaa catgaaactt atctataccg tcatcaaaat cattatcctg ctgctcttcc
    10381 tgctgcttgc cgtcattaat acggatgccg ttaccttttc ctatcttccg gggcagagtg
    10441 tcaatctgcc gctgattgtc atattgtttg gcgcatttgt agtcggtatt gtttttggaa
    10501 tgtttgcctt gttcggacgg ttgttgtcgt tacgtggcga gaacggcagg ttacgtgccg
    10561 aagtaaagaa aaatgcgcgt ttgacgggga aggagctgac cgcaccaccg gcgcaaaatg
    10621 cgcccgaatc tgccaaacag ccttgagaaa gccgatatgg acaatgaatt gtggattatc
    10681 ctgctgccga ttatactttt gcccgttttc ttcgcgatgg gctggtttgc cgcccgtgtg
    10741 gatatgaaga ctgtattaaa gcaggcaaaa agcataccgt cgggatttta taaaagtctg
    10801 gatgccttgg ttgaccgcaa cagcggacgt gccgccaggg aattggcaga ggtcatcgat
    10861 cagcagcctc agtcatacga cctgaacctc accctaggca agctttaccg tcagcgtggc
    10921 gaaaacgaca aagccatcaa tatgcaccaa acattgcttg actctcccga tacaaccgga
    10981 gccaagcgtg cgcgcgtatt atttgaattg gcgcaaaact atcaaagtgc ggggttggtt
    11041 gaccgtgccg aacagatttt tttggggctg caagacggtg aaatggcgcg tgaagccaga
    11101 cagcacctgc tcaatatcta ccaacaggac agggattggg aaaaagcggt tgaaaccgcc
    11161 cggctgctca gccatgacga gcagacctat cagtttgaga ttgcacagtt ctattgcgaa
    11221 cttgcccaag ccgcgctgtt caagtccaat ttcgatgccg cgcgtttcaa tgtcggcaag
    11281 gcactcgaag ccaacaaaaa atgcacccgc gccaacatga ttttgggcga catcgaacac
    11341 cgacaaggca atttccctgc cgccgtcgaa gcctatgccg ccatcgagca gcaaaaccat
    11401 gcatacttga gcatggtcgg cgagaagctt tacgaagcct atgccgcgca gggaaaacct
    11461 gaagaaggct tgaaccgtct gacaggatat atgcagacgt ttcccgaact tgacctgatc
    11521 aatgtcgtgt acgagaaatc cctgctgctt aagggcgaga aagaagccgc gcaaaccgcc
    11581 gtcgagcttg tccgccgcaa gcccgacctt aacggcgtgt accgcctgct cggtttgaaa
    11641 ctcagcgata tgaatccggc ttggaaagcc gatgccgaca tgatgcgttc ggttatcgga
    11701 cggcagctac agcgcagcgt gatgtaccgt tgccgcaact gccacttcaa atcccaagtc
    11761 tttttctggc actgccccgc ctgcaacaaa tggcagacgt ttaccccgaa taaaatcgaa
    11821 gtttaaccac caccgaaagg aacacaaaaa atgcgcttac tccatactat gctccgcgtg
    11881 ggcaatctcg aaaaatccct cgatttctac caaaacgttt tgggtatgaa actgctccgc
    11941 cgaaaagatt atcccgaagg cagatttacc cttgccttcg tcggttacgg cgatgaaacc
    12001 gacagcacgg ttttggaact gacgcacaac tgggatacgg aacgatacga cttgggcaac
    12061 gcctacggac acatcgcggt tgaagtggac gatgcctacg aagcctgcga acgtgtgaag
    12121 cggcagggcg gaaacgtcgt ccgcgaagcc ggcccgatga aacacggcac aaccgtgata
    12181 gccttcgtcg aagaccccga cggatacaaa atcgagttca ttcaaaagaa aagcggtgac
    12241 gattcggttg cctatcaaac tgcctgatac cgccgccgcc aatgccgtct gaagccttta
    12301 ggggtttcag acggcatttt gttgccgtcg gcctgctgtt tgagcctgtg ccggttcaaa
    12361 ctttatccgt tacaccgata aggcaaaaaa agatgccgtc tgaaacggca tccttgatct
    12421 gcgaaagggc agttgggaat caaataccca attcctgcgc caatgcttgg gcacgtttga
    12481 gtacgtcgcc ttccgcttct tccagcaatt tctgcactgt ctcggcagcg gcatcgcggt
    12541 cgccgatttc gagatacatt tcggcaaggt cgtatttcgc ttcggaaggc gcgtcagaac
    12601 ctacagattc cgaagggaaa ctggtatctg cattatttgg gatattttct tccgagaggt
    12661 agatgctcca atctaccgtt tcctcctcgc cgtctttcag gaagtcgggc aaagcgtctg
    12721 cctcagaggt gttggaatca ggcgtttcca aagtgatttc cgctgcattt tcctcaacgg
    12781 caggtacttc agcaggttgc aacagtgcgg acaaatcatc ggcaacggtt tccgctgcat
    12841 tttcctcaac ggccggtact tcagaaggtt gaagtaatgc ggacaaatcg tctgcggtgg
    12901 cgttgaaatc aggtatttcg gcaacggttt tcgttacatt ttcctcaacg gccggtgctt
    12961 cagcaggttg caacagtgcg gacaaatcgt ctgcggcggt gtggaagtcg gacgtttcgg
    13021 caacagtttc cgttacattt tcctcaacag ccggtgcttc agcaggttgc aacagtgcgg
    13081 acaaatcatc ggcaacgatt tccgctgcat tttcctcaac ggcaggtact tcagaaggtt
    13141 gaagtaatgc ggacaaatcg tctgcggtgg cgttgaaatc gggtgtttcg gcaacggttt
    13201 ccgttacatt ttcctcaacg gacggtgctt cggcaggttg aagcaatgcg gacaaatcgt
    13261 ctgcggcggc gttgaaatcg ggcgtttcag gcgcagtttc cgcgacggca tcggtttcgt
    13321 acactttcag gaaatcgtgc aactcttccg gtgtttggac ttcggcaact gttttttcca
    13381 agatggtttc gggcgaggaa gccttcagga agcctgccag tccggagggt gaggcaggtt
    13441 ttgcggaagc tgtttcttct gtgccgatat ggttgtttga gggcaggttg tcggagaaat
    13501 cggtatcgac ggtttccggt ttgttttcgg cagtttgggc gacagattcc ggttcgggcg
    13561 tgtcgatgac gatttcgaca gggttgtacg ggttgaaggt ctcgggctcg tacacgctgt
    13621 ctgtggattc gatggcgttc caatcggcat ccgcgcgttt ttgggtttct tcatcctgcg
    13681 taagtgcgcc ggataaaatg ccgttttgcg cggctgccag gctgtcgaaa tccaagtcga
    13741 tgcggttgga aggcgtatcg gtttcgacat cgaacgtttg ttttgccgat aactcttctt
    13801 cagattcccc atctaaggca agtgtgtcgt ttacatcgtt tttcggagcg ggttcgggcg
    13861 ttgccggagt ttcgacttcg gcaaaggtga tttctatgcc gtcgtctgcc gcgtcgtcaa
    13921 ggtcaggctc ttcctcaggg acggattctt cggtacggcg cgcgcgtttg gattgggcaa
    13981 ggcgcaaaag cagcagcagg gcgatcaatg ccgcgcctcc gccggcaagc agcaaggtgt
    14041 acgaaccgcc gaacagaccg tcaaacagtc cgctttcggt ttcttcttcg gcagaaacct
    14101 gttcgacagg ttcggaaacg gcgttaccgg tttcgtcggt cggcgtgtcg atggcagaag
    14161 cggcggcttc ttggggggcg gattcggcag cggtttccga tgcggcagta tttgcagcgg
    14221 gtacaggttc gggtcgaacg gccggttttt ccgcttttgc ttcgggcgcg gcaacttttg
    14281 cttcaggttt ttcaaccggt ttctctaccg ttgcctgttt ggacggttcg gacggcatgg
    14341 atgcggtttc ggctttgggt ttcgccgttt gcggtttggg ttgttccgct ttgatcctgt
    14401 tcagattcgg aatgtgaagc acgctgcccg cacgcagtct gccgtgtgcg gaaacatttg
    14461 ggtttgcctt cagcagcgca tcggcaacct gttcgagcgt caggtgtttc gggcggatgg
    14521 cggcggcaat ctgtttgacc gtttcgcctt tgcggacggt atgggttttg ccgttgtatg
    14581 ccggtttgac ggctgcgttc gcgctgtctt ttttatcggt tttgcggagg gctttggcgt
    14641 tttgattttc ttgggactct gctgtcggag cggttttgcg gtgtgtcttg ccgtctgaaa
    14701 gtgcagattt ggttttgggc gagtagccga caggatcgag gatggcggtg tattcgcgta
    14761 cctgtgcgcc tgcgccgatg cggaacacca ggacgggatc gcggactgcc tgttcggaag
    14821 aaacggcaat gacggctttg tcgcccaact tgtggacttt ggcggtcagg cctttttcgg
    14881 aaacggtaac gctgccgccg cctagcaggg ctttggcttc ttcgccggtt acggtaatgc
    14941 tgccggaaaa gggttcgtca aggttggact ggatattcag tccgcccagt ccagcatgtg
    15001 cctgaaagga tgcggcaact gcgacggagg cggcaatcag tttgatttgt ctgttgtttt
    15061 tcaagatgta tcccctgtgg gttggcggct gaatacggtt tgaccgcgta cagtctgtaa
    15121 atttcgtcat catcgggcat cggcggggca gtcggccggc gggcatttaa tatgtgaatg
    15181 taccgaccgc cgccacattt taaacggcaa tcattcgccg tttttacaaa ttatgacata
    15241 tctccatctt ttttcaaaaa catctgtgca tatttgcatc aatcaaaaca aaatttgttg
    15301 gttttgcagg tgcaaaaaca gggttctgcc tgtatgatta gcgtttattt gatttgcttt
    15361 ctcatttgga tatgaaattc gtcagcgacc ttttgtccgt catcctgttt ttcgccacct
    15421 ataccgttac caaaaacatg attgccgcaa cggcggtcgc attggttgcc ggtgtggttc
    15481 aggcggcttt tctgtattgg aaatataaaa agctggatac gatgcagtgg gtcggattgg
    15541 tgctgattgt ggtattcggc ggcgcaacca ttgttttggg cgacagccgc ttcattatgt
    15601 ggaagccgag cgttttgttt tggctgggcg cgctgttcct gtggggcagc cacctcgccg
    15661 gtaaaaacgg cttgaaggcg agtatcggca gggagattca gctttcagat gccgtatggg
    15721 cgaaattgac gtatatgtgg gtcggttttc tgatttttat gggtattgcc aactggtttg
    15781 tgtttactag gtttgaagcg caatgggtca actataaaat gttcggctcg actgcactga
    15841 tgcttgtttt ctttattatt cagggtattt atctgagtac ctgtctgaaa aaggaggatt
    15901 gactgtggaa tattttatgt tgctggcaac agacggggag aatgtgtatg aagcgcgtat
    15961 ggcggcacgt cccgaacacc tcaaacggct ggagacgctg aagtcggaag gccggctgtt
    16021 gacggcaggc ccgaatcctt tgccggagga ctccaaccgc gtttcgggca gtttgattgt
    16081 ggcgcagttc gagtctttgg atgcggcaga ggcgtgggcg gaagacgatc cctatgttca
    16141 tgcaggcgtg tacagcgaag tgctgatcaa gccgtttaaa gcggtgttca aataatgccg
    16201 gccgtcgatt tgatccgcga acgcctgcag acgctcgatc cgctggtgtt ggaaatcggc
    16261 gatgagagcc atctgcacaa aggacacgcg ggcaataccg gcggcggaca ttatgccgtt
    16321 ttggtcgtca gcggccgttt tgaaggcgta agccgcctga accgccagaa aacggtcaaa
    16381 tcgctgctca aagatttgtt ttcaggcggc atgattcacg cgctcggcat ccgggcggct
    16441 acccctgacg agtatttcca tacggcggac tgaatgaagt ctgcccgaac atttcaattt
    16501 aaaatttaaa gagagaagat tatgaaagca aaaatcctga cttccgttgc actgcttgcc
    16561 tgttccggca gcctgtttgc ccaaacgctg gcaaccgtca acggtcagaa aatcgacagt
    16621 tccgtcatcg atgcgcaggt tgccgcattc cgtgcggaaa acagccgtgc cgaagacacg
    16681 ccgcaactgc gccaatccct gctggaaaac gaagtggtca acaccgtggt cgcacaggaa
    16741 gtgaaacgtc tgaaactcga ccggtcggca gagtttaaaa atgcgcttgc caaattgcgt
    16801 gccgaagcga aaaagtcggg cgacgacaag aaaccgtcct tcaaaaccgt ttggcaggcg
    16861 gtaaaatatg gcttgaacgg cgaggcatac gcgctgcata tcgccaaaac ccaaccggtt
    16921 tccgagcagg aagtaaaagc cgcatatgac aatatcagcg gtttttacaa aggtacgcag
    16981 gaagtccagt tgggcgaaat cctgaccgac aaggaagaaa atgcaaaaaa agcggttgcc
    17041 gacttgaagg cgaaaaaagg tttcgatgcc gtcttgaaac aatattccct caacgaccgt
    17101 accaaacaga ccggcgcgcc ggtcggatat gtgccgctga aagatttgga acagggtgtt
    17161 ccgccgcttt atcaggcaat taaggacttg aaaaaaggcg aatttacggc aacgccgctg
    17221 aaaaacggcg atttctacgg cgtttattat gtcaacgaca gccgcgaggt aaaagtgcct
    17281 tcttttgatg aaatgaaagg acagattgcc ggcaaccttc aggcggaacg gattgaccgt
    17341 gccgtcggtg cactgttggg caaggcaaac atcaaacctg caaaataatt ctgaaaacgg
    17401 gatatggcgg caagacgttc agacaggcgt tttgccgccg cgcaggacag ggaataccat
    17461 gaaacagaaa aaaaccgctg ccgcagttat tgctgcaatg ttggcaggtt ttgcggcagc
    17521 caaagcaccc gaaatcgacc cggctttggt ggatacgctg gtggcgcaga tcatgcagca
    17581 ggcagaccgg catgcggagc agtcccaaaa accggacggg caggcaatcc gaaacgatgc
    17641 cgtccgtcgg ctgcaaactt tggaagtttt gaaaaacagg gcattgaagg aaggtttgga
    17701 taaggataag gatgtccaaa accgctttaa aatcgccgaa gcgtcttttt atgccgagga
    17761 gtacgtccgt tttctggaac gttcggaaac ggtttccgaa agcgcactgc gtcagtttta
    17821 tgagcggcaa atccgcatga tcaaattgca gcaggtcagc ttcgcaaccg aagaggaggc
    17881 gcgtcaggcg cagcagctcc tgctcaaagg gctgtctttt gaagggctga tgaagcgtta
    17941 tccgaacgac gagcaggctt ttgacggttt cattatggcg cagcagcttc ccgagccgct
    18001 ggcttcgcag tttgccgcga tgaatcgggg cgacgttacc cgcgatccgg tcaaattggg
    18061 cgaacgctat tatctgttca aactcagcga ggtcgggaaa aaccccgaca cgcagccttt
    18121 cgagttggtc agaaaccagt tggaacaagg tttgagacag gaaaaagccc gcttgaaaat
    18181 cgatgccatt ttggaagaaa acggtgtcaa accgtaatgg catttccaat accgatgccg
    18241 tctgaagcct tttcagacgg cattgcacgt tcaggtaagg aggacggctt atgcgtgcgg
    18301 tcatacagaa aacggtaggt gcaaaggtgg atgtcgtgtc cgaaaacggc acggaaacct
    18361 gtggcaaaat cgacggcgga ttcgtcgtgc tgctcggcgt aacgcatagt gacacagaaa
    18421 aagatgcgcg ctatatcgcc gacaaaatcg cccatttgcg cgtgtttgaa gacgaagcgg
    18481 gcaagctgaa cctgtctttg aaagatgtcg gcggcgcggt gctgctggtg tcgcagttta
    18541 cgctttatgc cgacgcgaca agcgggcggc ggccttcgtt ttcgcaagcc gcacctgcag
    18601 aacaggcgca gcagctttac ctgcgaacgg cggaactgtt gcgcggacac ggaattcatg
    18661 tcgaaacagg gcgtttccgc acgcatatgc aggtgtcgct ctgcaacgac gggccggtaa
    18721 ccatactgct ggactctttc atgacgcgga tttccccaaa aatgaaggtt gttccggatt
    18781 gaaattgaat ccgcaatggt gaaatatcga caatgaacga caacacgcaa acccttcctc
    18841 cccgccgcct gtccgtcgcc ccgatgttgg actggacgga caggcactac cgttatatgg
    18901 cgcgccagat tacccgcaat acttggctgt acagcgaaat ggtcaacgcc ggcgcgattg
    18961 tttacggcga caaagaccgc tttttgatgt ttaacgaagg cgagcagccc gtcgccctgc
    19021 aactgggcgg cagcgacccg tccgatttgg cgaaagccgc caaagccgcc gaagaatacg
    19081 gctacaacga ggtcaacctc aactgcggct gccccagtcc gcgcgtccag aaaggcgcgt
    19141 tcggcgcgtg tctgatgaac gaagtcgggc tggttgccga ctgcctcaac gccatgcagg
    19201 atgcggtcaa gattcccgtt accgtcaaac accgcatcgg tgtggacagg cagaccgaat
    19261 accaaaccgt tgccgatttc gtcggcacgc tgcgcgacaa aaccgcctgc aaaaccttta
    19321 tcgtccacgc acgtaacgct tggctggacg gtctttcacc caaagaaaac cgcgacgttc
    19381 cgcccttgaa atacgattat gtttaccgcc tcaagcaaga gtttcccggg ctggaaatca
    19441 tcatcaacgg cggcatcacg accaatgagg aaatcgcgca acacctgcaa cacgttgacg
    19501 gcgtgatggt cgggcgcgag gcgtaccaca acccgatggt gatgcgcgaa tgggacaggc
    19561 tgttttacgg cgatacccgc agcccgattg aatacgccga tttggtgcag cgtctctaca
    19621 catacagtca agcccaaatc caagccggac gcggcacaat cttgcgccac atcgtccgcc
    19681 acagcctcgg gctgatgcac ggtctgaaaa atgcccgcac ttggcggcgt atgctttccg
    19741 atgcaacgct cttgaaagac aacgacggcg gcctgattct cgaagcgtgg aaagaagtcg
    19801 aacgggcaaa tatgcgcgaa tagggcgggg ctgtgtgtat gaaatgccgt ctgaaggttt
    19861 cagacggcat ttgtgcgttt gtcgggcggt gtttaggggg cggtaatggc gtgtttcggc
    19921 actttgccca tatcccagtg tgccaccgcc cagtcgagca gttcggcagg gcggtcggtt
    19981 tccggtgctt cgggcagctt gaggtaacgg aacacttggc ggaggagttg ttcgcggcgg
    20041 tttaaatcca atgcgggggc gagcgtctgt ttcgaccact tctgcccttg tgcgttggtc
    20101 agcagcggca ggtgggcata ttgcggtgtc ggaacgccca aacactgctg caaatagatt
    20161 tggcgcggcg tggaaacgag caggtcttgt ccgcggacga tgtgggtaac gccctgttcg
    20221 gcatcgtcgg caacgacggc gagttggtat gcccagtaac cgtctgcacg aagcaggacg
    20281 aaatcgccga tgtcgccggc gaggttttgg gcgtaaccgc cgacgatgcc gtctgaaaaa
    20341 ccgataatgc ggtcggggac gcggatgcgc cacgccggct gtttgccttg cggtgcaggg
    20401 cgttgctgcg gattgcggca gcgtccgtta tagacgaacc cgtctgcgcc ccgccttgcc
    20461 ccggtctgcc agtctttgcg gctgcaatgg cagggataga ccagtccggc ggttttcagg
    20521 cggcatagtg tttcttcata cagggcgtaa cggcggctct gataggcgac ttctccgtcc
    20581 cactcgaatc cgaatgcctc aagcgtgtgc aggatatggc ttgccgcccc cggcatttcg
    20641 cgcggcggat cgaggtcttc catgcggacc agccatttgc cgccgtttga gcgtgcatcg
    20701 gcataggaag cgacggcggt cagcagcgag ccgatgtgga gcagcccggt cgggctgggg
    20761 gcaaaacgtc ctgtgtacat atctggtaca gcccctttat ttaagactat ggcggattcg
    20821 catttgaagt gcaactttcc ctaacagaaa aaggccagta tgcggtagca tacg
//
LOCUS       DAXHKX010000017        20690 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHKX010000017 DAXHKX010000000
VERSION     DAXHKX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.017"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(64..240)
                     /locus_tag="ACFMWZ_000550"
     CDS             complement(64..240)
                     /locus_tag="ACFMWZ_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934551.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(243..1601)
                     /gene="murF"
                     /locus_tag="ACFMWZ_000551"
     CDS             complement(243..1601)
                     /gene="murF"
                     /locus_tag="ACFMWZ_000551"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC5934552.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQTLAKVWRENVNPF
                     VFGITGSVGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1725..3371)
                     /locus_tag="ACFMWZ_000552"
     CDS             complement(1725..3371)
                     /locus_tag="ACFMWZ_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC5934553.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3394..4872)
                     /locus_tag="ACFMWZ_000553"
     CDS             complement(3394..4872)
                     /locus_tag="ACFMWZ_000553"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC5934554.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDGYGAELIGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACIALLCANDYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRLLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4897..6642)
                     /locus_tag="ACFMWZ_000554"
     CDS             complement(4897..6642)
                     /locus_tag="ACFMWZ_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC5934555.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLVYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="ACFMWZ_000555"
     CDS             complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="ACFMWZ_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC5934556.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="ACFMWZ_000556"
     CDS             complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="ACFMWZ_000556"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC5934557.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQAALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="ACFMWZ_000557"
     CDS             complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="ACFMWZ_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC5934558.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8707..9897
                     /locus_tag="ACFMWZ_000558"
     CDS             8707..9897
                     /locus_tag="ACFMWZ_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC5934559.1"
                     /translation="MSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLIAEEIGKHGN
                     WIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQLQELLSQT
                     AGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAPEASQKVV
                     HLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSARPLHDLQ
                     LSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQYYHPQRN
                     QGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQSHFLLNL
                     GITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVDWAGFRFGDI
                     CHKL"
     gene            10029..10105
                     /locus_tag="ACFMWZ_000559"
     tRNA            10029..10105
                     /locus_tag="ACFMWZ_000559"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10063..10065,aa:Met,seq:cat)
     gene            10113..10188
                     /locus_tag="ACFMWZ_000560"
     tRNA            10113..10188
                     /locus_tag="ACFMWZ_000560"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10146..10148,aa:Ala,seq:ggc)
     gene            complement(10470..11291)
                     /locus_tag="ACFMWZ_000561"
     CDS             complement(10470..11291)
                     /locus_tag="ACFMWZ_000561"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC5934560.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEITIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIVDVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11324..11968)
                     /locus_tag="ACFMWZ_000562"
     CDS             complement(11324..11968)
                     /locus_tag="ACFMWZ_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC5934561.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQKQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVKLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAEKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11971..12816)
                     /gene="ftsN"
                     /locus_tag="ACFMWZ_000563"
     CDS             complement(11971..12816)
                     /gene="ftsN"
                     /locus_tag="ACFMWZ_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC5934562.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREKSDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12922..14418)
                     /locus_tag="ACFMWZ_000564"
     CDS             complement(12922..14418)
                     /locus_tag="ACFMWZ_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC5934563.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14432..14752)
                     /locus_tag="ACFMWZ_000565"
     CDS             complement(14432..14752)
                     /locus_tag="ACFMWZ_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC5934564.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15235..16761
                     /gene="putP"
                     /locus_tag="ACFMWZ_000566"
     CDS             15235..16761
                     /gene="putP"
                     /locus_tag="ACFMWZ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC5934565.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     NVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SNPDSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIIAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            complement(16851..17018)
                     /locus_tag="ACFMWZ_000567"
     CDS             complement(16851..17018)
                     /locus_tag="ACFMWZ_000567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934566.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17010..20615
                     /gene="putA"
                     /locus_tag="ACFMWZ_000568"
     CDS             17010..20615
                     /gene="putA"
                     /locus_tag="ACFMWZ_000568"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC5934567.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKGRLNSQGVDFSNETVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRVLPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4839 a         5979 c         5049 g         4823 t
ORIGIN      
        1 tcgggcctgc gcccgtttga ggttggcttg ccgcccggca aggtttcgga cggcttttgc
       61 agattaacgt gcagggactt tctcaatcac gcaaattggg taactccccg gatttttacc
      121 gccgcccaag tctaaacata aatcttcata aagatacgcc tgcgtccgca ttccggcata
      181 aaacgcgccg accagcgcgg caatcagcaa taaggcttta aaaaaacgtc ggcttttcat
      241 tttcacttat cctccaatgc ctcgaccact tcttccatct gcataaagcg cgaacctttc
      301 accaacacgg tggcgcgttc gggcaaatcg tggcgcaaca cttgaatcaa cgggtctttg
      361 gcggcgaacc acaaaccgtc cgcgccaaat ttttccgccg cttcgacgct gttgtcgccg
      421 acaaaataag ccgcttcgat gccttggtcg cgggcatacg cgccgacttc ggcgtgcata
      481 gcggcggctt cgtcctcgcc cagttcgccc atatcgccca ttacgaaaat acgcggcgca
      541 ggcatacgcg ccaacacgtc aatcgcggct ttcatgctgt ccgggttcgc attataagta
      601 tcgtcaatca gggttgcgcc cttgattccg gatttgacgt tcagacggcc tttgatattg
      661 ctgaagcctt tcaaaccttc cgccacatcg ttcaaactca aacctgcagc caaagacagc
      721 gcggcggcag cggcggcgtt gtggacattg tggcggccgg gaacaggcag caccacggcg
      781 gcgcgttcgt cgccgcacac caaatcaaat tcgcacgaca acggtttcag cacgatattt
      841 tccgcgtgga catcgccgct atcgatgccg aaagtgcgcg tattcaaatt aagcgttgcc
      901 gttttgaaga cagccatatt ggcatcttct tgaggaatca gtgccatgcc gtctgaacat
      961 aagccttgat aaatctcgct tttcgctttg gcaatatcgc ccactccgtc gaaaccgcag
     1021 ccgacatggg cgcgcatggc gttgttgacc aatgcggcat cgggtttggc gatttgtgtc
     1081 aaaaccgcca gttcgccaaa atggttcata cccatttcaa tcacggcata gcggtgtttt
     1141 tcgtttaatt tcaacaaagt caacggcaat ccgatgtggt tgttgaagtt gcctgccgtc
     1201 gccaaaacgg cattatcgcc gaaacggcgg cgcaataccg cagccagcat ctccttcacc
     1261 gtcgtcttgc cgaccgaacc ggtaatgccg aacacaaacg gattcacatt ttcacgccac
     1321 acctttgcca acgtttgcaa tgcggcaagc gtgtcatcga ctttcaacgc gccatccatt
     1381 gcaacgcaat cttcgcgcga aaccacaacc gccgccgcac cagcagccaa tacgtcttca
     1441 acaaaatcat gcgcatcaaa ccgcccgccc gccaatgcga aaaacacatc gcccgcgcgg
     1501 atgtcgcggc tgtcggttac gatgcgcgac acgggtttgc tttcagacgg catcggaagc
     1561 ttgagggctt ggcagatgaa atttaggtcc agtggtttca tatttacttt cgttaatatt
     1621 cgggcggcgg acacatcggt agcggctgat tttttatcgc ctgttttact gtggtaaaac
     1681 acagattatt ttcccattct cattcggcat tttttctgta cgtatcattt tttagacgta
     1741 tttttagtcg atttgccttt tcccgcatac cacggcgcgg ggtcgtcggg cagtccgtcg
     1801 ataaaggcaa ggttattgcc ttcgccctgc acatcgggaa cattccccca aaaatcatag
     1861 ccgtcatcgg gcaactcgtc ggtttcgata cccgtccaac tgccgaatcc gcgtaaaaaa
     1921 ttaaacgcgc tgcgcctcac tttaatcatt tcgcgccgcg tgtcatcgga cgaaatttta
     1981 accagcggca cgccgtagct ttgacgcttc cacgcaccat gtgtcaacgt tcgctcgccg
     2041 cctttaccga catgcatcaa accgtggtcg gaaaagtaaa ccaaagacca gctttcttta
     2101 ttttcattca atatcttaac tgtatcttct aaaaatttat cggtttgcgc gatggtggaa
     2161 acatagcagg atattttttc agtttgatac tgaaaccgcc gcgcatcctt atccaaacgt
     2221 gtgcaaaaat cactgtgcga acccatcagg tgcatcacaa tcagccgagg cttcgtgcct
     2281 gcatttccta tcaaaacccg tttgaacgcc ggcaacaaaa ggcggtcgct caaccccgcg
     2341 cttttgccat aatcacccct ttgggtaaac cacggataat cgctgcgtag ggcataggtg
     2401 gaaatttcgt tggcaaaatg ccccaacatt ccttgattag acagccacgc cgtccgaaaa
     2461 cccgcctgct tcgccaagct gacgatgtta ttgttcggtt ctcccggcag ccccaaagtc
     2521 tgcggcagcg aaagattcgt cgcgtgggcg gtcgattggt aaccgtttat caacagccct
     2581 ttggtctgac tcaaaaaagg cgtatcgggc aatgggaaac cgtaaacatt catataatcc
     2641 gaacgcgcgc tctcaccgat aaccacaaca taatttttat acttgggcgc aacatgacgg
     2701 atatgccatg ttgacgcttt ttttgctgct tccaaaatgt gggcgcgctt ggcggcatat
     2761 tgtgccggcg cggaagccaa atcgtaatac aggtcgaaaa tattcaacaa caggccggca
     2821 tcaggttctc gcaaatcttt atcgctggcg attttatcca tcaccgcgca ggacaaaatc
     2881 aaagtcaata atatagtcag ccatattttg ctgcggcgtt tatagttttt tacgtcagca
     2941 aatacccccc ccgatacaca atatctccaa actgtcaagc caaaaatgaa aaataatgcc
     3001 tgcacaaaat aaagcgaccc gggaagattg ccgacaaatt cacgcgcctc ggcaggattg
     3061 ctttccaata tcgaaccgac tatctgataa gacggcgcac catacagcca gccgaccggc
     3121 aaatataggg cggttgtgcc gacgtaaatc agtaaaacaa ctgaagaaac gcgcgggaaa
     3181 ccgcgtgcca ataataataa aaatacaacc gaagccaaag cacccacggc agaataacgg
     3241 tagccgtatt catattccaa gtgataacct gatgcgatgg ccgcgccaaa acaaaaggct
     3301 aaaagcgcgc aaatgcccaa tgtatttttt aatgtctgat ccatattttt tattcgggcg
     3361 gattgtttca tacgatgccg tccgaatatc ccattattta cgagttaaca aagcctgtcc
     3421 gacgatttca agatcggaaa aacggtgctt cacgccttgt acatcctgat agttttcatg
     3481 ccctttgccg gcaatcagga tgatgtcgtt tgcggcggct tgttcaaccg cataacggat
     3541 ggcggcggca cggtcggctt cgacgcattc gggcgcggga acggcaggca ggatgtcgtt
     3601 gatgatgtcg tgcggatttt ccaaacgcgg attgtcgctg gtgacgacga ctttatccgc
     3661 gccctgtacg gctgccgcgc ccatcagcag gcgtttgccg cgatcgcggt taccgccgca
     3721 accgaatacg caccacaaag ccgcaccctg cggtttgatt tcctgcaagg tggcgagtgc
     3781 tttttccaat gcgtcgggcg tgtgggcgta atcgacgacg accaagggct tgccgctgtt
     3841 catgatgcag tccatgcgcc ctgaagcggg acggattttt gccagcacat ccaatacctt
     3901 atccaacgga tagtcgttgg cgcacagcaa ggcgatgcag gcggcgaggt tttgcgcgtt
     3961 gaaccgtccg agcaggcgtg tgcggcattt cccttcaccc cacggggttc ggaatacggc
     4021 ttctatgccg tctgaagagg cggtaaagtc ggtaatgcgg atgtcggcgt gttcgctgaa
     4081 gccgtagcta taaacggcca aatcggggca gtcttttttc agacggccta tgagttctgc
     4141 gccgtatccg tcatctacat tgataacggc gtgtttcaag ccatgccagt aaaacaggcg
     4201 cgacttgatg gcgccatagg cttccatcgt gccgtggtag tcgaggtggt ctcgggtgag
     4261 attggtaaag attgcgctgc ggaatggcac gccgttgacg cgcgactggt caagaccgtg
     4321 gctggagact tccatcgcgg cggctgttgc gccttgttga cggaaacggt agagcagggt
     4381 ttggacatcg acgggggcgg gtgtggtatg cgtggtttct tccaatgcac cccaaaagcc
     4441 gttgccgacc gtgccgacaa tggcggtttt ttcgcccaac aaatcggcag cttgcgccag
     4501 ccattgcgtg atggaggttt tgccgttggt tccggttacg ccccagaatt tgagaccgtc
     4561 tgaaacgttg ccgtaaactt gcgccgccaa tatgccggca cggtgtttca aatctttgat
     4621 gccttgattg gggactttcc attcgggatt ccacgcgaat ttgccgtcgt cgtcccaaaa
     4681 aacaaaagcc gcgccgttgg caacggcggc ggggatataa ctgcggccgt cggcatattc
     4741 gcccggacag gcaacgaaaa tatcgccttg tttgatttgg cggctgtctg aatgcaacaa
     4801 acgccctgct gcgtttgcac acgacagggt cgggatgccg gtttcagcca aaggggttaa
     4861 cttgctgaac ataaaacaat ctcgttgata ctcggattaa gacggtgttt tgacggctgc
     4921 ggcggtcagt ggcttggtcg gggaaatgcc caagatgttc aggctgccgc ccataatttt
     4981 tttgaagggc ggccctgcca ctacgccgcc gtaataaccg tgggcagtcg gttcgtcaat
     5041 ggttaccgcc acaatcacac gggggttttt ggcgggggca aaaccgataa aggtagcgat
     5101 gtgtttgttg tcggcataac gcccgttgac gaacttgcgc gccgtgccgg ttttcgcgcc
     5161 gacatcgaaa ccgtccaccg cacccgccgt accggtgccg cccggctcgg tcacggaaac
     5221 catcagattg cgtacctcgc gcgcggtcga ttctttgaat atgcgtttgc cttgcggcgc
     5281 aaccgcctgt ttttcaaagc tgaccggcag caaaacgccg tcgtgcgtca gtgcggtata
     5341 ggcgcgcgcc aattgcagca ggctcaattg caggccgtaa ccgaaagaca tcgtcgcctg
     5401 ttcgataggc cgccacctgc gccaatttct caacaaacct gcagtttcgc ccggaaagcc
     5461 cgagtgcata cgcacaccga tgcccaactc atgatagaag tcatacattt cttcggcgcc
     5521 gaaacgcgca gacagtttgc ttgtgccgac gttggacgat ttctgcatga tgccgcgcac
     5581 atccaaagag gggtaaacat gggtatcgcg cacgggagac ggtccgattt tataaggctg
     5641 cgtattcagc cgttcgttca aatcggtttt gcccgcatcc aatgccttcg caatcacaaa
     5701 cggtttgatt gccgaaccgg gttcgatcat atcggttacg gcacggttgc gccgctgttc
     5761 gctgtctgcc cggccgggcc tgttgggatc gtaggcgggc gtattggcca aggcgaggat
     5821 ttcccccgtg cgggcatcca aaaccaccac cgttccggct tttgcctgat ggtattcgac
     5881 cgccttgttc aactcttcat agaccaaggt ctgaatcctc tgatcgaggg aaaggatgat
     5941 gtctttgccg tttttcgggg ctttattgcg cggggagtcc aagctgtcca caatattgcc
     6001 ctgccggtcc cgcaaaacga cttccgcgcc gtcttcgcca tgcaggctgt cttcaagcga
     6061 aagttccaaa ccttcctgac ctttgccgtc aatatcggta aatccgatga cgtgtgcaaa
     6121 caggttgccc atcgggtaat ggcgttttaa ttctttttca aatacaaagt tttccaaacc
     6181 caaggctttg acctcttcgg caaccttggg atcgagctgc cgcttaatcc agataaacga
     6241 cttgcctttc tgttcgagct tgttcctcaa aacatcaacc ggcacatcga caagctcgga
     6301 caggcgttcc aattgtgcgg cagacggcat ttccttcatc tctttaggca cggcaaacag
     6361 ggactccgtc ggcgcactca acgccaaaac cgcaccgttc cggtccgaaa ccgtaccgcg
     6421 tgtagccggc aatgtttgag tccgcacaat ccggttgtcg ccctgttctt tcaaaaagtt
     6481 atacgttacc gtctgcagat acagtccgcg ggcaatcaga ccggcaaaca agaccgctat
     6541 tgccatcagg acgaagctga tccgtccgtt actggtcatc ggctttttga cctgctcttc
     6601 tttgggcagc atccgaggct tatattcgct cttaatcaac atttttactt ctcgttatta
     6661 ttatcctgac gcaggaatcc gattccggca cacaggctgc ttctatcttt gatgctccac
     6721 cataaaggta ttgcccgaaa ccggcggatg gaggttttgt ttttctgccg ctgccctgat
     6781 cgcttcgtgg ttcgccaaac gcgcctgttg cagcctcatt tgtgcataat cctgctccaa
     6841 ggcgatttcc tgttttttcg ccttgtccaa agctgtgaag ttgagcctgt actggttttg
     6901 ctgcatcaca acggaaaaag cggaaacgca caccgcaagc agcagaagga aattcaattt
     6961 gttcattgcc attcagacgt ttttctctgt gattgttccg gtatcggacc ggcagtccgc
     7021 tccgccacac gcaaaaccgc acttctcgcc ctcggattgg cggcaatttc cgcctcaccc
     7081 ggctttaatg ccctgcccac gattttcagg ggcggctcgg gcaaatccgc ttccctgacc
     7141 gccgcccaac gcggcagggg cgcgtgttgc gaatattttt tgacaaactg cttcacaatg
     7201 cggtcttcca acgaatggaa agcaatgacc gccaaacgtc cgccctcttt cagacgacac
     7261 atgacctgcg gcaatactgc ccctacttct tcaagctcgc ggttaataaa gatgcggacc
     7321 gcctggaagg tgcgcgtcgc aggatcctgc ccccgctcgc gagtacggac gttttgtgcc
     7381 acgatctgcg ccagcttgcg ggttgtatcg attggacttt ccgcccgttg cgcaacaatg
     7441 gcgcgcgcaa tctggcggct aaaccgctct tcaccataat tcttgattac ctcgtgcaaa
     7501 tcctgttccg acgcaaccgc tatccactct gcggcagaca taccgcgcgt cgtatccata
     7561 cgcatatcca aaggggcatc gaaacggaag ctgaaaccgc ggctgccgtc atcgatttgc
     7621 ggagacgaaa tccccaaatc aaacagcgca ccgtccacct tgccgatacc caaaccgtcc
     7681 aatgccgcct gaaacgaagc aaaaccgcca tgcacgacac cgacccgttt gtccgaacgc
     7741 gccagctctt ctgccacagc aatcgcctga gggtctttgt cgaaaacaat caaccgcccc
     7801 gcatcgccca aacgcgacaa aatcagccgg gaatgccctc ccctgccgaa cgtaccgtcc
     7861 acatagacac cgtcttcgcg cacggcaagc gcatccaccg cctcattcag caagaccgtg
     7921 atatgccggt aactttctgc tccactcaca attgcaaatc cgtctgaccc aactggaagg
     7981 caagttcgtc aggatcgtca tccaaagcct gaaccatctc agcctcccac tgctcgcgac
     8041 cccaaagctc caaacggttg gcacgaccga ccaaaacgac ttcacggtcg aaatccaccc
     8101 ttttcctcag tccggcagga agcagcaccc ggccggcgct gtcccattcc aaaatttccg
     8161 cgttatgcag caaaagattt tgaaaccgcc gcaaaacagg gttatccgcc acttttaagt
     8221 ttaaaagttg cgccgcaacc ttttcccact ccgcaacagg gtacatcaac agcttgtgtt
     8281 tcgactcgag cgttaccact acggcaggcg tatagaggcg cgacagaatg tcacggaatt
     8341 tggcaggaac agccaaccgc cccttactgt cgatgcttaa ttcgtgtgcg ccgccgaaca
     8401 tgacatgtcc caagccgaaa tcaaaatcac aagggtaaaa gagacacttt gccccacaat
     8461 tccccaccaa tcgacactat aagaaatttt aaacactcgg tcaaatcagg gcatgaaaac
     8521 tcattaacat atctgaaatt tttattcctt ttaaaaacaa taagataaaa aatgacgaca
     8581 acggcacggc gggtgcggta cagaataatc gaaccaataa acaactatat atgattaatt
     8641 taataatata aacacaatat atagtattaa gataaagcca tgacagcacc cgtaccaacg
     8701 tgtaatatgt cgggaaatcc aataaattta cacaagctaa cacttatcat gcccctcccc
     8761 ctcccctctc ccgaagcacg gcaattctcg ctcaaactgc aaaccctcat tgccgaagaa
     8821 atcggcaaac acggcaactg gattccattt tcacgtttta tggaattggt tttatacgct
     8881 ccgcaatacg gctactacac cggcggcagc cataaaatcg gcaataccgg ggattttatt
     8941 accgcaccga ccctcacctc tctgtttgca cagacactgg cacgccaact tcaagaactt
     9001 ctatcccaaa cggcgggcaa tatctatgaa ttcggcgcgg gaaccggaca gcttgccgcc
     9061 gatttgttgg gcagcatttc ggacggcatc agccgttact atattattga aatatcgccg
     9121 gagctggcag cacgtcagaa aaacctgatt caagcacgcg caccggaagc atctcaaaaa
     9181 gttgtccact tgaccgcact tcccgaagcg tttgacggca tcatcatcgg caacgaagta
     9241 ctcgatgcca tgcctgtcga aatcgtccgt aaaaatgaag gcggctcatt cgagcatgtc
     9301 ggcgtttgcc tagataatga ccgttttacc tattcggcac gaccgctgca cgacttacag
     9361 ctatctgcct tggcttccct ctattttcct caaacagatt atccctacac cagcgaacta
     9421 catccgcaac aatatgcctt tatccgcacc cttgcctcaa ggcttgaacg cgggggtatg
     9481 atattcatcg actacggttt tgatgcagcg cagtattacc accctcaacg caatcaaggt
     9541 actctgatcg gacactaccg acatcacgtt atccacaatc cttttgactt catcgggttg
     9601 gccgacctga ccgcgcatgt caactttacc gacattgccc aagccggtac ggatgcagga
     9661 ctggatttga caggctatct gccccagtcc cattttctgc tgaatctggg cataaccgaa
     9721 ctgttggcac aagtgggaaa aacagactcg cccgcctaca tccgtgaagc tgctgccgtt
     9781 cagaaactga ttgaccagca tgaaatggga gaattgttta aagtcatcgc attcggtaaa
     9841 aatatcggcg tcgactgggc aggattccgc ttcggcgaca tctgccacaa actctaaccc
     9901 tcatgccgcc tgaatccgct tcagacgaca taaacttttt aacatttaaa aacagtcaac
     9961 taattcaaaa ttaaaaaata cggcttgtca aaaaaacaga aaaacatata atagcgtctt
    10021 cacgaaacgg cgaattagct cagtcggtta gagcagagga atcataatcc ttgtgtccgg
    10081 ggttcgagtc cctgattcgc caccaaattt tcgggggtat agctcagttg gtagagcgct
    10141 tgcatggcat gcaagaggtc agcggttcga tcccgcttac ctccaccaga taaaaaagca
    10201 cagaccgtaa aaaggtatgt gcttttttat tgcctgattg ccagcaaata aagaataaac
    10261 cactgccttc aaaacaggca atcgacttta aacctatcgc cccgcctgtc ctgattttat
    10321 agtgaattaa atttaaaccg gtacagcgtt ggctcgcctt gccgtactat ctgcggcttc
    10381 gtcgccttgt cctgattttt gttaattcac tatatcagcc cgccaaacaa acccgacccg
    10441 aataatgtct tcaggtcggg tttatggttt cattcccaac ttatccagcc tgacagccac
    10501 aatataatga tggcaatgcc aaaaacaatg cggtaatagg caaaaggaat ataatttttc
    10561 ttggaaacaa acctcagcaa cgcttttacc gccaccaaac cggaaacaaa agcggcaaca
    10621 aagccaatca gaatcaaacc gacatcatgc agggtgaaaa accggtaatg cttcaggaca
    10681 tcataagccg ttgctgcaac catcatcgga acggccaaga aaaatgaaaa ctccgttgcc
    10741 gttttccgct cgattcccca aagcatcccg cccataatcg tactgcccga acgggacgta
    10801 cccggaacca gtgcaaacac ttgggcaacg ccgatcatca acgcatcaat cggacgcaat
    10861 gcatcaacat cgacaatttt aggctctgcc cggctttggc gtttctccac ccacaaaata
    10921 aaaaaaccgc ccaaaaccag catgactgca acactcaagg ggttaaacag atactctttg
    10981 atttgtttgc cgaacaacag ccccatcacg gcggcaggta taaaagcaat ggcaagatta
    11041 aggacgaaac ggttggcttt ccggtctttt cccacgccat gcaacacatt gctgaaacgc
    11101 tgccggtatt caaacactac cgccaaaacc gcaccgagct ggatggtaat ttcaaaaacc
    11161 ttgtgattgc tgtgaaaatc aatcagattg ccgaacacaa tcaaatgtcc ggtgctggaa
    11221 atcggtaaaa attcggtaaa accttctacc aagcccatca tcagggcttt caggacaatc
    11281 agaaaatcca ttgcttgcgc ttctttcgga tacgggagtt cggctatttc tgtacagcag
    11341 gggtctgacg cttgcgttct tccctgactt tggcaaccaa ttctttaatc gtatgaacac
    11401 cgccgtcaaa gccattattg aagataacgc ggtattttcc gccgacaata acggtcggcg
    11461 tgctgtcaat accgtattgt tccgtcagtt tctgcatttt taatgcggcg gcggcagctt
    11521 cgggagaatc ataggcgcgc atcagttttt tgccgtcaaa gcctttttga gacaaagccc
    11581 atttttcggc aaccgacctg ttttccaagc ggattttttg ttcgtaaact gctttaaaca
    11641 cggcagggtt tgcctgatat ttcaaacctg acagcttgac cgcagcagcc attcttgcca
    11701 gaccgagcat ttcaggctgc cagaccacgt gctccgtcct taaataggca tcagacggca
    11761 atgccttgcc caatttcaat aacaaaggat cgaaatgatg gcaatgtacg cagaaatagc
    11821 cgaaaaattc caaaacctca attttgcccg actgtttttg aggaatgggt ttatccaaca
    11881 caagatagtc ttccccttcc gtcagggcat atgcctgcgc ggacaacact gccgacagca
    11941 gcagcggcaa cagatgtttg agcttcataa ttatttactt tcgatagaac ggatcaggct
    12001 ggcgacttca tgttttttca actcgtcctg catttttttc accgcatcgg cagacatatt
    12061 gccgctttgc acccggtaaa gcgttttatg tcccgcctga tagccgacca ccttggaaga
    12121 tatgcccaag attgccagtt tggcacgctg cccttccgcg ctccggcggt cggcatacgc
    12181 gcccatttgc agataatgcg ttgcttccgc cttgtcggac gttttcattt tctgcacttc
    12241 tttggcggcg gcactgcgcg ctttttcgat gctgccgctg ttgaggattt gttccggggt
    12301 cggtttgggt gcaacttttt ccttttccgc ctttttctct tcttttggag cttttttctc
    12361 tgtttcttta gacggtttta ccgcttgttt tttaaccgtt tcggcatctt tcttctgcgc
    12421 tttttctctg acggtttgtt cacgctcttc cgtcagcact ttcttacgca ctgcctgtcc
    12481 gtccgacttt tcccgctccg gctcgcccgc cttttcttca acctcgtcgg ctttatcggc
    12541 agctttttcc gcatccgact gctctgcctc tgtcgcagca tccggttcgg acaaggcgtt
    12601 ttgatcggcc ggttcaggtt ggatgtcttc cttaggctgg tttttcggtt tcaggatttc
    12661 cgtttctgca ggctgcttcg acgaagccgg gattttgaac gcattttgac cgctctggtt
    12721 cagataaaac aaaataccgg caataatgac cgtcgccagt atcaaaccga agaaaaaacc
    12781 ggacagacct tttccggatt gggaaaattt gttcataaac ataccttaat gtgtttcaga
    12841 cggcattaac gccgtttgct tacgggcgga tattctaaca atatcgccat atttgggcaa
    12901 aacctgcttc cattcccatt cctataaagc acgacggaaa cctatggctt tcatgacgtg
    12961 gcttctgccg acttcttcgt cgccagccaa atccgccaat gtacgcgcca cgcgcatgat
    13021 gcggtggaag ctgcgggcgg aaagggagag tttttccagc aggccgccca atgcttcctg
    13081 cgcttctttt tggatgcagg cttgggaatc gagttcgctg acgctcaaag cggcgttgac
    13141 tttgccctgc cgtgcgtatt gtttgtcgcg ggcggcgata acgcgttcca aaacggacgc
    13201 gctgctttcg cccgcttcct gctgcatcag ttcggcggcg gacaggctgg ggacttcgat
    13261 ggtcaaatcg atgcggtcga gcagcggccc ggaaatcttg ctgcggtaac gcgcgacgct
    13321 ttcgggcgtg cagcggcagg gtttgacggg atgcccgaga taaccgcacg ggcaggggtt
    13381 catggcggca acaagttgga atttggcagg atagacggct tggcgcgccg cgcgggaaat
    13441 gtggatttcg ccgttttcca acggttcgcg caaaacttcc aaaactttgc ggtcgaactc
    13501 gggcagctcg tccaaaaaca atacgccgtg gtgcgccaat gaaatctcgc ccggacgcgg
    13561 gtcggaaccg ccgccgacca tagccgccgc gctggcgctg tgatgcggac tgcggaaagg
    13621 acggttgctg tcgagttgtt gctggtggtt gggcaggagc gaacgcaatg cccaaacttc
    13681 taccaattcg tcttcggtca gcggcggcag gatgccgggc agccgttggg agagcataga
    13741 cttgcccgtt cccggcggac ccatcatcaa gaggctgtgt ccgcctgcgg cagcgatttc
    13801 caaagcaagg cgcgcggtgt gctgaccttt cacatcacac aaatcaggtt gtccgccatg
    13861 ttcagacggc atttgcggta cggaacattc ggtttgcgcc aaaggttcga tgccgttcaa
    13921 atgggcggcg acttcgccca aagagcgcgc gccgtaaacg gtaatgccgc gcatcacggc
    13981 ggcttgtcct gcattttctt caggcaaaac aaatgcacgt tttgcctgca taccctgcca
    14041 cgccatcgcc aacgcgccac gcacggggcg caacagcccc gacagtgcca attcccccgc
    14101 aaactcgtat tccgccagtt tttcgggcgc aacctgcccc gatgcagcaa ggatgccgat
    14161 tgcaatcggc aaatcgaaac gccccgactc tttgggcagg tcggcggggg cgaggttgac
    14221 ggtaattttt ttggcgggga attcaaaacc gctttgaata atggcggcac ggacgcggtc
    14281 gcgactttcc tttacttccg tatcgggcag cccgacgatg ttgaaatgcg gcaggccgtt
    14341 ggcaaggtgg gcttccactt cgaccaacgg cgcattcata ccgctcaagg cgcggctgta
    14401 aaccaaggca agcgacatat ttcagacggc cttattcgcc ggcttcggtt tgctgcctga
    14461 tttcggcgac ggcttcttcg gcagcggctt cagccgcttc caatgctgcc cgttcgggat
    14521 tttgcgcggc ttcgagtttt gccaaacgcg cttccaaagc cgccagtttg gtacgagttt
    14581 tgattaaaac ctgctgctgg atgtcgaatt cttcgcgcgt aaccagatcc atacggttga
    14641 acgcgccgcc cagcatcgcc ttaatatttt tttccacatc tttggcaggg ctgttggcga
    14701 tggtttcgct gattttcgaa ccgacttctt caaaaagctg cttgccgaac ataatctgta
    14761 tccttcctga acatatcaaa ttcaatcggc tattgtataa ggaaaaatgc cgtctgaaaa
    14821 cgggcggcgg ataatcggca aaacataccg cgcctccttt gcggttgcta aattacacct
    14881 caaaacacct gccgcctgaa aacggatttc atattccgcg caacgccaga ttacaagaca
    14941 ctacaaaaca atatactgtt ttaaatgatt tttccgacgc gcatcgtttc aaactcggct
    15001 ttttaagcca ttaagtgctt tgcaaacaac agaaattggg ttatgctgaa acggattatt
    15061 tacaatttca tatagtttta ttacatatct tattgtgatt gaagataatt tatccgaatt
    15121 cccctttcgg gtatccggat tttccgttgt acttttatta gaaaaacatt tcaggcgcaa
    15181 gttgcttgca atttcaaagc ctgtaattct gaagttttag acgaggaata gcacatgaat
    15241 cccatgtaca ttacttttgc aatctatctg gttgcagtcc ttctcatcgg gcttgccgcc
    15301 tatttttcca cgcgcaattt cgacgattat attttgggcg ggcgcagcct aggcccgttt
    15361 gttaccgcga tgtcggcagg cgcgtccgat atgtccggct ggctgctgat gggtctgccg
    15421 ggcgcgattt acctgagcgg tttgaatgag gcttggattg ccatcggcct cttggtcggc
    15481 gcgtatttca actggctctt ggtggcgggc cgcctgcgcg tgcataccga atacgccaac
    15541 aatgcgctga cgctgccgga ttatttcttc caccgcttcg gcgcgggcgg acacttgatg
    15601 aaagtggttt ccgcactgat tatcctgttc ttcttcacga tttattgcgc ctcgggcatt
    15661 gtggcgggcg caaccctgtt ccaaagcctg tttgaaggta tgacttacaa tcaggcaatg
    15721 tggctgggcg cgggcgcgac catcgcctat accttcttgg gcggcttctt ggcggtcagc
    15781 tggacggata cgttgcaggc ttctttgatg attttcgcgc tgattttaac gccggtgatg
    15841 gtctatctgg gcttgggcgg cgcggaacag atgtctgccg cgattcaaaa cgtcgccgca
    15901 ggcacgggca aagaatacgg cagcctgttt gccggtacga ccgtcatcgg catcatttcc
    15961 accgccgcat ggggcttggg ctacttcgga cagccgcaca ttttggcgcg ctttatggcc
    16021 gccgaaagcg cgaaatcgct ggtatccgca cgccgcatcg gtatgacttg gatggcgttg
    16081 tgccttgcgg gcgcggtagc ggtcggttat ttcggcattg cgtatttcgg tgcaaatccc
    16141 gacaaagttt cttctatgag cggcaaccac gaacgcatct tcatcgcgct ttccaccctg
    16201 ctgttcaacc cttggatcgc cggcattatt ttgagcgcga ttttggcggc ggtgatgtcc
    16261 accctgtcct gccagctttt ggtttgctcc agtgcgatta ccgaagactt ctacaaaggc
    16321 tttttgcgta aaaacgcgca acagtcggaa ctggtatggg tcggccgcct gatggtattg
    16381 gcgattgccg tgatttccat cctgattgcc tccaatcccg acagcaaagt attgggactg
    16441 gtgtcttatg cgtgggcagg tttcggtgcg gcattcggcc cgattgtgat tctgtccgta
    16501 ttgtggaaac gcatcactgc ctacggcgcg ttgtcgggta tggttgcagg tgccttaacc
    16561 gtagtcgtat gggcggaatg ggtaaaaaaa ccggctcaag cagcaggcga aagcggcttg
    16621 ttgacgatgt acgaaatcgt cccgggcttt attgtttgct taatcattgc cgtgttggtt
    16681 tccctgttca acaaagaacc ttcgcgcgaa atccaagaac gctttgaaaa agccgacgca
    16741 gattaccgcg ccgcccgata aacccctttc agacggcacg gcatcccggt tcgtgccgtc
    16801 tgaacccgtt tccgggtcaa acacaaataa accttgcctg catacaggcc ttatttgtgt
    16861 tttacccgtt tcattttcaa agaaacggaa gcacctaccc tgcccgcatt tgaagccttg
    16921 tatttcatct gcggatttat tacgatatta ccgtattcag gccgcaccga tgccgcctgc
    16981 cccccgaaaa actttggaga atccaaaaaa tgtttcattt tgcatttccg gcacaaactg
    17041 ccctgcgcca agcgataacc gatgcctacc gccgtaatga aatcgaagcc gtacaggata
    17101 tgttgcaacg tgcacagatg agcgacgaag agcgcaacgc cgcctccgag cttgcccgcc
    17161 gtttggttac ccaagtccgc gccggccgca ccaaagccgg cggcgtagat gcgctgatgc
    17221 acgagttttc actctccagc gaagaaggca tcgcgctgat gtgtctggca gaagccctgc
    17281 tgcgtatccc cgacaacgcc acgcgcgacc gcctgattgc cgacaagatt tcagacggca
    17341 actggaaaag ccatttgaac aacagccctt ccctcttcgt caacgccgcc gcctggggcc
    17401 tgctgattac cggcaaactg accgccacaa acgacagaca aatgagttcc gcactcagcc
    17461 gcctgatcag caaaggcggc gcaccgctca tccgccaagg cgtaaattac gccatgcgcc
    17521 tgttgggcaa acagttcgta accggacaga ccattgaaga agccctgcaa aacggcaaag
    17581 aacgcgaaaa aatgggctac cgcttctcct tcgatatgtt gggcgaagcc gcctacaccc
    17641 aagccgatgc cgaccgctac taccgcgact atgtcgaagc catccacgcc atcggcaaag
    17701 atgcggcagg acaaggcgtt tacgaaggca acggcatttc cgtcaaactt tccgccatcc
    17761 atccgcgcta ctcgcgcgcc caacacggcc gtgtgatggg cgaactgttg ccgcgcctga
    17821 aagagctgtt ccttttgggt aaaaaatacg atatcggtat caacatcgat gccgaagaag
    17881 ccaaccgtct ggagctgtct ttggatttga tggaggcttt ggtttcagac cctgacttgg
    17941 ctggctacaa aggtatcggt ttcgttgtcc aagcctacca aaaacgttgt ccgttcgtta
    18001 tcgactacct gattgaccta gcccgccgca acaaccaaaa actgatgatc cgtttggtta
    18061 aaggtgcata ctgggacagc gaaatcaaat gggcgcaagt ggacggcttg aacggctatc
    18121 cgacctacac ccgcaaagtc cacaccgaca tctcctacct cgcctgcgcg cgcaaactgc
    18181 tttccgcgca agacgcggta ttcccgcaat ttgccaccca caacgcctac actttgggcg
    18241 caatctacca aatgggtaaa ggcaaagatt ttgaacacca atgcctgcac ggtatgggcg
    18301 aaaccctgta cgaccaagtc gtcggcccgc aaaacttagg ccgccgcgtg cgcgtgtacg
    18361 ccccggtcgg cacacacgaa accctgctcg cctacttggt gcgccgcctg ttggaaaacg
    18421 gcgcgaactc gtctttcgtc aaccaaatcg tcgatgaaaa catcagcatc gacacgctca
    18481 tccgcagccc gttcgacacc atcgccgaac aaggcatcca cctgcacaac gccctgccgc
    18541 tgccgcgtga tttgtacgga aaaggccgtc tgaattccca aggcgtcgat ttcagcaacg
    18601 aaaccgtgtt acaacagctt caagaacaga tgaacaaagc cgccgcgcaa gacttccacg
    18661 ccgcctccat cgtcaacggc aaagcccgcg atgtcggcga agcgcaaccg attaaaaacc
    18721 ctgccgacca cggagatgtt gtcggtacgg tcagctttgc cgatgccgcg cttgcccaag
    18781 aagcggttgg tgcagccgtt gccgcgttcc ccgaatggag tgcgacacct gccgccgaac
    18841 gcgccgcctg cctgcgccgt ttcgccgacc tgctcgaaca gcacaccccc gcgctgatga
    18901 tgcttgccgt gcgcgaagcg ggcaaaacac tgaacaacgc cattgccgaa gtgcgcgaag
    18961 ccgtcgattt ctgccgctac tacgcaaacg aagccgaaca caccctgcct caagacgcaa
    19021 aagccgtcgg cgcgattgtc gccatcagcc cgtggaactt cccgctcgcc atctttaccg
    19081 gcgaagtcgt gtccgcattg gcggcaggca acaccgtcat cgccaaaccc gccgaacaaa
    19141 ccagcctgat tgccggttat gccgtttccc tcatgcacga agccggcatc ccgacttccg
    19201 ccctgcaact cgtcctcggc gcaggcgacg tgggtgcggc attgaccaac gatgcccgca
    19261 tcggcggcgt gattttcacc ggctcgaccg aagtggcgcg cctgatcaac aaagcccttg
    19321 ccaaacgcgg cgacaatccc gtcctgattg ccgaaaccgg cggacaaaac gccatgattg
    19381 tcgattccac cgcacttgcc gaacaagtct gcgccgacgt attgaactcc gccttcgaca
    19441 gcgcgggaca acgctgctcc gccctgcgta tcttgtgcgt ccaagaagac gttgccgacc
    19501 gtatgctcga catgatcaaa ggcgcgatgg acgaactcgt cgtcggcaaa ccgattcagc
    19561 tcactaccga tgtcggcccc gtcatcgatg ccgaagcaca gcaaaacctg ttgaaccaca
    19621 tcaacaaaat gaaaggcgtt gccaagtctt accacgaagt caaaaccgct gccgatgtcg
    19681 attccgaaaa atccacgttc gtccgcccca tcctgtttga attgaacaac ctcaacgaac
    19741 tgcaacgcga agtcttcggt cccgtcctgc acgtcgtccg ctaccgcgcc gacaaactcg
    19801 acagcgtcat cgaccaaatc aacagcaaag gctacgccct gacccacggc gtacacagcc
    19861 gcatcgaagg cacagtacgc cacatccgca gccgcatcga agccggcaac gtttacgtca
    19921 accgcaacat cgtcggcgca gtcgtcggcg tacagccctt cggcggacac ggtctgtccg
    19981 gcacaggccc caaagcaggc ggttcgttct acctgcaaaa actgacccgc atccccgaat
    20041 gggttgcccc gaccctgagc caaatcggac aggcagacga agccgcgctc aaacgcctcg
    20101 aagcactggt tcacaaactg ccgttcaacg ccgaagagaa aaaagccgca gcggccgctt
    20161 tgggacacgc ccgcatccgc accctgcgcc gtgccgaaac cgtccttacc ggcccgaccg
    20221 gcgagcgcaa cagcatctca tggcacgcgc caaaacgcgt ctgggtacac ggcggcagcc
    20281 tcctgcaagc cttctgcgcc ctaaccgaac ttgccgcctc cggcgtacag gcagtggtcg
    20341 aacccgacag cccgctggct tcctacaccg ccgacttgga aggtctgctg ctggtcaacg
    20401 gcaaacccga aaccgccgac atcagccacg ttgccgccct gtcgcctttg gacagcgcgc
    20461 gcaaacagga acttgccgcc cacgacggcg cactcatccg cgtcctccct tcggaaaacg
    20521 gactcgacat cctgcaagtt tttgaagaaa tctcttgcag cgtcaacacc acagccgccg
    20581 gcggcaacgc cagcctgatg gcggttgccg actgattttg ccaaaaccgc ccgagcgcgg
    20641 cccgtgaacc aatgccgtct gaaaaccttt cagacggcat ttttatagtg
//
LOCUS       DAXHKX010000018        20297 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHKX010000018 DAXHKX010000000
VERSION     DAXHKX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20297
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.018"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(232..307)
                     /locus_tag="ACFMWZ_000569"
     tRNA            complement(232..307)
                     /locus_tag="ACFMWZ_000569"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Asn,seq:gtt)
     gene            complement(400..759)
                     /locus_tag="ACFMWZ_000570"
     CDS             complement(400..759)
                     /locus_tag="ACFMWZ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC5934568.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(782..1426)
                     /locus_tag="ACFMWZ_000571"
     CDS             complement(782..1426)
                     /locus_tag="ACFMWZ_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC5934569.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRM"
     gene            complement(1428..1964)
                     /locus_tag="ACFMWZ_000572"
     CDS             complement(1428..1964)
                     /locus_tag="ACFMWZ_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC5934570.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1976..3073)
                     /locus_tag="ACFMWZ_000573"
     CDS             complement(1976..3073)
                     /locus_tag="ACFMWZ_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC5934571.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLASVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3076..4512)
                     /locus_tag="ACFMWZ_000574"
     CDS             complement(3076..4512)
                     /locus_tag="ACFMWZ_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC5934572.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4732..5514)
                     /gene="bioC"
                     /locus_tag="ACFMWZ_000575"
     CDS             complement(4732..5514)
                     /gene="bioC"
                     /locus_tag="ACFMWZ_000575"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC5934573.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5502..6149)
                     /locus_tag="ACFMWZ_000576"
     CDS             complement(5502..6149)
                     /locus_tag="ACFMWZ_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC5934574.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6159..7301)
                     /gene="bioF"
                     /locus_tag="ACFMWZ_000577"
     CDS             complement(6159..7301)
                     /gene="bioF"
                     /locus_tag="ACFMWZ_000577"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC5934575.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            complement(7323..7460)
                     /locus_tag="ACFMWZ_000578"
     CDS             complement(7323..7460)
                     /locus_tag="ACFMWZ_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC5934576.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            7524..8600
                     /locus_tag="ACFMWZ_000579"
     CDS             7524..8600
                     /locus_tag="ACFMWZ_000579"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC5934577.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8807..10273)
                     /locus_tag="ACFMWZ_000580"
     CDS             complement(8807..10273)
                     /locus_tag="ACFMWZ_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC5934578.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10471..11394)
                     /gene="speB"
                     /locus_tag="ACFMWZ_000581"
     CDS             complement(10471..11394)
                     /gene="speB"
                     /locus_tag="ACFMWZ_000581"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC5934579.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11493..13385)
                     /gene="speA"
                     /locus_tag="ACFMWZ_000582"
     CDS             complement(11493..13385)
                     /gene="speA"
                     /locus_tag="ACFMWZ_000582"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC5934580.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYASTVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13552..13836)
                     /locus_tag="ACFMWZ_000583"
     CDS             complement(13552..13836)
                     /locus_tag="ACFMWZ_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC5934581.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13987..15795)
                     /gene="aspS"
                     /locus_tag="ACFMWZ_000584"
     CDS             complement(13987..15795)
                     /gene="aspS"
                     /locus_tag="ACFMWZ_000584"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC5934582.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGGFPRMPYAEA
                     MFYYGSDKPDMRIGLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15853..16554)
                     /locus_tag="ACFMWZ_000585"
     CDS             complement(15853..16554)
                     /locus_tag="ACFMWZ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC5934583.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16697..17845)
                     /locus_tag="ACFMWZ_000586"
     CDS             complement(16697..17845)
                     /locus_tag="ACFMWZ_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC5934584.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(18005..18268)
                     /gene="rpsT"
                     /locus_tag="ACFMWZ_000587"
     CDS             complement(18005..18268)
                     /gene="rpsT"
                     /locus_tag="ACFMWZ_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC5934585.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18571..19713
                     /locus_tag="ACFMWZ_000588"
     CDS             18571..19713
                     /locus_tag="ACFMWZ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC5934586.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5228 a         5245 c         4705 g         5119 t
ORIGIN      
        1 agaatcgttc tctttgagct aaggcgagcc aatgccgtac tggtttttgt taatccacta
       61 taaaatcgag tttatcctag ttgtccagga cagcccctat tttttcataa ccatcacgaa
      121 aggaattttg caaagatttt attccatctc aaaacaatca tctcaaaaat gcgtttctga
      181 ccgccggtaa aacaaaaccc tctaagaaaa tacttagagg gttttgaaat ttggctcccc
      241 gacctgggct cgaaccaggg acctgcggat taacagtccg tcgctctacc gactgagcta
      301 tcggggaata agcgcgaata ataaaacaga aataccgaac gtgtcaatat aaattgacaa
      361 tcgattgcac tttatctgca agcccgcaat ctttatatgt tatatggctt ccgcaatatg
      421 ccttgccgca cgtttggcat ccaataggat taaaccaagt ctgccggttt cttttgccac
      481 cagcaccaac acggcatctt tacccgcctg acttaaaagg atatagcctg attttccttt
      541 aatcatcact tgttccaatt ccccgcaggc gagttcctgc accgagcgac tccccaaagc
      601 aagcaaagtg gcagaaatcg cccctaccct gtccgaattc aaatgtgaag gaagcattgt
      661 cgccatcggc aatccgtcgg ttgagataac ggcagacgcg ataatatccg tagatgtatt
      721 gtttaaatct tcaaggattg aaatcaataa ttgctgcata acccctcctc tctcccaagt
      781 tttacatgcg gttgctgtaa cggcggtata aaatccttac caaagtaaca aatgcctctt
      841 tgcccaaatc gggaatgccg ccgataacca aaataaattt ggttgaaccg atatacaatg
      901 ggaaaaatgt caattcgctc tgaccggaag gatcgcaaac gccccaagcg ttattgttga
      961 tatacaggtt gttcttaatc agcagccggt atttcttttc catctgtgcg acttctgccg
     1021 ccaacaaccc caactcttcc gccgcctcat gatggaaatt ggcgttggca agatacagac
     1081 cgttccgatc gaccaataac gccttaccgc tgccggacaa ttgttccatc agcaacggca
     1141 attgctcgtc cgacaaattg atgccgtctg aatgaccgtt ttcatcgcca tagaggaatt
     1201 cgagtttttg caaacggtac aacaggttca aagcggtatc gatgtcggcg gtgtccgccc
     1261 aggtaagcag cttctcactg ctgaccattt cgtccgcatc cgctttcaac aggctgtgca
     1321 acaaagtttt accggcactg ggggcatcgc tggatacggc ataaaatgca ccggcaggag
     1381 tcaggcgggg atataaattt gcttgtagtg aaagtgttga ttccatatta aacctccagt
     1441 cccggatcaa tagaaaataa cattgcgcta accaattgtt ttacgtcatc ttccttacgg
     1501 gcatcaattt caaaaaccgg aacattaaga ttatgttttg caagatattt gtgatacacg
     1561 tcgataccgg gctgagagcg tatatccatc ttggtaatac cgacaacgac gggtgccttc
     1621 tccagcagcc ctcgaaacga atgtaaaaag aattccaaat ctttcaacgg attggttcgg
     1681 gcattatcta aaagcaagac caaacccata ctgccttggc ttaagatttc ccacataaag
     1741 ttgaaccgtt cctgaccggg cgtaccatat aaatggactt tggtatcctc atccaagctg
     1801 atggccccgt agtccatcgc cactgtcgta ttccttttcc tatccaaagt catatcggat
     1861 gcggaagcat cggtctgaac gagtgcttcg tccgaaatag ccgcaatggc agtggttttc
     1921 cctacgccga caggtcctgt gaaaataatt ttattttctc tcatctctcc gcctcttagc
     1981 tgcccaacag ttttttcatc agcctttgca gaaggccgcg cgactgggat tgggatggtg
     2041 ttgtgatttt ttccgctttt ttcatcattt cactatcaga ggcagaatcg gctccgatat
     2101 ttattttatc cgccatatcg gagtatgcct gttgtgaaac cgtttttaaa tctaccgaca
     2161 aaaacccggt tgtataggtt gccgcaagat aattcagaat atcattgagg tttaaaggca
     2221 tcactttata caacacgtta aggttgacgg atgccttggt cagaaatgcc gacaagcgta
     2281 tcgaccccgg cacacttgcc aaccgggtca ggtttggcca agatttcaac gtaaacggag
     2341 tatcgggaga aatcggataa atcaacctgc cctgcgccgt ccaaatggaa aactgccaca
     2401 tacaggacat aatgcctact ttagcctttt cgcgccattg cgggttatcg ggaacagtct
     2461 tgcagctgac ctgcaaattt tcgtctttgc acaattcttc gagtttttgc acactttctg
     2521 tcagcaaaac ccgttgtatc gaggggaaaa caataaggac cggcttattt ccatgcaaga
     2581 tagcgatgtc ctgcctgttc ttttccgcaa accgcaacgc ccccaataat cctttattcg
     2641 ggttaaactg gcgtatcgta accgtacgct gcacattccc gttatttttc gccgaccggt
     2701 ctgcgggtgc aataaacgat ttcccataaa cattctcgcc ctgcaacaat ttgcggagca
     2761 taggaaacaa tgtttcaaac cgaatcggtt tgggcaggta gggaacttca gaatcgggaa
     2821 ctttctccga acagacggcg acgggtatat ccttataacg ctcggcaagc tctttccaaa
     2881 gttcaaaacc gccctcggca tcggtatccg ccaaaaccaa atcgggcacg gcactgccgt
     2941 ctgaagggga tactgtttca taacgggtgg tattgtgcat tttgaatgcc attttgaaaa
     3001 cggattcctg ctgcgccgtc atccccgcca acattacgcg tactgtttta attttcggca
     3061 gttgaacttc catttttatt ttccgtaccg ttttattttt ttaatatttt gattcatacg
     3121 ctgcagcagc cggctcatca gcataacgac ctcttcagga agcctgtccg cacgttccct
     3181 caataccctt aaaaactgcc ccaacctatc ccaatcttca gtacgttcat aaatatcgat
     3241 caacgtaata taaagctggg actcgtcggg atatttcaat accgcctgct ccaacacatc
     3301 cattgccgct tcaatctgac catacatcag caacgactct acttctttaa ccgcatcgtc
     3361 tgccggagac gaaccggtgt tgatcaacga agaatcttga agcaccaaat cccgatgttg
     3421 cgatttgaat ttctgtatat ttttcggcag atacccgtgc cccataccga tgtctttgat
     3481 ttgccggtcg ttcggccctt tttccaaatc atcgaaaact tcatggtaac ccaagctgta
     3541 cccccacccc agcatccgct ctttaacctg cctgccgtag ttgcccaacg tttggtaaag
     3601 tttccacaaa tgtccggcaa aacggtctat gtccgcgtgt tggtaatcga gtttcaacgc
     3661 atcgataatc aggtttgcag gtttttcgga agtttggatg gcacggtcgt attgtttcga
     3721 tgccgtttca tagctgactt tgtctttaag gattttcgca ccttgatcgg cacggaccaa
     3781 accggcaatc gcaccgattt cctcttgact gattccggac acgtcttttt tgccccgcac
     3841 aatcgggatg cgcttgattt cttcggcttc ataagcctta ccgccggcat ccggtggggg
     3901 cgatgcggaa gctgccgtcg cagaaccgcc ttccgcacgt ttttcaatct cctgagtacc
     3961 ccatcccaaa ccctcttctg ccaagacgcg gatacgcaaa tggtttgaat cgcgctgtaa
     4021 tgcctgttcg atatattttg ccaaaagttc ggaaggaatc agtttgccgt atttttgcag
     4081 attgtctgcc aaaacatcga catcccccac ttcgagattg atatcgagca gctcgcggat
     4141 aaggttttca ggtttcgctt caccatccgg aatgccgtcc agataggcag ccaaagattc
     4201 ggcagccttg ccctgataac cgaattgctt ataaacctga tactccgtaa gcggatcgac
     4261 ttcttgcgcg gatacggcgg cggatgcggt ctcggcactt tcgttccaag accagtcggg
     4321 ttgcgtgccg tctgaaaccg attgtccaat ctgatcgacc cagtccgaat cattgcttcc
     4381 gggtgtctgc ccctgcgtat tgccgacacc ttgggatttg cgcttcggtg ttttcttacc
     4441 ctgcttcgca cgcataacca aaagcatcag caataccgcc agtgccaaac cgataattaa
     4501 tgagttttcc aaaggatact cccgataccg taacgagcgg atagctgacc gcctgcaacg
     4561 gccattgtcc gcttttatta ttaacgattt actgaaatag acttgtaagt tttaaatcat
     4621 accataattt aacgtttaac aatatgcctt ctgcacaagc ctcgccatat taccttttac
     4681 ccacatcagt atcaataccc gatataaaaa taactttgcc cataagctgc cttattgcct
     4741 gcccgccgca gagtagcgcg cgataaaaaa taacggatga taagtcaggt gcactttgcc
     4801 cgacggcata ctgaacgccg acaagtaatc gccaataaat ccattgagat tttttcttgt
     4861 ccaatttttt tggttcgtgg ctgttacgcc tgtatattta aggtgtttga gtacatctaa
     4921 cggcgtatca aagtctagta ttaccttaaa atcctcacac cataaaagct caaaatcttt
     4981 ggctaaccaa gtctgccatt gggataaagt cgggtaattt aagcctatat ttgtaatttg
     5041 gcggacttct tttaaattgt ctttgccaaa ggttgcaacc gccaataatc cgtttgcttt
     5101 caagcctgtt ttgcaatggg cgataaaagc gtcgggttga tgaaaccatt gcacggcaga
     5161 tgcgcttgcg attaagtcaa attgtcgttg aaaaggaaag ttttccgcat cgccgcaata
     5221 aaaatcaaag gattgcggca gtttttcagc cagttggggc tgcacatcgc acaaatcatt
     5281 aaataaccaa taattcgctg aaatctgttt ttgcagcaag gcactcaaca ttcctgagcc
     5341 gcaacccaat tccaacacgt tttccaatgg catatccggc aaataatctt gcaaatgcgc
     5401 cattagatta atcgtcattt tttgttggat taaggcgtgc cggtcataat cgtttaatgc
     5461 tttttggaaa gcctgccgaa tgcgcgattt atttatggca gtcagtgatt ccatagtgcc
     5521 gaccaatggg tgaatcttga aaacaggtaa tgtccgccgt caatttcccg aacggtgcaa
     5581 cgcggtgtcc aatatcggtg ctgattggca ggcataaaaa ttttatcgcc cgatccgacc
     5641 aaggcatttg tccagcgaat caaatctgcg cgcctatctt gcccgatcat cgcaaaaagt
     5701 gcgataagtt cttgatgaat ttcgtcaaac ggacgggcgg gaaattgttg gtaatcttca
     5761 aaagatgctt tatcgccaca cattctgcgt tcaaatttta aacgggtgtt ttccgtgagg
     5821 ttctccaatg tgcctttaaa aacggcgcaa gggataccga aattatcatc gcaaggcaaa
     5881 cctgtgccat tcactgccgt tgcggatttt aatcttattc cttgcaatgc cctctctgcc
     5941 gcccaaacgc ccattgacca cgccaccaaa cggatgtgcc gataggcgga aaaatcaaaa
     6001 tccaaattta aatcttgata atcatagcaa atcaataaat cgtgattttc cggcaaaatc
     6061 aaatgattta cagcatcggg cggcgttccc caacctgcaa aatacaggat taaatgttcg
     6121 ccttgatgat tgtaaaattt tgttttcata tcatatcctt acaggcacgc cgcaaactgc
     6181 cgcacttcat ccattgtcat atctgccgtc aaagacaatc gtattctgga tgtacctttt
     6241 ggtactgtcg gtggtctgat gggcaggcaa taataaccct gcctttgcag gtattcagct
     6301 ttggcaaggg tggcttcatt cccgcctaaa atatagggga cgatacaggt ttggctcggc
     6361 attatttgcg tccgatgcgc cacttcccgc cgtaaaaatg cgcttaactg ctcaagatgg
     6421 cttctttctt ttgagaattg cggcaatcgt tcaaaaataa aataagtcca agccacatta
     6481 aacggcggca atgcggttga aaaaatcaat gggcgcattt gattaatcaa acattctttc
     6541 aatacttggt tgcagacggc atacgccccc accgaggcta aggctttacc gaaagtgccg
     6601 accaataaat caatctcggc aatcaaatta tcccgttcgg caatccccaa tccgttttgc
     6661 ccataaacac cgattgcgtg agcttcatcc acataaagat aagtattggg aaactgtttt
     6721 tttaattgga caagctgttt caaatccgcc acatcgccgt ccatactgaa aacagattcg
     6781 gtaacgataa aagtgcggtc aaattttccg acgttttttt caagcagatt tttcaaatgt
     6841 tcataatcat tatgacgata acggaaaaac gcacaccggc tcaaacggat gccgtcaatc
     6901 atactggcgt gaacaaattt atctgccaaa atcaaacttt tcgtcgtcgt caaagcaggc
     6961 aaaataccga gattggcgtg atagccgctg ttgaacaata acgcgctttc ccgttggaaa
     7021 cgttgtgcga caagctcttc caaatcggta taaataggaa agttgcccgt taataaacgc
     7081 gatgaagaac tggtaaaaga gggaaaatta ccgccgtatt gctgcaaaaa agaccggcgc
     7141 aagttttcat ctgatgccaa acccaaataa tcattagacg acatattcag cattttgcgg
     7201 ttttcccgcg taatataccg cccttgatga atcaaatccg gaatcgaacg atattggttt
     7261 tgcgcgccga gttgttcaag ctgttgttta aaaaccttca ttatgatgta aatattcctg
     7321 agttaaagcc tgaaccaacc cagcatcaaa caatcaagta atgccctttt aataaaaacc
     7381 gttttgccgc cgacctttgc cacacccctg ccttcataat ccaaggcgga aatttccgcg
     7441 acatttgttt ccgttgccat tacccaatcc gtccgcactg taccgaagcg catattttcg
     7501 cacaaattgg tacggtttca ggtatgattt gccgaaaacc tatcggacac acaccatatt
     7561 ttgatatgaa taaaacaatc tgccgcactg ctgcactatt gataagcggt ttttcatacg
     7621 caaacaccgt cattcccgat gtctcccccg tcgcacaagg tcaacatgtc ttcattaaca
     7681 tccctcagca acgcctgttc ctctacaccg acggcaaact gaccaaggtt tatcccgttg
     7741 cagtaggtcg ggcgatgacg caaaccaatc taggcgaaca taaaatcggt gcgaaagcct
     7801 ataaccctgt ttggtacatc cccaaatcca tacagaaaga acgcggcgac ggcgtaaaaa
     7861 ccatagccgc cggcccggac aaccctttgg ggccggtttt tgtccgctta ggcgacccta
     7921 aactcggttt gggcatacac gggaccaatg cgccggccag cgtccccggg gttcggagtc
     7981 acggctgcgt ccgcatgaaa tcgcccgacg cgctcgagtt tgccaaaacc atcgccagcg
     8041 gctcgcccgc ctccgtcatc tatcaaatgg cgggtctcaa tgaagatgcg gatcgcaacc
     8101 tgtggcttgc cgccttccgc gacccttacg gtaagaacaa ccttgacatc gcctctctga
     8161 aaaaaagtat tgcgcaatgg gcaaaaacac agggtaaaac catcgcgccc gagaaagtcg
     8221 atgctgtact caaagaccgc accggatcgg ccgtctgcct gacctgcggc aaaaacggca
     8281 agatgaagat gccgctcaaa tcgctggcgt ggatacaggg ttcttcctca tacagccaag
     8341 ctgaagtcat tgaacaaact gaggaaacca actctgccga aatatccgag acacgcacgc
     8401 ccgaagtgcc tgatgtacac acgcccgagg cacaaccgca tttaaatacc caatccgacg
     8461 gcacgccgac tgcctatacc gaaccggctg ccgattcatc gccgcaagta gaaacacctg
     8521 atcaggctgc ttccgagccg gttgatgtat tattttcaat agatgtgata cggcagggaa
     8581 atttgcgttt aggtaattga ataaccttct gattatttaa tgcttatgtt tatcagaagt
     8641 tgataggcgg tttggttttt gtttggcttt tcctacaccg ccgcctgccg ctttctgcaa
     8701 cattcaagcg cacaaatatg ccatctgaag gctttagacg gcatatttca cgataatagt
     8761 cgcccaatca aataaacaca gattggaaat ttattttatt tttcccttac caatacccca
     8821 gaactttcca ccaaatgctg ccgataacga aaaaaatcag aaaattgact acgctcatga
     8881 taaaacccgc cttccaccat tctcccattg tggtgtagcc cgaaccgaaa atcacaggcg
     8941 aagtaccggt cgcataatga gtgagggtca tcataatgtt agatgcggcc gccatcatca
     9001 gcgcggtcgg catcgccggg gcattcagtg aaacggcagc agcgaaaaat gcgccgaaca
     9061 tagcggtaat atgtgcagta gtactggcaa acatataatg cgcatacata taagcaagca
     9121 cgaggattac gcccgcagcc gtgccgctaa cgcccaaacc gccgacactt tccgccaaca
     9181 ctccggagaa ccatttaatc agtccgagtt tatttaaaaa tgcggccatc ataatcaatg
     9241 cgccaaacca aataatcgta tcccacgcgc ttttttcttt caaaacatcg tcccaagtca
     9301 atacaccgga aagcaaaagc aggcttaatc cgataaatgc ggtggcggtg gcgttgatac
     9361 taaaagcgtg attgccggta ataagggcgg gaacatctgc ccacaacagc aacaagatac
     9421 cgaaaatgac cgccataatg atttcgtctg ccgacatttt acccatctcc ctcagacggt
     9481 ctttggcaaa ttgaacggca ttgggcgttt ctttaatttc aggcggatac aaaaaatata
     9541 aaatcaaagg cataacgaaa aaggcgataa cgccgggaac agccattgcc cacgcccacg
     9601 ccccccaaga aagacggaaa ctactgccta aattttcggc aatcaagttg acgattaaag
     9661 ggttgggggc agttgcagta ataaacatag ccgacgaaat gggattggaa tgatagttga
     9721 ccaaagccaa atatttaccc atcttgcctt ctgtgccttt tgcgggattg gagccgtaac
     9781 tgccggcaat cgactgcata atcggatgta taatgccgcc gccgcgcgcg gtattggaag
     9841 gggtaacggg agccagcagc agttcggaaa gagcgagact gtaaccgatg cccagcgttt
     9901 ttcttccaaa aacggcgata aacaaatatc cgatacgcat ccccagccct gttttgagca
     9961 aaccgcgcga aatcataact gcgatggcaa tcagccaaat caacggattg gcgaacgcac
    10021 tcaacgcatc gctcatcgcc gcgcccggtt tgtcggcggt tacgccggtt actgcgacca
    10081 acccgacggc aataatcgac agcgcgccca acggcatggc cttgccgata atggcggcaa
    10141 tcacaccgac aaacatggcc agcagcgtcc aagcctgagg cttgaccccg tcgggtacgg
    10201 gcagtgccaa aaccagggcg cacaatactg cggcaatggc gaggggtatc ggtttgaaac
    10261 ccaatttcat catattgacc tccgtaaaaa agaccgtccc gaaaaatcgg aaaaataata
    10321 tttaactaat tgttttataa gatatattcc gatatttcac cgtctttccg atatgcggct
    10381 ccgggcaact tttgtttcag tatttgaatt ttcattagac tgaatacgcc gtttgaacgg
    10441 cacggcgaaa acccggggga tgccggacgt tcagtccttt ttcgcacctt gaaggtaaag
    10501 catttccaag gcaattgtgg cgccggccaa agcggtaatg tcggattggt cgtaagaggg
    10561 ggcaacttct acaacatcca taccgacgat gtcgagatcc gtcagcccac gtaggatttt
    10621 taatgccctg tcgctgctca agccgccgca tacgggcgta ccggtcccgg gggcgaacga
    10681 cgggtcgagg cagtctatgt cgaaagtcag gtaaacgggc atattgccga cggtttcttt
    10741 gattttacgg acggtctctt caacactgtc ttcattaact tggggggcgg tcaacacagt
    10801 aaaaggcaat tttttactgt gttcggtacg tatgccgatt tgtacggaac gggacgggtc
    10861 gatgaggcct tccttggggg cggtatagaa catcgtaccg tggtcgtatt cgctgccgtt
    10921 gtcgtaggtg tcggtgtgcg cgtcaaaatg aatcagtgcg agtttgccga aatagcgggc
    10981 gtgggcgcgc aacaacggga gggtaatgaa atggtcgccg cccaaactca aacagcgttt
    11041 gccggaagaa agtaatttgc cggcgtgcgc ttccattttt tcgacaaaat ccctgctgtc
    11101 gccaaaagaa aaaaccaagt cgccgcaatc aataatgttc aggcgttcgc gcacatcaaa
    11161 tgtccatgga aacctgcggt gctcccaagc gaggttgacg gaggcgcgcc ggatggcttc
    11221 aggaccgaaa cgcgcgccgg aacgccctga aaccgccata tcataaggca cgccggtaat
    11281 aacccaatcg gcatgacttt catacggcat aaaattaagc ggcaggcgca aaaacccgaa
    11341 attattggaa acgagggagt tgtcggtttg tcctgccagt gtgctgtatt gcatcgtaat
    11401 gattccttgt aattggtttc aatcggtcgt gatgattggt gtttgagtaa gaaaatcggg
    11461 cttcagacga catatccgat gccttgatgc gtctattcgt cttccaaata ggtataacca
    11521 ttaagccccg cttcgagttc ttttaagaaa gacatagcct gcgaggcagg aaggtctgaa
    11581 tgttcgattt gttcgcgata gcgtttcatc agctctttcg gatcttgata aacgtattcg
    11641 agcatatcgg caacggtgtt tccttcatcg taatcgatga cggtaaattg tccgtcttcc
    11701 cctacaacaa catcggcagt ggcagtgtcg ccgaaaagat tgtgcatatt gccgagtatt
    11761 tcctgatatg ctcccaccat aaaaaagcct aaaagcggcg gctcttcttc gggataatca
    11821 ggcataggca tcgtaccggc gatgccgtct ccgtcgatgt agtggtcaat cgtaccgtct
    11881 gaatcgcagg taatgtccaa caacacggcg cggcgcgcaa tcggttcatt caaaccggta
    11941 atgggacaaa cagggaaaag ttgatctatg ccccaagcat cgggcaaaga ttggaagagt
    12001 gagaaattga catacagctt atcggcaaaa cgttcttgca attcgtcaat aatggttcgg
    12061 tgagaccggt gtttttcatt aaacaattcg ccgacttcat gacagatatt taaatacagt
    12121 tgctccgccc acgcacgttg cgccaaactc aacagcccga cattatactg attatgcaca
    12181 tcggcaagat caaactgccc ttcgtgtatc cagctgcgta aggaacgttt ttcccgcgag
    12241 gcggaaatat ccgtccaagt ttcccacata ctgtgcaaca cacgcggtgc ttcgggcgat
    12301 ggcgcatcca gccggcgcgg tttgtaacgt tcaacgccta taacattagc aaccaaaacg
    12361 gcgtgatgtg cggtaatgcc gcgcccgctc tcggtaatga ttgtcggatg cggcagcccg
    12421 tgttcgagac aagcctggct gatgccccat acgactgtgg aggcatattc gttgaggctg
    12481 taattaacgg aacaatccga ttgtgtgcgg tttccttcgt aatccacgcc aagcccgccg
    12541 cctacatcaa aacagcggat atttaccccc agtttgtgca actcaacata aaaccgagcc
    12601 gattcgtgta cacctgtggc aacatcacgg atgttcccaa gctgcgagcc taaatggaaa
    12661 tgcaaaagct gcaggcagtc cagcctgttt ttttgtttca aaatatcgac cagttgcaaa
    12721 acttgggaag ccgacaagcc gaattttgat ttttccccac ccgaagactg ccattttccc
    12781 gaaccttggg aagccagtct ggcgcgcaca cccaaacggg gcttgatgcc gagtttttcc
    12841 gcctcttcca ataccatttg tatttcggac agcttctcaa tcaccaaata aacctgatgc
    12901 cccagttttt cgcccatcaa ggcgaaacgg atatattcac ggtctttata gccgttgcag
    12961 acgattaatg tttgccgggt gccggcgtgt gccaaaaccg ccatcagttc ggctttagaa
    13021 ccagcttcca aaccatgcgg ttgtccgctt gacataagcg attcgatgac gcggcggtgt
    13081 tggttgacct tgatagggta aaccaaacaa taaccgccct tatagccgca ctcttcccgt
    13141 gccgtctgaa aggcgcggtt aatgtcgcgg aggcggtgtt cgaggatttg cggaaaacaa
    13201 aacaaaacag gcaggcgcgc ctgatgtttt tgttgcacgg cttcagtcag tttttgcagt
    13261 gaaacagttt gattgtgttg cgagggattg gggcggacga tgatttcgcc ggaatcgtca
    13321 acatcataat aacctatgcc ccaatgatta atgttgcaca cttcacggat ggtaaggata
    13381 ggcataataa acctgctccg tctgtcgtgt tgaaaaggaa tgattataac aaatcagcgt
    13441 gaaatgtcat ttttttaata agaaaagcct gcctcatacc tgatgagaaa caggcaaaat
    13501 gccgtctgaa cgcttcagac ggcattttgg ttcagctttc catcaaagga atcagcgatc
    13561 gagctgctct ttgatgattt tcaggtcggg gtaagacaac acgatgtcgt attcgcggcc
    13621 gtctttatag gcttccactt ccaaaacagg tttgccccaa tggtcgtcgg catcgacatc
    13681 gtgaacctga taaccgcgct gttccaacat ttttacggct tttgtgcggt tttgttcaaa
    13741 atggggatca ccgtaaatct gacgctcggc agaatcgtcg gcaaatgcgg ttgcggcact
    13801 cagggaaaca acggcagcca ataacagttt tttcattttc agtcctttct taatcggttg
    13861 attgaataag atgtgttttt caatgctgcc attaaaacac agcaaaatta ggtttgaatt
    13921 agcaggaagt taagaagcgt aaatgccgtc tgaaaaaaac agagccgtca aacggctccg
    13981 ttttccttat gcttctttgg tttcagccgc cttttgacgc aggcgcaagc tcaattcgcg
    14041 caactgcttg tcgtccacgc tgttgggcgc gttggtcagc aggcattggg cacgttgtgt
    14101 tttcgggaag gcaatcacgt cgcggataga ttcggcgccg gtcatcaggg taaccaaacg
    14161 gtcgaggccg aatgcaaggc cgccgtgagg aggcgcaccg aatttcaggt tgtccaagag
    14221 gaagccgaat ttctcttgtt gctcttcagg gctgattttc agcgcggcaa acactttctc
    14281 ttgtacgtct gcacggtgga tacggataga gccgccgccg atttcccagc cgttcaatac
    14341 catgtcgtag gcacgggcca ggcagtttgc agggtcggaa accatcaggt cttcatgacc
    14401 ttctttaggc gcggtaaacg gatggtgtac ggcaacgtag cggtcggctt cttcgtcgta
    14461 ttcgaacatt gggaaatcga caacccacaa aggtttccat tcgtctgtga agtagccgtt
    14521 gtctttgccg tgttccaagc cgactttgat acgcaatgcg ccgatggctt cgttcacaac
    14581 tttggttttg tccgcaccga agaagataat atcgccgttt tgtgcgccgg tacgctcgat
    14641 gatttctttc agggcattct cagacaggaa ttttacgatt ggagattgca ggccgctgtc
    14701 ttcgccgttg gaaaggttgc ccacatcgtt tactttgata tatgccagac ctttcgcgcc
    14761 gtagataccg acaaatttgg tgtattcgtc gatttctttg cggctgaatt tcgcaccgtt
    14821 cggcacgcgc agggcgacta cgcggccgcc tttcatatct gctgcgccac ggaagacttt
    14881 aaactcttcc gtcttcatca ggtcggtcaa ctcggtaaat ttcaagccga tacgcatatc
    14941 cggtttgtca gagccgtaat agaacatcgc ttcggcgtaa ggcatacgcg gaaaaccgcc
    15001 caagtccacg cccaacgcgt ctttgaagac ttgtttcgcc ataccttcgg tgatgtccat
    15061 aatttcatcc tcgttcaaaa acgaggtttc caagtcgatt tgcgtaaatt cgggctggcg
    15121 gtcggcacgc aggtcttcgt cgcggaagca cttggtgatt tggtagtaac ggtcgaaacc
    15181 cgccaccatc aacagttgtt tgaataattg cggcgattgc ggtagcgcga aaaactcgcc
    15241 cggatgaacg cggctcggca cgaggtagtc gcgcgcgcct tcaggcgtgg agcgggtcag
    15301 catcggggtt tcaatgtcga tgaaaccttg cgcgtccaag tagcggcgaa cgcccatagc
    15361 cacttggtaa cgcaggcgca ggttgcgctg catcaccgga cggcgcaagt cgataacgcg
    15421 gttggtcagg cgcacgtttt cgctgatgtt ttcatcgtcg atttggaacg gcggcgtggc
    15481 ggcagcgttc aagacttcga tttctttggc aagaatctcg attttgccgg aaatcatttt
    15541 gtcgttggtc gtgccttcgg gacggttgcg tacgcggccg gtaatgctca aaacgtattc
    15601 gttgcgagcg gaatcggcag cggcaaacgc ttcgggcgtg tcggggtcaa tcacgacttg
    15661 gacgatgcct tcgcggtcgc gcaggtcgat aaaaatcaca ccgccgtggt cgcgccgacg
    15721 gtgtacccag cctttgacgg taacggtttg gtctaagtat tgctcgctaa tcagaccgca
    15781 atagttggta cgcataaaat caccttttat tgatttaaac tgaaaacaga aaatgccgtc
    15841 tgaacggcgg ctttattgtt gttcgggcaa atccgccttt tcagacggca taggtcctgc
    15901 caatgttttg acgggcaggt cgtcagggat gaccataccc agcgaaatca catatttcaa
    15961 cgcttcgtcc acgctcatat cgagttcgcg cacatcgctt ttctttacca taatatagta
    16021 accgccggtc gggttgggcg tggtcgggac atacacggaa agataatcgc catcctgcgg
    16081 caatgcggcc ttaaccgcat tcgacacctg ccctgacacg aaagcaatcg tccaaatacc
    16141 cgattgggga aacggcacga gtaccggcgt tttaaacgag cggctgctgt cggacagcag
    16201 cgattcggat acttttttca cactcgagta gatggatttg acaaccggaa tccgccccaa
    16261 caggctgtcc cacgcggcaa gaatctgccg gcccaatacg ttggcggcaa ataatccggt
    16321 tacaaacaat acggcaatgg caacaataac gcccagcccc gggatattga agcccaaaac
    16381 atattgcggc cgccattgct tcggcagcag gttgacgagc tgatcggacg cggaaacgat
    16441 ataggaaacc acccaaaccg ttaccgcaat cggcagccag accaaaatgc ccgtaatcag
    16501 atattttttt aacgccttgg cagctttgcc gccttcggcc gcaggttccg tcatcttgct
    16561 tgattccgac aaagtccgta caaaccgcac attatacgcg tttgcccgga ttcaaacgaa
    16621 atttttatcc cgccccgcca aaccgccggc ggcttcagac ggcacggcaa cttgatatat
    16681 cgtctgaaca cgcggttcag atgccgtcca agtcgttgaa catcaacccg ataccgatac
    16741 cgttctgctt gtggttgtag tcgatcaggc tctcgccgta accgtggaat ccgcgtacca
    16801 cgcctttgag tttgccctta atcggaaacg tgtaggcggc ttcaatcgcg ccgtagcccg
    16861 ttttggggtt gtagcgcaat acggaataca cattctgcct gtcgttcagg cggtactgca
    16921 gcttcacgtc gccatacccc atatagtcgg caatatcggg attgtcgttt ttatcgccgc
    16981 tctgatcgaa cgcacgcacc cacacgcgcg gaatcaccgt caatttgccc cattccatgc
    17041 ctgccatggc gtaaatcctg ttccacgaac gcgattcggg acggctctgt ccgttggact
    17101 ggtggacaaa acccgcaccg agcatacgca gcctgccgcc gaacggcaaa tctgccttca
    17161 caggctgggt caggaaaatt tcaggtttgt aatccgtatt gcggaacggc gcggatttcc
    17221 tgccttggtt gtaaatctgc caatcggatc tttgggtgta gccgaaccac agatccgcgc
    17281 gggttttaaa caaatcttcg gcaattttgc ttttgaacga aacctgcaat ttggtttccg
    17341 cacgtttctg ctgtccgaat ttttcctgta cagtcgtacc gcgcgtcggc gaacccgggg
    17401 catagttggg cgaattgttg taccagagcg gcataaggta catcggattg tgttcgcgta
    17461 cgcccaacag cccgcgcaaa tcgtttttgt ccaagtcgta catcaggctc aaaggcgtat
    17521 agatgtcggc ggtttcgccc gcactgtcgg caggaagcgc atccccgcct ttttcaacaa
    17581 caatgaccgc ctcgccctta tccaggctgc tgcggacggt ttccgtcaga ttgagtacgg
    17641 ctttcgactc ctgcccttcc tgccctgccg aagacggaag ctgtgccgca aaaatcctgt
    17701 cgtaacacgc caaacgcgta acattgtccg tcaaagcggc gcattgcagc gcggtctctc
    17761 caaaagcgga tgccatcggc aacagtcctg tcaaaagaat atagcgcata ttccgtgtat
    17821 tcatctccgc ccccattgtc ggcatattgg ttttcaaacg gcattttata gcggattcgg
    17881 ataaaaaccg caccctttcc gccatttcgg gattttgccc ccgcaataca gaaaaccccg
    17941 aaaccgtcgg gcttcagggt tttccgctta tcgcgtatca accgccttgg cggttttgca
    18001 aaaatcaagc cagtgctttt acttttgcag acagacggct tttgtggcgt gccgctttgt
    18061 ttttgtggaa cacgcccttg tcggcgatgc ggtcgatgac tttgacggac tcttggtaaa
    18121 ccgcttgtgc ggcagcttta tcgcctgctt cgactgcttt caatactttt ttcactgcag
    18181 tgcggaatgc ggtacgcagg ctagcgttgt gggcgcgttg tttgacggac tggcgggcac
    18241 gtttgcgtgc ttgtgcgctg tttgccatat tggaatctcc tgaaagaaaa ataaattctg
    18301 taaacgcgca attttaaagg gggtttacat tatatgcaag ccttttctct ctatccgccc
    18361 gtcggacacc ctgacagcgc aaaaaaacgg caaaatcgtg cggacgtttt ccccctcttt
    18421 tgaaaacagg gtaatttttt agggaaatca gtccaatatc actttgagtt tgcacaaatt
    18481 tactttacaa tgcccaaacc ttgcgccggg gtaacgatgg ggcgcggggt tttctttttt
    18541 tccaacctct ttcatcagga atctgcccgt atgaaaaaat ccgtattagc cgttttggcc
    18601 gcgctgtctc tggcagcctg cggcggcagc gaaaaaaacg ccgtgcaacc tcaagcagac
    18661 gctgcgtctg ctgccaatgc cgaagccgct gccaccgata ctctgaacat ctacaactgg
    18721 tcgaactatg ttgacgagag tacggtcgaa gactttaaaa aagccaacaa tctgaagctg
    18781 acttacgact tgtacgaaaa caacgaaacg ctggaagcca aaatgctgac cggcaaatcc
    18841 ggttacgatt tggttgtgcc gggcatcgcc ttcctgccgc gccaaatcga ggcgggcgcg
    18901 tatcaaaaag tcaacaaaga cctgattccc aactataaaa acatcgatcc cgaactcttg
    18961 aaaatgctgg aaaccgccga cccgggcaac cagtatgccg tcccctattt ctccggcgtg
    19021 aacacgattg cgattacggc gaagggcaaa gagcttttgg gcggcaagct gcccgaaaac
    19081 ggctgggatt tgctgttcaa acccgaatac acccacaagc tgaaatcctg cggcatcgcc
    19141 ctgtgggaca ccccgagcga aatgttcccg attttgctga actacttggg caaagacccc
    19201 aaaggttcga atcctgaaga tttgaaggcg gcggcggaag tgttgaagtc tatccgtccg
    19261 gatgtcaaac gtttcagccc gtccatcatc gacgagctgg cgcgcggcga catctgcctg
    19321 gcggcaggca acggcggcga tttgaacttg gcgaaagcac gttccgaaga agtgaaaaac
    19381 aacgtcggca tcgaagtgct gaatccgaaa ggcatgggct tctggattga gtcttggctg
    19441 attcccgccg atgcgaaaaa cgtcgccaat gcccacaaat acatcaacta cacgctcgac
    19501 cccgaaatcg cggcgaaaaa cggcatcgcc gtaacctttg cccccgccag caaaccggcg
    19561 cgcgaaaaaa tgcctgccga gctggtgaac acccgttcca tcttcccgaa cgagcaggat
    19621 atgaaagacg gtttcgtgat gccgcaaatg agtacggatg cgaaaaaact gtcggtcagc
    19681 ctgtggcaga aaatcaaagt cggcaccaac tgatttgaag cattaaaaat gccgtccgaa
    19741 catcgttcgg acggcatttt atattggatt gaaatagaaa tatttatata gtggattaac
    19801 aaaaatcaga acaaggcgac aaagccgcag acagtacaaa tagtacgaaa ccgattcact
    19861 tggtgcttca gcaccttaga gaatcgttct ctttgagcta aagcgaggca acgccgtact
    19921 ggtttttgtt aatccactat accgtcgttc ccacggtcag gatttcagat tgcggacatc
    19981 tgtcagaaaa gacaaaaaac cttccgccgt cattccctac aggcgagaat ccgatccgtt
    20041 gaaattcggt tgttttaaat aaattcttgc agctttgatt ttctgttttt ccgataacgc
    20101 cgtaactttg aaacgcgaaa gcggtaatcc gatccgttgg gattttgcaa cttcaaatca
    20161 atccgcaaac tgaaatcccg tcattcccgc gcagtcgtga atccgaacgc gtccgcacga
    20221 aaacctgcat cccgtcattc ccacggaagt gggaatccag ttttttgagt ttcagtcatt
    20281 cccgataaat tgcctta
//
LOCUS       DAXHKX010000019        19598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHKX010000019 DAXHKX010000000
VERSION     DAXHKX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.019"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(153..353)
                     /locus_tag="ACFMWZ_000589"
     CDS             complement(153..353)
                     /locus_tag="ACFMWZ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934587.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(367..921)
                     /locus_tag="ACFMWZ_000590"
     CDS             complement(367..921)
                     /locus_tag="ACFMWZ_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934588.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(1006..1506)
                     /locus_tag="ACFMWZ_000591"
     CDS             complement(1006..1506)
                     /locus_tag="ACFMWZ_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934589.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHPLPL"
     gene            1910..3106
                     /locus_tag="ACFMWZ_000592"
     CDS             1910..3106
                     /locus_tag="ACFMWZ_000592"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC5934590.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            complement(3209..4453)
                     /gene="clpX"
                     /locus_tag="ACFMWZ_000593"
     CDS             complement(3209..4453)
                     /gene="clpX"
                     /locus_tag="ACFMWZ_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC5934591.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPPESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            4644..5015
                     /gene="rbfA"
                     /locus_tag="ACFMWZ_000594"
     CDS             4644..5015
                     /gene="rbfA"
                     /locus_tag="ACFMWZ_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC5934592.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            5048..5980
                     /gene="truB"
                     /locus_tag="ACFMWZ_000595"
     CDS             5048..5980
                     /gene="truB"
                     /locus_tag="ACFMWZ_000595"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC5934593.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYAIDI
                     AEFDSPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFEENLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAVKNTGCV"
     gene            6270..6749
                     /locus_tag="ACFMWZ_000596"
     CDS             6270..6749
                     /locus_tag="ACFMWZ_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC5934594.1"
                     /translation="MKADIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPAI
                     IGLLLGNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSSEEGEEIYFKHSNSQPASQPASQPASQPASQPIIH"
     gene            6679..8445
                     /locus_tag="ACFMWZ_000597"
     CDS             6679..8445
                     /locus_tag="ACFMWZ_000597"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC5934595.1"
                     /translation="MASQPASQPASQPASQPASQLYTKLTRKRQEIFFNQTLAFDEID
                     RLFDAKAFSKFSRHTADGKQAVGEIKRHSDGTPAENLIIKGNNLIALHSLATQFKGKV
                     KLIYIDPPYYFVKKKQQDSFGYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDD
                     DGNAYLKILLDEIFGFENFIGNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFY
                     EFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITD
                     EEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQ
                     TDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFK
                     NPKPEELIQDFITITTNESDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVE
                     RLKKVIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLC
                     ERYFLKYNVSVNEFSQIIQEPEFQSLALDEQKQMVLEMLDLNQMYVSLSEMDDEQFAG
                     CLNDDDKALSRAFYQTEKKDGE"
     gene            8435..11173
                     /locus_tag="ACFMWZ_000598"
     CDS             8435..11173
                     /locus_tag="ACFMWZ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC5934596.1"
                     /translation="MANNKMLFEVIENRKAVYLEDGDDEKCRLPEFVERNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIGSTHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAPTKSKKRDAEIEHKGWEH
                     MVLELLLNKNGNPSENVLLEFTATPPEKADVQQKYADKIITKFGLKEFLQKGYTKEIN
                     LVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTVDESKADYLAFLNW
                     VENVQVDDFSFLKTFSTSLNDSDNANEQGKTRTEQALKFMQDNKFGFAHLANWVKQNY
                     QKHNIIITNSENNKTKTEKTDSETEKLLNNLEAADNPIRAIFTVGRLTEGWDVLNLFD
                     IVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFAFEGKQPNKRKFDNDMQH
                     ELRILEELFYYAHDEQSRYISELKNELRKDGYLPEKDDDKVLATFKLKSEFAANKDFR
                     ELLIWANKKIPNPNAKSNNADSLKANPQTLSFQVHGNQLLQETQFTADENDETARQIG
                     TQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLDIQNRNELQNNLLKD
                     WQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGTKEFTPKKLWEIFGT
                     PKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARLGDLKSKYDVHLIRN
                     EEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFIEPKGEHLVETDGWK
                     EEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTESFPLGAASLEKMK"
     gene            complement(11317..12489)
                     /locus_tag="ACFMWZ_000599"
     CDS             complement(11317..12489)
                     /locus_tag="ACFMWZ_000599"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC5934597.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            12839..13285
                     /gene="iscR"
                     /locus_tag="ACFMWZ_000600"
     CDS             12839..13285
                     /gene="iscR"
                     /locus_tag="ACFMWZ_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC5934598.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNSDGSRVVQFTHIH"
     gene            13314..14528
                     /locus_tag="ACFMWZ_000601"
     CDS             13314..14528
                     /locus_tag="ACFMWZ_000601"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC5934599.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLGHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            14594..14722
                     /locus_tag="ACFMWZ_000602"
     CDS             14594..14722
                     /locus_tag="ACFMWZ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934600.1"
                     /translation="MNEQDLDLDNLDNLLEDFDGVTVEGGVDSENDDGCEGGACKI"
     gene            14788..15174
                     /gene="iscU"
                     /locus_tag="ACFMWZ_000603"
     CDS             14788..15174
                     /gene="iscU"
                     /locus_tag="ACFMWZ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC5934601.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            15261..15581
                     /gene="iscA"
                     /locus_tag="ACFMWZ_000604"
     CDS             15261..15581
                     /gene="iscA"
                     /locus_tag="ACFMWZ_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC5934602.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(15541..15762)
                     /locus_tag="ACFMWZ_000605"
     CDS             complement(15541..15762)
                     /locus_tag="ACFMWZ_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC5934603.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            15844..16344
                     /gene="hscB"
                     /locus_tag="ACFMWZ_000606"
     CDS             15844..16344
                     /gene="hscB"
                     /locus_tag="ACFMWZ_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC5934604.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            16442..19192
                     /gene="gyrA"
                     /locus_tag="ACFMWZ_000607"
     CDS             16442..19192
                     /gene="gyrA"
                     /locus_tag="ACFMWZ_000607"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC5934605.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIRLIKESPNAVEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            19349..19598
                     /locus_tag="ACFMWZ_000608"
                     /pseudo
     CDS             19349..19598
                     /locus_tag="ACFMWZ_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         5486 a         4972 c         4578 g         4562 t
ORIGIN      
        1 cttttccgct ttggcaacga ttgaaaattt ccgtaaattc aaatatctag attccttcct
       61 gcacgggaat gacacggaag ggtttcagat gcagggtggg cattcctgcc caccaaatcc
      121 cgcccttgca acggtgggca agaatgctcg ccctacggct tgactgttcg atatgatgcc
      181 gtctgaaaac ccaacggcgg catgacaatg ccgccctgcc aacgcacgta aatcagaatt
      241 gccatcccga catcaaacgc ttggaaacaa aatgccgtct gaaaatcaaa cggcaacata
      301 acaatgtccc taacaaatgc aaaaatgccg tctgaaagct cttcagacgg cattggtgcg
      361 ccgggtttac cgcctcctgc cgaaaccgcg catagcgggg cggcggtaat tggcgggcgg
      421 gcgcgttgtc gggcggtaac gctgcgcctg cgccgcctgt tgttttgcac ggaggctgcg
      481 cgtgttcaaa tccctgctgg tgcgcgcatt ggggcgtgcg gactgatggt aggctgcacg
      541 cgcgcgccgg gcgacgggac tgtcttggtt gcctgcccgt gtgaatttgt ttgccagcgc
      601 gttgccgata aacgcgcctg ccgccgcgcc gaccaggctt tgcagcagcc agcttcctgt
      661 cgattggtcg taaatatact gctgcccgtc tttaccggta acgggttgcc cgttgttgcc
      721 gtttgcctgt gcttcggcag gaatggtgtc tttgactgct tcgggagtca gttggtaaac
      781 cgtatcgtct gcctgctgtg cgagctgctg ttgcagggct tcaatctgtt tctgctgctg
      841 ttcgagccgc gcctgcgtgt cgtcttggca ggcggcgagt gcgaatgttg cgataagcgc
      901 ggaggcgatg atttttttca tgtgtgtcct gtttgggtgg aaaatcggtt ttattgtatc
      961 gccgtcggga attttggcaa gcattctgcc ggcaaatcgt gatgtttaca ggggcagggg
     1021 gtgcaatttg cggacaaatg cgaggctgtt ggcgactggg ttgcctttgt tttcgacttc
     1081 gtgttcggtt tctacggtca gcaggcggcg gtttttgtgt ttgttcaggc tcaattcggc
     1141 ttgtgcgggt gtgaaaaaca ggcggtgttt ttcggcaaag cggcgggcgc ggctgttggc
     1201 ggttttcaaa atctgaagca gcgatacgtc cagatggaag cgtcgcacgc ccaatacgag
     1261 aatccgtgcg gcaaaaaaat cggcggcatc ggtaaactcg gcggggctgc tgcggctgaa
     1321 gtcgtgccgt tcggcggcta tcagggcggc aacggtgcgg atttgcggaa tcatcgattc
     1381 gctgataagt gtgtcgtccc gccctgcatc gagaagcatg ggggaaaaat cctgtgcatc
     1441 atcgacaata atgctacaag tgtgcagggt ttcgttttgt gcggcggttt ggggcattgc
     1501 attcatggtc attttcctga ttctgtcgtg tgttgccgaa tcgggcgacc tgtgtgaagg
     1561 taacaaaaaa gccgccccgt tttcgagcgg cctgttttgc gtatgggatg gatttcaagc
     1621 aagcgcaaaa aagtaccgca cgtctgtgtg gtaccaatag caataagcgg ttgtaaattt
     1681 tttgccttgc atgatgaaat gccgtctgaa gataaaaata ttggggagat tctaaatcaa
     1741 aacgctgccg cgcctcaagc attttatcga aatttttttg atttttcatc tatccgattg
     1801 aaaatatttc ggtttatttt taccgctgcc cgatattgtc ggcaatttcc ctttatctgc
     1861 tttgaaaaac ggtgcataat cccgagcaaa accgcaatca ggagcaatta tgcaaaacta
     1921 tctgaccccc aatttcgcct ttgccccgat gattcccgaa cgcgcttcag gcagccgcgt
     1981 ttgggatacg aaagggcgtg aatatattga tttttcaggc ggtatcgccg tcaatgcgct
     2041 gggacactgc caccctgccc ttgtcgatgc tttaaacgcg cagatgcaca agctgtggca
     2101 catttccaat atctatacga cgcgtccagc gcaggaattg gcgcaaaaat tggttgcaaa
     2161 cagttttgcc gacaaggttt ttttctgcaa ctcgggctcg gaagcgaatg aggcggcgtt
     2221 gaagctggcg aggaaatacg cccgcgaccg tttcggcgga ggaaaaagcg aaatcgtcgc
     2281 ctgtatcaac agttttcacg gacgcacgct gtttaccgtg tccgtcggcg gtcagccgaa
     2341 atacagcaag gattatgcac ccctgccgca aggcattacg cacgttccgt tcaacgatat
     2401 tgccgcgctg gaagctgccg tcggcgaaca gacctgcgcg gtcatcatcg agccgataca
     2461 gggcgaaagc ggcatcctgc ccgccactgc ggaatatttg caaaccgcgc gccgtctgtg
     2521 cgaccggcac aatgcgttgt tgattttgga cgaagttcaa accgggatgg ggcatacggg
     2581 caggctgttt gcctatgaac attacggcgt tgttcccgat attttgagtt cggcaaaagc
     2641 cttgggctgc ggctttccga tcggcgcgat gctggcgaca gaaacgattg ccgccgcctt
     2701 ccaaccgggc acgcatggct cgactttcgg cggcaacccg atggcgtgtg cggtcggcag
     2761 ccgcgccttc gacatcatca atacgcccga aactttaaac catgtccgtg aacaggggca
     2821 gaaacttcag acggcattgc tggatttggg caggaaaaca ggcttgttct cacaggttcg
     2881 cggcatggga ctgctgctcg gctgcgtgtt ggacgaagcc tatcgcggac gcgcatccga
     2941 aatcaccgcc gccgccttga aacacggcgt gatgattttg gttgcgggtg cggacgtatt
     3001 gcgtttcgcg ccttcgctgc tgttgaacga tgaagatatg gcggaaggtt tgcggcgttt
     3061 ggaacacgcg ctgacggaat ttgccgcgac atcagacaat ccgtaaaact caaatgccgt
     3121 ctgaaggcgg gaaggcttca gacggcatca gaaacaaaaa accgcttcgg aaacgtggtt
     3181 caacgttccg aagcggtttt gtttgccatc aggactcgaa caccaattcc ggttccctgc
     3241 cctcttcgat gactgcctta ccgaccacca cttttttcaa gcctttcaaa tcaggcaggc
     3301 ggtacatggt atcgagcagg cagcgttcga cgatggaacg caggccgcgc gcgccggttt
     3361 tgcgctccat tgcctgacgc gcgatggaac gcaatgcgcc ttcttcaaat tccaactcga
     3421 cattttccat accgaacaag gcttgatact gcttgaccaa agcatttttc ggctcggtca
     3481 aaatattaat cagcgcgtcc tcatccagtt cttctagtgt tgcaatcacg ggcaaacgtc
     3541 cgattaattc cggaatcaga ccgaatttaa tcaaatcttc cggttcgacg atgccgaaca
     3601 gcttggtaat gtcggcattt tcgtccttgc tgtgaacaga cgcaccgaaa ccgataccgc
     3661 ctttttcagt acgctggcgg attacttttt ccaagcctgc aaacgcgccg ccgcagataa
     3721 acaggatgtt ggtggtatcg acgttgataa attcctgatt cggatgcttg cggccgcctt
     3781 gtggcggaac acttgccacc gtaccttcaa tcagtttcag caaggcttgc tgcacgcctt
     3841 cgccggacac gtcgcgcgtg atggacggat tgtcgctttt gcgtgaaatc ttatcgattt
     3901 cgtcgatata gacgatgccg cgttgggctt tttcgacatc gaaatcacat ttgcccaaga
     3961 gtttggtgat gatttgctcg acatcttcgc cgacataacc tgcttcggtc agcgtggttg
     4021 catctgccat cacgaacggc acatccagtt tgcgcgccaa agattgcgcc agcagggttt
     4081 tacccgatcc ggtcgggccg ataagcagga tgttggattt cgacaattcg acattagctc
     4141 cggctttcgg gtggcgcagg cgtttgtaat ggttgtacac cgacaccgcc aaggctttct
     4201 tggcttgttc ctgaccgata acgtggtcgt tgaggttggc gacgatttcg gcgggcgtgg
     4261 gcagcttgcc ggattcttcc ggctcccctc cggcactttc cggaggcgtg ccgtcatttc
     4321 cgtcttcatg caatatttca atacagtttg agacgcattc gtcgcagata aaggcgtttt
     4381 cgccctcaat caaatgtttg acgtgtgatt tggattttcc gcaaaaggaa caggtacggt
     4441 tttcgttgga catggctttc tttacaatgt atgcgttaca gaaaacggca cgtgccgttc
     4501 gggttgccaa gtataataac tatatccgtt cttatcaatg tattacctta aaatcccgcc
     4561 gattaggcta taatacgccc tttcgcaacc gccccggcgg caaaaatgcc gtctgaaacc
     4621 aaatctgaaa tctgaggata ttcatgagaa aaccccaacg cggctatgcc cgccaagacc
     4681 gtgtcaaaga acaaattatg cgcgagcttg ccgaactcgt ccgtaccgga ctgaaagacc
     4741 cgcgcgccgg cttcattacc gtcaacgaag tcgaagttac ccgcgattac agccacgcca
     4801 ccgtgttcta caccatttta aaccaagacg cgcgcgaaat tacggaagaa gtgctggaac
     4861 acgcgcgcgg acacctccgc agcgaattgg ccaaacgcat caagctgttc aaaatccccg
     4921 aactgcattt caaatacgac gaatccttgg aacgcggttt gaacctgtcc gcccttatcg
     4981 accaagtagc ggcggaaaaa ccggttgaag actgacggat atgcccatgc cgtccgaaca
     5041 tcgaaccatg aatacaggca aaccccaaaa acgtgccgtc aacggtgttt tgctcttgga
     5101 caaacccgaa ggcctttcca gcaacaccgc gctgcaaaaa gcgcggcgtt tgttccgtgc
     5161 cgaaaaagcc ggacataccg gcgtgctcga ccctttggca accgggcttt tgcccgtctg
     5221 cttcggcgaa gcgaccaagt tcgcccaata cctgctggat gccgacaaag cctacaccgc
     5281 cacgctgaaa ctcggcgaag ccagcagcac gggtgatgcc gaaggcgaaa tcatcgctgc
     5341 cgcacgcgcc gatatttcct tagccgaatt tcagacggcc tgccaagccc tgacaggcaa
     5401 catccgccaa gtgccgccga tgttttccgc cctcaagcac gaaggcaaac cgctgtacga
     5461 atacgcccgc aaaggcatcg tcatcgaacg caagccgcgc gatattaccg tttacgccat
     5521 cgatattgcc gagtttgact cacccaaagc cgtcatcgac gtgcgttgca gcaaaggcac
     5581 ctatatccgc accctcagcg aagacatcgc caaacacatc ggcacgttcg cccacctgac
     5641 cgccctgcgc cgcaccgaaa ccgccggctt taccatcgcc caaagccaca cgctcgaagc
     5701 cttggcaaat ttgaacgaaa cagaacgcga cagcttgctg ctgccctgcg acgtattggt
     5761 tgcccatttt ccccaaaccg ttttaaacga ttatgccgtc catatgctcc aatgcgggca
     5821 acggccgcgt ttcgaggaaa acctgccttc cgatacgccg gtacgcgttt acacggaaaa
     5881 cggccgcttt gtcggactgg cggaatatca aaaagaaata tgccgtctga aagccttgcg
     5941 cctgatgaac acggcggtta aaaatacggg ctgtgtttga ataatgtgtt gatatagtgg
     6001 attaacaaaa accagtacag cgttgcctcg ccttgccgta ctatttgtac tgtccgcggc
     6061 ttcgtcgcct tgtcctgatt tttgttaatc cactatattt ccgcaaaatc acgacacact
     6121 cggacacccg ccccgcttat tgcaactttg cgaacgcccc cggaaacagc aaagataaca
     6181 aatgattgat tttattagta tttatttgca aaaccatttg ccgttacaca agaatggcac
     6241 attaaaatga ctgatgagga tttataacga tgaaggcaga cattcaaacc gaattaaccc
     6301 aagccctgct atcacacgaa aaagtatggg cgaacgaaga aaaaaccatt ttagccaaaa
     6361 acattctgtt ggatttggtg gaaaaaaccg acccggccat tatcggtttg ttattgggga
     6421 atgatgagtt aaaacgccat ttttttgtgg aagtgaatgg tgttttggtg tttaaattgc
     6481 aggattttcg ttttttcttg gacaaacaca gcgtcaataa ttcctacaca aaatacgcca
     6541 accgcattgg tttgacggac ggcaaccgct ttttgaaaga cagttcggat attgtgttgg
     6601 attttccgtt taaagattgc gtattgaatg gcgggcaaag cagcgaagaa ggcgaagaga
     6661 tttattttaa acacagtaat agccagccag ccagccagcc agccagccag ccagccagcc
     6721 agccagccag ccagccaatt atacactaaa ttaacccgaa aaagacaaga aatctttttt
     6781 aatcaaacgc ttgcttttga tgaaattgac cggctttttg acgcaaaagc attctcaaaa
     6841 ttctctcgcc acaccgcaga cggcaaacaa gcggttggcg aaatcaaacg acattcagac
     6901 ggcacaccag ccgaaaatct cattatcaaa ggcaataatc tgattgccct gcattcgctt
     6961 gccacgcagt ttaaaggtaa agtgaagctg atttatattg atccgcctta ttattttgtt
     7021 aaaaagaaac aacaagatag ttttggttat aacactaact ttaaattatc tacttggctt
     7081 acatttatga aaaatcgttt atcaattgcc aaagaattat taactgatga tgggattatt
     7141 gttatttcca ttgatgatga cggaaatgct tatttaaaga ttttgttaga tgaaattttt
     7201 ggatttgaaa attttatcgg aaatttaccc actattatga atttaaaagg taataatgat
     7261 gaatatgctt ttgcaggcac tcacgaatac acactagtct ttgctaaaaa taaagataaa
     7321 tctacttttt atgaatttcc aatagatgaa gataattttt tagagaaatg ggaagaagat
     7381 gaaattgggt tttataaaaa aggagctcca atgcgagcaa caggaaccga agataaaaga
     7441 gaagatagac ctgaaatgtt ctatcctttt ttggtaaaaa ataacacagt tagtacaatt
     7501 acagatgaag aattttctca aatctataat aaagatttag aagtattcaa tgatgatttt
     7561 attcaaaaac taaaagagaa atacgaaaat cttggataca attttatttt acctattgct
     7621 gataaacaat gggggcgttg gcgttggggg tattcaataa agaataaggc aaggctgcag
     7681 acggatatta ttgttagcca atctaaaaat ggaatttcct tgtataaaaa acaaagacct
     7741 gaattagatg atttacctac aaaaaaacct aaaactattt tttataaacc tgaatacagt
     7801 agtggtaatg gaactgaaca gatgaaaaat ctttttggtg aaaaggcatt taaaaatcct
     7861 aaaccagaag aacttatcca agattttatt accatcacaa ccaacgaatc cgacatcgtc
     7921 ttagactacc atcttggtag tggcacaacc gccgccgttg cccacaaaat gaatcgccaa
     7981 tacatcggta ttgaacaaat ggattatatt gaaacgcttg ccgttgaacg cttgaaaaaa
     8041 gtgattgatg gcgagcaagg cggtatttcc aaagccgtga attggcaagg tggtggcgag
     8101 tttgtttatg ccgaacttgc cccatttaac gaaaccgcaa aacaacaaat tttggcttgc
     8161 gaagattcag acggcatcaa aacgctgttt gaaggtttat gcgaacgcta tttcttgaaa
     8221 tacaacgtca gcgtaaatga atttagtcaa atcattcaag agcctgaatt tcaatccttg
     8281 gcattagacg aacaaaaaca aatggtgctt gaaatgttgg atttaaatca aatgtatgtt
     8341 tcattatccg aaatggatga tgagcaattt gcaggttgct taaatgatga cgataaagcg
     8401 ttaagccgtg cgttctatca aacggagaaa aaagatggcg aataataaaa tgttgtttga
     8461 agtgattgaa aaccgcaaag ctgtgtattt ggaagacggc gatgatgaaa aatgcaggct
     8521 gcctgaattt gtagaacgca atttaaaata tccgtttttt gaatggcaaa aatcggcttt
     8581 agaaaacttt gtgatttttg accgcacttc aaaattaaag gattttcccg atatcaaaaa
     8641 ccgccctacc catttgctgt tcaatatggc aacaggtgca ggcaaaacga tgatgatggc
     8701 ggcgttgatt ttgtattatt ttgaaaaggg ttatcggcat tttctgtttt tcgtcaatca
     8761 aaacaatatc gtagataaaa cggaaaataa ttttattggc tcaacgcacg caaaattttt
     8821 atttaccgag aagattttgc aaggtgatac ggtaattcct attcgcaaag tggaaatgtt
     8881 tagcccacat tcagacggca ttgaaattaa atttaccagc attcaaaaac tatataacga
     8941 tattcacacc gaacgggaaa accaaaccac attggcggat ttgcataaat tgaaccttgt
     9001 gatgctgggc gatgaagcgc accatttaaa cgccccaact aaaagcaaaa aacgcgatgc
     9061 cgaaattgaa cacaaaggct gggagcatat ggttttggaa ttgttactca ataaaaatgg
     9121 caatcccagc gaaaatgtgc tgttggaatt taccgccacg ccacctgaaa aagctgacgt
     9181 gcaacaaaaa tacgctgata aaatcatcac aaaatttggt ttaaaagaat ttttgcaaaa
     9241 aggttatacc aaagaaatca atttggtatc cagtacactg agcaagaaag agcgggtgtt
     9301 gcacgcttta ttgtttgctt ggtatcgcca tcaaatcgct ttgaaatatg gcattgccaa
     9361 tttcaaacct gtgatgttgt ttagaagtaa gacggttgat gaatcaaaag cggattattt
     9421 ggcattttta aattgggtag aaaatgtgca agtagatgat ttttcgtttt taaagacatt
     9481 ttcaacaagt ttaaacgata gcgataacgc caacgaacaa ggcaaaaccc gcaccgaaca
     9541 agccctaaaa tttatgcagg acaataaatt tgggtttgca catttagcaa attgggtaaa
     9601 acaaaattat caaaaacaca atatcattat taccaactcc gaaaacaaca aaaccaaaac
     9661 cgaaaaaacc gacagcgaaa cggaaaaatt gctaaacaat ctggaagcgg ctgataatcc
     9721 aatccgtgcc atttttacgg tgggtagatt aaccgaaggt tgggacgttt taaatttgtt
     9781 tgatattgtg cgtttgtatg aagggcaaaa cggcggcggt tcaaataaaa aatcatgcaa
     9841 aacggcaaat acaacggtat cagaaaaaca gttgattggt cgaggcgtgc gttattttcc
     9901 atttgcgttt gaaggtaaac agccgaataa acgcaaattt gacaacgaca tgcaacacga
     9961 attgcgtatc ttggaagaat tgttttatta cgcgcacgat gagcaatccc gctatatttc
    10021 agaactgaaa aatgagttgc gaaaagacgg ttatttgcct gaaaaagacg atgataaggt
    10081 attggcaaca tttaaactca aatctgaatt tgccgctaat aaggatttta gagaattgtt
    10141 aatttgggcg aataaaaaaa tccccaaccc aaacgccaaa agcaataatg cagacagcct
    10201 aaaagccaat ccacaaacgc tttcattcca agttcacggt aatcaactgt tgcaggaaac
    10261 gcaatttaca gccgatgaaa atgatgaaac agcccgacaa atcggcacac aaaataattt
    10321 tactcaaacc ataaaaatga gtgaaatgga gcggcacatt ttcaataaag cattacatat
    10381 caaagggaaa aatagtcaat ctttattcca tttcaaccgt ttgcaaagca agctcgacat
    10441 tcaaaatcgc aatgaattgc aaaataactt gttgaaagac tggcaaattg aatttttagg
    10501 attagggcaa gacaaacagg ttcgcctgga cgataaactt gcaggctgcc tgaaaatctt
    10561 ggaaatggtt gaaaaacatc tgaatgaaag tgatatgccg tttatcggta caaaagaatt
    10621 tacacctaaa aaattatggg aaattttcgg cacgccaaaa caaaaatggg tcaaaaaaga
    10681 tgatgtaaaa acgactattg ccacgcaaaa tgattggtat gtgatgaata attttgccgg
    10741 aacgggtttg gaagaagcgt taattcaatt tatttcagcg cgtttgggcg atttgaaatc
    10801 taaatatgac gttcatttaa tccgtaatga agaagtgttt aaattgaata actttgccga
    10861 tggtgaagga tttatgccgg actttatttt attgctgaaa gataaacaaa aatcttcttc
    10921 caatggtgtg aacgactttt tgcattacca aattttcatt gagcccaaag gtgagcattt
    10981 agtggaaact gatggatgga aagaagaatt tttagaagcc atcactgtgg aatatgggat
    11041 ggataagatc ctgcaaaaag atacaccgca ttatcgtttg atcggtttac cgttttttac
    11101 tgatcatcag aaaaatggac aatttacgga gtcattccct ttaggtgcgg catcgcttga
    11161 aaaaatgaag tagtgcattg caggcaaccc cgtttgacaa aacttccttt acaaaagggc
    11221 gttttgtcag atatttaatc aacacattat taaaatacag ccaaatttta atgccgtccg
    11281 aaccctgtgt tcagacggca tcgtattttt cagtatctaa accgtttccc tgccccaatc
    11341 tttgcctctc aaaatcgaag catcgacatc ttgaatatcg cggtgtcccg taaacgccat
    11401 agatatatcc atttctttat acaggatttc cagcgcacgg gttacgcctt cttcaccata
    11461 cgcacccaaa ccatacagaa acgcccggcc tatcatcgta cctttcgcgc ccaaagccca
    11521 cgccttcaaa atatcctgac cgctgcggat accgctgtcc atccaaactt cgatgtcgct
    11581 gcccactgcg ctgacgatgt cgggcaaggc tttgatggca gacacggtat cgtcgagctg
    11641 tcggccgccg tggttggaaa caatcaatgc gtccgcgccg cttttcgctg ctttttccgc
    11701 gtcttcgggt tccataatgc ctttgataat cagcttgccg ccccacaaat ctttaatacg
    11761 cgccacatcg tcccagctca ggcgcgggtc gaattgttcg gaagtccatg aagacagcga
    11821 agacaaatcg ccgacgttct tcgcgtgtcc gacgatattg cggaacgtgc ggcgttccgt
    11881 gttcagcatt ttcatgcacc attccggttt ggtcgccaga ttgattaaat tggcgatggt
    11941 cggtttcggc ggcgcggaca ggccgttttt gatgtctttg tggcgttgcc ccaaaacctg
    12001 caaatcggcg gtcaaaacca atgccgaaca tttggcatcc ttcgcgcgct taatcaggtt
    12061 ttccataaat tcgcggtcgc gcatcacata aagctgaaac caaaacggcg cgctggtgtt
    12121 ctcggcaacg tcttcaatcg agcagatgga catggtggac aacgtaaacg gaataccgaa
    12181 cttctccgcc gcccgcgccg ccaaaatttc gccgtctgcg tgtgccatgc cggtaaaacc
    12241 cgtcggcgca atcgccaccg gcattttcac atcctgcccg atcattttag tctcaaggct
    12301 gcgtccctcc atattgacca atactttttg acggaagcgg atgtctttga aatccgaagt
    12361 attttcccga taagtcgttt ccgtccacga acccgaatcg atgtaatcgt aaaacatacg
    12421 cggcattttg cgcttggcaa cgcggcgcaa gtcttcgatg caggtcattt tgctcaaatc
    12481 acgtttcatc ttgtttttcc ctgtttgaca gcctgatgga atatgccgtc cgaaaccgct
    12541 tgagccttca gacagcattt caggcaatac ccaaataaat cactaagatt tagataatct
    12601 atatcaatat caaaaataaa tcaaatcgtt ttgaaaccgc ttgccggcaa aataccccga
    12661 ccaccgctcc tcaaaaaaca taaccaactg atttaaaaaa ccaaacaaat aaagtaaaat
    12721 cagactatac cgccatttaa acaaacaatc ggcaacagct cccccttact tgactgaaac
    12781 actcagatat tggacaattc tccccactat aaaaaagccg acacgggcaa ccaccaccat
    12841 gagactgacc accaaagggc gttttgccgt taccgctatg ctggatttgg cgatgaacgc
    12901 gcaaaccggc gccgtcaaac tcagtgccat cagcgaacgc caaaacatat ccctctccta
    12961 tctcgagcaa ttgttcggca aactccgccg cgccggactg gttgaaagcc tgcgcgggcc
    13021 cggcggcggc tacatcctcg ccgcaccggc ggcacgcatc aacatcgccc aaatcatcgc
    13081 cgccgccgaa gaccggctgg acgcgaccca atgcggcagc aaagccaact gccaccacgg
    13141 tgcgccctgc ctgacgcacg acctttggga gaatttaaac aaaaccatca acgactacct
    13201 cggcagcgtt accctgcaaa gcatcatcga acagaaaaac aacagcgacg gcagccgcgt
    13261 cgtccaattt acacacatcc attaaataac acccgaaaaa gaaagagcaa accatgaccg
    13321 tcaaaacccc cgtttacctc gactacgccg ccaccaccac cgttgacaaa cgcgttgccg
    13381 aaaaaatgat tccctatctg accgaaacct tcggcaaccc cgcctccaac agccacgcat
    13441 tcggctggga agcagaagag gccgtcgaaa aagcccgcgc cgacatcgct gccctgatta
    13501 atgccgaccc caaagaaatc gtcttcacca gcggcgcgac cgagtccgac aacctcgcca
    13561 tcaaaggcgc ggcaaacttc tacaaaacca aaggcaaaca cctcattacc gtcaaaaccg
    13621 aacataaagc cgttttagat acaatgcgcg aactcgaacg ccaaggtttt gaagtaacct
    13681 acctcggcgt tcaagaaaac ggtttgattg atttggaaga actcaaagcc gccatccgcg
    13741 acgacaccat cctcgtttcc gtaatgtggg caaacaacga aatcggcgtg gtgcaagaca
    13801 ttcccgccat cggcgaaatc tgccgcgaac gcaaaatcgt cttccacgtc gatgccgccc
    13861 aagcctgcgg caaagtgcct gtcgatgtcg aagccgccaa aatcgacctg ctctcgatgt
    13921 ccgcgcacaa agtgtacggc cccaaaggca tcggcgcgct gtacgtccgc cgcaaacccc
    13981 gcgtccgcct cgaagcccaa atgcacggcg gcggccacga acgcggcttc cgcagcggca
    14041 cattgccgac ccaccaaatc gtcggtatgg gcgaagcctt ccgcattgcc aaagaagaat
    14101 tggcacaaga cactgcgcac tacctgaaac tgcgcgatat cttcctcaaa ggcatcgaag
    14161 gcatcgaaga agtctacatc aacggcgacc tcggacaccg cgccccgaac aacctgaacg
    14221 tcagcttcaa cttcgtcgaa ggcgaaagcc tgattatggc ggtgaaagaa ctcgccgtat
    14281 ccagcggctc cgcctgcacc tccgcctcgc tcgaacccag ctacgtcctg cgcgcgctcg
    14341 gccgcaacga cgaactggcg cactcatccc tgcgtatcac cttcggtcgc atgaccaccg
    14401 aagaagaagt gcaattcgcc gccgaactga ttaaatccaa aatcggcaaa ctgcgcgaac
    14461 tgtcgccgct gtgggaaatg ttcaaagacg ggattgattt gaactcgatt gaatgggcag
    14521 cgcattaaag cgtaccaaca tgccgtccga acctttagac ggcattccaa aaacaaagca
    14581 atcaagagaa aatatgaacg aacaagattt agatttggac aatctcgaca acctgcttga
    14641 agattttgac ggcgttaccg tggaaggcgg cgtcgattcg gaaaacgacg acggctgtga
    14701 aggcggagcg tgcaaaatct aactcaggcc gtctgaaaac cggcaaacaa accacttaaa
    14761 tcatcaaaac attaaggaaa ccacatcatg gcatacagcg ataaagtaat cgaccactac
    14821 gaaaatcccc gcaacgtcgg tactttcgac aaaaacgacg aatctgtcgg caccggcatg
    14881 gtcggcgcac cggcctgcgg cgacgtgatg cgcctgcaaa tcaaagtgaa cgatgaaggc
    14941 atcatcgaag atgcgaaatt caaaacttac ggctgcggtt ccgccatcgc ttcgtccagc
    15001 ctgattaccg agtgggttaa aggcaaaagc ctggatgacg cgctggcaat caaaaacagc
    15061 gaaatcgccg aagagttgga attgccgccg gtaaaaatcc actgctccat cttggctgaa
    15121 gatgcggtaa aagcggccgt tgccgactac cgcaaacgtc aggaaaacag ataaagccct
    15181 tcagacggca tcgtcccgca atgccgtccg aaccgcccgc cgcttcgggt ctgtccgggg
    15241 cgatacaaca aggaagaaat atgattaccc ttaccgagaa tgccgcaaaa cacatcaatg
    15301 actatctcgc caaacgcggc aaaggcttgg gcgtacgctt gggtgtgaaa accagcggct
    15361 gctcggggat ggcgtacaac cttgaatttg tcgatgaagc cgatggcgac gacctgattt
    15421 tcgaaggaca cggcgcacgc atttatatcg acccgaaaag cctggtttat ctggacggca
    15481 cacaagtcga ttacaccaaa gaaggtttgc aggaaggttt caaatttgaa aaccccaatg
    15541 tcaaagactc ctgcggctgc ggcgaaagct tccacgttta aggcataaaa acggcgggac
    15601 agtatcaaaa ccgtcccgcc attttttcgc ttcctgcctg ttgtagctgc ctttgccttt
    15661 ccttttccgt tccaccttgt gccggaacaa atcggatttc actaaggctt ttaaagcatt
    15721 gtcgcgtatt ttgcctttat tgtgctgcac tttgccgccc atattcagtc ctttcgttta
    15781 agaagctgca gattataagg caaaaacagt tttctgccaa aatcttacat ttatcatcct
    15841 actatgtccc aatatttcac cctcttccgg attgaacccg ctttcgatat cgacaccgaa
    15901 aacttggaac aaacctaccg cgccttggcc gcccgtttcc atcccgataa attcgcttca
    15961 gcttccgcct ttgagcaaaa gcaggcagtg atgatgtctt ccaccatcaa cgatgcctac
    16021 cgcaccttga aaaaccccat cgaccgcgcc gcctacctgc tgaaaacatc gggcatcgat
    16081 gccgacgcgc cggagcatac cgctttcgcc cccgaattcc ttatgcagca aatggaatgg
    16141 cgcgaaacgc tgatggaggc acgggcaggc aacgaccttg aatccttgaa aaatctcgac
    16201 aacgaaatcc gcgacgagca agaaaaactg ttctgcggtc tgaaacagtc gtttgcccga
    16261 caagattacg acacagccgc acaacaagtc cgccaaggca ggtttctcga caaactccgc
    16321 aacgaaattt cctcggcatt ataatccgca ccgtgtttca gacggcgtaa ccgccgcacc
    16381 gttccgcgtc aaaatatgct aaaataagca acaatttttt gccatacgaa acattgaaac
    16441 catgaccgac gcaaccatcc gccacgacca caaattcgcc ctcgaaaccc tgccggtaag
    16501 ccttgaagac gaaatgcgca aaagctatct cgactacgcc atgagcgtca ttgtcgggcg
    16561 cgcgctgccg gacgttcgcg acggtctcaa gccggtacac cgccgcgtac tgtacgcgat
    16621 gcacgaattg aaaaacaact ggaatgccgc ctacaaaaaa tcggcgcgca ttgtcggcga
    16681 cgtcatcggt aaataccacc cccacggcga taccgccgta tacgacacca tcgtccgtat
    16741 ggcgcaaaat ttcgctatgc gttatgtgct gatagacgga cagggcaact tcggatcggt
    16801 ggacgggctt gccgccgcag ccatgcgcta caccgaaatc cgcatggcga aaatttccca
    16861 cgaaatgctg gcagacattg aggaagaaac cgtcaatttc ggcccgaact acgacggtag
    16921 cgaacacgag ccgcttgtac tgccgacccg tttccccaca ctgctcgtca acggctcgtc
    16981 cggcatcgcc gtcggcatgg cgaccaatat cccgccgcac aacctttccg ataccgtcaa
    17041 tgcctgcctg cgcctgctcg atgcacccga caccgaaatc gacgaactga tcgacattat
    17101 ccaagccccc gacttcccga ccggggcaac catctacggc ttgagcggcg tgcgcgaagg
    17161 ctataaaaca ggccgcggcc gcgtcgttat gcgcggtaag acccatatcg aacccatagg
    17221 cagaaacggc gaacgcgaag ccatcgttat cgacgaaatc ccctatcagg tcaacaaagc
    17281 caagctggtc gagaaaatcg gcgatttggt tcgggaaaaa acactggaag gcatttccga
    17341 gctccgcgac gaatccgaca aatccggtat gcgcgtcgtt atcgagctga aacgcaacga
    17401 aaatgccgaa gtcgtcttaa accaactcta caaactgact ccgctgcaag acagtttcgg
    17461 catcaatatg gtggttttgg tcgacggaca accgcgcctg ttgaacctga aacagattct
    17521 ctccgaattc ctgcgccacc gccgcgaagt cgttacccga cgtacgcttt tccggctgaa
    17581 gaaggcacgc catgaagggc atatcgccga aggcaaagcg gtcgcactgt ccaatatcga
    17641 tgaaatcatc cggcttatca aagaatcgcc caacgcggtc gaggccaaag acaaactgct
    17701 tgcgcaccct tggcgcagta gcctcgttga agaaatgctg acgcgttccg gtctggattt
    17761 ggaaatgatg cgtccggaag gattggctgc aaacatcggc ttgaaagagc aaggttatta
    17821 cctgagcgag attcaggcag atgctatttt acgcatgagc ctgcgaaacc tgaccggcct
    17881 cgatcaagaa gaaattgtcg aaagctacaa aaacctgatg ggtaaaatca tcgactttgt
    17941 ggatatcctc tccaaacccg aacgcattac ccaaatcatc cgcgacgaac tggaagaaat
    18001 caaaaccaac tatggcgacg aacgccgcag cgaaatcaac ccgttcggtg gcgacattgc
    18061 cgatgaagac ctgattccgc aacgcgaaat ggtcgttacc ctgacacatg gcggctatat
    18121 caaaacccag ccgaccaccg actatcaggc gcagcgtcgc ggcgggcgcg gcaaacaggc
    18181 ggctgccacc aaagacgaag actttatcga aaccctgttt gttgccaaca cgcatgatta
    18241 tttgatgtgc tttaccaatt tgggcaagtg tcattggatt aaggtttaca aactgcccga
    18301 aggcggacgc aacagccgcg gccgtccgat taacaacgtc atccagttgg aagaaggcga
    18361 aaaagtcagc gcgattctgg cagtacgcga gttccccgaa gaccaatacg tcttcttcgc
    18421 caccgcgcag ggaatggtga aaaaagtcca actttccgcc tttaaaaacg tccgcgccca
    18481 aggcattaaa gccatcgcgc tcaaagaagg cgactacctc gtcggcgctg cgcaaacagg
    18541 cggtgcggac gacatcatgc tgttctccaa cttaggtaaa gccatccgct tcaacgaata
    18601 ctgggaaaaa tccggcaacg acgaagcgga agatgccgac atcgaaaccg aaatttcaga
    18661 cggcatcgaa gatgaaaccg ccgacagcga aaacgcactg ccgagcggca aacacggtgt
    18721 tcgcccgtcc ggtcgcggca gcggcggttt gcgcggtatg cgcctgcctg ccgacggcaa
    18781 aatcgtcagc ctgattacct tcgcccctga aaccgaagaa agcggtttgc aagttttaac
    18841 cgccaccgcc aacggatacg gaaaacgcac cccgattgcc gattacagcc gcaaaaacaa
    18901 aggcgggcaa ggcaatattg ccattaacac cggcgagcga aacggcgatt tggtcgccgc
    18961 aaccttggtc ggcgaaaccg acgatttgat gctgattacc agcggcggcg tacttatccg
    19021 caccaaagtc gaacaaatcc gcgaaaccgg ccgcgccgca gcaggcgtga aactgattaa
    19081 cttggacgaa ggcgaaacct tggtatcgct ggaacgtgtt gccgaagacg aatccgaact
    19141 ctccgacgct tctgtaattt ccaatgtaac cgaaccggaa gtcgagaact gaaaatcatc
    19201 tcccgatgcc gtctgaagat tcagacggca tttattttat ccctcatccg tcatccggct
    19261 tctcacaata tagcggatta tagtcaatta aaaacaaggg gctgtcctag ataactaggg
    19321 aaattcaaat taagttagag ttgcccctat gagaaaaagt cgtctaagcc ggtataaaca
    19381 aataaactca ttgaactgtt tgtcgcaggt gtaactgcaa gaacgacagc agagttagta
    19441 ggcgttaata aaaataccgc agcctattat tttcatcgtt tacgattact tatttatcaa
    19501 aacagtccgc atttggaaat gtttgacggc aaagtagaag cagatgaaag ttattttgct
    19561 gaacgacaaa accatattaa tggaattgag aacttttg
//
LOCUS       DAXHKX010000020        19596 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHKX010000020 DAXHKX010000000
VERSION     DAXHKX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.020"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(396..3002)
                     /gene="acnD"
                     /locus_tag="ACFMWZ_000609"
     CDS             complement(396..3002)
                     /gene="acnD"
                     /locus_tag="ACFMWZ_000609"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC5934606.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKTQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGREIRLKDIWPT
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSLARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIAAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(3080..3868)
                     /locus_tag="ACFMWZ_000610"
     CDS             complement(3080..3868)
                     /locus_tag="ACFMWZ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC5934607.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLTDERNPLYQMIISMF"
     gene            complement(4029..5183)
                     /gene="prpC"
                     /locus_tag="ACFMWZ_000611"
     CDS             complement(4029..5183)
                     /gene="prpC"
                     /locus_tag="ACFMWZ_000611"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC5934608.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5269..6144)
                     /gene="prpB"
                     /locus_tag="ACFMWZ_000612"
     CDS             complement(5269..6144)
                     /gene="prpB"
                     /locus_tag="ACFMWZ_000612"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC5934609.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6770..8080)
                     /locus_tag="ACFMWZ_000613"
     CDS             complement(6770..8080)
                     /locus_tag="ACFMWZ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC5934610.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREILVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWVVAVLWALKFWYLG"
     gene            complement(8219..9397)
                     /gene="ftsZ"
                     /locus_tag="ACFMWZ_000614"
     CDS             complement(8219..9397)
                     /gene="ftsZ"
                     /locus_tag="ACFMWZ_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC5934611.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9516..10760)
                     /gene="ftsA"
                     /locus_tag="ACFMWZ_000615"
     CDS             complement(9516..10760)
                     /gene="ftsA"
                     /locus_tag="ACFMWZ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC5934612.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10846..11574)
                     /locus_tag="ACFMWZ_000616"
     CDS             complement(10846..11574)
                     /locus_tag="ACFMWZ_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC5934613.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11564..12478)
                     /locus_tag="ACFMWZ_000617"
     CDS             complement(11564..12478)
                     /locus_tag="ACFMWZ_000617"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC5934614.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12591..14000)
                     /gene="murC"
                     /locus_tag="ACFMWZ_000618"
     CDS             complement(12591..14000)
                     /gene="murC"
                     /locus_tag="ACFMWZ_000618"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC5934615.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(14155..15222)
                     /gene="murG"
                     /locus_tag="ACFMWZ_000619"
     CDS             complement(14155..15222)
                     /gene="murG"
                     /locus_tag="ACFMWZ_000619"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC5934616.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVQAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15226..16551)
                     /locus_tag="ACFMWZ_000620"
     CDS             complement(15226..16551)
                     /locus_tag="ACFMWZ_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC5934617.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16677..18014)
                     /gene="murD"
                     /locus_tag="ACFMWZ_000621"
     CDS             complement(16677..18014)
                     /gene="murD"
                     /locus_tag="ACFMWZ_000621"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC5934618.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAITGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCDLN
                     MTDCATLEEAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(18104..18376)
                     /locus_tag="ACFMWZ_000622"
     CDS             complement(18104..18376)
                     /locus_tag="ACFMWZ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC5934619.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            complement(18491..19573)
                     /gene="mraY"
                     /locus_tag="ACFMWZ_000623"
     CDS             complement(18491..19573)
                     /gene="mraY"
                     /locus_tag="ACFMWZ_000623"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC5934620.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4666 a         5441 c         4958 g         4531 t
ORIGIN      
        1 cgccttgtcc cgatttttgt taatcctcta taccatgtca attcagcctt tatttcgcgg
       61 gcaaagccca cgctacaccc actttccaga agtacattag gctgccactc cgacaaagcc
      121 gtttgattcc gcaaactgtc ggggttcgcc acaatctacg gctactgtgt tgtgatacgg
      181 cagattatta aaccccgtca ttcccgcgca ggcgggaatc caaatttgtc cgcacagaaa
      241 ctcatcggat aaataaggtt tcctcaattc cactttctgg attcccgcct gcgcgggaat
      301 gacgatttac agtagatgcc tgccatatcg agaattacgt gaatgagcaa aatgttgaac
      361 ccgacccacg ctacgtcttg cttttcagac gacctctaag ccgcgttccc ttccaaaaaa
      421 tcctgtgcaa accgttgcaa cacgccgccg gcttcatata ccaatacttc ttctgcagta
      481 tcgaggcggc aggtaacggg aacttcgacg gtttcgccgt ttttacggtg aatcacgagg
      541 gtcaggtcgc agcgcggtgt acgttcgccg ataacgtcgt aggtttccgt gccgtccagt
      601 tgcagggtgt ggcggttggt gccgggtttg aattgcagcg gcaagacgcc catgccgatg
      661 agattggtgc ggtggatgcg ctcgaaacct tcggcggcga tggcttccac tcccgcgagc
      721 cgcacgccct tcgccgccca gtcgcggctt gagccttgac catagtccgc accggcaatg
      781 atgatgagcg gctgtttgcg gttcatatag gtttcgatgg cttcccacat gcgcatggtt
      841 tcgccttcgg gttcgacgcg ggcgagcgag ccttggcgca cgctgccgtc ttcgtttttc
      901 accatttcgt taaacagttt cggattggcg aaggtagcgc gttgggcggt caagtggtcg
      961 ccgcggtggg ttgcgtaaga gttgaagtct tcttcaggca aacccatttt cgccaaatac
     1021 tcgcctgcgg cactgacggc caaaatcgca ttggacggcg agaggtggtc ggtggtgatg
     1081 ttgtcgggca aaatcgccag cggacgcata cctcttaatg tgcgttcccc tgccagcgcg
     1141 ccttcccagt aaggcgggcg gcggatgtag gtggacatcg gacgccagtc gtatagcggg
     1201 cttggggctt tttgcgctgt gccggtgtcg aacatcggta catacacatc gcggaactgc
     1261 tgcggtttca catattcagc aacgacggca tcgatttctt cgtcggtcgg ccagatgtct
     1321 ttcaggcgga tttcgcggcc gtctgcaacg ccgagtacat cgttttcaat atcgaaacgg
     1381 atgctgcctg ccagcgcgta agcaacgacc aacgggggcg aagcgaggaa agcctgtttc
     1441 gcatacggat gaatacggcc gtcgaagttg cggttgcctg acagtacggc ggtggcgtac
     1501 aaatcgcggt cgatgatttc tttctgggtt ttcggatcca gcgcgccact catgccgtta
     1561 caggtggtgc aggcgaaggc gacgataccg aagccgagtt tttccatttc gggcagcagg
     1621 cctgcttctt tcaaatagat ttcggctact tttgaacccg gggcaaatga agatttcacc
     1681 caaggtttgc gtttcaagcc gaggcggttg gcattgcgtg ccaacagcgc ggcggcaacg
     1741 acgttgcgcg ggttggaagt gttggtgcaa ctggtaatcg cggcgatgat gaccgcgccg
     1801 tcgggcattt gtccgtccga aggctcttcg taaggcttcg ccagcccttt cgccgccaaa
     1861 tcggcggtcg caaaacgggc atgcgggtta cttggacctg ccatattgcg cgttacgctg
     1921 ctcaaatcaa atttcaaaac gcgaggataa acggcggttt tcaaggcatc tgcccataag
     1981 cctgcggttt tggcgtaggt ttccaccaat ttcacctgcg cgtcgtcgcg tccggtcagt
     2041 ttcaaataat caatggtttg ctcatcaata gcgaacatcg cggcagtcgc gccgaactcc
     2101 ggcgtcatgt tggaaatggt cgcgcggtcg ccgatagaca ggcttctcgc gccctcgccg
     2161 aagaattcga caaacgcccc gaccacgcgc tctttgcgca gaaactcggt cagcgccaac
     2221 acaatatccg tcgccgtaat gcccgcctgc cgtttgccgg tcagctccac accgacaata
     2281 tcgggcaggc gcatcatgga cgcgcgtccc agcattacgg tttccgcttc caatccgccc
     2341 acacccacgg aaatcacgcc cagcgcatcg acgtgcggcg tgtgcgaatc cgtgccgacg
     2401 caggtatcgg ggaacgccac gccgttttta acttgaacaa ccggcgacat tttttccaga
     2461 ttgatttggt gcatgatgcc gttgcccgcc ggaatcacgt ccacattttc aaatgcggtt
     2521 tttgtccagt tgataaagtg gaaacggtct tcgttacgtc tgtcttcgat ttcgcggttt
     2581 ttgcggaagg catcaggatc gtaaccgccg cattcaacgg ccaaagagtg gtcgacaatg
     2641 agctgggttt gcaccaccgg attcactttg gcaggatcgc cgcctttttc ggcaatcgca
     2701 tcgcgcagac ctgccaaatc caccaacgcg gtctgcccca gaatatcgtg gcacaccacc
     2761 cgcgccggat accaaggaaa gtcgatttcc tgttttccct caatcagctg acccagccag
     2821 ctttgcagcg tcggcaaatc gactttgtcc gcgcggttga ccaaattctc cgccaaaatg
     2881 cggctcgtgt aaggcagctt gtcgtaagag ccgggcttga tgtcctcaca cgccgcacgc
     2941 gcgtcgtagt attccaaatc cgtaccgggc agcggtttgc ggtaacgttg gttggcagcc
     3001 atgtcggttc tcctggggat ctgtttttct tgtggtttga ggtttcagcc gacgttttga
     3061 aggggtcgtc tgaaagggtt taaaacatcg aaataatcat ctgatacagc ggatttctct
     3121 cgtctgtcaa caatttcaca gccatcgaaa tgctgatgac aatcagcagc ggcttaatca
     3181 gcttcgaacc gaagcggacg gcaaatctcg cacctaaatt cgcaccgaca aacgcaccga
     3241 ccgccatcgt tgccgcaatc gggaaaataa tcgaaccgtg cagcaggaat accgatagcg
     3301 aaccaagatt gcaggcaacg ttcgccaatt tggtgtaaga catcgcgttc aacagcttgc
     3361 agccgagcaa aacaataaag gcaatcagaa aaaacgagcc gacacccggt ccgaacacac
     3421 cgtcgtaaaa acccaaaagt ggtgcaaccg tcagaccgaa cagaaaaaaa gacattctgg
     3481 ctttgccttc cttactgccg tcgagcttgg gcgaaaacac aaaatacagc gcgacaaata
     3541 tcaacaaaac cggcacgacc gccagcagaa tatctttgga aaccaagctg accgataatg
     3601 caccgaccac gccgcctgca aacgatgctg ccgcaatcgg gagacctttc ttccaatcaa
     3661 tcaaaccttt gcgtgcaaaa gaaaccgtag ccgaaaacgt agcagcggct gcttgcagct
     3721 tgttggtggc aattgccgac acgggaggaa tacctgccaa caagagtgca ggcagcgtaa
     3781 tcaaaccacc cccacccgca atcgcatcga taaatccggc aatcatcgca accaaaccca
     3841 aagcgagtat tatgtataaa tcttccatgt ttcttatcct gttacttgca ccaatacagg
     3901 attatcttct tctattaggt taaattttta aacttatttc agacaacctt tccaatcagg
     3961 caaggtcgtc tgaaatcctc agctttgcat accgaaatta aagaacaaac tgctattgca
     4021 ttcttcaatt atcgttcttc aatctccaca aacgccaaat cttcagggcc tgtgtagttt
     4081 gcgctcggac ggatgatttt gccgtctttg cgttgctcaa gaacgtgtgc gctccaaccg
     4141 gttgtacggg aaattacgaa cagcggtgtg aacatagcgg tcggtacgcc caatttttgg
     4201 taggaaacgg cagagaacca gtccagattc gggaacattt ttttctcttc ccacatcacg
     4261 ctttccaaac gttcggcaat gtcaaagagg cgcatatcgc cggtttcttt gctcaaaccg
     4321 cgtgccactt ctttaatgac aacgttgcga gggtcggaaa tggtgtacac cggatgaccg
     4381 aaaccgatca cgatttcttt gcggccgatg cgttcgcgga tgtcggcttc agcttcgtcg
     4441 gcattgcggt agcgtttttg aatatcgtaa gccacttcgt tcgcgccgcc gtgtttcgga
     4501 cctttcaacg cgccgattgc tccggtaatg ctggagtaca tatcagagcc tgtaccggcg
     4561 atcacgcggg cggtaaaggt agaagcgttg aactcgtgtt cggcatacag aatcagtgaa
     4621 acgtgcatgg ctttgatgtg tgattcgctt gggcgtttgc cgtgcaacag ttgcaggaaa
     4681 tgaccgccga tggtctcttc gtcgctttca acctcaatgc gtttgccgtt gtgcgaatat
     4741 tgataccagt acaagaggat gctgccgagg ctggcgatca gtttgtcggc gatgtcgcgc
     4801 gcttcacttt ccggatggct ttcacgttca ggatgaacgc agcccagcat ggatacgccg
     4861 gtacgcatta cgtccatcgg atgggtatgt gcaggcaggc tttccaaaac tttaatcaca
     4921 cggataggca ggccgcgcat ggatttgagc ttggttttat aagcggccag ctcgaatttg
     4981 ttgggcagat ggccgtgaat cagcaggtgg gcgacttctt caaactcgca tttttgtgcc
     5041 aaatccagaa tgtcgtaacc gcgatagctc aaatcgttgc cggtacggcc aacggtacac
     5101 aaagcggtat taccggccgc aacgccagaa agcgcaacgg attttttagg tttgagggtc
     5161 ggggtttgag tagtttcagt catggtattt ctcctttgtg tttttatggg tttcgggttt
     5221 tcagacgacc gatgcggatt tgttgaaagg cagtctgaaa gcggtaaatc atttttgaaa
     5281 caatttatcc agtttttgct cgaaggcatg atagttcaga tgctcgtaca gctcggcacg
     5341 ggtttgcata ctgtccacca ccgccgcctg agtgccatcg cgcataatcg cttcgtaaac
     5401 attcagagcg gctttgcttg ctgcacggaa cgatgacagc ggatacagca ccagcgacac
     5461 gccgttttca gccagctcgc tttgggtata aagcggagtg gaaccaaact cggtaatgtt
     5521 cgccaacacg ggcactttca ccgcatctgc aaattggcgg tacatgttca aatcggtcat
     5581 ggcttcaggg aaaatcatgt ccgcaccggc ttcgacacaa gcttgggcgc gttcgatagc
     5641 ggcatccaaa ccttctaccg ccagcgcatc ggtacgcgcc ataatcacga agttctcatc
     5701 aacgcgcgca tctacggcag ctttgatacg gtcgaccatt tcatctttag atacaatggc
     5761 tttgttcgga cggtggccgc agcgtttttg cgctacctga tcttcgatgt gaaccgctgc
     5821 aacaccggcg cgttcaaagt tgcgaatggt acgggcaata ttgaatgcac cgccccaacc
     5881 cacatcgatg tccaccagca gaggcgtatc cacgttgtcc gtaatgcgtc gtgcgtcgat
     5941 cagcacatct tccattgtgg taatgcccaa atcagggata ccgcaagaac aggctgccac
     6001 gccgccgccg gacagataga tggctttgaa accgctttgg gtggccaatc gtgcaaaata
     6061 agcattgacg caaccggcga cggcaagcgg attcgattct ttcacggctt ggcggaaacg
     6121 tgctccggca gagtgttgac tcatcatatt tctcctttat agactttttt tcagtatgga
     6181 caggcttcca tcacattcgg acggcaaaac acagccatcc gacgcgtcgg gcatcccaat
     6241 ccaatcatta aaaatatatg ggaaaaatta tcttattgat atttaaaacg aatcaaagaa
     6301 aaacagcaga ccgttcggaa ttatgcggca aaaccgcaga caagaagaaa acaaggggat
     6361 tattcagaaa aggggaaaac atctgaattg gtttcatagt aatgttcctt tgtagtgtta
     6421 tgtagtttta tttttcgata atctaaaaca caagactgaa aatgtcaaac atttttaagg
     6481 agactcatta cataatttta atattttatt ttaattccat gattaaacta gattaatttt
     6541 gaattatttg tttcaataaa attgtcagga aatcaagaaa aattttcgat aatcaaaatc
     6601 ctgaaaactt ataaaatatt ttatacataa aaacaatgta ttatttttaa gcaatccgat
     6661 gtattttatt atatatttga tttttatata atttaataaa aattaaatat atcggcaaac
     6721 aaaatgccgt ctgaaaacct ttcagacggc atttttaata tcgaatcaat cagcccaaat
     6781 accagaattt cagtgcccac aatacggcaa caacccatac cataggcgga acgtctttgg
     6841 tgcggcggca taaaagttta accacggcat agctgatgaa gccgaaggcg atgccgtctg
     6901 caatcgaata ggtaaacggc atgaagacaa tggtcaggaa tgcgggtgcg gcttccgtca
     6961 tatcgtccca atcaatatcc ctcgcactgc ggagcatctg cgtgccgaca taaagcaggg
     7021 cgggcgcggt ggcaaaagcg ggaacacttt tcgccaaagg tgaaaacatc aggcaggcga
     7081 gcatcaatac gccgacggta accgccgtca ggccggtccg tccgcctgcc gatacgcccg
     7141 ccgcgctttc cacataaggc gtggtggaag aagtacccaa agccgcacct gccacaatgg
     7201 cggtagagtc tgcaagcagt gcgcgtttca ggcggggcag cttaccgtcc accagcagcc
     7261 cggcacggtg ggatatgccg accagcgttc cggtactgtc aaatagatcg accaagaaga
     7321 agacgaaaat cacactgacc atgctgacgg taaacaggcc ttcaaaatcc atctgcataa
     7381 aagtcggcgc aatgctcggt acttcgccga tgatgccgtg aaattcattc aaacccatca
     7441 ggctggcaat gacggtaatg gtcaagatgg tgatgatgat tgcgccttga acgcggaaat
     7501 gtcccaatac gaccaccata gcaaaaccga acaatgccaa caacgcggac ggctgatgaa
     7561 tatcgcccaa accgaccaag gttgccggat tggcaacgat aatgcctgcg cctttcaggg
     7621 aaatcagtgc caaaaacaaa ccgataccgg cagcaatcga cattttcaaa cccataggca
     7681 gtgcgttgac cagtatttcc ctgactttaa aaaagctgaa caggataaaa atcagaccgg
     7741 agatgaacac cgcacccaac gcaacctgcc aaggcacgcc cataccctta acgacggcaa
     7801 aggtgaaata ggcattcagc cccatccccg gtgcgagtgc aatcggatag ttgccgacaa
     7861 aacccataac aaaacagccg atggcagacg cgatacaggt agcgacgaat accgccccca
     7921 tatccatgcc ggtctcgccc aaaatcagag ggttgacgat aacgatgtag cacatcgtca
     7981 aaaaagttgt caaacccgcc atcaactcgg tacgcaccgt cgtaccgttt gccttcagct
     8041 taaaaatccc gtccaacagt gtttgttttg aagtgtccat atctgaaagt ctgtatcaag
     8101 tggaaaaatc tgaatccgcc gaccgcaaac cgtacgaaat tgcaacatct tgaaaaacat
     8161 acctcccaaa ccggacaaac cgattcggga ggcagcaggt ttacgggcaa acagcacatt
     8221 atttgtctga attgtgttga cgacgcaaaa tcgcagggat ttcaaagtcg tcaagtacgg
     8281 actgattgtc gaaatccgca gcggtaaggt tcatcgtgcg gataccgcga ttggtgcgga
     8341 tcataccttc gacattgtgg ctttgctcct gtttggacgg agcaaccgct tctacctccc
     8401 ttgccggaac aaaatcgacc gcgccttttt ctttcagacc ggtagcgata atggtaatcc
     8461 ggatggcatc ttcgctcatg gtctcgtctt cagccgcacc gaatttgcat tccaaatcgg
     8521 gatgcgcgct ttggttgacg attttcatga cttcggacaa ctcggacatt ttcaagcaac
     8581 ccggagcagt cgtaatattg accagcacac cgcgcgctcc gtccaaggtt acatcgtcca
     8641 gcagcggact ggaaatggcc tggtcggtcg ccatacgcgc acggtcgata ccttgggcat
     8701 aacccgagcc catcatagcg ataccgcggt tgctcatcac ggttttcacg tcggcaaagt
     8761 cgaggttgat gatttcgctc gggcaagtta ccacttcgga aatgcctgcg accgcatcgc
     8821 gcaatacatt gtcggcggca cggaaggctt cgcgcatcgt tacgtcttca cccaatgcag
     8881 tcatcagttt gtcgttcggg atgatgatca gcgaatcgac gtgttctttc aactgttcca
     8941 accctgcctg tgcgacatgg acgcgcttac cttcatatgc gaacggtcgg gtaaccacgg
     9001 caacggtcag aatgcccaaa gacttggcaa tctcagcaac aaccggcgcg gaaccggtac
     9061 cggtaccgcc gcccataccg gtcgtgataa acagcatatt cgcaccgcga atggcttctt
     9121 caatggcttc ccggtcttcc tgggctgccg cacggccgat atcgggattc gcgcccgcgc
     9181 ccaaaccgcg tgtcagattc gtacccaact ggattctctt cgccgcatgg ttttttgcca
     9241 gagactgcgc atccgtattg gcactgataa actccacacc gcgcacattg ttggcaacca
     9301 tgttattgat tgcattgcaa ccgccgccgc ccaagccgat tactttaatc accgcagggc
     9361 tgactgccga ttctgccacg tcgtaaacaa attccattca aaaactcctg ctcgccccat
     9421 tcagaggacg gtttaaataa attattattc attatataag aagtatcttg ctgctggcaa
     9481 aatacttctc acctgtcaaa cggcaatcca cctgttcaga agctgttttc aatccaccgt
     9541 ttcaatcttg ccaacaaacc gccgccccct tccctctctt gcactgcacc gttttccggc
     9601 tgcggcaagt ttccttccag cttgcatgct gcatgaagca gcccgatagc ggtagaaaaa
     9661 cgcggtgtgc ggacgcggtc ggacaaaccg cccatttctt ggggtgcacc ggtgcgtaca
     9721 ggcaaatcga agattttttc ggcaaattcc acaatcccgg tcatcatgga cacaccgccg
     9781 gtcagaacga tacccgcatt cagcacttct ttggggaaac ccgatttttg cagctcgccc
     9841 agcactacgc caaaaatctc ctgaatccgt gcactgatga ttgctgccag aaccttactg
     9901 gaaacctgac gcgatgtccg gtcacccacg cccggaactt caatcatctc acccaagcct
     9961 tccgtatcgc atgatgccac gccataatgg attttaatgt actcggcggc atcgagaggt
    10021 gttctcaacg atttggacaa atctttggta atcagattac caccggccgg aatgacggac
    10081 gtatggcgga tggcaccgtt catataaacg gcaatatcgg tcgttccgcc accaatgtcg
    10141 atgacgcata cgccgaggtc tttttcatct tcagtcagca ccgcctgccc gcttgccaac
    10201 ggctgaagca tgatttgatc gcttttcaag ccgcaccgct cgatacattt ttggacattc
    10261 tgcactgccg tacttgcacc ggtaatgatg tgcacccgcg tatccagacg cacaccgctc
    10321 ataccgatgg gctccctcac gccaagttgg gtgtcgataa tgtagtcttg aaccacggca
    10381 tcgagaattt tttgatcggg cgggatattg attgcctttg ccgtttcaat ggcgcggtcg
    10441 atgtctgcct gcgtgacttc cccgtcttta attttaacca caccttgcga attgagactg
    10501 cggatgtggt tgcctgcgat acctgtggta acgtgggtaa ttttggtatc cgccatcagc
    10561 tcggcatcat tgaccgcctg cctgatggct tggacggtgg catcgatatt ggttaccatg
    10621 cccgcgcgca agccccgtga aggagcctgc cccaaaccga cgatgttgat tttgtcgtca
    10681 tcttgaactt ccccgatcag tgcgaggact ttagacgtac cgatatccag tacgctgatg
    10741 tatctttgct gctgttccat tgttcgtctg ctcttaaaac tgattgaaat ttgcgtcgca
    10801 ccatttcaga cggcacggcc gtaatctgtc cgatacctgt tcccactatt cttcggattc
    10861 tttttcgggt aaaccgtcgg aagcatagcg gactgaaaat ccgtccttat acctcatatc
    10921 cacataggat aaccgatttt tatttttacg caacagatgc tgccacgctt cggtaaaaag
    10981 ccggaggcgt ttcatctcgt tttcccgtcc gagcctgacg gtgatgccgt tgtccaaaac
    11041 gacaatccac gccgaacgtg ccgtataggt catctctttg atgcccaaac cctgttttgc
    11101 caaaacagtc gaaaattcgt cataacggcg gagcatttcg gcagacgttc cttccgcgcc
    11161 tctgaatacc ggcattccgg gtctgtccaa gcgggcttca aaaacattgc cttcgccgtc
    11221 caccaaggca tggtcgcccc aacgcgcgac cggcttgcgc tcggtcagga cgacctcaac
    11281 cgtgtcggga aaacggcggc gcaccatgac cgacgcaatc cacggatacc ggcggtaggc
    11341 ttcctgtgcg ccattgatgt ccgtcctcaa aatattccca tggatgtatt ctttcgccaa
    11401 actgcccaat gccttcttat cggaataaac caggttgccc ttcagcgaca cctgcttgac
    11461 gggcagatga ttcgaattgt aaaaccaaac cagcccggac gcagcaagca gcatcgccat
    11521 catgacaagc agccagcgcg tcagccgttc catcgcttcg gcattatccc acatgtgcgg
    11581 tcttcaaaat ttcaatacat aaatcggcaa aacccacgcc cgtaacggca gcggatttcg
    11641 gtactaaact atggctcgtc ataccgggca gggtgttgat ttccaacaga tagagtttgc
    11701 cgtcggtatc tttgaggaaa tcgacgcgca cgcagccttc cgcaccgatt gcctgcgcgc
    11761 cgcgaaccgc cagttcgcgc atcaggcttt cttcggcttc ggtcaaatct tccgaaggac
    11821 attgataaat ggtgtcgtcg cggttgtact tggcttcgta gtcgtaaaac tcggttgcgg
    11881 gaatgatgtg tatgccgggc agccctttgc cgttcaggac ggggcaggaa tattcgccgc
    11941 cgccgataaa ccgttcggca atgatttcgc cctgaaggtg tttcaattct tcgtaaacgc
    12001 ttttcagacg gccttttcct ttgactttta ccacgcctac gctgctgcct tcggccgccg
    12061 gtttcacaaa catcggcagg cccaattttt cttcgacggc atcgaaatca gtgtcgtcgt
    12121 gcaggacggc gaactcggga acgggcagtc ccaatgcctg ccaaatcagt ttgcagcggt
    12181 atttgtccat gccgatggcg gatgcggcga caccgctgcc ggtataggga atgcccaaca
    12241 gttccaatgc accctgaacc gccccgtctt cgccgtaagt accgtgaagg atgttgaatg
    12301 ccgtctgaaa accttgtgcc ttcaattcag acaatggggt ttctttagga tcgaaggcgt
    12361 atgcgtctat gcctttgctt tttaaagcat tcaaaatggc ggtgccgctg tccagcgaga
    12421 tttctcgttc gctggaaaaa ccgcccatca atacggccac tttgccaaaa ttctgcattg
    12481 tttttgttct ttcctgattg ctttatgctt gttgccagag gtcgtctgaa acctgatttg
    12541 cggtttcaga cgacctttat atgatgttcc gtctgtcagg cgggtgtgcc tcaaatctgt
    12601 ttcgacaatt ccagcagcgc ggcggggacg cggttgatgc ttcccgcacc catattcaac
    12661 acgatgtcgc cgtcctgcaa aacgttcaac agcatttcgg gcagatcggc aacgttttcg
    12721 cagtaaatcg gctcgagttt gcccaacacg cggatggcgc gggcaagagc gcgggaatcg
    12781 gcggcggcaa tcggctcttc accggcggca taaacttcgg tcagcaccag cgcgtcaacg
    12841 gtattgagga ctttggtaaa gtcttcaaac aaatcgcgcg tgcgggtata gcggtgcggc
    12901 tggaaggcga gtaccaaacg tttttccgga tacgcgccgc gtgcggcggc aagggtcgcc
    12961 gccatttcga cggggtggtg tccgtagtcg tccaccaaga gcgcggtccc gccgtttggc
    13021 aacttgatgt cgccgtattt ttggaagcgg cggccgacac cttcaaagcc gagcaagcct
    13081 ttttggatcg cttcaaccga tgcgccgact tccagcgcca cgccgatggc tgccaatgcg
    13141 ttcagcacgt tgtgtctgcc gggcatattc agcacgactt caaacgaccc ctgctcatgt
    13201 cctttcattt gaacatggac ggtgaatttc atttgcgcgc cgacgttttc gatgtcggtg
    13261 gcgtagatgt cggcggtatc gtccaaaccg taagtagcat aaggtttgct cactttgggc
    13321 aaaatcgcgc ggacgtgttc gctgtcaata cacaaaaagg ctttgccgta gaagggcata
    13381 cggtggatga aatcgataaa cgcctgatgc agtttttcga cgctgtgccc gtaggtatcc
    13441 atatggtctt cgtcgatatt ggtaacgacg gacataatcg gtgtcaggtg cagaaaggat
    13501 gcatccgact cgtcggcttc ggcaacgatg tattcgcctt tgcccaagcg ggcgttggtg
    13561 cctgcggcgt tgagtttgcc gccgataacg aaagtcgggt caagtcctgc cgcgccgagg
    13621 atggaggcgg tcaggctggt ggtcgtggtt ttgccgtgcg tgccggcaat ggcgatgccg
    13681 tcacggaagc gcatcaactc cgccaacatc agggcgcgcg gaataacggg aatttgctgc
    13741 tccaacgcag cgacaacttc gggattttct tttttgacgg cggtagaggt aacgacgaca
    13801 tccgcaccgt taacgtgttc ggcggtatgg ccgggataaa cttgaatgcc caggctgccc
    13861 aaatgctcgg tagcggcatt acgcgcctga tccgaaccgg aaactttaaa acccaaattg
    13921 tgcaagactt cggcgatgcc gctcatgccg acgccgccga taccgacaaa atggatgttg
    13981 gtaactcgat ttttcatcat aatgttgcgt tccggtggat tttcgatgcg taaaggcgtt
    14041 attttaaagg gctgaccgtt tgcgcgccat agttttctga caaatatata gcggattgaa
    14101 ataaaaaaca tccatgctgt ctggacggca tttcagacgg catggttcgg cagtttacgc
    14161 cgcacacgca atcgcggctt cggcaacgtc gtccgcgctg tgcggcaatg ccaaagtacg
    14221 ggcgttttcc gcccatttga gacatttttc gcggttcagg ctgccgagga tttcggcgag
    14281 tttttccgcc gtcaactggg tttgcggcaa cagcaatccc gcctccgcct gcaccataaa
    14341 acgcgcgttg gcggtttgat ggtcatcaac ggcgtgagga tacggcacta acaacgcacc
    14401 caatcccgcc gccgtcaact cggcaatcgt cagcgcgccg gcacggcaaa tcaccaaatc
    14461 ggcatcgcgg taggcggaca ccatatcggt aataaattcc acgcattccg cttgcacgcc
    14521 cagcgcgtcg taatccgcct gcaagctgcc cagcttgccc cgtcccgatt ggtggtacat
    14581 ctgcggacgc gcattgtcgg gcagcaaagc caatgcctgc ggtacggttt tgttcaaaac
    14641 gtccgcgccc aaactgccgc cgaccaccaa aattttcaga cggccttcac gcccttggaa
    14701 gcgttcggca ggcacgggca ggttgctaat atcggcgcgg acggggttgc cgaccaagcc
    14761 gccttcgtgg ctgaacgctt tcggaaaagc gtacaacacc cgcttcgccc agcgcgacag
    14821 gtggcggttg gacaaacctg ccacggcgtt ttgctcgtga atcacaatcg gcacgcctaa
    14881 tagcttcgcc gccaaaccgc cggggaaggt aacgaagccg ccgaagccga tgacgcactc
    14941 gacacggtgt ttgcggataa tccgctgcgc ttcgcggacg gtttgataca aagtaaacgg
    15001 cagcatcagc ttgcgtttga tgccgttgcc gcgcacgcct ttaatcgcca gcgtttccaa
    15061 gcgtatgccg tattgcggca cgatacgctc ttccatcgaa tccttgctgc ccagccaaat
    15121 cacatgatgg ccgcgcgcgc gcaatgaatc cgccaccgcc agcgcgggga aaatatgtcc
    15181 gcccgttccg cccgccatca gcataaaggt tttaccgccc atgatttact ccacccgata
    15241 accgcgcatt ttccggcggt tttcataatc tatacgcaac agcagcatca tgctgatcag
    15301 catgaaaaag actgacgaac cgccatagga catcaacggc agcgtcagac ctttggtcgg
    15361 caaagcaccg atgttcacac cgatattgaa gaaactttgg ataccgatcc aaatgccgat
    15421 acccgaagcg atataggcgt tgaaagtcaa acccaaatcg cgcgactgct tgccgatgga
    15481 aaacgcccgc accaccagcc agccgtaaca gaatatcagc acacacatac cgaagaaacc
    15541 gaattcttcg gcgatgatgg caaaaataaa atcggtatgc gcttccggca gaaagccgcg
    15601 tttgctcaaa ctcgcaccca aacccatacc gaaccactct ccgcgcccga ttgccatcag
    15661 agagtgggta agctggtagc cggcaccctg cgggtctttc cacgggtcca aaaatgccac
    15721 tacccgctgc acacggtagg gagcggcggt aatcatcagc accatcccgc ccaagacgct
    15781 gcctaccagg acgaaaaaat atttccacgg caatcctgcc aaaaacagca ttccaacggc
    15841 aatgacggta atgacgacaa acgaaccgaa atccggctgt accattatca gcaccaaacc
    15901 gaacgccacc agcataatcg gcaggatgat cgcccggaaa cggccgtaca tttctaatgt
    15961 ttcacgacgt gcctgcggat tggtggcgga catgatcaga ttggccgtcc cccgccaaat
    16021 cgactgccaa cccaaacttt ccatgctgcg caacacttct tcacggcgcg tgaacaggct
    16081 tgccaaataa aggatgaccg ccagcttgaa cagctcggtc ggctggaaat tcaacggacc
    16141 caaaggtatc caacgggtcg cgccattgat ttcgcgcccg gcaatcaata cgactaccag
    16201 caacaggccg gatagggcaa aaatccacgg cacaagccgc cgccatgtcc tcatcctgca
    16261 aagaaaccat aaaaaaccgc tcgctatcaa gccggcaacg acgaaccccg cctgtctggt
    16321 caaatagaaa aactgatcgc cgccttcctt cgatgccaaa tacacagaag ccgaataaat
    16381 catcagcagg ctgaacgccg tcatcagcac caccatccac aaaagcggcg cgtcgaattt
    16441 cctgccgtcg cgcacaatcg gcctgtcgag cagcagagtg tggacaccgt cgcccacttt
    16501 taccaatact tccgaaatct tcaaaaaaac cacctgccag tctgtttgca cctgccgcaa
    16561 agggcaaaaa tttcagacgg cagacaatgc cgtccgaaca tacgatacat cccaaatcgg
    16621 tattctaaat ctttacttgc cgcccaacaa tgacggcgtt tgcgtttcag acggtatcac
    16681 aaagccttaa acgccccgat aaacacttcc gaacggtgcg catagccttt aaacatatcg
    16741 aagcttgcgc aggcggggct gagcaacacg atgtcgcccg cttcggcttg ggcatatgcc
    16801 ttctgaaccg cttcttccaa agtggcgcag tcggtcatat tcagatcgca gccgtccaaa
    16861 tcgcggcgga tttgcggcgc atcgacaccg atcaggaaca cgccttttgc cttgccggca
    16921 agcgcgtcgc gcagaggcgt gaagtcctgc cctttgccca tgccgcctaa aatcacgaag
    16981 agcggatttt gcaaaccggt aatcgcggcg gcggtcgcgc cgacattcgt gcctttgctg
    17041 tcatcaatga ataccacgcc gtttttcttg ccgatttttt ccacgcggtg cggcaggcct
    17101 tggaaggttt tgacgtgttc aagcaatgct tcgcgcggca aaccgacggc ttcgcacaag
    17161 gcaacggcag ccatcacgtt agtggcgttg tgcaagcctt gcaacggaat atcttgcgtg
    17221 gcaatcaaat cttcattgcc ttgtttcagg cggcctgtct cgcgttccaa ccaaaaatcg
    17281 gcttcgtgtt ccaacgaaaa ccatttcacc tcgcgcccgg cgcgcttcat cgcacgacag
    17341 aacgcatcgt ccgcattcaa aacctgcacg ccatcgccac ggaaaatctt ggctttggta
    17401 tgcgcgtagt cgagcaagtc gtcgtagcgg tcgagatggt cttcggaaat gttcagcacc
    17461 gtcgccgcag tcgggcgcag gctttcggtg ttttccagtt ggaagctgga aagctccaac
    17521 acccacacgt ccgccttttt gccttcgcgc tgccattccg cctccaaaac cggcgtgccg
    17581 atattgcccg cgataacggt atccagcccg cacttgatac agagatagcc gaccaggctc
    17641 gttaccgtgg ttttgccgtt gctgccggta atcgcaatta ccttgtcgcc ccggcggttc
    17701 acaatgtccg ccagcaattc gatgtcgccc aacacgcgtc cgccgttttg cttgaacgcc
    17761 tcaatatccg gctgacgctc gctgatgccg gggctgagtg ccaaaatatc gaaaccgttg
    17821 tccagcgcat ctttcagacg gcctgtgtaa aacaccagcc cgtcaaacat cttaccgatt
    17881 tgcgacacgc gctcgggctt caactccgca tcataagcgg ccacctccgc gccgtttttg
    17941 cgcagatagg caatcatgga aatacccgta ccgccgagtc cggcgacgag gatttttttg
    18001 ttttcaagcg tcattttggt tttgtcctaa aacaaatcat attgggcagg tggggggatg
    18061 tccgcccctg cccaagccgc tttcagacgg catcgcgggc tgttcaataa cctgccttca
    18121 ggcgttggta aatgtcgcag ccgtcttggt ctccgttttg acaagcctta ccaaaccatt
    18181 cttgagcgag cacaaggtct tggcgcacgc cgcgtccttt gtaatacatt gcgcccaaat
    18241 tgtattgggc tgctgcattt ccctgtgctg ccgcctgcaa gttttcccga aaatccgata
    18301 cgtcatccgc ccacaccgct tggttcaagc ccaaggcaat cagggcggcg gcaagccatt
    18361 tgactgtctg tttcatggtt ttacttctgt tttagtataa gccgccgtta acgatagggc
    18421 tgggcggatt gtcgccgcag gtttattgcg cgttcaaatg ccgtctgaaa ggtgttcaga
    18481 cggcataggt tcagcggatt ttgagggtac tcaaaccgat caacaccaag acgatggtaa
    18541 taatccaaaa gcggacgacg acttgggttt ctttccagcc tttttgttcg tagtggtgat
    18601 ggatgggcgc catcaggaag atgcgttttt tggttttctt ataccagcca acctgaagca
    18661 taacggatac ggcttctacg acaaataatc cgcccataat gacgaggaca aactcttggc
    18721 ggacgatgac ggcgacggta ccgagcgcgg cacccaatgc caatgcaccg acatcgccca
    18781 taaagacttg cgcgggatag gcgttaaacc acaagaaacc gaggcacgcg ccgcacatgg
    18841 cggtacagaa aatcaccact tcgtttgcgc cggcaacgta aggtaattgc aggtattggg
    18901 caaattgtga gtggccgctg gcataggcga agatggcgag gccggcggca acgaggacga
    18961 cggggaaggt cgcaaggccg tccaagccgt cggtgaggtt gacggcattg gatgtgccga
    19021 cgatggtcag gtaagacaac accaaaaagc cgaccacgcc cagcggcagg gcgatttgtt
    19081 tgaagaacgg gacaatcaaa atattgttgg cggaattggc ggcaaggtaa aacaatgcca
    19141 aactggcgat aatggcaacg cttgactgcc acaccatttt gaatttggcg gacacgccgt
    19201 tggggtcttt atagacgact ttgcgccagt cgtcgtaaaa accgagtgcg cccgtggcga
    19261 gcaatacgcc caagagaatc cagatatacg ggtttgccca gttgccccac aacagggtgg
    19321 acacggtaat ggcggtcaga atcagcgaac cgcccatcgt cggcgtgccg tttttgacga
    19381 ggtgggtttg cggaccgtcg gtacgcactg cctgcccgca tttgagcgcg gtcagcctgc
    19441 gtatcgtcca cgggccgaac atcagggaaa acgctaaggc ggtcaacgcc gccatgacgg
    19501 cgcggaatgt ggtgtattga aaaatattca gaccggttaa ccagttgctg aaatgtgcga
    19561 gccataaaaa catggggctt cctttttttg ttttgt
//
LOCUS       DAXHKX010000021        19288 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHKX010000021 DAXHKX010000000
VERSION     DAXHKX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.021"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(348..2123)
                     /gene="ptsP"
                     /locus_tag="ACFMWZ_000624"
     CDS             complement(348..2123)
                     /gene="ptsP"
                     /locus_tag="ACFMWZ_000624"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC5934621.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYNAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNIAQLENEIAKIVRCEDE
                     EKAEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(2123..2392)
                     /locus_tag="ACFMWZ_000625"
     CDS             complement(2123..2392)
                     /locus_tag="ACFMWZ_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC5934622.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2461..2898)
                     /locus_tag="ACFMWZ_000626"
     CDS             complement(2461..2898)
                     /locus_tag="ACFMWZ_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC5934623.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2979..3542)
                     /locus_tag="ACFMWZ_000627"
     CDS             complement(2979..3542)
                     /locus_tag="ACFMWZ_000627"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC5934624.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3611..4435)
                     /locus_tag="ACFMWZ_000628"
     CDS             complement(3611..4435)
                     /locus_tag="ACFMWZ_000628"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC5934625.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLVSRQGYVFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRYGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            4527..5159
                     /locus_tag="ACFMWZ_000629"
     CDS             4527..5159
                     /locus_tag="ACFMWZ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC5934626.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5366..7150)
                     /gene="rmuC"
                     /locus_tag="ACFMWZ_000630"
     CDS             complement(5366..7150)
                     /gene="rmuC"
                     /locus_tag="ACFMWZ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC5934627.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7489..8277)
                     /locus_tag="ACFMWZ_000631"
     CDS             complement(7489..8277)
                     /locus_tag="ACFMWZ_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC5934628.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8292..9641)
                     /locus_tag="ACFMWZ_000632"
     CDS             complement(8292..9641)
                     /locus_tag="ACFMWZ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC5934629.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9660..10241)
                     /gene="petA"
                     /locus_tag="ACFMWZ_000633"
     CDS             complement(9660..10241)
                     /gene="petA"
                     /locus_tag="ACFMWZ_000633"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC5934630.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10364..11113)
                     /locus_tag="ACFMWZ_000634"
     CDS             complement(10364..11113)
                     /locus_tag="ACFMWZ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC5934631.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11244..12173
                     /locus_tag="ACFMWZ_000635"
     CDS             11244..12173
                     /locus_tag="ACFMWZ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC5934632.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPIWPQIELDIVSGFQADPIGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12429..12821)
                     /gene="rpsI"
                     /locus_tag="ACFMWZ_000636"
     CDS             complement(12429..12821)
                     /gene="rpsI"
                     /locus_tag="ACFMWZ_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC5934633.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12831..13262)
                     /gene="rplM"
                     /locus_tag="ACFMWZ_000637"
     CDS             complement(12831..13262)
                     /gene="rplM"
                     /locus_tag="ACFMWZ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC5934634.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13440..13619)
                     /gene="ssrS"
                     /locus_tag="ACFMWZ_000638"
     ncRNA           complement(13440..13619)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFMWZ_000638"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13625..13930)
                     /locus_tag="ACFMWZ_000639"
     CDS             complement(13625..13930)
                     /locus_tag="ACFMWZ_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC5934635.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13941..14270)
                     /locus_tag="ACFMWZ_000640"
     CDS             complement(13941..14270)
                     /locus_tag="ACFMWZ_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934636.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14325..15314)
                     /locus_tag="ACFMWZ_000641"
     CDS             complement(14325..15314)
                     /locus_tag="ACFMWZ_000641"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC5934637.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15437..18139)
                     /gene="ppc"
                     /locus_tag="ACFMWZ_000642"
     CDS             complement(15437..18139)
                     /gene="ppc"
                     /locus_tag="ACFMWZ_000642"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC5934638.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18182..19075
                     /locus_tag="ACFMWZ_000643"
     CDS             18182..19075
                     /locus_tag="ACFMWZ_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC5934639.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDVGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGTER"
BASE COUNT         4138 a         5251 c         5124 g         4775 t
ORIGIN      
        1 gagcaactga aaccactctg ccgtcattcc cgcgaaagcg ggaatctaga acccaacgcg
       61 acaaaaattt atccgaagcg acaacaatct tttcatcgtc attcccgcaa aagcgggaat
      121 ctagaacgta aaatctaaag aaaccgtttt acccgacaag tttccgtgcc gacagaccta
      181 gattcccgcc tgcgcgggaa tgacggattt taggtttctg attttggttt tctgtttttg
      241 agggaatggc cgattttggg tttctgtttc ggttttctat tttgcaggaa tggcaaaatt
      301 tcagattgcg ggcattgtta agtatttcta ttttttacct gccgtattta tttccgcccc
      361 ttgaagtcgg cttcttcctc gacagacacg ctgttcatct gtttgatcag cttttccgcc
      421 ttttcctcgt cctcgcagcg gacgattttg gcaatctcgt tttcgagctg tgcgatattg
      481 ctgtgcagga tgatgttttt gacgggcagg atgttgttcg gattcatgga aaaacggcgc
      541 agccccatac caagcaggat gcgggtaaac gccgtatcgc ccgccatctc gccgcatacg
      601 gacacgtctt tgtccatgcg gttggcggtg cggatgacgt gttgcagcat tttcagcacg
      661 gcgggatgtc cgggctggta aagatggctg acgctgtcgt cgccgcgatc gacggacaag
      721 atgtattgaa tcaggtcgtt ggtgccgacg gagatgaaat cgaccagttt caaaatactg
      781 ccgacggtca gcgcggcaga cggaatttca atcatacagc cgatgccgac tttaccgaag
      841 gcatcgccgc gttcggcaag ctggcgttgc gcggtgtcga ggtggatgag gcactggcgc
      901 acttcggata cggaggtaat catcggccac atcatgcgga cgggaccgtg taccgccgca
      961 cggaggatgg cgcgcatctg ggtgcggaac atgaccggtt cggcaaggca caggcggatg
     1021 ccggtcatgc ccagcgcggg gttgaggctg ccgttgggcg tgctgttttt cccgaaccag
     1081 cgcgggtttt tgtccactcc caaatcgact gtccgtatcg ttacgctttt gcccttcatt
     1141 tttttgacaa tcgcgttgta cacttcgtac tgctcgtctt cagacggcat cgtatcgcgg
     1201 ttgaggtaaa gaaactcgct gcggaacagc ccgatgccgt ctgcgccgag gttgtgcagc
     1261 ggtttcacgt cttcggcgga ttctatattg cccacaagct cgatgcagac cccgtcggcg
     1321 gtggcggcgg cggttttttt gagcttgttc aaatcgcgtt tgtggctgcg gtattcgcgg
     1381 gcacggcggc ggtattcgtt caacaccgac tcatccggcg cgataatcaa cacgccgttg
     1441 ataccgtcca caatgaccgt ttcgccttcg gtaatcagtt tgcgcgcgtt gtgcagcccg
     1501 acgacggacg ggatgtccaa gctcctgccc aaaatcgccg tatgcccggt ggggccgccg
     1561 gcatcggtaa cgaaggcggc aatgcgctgc tctttaaaca aaaccgtgtc ggcgggcgaa
     1621 aggtcgtttg caatcagaac ggtttcgtca aacaggttgt cggcaacttc caactcgttg
     1681 ccctgcccga tcaggttgtt gtggatgcgg cggacgactt gcagcatatc ctgcttgcgt
     1741 tcgcgcaaat aggcatcgtc catattgtcg aattgggcgg cgagtttgtc gctctgctgc
     1801 ttcaatgccc actcggcgtt gattttttgt tcccttaaaa tatcgacggg ttcgcgcgac
     1861 aaggtaacat cggtcaagag catcaggtgc agcgagatga acgcgcccaa ctcggtcggg
     1921 gcgttttcgg gaatcgcgct gcggagctgt tccaactctt tgcgcgtggc tttgacggcg
     1981 gcatcgaaac gttcggcttc ggcatcggtg tccgcctccg caacatcata ctgcggcact
     2041 tcctccgtac cgcgcgcaat caggtgggcg caaccgacgg caatgccttt gcccgccgcc
     2101 acgccgtgca gcacgatact cattattcgc cctcgccgaa gtagccgttg attaagtcgg
     2161 tcagggcgcg catcgcttcc gcctcgtccg cgccgtcggt ttccagctcg atgaccgtac
     2221 ccttggcggc ggcgagcatc atcagcccca taatgctttt gccgttgacg cggctgtcgt
     2281 ttttcgtaac ccagacttcg cttttgaatt gggacgcggt ttgggtgaac ttgttggacg
     2341 cgcgggcgtg gagtccgagt ttgttgatga tttcgatgga ttgtttgagc atttcggttc
     2401 ccgtgttatg tatatcggca gcagacgccg tttaaaatgt tttcctgccc tgccgcttct
     2461 tcagacggca tcgccgctgc gccggcacac caaatcttcg ggcgcggacg tgatggcgaa
     2521 aatgcctttt acagccgcct ctctgacaca ttcggtaaag gcggcaaggt cttccgccgc
     2581 cggcgaatat tggacggcct taaccatcat cggcgcgttc agcccggtca aaatcgccga
     2641 tttgttttcg cgcacgaggc ggcgggcggc attgcagggg gtcgcgccga agatgtcggt
     2701 cataatcagc acgccgtcgt tgtcgggaaa ttcctgaagc gcggcaatgg cgttgttgat
     2761 gatgtcgtct tggtcttccg tcggttgcac gccgagtatg cggacgtttt caggcagccc
     2821 gcccgggaaa aaatgatgtg ccagcttgcg gtaggcttcg cctatggttt cgtgtgtgat
     2881 gattaaaagc cctatcatat tatgcgtcct gttcctcgtt gtcctgccgg cgtatgggcg
     2941 caatgccgtc tgaaaggcat tcagacggca tcgcgtcctt attttccgtc caatgcgtaa
     3001 atctcgccca gattgcgcca gcagcccgcc gcatccatgc cgtaaccgaa aacataacgg
     3061 ttcggcacat ccagtccgac ataatcggct cggataggct tgggtttgtc gatcagcttg
     3121 ttggcgaaca ccgccgcacg gcagcttgcc gcacccattt ccaaaagttt ggcttgaatg
     3181 gcggacatcg tatgcccttc gtccaaaata tcgtccagca cgacgacgtg cctgccccgg
     3241 atttgttccg catcgggcat acgcttccag ttgaacgcgc cgccctccag cttgtcgccg
     3301 taacgggaaa cgtgaacata atcaaaatct aagggaaaac gcaacagcgg cagcaactgc
     3361 cccgtaaaca ccaccgcgcc gcccatcacg ggcagcagca gcggatattt gccgcccaaa
     3421 tcgcgcgtaa tctcgtccgc cactttttgc agtgcggcac ggcattggcc ttggtcgaac
     3481 aaaagatcgg cgttttcaag catcgcctgt gtttcaaggc gtttggtttc taaatcggtc
     3541 atatgtcgga atcggtcggt aaaaggaaaa ttataaacca aagtatcgga tgccgtctga
     3601 actgtcctgc tcagcggtcg gtacgcacgc gcacaaacgt ggcgaatttc ggcgtgcctt
     3661 tctgcgtaaa gccacggtag cggtaggtaa tcagtgtgcc gattttgggc gggttgtctc
     3721 ggtctttatc tttgaagccg ctgccaatgc ggaattcgcc gtaccggttt ttgcagccga
     3781 ccgcgcccaa ccgtccggcg tttcgccctt tgccctcata gtgccgcgtt accgtgcatt
     3841 cgtcgtcgta ttggcttttc agcttcaata attggctgct cctgccgccg ctgtaacggg
     3901 attcgggctg acgcagcatc acgccttcgc cgccctgcgc ttcgatttgt tttaaaaagt
     3961 ccatcgcgtg ctgccggtcg cgcactttga tttgtgggat gatggtaatc ggcgcgttcg
     4021 gatgcgtttt cagccactgc gttgcgactg ccaaacgttg gtagagattg ccctgcgcct
     4081 tgggtacatc gaaaacgtgc aggcggatac cgcgccagtc tgaagaagca gaacgcacgg
     4141 tagcggaaat ctgctcgaac tgaccgcgtc cgctatacaa ttcgccgtcc aaaggataag
     4201 gcggaaactg cgcggtaaag cctttgggcg gtgtaaatac atatccttgc cggctgacta
     4261 aatgtttacc gtcccaatac gcgcgcacgc cgtcgagttt ctcgctcatc gcccagcctg
     4321 caatgtcctg ccctttgtat tcctgcgcca gcatcaaatc cgccgcgcct gctgatgcag
     4381 ggatgaaaac cgccgtaaaa atcggtatga tgccgccgat tgtcttctta atcatctgat
     4441 tcccccaata tcaaaacggg cggcaaaccg ccataaaaca aacggcaaac ccgatgccgt
     4501 ctgaaaaacc gtttaggaac acgccgatga ccctacgtta cgaaatcttc cccgttaccc
     4561 ccttccgcca aaactgcacc ctgatttggg acgacgaaag cggcgaagcc gtcctgaccg
     4621 atgtcggcgg cgacgtgccg ttcctgctgc aagcgttggc aaaccgcaaa cttacgctca
     4681 cggcaatctg gctgacgcac ggccatctcg atcacgcggg cggcgtggtc gaaatgttga
     4741 aaacgcataa agtccctgtc ctcgggccgc atccggacga tgaattcctg ctccaatcgc
     4801 tgccgcaaac caccgcgcaa tacggatttc ccgtctcgcc cgcctttgcg ccgaaccgtt
     4861 ggctcgaaga aggcgaaacg ctcacggtcg gacgctatgc ctttcaagtg ctgcatattc
     4921 cgggccatac gccgggacat atcgtctttt attgtgccga ggcggaattg ctgattgcgg
     4981 gcgacgtgct gttttacgaa accataggca gaaccgattt tccgcgcggc aaccacgccg
     5041 acttaatcaa taatatccgc aacaaattat tcacccttcc cgaaaccgtg caagttgtcg
     5101 ccggacacgg gcgtatgact tccatcggac acgaaaagcg gcacaatccg tttttctaac
     5161 cgccttccct acggtcttca gacggcatca tctgcactga tgccgtctga aacacaaaag
     5221 gctcagacaa ccgccgcctt gccggtgtac ctcgccagac aaggctttgg taagtacttc
     5281 aaacaaaccc aaaatcagaa ccagtacagt tacgccgcgc tttcggcatt cccgccccgg
     5341 ctgaaacaat atttttccgc acaagtcaga ctgcttcatc ttctgccgcg tattccaaag
     5401 attccgacaa cgccgttgtt tcattggaac gctcgaccaa atcccgttga agttgtttgc
     5461 ctgccttcac gcccaacaga cgcagtttct cggcgcgtcc gaccagattc ccgcgccctt
     5521 cggcaagttg cttgaatgcc gtctgaaaac tgctttgcgc ctgatcgatg cctttgccga
     5581 cgctttcgag cgtctgtacg aagccgacaa acttgtcgta cagcttgccg ccttcgtccg
     5641 caatcgccag tgcgttctga ttttgctgtt cgttgcgcca aatattcgcc accgtcctca
     5701 aagtcgccag cagcgtactg gggccgacca gcataatccg tttgtcgaaa cactcttgga
     5761 acaatcccgc gtcatgctgc aacgccagca ggtaggccgg ttcgacaggg ataaacataa
     5821 agacgaaatc caatgtgttc acaccttcca aatcggtgta atccttcagc gacaagcctt
     5881 tcatgtgtgc acggatgctg gcaacgtgtg ccgccagttc gcgtgccgcc gtatccgcat
     5941 ccgccgcctg cgtgtagcgc acataagctg tcagcgagac cttggagtca atcacaatct
     6001 gcttgttgtc gggcaggttg accaaaacgt cgggctggag gcggcgcgtg ccgccgtctt
     6061 cctcttttcg gacggatgcc gcctgaacca catattcgcg ccctttctgg aggccggaat
     6121 tttccaaaac cgtttccaga atcatctcgc cccaattacc ctgaaccttg ttctgcgtgc
     6181 cggtcagcgc gttggtcagg gcctttgcct cgctgtgcag ctgcgcattc aatccttgaa
     6241 gccgtttcaa ttcgttttcc aacgtcagcc gctcgcgcga ttctttatca taggtttgct
     6301 tgaccaactc gccgaaaccg tggatgcgtt cgtttagcgg gttcaaaacc tgatggagct
     6361 gctcgcggtt ctgctcggta aaacggcggc ttttttcttc caaaatcgtg ttggcaagat
     6421 tttgaaactg atcgctcaag ctcttgcgcg cctcgcccag taaggacagc ttctcttcag
     6481 aagcaaggcg ttcctgttcg atttgcgttg ccaaacgttc gttttcaacc gccaaaccct
     6541 gtgccttttc ctgcaactcg gtatgcgact gcctcaaccg ctccgcttcc gcctcttttt
     6601 cctgcaaatg ggcaatctgt ttttcggctg cggcaaaacg gttgccgaca tcggcaaggt
     6661 cgttttgcac gtcgcggaca gtttggcggc tttcttccaa atcggtttcg attctttggc
     6721 ggatttggcg ttccaaggca tattggtcgg caaacgcctt ccgttcctgt ccgagctgcg
     6781 ttgccaaacg ttcgttttca accgccaaac cctgtgcctt ttcctgcaac tcgatatacg
     6841 actgcttcag ccgaaccgat tccgcctctt tttcctgcaa atgggcaatc tgcttttcgg
     6901 ctgcggaaaa acggttgccc aaagcataat tttcgtcctg caaatgtcgg tatttcccgt
     6961 ccaaaacggc caattccgat gcaatttctg cgtgtgcctg ttcgacaaaa tcacatcttg
     7021 ccgccttttc cgccagttgc gcgttcaaat cggcaaactc gccctgaaac ctgcccttca
     7081 tcagcaacca tgtaaacaac acgcccgaca ccaacgccgc caaaggcagc aaaacagtca
     7141 tcagttccat caatcatcct aatattcaaa cattttcaca ccggacaaaa ccgccgctta
     7201 ttccgattct acctgtttgt tcgacatagc tccaaaaaat atagcggatt ggctttaaac
     7261 ctgttcgaca tcgccttacc atgctgcttg cggtttcaga ccttttccta attcaatatc
     7321 aatctgccac aaaccctgat taagttcccg atgtctgaca tttttagaat gatgccgtct
     7381 gaaatgttgc agctatgttc agacggcata cggatttagg cttttcaaac ggcaggcaaa
     7441 atgaaaaaag ggcaaaccct aaaggatttg cccttttgtt ccaaacgctt agtgtacgtc
     7501 tttccaatat tcttttttca ggaaatacgc caaaggcagc ataaccgcaa ataggaaaat
     7561 catcacgaca tagcctatac gtttgcgttg cagttgtgca ggttcgccca tgtacacaag
     7621 gtaattgacc aaatcgcgta catatgcgtc gtactctttt tggatcactt tgccgttagg
     7681 cagacggcgg ctgtgcaaac cggtagattc ccaatacagc ttaggcttca tctcgccgtg
     7741 ttcgtctttt accataaccg gctgaccttt ggcatccaac tcaacggctt gaacgccttg
     7801 ctgttcccac aacgggtggg gcataccgac tttatcgaat acagtattgt tccagccgct
     7861 cggacgggtc ggatctttat agaagccgcg catataagcg taaaggtagt ctgcaccttt
     7921 ggaacgcgca atcaatgtca aatcgggcgg agcagcacca aaccattttg ccgcatcttt
     7981 cgggttcatc gccgaatgca tgacatcgcc gacattatcg gtggtaaaca tcaggttttt
     8041 cttgatttct tcgtcagtca aaccgatgtc tttcaaacgg ttgaaacgca taccgcttgc
     8101 cgagtggcag gacaggcaat agttggtaaa gatttgtgcg ccgcgctgca ggctgacttg
     8161 gtcgcgcaaa tcgatatcga ctttctcata gtgtgcatga ccgcccgaag caaatgccgc
     8221 acccatgggt actgccaaca gcaaggcagc aaaccaattt ttcatagctt gtttcatttt
     8281 gactgccctc atcagatgtt ggttgcaaac aggtaagcac ccacggcggt aatgccgacg
     8341 taaacaaaga acatgatttt ttgtttggcg gtgctcatgg ttacgcgttc gggaaccggt
     8401 ttgtttttat ccagcttggt atagaacggc atacccagga agaatgcgaa atagacgaag
     8461 gacaggatgc gcgcgaccaa agtacgcgta tcggttgcca ccatcgcgcc caaaataccc
     8521 aaaccgataa aggaaatgat gaacaagacc aatgcggttt tgaaaatcgg accgcggtaa
     8581 cggacggatt tcacctcgcc cctatccagc caaggcagca gggcaatcaa cacgaccgcc
     8641 gcgcccatac cgattacacc ccaaacctgc gttcccaaga acgagggaat cgcacgcaaa
     8701 atggcgtaga acggtgtgaa ataccatacc ggcgcaatgt gcgggggtgt tttcagcgca
     8761 tttgccgcat cgaagttcgg cgcttcgagg aagtagccgc cgccttccgg tgcgaagaac
     8821 aatacggcac agaagacaat caggaataca accacaccca agatgtcttt aacggtatag
     8881 taaggatgga atgggatacc gtcgcgcggg ataccgtttt cgtctttgtt tttcttgatt
     8941 tcaacgccgt cagggttgtt agaacccact tcgtgcaggg cgatgatgtg cgccacaacc
     9001 aagccgagca ataccagagg taccgcgata acgtgcaggg caaagaaacg gttcagggta
     9061 acgtcggaaa cgttgaagtc gccgcgaatc caagtggaca aatccggacc gataacaggg
     9121 atggcggaga acaggttaat aattacctgc gccccccaaa aggacatctg accccaaggc
     9181 agcaggtagc ccataaaggc ttccgccatc aatgccaaga aaatcagaga accgaaaatc
     9241 cacaccaact cacggggttt tttgtacgaa ccgtaaatca aaccacggaa catgtgcaga
     9301 taaacaacaa tgaagaagaa agatgcgccg gtggaatgca tgtagcggat aatccaaccg
     9361 ccggacacgt cgcgcatgat gtactcgact gcagtaaagg cagcaggcag atggtaggcg
     9421 ttcaggttgc cgtcgggttt gtagttcatg gtcaggaaaa tgccgctgac gatttgaatc
     9481 acgaggacaa gcagggccaa agagccgaag tagtaccaaa aattaaagtt tttcggtgcg
     9541 tagtattgag ccaaatgctc attccacatt ttagacaggg ggaaacgagc gtccatccag
     9601 cctaacaatg cttttgcttt gctattggtt tggtttgcca taattatcgt tccttattct
     9661 tagtcttcgc ccaccaagat agttgtgtcg ctcaagtatt tatatggcgg gacaaccagg
     9721 ttggtcgggg caggaacacc tttatatacg cggccggcca agtcgaattt cgaaccgtgg
     9781 caggggcaga agaagccgcc tttccagtct gcacccaaat cggcgggggc aatgtcggga
     9841 cggaaggtgg gcgagcagcc caaatgggtg cagataccga tggcgacaag gatgttcggc
     9901 ttaatcgaac gggtctcgtt tttagcatac tccggctgct gttccgcatc ggaattggga
     9961 tcggtaagtt cgccgttcag gcctttcagg tctttaagct gctgatctgt acggttgagc
    10021 acccaaatcg gtttgccttg ccactcggcg gtcagcagct gacccgcttc gattttactg
    10081 acatccacct cgacggcagc accggcggcc ttggcttttt ccgaagggaa aaaactggcc
    10141 acaaacggcg ttgccacacc caatgctgcc actccgcccg cgccgcaggt cgcgagtgtc
    10201 aggaaacggc ggcggccgtt gttgatttct tgattatcca ttattcagtc gtcctaatat
    10261 tttgggaata ccgagccatt aaacgttgca attttaccca gtttgcagtg atactcaaag
    10321 cattatttaa aataaggtaa agttttatga tatttctcaa gactcaagcc ggattgtttt
    10381 cgtcaaaatg gcacacttcc aacccgaaaa cctctgccgc cgattctgcc agcgcgcgta
    10441 cgccgtaacg ttccgtcgcg tgatgccctg ccgaaatgaa agccgtaccc gtttcattgg
    10501 caaggtggta ttgggcttcg gaaatttccc ccgtcaaata cagatcgaca ccttcgtcta
    10561 ttgccgtctg aaaaaacccc tgcgccccgc cgctgcacca tgcaacccgt cggatttcgc
    10621 gttcgggttt gccgatgacg acaggcttac gttgcaaaac tgtttcaata tgcgccgcca
    10681 atgcgccgag tgtcttggct tgtttcaggc tgcccgagtt gagcaggttt tgttcgccga
    10741 accgtttttc tgtcgcaaaa cccaatctgt cggcgagttg ggcattgttg cccagtgtgg
    10801 gatgtgcatc caggggcaga tggtagcctg ccatattgat gtcgtgccgt aacagtgcgg
    10861 caatccgttc ttttttccaa ccagtaacgg tcggcaactc gtttttccag aacataccgt
    10921 gatgtaccaa aagcaaatct gccttctgct ccacagcaaa atcaatcgct gccctgcttg
    10981 ccgttaccga cgtaacgatt ttcccgatat attccctccc ttcaacctgc aaaccgttag
    11041 gggcgtaatc tttaaacaac gctgtctgca atgtttcatt acaccaagtc agaaaatccc
    11101 tgcgcaatac catctttttt cctaatcgct ttaaacaagc gggcattcta atcgcaaaat
    11161 gtctggaatt cacatttttc cgatttgcac ccgcatatga attattttaa tatgcgccgg
    11221 ttcaatatgc cgtctgaaac ctcatggatt ccatcatcga actgcgccac ctcaagaccc
    11281 tgctggcact tgaagaaacc ggcagcgtct cccttgccgc caaacgggtt ttccttaccc
    11341 aatctgcact ttcccaccaa atccgtatgc ttgaaaacca ctacggcgca ccgttgttcg
    11401 aacgaaaatc cacgcccttg cgctttaccc cggttggaga aaggctgtta cgcctcgctc
    11461 acgacctgtt gcctcaagtt accgatgcag aacgtgattt ggcacgcatt atagaaggag
    11521 aggcaggaga attgcggatt gccgtcgaat gccatacctg tttcgactgg ctcatgcccg
    11581 caatgggcga attccgcccg atatggcccc aaatcgaatt ggatatcgta tcgggattcc
    11641 aagcggaccc tatcggactg ctgctgcaac accgtgccga ccttgccatt gtttccgaag
    11701 cggaaaaaca aaacggtatt tctttccaac cgctgtttgc ctacgaaatg gtcggcattt
    11761 gcgcaccaga ccatccgctt gccgccaaaa ctgtctggga agccgaagac tttatcgggg
    11821 aaaccctgat tacttatccc gttcccgacg agatgctgga tttgcccaaa aaaatcctga
    11881 ttccgaaaaa catcaacccg ccgcgccgac acagcgagct gaccatcgcc attatccaac
    11941 tggttgccag cagacgtggc attgccgccc ttccctattg gacagtcatg ccctaccttg
    12001 aaaaaggcta tgtcgttcac cggcagatta ctgccgacgg attgcaaagc aaactgtatg
    12061 ccgccatccg taccgaagat acggacaaga gctatctgaa caatttttgc caaatcatac
    12121 gcgaacgcgg ttttgcagat ttgcccggac tgagcgaact ggaaccggtc tgacccctta
    12181 ttcaaccata cccggcagtt tttctatttt ttcatgtata gtggattaac aaaaaccagt
    12241 acagcgttgc ctcgccttgc catactattt gtactgtctg cggcttcgtc gccttgtcct
    12301 gatttttgtt aatccactat actgtttttg atttttgccc aatctgtaat ctttagattg
    12361 ccaatgggaa accgtctact acaaataaaa aaaccctgcg ataagcaggg ttttttgaat
    12421 ttccaacatt aacgtttgga gaattgtttt gcacggcgtg ctttgcgcag acccggtttt
    12481 ttacgttcga cttcgcgggc atcgcgggta acaaaaccgg cttgagacaa ggcgggtttc
    12541 aacgcggcat cgaagtcgat cagggcacgg gtaatgccgt ggcggattgc gccggactgg
    12601 ccggtttcgc cgccgccaac aacattgact ttgatgtcga aagattcggc gttttcagtc
    12661 agaaccaagg gttggcgaac aaccattcgg ctggtttcgc gtgcgaagaa ttcgtcaacg
    12721 ggacggccgt ttacgatgat ttgacctgta cctttaatca ggaatacacg agccactgaa
    12781 cttttgcggc ggcctgtgcc gtagtagtat ttaccgttca tgtcgcgtcc ttatttcagt
    12841 tccaaaactt tgggttgttg cgcagcatgg gcgtgttccg cacccgcata cactttcagt
    12901 tttttaatca tggcgtaacc caaaggacct ttgggcagca taccttttac agcttgttcc
    12961 aaagcgcggc ccgggaattg ctcttgcatt tcgcggaaag tgcgttcgta gataccgcct
    13021 gggaaaccgg aatggcggaa gtatttttta tcttcgaatt tggcaccggt tacacgcagt
    13081 ttgtccgcat tgataacaat gatgtaatcg ccggtatcga cgtggggggt gtattcaggt
    13141 ttgtgtttgc cacgcagacg gctggcgact tcggccgcaa cgcgacccaa gactttgtct
    13201 tgggcatcga tgacgaacca ttcgcgcttc acctcgtggg gtttcgctga aaaggttttc
    13261 atagtggaaa tccaaataga tatagaaagt tgtaaatttt aaagacagga ttcgattttg
    13321 tcaatcgcat taccgcgtta cggaaggatt ttccggattt cggcagactg catactgctt
    13381 tttcgggcgg ggcggcggcc aatgtgaaaa accgcatcgt tgcgatgcgg ttttgaatgg
    13441 gaatccccgc gagagccgtt tcggccgaat ccgcttgaac cttgctgaca aggcggctgc
    13501 ctcgggtagt ttcgggtgcg tccgcaaaag gacgctcgcg cccactactg ctcccggcaa
    13561 ccttaagcga acttattggt tcaaaggaat atatgccttc gcggacaccg cagggaaaaa
    13621 ggggttattc ctgcgccaag cgggatagtg ctttttggca ggcgttgtcc atatcggcta
    13681 ttttacgcgc aaaatcgccg attgccaaat cgccgccgtt cagggaggtt ttcaacaggt
    13741 cgtggacgac gttgagcgcg gccataatga cgattttttc gctgtccgcg acgcgtccgc
    13801 cttcgcggat ggcttcggct ttgccgttga gcattccgac tgcctgcaac agtgtgtctt
    13861 tttcttctgc cggcgtgttg acggtcagcc gggcgtgcat gacttcgatg tggacttgtt
    13921 cgatgttcat cctttaatcc ttattgctgc gtttcctgcc attgggggag gcgcgctgcc
    13981 agtgcgctga ttttttccct gctctgttcg agcaggctgc ggtatcgtgt attttcttct
    14041 gtcaggctgt cgattttgtt ttgcaggtct tctttgagtt tgccgacttg gacgagcagg
    14101 gcttcgctga gttcgtcgac ggcggtttcg tgttcgagtt tttgccgctc gtgcgcccgt
    14161 ttgagttcgg cgacggtttc tttgaggcgg cggttttcgc tgacgagggt ttcgaatttt
    14221 tgtaccagcg tataaacgct gctttcgagt ttttcgatat tttgtttcat aaccttacct
    14281 gtccgtatgc cgtctgaagg cttcagacgg catctgtctg ttgtttattc aaaacgcgcg
    14341 ctgcgttcca tcagcctttc gacgacctgt tgcggggtca tttctttgcg gatgagttgc
    14401 agcagggttt gggtaatcgg catgtcgatt tggtacttac aggcagtatt gaagacttct
    14461 tctatcgtgc tgaccccttc ggaaacgtgt ccgatttcga ccagcacctg atgcagttcc
    14521 ttgccttctg ccaaacccaa gccgacgcgg cggttgcgtg agagtgcgcc ggtgcaggtc
    14581 aagatgaggt cgccgatgcc tgccagcccc atcatggttt tgggctgtgc gcccattgcg
    14641 gaggcaaggc gggtgatttc agctaatccg cgcgtaacca gtgcggcgcg ggcgttaagc
    14701 ccgtactcta gaccgtcgga caatccggtg gcaatcgcca taacattttt taccgcgccg
    14761 ccgactgcca cgccgataac atcggtactg ccgtaaagcc tcatgacggt cgtgttgagc
    14821 tgcggtacga gttcttcaat ccactcttgg ttttcggagg cgaggacgac ggcgcagggc
    14881 agttgtttgg cgagttcctg tgcaaaactc gggccggaga gtacgccgat tttcttattg
    14941 tcgggcaata cttctttcaa tacttgaaag gtcagcagcc ctgtatcctg ctcgaatcct
    15001 ttgcaggcgg cgaggacggg gaggtgtccc gcgccgtact gtttgagcag ctctgcgctg
    15061 cttctcaatc cggcaacgga ggttacgata aggacaagtc cgctgtcttt gagcgcgtct
    15121 gccaaatccg cacacacttc caaggtttcg ggaaaggaaa agccgggcag tccgcgtttg
    15181 ttttcacgcg cttcctgcat ttggcggact tggtctgcgt tgcgcgtcca cagggatacg
    15241 cggttgccgt gttgggaaaa gtgcagggcg agcgccgtac cccacgaacc tgcgccgata
    15301 acggtaattt tcattggtcg tctttcaaca tatcactgcc gttcacttta aaacaatcgg
    15361 tgtttctctg caagtgcggt cagggaaatg ccgtctgaaa ggtgttcaga cggcattttg
    15421 ccccgatgcg gcacaatcag cctgtattgc gcaaaccttg cgccacgccg ttgatggtca
    15481 ggtgaaccat cagcagcgcg tgcggattgt cgggttcttt acgcaggcgt ttgagcatgg
    15541 cgacctgcag gccgttgagc gcgttcaaat atggaatcct caaggcaagc gaacgggcga
    15601 ggctgcggtt gtcgcgtaaa agttcttcgg tttgcagcag gtcgagcagg gctttgcggc
    15661 tgcgttggta ttcttcctta atcatgccga agatgacggc ggctttttcc ggcgattcgc
    15721 tcaagccggc atagttttcc gcgagggtga tgtcggtttt cgccatcact tgctccatat
    15781 tggaaagcat ggcttggaag aacgggttgc tttgggcgtg ttcgcgcagg gcggcgagcg
    15841 tttcgggttt gtcttcgcac aaggtttcca ccgcgctgcc gaaaccgtac caagcaggca
    15901 gcatgaggcg gttttgcatc catgagaata cccacggaat agcgcgcaag tcctgaatcc
    15961 gcgccaaggt tttgcggctg gcaggacggc tgccgaggtt gagggtggcg atttcctgaa
    16021 tcgggctggt ttgcaggaag tagtcgatga agtcggggtg ggtaatcagt tcgcggtagt
    16081 atttgaacga tacgtccgac aatgcctgca tcagtttggc atcagggtct tttttatccg
    16141 gcaggatgct ggcttccaaa gtcgcggcaa ccaaggtttc caagttgcgt tgggcattgc
    16201 cggggtcggc gtatttggcg gtaatgactt cgccttgttc ggtaatgcgg atttgtcccg
    16261 ccacgctgcc cgccggttgg gcgagaatgg cttggtaaga agggccgccg ccgcgtccga
    16321 cgctgccgcc gcgaccgtgg aacaggcgca tgcggacatc gtattttttg aagagttcga
    16381 ccaagcccaa ttccgcctga tacagacacc atgagctggt aacatagccg ccgtctttgt
    16441 tggagtcgga gtagccgagc ataatttctt ggatgttgcc gcagctttcg agcagggcat
    16501 cgtaccaatc gaggcggaac atggtttcca tgaccggaca ggcgttttcg agtgcttcga
    16561 tggtttcaaa cagcggcacg atattgatgc ggctgcgcgg tttgccgttt tccaccgcca
    16621 gcaggccgct ttctttcagc agcaatgcca aggcgagcag gtcgctgggt tgttcgcagt
    16681 tggaaataat gctttgtgta acggcatctt cgccgaattc gtctttgatt ttgcgcgctt
    16741 cgttgaaaat tgccagttcg tggcgggtat ggtcgctgta tgtgatgaac gggctgtaca
    16801 gcggacgttg atggctcaat tcgcgcaaca gggcggcttg tttttgctct tcgttcaggc
    16861 tgttgtagtc ttccaagcct gcgtgttgga aaagctcggc aaccacatcg gcgtgtttgc
    16921 ctgcgtgttg gcgcaggtcg agcggcatca tgtggaagcc gaacacggac acggaacgga
    16981 tgaggtctgc caaacggcct tcggcaagca ggcggctgcc gttgtcgata agggaacgtt
    17041 gcaatttttt caaatcatcg agaaattctt gtgccgaagc ataaggctcg agaaagccga
    17101 atttgcagcc catgcccaaa ccgagcgcgc gcgctttgcc catagcgcgc gccataatgt
    17161 aggcaatggc gcggcggtag ggttcttccg tgcgggcaag ttcttcgtca ggcgagaggg
    17221 ctgccagtgc cattacgtcg tcgttgactt tgacgcggcg gatggagagc ggcaattcgc
    17281 ggtagagttt gtcgagttcg ccacggtaaa aacggaacac ggcatcggcg tggcggcgga
    17341 aggcaaagcg cagggtttcg gcagaaacga acggattgcc gtcgcggtcg ccaccgatcc
    17401 agccgccgat tttgaggatg ttcggaacgc ggacatcggg ataggccgtc tgaaagtcgt
    17461 gttccatctt gcggtagagt ttgggcaggg cttcaaaaaa gctcatcggg aagatggaca
    17521 cgccgttgtt gatttcgtcg ttgacgctga gtttgtggcg gcgcgtttcg ctggtctgcc
    17581 acaaacccag caggatagtg tcgatttcgc ggcgcagccg tgccagcgcg tcggcattgg
    17641 tgcagcgttc gcgttgcggc agcagcgcgc ggatgcggcg gttgaaattc aaaacggttt
    17701 ggcgttgcac ttcggtcggg tgcgcggtca aaacggcggt aacggacgta ttgtccaact
    17761 gccgctgcac cgatttgccg tcggctttcc ccgctttgag cctgcggacg gtttccgtca
    17821 ggctgccttc cgcgccgccg cgtccggctt cttcgtggat ttggcggcgg cgttcgtggt
    17881 gcacgtcttc ggcgatattc aaaatctggg cgaacaggcc gcaggccaag gttaaatcgt
    17941 gggtttgttg ttcgtccaat tgcggcaata ctttttcaat caatgccgcg ctgtcgtcgg
    18001 aagtggacaa gagtttgacc gtttcgacaa ccaatggcga ggcttcttcg tgcaggaggt
    18061 tgaacaggga ttgtttcaga aattccgcgt ccgccgccaa agccgcgtcc tttggattgt
    18121 tcagaatatg cagttgcatg atttttctct cattgccgta aatactgtaa atgtacctca
    18181 aatgccgcat ccgtgccaaa ccgttcacac tttaaccgcc cgtgtcccga aatgccgtct
    18241 gaagttgaac gccgcccgac ggcagcgtta caatcgcccg caactgtttt ttccgaacat
    18301 catcatgacc acgaccgaac acgacaacga cgatgcattc ctgctgcggt acagccgcca
    18361 catcctcttg gacgaaattg gcatcgaagg gcagcagaaa ctttccgccg cgcatatttt
    18421 ggtcgtcggc tgcggcggtt tgggtgccgc cgccctgccc tatctcgccg cttccggcat
    18481 cggcacgctg accatagccg attccgacac ggtcgagctg cacaacctgc aacgccaagt
    18541 cgcatttgac gagggcgatg tcggcaagcc caaagccgaa gccttggcag accgcctgaa
    18601 acgcatcaac cataacgtcg atgtccgcac cgtcaacgaa aaactcgacg gctgccgcct
    18661 gaccggtttg gttcaaaccg ccgacatcgt tttagactgc tgcgacaact acgccacgcg
    18721 ccaagccgtc aaccgtgtct gcgtacaaac gaaaacaccg ctggtttcag gagcagcggt
    18781 gcgctttgaa gggcaacttg ccgtgtaccg tcccgacttg cccgactcgc cgtgttacgc
    18841 ctgcctgttt gacggcggat cggcttcaga cggcatctgt tccctcttcg gcgtattctc
    18901 gccgctggtc ggcatcatcg gctgcacgca ggcggcggag gctctgaaaa tcctgctaga
    18961 cgtgggcgaa ccgtcgcacg gcaggctggc ggtttaccgt gccttggaag ggggctggca
    19021 atatttcgac ctgccgcgca accccgaatg cccgatttgc ggcacggaac ggtaaaaccc
    19081 tgccgccgtt tcagacggca taaagacgga tgcggaggaa cggttttaaa aatcggcggt
    19141 gttttgacat agtgtcgata agcatactta tcttcaatat actcaacagg ataattgttt
    19201 gcctaagcaa aaaataatcg ccttttccct gacgggcggg cgaaatccac ggattgcaaa
    19261 aacacaacct gaaaatttac atttcttt
//
LOCUS       DAXHKX010000022        18232 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHKX010000022 DAXHKX010000000
VERSION     DAXHKX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.022"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="ACFMWZ_000644"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="ACFMWZ_000644"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC5934640.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDVYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMINTKTVAVEPKEDGVYVTFEGTNAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVVVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGHIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1965..3572)
                     /gene="aceF"
                     /locus_tag="ACFMWZ_000645"
     CDS             complement(1965..3572)
                     /gene="aceF"
                     /locus_tag="ACFMWZ_000645"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC5934641.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGATAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGTAIIEVETAGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSSLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEETDMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPIWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3722..6385)
                     /gene="aceE"
                     /locus_tag="ACFMWZ_000646"
     CDS             complement(3722..6385)
                     /gene="aceE"
                     /locus_tag="ACFMWZ_000646"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC5934642.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7080..8792
                     /locus_tag="ACFMWZ_000647"
     CDS             7080..8792
                     /locus_tag="ACFMWZ_000647"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC5934643.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFKGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            8855..9511
                     /locus_tag="ACFMWZ_000648"
     CDS             8855..9511
                     /locus_tag="ACFMWZ_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC5934644.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9521..9976)
                     /gene="ruvX"
                     /locus_tag="ACFMWZ_000649"
     CDS             complement(9521..9976)
                     /gene="ruvX"
                     /locus_tag="ACFMWZ_000649"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC5934645.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9969..10517)
                     /locus_tag="ACFMWZ_000650"
     CDS             complement(9969..10517)
                     /locus_tag="ACFMWZ_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC5934646.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGHWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10519..11079)
                     /locus_tag="ACFMWZ_000651"
     CDS             complement(10519..11079)
                     /locus_tag="ACFMWZ_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC5934647.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMINLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11407..13212
                     /locus_tag="ACFMWZ_000652"
     CDS             11407..13212
                     /locus_tag="ACFMWZ_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC5934648.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNAELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13332..14807
                     /locus_tag="ACFMWZ_000653"
     CDS             13332..14807
                     /locus_tag="ACFMWZ_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC5934649.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAEQAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGREG
                     MEGFVLKARPEDYIFKAGADPLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNGETLVVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
     gene            15136..17163
                     /gene="uvrB"
                     /locus_tag="ACFMWZ_000654"
     CDS             15136..17163
                     /gene="uvrB"
                     /locus_tag="ACFMWZ_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC5934650.1"
                     /translation="MEVIQYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRIGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQMKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            17231..17995
                     /locus_tag="ACFMWZ_000655"
     CDS             17231..17995
                     /locus_tag="ACFMWZ_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFC5934651.1"
                     /translation="MAEQLQLIPRVESGEIIPQRMSDGYINATALCKSVGKSYSDYRQ
                     LQSTNHFLNELKAQTGLSEQQLIQQRIGGEPSLQGSWVHPYLAINLAQWLSPAFAVKV
                     STWVHEWMSGKVNASPSHSAHIQRYLQNRGKIPYTHFSMLNEITLNLIAPLEEQGYVL
                     PSQLVPDISEGRLFCRWLRENRNVDPNNFPTYKHEFLDARRPVEAKLYPIEYYQDFKW
                     HFNEVWLKEKAKQYFSERDAAILPYLTPLMLPASES"
     gene            complement(18094..18232)
                     /locus_tag="ACFMWZ_000656"
                     /pseudo
     CDS             complement(18094..18232)
                     /locus_tag="ACFMWZ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         4502 a         4585 c         4930 g         4215 t
ORIGIN      
        1 tcggagaaga tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 cattttctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgcagc cataccgatg gattcgccaa
      181 gggttgggtg tgggtggatg gttttaccga tatcggctgc atcacagccc atttcgatgg
      241 ccaagcagac ttcgccgatc atatcgccgc cgttgggtcc gacgataccg ccaccgatga
      301 tgtggccggt ttcagcatca aaaatcagct tagtgaaacc attgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagcagcc catgggaagt tggctttggt gattttgcgg ccggaggctt
      421 tggcagataa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacattg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacga
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttcg gtgcgttcgt gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gatcatgatg ttgtcaaaac
      841 gatattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaacgacg tcaaggcgtg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagtgcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggtaacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgcctgttc gtacacatcg ccggcagtca gcgacacttc caagtggtgt ggatctaaga
     1201 attgcccgtc gccttggata acgtccactt tacggctttt cgccatacct gccaaaccgc
     1261 ccgtgaggcg ggaaactacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg accgccaccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ccgcttcggc tttaggagcg gcagccgtgc cttcagcttc aacgacaaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt ttacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcacc cacgttcact tcaaccgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aagctcatgt ctcatctttc tgaatgatgt tcaaacttcc
     1921 ttttcagacg gcacagaccg ctatgccgtc tgaagccgat cgggttacag ggtaatgcgg
     1981 cggaagtctt tcaacagctt cgccaagaat acggtgaagc gcataccggc cgcaccgtcg
     2041 atgacgcggt ggtcgaagga caggctcaac gggcacatca ggcgcggggc aaactctttg
     2101 ccgttccaaa tcggtttgat ttgggatttg cacacgccca agatggcgac ttcgggagcg
     2161 tttacaatcg gcgtgaagcc tgtgccgccg ataccgccca agctggaaat ggtaaagcac
     2221 gcgccttgca tttcttgcgg tttgagcttg ccttcgcggg cttttttgga caattcggtc
     2281 agctcttggc tgatttgttt caagcctttt tgatccacgt ctttgatgac gggaacaacc
     2341 aagccgttcg gcgtatcggc tgcgaaaccg atgttgaagt agtttttcag caccaggttg
     2401 tcgccgtcca gagaagcgtt gaattcgggg aaggctttca gcgcggaaac ggaggctttg
     2461 atgatgaacg ccaacggtga cagtttcacg ccttcgcgtt cccattcttt gttcagttgt
     2521 ttgcggaatt cttccagctc ggtcatatct gtttcttcgt gtacggtaac gtggggaatc
     2581 accacccagt tgcgagacag gttttgaccg gagattttct taatgcggga caattcttta
     2641 acttcgacat tgccgaattt ggagaagtcc actttaggcc acggcagcaa gtccagactg
     2701 ctgcccaaag atgcgccggc tgcggcaggt tttgccgcgc caccctgcat tacggatttc
     2761 acaaaggctt tgatgtcgtc gcccacgata cggcctttca agccggtacc tttgacttgg
     2821 cccaaatcca cgcccaattc gcgcgccagt ttgcgtgcgg aagggccggc gtgtgctttg
     2881 gcgaaagcgg cctcgtcgat tttggcagcg gcaggtgcag aaggtgcagg cgcggcggcg
     2941 ggtgcggcgg cagcagtcgg aggcggagcg gcagccggtg cggcagcttg agcggcttga
     3001 gcaggggctg ctgcggcaga gccggcggtt tctacttcga taatggcagt accttcggac
     3061 actttgtcgc cgacttttaa gaatacggct ttaacgacac cggcagcggt acaaggtacg
     3121 tccatcgttg ctttgtcggt ttccaaagta atcagcgtgt cgtcttcggc aacggtatcg
     3181 ccgactttga tctcgacggc gattacatcc acatcggtat ggccgccgat atcgggaacg
     3241 gctacttgaa cggttgcacc gcctgcggca acgggtgcgg cagcaggtgc aggttgtgct
     3301 tcggcagcag caggagcggc ttcggcagcg gtggcaccgg tttcaacggt caaaattacg
     3361 ccgccttcgg agattttgtc gccgactttg acttttactt ctttcacgac accggccgca
     3421 tcggcaggca catccatcgt ggctttgtcg gtttccagtg taatcagggt gtcgtcaacg
     3481 gcgatggtgt cgcccgcttt aacttctacg gcgatgatgt cgacgttttc gtgaccgccg
     3541 atatcgggga ctttgatttc tacgatactc atttgagttc ctttaatctt tttcaattgg
     3601 atgccgtctg aaagctgctt caggcggcat cggtacggtt tgtctgctca aaccattcgg
     3661 gcattcggtt ttcagacggc ctgatgtcgg aatggggcaa acaggccgtc tgaaacatca
     3721 atcagcgttt ccagctagga gctgaatcgg ctttgatgcc gtatttctca atggcttgtt
     3781 gaacggtttc tttgctgact ttgccttgtt ccgccaatgc ggccaatgcg gccacggcaa
     3841 cgttgtagcg atccacttca aagaagcggc gcaggttggc gcgactgtcg gaacggccga
     3901 aaccgtcagt gcccaagacg tggtagtcgt tcgggatgta ggcgcggata cggtcagcat
     3961 agctgcggat atagtcggta gcggcaatca ccggaccgtc atgaccttgc agttgggaag
     4021 taacgaaagg tactttttcg gcttccagcg gatgcaggcg gttgaagcgt tctacctcga
     4081 cagcgtcgcg gtgcagcagg ttgaaggacg ggcaagacca gatgtctgct tctacgccga
     4141 agtcggcttt cagcagctcg gcaccggcaa tgacttcttg caggatggta ccggagccca
     4201 tcaattgaac tttcttatcg cctttgccgc cggctttcag cagatacata cctttcagga
     4261 tgtcttgttc cgcaccttcg ggcatatccg gatgggtgta gttctcgttc atcagggtga
     4321 tgtagtagaa cacgtcttca ttattggcat acatacggcg cagaccgtct tgtacgatga
     4381 cggcgacttc gtattggaaa gtcgggtcat aagatacgca gttcggaatc aggtcggcct
     4441 ggatgtggct gtggccgtct tcgtgttgca ggccttcgcc gttcagcgtc gtacggccgg
     4501 cagtaccgcc cagcaggaag ccgcgcgcgt gcatatcgcc cgccgcccaa gccaagtcgc
     4561 cgatacgttg gaaaccgaac atagaatagt aaatgtagaa cggaatcatg gcgaagttgc
     4621 tgttggcgta gctggttgca gccgcaatcc agtcggccat cgcgcccggt tcgttaatac
     4681 cttcttgcaa gatttgaccg tcaacggatt ctttatagaa catcagttgg tctttgtctt
     4741 gaggggtata ttgctgacct ttcggattcc aaataccgta ttggcggaac ataccttcca
     4801 tgccgaaagt acggctttcg tcgggaacga taggtacgac gcgtttgccg atttttttgt
     4861 ctttcagtaa agtggacagg atgcggacga atgccatcgt ggtcgagaac tcgcgttcac
     4921 cgctggattt gagttgtgcg tcgaatgctg acagctcggg cacttccaat acttcctgcg
     4981 tcggtttgcg ttgcggcagg tagccgccca aagcatcgcg gcgtgcgtgc aggtatttgt
     5041 attcttccgt atcgggggca aaagtcaggt aaggcagatc gccgctttcg atttgttcgt
     5101 cggtaaccgg aatgtcaaag cggtcgcgga attgtttcag ggacgctttg tccatttttt
     5161 tggcttggtg ggcaacgttc tgaccttcgc cggatgcgcc cataccgtaa cctttaatgg
     5221 ttttcgccaa gatgacggta ggtttgccgt cggcatggtt cgctgcgcgg tcgtaggcgt
     5281 tgtacacttt ttgcgggtcg tggccgccgc ggttcaatgc ccagagttgc tcatcggtca
     5341 tatcggcaac caatgctttc agttcgggcg tattgaagaa gtgttcgcgc acatacgcgc
     5401 cgtctttgga tttgtaagtt tggtagtcgc cgtccaaaca ttcttccata cgttggcgca
     5461 ggataccgtc tttgtctttc gccaagaggc ggtcccagcg gcggccccaa atgactttga
     5521 cgacattcca gccggcgccg gcaaagttgc cttccaattc ttggatgatt ttgccgttgc
     5581 cgcgtaccgg accgtccaag cgttgcagat tgcagttgat gacgaaaatc aggttgtcca
     5641 agccttcgcg tgcagccagt gcgattgcac cttgagattc gggttcgtcc atttcgccgt
     5701 cgccgcagaa acaccatact ttacggcctt tggtttttgc caaaccacgc gattccaagt
     5761 atttcaggaa acgcgcctga taaatcgcca tgatgggccc caagcccatg gatacggtcg
     5821 ggaactgcca aaagtcgggc aagaggtggg ggtgcggata ggaaggcaga ccgtgtccgt
     5881 ccacttcttg gcggaagttg ttcagctggt cttcggtcag acggccctcg acgaatgcgc
     5941 gtgcatagat gcccggagcg acgtgacctt ggaagaagac caaatcgcct tcttcgcctt
     6001 cgcctttggc tttccaaaag tggttgaaac cgacttcgta catggtggcg gcagattgga
     6061 aagatgcgat gtgcccaccc agttccaaat ctttcttgcc ggcgcgcaat acgatggcgg
     6121 cggcgttcca gcgcacgaat gcgcggatgc ggtgttcgat gttttggtcg cccggaatgc
     6181 ctttttcgtt ttcaaccgaa acggtattca aatacggggt ggtcgtgccg tgtggcatac
     6241 gtacgccctt gtcgcggcag tatttgacca ggttttccaa gaggtattgc gcgcgttcgc
     6301 cgccttcata ttcgaggacg gagcttaacg cgtccagcca ctcttgggtt tcgatagggt
     6361 caacatcgtg taattgggtg gacatagtat ctatccttta tgttgagtgt ttaatcggca
     6421 gggagggtgt agccaacccc tttattttcg tttgtgaaaa ataataagca aaaacgaaaa
     6481 tttctaaatc aaaccgaaaa aaaataattt aagtttaatg ttttcaaaaa attataaaag
     6541 ttttggaaac aagggaaaac ttgaaaaaaa tgttcgatct gctccacacg aaatcctatc
     6601 agcaatcggg tctgctggca aataaccatg cctgtaaaag caggttttat actgaagttg
     6661 gcgtttggta aggtcaagtc tttttgattt cgaaatattc tgtttgattt tggcgatatt
     6721 ttttcggaat tgtggtatat cgaatcatta tgtctctgtc ggcaattttc ctattgtaga
     6781 aaatctatac acaagctttg tggataaccg tgttgataag ccacggcttg tgttaaaaaa
     6841 gtcttacgca acaaatgtac gcttattttg attaaaaaat aggcacattt aaatctataa
     6901 aaatcatgtg cttatgttca atatgatgtt tctggtcatg tttttgccgt ctgttatggg
     6961 atttgtcccg cgcttggggc agggggtgaa ttttggaggt tggtgcaaaa cctgcttttt
     7021 tcgcgttaca atctgcaccc gatttacttt tgaatttgcc tgtttggaaa aatactttta
     7081 tgaaagccag ccaattcttt atctctactt taaaagaagc ccctgccgaa gccgcgcttg
     7141 ccagccacaa gctgatgatt cgcgccggcc tgattaaagc caacgcgtcc ggcctttata
     7201 cttggatgcc gatggggctg cgcgtgttac gcaaagtcga aaacgtcgtg cgcgaggaaa
     7261 tggcgcgcgc gggcagcgtg gagctgctga tgccggtggt gcagcctgcc gagctgtggc
     7321 aggaatccgg ccgctgggaa ttttacggta aagaactgct gcgcctgaaa gaccgccacg
     7381 accgcgattt ctgcatgggc ccgacctgcg aggaagtcat cgccgacatc gtgcgcaaag
     7441 aaatcaacag ctacaaacag ctgccgaaaa acttttacca catccaaacc aaattccgcg
     7501 acgaagtgcg cccgcgtttc ggcgtgatgc gcgcgcgcga gttcgtcatg aaagacgctt
     7561 attccttcca tgccgactac gcctcgcttc agacgaccta tgatgccatg tatgacgctt
     7621 actgccgtat cttcacccgt ctgggcttgg cattccgccc cgtcgccgca gacaccggca
     7681 gcatcggcgg tacgggttcg cacgagtttc aagtgttggc ggaaagcggc gaagatgtta
     7741 ttgcgtacag cgacacttcc gattacgccg ccaatatcga gttggcaccg accttgccgc
     7801 ttaaaggcga acgcgccgct gctcaggctg tgttgaccaa agtacataca ccaaacgtca
     7861 aaaccattga gtctttggtt gaattcctga atattccggt tgaacaaacc ctcaaatcca
     7921 tcgtggtcga aggcgaaaac gaaggcgaaa tcgtcttatt actgttgcgc ggcgaccatg
     7981 agttcaacga catcaaggca gaaaaactgg cgggcgtgaa atcgccattg acgatggcaa
     8041 gccctgccgc gattgtcgaa cagttcggcg caaacggcgg ctctcttggt cctgtcggct
     8101 tcaaaggcaa agtctatgcc gatttcgcta ccgaaaaagg cgcggactgg gtcatcggtg
     8161 cgaacgaaga cggctaccac tataccggct tcaacttcgg tcgcgatgcc gccgagcctg
     8221 agtttgtcga tttgcgcaac gtcgtcgaag gcgacgaaag ccccgacgga caaggccgtc
     8281 tgaaactggc gcgcggcatt gaagtcggac acgtcttcca gttgcgcgac aaatatacta
     8341 aagccatgaa cgtcagcttc ctcgacaaca acggcaaatc gcaaattatg gaaatgggct
     8401 gctacggcat cggcatcacc cgcgtcgttg ccgccgccat cgagcagaat aacgacgaaa
     8461 aaggcatcat ctggaccaaa gcaatggcgc cgtttgaagt cgttatcgtg ccgatgaact
     8521 acaaaaaatc agacaccgta cgcgaagccg ccgacaaaat ctatgccgaa ttgctggcgg
     8581 caggcgcgga tgtgctgctg gatgaccgcg acgaacgcgc aggcgtattg ctgaacgatt
     8641 ccgagctgtt gggtattccg caccgcatcg tcatcggcga ccgcgctttg aaagaaggca
     8701 atgtcgaata cgccgaacgc cgcaacaacg aagcgcaggc agttgcaatc ggagaaattg
     8761 ttgcgcgtgt aacagcttca ttaaatgggt aagcttggtc aaatgccgtc tgaaaataat
     8821 ttcagacggc atttttattt ggggagaaag tgaagtggct tcagtatttt tagaaggaga
     8881 agcagtcgag gtcggcaata ttttctgtat cggcaggaac tatgccgcac atattgaaga
     8941 gttaaaaaac gaaatacctt ccgaacctgt ggtgtttatg aagccgtcag gcagcatttt
     9001 gaatagcggc ggtacaatct tgctgcccga gtttagtagg gatgtgcagt ttgagtgcga
     9061 actcgtgctg ctggtcggta aggattcaga cggcacgggc gagggtaaag atattttggg
     9121 atgtgtcgca ggatatggtg tagggctgga ccttacggca cgggatatcc aatgccgtct
     9181 gaaggaaaaa gggctgccct ggctgaaggc aaaagggttc aggcattccg cgtgcgtgtc
     9241 ggattttgcg gcggcaggca gaatcggcaa tcccgagaat gtgttgtttt cattaaaaca
     9301 aaacggcgta ttgaaacagc gcggggatac cggtttgatg atttacccga ttcgggaaat
     9361 tttacacaaa ctggcggcag attacggttt ggggaaaggg gatttggtgt ttaccggaac
     9421 gccctccggc gtgggcgcaa tcggtgcggg ggacaatttg gcattggagc tggacggctt
     9481 ggtgcgcgcc tctttcacgg tagggtgtta accgcgccgc ttaaccctca cgcccgttga
     9541 aacattccgc cggaccgccc tcgaaaaaac cgtgcaagat ggcttgcgcc gccacttggt
     9601 cgagcaccga tttgcgtttt ttgccgaaga cctgtgcttc cgaaagcagg ctttcggcat
     9661 agacggacga cagccgttcg tcaacccaat agacggggag attgaacctg ccgttcagcc
     9721 tgcgtccgaa cttgcgcgac aggtgcgtca tttcatgttt cgtgccgtcg gtatgcacgg
     9781 gcagtccgac gacaaaataa cgcggctgcc attcttgaac cagcttggcg attgccgcga
     9841 acttttcatc gttgctgccg ccggtaacgg tgctcaaagg atgggatagc cctaattccg
     9901 cgtctccttg tgccacgccg atacgcgctt cgccgaagtc gaatgccagt gccgttcctt
     9961 ttggaatttt atgcatggcc ggctcccgaa aacagggcga gcgggtcgat gccgagtttg
    10021 gcgaatgcgg cggcgtaacg gtgttcgtag gggatgtcga acaggatgtg ttcgtcggcg
    10081 ggaacagtca gccacgcatt gtcggcaagt tcgcgttcga gctggccttt tccccagctt
    10141 gaatagccta tgctgatcaa ggctttgtca accgcacctt cgcgtgaaat attttcaatc
    10201 acgtctcggg aagaagttag cgcgatattg tctgaaacgc cgatactgct ttgccagtgg
    10261 ccgatcgggg tatgcacgac ataacctcgc tcgacttgca ccggcccgcc catcatcacg
    10321 ctgtcgtgct gcatccgcat ggggatgttt ttgccggtgg cggaaaaaat catgtccatc
    10381 gtaatcggag agggtttgtt gatggcgatg ccgagtgcgc cgtcttcatc gtgtttgcag
    10441 atatagacga ccgattgtga aaaaaacgcg tcttccatat cgggcatggc gacgagaaag
    10501 tggttggaga ggttcatatc agcgtttgtc gggattttct atcgggttgg aaatcatgca
    10561 ggcgccggca agcaggttgc cggaagtatc ggcttgcacc ccatcggttt gcggtatgcc
    10621 gccgccgaaa caggaaaccg cgcttaagga atttttcggc gagccttgga tgattttatc
    10681 gctgtacacc aaataggcga aggttttgcg tttggggtcg taataacgga caatctgccg
    10741 gctcttgaac gcgaagctcg caccgtgttt gaaaacttct ttcggtttgc gcacggcggt
    10801 ttcgtcaaaa gaaatcgaag atgccgtctg aacgcacgaa accgatgcgt cggacgcatc
    10861 ctcttccaaa ttgatcattt ccttcaagcc gccttttttt gcatacgaaa tataacaggc
    10921 aaccccttga acgtcgggat cgtcgaatcc ttccacttcg atacggtcgt ttttgcccag
    10981 catgttgaaa acggtacttg cccgtccgat tttatcggtt tcgccgctgc cgcaggcagt
    11041 cagcaggacg gcggcagaca gcagtagcag tctgttcatc aaaatttcct tatcggttga
    11101 atccccgttt tccgtgaagg cggcagggct tgctttgttt ggaggggcgc acgttcccaa
    11161 aatcgggcaa catacggtat gcggcttaat gtgctaccct gtgtgtcgga acacaatatc
    11221 cgttgcggca ttgaaaatat gacgcacaat ataaggctgt ttgagccgat tgcaagtatc
    11281 cgcgcaaaga aggcgcaaga ttgggcaaac ggcattttca gacggcataa ctgacagtat
    11341 aatccaaacg tccggctgct ttcgtgcagc cttcagtatt atccaacttt ttccgaaggt
    11401 cgaaccatgc gctacaaacc ccttctgctt gccctgatgc tcgttttttc cacgcccgcc
    11461 gttgccgccc acgacgcggc acacaaccgt tccgctgaag tgaaaaaaca gacgaagaac
    11521 aaaaaagaac agcccgaagc ggcggaaggc aaaaaagaaa aaggcaaaaa tggcgcagtg
    11581 aaagataaaa aaacaggcgg caaagaggcg gcaaaagagg gcaaagagtc caaaaaaacc
    11641 gccaaaaacc gcaaagaagc agagaaggag gcgacatcca ggcagtctgc gcgcaaagga
    11701 cgcgaagggg ataagaaatc gaaggcggaa cacaaaaagg cacatggcaa gcccgtgtcc
    11761 ggatccaaag aaaaaaacgc aaaaacacag cctgaaaaca agcaaggcaa aaaagaggca
    11821 aaaggacagg gcaatccgcg caagggcggc aaggcggaaa aagacactgt ttctgcaaat
    11881 aaaaaagtcc gttccgacaa gaacggcaaa gcagtgaaac aggacaaaaa atacagggaa
    11941 gagaaaaatg ccaaaaccga ttccgacgaa ttgaaagccg ccgttgccgc tgccaccaat
    12001 gatgtcgaaa acaaaaaagc cctgctcaaa caaagcgaag gaatgctgct tcatgtcagc
    12061 aattccctca aacagcttca ggaagagcgt atccgccaag aacgtatccg ccaagagcgt
    12121 atccgtcagg cgcgcggcaa ccttgcttcc gtcaaccgca aacagcgcga ggcttgggac
    12181 aagttccaaa aactcaatgc cgagctgaac cgtttgaaaa cggaagtcgc cgctacgaaa
    12241 gcgcagattt cccgtttcgt atcggggaac tataaaaaca gccagccgaa tgcggttgcc
    12301 ctgttcctga aaaacgccga accgggtcag aaaaaccgct ttttgcgtta tacgcgttat
    12361 gtaaacgcct ccaatcggga agttgtcaag gatttggaaa aacagcagaa ggctttggcg
    12421 gtacaagagc agaaaatcaa caatgagctt gcccgtttga agaaaattca ggcaaacgtg
    12481 caatctctgc tgaaaaaaca gggtgtaacc gatgcggcgg aacagacgga aagccgcaga
    12541 cagaatgcca aaatcgccaa agatgcccga aaactgctgg aacagaaagg gaacgagcag
    12601 cagctgaaca agctcctgag caatttggag aagaaaaagg ccgaacaccg cattcaggat
    12661 gcggaagcaa aaagaaaatt ggctgaagcc agactggcgg cagccgaaaa agccagaaaa
    12721 gaagcggcgc agcagaaggc tgaagcacga cgtgcggaaa tgtccaacct gaccgccgaa
    12781 gacaggaaca tccaagcgcc ttcggttatg ggtatcggca gtgccgacgg tttcagccgc
    12841 atgcaaggac gtttgaaaaa accggttgac ggtgtgccga ccggactttt cgggcagaac
    12901 cggagcggcg gcgatgtttg gaaaggcgtg ttctattcca ctgcaccggc aacggttgaa
    12961 agcattgcgc cgggaacggt aagctatgcg gacgagttgg acggctacgg caaagtggtc
    13021 gtggtcgatc acggcgagaa ctacatcagc atctatgccg gtttgagcga aatttccgtc
    13081 ggcaagggtt atatggtcgc ggcaggaagc aaaatcggct cgagcgggtc gctgccggac
    13141 ggggaagagg ggctttacct gcaaatacgt tatcaaggtc aggtattgaa cccttcgagc
    13201 tggatacgtt gatcggattg ccgaaacggt aggctttatg caaatcgtgc cgatatggtg
    13261 cgctgttccg tgaagtttgc cgtacctgta ttttccggtt tttggaaatc aagagagaaa
    13321 gttaaagaag aatgtcgaaa cctgttttta agaaaatcgc actttatact ttgggtgcaa
    13381 tcagcggcgt ggccgtcagt ctggcggtgc agggttttgc cgccgagaag gacaggcggg
    13441 ataacgaagt cctgccggtg caatccatcc gcacaatggc ggaggtttac ggtcaaatca
    13501 aggcaaacta ctatcaggac aaacccgatg ccgatttgtt tgaaggtgcg atgaagggta
    13561 tggtggccgg tttggatccg cattccgaat atatggataa aaaaggttat gccgagataa
    13621 aggagtccac cagcggcgaa tttggcggct tggggatgga aatcgggcag gaagacggat
    13681 ttgtcaaagt ggtttcgccg attgaggaca cgcctgcgga acaggcgggg gtgaaaagcg
    13741 gcgatttcat tgtgaaaatc gataatgttt cgacacgcgg catgacggtc agcgaagcgg
    13801 tgaagaaaat gcggggcaag ccgggtacga agattacttt gacgctgtcg cgcaaaaatg
    13861 ccgacaagcc gatagtcgtc aacctgaccc gtgccattat taaagtgaaa agcgtccgcc
    13921 atcacctgat cgaacccgat tacggctata tccgcgtgtc gcagttccaa gagcggacgg
    13981 tcgaaagcgt caataccgcc gcaaaagagc tggtaaagga aaataaagga aaaccgctca
    14041 aggggctggt gttggatttg cgcgacgacc ccggcgggct tttgactggc gcggtcggcg
    14101 tgtcggcggc atttctgccg tctgaagcag tcgtcgtcag tacgaaaggg cgggaaggta
    14161 tggaaggctt tgtgttaaag gcgcgtcccg aagattatat ttttaaagcc ggcgcggatc
    14221 ctttggcggg tattcctgcc gagttgaaaa ccataccgat gacggttctg attaattcgg
    14281 gttcggcttc cgcgtcggag attgtcgcag gtgcattgca ggatcataaa cgcgcggtta
    14341 tcgtcggtac gcagagcttc ggcaaaggtt cggttcagac tttgattcct ttgtccaacg
    14401 gcagcgcggt caagctgaca acggcactgt attatacgcc gaacgaccgt tctattcagg
    14461 cgcaggggat tgttcccgat gtcgaagtaa aagataagga acgcattttt gaaagccgcg
    14521 aggcggattt ggtcggacac atcggcaatc ccttgggcgg cgaggatgtg aacggtgaaa
    14581 cccttgtcgt gccgcttgaa aaagatgcgg ataagcccgc tgtaaaagaa aaaggtaaaa
    14641 agaaaaagga cgaggatttg tcttcaaggc ggattcccaa ccctgccaaa gacgaccagt
    14701 tgcggaaagc tttggattta gtcaagtcgc cagaacagtg gcagaagtct ttggggctgg
    14761 cggcgaaaaa gccggtttca aataaagata agaaagataa gaagtaggtt ttgacgatgc
    14821 cgtctgaaag gatttcagac ggcatattat gtttgaaagg acaggttatg actgattttt
    14881 taccggaatt tgaaccgtgc ggcatttata gtggattaac aaaaatcagg acaaggcaac
    14941 gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact ggtttttgtt
    15001 aatccactat acttgggtag cgattgggtt tcagacggca tacattcagg atttcaccgc
    15061 cttatgccgt ctgaaagacg tttccgaccc atccctacta cacgctttgt ccgcaaacgc
    15121 atcacaggca agattatgga agtcatccaa taccccgatt cccccttcaa actccaccaa
    15181 cccttcccgc ccgccggcga ccagcccacc gccattgccg gcctgctcga agggctttca
    15241 gacggccttg cctatcaaac cctgctcggc gtaaccggtt cgggcaaaac ctacaccatg
    15301 gcgaacgtca tcgcccaaag cggccgaccc gccatcatca tggcgcacaa caaaaccctt
    15361 gccgcccagc tctatgccga aatgcgcgag tttttccccg aaaacgcggt ggaatatttc
    15421 gtctcctact acgactatta ccaacccgaa gcctatgtgc ccagccgcga cttgttcatc
    15481 gaaaaagaca gcgcgatcaa cgaacacatc gagcagatgc gcctttccgc caccaaaaac
    15541 ctgatgacgc gcgacgacgt gattatcgtc gccaccgtgt ctgccattta cggcatcggc
    15601 gacccgaccg aatatcaaca aatggtgttg tccgtcaaag aaggcgacac aatcgaacaa
    15661 cgcgacatca tcgccacgct cgtttccatg cagtacgaac gcggcgattt ggacttcaaa
    15721 cgcggcagct tccgcgtgcg cggcgacgtg attgacgtgt accccgccga aagctccgaa
    15781 aacgccctgc gcatcagcct gttcgacgac gaaattgacc gcctcgatat gttcgacccg
    15841 ctttcaggca gccttatcca acgcgtcggc cgctacaccg tcttcccgtc cagccactac
    15901 gttaccccgc gcgacaccgt attgcgcgcc tgcgagtcca tcaaagaaga attgcgcgaa
    15961 cgcattgatt tctttaccaa ggaaaaccgc cccgtcgaac aacaacgcat cgaacagcgc
    16021 acccgcttcg accttgaaat gctctacgaa atgggctttt gcaaaggcat cgaaaactac
    16081 tcccgccact tctccggcaa aaaagaaggc gaaccgccgc ccacgctgat ggactatctg
    16141 cccgacaacg ccatcatgtt cattgacgaa agccacgtta ccgttaccca aatcggcggc
    16201 atgtacaaag gcgacgcatc gcgcaagcaa aacctcgtcg actacggctt ccgcctgcct
    16261 tccgcccgtg acaaccgccc gctcaaattc cacgaatttg aaaaagtcat gccgcaaacc
    16321 gtcttcgtgt ctgccacccc cgccaaatac gaagaagaac acgccggaca agtggtcgaa
    16381 caagtcgtcc gccccacagg gctggtcgac ccccaaatca tcatccgccc cgtcgccacc
    16441 caagtcgatg acttgatgag cgaaatcaac gaccgcatcc aaaaaggcga acgcgtgctc
    16501 gttaccaccc tcaccaaacg catggcggaa caactcaccg actattacag cgaactcggc
    16561 atcaaagtgc gctacctgca cagcgatatc gacacggtag agcgcgttga aatcattaga
    16621 gatttacgga tcggattgtt cgatgtactc gtcggtatca acctcttgcg cgaaggcctc
    16681 gacatccccg aagtctccct cgtcgccatt cttgacgccg acaaagaagg cttcctacgc
    16741 tcccaccgca gcctgattca aaccataggc cgcgccgccc gcaacgtgaa cggcgtcgcc
    16801 atcttgtacg ccgacaaaat caccgactcc atgaaagccg ctatagacga aactgaacgc
    16861 cgccgcgaaa aacagatgaa attcaacgaa gaacacggca tcgtaccgca gcagattaaa
    16921 aaacaggtca aagacatcat cgacggcgtg taccacgaag aagacagcgg caaaggtcgt
    16981 ctgaaaggta aaaacaaggt taaagtcggc gaaatccaca acgaagaaga cgcgattaaa
    17041 gaaatcgcca aactggaaaa agccatgcag caggcggcta gggatttgca gtttgaagaa
    17101 gcggcggtgt tgagggatag gattaggaat attaaagaga atttgttgtt tggggcagag
    17161 taaaggagct tgatttttta attagcgaag atacaataac gtcatctatt atttttatag
    17221 gaaagtaact atggcagagc aactacagct gattcctcgt gttgaaagcg gtgaaattat
    17281 cccacagaga atgagtgacg gatatatcaa tgcgacagca ttgtgtaaat ctgtaggtaa
    17341 atcttacagt gattatagac aattacagtc tacaaatcat ttcttaaatg aattgaaagc
    17401 ccagacggga ttatccgaac aacaattgat tcaacagaga attggtggag aaccttcttt
    17461 acagggctct tgggtacacc cttatttagc aattaattta gcacagtggt tatcccctgc
    17521 atttgctgta aaggtatcta cttgggttca tgaatggatg tcaggtaaag tcaatgcttc
    17581 tccttcccat tccgcgcata tccaaagata cttgcaaaat agaggaaaaa ttccatatac
    17641 gcatttttca atgcttaatg aaattacgct aaatttaatt gctcctttgg aggaacaagg
    17701 ttatgtatta cctagtcagt tagttccgga tatttcagaa ggaagattat tttgtagatg
    17761 gctgagggaa aatagaaacg ttgatccgaa taacttccca acatataaac atgaattcct
    17821 tgatgctcgg agacccgtgg aggccaaatt atatccaata gaatactatc aagattttaa
    17881 atggcacttt aatgaagtat ggttaaaaga aaaagctaaa caatattttt cagaacgtga
    17941 tgcagcaatt ttgccttatt taaccccgtt aatgttacca gcaagcgagt cgtagcaagc
    18001 cgtagcctgc attaccaacc accgcgtgcg tagctacgcc atacacccta cctgcgggcg
    18061 ggacaaactt taaggggctg tactagatta gccctaaatc ccataccaat cccgcaggat
    18121 tttaagctgt tgagacggtg tgccgaagtt aaatcgaaat tagcattctt tcaagaacag
    18181 tggaaaagat ttgcaatcga ttccgttgta ttttcgcaag acgcgttttg cc
//
LOCUS       DAXHKX010000023        17679 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHKX010000023 DAXHKX010000000
VERSION     DAXHKX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.023"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(108..809)
                     /locus_tag="ACFMWZ_000657"
     CDS             complement(108..809)
                     /locus_tag="ACFMWZ_000657"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC5934652.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFECAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            977..2107
                     /locus_tag="ACFMWZ_000658"
     CDS             977..2107
                     /locus_tag="ACFMWZ_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC5934653.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            2141..3118
                     /locus_tag="ACFMWZ_000659"
     CDS             2141..3118
                     /locus_tag="ACFMWZ_000659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC5934654.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDESENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVVLIHPAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            3122..3472
                     /locus_tag="ACFMWZ_000660"
     CDS             3122..3472
                     /locus_tag="ACFMWZ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC5934655.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            3477..4256
                     /locus_tag="ACFMWZ_000661"
     CDS             3477..4256
                     /locus_tag="ACFMWZ_000661"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC5934656.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFQKVPDMRTV"
     gene            4282..4497
                     /locus_tag="ACFMWZ_000662"
     CDS             4282..4497
                     /locus_tag="ACFMWZ_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC5934657.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            4598..4909
                     /gene="grxD"
                     /locus_tag="ACFMWZ_000663"
     CDS             4598..4909
                     /gene="grxD"
                     /locus_tag="ACFMWZ_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC5934658.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            5015..5641
                     /gene="upp"
                     /locus_tag="ACFMWZ_000664"
     CDS             5015..5641
                     /gene="upp"
                     /locus_tag="ACFMWZ_000664"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC5934659.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            5664..5984
                     /locus_tag="ACFMWZ_000665"
     CDS             5664..5984
                     /locus_tag="ACFMWZ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC5934660.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            6235..6654
                     /locus_tag="ACFMWZ_000666"
     CDS             6235..6654
                     /locus_tag="ACFMWZ_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC5934661.1"
                     /translation="MKKMFLSAVLLLSAATRTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            6675..7436
                     /locus_tag="ACFMWZ_000667"
     CDS             6675..7436
                     /locus_tag="ACFMWZ_000667"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC5934662.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            7511..8785
                     /locus_tag="ACFMWZ_000668"
     CDS             7511..8785
                     /locus_tag="ACFMWZ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC5934663.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALG
                     LGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLD
                     GNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLMLDG
                     VLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            8782..9999
                     /locus_tag="ACFMWZ_000669"
     CDS             8782..9999
                     /locus_tag="ACFMWZ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC5934664.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSSETC"
     gene            10074..11138
                     /gene="hemE"
                     /locus_tag="ACFMWZ_000670"
     CDS             10074..11138
                     /gene="hemE"
                     /locus_tag="ACFMWZ_000670"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC5934665.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            11324..12703
                     /gene="radA"
                     /locus_tag="ACFMWZ_000671"
     CDS             11324..12703
                     /gene="radA"
                     /locus_tag="ACFMWZ_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC5934666.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMSELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(12979..13458)
                     /locus_tag="ACFMWZ_000672"
     CDS             complement(12979..13458)
                     /locus_tag="ACFMWZ_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC5934667.1"
                     /translation="MKHILPLIAASALCISTASAHPAGKPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(13590..13949)
                     /locus_tag="ACFMWZ_000673"
     CDS             complement(13590..13949)
                     /locus_tag="ACFMWZ_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC5934668.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIIRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(13988..17602)
                     /gene="recB"
                     /locus_tag="ACFMWZ_000674"
     CDS             complement(13988..17602)
                     /gene="recB"
                     /locus_tag="ACFMWZ_000674"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC5934669.1"
                     /translation="MSAPIQAFDSLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLILAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLASDKLEARLKKGKTPDAAVFAELQKLADFGYD
                     LNALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAGDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGLPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTEDAPPPAAYRGNAGQTAE
                     FTANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAEISVPAMPSETPTAS
                     DGISIHNFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSNIPPEYRCPEMGFTLFTENFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         4019 a         4615 c         5056 g         3989 t
ORIGIN      
        1 aaatgccgtc tgaacaaaca gcggtgcgtc cgaatatcgg gcgcaacgtg gaaatagccc
       61 gatattctaa cggaaaaccg ctgttttggc atgatttgat aaaggtttta taaagatttg
      121 acgatttctg ccaccatttt tgcagaactt gccgccgccg ttttcaaaaa ttcgtcaaag
      181 ctgatgtctg ccttttcgtc cgccgaatcg gaaaccgcgc ggatgatgac gaaaggcgtt
      241 tccaactgat gacaggtttg ggcaatcgcc gccgcttcca tttccactgc tttgactttg
      301 gggaagtgct tgcggatttc cgccacgcct tcgctgctgt ggacgaagcg gtcgccgctg
      361 acaatcaggc cttgttctac cgccgcgcat tcaaacactt ccgccgcctg ttttgctttg
      421 ccgaccaaga ggccgtctga aacaaatacg gcgggcagtt gcggcacttg tccccaaaca
      481 tagccgaatg cggttacatc gacatcgtgg tgcgcgattt ctgtgccgac cacgacatcg
      541 ccgactttca aacccttgcc caagccgccc gcgctgccgg tgttgataac gcagtccggt
      601 gcgaattcac ggataatcca agccgttgca accgccgcgt tgaccttgcc gatgccgctc
      661 aatgcaagca ccatgcgttt tcccgctaat tcgccttcat aggcggcaaa cttgccgaaa
      721 gagacggctt tgacattttc catcatctcg cgcaaaagct cgatttcttg ttccattgcg
      781 ccgataacgg ctactgtttt caaagacata ttgctgacct gttgtgaatt tcggatagaa
      841 tgcctgatta tacacgctaa cacggcagga ttgagtgggg gtggtttgtc catgccgtct
      901 gaaacggttt cagacggcat ggcgggtttt tggtagaatg ggaaggtaca gattgtttga
      961 agattgaggg acgaggatgt ttaccgatga aaatatgacc gcaaaggaag aactgttcgc
     1021 atggctgcgc catatgaaca aaaacaaagg ttccgacctg tttgtgacga cccatttccc
     1081 gcccgcaatg aagctggacg gcaaaatcac ccgcatcacg gacgagccgc tgacggcgga
     1141 aaaatgtatg gaaatcgcct tttcgattat gagtgcgaag caggcggaag aattttcatc
     1201 gaccaacgag tgcaacttcg ccatcagcct gccggacacc agccgcttcc gcgtcaatgc
     1261 gatgatacag cgcggcgcga cggcgttggt attccgcgcg attaccagca agattcccaa
     1321 gtttgaaagc ctgaacctgc cgccggtctt gaaggatgtc gcgctgaaaa aacgcgggct
     1381 ggttattttt gtcggcggta ccggttcggg caaatcgact tcgcttgcct cgcttatcga
     1441 ttaccgcaat gaaaattcgt tcggacacat catcaccatc gaagacccga tcgagtttgt
     1501 ccacgaacac aaaaactgca tcatcaccca gcgcgaggtc ggcgtggata cggaaaactg
     1561 gatggcggcg ttgaaaaaca cgctgcgtca ggcgccggat gtcatcctta tcggcgaaat
     1621 ccgcgaccgc gaaacaatgg actacgccat tgcctttgcc gaaacggggc atttgtgtat
     1681 ggcgacgctg cacgccaaca gcaccaatca ggcactcgac cgcatcatca actttttccc
     1741 cgaggagcgg cgcgaacaat tgctgacgga tttgtcgctc aacctgcagg cgtttatttc
     1801 gcaacgcctc gttccgcgag acggcggcaa gggcagggtg gcggcagtcg aggtgctgct
     1861 caattcgccc ctgatttcgg agttgattca caacggcaac atccatgaaa tcaaagaagt
     1921 gatgaaaaaa tccactaccc tgggtatgca gaccttcgat caacaccttt accaattgta
     1981 tgaaaaaggc gatatttccc tgcaagaagc attgaaaaat gccgattccg cacacgattt
     2041 gcgtttggcg gtacagttgc gcagccgccg cgcgcaaagt tccagccccg atttggaact
     2101 gctctgatgg cggtatggat ttccggacgg atggtttgaa atgatttatc cgtggcataa
     2161 tgagcaatgg cggcagattg cggaacattg ggagcgtcgt cccaatgcat ggctgtttgc
     2221 cggcaaaaaa gatacgggga aaactacatt tgcccgcttt gcggcgaagg cactgttgtg
     2281 cgaaacccct gcaccgggct gcaaaccctg tggcgaatgt atgtcctgcc atctgtttgg
     2341 acagggaagc catccggatt tttacgaaat cactcccttg tcggacgaat ccgaaaacgg
     2401 gcgcaaactg ttgcagatca aaatcgatgc cgtcagggaa atcatcgata atgtgtacct
     2461 gacttcggta cggggcggtt tgcgcgtggt tctgattcat cctgcggaaa gtatgaatat
     2521 ccaggccgcc aacagtttgt tgaaagtgtt ggaagagcct ccgccacaag tggtcttttt
     2581 gctggtcagc cacgcggcgg acaaggtttt accgaccatt aaaagccgct gccggaaaat
     2641 ggttttgccc gcgccttccc atgaagaggc attggcatat ctgcgtgaaa ggggtgtggc
     2701 ggaacctgag gaacgtctgg ctttccattc cggagcgccg ctgtttgatg aggcggacgg
     2761 tgtccgtgcg ttgcggatta aactgttgga tattttggca gaaccgaggt tgttgaagat
     2821 tttggattac gccgcgcttt tcgataagga aaaacttccg ctcgccgtat ttgtcgggtg
     2881 gatgcagaaa tggctggtcg atttgggatt gtgcctgcaa cacatgaaac ccgtctatta
     2941 tcccgcttat gaagacaggc tgcttcagac ggcatccggt ttccgcccgc gcaatgtatt
     3001 tgcggcggag gatatgctca aacagcttgc cccctacggg tttcatactt taaatgtcaa
     3061 aatgcagatc gagcatctgc tcatcaatta tttggaattg aagaaagaga acgggtgaat
     3121 tatgtcagac ggacaaaata ttccggcaaa aatgatgtcg ttgcagctga aagacatgaa
     3181 tctgctgtac agctcctata tgccgttttt ggaacatggc ggcctgtttg tgcagaccga
     3241 cgacgtattt tccatcgggg acgatattct gcttgccgta gaaatcctca acttccccaa
     3301 actgttcctg ccaaccaaag tcgcctggat caatcctgcg cgtacttcct ccaaacccaa
     3361 aggggtgggg ctggcattca caaaacacga aaactgcctg aaagtcaaag accagatcga
     3421 agtcgaactg ggcaacacaa tcggcggcag cagacctacg tttaccatgt aacgccatgc
     3481 atatcatcga ttcgcactgc cacctcaatt ttgaaggttt gaaagaacgc ctgcccgaag
     3541 ttttgtccaa catggaagca aacggcgtgg ggcaggcgct cgccatcagc gtcagcaggg
     3601 aaagttttgc cgaagtatcc gctatcgccg aagtttatga acatatttac tgcacaatag
     3661 gcgtacatcc cgacagcaag gaagccgaag aattttctat tgcggaaatg gttgctgcag
     3721 ccgcccatcc gaaagtggtc ggcatcggcg agacgggctt ggattattac tggtgcaaag
     3781 gcgatttgtc ctggcagcac aaacgctttg cagaccacat cgaagcagcc aatcaaaccg
     3841 gactgcccgt tatcgtccat acgcgtgatg cggcggcgga caccttgtct atcctgaaag
     3901 aatgccgggt taattcgggc gttatccact gtttttccga agacatcggt tttgcccgtg
     3961 cagcaatgga tttggggctt tacatttctt tctcgggaat cgttaccttt aaaaacgcac
     4021 ccttggttca ggaggcggcg aaatacgttc cggacgaccg cattttagtg gaaaccgatg
     4081 cgccgttcct cgcccccgta cccaagcgcg gcaggcagaa cgaaccggct ttcgtgcgtt
     4141 atactgccga acatatcgcc aaattgcgga accaaacctt ggaacaggtt gcggcataca
     4201 cgacggaaaa cttttaccgg ctgtttcaaa aagtacccga tatgcggacc gtctgaccct
     4261 gtaccgacga taaggaaaac catgaaggca attcatccgt atgcatgtcc gcgctgctgc
     4321 cggctgcctg ccaacacgtt tcggacaggc atggcaaatt ccgcttccaa attctgcatt
     4381 gccaaaggcg gcagacggga agtaaaaaaa gacgaaagcg gcggcggata tgccctgtgc
     4441 catttgccgg acagcaggat tgtcgaggag tgggaatatt tccgttcaca acattgatac
     4501 tgcgcgatat acggcaaata ttgtgggaag tttccgcttt tgcgtataat gcgtcctacc
     4561 tgacaaattt tgtcaacttt atcaaaagga ataagcgatg gcttccatcc acgaccaaat
     4621 taaagaagta gtaacgacac accgcgtcgt attgtttatg aaaggtacga agcagtttcc
     4681 gcaatgcggt ttctcttccc gcgccgtgca aatcctgaac gcggcaggct gcaccgatta
     4741 cgttaccgtc aacgtattgg aaaatcccga agtacgccaa ggcattaagg aatacagcga
     4801 ctggccgacc atcccccaac tttatgtgaa cggcgagttt gtcggcggtt cggacatcct
     4861 gatggaaatg tatgaggcag gcgagctgca agagctgctg aaagcctgat ggattcggca
     4921 atgccgtctg aacgtgtttc agacggcatt ttcttttccg gcaaatcaaa aaaaagtata
     4981 atggcgcgtc tcaaaatcac actggaacac cgcgatgaac gttaatgtta tcaaccatcc
     5041 gctcgtccgc cacaaattaa ccctgatgag ggaggcggat tgcagcacct acaaattccg
     5101 gacgcttgcc atcgagctgg cgcgcctgat ggcatacgag gcaagccgtg attttgaaat
     5161 cgaaaaatac cttatcgacg gatggtgcgg tcagattgaa ggcgatcgca tcaagggcaa
     5221 aacattgacc gtcgttccca tcctgcgtgc aggtttgggt atgcttgacg gcgtactcga
     5281 cctgattccg actgccaaaa tcagcgtagt cggactgcag cgcgacgagg aaacgctgaa
     5341 acccgtttcc tacttcgaga aattcgtgga cagcatggac gaacgtcctg ccctgattat
     5401 cgacccgatg ctggcgacgg gcggttcgat ggttgccacc atcgacctgt tgaaagccaa
     5461 gggctgcaaa aacatcaagg cactggtgct ggttgccgcg cccgagggtg tgaaggcggt
     5521 caacgacgcg catcccgacg ttacgattta caccgccgcg ctcgacagcc acttgaacga
     5581 gaacggctac atcatccccg gcttgggcga tgcgggcgac aagattttcg gcacgcgcta
     5641 actgactgat tttcggattt gatatgaatt ttcaagacta tctcgccaca ttttcttcaa
     5701 tcgaccatct gggcggtttg gacgttcagg atgcagatgg caaaacggtt caccacattc
     5761 ctgccgttca gggtaagctc ggttcgctca agttgtacaa cgctttggca gaacgttttg
     5821 acggaaaatt ggacaaagaa gcggcagaac agggtttgat atggtttgcc gaacacgttg
     5881 ccgacgcgcg cgcccatccc ggcaagcatc ccaatatcga cctgcttgaa aaagttgtgc
     5941 aaagcggcga aaccttgctg ctcaagccgc ttgccgcgca ataattttcg accatgccgt
     6001 ctgaaatccg tttcagacgg cattttgtcg gaaagaagac cgtaaaacgg gcattttctt
     6061 ttctatttca ggatacgggc aatgatgttt caacacacag gacgacacat aaagcgttgc
     6121 cctatgtgtt gccctaattt ggaaggggtt acacccctct caaataacat ctgatgctgc
     6181 tgccacgaag gacggatgtc caaatggcgg agtttcaacc attaaggaaa tacgatgaaa
     6241 aaaatgttcc tttctgccgt attgcttctg tcggctgcga cccgaaccgt gtgggcggat
     6301 acggtgtttt cctgtaaaac ggacaacaac aaatatatag aagttcaaaa aatcaaccgc
     6361 aatctttacg aatattcgtt cggtagtgcg gcaaaaaaag aaattgccat acgcaacagc
     6421 aaagctgacc tgttggggcg ttccgacagg tggcaaggta tgggcagcgg tcgttgggca
     6481 acgatgaaat tccaaaacgg cgaatttatg tacaccatat ggacaggctt cgattccgtg
     6541 actcatacgg aaagcagcgg tgtcgttgtg gagcgtaggg gtaaggaagt cgcacgggtc
     6601 ggctgtacgc cgaaaaccgc gcaggcgaat ttcaacgatg acgatttttc ctagtaatcg
     6661 gggcggataa ggcgatggaa acagcgaaac ccgtcatgct gattgtccgt ccgtcgggca
     6721 gggccgcaga agatgtcgaa acttgcctga atgccggttg gcgcgcggaa gtattgagtc
     6781 cggtcgaaat cgaagcagat gctgccggac tggaactttt gtccgaacaa tatgcccgtg
     6841 cggatgccgt gttttgggtc agtccgaccg ccgttgaaac cgccgtcccg taccttaacc
     6901 tttcagacgg cataaaggcg cagattgccg tagggcaggg cagccgccgc gcattggaac
     6961 gctgtttggt cagaacggtc atcgcgcctg atgacggcaa cgacagcgag gcggttttgc
     7021 gcctgccggt ttggaacagt ctgcccgaag gtgcgcgcgt attgtttgtg cgcggacacg
     7081 gcgggcggga ttttttgatg aatgccttgc aggagaaagg ttttcggacg gaggtggcag
     7141 aagtctattt cagacggcat aaacctttga actttcaaaa tttccaaacc gaaaatattg
     7201 ccgccgccta tattacgtcg accgagctgg tgcgcttgct gttcgggcag cttccgccgc
     7261 aattttcccg attcttcaaa tccttgctat actttaccca tcatccgcgc attgcggagg
     7321 cattgaagcg cgaaggcgtg tgttcggtcg aaaccgtccc tacgctggaa gccgcgcttt
     7381 cccattcttc catttccgtt tcagacggca tggtctttcc cggaacctca aattaataag
     7441 gagcaaaacg gtgggcgaac ctgaaaacaa atcatccgaa cccgtacgcg agatacaggc
     7501 atcaaaagaa atgccgtctg aaacctcttc cccacgcaaa gaaaacgaaa cagaagtaca
     7561 cattcctgcc gctcctttta tcgtcaaaca gtccggcagc aacgctttgg cagtctgcgc
     7621 cctggtattg gcggcattgg gtttgggtac aagtggtttt ttgtttgtcc aaggacagaa
     7681 tgtcttgaaa aaccaagagc tggcattcaa ccaaaaaatc gacaaagccg ccttgggcga
     7741 gtcggaaaac gccgccctgt tgaaagacaa cctcaaccgg caagccgcca tacaatcaga
     7801 gctcgaccgt ttggacggaa acgtcaaagc aaacggcgaa caaatcttgg aaatgcaaaa
     7861 atcctatcgc gagttgacca aaggacgcgc cgattggctg gtggacgaaa ccgagaccat
     7921 actcaatctg gcggcgcaac agctggtgtt gactggcaat atccaaacgg cagtcggcgt
     7981 attggagcat atcgacagcc gcctgtcccg tttcaatcag gcagagcttc tgccgatcaa
     8041 gcaggcggtc agcagcgact tggcggaact gaaaaaccgt ccctatgtcg atatttccgg
     8101 cacggcattg cgcctcgaca ggctggaaac cgccgtatcc ggactgccgc tgatgctcga
     8161 cggcgtattg aaaccgggcg tacaggtgaa gaacgaagcc gcttctgctt catggtggca
     8221 gaacgtatgg gaaaaatccc tcggcacatt gaaggggctg gtcgaaatcc gccgtttgga
     8281 aaacaacgat gccatgctga tttctcccga acaggcatat tttgtgcgtg aaaacctgcg
     8341 cctccgcctt ttggatgcgc ggactgcatt aatgcagcgc aacagcgaag tctatcaggg
     8401 cgatttgaac aatgccgaag ccgccgtcag acagtatttc gatgccaagt ctcccgccac
     8461 gcagtcgtgg ctgaaagaac tggcggaatt gaaggcgttg gatgtgcgca tgacggcgga
     8521 tgacggtttg aaaaacagcc taaatgccgt ccgcgcctat cgcgacggta cgcgcatgac
     8581 ggcggcggaa aatcaagaag cagaacaggc ggcttccgaa ccggcaaacg aaaacacagc
     8641 ttccgaaccg gctgccgcat cggatgtgaa gaccatagaa gcaccgtccc tgccttcgga
     8701 acgcaaaccg gaacagcctg caaaaaaaca gcccgtaccg gaaaaggcag ggcgttcgcc
     8761 gtccgctaaa ggagaacgcg catgaaaacg gtagtctgga ttgtcgtcct gtttgccgcc
     8821 gccgtcgggc tggcattggc ttcgggcatt tacaccggcg acgtgtatat cgtactcgga
     8881 cagaccatgc tcagaatcaa cctgcacgcc tttgtgttag gttcgctgat tgccgtcgtg
     8941 gtgtggtatt tcctgttcaa attcatcatc ggcgtactca atatccccga aaagatgcag
     9001 cgtttcggtt cggcgcgtaa aggccgcaag gccgcgcttg ctttgaacaa ggcgggtttg
     9061 gcgtattttg aagggcgttt tgaaaaggcg gaacttgaag cctcgcgcgt attgggaaac
     9121 aaagaggcgg gggataaccg gactttggca ttgatgttgg gcgcacatgc cgccgggcag
     9181 atggaaaaca tcgagctgcg cgaccgttat cttgcggaaa tcgccaaact gccggaaaag
     9241 cagcagcttt cccgttatct tttgttggcg gaatcggcgt tgaaccggcg cgattacgaa
     9301 gcggcggaag ccaatcttca tgcggcggcg aagatgaatg ccaaccttac gcgcctcgtg
     9361 cgtctgcaac ttcgttacgc tttcgacagg ggcgacgcgt tgcaggttct ggcaaaaacc
     9421 gaaaaacttt ccaaggcggg cgcgttgggc aaatcggaaa tggaacggta tcaaaattgg
     9481 gcataccgcc gccagctggc ggatgctgcc gatgccgccg ctttgaaaac ctgcctgaag
     9541 cggattcccg acagcctcaa aaacggggaa ttgagcgtat cggttgcgga aaagtacgaa
     9601 cgtttgggac tgtatgccga tgcggtcaaa tgggtcaaac agcattatcc gcacaaccgc
     9661 cgccccgagc ttttggaagc ctttgtcgaa agcgtgcgct ttttgggcga gcgcgaacag
     9721 cagaaagcca tcgattttgc cgatgcttgg ctgaaagaac agcccgataa cgcgcttctg
     9781 ctgatgtatc tcggtcggct cgcctacggc cgcaaacttt ggggcaaggc aaaaggctac
     9841 cttgaagcga gcattgcatt aaagccgagt atttccgcgc gtttggttct ggcaaaggtt
     9901 tttgacgaaa ccggagaacc gcagaaggcg gaggcgcagc gcaacttggt tttggcaagc
     9961 gttgccgagg aaaaccgccc ttcctccgaa acctgttgaa accttcagac ggcatcgttg
    10021 agatgccgtc tgaaaaccat ttcacttgac aaacagactg acagaaaacc cgtatgactc
    10081 ttttaaaaaa cgatactttt ctccgcgccc tgctcaaaca gcccgtcgaa tacacgccga
    10141 tttggatgat gcgccaggcg gggcgttatc tgcccgaata caaagccaca cgcgcgaaag
    10201 cgggcagctt cctcgatttg tgcaaaaaca ccgaattggc gaccgaagtt accatccaac
    10261 ctttggaacg cttcgatttg gacgcggcga ttttgttttc cgacatcctg accgtccccg
    10321 acgcaatggg cttgggactg tattttgccg aaggcgaagg cccgaaattc aaacgcgccc
    10381 tgcaacacga ggacgacatt gccaaattgc acgtccccga tatggaaaaa ctgcaatacg
    10441 tctttgatgc cgtcagctcc atccgtaaag cactggacgg ccgcgtaccg cttatcggct
    10501 tctccggcag cccgttcacg ctcgcctgtt atatggtcga aggcggcggc agcaaagaat
    10561 tccgcaccat caaaaccatg atgtactcgc gccccgattt gttgcacaaa atcctcgata
    10621 ccaacgccca agccgttacc gcctacctca acgcccaaat cgacgcgggc gcgcaggcgg
    10681 tgcagatttt cgacacttgg ggcggcgtgt tgagcgatgc ggcgtttaaa gaattcagcc
    10741 tcaaatacat ccgccagatc gtcgccggac tcaaacgcga aagcgaaggc cgccgcgtgc
    10801 ctgttatcgt atttgccaaa ggcggcgggc tgtggctgga aagtatggcc caaatcggcg
    10861 cagacgcatt gggcttggac tggacgtgca acatcggcga agcacgccgc cgcgtcggca
    10921 agcaagtcgc cctgcaaggc aacttcgacc cgttcgccct cttcggtacg ccggaatcca
    10981 tccgcaccga ggtcgcacgt atcctagccg actacggaca cggcagcggc catgtcttca
    11041 acctcggaca cggcatcaac caacacgccg accccgaaca cgccaaaatc ttagtcgata
    11101 ccgtacacga gctgtcgcgg cagtatcacg gcgggtaagc cggcaggaaa ccgcccgata
    11161 tgccgtctga agccgagaga tggccggtta gggtaaaaat aaggcaatgc ggcaatatcc
    11221 gccgtgtacg gatagtacat gacggcggcg ttgtcgtatt ggcgcaatcc caaccgtccc
    11281 tatgttcaga cggcattttt gttttcagat gcagggaaaa ccgatggcaa aaacgcttaa
    11341 aaccctttac caatgcaccg aatgcggcgg cacttcgccg aaatggcaag gcaaatgccc
    11401 gcattgcggc gagtggaaca cgcttcaaga aagcttagcc gcgcccgagc cgaaaaacgc
    11461 ccgcttccaa tcttgggcgg cggatacctc gaccgtccaa tccctctccg ccgttaccgc
    11521 tgccgaagtg ccgcgcaacc cgaccggcat gagcgaactc gaccgcgtat tgggcggcgg
    11581 tttggtcgat ggcgcggtca tcctgctcgg tggcgatccc ggcatcggca aatccacgct
    11641 gttgttgcaa accatcgcca aaatggcgca aagccgcaaa gtgctgtacg tttccggcga
    11701 ggaatccgcc caacaagtcg ccctgcgcgc gcagcgtttg gaactgccca ccgaaggcgt
    11761 gaacctgctt gccgaaatcc gcatggaagc cattcagacg gccttgaaac agcatcagcc
    11821 cgaagtcgtg gttatcgatt ccatccaaac catgtattcc gaccaaatca cttccgcccc
    11881 cggctccgtg tcgcaggtgc gcgagtgtgc agcccaactg acgcgcatgg cgaaacagat
    11941 gggcatcgcc atgatactgg tcggacacgt gaccaaagac ggcgcgattg ccggcccgcg
    12001 tgtgctggaa cacatggttg ataccgtgct gtatttcgag ggcgaccaac attccaacta
    12061 ccgcatgata cgcgccatca aaaaccgctt cggcgcggca aacgaattgg gcgtgttcgc
    12121 gatgaccgaa aacggtttga aaggcgtatc caatccgtcc gccatcttcc tcgccagcta
    12181 ccgcgacgat acgcccggct cgtgcgtttt ggttacgcag gaaggcagcc gaccgctttt
    12241 ggttgaaatt caggcattgg tcgatgacgc acacggcttc acccccaaac gcctcaccgt
    12301 cggactggaa caaaaccgcc ttgcgatgct gcttgccgta ttaaaccgcc acggcggcat
    12361 cgcctgtttc gatcaggatg tgtttttaaa cgccgtcggc ggtgtgaaaa tcggcgaacc
    12421 ggcggcggat ttggcggtca tcctcgcgat gctttccagc ttccgcaacc gcccgctgcc
    12481 cgaaaaaatg gttgcatttg gagaaatcgg tttgagcggt gaagtccgcc ccgtcgcgcg
    12541 cggacaagag cggctcaagg aagcggaaaa gctcggcttt aaacgcgcca tcgttcccaa
    12601 agccaatatg ccgcgcaatg ccaaagagtt tccgaacctg aaaatctacg gcgtttcgag
    12661 tttgcaggaa gccatcgata tttgccgcga cagcagggaa taaacggaaa tgccgtctga
    12721 aatcgggttt cagacggcat ttggtttgtg gcggattgaa acaagaaggc ataccggcga
    12781 cagataagat ttgcggcaaa gttgcctgtg atgtggcaaa aacacacacg cccgtcatcc
    12841 ccgcaagggt gggaatctgg agtcgtccgt ttctgcaatg attgaaaatc acggtaaccc
    12901 aaccgattgg attcccgact tcgtgggaat gaggggcgtg tgcatttgat ttccatccgc
    12961 catatgtcgg cgacgggctt attcgcctac ggttttttgt atcagttttt cggcgtttgc
    13021 caaagtgttt gccacttcgt caaaaccgat gcggctgccg gcgatgaggg cgcgcgtatc
    13081 ggtataggcg gcgcgtactt tgccgtccgt ttcggtaacg aggacgcgca ggggcagttg
    13141 cagggcgaag gcggggtctt tgaccatcag cggcgtgccg gctttgggcg tgccgaagac
    13201 gatgactttt gccggctgca tcgttaagcc gtttcggcgg gcggcttcct gatggtcgat
    13261 gacggcaaaa atgtccatcc ctttgctttt tatggcggtt tcaaggcggc tgacggtttc
    13321 gtcaaaactg tattttgagg taagggtatg cgtggtcata gcggtttcgt tttgggtgga
    13381 cggtttgccg gcagggtgtg ccgaagcggt tgaaatgcag agtgcggatg cggcaatcag
    13441 ggggagtatg tgtttcatcg tatttccttt ttccttttcg gtttgtttat caggcttcgg
    13501 acggggcggc acgcgccgcg cctgatgttg cgccgcgtgc cggagtgccg tatgccgtct
    13561 gaaagcctgt cctttcagac ggcattgcgt catttcatcc cttttttgag caggtcttca
    13621 taaccgccgt gattggcaac atttgtataa cctgcttttt tcagttcttg aagggcggct
    13681 tcggcacgcc gtccgctgcg gcagtagagg ttgaccggcg tgtctttgtc gggcgcggct
    13741 tcgtgtatgc ggcggatgat ttggtcgacg gggatgttga ccgcgttgtg caaatgccct
    13801 tcgctaaatt cctgttcgct gcggacatcg atccaaacgg ccggatgttg cgcggtttgg
    13861 gcggcggata cgggtttttg cggggctgcc tgcgcggcaa aggcggctga ggcaatgagt
    13921 gcggcggtaa tcaggtgttt gatattcata gggttctcct gcggttgttg tccgaaagga
    13981 cgggaagtta ttttatctgt tccaaagcgg cggcatctat gtcccaacgc caaacgccgc
    14041 cgcctttgcc atccaatccg cgcaaaaaca ggtagcggac ggaaacggcg gcgggcggtt
    14101 gtccgcgcag tttgaagtag cgcgcggcgg caacggcgta aatcagtgcc tgaaggtaat
    14161 agtggtggtg cgcgacggct tcgtccatcg cctgttgcgt gtaggcggat gcgtccgtac
    14221 cgaggtggtt tgatttgtag tcgatgacgc agatattgcc gtcggggtct tggcagacca
    14281 tatcgacaaa gccgtttaaa aagccgttga cggtgtggaa gtcgagcgtt tcggcagcgg
    14341 cacggcagac ttcgggcagc ctgatgtcgt cgcgggcaaa ccagtcgcgc agccgtttga
    14401 ggctgaagtt ttcggtaaag agcgtaaagc ccatttcggg gcagcggtac tcgggtggga
    14461 tgttggacag gccgtatgcc cccgtcagcg gcgttttgcg gcaggcttcc gccatttcgg
    14521 caacggcggg cagccatatt tcttcaaaac cgtatttttt cagcttgtcg gcaatgaggg
    14581 tttcctgtcc ggcggctgct tgtccgaatt tgaaatcttc aagaatttcg tgcaggcaca
    14641 gccccgcctg cgtgcctttc gggaagttgt gtatcgatat gccgtctgaa gccgtcggcg
    14701 tttcagacgg catcgccggc accgagattt cggcggcatc caaggacggg caggcatctt
    14761 cttcgccgcc gtcgggcgtt tgggtatggc ggcttaaggc ggtaaagctg gtgtggcgga
    14821 caaatcggaa tctgcgttcg ggaatgctgt ttgcggtaaa ttcggcggtt tgaccggcgt
    14881 tgccgcgata ggcggcaggg ggcggcgcat cttctgtgaa ggcgaaattt gtgccggaag
    14941 gggcgttgtc cgccacgcgc cgccagttgc gtttgagcat cgcgatgccg tctttttcac
    15001 acgcataggc acggcggacg gtttcgcggc tgtcttgcgg caagccttca atcaggtagg
    15061 cgagggggtt gtcggcagta ttggtggagt acgcggcgta gatgttgagc tgttcctcgg
    15121 cacgcgtcag tgcgacataa agcaggcgca ggcgttccgc catttcttca tcggcgtatt
    15181 gtttctgttc gtcttccgac agttgcgcct ttgccaacag ttcggttcgg tttgcgcttt
    15241 ggtggaggat ttgccagtcg gacggcccgg tatcttgcgc gtcccacgca aacgggcagt
    15301 acaccagcgg atactgcaaa cctttcgagg cgtgcatggt aacgattttg accaaatctt
    15361 cgtcgctttc cagacggatg gcgcggttgt cgccgccgtt gttgccggcc aggctgattt
    15421 ggtcgcgcaa ccatttgtgc agcgcggctg ggttgcggtt ttgcgcgtct tcggcggcaa
    15481 gcagttcgag cagttggaaa tagttggtca ggctgcgccc gttgttccgg cttaagaggc
    15541 gcgtttcgat gccgtgtgtt tgggaaaatt gctgcatagc ggcgaaaatg ccgtattgat
    15601 tccagttgtc gagcgcggtt cgggcagatt ccgcccaatg caaaatctcg ctttcgtttt
    15661 ggttgaagtc gtgcaattgc tgcgcgtcat aaccgaatat gccgcttgtc aggacaaagc
    15721 gcagcgttcc ggcgcggcgc ggttcgagcc agaagccgat gagtgcggac agggcggcgg
    15781 cttcgggcga ggcgaacaca gattcgcgcg aaagcaggac gctttgcacc tgccgttttt
    15841 tcagggcggc ggaaaccatc accgcctcgt tgtgcgtgcg taccagcacg gcaatatcgc
    15901 ccgactgcaa cgggcagcct ttgaaattca gacggcctcc ggcggcttcg ttgagcgcgt
    15961 gggcgatttc gtcggcgcaa tagtcggcgg cacgtcggcg caaaacgtct ttgttggctt
    16021 tttcattgtc gttttcgtgc agccaacgaa cctgtacggc aggacgtttg ggcgacaatc
    16081 tgctttcggc acgcgccgca ccgacttccg aatagccgat gttttccaaa acgaacgggc
    16141 gttctttgag gcggaacagt gcgcctatgc tgccgataag cgcggcgtgg ctgcggtagt
    16201 tggtggcgag cgtgtagcgg tgccgcgcgt ctccggcggc ttgcaggtag gcgtaaatgt
    16261 ccgctccgcg aaagctgtaa atcgcctgtt tgggatcgcc gaccagaaac agcgggcggt
    16321 tttgggcgat gaagattttt tggaagattt cgtattgcag cgggtcggtg tcttggaact
    16381 cgtcgatcag cgcggtttcc cagttttcgg caacggcgcg ggcgagggtt tcggcgtgcg
    16441 gattgtcggt cagcgcggtg tggacatcga gcagcaggtc gtcgaaaccg cgttcgcggc
    16501 gcgatttttt catctcggca aggctgcggt tgaggtatcc gattaaatcc agttgcagcc
    16561 ggattattgt tgcttcttcc gcttcttcga gtgcgttcaa atcgtacccg aagtctgcca
    16621 gtttctgcaa ttcggcaaat actgccgcat cgggcgtttt gccttttttc agtctggctt
    16681 cgagtttgtc ggacgcaagt ttcaagagcc gttcgtgcgt gtctttgtcc agaaagggca
    16741 gttgtccggc ggcggatttt tgtgccagtt ctttaaaaag gttgccgaag ctgtttttgc
    16801 ggtaactgtt gccgttgagg tcgggatgga tgcgccaaaa gccggcttcc agctcgggca
    16861 gcaggcggca gacggtttgc catgaggttt cggcgttgcg ctgcgcctgt ttcaaatccg
    16921 cctgcggacg gcggaaattc aggtacgggc gggacaggta ggcgcggatt tgggcaagga
    16981 cggtttgcgg cacagctttg cgtttaaacg ccaatgcggc aagcaccgga tcattgctga
    17041 cgcgttcccg ccaaaaatct tgcgccagga taagcaggcg gtcgccgtct tcttcggtca
    17101 gttcgacatc gaacggtgct tggcacagga aggcgtagtc gcgcaggata cgttggcaga
    17161 agccgtggat ggtatagatg gcggcgttgt cgaattgccc gatggcggcc ttgaggcgga
    17221 caatcagacg cgtccggctc tctttttgca aagcctgttt taagagtgcg ggcaggaagg
    17281 tgtcgccttt gtggtgttcc gcacaatagg cggcgatgcc gtctgaaagc gtgtcgtctc
    17341 caagtttggc aatttcttta ctttctaaaa cctgcaacac atcgtccaaa cgtgcgcgca
    17401 ggcgtgtttt caactcggcg gtggcggctt tggtgaaggt aacgaccaat acgcgctcga
    17461 cgtttttttg ttccaatacg atcaggcgcg taaacagggc ggcaatgccg taggttttgc
    17521 cggtgccggc agaggcctca atcaggttgg tgccggcaat ggggacggtt agcgagtcga
    17581 atgcttggat gggtgcagac atagtgcgcg ctcggaaaac ggttggacgg tatagtggat
    17641 taacaaaaat caggacaagg cgacgaagcc gcagacagt
//
LOCUS       DAXHKX010000024        16935 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHKX010000024 DAXHKX010000000
VERSION     DAXHKX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.024"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(68..1237)
                     /gene="metK"
                     /locus_tag="ACFMWZ_000675"
     CDS             complement(68..1237)
                     /gene="metK"
                     /locus_tag="ACFMWZ_000675"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC5934670.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDSEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1420..1530)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1583..2479
                     /locus_tag="ACFMWZ_000676"
     CDS             1583..2479
                     /locus_tag="ACFMWZ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC5934671.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2523..3587)
                     /gene="tsaD"
                     /locus_tag="ACFMWZ_000677"
     CDS             complement(2523..3587)
                     /gene="tsaD"
                     /locus_tag="ACFMWZ_000677"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC5934672.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPIHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGSMTVQIPTPTGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            complement(3703..4890)
                     /gene="ccsB"
                     /locus_tag="ACFMWZ_000678"
     CDS             complement(3703..4890)
                     /gene="ccsB"
                     /locus_tag="ACFMWZ_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC5934673.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4883..6835)
                     /locus_tag="ACFMWZ_000679"
     CDS             complement(4883..6835)
                     /locus_tag="ACFMWZ_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC5934674.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQVV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7112..7735)
                     /locus_tag="ACFMWZ_000680"
     CDS             complement(7112..7735)
                     /locus_tag="ACFMWZ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC5934675.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7941..8570
                     /gene="yihA"
                     /locus_tag="ACFMWZ_000681"
     CDS             7941..8570
                     /gene="yihA"
                     /locus_tag="ACFMWZ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC5934676.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8691..11087)
                     /locus_tag="ACFMWZ_000682"
     CDS             complement(8691..11087)
                     /locus_tag="ACFMWZ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC5934677.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVQKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLEAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11239..12354
                     /gene="pilM"
                     /locus_tag="ACFMWZ_000683"
     CDS             11239..12354
                     /gene="pilM"
                     /locus_tag="ACFMWZ_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC5934678.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12357..12956
                     /locus_tag="ACFMWZ_000684"
     CDS             12357..12956
                     /locus_tag="ACFMWZ_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC5934679.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKVEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12957..13604
                     /locus_tag="ACFMWZ_000685"
     CDS             12957..13604
                     /locus_tag="ACFMWZ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC5934680.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13622..14167
                     /locus_tag="ACFMWZ_000686"
     CDS             13622..14167
                     /locus_tag="ACFMWZ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC5934681.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14186..16495
                     /gene="pilQ"
                     /locus_tag="ACFMWZ_000687"
     CDS             14186..16495
                     /gene="pilQ"
                     /locus_tag="ACFMWZ_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC5934682.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQQ
                     AAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRS
                     LDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESG
                     GVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWD
                     QALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKY
                     KNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDE
                     LDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKW
                     GAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAK
                     IESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQC
                     ASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFK
                     TRGKKTDRRELLIFITPRIMGTAGNSLRY"
BASE COUNT         4567 a         4823 c         3866 g         3679 t
ORIGIN      
        1 tgttaatcca ctatataaaa atgccgtctg aactgttcag acggcatttt tgattttcaa
       61 accggaatta cagccccgct gccgccctca atgcagcagc tttgtcggtg cgctcccaag
      121 tgaactcagg ttcttcgcgg ccgaaatgtc cgtaagcggc ggatttactg taaatcgggc
      181 gcaagaggtc gagcatttgg acgatgcctt tggggcgcag gtcgaaatgt tcgcgaacta
      241 aggtaatcag tttttcttcg ctgattttgc ctgtgccgaa agtatcgatg gaaatcgaag
      301 tcggttcggc aacgccgatg gcgtaggaaa cttggatttg gcattgggtt gccaatcctg
      361 cggcgacgat gtttttcgcc acatagcggc aggcgtaggc ggcggaacgg tcaaccttgg
      421 acgggtcttt gccggaaaac gcgccgccgc cgtgcggagc cgcgccgccg taggtatcga
      481 cgatgatttt acggccggtc aaaccgcagt cgccttgcgg gccgccgatg acgaagcggc
      541 cggtcgggtt gatcaggtat ttggtttcgt cggtcagcag ttcagacggc aggacgggct
      601 tgataatctg ctcaatgacg gcgtttttca actcttcata accgatttcc gaatcgtgtt
      661 gcgtagagag gacgacggta tcaatgcgtt ttaccttacc ggtttcgctg tcgtaaacca
      721 cggtcagttg ggctttggca tcaggacgca gccacggcag gcggccgtct ttgcgcaatt
      781 cgctttggcg ttgcatcagg cggtggctgt aatagatggc aaacggcatc agggtcgggg
      841 tttcgtcgca ggcgtagccg aacatcagac cttggtcgcc tgcgccttgg ttcaagtcga
      901 tgccttcgcc ttcgtttacg ccttgggcaa tgtccggaga ttgttggtcg tagtacacgc
      961 cgaccgcgca gccgttggca tcaaagccca gctcggagga gttgtagccg atgcgtttga
     1021 tggtttcgcg tgcgactttg atgtagtcca cttgggcggt ggtggtgatt tcgcccgcca
     1081 atacgcacaa gcctgtgttg accaaagttt ctgcggcgac acgcgctttg gggtcttgcg
     1141 ccagaatcgc atccaaaata gcatcggata cttggtcggc cactttgtcc ggatggcctt
     1201 cggataccga ttcagaagta aacagatatt cgctcattga gattttcctt gaaaaacaaa
     1261 ccatcttcgt tagacggcat gttgtatgaa cataatgtcg acagcgggaa atatagcaaa
     1321 atttccctat tcgcgccatt cagttgagaa acattcccat ttgaatggca ctttggaatc
     1381 tctgcccgta cgtttcgtac aggcaaaaaa attcccgcat caagcgggtt tggattgctc
     1441 tggtgagcca catcggcttt tcaaccgtcc accttacttt tccttttgaa aagcaggttg
     1501 gcatggaatt cccaactctt aatgcagcgc ggatggtagc agaaaagcct ttccgccgca
     1561 atacttccct ttttcagacg gcatgtttcg tttacaattc gggctgtttc cccctttgcg
     1621 aaccgccatg cacatcctgt tgaccgccct gctcaaatgc ctctccctgc tgccgctttc
     1681 ctgtctgcac acgctgggaa accggctcgg acatctggcg ttttaccttt taaaggaaga
     1741 ccgcgcgcgc atcgtcgcca atatgcgtca ggcaggcatg aatcccgacc ccaaaacagt
     1801 caaagccgtt tttgcggaaa cggcaaaagg cggtttggaa cttgcccccg cgtttttcag
     1861 aaaaccggaa gacatagaaa caatgttcaa agcggtacac ggctgggaac atgtgcagca
     1921 ggctttggac aaacacgaag ggctgctatt catcacgccg cacatcggca gctacgattt
     1981 gggcggacgc tacatcagcc agcagcttcc gttcccgctg accgccatgt acaaaccgcc
     2041 gaaaatcaaa gcgatagaca aaatcatgca ggcgggcagg gttcgcggca aaggaaaaac
     2101 cgcgcctacc agcatacaag gggtcaaaca aatcatcaaa gccctgcgtt cgggcgaagc
     2161 aaccatcgtc ctgcccgacc acgtcccctc ccctcaagaa ggcggggaag gcgtatgggt
     2221 ggatttcttc ggcaaacctg cctataccat gacgctggcg gcaaaattgg cacacgtcaa
     2281 aggcgtgaaa accctgtttt tctgctgcga acgcctgcct ggcggacaag gtttcgattt
     2341 gcacatccgc cccgtccaag gggaattgaa cggcgacaaa gcccatgatg ccgccgtgtt
     2401 caaccgcaat gccgaatatt ggatacgccg ttttccgacg cagtatctgt ttatgtacaa
     2461 ccgctacaaa atgccgtaac gaaaataaaa atgccgtctg aacaatttca gacggcattt
     2521 tgtcatttga cgatttccga cagcggccag cgcggacgga cattgaacgc accgacctcc
     2581 ctgcccttgc ccaggtgcat cgcaccggca aaggcaatca tggcaccgtt gtccgtgcag
     2641 tatgccgtcg gcgggaaaaa cacgctgact ttttcggacg gatgtttcgg cttgcctgtt
     2701 ggggtcggga tttgcaccgt catgctgccg aaagtttcac ggagtttgcg gtttgcaccg
     2761 accccgccgg cgaccactac ggttctgaac cctgtctgca acagggcttt tttcactttc
     2821 gccgccaaca catcgactac cgcatcttga aacgcacggc agatgtcgtt gcgtgtctgc
     2881 tcaggaatgt catccgcccc gttttccgcg cgcactttct cgacggcagt caaaacagcg
     2941 gttttcaaac ctgaaaaact catctgcaaa tcgtcggaat gaatcatcgg gcgcggaaaa
     3001 acaaacgctt cgggcctgcc cgattccgca agttccgaca gtttcgcacc gcccggatac
     3061 ggcaagccca gcagtttcgc cgttttgtcg aatgcctcgc ccgccgcatc atcgacgctc
     3121 tcgcccaaaa gcgcgtagtc gcctatgccc ctgaccgcca taatctgcgt atgcccgccc
     3181 gaaaccagca gcgcgacaaa aggaaagtcg ggtttttcct ccgccaacag cggcgacagc
     3241 agatgtcctt ccaaatgatg gatggggatg acgggcttgc ccaatgccaa agccagtgca
     3301 ttggcgtagc tcgaacccgc cagcagcgca ccgcccagac cgggtccctg cgtaaaggca
     3361 accgcgtcaa tgtcgccata cgatgcaccc gcctgcgcca aacagccttc cgtcaacgga
     3421 acaaggcggc ggatatggtc gcggcttgcc aattccggca caaccccacc gtattcggcg
     3481 tgcattgcca tttgagtgtg caggcagtgc gcccgcaatc ctcgttccgt atcgtaaagc
     3541 gcaacgccgg tttcgtcgca agaagactcg attcctaata ccaacatggt ctgatgccgt
     3601 taaaaactga aaaacgtatt ttagcggatt tcggcacgac tgccgtatcc caaaaacgga
     3661 acatgccgtc tgaagccctt cagacggcat cgtcgcaccg tatcaaagcg ttccgtaaga
     3721 atgcagcccg ctcaaaaaca tattcacgcc gataaaggcg aatgcggtta cgaacaaacc
     3781 gataatcgcc caccacgcca gcactttgcc gcgccaaccg gcaaccagcc gcaagtgcag
     3841 ccaaacggcg taattgagcc agacgatgaa cgcccacgtc tctttcggat cccaactcca
     3901 atagcgtccc caagcatctg ccgcccacag cgcacccaaa atggtggcaa tggtaaagaa
     3961 cagaaagccg acggcaatcg ccttatacat gacttcctca atcaacacgg attgcggcag
     4021 ccacgatttc ccgccttttc cttccgcacg cagggaaacc agttcggcaa taccgagcat
     4081 cgcggaaatg caaaacgcgc cgtaaccgat aaagtttgcc ggaacgtgga ttttcatcca
     4141 ccaagattgc aatgccggaa tcagcggctg gatggtatgc gcctcgcggg acacgctgta
     4201 ccacaagacg aatccaacca cgaccgccat aaagccgaac acgaagccgc ccaatttctg
     4261 tatggcgaac ttaccttcat aataaagata catcagcgcg gtaatcacca aaaacaggat
     4321 gaacacttca tacaggttgg aaaccggaat atgccccgca tcgggacgga gcagatagct
     4381 ttcgtgccaa cgtaccagca gaccggtaaa gcctgctacg gcagacaccc atgcaaacac
     4441 ggttcccata cccaacagcg tgttggtcgg cacatttttt acgcttgcca aaaccgcgcc
     4501 cgaaatatag gcgaacaggg cgaagaagac aaaggcgcac tgccacataa tcgccgactg
     4561 gctgctgagg aaataccgca acaggaaaat ctctgccgat ttgatgttgc cgccatacaa
     4621 accgacggcg gcataggcaa gcaatacgct taaaggaaca aaccagcgca tcggtttgaa
     4681 aaaccaaccc aaaaacacgg caataccggc acttgcccac aacatgaccg tttcgtaaat
     4741 gtccatatgc ataccggaac gggtctgtac gaaaaccgta gccgcaaaaa ccagcacggc
     4801 aaatacccaa tcccaaagat tcagattgct gatcaaagac ttctgaatca gcagctcgtg
     4861 ttccggaagg gttttatagt gttcagtcat gattcaagtc cttgccgagc cgttgcagac
     4921 tctcgacgtg ttttggaaat tccttctgca aatcccgttc actgcgggcc gaagacatgg
     4981 caaaacggat tttgccgtct gaaaacaata cccacgcccg tttttcgcgc acataaaaca
     5041 tcaataccgt acccaatacc aacagcaccg agccgagata gaccaaaagc gcacccgggg
     5101 aacgggtcat ctgcaaaccc gacgaacgca cctcggaaaa cccatcaagt tgcagcagca
     5161 taggcgcggg atattcggtc aaacccgtgt acgcatccat actgtgcagc aggaaacggt
     5221 tccgcgcttc atcctgctgc cattcgggca agccgtaccg gcgtatggtt tcatccaaag
     5281 cagcgttcat cacgccgtaa agcatttcgt agaaataacc ctgcatctta tcctgctgct
     5341 ctttcgggat attggacgta ataaattcgt ccaatcccaa atagcctttt tgtgcaaaga
     5401 tgttcagcgt gttttccgca gccagcatga attgttcgcg gatttcggca ggtgcgcctt
     5461 tggttgcgtc ggcaaccaga cgtttgcgcc cttccccatc tttcaaaaac tcacgcaatg
     5521 ccataaaggt gtccgctttc aactgcttgt ccaaggggat acgcagccag cggtattgct
     5581 gctgcaagcc gctgcgcgtg ccggtaatcc aaaaataatc ctgttcctgc aaaaccggca
     5641 gcatatagtt tttatattcg accacctgcc ctgccgcatc acggatacgg taaacaatgg
     5701 aagggccgat attggtgtat tttttacctt cctgagtaac ggcgcggaca tcgttcagcg
     5761 tggatttcag gcttttttcc cgttccgcgc cctcgctcat gtcctccaca ttcatagaag
     5821 tgaactgatc gaactcaaga cgatatttgt gtttgccaat ttccaacgga aactggtgta
     5881 tggatgttgc cttcaacacg acaggctcgc gcgaagcatc acccagattc cacgccttga
     5941 atgtcaaatc cgaaccaccg tcggcaaaac tcgcctgata aatcgtgatg ccgtgcaagg
     6001 tcaaaggatg gttcacgcgg atggtgcgct cgagtttctc accggttgcc ttgtccgtca
     6061 cttcaatatc gctggcgaaa tcacgcggca tacccgtatt gtaaaaatcg atatggaatt
     6121 ttttcagttt gacttcaaaa ggcaagtcct gaaccaatat cccgttgtcg gcattcagga
     6181 aaaccacatc cgcactctgc ccctcggaaa tattgacgtt gcccctaaat gagagattgg
     6241 acgcactcaa aatactttcg ggcttgaaat ccttggcata aaccgcctga ttgtccggaa
     6301 caatccgacc ggtcagcata cccagtttca acagcaggtt actgtctatc aacccgccca
     6361 ggcaaatgac aatcaaagca acatgggcaa agatatagcc ccatttgttc atcgtgcctt
     6421 ttttggcggc aatcagaacc gacccgccct cacggctgac ggtttttccc tgaaaaccct
     6481 gcacctccag ataacgtttg gcaacctcgg gcgcaatttt tacatccaac agcgaagaat
     6541 ggcgcatcgc cgccagagat ttttctttaa ccttttcccg aaaagacttc atttcgcgcc
     6601 agaacggcgg cacattgcga atcaggcaca aactggtaga aaccaccaaa aacatcatga
     6661 taacgacaaa ccatgccgaa gcatagacgt catacagtcc cagaaaacca aaaatctgcg
     6721 cccaaaaagg tccgaatttg accaaataat ccgtctgcgg ctggttttgc tgcaacaccg
     6781 taccgataac cgatgcaata cccagcagac tgagcaaagc gactgcaaag cgcatggagc
     6841 tgaaaaaagc gaaccacgga cgggaaagaa gtgggggaga tctacgggat ttactcattg
     6901 tgtgtttatt ccgccatcag gaatatggga aagcagaatt gggcaaacag aaaacaacgt
     6961 cccgattcta ctgtcttgat gctttttatt tcaagacaat gaagacagcc tgcatcgatt
     7021 ccaacggttg cgattgaaaa aacttatcgc agaattgcct gaagccgtct gaaaactttt
     7081 cagacggcct ctaaaacaga ctattgcgga attaacgcaa accttggata aagttggcga
     7141 ccgctttcaa atcttcttca gacatacggt ttgcaatatc ttccatgatg gtatttttac
     7201 gctgaccgga cttgtaggca ttcatctgtt caacaatata tgcctgatgc tgaccgccca
     7261 aacgcggata agcctgaatt tcgcttccgc ctcccggcat acccgcaccg ctcggaccgt
     7321 ggcaggacat acacgccggc acttttttat cgctcaaacc gccgcgatag attttcgcac
     7381 ccaattcggg attttcctta ggattggctt caccggattt gggctgctgt ttggcataga
     7441 atgcggatac gttcaaaata tcctgatcgc tcaaattcat taccaccggt ttcatcacag
     7501 ctgccgaacc gtgggtgcgt ttaccgtcgc ggatgccgat agtttgatga tagatgtaag
     7561 cagtatgctg tgccgccaaa cgcggataca tcgcaatgcc gctgttaccg tctgctgcat
     7621 ggcaagccgc acaaaccgtt gcggcaacct gtttgccttt ttccacgtct gctttgggag
     7681 aggcggaaac cgcaccggca gccaaaacaa aggccaataa agtcaatcgt ttcatggagt
     7741 gctcctgatt acagcattgg ataacgcaac aatgctcttt ttatattcaa atacgggatt
     7801 tttgacccga ttaaaaccga tgattctgta aacgtgttat tctatactaa atttacatta
     7861 aattaccact gtgtttcaca taaaaccaac cgcatatttt tgctgtcgga caaacggcgg
     7921 cggaaaacaa ggatatgccc atgaaccttt ttcaaaacgc caaattcttc acgacggtca
     7981 accaccttaa agacctgccc gacacccctc tcgaaattgc ctttgtcggc aggagcaatg
     8041 ccggaaaatc cagtgccatc aataccctga ccaaccatgt ccgtcttgcc tacgtttcaa
     8101 aaacacccgg acggacgcag catatcaact tcttcgagct gcagaacggc aattttatgg
     8161 tcgatttgcc cggctacggt tatgcccaag tccccgaagc agtacgcgca cattgggtca
     8221 atctgctcgg cgactatctg caacagcgca aacagcttat cgggctggtt ttgattatgg
     8281 atgcccgcca tcctttaaaa gaactcgaca tccgtatgct ggactttttc cacacgaccg
     8341 gcagaccggt tcacatcctg ctgtcaaaag ccgacaaatt atccaaaaac gaacagataa
     8401 aaaccctgtc ccaagtcaaa aaactgctca aaccttattc cgacaggcaa aacatcagcg
     8461 tacagctgtt ttccagcctg aaaaaacaag gtattgacga ggccaaccga actgtcggaa
     8521 gctggtttga cgcagcagat gccgccgctt cctctccaaa ggaaaactga ccccaattat
     8581 acggaaaccg tatttccccc acttgaccga ccgcaaacat ttaaaaaatt gccactgcca
     8641 aatctaaaat gccgtctgaa agacttttca gacggcattt tgcggagtct ttaaaacaga
     8701 gaatccaact gctgctgttt ggaaccagta ttactcggaa gcaccggcgt ttcctgcata
     8761 tcttggcgga cttcgtcatc cgccgcctgc cgtccgcctt ctgccgcgcc cccgtcatct
     8821 tcttttgccc gtcgggaagg ttgcggcgca ataccgctgt tgtccagcgt caagcccgga
     8881 tcggttacca tacgttcctt catatagtat tcgccattgc tgctgaccac accttcaggc
     8941 attttcatcc ccttgccctg ctttcctttc aacgcaaaac gcatatagtc cacccaaacc
     9001 ggcaccgcaa tcgtaccgcc gtagccgaca cgccccatac tcttaggttt gtcgaagccg
     9061 atatatacgg cagtaaccac atcagggtta aaaccgacaa accacgcatc cttattgtca
     9121 ttggtcgtac ccgttttacc ggcaatatcc gttcttccca acgcagctgc cccccttgcc
     9181 gtaccgacac ggaccacatc ctgcataatc ttatacataa tataggcatt gcgcggatcg
     9241 attgcctgag gcgcattttg cccggcctcc aaaggttgca tttgggcgcg caacctgccg
     9301 tctctgtcat aaatcttatc gattacgtgc gaagaaaccc tatatccgcc gttcgcaaat
     9361 acgctatatg cctccgccac tttcaacggc gttgtctcgc ccgtacctaa agccatagac
     9421 aggcttgccg gcagctcgga cgacctgaag ccgaaacgcc ggatatactg ttgcgcgtaa
     9481 ccgacaccga tagacatcaa aatacggatg gaaaccatat tcttggaagc cgtcagagcc
     9541 tgtctcaaag taatgtagcc ggaatatctg ccgtctgaat ttttaggtgt ccaaaccgaa
     9601 ccgttcggcc ctttccccgg cagggaaatc ggcgcatcgt taaccactgt ggacgcggtc
     9661 atccccttag ataatgccgc cgaatagaca aacggcttaa aggtcgaacc cggctgccgc
     9721 attgcctgaa cggcacgatt gaatgttttg ctgtgaaaat cataaccgcc gaccagcgcg
     9781 cgcacagctc cggtttttgc atccagcgaa accaaagccc cctgcagcaa cggctcttga
     9841 accaccgccc aacgcccgcc gttgtttttg acacggatga ccgcgcccct gcggatacgg
     9901 tcctccccca ttttttcatt attgaccgcg cgggccgcaa aacccaaggc gcgcctgtca
     9961 agcgtaaccc gcctgccgcc gggcagctgt atgacgacat ttttcttttt agtcacatcc
    10021 aacacaacgg cgggaaccat tttatcgacg gtatagagtc ccgacagata ctggctgaca
    10081 gtctcctcga catcttcact cttactcaaa tcgatatagt tttccgcacc gcggtagctg
    10141 ctgccgcgat cgaaattccg tagagccttg cgcaatgcct cggttgccac cttctgatga
    10201 tcagcgcgga ccgtggtata aaccttaaaa ccctgcgtat aggcatcttc accgtatttc
    10261 tcatacagtt cctgacgcac catttccgcc acatataaag cactctgatc gattttctga
    10321 acaaaccgct cgtaatgcag ttcctcattc aacgcctgat cgcgctgttg cacggtaatc
    10381 atcttctcct cgagcatatt gttcaaaata tacttctggc gcaacttggc acgttccgga
    10441 ttaacaatcg gattataggc agacggagcc ttgggcagtc ccgcaagcat ggcggcttcc
    10501 gccaaagtca aatctcggac attcttattg aaatagattt gcgcggcaga tgcaaaacca
    10561 taggcgcgct gaccgaggta aatctgattg aaatacaact cgaggatttt gtctttgctt
    10621 aaagactgct cgattttata ggcaagcaac acctcattga atttgcgtgt gaacgttttt
    10681 tcactgctca aataaaaatt tttcgccacc tgctgcgtaa tcgtactcgc acccgactgc
    10741 acgctgccgg acacgacatt gccgacggca gcgcgggcaa caccccaaac atccaccccc
    10801 caatgccggt aaaagcgttt atcctcggcg gcgataaccg cattccgcaa cacctctggg
    10861 aaatcgccga tttttgtaaa ttcgcgccgc tgctccccat acataccgat gacttcccca
    10921 tccgccgaat aaatagtcaa cggcatttta ggctggtaat gctgcaaaga atccaaagac
    10981 ggcagtttcg gatacgttac caaaatagca atggcaacca aacccactcc aaatacacaa
    11041 aacccaaaaa ccaaaccaaa acaagtcgtt aaaatctttt taatcatagc tgaataataa
    11101 tttaccatta ttggtattaa ataaagtaaa atagcaaccg atttctacaa agcacggttt
    11161 caatgtgcaa agaacaagga atccattacg gataccgaaa cggttactca ctgtacaaat
    11221 aaagcaggaa ctttcatcat gcgcttgttt aaaagcttga aaaaccctaa aaaaacagat
    11281 gccaagctcc ctaaaaaatc ttcgggactc aataaccgcg cggcaatcgg catcgatatc
    11341 gaccagcatt ccatcaaaat ggtccaattg tcaggacgta gtttaaacca aattcaattg
    11401 gaaaaatacg tcattgccaa attaccaaag aatatcattc aaggcaataa agtccaaaat
    11461 tacgatcaac ttgttacata tttgcaacaa gcctatgcca aactgggtac gtcgtgcaaa
    11521 aacatcatcg cgtccgtccc gcaaaatctg gcaaccatcg aacaattgac ctacacagac
    11581 aaagatgcag aattagacct gcaggggttc gtggagtcct ccatctccga agtcagctcg
    11641 atatcgctcg aagaagccaa ttacgactat caggtcttgt cccaatcggc cgccggcgaa
    11701 gctgtgttgg ccgtcgcatc gagaaaggat gaaatcgaac ccctgattga cgcattcaac
    11761 gccgccggta tgaaattatc cgcgcttgat gtggacattt tcggacaata caacgcctac
    11821 gcgctatgga taaaccattt cgcccccgag cttgcagccg aaaaagtcgc cattttcggc
    11881 gtatatgccg cacagaccta cgccttggtc atccaagacg gaaaaatcct atacaaacag
    11941 gaaacctccg tcagcgaaga acagctcaac caactcatcc agcgcaccta tcaggtaaca
    12001 gaagaaaaag cggaagaaat catcaactcc ccgcaaaaac cttccgatta ccaagaaagc
    12061 gtggcaaact atttcaacca gcagattacc caagaaatac aaagggtctt gcagttttat
    12121 tacaccacgc agaccgcaga cgatatgacc gacatcaagc atatcctgct gaccggggaa
    12181 gcggcgcgcc aggaaggcat cgcccaaacc gtcgcctcac aaaccaatgc agatgtacaa
    12241 tgcgtccatc ccgcgcgtta ttttgcggac aacctcaaaa cagacaaaca acaattcgaa
    12301 cttgatgcgc cgacactgac cagggcgttc ggtttggcgg tacggggatt ataattatga
    12361 acaatttaat caaaatcaac ctcctcccct acagggaaga gatgaacaag cgcaaacagc
    12421 agcagtttaa aacgctgatg tacggtgccg tgctgacggg cgttgccgcc gttgcggcaa
    12481 cctacctgtt tatcgacaat atgatcaata accagtcgga aagaaacacg ctgctggaaa
    12541 cctccatcgc acacttggat accgagctgt cggaaataca aaagctcaaa caggaaaaag
    12601 atgccttcct gattaagaaa aacaaagtcg aggagctcca gctcaaacgc ctccaagccg
    12661 caaaaatcct cgacagcctg aatgaggccg tccccggaag cacctacctg acctcgctgg
    12721 atgccgttac cgccgactct tatcggctca gcggcaggac atccagcgac aaccgcgttg
    12781 ccgccatgat gagggcgatg cccaataccg gcatattcaa gcaacccgaa ttgttaagca
    12841 tcaagaaaaa caattcgcat caagaattta cccttcaggc aacattacaa cccatcgtaa
    12901 aggcggccga atccaaagag aatccggctt cgggaaacgc acaggaggca aactgaatgg
    12961 cttctaaatc atctaaaacc aacttggatc tcaacaacct tcacctgctc aaccttcctg
    13021 ccaggctttt tatcgccctg ctggccgttg ccgccgtgct ggggctcggt tatgccggat
    13081 tgttcaaaag ccagatggaa tcccttgagg aatacgaagc aaaagaaacc gaactgaaaa
    13141 acacctacaa acagaaaagt atcgacgcgg ccagcctgaa caacctgagg gacgaacttg
    13201 cctcaatccg ctctgccttc gatatcatgt tgaaacagct gccgacagat gcagaaattc
    13261 ccaatctggt tcaagagctt catcaggcag gttcgagcaa cggcctgcgc ttggacagcg
    13321 ttatgcccca acctcccgta gatgacggcc ccatcaaaag attaccctat tccatttcca
    13381 ttaccggaaa ttacgaacag atcagccaat ttacccgcga tgtcggcagc ctctcccgaa
    13441 tcattaccct tgagtcgctg aaaatcgccc aatctccgga aaacggcggc aatcctgacg
    13501 gcaagagcag catcctgaac ctcagcgcca ttgccaccac ctaccaagca aaatccgtag
    13561 aagagcttgc cgcagaagcg gcacaaaatg ccgagcaaaa ataacttacg ttagggaaac
    13621 catgaaacac tatgccttac tcatcagctt tctggctctc tccgcgtgtt cccaaggttc
    13681 tgaggaccta aacgaatgga tggcacaaac gcgacgcgaa gccaaagcag aaatcatacc
    13741 tttccaagca cctaccctgc cggttgcgcc ggtatacagc ccgccgcagc ttacagggcc
    13801 gaacgcattc gacttccgcc gcatggaaac tgacaaaaaa ggggaaaatg cccccgacac
    13861 caagcgtatt aaagaaacgc tggaaaaatt cagtttggaa aatatgcgtt atgtcggcat
    13921 tttgaagtcc ggacagaaag tctccggctt catcgaggct gaaggttatg tctacactgt
    13981 cggtgtcggc aactatttgg gacaaaacta cggtagaatc gaaagcatta ccgacgacag
    14041 catcgtcctg aacgagctga tagaagacag cacgggcaac tgggtttccc gtaaagcaga
    14101 actgctgttg aattcttccg acaaaaacac cgaacaagcg gcagcacctg ccgcagaaca
    14161 aaattaagaa gaggattact ccattatgaa taccaaactg acaaaaatca tttctggtct
    14221 ctttgtcgca accgccgcct ttcagacagc atcggcagga aacattacag acatcaaagt
    14281 ttcctccctg cccaacaaac agaaaatcgt caaagtcagc tttgacaaag agatcgtcaa
    14341 cccgaccggc ttcgtaacct cctcaccggc ccgcatcgcc ttggactttg aacaaaccgg
    14401 catttccatg gatcaacagg tactcgaata tgccgatcct ctgttgagca aaatcagtgc
    14461 cgcacaaaac agcagccgtg cgcgtctggt tctgaatctg aacaaaccgg gccaatacaa
    14521 taccgaagta cgcgggaaca aagtttggat attcattaac gaatcggacg ataccgtgtc
    14581 cgcccccgca cgccccgccg taaaagccgc gcctgccgca ccggcaaaac aacaggctgc
    14641 cgcaccgtct accaagtccg cagtatccgt atccgaaccc tttaccccgg caaaacaaca
    14701 ggctgccgca ccgtttaccg agtccgtagt atccgtatcc gcaccgttca gcccggcaaa
    14761 acaacaggcg gcggcatcag caaaacaaca gacggcagca ccagcaaaac aacaggcggc
    14821 agcaccagca aaacaacagg cggcggcacc ggcaaaacaa accaatatcg atttccgcaa
    14881 agacggcaaa aatgccggca ttatcgaatt ggcggcattg ggctttgccg ggcagcccga
    14941 catcagccaa cagcacgacc acatcatcgt tacgctgaaa aaccataccc tgccgaccac
    15001 gctccaacgc agtttggatg tggcagactt taaaacaccg gttcaaaagg ttacgctgaa
    15061 acgcctcaat aacgacaccc agctgattat cacaacagcc ggcaactggg aactcgtcaa
    15121 caaatccgcc gcgcccggat actttacctt ccaagtcctg ccgaaaaaac aaaacctcga
    15181 gtcaggcggc gtgaacaatg cgcccaaaac tttcacaggc cggaaaatct cccttgactt
    15241 ccaagatgtc gaaatccgca ccatcctgca gattttggca aaagaatccg ggatgaacat
    15301 tgttgccagc gactccgtca acggcaaaat gaccctctcc ctcaaagacg taccttggga
    15361 tcaggctttg gatttggtta tgcaggcacg caacctcgat atgcgccaac aagggaacat
    15421 cgtcaacatc gcgccccgcg acgagctgct tgccaaagac aaagccttct tacaggcgga
    15481 aaaagacatt gccgatctgg gcgcgctgta ttcacaaaac ttccaattga aatacaaaaa
    15541 tgtggaagaa ttccgcagca tcctgcgttt ggacaatgcc gacacaaccg gaaaccgcaa
    15601 tacgcttgtc agcggcaggg gcagcgtgct gatcgatccc gccaccaata ccctgattgt
    15661 taccgatacc cgcagcgtca tcgaaaaatt ccgcaaactg attgacgaat tggacgtacc
    15721 cgcgcaacaa gtgatgattg aggcgcgtat cgtcgaagcg gcagacggct tctcgcgcga
    15781 tttgggcgtt aaattcggcg cgacaggcaa gaaaaagctg aaaaatgata caagcgcatt
    15841 cggctggggg gtaaactccg gcttcggcgg cgacgataaa tggggggccg aaaccaaaat
    15901 caacctgccg attaccgctg ccgcaaacag catttcgctg gtgcgcgcga tttcctccgg
    15961 tgccttgaat ttggaattgt ccgcatccga atcgctttca aaaaccaaaa cgcttgccaa
    16021 tccgcgcgtg ctgacccaaa accgcaaaga ggccaaaatc gaatccggtt acgaaattcc
    16081 tttcaccgta acctcaatcg cgaacggcgg cagcagcacg aacacggaac tcaaaaaagc
    16141 cgtcttgggg ctgaccgtta cgccgaacat cacgcccgac ggccaaatca ttatgaccgt
    16201 caaaatcaac aaggactcgc ctgcgcaatg tgcctccggt aatcagacga tcctgtgtat
    16261 ttcgaccaaa aacctgaata cgcaggctat ggttgaaaac ggcggcacat tgattgtcgg
    16321 cggtatttat gaagaagaca acggcaatac gctgaccaaa gtccccctgt tgggcgacat
    16381 ccccgttatc ggcaacctct ttaaaacacg cgggaaaaaa accgaccgcc gcgaactgct
    16441 gattttcatt accccgagga ttatgggtac ggccggcaac agcctgcgct attgatgcgt
    16501 caaaataagg gcatatgttt tacggcatat gccctttctt tatgcttttt gccgcgaccg
    16561 aaatgccgtc attcccgcgc aggcgggaat ccagtccgtt cagtttcggt cagtttcggt
    16621 catttccgat aaattcctgt tgcttttcat ttctggattc ccacttttgt gggaatgacg
    16681 gcggaagggg taaatcctca caacccaaag cctcgtcatt tccacaaaaa acagcaaccc
    16741 gaaacagcaa cttaaaaccc cgtcattccc gcgcaggcgg gaatctaggt ctgtcggttc
    16801 aggaacttat cggataaaac ggtttctcca accctgcgtt ctagattccc actttcgtgg
    16861 gaatgacggg atatgggttt ccgtgcggac gtgttcggat ttccgcctgc gcgggaatga
    16921 cggcgacaga tgccc
//
LOCUS       DAXHKX010000025        16455 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHKX010000025 DAXHKX010000000
VERSION     DAXHKX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.025"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            209..1273
                     /locus_tag="ACFMWZ_000688"
     CDS             209..1273
                     /locus_tag="ACFMWZ_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC5934683.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="ACFMWZ_000689"
     CDS             1414..1737
                     /locus_tag="ACFMWZ_000689"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC5934684.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="ACFMWZ_000690"
     CDS             1809..2132
                     /locus_tag="ACFMWZ_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC5934685.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="ACFMWZ_000691"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="ACFMWZ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC5934686.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="ACFMWZ_000692"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="ACFMWZ_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC5934687.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3086..3856
                     /gene="tatC"
                     /locus_tag="ACFMWZ_000693"
     CDS             3086..3856
                     /gene="tatC"
                     /locus_tag="ACFMWZ_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC5934688.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWMVIGILVCFFGMMPFAQQ
                     LYTFIANPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3909..4514
                     /locus_tag="ACFMWZ_000694"
     CDS             3909..4514
                     /locus_tag="ACFMWZ_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC5934689.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPANIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4530..4883
                     /locus_tag="ACFMWZ_000695"
     CDS             4530..4883
                     /locus_tag="ACFMWZ_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC5934690.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4873..6687
                     /locus_tag="ACFMWZ_000696"
     CDS             4873..6687
                     /locus_tag="ACFMWZ_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC5934691.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKHPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6877..7554
                     /gene="misR"
                     /locus_tag="ACFMWZ_000697"
     CDS             6877..7554
                     /gene="misR"
                     /locus_tag="ACFMWZ_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC5934692.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7568..8974
                     /gene="misS"
                     /locus_tag="ACFMWZ_000698"
     CDS             7568..8974
                     /gene="misS"
                     /locus_tag="ACFMWZ_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC5934693.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9092..11518
                     /locus_tag="ACFMWZ_000699"
     CDS             9092..11518
                     /locus_tag="ACFMWZ_000699"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC5934694.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGSADLIIAVTPPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSSDWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPIYGNILTVRYAGQTTAALPPFTTQDSLHIAQFAELED
                     PQTLNQFLHILTTLSDHNLHIGKITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11563..11808
                     /gene="rpsP"
                     /locus_tag="ACFMWZ_000700"
     CDS             11563..11808
                     /gene="rpsP"
                     /locus_tag="ACFMWZ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC5934695.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11824..12333
                     /gene="rimM"
                     /locus_tag="ACFMWZ_000701"
     CDS             11824..12333
                     /gene="rimM"
                     /locus_tag="ACFMWZ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC5934696.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12333..13082
                     /gene="trmD"
                     /locus_tag="ACFMWZ_000702"
     CDS             12333..13082
                     /gene="trmD"
                     /locus_tag="ACFMWZ_000702"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC5934697.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13098..13463
                     /gene="rplS"
                     /locus_tag="ACFMWZ_000703"
     CDS             13098..13463
                     /gene="rplS"
                     /locus_tag="ACFMWZ_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC5934698.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13784..14509
                     /locus_tag="ACFMWZ_000704"
     CDS             13784..14509
                     /locus_tag="ACFMWZ_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC5934699.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14544..15419
                     /locus_tag="ACFMWZ_000705"
     CDS             14544..15419
                     /locus_tag="ACFMWZ_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC5934700.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15496..16410
                     /locus_tag="ACFMWZ_000706"
     CDS             15496..16410
                     /locus_tag="ACFMWZ_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC5934701.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4277 a         5272 c         3612 g         3294 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacgg catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 tatccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgctggccgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagtaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaagtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca acttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagaact
     1081 ggatgtgcgc ggcacgattg cctactgcaa cgaccacgcg gaaaccatca aactggttga
     1141 agaaggcaaa atcaacctcg agcctttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcaacg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctacga agacggcgaa atggtttgtt tcaaagacat caaccccgcc gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac tcaaatcgaa ctggaagaac tgaggaaggc caagcaggaa
     2581 ttcgaagctg ccgccgctca ggttcgagac agcctcaaag aaatcggtac ggatatggag
     2641 ggtaatctgc acgacatttc caatggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgccg atttcggtgt cgatgaaaac ggcaatcccc ttcccgatgc ggcaaacacc
     2761 ctatcagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
     2821 cttggggacg acaggcaaac cggcagtaca gccgaacccg cggaaaccga caaagaccgc
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg ataccgctgt tgaaacccct gttccgcata ccacttcgct gcgtaaacag
     3001 gcaataagcc gcaaacgcga tttgcgtccc aaatcccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc
     3121 gcttgtcgag catctcatcg agctgcgccg ccgcctgatg tggatggtta tcggcattct
     3181 cgtctgcttt ttcggcatga tgccgtttgc ccaacaactc tatactttta tcgccaaccc
     3241 gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccgtt
     3301 tttcgtgccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct
     3361 ctaccaaatc tgggcatttg tcgcccccgc actctaccaa aacgaaaaac gcctgattac
     3421 gccgctcgtc ctctccagcg tcagcctgtt tttcatcggt atggcatttg cctacttttt
     3481 ggttttcccc gtcattttca aattccttgc cggcgttacc cctgtcgggg tcaatatggc
     3541 gacagacatc gacaaatacc tctccttcat cttggggatg tttgtcgcat tcggtacaac
     3601 gtttgaagtc cccattgtcg ttatcctgtt aaccaaaatt ggtgtggtaa caaccggaca
     3661 gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac
     3721 gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc
     3781 aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacagcc
     3841 gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag
     3901 gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga
     3961 cacagttggg ctatcaggtc gtcaagctgc ctgccaacat cgacgaaacg gtcagacaga
     4021 acgaagaccc tgcccgttac gttcaaagga tggcagaaga aaaaaaccga accgccctga
     4081 ccctcttttg cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct
     4141 gcgtcgtttc agacggcatc atattaggca aaccccgctc ccaagccgaa gcaatcgaat
     4201 ttttaaaccg attgtccggc aaacaacata ccgtcctgac tgctgtctgc attcattatc
     4261 gcggcaaaac gtcaagccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg
     4321 aagaaatttc cgcctatgtg caaagcggcg aaccgatgga caaagccggc gcctacgccg
     4381 tacaaggcat aggcggcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg
     4441 ggctgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgt accccacctt
     4501 tcatccgagc ctaacccgta aagaccgccg tgaacagaca aaccgcttac ctccttacct
     4561 ctttcagcct gatcgcactg ataatcctgt ccctttcctg ggaactgtgg attgcaccgt
     4621 tgcgcccggg cggctcgtgg ctcgcgctca aagccctccc cctctgtctg ccgctttcag
     4681 gcatcttgaa aaagaaaatc tatacctacc aatacagctc catgctggtt ctgatttact
     4741 ttgccgaagc cgtcatgcgc ctgttcgacg cctatcccgc agaacagatt tgcgccgcgc
     4801 tttccgcagt attcagcatc atcttcttca tatcctgcct gtcgttcgtc aaacaataca
     4861 aggaaacaaa caatgcccgc tgaaacgacc gtatccggcg cgcaccccgc cgccaaactg
     4921 ccgatttaca tcctgccctg cttcctttgg ataggcatcg tcccctttac cttcgcgctc
     4981 aaactgaaac cgtcgcccga cttttaccac gatgccgccg ccgcagccgg cctgattgtc
     5041 ctgttgttcc tcacggcagg aaaaaagctg tttgatgtca aaatcccacc catcagcttc
     5101 cttctgtttg caatggcggc gttttggtat cttcaggcac gcctgatgaa cctgatttac
     5161 cccggtatga acgacatcgt ctcttggatt ttcatcttgc tcgccgtcag cgcgtgggcc
     5221 tgccggagct tggtcgcaca ctacggacaa gaacgcatcg ttaccctgtt tgcctggtca
     5281 ctgcttatcg gctccctgct tcaatcctgt atcgtcgtca tccagtttgc cggctgggaa
     5341 gacacccctc tgtttcaaaa catcatcgtt tacagcgggc aaggcgtaat cggacacatc
     5401 gggcagcgca acaacctcgg acactacctc atgtggggca tactcgcctc cgcctacctc
     5461 aacggacaac gaaaaatccc cgccgccctc ggcgtaatct gcctgattat gcagaccgcc
     5521 gttttaggtt tggtcaattc gcgcaccatc ttgacctaca tagccgccat cgccctcatc
     5581 cttcccttct ggtatttccg ttcggacaaa tccaacaggc ggacgatgct cggcatagcc
     5641 gcagccgtat tccttaccgc gctgttccaa ttttccatga acaccattct ggaaaccttt
     5701 accggcatcc gctacgaaac tgccgtcgaa cgcgttgcca acggcggttt cacagacttg
     5761 ccgcgccaaa tcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg
     5821 cacggctgga acagttttgc ccaacaaacc ttcctcatca atgccgaaca gcacaacata
     5881 cacgacaacc tcctcagcaa cttgttcacc cattcccaca acatcgtcct ccaactcctt
     5941 gcagagatgg gaatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc
     6001 gggctgctga aacaccccct gacccccgca tcgcttttcc tgatctgcac gcttgccgtc
     6061 agtatgtgcc acagtatgct cgaatatcct ttgtggtatg tctatttcct catccccttc
     6121 ggactgatgc tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc
     6181 gccaatctcg gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac
     6241 tggacataca cccggctggt taacgccttt tcccccgcca ctgacgacag tgccaaaacc
     6301 ctcaaccgga aaatcaacga gttgcgctat atttccgcaa acagtccgat gctgtccttt
     6361 tatgccgact tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg
     6421 gcggaagaag caaccctcaa agcactaaaa taccgcccct actccgccac ctaccgcatc
     6481 gccctctacc tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcgaca
     6541 cagtcctatt acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta
     6601 tgggcgccgc tgttgcccga actgctcaaa gactgcaaag ccttcgccgc cgcgcccggc
     6661 catccggaag caaaaccctg caaatgaccc gcgccggcgg atacggatac cgcccgaaat
     6721 gtaaagctcc atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa
     6781 aaaacattgc agccgtgtta agataaaccg tcaaacaatc ttttcacgcc ccgcccgaaa
     6841 cagggtcggg gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat
     6901 gacgatgccc tgctaaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc
     6961 cgcagcgttc ccgacgggga agcaggcgta caggaaatcc tgagcgggca gtacgatgta
     7021 gtcgtattgg attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc
     7081 gcccgaagca ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc
     7141 atcggcttgg aaatgggcgc ggacgactat gtccccaaac cctgcacacc acgcgaactc
     7201 ttggcacgca tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca gaacaacgca
     7261 cccaacagca tctccgtcag cgatgtcgtc ctgtatcccg ccaaacgcca ggcatccgtc
     7321 aaagacatgc cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc
     7381 catgccggac aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaagctg
     7441 gcaaaattcg accgcagtat cgacgtacac atctccagca tccgccacaa gttgggcgat
     7501 gcctctctga ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaaataaa
     7561 cagataaatg aaactgttcc aacgcatctt cgccacattt tgcgcggtta tcgtctgtgc
     7621 aatctttgtg gcgagttttt ctttctggct ggtgcagaac acccttgccg aaaaccagtt
     7681 caaccaacgc cgcaccatcg aaaccacttt gatgggcagc atcatttccg cattccgggc
     7741 acgcggggac gcgggtgcgc gcgaaatcct gacggaatgg aaagacagcc ccgtctcatc
     7801 gggcgtgtac gttatacagg gcgacgagaa aaaagatatc ctgaaccggt atatcgacag
     7861 ctataccatc gaacgcgccc ggcttttcgc cgccggacac ccgcattcca acctcgtcca
     7921 tatcgaatac gaccgcttcg gcgaagaata cctgttcttc accaaagact gggacaaact
     7981 ccaagcccgc cgcctgccca gccccctgtt gatccccggc ctgccgctcg ccccgatttg
     8041 gcacgaactc atcatattgt ccttcatcat catcgtcgga ctgctgatgg catatatcct
     8101 cgccggcaac attgccaaac ccatcagaat cttaggcaac ggcatggaca gggtggcaaa
     8161 cggagaactt gaaacccgta tctcccaaca ggtcgacgac cgcgacgacg aattgtccca
     8221 tcttgccatc caattcgaca aaatggtgga aaaactcgaa aaactcgttg ccaaagaacg
     8281 ccacctgctc catcacgtct cccatgaaat gcgttctccc cttgcgcgca tgcaggcaat
     8341 tgtcggactg attcaggcgc agccccaaaa acaggagcaa tatctcaaac ggctggaagg
     8401 cgaactgacc cgcatggata cgctggccgg ggaactgtta accctgtccc gtctcgaaac
     8461 ttccaatatg gctttggaaa aagaaagcct gaaactcctg cccttcctgg gcaacctggt
     8521 agaagacaat caaagcattg cccagaaaaa cggacaaacg gttaccctgt ctgccgacgg
     8581 aaaaatcccc gaaaatacaa ccatccttgc caacgaaagc tacctgtacc gcgccttcga
     8641 caacgtcatc cgcaacgccg tcaactacag tcccgaaggc agcaccatcc tgatcaacat
     8701 cggacaagac cacaaacact ggataatcga cgttaccgac aacggccctg gcgtggacga
     8761 aatgcagctc ccgcacatct tcaccgcttt ctaccgtgca gactccagtg cgaacaaacc
     8821 cggaacagga ctggggcttg cattgaccca acatattatt gaacagcact gcggaaaaat
     8881 catcgccgaa aacatcaaac cgaacggtct gcggatgcgc ttcatcctgc ccaagaaaaa
     8941 aaccggttcc aaaacagaaa aaagtgcgaa ctgaccataa tgccgtctga accggcttca
     9001 gacggcattg cacaaacagt taccccaaaa caaacacaca gcgggtgcat ctccaacaat
     9061 cacgcacata ccgtccgcaa aggaatatat catgtcggca caaaccgatc cgggctactt
     9121 tttcatgccc aaccacatta ttctgatagg cgcgagcgaa cgtccgtaca gcctgggcga
     9181 acgcgtactc agcaacctgc tgagtacgcc ctttcaagga aaaatcaccc ccgtaaaccc
     9241 gcgccaccac accatagccg ggcttcccgc ctacaccagc ctcaacaaaa tccccggcag
     9301 tgcagacctg attattgccg ttaccccgcc cgacagttac gacaccctct tcaaaacctg
     9361 ccgcaaaaag cagctccgac acatcatcct catacaggac tgggacagcc tgtctgccgc
     9421 agaactgcac accgccgaaa ctgccatccg caaacaccac ggcaacggac tcaacatcac
     9481 cgcctgcacc accgcaggca tccaactgcc ctcactcgga cttaacatca gtacccaaga
     9541 cggatacgcc gcaggccata ccgccatact gaccggcaat gccgccgtca gccgccaaat
     9601 cgacaacatc ctgaacaaac tccgtcaagg cacatctcgc cacatcagcc tgcatcccgg
     9661 catcagcccc atcacttcct cagactggct caaccgcttc ggccacagcc tgcacaccaa
     9721 aaccgccgtc ctacaccaca accctgaaga ggatcagcgc aaactgttca gcgcaatccg
     9781 ccaatttacc cgccatacgc cgctgattct ccacatcacc tgccgcacga cagaaaccga
     9841 ccgtgccgta ctgcactgcc tcgcccgaca ctgcaacttc ctcatcagtt tcaacgccga
     9901 cgaccttgaa gccgcactgc gcgcccaact gtccgacctt ccacccctgt cccgactcga
     9961 catcctgtcc gacacgcctg ccgaatggct gaacgcgcac gcgccaaaaa ccctcgccct
    10021 ccactttccc aaccttcccc accacatccg caacggacac ctgaccggca cacccacacc
    10081 ctcaatctgc cacgacatcg cctcacgtca gcttgcccgc cccgacaccc aagccgtcct
    10141 gaccatcctc agcccctccg gacacgagga ttacaaaaaa acagcacgcg cccttatccg
    10201 cctgtccgaa cagaccgcca aacccctgct cgtcagcagc cccttttcag acggcataac
    10261 ccatttcgac acccccactc aggcaatccg caccctttcc taccgcaaca ccgccgccgc
    10321 cctgaaacag gcacagctcg acattgcacc gccgcagcca tgccgtctga aaacaccgca
    10381 accccaaaac atcaaaaaag cccttgcagc ggcaaacccc tccctgctcg ccgaagccct
    10441 gcacctcccc ccctaccggc acaccaccca taacgccgta caattccaat tcgacagcca
    10501 ccccatttac ggcaatattc tgaccgtacg gtacgcaggg caaaccacgg ccgcccttcc
    10561 cccgtttacc acacaggaca gcctccatat cgcccagttt gccgaattgg aagacccgca
    10621 aacccttaac cagttccttc acatactgac caccctttcc gaccacaacc tgcacatcgg
    10681 caaaatcacc ctcaacctaa acggcggaca atacagcagc gatttcatct taaagacacc
    10741 cgaaacacac gacacgccca aacgcaagaa cacaggcaaa gccgcccaaa ccctcgaaca
    10801 tgccgccgca aaaatgcaga gtgccgccgc atacctgaaa cacaaaaacc cgacagccgc
    10861 cgaatttctc cgccacacaa gcgaagccgc cgcagaactg ctcggcagca aaaccgaaac
    10921 cggagcagcc gtacccaacg tacttgcccc ctatcccgca gcatacccca aaacactgtc
    10981 cctaagaaac aacacgaccg ttaccattac ccccattttg cccgaagacg cagaagccaa
    11041 acagcagttc gtccgcagcc tcggtcccga agcacggtac acacgcttca tgacccacac
    11101 caacgaactg cccgcagcca cgttggcacg cctgtgcaac cccgattacc actgtgaagc
    11161 cgcatggacg gcaaaggatg ccgacagcaa catcgtcgcc gtcgtccgcc acagccgcct
    11221 gaatcgcaac gaatgcgaat tcggcatcac actggcggaa catatgcgcg gcagcggact
    11281 ggcacagaaa atgatggaac tcatcatcca aaccgccgca cagcaaggct accggactat
    11341 gagtgccgac attctcaaaa ccaatacccc catgatcaaa cttgctgaaa aatcaggatt
    11401 taccctcaag gaatcggaca ccgaaaaaaa cctgtaccgc gcatatctga accttgcggc
    11461 agacaaaaca acagaaaaaa caaataaaaa cttgcgcacc gaccacaaaa taacctaaaa
    11521 tcggcagttt ccatatatcc gattttctca aaaggactca aaatggtagt tatccgtttg
    11581 gcacgcggcg gctcgaaaca ccgccccttc tacaacgtca tcgttactga ctcacgcagc
    11641 cgccgcgacg gccgcttcat cgaacgcgta ggcttctaca accccgtagc caacgaaaaa
    11701 caagagcgcg tccgcctcaa tgcagaccgc ctgaaccact ggattgcaca aggcgcgcaa
    11761 gtcagcgact ccgttgcaaa actgattaaa gaacaaaaag ccgcctaatc cgcatttgcc
    11821 gccatgacag acactcaaaa ccgggtagcc atgggctaca tcaaaggcgt attcggcata
    11881 aaaggctggt tgaaaattgc cgccaacacc gaatattccg acagcctttt ggactacccc
    11941 gagtggcatt tggtcaagga cggcaaaacc atcagcgtta cccttgaagc cggaaaagtc
    12001 gtcaacggcg aactccaagt caaattcgaa ggcataaacg accgcgactt ggcattctca
    12061 ttgcgcggtt acaccatcga aataccccgt gaagcattcg ccccgacaga agaagacgaa
    12121 tactactgga cagacttggt cggcatgacc gtcgtcaaca aagaccatac cgttttaggc
    12181 aaggtaagca acctgatgga aaccggcgca aacgacgtat tgatgattga cggagaacac
    12241 gggcagattc tgattccgtt cgtttcccaa tatatcgaaa ccgtcgatac cggcagcaag
    12301 attattactg ccgactgggg tttggactac tgatgcttat ccaggcagtt accattttcc
    12361 ccgaaatgtt cgacagcatt acccgctacg gcgtaacggg acgcgcgaac agacagggaa
    12421 tctggcagtt tgaagcagtc aatccccgaa agtttgccga caacagattg ggctacatcg
    12481 acgaccgccc gttcggcggc ggcccgggaa tgattatgat ggctccgccg cttcatgcag
    12541 caatagaaca cgccaaagca caatcttccc aagctgcaaa agtcatttac ctcagccccc
    12601 aaggaaaacc gctgacacac ccaaaagcgg cagaactggc agaacttccg catctgattc
    12661 tgctgtgcgg acgctatgag ggaatagacg aaagactgct gcaaaccagc gtcgatgaag
    12721 aaatcagcat cggagacttc gttgtttccg gcggagagct tcccgccatg atgctgatgg
    12781 atgcggtatt gaggctcgta cccggcgtat tgggcgatat gcagtctgcc gaacaagatt
    12841 cgttctcaag cggcattttg gactgccccc actacaccaa acccttagaa tttcaaggca
    12901 tggctgttcc ggaagtatta cgttccggca atcatggctt gatagcggaa tggcggttgg
    12961 aacaatcgct gcgccgcacc ttggagcgca gacccgatct tttggaaaag cgcgttttaa
    13021 tcccaaagga atcccgcctc ttagaaacca tccggcaaga gcaacgggaa atccaatcat
    13081 aatttaggaa aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc
    13141 tgaataaaga aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg
    13201 tggaaggtac ccgcagccgt ctgcaagcct acgaaggcgt ggttattgcc cgtcgcaacc
    13261 gtggtctgaa cagcaacttc atcgtccgca aaatctccag cggcgaaggt gttgaacgta
    13321 ctttccaact gtactctccg accgtcgaaa aaatcgaagt caaacgccgt ggcgacgtac
    13381 gccgtgccaa actgtactac ctgcgcggcc tgaccggtaa agctgcacgc atcaaagaaa
    13441 aactgcctgc acgcaaaggt tgattcaaac cgttttcccc caatgccgtc tgaaccttca
    13501 gacggcattt cttattgctg cgatgccggc aagtcaacga tatgcctgcc cctcatccac
    13561 cttgattaac aatcactccc gcctattcaa gcataattta cagaccaaac gttatacagt
    13621 atcatatcag ccatataacg ataaacgctt taccgctttc cctttcaaaa ccgcaaatca
    13681 tcatgacacc ttccctttta ctatcaggat tgaccttccg cctcatcctt gccctgattg
    13741 ccgtatccct tttatggggc gtttactttt gggcagtatc cgcatgagca tcattgtcga
    13801 aaacctgacc gtcagctacc gccgccgacc tgccgtgcac catgtggaca ttacttttga
    13861 agaacatagt atgtgggcgg ttttcggtcc caacggcgca gggaaatcca cctttctcaa
    13921 atccttgatg ggattgcagc ctatcgatac aggcagcatc cggctggatg gattgagccg
    13981 ccaagacatc gcctaccttc cccagcagtc cgatatcgac cgctcccaac ctatgactgt
    14041 tttcgacttg gcggcaatgg ggctttggta tgaaatcggc tttttcaaag ggataaatac
    14101 cgctcaaaaa caacgcgttc acgaagcctt ggagcgtgta ggaatgcggc agtttgcacg
    14161 ccgccaaatc gcccacctct caaacggaca attccaacgc gtcctttttg cccgaatgct
    14221 ggttcaaaat gcaaaattcc tgcttttgga cgaacctttc aacgccgttg atgcacggac
    14281 gacctacgaa cttctcgacg tattgcagaa atgccattgc ggcggacacg ctatcattgc
    14341 cgtactgcac gattacgaac aagtccgtgc ctactttccc agtaccctgc tgctcgcccg
    14401 agaaaaaatt gcggcaggcg caaccgagac cattctgaca gaaccctacc tcgcccaagc
    14461 caacgccaaa atgcagcaac aggaaagccc cgactggtgc gcctcataaa tactgcccga
    14521 aaccacgccc cacaccagac accatgaatc tctacgacct gctccttgcc ccctttgcag
    14581 aattcgactt tatgcgctac gccctcgcat ccgtcttctg cctgtccctc agtgccgcac
    14641 ccgtcggcgt attcctcgtc atgcgccgta tgagcctgat aggcgacgca ttgagccacg
    14701 ccgtcctgcc cggtgccgcc gtcggctaca tgtttgccgg cttgagcctg cccgccatgg
    14761 gtttgggcgg cgtagccgca ggcatgctga tggcactgct tgccggactc gtcagccgct
    14821 tcaccaccct gaaagaagat gccaactttg ctgcctttta tctcagcagc ctcgccatcg
    14881 gcgtagtcct cgtcagcaaa aacgggagca gcgtcgattt gctccacctc cttttcggct
    14941 ctgtacttgc cgtcgatatt cctgccctgc agctcatcgc cgccgtctcc agcctcacgc
    15001 tcattaccct tgccgtcatc taccgcccgc tcgtactcga aagcatcgac cccctgtttc
    15061 tcaaatccgt cggcggcaaa ggcgggcttt ggcacgtcct ctttctcgtc ctggtcgtca
    15121 tgaacctcgt atccggcttt caagccctcg gcacactcat gtccgtcgga ctcatgatgc
    15181 tgccagccat taccgcccgc ctgtgggcga agcacatggg cgcactcatc ctcctatccg
    15241 ttctgacagc cctgctgtgc ggcttgagcg gactgctcat ttcctaccac atcgaaattc
    15301 cctccggtcc cgccatcatc ctctgttgca gcgtccttta tctcttttcc gtcatactcg
    15361 gcaaagaagg cggcattctg accaaatggc tcaaaaacca ccgccaccac accacctgac
    15421 cccgcaagcc gcttgaacaa acaggcagct tgttttacca taacagatgt tacgttatat
    15481 cacaggagaa cagttatgaa acacctcaaa ctcaccctta ttgccgcatt gctaaccacc
    15541 gccgcaactg ccgcccccct gcccgtcgta accagcttca gcattttagg cgacgttgcc
    15601 aagcaaatcg gcggagagcg cgtatccata caaagtttgg tcggagccaa ccaagatact
    15661 catgcctatc acatgaccag tagcgacatt aaaaaaatcc gcagtgcaaa actcgtcctg
    15721 ctcaacggct taggacttga agctgccgat gtacaacgtg ccgtcaaaca gagcaaagta
    15781 tcctatgccg aagcgaccaa aggcatccaa cccctcaaag ccgaagaaga aggcggacac
    15841 catcatgacc acgaccatga ccacgaagga caccaccatg accacggcga atttgacccg
    15901 cacgtctgga acgaccccgt ccttatgtcc gcctatgccc aaaacgtcgc cgaagccctg
    15961 ataaaggccg accccgaagg caaagtttat tatcaacaac gcttgggcaa ctaccaaatg
    16021 cagcttaaaa aactgcacag cgacgcacaa gccgcattta acgccgtccc tgccgccaaa
    16081 cgcaaagtcc tgaccgggca cgatgccttt tcctacatgg gcaaacgtta ccatattgaa
    16141 ttcatcgccc cgcaaggtgt gagcagcgaa gccgagcctt cagccaaaca agtcgccgcc
    16201 atcatccgac aaatcaaacg cgaaggcatc aaagccgtct ttaccgaaaa catcaaggac
    16261 acccgtatgg ttgaccgtat cgccaaagaa accggtgtca acgtcagcgg caaactgtat
    16321 tccgacgcac tcggcaacgc gcccgcagac acctacatcg gaatgtaccg ccacaacatc
    16381 aaagccttga ccaacgcgat gaagcaataa aaccgtttga ttggtttgaa tttgaaaagg
    16441 caaaaggccg tctga
//
LOCUS       DAXHKX010000026        16238 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHKX010000026 DAXHKX010000000
VERSION     DAXHKX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16238
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.026"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(49..384)
                     /locus_tag="ACFMWZ_000707"
     CDS             complement(49..384)
                     /locus_tag="ACFMWZ_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC5934702.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(465..2597)
                     /gene="dnaX"
                     /locus_tag="ACFMWZ_000708"
     CDS             complement(465..2597)
                     /gene="dnaX"
                     /locus_tag="ACFMWZ_000708"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC5934703.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREILENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAATSCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEAETAQTSVQTASAAAMPSEGKTAEPVTNQENNDVPPWEDAPDEAQTAAGTT
                     AQTSAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETET
                     FAHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGN
                     NTPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARA
                     TADKKRLDKICDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLE
                     ADPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1369..1408)
                     /gene="dnaX"
                     /locus_tag="ACFMWZ_000708"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2692..4668)
                     /gene="mutL"
                     /locus_tag="ACFMWZ_000709"
     CDS             complement(2692..4668)
                     /gene="mutL"
                     /locus_tag="ACFMWZ_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC5934704.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSVSNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQRLLIPVTFAASHEECAALADHAEALAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLNEFAQVGSSQTIEEHENHILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4811..4996
                     /locus_tag="ACFMWZ_000710"
     CDS             4811..4996
                     /locus_tag="ACFMWZ_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC5934705.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5007..5675
                     /locus_tag="ACFMWZ_000711"
     CDS             5007..5675
                     /locus_tag="ACFMWZ_000711"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC5934706.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            5675..6391
                     /locus_tag="ACFMWZ_000712"
     CDS             5675..6391
                     /locus_tag="ACFMWZ_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC5934707.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGRIVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFAAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRVRMGNCESVIEIGRRYANRFKGTPAAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQTYPGSPAAKRAAAAVRKR"
     gene            6431..6916
                     /gene="purE"
                     /locus_tag="ACFMWZ_000713"
     CDS             6431..6916
                     /gene="purE"
                     /locus_tag="ACFMWZ_000713"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC5934708.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEACVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7099..8553)
                     /locus_tag="ACFMWZ_000714"
     CDS             complement(7099..8553)
                     /locus_tag="ACFMWZ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC5934709.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8550..9251)
                     /locus_tag="ACFMWZ_000715"
     CDS             complement(8550..9251)
                     /locus_tag="ACFMWZ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC5934710.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQLARAALAGSNIE
                     PIAFEREIDTWKTEFFANIDAGFSSSQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9248..10027)
                     /locus_tag="ACFMWZ_000716"
     CDS             complement(9248..10027)
                     /locus_tag="ACFMWZ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC5934711.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            10311..11717
                     /locus_tag="ACFMWZ_000717"
     CDS             10311..11717
                     /locus_tag="ACFMWZ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC5934712.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADM
                     NESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLSISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            11829..13355
                     /locus_tag="ACFMWZ_000718"
     CDS             11829..13355
                     /locus_tag="ACFMWZ_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC5934713.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            13410..13943
                     /locus_tag="ACFMWZ_000719"
     CDS             13410..13943
                     /locus_tag="ACFMWZ_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC5934714.1"
                     /translation="MFPPDKPLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(13986..15287)
                     /gene="aroA"
                     /locus_tag="ACFMWZ_000720"
     CDS             complement(13986..15287)
                     /gene="aroA"
                     /locus_tag="ACFMWZ_000720"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC5934715.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNTCEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     ENYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVCVIGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            15682..16158
                     /gene="greA"
                     /locus_tag="ACFMWZ_000721"
     CDS             15682..16158
                     /gene="greA"
                     /locus_tag="ACFMWZ_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC5934716.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
BASE COUNT         3389 a         4388 c         4578 g         3883 t
ORIGIN      
        1 agattctacg ttctagattc ccgcctgcgt gggaatgacg gtcgggtatc agcggaagaa
       61 gtcgcccact ccggggggta gaccttgcgt gaatgcgccc attgttttgt ttgcggtttc
      121 ttcggctttg ccccgggcgg atttgagggc ggcgaggatg aggtcttcga gcatttcttt
      181 gtcgtcggcg gcttcttgaa tcaaatcggg gctgatgtcg attttgcgta cttcgtgcgc
      241 gcaggtcatt gtgattttga ccaggccgtt gcctgcttcg ccttcgattt cggtttcggc
      301 aagtttggct tgcgcttttt tcatattttc ctgcatttgc tgcgcctgtt tcatcaggcc
      361 gcctaatccg gcttttccga acatactgaa tactcctgtt tgtgtttgaa tgaatgaggg
      421 aaacggcaat gccgtccgaa gggttcgggc ggcattatat ctgtttatgg ccggtttgct
      481 gccaattcca gtgattcggg ctgccattgc gcgccgaatg cttggaggat tttttgtgcg
      541 gcggggtcgg cttcgagcaa tgcttgtgct ttttgcctgt cttcggcttg gacgcgcttg
      601 tcctgcatcg cgggggtttc cctgccggct tcgtcgcgcc agtcttgggt ttgcagggtg
      661 agttgcagtc cgtaggcttg ggcaagggtg tcgcagattt tgtcgagacg ttttttgtcg
      721 gcggtggcgc gggcttcggc ggtcatcgcc aaaaccatca ggccggtgtc gggatggtat
      781 tccgtccacg cggagtgttg cgccggcatt tgcgccgcgc cgagtttgcg ggcgaaatgc
      841 cggacgatgg ctgcccagtt ttcggtggaa aattcgggcg gcggggcgga cggcgtgttg
      901 tttccgccga tgccgcctgc ttctgcttct tcgtccgcgc ctccgcctgc ggtatcggca
      961 ggggcggcgt gttcccaatc gggcggcggg atttctgcgc cgtcttctac ggggcagtca
     1021 ttatccggaa agccgtaacc gtagaaaggt tctgcagggg cttcgtgtgc aaatgtttcc
     1081 gtttcaacgg cttcatcatt cggtgttgcc tgaatggggt tttcagacgg cacttcggac
     1141 aagggggcat cggttacgtt gtcggctgcc ttgttcttgg aaacttggtt ttcgggcggc
     1201 gtttcggctt cggatgccgt ctgaatgctt tttgccgacg tttgcgcggt cgtgccggct
     1261 gcggtttggg cttcgtccgg cgcgtcttcc caaggcggaa catcgttgtt ttcttgattg
     1321 gtaacgggtt cggcagtttt gccttcagac ggcattgccg ctgcggatgc cgtctgaacg
     1381 gatgtttggg cggtttccgc ttcagggcgc ggttggggct tttttgcggc ggtttccttt
     1441 tcggcggttt gtgccgatgg ggattgaagt tcggtatttt caatcacggc atttgcatca
     1501 cacgatgttg ccgccaaggg cgcaaacgcc agcatccgca gcagggtcat cataaagccg
     1561 gcgtattcgt cgggggcgag gctcaggtcg cgtttgccgt ggacggcgat ttggtagtaa
     1621 agctggattt gttcgccgct tatggtttgg gcgaggcggt gcaaaatatc ggaatcgggg
     1681 tcgtcgtgcg ccaaggcatt cggcactgcc tgtatcaggg cgaggtgttg cagcagtatg
     1741 gcgagttcgc ccaaggcgtt gtcaaagccg acggcacacg ccgccatttc ctgcgctttg
     1801 gcggtcaggg ctgcgccgtc ttggttgatg atgcctgtca gcagttcgta aaggtattgt
     1861 ttgtcaaccg cgccgatcat ttggcggaca tcgttttcgg caactttgcc cgaacccagg
     1921 gcgatggctt ggtcgagcag gcttaaggca tcgcgcatcg atccggcggc ggcacgtccc
     1981 aaaagttgca gggcggcggg ttcgtaggcg attttttcgc tgtcgaggac gtgggcgagg
     2041 tggtcggcaa cctgctgcgc ggtcatattg cgtaagacga attgcaggca gcggcttaat
     2101 acggtaacgg ggactttgtg cggatcggtg gtcgcgagga tgaatttgac gtgttcgggc
     2161 ggctcttcca gcgttttgag catagcgttg aacgcgcttt tggaaagcat atgcacttca
     2221 tcgatgatat agactttgta tttgccggca gtcggcgcgt attgggcgtt ttccaagatt
     2281 tcgcggatgt tgtcgatgcc tgtgttggag gcggcgtcga tttcgagcag gtcgacatag
     2341 cgtccggcat cgatttgggt gcagctttgg cacacgccgc aaggttcgcc gtgttgcgcg
     2401 ttttcgcagt tgaggctttt ggcaaggatg cgggcgatgg tggttttgcc cacgccgcgc
     2461 gtaccggtca gcaggtaggc gtggtgcagc ctgccttcgt ccaaggcatt ttgcagggct
     2521 ttgacgacgt gttcctgacc gactaagtcg gcaaaggttt tgggccgcca ttttcgggcg
     2581 agaacttgat aggccatatt tttctcttgg tttcggtcgt gatgtttctg tcggtgcgtc
     2641 ggaatgccgt ctgaacggcg gcttcgggcg gcgtattcta gcactttcgg cttactgtcc
     2701 gcgcaagaac agtgcgtcca attctttcaa agtcagtttg acccaagtcg gcctgccgtg
     2761 gttgcactgg ttgctgcgcg gcgtattttc catatcgcgc agaagggcgt tcatttcggg
     2821 cagggtgagc cggcggccgg cgcggatcga gccgtggcag gacatggtgg cgaggatgtg
     2881 gttttcgtgt tcctcgatgg tttggctgct gccgacttgg gcgaattcgt ttaatacgtc
     2941 gcgggcgagg gagacgacat cggatttgcc caacattgcg ggtgcggcgc ggacggcgag
     3001 ggtgttgccg cccatatcgg acagttccag cccgaagcct gccagtgctt cggcgtggtc
     3061 ggcaagggcg gcgcattctt cgtgggacgc ggcaaaggtt acgggaataa gcaggcgttg
     3121 gctttgcagg ttgccgtttt cctgacgttg gcgtttcatt ttttcgtagt tgacgcgttc
     3181 ggcggcggcg tgcatatcga tgagcaacag gctgtcttcg gcttgggcaa gaatgtagat
     3241 gccaagtaat tgggcaatgg cgaaaccgag cggcggcagt tcggatgggg acgggatgcc
     3301 gtctgaaagc ggcgtatctg tttggggagc aggcgtttca gacggcatat tgccgaaacg
     3361 tgcctgctcg aatcggctta actcaaggtc gatgtcgtcg gtttttttgt aaagttcggc
     3421 gtaagtattc attgccgcgc ggctttcgcg cagggacagg ctgcgttgtt gcggcgcata
     3481 tgcggactga tagggcatgg gcgcggtttt gcctgatgaa ccaaaggcat tgtgtgtatc
     3541 tgatttgttg cctgtcgggt agttggatac gctatcaaac agattttcgc tgtcgttttc
     3601 agacggcatt ggggtggaga caacgccggt aatgtcatgc aacacttcgc ctgcgttgcc
     3661 gacgctttcg gtcaggttgg cgcgtgtgtc ggcaagggct ttgttgagcg tgtggaacac
     3721 gagttggtgc acctgccggc tgtcgcggaa gcggatttct gttttggtcg ggtggacgtt
     3781 gacatctacg gcttcgggcg gcaggtcgag gaaaaggacg aaagcgggtg tcagcgcgtt
     3841 gtgcaatacg tcgcggtatg cctgcttgac ggcgtggagc atcactttgt cgcggacaaa
     3901 acgatggttg acgaagcagt attgtttgtc ggttttgcct ttggcgaaag tcggcttggc
     3961 aatcgcgcca tagagccgca atgcgccatt gccgctgtcg atttccaatg atgccgtctg
     4021 aaaatcatcg ccgacaatgg cggcaatccg ttcatgcagg ctttgtgcag ggagtttgaa
     4081 cacttgtttg ccgtcgcgtt tgagtgagaa ggcaatgtgc ggatgcgcca gtgcgaggcg
     4141 ttcgagcata gtggcgcagt gggcgtattc ggtggcttca gacttgagga acttgcgccg
     4201 cgcgggggtg ttgaagaaga gttcggcagc ttcgatggtg gtgccgacgg ggtgggcggc
     4261 ggcggtgggg ctgctgagtt tgccgtcttc ggctttgact tgggttgcgt gcgaactgtc
     4321 ttcttgacgg ctggtcaggg tcaggcggct gacggaggcg atgcttgcca agccttcgcc
     4381 gcgaaagccc atgctggcga cgtgttccaa gtcatttaag gttttgattt tgctggtggc
     4441 gtggcggtgg agcgcgagtt cgatgtcgtc cggatggata ccgctgccgt tgtcgctgac
     4501 gcgaatcagg cggatgccgc cgccgtccag ctcgacatca atcgccgttg cgcctgcatc
     4561 gatactgttt tcaacgattt ctttcaaggc gttggcaggg cgttcgacta cttcgccggc
     4621 ggcgatttgg ttgacgagat ggtcgggcag ggcggcaatg cgtggcataa ggtaggcttc
     4681 cgttgcaaaa acgctttatt ctataataaa accccttatc tttctgcctg tattcttgat
     4741 aaaagccgtt tatcccgttt ggaaaatacc agtataatca cgccgtatcc cgtaaaattg
     4801 gagcgcaaag atgtatcact accaatccga agccacacaa ttcctcaacc gcctgattga
     4861 agaaaaacct gagttggaac agcagcgttt ggaaaaccgg gggcttttgt gggatgtcga
     4921 actcaatccc gaagaacagg aaaacttcga ggcggcaaaa gtagcgaaaa aaccttatac
     4981 ctattaccaa gactgaaccc gccgaaatga cgacccatct gtccaatgtt gcaccggacc
     5041 tgcaaaacta tttgaacgcc atcggcgaac ccgaacatcc cgttttgacg cggctgcgtg
     5101 agaagaccgg gcatcaccgt atgggcaaaa tggcgattgc gcgcgaacag gcggcggttt
     5161 tggtttggct ggcaaagctg atccgtgcgg aaaaatatct ggaaatcggc gtatttaccg
     5221 gatacagcag caccgcgctt gcattggcgt tgcccgaaca cgggcggatt accgcctgcg
     5281 acatcaatgt aacctttacc gatacggcgc gtcaggtttg gaacgaggcc ggtgtggcac
     5341 ataaaatcag cctgcacctg caacccgcat tgctgacatt ggatgatttg attgcacagg
     5401 gtgaagccgg aagctacgat ttggcactga tagacgcaga caaaccgccc acgccgcaat
     5461 atttcgagcg ttgcctcaag cttgtccggc aaggcggcat cattgccatc gacaatattt
     5521 tgttgaacgg aagggtgatg cgcgaagcgg cttccgatgc gccgcccagc gtcggcatcc
     5581 tcaaagattt taatcaaaac ctgccgaacg atacgcggat tgtccccatc accctgcccg
     5641 tcggcgacgg tttgaccctg cttctgaaaa aataatgaag accaaattac cgctttttat
     5701 catttggctg tccgtatccg ccgcctgttc ttcccctgtt tcccgcaata ttcaggatat
     5761 gcggctcgaa ccgcaggcag aggcaggtag ttcggacgct attccctatc ccgttccgac
     5821 cttgcaagac cgtttggact atctggaagg cagaatcgtc cggctgtcga acgaagtgga
     5881 aaccttaaac ggcaaagtca aagcactgga gcatgcgaaa acacaccctt ccagcagggc
     5941 atacgtccaa aaactcgacg accgcaagtt gaaagagcat tacctcaata ccgaaggcgg
     6001 cagcgcatcc gcacataccg tcgaaaccgc acaaaacctc tacaatcagg cactcaaaca
     6061 ctataaaagc ggcaggttcg ccgctgcagc cgccctgttg aaaggcgcgg acggaggcga
     6121 cggcggcagc atcgcgcaac gcagtatgta cctgttgctg caaagcaggg tgcgtatggg
     6181 gaactgcgaa tccgtcatcg aaatcgggcg gcgttacgcc aaccgtttca aaggcacccc
     6241 tgccgcaccc gaagcaatat tcaaaatcgg cgaatgccaa tacaggttgc agcaaaaaga
     6301 cattgcaagg gcgacttggc gcagcctgat acagacctat cccggcagcc cggcggcaaa
     6361 acgcgccgca gcagccgtac gcaaacgata gtttttccgc tttttacccc atccgcacca
     6421 aggaggattt atgattcaaa tcggcatcat catgggcagc aacagcgatt ggcccgttat
     6481 gcggcaggca gcgcagtttc ttgaagagtt cggcgtagaa tatgaggcgt gcgtcgtttc
     6541 cgcacaccgc accccggatt taatgttcca atacgccgaa accgcacggg cgcgcggcat
     6601 caaagccatc atcgcgggtg cgggcggcgc ggcgcattta cccggtatgg ttgccgccaa
     6661 gaccaccgtc cccgttttgg gcgtacccgt ccccagcaaa tacctgcgcg gcgaagattc
     6721 gcttttatcg attgtacaaa tgcccaaagg cgtacccgtc gccacattcg ccatcggcga
     6781 ggcaggcgcg gcaaatgccg cattgttcgc cgtttccatg ctcgccaacg aaacccccga
     6841 actggcgcaa aaactggcag acttccgagc caaacaggaa caggctgttt tgagtatgga
     6901 attggaacag atttaaaacc cgccgtccgc gaatatatag tggcttaaat ttaaaccagt
     6961 acggcgttac ctcgccttgc cgtactattt gtactgtctg cggcttcgtc gccttgtcct
     7021 gatttttgtt aatccactat acaaatgccg tctgaagccc tgtttcgggt ttcagacggc
     7081 atttttgcga acaacttttt atgcctgttc tttttggcgc attttttctg ccatcagttc
     7141 gtgcagggtt ttcttcgccg gtttcatcgg cacgcggttt tgcgtccaac ccaactgttt
     7201 gcgcggggtc aggttgcgga acttggtggc tgcccaaccg aaggcgcggt aggttttgct
     7261 gccgctgaaa ataccgttga atgtgcgcca cgccatttgt tcgccgaagg tatgcgatgc
     7321 gccttgccct cggatggggt gcggcacggt ttcggtcggc gaacgttgcg cttcaacgcg
     7381 caaacgctgc atttgttcgg taatcgggat gcgtaccgga cagacttcca cacacgcacc
     7441 gcacatggta caggcggttg gcagatcgcg ggtggcatcc agacccaaca ggtgagggga
     7501 aataatctcg ccaatcgggc cgggataggt tgtgccgtat gccgcgccgc cgatgcgggt
     7561 ataaaccggg caatggttca tacacgcgcc gcaacggata cattgcaggg tgcggcgcat
     7621 ttggtcttcg gcataagcct ggctgcggcc gttgtcgagc agaaccaagt gcatttcttg
     7681 cggaccgtct aattcttcac tgcggcgcgg gccggtaatc atattgaaat aagtggtaat
     7741 gttctgacca atggcagaac gcggcagcag gctgtacaag ggtgggatgt cggacaattt
     7801 cgccaccact ttttcaatgc cggtaatagc gatatgcacg ggcggtacgg tggtactcaa
     7861 gcgaccgttg ccttcgtttt ccaccagaca cagcgtacct gtttcagcaa cggcaaagtt
     7921 tacgccactc aaaccgacat cggcagtgct gtaaatatcg cgcagtgctt tacgggcgaa
     7981 gccggtcagt tggtctacat cgtctgtcag cggcgtaccg aggttttggt ggaacagttc
     8041 gctaacctgt tctttggttt tgtggatagc aggcatcacg atatgggtcg gtttttcgcc
     8101 tgccatttgg acgatgaact cgcccaagtc gctttctacc gctttaatgc cttttgcttc
     8161 aagataatgg ttcagctcga tttcctcgct gaccatcgat ttgcctttga ccatcagctt
     8221 gccgtttttg gctgtgatga tgtcgtggat aatttggcag gcttcggtcg gggtttctgc
     8281 ccagtgcact ttcacgccca acttagtcag gttttcttcc agctgctcca gcagggctgg
     8341 caatttagac aatgagcgct gacggacgtg ttcgcacaaa tcgcgcaggc tttgcagctc
     8401 ttcttcgtcg gtcaaaacgg ctttgcgttt ggtcatcagc atatccatcg cggtacgcag
     8461 gcttttgcgc aaaggcttgt cttgaaggga aattgcggcg ttttgcttga aagtttccgg
     8521 cttcatgtga aatttgatgg tttgcgtagt catgcgtttt cctccaaatc ggcaggggaa
     8581 atgtggtcgg gcaggatggc gaggatgacc aaatcgcgcg ggccgtgcgc gccgtaagca
     8641 agcgtcagtt ggatgtctgc ggttttggac gggccggaaa tcaggaatac attggtcggc
     8701 ataccgtttt ccaccagttt ttcgccttcg acggcattat gaaactcgtt gtacatcttg
     8761 gacgtatcga acaggcagaa atgcacgggc ggaacgaggc ttaaagtacg cggctcttcg
     8821 gggctggaaa acagcatcag cgtgccggta cgggcgatgc cgcattgcga gctgctaaag
     8881 cctgcatcga tgttcgcgaa gaactcagtt ttccaagtat cgatttcgcg ctcgaaagca
     8941 atcggttcga tattgctgcc cgccaatgcg gcacgcgcca gttgaccgtg ctcggttgcg
     9001 agaggcagca ggatgttttt caaacccttg ccttctgccg cttcgcggaa aacctgcatc
     9061 caattgcttt tcgtcaccca ataaatttcg gttttgaccg cgcgcatagc ggcagcccaa
     9121 tgtttcagac gctcaacttc gctgccccaa gaaacaccca tttcacggta ataatcaaaa
     9181 accgcaggct cttccatcgg caatgcgtcg gcttttttca gttttgccaa aatattttcg
     9241 cgcgcgctca tgctttgcct ccggtacgtt ccaacaagaa ggatgcgatg tgtttcggac
     9301 gcggcatatt cggttcgtcc ttagcaattt tgccgccgat gttcatcata cagccgcagt
     9361 ccgcgctgat gatttcggtt gcgccggttt ctttcagcgc ggcgactttg tcggttacca
     9421 tcgcgccgga aatatcggct tgtttgacgg agaatgtgcc gccgaagccg caacattcgc
     9481 tttcgtggtc gtggacgatg cgttcgacgt tttccatacc gtcaatcagt tgccagcccg
     9541 agagatggac attcatttcg cggcgggcgg cgcaggaagt gtgaacggcg actttgagcg
     9601 gttcgccctt gtcttcgggt ttgaaaccga tggcaagcag gaaatgggta aactcgatga
     9661 tgcggccggc gcaatccaca gccctttcct cgtactcgct gcctttaaac agcgtcggcc
     9721 aatggtgctt catcataccg ccgcacgaac cggacggtac gacaatcggc cactcctcgg
     9781 ggaaaaggtc gagttgcgct ctggcgacat caaaggcttc ggtcggatgg ccggatgaat
     9841 aggcgggctg accgcagcag ctttgcgcca tcgggaaatg gacgcgtatg ccctgttgct
     9901 cgattagggt aatggcatcc atgcctgctt cgggcataaa aagatcaagg acgcaagtgc
     9961 cgaaaaaata gacatcggtc ggattgctgt cgtatcggat gatttttggg ggaatggttg
    10021 cgctcatgtt ttttgataac gggaaacccg tttttttctg tagaaaggta agcgtttact
    10081 ttaagtaatt gactgttgcg ggtcaagtct aattttaaaa aataatccgg tttttcttac
    10141 aaactgcccc ataacgctta ctgtacctta ctctgataat tttcgataat aattatcatt
    10201 acaataaaat gctgattcgt ttgcttgtga acattcagga cgccgactct gacggcattc
    10261 agacagcatc tgaaaacaat aacggcacaa ggatggggta caatcgccct atggaaaaaa
    10321 acaccctatc cgcccgcgca ccatcccctt ggctgccgct tctgctggca attgccattt
    10381 ttatgcagat gttggatgcg accattttaa ataccgcact gcctgaaatt gccgccgaca
    10441 tgaatgagtc gcctctggat atgcaactgg cagtcgttgc ctatacgctg acggttgccc
    10501 tgctgattcc tttgagcggt tatttggcgg acaggttcgg aacgaaaaaa gtctttttcg
    10561 gttcgattgc cgtttttatg ctcggatcgg cattgtgcgc cgcatcgggt tcgctgtttg
    10621 aattgacgct ttcccgtgtc gttcagggca tcggcggttc gatgctggtt ccgataccgc
    10681 gtctgaccat cttgcgtgtg tacgacaagt ccaagctgct caacgccatc aattatgcgg
    10741 ttatgcccgc attaatcggg ccggttttag ggcctttggc gggcggttat ctggtcgaat
    10801 acgcttcgtg gcactggatt ttcctgctca acctgcccat cggtcttttg ggtttcatat
    10861 tgggacgcaa catcatgccc gatgtcaaag gcaacgatac cgctctggat ttcaaaggct
    10921 atctgatttt ttctgccgcc gcgtgcctct tgttactttc ggcagaaagc ctgtcgcacg
    10981 cgctgcctcc gtattttgca ctgttgccgc tgtgcggcgg actgctgttt gcacgccgtt
    11041 atttccgaca tatgaaaacc gcgtccaaac cgatttattc cgccgacctg tttctgatac
    11101 gcactttccg tctgggactg gcgggcaatc tgttcagccg tctcggcatc agctcgattc
    11161 cttttctgat gcccctgatg tttcaagtcg ctttcggctt cggcgcaagc ctgtcgggtt
    11221 ggctggtcgc ccccgtcgcc ctgtcttcgc tgctggtcaa accgctgatt gcaccgctca
    11281 tgaaacgttt cggctaccgc acggtactgc tttggaacac caagctgctt gccgccttca
    11341 tcatgctgct cgccctgcct gatggaaact cgccgctgtg gatttggatt ttcctctcgc
    11401 tggcgatcgg cgcgtgcaac tccctacagt tttctgccat gaacacactg accctcgccg
    11461 atttgcgccc gcaacagacc ggcagcggca acagcctgat ggcggtcaac caacagcttt
    11521 ccatcagcat gggcattgtt gccggcgcat taatccttaa aaactggaca tttctgatac
    11581 cggcttcttc aggtctgcat tccgccttcc gtatgaccct gctcagtatc ggcggcatta
    11641 cccttgcctc gtcgttggtt ttcaagcgtc tgcacgtttc agacggtgca aacctgacac
    11701 ggaacacacc gtcctaaagc ggtccgcacg caaaactttt acccgtttca acgtttggat
    11761 tatgattccg cacttccatg cgcgccaacc ccaaaacaca ggcaatgccg tctgaaacca
    11821 tatccctgat gaaaacacgc agcctaattt cccttttatg cctccttctc tgttcatgtt
    11881 cttcatggtt gcccccactg gaagaacgga cggaaagccg tcatttcaat acttccaaac
    11941 ccgtccgcct ggacaacatc ctgcaaatcc ggcacacccc tcataccaac gggctatccg
    12001 atatctatct gttgaacgac ccccacgaag cctttgccgc ccgcgccgcc cttatcgaat
    12061 ctgccgaaca cagcctcgat ttgcaatact acatctggcg caacgacatt tccggccgac
    12121 tgctgttcaa cctgatgtac cttgccgcag aacgcggtgt gcgcgtacgc ctgctgttgg
    12181 acgacaacaa cacgcgcgga ttggacgacc tcctgctcgc cctcgacagc catcccaata
    12241 tcgaagtgcg cctgttcaac cccttcgtct tccgcaaatg gcgcgcactc ggctacctga
    12301 ctgacttccc ccgcctcaac cgccgcatgc acaacaaatc ctttaccgcc gacaaccgcg
    12361 ccaccatact cggcggacgc aatatcggcg acgaatactt caaagtcggt gaggacaccg
    12421 ttttcgccga cctggatatc ctcgctaccg gcagcgtcgt cggcgaagta tcgcacgact
    12481 tcgaccgcta ctgggcaagc cattccgccc acaacgccac gcgcatcatc cgcagcggca
    12541 acatcggcaa gggtcttcaa gcactcggat acaacgacga aacgtccaga cacgcgctcc
    12601 tgcgctaccg cgaaaccgtc gaacagtcgc ccctctacca aaaaatacag gcaggacgca
    12661 tcgactggca gagcgtccaa acccgcctca tcagcgacga ccctgcaaaa ggactcgacc
    12721 gcgaccgccg caaaccgccg attgccgggc ggctgcaaga cgcgctcaaa cagcccgaaa
    12781 aaagcgtcta tctggtttca ccctatttcg tccccacaaa atccggcaca gacgcactgg
    12841 caaaactggt gcaggacggc atagacgtta ccgttctgac caactcgcta caggcgaccg
    12901 acgttgccgc cgtccattcc ggctatgtca aataccgaaa accgctgctc aaagccggca
    12961 tcaaactcta cgagctgcaa cccaaccatg ccgtccctgc cacaaaagac aaaggcctga
    13021 ccggcagctc cgtaaccagc ctgcacgcca aaaccttcat tgtggacggc aaacgcatct
    13081 tcatcggttc gttcaacctc gacccccgtt ccgcgcgtct caacaccgaa atgggcgttg
    13141 ttatcgaaag ccccaaaatc gcagaacaga tggagcgcac ccttgccgat accacccccg
    13201 aatacgccta ccgcgttacc ctcgacaggc acaaccgcct gcaatggcac gatcccgcca
    13261 cccgaaaaac ctacccgaac gaacccgaag ccaaactttg gaaacgcatc gccgcaaaaa
    13321 tcctatccct gctgcccata gaaggtttat tatagaaatg ccgtctgaaa caccttcaga
    13381 cggatatccg aacccgcaaa ggaaaaacca tgtttccccc cgacaaaccc cttttcctct
    13441 gtctcagcgc actactcctc gcctcatgcg gcacgacctc cggcaaacac cgtcaaccga
    13501 aacccaaaca gacagtccgg caaatccaag ccgtccgcat cagccacatc gaccgcacac
    13561 aaggttcgca ggaactcatg ctccacagcc tcggactcat cggcacgccc tacaaatggg
    13621 gcggcagcag caccgcaacc ggcttcgatt gcagcggcat gattcaattc gtttacaaaa
    13681 acgccctcaa cgtcaagctg ccgcgcaccg cccgcgacat ggcggcggca agccgcaaaa
    13741 ttcccgacag ccgcctcaag gccggcgacc tcgtattctt caacaccggc ggcgcacacc
    13801 gctactcaca cgtcggactc tacatcggca acggcgaatt catccatgcc cccagcagcg
    13861 gcaaaaccat caaaaccgaa aaactctcca caccgtttta cgccaaaaac tacctcggcg
    13921 cacatacttt ttttacagaa tgagccgccc gccgcgccgc gccggaatcg cggcaaaatg
    13981 ccgccttatt ccgctgtttc ggtcagcgat gagaacacgt cgaaataagt cgggaaggtt
    14041 ttgtgggtgc atttcggatc gttgatgacg acgggtacgc ccaacagcga aaccagcgag
    14101 aaacacatcg ccatgcggtg gtcgtcgtac gtgtcgatga cggcgtcggg tgtcggcgtt
    14161 tcgggcgggg tgatgtgaat tgcttcggct tcttcgacga cttttgcccc gagtttgcgc
    14221 aactcgtttg ccatcgcggc gatgcggtcg gtttctttga cgcgccacga accgatgttg
    14281 cgcagcgtgc aggtttgccc tgtagcaagc gcgacgatgg cgagggtcat ggcggcatcg
    14341 gggatatggt tcgcatccaa atcaaaggat tggacggcac gttccttcgg gcgtgaaact
    14401 tcgacgaagt tttcgcccca aaccacgtcc gccccgattt tttccagctc gcgggcaaag
    14461 gcgacatcgc cctgtatgct gtttgcgccg ataccgataa cacagacggg cgtggcggca
    14521 atcaaaccgg ctgcgaggaa gtaggacgcg ctggaggcat cgccttcgac gtgcaagtgt
    14581 tcgggcgcgt ggtagtgcgc atcggcggga attttgaaga cgcggtagtt ttcattggca
    14641 acctgtacgc cgaattgcgc catcagtttt aaagtaatgt cgatataggg cttggaaatc
    14701 aattcgccga ccatacggat ttcaaacgcc tgcccggtca gcggcaacgc cattaaaagg
    14761 gcggtcagaa actggctgga cacattgcct ttaatcggaa tcacgcgttc gccgttgtct
    14821 tgacgtttgc cgatatgaag cggcggatag tgttccttgc cgagatattc gacatcggcg
    14881 ccggcaatcc gcagcgcatc ggccaaatcg ccgatggggc gttcgtgcat acgaggcacg
    14941 ccgtgcagat gataatcgcc gcccaaaacg gcaagtgcgg cagtcagcgg acggaacgcc
    15001 gtgcccgcgt tgcccaaaaa caaatcggca gtgcggttgg ggaagcgtcc gcctgtgccg
    15061 tgcactttca gacggtcttc ggcaagatat tcgatttcaa cgccgagttt atcgagtgct
    15121 tcgagcatac ggtcggtatc gtcggatttg agcagggaat ggatttcaca agtattgtcg
    15181 gacaaggcgg cgagcagcag ggtgcggttg ctgatgcttt tggagccggg aagggcgacg
    15241 gtggaaggtt tgagcgaggc ggcagggagg cggagggatt cggtcatggc aaaacgtcaa
    15301 tattataaag ataaaaacag cctgcattat actggtgcaa atgctgtatg aaaaatctca
    15361 ggtttggcat tttcggtttt aaagtccgtg aatgtggttt tttatgccga aaattgatta
    15421 tttttaaatt ttttgtttct aaaatttttt tgccggcata ttttcggctt ttgttgcggg
    15481 caggtggcgt ttgcggacgg tacggtacga ttgccaattt catagaattt ggtagaatag
    15541 ccgctgttca acgacagaca agccgccgat tttccgggcg gcttgtattt ttattttaat
    15601 atccggacgc acaaaacaag acggtacgcg gtatgcttgc tgccgcaaaa cggtgtgccg
    15661 gaatatggag aaaaaagacc gatgcaaaaa atccccctga ccgtacgcgg tgcggaattg
    15721 ctgaaacagg aattgcagca gctcaaaagc gtggcgcgtc ccgaagtgat cgaagcgatt
    15781 gccgaagccc gttcgcacgg cgatttgtcc gaaaacgccg aatacgaagc cgccaaagaa
    15841 cgccaaggct ttatcgaggg ccgcatttcc gagctggaac acaaactttc cgttgcccac
    15901 atcatcaatc cgaccgaaat ccacgccgaa ggcaaaatcg tgttcggtac gacggttacg
    15961 ctggaagatt tggaaacgga agaacacgtt acctaccaaa ttgtcggcga agacgaagcc
    16021 gacatcaaac agggcaaaat ctatgtcggc tcacccatcg cccgcgcctt aatcggcaag
    16081 gaagaggggg atacggcgga agtccaagcc ccgggcggcg tgcgcgaata cgacattatc
    16141 gaagtccgat atatttgatt cggcttgatt tcgatacacc cgacacacgc aggaaattat
    16201 agtggattaa caaaaatcag gacaaggcga cgaagccg
//
LOCUS       DAXHKX010000027        14875 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHKX010000027 DAXHKX010000000
VERSION     DAXHKX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.027"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(93..3578)
                     /gene="smc"
                     /locus_tag="ACFMWZ_000722"
     CDS             complement(93..3578)
                     /gene="smc"
                     /locus_tag="ACFMWZ_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC5934717.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETVERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTRQDEAN
                     RIRRELALKQQQLAHTGQTIAGHEERKGRLKQENQALNLPDEAETAAAQEAAALLQGR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQETAD
                     FWQATGHPAAPQLWQHITAPAEWQHALSVILAERLHARSVPSGFVPPEPLPQGQAAWL
                     SDELSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPELSAAEAAFKQAE
                     AAVRSSETQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTFDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNSKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3827..4549
                     /gene="lpxH"
                     /locus_tag="ACFMWZ_000723"
     CDS             3827..4549
                     /gene="lpxH"
                     /locus_tag="ACFMWZ_000723"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC5934718.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            4818..6404
                     /locus_tag="ACFMWZ_000724"
     CDS             4818..6404
                     /locus_tag="ACFMWZ_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC5934719.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6573..6776)
                     /locus_tag="ACFMWZ_000725"
     CDS             complement(6573..6776)
                     /locus_tag="ACFMWZ_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC5934720.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6892..7389
                     /locus_tag="ACFMWZ_000726"
     CDS             6892..7389
                     /locus_tag="ACFMWZ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC5934721.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7471..8664
                     /locus_tag="ACFMWZ_000727"
     CDS             7471..8664
                     /locus_tag="ACFMWZ_000727"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC5934722.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVKVL"
     gene            complement(9068..10003)
                     /gene="hemC"
                     /locus_tag="ACFMWZ_000728"
     CDS             complement(9068..10003)
                     /gene="hemC"
                     /locus_tag="ACFMWZ_000728"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC5934723.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10122..10637
                     /gene="ybeY"
                     /locus_tag="ACFMWZ_000729"
     CDS             10122..10637
                     /gene="ybeY"
                     /locus_tag="ACFMWZ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC5934724.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQEDGH"
     gene            10639..11463
                     /locus_tag="ACFMWZ_000730"
     CDS             10639..11463
                     /locus_tag="ACFMWZ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC5934725.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11592..12971)
                     /gene="nhaC"
                     /locus_tag="ACFMWZ_000731"
     CDS             complement(11592..12971)
                     /gene="nhaC"
                     /locus_tag="ACFMWZ_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC5934726.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13261..14484)
                     /locus_tag="ACFMWZ_000732"
     CDS             complement(13261..14484)
                     /locus_tag="ACFMWZ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC5934727.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3527 a         4207 c         3810 g         3331 t
ORIGIN      
        1 taccgattta aatttaattc actataccgt ctgaaaaccc gtatcgcaac acgtcattaa
       61 aaacggcaag attcagccgt tctgcaaccc gttcaaacgg cttccgccat ttccaacgcc
      121 tgtttgatgt ccacggcgac gacgcgcgac acgccttttt cctgcatggt tacaccgacg
      181 agctgctccg ccatttccat ggtcaggcgg ttgtgggaga tgtagagaaa ctgggtttgc
      241 gccgacattt ctttgaccag gttgcagaaa cgcgaagtgt tggcatcgtc cagcggggca
      301 tcgacttcgt ccaaaaggca gaagggggcg gggttgaggc tgaacagggc gaacacgagg
      361 ctcatggcgg tgagggcttt ttcgccgccg gagaggaggt ggatggtgct gtttttcttg
      421 ccgggcggac gcgccatgat ggacacaccg gcggtcagta ggtcgtcgcc tatcattttg
      481 agagtggctt cgccgccgcc gaacagggtc gggaagaagg tttggacttt gctgttgacg
      541 gcatcgaagg tttctttgaa acgcgctttg gttttgtcgt cgatttgggc gatggcttct
      601 tccaaaaggg tgatggctgc ctgcacgtct tcgctttggc tgcggtagta gccgtcgcgt
      661 tcgcgcgctt cttcgagttc ttgcagggcg gcgaggttga ccgcgccgag tgcttcgatt
      721 tgttgcgaaa ggctgccgat gctgctgttc aatactttcg gcgattcttt cgccaacgct
      781 tcgagcgcgt ccaaatcggc ggcgcgttcg gtcaggtttt gatggtagcg tttggcgtta
      841 atcagggctt cctgctgctg caacagggcg gtttgggtgg ctgcctgaag ctgtggcaac
      901 tgggtttgca gggtttgtac tttagcgtat tgttccctgc cctgttcctg aatctgcgcg
      961 agtttctctt gcacaacaat atattcttcg tccaaggtct gtacggcttc gcttaattct
     1021 tcaagcttga tgtgctgctc gtcgttttgg aactcggttt cataggcgag ggcaagctct
     1081 tgttggcgtt cctgccaatc aaaggtttgc tgttcgagct gggcaatttg ctgccggtag
     1141 ttttgttttt gctggttgag cttgtgaacg gcgacttcgg ccagcccgta ttggcggttg
     1201 gcttccaaca gggcaagctg cgcctgtttc agacggcctt gctgctcttg gcggctgtgc
     1261 gcggtggttt gctgctggtg ttcgagttcg gcggcggctt cctgcaaggt aacgatgtcg
     1321 tctgaaagcc cgtcggacgt gtgttgcaac acggtctgct cttccgccaa ctgcgccagt
     1381 tcgcgctcga tgtgttcgcg gcggatttgc ccttggttgg tgcgcgccaa gagttcggcg
     1441 gcgcgttgct gcgcttggct gtattggcgc gtgtgctgct gttgctgctg catcaggttt
     1501 ttgtgttgcg tctcagacga gcgcacggcg gcttccgcct gtttgaacgc ggcttcggcg
     1561 gcggaaagtt cgggggcgag gttttccagt tcggacgcga tgccgtcgag gcgtgctttt
     1621 tgggcaatca ggctttcctg cgcgggttta gcatagagca ggacgctgac tttgtcgacc
     1681 tgatggcctt cgggcgtgag ccagatttga tgcgcgccca aatcgctttg atgcgcgaga
     1741 gcgtagctca aatcgggtgc gcacaatacg ccgtcgagcc agtggtgcaa tgccgtctga
     1801 aacggcggct gcgcttggat ttggttcaac aatgcctgta cgggtaggga ttttttgatg
     1861 ccgccggaga gctcgtctga aagccatgcc gcctgccctt ggggtaaagg ttcgggcggc
     1921 acgaagcctg acggcacgct acgggcgtgc aggcgttcgg caaggatgac ggacagggcg
     1981 tgttgccact cggcgggcgc ggtgatgtgt tgccacagtt gcggcgctgc ggggtggccg
     2041 gttgcctgcc agaagtcggc ggtctcctgc tgttgcgaca ggatttgcga caacgcctgc
     2101 tgctgcgcct gcaaggtgat gtgttgctgc ttcaggcttt ggaagcggtt tgatgccgtc
     2161 tgaaacgcct cgcgggcggc gtgcaaggct tcttcggcgg cgatgatttg ttcttcgtaa
     2221 tgctcttgcc ggccttgcaa caaggcggcg gcttcctgcg cggcggcggt ttcggcctcg
     2281 tcgggcaggt tcagggcttg gttttcctgt ttcagacggc ctttgcgctc ttcgtgcccg
     2341 gcaatcgttt gtccggtatg ggcaagctgc tgctgcttca acgccagttc acggcggatg
     2401 cggtttgcct catcctgccg ggtttggaag gcggcgttga gcgtggcttg ggcttcttcc
     2461 aattcgggca gacgctcttc gtgttcggca acctgcatcg cccactccgc cagctcggtt
     2521 tgcttttctt cgacgtataa ctcgttttct tcaagctgca cgcggatttg ctgctgctct
     2581 tgatgaatac gctgcaactg cgcctgtgcc gcctgcttgt cgcgttcgat gcgttgatgc
     2641 aggttttgcc ggtggcggat ttgttcttcc aaacgggcaa tctgctcgcg caacacgccg
     2701 cgtttgttgc ccaattcgtg taccgcctgc tgctgcgact gttcggcggt ctgcaaggca
     2761 tgtacttcgt cgtttaacgc ctgaacctgc gcggcggttt cgtcctgctg cgcctgcaaa
     2821 gactgatgct gcgcggtcgc cttgtcggcg gcggcaagcg attgctgcca ttgggcgtaa
     2881 tcgagcaaat cctgctgttg gttcaactgc gcggtcaggg atttgtagcg ttcgacggtt
     2941 tccgcctgtt tttccagctt ttccacctga cgcgccaact cgttctgcaa atcacccaaa
     3001 cgctgcaaat gctcgcgcgt gtctttcaga cggccttccg tctccttgcg gcgttcctta
     3061 tatttggaca cgcccgccgc ctcctcgata taggcgcgca actcctccgg ccgcgcttcg
     3121 atgatgcgcg aaatcatccc ctgctcgata acggcataac cgcgcgcgcc cacgccagta
     3181 cccaaaaaca aatcggtaat gtcgcggcgg cgcacggtct gattattgat gaaataagtc
     3241 gattcgccct gacgcgtcaa ctgccgcttg atgctcacct cggcatactg cccccacgcc
     3301 ccctgcaaac tgtggtcgct gttgtcaaac accagctcca ccgaagccct cggcgcagga
     3361 cggcgcgtcg ccgcaccgtt aaaaatcacg tcctgcatac tctcgccgcg aagctgcttc
     3421 gccgaagcct cacccaacac ccagcgcacc gcgtcaatca cattcgactt gccgcagccg
     3481 ttgggcccga taaccgcgac aagctgcccc ggaacatgaa tcgtggtcgg gtcggtaaaa
     3541 gatttgaagc cggagagttt gatgtgggtc aggcgcataa tggtcggaaa aaataaaaaa
     3601 gaagcgtatt ttaacggaaa tcccgccgca ccaccccata tcttgccggc aaaaccttac
     3661 cgcatcctac gcccgcgatg ccgtctgaag ccttcagacg gcatttttaa cgcgcccgaa
     3721 ccccggttcc actaacgccc gatgcttggc gcggcatttg ccgtattgcc ccgccgccgg
     3781 gcgcgttata atccgccacg acatccgccc gattggaaac ctgcctatga aacccgccta
     3841 tttcatctcc gacctgcatt tgagcgaaaa gcaccccgaa ctgaccgcgc tgctgctgcg
     3901 ttttttacgt tcttccgccg ccgggcaggc gcgggcgatt tatattttgg gcgatttgtt
     3961 tgatttttgg gtgggcgatg acgaagtttc cgagttgaat acttcggttg cgcgggaaat
     4021 caggaaattg tccgacaaag gcgttgccgt gttcttcgtc aggggcaacc gcgacttcct
     4081 gatcggtcag gatttttgcc ggcaggcggg tatgacgctg ctgccggatt actcggtttt
     4141 ggacttgttc ggctgcaaaa ccctcatctg ccacggagac acattatgca ccgacgacaa
     4201 ggcataccaa cgtttccgca aaatcgtgca tcggaagcgg ctgcaaaaac tgttcctgat
     4261 gctgcccctg aagtggcgca cgcgccttgc cgccaaaatc aggcgtgtca gcaaaatgga
     4321 aaaacaggtc aagcccgccg atattatgga tgtcaatgcc gcctttaccg cgcggcaggt
     4381 acgcgccttc ggtgcggaaa ggctgataca cggacacacc caccgcgagc atatccatca
     4441 cgaaaacggc tttacccgca tcgttttggg cgactggcat aacgactatg cttcaatcct
     4501 ccgcgtggac ggggacggcg cggtattcgt gccgctggaa aaatactgaa aatgccgtct
     4561 gaagcctttc gggcggcttt ttttacacgc ccctttccca cactccctcc gccgcatcat
     4621 caccatcctt aacccgcatt aatagccatt atcgataaaa tgcttgaccg attacataag
     4681 attacgtaaa gtgtgcaaac gcataacaat tggtcttacc aatttatctt ttaaccaaaa
     4741 tatcgggtgt acgggatttg aacgtctttc caaacctcga cgcgccttat gatgcaaatg
     4801 cctaaaaagg attcgttatg gcacttttcc tcagcatatt ccccatcgtc ctgctgattt
     4861 ggctgatggt gaaaaaaaac agtatgccct cctacgtcgc gctgccgatt accgcagtgc
     4921 tgatttacgc catcaaactt ttctacttcg acgatgcggg catgctgctc aacgccaccg
     4981 ccgcttccgg cctcgtcaaa acgctcacgc cgattaccgt gattttcggc gcgattatgt
     5041 tcaaccgtat gatggaaacc acgggctgca tcgatgtcat ccgcaaatgg ctggcgacca
     5101 tcagcccgaa ccccgtagcg caactgatga ttatcggctg ggcttttgcc tttatgattg
     5161 aaggcgcatc cggcttcggc acgcctgccg cgattgccgc gccgattctg atgagcttgg
     5221 gcttcaaccc gttgaaagtg gcgattttca ctttggtgat gaactccgtc cccgtctctt
     5281 tcggcgcggt cggtacgccg acctggttcg gtttcgcacc gctgaacctg agtgccgaag
     5341 acatcctcgc catcggcagg cagaccggcg taatgcactt cttcgcaggt ttcgtcatcc
     5401 ccgtcatcgg cttgggcttc atcgtacctt ggtctgaaat ccgcaaaaac ttgggcttcg
     5461 tcgccattgc cgtcttctcc tgcaccattc cttatgtcgc attggcgatg gtcaacgaag
     5521 aattcccgtc gctcgtcgcc ggcgcgatcg gcctgatggt gtccgtattc gccgccaacc
     5581 aaggctgggg cttgagcaaa gaccacgcca aagacccgaa tgccgaaaaa gtgccgttcg
     5641 cccaagtcgc caaagcactc gcccctttgg gtatgctgat cggcatgctg gtggttacgc
     5701 gcatcaaaca gctcggcatc aaaggcattt tgaccagcaa agaagaatgg ttcagcttcc
     5761 aactgccgtt tgatttgtcc aaaatcaccg tcagcgactc cctgacgatt actttcggca
     5821 atattttcgg acaagatgtc agcgcgtctt accaaacgct gtacgtcccg gcttggattc
     5881 cgtttgtgct gaccgtttgg atttgcatcc tgctgtataa aaccaaattc aaagatgcct
     5941 ggacgattta tgccgtaact ttcaatcaaa ccaaaaaacc gctgcttgcc ctgatgggcg
     6001 cgctgattat ggttcagctg atgctggtcg gcggcgacaa ttcgatggtg aaaatcatcg
     6061 gtaaggaatt tgccgcaatg gcgggcgaac actgggttta tttctcgccg tatctgggcg
     6121 cgatcggtgc gttcttctcc ggctccaaca ccgtgtccaa cctgaccttc ggaccgattc
     6181 agcagcaaat cgccttggat accggcctgt ccgttaccct gattctggcg ttgcagtccg
     6241 tcggcggcgc gatgggcaat atggtgtgcc tcaacaacat catcgccgta tgtaccgtat
     6301 tggacgtgaa aaattccgaa ggtgcgatta tcaagaaaac cgttatcccg atggcgattt
     6361 acggcgtgat tgccgtcgtc gcggcaatga ttttcttcct ctaggcgaaa cgccaccatc
     6421 catagcggca gcacgcgggg aacggaaacc gccgttcccc gtccgggcgg cgcgtcgggc
     6481 agaatcccgg tgccggaaca agcccgcccc gccaaacaaa tgccgtctga aaccggaaaa
     6541 ggcttcagac ggcatttttc cgccgcttgc gttcaggcgt aatgatgatg cggcatcttg
     6601 ccgtcaaggc ggtagcgcgt gccgcaatac gggcaggcaa cgctgcccga ttctccttcg
     6661 cacaaaggca aaaacactct cggatgcccg ttccactgct cgttgtcggg tcccgagcaa
     6721 tacagcggca gattttccgg caacacggaa atttcctgca gattcagatt gtccatttga
     6781 ttttcctttg cgtggttggc gtgcgcccat tttacgccat cggcaggcta aaggatattt
     6841 tcggcgcaaa gccgcaatcc gctataatcc ccacttttca gacggcatac catgactgcg
     6901 cttacccttc cggaagacat ccgccaacaa gagccatccg ccctgctcta taccctcgtt
     6961 tccgcctacc tcgaacacac cgcccaaacc ggcgacgaat ccctctcctg cctgtccgac
     7021 gaccagcaca cgctgaccgc attctgctac ctcgacagcc aagtcgaaga aggcggcttc
     7081 gtgcaactca tcgcctccgg ttacggcgaa tatattttcc gcaacccgct tgccgacagc
     7141 ctgcgccgct ggaaaatcaa agccgtgccg aaagtcttgg acaaagccaa agccctctac
     7201 gaacaacacg gcaaaaccat cgaaacgctc gccgacggag gcgcagacat cgaatccctg
     7261 cgcaaacagt tccccgactt tgaagaatgg gacggcgcat actacgaagc cgccgaacaa
     7321 gacctgcccc tgcttgcaga acacatacag tcaaactggg aaaccttcgc ccatatcggg
     7381 caggcgtgat tgcgtctgtt tccagcccgt gtaaaacagc gtaaaatcgg caaacccgaa
     7441 tgaccttccg tccccatcaa ggagcaagct atgttcttca agcacatcga agccgccccc
     7501 gccgatccga ttctcggttt gggcgaagcg ttcaaagccg aaacccgccc cgaaaaagtc
     7561 aacctcggca tcggcgttta taaagacgca tccggcgcga cacccctcgt caaagccgtc
     7621 aaagaagccg aaaaacgcct gttggaaagc gaaaccacca aaaactacct caccatcgac
     7681 ggtgttgccg actacaacgc gcaaacccaa atcctgctgt tcggcaaaga ccacgaaatc
     7741 atcgccagcc gtcgcgccaa aacagcgcaa agcctcggcg gtacgggcgc attgcgtatt
     7801 gcggccgagt ttgccaaacg ccagttgaac gcgcaaacca tctggatttc caatccgact
     7861 tggcccaacc acaacgccat cgccaaagcg gtcggtatcc aagacaaacc ttatcgttac
     7921 tatgatgccg ccaaacacgg tttggattgg gacggcatga ttgaggactt gagccaagcg
     7981 caaaaaggcg acatcgtcct gctgcacggc tgctgccaca atcctaccgg catcgaccct
     8041 acgcccgaac aatgggaaac tttggcaaaa ctttctgccg aaaaaggctg gttgccgctg
     8101 tttgactttg cctaccaagg cttcggcaat ggtttggaag aagatgccta cggcttgcgc
     8161 gtgttcttga aacacaatac agaattgctg attgccagct cttattccaa aaacttcggt
     8221 atgtacaacg agcgcgtcgg cgcgttcact ttggtggccg aagatgaaga aacagcagcc
     8281 cgcgcccaca gccaagtcaa aaccatcatc cgtaccttgt attccaaccc ggcttcacac
     8341 ggtgcgaaca ccattgcgct ggtgttgaaa aatgatgatt tgaaagcaca atggattgcc
     8401 gaactcgatg aaatgcgcgg ccgcatcaaa gccatgcgcc aaaaatttgt cgggttgctc
     8461 aaagccaaag gtgcaagcca aaactttgat ttcattatca aacaaaacgg tatgttctct
     8521 ttcagcggct tgactcccga acaagtcgac cgcctgaaaa acgagtttgc catttatgcc
     8581 gtccgctccg gccgcatcaa cgtcgccggc attaccgaca acaacatcga ttatctgtgc
     8641 gaaagcatcg tgaaagtact gtaaaaagca cagccatatg aaaatgccgt ccgaacctta
     8701 accagttcag acggcatctg ttttttgtta aaaaacaata aaccataaca atttcaaaca
     8761 agctaaaaac atctataaaa tcaaccaaat atgatgaact caaataaaaa atacgaatat
     8821 catcattctc aaattcaaaa tttgaacgcc acagtattat ccgaagccac aaaaaccttt
     8881 ccgccgtcat tcccgcgcag gcgggaatcc gggaatccaa tgctgcaaga attgattaga
     8941 aacaactgaa accgagcaaa ctggattccc gcctgcgcgg gaatgacgaa ataaggcgag
     9001 tgaaactgat gaaacaaatt gattaaaaac aataacttat attattttat ttaaattttc
     9061 gataaaatca attttccgta ttcaatactg ctccaatcaa ttcccgcgca ccgtcgtccg
     9121 ccaatttctt agcgacagcg cgtccgagcg cgtcggcata ttcggcaggg gcttgcgcgt
     9181 ccgcccgcaa cacaaccgaa ccgtcggggt gtccgaccaa gccgcgcaag gtcagcaagc
     9241 cgttttcttc cgtgcaatat gccgccagcg gcacttggca gcttccgccc aaagcgcgtg
     9301 cgagggcgcg ttcggcggta acgcaggcat tggtaacacc gtggttcaag ggtttcaaaa
     9361 cttcatacaa atcttcgcgg tgcgcggcaa tttcgatacc caatgcgcct tgtccggcgg
     9421 caggcaggct gtcggattcc gacaaaatca tgcggatgcg tccgtccaat ttcagacgct
     9481 gcaaaccggc ggcagccaag ataattgcgt cgtattcgcc gttatcgagt ttggacaaac
     9541 gggtttgcac attgccgcgc aaaggtttga taagcaaatg cggatagcgc gcacgcaact
     9601 gggcttcgcg gcgcaggctg gatgtgccga caaccgcgcc ttcgggcatt tcttccaaac
     9661 gcgtgtattg gttggacaca aacgcgtcaa acggattggc gcgttcgccg atggcggcaa
     9721 gcgcgaaacc ttcaggcaaa tccatcggca cgtccttaat cgaatgcacc gccaaatcgg
     9781 cgcgcccgtc atataaagcc tgttccaact ctttgacaaa caagccttta ccgccgactt
     9841 ttgacaaagt tttgtccaaa atctgatcgc cgcgcgtggt catgcccaaa atctcgactt
     9901 cgcaatcggg atacagagcc ttcagacggc cttggatatg ctttgcctgc cacatagcaa
     9961 gcaggctttc gcggctggcg atgacgagtt ttttcgggtt catgttcaat gcgttcaaag
    10021 aatggaaacg gcgtgaagtc tatcatattt tgaacgtttc ttcagacgac accgtcaggt
    10081 ctatgttata tttacaccca caaaacacga aacccgccac aatgaaacgc accaaaaaat
    10141 accctttctt aacgttgcag cggcaacgtt tccatttgaa ctttgaaaac gcctcttccg
    10201 ccgccggcat cccagccgaa cgcgatttct accgctgggc gtggtctgcc ttgaaaaatg
    10261 aataccgccg cgccgacatc agcctgattc ttctggacga agaagaagcc cgagcctaca
    10321 accgcgacta ccgcggcaaa gattacgcca ccaatgtatt gagtttcgcg ctcaacgaag
    10381 gcgaaatcct gccctgccaa gtttcggaaa aactgtacgg cgatttgatt atctgcccgc
    10441 aagtggtttt gaaagaagcc gccgaacaag gcaaaacacc cgagcagcat tttgcccacc
    10501 tgaccataca cggtacttta cacctgatgg gctacgacca catcgaagac gacgaagccg
    10561 aaataatgga agccgaagaa atccgcctga tgcgggcggc aggcttcccc aacccctacc
    10621 aagaggacgg acattaaaat ggacggcgca caaccgaaaa cgaatttttt tgaacgcctg
    10681 attgcccgac tcgcccgcga acccgattcc gccgaagacg tattaaacct gcttcggcag
    10741 gcgcacgagc aggaagtttt tgatgcggat acgcttttaa gattggaaaa agtcctcgat
    10801 ttttccgatt tggaagtgcg cgacgcgatg attacgcgca gccgtatgaa cgttttaaaa
    10861 gaaaacgaca gcatcgagcg catcaccgcc tacgttatcg ataccgccca ttcgcgcttc
    10921 cccgtcatcg gcgaagacaa agacgaagtt ttgggcattt tgcacgccaa agacctgctc
    10981 aaatatatgt ttaaccccga gcagttccac ctcaaatcca ttctccgccc cgccgtcttc
    11041 gtccccgaag gcaaatcgct gaccgccctt ttaaaagagt tccgcgaaca gcgcaaccat
    11101 atggcgattg tcatcgacga atacggcggc acatccggct tggtcacctt tgaagacatc
    11161 atcgagcaaa tcgtcggcga aatcgaagac gagtttgacg aagacgatag cgccgacaat
    11221 atccatgccg tttcttccga acgctggcgc atccatgcag ctaccgaaat cgaagacatc
    11281 aacaccttct tcggcacgga atacagcagc gaagaagccg acaccattgg cggcctggtc
    11341 attcaagagt tgggacatct gcccgtacgc ggcgaaaaag tccttatcgg cggtttgcag
    11401 ttcaccgtcg cacgcgccga caaccgccgc ctgcatacgc tgatggcgac ccgcgtgaag
    11461 taagcaccgc cgtttctgca cagtttagga tgacggtacg ggcgttttct gtttcaatcc
    11521 gccccatccg ccaaacataa aaatgccgtc tgaaaccgtt gcaagtttca gacggcattt
    11581 cgcttatccg cttatttttt gctcaaagtc agccccgtcc aaccgaataa cagggtcaaa
    11641 gccaaactca aatagcagaa aaaggcatac ggcagatatt cccaaaccgg cacgcccagc
    11701 gcgtggctga tgaacacgcc gcatacgctc cacggtacga gcgggttaat caccgtcccc
    11761 gcatcttcca gcgtccgcga cagattgcgg gaatgtagtc cgagcctgtc gtaaacgggt
    11821 ttaaacgttt ctccagaaag caggatgctc aaatattgtt cgccaatcag gaaattaacc
    11881 ccgaccgaag tcatggcaac gctgaacgtc gcgcgtccgg cattcgtcaa gaaggtacgg
    11941 atggcctcca acagggaagg aatcacaccg agcgcaaaca acagtccgcc caaactcatc
    12001 ccgagaatca cgatggtttg cgtgaaaaac atactttcca aaccgccgcg cgaaatcagt
    12061 ttgacaacat ctttaaacgc ttcgccttcg agtttgtagc cgccgtaaaa ccacgcaccg
    12121 agctgacgca gatcgggcgt gctgtgcaga tacgttacag caacggcaac catgacggta
    12181 aagagcatgg cgacgacggc gttgatgcgc atcaatgcca aaatgaccaa cagcgcaaac
    12241 ggaatcagcg aatagccgtg caccaatccc gtggcttcaa gctggctgcg gaaggattcg
    12301 acgctgttca aatcctgcgc ggcgacattc ggcaaaagcc aaagcatcag tgccgcacta
    12361 atgagccacg cggggatggt ggtgtacatc atatttttga tgtgctcaaa caagtcgatg
    12421 ccgacgatgg acgcggaaat acccgtcgta tccgaaagcg gggacatttt gtcgccaaaa
    12481 aatgcgcccg aaacaatcgc gcccgccgtc atcgccatat cggcctgaaa cgccgccgcc
    12541 atccccataa aggcaacgcc gacagtggcg caggtggtca ggctgctgcc gatggacacg
    12601 ccgatgacgg aacacagcgc gaaggcggaa aaataaaaat aagtcgggga aatcagtccg
    12661 aaaccgtaat acatcagcgt cggaatcgcg ccgctcatca tcagcgcgct gaccatcagc
    12721 ccgatgaaga aaaacaggta aatcgcgccc ataccctgat tcaacgcgcc tatcatgccc
    12781 tgctgcatat cgttgtattt caaaccgcgc gccaagccgt acaaaatcag cacgacgatg
    12841 gcggcaataa tggacatatg cggcagccac tccaatgaaa tgatggtata gcccatcgcg
    12901 gcaatcagag cgacgacgac ggcaagtgcc tcaccgcgcg gcatatcgag taaggatttg
    12961 aaagcgaaca tgattctttg cccgatttga tataaagtta acggattcta acacaccata
    13021 ccgaatccgc ccgacgtttt cagacggcat tttagcgttt tacaaaagcc ggacagaaac
    13081 aaaacggcaa ttttctcaat ttaaattaat ttttttgaaa tataattttt tattttttgt
    13141 ttgttggcat ataagacttt tatggattga agtattaaaa cctcggcaca cacgccgcaa
    13201 acaccggtac gcaaaaatgc cgtctgaaag atgttcagac gacatttctc aagaaaaaga
    13261 ttatttgtcc gccctatatc cgtacaccgc aaaaaacacg atatacagat aacagatgac
    13321 ggaaacgaca aacgaagaca tcagggtgta agtatctgcc acccagccct gcacgacagg
    13381 aacgaccgca ccgccgacaa tcgcggtaca cagtacaccg gaagccgcgt tggtaaattt
    13441 tcccaatcct ttggttgcca aagagaaaat cgtcgggaac ataatcgagt tgaaaaaacc
    13501 gatggcaagc agcgaccaca tcgccacatc cgcattgccg ctacccgtcg ccatcgcgac
    13561 ggcaagcagt acgaccgcag ccgatgcgtt aaacgccaaa taacggttgg gcgcgaattt
    13621 cgccatcacc gccgaaccga ggaaacgtcc gaccatcgcg ccgccccaat agaacgacag
    13681 gtaatgcgcg gcagaagcat gatccagccc tttcagataa cccaatacgt tgaccatcaa
    13741 cgaaccgata gacacctccg cgccgacata gcagaaaatg cctgccgtac cgaacacgag
    13801 atgtttgtat tgccatacgc cggttttgcc gtcgtggttg tgcgcgcttt cctcggcggc
    13861 aattttgcgc gcgtcgggca gccggatcat tttcacgaaa acggcaagga taatcagcag
    13921 ccccgccagt cccaaatagg gaatctgtac ggaagaaatc tgttccgcct tgctgacggt
    13981 ttgggttgcg tccgccagaa tcaggaacgc gccgatttgc ggcgcaatgg tcgtacccaa
    14041 agcgttaaac gcctgaacca gcgtcagtgt tgccgattcc ttgccgggtt tcgccagcag
    14101 ggtaacataa ggattaccgg cgacctgaag cagcgttacg ccggaagcca aaataaacaa
    14161 cgcgcccaaa aataccgcgt aagaatggct acccgcagca ggataaaaca gcaggcatcc
    14221 gaccgccgtc agcagaaagc cgccgataac gccgtttttg tagccgactt tgccgacaaa
    14281 agcccccatc gggatggaca tcaccgcata ggcggtaaag aaacagaatt ggatcagcat
    14341 cgcctgaacg taagacaggt cgaaaatttc tttcaaatga gggataagga tgtcgttcat
    14401 gcaggtaata aaacccatca tgaagaacag cgtggtcaag acgaccaatg gggacgtatg
    14461 attgttttgt gattgcgcag acatgatttg cctttcgtgg tgtcggaata ttacccgttt
    14521 caagaaaata gaaattgaca tttcttaacg gcaatgcaaa tcatactgat atttgaacat
    14581 tttgcaaact ttatcggatg caggcaatgc gccgatctga aattcaagca gcagaaatta
    14641 atattttaca gttgcttgca tagattttaa tagattttaa aaaattccgg cggacttgca
    14701 aaaaccgttc ccaataaaat gaaaatccgc cgccgtatcc ggaaaattcg gaaatgccgc
    14761 atcgtggcat caaaaccgga gcggaaagaa tgtcgtatgc ccgacaaaaa aggggcatac
    14821 ggctgtgccg tattttttgc accgtcctcc gcccttcggc gggggcgtga aaata
//
LOCUS       DAXHKX010000028        14756 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHKX010000028 DAXHKX010000000
VERSION     DAXHKX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.028"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(57..677)
                     /gene="galE"
                     /locus_tag="ACFMWZ_000733"
                     /pseudo
     CDS             complement(57..677)
                     /gene="galE"
                     /locus_tag="ACFMWZ_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(756..1100)
                     /locus_tag="ACFMWZ_000734"
     CDS             complement(756..1100)
                     /locus_tag="ACFMWZ_000734"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC5934728.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(1093..1287)
                     /locus_tag="ACFMWZ_000735"
     CDS             complement(1093..1287)
                     /locus_tag="ACFMWZ_000735"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC5934729.1"
                     /translation="MRKVFHYEQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNA
                     VPPLLAKAIAESILKMSENE"
     gene            complement(1265..2095)
                     /locus_tag="ACFMWZ_000736"
     CDS             complement(1265..2095)
                     /locus_tag="ACFMWZ_000736"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC5934730.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKTSRLYYRRYAQSFSL
                     "
     gene            complement(2126..4399)
                     /locus_tag="ACFMWZ_000737"
     CDS             complement(2126..4399)
                     /locus_tag="ACFMWZ_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC5934731.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQNQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4463..5113)
                     /locus_tag="ACFMWZ_000738"
     CDS             complement(4463..5113)
                     /locus_tag="ACFMWZ_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC5934732.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5110..5907)
                     /locus_tag="ACFMWZ_000739"
     CDS             complement(5110..5907)
                     /locus_tag="ACFMWZ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC5934733.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5907..7070)
                     /gene="ctrB"
                     /locus_tag="ACFMWZ_000740"
     CDS             complement(5907..7070)
                     /gene="ctrB"
                     /locus_tag="ACFMWZ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC5934734.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7085..8260)
                     /gene="ctrA"
                     /locus_tag="ACFMWZ_000741"
     CDS             complement(7085..8260)
                     /gene="ctrA"
                     /locus_tag="ACFMWZ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC5934735.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8395..9528
                     /gene="neuC"
                     /locus_tag="ACFMWZ_000742"
     CDS             8395..9528
                     /gene="neuC"
                     /locus_tag="ACFMWZ_000742"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC5934736.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALEVSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            9532..10218
                     /gene="neuA"
                     /locus_tag="ACFMWZ_000743"
     CDS             9532..10218
                     /gene="neuA"
                     /locus_tag="ACFMWZ_000743"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC5934737.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10219..11268
                     /gene="siaC"
                     /locus_tag="ACFMWZ_000744"
     CDS             10219..11268
                     /gene="siaC"
                     /locus_tag="ACFMWZ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HFC5934738.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            11420..14533
                     /locus_tag="ACFMWZ_000745"
     CDS             11420..14533
                     /locus_tag="ACFMWZ_000745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC5934739.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLYPPFHLHPNVKTSSIIDLFNDIPENVSCRNTPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSAEDSIIFTHPLQLEMYRLANNDIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSESEGFPYIFMEGMVYDIPIVVYDFKYGAND
                     YSNYNENGCVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKSSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
BASE COUNT         4368 a         3325 c         2951 g         4112 t
ORIGIN      
        1 ccgataaagc ccgcaccgcc ggtaacgagg atggtttttt tgtttgcggt ttgcatttaa
       61 tccccatatc cgttggggtt gcggctgacc caacgccacg aatcttccat catttgctgc
      121 aagccgcgtt tggtttccca gccggtttgt tgtttggtat gggacgggtc ggcaaaggaa
      181 cacgccaaat caccggcgcg gcgcggtttg atttcaaatg gaatggttaa acccgaagcc
      241 gcttcaaagg cacggatgat ttccaaaacg gaataggcgc gccccgaacc caagttgaac
      301 aaatgtacgc cggcaacgcc gccttttgcc ttcattgccg cgatatgccc ttctgccaaa
      361 tccatcacat gaatgtagtc gcgcattccc gtaccgtcgg gggtcggata atcgccgccg
      421 aataccgaca gttgcggcag cctgcccgaa gccacttggc agatataggg caaaaggttg
      481 ttgggaatac cgttgggctg ttcgccgatc aagccgcttt cgtgcgcccc aatcgggttg
      541 aaataacgca acaaaatcac gctccaacgc ggatcggcct tttggatgtc ggttaacatc
      601 cgttccacca tcgctttgga tgcgccataa ggattagcgg tatcgcccgg gcgcatatct
      661 tccgtatagg gcattttacg tatcatggcg cgacgcatta aagtaaacgc caaagcgcgt
      721 ttatactggt tatccagttt ccctatattt ttacgttaca aatcaagttg ttggtattca
      781 tcgtgaaaat agaatttttc ggcataacgc tgactcataa aaccggcatg cgggctgcct
      841 gaaaaaatca ttgcgacatc atcgtcattt aatgtttcat ggttgttttc tattaatgct
      901 aaagcaattt ggtaattaat tgccaaaata tcgttagtaa tcacacgata gccgcgtttt
      961 ttggcttcgt aggcaaagga acagcctcca ctaaatacat ctgcaactgt atctacatca
     1021 gacggaagct ggtcacaaat ccaggaagct attttctctt tattaccgat atagttaatt
     1081 ttcggatatt gcttattcat tttcactcat ttttaaaata ctttcagcaa tagcttttgc
     1141 caataaaggc ggtacggcgt taccaacctg ctgctgctgg gcaattttgc tgccgcaaaa
     1201 aataaaatta tcagggaaag attgtaaggc agctaattca cgaacggtta acgcccgatt
     1261 ctgttcatag tgaaaaactt tgcgcatatc tcctgtaata caaacggctg gttttgttgc
     1321 tgttgtaacg gatgtattta cggatatcac ctgttttcgg acgtaatggt tcaggaatat
     1381 cgttacggtt acctccattt ttaacaaatg ccattttttc taacatttgt gccgaatgat
     1441 tcatagcttc atgatttgca acgtgtggat tgctttcgcc agcagccagt tttggaaaat
     1501 gtcctattgc tgatccaaca gtctgatggg aaatctgcaa aggttcggga aaggaaattt
     1561 tgcctttatc cctcctcccg ataaatatca ctcggctacg tatctgagga acaccgaaat
     1621 cggctgcact cagtatctta cattccaccg aataaccgat attctgaaat gcttgaataa
     1681 tctcaatacg tgttttacct gaattgtgtg tatagagtcg cgctacattt tccataacaa
     1741 aaaaatatgg ttggacaatt ttaactattc ggacaaactc tttaaataaa tggttgcgtg
     1801 ggtcatctgt aaatgtccgt ccaatctttc ctgccatact aaaaccttga caaggtggtc
     1861 ctccaataat caaatcaact gcttgtccgt taagacaatt gattaaatct tgttcggtta
     1921 gtgtggttaa atctttttgc agtaattgat gatgggggaa gttggtacgg taagtctgac
     1981 aataatcaga ctccatttca acagaaagca attgttggaa tccggcttgt tcaaaaccca
     2041 aggataggcc tcctgctcct gagaaaaggt caatataggt aagtggtgtg tgcataagtc
     2101 aaaatccata aactcttcga ttattttacc gcttcagctt cgcaaacgca tccgccatcg
     2161 ccgaattggc tggggcgcgg tcgttgcgtt ggggtttgcg gccggctgtc cgttcgcggc
     2221 tgcggttttc agacggcatt ttatgttttg cgccgcccgg ttcgtcatcc aagcgcatgg
     2281 tcagcgcgat gcgtttgcgt gcagcatcga cttccagcac tttcactttc accacgtcgc
     2341 cagctttcac cacttcgcgc gggtcttgga cgaacttgtt ggacagggcg gagatgtgca
     2401 ccaagccgtc ctgatggacg ccgatgtcca cgaacgcgcc gaagttggcg acgttggaaa
     2461 ccacgccttc gagtatcata ccgacttgca agtcgctgat ttcgtggata ccttcggcaa
     2521 acgatgccgt ctgaaactcg ccgcgcggat cacggccggg tttttccagt tcggacagga
     2581 tgtccaaaat ggtcggcagg ccgaagcgtt cgtcggtgaa gtcggacgct ttgatttgct
     2641 tcacgcgctc gcggttgccg atgagttcgg cggcgctaat gccttgttgc gccagcattt
     2701 tggcgacgac gggataggct tcggggtgga cggcgctcgc gtccaacggc tctttaccgc
     2761 cgttaatccg caaaaagcct gccgcctgct cgaaggtttt ttcgcccaaa cgcggtactt
     2821 tcagcaattt tttgcggctg tcgaacgcgc cgttttcatc gcggtaggca acgatgtttt
     2881 gggcaagggt ttgattcaag ccggaaatcc gcgccaagag cggggcggag gcggtattca
     2941 cgtccacgcc gacggcgttc acgcagtctt cgaccactgc gtccagcgat ttggcgagct
     3001 ggttttggtt cacatcgtgc tgatactggc ccacgccgat ggatttaggg tcgattttga
     3061 ccaactcggc aagcgggtct tgcagcctgc gggcgatgga caccgcgccg cgcagggaaa
     3121 cgtccaagtc ggggaactcg cgcgccgcca gttcggacgc ggaataaatc gacgcgccgg
     3181 cttcggacac gacgatttta tgcagcccca ttttcgacat ttcacgcacc agttcgcccg
     3241 cgattttgtc ggtttcacgg ctggcggtgc cgttaccgat ggcgatgagc ttcacgccgt
     3301 gctgtttaat caggcgcgac agcgttgcca acatattgtt ttcttgatgc aaatagacga
     3361 tgacggtatc cagcagcttg ccggtgtcgt ccaccacggc gcatttcacg ccgttgcggt
     3421 agccggggtc gagacccagc gtggtcagcc gtccggcggg cgcagcaagc agcaagtctt
     3481 tgagattgcg ggcgaacacg gtaatcgcgt cggtgtcggc ggcttctttc aaacgattga
     3541 gagcttcaag ttccaacgac aaaaagattt tcgcgcgcca agtcaggcgc acggtgtcgc
     3601 gcagccattt gtggccgtct gaaactttga agcggcgggc gatgatttgc tcgtattcgc
     3661 tttgctgcgt aatcggcgtg tcgtcgggct gatatttgag cgcgatgttc aacacgcctt
     3721 cgttgcgtcc gcgcaaaacc gctagcgcac gatggctggg catcgcacga ataggttcgc
     3781 ggtggtcaaa ataatcgctg aatttttcgc cttcggtttc tttgcctcca acaacttgcg
     3841 cgtggatttc ggcttcgttc cacagcttgt cgcgcagcgt gccgatgagt tccgcgtctt
     3901 cggcaaactg ctccatcaga atcgcacgcg cgccgtccaa cgcggctttg gcatcgggga
     3961 cgttttcgtt caggtagcct tgtgcggcgg cttccacgtc ctgcgactgc tcggcaagca
     4021 acacgtccgc cagcggctgc aaaccgtgtt cgcgcgcgat ttgcgctttg gtgcggcgtt
     4081 tgggtttgta gggcagatac aggtcttcca gcgcggtttt gttatcggcg gcttcgattt
     4141 gcgccctgag gtcgtctgaa agcttgcctt gctcttcaat gctttttaaa acaacggctt
     4201 tgcgctcttc caactcgcgc agatattgca gccgctcggc aagccggcgc aactgcgtat
     4261 cgtccaaccc gcccgtcgct tccttgcggt agcgggcgat aaacggcacg gtcgcgccgt
     4321 cgtccaaaag ctcgacggcg gcggtgattt gcgcggcagt cgcggagagt tcttgggaaa
     4381 gaatttgagt aatgttcatc aatagaattc caacggacag gccgtctgaa atttcagacg
     4441 gcctgattta aaaacaatcg ctttaaggca gcgaattata atattcgtag gctttgtcca
     4501 tatcttcaaa ctggtacata tgcccttttt ccagcaccat cgcattatcg caatattgct
     4561 tcatggcgct gtggctgtgc gacaccaaga tgatggaacg gtctttgcgc ttttcaaaca
     4621 actcgtactt acatttatcg gcaaaacgcg agtcaccaac tgcaatcact tcgtcaatca
     4681 aatagcaatc aaactctacc gccaacgaca aagcaaatgc caaacgcgcc ttcatacctg
     4741 aagaataacg cttgaccggc tcgtaaagat attgccccaa ctcggaaaac tcttcggtaa
     4801 agttcttcac ataatcgata tcgacattgt aaatccggca gatgaaacgc aaattgtcca
     4861 taccggtcag actgccttga aacgcaccgg agaatgccaa aggccaagaa atactcattg
     4921 tccgcttgat ttcacccgtg gtcggcggct caacgccact gatcaaacgg atgagcgtcg
     4981 atttacctgc accgttgcgg ccgagaatac cgattttctc gcccttctcc attttgaagc
     5041 taatatcgtg caagactgtc cgccaacctt ggcgggtcag atagcgtttg gaaacgtgtt
     5101 caactgaaat cattgcggct cgactccttt actgaattta ctgaccatcg ccaagccaaa
     5161 caacaacaac accagattgc acaatacgat ataccaagga ttttcatagg taattacatc
     5221 gctgccaaaa tatccggcac ggaacatttc tgtgccatgc accatcggga tcatcaatgc
     5281 atactcttgc actttcggag gcaaattatg tacaaaaaag aatgcacccg ataacggcat
     5341 catcacaaaa gtcaatgtgc cccaaatctt gccaaacggc tcgaaattaa aggcaatcga
     5401 acaaatcacc aaacccaaac caatcgcaaa aaaagccatc aaaagccaag ccatcagcat
     5461 ataaaacata tctgccggca tttcaatcca gccaatcgca atcaataccg ccataatcac
     5521 aatctgcgca atggttgcac cagcaatttc caaaatcatg cgcgccaaga tggtatccaa
     5581 aactcttaca ttgcggtgat aaagcaagct ggcatttgaa gaaatcgacc caactgcccg
     5641 ttttgaggca ttacgccaca tcatcaacat cggatagcca gtaatcgcaa atgcgacaat
     5701 attcaaagtt gaatatcggt ctgcccttaa aaatttccac atcaagacga taacgaatgt
     5761 catcagcaac ggctcaacaa acagccataa aaagccaata ttattgcgac cgtaacgggt
     5821 gataatttcc cgcatcaaca gcgcaccgat tacgcgcctt tgaatggcta aagattccca
     5881 aaatgatgtt ttatgcaagg ctttcatcag tttttatgct cacgaatgct ggcagtcaac
     5941 aggctcaaaa taccataaac catcaagccg ataatcagag tggcaacaat gttgtataac
     6001 cgtttaggct catgtgccaa atccggcagg ctcggttgcg agatcacttc caaataaagc
     6061 tgctgacggt ctgcttcaac cttggcactt tccaaagaag tcatggcggc tgccaactgc
     6121 tgctctgcca actggttttc caaatacaca cgctgatatt cggcagcctg attagacaac
     6181 gaagaatgcc caccgcccga aatggcacgt aactgttggt caatttcttt acgcaagctc
     6241 tgctcacgcg cctgcaaacc cggaatctgc ggattctccg gagtgactgc tttcacctga
     6301 tccagctggg tttgaatcac aatcaattca tcttgcagct tggaaaccaa ccccatttgc
     6361 acttccgatt gcgctttcaa atcaaaaacg ccattggcaa tccggtaatc cgtcagattc
     6421 tgagaggctt cctttacccg ctctgccgcc gttttcacta cttcttccgc atagcgcacc
     6481 gtatcagcac gtgcacgatc gttcaactgg ttaatcaatg cttcaccttg ttttaacaaa
     6541 gcctcattga ttttcttaga ttccagcgca tcaaaggaag ttacattcaa cgtggaaata
     6601 cccgaaaccg tatcaaaatt gatcatcacc tgatttttat agtattgata aaaagcctct
     6661 tcctcgccac ggaacccaaa cccattaaag cggctgaacg catcaccttt ggtttcataa
     6721 aactcacgca ccggcaagat tttacgcagt tcatccaaag acgagcgcga acgcatatac
     6781 tccccaaccg tgtaaatatc atcttgcgca cgggcaaaac ctgtgccctg caaaatggca
     6841 cccaggccat tgagagaaga ttggctttta ggcgagcgca ccacaaagct cgattgcgac
     6901 gtaaaacgat cggaagcgaa gaagccgaaa tacaccaacg aaattaccgt agggataatc
     6961 accgttaccc aaaataaagg gcttagcttt ttaatccaac ttttcttttt cggcttttta
     7021 cgctcggctt tggtttcagt ggcaactgcc acaggaagtt gctcagacat cttacttact
     7081 cacattaatt agttaaatta ttaatactgt tcgcgccact ggtaaccggc gagaacacaa
     7141 acgacaaaaa tttctgcact tcagccaacg gcgcattcga cacatacaat acatctttat
     7201 tcttcacagg aaagcgctgc atagaaaata gcgaatgcgc atcagccata ttcacacgat
     7261 ataccgttgg aatctctgcc tcactgccat aaccttgagc aatccattta tcctgacgtt
     7321 ctgccggcaa ttccaccaat ggcgtatagc ggaacacaaa cacaccacgc gcatcagaac
     7381 ggcgatcttg caaaccgccc atacggccaa tggcttcaga aagcgataag cctctggctg
     7441 aaaaaccgat ttcttgtgtt ctccccaccg cacccataga cgtaaaggta tagggattgg
     7501 taatcatggt aaccacatca ccgcgacgca gcaaaatatt ttgtcgcgga tttgcaacta
     7561 aatcttccaa ggcaacagtt cgtactacat tgccacgtgt cagctgcaca ttcgtatcct
     7621 gcacatttgc cgttgaacca cctaccgcag ccaccgcatc caacacacgc tcaccggctg
     7681 ccgtcagcgg catacgcaca ctattcccag cacgaatcac cgacacattc gccgcattat
     7741 tctgcaccaa acgcaccatc acttgtggct gattggccat ttttttcagg cggcctttaa
     7801 taatttcctg aacctgacca ggcgttttac cgaccaccga aatatcgcca acaaacggca
     7861 cagaaaccgt accacgtgcc gtgaccaact gctctggcaa cttagtttga tgcgcactac
     7921 ccgagcccat cgaagaaagg ccaccaccaa acaatactgc cggcggcgct tcccaaatca
     7981 taatatccaa tacatcacca atatttagcg taccagccga agcataacca tcgccaaact
     8041 gagtgaatga ctgatttatc tgagccttat ataataactg agcaaccgta tgattcacat
     8101 caatcagctc cacttcagga atttgaactt cagattgttg ccctaaagag acaatttttt
     8161 ttgcgctggg gcctgatgaa ggaatcgcag agcatcctac aattaaactt ccacacaata
     8221 ataatactgc gtgacgaata taaaatttca ctttaaacac aagccaaatc ctaatataat
     8281 tataaatggc ctaattatag cacttaatcg aaataaattt atgagtacgt agagtataat
     8341 tagtattctt ctttccaact tccttatact tatatactta tagattctaa aatcatgaaa
     8401 agaattcttt gcattacagg taccagagcc gacttcggca agctaaaacc tttattagcc
     8461 tatattgaaa atcacccaga ccttgaattg catttgattg taactggtat gcatatgatg
     8521 aaaacatatg gcagaacctg caaggaagta actcgagaaa actatcaaca tacatatctg
     8581 ttttcaaatc aaatccaagg tgaaccaatg ggtgccgttt taggcaatac cattacgttt
     8641 atctctcgtc tatctgatga aattgaacct gatatggtca tgattcacgg cgaccgttta
     8701 gaagcactag caggcgcaac tgtaggtgca ttaagcagcc gtttagtttg ccatatcgaa
     8761 ggtggtgaac tatctggtac agtagatgac tccattcgtc attctattag taaactttct
     8821 catatccact tggtagcaaa tgaacaagct gtcactcgcc tagtgcaaat gggagaaaaa
     8881 agaaagcata ttcacatcat cggctccccc gatttagatg ttatggcctc ttccaccctc
     8941 ccatccttag aagaagtcaa agaatattac ggtttaccat acgaaaatta tggtatttct
     9001 atgtttcacc ccgtgactac agaagcacat ttaatgccac aatatgcggc ccaatatttc
     9061 aaagcattag aagtaagtgg ccaaaatatc attagcatct accctaataa tgacactggc
     9121 actgaaagta ttctgcaaga attattaaaa tatcaatctg ataaatttat tgcatttcct
     9181 tccattcgat ttgaatactt tttagtctta ttgaaacatg ctaaatttat ggtcggcaac
     9241 tcaagtgcag gtattagaga agctcctctc tacggtgtcc cttcaattga tgttggtaca
     9301 cgccaaagta accgccatat gggaaaatct attattcata cagattatga aactaaaaat
     9361 atctttgatg cgattcaaca agcatgcagt ttaggcaaat ttgaagcaga tgataccttt
     9421 aatggcggag atactcgcac cagcacagaa agatttgctg aagtaatcaa caatcctgaa
     9481 acgtggaatg tttctgctca aaaacgtttt atcgatttga atctttaaat tatggaaaaa
     9541 caaaatattg cggttatact tgcgcgccaa aactccaaag gattgccatt aaaaaatctc
     9601 cggaaaatga atggcatatc attacttggt catacaatta atgctgctat atcatcaaag
     9661 tgttttgacc gcataattgt ttcgactgat ggcgggttaa ttgcagaaga agctaaaaat
     9721 ttcggtgtcg aagtcgtcct acgccctgca gagctggcct ccgatacagc cagctctatt
     9781 tcaggtgtaa tacatgcttt agaaacaatt ggcagtaatt ccggcacagt aaccctatta
     9841 caaccaacca gtccattacg cacaggggct catattcgtg aagctttttc tctatttgat
     9901 gagaaaataa aaggatccgt tgtctctgca tgcccaatgg agcatcatcc actaaaaacc
     9961 ctgcttcaaa tcaataatgg cgaatatgcc cccatgcgcc atctaagcga tttggagcag
    10021 cctcgccaac aattacctca ggcatttagg cctaatggtg caatttacat taatgatact
    10081 gcttcactaa ttgcaaataa ttgttttttt atcgccccaa ccaaacttta tattatgtct
    10141 catcaagact ctatcgatat tgatactgag cttgatttac aacaggcaga aaacattctt
    10201 aatcacaagg aaagctaaat gcaaaacaac aacgaattta aaattggtaa tcgttcagta
    10261 ggttacaacc acgaaccatt gattatctgt gaaatcggca tcaatcatga aggctcttta
    10321 aaaacagctt ttgaaatggt tgatgctgcc tataatgcag gcgctgaagt tgttaaacat
    10381 caaacacaca tcgttgaaga cgaaatgtct gatgaggcca aacaagtcat tccaggcaat
    10441 gcagatgtct ctatttatga aattatggaa cgttgcgccc tgaatgaaga agatgagatt
    10501 aaattaaaag aatacgtaga gagtaagggt atgattttta tcagtactcc tttctctcgt
    10561 gcagctgctt tacgattaca acgtatggat attccagcat ataaaatcgg ctctggcgaa
    10621 tgtaataact acccattaat taaactggtg gcctcttttg gtaagcctat tattctctct
    10681 accggcatga attctattga aagcatcaaa aagtcggtag aaattattcg agaagcaggg
    10741 gtaccttatg ctttgcttca ctgtaccaac atctacccaa ccccttacga agatgttcga
    10801 ttgggtggta tgaacgattt atctgaagcc tttccagacg caatcattgg cctgtctgac
    10861 cataccttag ataactatgc ttgcttagga gcagtagctt taggcggttc gattttagag
    10921 cgtcacttta ctgaccgcat ggatcgccca ggtccggata ttgtatgctc tatgaatccg
    10981 gatactttta aagagctcaa gcaaggcgct catgctttaa aattggcacg cggcggcaaa
    11041 aaagacacga ttatcgcggg agaaaagcca actaaagatt tcgcctttgc atctgtcgta
    11101 gcagataaag acattaaaaa aggagaactg ttgtccggag ataacctatg ggttaaacgc
    11161 ccaggcaatg gagacttcag cgtcaacgaa tatgaaacat tatttggtaa ggtcgctgct
    11221 tgcaatattc gcaaaggtgc tcaaatcaaa aaaactgata ttagttaata ataaaataga
    11281 ttaagctatt cttaaattca gaatattgct tatctatatt aaaaatttct aatttttaag
    11341 gttctgattg aaatcagaac cttatttcaa ctattacttt ttactcataa tcgaattata
    11401 tactttagga ctttataata tggctgttat tatatttgtt aacggaattc gggctgtaaa
    11461 tggccttgtt aaatcatcta tcaatactgc aaacgctttt gctgaagaag gactggatgt
    11521 tcatttaatt aattttgttg gcaatattac tggagcagag catttatacc ccccattcca
    11581 cttacatccc aatgtcaaaa cctccagcat catagattta tttaatgaca ttccagaaaa
    11641 tgttagctgc cgaaatactc ctttttattc tattcatcaa caattcttca aagctgaata
    11701 tagtgcccac tataagcatg ttttgatgaa aattgaatct ttattatctg cagaagatag
    11761 cattatcttc actcatcctc ttcaactgga aatgtatcgt ttagcgaata atgatatcaa
    11821 gtcaaaagcc aaactaattg tacaaattca tggtaattat atggaagaaa tccataacta
    11881 tgaaattttg gcacgaaata tcgattatgt tgactatctt caaacggtat ctgatgaaat
    11941 gctggaagaa atgcattccc atttcaaaat caaaaaagac aaattagttt ttattccaaa
    12001 catcacttat cccatttcat tagaaaaaaa agaagctgat ttctttatta aggataatga
    12061 agacatcgat aatgctcaga aatttaaacg tatctctatt gttggcagca ttcagccaag
    12121 aaaaaaccaa ttggatgcca ttaaaatcat caataaaatt aaaaatgaaa attacatttt
    12181 acagatatat ggcaaatcta ttaataaaga ttactttgaa ttaattaaaa aatatattaa
    12241 agacaataag ttacaaaacc gtatcttatt caaaggtgaa tcttccgagc aggaaattta
    12301 tgaaaataca gatatcctga tcatgacatc agaaagtgag ggatttccat atatatttat
    12361 ggaaggcatg gtgtatgata ttccaatcgt tgtatatgat tttaaatatg gagcgaatga
    12421 ttacagtaac tataatgaaa atggttgtgt ttttaaaact ggtgatattt ctggaatggc
    12481 aaaaaaaata attgagctat taaataaccc agaaaaatat aaagaattag ttcaatataa
    12541 tcacaatcgc ttcttaaaag aatatgcaaa agatgtggtt atggctaaat atttcactat
    12601 tcttccgcgc agctttaata acgtatcatt atcgtctgct ttcagccgaa aagaattgga
    12661 cgaattccaa aatattactt tttctattga agattctaat gatttagctc atatttggaa
    12721 tttcgagcta accaatcctg cacaaaatat gaattttttt gctttagttg gcaagcgaaa
    12781 atttccaatg gatgctcata tccaaggaac acagtgtacg attaagatag ctcataaaaa
    12841 gacagggaat ttattgtcgc ttttactaaa aaaacgaaat cagttgaatt tatcaagggg
    12901 atatacctta attgcagaag ataatagcta tgaaaaatat attggagcaa tatctaataa
    12961 aggtaacttt gaaattattg caaataaaaa gagctcatta gttactataa acaaaagtac
    13021 cttagagttg catgagattc cccatgaact acatcagaat aaattactga ttgctttacc
    13081 caacatgcaa acgcctctaa aaattactga tgataattta atacctatcc aagcctccat
    13141 aaaattagaa aagattggaa atacttatta cccatgtttc ttgccatctg gcatatttaa
    13201 taatatctgc ttagattacg gtgaagaatc caaaattatt aattttagta aatattctta
    13261 taaatatatc tatgactcaa ttcgtcatat tgagcaacat acagatatat cggatattat
    13321 cgtttgcaat gtttattctt gggaacttat tcgtgcctca gttattgaga gccttatgga
    13381 atttaccgga aaatgggaaa aacactttca gacttctcct aaaattgatt atcgatttga
    13441 tcatgaaggt aagcgttcga tggatgatgt cttttcagaa gaaacattta ttatggaatt
    13501 tccgcgtaaa aatggtatag ataagaaaac agcagccttc caaaatatac caaacagtat
    13561 tgtaatggag tatccgcaga ccaatggtta cagtatgcgc agtcattcac tgaaaagtaa
    13621 tgtagttgcg gcaaaacatt ttcttgaaaa attaaataaa attaaggtag atattaaatt
    13681 taaaaagcat gaccttgcaa acatcaaaaa aatgaatcga attatttatg agcatttagg
    13741 cattaacata aatatcgaag catttctaaa accacgatta gaaaaattta agcgtgaaga
    13801 aaaatatttt catgatttct tcaaaagaaa taattttaaa gaggtaattt ttccaagcac
    13861 ttattggaat ccaggtatta tttgtgctgc acataaacaa ggtattaagg tatctgatat
    13921 tcaatatgct gccattactc cttatcatcc tgcgtatttt aaatcaccaa aatcacatta
    13981 cgttgctgat aaattgttct tatggtctga atattggaat catgagcttt taccaaatcc
    14041 aacacgagag attggttctg gtgccgcata ttggtatgca ttagatgatg tgagattttc
    14101 agaaaaactg aattatgact atatctttct atctcaaagt aggatttctt cgcgcttgct
    14161 tagttttgca attgagtttg cattaaaaaa tcctcaacta cagcttttat tttctaagca
    14221 tccagatgaa aatatagatt taaagaacag aattattcct gataatctta taatctccac
    14281 ggaatcttct atacaaggca tcaatgaatc tcgcgttgct gtaggtgttt attcaactag
    14341 cttatttgag gcattagcat gcggcaaaca aacttttgtt gttaaatatc cgggatatga
    14401 aattatgtca aatgaaatag attcagggtt attctttgca gtagaaacac ctgaagaaat
    14461 gcttgagaaa acaagcccga attgggtggc tgtggcagat attgaaaacc agttttttgg
    14521 ccaagaaaaa taatttttaa gttaatcttt aaaagtgcaa tatttttctt attttaaata
    14581 ttgcacttta ttcttttaat cataggaact cacatgtatt ctgaacaggg aattaataat
    14641 actattaaca tatctactac atctttaacc aatgccacgc aactaactgt gatcgggaat
    14701 aataactctg tatatatagg taataattgt aaaatcgtca gctccaatat tagatt
//
LOCUS       DAXHKX010000029        14602 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHKX010000029 DAXHKX010000000
VERSION     DAXHKX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.029"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..79)
                     /locus_tag="ACFMWZ_000746"
     CDS             complement(<1..79)
                     /locus_tag="ACFMWZ_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC5934740.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="ACFMWZ_000747"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="ACFMWZ_000747"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC5934741.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="ACFMWZ_000748"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="ACFMWZ_000748"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC5934742.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAVADKQLGNNRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="ACFMWZ_000749"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="ACFMWZ_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC5934743.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRRDE"
     gene            4807..5871
                     /gene="fba"
                     /locus_tag="ACFMWZ_000750"
     CDS             4807..5871
                     /gene="fba"
                     /locus_tag="ACFMWZ_000750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC5934744.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVGAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            6029..6874
                     /locus_tag="ACFMWZ_000751"
     CDS             6029..6874
                     /locus_tag="ACFMWZ_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC5934745.1"
                     /translation="MNRTAFCCLFLTTALILTACSSGGGGSGGGGVAADIGAGLADAL
                     TAPLDHKDKGLKSLTLEDSISQNGTLTLSAQGAERTFKAGNKDNSLNTGKLKNDKISR
                     FDFVQKIEVDGQTITLASGEFQIYKQNHSAVVALQIEKINNPDKIDSLINQRSFLVSS
                     LGGEHTAFNQLPGGKAEYHGKAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVE
                     LAAAELKADEKSHAVILGDTRYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIG
                     IAGKQ"
     gene            7012..7689
                     /gene="tsaB"
                     /locus_tag="ACFMWZ_000752"
     CDS             7012..7689
                     /gene="tsaB"
                     /locus_tag="ACFMWZ_000752"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC5934746.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEVGSRQSALI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRARL"
     gene            7686..8126
                     /gene="rimI"
                     /locus_tag="ACFMWZ_000753"
     CDS             7686..8126
                     /gene="rimI"
                     /locus_tag="ACFMWZ_000753"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC5934747.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSLSEQVFL
                     AEKDGGLAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRATDGQTEDAVLMEKIC"
     gene            8120..8857
                     /locus_tag="ACFMWZ_000754"
     CDS             8120..8857
                     /locus_tag="ACFMWZ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC5934748.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8919..9560
                     /gene="pyrE"
                     /locus_tag="ACFMWZ_000755"
     CDS             8919..9560
                     /gene="pyrE"
                     /locus_tag="ACFMWZ_000755"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC5934749.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9634..10068
                     /locus_tag="ACFMWZ_000756"
     CDS             9634..10068
                     /locus_tag="ACFMWZ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC5934750.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10065..11375
                     /gene="argA"
                     /locus_tag="ACFMWZ_000757"
     CDS             10065..11375
                     /gene="argA"
                     /locus_tag="ACFMWZ_000757"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC5934751.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11471..13486
                     /locus_tag="ACFMWZ_000758"
     CDS             11471..13486
                     /locus_tag="ACFMWZ_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC5934752.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLALFAL
                     DLNVMELTVMRRQPQQFDSDGINVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDDVPYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(13600..14556)
                     /locus_tag="ACFMWZ_000759"
     CDS             complement(13600..14556)
                     /locus_tag="ACFMWZ_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC5934753.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFTL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
BASE COUNT         3239 a         4511 c         3957 g         2895 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatat agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcgt gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gtaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcacagg aaaaagtgtt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgttga cgtggtcttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctcattc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agataccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcggcga cggtggcgtt cagttttccg gcgaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg catccgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg caacgcaaga agctcaaatc gtacaaaccg
     2281 gcgtgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatatc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caataccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcgac gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgcct tctttaggca gcttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcggc gagtttgtag ccgttgcctt ttttggtgat gacgtgcaga
     2761 agctgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt atagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcagttca gggcttcaaa cgcctgacca gccgtcatgg ctccgtcgcc gatgatggcg
     3181 acgctgcggc ggttgttgcc caactgtttg tccgcaacgg ccatgcccaa ggccgcgccg
     3241 atggatgtgg aagaatgccc tacgccgaac gcgtcgtatt ccgactcgct gcgttttggg
     3301 aagccagcta aaccgccgta ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgtcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca gattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gcacgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ctagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggtcaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cccgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gtttggtgta aatctgcgtg
     3781 gtcgaaaggc tgctgtgtcc gagcagctcc tgcaccgccc ggatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcatagct gtggcgcatc atatgcggcg aaacgtgtct gccgtctccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccggtcagc ggaacctgac gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatat acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagc tcgaacaggg cgtgatcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt tgaccagcca ggcgcaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgccgc
     4441 gacaagtcct gcggcacgcc gccttcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgca accgtgtgtt ccgacttacc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgtccca
     4621 cacaaatatc aaaaaaccag tgaaaacatt ataataaacg acacaccaaa acctcgaacc
     4681 acccaaaatc cgacccgtcc gctcgccgtt atcgttaaca taaaatcaat gtagcggttt
     4741 gtagtttttt gccgcaaaaa acattataat ccaacttatt tacccccact gcccaaggag
     4801 acaaaaatgg cactcgtatc catgcgccaa ctgctcgacc acgccgccga aaacagctac
     4861 ggcctgcccg cgttcaacgt caacaacctc gaacaaatgc gcgccattat ggaagccgcc
     4921 gaccaagtcg gcgcacccgt catcgtccaa gcatccgccg gcgcgcgcaa atacgcgggc
     4981 gcgccgtttt tgcgccacct gattctggca gcagtcgaag aatttccgca catccccgtc
     5041 gtgatgcacc aagaccacgg cgcatcgccc gacgtgtgcc aacgctccat ccaactgggc
     5101 ttctcctccg tgatgatgga cggctcgctg atggaagacg gcaaaacccc ttcttcttat
     5161 gaatacaacg tcaacgccac ccgtaccgtg gttaacttct cccacgcttg cggcgtatcc
     5221 gtcgaaggcg aaatcggcgt attgggcaac ctcgaaaccg gcgaagccgg cgaagaagac
     5281 ggtgtaggcg cagtgggcaa actttcccac gaccaaatgc tcaccagcgt cgaagatgcc
     5341 gtgcgtttcg ttaaagatac cggcgttgac gcattggcga ttgccgtcgg caccagccac
     5401 ggcgcataca aattcacccg tccgcccaca ggcgacgtat tgcgtatcga ccgcatcaaa
     5461 gaaatccacc aagccctgcc caatacacac atcgtgatgc acggctccag ctccgttccg
     5521 caagaatggc tgaaagtcat taacgaatac ggcggcaata tcggcgaaac ctacggtgtg
     5581 ccggttgaag aaatcgtcga aggcatcaaa cacggcgtgc gcaaagtcaa catcgatacc
     5641 gacttgcgcc ttgcttccac cggcgcggta cgccgctacc ttgccgaaaa cccgtccgac
     5701 tttgacccgc gcaaatacct gagcaaaacc attgaggcca tgaagcaaat ctgcctcgac
     5761 cgctacctcg cgttcggctg cgaaggtcag gcaggcaaaa tcaaaccggt ttccttggaa
     5821 aaaatggcaa gccgttatgc caagggcgaa ttgaaccaaa tcgtcaaata acaggttgcc
     5881 ggtaaacaaa atgccgtctg aaccgccgtt cggacggcat ttgatttttg cttctttgac
     5941 ctgcctcatt gatgcagtat gcaaaaaaag ataccgcaac caaaacgttt atatattatc
     6001 tattctgtgt atgactagga gtaaacctgt gaaccgaact gccttctgct gccttttcct
     6061 gaccactgcc ctgattctga ccgcctgcag cagcggaggc ggcggaagcg gaggcggcgg
     6121 tgtcgccgcc gacatcggcg cggggcttgc cgatgcacta actgcgccgc tcgaccataa
     6181 agacaaaggt ttgaaatccc tgacattgga agactccatt tcccaaaacg gaacactgac
     6241 cctgtcggca caaggtgcgg aaagaacttt caaagccggc aacaaagaca acagcctcaa
     6301 cacgggcaaa ctgaagaacg acaaaatcag ccgcttcgac ttcgtgcaaa aaatcgaagt
     6361 ggacggacaa accatcacgc tggcaagcgg cgaatttcaa atatacaaac agaaccactc
     6421 cgccgtcgtt gccctacaga ttgaaaaaat caacaacccc gacaaaatcg acagcctgat
     6481 aaaccaacgc tccttccttg tcagcagttt gggcggagaa catactgcct tcaaccaact
     6541 gcccggcggc aaagccgagt atcacggcaa agcattcagc tccgacgacc cgaatggcag
     6601 gctgcactac tccattgatt ttaccaaaaa acagggttac ggcagaatcg aacacctgaa
     6661 aacgcccgag cagaatgtcg agcttgccgc cgccgaactc aaagcagatg aaaaatcaca
     6721 cgccgtcatt ttgggcgaca cgcgctacgg cagcgaagaa aaaggcactt accacctcgc
     6781 ccttttcggc gaccgcgccc aagaaatcgc cggctcggca accgtgaaga taggggaaaa
     6841 ggttcacgaa atcggcatcg ccggcaaaca gtagcatttg aaaaatgccg tccgaacacg
     6901 atagtttacc gttcggacgg catttttgcg ccatactgcc ctaggacacg acggcacggc
     6961 aggcaagccg atttcactat ataatgccgt ctgaaccaac gagagaatgc catgcaagct
     7021 gatttcaacc gccccgtcct cgccgtcgat accggaactt cctatttgtc gctcgcgctg
     7081 cgcgccgacg gcgaaacccg tctgttccat caggaagtcg gcagccgcca gtccgcactg
     7141 atcctgccgg aaatccgcac cctattccga aatgcaggca ttaccgccgc cgatttgggg
     7201 gcgattgttt atgcgaaagg ccccggcgcg tttacgggac tgcgtatcgg tatcggcgtg
     7261 gcgcaaggtt tggcaacgcc gtttgatacg cctttaatcg gcatccccac gctcgatgcc
     7321 gccgcctcgc tgccgccgcc gcaaagctgc atactcgccg ctgcggacgc gcgtatgggc
     7381 gaagtgtttt atgcgtggtt cgacacgctg aaccgccgcc gtttgagcga ttatcaggtc
     7441 gggcgggcgg cagacatccg gctgccggag ggatgtgcct tttcagacgg cataggcagc
     7501 gcgttcgcgt tggaagaaac gccgccgttt tcaggcaaac ccgatatgcc gaccgccgcc
     7561 gacttcctca atctggcggc tgaaggcggt tttcccgccg tcgctgcggc acacgccgag
     7621 ctgctctacg tccgcaacaa aatcgccctg actgccaaag aacaggcgga acggagggcg
     7681 cgcctgtgaa catccgccgc gccgtttatg ccgattgcgc ggcactcgcc gcactcgatg
     7741 ccgtctgcaa cccgtccgcg tggacgcaac gccaatttga gtctgcattg gtttcgctgt
     7801 ccgaacaggt tttcctcgcg gaaaaagacg gcgggttggc cgcctttatc gtttggcaga
     7861 acctgcccga cgaatccgaa ctgcacctga ttgccaccgc gcccgaatgc cgccgccaag
     7921 gaattgcgtc cgccctgctc gaatattggt tcgcacatct gcccgaaggc acgcaacgcc
     7981 tgctgctcga agtccgcgca ggcaacaccg ccgcgcaggc actgtacgcg gcgcacggct
     8041 tcagcattgc gggcaggcgg aaaaactatt accgcgccac cgacgggcaa accgaagatg
     8101 ccgtcttaat ggagaaaata tgttaagcgc gcgctacctc cacctgcacg aagctttggg
     8161 tttgggtccg atgtggctga aacaggccgc tgccgtcctg ccgcccaaaa acacacccgc
     8221 aaccccggca caggcacgtc ctcaaaccgt ccgcgccgcc ccgatccgcc cttcccaacc
     8281 ccataacggt caggcgcggc tcgaaacgat gaaagcgttg gaaaccgccg ccgtacctac
     8341 gcgcaaaccc gcgcctgaaa ccgaaacgcc tctgcccggc ctttcagacg gcatcgcccc
     8401 cgttcccgcc gcttcgggca taaccaaact tgccgtcgtc agcctttgcc caccgattga
     8461 ggatgcggtt tacgggcaac tgttctacgg caaagcgggt gttctgcttg acaacatact
     8521 caaagccgta ggactggatg ccgcctatgt ccacaaaacc tgttgggtga aaaccgccgc
     8581 cgtcggcaac ccgatgccgt ctgaagcggc aatcgaaacc gcgacggttt ccgtcatgca
     8641 ggaactcgac ggctgccgcg cccccgccgt cctattcctc ggacaggctt ttgtccagcc
     8701 tgaacggcaa acgatgattg aaactttgtg cggcagccgt cccttcttca tcatcgacca
     8761 tcccgcccgg ctgttgcgcc aacccgaact taaagcccgc gcctggcagg tgttgaaaca
     8821 gttgaaacgc gccttgcggc aaggcggcgg cagttgaagc gcaccgcacg gggcggtaga
     8881 atcgcaactg cgtcccaata tctgacagaa agcacaaaat gaccgatttc cgccaagatt
     8941 tcctcaaatt ctccctcgcc caaaatgttt tgaaattcgg cgaatttacc accaaagccg
     9001 gacggcggtc gccctatttc ttcaatgccg gcctctttaa cgacggcttg tccacgctgc
     9061 aactggcaaa attttacgca caatccatca ttgaaagcgg catccgattc gatatgctgt
     9121 tcggccccgc ctacaaaggc attattttgg cggcggcaac cgcgatgatg ctggcggaaa
     9181 aaggcgtgaa cgtcccgttt gcctacaacc gcaaagaagc caaagaccac ggcgaaggcg
     9241 gcgtgttggt cggtgcgccg cttaaaggcc gcgtgctgat tatcgacgac gtgatttccg
     9301 ccggcacatc cgtacgcgaa tcgatcaaac tgattgaagc ggagggtgca acccccgccg
     9361 gtgtcgccat cgcgctcgac cgcatggaaa aaggcacggg tgaattgagc gcggttcagg
     9421 aagtggaaaa acaatacggt ctgcccgtcg cccccatcgc cagcctgaac gatttgttta
     9481 ttctgttgca aaacaacccc gaattcggac agttcctcga acccgtccga gcctaccgtc
     9541 ggcagtacgg cgtagaataa aaacaaagca tatgccgtcc gaaccgcctt acgcctcaga
     9601 cggcatcaaa cctgacacac acgaggaaat accatgcccg cctgtttctg cccccactgc
     9661 aaaacccgtc tctgggtcaa agaaacccaa ctcaatgtcg cccaaggctt cgtcgtctgc
     9721 caaaaatgcg aaggactgtt taaagccaaa gaccatctgg caagcacgaa agaacccata
     9781 ttcaacgatt tgcccgaggc tgtttcggat gtcaaactcg ttcaccgcat cggcacgcgc
     9841 gccattggca agaaacagat ttcccgcgac gaaatcgccg gcatcctcaa cggcggtaca
     9901 accctgcacg atacgccgcc cgcaaccgcc gccacccctg ccgccgcacc gcaggttgcc
     9961 gtaccgcccg ccgcgcctgc ccgtcaggaa gggttcaact ggacgattgc aaccctgttt
    10021 gcccttatcg tcctcattat gcagctttcc tacctcgtca tcctatgatc gtgcccgacc
    10081 tctttgtcgc ccacttccgc gaagccgtcc cctacatccg ccaaatgcgc ggcaaaacgc
    10141 tggtcgccgg catagacgac cgcctgctcg aaggtgatac cttaaacaag ctcgccgccg
    10201 acatcgggct gttgtcgcaa ctgggcatca ggctcgtcct catccacggc gcgcgccact
    10261 tcctcgaccg ccacgccgcc gcgcaaggcc gcacgccgca ttattgccgg ggcttgcgcg
    10321 ttaccgacga aacctcgctc gaacaggcgc agcagtttgc cggcaccgtc cgcagccgtt
    10381 ttgaagccgc attgtgcggc agcgtttccg ggttcgcgcg cgcgccttcc gtcccgctcg
    10441 tatcgggcaa cttcctgacc gcccgtccga taggtgtgat tgacggaacc gatatggaat
    10501 acgcgggcgt tatccgcaaa accgacaccg ccgccctccg tttccaactc gacgcgggca
    10561 atatcgtctg gctgccgccg ctcggacatt cctacagcgg caagaccttc tatctcgata
    10621 tgcttcaaac cgccgcctcc gtcgccgtct cgcttcaggc cgaaaaactc gtttacctga
    10681 ccctttcaga cggcatttcc cgccccgacg gcacgctcgc cgaaaccctc tcggcacagg
    10741 aagcgcaatc gctggcggaa cacgccggcg gcgaaacgcg acggctgatt tcgtccaccg
    10801 ttgccgcgct cgaaggcggc gtgcatcgcg tccaaatcct caacggtgcc tccgacggca
    10861 gcctgctgca agaactcttc acccgcaacg gcatcggcac atccattgcc aaagaagcct
    10921 tcgtctccat ccggcaggcg cacagcggcg acatcccgca catcgccgcc ctcatccgcc
    10981 cgctggaaga acaaggcatc ctgctgcacc gcagccgcga atacctcgaa aaccacattt
    11041 ccgaattttc catcctcgaa cacgacggca acctgtacgg ttgcgccgcc ctaaaaacct
    11101 ttaccgaagc cgattgcggc gaaatcgcct gccttgccgt ctcgccgcag gcacaggacg
    11161 gcggctacgg cgaacgcctg cttgcccaca ttatcgataa ggcgcgcggc ataggcataa
    11221 gcaggctgtt cgcactgtcc acaaataccg gcgaatggtt tgccgaacgc ggctttcaga
    11281 cggcatcgga agacgagttg cccgaaacgc ggcgcaaaga ctaccgcagc aacggacgga
    11341 actcgcatat tctggtacgt cgcctgcacc gctgaccgca acggaaagcc gccgcagaaa
    11401 tgccgtctga accccgtttc agacggcatt tccccgatta taaagacctg cccaaccctc
    11461 aaggaccccg atgaaatcct accccgaccc ctaccgccat tttgaaaacc tcgattctgc
    11521 cgaaacgcaa aacttcgctg ctgaagcgaa tgccgaaacg cgcgcgcgtt ttttagaaaa
    11581 cgacaaggcg cgcgcgcttt cagacggcat tttggcgcag ttgcaggaca cgcggcagat
    11641 tccgttttgt caggaacacc gtgcgcggat gtaccatttc catcaggacg cggaatatcc
    11701 gaagggcgtg taccgcgtgt gcacggcggc gacgtatcgt tcgggctatc ccgagtggaa
    11761 aatcctgttt tcggtggcgg atttcgacga attgctcggt gacgatgtat atctaggcgg
    11821 cgtgtcgcac ctggtggaac agcccaaccg cgcgttgtta acactgagca aatcgggcgg
    11881 cgataccgcg tacacgctgg aagtggattt ggaagcaggg gagttggtag aaggcggttt
    11941 tcactttccg gcaggcaaaa accatgtgtc gtggcgcgat gaaaacagcg tgtgggtgtg
    12001 tccggcttgg gacgaacgcc agttgaccga atcgggctat ccgcgcgagg tgtggctggt
    12061 ggaacgcggc aagagtttcg aggaaagcct gcctgtgtat caaatcgata aaggtgcaat
    12121 gatggtgaac gcgtggcgtt atctcgatcc gcagggttcg ccgattgatt tgattgaagc
    12181 gtcggacggt ttttacacca agacgtattt gcaggtgtcg tctgaaggcg aggcgaaacc
    12241 gttaaacctg cccaacgatt gcgacgtggc cggctatctg gccggacatc ttttgctgac
    12301 attgcgtaaa gactggcacc gcgcgaacca aagttatccg agcggcgcgc tggtagcggt
    12361 gaaactgaat cgcggcgaaa tcggggcggc gcagcttttg tttgcgcccg atgaaacgca
    12421 ggcattggaa agcgtggaaa cgaccaagcg ttttgtcgtg gcgagcctgc tggaaaacgt
    12481 acaaggccgt ctgaaagcgt ggcggtttgc cgacggcaaa tggcaggaag tcgaactacc
    12541 gcgcctgcct tcgggcgcgt tggaaatgac cgaccaaccg tggggtggcg acgtggttta
    12601 ccttgccgcc ggcgatttca ccacgccgct ggcgctgttt gcattggatt tgaacgtgat
    12661 ggaactgacc gtcatgcgcc gtcagccgca gcagttcgat tcagacggca tcaacgtgcg
    12721 gcagttttgg gcggtttcgt ccgacggcga gcgcattcct tatttccacg tcggcaaaaa
    12781 cgccgcgccc gacacgccga cgctggtcta tgcctacggc ggtttcggcg tgcccgagct
    12841 gccgcattat ctgggcagca tcggcaaata ctggctggaa gagggcaatg cctttgtgtt
    12901 ggcgaacata cgcggcggcg gcgaattcgg cccgcgctgg catcaggcgg cgcagggcat
    12961 cagcaaacac aaaagcgtcg atgatttgct tgccgtcgtg cgcgatttgt ccgaacgcgg
    13021 catcagttcg cccgaacaca tcggtttgca gggcggcagc aacggcggac tgattaccgc
    13081 cgccgccttc gtgcgcgaac cgcaaagcat aggcgcgctg gtgtgcgaag ttccgttgac
    13141 cgacatgatc cgttatccgc tgctctccgc cggttcaagc tggacggacg aatacggcaa
    13201 tccgcaaaaa tacgaagtct gcaaacgccg gttgggcgaa ttgtcgccgt atcacaatct
    13261 ttcagacgac gtgccatacc cgcccgcgct tattaccacc agcctcagcg acgaccgcgt
    13321 ccatcccgcc cacgcgctca agttttacgc caaactgcgc gaaacctcgc cgcaatcttg
    13381 gctctactcg cctgacggcg gcggccatac cggcaacggc acgcagcgcg aagccgccga
    13441 cgaactcgcc tgcgtgttgc tgtttttgaa agagtttttg ggctaagggc ggggagcggc
    13501 actgccgccg cgaatgaaaa aaggtcgtct gaaactgctt tttcagacga ccttttttaa
    13561 tggttgtttc aaatcaaaat atctatgccg ccggccccat cagcacttct tcacatccga
    13621 aggcaaaaat ccgtaatgcc gtctgaacgc ttcgttgaac cgtcccgcgt ggcggtagcc
    13681 gcaaaagtgc atggcggctt ggacggtgct gccggattcg atgagggcga gcgcgtgttc
    13741 cagccgcagg cggcgcaggc atccggcgac ggtttcgccg gtttgcgctt tgaaatagcg
    13801 tttcaggtag cattcgttca gtccgacgcg gcgggcgatt tcggcgatgg tcagcggacg
    13861 ggcgaattcg tgttgcagga tgtcggcggc ttcgtctatg cgccggcggc ggtagccgtt
    13921 gtcgtggcgg cggaaggtga agcgcaataa tcgggcggag agttccagcg cggcggcttc
    13981 gtcggcaagc aggccgaagc cgtccgattc gaacgagcgt tgcagcagtg ggcaggccgc
    14041 cgccgtcagt gccgctgcgt tttgcgccag ccgttgcagg gtgaatcggc ctatcgtttg
    14101 cggcgaaaac aggcgttcgt ccagcaagcc ttcgtcgtgc cagcggcgca gtttttccag
    14161 cgaaaaatcc aaatgcagcg cgcacatgcc gctgttgtcg ggcaacaggg tttcggatac
    14221 gtccgccaac tcgccgcgta ccagccagat ttcgccggca gacgggcggt attccctgcc
    14281 gcccatttgt aaccggttct gccccgacac catgacgaac aaggcgcagt tgtggctgaa
    14341 attgtggatt tcggtgggaa acgcgcccgt tccgccgccg cgcatccgcg acaaggtgat
    14401 gcccgagtcg aagcggttga tgcacatttc cagatgcaaa ccgggctgtt ttgcctgcgc
    14461 aatgagcgcg ctgtcggaac agccgtccaa cgcccagccg gatttatcgg agcggacata
    14521 ggtttggtac tggcggtaga tggcggcggt gttcatgatt ggataggaac gagttgtcta
    14581 acaaatgaat taaataggaa tt
//
LOCUS       DAXHKX010000030        13988 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHKX010000030 DAXHKX010000000
VERSION     DAXHKX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.030"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(95..157)
                     /locus_tag="ACFMWZ_000760"
                     /pseudo
     CDS             complement(95..157)
                     /locus_tag="ACFMWZ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(263..1588)
                     /locus_tag="ACFMWZ_000761"
     CDS             complement(263..1588)
                     /locus_tag="ACFMWZ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC5934754.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSVQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1799..2638
                     /gene="efeU"
                     /locus_tag="ACFMWZ_000762"
     CDS             1799..2638
                     /gene="efeU"
                     /locus_tag="ACFMWZ_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC5934755.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2635..3801
                     /gene="efeO"
                     /locus_tag="ACFMWZ_000763"
     CDS             2635..3801
                     /gene="efeO"
                     /locus_tag="ACFMWZ_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC5934756.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            4065..5330
                     /gene="efeB"
                     /locus_tag="ACFMWZ_000764"
     CDS             4065..5330
                     /gene="efeB"
                     /locus_tag="ACFMWZ_000764"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC5934757.1"
                     /translation="MSKNQPAQPTKRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5402..5731)
                     /locus_tag="ACFMWZ_000765"
     CDS             complement(5402..5731)
                     /locus_tag="ACFMWZ_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC5934758.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5807..7177)
                     /gene="glmU"
                     /locus_tag="ACFMWZ_000766"
     CDS             complement(5807..7177)
                     /gene="glmU"
                     /locus_tag="ACFMWZ_000766"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC5934759.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDADTA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTGQAQELLKAGVTLHDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     ARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7232..7504)
                     /locus_tag="ACFMWZ_000767"
     CDS             complement(7232..7504)
                     /locus_tag="ACFMWZ_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC5934760.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7480..8211)
                     /locus_tag="ACFMWZ_000768"
     CDS             complement(7480..8211)
                     /locus_tag="ACFMWZ_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC5934761.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQEYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTKLHGMADTEHTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTPPRKYERMLCVKPSLS"
     gene            8270..9271
                     /locus_tag="ACFMWZ_000769"
     CDS             8270..9271
                     /locus_tag="ACFMWZ_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC5934762.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPGSAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9365..10213
                     /locus_tag="ACFMWZ_000770"
     CDS             9365..10213
                     /locus_tag="ACFMWZ_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC5934763.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10239..10724
                     /locus_tag="ACFMWZ_000771"
     CDS             10239..10724
                     /locus_tag="ACFMWZ_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC5934764.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10826..12394)
                     /gene="msrAB"
                     /locus_tag="ACFMWZ_000772"
     CDS             complement(10826..12394)
                     /gene="msrAB"
                     /locus_tag="ACFMWZ_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC5934765.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12539..13804
                     /gene="ftsY"
                     /locus_tag="ACFMWZ_000773"
     CDS             12539..13804
                     /gene="ftsY"
                     /locus_tag="ACFMWZ_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC5934766.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2991 a         3591 c         4186 g         3220 t
ORIGIN      
        1 agtacagccc caaatttaaa ccagtacggc gttggctcgc cttagctcaa agagaacgat
       61 tctctaaggt gctcaagcac caagtgaatc ggttccgtac tatttgtact gtctgcggct
      121 tcgtcgcctt gtcctgattt aaatttgatc cactataatt ccgtcaaata agaaaggaat
      181 tttgtgcctg cggtatcgca aaacttcgcc ttaatgcgcc cgattgccta gggatgggct
      241 tcagatggca ttgttttccg gtttacgggc ggtattcggg cttcataccg ttgggcagaa
      301 gctgccagac atatcccgtg gttttctgtt tgccggcaag ttcgccggct tcgtcgccgt
      361 atccccaaaa ataatccacg cgcaccgcgc ctttaatcgc gctgccggta tcctgcgcca
      421 taatcaggcg gttgagggct ttgcgggtaa ccggatgggc ggtggcgaca aataagggcg
      481 cgcccaaggt aatgtagtgc cggtcgactg cgccggcgta ctcgcccatc agcggcgtgc
      541 ccagtgcgcc gacagggccg tcattgctgc ttccggtaag ctctcggaaa aagatatagc
      601 tggggttttg ccccaaaact tcggcgaggc gttgcgggtt ttgctgcata taggctttga
      661 tgccctgcat cgaggtctgc ccgagcttga ggtagccttt gtccgccata tagcgtccga
      721 tggaaacgta gggatgttcg tttttgtcgg catagccgat gcggatgtat ttgccggacg
      781 gggttttcag acggcccgag ccttggatgt gcataaaaaa aagttcgacg gggtcttcgg
      841 cgtaaccgag tatcggggct ttgccgtcaa gcgcgccgcc gttgatttgg ttgcgcgtgt
      901 ggtaggggag gaagcggctt ccttcaaacc tgcctttgat tgccgttgtg cgcgcagtga
      961 tggggaattg ggagaggtcg gcggtatgtg tgccgccggt attgtcgatt gtgccgctgt
     1021 tttttcccgt ctgcctgatg cggacaaggg cttttccgct ccgcaaaccg gcaggcaggg
     1081 ggacggagat aaaatcgtcg ggaataccgt aaatcgggaa gcgggcttgt gccgtccgcc
     1141 tgtcgtcgcc cttcagcacc ggctcgtaat agccggtaac cgtaccggca aggcttccgt
     1201 tgcctgcaac ctgccacggc gtgaaatagc gttcaaaaaa ctgttttgcc tgaacggaat
     1261 ggacgggggt ttgaaaggct tgggcgcaca catcctgcca gccttggcgg tttttcaaat
     1321 tggcgcagcc gaggcggaag gattgcaggc ttttggcgaa atcctgcgcc gcccagtggg
     1381 gcagggacag gtgcggcaca acggtataaa cggccccgcc gccgccgacc gtcgttccgg
     1441 cggggtcggg gatgccgacc ggccggtccg ggccgttgat gacggatgtg tcgggttgcg
     1501 gaaaggtttg gatgctcttg ctttggcagg cggcgaggat ggcggcggcg atgccgtaca
     1561 gggcggcgcg gaataggtat tttttcataa tggacaatgt tgccggcagt aataagaaag
     1621 atggtttcgg gcggcgttgc ggcagccgtg gagaggggat tttaacacag ggcgcagctg
     1681 cagcctgcgg aactttccgc cgcgcggcaa tgcagataaa aataacttgc atttgtattt
     1741 acaaacaatg aaaatattcc gataatatat tattcatcat ccttgttcgt tcgcgtttat
     1801 gctggttgct tttttaatta tgttgcgcga gggtatcgaa gctgcactga ttgtcggtat
     1861 cgttgcgggt tttttgaaac agtccggaca ttccaaactg atgcctaagg tctggttcgg
     1921 ggttgtcctt gcttctttga tgtgtttggg tatcggatac ggcatccatt cggcaacggg
     1981 cgagattccc cagaagcagc aggagttcgt cgtcggtatt atcggtttgg ttgccgtcgc
     2041 gatgctgact tatatgattt tatggatgaa aaaggcggcg cgttcgatga agcagcagct
     2101 tcaggactcc gtgcaggcgg ccttgaaccg tggcagcggt caaggatggg ccttggtcgg
     2161 tatggcgttt cttgctgtgg cgcgcgaagg tctggagagc gtttttttcc tgcttgccgt
     2221 attcaaacag agcccgacgt ggcagatgcc ggccggtgcg gtagcggggg ttttggctgc
     2281 cgccgtgatt ggcgcgttga tttatcaggg tgggatgcgc ctgaatctgg cgaagttttt
     2341 ccgttggacg ggggcgtttt tgattgtcgt tgccgccggc ctgcttgccg gctcgctgcg
     2401 cgcgctgcat gaggcaggta tttggaacgc gcttcaggac attgtgttcg actcatcaaa
     2461 atatttgcac gaagacagcc cgttgggcgt gctgctcggc ggatttttcg gctataccga
     2521 ccatccgacg cagggcgagg ccttggtttg gctgctgtac cttattcccg tcataacttg
     2581 gtttttgtgc ggcagcaggc cgtctgaaac tttaacccgt aaagaggagc tgaaatgaga
     2641 aaattcaatt tgaccgcatt gtccgtgatg cttgccttag gtttgaccgc gtgccagccg
     2701 ccggaggcgg agaaagctgc gccggcagcg tccggtgagg cgcaaaccgc caacgagggc
     2761 ggttcggtca gtatcgccgt caacgacaat gcctgcgaac cgatggaact gaccgtgccg
     2821 agcggacagg ttgtgttcaa tattaaaaac aacagcggcc gcaagctcga atgggaaatc
     2881 ctgaaaggcg tgatggtggt ggacgagcgc gaaaacatcg cccccggact ttccgataaa
     2941 atgaccgtca ccctgttgcc gggcgaatac gaaatgactt gcggtctttt gaccaatccg
     3001 cgcggcaagc tggtggtaac cgacagcggc tttaaagaca ccgccaacga agcggatttg
     3061 gaaaaactgt cccaaccgct cgccgactat aaagcctacg ttcaaggcga ggttaaagag
     3121 ctggtggcga aaaccaaaac ctttaccgaa gccgtcaaag caggcgacat tgaaaaggcg
     3181 aaatccctgt ttgccgacac ccgcgtccat tacgaacgca tcgaaccgat tgccgagctt
     3241 ttcagcgaac tcgaccccgt catcgatgcg cgtgaagacg acttcaaaga cggcgcgaaa
     3301 gatgccggat ttaccggctt ccaccgtatc gaatacgccc tttgggtgga aaaagacgtg
     3361 tccggcgtga aggaaattgc agcgaaactg atgaccgatg tcgaagccct gcaaaaagaa
     3421 atcgacgcat tggcgtttcc tccgggcaag gtggtcggtg gtgcatccga actgattgaa
     3481 gaagtggcgg gcagtaaaat cagcggcgaa gaagaccggt acagccacac tgatttgagc
     3541 gacttccaag ccaatgtgga cggttcgaaa aaaatcgtcg atttgttccg tccgttgatc
     3601 gagaccaaaa acaaagcctt gttggaaaaa accgatacca acttcaaaca ggtcaacgaa
     3661 attctggcga aataccggac taaagacggt tttgaaacct acgacaagct gggcgaagcc
     3721 gaccgcaaag cgttacaggc ctctattaac gcgcttgccg aagaccttgc ccaacttcgc
     3781 ggcatactcg gcttgaaata agccgcaagc gttcagacgg tatttggcgg cagataccgt
     3841 ctgaagtttt atagtggatt aacaaaaacc agtacggcat tgcctcgcct tgccttgccg
     3901 tactatttat actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
     3961 ccgccatata ttgcagggcg ggatttcaac ctgccgctat cggttaatgg aaaaacggcg
     4021 tgcagggata cccatcctgc tgcacggata ttgaaggaaa caccatgagc aaaaaccaac
     4081 ccgcacaacc gaccaagcgc actcttttta aaaccgcgat cgcagccgga gcagtcggcg
     4141 caatcggagg ttatctcggc ggcaaaaaac ggggcgaaac cgccgaacgc accgccgaaa
     4201 gccaacactc gccccaagcc tatccctgct acggcgaaca tcaggcaggc atcgttacgc
     4261 cgcagcaggc gttttcgatt atgtgcgcct tcgacgtaac cgcgcaaagt gccaagcagc
     4321 tggaaaacct gttccgcacg ctgaccgccc gcatcgagtt tctcacccaa ggcggcgaat
     4381 accaagacgg cgacgacaaa cttccgccag ccggcagcgg cattttgggc aaagccttca
     4441 accccgacgg gttgaccgtt accgtggggg tgggcagcag cctgtttgac ggccggttcg
     4501 gactcaaaga caaaaaaccg attcatttgc aggaaatgcg cgacttctcc aacgataagc
     4561 tgcaaaaaag ctggtgcgac ggcgatttga gcctgcaaat ctgtgccttc acccccgaaa
     4621 cctgccaagc cgccctgcgc gacatcatca aacacaccgt ccaaaccgcc gttatccgct
     4681 ggagtatcga cgggtggcag cctaaatccg aacccggcgc gatggcggcg cgcaacctgt
     4741 tgggcttcag ggacggcacg ggcaacccca aagtttccga ccccaaaacc gccgacgagg
     4801 ttttgtggac gggtgtggcc gccaacagcc tcgacgaacc ggagtgggcg aaaaacggca
     4861 gctatcaggc agtccgcctt atccgccact ttgtcgagtt ttgggacagg acgccgcttc
     4921 aagagcaaac cgacattttc gggcggcgca aatacagcgg tgcgccgatg gacggcaaaa
     4981 aagaagccga ccaaccggat tttgccaaag accccgaggg gaataccacg cccaaagaca
     5041 gccatatacg cctggcgaat ccgcgcgatc ccgaattcct caaaaaacac cgcctcttcc
     5101 gccgcgccta cagctattcg cgcggactcg cctcaagcgg acagcttgat gtcgggctgg
     5161 tgttcgtctg ctatcaggca aaccttgccg acggattcat cttcgtgcaa aacctcctca
     5221 acggcgaacc gctggaagaa tacatcagcc ccttcggcgg cggctatttc ttcgtcttgc
     5281 ccggcgtgga aaaaggcggc tttttggggc aagggctgct gggcgtataa atccgccata
     5341 taaaaaacgc cgtccgaacc ttgccaaacg ggttcggacg gcgtttcttg tttttgggcg
     5401 gtcaggcttt tttgacgaat tcggatttta aattcatcgc gctgccgtcg attttgcagc
     5461 cgatgttgtg atcgccttct tgcaggcgta tgcctttgac ttttgtacct tgtttgatcg
     5521 tcatcgagct gccttttacc ttgaggtctt tgatgaggat gacggtgtcg ccgttttgca
     5581 gcactgcgcc gttggcatcg agcacttgag ccgcaaggtc ggcggcggat tcggtttcat
     5641 tccattcgtg ggcgcattcg gggcagatgt attgtccgcc gtcttcatag gtgtattcgg
     5701 aggcacattg cgggcatggg ggtaatgaca tggttttccg tccttatcgg atgtttgttt
     5761 ttgggatgcc gtctgaaacc tgaaaccggc ttcagacggc atggcgttat tgtttgtctt
     5821 tttcgggacg cacccagcct tcgatgacgg tttggcgggc gcgggcgagg gcgagtttgt
     5881 tgtcttcgat attgcgggta atcgtgctgc ctgcgcctgt ggttactttg ttgccgattt
     5941 tgactgggga gaccaagacg cagtttgaac cgatgcggac ttcgtcgccg atgacggttt
     6001 tgtgtttgtg cacgccgtcg tagttggcaa taatcgtgcc tgcgccgaag ttggttttgc
     6061 tgccgacttc ggcatcacca atgtaggtga ggtggttggc tttggtgcct ttgccgatgg
     6121 cggcgttttt gatttcgacg aagttgccga cgtgtacgtc gtctgcaagg cgggcttgcg
     6181 gacgcaggcg ggcgtacggg ccgattcggt tgttttcgcc gacttcgcag tcttcgaggt
     6241 gggagaaggg ggcgattttg ctgtttgcac cgattttggc gtttttgatg acgcagtttg
     6301 cgccgatttc gacgttgtcg ccgatttcga cttcgccttc aaagatacag ttcacatcaa
     6361 ttacgacatc ctgtccgtgt ttcagacggc ctcgtaaatc gaaacgtgcc gggtcgtgca
     6421 gggtaacgcc tgctttgagc aattcttgcg cctgtccggt ttggaagatg cgttcgagtt
     6481 cggcgagctg gcgtttgttg ttcacgccgg cggcgaggtg ggaggcgcgg acttggacgg
     6541 gatgaacttt aataccgtcg gcaacggctt tggcgatgag gtcggtcagg tagtattcgc
     6601 cttgggcatt gttgctggaa aggctgttca gccagttttc cagtttggcg ttgggcagga
     6661 cgaggatgcc ggtgttggtt tctttcacgg ctttttgggc ggtatcggcg tctttttctt
     6721 cgacaatggc ggttacgctg ccgctgccgt cgcggatgat gcgccccaaa cctgacgggt
     6781 cggcgggcac gtcggtcaac agcccgactt cgttgcctgc ggcttcgagc agggtttcga
     6841 gggtttcaac gtcaattaaa ggaacgtcgc cgtacaacac cagcgtgcgg ccttcggcgg
     6901 caaggtgggg cagggcggtt ttgacggcgt ggccggtgcc gagctgttcg gtttgttcaa
     6961 cccagacgac atcgcgtttg acggtgtcta aaacttgctc tttgccgtgg ccgacgacga
     7021 cgcagatgtt ttgcggattc agcgcggcgg cggtgtcgat aacgcgcccg agcattgttt
     7081 cgccgccgat ttcgtgcaaa accttgggca ttttggaata catgcgcgtg cctttgccgg
     7141 cggcgaggat gacgatgttt aaagtgtttt gcggcatagc gttttcctgt gcaatgccgt
     7201 ctgaaagggc ttcagacggc atagggtggg tttatcggtt ttgaaatttg ggcttttgcc
     7261 agtgttggcg atgctcttcg tcggcgttgt tgccggtttg gttgggtaac acggcatggc
     7321 gttcgggacg gtattggttg tagttcatat ttttcgagta gctgccgtct tggtaataaa
     7381 cgggcgtgcc ggcgggatat ttttgacgga cggcggtctt gccgttgccg tcttgataag
     7441 tttcccacgc gcagcccgac aaaagggcgg cggcagtcat caggataagg aaggttttac
     7501 gcataacatt ctttcgtatt ttcggggggg tgtgcgggta ttgtaatgat tttggcggtg
     7561 ttctgacaaa gtttctgcat accgagccag ttgcgccata tcgcttacgg aagcatcgat
     7621 aaagggcagg tcgtgcactt ttgcaccgaa ccggacggtt ttcatgccca atgcttttgc
     7681 ctgatgcagg ttgtccgcgc tgtcgtccac cataatgcag cattcgggcg gtacgtccaa
     7741 aaggcggcag acattgagat acgcttgcgg attgggtttg tacagcagtc cgaaatcatc
     7801 cgtgccgaac agtgcgtcga aacggttttc caaaccgagt gcgccgacaa cggcacggac
     7861 gtaaaacgac ggaccgttgg aaaaaaccgc cttgcgcccg ttcagacggc atagggtgtg
     7921 ttccgtatcc gccatgccgt gcagcttggt caggattgca tcgatcggat ggctttcgcg
     7981 taaaaattcg gcgatgtcga tttcgggatg gtggatttgc agtccggcga gcgttgcgcc
     8041 gtagcggtgc caatattcct gacgcaggtc ggacgcggcg gattcggaga gtttgaggcg
     8101 gcgtgccata tagcgtgtca tagcgcggtt gatgagtgtg aagatgcctg cgtcggcatc
     8161 gtgcagcgtg ttgtcgaggt cgaacagcca cacggtcggg ttttcttgca ttttgaaccg
     8221 tgaaaatttg ttagaatgtt attttacagc gaatagagga ggactcggaa tgaaacggaa
     8281 aatttggctg ctgccgctgc tggcggtttc ggcatacctg caggcgcaga cggaagtcag
     8341 gctggcggtg cataagtcgt tcagcctgcc caaagggctg attgcgcgct tcgagcgggc
     8401 aaacgatgcg aaggtgtcga ttattcaggc gggcggcgcg aacgaaatgc tcaacaaact
     8461 gattttgagc cgcgccaacc cgatcgccga cgcggtgtac ggcttggaca acgccaatat
     8521 cggcaaggcg cgggaaatgg gcattttggc ggcggcgcaa cccggatccg cccccgtcgc
     8581 ggtcgggctg ccttcggctt tggcggtcga ttacggctat gtgtccatca attacgacaa
     8641 aaaatggttt gaaggcaaaa agctgcccct gccgcaaacc ctgcaggatt tgacccgccc
     8701 cgaatataaa aacctattgg tcgtaccgtc tcccgccacg tcgtccccgg ggctgggctt
     8761 cctgatggcg aacatcagcg gtctgggcga agaaggcgcg ttcaaatggt gggcacagat
     8821 gcggcagaac ggcgtgaagg tcgccaaagg ctggagcgag gcgtattaca ccgacttttc
     8881 gcacaacggc ggcgcgtatc cgctggtggt cggttatgcc gccagtccgg cggcggaagt
     8941 gtatttttcc aaaggcaaat acagcgagcc gccgacgggc aacctgtttt taaaaggcgg
     9001 cgtattccgc caggtcgaag gcgcggcagt cttgaagggc gcgaaacagc cgaagctggc
     9061 ggcaaaactg gtgcaatggc tgcaaagtcg ggaagtgcag caggcggttc cgtccgaaat
     9121 gtgggtttac cccgccgtca aaaacacgcg cctgcccgac gtgttccgct tcgcccaagc
     9181 cccgacgcac accaccgccc ccgcgcagcg cgatattgat gcgaaccagc gcggatgggt
     9241 ttcccgttgg attagaacgg ttttgaaata aaacaaacat accgccccgc agggcttcat
     9301 acggcatttt tacacctgtg ccgattacgc cgcatggggc ggatgttcga tcaagaggaa
     9361 aacaatggac ttcaaacaat ttgatttttt acacctgatc agtgtttccg gttgggagca
     9421 tctggctgaa aaggcgtggg cgttcgggct gaaccttgcc gccgcgctgc ttattttttt
     9481 ggtcggaaaa tgggcggcga aacgcattgt cgccgtgatg agggcggcga tgacgcgcgc
     9541 gcaggtcgat gccacgctga ttagtttttt gtgtaatgtt gccaatatcg gcttattgat
     9601 tttggtgatt attgccgcat tgggcagatt gggcgtttcc acaacatccg taaccgcctt
     9661 aatcggcggc gcgggtttgg cggtggcgtt gtccttgaaa gaccagctgt ccaattttgc
     9721 cgccggcgcg ctgattatcc tgttccgccc gttcaaagtc ggcgatttta tccgcgtcgg
     9781 cggttttgaa ggatatgtcc gagagattaa aatggtgcag acttctttgc ggacgaccga
     9841 caacgaagaa gtcgtgctgc ccaacagcgt ggtgatgggc aacagcatcg tcaaccgttc
     9901 caccctgccg ctgtgccgcg cccaagtgat agtcggcgtc gattacaact gcgatttgaa
     9961 agtggcgaaa gaggcggtgt tgaaagccgc cgtcgaacac cccttgagcg ttcaaaacga
    10021 agagcggcag gccgccgcct acatcaccgc cttgggcgac aatgccatcg aaatcacatt
    10081 atgggcttgg gcaaacgaag cagaccgctg gacgctgcaa tgcgacttga acgaacaagt
    10141 ggtcgaaaac ctccgcaaag tcaatatcaa catcccgttc ccgcaacgcg acatacacat
    10201 catcaattct taaacgccgt ctgaaagagg agtgggaaat ggacgcgctg cacaccatcg
    10261 cccgaaacct gacgaaaaaa cgtcaaaccg taagctgtgc cgaatcctgc acgggtgggc
    10321 tgcttgccgc cgtattcaca agcgttgcag gcagttcgca atggttcgat caaagttttg
    10381 taacatacag caacaaagcc aaagaagacc gcttgggcgt gttgcccgaa accctgctcg
    10441 aacacggcgc ggtcagccgc caaaccgtct atgagatggc gcgcggcgcg aaagccgtgg
    10501 cgcaggcgga ttacgccgtc ggtatttccg gcatcgccgg tccgggcggc ggcagcgaaa
    10561 gcaaacccgt cggcacggtt tggttcgggt ttgcctttcc gggcggaagt tgcgaagcaa
    10621 tgcgccgttt tgacggcaac cgcgaatccg tccgcgcgca ggcggtcgcc ttcgcgttgg
    10681 aacggttggc ggggctgatt gaaaacggcg gcgatgctgt ctaaacaaaa tctccgtctg
    10741 gacagaatcc ccgccggata aaaaataccg tctgaaacgt tgcaggtttc agacggtatt
    10801 ttgtcgggtt aggcggcggt gcggcttatt tcactttact cttcaacgcg ccgtagcctg
    10861 ccgcgtccat ttgttccagc gggatgaatt tcaagctcgc gccgttgatg cagtagcgca
    10921 gtccgccttt gtcgcgcggg ccgtcgggga agacgtgtcc caaatgcgaa tcggcggcgt
    10981 ggctgcgcac ttcggtgcgg cgcatgttgt agctgaaatc atcgtgttcg gtaacggatt
    11041 ttgcatcaat cgggcgcgtg aagctcggcc agccgcagcc ggaatcatat ttgtcggcgg
    11101 agctgaacaa aggttcgccg ctgacaacgt ccacataaat gccgggtttg aacaaatggt
    11161 cgtattcgtg gctgaaggca tattcggtcg cgctgttttg ggtaacttgg tattgctctt
    11221 cggtcagggt gcgtttgagt tcggcgtcac tcggtttttt atacgttgcc gcatcgaagc
    11281 ctttgccttg cggggcggtc ttggttttgc ccggcagcgg ttcgtcggct ttgcggatat
    11341 cgatgtggca gtagccgttg gggtttttaa tcaggtagtc ctgatggtat tcctcggcat
    11401 cgtagaagtt tttcagcggc tcgttttcaa caacgagggg cagttggtat ttttgctgct
    11461 cgcgtttgag ggcggcggcg atgacggctt tttcggcggg gtcggtgtag tacacgccgc
    11521 tgcggtattg cgtaccggtg tcgttgccct gtttgttgag gctggtcgga tcaacgacgc
    11581 ggaagaaata ttgcaggatg tcgtctaggc tgagtttgtc ggcatcgtag gtcactttga
    11641 cggtttcggc gtggcccgta tggcggtagg acacgtcttc atagctcgga tttttcgtgt
    11701 tgccgttggc gtagccggat accgcgtcaa ccacgccgtc gatgcgttgg aaataggctt
    11761 ccaagcccca gaagcagccg ccggcgaggt aaatggtgcg cgtgttcatg atttttgaat
    11821 cctttttctg agtgtcgggt ttgtagaacg aatgtttcaa gctgcccaaa tcggcattcg
    11881 ggtcgcggat taacgccaac gcctgcgctt cgttgatgct gcctttgacg atgcgctgca
    11941 cgtcgccgtc tttaccgatt aacgcccacg aagggtaaac gctgatattc aggctttggg
    12001 cgatcgtgcc gccgttgtcg gttacgacgg gcagcttggg ataattcaaa ccggcatacc
    12061 atttttggaa gtcgccgtct tttttctcgt gcaaaaagcc cggagacgcg acggtaatca
    12121 ggttggcgga gctgaatttt gcatcttgcg cccatttttc ggtctgtccc aattcggaca
    12181 gacacaaagg acaccagctc gcccaaaatt taatcagcgt cggtttgtct tttttcaagt
    12241 aaacactggc ggggcggttg tccgcggttt tcaaagtgga taaagtgtgc ggcacggtcg
    12301 cggctccggc atcgacgatt ttgggcgaac aagcacccag cgcaagcagg cagccgaact
    12361 tggcgcaaag ggaaaagaaa gtacggtgtt tcattttgag tttcctgtgt ggacggtttg
    12421 catgattaga cgtttgagat gccgaaacct tacagctcgg attttcagac aaccttgccg
    12481 cgtaaaatac gctacaatac gccctgtttc aagtttctaa aattaaaagg aaaattcaat
    12541 gttcagcttc ttccgtcgca agaaaaaaca ggaaacgccg gctctcgagg aggctcaaat
    12601 tcaggaaacc gcagcaaaag cagagtccga aaccgatcaa gtaattgaaa atattaaaga
    12661 agatgctgaa tctttagcag aaagcgtcaa agggcaggtc gaatctgccg ttgaaaccgt
    12721 cagcggtgcg gttgaacaag taaaggaagc cgttgccgag atgctgtctg aagcagagga
    12781 agcggcggaa aaagcagcgg aacaagtcga agcggcaaaa gaagccattg ccgaaaccgt
    12841 cggcgaggct gtcgggcaag ttcaagaagc cgttgcaaca actgaagaac acaagctcgg
    12901 ttgggcggcg cgtttgaaac aaggtttgac caaatcgcgc gacaaaatgg cgaaatcgct
    12961 ggcgggcgtg ttcggcggcg gacaaatcga cgaagatttg tacgaagagc tggaaaccgt
    13021 gctgattacc agcgacatgg gcatggaagc caccgaatac ctgatgaaag acgtgcgcga
    13081 ccgcgtcagc ctcaaagggc tgaaagacgg caacgaattg cgcggcgcgt tgaaagaagc
    13141 cttgtacgac ctgattaagc ctctggaaaa accattggtc ttgcccgaaa ccaaagagcc
    13201 gtttgtcatc atgcttgccg gcatcaacgg cgcgggcaaa accacgtcta tcggcaaact
    13261 cgccaaatat ttccaagcgc agggcaaatc cgtattgctg gcggcaggcg acactttccg
    13321 cgccgccgcc cgtgagcagc ttcaggcatg gggcgaacgc aacaacgtta ccgtgatttc
    13381 gcaaaccacg ggcgattccg ccgccgtgtg cttcgatgcc gtccaagccg ccaaagcgcg
    13441 cggcatcgac attgtgctgg ccgacaccgc cggccgcctg cccacgcagc ttcatttgat
    13501 ggaagaaatc aaaaaagtga aacgcgtgct gcaaaaagcc atgcccgacg cgccgcacga
    13561 aatcatcgtc gtgcttgatg ccaacatcgg gcaaaacgcc gtcaaccaag tcaaagcctt
    13621 tgacgacgca ttagggctga ccggtttaat cgtgaccaaa ctcgacggca cggcaaaagg
    13681 cggcatcctc gccgcgcttg cctccgaccg ccccgtcccc gtccgctaca tcggcgtggg
    13741 cgaaggcata gacgatttgc gcccgtttga cgcgcgcgcg tttgtggacg cactgctgga
    13801 ttgagccgaa atgccgtccg aaaacagcag accgatgccg tcattcccgc gcaggcggga
    13861 atccagacct tgggataaca gcaatattca aagtttataa aagacctgtc attcccgcgc
    13921 aggcgggaat ctagaaccat cggcacggaa acttatcggg taaacggttt cttgagattc
    13981 cgcgtcct
//
LOCUS       DAXHKX010000031        13906 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHKX010000031 DAXHKX010000000
VERSION     DAXHKX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.031"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(91..864)
                     /locus_tag="ACFMWZ_000774"
     CDS             complement(91..864)
                     /locus_tag="ACFMWZ_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC5934767.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQPATVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
     gene            complement(963..1586)
                     /gene="rsmG"
                     /locus_tag="ACFMWZ_000775"
     CDS             complement(963..1586)
                     /gene="rsmG"
                     /locus_tag="ACFMWZ_000775"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC5934768.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYMDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTGHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(1682..2167)
                     /locus_tag="ACFMWZ_000776"
     CDS             complement(1682..2167)
                     /locus_tag="ACFMWZ_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934769.1"
                     /translation="MFVNEKYPYAALFAGLVFLTLPFALAVHDAFALAFGRTGLLVSV
                     SDGGFGWRGGWDGVVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYTLFLSGVAAC
                     AAAAVAWIFELLLGSGALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            2273..2348
                     /locus_tag="ACFMWZ_000777"
     tRNA            2273..2348
                     /locus_tag="ACFMWZ_000777"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2306..2308,aa:Thr,seq:cgt)
     gene            2418..3545
                     /locus_tag="ACFMWZ_000778"
     CDS             2418..3545
                     /locus_tag="ACFMWZ_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC5934770.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRFGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            3725..4282
                     /gene="frr"
                     /locus_tag="ACFMWZ_000779"
     CDS             3725..4282
                     /gene="frr"
                     /locus_tag="ACFMWZ_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC5934771.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAVKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            4338..5084
                     /locus_tag="ACFMWZ_000780"
     CDS             4338..5084
                     /locus_tag="ACFMWZ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC5934772.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFSRQILKGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAKGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            5087..5884
                     /locus_tag="ACFMWZ_000781"
     CDS             5087..5884
                     /locus_tag="ACFMWZ_000781"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC5934773.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            5889..7124
                     /gene="ispC"
                     /locus_tag="ACFMWZ_000782"
     CDS             5889..7124
                     /gene="ispC"
                     /locus_tag="ACFMWZ_000782"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC5934774.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            7159..8499
                     /gene="rseP"
                     /locus_tag="ACFMWZ_000783"
     CDS             7159..8499
                     /gene="rseP"
                     /locus_tag="ACFMWZ_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC5934775.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            8556..10949
                     /gene="bamA"
                     /locus_tag="ACFMWZ_000784"
     CDS             8556..10949
                     /gene="bamA"
                     /locus_tag="ACFMWZ_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC5934776.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            11015..11515
                     /locus_tag="ACFMWZ_000785"
     CDS             11015..11515
                     /locus_tag="ACFMWZ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC5934777.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKVQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            11548..12591
                     /gene="lpxD"
                     /locus_tag="ACFMWZ_000786"
     CDS             11548..12591
                     /gene="lpxD"
                     /locus_tag="ACFMWZ_000786"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC5934778.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKAHGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVMHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVHIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            12627..13076
                     /gene="fabZ"
                     /locus_tag="ACFMWZ_000787"
     CDS             12627..13076
                     /gene="fabZ"
                     /locus_tag="ACFMWZ_000787"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC5934779.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFESMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            13283..>13906
                     /locus_tag="ACFMWZ_000788"
     CDS             13283..>13906
                     /locus_tag="ACFMWZ_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC5934780.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASV"
BASE COUNT         3452 a         4295 c         3479 g         2680 t
ORIGIN      
        1 atgacgatat aagtattttt attttaatcc gccatattaa cgcacccggc caaacagcat
       61 aaaggcacgg gcagcccgat ttggattgac ctatttcccc gacaccctcg ccatcagctc
      121 gtccgccaag gcaagatacg ccttggtacc ctttgcctgc gcgtcgtaag ccatcaccgg
      181 cataccgtgg ctcggcgctt ccgcaaggcg gatattgcgc gggatggcgg tttcaaaaag
      241 caaatccccg aaatggctgc gcaactgttc gctgacttcg gcaaccagcc tgctgcggct
      301 gtcgtacatc gtgcgcacga tgcccgtgat gtccaaatcg ggattgaccg cctgacggat
      361 tttgcgcacg gttgcaatca aatcggaaat cccttccagc gcgtaatatt cgcacaacat
      421 cggcacaatc acgccgcccg ccgccaccaa gccgttaagc gtcaacagcg tcagcgaagg
      481 cggacagtcg atcaggataa agtcgtaatc ttccgccact gccttgagtg cgtttttcaa
      541 acgcacttcc cgggcgattt cctgcaccag ctcgatttcc gcaccggcca gcgcgcggtt
      601 cgcacccaac acagcgtatc cgccctcttt gctgcgtacc gtcgccggct gcacgtccgc
      661 atcgcccaat aagacctgat aaacgccgga ctgcaaaccc gccttgtcga tgccgctgcc
      721 cgtcgtcgca ttgccctgcg gatccaaatc gaccaccagc acgcgtttgc cgcgcgatgc
      781 cagcgaagcc gccaaattta ccgtcgtcgt cgttttgccc acaccgccct tctgattggc
      841 gatggcaagg atgttcgcac tcatgtcccg cccgtgtccc gtctgatgtt gaaaaaagaa
      901 aaacagtgcg gaattttacc tttatccgca caaaaaaggt attcatgccg tctgaagtgc
      961 gctcaacgct tgctcaggat gacgatatgg cgttccgcat ccaagcctgg cacgtcgagc
     1021 ctttggactt tttcaacgca cacatcctgc ggcaggcggc cgatttcccc ctgcggatac
     1081 acgcccttca tcgccgccca gtatccgccg tctttcaaca gatgccccgt ccacgacaca
     1141 aagtccgcca gttctgcaaa cgcacggctg gtaaccacat cggcacgcac gtccgaaacc
     1201 gcctccacgc gtccggatac cacgcgcaca ttgtccaacc ccaactcgat aaccgcctgc
     1261 tgtaaaaaag ccgttttctt cgtatttgca tccaaaaggg ttatctgcac atccggacgg
     1321 cacaccgccg ccggaatgcc gggctgaccg ccgcccgaac cgacatccag catcgtttgc
     1381 acaccctcga tatggggcag cagcgtcagg ctgtccaaaa gatgatggac aatcattttt
     1441 tcctcgtcgc gcagggcggt cagattgtag gttttgttcc actttttcaa caaatccata
     1501 taggccaaaa gcctgtcctg cgccgctgcg gaaatatcca aacccaatgc ggcgatgcct
     1561 gcacgcaggc gttctttgcg ttccatatcc ctatcctgtc catgttcagg cgtaatgtta
     1621 acggaaaaac atgccgtctg aaaccgcgtg tcggggttca gacggcattt cccgttccaa
     1681 cctatttttt cctgtgaaac accaccggct gcaccggcgc gcgcgtcagg cgtttgggca
     1741 gcgtcagcat cgccaccagc cacacggcaa acgcatattc caacaccgcc tccccacgca
     1801 gcccgcccaa agccccactg ccaagcagca gctcgaaaat ccaagccacc gctgccgccg
     1861 cgcaagccgc cacgcccgac agaaacagtg tgtaacgcga atgccgccgc atcatctttt
     1921 tgtacgccag tttcgtcaga cccgccgaca caaccacatt caaaaacgca aacacaccga
     1981 acacaaacca caccacgccg tcccagcctc ctcgccagcc gaatccgccg tccgacaccg
     2041 acaccagcaa ccccgtccgt ccgaacgcaa gcgcaaaggc atcatgcacc gccaacgcaa
     2101 acggcagcgt caaaaacacc agtcccgcaa acagagccgc ataaggatat ttctcgttca
     2161 caaacatcgc cgtccccctc ttccgaagca gaccgcatta taccgcgcac tgccttgccg
     2221 cccgccagaa aagttgcaca aacaaccgtt catatatatc ataacgaaaa acgccggtgt
     2281 agctcagtcg gtagagcagc gcattcgtaa cgcgaaggtc gggggttcga ttcccttctc
     2341 cggcaccaat accaagcaca gaccctccct cgggaagcct gtgctttttc acatttccgc
     2401 ttcagacggc aaccgatatg aacacctcgc aacgcaaccg cctcgtcagc cgctggctca
     2461 actcctacga acgctaccgc taccgccgcc tcatccacgc cgtgcggttc ggcggagccg
     2521 tcctgttcgc caccgcctcc gcccggctgc tccacctcca acacggcgag tggataggga
     2581 tgaccgtctt cgtcgtcctc ggcatgctcc agtttcaagg ggcgatttac tccaaggcgg
     2641 tggaacgtat gctcggcacg gtcatcgggc tgggcgcggg tttgggcgtt ttatggctga
     2701 accagcatta tttccacggc aacctcctct tctacctcac cgtcggcacg gcaagcgcac
     2761 tggccggctg ggcggcggtc ggcaaaaacg gctacgtccc tatgctggcg gggctgacga
     2821 tgtgcatgct catcggcgac aacggcagcg aatggttcga cagcggcctg atgcgcgcga
     2881 tgaacgtcct catcggcgcg gccatcgcca tcgccgccgc caaactgctg ccgctgaaat
     2941 ccacactgat gtggcgtttc atgcttgccg acaacctgac cgactgcagc aaaatgattg
     3001 ccgaaatcag caacggcagg cgcatgaccc gcgaacgcct cgaagagaac atggcgaaaa
     3061 tgcgccaaat caacgcacgc atggtcaaaa gccgcagcca cctcgccgcc acatcgggcg
     3121 aaagccgcat cagccccgcc atgatggaag ccatgcagca cgcccaccgt aaaattgtca
     3181 acaccaccga gctgctcctg accactgccg ccaagctgca atctcccaaa ctcaacggca
     3241 gcgaaatccg gctgctcgac cgccacttca cactgctcca aaccgacctg caacaaaccg
     3301 tcgcccttat caacggcaga cacgcccgcc gcatccgcat cgacaccgct atcaaccccg
     3361 aactggaagc cctcgccgaa cacctccact accaatggca gggcttcctc tggctcagca
     3421 ccaatatgcg tcaggaaatt tccgccctcg tcatcctgct gcaacgcacc cgccgcaaat
     3481 ggctggatgc ccacgaacgc caacacctgc gccaaagcct gcttgaaaca cgggaacacg
     3541 gctgacggtc ggacacgatg ccgtctgaaa cgcctgtcgc ttcagacggc atccccaatc
     3601 cgcctacctt gtcgttatgc ccgaataacg gtactattcg cggttaacgg tctttatgcc
     3661 gtctgaacgg cttgaaacgc gttcagacgg tctcccgccc accttatccc accgaaagaa
     3721 caatatgatc aacgatattc aaaaaacagc cgaaggcaaa atgcagcgtt cggtcgaagt
     3781 actgaaagaa aatctggcga aagtgcgtac cggccgcgcg cataccggcc tgctcgacca
     3841 agtggaagtc gaatactggg gcagcatggt ccccgtcagc caagttgcca acgtaacgct
     3901 tctggacgcg cgcaccatcg gcgtgaaacc gtttgagggc aatatggcgg tcaaagtcga
     3961 gaaagccatc cgcgattcaa acttggggct gaacccggca gctgtcggcg acctgatccg
     4021 cgtgccgatg cccatgctga ccgaagaacg ccgcaaagac ctgattaaag tcgtacgcgg
     4081 cgaagcggaa gaaggccgcg tctccatccg caacgtgcgc cgcgatgcca acgaccacat
     4141 caaaaaactc ctcaaagaca aagaaatttc cgaagacgag gcacgtcgcg gcgaagaagc
     4201 ggttcaaaaa ctgaccgaca aatacattac cgaagccgac aaactcctga ctgccaaaga
     4261 agaagatttg atggcaattt aacctgcacg gttcggcgtt cagacggcat ttgaatgccg
     4321 aaccgcgaaa ggcagacatg aaaagcagca cgcaggccgt tttggaacac accgccattc
     4381 ccaagcatat cgccgtgatt atggacggca acggccgttg ggcgaaaaaa cgtttcctcc
     4441 cgcgcataat gggacacaaa cgcggtttgg acgcattgga aaatatggtg aagcattgcg
     4501 ccaaactggg tgtgcaatat ctgaccgtgt ttgccttttc aaccgaaaac tggcgccgcc
     4561 ccgaagacga agtttcgttc ctgatggggc tgtttttaca ggctttgcaa aaacaggtac
     4621 gccgcctgca cgaaaacaat atgcgcctga aaatactggg cagccgcgaa cgcttcagcc
     4681 gtcaaatcct gaagggcatc gaagaggcgg aggcgttgac ggcaaacaat accggtctga
     4741 ccctgagcat tgccgccgat tacggcggca gatgggacat tttgcaggcg gcaaacaaac
     4801 tgattgccaa aggcgtatcc gagattacgg aagacacgct ggcgaaacac ttgatgctgg
     4861 gcgatgcgcc cgagccggat ttgttcatcc gcaccggcgg cgaaacgcgc atcagcaatt
     4921 tcctactctg gcaaatggct tatgccgaac tgtatttcac cgatattttg tggcccgatt
     4981 ttgacggcaa ggctttggac gatgccgtcg cttcgttcca aaaacgcgaa cggcggttcg
     5041 gacgcacctc cgagcaactg cctatcgaac agcaaaggaa ctgaatatgc tgaaacaacg
     5101 ggtaataacc gccatgtggc tgctgccgct gatgctgggc atgctgtttt acgcgccgca
     5161 atggttgtgg gctgcatttt gcggactgat tgccctgatt gccttgtggg aatatgcccg
     5221 tatgggcggt ttgtgcaaaa ttaaaaccaa ccattacctc gccgcaacct tggttttcgg
     5281 cgtggttgcc tatgcgggcg gctggatgct gcctaatttg gtttggtatg ttgttttggc
     5341 attttggctc gccgttatgc ctttatggtt gagattcaaa tggaggctca acggcggttg
     5401 gcaggtttat gccgtcggct ggcttctggt catgccgttt tggttcgcgc tcgtatccct
     5461 gcgcccgcat cccgatgatg ccctgccgct gctcgccgtg atgggtttgg tgtgggttgc
     5521 cgatatttgc gcgtatttca gcggcaaggc gttcggcaaa cacaaaatcg caccggcaat
     5581 cagccccggc aaaagctggg aaggtgcaat cggcggcgcg gtttgcgtgg ccgtgtacat
     5641 gaccgccgta cgaagtgccg gctggctggc attcgataca ggctggttcg ataccgtgtt
     5701 aatcggtttg gtgttgaccg ttgtcagcgt atgcggcgac cttttggaaa gctggctcaa
     5761 gcgcgcggca ggcatcaaag acagcagcaa cctgctgccc ggacacggcg gcgtgttcga
     5821 ccgtaccgac agcctgattg ccgttatcag cgtctatgcg gcgatgatgt cggttttaaa
     5881 ttgattctat gccgtctgaa aacgcttcag acggcatccg gtataaagtt atccccatta
     5941 tgacaccaca agtcctgacc atattaggca gtaccggcag cataggcgaa agcacgctgg
     6001 acgttgtctc ccgccacccc gaaaaattcc gcgtattcgc gctggcaggg cataagcagg
     6061 tcgagaaatt ggcggctcaa tgtcaaacgt tccgccccga atatgccgtc gttgccgatg
     6121 cggaacacgc cgcccggctt gaagccctgt tgaaacgcga cggcacggcg acgcaggttt
     6181 tacacggcgc gcaggcattg gttgacgttg cgtctgccga cgaagtcagc ggtgtcatgt
     6241 gcgccatcgt cggggcagtg gggctgcctt ccgcgctcgc agcggcgcaa aaaggcaaaa
     6301 ccatttatct ggcgaacaaa gagacgctgg tggtttccgg cgcgttgttt atggaaaccg
     6361 cccgtgcaaa cggcgcggca gtgctgcccg tcgacagcga acacaacgcc gttttccaag
     6421 ttttgccgcg cgattacgcc ggccgtctga acgaacacgg catcgcttcg attatcctga
     6481 ccgcttccgg cggcccgttt ctgaccgccg atttaaacac gttcgacagc attacgcccg
     6541 accaagcggt caaacacccc aattggcgta tgggacgcaa aatctccgtc gattccgcca
     6601 ccatgatgaa caaaggtttg gagctgattg aagcgcattg gctgttcaac tgtccgcccg
     6661 acaaactcga agtcgtcatc catccgcaat ccgtgataca cagcatggtg cgctaccgcg
     6721 acggctccgt gttggcgcaa ctgggcaatc ccgatatgcg aacgcccatc gcttattgtt
     6781 tgggtttgcc cgagcgcatc gattcgggtg tcggcgacct ggatttcgac gcattgtccg
     6841 cgctgacctt ccaaaagccc gactttgacc gcttcccctg cctgaagctc gcctatgaag
     6901 ccatgaacgc aggcggagcc gcgccctgcg tattgaacgc cgccaacgaa gccgccgtcg
     6961 ccgccttttt ggacgggcag attaagttta ccgacattgc caaaaccgtc gcccattgtc
     7021 tttcacaaga cttttcagac ggcataggcg acataggggg gctcttggcg caagatgccc
     7081 ggacacgcgc acaagcgcgg gcatttatcg gcacactgcg ctgatgccgt ctgaacaccg
     7141 ttatcaaagg aaaaccattt gcacaccctt ctagctttta tcttcgccat cctgattttg
     7201 gtcagcctgc acgagttcgg acactacatc gttgccagat tgtgcggcgt caaagtcgta
     7261 cgcttttccg tcggcttcgg caaaccgttt ttcacccgaa agcgcggcga caccgaatgg
     7321 tgcctcgccc cgattccgtt gggcggttac gtcaaaatgg tcgatacgcg cgaaggcgaa
     7381 gtatcagaag ccgatttacc ctacgctttt gacaaacaac accccgccaa gcgcatcgcc
     7441 atcgtcgccg ccggcccact gaccaacctc gcactggcgg ttttgctgta cggactgagc
     7501 ttttccttcg gcgtaaccga actgcgcccc tacgtcggca cagtcgaacc cgacaccatt
     7561 gccgcccgcg ccggcttcca aagcggcgac aaaatacaat ccgtcaacgg cacacccgtt
     7621 gcagattggg gcagcgcgca aaccgaaatc gtcctcaacc tcgaagccgg caaagtcgcc
     7681 gtcggcgttc agacggcatc aggcgcgcaa accgtccgca ccatcgatgc cgcaggcacg
     7741 ccggaagccg gtaaaatcgc aaaaaaccaa ggctacatcg gactgatgcc ctttaaaatc
     7801 acaaccgttg ccggtggcgt ggaaaaaggc agccccgccg aaaaagcagg cctgaaaccg
     7861 ggcgacaggc tgactgccgc cgacggcaaa cccatcgcct catggcaaga atgggcaaac
     7921 ctgacccgcc aaagccccgg caaaaaaatc accctgacct acgaacgcgc cggacaaacc
     7981 cataccgccg acatccgccc cgatactgtc gaacagcccg accacaccct aatcgggcgc
     8041 gtcggcctcc gtccgcagcc ggacagggcg tgggacgcgc aaatccgccg cagctaccgt
     8101 ccgtctgttg tccgcgcatt cggcatgggc tgggaaaaaa ccgtttccca ctcgtggaca
     8161 accctcaaat ttttcggcaa actaatcagc ggcaacgcct ccgtcagcca tatttccggt
     8221 ccgctgacca ttgccgatat tgccggacag tccgccgaac tcggcttgca aagttatttg
     8281 gaatttttgg cactggtcag catcagcctc ggcgtgctga acctgctgcc cgtccccgtt
     8341 ttggacggcg gccacctcgt gttttatact gccgaatgga tacgcggcaa acctttgggc
     8401 gaacgcgtcc aaaacatcgg tttgcgcttc gggctcgccc tgatgatgct gatgatggcg
     8461 gtcgccttct tcaacgacgt tacccggctg ctcggttaga ttttacgttt cggaatgccg
     8521 tctgaaaccg cattccgcac cacaaggaac tgacaatgaa actgaaacag attgcttccg
     8581 cactgatggt cttgggcata tcgcctttgg cacttgccga cttcaccatc caagacatcc
     8641 gcgtcgaagg cttgcagcgt accgagccga gcaccgtatt caactacctg cccgtcaaag
     8701 tcggcgacac ctacaacgac acacacggca gtgccatcat caaaagcctg tacgccaccg
     8761 gtttctttga cgacgtacgc gtcgagactg cggacgggca gctcctgctg accgttatcg
     8821 aacgccccac catcggctcg ctcaacatca ccggcgcaaa aatgctgcaa aacgacgcca
     8881 ttaagaaaaa cctcgaatcg ttcgggctgg cgcagtcgca atactttaat caggcgacac
     8941 tcaatcaggc agtcgccggc ctgaaagaag aatacctcgg gcgcggcaaa ctcaatatcc
     9001 aaatcacgcc caaagtaacc aaactcgccc gcaaccgcgt cgacatcgac atcacgattg
     9061 acgagggcaa atccgccaaa atcaccgaca tcgaatttga aggcaaccaa gtctattccg
     9121 accgcaaact gatgcggcag atgtcgctga ccgaaggcgg catttggaca tggctgacac
     9181 gaagcaacca attcaacgag cagaaatttg cccaagacat ggaaaaagta accgacttct
     9241 accagaacaa cggctacttc gatttccgca tcctcgatac cgacatccaa accaacgaag
     9301 acaaaaccaa gcagaccatc aaaatcaccg tccacgaagg cggacgtttc cgttggggca
     9361 aagtctccat cgaaggcgac accaacgaag tccccaaagc cgaactggaa aagctgctga
     9421 ccatgaagcc cggcaaatgg tacgaacgcc agcagatgac cgccgttttg ggtgagattc
     9481 agaaccgcat gggctcggca ggctacgcat acagcgaaat cagcgtacag ccgctgccga
     9541 acgccgaaac caaaaccgtc gatttcgtcc tgcacatcga accgggccgg aaaatctacg
     9601 tcaacgaaat ccacatcacc ggcaacaaca aaacccgcga cgaagtcgtg cgccgcgaat
     9661 tgcgccaaat ggaatccgcg ccttacgaca cttccaagct gcaacgctcc aaagagcgcg
     9721 tcgagctttt aggctacttc gacaatgtcc agtttgacgc cgtcccgctt gccggcacac
     9781 ccgacaaagt cgatttgaac atgagcctga ccgaacgttc caccggctcg ctcgacttga
     9841 gcgcgggctg ggtacaggat accggcctgg tcatgtccgc aggcgtttcc caagacaacc
     9901 tgttcggtac gggcaagtcg gccgccctgc gcgcctcacg aagcaaaacc acgctcaacg
     9961 gctcgctgtc gtttaccgac ccgtacttca cggcagacgg ggtcagccta ggctacgatg
    10021 tttacggaaa agccttcgac ccgcgcaaag catcgaccag catcaaacaa tataaaacca
    10081 ccacggcagg cgcaggcatc cgcatgagcg tgcctgttac cgaatacgac cgcgtgaatt
    10141 tcggtttggt ggcagaacac ctgaccgtca acacctacaa caaagcgccc aaacactatg
    10201 ccgactttat caagaaatac ggcaaaaccg acggcacaga cggcagcttc aaaggctggc
    10261 tgtacaaagg taccgtcggc tgggggcgca acaaaaccga cagcgcgtta tggccgacgc
    10321 gcggctacct gacgggcgtg aacgccgaaa tcgccctgcc cggcagcaaa ctgcaatact
    10381 actccgccac ccacaaccaa acctggttct tccccttaag caaaaccttc acactgatgc
    10441 tgggcggcga agtcggcatt gcgggcggct acggcaaaac caaagaaatc cccttctttg
    10501 aaaacttcta cggcggcggc ctgggttcgg tgcgcggata cgaaagcggc acactcggtc
    10561 cgaaagtgta tgacgaatac ggcgaaaaaa tcagctacgg cggcaacaaa aaagccaacg
    10621 tctccgccga gctgctcttc ccgatgcctg gcgcgaaaga cgcgcgcacc gtccgcctga
    10681 gcctgtttgc cgacgcaggc agcgtgtggg acggcaaaac ctacgacgac aacagcagtt
    10741 ccgcgaccgg cggcagggtt caaaacattt acggcgccgg caatacccat aaatccacct
    10801 ttaccaacga attgcgctat tccgccggcg gcgcggttac ctggctctcg cctttaggcc
    10861 cgatgaaatt cagctacgcc tacccgctga agaaaaaacc ggaagacgaa atccaacgct
    10921 tccaattcca actcggcacg acgttctaat cccgcaaatg ccgtctgaag cccttcagac
    10981 ggcatttcgc ggcaacattc gaaggagttt taccatgacc cgtttgaccc gcgcgtttgc
    11041 cgcggctctg atcggtttgt gctgcaccgc aggcgcgcac gccgacacct tccaaaaaat
    11101 cggctttatc aacaccgagc gcatctacct cgaatccaag caggcgcgca agattcaaaa
    11161 aacgctggac agcgaatttt ccgctcgtca ggacgaattg caaaaactgc aacgcgaagg
    11221 tctggatttg gaaaggcagc ttgccgaagg caaactcaga aacgcaaaaa aggtgcaagc
    11281 cgaagaaaaa tggcgcgggc tggtcgcagc gttccgcaaa aaacaggcgc agtttgaaga
    11341 agactacaac ctccgccgca acgaagagtt tgcctccctc cagcaaaacg ccaaccgcgt
    11401 catcgtcaaa atcgccaaac aggaaggtta cgatgtcatt ttgcagaacg tgatttacgt
    11461 caacacccaa tacgacgtta ccgacagcgt cattaaagaa atgaacgccc gctgaccctt
    11521 tcagacggca taccgaacag gaaaaccatg attccggcca cctacaccct gtcccaaatc
    11581 accgcgcggc tcggcggcga atggcgcggc gaggacattt ccgttaccgc cgtgcgcccg
    11641 ctcgcagacg cgcaggcgga acacatcagc ttccttgcca atccgaaata caaagccgaa
    11701 gtccacgaca gcagcgcggg cgcggtcatc gtttccgcca aagcggcaga cggatttgaa
    11761 gggcgcaacc tgattgtcgc cgacgacccc tatctctatt tcgccaaagt cgcccgcctg
    11821 ttttcacccg tcgtcaaagc gcacggcggc atccatccga ccgccgtcgt cgaaccgagt
    11881 gccaccgttc ccgcaagctg cgaaatcggc gcgaacgcct acatcggcgc aaacaccgtg
    11941 ctcggcgaag gctgccgcat cttggcaaac gccgtcgtcc aacacgattg caaactgggc
    12001 gacgaagtcg tcatgcaccc caacgccgtc gtttattacg gctgtacact gggcagacgc
    12061 gtcgaaatcc acagcggcgc agtcatcggc gcggacggtt tcggactcgc cttcgccggc
    12121 gattcgtggt ttaaaatccc gcaaaccggc gcggtaacgc tgggcgacga cgtggaaatc
    12181 ggctcgaaca ccaacatcga ccggggcgcg atgagcgaca ccaccgtcgg caacggcacc
    12241 aaaatcgaca accaagtcca aatcggacac aactgcaaaa tcggttcgca caccgtcatc
    12301 gccgccaaaa ccggcatctc aggcagcgta accataggca gctactgcat catcggcggc
    12361 ggtgtcggca cggtcggaca catcgaaatc gccgacaaaa ccaccatcgg cggcggcacg
    12421 tccgtcaccc acagcattac cgaaagcggc aaacacctcg ccggcatctt cccgatgtcc
    12481 acccataaag aatgggcgcg caacgccgtc catatccacc gcttaagcga aatgaacaaa
    12541 cgcctcaaaa cgctggaaca gcagctttca gacggcaaag acacgcaata aatccaaacc
    12601 gattttattc aaggaatacg acagacatgg acgtacaact ccccatcgaa gccaaagaca
    12661 tccaaaaact catcccccac cgctatccgt ttctccagct cgaccgcatc accgccttcg
    12721 agtccatgaa aaccctgacc gccatcaaaa acgtcaccat aaacgaaccc cagttccaag
    12781 gccatttccc cgacctgccc gtgatgcccg gcgtactcat catcgaagcg atggcgcagg
    12841 cgtgcggcac gttggcgatt ttgagcgagg gcgggcgcaa agaaaacgaa ttcttcttct
    12901 tcgccggcat agacgaagcc cgcttcaaac gccaagtcat ccccggcgac caactcgtct
    12961 ttgaagtcga gctgctgacc agccggcgcg gcatcggcaa attcaacgcc gttgccaaag
    13021 tagatgggca agtcgccgtc gaagccgtga ttatgtgcgc caaacgcgtg gtttgagcgt
    13081 tcggaaaaag gtcgtctgaa agtttcagac gacctaataa aaagaaaacc caccggttta
    13141 ggtttattca aacccaggga cactgtgggg attagtttta cctaactgta tcaaatatag
    13201 tttgaaacgt caatttaaaa ataaagaatc aggtgttagc gtggattata aggaatgctg
    13261 tagtattttt tctgatttca gaatggaacg gtataaaaat gccgttgggg aagacaaagc
    13321 agctgaattg tatttattga atttatcttt atcaagagaa ctttttcatg ttgtttcaat
    13381 atttgagatt gttttaagaa acaaaattga catttgcctt cagcaggcgt ttaaagacag
    13441 aaactggtta tataacagca tacagccaca gacgaatccc gcattaaaat atcaaggttg
    13501 ctttttaaga aatggcacga aagaatctgc tgaattaatc aaggttgcat tatctaaaat
    13561 acaaaataat agcgggggaa agtttgacca caaccaattg gttgcaggac taggattcgg
    13621 tttttggcgg tatctgtttg caggagggaa agatgcccaa tttgatgcta cagggaaagt
    13681 attgatgaaa gtttttccaa aaaagcccaa atctacacca agtgtgcaat ataaccaaaa
    13741 atggattttt cgagaacttt ccaatattaa caatttccgt aatcgtttgg cacatcacga
    13801 accgatttgt tttaaaggtg caataaaaga taccaactac gctagaaata ttcaccaatc
    13861 tatttttgaa ctgcttaatt acatggatgt agatactgct tcagta
//
LOCUS       DAXHKX010000032        13389 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHKX010000032 DAXHKX010000000
VERSION     DAXHKX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.032"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(110..538)
                     /locus_tag="ACFMWZ_000789"
     CDS             complement(110..538)
                     /locus_tag="ACFMWZ_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC5934781.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="ACFMWZ_000790"
     CDS             complement(603..2351)
                     /locus_tag="ACFMWZ_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC5934782.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQNY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPFGMTDADDAYTTLLGLFSHEYFHSWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     IQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTIMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="ACFMWZ_000791"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="ACFMWZ_000791"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC5934783.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="ACFMWZ_000792"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="ACFMWZ_000792"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC5934784.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="ACFMWZ_000793"
     CDS             4754..4924
                     /locus_tag="ACFMWZ_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC5934785.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..5859
                     /gene="ccoP"
                     /locus_tag="ACFMWZ_000794"
                     /pseudo
     CDS             4948..5859
                     /gene="ccoP"
                     /locus_tag="ACFMWZ_000794"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5935..6279
                     /locus_tag="ACFMWZ_000795"
                     /pseudo
     CDS             5935..6279
                     /locus_tag="ACFMWZ_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6481..7890
                     /locus_tag="ACFMWZ_000796"
     CDS             6481..7890
                     /locus_tag="ACFMWZ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC5934786.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGKARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGAVGMVPPAQKIKLPVWWAMAVPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7992..11201
                     /gene="recC"
                     /locus_tag="ACFMWZ_000797"
     CDS             7992..11201
                     /gene="recC"
                     /locus_tag="ACFMWZ_000797"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC5934787.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIVRIWRQPATVDEWVGRCRGLLETLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQSVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHAEQIAEIYIEARREGQDFSQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLSNLYQCGQVFYAY
                     GKPNAPQRVAFLLEHLIFCAVMPSETETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="ACFMWZ_000798"
     CDS             complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="ACFMWZ_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC5934788.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFELSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13173..13385
                     /locus_tag="ACFMWZ_000799"
     CDS             13173..13385
                     /locus_tag="ACFMWZ_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934789.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3359 a         3185 c         3560 g         3285 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg ctcggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gatattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattatcgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtatg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 atgccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataaggggcg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgagtgaa aatattcatg ggagaaaagt
     1561 ccgagcaatg tggtgtaggc atcgtcggca tcggtcatac cgaacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggcg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata attttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatagatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggatgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccggt cagcagaata accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacgcgat ggatgaaatc
     6901 cacagcgata tgatgctcgg ttcgacaggt gattatcttt tggaaacggc tgcatcgctg
     6961 acgattatca tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgccaaa aggcaaggcg cgttcttggt ggcggaatct gcacggcgcg
     7081 tttggaactt gggtgtcgtt gattttactg ttgttctgcc tgtcgggtat tgcttgggca
     7141 ggtatttggg gcggcaagtt cgtgcaggct tggagtcagt tcccggcagg caaatggggt
     7201 gtcgaaccga accctgtttc agtcgtgccg acccatggcg aggtattgaa cgacggcaag
     7261 gttaaggaag tgccgtggat tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc cgacgagccg atgacattgg aaaccgtcga ccgttttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaagactc tatgagttac gatatgatta gtccgtttgc cgaccgcacg
     7501 gtgcatatcg accagtacag cggcaagatt cttgccgaca tccgttttga tgattacaac
     7561 ccgttcggca aatttatggc ggcgagcatt gcgctgcata tggggacttt gggctggtgg
     7621 agcgtgttgg cgaacgtcgt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgtccggc gcggtgggca tggttccgcc ggcgcaaaaa
     7741 atcaagctgc ccgtctggtg ggcaatggcg gtgccgctgc tgctgattgc attgcttttc
     7801 ccgaccgcgc tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga cccgatgccg tctgaagcgt tcagacggca
     7921 ttttgtttga aggcgggcgg gggaaaggct atataatccc gaatacttga ccacagcttc
     7981 tgtttgaaat catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcactgt
     8041 ttgcccgcat tcaaaaagtc aaaccgctga aatcggtttt acagcccgaa cagattattg
     8101 tgcagagtca ggggatgcgc cgctacctca atacctgcct cgcccgcgat ttgggcgtgg
     8161 cggcgaattt gtcgttcagc ctgcccgccg gtctgacgtg gaagctgatg aaaaaactga
     8221 ttcccggtat tccggaactc agcccgtttg cgcctgaagt gatgcgctgg cggctgttgg
     8281 atttgttccg cagcgaggca tttcggaata cggcagaatt tgaagatgtg aggaatgtgc
     8341 tgcaagacta tctaggcagc ggcgaatcgg cagattatca gcttgcggga cagcttgcgg
     8401 acatattcga ccaatacctt gtctatcgtc cgcagtggat agacgcgtgg cagcagggca
     8461 ggatactcag tttgggcgac gatgaaatct ggcagtccaa actgtggcgt tatcttgatg
     8521 acggcaggca gagcgcgccg caccgtgttg cgttgtggga aaagctgttg gaatctttga
     8581 gcagtgataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcgccgatgt
     8641 atttgcagct tttgcacaag ctgtccgaac attgcgatgt gttcgtgttt gcactcaatc
     8701 cgagtgggat gtattggggc aacgtcatcg aagcggcgca aatcctcaaa ggcggcggcg
     8761 atcccgattt aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcgcg
     8821 actttttcga ctttttgaac gaaatggaaa tagaaggaga aacgccggta tttgaagaag
     8881 gcgggcgcga tacgcttttg cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt
     8941 ctgaaaatgt gggaagcgtc ggtacggatg acggttcgat acgtatcgtc tccgcgcaca
     9001 gccctttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaacatc
     9061 ccgattggca gccgcatgat atcgccgtat taaccccgaa tatcgaatcc tatacgcctt
     9121 ttatcgaggc cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgttt
     9181 cggacgtgaa aataagccgc cgacaaccgc tgtttcatgc tttgtcatgc ctgttcgact
     9241 tgttggaaag ccgatttgaa gtcgataaag tgcttgtgct tttagaaacc gcacccgtgt
     9301 tgcgccgttt cggactgact gaggacgatt tgccgctttt gcacgacatg gttgccgatt
     9361 tgaacgtccg ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgtttacct
     9421 ggaagcaggc ggtagaacgc atgatattgg gctggatgct gcccgaaggc ggcaatccga
     9481 tgtggcagga tgtcagcgcg tggtatgccg acgtgaatca aaccgccatg ttcggacgtt
     9541 ttgccgcctt cctcgaaacc ctgtcggata ttgtacggat atggcggcaa cctgcgacgg
     9601 ttgatgaatg ggtaggacgt tgccgaggtt tgcttgaaac attgttccaa gccgaagccg
     9661 atgaccaaaa ggcagtccaa aacctcgaaa acgaatgggt caaatggcag gcggaaacag
     9721 aattggcgca attttccgga cagttgccgc aacaaaccgt catccgccat atccgacgtt
     9781 tcctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg
     9841 tgccgatgcg gagcctgccg ttcaaagtca tctgcctgtt gggtttgaac gacggagatt
     9901 ttccccgcaa taccaaagcc gccgtattcg acctgattgc caaacatccc gccaaaggcg
     9961 accgagcccg tcgcgatgac gaccgctacc tgttccttga agctctcatc agcgcgcgtg
    10021 aaatcctgta tttgtcctac atcgggcgcg atatccgcaa agacgaagag cttgccccgt
    10081 cttcgctgtt gggcgaactc atcgacacag ttgccgccat gaccggcaca aacagccgcc
    10141 aacttgcaca aaattggata gaacagcatc cgctgcaagc cttctcgcgc cgatatttcc
    10201 aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc gccgcgctcg
    10261 gacaaacgcc cgaaccgcca caaccctttt tcgaccaatc cgtagaaaac gccgaacccg
    10321 ttgccgaaat cggacaggac gaatttatcc gcttctggcg caaccccgtc aaagtatggc
    10381 ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac
    10441 ccttcgaacc gcaacatgcc gagcaaatcg ccgaaatcta tatcgaggcg cgccgcgaag
    10501 gacaagattt ttctcaaacc gccgcccgca tcggggcgga aagcctgctg ccttccggag
    10561 aattgggcag actttggcag caggacttcc aaaccgctgc caaacaaatt gacacggcgg
    10621 ttttaaacag tcccaaactt cctccgctgc cctacgccat accgtcggac gggcaaatcc
    10681 tgaaaggcag tttgagcaat ttataccaat gcggacaagt gttttacgcc tatggcaaac
    10741 ccaatgcgcc gcaacgtgtg gcttttttgt tggaacacct gatattttgc gccgttatgc
    10801 cgtctgaaac cgaaacgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg
    10861 cggaaatcgc gcaagacagg gcattgcagc tattgtcgga atggatggcg tttttcaata
    10921 tcgggcaaaa ccgcccgctg ccgttttttg ccaagacctc gcttgccgct gccgaagcgt
    10981 ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc tattacggca
    11041 acaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa
    11101 gccaaaaccc actcgaacaa cccctgtttg aaaacctcgc ccgcctgctt gccgacacgc
    11161 ttgccgcagc ggaaaaaagg gaaggggccg gagcagcctg acgggatgga aaggcaaatg
    11221 ccgtctgaaa acagcaaaaa ggttttcaga cggcattttg tttatcgtgt ttaaggtgcg
    11281 ggatagaatg tgggcgcgcc gggaccgacg ggcaggccca aaacaaatac ccaaatgcag
    11341 aataaggcaa tccacgcaat caagaagaaa gcggaatacg gcaacatcat agaaatcagc
    11401 gtacccacgc ccgcatcttt tttgtatttg atcaccgtcg ccataatcag cccgaaataa
    11461 ctcatcatcg gcgtaataat attggtaacg gaatcaccga tgcggtaagc ggcttgaatg
    11521 acttcgggcg cgtagccggc caacatcagc atagggacga aaatcggcgc agttaccgcc
    11581 cattgcgcgg aggcggagcc tatcatcaga ttgataaaag cacaaattaa aataaaaccg
    11641 ataaacaaca cgctgccgcc caagccgact tcttttaaga acgtcgcccc tttaacggca
    11701 atatattgcc caatattcgt ccaattaaaa aatgcgacaa actgtgcggc aaaaaagatg
    11761 atgaccaaat aaagccccag agtactcatc gattcggcca tcgcattaac gacttcctgt
    11821 tcgccgcgca aacttcgggt tacccggcca taaacaatgc ccggcagtgc aaacaacaag
    11881 aaaataaaaa caacaattga ttttaaaaac ggcgaaccgg aaaccaatcc tgtttcagga
    11941 tgacgcaaaa taccgtcggc agggacgatg ctccaagcca atagggcgga taaggcaaca
    12001 aacaccacgc cagcccaaat taatccttta tattccaaag gcgtgatttc attggaatgt
    12061 cgaatgtctt tttcttcttg tgacaaatct gattgataag ggcccaattg cggttcgacg
    12121 attttttcag taacaaaata accaatcaaa gcaatcacaa acgtactggc taccataaaa
    12181 aaccagttgg cttcagggcc tacgacgtag tcgggatgga tgatttgcgc cgcctgttgg
    12241 gtgatgcctg ccaagagcgg atcgattgtg cctaagaaca gattggccga ataaccgccc
    12301 gaaacgccgg cgaaagccgc agccagaccg gcaagcggat ggcggccgag ggaatgaaag
    12361 atgatggcgg acaaagggat taggacgaca tagcccaatt cagaagcggt attagataaa
    12421 atccctgtaa aaacaaccat aaaagtagtg agtttgcgtg gagattttgt gagcaataag
    12481 cgcattaatg cggaaatcaa gcccgatttt tccgcaatcc ccacgcccaa taaagaaacc
    12541 aacaccgttc ccaacggcgc gaaaccggtg aaatttttaa cggtatgcgt caggattttg
    12601 atcaaaccgt cagcatcgag caggctgaca acgtgaatca aaccgtcatc ggcacgtcct
    12661 ttcgcaccaa cagggcgcgg atcggggacg gatagttcga aatacgcacc ggcggcagag
    12721 gcaatcagca ataacacaat gaaaataata aaaagcgtaa ccgggtgcgg caacatattg
    12781 cccagccatt cgactgtgcg taaaaatcgt ccgtcccgtt gcgtatcggt ttgactcatc
    12841 tgcttctcct taaaaaaaat atattgagtg cattaaattt ttgaatgtaa catgaagtgt
    12901 tttgtaagca aactaaaata atcgtatatg tatataaccg taatagatta aaacagaaaa
    12961 tgcccacatc gttattctca taaaggcggt aatccgaaaa cttgacgtta aagccttatc
    13021 aggaatgact ggaactcaaa aaaacggatt cccgcctgcg cgggaatgac gggattttcg
    13081 gtttttgttt gcaagaatgc cggagaagaa tgcagtttac attttgaatg gtattgttat
    13141 taattgtaaa taaatattgg aaggtaaatg atatgaaaaa tgaaattcaa aaaatcatgg
    13201 acaaatataa tccatggcat gaagatgatt ttgagtcgta tgaagacatt gccagagatg
    13261 tatcgctgac gacagataaa acgttcattg aacattattt gttagaagtt tattcagaag
    13321 aaaacggaca ttttgaccaa gagaatgtcc atgcaatgat agaagaaatt aaaaatgcaa
    13381 tttagtgaa
//
LOCUS       DAXHKX010000033        13020 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHKX010000033 DAXHKX010000000
VERSION     DAXHKX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.033"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(630..809)
                     /locus_tag="ACFMWZ_000800"
     CDS             complement(630..809)
                     /locus_tag="ACFMWZ_000800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934790.1"
                     /translation="MRHFFIRQHRRRIIKRRAHPPGVRQLHRIAHCLRRPVLCGKPFY
                     GFLFESNPLRLIEAV"
     gene            complement(770..1294)
                     /locus_tag="ACFMWZ_000801"
     CDS             complement(770..1294)
                     /locus_tag="ACFMWZ_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFC5934791.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSATSA
                     ALCGTSSSVSTAAA"
     gene            1605..2357
                     /locus_tag="ACFMWZ_000802"
     CDS             1605..2357
                     /locus_tag="ACFMWZ_000802"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC5934792.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQHLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            2548..2623
                     /locus_tag="ACFMWZ_000803"
     tRNA            2548..2623
                     /locus_tag="ACFMWZ_000803"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2581..2583,aa:Asn,seq:gtt)
     gene            2754..3764
                     /gene="waaF"
                     /locus_tag="ACFMWZ_000804"
     CDS             2754..3764
                     /gene="waaF"
                     /locus_tag="ACFMWZ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC5934793.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            3819..4265
                     /gene="smpB"
                     /locus_tag="ACFMWZ_000805"
     CDS             3819..4265
                     /gene="smpB"
                     /locus_tag="ACFMWZ_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC5934794.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            4631..6673
                     /locus_tag="ACFMWZ_000806"
     CDS             4631..6673
                     /locus_tag="ACFMWZ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC5934795.1"
                     /translation="MYYETRSKQASKQKFKKSFIISLFFSILYTSPLLAVDYVYDNSK
                     LTNEQIDRLKKLRDKSSEYWKQETYLLKPTNGSSQNSLKVPALYSKTHQFENINNSKK
                     ISFYDKEYTEDYLVGFARGFGVEKRNGEEEKTLQQYFKDCVNTGNSNNDQCEQPSFGN
                     SGPRLIKSDIFALAPQIKNSHINSEILSVGNYIEWLRPTLNKLPSWQEHLYSGLDPFH
                     YIEVTDNSHVIGQTISLDEFRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKE
                     DYAGGARFRFAYGLKDKVPETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNG
                     TADKHAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFALRLN
                     DKNSDIFDRTLPRKGLWLRVIDGHSKQWVQGKTTPVEGNRKGVQLGGEVFTWQNESNQ
                     LSVGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQY
                     QRFRHRINTEDATERFTSKGITASIEAGYNALLAEHFTEKGNRVRFYLQPQAQLTYLG
                     VNGKFSDSENAQVNLLGFRQLQSRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGV
                     EMDGERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(6854..8221)
                     /locus_tag="ACFMWZ_000807"
     CDS             complement(6854..8221)
                     /locus_tag="ACFMWZ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC5934796.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYKHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(8331..8612)
                     /locus_tag="ACFMWZ_000808"
     CDS             complement(8331..8612)
                     /locus_tag="ACFMWZ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934797.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPATEAPAAEAPAAEAPAAEAPATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            9086..9568
                     /locus_tag="ACFMWZ_000809"
     CDS             9086..9568
                     /locus_tag="ACFMWZ_000809"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC5934798.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            9655..10467
                     /gene="hpnC"
                     /locus_tag="ACFMWZ_000810"
     CDS             9655..10467
                     /gene="hpnC"
                     /locus_tag="ACFMWZ_000810"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC5934799.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(10538..11008)
                     /locus_tag="ACFMWZ_000811"
     CDS             complement(10538..11008)
                     /locus_tag="ACFMWZ_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC5934800.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            11099..12940
                     /locus_tag="ACFMWZ_000812"
     CDS             11099..12940
                     /locus_tag="ACFMWZ_000812"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC5934801.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         3134 a         2911 c         3640 g         3335 t
ORIGIN      
        1 cattgatttt tatgtagatt tttttattta aacggcaatc cattccctct ttcgtgggag
       61 agggctaggg agagggcatc ggggcggtaa cggaaatatt gtctgtatct gtcccgtttt
      121 accctccctc cgaccctccc ccacaaggga gggagccaag ttgcaggcac gccgacggaa
      181 tttggcttcg cattaactgc cttattttga acgcacgtca aacttaaatt tgcaatgccg
      241 tctgaaaaga agatgggctg tcggataagg atgtccgacc atttgcttag acctaacgtg
      301 ggtttgagat atagtggatt aaatttaaac cagtacggcg ttgccttgcc ttgccgtacg
      361 atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatactttc
      421 atacgcgcga acgattgggt gctttgggcg gcatcgtgca acatcatacc gccttgtacc
      481 gcagcgtggc ggtatatgaa ccagctgtgc ttatcgtgcg gcgcggaagc aagaagctgc
      541 ggtgggcgag gcgcgaattg aggattgcgg cgggtgaggc ggtcgccttg gcaggcgggc
      601 agacgtttga cgttattaat attcccgatt cagacggcct ctatcaggcg caatggattg
      661 ctttcgaaca ggaagccata gaaaggtttg ccgcacaata cgggacggcg caggcagtgt
      721 gcgatgcggt gaagctgccg caccccgggc ggatgggcgc ggcgtttgat tatgcggcgg
      781 cggtgctgac ggatgaagaa gtgccgcaca atgcggcgga ggtcgcgctt tgcggcgtgt
      841 tggcgtggtt gcagcatgac ggcatcggtt ttgccgtgta cgaaggcgtc aacctgatgc
      901 ggcaaatccg caaactgatt accgccgata tggctgcgga ctggtcgtcg gcgatgttgg
      961 cgcagcggct taattgcagc gaggtcgcgt tgcggcggtg gacatcggcg ggaatgtcgg
     1021 cgaccacccg gtgcatccag cccagtccgg tcggcgcgtc tttatcgtaa acggtcagga
     1081 cgaaactttt tgtcccggcg ggcggatttt tccacgacag cgcgggcgaa gcattgccgc
     1141 ccgaacagcc aaatccgtaa ggcgcgctca aaagctggtt ttgcgtgaag ccgccgtctt
     1201 cggacgggtt gtcgaattgc agcgtgaatg cgcccccggc aaaggcagcg gttgaaaaag
     1261 ccagcagtac ggcggagaga atgcgggttt gcatagatga ttcctttgct tgatgaataa
     1321 gtggattcat tataggctgt caacggcggc aggaatgtgc cgaaacggtt ggatgatgtg
     1381 ccagaatgcg caaatgcaaa aatcgtggtt tcagacgagc ctttgcaggt cgtctgaagt
     1441 ctttaattcc tttccctgag gctttggaaa tatcgcatat ttaggcattg ccgcaaccca
     1501 accctcaaga tttacgtcat agcccgttcc tgcatcggcg tgcgggaatg gcaaaaattg
     1561 gggattttgc cgccaaatcc atgatagaga tacggagcaa acgaatgatt accctgcctt
     1621 ccttaaacaa tctgccgtca aaatgggatg aaatccgcta ttggctcgaa acccgggttt
     1681 ttgagtgcgg cgttatgccg catcccaatc tgacggagtg cgaagccggg cagttcgatc
     1741 gggatttttg ggacaatatc ggctgcgcgc ctgaagaata tgtccgtatc cgccgagcca
     1801 tccgtctgtt ggaagcgcgt tatcccgaca gtttgaatga gctggtgtgt gccgccattg
     1861 ccacgccttt gggcgagatg ctggcggtgt ttggaaataa gggattgtgt ctgctggagt
     1921 ttgtcggaca gaagcatttg gaacaggaaa ttgccgccgt ccaaaaagcc ttgcgcggac
     1981 ggtttgtgtt tcgggaggat gagcggacgc aacttttgcg gcaggaattg gacttatact
     2041 tcaagggtcg tctgaaaacc tttgccacgc ctttggagca gattggcacc gagtttcaaa
     2101 agcaggcatg ggacgcgctc ttggcgattc cttacggtga aacgcgcagc tacaaggaac
     2161 aggcgcagca tttgggcaac cccaaagccg tccgcgccgt tgccgccgcc aacgggcaga
     2221 acaaagtatc tatcctgatt ccctgccacc gcgtcatcgg cagcgacggc aaactgaccg
     2281 gctacgcggg cgggttgaac cgcaaacagt ttttactggc tttggaacgc ggcgaagttc
     2341 agacggcatt gttttaagcg ggggagggca gaacggctca tcgggcttaa atatggtgtg
     2401 atgacggctg tggaaattca tacaacggca atccttttaa ggcatcggtc ttaatgtctt
     2461 tctgattcat atgcttgtcg ttttgtcgcc catttttcca aaattaagga ttgacacatt
     2521 ttttccggac gctataatac gccccattcc ccgatagctc agtcggtaga gcgacggact
     2581 gttaatccgc aggtccctgg ttcgagccca ggtcggggag ccagaatttt gaagcccgaa
     2641 cttgaatgtt cgggcttttt catatttgca cgtccggcgt tcggaatgcg gcgagatagg
     2701 ttttccaacg gtttcgcggt aaaattccgc ctgtctgatt tcggtttctt gttatgtcca
     2761 tcaaaatcct gattatttct cccagttgga taggcgactg cgtgatgacc cagcccttgt
     2821 tccgccgttt gaaggaactt caccccggtt gcacgattga tgtgttcgcg ccgaagtggt
     2881 cgatggcggt gttcgagcgt atgccggaag tgaatgaaat tcttgagaat ccgttcggac
     2941 acggtgcgtt ggagttgaaa cgccgctggc gggtcggcag ggatttgggg cggcgcggat
     3001 acgatcaggt tatcgtgttg cccggttctt tgaaatcggc agtcatcgcg ctagcaacgg
     3061 gtattgccaa aaggacgggt tatgtcggtg aaagccgtta ttttctgttg aacgatatac
     3121 gcaggctgga taaggaacgt ctgcctttga tggtggacag atatacggct ctcgcgcatc
     3181 cgagtcagga agattttgac gggcattcgg gattccccga gttttccatt gatgaacggc
     3241 ggcgggaaat ttctgtcgaa actttcggtt tgaatcttgg aaagcctgtt ttggcatttt
     3301 gcccgggcgc ggaattcggg ccggcaaagc gttggccgac aaggcatttt gccgagttgg
     3361 gcaaacatta tctggcggcg ggttggcagg tttggctgtt cggttcgcaa aaagatgatg
     3421 aaattgccga ggaaatcaac tgcctttcag acggtatgtg tgtcaatttg tgcggcaaaa
     3481 ccgatttgtc gcaggcaatg gatttgctgt cgttggcgga cacggttgtg tgcaacgaca
     3541 gcgggctgat gcacttggcg gcggcattgg gcaggaaggt ggtggcggtt tacggttctt
     3601 ccagcccgac gcatacgccg cctttgagcg acagggcgaa aatcgtcagc ctgcacttgg
     3661 aatgttcgcc ctgttttaag cgggaatgtc cgctggggca taccgactgc ctcaacaggc
     3721 tgtatcccga gaagattgtg caggcggttg aagaggcggt atgatgggtt ctgttttcgg
     3781 gtttatgccg tctgacggcg gatattgata ggatgtttat ggctattgcg aacaataaaa
     3841 aagcatttca cgattttttc attgaagacc ggattgaagc cggtttggtc ttggaaggct
     3901 gggaagtcaa agcaatccgc gccgcgcgcg tacagcttaa agagagttat atctattgga
     3961 aaaaagacgc gttttatctg gtcggctgcc atattacggc tttgcccaca gcctcgacac
     4021 acattaaacc agatgccgta cgtccgcgca agctcttgtt gaaccagtcg gaaatcaaca
     4081 aattaatcgg caaaaccgaa cgtgccggct acaccatcgt gccgctggat ttgcattttt
     4141 cgcgcggaaa aatcaagatg gaaatcggtt tggcaaaagg taagaaacag cacgacaaac
     4201 gtcagagtat gaaagaggcg gattggaaac gcgagaaaca gcggttgatt aagcatacgc
     4261 gctgattgtg gaaacagtgc ttcagacggt atgggcaagg ctgccgcctg atgtgtaaac
     4321 gttctacacc taaaccagca tccgctttaa tcagtacggc atgatgcccc aaaccttcgt
     4381 caaaggtttg agcaatttga tgataaattg gcattagcca agggtaaagt gcggtcattt
     4441 ttcctgaaat ttttgtaaat aacgagggca attatacaca ttttcaaact ggcttgacaa
     4501 aatagcaaaa agcaaacgtt tacttttaat aaataagaaa taaatagaga aaaatgtgag
     4561 ctaaatgaca ttttttgttc aattctatgc taaaatccac gccccattta tttttacttg
     4621 acaaaggaaa atttattatg aaactcgaag caagcaagca agcaagcaga agtttaaaaa
     4681 atcatttatt ataagtctat ttttttctat tctttatacc tctccgcttt tggctgttga
     4741 ttacgtttac gacaattcta aattaacaaa tgagcaaatt gatcgcttaa aaaaacttag
     4801 agataaaagc agcgaatact ggaaacaaga aacttatctt ctaaaaccga cgaatggtag
     4861 ctctcagaac tctctcaaag ttcccgcact atactctaaa acacaccaat tcgaaaacat
     4921 caacaactca aaaaagattt ctttttatga caaggaatac actgaagatt accttgttgg
     4981 cttcgcccga ggtttcgggg ttgaaaaaag aaatggggaa gaagaaaaaa cactccagca
     5041 atattttaag gactgtgtaa atactgggaa ttccaataat gatcagtgtg aacaaccatc
     5101 cttcggaaac tctggcccaa gattaataaa gagcgatatt ttcgccttag ctccacaaat
     5161 aaaaaatagc catatcaaca gcgaaatttt atctgtaggt aattatatcg aatggcttcg
     5221 cccaacactt aataagcttc ctagttggca agagcatcta tattctggtt tagatccatt
     5281 ccactatatt gaggttacag acaactcgca cgttatcggg caaacgatca gtttggatga
     5341 attccgctta gaaaattctc tctgggaacc ccgttgggat tctgatgttg gggaattaaa
     5401 aacaacgaat gcagacatcc gttttaatac taaaagtgaa agtttactcg taaaagaaga
     5461 ttatgctggc ggagctcgtt ttcgttttgc ttacggtcta aaagataaag tccctgaaac
     5521 accagtttta acttttgaaa aaaatataac tggcacttcc gacatcatct tcgaaaatcc
     5581 aatagacgat ttaaaaagtc tcgacgggca tcaaattatc aaagtaaatg gcacagcaga
     5641 taaacacgca ttccgtcttt ctggcaaaca ccaaaaggga atttatacgc tttctttaca
     5701 acaacgccca gaaggctttt ttaccaaagt gcaagaacgc gatgatattt cgatttatgc
     5761 acaacaagcc caagccgcca ataccttatt cgccttgcgt ttaaacgaca aaaacagcga
     5821 tatttttgac cgcactttac cgcgcaaagg cttgtggtta cgtgtgattg acggacattc
     5881 taagcaatgg gtgcaaggta aaaccacgcc agtagaggga aatcgaaaag gtgtgcaact
     5941 cggtggcgag gtgttcacat ggcaaaacga aagtaatcaa ctttccgttg gcttaatggg
     6001 cggacaagca gaacaacgta gtactttccg caacccagat acagacaatc ttacaacggg
     6061 aaatgtgaaa ggctttggtg caggcgttta cgccacttgg caccaacttc aggacaaaca
     6121 gacaggtgcg tatgcggata gctgggtaca atatcaacgt ttccgccacc gtattaacac
     6181 tgaagatgct acagaacgtt ttacttcaaa aggtattact gcctcaattg aagcaggtta
     6241 caatgcttta ttggcggaac acttcactga aaaaggcaac cgtgttcgtt tttacctgca
     6301 accacaggca caattgactt atttgggggt aaacggaaaa ttcagcgata gcgaaaatgc
     6361 ccaagtgaat ttgcttggct tccgccaatt acaaagccga gtgggcgttc aagctaaagc
     6421 gcaattttcg ctctataaaa atatcgctat tgaacctttc gccgctgtga acgcgcttta
     6481 ccacaacaaa cctttcggtg tggaaatgga cggagagcgt cgagtgataa acaataagac
     6541 ggcaattgag agccaattag gtgtagcagt gaaaatcaaa tctcacttaa ccttacaagc
     6601 cacattcaac cgccaaacag gcaaacatca tcaagctaaa caaggtgcgt tgaatttgca
     6661 gtggacgttt taagttaagt tgtaacaaaa taaaatcggc agggaaattc actgccgatt
     6721 tttttatttt aaaaatgcgg tcatttttcg tgtcatttcc accaattttt taccgcactc
     6781 acgagctcgc gctaaaccga caatcggatc tattgtccct ttctttgttg tgtatgcaaa
     6841 tcagaaatgc cggttacgga agcagtttgc ccggattcat aatgttatat ggatcaaggt
     6901 gttgtttgat gcttttcatc agggcgattt cggcaggcgt gcgtactttg tccagccact
     6961 gttttttgat gatacctatg ccgtgttcgg cggcaatcgt gccgttgcag gcaagaacgt
     7021 tgcgataaac tgcgtcgttg atgtcgtttt cgtaacgata gacttcattg ctgaggattt
     7081 cgggcaggaa agtattgtag tgcaggctgc cgtcgccaag atgtccgaag cagacgattt
     7141 gtatgccttt aaaattctgt tccaaatctt tggcgcaccg gcggacaaag tcggcaacgc
     7201 gtccgatagg aatggcaata tcgtgtttga tgctggtgcc cagtttgcgt tgcgatgcgg
     7261 agatgttttc gcgcaatgcc cacatatgga tacgttcttg ttcgctttgt gccaatacgc
     7321 tgtcggtaaa tcctttttta taaagaaatt cgacaagccg atcatcaaga ttgctgtcgg
     7381 gtaatgagtc ggtcaactca agtaaaatat gccattctga atgtgtcggc agggggaatt
     7441 tgctgaattc ggaagacaac tcggcggcaa aacggccgat cagctcaaaa ctgcataggc
     7501 gttcggcaaa gtgagcttgg gtttcggtca gcaggcggac ggcggattcg atgtcgggaa
     7561 tgccgaccca tgccgttgcc ttgtccgagg gacgggcaaa aagcttgagc gtggcggcag
     7621 tgatgatgcc cagtgtgcct tcgctgccga taaacagatg gcgcaggtcg tagccggtgg
     7681 tgtttttatg caggggatgg agatgggaaa ccagctcgcc gttggggagg acgacttcca
     7741 aaccgataac caggtcgcgc atcgtgccgt aacgcaatac gttcaaacct ccggcattgc
     7801 aggcgatgtt gccgccgatt tgacaggagc cttcgctggc gagactgagt gggaacagcc
     7861 tgtttgaggc ttcggctgcc tgttggacgg tttggagtac ggaacctgct tcgacggtta
     7921 tgcagttgtc tgacaaattg atgctgcgga tgcggttgag tttggaaagg ttcagcaata
     7981 cgccgttttc cgatactgcc gcgccgcaca aaccggtatt gccgccttgc ggcgtaaccg
     8041 gaatacggtg tttgtaacaa aaacgcataa tggtttgcac gctttcaacg ctgcgcggct
     8101 gcaaaatgat gtctggtgca gacgtaaagc ggttgcgctg gtcttttaaa agcgtcggag
     8161 aaagttcggc aatttcatct gccggcagga gtcgggaaaa ttcggtatga aggtcaggca
     8221 tgtttgtcgg aagggtaggg agaagattga ggaagggaat ctgatgccgt ctgaaaaatc
     8281 aaaaaagcag gatactgaac gtatcctgct tttttgcaag cggaaaatgc ttattttgca
     8341 gcttcggcag caggagcttc agcggcaggt gcttcggtag cggcagcttc agcagcaggt
     8401 gcttcagtag caggagcttc ggcggcaggt gcttcagcag cgggagcttc ggcggcaggt
     8461 gcttcagtag cgggagcttc ggcggcaggt gcttcagtag cgggagcttc agcggcaggt
     8521 gcttcggtag cgggagcttc agcggctttt tcaccgccgc aggctgccag gaccaaagac
     8581 aacaaagcag cggcaaacag ggattttttc attttagtaa cctttagaac caaatttaaa
     8641 ttattgaaac aaataggtct ttatcgacca tactacaaac cgggttggaa attcatcgct
     8701 gaatttgcgt aattatgcac tatggaataa gggattgcaa tgtcgtacag atgggaaaaa
     8761 ggtttgaatg aaaagttttc tggcgggcgg attgataaat cctgtaaaac ggttttgctg
     8821 ttaaaaattc gatagggata aatattatag agattaatat aatttcagta tcttgtatga
     8881 tttattaagg cggattggga aagtcggcag acaggtcgga atggttttaa gaatgttgta
     8941 aaaaagttac accgcaataa aataacaaaa atttacattg aaatatgtgc tgtacagttt
     9001 atgaacaggt taacggtaat cggccggcgc ggcggttgtg ttagaatgcg cctgtaatca
     9061 atctgctgtt cctgaaagga caaatatggc tatcgtgaaa aattccgtgg tttcgctgca
     9121 ttatgagatg tatgatgcca acaatcagct tttggacaaa accgaagaac cgattgcgta
     9181 tctgcacggc ggttacgacg gcattttccc tttggtggaa gaggcgttgc acggtaagga
     9241 tgccggcgat acggtcgatg tggcgctgtc gcccgacgat gcgttcggcg agcaggatcc
     9301 ggagttggtc cgtattgaag atgcgggcgt gttccctgtt gaagtcgaag tcggcatgat
     9361 gtttgaagcc gacgatcctg aaaccggcga tgttgtcgtc tatcgtgtaa ccgatgttgc
     9421 cgacggtaag gcggtggtgg acggcaacca tcctttggca ggcatgaaaa tccgctttaa
     9481 ggctacggtt gaaagtgtgc gcgatgcatc cgatgaggaa atcgcacacg gtcatgtcca
     9541 cggcccgcac ggtcatcacc accactgatt gccggcagct tggaggtatc tgatgccgtc
     9601 tgaaaagggt ttgttccatt tcagacggca ttttgtttga tggatggaga aggtatgtcg
     9661 gttgggcatt atgaaaattt tcccgtcggt tcgctgattt tgccgcgcag gttgaggaag
     9721 ccggttcacg cggtgtacgc gtttgcacgg acggcggacg atatggcgga cgagggcagt
     9781 atgccgtctg aagccaggtt ggcggggttg gagggtttgc ggcgcgagtt ggacgtgttg
     9841 gcatcgggcg gccggtcggc gcatcctttg attgcacgat tggatgccga ggcggttgtg
     9901 ccgttcggtt tggatttgca gccgttttat gatttgcttt ctgcattttc tcaggatgtg
     9961 gttaaaacaa ggtatggaaa tttcggcgag ctggccgatt attgccgacg ttctgccaat
    10021 cctgtcggac gcattatgct ggctttgtac gggaaaacgg atgcggtgtg cgtggcgcaa
    10081 agcgacggca tctgtacggc tttgcaactg gtgaattttt ggcaggatgt ggctgtggat
    10141 tggcaaaagg gcagggttta tattccgcag gacgatttgt tgaaattcgg tgtttccgaa
    10201 ggacagattg cggagggtag ggcggatgcg gcgtttcagc ggctgatggc gtatgagtgc
    10261 cggcgcgcat ttcgtatgct gaaggcgggt tcgcctttag cgcgcgaatt gaaagggcgt
    10321 atcggtttgg aactccgtat gattgtgtta ggggcgcagt tgattttgca gaaactggac
    10381 gcgtgccgat acgatgtgtt tgcacaacgc cccgttttgg ataagaagga ttggttgatt
    10441 atgctgaaac gcgcgttgtg gaaatgatgg gggaaatgca tgccgtctga aaggggttgt
    10501 tccgcttcag acggcatttt gcaggcggtt ggtatggtca ggcgatttca tgtgggttca
    10561 acctggcttg atcttctgca cccaagccga tgagtttgcg cagtcgggtc atgtagaaat
    10621 tgacatccaa accggtgccg tagcgttgcg ccgtccacag tgtttccgcc agtgcctcca
    10681 tcatttcgtg ttcggcttcc agccagccgc gtttggcgca cagggtgtcg tggattgcgc
    10741 gtatgccgtg cggctggtct atgcccgcct gttcttggac ggacagatgc agcgacatat
    10801 gcaggaaggg gttgctttcg ccgttttcgg gcagccagtc ggtgtccaga tggtcttcga
    10861 tgcgttcgag atagcggtgg tattcgggat gggcttcgac aatgcggagg gctttctgtt
    10921 ccagtgcgct cagttgcagc ggattgagcc gctgctgcca cacgcgggcg aaaaagcggc
    10981 ggacatcgtg ggtattgaca tcgtacatgg cgggttcgat aagatggtta tggaaatagg
    11041 ttcggataat ggcgggtttt acataaatta aatcccaccg tcaaccttac catttataat
    11101 gcagacaaat tatttgtctg aaacggattc gattatgaaa aaactgattt gcctgttcgc
    11161 cgtatttttg atgttgtgcg gacgagcttt cgcgctggat gcgaacgatc tgctgccgcc
    11221 ggaaaaggca ttcgtgccgg agcttgccgt tgccgacgac ggtgtgaacg tccgtttcaa
    11281 gattgccgac ggatactata tgtatcaggc gaaaatcgtc ggcaagaccg atccggcgga
    11341 tttgttggga cagccttctt tcagcaaggg cgaagagaag gaagacgagt ttttcggcag
    11401 gcagacggtt taccatcacg aagcgcaggt tgcctttcct tatgcaaagg ctgtcggcga
    11461 accgtataaa ttggttttga cctatcaggg ctgtgccgaa gccggcgtgt gctatccgcc
    11521 cgtggatacc gagtttgata tttccggcaa cggcacttac catccgcaaa ccgacgaacc
    11581 ggcatccgcc aaagaccgct ttttgcagcc ttcctctcaa aacggcagcg gggcgctgcc
    11641 gcccccgaag ggggatgagg gcggcgacag ccgtttcaag ctgtcttggg atacgctcaa
    11701 cgccaatctt ttggcgtttt ttctcgccgg tttgggtctg agttttaccg cctgtatgta
    11761 tcccctgttg ccgattgttt ccagtattgt ggtcggcgac aaaaaggcgg gcaaggcgcg
    11821 ggcgtttgtg ctgtccgtcg tttatgttca gggtttggct ctgacttata cgctggtcgg
    11881 cattgttgcc ggactgacgg gcgcactgct gaccgtatgg ttgcagcagg cttgggtcgt
    11941 gttggcggca tcggctttga tggtcgtctt ggcactgtct atgttcgggc tgttcaacat
    12001 ccagcttccc aacgccgtgc agtcgtattt tcagaatcaa agcagcaggc tttcaggcgg
    12061 taaaatcgtt tccgtcttta ttatgggcat attgtccgcg ctgattgtcg ggccgtgcgt
    12121 cgccccgccg ctggcatttg ctttgggcta catcggtcag acgggcgatg cggttttagg
    12181 cggtttggca ctttacactt tggcgttggg caccggcgtt ccgctgattg ccatcggcac
    12241 gttcggcggg catatcctgc ctaaggcagg cgattggatg aatgccgtca aatacgcttt
    12301 cggctttatc ctgcttgccg tcgccgttta cctcgccacg ccgcacttgc cctattatct
    12361 cgtcgtcgcg ctgtacacgc tgctgatgct ggttcctgcc tgtatgctgc tggtcaacgg
    12421 acgcaggcag aaacgccgtc cgaaagctgt ggcattcgca ttgggcggca tattgctgat
    12481 aggcggcgcg tggttcggct ggcagggcgc aaacggcaaa acgaccgcgc tgcaccattt
    12541 cctgaccctc aatccgccgg ccgaagcagg caaatcttcg gaacacggaa aaatgtttgc
    12601 cgatactgcc gcgctgaagg cagcgatgga tacggcgttg aaagaacatc ccgacaaacc
    12661 cgtcgttttg gatttttatg ccgactggtg tatttcctgc aaagaaatgg cggcatacac
    12721 gctcaatcag ccggaagtgc atcaggcggt tgatatggaa cgctttttcc aaatcgacgt
    12781 aaccgccaac acgcccgaac atcaggcgtt gttgaaagaa tacggtctgt tcggaccgcc
    12841 gggcgtattt gtcgtccgct ccgacggcag ccgcagcgag ccgctgctgg gttttgtcaa
    12901 agcagacaag tttatcgagt ggtatgaaca aaaccgctga ttccgatgtt tcaaatgccg
    12961 tctgaagttc tcagacggag acctttgcaa aactcctttc cctcccgaca gccgaaaccc
//
LOCUS       DAXHKX010000034        12864 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHKX010000034 DAXHKX010000000
VERSION     DAXHKX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.034"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(54..362)
                     /locus_tag="ACFMWZ_000813"
     CDS             complement(54..362)
                     /locus_tag="ACFMWZ_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934802.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(346..648)
                     /locus_tag="ACFMWZ_000814"
     CDS             complement(346..648)
                     /locus_tag="ACFMWZ_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC5934803.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(722..3085)
                     /gene="pheT"
                     /locus_tag="ACFMWZ_000815"
     CDS             complement(722..3085)
                     /gene="pheT"
                     /locus_tag="ACFMWZ_000815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC5934804.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMLEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3121..3639)
                     /locus_tag="ACFMWZ_000816"
     CDS             complement(3121..3639)
                     /locus_tag="ACFMWZ_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934805.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3646..4710)
                     /locus_tag="ACFMWZ_000817"
     CDS             complement(3646..4710)
                     /locus_tag="ACFMWZ_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC5934806.1"
                     /translation="MIKSFELEKFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQATWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4887..5879)
                     /gene="pheS"
                     /locus_tag="ACFMWZ_000818"
     CDS             complement(4887..5879)
                     /gene="pheS"
                     /locus_tag="ACFMWZ_000818"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC5934807.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6225..6584)
                     /gene="rplT"
                     /locus_tag="ACFMWZ_000819"
     CDS             complement(6225..6584)
                     /gene="rplT"
                     /locus_tag="ACFMWZ_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC5934808.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6597..6794)
                     /gene="rpmI"
                     /locus_tag="ACFMWZ_000820"
     CDS             complement(6597..6794)
                     /gene="rpmI"
                     /locus_tag="ACFMWZ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC5934809.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6941..7462)
                     /gene="infC"
                     /locus_tag="ACFMWZ_000821"
     CDS             complement(6941..7462)
                     /gene="infC"
                     /locus_tag="ACFMWZ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC5934810.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7480..9393)
                     /gene="thrS"
                     /locus_tag="ACFMWZ_000822"
     CDS             complement(7480..9393)
                     /gene="thrS"
                     /locus_tag="ACFMWZ_000822"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC5934811.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKATVAGRVNGKL
                     VDACDPITEDSSVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMSEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWENYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEQRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENYAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RVELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9446..9522)
                     /locus_tag="ACFMWZ_000823"
     tRNA            complement(9446..9522)
                     /locus_tag="ACFMWZ_000823"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9486..9488),aa:Val,seq:gac)
     gene            9701..10816
                     /gene="tgt"
                     /locus_tag="ACFMWZ_000824"
     CDS             9701..10816
                     /gene="tgt"
                     /locus_tag="ACFMWZ_000824"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC5934812.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPMLPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQAQFHENRARGTD"
     gene            10826..11908
                     /gene="hemH"
                     /locus_tag="ACFMWZ_000825"
     CDS             10826..11908
                     /gene="hemH"
                     /locus_tag="ACFMWZ_000825"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC5934813.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETLEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12099..12500
                     /locus_tag="ACFMWZ_000826"
     CDS             12099..12500
                     /locus_tag="ACFMWZ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC5934814.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(12563..12853)
                     /locus_tag="ACFMWZ_000827"
     CDS             complement(12563..12853)
                     /locus_tag="ACFMWZ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934815.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3263 a         3397 c         2852 g         3352 t
ORIGIN      
        1 tattttttag cgtgtaaaac aaaaccggct gcatacctgc aaccggcctc aaatcagcac
       61 aattccttat ccaaatccgc caacaggtct ttcagaccgt ctccgatttc ctgaagcgta
      121 accggacggt tgccgtccga acccgattcc gcaccttctg catacggtgc aaacgtgctc
      181 tgcaatttca acagtttcac cacatccaaa cgggtgtagc gttcgccgcc gtaaccgaca
      241 accacaccgt gattatgctg ccattgcgca aaaccatagg ggctgatttg caacagtccg
      301 cacaactcgt ccagcgtgaa atagcgtttt gcgggaacct tcaaatcaac gttgtttgtc
      361 atagtagtgt tccaccatgc ttttgagttt ctggctggca tggaaagtta ccacgcggcg
      421 ggcggtaatc ggcacttcct cgccggtttt aggattacga cccgggcgtt gcggcttgtc
      481 gcgcaactgg aaatttccga aaccggaaat tttgatttct tcgccgcttg ccaaagtgct
      541 gcggatttct tcaaaaaaga gttcgacgat ttctttggca tcgtttttgg tgacgttgct
      601 gactttgtct accaaaatat cggccagttc tgctttagtg agagtcatat gattaccttc
      661 tgttgtaaca gttgtggatt attaccaatt tttatttaaa attcaagcga atatttattt
      721 tttaactgcg aagccgcgcc cctgccgccg ttgccgcgcc aatcagtttt ccgataagcg
      781 gctcgaccgc ctcatccgtc agcgtgtttt ccatatcctg caaaatcact ttgaccgcca
      841 cgctcttcat cccttcgggc agtcccgtgc cgcgatacac gtcaaacacg ctgatttcct
      901 gtaccaactt gtttgccgcg cctttcaaga caagcagcaa atcatcatgg ctcatagctt
      961 ccagcatcac aaacgccaaa tcgcggcgca ccggctggaa tttcgatacg gcccgatagc
     1021 gcgttttccc gcattccaac acggccgcca tatcgatttc aaataccagc ggcgcttgcg
     1081 gcaggtcgta tttttgcagc catttcggat gcagttcgcc gacaaagccg atgacgttgc
     1141 cgtctgaaac gatattggcg gcgcgtccgg gatgcagggc aggatgtccg gttttaacga
     1201 actcgaccgc tttgtttttc aacagatttt ccacatctgc cttaatgtcg taaaaatccg
     1261 cattgcgcgt tttcccgccc cattgttcgg gcattgccgc gccgtaccac aagccgccga
     1321 tgcgttcgtt ttggacaaac cggccgtctg aacctttgcc gaacacgcgg gcgatttcaa
     1381 acacgcgcac gcggttttgt ttgcggttca ggttgttttg caaaatttcc accaagccgc
     1441 cgatgagtgt ggaacgcatc acggcatatt gcgccgccag cgggttttgc aggcggatgg
     1501 ggtcggcgtt ggcggcaaaa tcttgttccc actgctcgtc aacgaaggca tagctgacca
     1561 cttcgcggta gccgcgcgcc gccatttcgt tgtaaacggc aaaacgcggg cggcgtgttt
     1621 cgggcagttc cagcattttc agacggcctg acgtgtaatc gtcggggatg ttttcatagc
     1681 cgtaaacgcg tccgatttct tcaatcaaat cagcctcaat ttcgatgtca aaacggaagc
     1741 tcggcgcggt aacgcggaag ccttccgccg ttttctcggg ctgcaggccc aagtgttgca
     1801 aaatggtttc cacctgttcg gcaggaatgt ccacgcccaa cacggttttc agacggccca
     1861 aacgcaatcc gacctgcttc gcttcgggca attcgccttg cgcttccacc atttcgcctg
     1921 ccgcaccgcc gcaaatctgc aacaccaatt cggtagcacg ctcaatggca tccgcctgca
     1981 aacggtaatc cacgccgcgc tcgaagcgga acgacgaatc cgaaccgaaa ccgtattggc
     2041 gcgatttgcc ggcgatgatt tcgggcgcaa accaagccgc ttccagcacg atattttgcg
     2101 tgccgtctga aaccgcgctc gccgcgccgc ccattaagcc cgccaaactc aacacgcctt
     2161 tttcgtccgc cacgaccagc gtgttttcag acagggaaac ggttttctcg ttcaggcatt
     2221 ccagcgtttc cccttcgcgc gcgcggcgga tgtgcaggct gccggaaagt ttgtcggcat
     2281 caaaaacgtg catcggctga ccgatttcca gcatcacata attgccgatg tccaccagcg
     2341 cggaaatact gcggatgccg ctgcgctcca aacgttgttt catccaatcc ggcgtagtag
     2401 cgcgcgcgtt cacgttttca atcacacggc tgataaagcg accgcaatcg gcaggcgcgt
     2461 caatctgcac gggctgtttt cgactgcccg tgatcggcgc ggtatggatt tcgggctgcc
     2521 tgaacgcgca ccccgtcaat gcggacactt cgcgcgcaat gcctttgatg ctcaagcagt
     2581 cggcgcggtt aggcgtaatt ttcaacgtaa acagcgtatc gtccaaatcc aagtattcgc
     2641 ggatattggt accgacgggc gcatcttcag gcagaatgtg caggccgttc acaccgtcgt
     2701 cgggcagacc gagttcgtcg gtggaacaca acatcccgtc cgacaccacg ccgcgcattt
     2761 tggtcggctt gattttgaaa ttgcccggca acactgcgcc cggcaacgaa cacggcactt
     2821 tgatgcccgc tttcacattc ggcgcaccgc acacaatctg caccaacccg cccgtacccg
     2881 catcaactcg ggtaacgttc aaacggtctg catccggatg tttttcaacg gatttcactt
     2941 cggcaatcac cacgcccgca aacgcaggcg cggcagtttc ggcctcttcc acttccaagc
     3001 cggacatcgt taacagatgt tccagcttat cggaggaaag ttcggtatcg gcttgggttt
     3061 tcagccatga ataggagaat tgcatgttat gttctcaatc aattaattaa ctttagattt
     3121 tcaagagcta gctaaacctt tttgaatggc taagtcccta ttaattactt ctacagaaat
     3181 gttccataaa gaaatcaacc gcaaagtatc tttcttatta aattcttgct ttattaattc
     3241 attcaattgc ccaagataat taagcggcac atccgggata accagtaata atttgatttc
     3301 tatgctaccc cattgcaaat ttgaaaaagc actaggcata attgtttctt tgatatcttc
     3361 tctgcctaat attcccaaaa tttcaacatt taatgcatta caccattttt gataaatctc
     3421 tgaaatatat tcatcaaaac gctcaggaga tctttcttga tttggaatac tttgttttgc
     3481 ctcaacaaat aacaaattat cttcttgcag atagataaac tctactgtct taataccttc
     3541 ttgccttctc ttccggatat atggttctat ataaaacagg ttatttttta ctagattaaa
     3601 agttatacct gattcagtga gatttattat ttcactcata aaaacctaac caatccatgc
     3661 tgtttccgga gataaaacac ctatttcttc tctgaacaat cgaatagact caccaataat
     3721 ttcattatcc ggcatgctat tttgtaaatt ttcctgcacc caagtagcct gttccttttc
     3781 tttgccaggc ataaaaacag gaatactttc actatgcttt ctagctaata aacacagctt
     3841 cttgataaca aaatatgaat gcgtactcat aaaaatctgt attcccaatt tggatttaga
     3901 taaggaaaac agaatttcta gaaattgatt aattgctttc ggatgtagat tagcttctgg
     3961 ctcatcaata aataagattg atccaggact caaaaaccga tttcctaata gagtgtctaa
     4021 tatcccaatc ttttttaccc cctctgctgt agaattgata gagaaagttt gtctatcttt
     4081 ttcatatacc catgccttct ttttatcatt gtagctaaca taaccgccaa acaatgcttc
     4141 tagatccttt ctagcttccg ctgcctcagg gtaattcctc ccttttgttg gtttaatatc
     4201 caatgctttt accaaatcaa tgtaagtagc atcaaaacca aatctctttt cttcctctga
     4261 ttttttaatt acatcaaata gtgatagaac ttctttgggt ggtaaaaaaa tagagttagc
     4321 gtttattctg ggagaaagtt cgtatagctc cggtttaatt agctttttgg tagttctacc
     4381 aaaagaaaac aaaactgaag aactatcttc taaagtcata cttaacttaa gaggccgttc
     4441 gtttggattt tcttttcctc ttgtaactaa atcaccaatt tcctctactt ggaacgtcca
     4501 atatagctta tctcgcaatt cctcagaaaa atcacgaata ttctttcctt tatgagcttc
     4561 ctcatgactt tttaaaacac tatataatgc ctttaaaata aaagttttcc ctttgctatt
     4621 ttctgcaata attaaattta ttttccctaa attatcagcc ttcaataagc taatcggtcc
     4681 gaatttttca agctcaaatg atttaatcat ataatccccc taatatatat caacaatatt
     4741 tgtacaagcc acattgtaaa aagacagcaa ccacagcacc atagtaaacg catagcaggc
     4801 ggataatgga gcgacatagc gtttcatgat ttagctttct gaggtaggca caggctgctt
     4861 ttttcatttt taggcagcct acacaatcat tttacaaact gcttcaaaaa attcaaatcg
     4921 ttatcaaaaa acaagcgcaa atcgttcacg ttgtaacgca gcatcgcgaa acggtcgaga
     4981 ccgataccga aagcgaaacc ggtgtatttt tcagaatcga tattcacatt tttcaacacg
     5041 ttgggatgca ccatgccgca gccgccgact tccagccatt tgccgttttc gcccataatg
     5101 tcgatttcgg cagacggttc ggtgaacggg aagaaagacg ggcggaaacg tacttgcaaa
     5161 tcatcgcgtt caaagaagcg acggataaaa tccgtgaaca ctgctttcaa atcggcaaaa
     5221 gttacgccct cttctaccca caaaccttcc gcctgatgga acataggcga gtgcgtggca
     5281 tcgctgtcca cacggtaaac gcggccgggg gcgataatgc ggatgggcgg ctctttttta
     5341 tcgagcatat agcggatttg aatcggggaa gtgtgcgtac gcaaaacatc gccgttttca
     5401 acgtaaaacg tatcctgcat cgcacgggca ggatggtttg cagggatgtt cagggcttgg
     5461 aaattgtgaa aatcgtcttc gatttcaggc ccgtccgcca cttcgaaacc cattccgtga
     5521 aagagttcga ccacacgttg caaggtcagg gttacgggat gcaggctgcc gccttcctga
     5581 gcgcgtccgg gcagggtaat atcgagggct tcggcggcaa gtcgggcttg cagcttgact
     5641 tcgttgaggg catcgcgttt ggcattaaaa gccgtctgaa accggttttt gcattcattg
     5701 atatgcgcac ctatggtttt gcgctcttca ggcgacattt gccccaaagt tttcagaagt
     5761 ccggtcaact cgccggtttt gccaagataa cgggctttga tttgttctag agcgttgaag
     5821 tcttgcgcag cttctactgc ggcaatgcct tctgcaacga tgcggtttac attttccata
     5881 atatcaagtc tgtcaattaa tgatatgcca cctgaaacaa cgaggccgcc tgtatagcct
     5941 gacatatcag actcttcaga cgaccttatt ttcttcacgg tcaatccatt ggataaattt
     6001 tctgtatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc gtactatctg
     6061 tactgtctgc ggcttcgtcg ccttgtcctg atttaaattt aatccactat attttaacgc
     6121 aaatttattt aaaaaaatag actttaaaga aagaaaatca atcacataga atttctgcaa
     6181 acaaagaaaa aaggaagccg cagcttcctt ttcaattttt tggattaagc agccaaagca
     6241 gctttggctt tttcaaccaa ttgtgcaaaa gcggctttat cgaacacggc caaatcagcc
     6301 aatactttgc ggtcgatttc aatagaggcg cgtttcagac cattcataaa tttgctgtaa
     6361 gacaacccgt tttcacgcgc acctgcattg atacggacaa tccacaattg acggaattgg
     6421 cgtttgcgtt ggcggcggtc acggtatgcg tattgaccgg ctttcattac cgcctgcttg
     6481 gcaacgcggt aaacgttttt acgacgaccg cgatagcctt tggctaacgc gaagattttt
     6541 tggtgacggg cacgggcggt aacaccgcgt tttacgcgtg gcatattcta aactccttaa
     6601 gcgtagggta acattttagc aacagaagcc aaatcgcgat cgtttaccat agaggtaccg
     6661 cgcagttggc gtttgttttt ggtggtcttt ttagtcaaga tgtggcgttt gaacgcatga
     6721 gcgcgtttca caccgccgtt acccagtact ttaaagcgtt ttttcgcgct agacttggtt
     6781 ttcattttag gcatgggaaa actccattcg ttatcggata aggcattagg gggttttaaa
     6841 acatcggttt caaacacttg aacccacaat accacggttt tttgccgcaa ttgaaaactt
     6901 actccgaagc ggcaatcgga gtaagcggag aattatagct ttattttttc ttcggcgcaa
     6961 tcatcattac catttggcga ccttccattt tgggaaagga ctcgatttgc gccacttcag
     7021 ccaaatcttc ttttacacgt tccaaaagtt gcgcgccgag ttgctggtga gccatttcac
     7081 gaccgcggaa acgcaatgtc actttgactt tatcgccgtc ggcaaggaag cggttgatgt
     7141 tgcgcatctt gatttgataa tcgccctcat ctgtacccgg acggaattta atttccttga
     7201 tttgtacctg cttttgattt ttcttggctt cgtcgcgctt cttggcttgt tggtatttgt
     7261 atttaccgta atccatcagt ttgcatacag gcggtttagc agttggggaa atctctacca
     7321 aatcgacatc ctgcccttcg gccatagcca aagcttcacg aactgaaacg acaccaagct
     7381 gttcgcctga ctcactgatt aaacgcactt ctttggcggt aatttcgccg ttgattcgtg
     7441 cttcgcgttc ttgagcgatg atgaatactc ctataaaaat taatgattga cgagggcatc
     7501 agtgatttct tgctgcaatt gcgcaatgaa atcatccaaa tccaaagaac ccaaatcttc
     7561 tgctttgcgg cgtaccgcca ctttgttttc ctgcttctcc ttatcgccga caacgatttg
     7621 ataagggaaa cggtattggc tgttgtcgcg gattttgtaa ccgatttttt cgttacgcaa
     7681 atccaattcg acacggaatc ctgccgcctg caatttggca gccacttccc gacaataatc
     7741 tgcctgattt tcggtaatat tcataattac caattgaacc ggagccagcc ataacgggaa
     7801 agagcctgca tagttctcaa tcagaatgcc gataaatcgc tccaaagaac ctaaaatggc
     7861 gcgatgcaac ataacaggtc gcgcacggtc attgttttcg gttacatatt ctgcatccaa
     7921 gcgttccggc aagacgaaat ccagttgtaa tgtaccgcat tgccaagaac gacccaaggc
     7981 atctctgaca tgatattcga ttttaggccc gtaaaacgca ccctcgcccg gcaattcgcc
     8041 ccattccacg ccgcaggcag tcaatgcctc gcgcaaaccc tgctctgcct tatcccacac
     8101 gtcatctgaa cctgcacgtt gttcagggcg aagagaaagc ttgacggata catcatggaa
     8161 accgaactgt ttgtagatgc gaaccaacaa ttcattgaac gcacgggcct cgctgacgat
     8221 ttgatcttcg gtacagaaga tatgcgcatc atcttgcaca aaaccgcgaa cacgcatcag
     8281 accatgcaat gcaccgcttg gttcattgcg gtggcaagaa ccgaattccg ccaaacgcat
     8341 cggcaaatcg cgatatgagc gcaaaccgtt attaaaaatt tgcacatgac ccggacagtt
     8401 catcggttta accgcatatt cgcgtttttc cgaactggtt acgaacatat tgtctttata
     8461 gttttcccag tggccggatt tttcccagaa agttttatcc ataatctgag gcgttttcac
     8521 ctctttataa ccggcagcat tcaactcttt acgcatatgc tgttcaatca cttgccacaa
     8581 agcccagcct ttaggatgcc aaaacaccat gcccggtgct tcgtcttgca aatggaacag
     8641 atccaattgc ttgccaagtt tgcggtggtc gcgcttttcg gcttcttcga tacgttgaat
     8701 ataggctttc aactcgtctt ttgtcgccca agccgtaccg tagatgcgtt gcagcatttc
     8761 attgttgctg tcgccgcgcc agtatgcgcc cgccagcttg gtcagtttga agtttttcag
     8821 gaaacgagta ttcggaacgt gcggtccgcg gcacatatcg acatattcct gatgatgata
     8881 catccccatc gcttccactt cggacatatc gtcaatcagg cgcagtttgt attcttcgcc
     8941 gcgctcttga aaaattttaa tcgcctccgc acgcggagtc atgattttga ccacatcata
     9001 gtcttgggca atcaattctt tcatacgcgc ttcaatggcg gcaacatctt ccggtgtaaa
     9061 cggtttttcc gtggcgatgt cgtaataaaa gccctcttca atgacggggc cgataaccat
     9121 ttttgcatta ggatagagtt gcttgacggc atgcccgaca agatgcgcgc aggaatggcg
     9181 gatgatttcg atgccttcct gatctttcgg agtaatgatt tggacgcttg aatcttcggt
     9241 aatcggatcg cacgcatcga ccaatttgcc gtttaccctg cctgccaccg tcgccttcgc
     9301 caaaccggca ccgatagacg cagcaatttg agccacggta acgggggatt cgtattggcg
     9361 gactgagccg tccggcaagg taatattcaa catcaaacgc tccgaaagat taaaaaataa
     9421 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
     9481 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
     9541 ttatcgggtc gttttaattt ttgcaagagg tatgtgcatt tctttatgca acattgtggc
     9601 aagatttggg gatgtcgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa
     9661 acatatttcg atccctgtcg cccaactgac attcaatctt atgcttaaat ttactttaca
     9721 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaatcgaaac
     9781 gccggtattt atgcctgtcg gtacttacgg ttcggttaag gcgatgaacc cgcaaaacct
     9841 gcacgatatc aaggcacaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     9901 tttggaagtt atcgggcaat tcggcggcct gcacggtttt atcggctggg acaaaccgat
     9961 tctgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    10021 agaaggctgt acgttcaaaa gcccgatcaa cggcgacaag ctgtttttat cgcctgaaat
    10081 ttctatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagttgg acgaatgcac
    10141 gccgggcgag gcgacgcgcg aacaggcgcg aaaatcgctg caaatgagcc tgcgctgggc
    10201 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcactgt tcggcatcgt
    10261 gcaaggcgcg atgtatgagg atttgcgcga agaatcgctg cgcggtttgg aacagtttga
    10321 ttttcccggc cttgccgtcg gcggcttgtc cgtcggcgaa cccaagcccg aaatgtaccg
    10381 tatgctgcgc gccgtcggtc cgatgctgcc cgaacacaag ccgcattacc tgatgggcgt
    10441 aggcacgccg gaagacctcg tgtacggcgt ggcacacggc atcgatatgt tcgactgcgt
    10501 gatgcccacc cgcaatgcgc gcaacggctg gctgttcacc cgttttggcg atttgaagat
    10561 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    10621 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcgccggcg aaatcttggg
    10681 cgcacagctc aacaccatcc acaacctgca tttctaccaa gtcattatgg cggaaatgcg
    10741 cgatgccata gaacaaggca aatttgccga ctggcaggca caattccacg aaaaccgcgc
    10801 acgcggcaca gactgattcc cggcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    10861 gctttaccct acccgcacta tttttcagaa accgatcatg cttccattct ttccagaacc
    10921 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    10981 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    11041 catcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgtt
    11101 gcgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgcccct
    11161 agaagtttac acagcacgcc aagctgccgc gcttgccgaa cgtatgcccg acctcatcgt
    11221 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    11281 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    11341 cggagcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    11401 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    11461 cctccgatat tgggcggaac acggacgtgg gaaaaaactg atgttgagtt ttcacggcgt
    11521 accgcagaag cactacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    11581 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    11641 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    11701 acagggggta accgagctgg acgtattctg cccgggcttt ttggcagact gtttggaaac
    11761 actggaagaa atcgccctga tggggcggga acagttttat gaagcaggcg gcaaaagcta
    11821 ccgctatatc ccctgcctca acgacaaccc cgactggata gacgcactcg tcgcacttgc
    11881 cgaagaaaac cttggcggct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    11941 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgctgga
    12001 caacacatca agtgcaacct tactaccgat tcggcaagaa aatctttcac ccgctacgca
    12061 aaatcaacaa caaggtttca aacattaagg aaacaccaat gaacacaacc cgactgccga
    12121 ccgccctcgt cttgggctgc ctctgcgccg ccgcttctgc cgccgacaac agcatcatga
    12181 caaaagggca aaaagtgtac gaatccaact gcgtcgcctg ccacggcaaa aagggcgaag
    12241 gccgcggaac catgtttccg ccgctctacc gctccgactt catcatgaaa aaaccgcagg
    12301 tgctgctgca cagcatggtc aaaggcatca acggtacaat caaagtcaac ggcaaaacct
    12361 acaacggatt catgcccgcc actgccatca gcgatgcgga cattgccgcc gtcgccactt
    12421 atatcatgaa cgcctttgac aacggcggcg gaagcgttac cgaaaaagac gtaaaacagg
    12481 caaaaggcaa aaaaaactaa acagacaaaa tgccgtctga aaccggcaat ccggcttcag
    12541 acggcattca aatcaaacct ttctaacgcg gtactttcag ccccatagac ccgatattgc
    12601 cgtccccgtc aacctccttc gcggtcagca taaagccgcc gatgtcaata cggccgcact
    12661 cgaccggctc ctgactgtcg gaacgcttat cgaaaagctc ggcaaggctc aaaccctctt
    12721 cgcccgcttc cagcctcaaa ccgtaagcaa gtgccaaatc acccgaacgt gctgccggca
    12781 aaacgacaaa ctcaccgaag aaatcaaaat actcacggac gctaataccc gtctcggtaa
    12841 agtatttcgc cattttatag tgaa
//
LOCUS       DAXHKX010000035        12273 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHKX010000035 DAXHKX010000000
VERSION     DAXHKX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.035"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(85..1188)
                     /gene="dnaN"
                     /locus_tag="ACFMWZ_000828"
     CDS             complement(85..1188)
                     /gene="dnaN"
                     /locus_tag="ACFMWZ_000828"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC5934816.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDVQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1268..2824)
                     /gene="dnaA"
                     /locus_tag="ACFMWZ_000829"
     CDS             complement(1268..2824)
                     /gene="dnaA"
                     /locus_tag="ACFMWZ_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC5934817.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMRNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAIGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3116..3250
                     /gene="rpmH"
                     /locus_tag="ACFMWZ_000830"
     CDS             3116..3250
                     /gene="rpmH"
                     /locus_tag="ACFMWZ_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC5934818.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3253..3618
                     /gene="rnpA"
                     /locus_tag="ACFMWZ_000831"
     CDS             3253..3618
                     /gene="rnpA"
                     /locus_tag="ACFMWZ_000831"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC5934819.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3683..3904
                     /gene="yidD"
                     /locus_tag="ACFMWZ_000832"
     CDS             3683..3904
                     /gene="yidD"
                     /locus_tag="ACFMWZ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC5934820.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4076..5713
                     /gene="yidC"
                     /locus_tag="ACFMWZ_000833"
     CDS             4076..5713
                     /gene="yidC"
                     /locus_tag="ACFMWZ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC5934821.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRSDPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5794..6504)
                     /locus_tag="ACFMWZ_000834"
     CDS             complement(5794..6504)
                     /locus_tag="ACFMWZ_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC5934822.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLPHEQQAVVGLTDFVVEAEKTARA
                     HLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQR
                     SRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(6566..7156)
                     /locus_tag="ACFMWZ_000835"
     CDS             complement(6566..7156)
                     /locus_tag="ACFMWZ_000835"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC5934823.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEGL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7304..7807
                     /locus_tag="ACFMWZ_000836"
     CDS             7304..7807
                     /locus_tag="ACFMWZ_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC5934824.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGSNGTPEKADRDKPNPFAV
                     LAGLKSN"
     gene            7841..8020
                     /gene="rpmF"
                     /locus_tag="ACFMWZ_000837"
     CDS             7841..8020
                     /gene="rpmF"
                     /locus_tag="ACFMWZ_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC5934825.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8195..8722
                     /locus_tag="ACFMWZ_000838"
     CDS             8195..8722
                     /locus_tag="ACFMWZ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC5934826.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            8810..9865
                     /gene="plsX"
                     /locus_tag="ACFMWZ_000839"
     CDS             8810..9865
                     /gene="plsX"
                     /locus_tag="ACFMWZ_000839"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC5934827.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            10001..10252
                     /locus_tag="ACFMWZ_000840"
     CDS             10001..10252
                     /locus_tag="ACFMWZ_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC5934828.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            10279..10695
                     /locus_tag="ACFMWZ_000841"
     CDS             10279..10695
                     /locus_tag="ACFMWZ_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934829.1"
                     /translation="MKTSTVVFGGFFMADNGERIQIPVLENPDIREINHFFSVSNFEK
                     KTGVLVFRIIPEPEFGNTELTVYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            10715..10918
                     /locus_tag="ACFMWZ_000842"
     CDS             10715..10918
                     /locus_tag="ACFMWZ_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934830.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            10945..11907
                     /locus_tag="ACFMWZ_000843"
     CDS             10945..11907
                     /locus_tag="ACFMWZ_000843"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC5934831.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            11954..>12273
                     /locus_tag="ACFMWZ_000844"
     CDS             11954..>12273
                     /locus_tag="ACFMWZ_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC5934832.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYSID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         3120 a         3639 c         2910 g         2604 t
ORIGIN      
        1 tttccgacag ccgaacaaaa ccccgaatcc gtctgcacgg ttcggggtat atctccaata
       61 cgggcatcgt gttccggaaa accgtcaaat ccgcatcggc atcacgatat atttgaaatt
      121 cggattgttc ggtacggtaa acaaggtcga acggttggca tcgccgaaag caagctgcac
      181 atcgtcggaa tgaatatttc gcaacacatc catcagataa ccgatattga aaccaacttc
      241 aagttcgccg ccctgatagg caatctcgat ttcttcacgc gcttcttcct gttcgttgtt
      301 gctgcacacg acgctcaaca ggccggattg caggaacagg cgcgcgccac ggaatttttc
      361 gttggcaaga atggacacac gttccagcgc gcccaaaagt tcggcacggg acaaaacgaa
      421 aatcttgtcg ttgtccaaag gaatcacgcg gttgaaatcg gggaatttgc cgtcgatgac
      481 cttgctgacg atggtcgtgc cgttgcattg gaaacgcacc tgtttgtcca gcagctcgat
      541 ttgaatcgga tcgtcggggt tgttcaacag tttgaacagt tccagcaccg ttttgcgcgg
      601 caaaatcact tcggcgcgcg gcaaatccgc atcaatcgcg caggctgcat aggcaaggcg
      661 gtgtccgtcg gtcgccacaa ggcgcaactg gctgccctca acctgcatca gcagaccgtt
      721 gagataatag cggatgtcct gcaccgccat gctgtactgc acttgcgaca gcatggtttt
      781 gaaacgctcc tgctccagcg agaaagtcgc gctgatgtcc tcgccgacat tcatcatcgg
      841 aaaatcggcg gcaggcaggg tctgcagggc aaaacgcgat ttgcccgctt tcagcgtcag
      901 acggctgtcg tcccaatcca gcgacaccag cgcaccggca ggcagtgccc gtaaaatatc
      961 ctgaaatttc ttggcattgg tggtgatgcg gaagtcgccc gcgccgccct cgggacccgc
     1021 agtgtcgatt tggatttcca aatcggttgc caagagtttg gtctgaccgc cttttccctc
     1081 aatcaggacg ttggacagga tgggcagggt gtggcggcgt tcgacgatgc cggtaacggc
     1141 ttgcagcggc ttgagcaggc tgtcgcgttc ggcttgtaaa atcaacattt tcgctcctta
     1201 aatcggttgg attttttacc cttcggacgg catgggcaat atcatgccgt ctgaaaggcg
     1261 cgttcggtca gttttgaatc agaatcagca gtttctcgta atcctgcgcc aactcggggt
     1321 cttcctcgcg cagtttcgcc actgccctga tgccgtgcat aacggtcgta tggtcgcgtc
     1381 cgccgaacga atcgccgata gacggcaggc tcaaagtggt cagttctttg gtcaggctca
     1441 tcgccacctg gcgcggacgg gcaatgtttc gtgtccgttt cttaccgagc acatcgctga
     1501 ttttgatgcg gtaatatttc gcaacggcat caataataat atcggcggtg atgactttgt
     1561 gcttctcggc aataatgtcc tgcaaagcgg tacgcgccaa atcgatgtcg atgacgggac
     1621 ggttcataaa gcggctgctc gctccgacgc ggttgaacgc gccttcaagc tcacgcacgt
     1681 tggaacggat cagattggca atgaacagcg cggcttcgtc ttcaatactg atgcccgccg
     1741 cttccgcctt tttctgcaaa atggcgatac gcatttccaa ttcgggcggc tcgagttcca
     1801 aagtcagtcc ccatgaaaaa cgggatttga ggcggtcgtc cataccttcg attttggcgg
     1861 gtaaaacatc gcaagtgagg atgagctgtt ttttctcgtt atggaaatga ttgtacagat
     1921 agaagaattc ctccatcgta cggtctttgc ctttgatgaa ctggatgtcg tcgataatca
     1981 gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta agtgttgttg cgcacggctt
     2041 tcatgaagct gcggatataa tcgtccgaat gcatatagcg tactttcgcg tcgggacggt
     2101 ttttcaaaag ctcgttgccg atggcctgaa ccaagtgggt tttacccaaa cccgtactgc
     2161 catagaggaa aaacgggttg tagctctgcc ccgggctttc cgcaatcgcc tgcgcggcag
     2221 ccgccgcaag gcggttgccc ttgccttcga ccaatgtatc gaacgtataa tccggcgaca
     2281 gattggtctg ctcgtaacgc gcctcttccg catcgcgctg cgcatccgtc cgcgctttcg
     2341 caagcacctc cgattccgga cgggaagcag gttcggcagt atggcgcggc tcgtgcggca
     2401 ggtttctcat acgttccgcc aaaatatccg ccgccgtttt cgacgcagcg ggtttgacag
     2461 gctcttcaga cggcagctca tccaacagaa cctcctgcac aggcatctcc tccgaccccg
     2521 cgtgcaagga cggctcggca ggttcgacag caccttcaac cgccgccatc tcataacgca
     2581 cgccttctcc cggtttgaat acgaaggcgg aacggccggc agccaactct tccctcaccg
     2641 cttctatttt tccggcaaac tggctcttga gcatattgca ggcaaactgg ttcttgccgt
     2701 acaccaccca tacgcccccc tcctcaccga cggtaagggg cgcaatccat tgcgcaaact
     2761 gcccttgagg caacatatcg tgaagacggc ggaggcacag cggccaaaac tctgctaatg
     2821 tcatggatag gctcgaatcg gtaaaaatga aatcgaaaac aaagaaaata taatattttc
     2881 aaaaagaaaa caaatctgtt gaacgcacat cggttcaaaa cgcgactgcc cgattatacc
     2941 gactcacgaa tattttatcc acaacccgtg caaaaattta tccacagaaa ggcggcggaa
     3001 atccgcaggc aatcgggcaa tcttcctgca aagtttctat attgattgac aaaagcggca
     3061 aattggagtg taattcacgg tttaattatc tacccattct attttaggaa acatcatgaa
     3121 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
     3181 caaaacacgc ggcggccgcg cagtattggc cgcacgccgt gccaaaggcc gcaaacgcct
     3241 ggcggtgtaa ttttggacta ccgcttcgga aggcagtacc gcttgttgaa aacggatgat
     3301 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgt
     3361 tcaaacggca acgggctgga tcatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
     3421 aaacgcgcca acgaacgaaa ttatatgaag cgcgtcatcc gcgactggtt cagattgaac
     3481 aaaaaccggc tgccgccgca ggatttcgtc gtgcgcgtcc gccgtaaatt cgacagggct
     3541 accgcaaaac aggcaagggc ggaactggca caactcatgt tcggcaaccc cgcaaccgga
     3601 tgcaggaaac aggcatgatc agaacggtac tctgcaggca aggttcagac ggcaacgggt
     3661 ttcccataca aggaacatcc cgatgaactt cctattgtcc aaactcctgc tgggactgat
     3721 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgtt atacgccgac
     3781 ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc gcattcaaag gcggccggct
     3841 cgccatcaag cgcattgcac gctgccaccc tttcggcgga cacggacacg accccgtccc
     3901 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
     3961 aatgccgtct gaaccttcgg gctggcggca cgaccgtcgg gtatgaagcc cgcccttatt
     4021 ccccgtctat cggaatacgc aacctgcggc atttccgacc attcaggaaa ctcttatgga
     4081 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
     4141 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
     4201 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
     4261 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
     4321 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
     4381 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
     4441 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
     4501 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
     4561 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
     4621 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggctact ttacccactc
     4681 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttctccga
     4741 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
     4801 cggctggctc ggcatgattg aacaccactt catgtccacc tggatcctcc aacccaaagg
     4861 cggacaaagc gtttgcgccg caggcgactg ccgtatcgac atcaaacgtc gcaacgacaa
     4921 gctgtacagc accagcgtca gcgtgccttt agccgctatc caaaacggcg cgaaatccga
     4981 agcctccatc aacctctacg ccggcccgca gaccacatcc gtcatcgcaa acatcgccga
     5041 caacctgcaa ctggcaaaag actacggcaa agtacactgg ttcgcttccc cactcttctg
     5101 gctcctgaac caactgcaca acatcatcgg caactggggc tgggcaatcg tcgttttgac
     5161 catcatcgtc aaagccgtac tgtatccatt gaccaacgcc tcttaccgct ctatggcaaa
     5221 aatgcgtgcc gccgcaccta aattgcaagc catcaaagag aaatacggcg acgaccgtat
     5281 ggcgcaacaa caagcgatga tgcagcttta caaagacgaa aaaatcaacc cgctgggcgg
     5341 ctgtctgcct atgttgttgc aaatccccgt cttcatcggc ttgtactggg cattgttcgc
     5401 ctccgtagaa ttgcgtcagg caccttggct gggctggatt accgacctca gccgttccga
     5461 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cctatctgaa
     5521 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
     5581 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
     5641 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgca cccaaggcga
     5701 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
     5761 tttattgttc accccctatc ggggcggaaa tcttcaacct gcatacatca caaaaatcgt
     5821 cgggcgtttt ttcagattgg gcatttcctt catcctgcgc caatccgcaa ccgttttact
     5881 gaccaccgac tgagtcggca acgtcaaatc cgtagccgtg cacaagcgcg tttcaggatg
     5941 caggttttcc accgcatccg ccaacaacac atcgttgcga tacggcgttt cgataaacag
     6001 ctgcgtttca ttctgctggc gcgaacgttg ctccaaagcc ttcaaactct gaatccgctc
     6061 gtttttttca gacggcagat aacccttaaa cgcaaaactc tgcccgttcg cacccgaagc
     6121 catcaaagcc agcagcaggc tggaaggccc aaccagcgga cgcacttcaa aaccgtgttt
     6181 atgcgccaaa gccaccagat tcgcacccgg atcggcaaca gccgggcaac ccgcctcgct
     6241 gacaatgccc atactgcgcc cttcctgcaa aggtttcagc aattccggca aagtcttcaa
     6301 atccgtgtgt tcgttcagcg tttgcaaatt cagttcgcgg ataggcattg tcacgcccaa
     6361 atgtttcaaa tgcgcgcgcg ccgttttttc cgcttccacg acaaaatccg tcagcccgac
     6421 aaccgcctgt tgttcatgcg gcaacaggca cggcgtatcg ggcgtaccca aaggcgtagg
     6481 aatcaaatac aaaacaggag acatcattcc ctcactcatc ggttaaaaat gccgtctgag
     6541 cctttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaagaaat
     6601 cgaccagacg gaaaaccggc aaaccgatta aagcattcgg atcgctgctt tcaatccgtt
     6661 cgatcagcag cgcgcccaag ccctcgctct tcaacgcaca cgagcaataa accgcatcag
     6721 gctcgcgctc caaatagcgg aggatgtgca gctcgtccaa cttcctcatc acgaccaccg
     6781 tcttatcgat atgccgctgc atcctgcccg taaccgtatt tagcagaacg accgcgctgt
     6841 aaaactcaat ctccctgccg ctcaaatgca tcagcatctt ttgcgcgttg gcaaggttca
     6901 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
     6961 cctcggggaa acgtccggcc aacgacctcg ccttaccctc ggcaaggcgc aatgccgtct
     7021 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
     7081 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
     7141 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcggtaaa
     7201 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
     7261 aaatattgac gttagaacat ctaaattata tcatatctcg tttatgtcag accctaattt
     7321 gattgacccg gaaattttcg ccgccgaagg gcagaatctg caaggcagtt ttctgctgga
     7381 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
     7441 gtttacactg accggcggtc gcgatcggct gcaacgcctg ttcctcgacc tgaacgtcaa
     7501 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
     7561 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
     7621 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
     7681 ccaaatcctg atgtcgctcc ccttctctcc ccggcacgaa gactgcggca gcaacgggac
     7741 accggaaaaa gccgatcggg acaaaccaaa ccccttcgcc gttttggcag gtttgaaaag
     7801 caattgatta ggacacagtt tatttatcca ggagcttgaa atggccgttc aacaaaacaa
     7861 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcccgcact
     7921 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
     7981 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
     8041 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
     8101 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
     8161 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
     8221 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa
     8281 atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta tgctgcgctg
     8341 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acaacgaagg
     8401 acaggttaca gaagaagtca aaaaagtcca atggctcgac cccgttcccg aagccctgcg
     8461 gggcaaaaac gtccttgttg tcgatgaagt ggacgacagc cgcgtaacca tggaattttg
     8521 cctgacagag ctgcttaaag aagacttcgg caccatcggc gtcgccgtac tgcacgaaaa
     8581 aatcaaagcc aaagcaggta aaatccccga aggcattccc tatttcagcg gcatcaccgt
     8641 agaagactgg tggatcaact atccatggga cgcactcgac atcgacgaac acaaccgcct
     8701 tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc gatgccgtct
     8761 gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaacccta tgattacact
     8821 tgccgtagat gccatgggcg gcgaccaagg acttgccgtt accgtacccg gcgcaaccgc
     8881 attcctccaa gcacaccccg atgtccgcct gattatgacc ggcgacgaaa cgcaactgcg
     8941 ccaagccctg accgcggcag gcgcaccgat ggaacgcatc gacatctgcc acaccgcaca
     9001 aatcgtcggc atggacgaag cgccgcaatc cgccctaaag aacaaaaaag actcctccat
     9061 gcgcgttgcc atcaaccaag ttaaagaagg caaagcccaa gccgccgtat ccgcaggcaa
     9121 cacgggcgca ctcatggcaa ccgcacgttt cgtcctcaag accatacccg gtatcgaacg
     9181 ccccgccatc gccaaattcc tcccgtccga caccgaccat gttaccctcg ccctcgacct
     9241 tggcgcgaac gtcgactgca cgcccgaaca gctcgcccaa tttgccgtta tcggcagcga
     9301 actcgtccac gcactccatc ctcaaaaagg acagccgcgc gtcgggctgg tcaacgtcgg
     9361 cacggaagac atcaaaggta cggacaccgt caaacaaacc tacaaactgc tgcaaaacag
     9421 caaactcaac tttatcggca acatcgaaag caacggcatc ctgtacggcg aagcagatgt
     9481 cgtcgtcgcc gacggctttg tcggcaacgt catgctcaaa accatcgaag gcgcggtcaa
     9541 attcatgagc ggagccatcc gccgcgaatt ccaaagcaac ctgttcaaca aacttgccgc
     9601 cgttgccgcc ctacccgccc tcaaagggct gaaaaacaaa ctcgatccgc gcaaattcaa
     9661 cggggccatc ctgctcgggc tgcgcggcat cgtcatcaaa agccacggcg gcacagacga
     9721 aaccggtttc cgctacgcac tcgaagaagc ctaccacgaa gccaagtccg ccggcctttc
     9781 caaaatcgaa caaggcgttg ccgaacaact cgccgcactc gaaactgcca aagccgtcca
     9841 aaacgaaaat gccgacggtc tgtaacacac acgatgccgt ctgaacgccc ccgccccttt
     9901 cagacggcat ccgcccgcac caaacctgcg ggcgcggacg gcgatgcgcc tgtccggcac
     9961 ttcccaaata cttccctgta aaataaggag tatttgaaaa atgaagacat tagaaaaacg
    10021 gatgaaagct ctagacaaac agatgatgaa gttcggaaaa tcccttgaag gcaggcttga
    10081 tgcccgtctg attgaatccg cattggatta tattcattat tcggaacgtt ttttggcttt
    10141 tgaaatcctg tgtacttata tcgaagattt cgatgtccgg ctgacggaac aagaatcccg
    10201 ggaaatttct tttatcaaca aggaatttga gatagaaagc acgtccgatt aaccaataaa
    10261 gccaatgggt tgataaacat gaaaacatcg acggtcgttt ttggcggatt ttttatggca
    10321 gacaacggag agcgaatcca aatccccgtt ttggaaaatc ctgacattag ggaaatcaat
    10381 cacttttttt ccgtatcaaa ttttgagaaa aaaaccggcg tccttgtttt cagaatcatc
    10441 cccgagccgg aatttggcaa taccgaatta actgtctatt ttgaaaaagg atattatttg
    10501 ccaatgattc aaacaatttt agaaggcggg gatattgaag tcaaaaacct taaaacggaa
    10561 aattatagtg gaaatacaat ggaaatttgg ggagattcct accctattga gcatatctct
    10621 aaaaatattt ctacaattca aaatataatt tctgaattta tgatacaaaa acaaacaccg
    10681 gcaccaataa tttgatggaa aaccaacaat caaaatgccg tctgaagcat gtttggtttt
    10741 cagacggcat ttccttcgct taaaacagcg tatcggcaac cccgccctgc ctgtccacgg
    10801 caatctgcat ctgaaaacca tctgtatccc aaaccacacc cccatccctg tttccgtcat
    10861 ttgcaccctg cccgtattgg gcaatcatcg gtttttcgct tacaatagcc gaatctgaac
    10921 caactatcca aaaaggccgt tcccatgcag tatgccaaaa tttccggcac gggcagctat
    10981 cttcccgcca accgcgtcag caatgacgac cttgcccaaa aagtggatac ctcggacgag
    11041 tggattaccg cgcgtacggg catcaagttc cgccatattg cagccgaaaa tgagaaaacc
    11101 agcgatcttg ccgccgaagc agcccgccgc gcactggatg cagccggatt agacagcggc
    11161 gaaatcgatt tgattatcgt ggcgacggca acgccggata tgcagtttcc ttctaccgcc
    11221 accatcgtgc agcaaaaatt gggcatcacc aacggctgtc ccgcgtttga cgtacaggca
    11281 gtgtgcgcag gctttatgta cgcgctgacc actgcaaacg cctacatcaa aagcggcatg
    11341 gcaaaaaacg cgctggtcat cggcgcggaa accttcagtc gcattgtaga ctggaacgac
    11401 cgcacaacct gcgtattgtt cggcgacggc gcgggcgcgg tggttttgag cgcggcggac
    11461 aaaccgggca tcatccacag caaactcaaa gccgacggca attatctgaa actcttaaac
    11521 gtccccgggc aaatcgcctg cggcaaagtt tccggttcgc cgtacatttc gatggacggt
    11581 cccggcgtgt tcaagtttgc cgtcaaaatg ctgtccaaaa tcgccgatga cgttatcgaa
    11641 gaagccggtt acaccgccgc tcaaatcgac tggatcgtgc cgcatcaggc aaaccgccgc
    11701 attatcgaat cgaccgcgaa acatttgggg ctgagtatgg acaaagtcgt cctgaccgtc
    11761 caagaccacg gcaacacgtc cgccgcctcg attccgctgg ctttggatac aggcatccgc
    11821 agcggacaaa tcaaacgcgg tcaaaacctg ctgctcgaag gcatcggcgg tggtttcgcg
    11881 tggggcgcgg tgctgttgca atattgaacc cgatgccgtc tgaagcaggt tttcagacgg
    11941 catttcccat atcatgaagc ggcaggcttt cttcaaacca attgcgtgtg cggcatttct
    12001 gtctgccgtt tcgctgcgcc tccccgtatt gggcgcgtgt tacgcaatat tgtccctcta
    12061 tgcgtttgca ctttacagca tcgacaaacg gcgtgccgtg cggggaaaac gccgcattcc
    12121 cgaacaccgc ctgctgctgc ctgccttgtt cggcggttgg gcgggcgcat acttgggcag
    12181 caggatattc aggcataaaa cggcgaaaaa gcgttttgtt gtgctgttcc gtctgactgt
    12241 ttcaggtaat gtcctggcga ccctcatcct gat
//
LOCUS       DAXHKX010000036        12244 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHKX010000036 DAXHKX010000000
VERSION     DAXHKX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.036"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="ACFMWZ_000845"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="ACFMWZ_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC5934833.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="ACFMWZ_000846"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="ACFMWZ_000846"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC5934834.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="ACFMWZ_000847"
     CDS             1963..2286
                     /locus_tag="ACFMWZ_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC5934835.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAVLLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2333..2809
                     /gene="ribH"
                     /locus_tag="ACFMWZ_000848"
     CDS             2333..2809
                     /gene="ribH"
                     /locus_tag="ACFMWZ_000848"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC5934836.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2887..3312
                     /gene="nusB"
                     /locus_tag="ACFMWZ_000849"
     CDS             2887..3312
                     /gene="nusB"
                     /locus_tag="ACFMWZ_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC5934837.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3676..4710)
                     /gene="pyrC"
                     /locus_tag="ACFMWZ_000850"
     CDS             complement(3676..4710)
                     /gene="pyrC"
                     /locus_tag="ACFMWZ_000850"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC5934838.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTAHQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIERVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWMVQY"
     gene            complement(4926..5072)
                     /locus_tag="ACFMWZ_000851"
     CDS             complement(4926..5072)
                     /locus_tag="ACFMWZ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934839.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            complement(5073..5297)
                     /locus_tag="ACFMWZ_000852"
     CDS             complement(5073..5297)
                     /locus_tag="ACFMWZ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC5934840.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLVTDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5406..6017
                     /locus_tag="ACFMWZ_000853"
     CDS             5406..6017
                     /locus_tag="ACFMWZ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC5934841.1"
                     /translation="MRRAILLILALTAGTSLATSFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGDVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(6077..6949)
                     /gene="accD"
                     /locus_tag="ACFMWZ_000854"
     CDS             complement(6077..6949)
                     /gene="accD"
                     /locus_tag="ACFMWZ_000854"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC5934842.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6986..7771)
                     /gene="trpA"
                     /locus_tag="ACFMWZ_000855"
     CDS             complement(6986..7771)
                     /gene="trpA"
                     /locus_tag="ACFMWZ_000855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC5934843.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            complement(8198..8290)
                     /locus_tag="ACFMWZ_000856"
     tRNA            complement(8198..8290)
                     /locus_tag="ACFMWZ_000856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8254..8256),aa:Ser,seq:gct)
     gene            complement(8447..8647)
                     /locus_tag="ACFMWZ_000857"
                     /pseudo
     CDS             complement(8447..8647)
                     /locus_tag="ACFMWZ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(8749..9066)
                     /locus_tag="ACFMWZ_000858"
                     /pseudo
     CDS             complement(8749..9066)
                     /locus_tag="ACFMWZ_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(9089..9850)
                     /gene="kdsB"
                     /locus_tag="ACFMWZ_000859"
     CDS             complement(9089..9850)
                     /gene="kdsB"
                     /locus_tag="ACFMWZ_000859"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC5934844.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSSLETIESLEQLRVL
                     WHGYSIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            complement(9847..10029)
                     /locus_tag="ACFMWZ_000860"
     CDS             complement(9847..10029)
                     /locus_tag="ACFMWZ_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC5934845.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            complement(10098..10679)
                     /locus_tag="ACFMWZ_000861"
     CDS             complement(10098..10679)
                     /locus_tag="ACFMWZ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC5934846.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            complement(10885..11919)
                     /gene="lpxK"
                     /locus_tag="ACFMWZ_000862"
     CDS             complement(10885..11919)
                     /gene="lpxK"
                     /locus_tag="ACFMWZ_000862"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC5934847.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPSADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            complement(12027..>12244)
                     /locus_tag="ACFMWZ_000863"
     CDS             complement(12027..>12244)
                     /locus_tag="ACFMWZ_000863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934848.1"
                     /translation="RYWKMKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHDS
                     FGQFLFGCSAEATRTAPISVKRSCLFT"
BASE COUNT         2953 a         3562 c         2917 g         2812 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt gagcagctcc aacaggttgg caatccgcaa tccgtgtttc gcactgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacttgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttgtcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acacggtcgg catcggtaaa gcgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccaagcgcct cggtaacgtt ttgattcagc ctgtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaagccgg gcgtatcgac aaacacgaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtgatgc
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacgaccga
      961 caatcgccac gaagccgcaa cggtatccgc cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat attcgctttc atatccacat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgcc
     1141 tcctgttccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaaccacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aagcaaagcg gcgtgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttggaaatc ggcacaccgg acgcggtcgg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc ggcatcgaag ctgaccgccg caaacatcgc ctccatcgcg
     1441 tccgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 agaccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgataa ctcgccctcg gtcaacttcg gaaacgtgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatca ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtctct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcacgaa
     1921 tcgtggtaaa atccgtaagt tttcccccaa cgtattcccg taatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgtatc gccaccaaca ccgtcgccaa acaaaaccgg
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgatcccg acaacttcag catcgtcagc
     2161 ctcgccgaca tcgccaaaac caaccctgaa accgccgtac tgctttccca acctgaaggc
     2221 agcgcgtggg aattggcaga agacggcaca ttccaaaccg tcgccgactg gcaaccgcaa
     2281 gactgaaacc ttcagacggc attccgaaac cccgaatacc aaaggacacc caatgaacac
     2341 catcgccccc aaccttgacg gcaaacacct ccgcatcggc atcgtacagg cacgtttcac
     2401 caacgaaatc ggcagcgaaa tgctcaaagt ctgctgccgc accctccaag aattgggcgt
     2461 ggcagacgaa aacattaccg tcgccaccgt acccggcgcg cttgaaatcc ccatcgcgct
     2521 gatgaacttt gcctcttccg aaaaatttga cgcactgatt gccatcggcg tcgtcatccg
     2581 tggcgaaacc taccatttcg agctggtttc caacgaatcc ggagcaggcg tcagccgcgt
     2641 cgcactcgac tacaacatcc cgattgccaa tgccgtcctg accaccgaaa acgacgcgca
     2701 ggcaatcgaa cggattgaag aaaaagcctc ggatgccgcc aaagtcgccg tagaatgcgc
     2761 caacctcgtc aacctgctgc tcgaagaaca gtttgaagac gaagaataac aaacagggcg
     2821 tacaccgatg cgccctttcc gtttcagacg gcataccgtg ccgcccaacc gtttcaagga
     2881 aaaatcatga aaacagcccg ccgccgttcc cgcgagcttg ccgtacaagc cgtttaccaa
     2941 tcccttatca accgcaccgc cgcgcccgaa attgctaaaa acatccgcga aatgtccgac
     3001 tttgccaagg cagacgaaga attgttcaac aaacttttct tcggcacgca aaccaatgcg
     3061 gcagagtaca tccgacaaat ccgcccactg ctcgaccgcg acgaaaaaga cctcaacccc
     3121 atcgaacgcg ccgtcctgct gaccgcctgc cacgagctgt ccgccatgcc cgaaacgccc
     3181 taccccgtca ttatcaacga agccatcgaa gtcaccaaaa ccttcggcgg cacggacggg
     3241 cacaaattcg tcaacggcat cctcgacaaa ctcgccgccc aaatccgccc cgacgagccc
     3301 aaacgccgtt gatgcatcac aaatttaaag aaagttctat gtccttattt ctgctttctt
     3361 tgtttcgtca ttcccacgca ggtgggaatt tggttttttg aattttgggt atttcaaata
     3421 aattctgaaa cttcaatctc gtcattcccg cgcaggcggg aatccggttt tttgagttgc
     3481 tgccgttttt gataaactct agtcgcatcg aatttccaga ttcctgcctt cgtgggaatg
     3541 gcgaaatttg aaaattgtgg gaatttatcg gaaataaccg aaacgtaaca aactcaaacg
     3601 ccgtagacgt atcaatcagt cagcaacaca aatgccgtct gaaagccctt cagacggcat
     3661 tttgtttcca gcctatcaat actgtaccat ccagccgatt tcgccgcccg cgcgcatcgg
     3721 gacaagttcg ccatcgccgt agggaacact tgcggggacg gtttggcttt gtttgacgag
     3781 ggtgattgtg tcggtatttt cgggaatgcc gtagaacctt gcgccgtttt tcgaggcaaa
     3841 ggcttcgagt ttgtccaacg cgcctgcttt ttcaaatact tcggcgtaaa gctcgacggc
     3901 ggtcatcgcg ctgaacatac cggcgcagcc gcaggcgttt tctttggcgg acttggcgtg
     3961 cggtgcggaa tcggtgccga ggaagaattt gtgcgccttc tcgccggtaa cggcagcaac
     4021 caatgcctga cggtgggttt cgcgtttgag tacgggcagg cagaaatgat gggggcgcac
     4081 gccgccgacc aagaggtcgt tgcggttaag cagcagatgt tgcggggtca cggaggcggc
     4141 aacgttgtcg cctgcttcca aaaccaagcg ggcggcttcg gcggtggtga tgtgttcgaa
     4201 cacgacttta agattcggca cttgcgccaa aacgggtttc atcacgcgct cgataaaggc
     4261 ggcttcgcgg tcgaaaatat cgatttcggg gtcggttact tcgccgtgaa cgaggaacag
     4321 gatgtcctgt tttgccattt cttccaacac ggggatgagc ttgaataggt cggttacgcc
     4381 ggaatcggaa ttggtcgtcg cgccggcagg gtagagtttg aaggcgacga tgccggcggc
     4441 tttggcttcg cgcacaagtt ccggcgtggc ttgatcggtc aaataaagcg tcatcagcgg
     4501 ctcaaacgca ctgccttcgg gcaacgccgc cataatgcgc gctttgtagg caagcgcgtc
     4561 ggcaacgctg acgacgggcg gtttgaggtt gggcataatg acggcgcgcc ccatttggtg
     4621 ggcggtatag ggtgcaacag ctttgagcgc gtcgccgtcg cgcaggtgca ggtgcatatc
     4681 gtcggggcgg ataatggtca gggtttgcat ggttgttcct ttttaggttt tggctgcgtt
     4741 tgaatacccg gtcgattcca tccgtttgcc gggggaaagc cggttattcc gtccggtcgg
     4801 aaaccggttc cattggatga aaggcatttt gtccgactga ttgggttgca tcaacggaat
     4861 cttcaaacac agcctttaga ttttgattgt gcttgggttt tgcttctttg ccgacggcaa
     4921 ggcggttaac agccattccg gaacaaatcg gcggcgatac gcccgaaatg ccgacactgc
     4981 acgccggacg gtttgttttt gaaacggcac gcggcacata cgatattcaa aaacaacgcc
     5041 gtcgggcggg acatccgcat ttcagacggc atttatttgt gttggacgac cagcgtgaac
     5101 acgccgtcct gttcggaagc atccaacagc acatgaccgg tttggcggca aaaagcctca
     5161 aaatcccccg gtgcgccgcc gtcggttacc agaacggtca acacgtcgcc ctgctgcatt
     5221 tgcgccaaag cctttttcgc ccgcaaaatc ggcaggggac atttcaatcc ggttacgtct
     5281 aaagtttcgc tgttcatcgt ttgtaccgtt taaaaaatgg gaatcggggg gagattatac
     5341 cccagttgct tgcagccccc tttaccaacc cataaaataa cgccgttttc aatttggaga
     5401 gatctatgcg ccgcgccatc cttctgattc tggccctgac tgccggcacg tcccttgcca
     5461 ccagcttcaa ccaaaaagac acgccgatca acacccgcta ccgcgaaacg cccgaagaag
     5521 ccgccgcccg cgaatttaaa gaacataccg ccaaactgcc gccgctgccc gatacgcact
     5581 cggacggctg gttcgacatt tatgtggatg aaaattacgg caaacagccg aaaatcctgc
     5641 tcgacagcct gcaaattatg cccgcgcccg acggcagcat ccgctacatt ctcaatatcc
     5701 gctccgacaa aggctacgac aacctgactg ccgaaggcat attctgcgcc cgcttctcca
     5761 tcaggttcgg caacggcaaa ccttcctcct ataaagtgtt cggctacggc gacacggtca
     5821 accgccgctg gatacagccc cgaaacgccg aatggaaacc gataggcggt acattaagcc
     5881 gcaacgacgc actgcgcacc gtcctgtatc aggcattctg cgagggcgat gtacccgccg
     5941 acacgaaagg tttggtacaa cgcctgaaag aacgtgccgg acgctatgcc ccgtcgataa
     6001 agccgcacaa caaataacca gacacaaaaa taccgtctga aacatcggtt tcagacggta
     6061 ttcgattcat cagccatcaa gcggcggaaa ctttgtcctg acggcacaac agcgtaatca
     6121 aatcactgat gcgccgcttc atctcacgcc ggtcaacaat ctggtcgatt gcgcctttct
     6181 ccagtaaaaa ctcagcacgc tggaagcctt ccggcaaggt ttcccgtaca gtctgttcaa
     6241 taacgcgcgg tcccgcaaaa ccgattaggg cattgggttc agccaatacg acatcaccca
     6301 aaaatgcgaa gctggcggat acgccgccca tagtcggatc ggtcaacacc gatataaacg
     6361 gcaggcgttt ttccgtcagc aaatgcagcg cggcactggt tttcgtcatc tgcatcaatg
     6421 agttcacacc ctcctgcata cgcgcaccgc cggaagccgc cacacagacg aacggacaat
     6481 tgtcggcaac cgcccggcgg acgccctgta cgaatcgttc gcccacaacc gaacccatcg
     6541 aaccgccgat aaagcggaac tcaaacgcag caaccacaac gggcagaccg ttcatcttgc
     6601 ctttcattac caccaaagca tcatcttccc cggtcagctt gcgtgccgca ctcaaacgat
     6661 ccggatattt tttgccgtct ttaaacttca aaggatctgt cggtttgaca tttccggcaa
     6721 cctcctctct gccatcctca tccaaaagca gattcaggcg ttgtcgtgcc gacaacggat
     6781 tgtggtggtt gcatttcgga caaacctgat tgttctgctg caactcggtc gaataaacgg
     6841 ttgccgaaca agacggacat ttgtgccaca aaccctcggg aacattggaa gaaccgtctt
     6901 tgctgcggtt tttgattttg ggtggcagga ttttatctaa ccagctcatg gacaactcct
     6961 aaaatattta gaatgcgtga cagaatcagc gcacggcatc cttcaactct ttgaccaaag
     7021 caccgacggc cgcagcctcg ttgcccgcat tgttttcaat ctctttcaca atccggctgc
     7081 cgacaatgac cgcgtcggca acccggccga ttttgcgcgc gctttccgca ttgctgatgc
     7141 cgaaaccgac accgatggga atatcgatat actgatgcaa atactctatt ttacgcgaaa
     7201 cctcatccgt atccaaactt gccgcgcccg ttacgccctt gagcgaaaca taatagacaa
     7261 atccgcctgc cagctcggca atggttttaa tacggtcttc cgtcgtcgtc ggcgcaatca
     7321 ggaaaataca gtcgaccccg ttatccttca gctcgcgata gagcggatcg atggtttcga
     7381 caggggaatc caccgtcaac acgccgtcca cacccgccat tgcggcttcc tgagcaaact
     7441 cccgataacc catcttatgt acagggttca aatatcccat caaaacaacc ggcgtttgcg
     7501 tgtcggtttc acggaatttt ctgacgacat ccaagacatc gcgcagcgaa atcccgtttg
     7561 ccaacgcccg ctccgccgca cgctgaataa ccggtccatc cgccatcgga tcggaaaacg
     7621 gcacgcccaa ctccaaaata tccgcaccgt ttgcaaccat gccgtgcatc aatgccaaag
     7681 ttgtccgaat atcggggtcg cccaccgtaa tatagggaat caatgccttt ccgccatcca
     7741 aagcggcaaa agcctgccgg attctgctca tttgtctctt ccatcctatc ggcaaatatg
     7801 ccataaaaca caatcagtaa atttttccca gtataacatt tgcggtacgg ataggcgaat
     7861 acggcaaaca gaaaccacgc aaatcagagc cgccgtttga acgaggcgga aataaacggc
     7921 aagcatctcg gattatagtg gattaacaaa aatcaggaca aggcggcgaa gccgcagaca
     7981 gtacaaatag tacggagccg attcacttgg tgctccagca ccttagagaa tcgttctctt
     8041 tgagctaagg cgaggcaacg ccgtactggt ttttgttaat ccactataac aaaattcaga
     8101 atcaagacag ggcaatacca tactggtttt gcaccaaccc gctataaaat tgcataaaaa
     8161 aaatagacgc tgtaaaacac agcgtctatt ttttatctgg cggagtaaga gggattcgaa
     8221 ccctcgatgc aggttgaccc cacatgcttc cttagcaggg aagtgccttc agcctctcag
     8281 ccattactcc taaggaggct gtaactataa tcaacttttc cgataccgtc aaggaaaaac
     8341 agaaagcaaa tattaatctt ttgaaataaa ataagattta ccctgattat acccaatcag
     8401 gcacttcttt tcttcctgaa tggaaatatt cctgactgcc acatcctcaa gcaaaatgct
     8461 tttgtgcatc ctgtttgatt tcctgaaacc acgcgtcttc aggaaaacgt gcaagcgcga
     8521 catccaatgt tgacagcagg ttttgcgaca gggctttggt caggtattcc tcctcgttgg
     8581 cttctgccgc tttgtcgggc tgttgtgcca aaccgtcggc acgggctttg tgtccgtgtg
     8641 ccaaatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat
     8701 agtacgacaa ggcgaggcaa cgccgtactg gtttaaattt aatccactat aattgagcgc
     8761 ggtaattttt tgcgcccaaa gctgcggtat gtcgggattg tcttcaatca tgacggaaag
     8821 gatggatacg gcggtcgcca gcctgccgcc gttcattcta aggtcgaggg aacggttggg
     8881 cggcagggtg ctgagtgttt ctgccattct gaaccaaaat tcgccgctgg cttcgtcggg
     8941 tgcgttcaat ttaagcattt catagtgaac ctcctgatca tcgttttgca cgagacccca
     9001 tacttcggca aggattttct gttcttcacg gcttaagtgc tgccactttt caatatgttg
     9061 ctgcatatct tttccctttg aacctgtttt atacggtctg aaatacggcg cggaccctgt
     9121 ccaaatcttc ctgcgtatcc acaccggcgg cgggggcttc tttggcggtt tcgacggcaa
     9181 tcgagtagcc gtgccacagg acgcgcagtt gctccagcga ttcaatggtt tccagcgacg
     9241 aaacgctcat ttcggtgtag cgttgcagga agccgacgcg gtaggcgtag atgccgatat
     9301 ggcgcaggac ggcggtttca aacggcattt cgcgttttcc tgcacgcatc gcatcgcgcg
     9361 gatagggaat cggggcgcgg ctgaagtaga tggcgttgcg gtttttgtcg aggacgactt
     9421 tgacggcatt gggattcatc aattcgtcga aatcgtgcaa ttcgtgtgcc gcagttgcca
     9481 tctgtacgtt gttttccacc aagacttcgg cagtgcggtc gatgagttcg ggggcaatca
     9541 gcggctcgtc gccctgtacg ttgacaacca ccagatgcgg cggcagcttc agcgcggcag
     9601 aggcttcggc aaggcgcgtc gtgccgcttt cgtgccggtt tgaagtcatg acgacttcga
     9661 cgccgtgcgc ctgacaggcc gtctgaatat cgggatggtc ggtggcaacg acgacgcgca
     9721 cggctttact ttttgccgcc tgttcggcaa cgcgcacgac catcggttta ccgtggatat
     9781 ccgccaaggc ttttccgggc aggcgcgatg aatccagccg cgccggaatc aatacgacga
     9841 attcagtcat gctttgagtt cctcttcgct caacgctcgc gcttcgtttt ccagcatata
     9901 gggaatgccg tctttaatcg gataggcaag cttcgcctga cggctccaca attcctgttt
     9961 gtcctgatga tattccagcc tgcctttggt aacggggcag acgaggatgt ctaagaattt
    10021 tttttccata gggttgcttt cgggtttgat ggttcaatca atcggatgcc ctcttcaata
    10081 tttcagacgg ctatcattta tttctgatgc cttttcccaa cctgtccggc ttgtttacag
    10141 cccgcatcgg gatttttacc gccgcagatg atgcgccgca actcttccgt aaacgcgggc
    10201 agatgatgtc gcgcgatttt ccctgacaat gcagtccaag atactgctat ttcactcatc
    10261 gatttcttga ttaagcgcgg atttttggca acgacggact gaccgacagg cgaaccgtaa
    10321 aaggcaatca tgccgtcaat ttcttcttgc gtgtatatct cacgggcatt cttcaataaa
    10381 gtattgcgga cagcctgttt cacttcgggc gtaatcaaat ctttcaaaac attctcacga
    10441 tagcggttaa aggcttttgc cgcctgatct tttttcgctt ccggcatttc ggcaagggct
    10501 ttgtccgcat acggtttaaa tccggcatta aagccctcaa tcatattttt ttctatatcc
    10561 cggtcaaaat tctgcgtatc cagccaacgc gccaacgacg cgtcgctggg cggggcggca
    10621 aaagcgttgg tgcacaatgc cagcgtggcg aagggcaata acaaggtttt cagtttaata
    10681 ttcaatactt taacgaatga ttaatgcaga taaaaaatac gaccgatttt ctcattcctg
    10741 tccaagtgga aatccggaga tttgggattt ctgaagcctc ctaggcattc ctgaaaattc
    10801 gagacttaga ttcccgcctg cgcgggaatg acggcgcgag gcgttcttat tcaaatccgt
    10861 aatcactccg cccaaaccgt gctttcagac ggccttcggt acatcttcca accgctccaa
    10921 cacaaacgcc gccaaatcag gttcgattat cgcacaaacg ggcaacaccc aaacattatc
    10981 ggtgcaaatg ccgtctgaaa atttgaccgc atctttctcc gtaataatga ccgcgtccgc
    11041 atcgggcaaa tctgccgccg aaatgtcggc gtggtcaggc agcgcgacgg ttcgcttcac
    11101 ggtaatgccc atattccgca acgaatcgaa aaaacgcgcc ggcttggcaa tacctgccac
    11161 ggcgacgacg gtttgatttt tcagacggcc tgtgtccagt atttcggacg gattgttcaa
    11221 acggtaaatc cgtcccgctt cgatacggct gtgaaacata ttttcagacg gcctaaacag
    11281 cgcatccgcc ttgccgccgc tgacgacgac cgcatccacc gaatccagcc gcaacaaagg
    11341 ttcgcgcaaa ctgccgttgg gcagtaaatc caaatccgtg cgccccgtat ccgccgacgg
    11401 aaacaccgcg atttccacat ctcgccgcag ggcgtaatgc tgcaaaccgt cgtccgccac
    11461 aatcagtccg atgtcgggat gcgccgccag caacgccctg cctgcctctg cacggctgct
    11521 gcccaccgcc gtcggcgcac cggttttgcg gaacagcagc aaaggctcat cgcccgcatc
    11581 ttccgctcgg ctctcagcat tcaatacatg aaccgccttg ctcttgcgcc cgtagccccg
    11641 gctgatgatg ccgaccttga cgcccttttc ctgcaaaccc gacaccagcg cggcaacaat
    11701 cggcgttttc cccgtcccac ccgcgtgaat attgccgacc acgaccacag gcacgggcag
    11761 cttttcgctt tgccgttttc ccgataaaaa atccgtccgc cgttttgccg caattttggc
    11821 aaacagcccg gagagcggct taagcagaaa agacaaaacc ggataaggtt tttgccaatg
    11881 ccgttcgata acggtatgaa atttgaaaca attcggcata ttcgatacaa atcctcgaag
    11941 caaaatcagc ccggggcatt aatcatgcgt acccgataca gacccaccta taaacaacgc
    12001 gaagaccaaa ccgataagcg aaccaatcag gtaaataagc aactgcgctt tacagaaatt
    12061 ggcgcggttc gggttgcctc tgccgaacac ccaaacaaaa attgaccaaa ggaatcatga
    12121 aaacaatcat cgtcaacacc cactgccctg tcgtcatgac gggaggcggt ttgttttgaa
    12181 gtctcgggca tatcgccaat atgataacgg acaggtttca tctctttcat tttccagtat
    12241 cttt
//
LOCUS       DAXHKX010000037        12232 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHKX010000037 DAXHKX010000000
VERSION     DAXHKX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.037"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            245..982
                     /locus_tag="ACFMWZ_000864"
     CDS             245..982
                     /locus_tag="ACFMWZ_000864"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC5934849.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWLSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            984..1670
                     /locus_tag="ACFMWZ_000865"
     CDS             984..1670
                     /locus_tag="ACFMWZ_000865"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC5934850.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPIA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1829..2692
                     /locus_tag="ACFMWZ_000866"
     CDS             1829..2692
                     /locus_tag="ACFMWZ_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC5934851.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2754..3323)
                     /locus_tag="ACFMWZ_000867"
     CDS             complement(2754..3323)
                     /locus_tag="ACFMWZ_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC5934852.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFDDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3481..4341
                     /locus_tag="ACFMWZ_000868"
     CDS             3481..4341
                     /locus_tag="ACFMWZ_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC5934853.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTLETFDHI
                     LKQLGIDYRP"
     gene            4476..4814
                     /locus_tag="ACFMWZ_000869"
     CDS             4476..4814
                     /locus_tag="ACFMWZ_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934854.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5182..5535
                     /locus_tag="ACFMWZ_000870"
     CDS             5182..5535
                     /locus_tag="ACFMWZ_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC5934855.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5525..6391
                     /gene="atpB"
                     /locus_tag="ACFMWZ_000871"
     CDS             5525..6391
                     /gene="atpB"
                     /locus_tag="ACFMWZ_000871"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC5934856.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6448..6684
                     /gene="atpE"
                     /locus_tag="ACFMWZ_000872"
     CDS             6448..6684
                     /gene="atpE"
                     /locus_tag="ACFMWZ_000872"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC5934857.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6755..7225
                     /locus_tag="ACFMWZ_000873"
     CDS             6755..7225
                     /locus_tag="ACFMWZ_000873"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC5934858.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7230..7763
                     /locus_tag="ACFMWZ_000874"
     CDS             7230..7763
                     /locus_tag="ACFMWZ_000874"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC5934859.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7774..9321
                     /gene="atpA"
                     /locus_tag="ACFMWZ_000875"
     CDS             7774..9321
                     /gene="atpA"
                     /locus_tag="ACFMWZ_000875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC5934860.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVVSVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9346..10221
                     /gene="atpG"
                     /locus_tag="ACFMWZ_000876"
     CDS             9346..10221
                     /gene="atpG"
                     /locus_tag="ACFMWZ_000876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC5934861.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10259..11656
                     /gene="atpD"
                     /locus_tag="ACFMWZ_000877"
     CDS             10259..11656
                     /gene="atpD"
                     /locus_tag="ACFMWZ_000877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC5934862.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11667..12089
                     /locus_tag="ACFMWZ_000878"
     CDS             11667..12089
                     /locus_tag="ACFMWZ_000878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC5934863.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         2997 a         3132 c         3161 g         2942 t
ORIGIN      
        1 tttcaattat ttgccgtttt agtgcgaacc gctgcctttg cctgtttcag acggcattgt
       61 ccgaaatggt tgcccgcttc ctgccttatt gacaaaaaaa cgctttcccg ataatatcct
      121 acgaaaatta acctgccgat ttgacacagc ttgcgggcat aacagctaaa gcgttccgac
      181 aatttcagct ttatcttccg cgcccgttgt gtccgacatc gggctttgtt gtatgggaaa
      241 gacaatgatt attttggaca aggtttccaa gcattaccaa acgcgcgaca agacccgttt
      301 tgccgccgtc gagccgacca gcctcgaaat ccgcgacggc gaaatcttcg ggctgatggg
      361 ttattcgggt gcaggcaaat ccaccctgtt gcgcctgatt aacctgttgg aacgccccga
      421 cagcggcaag gtcaacgtct gcggacaaga gctaaccgcg ctcgatgccg ccgcattgcg
      481 tcaggctcgg cagaatatcg gcatggtgtt tcagcagttc aacctgttga acaaccgcac
      541 cgtcgccggc aacgtcgcct tcccattgga aatcgccgga tggctgtctg aaaaaatcaa
      601 agcgcgcgtt gccgaatgtc tcgaaatcgt cggactgacc gaacgcgccg gccactatcc
      661 cgcccagctt tccggcgggc agaaacaacg tgtcggcatc gcccgcgcgc tcgcatccaa
      721 gccgcaagtc atcctcgccg acgaacccac ttccgccctc gaccccgcca ccacgcgcag
      781 cgtcttggaa tgtttggaag acatcaacaa acgcttcaac gtaaccatcg tcatcgtaac
      841 ccacgaaatg agcgtcatca gacgactgtg cgaccgcgcc gccctgttgg ataagggaaa
      901 agtcgtcgaa atcgtcgaag tacgcggcaa ccaaatccac gcccgatccg acatcgggcg
      961 cgaactcatc agagaggact gaaatggcag acctgacctt ccaacaagcc gtttccaaca
     1021 tcatcggtat gaaaggcgaa atcgtccgcg cattgggcga aaccttcgtg atggtcggct
     1081 tgtccaccac attcgccgtc gttttcggca cgttgctggg cgtgttgctc ttcgtaactt
     1141 ccagccgcca actgcattac aacaagccgg tgaacttcct gctcgacaac ctcgtcaacc
     1201 tcatgcgcgc cttccccttc gtcattttga tgattgcgat gatacccgcc acacgcgcca
     1261 tcgtcggcag caccatcggc ccgattgccg cctcgctggt gttgagcgtg tcgggattgt
     1321 tttattttgc ccgactggtg gaacaaaacc tgcgcgaagt ccccaaaggc gtaattgaag
     1381 ccgccgccgc gatgggtgcg ccgccggttg ccatcgtccg caaagtcctc ttaaacgaag
     1441 cgcgcgcggg catggtttcc agcattaccg tgcttgccat cgggcttttg tcatacagcg
     1501 cggcggcagg gatgataggc ggcggcggct tgggcgacct cgccatccgc tacggctact
     1561 accgctacca aaccgaagtc atcgtcttca tcgtcgccct cctcgtgctg ctggtcatcc
     1621 tgattcaaag caccggcaac gcattggcgc ggaaattgga taaacgttaa acccgaatgc
     1681 cgtctgaacg ccaaaacccc caccgctatc cgaaaaatgc tataaaatcc ccctgttcgc
     1741 ggcaaatgcc gtctgaacgc cgaatctgga cggcaggact ccctgcccgt catttttgtt
     1801 tgaaactgcc acaacatcag gagaaaatat gaaaaccttc ttcaaaaccc tttccgccgc
     1861 cgcactcgcg ctcatcctcg ccgcctgcgg cggtcaaaaa gacagcgcgc ccgccgcatc
     1921 cgcttctgcc gccgccgaca acggcgcgga gaaaaaagaa atcgtcttcg gcacgaccgt
     1981 cggcgacttc ggcgatatgg tcaaagaaca catccaaccc gagctggaga aaaaaggcta
     2041 caccgtcaaa ctggtcgagt ttaccgacta tgtgcgcccg aatctggcat tggctgaggg
     2101 cgagttggac atcaacgtct tccaacacaa accctatctt gacgacttca aaaaagaaca
     2161 caatctggac atcaccgaag tcttccaagt gccgaccgcg cctttgggac tgtacccggg
     2221 caagctgaaa tcgctggaag aagtcaaaga cggcagcacc gtatccgcgc ccaacgaccc
     2281 gtccaacttc gcccgcgtct tggtgatgct cgacgaactg ggttggatca aactcaaaga
     2341 cggcatcaat ccgctgaccg catccaaagc ggacattgcc gaaaacctga aaaacatcaa
     2401 aatcgtcgag cttgaagccg cgcaactgcc gcgtagccgc gccgacgtgg attttgccgt
     2461 cgtcaacggc aactacgcca taagcagcgg catgaagctg accgaagccc tgttccaaga
     2521 accgagcttt gcctatgtca actggtctgc cgtcaaaacc gccgacaaag acagccaatg
     2581 gcttaaagac gtaaccgagg cctataactc cgacgcgttc aaagcctacg cgcacaaacg
     2641 cttcgagggc tacaaatccc ctgccgcatg gaatgaaggc gcagccaaat aaggcagtcg
     2701 tataaaatga tgccgtctga accgtatccg tgttcagacg gcatttttgt cctttaatcc
     2761 gccattccct gccattccgc cgaatccggc gtatcgattc cgaacagcga caaagcgtgt
     2821 gcaacactgt gcgccactat gtcgtccgcc gtctgcggtt tgcggtacat cgcaggaaca
     2881 gggggaaaca ccacgccgcc catttccgtt acccgcttca tattgtccaa atgggcaagg
     2941 ttcagcggcg tttcgcgcac catcagcacc agccgccgcc tttccttcaa aaccacatcc
     3001 gccgcacgcg tcaagaggtt gtcgtcgaag ccgtgcgcga ccgaggcaag cgtccgcatc
     3061 gaacaggggg cgaccagcat cccgtccgtt ttaaacgtac cgctggcaat gcacgccccg
     3121 atattgccga tcggatgcac gaagtccgcc aaggcatata cctcatctct cgcataagcc
     3181 gtttccgaag cgcgcgccat ctccgcacct ttcgatacca caaggtgcgt ttcgatatct
     3241 tgcgcgcgta aaagttccaa agccttcacg ccgtattgga aaccgctcgc cccactgatg
     3301 ccgattatca aacgccgtac catcatccgc ctttcccata aaaccgcctg caacggcaaa
     3361 ccggctatta tagtgaaaaa acagaaatcc gataaatacg gatacaaatt gtcggcaaca
     3421 cccaatatcc gataaaatat ccgatttaac atcctatctg aataggcacg ggagggcggt
     3481 atggcaaaag taaaaggcgg attggggcgc ggtttggatt cgctgctcgc caacggcgca
     3541 gacaacagca gcggcgaccg attgaccacg gttgcggtta aagatatccg gcccggccgc
     3601 tatcaggcgc gtgttcaaat cgatgacgaa gccttgcagg agctggcgga ttcgattaaa
     3661 gcacagggag tgatacagcc cgtcatcgtg cgcgaacacg gactgtcccg atacgaactg
     3721 attgcggggg agcggcgttg gcgtgccgca cagattgccg gcctgaccga aatccccgcc
     3781 gttatcaaaa ccatcagcga cgaaaccgcg ttggcaatgg gtttgatcga aaacctccag
     3841 cgcgaaaacc tcaaccccat cgaagaagca caaggcttga aacgccttgc cgacgagttc
     3901 ggactgaccc acgaaaccat cgcccaagcc gtcggtaaaa gccgaagcgc gatttccaac
     3961 agcctgcgcc ttttaagcct gcccgaaccc gtgcaggaaa tgctttacca acgccgcctc
     4021 gaaatggggc acgcccgcgc actgctgacc ctgcccgtcg tcgaacagct cgaattggcg
     4081 caaaaggccg tcaaaaacgg ctggtcggtg cgcgaagtcg aacgccgcag ccaagccgcc
     4141 cttcaaaaca aacgtcccga gcccaaaaag actgccgccg ccgacatcgg ccgcctgaat
     4201 gatttgctga ctgaaaaact gggtgtcaac gccgaaatca aaaccgccaa ccataaaaaa
     4261 ggcaaaatca tcctgcattt cgacacgctc gaaaccttcg accatatctt gaagcagttg
     4321 ggcatagatt accgacctta attttgcggg atataccgtc tgaaatatag agaatagctt
     4381 tccagatttt aagtgggaaa tataattcta ttgacatttt tctgcttcac gtaagaatcg
     4441 ttttcctgtt ttcattttta attttcgagg aaattatgaa cacacgcatc atcgtttcgg
     4501 ctgcgttcgt tgcgttggca ttagcaggtt gcggctcaat caataatgta accgtttccg
     4561 accagaaact tcaggaacgt gccgcgtttg ccttgggcgt cagcccaaat gccgtaaaaa
     4621 tcagcaaccg caacaatgaa ggcatacgca tcaactttac cgcaactgtg ggtaagcgcg
     4681 tgagccaatg ctatgttacc agtgtaatca gcacaatcgg cgttaccact tccgatgcaa
     4741 tttgtttggg aggcggaacg cacaaaggca aaagtcaatg caatgctttg cttaaagcgg
     4801 caggccgttg ctaatccttt attcggaaaa ggtcgtctga aaatattttc agacgacctt
     4861 ttatttattg agcaaattcc ccaaactgcc gacaatccga ccgataaact gctacaattt
     4921 cgcgttgatt ttttattatt tccacgtaaa gcaaacaaat cacgtaagaa cattaaaaat
     4981 gtaaagaaat tgcggacaaa ctccttaata tcctgtaatt atatgtaatg tgtgttagaa
     5041 tgacacctgt cgccgttatt caggcggcga gcgcggcgga ttgggatacg ttgtttcaca
     5101 aagattaact gtcgcttgac gtaataaggc ttctaaatta tattagccgc gccccaaatc
     5161 cgaggcggaa acattccctt tatgaagcag attatcatcc tacaatccgc cgtattgtcc
     5221 atatgcgccg cagttgcctt tgctgtatgg ggttttgccg gattcctttc ggcggtcggt
     5281 ggcggtttgt cctacctgct tcccacgttt gttgcagttt tacttttaaa acttttcagg
     5341 ggaaacccct tcctgcaaag caggatgttc gtcttcggag agattttaaa agtagtgctg
     5401 tcgctgttgt ccatgctcgc cgtatttgct gtatggcatc aatcgttggt gttcgccccg
     5461 tttctgatgg ggctgctcgg tgtcagccat ttggtttttt tagtattgtt gagagtgaaa
     5521 gattatggca ggtgaaacca ttaccgctgc cgactacatc aagcaccact tgcagagctt
     5581 gaccagtttg tcggatgtta ctcagggtca gggactgaaa aacattgctg atttttcgtt
     5641 tattaacctt gatgccgtct tttttgccgt cctgttgggc gtaatcggca gcttcctgtt
     5701 gtggcgcggt gcgaaaaaag caacggcagg cgttcccgga cgtttccagg ctgccgtgga
     5761 aatcttgttt gagtttgtgg acgatatgtg taagagcatc attcacaacg aaaagtctag
     5821 aaaagccgtc gcgccgttgg gtctgacgct gtttgtctgg atttttctga tgaacgcgat
     5881 ggatatgctg ccggtcgatt tgctgccgat ggtatggcag ggcattaccg gcaaccatca
     5941 cgccctgctg cgcgtcgtac cgactgccga tttgaacacc actttggcac tcgctgtcgg
     6001 cgtgttgctg atttgtatct attacaacat caaaatcaaa ggattgggtg gctggtttca
     6061 cgagttgttc agcgcgccgt tcggtgcaaa actcgcacct gcgaactttc tgttgaattt
     6121 ggtcgagttt ctttctaaaa ccgtatccca cggtatgcgg ttgttcggca atatgtatgc
     6181 gggcgaattg gtattcttgc tgatagcctt gcttggcggt gcttgggcgg cttccggcag
     6241 cgtcgaagta atggatccga ttctgtttgt attccacatt attgccggtt tggcatgggc
     6301 gattttccat attttggtga ttaccctgca ggcgtttatt ttcatggcgt tggcgttcgt
     6361 ctatatcgga caggcacatg atgcgcactg attttcctaa gtagtttgtt tttgtagttt
     6421 aacctttgtt tctttaagga gtttaaaatg ggtttgattg ctatcgcatg tggtttgatc
     6481 gttgcattgg gtgcattggg tgcctctatc ggtatcgcaa tggtcggttc taaatatttg
     6541 gagtcttctg cccgtcagcc cgaactgatc ggtccgctgc aaaccaaact gttcctgatt
     6601 gccggtttga ttgatgccgc attcttgatt ggtgtcgcca ttgcactgct gttcgccttc
     6661 gttaacccgt ttgcaggtgc ataatcagga cggattcgtc cgttccgtac aggcgcagcc
     6721 tgtttgatta accccagtac gaaggttaag taacgtgaat atcaatgcaa cattattcgc
     6781 tcaaatcatc gtctttttcg gtttggtatg gtttaccatg aaattcgtgt ggccgccgat
     6841 tgcaaaagct ttggatgagc gtgccgcaaa agtcgccgaa ggcttggctg ccgccgagcg
     6901 tggtaaaagc gatttcgagc aggctgaaaa aaaggttgca gaactcttgg cagaagggcg
     6961 taatcaggtt tccgaaatgg ttgccaacgc cgaaaaacgt gccgccaaaa ttgtcgaaga
     7021 agccaaagaa caggcttctt ccgaggcggc gcgcattgca gctcaggcaa aggccgatgt
     7081 ggagcaggaa ttgttccgcg cacgcgaatc cctgcgcgag caggttgccg tgttggctgt
     7141 caaaggtgcc gaatctattt tgcgcagcga agtcgatgct tccaaacacg caaaactgct
     7201 cgataccctg aaacaggagc tgtaatctta tggcagagtt cgcaacgatt gccagacctt
     7261 atgcaaaggc attgttcggt ctggctcagg aaaaaaacca aattgagtct tggttgggcg
     7321 gactggaaaa acttgcggcg gttgttcagg aagggaaggt ggcttcattg attgaccgtc
     7381 ctgaaacgaa tgcttcagaa aaagcagata tcctcatcga tttggtcggt ttgaaagaca
     7441 aggagttgaa aaactttgtt atcgtcttgg ccgggcagaa acgtttgtcg atattgccgg
     7501 aagtatatgc tcaatatcaa gacttgacct tatcattcaa ccatatcaaa tctgccgtca
     7561 tttacagtgc ctatccgttg accgacaaac aggtcggcga gttggcgcaa atgctgaata
     7621 agcgtttcga cagcgagctg aaaatctctg tcgaaatcga accggagctg attggcggca
     7681 taaaagttga agtgggtgat caggttttgg atttgtctgt acaaggcaaa ttgagtgcct
     7741 tgtacacgac tatgacgaat taggagagtt ttcatgcagc ttaatcctgc tgaaattagc
     7801 gatttgatta aagccaagat cgaaaatctg tctgtaaatg ccgaagtgcg tactcgtggt
     7861 accgttgttt ctgttacaga cggcattgtg cgcattcatg gcttgtcaga cgcgatgcaa
     7921 ggtgagatgc tcgaattccc cggtaacact ttcggtctgg ccatgaactt ggagcgcgac
     7981 tccgtcggtg ccgtagtgtt gggtgagtac gaacacatta aagaaggcga tacagttact
     8041 tgtaccggtc gtatcttgga agtgccggtc ggacgcgaac tggtcggacg cgtcgttgat
     8101 gcattgggtc ggcctatcga cggcaaaggc ccgattaata caactttgac cgcccctatc
     8161 gaaaaaatcg caccgggcgt gattgcgcgc aaatcggttg accagccgat gcaaaccggt
     8221 ctgaaggcga ttgactctat ggttcctgtc ggtcgcggtc agcgtgagtt gattattggc
     8281 gaccgtcaga caggtaaaac ggccgtagca ttggatgcca tcgtcaacca aaaaggtacg
     8341 ggtgttatct gtatctatgt cgctatcggt caaaaagcat cttctattgc caacgtggtc
     8401 cgcaaattgg aagagcatgg cgcgatggag cataccatcg tggttgctgc aactgcatct
     8461 gaagcggcgg cattgcaata tatcgcacct tactccggtt gtacgatggg tgaattcttc
     8521 cgcgatcgtg gcgaagatgc cttgattgtt tatgacgatt tgtccaaaca ggctgtggct
     8581 taccgtcaaa tttccctgct tttgcgccgt ccgcccggcc gcgaagccta tcccggcgat
     8641 gtgttctacc tgcactcccg tctgttggaa cgtgcggcac gtgtcaatga acacgaagtg
     8701 gagaaattga ccaacggcga agtaaaaggc aaaaccggtt ctctgaccgc gttgccgatt
     8761 atcgaaaccc aagcgggcga cgtatctgct ttcgttccga ctaacgtcat ttcgattacc
     8821 gacggtcaga ttttcttgga aaccgacctc ttcaacgccg gtatccgtcc tgcaatcaat
     8881 gccggtattt ccgtatcccg cgtaggcggt gctgcacaaa ccaaagtgat taaaaagctg
     8941 ggtggcggta tccgtttggc gttggcgcaa tatcgtgaat tggcggcgtt ctcgcaattt
     9001 gcatccgatt tggatgaagc tacgcgcaaa caattggagc atggcgaagt cgtaaccgaa
     9061 ctgatgaaac agaaacagtt cagtacgttg aatacggctg aaatggcttt gaccctttgg
     9121 gcaatcaaca acggttcgta ttctgatgtt ccggttgcca aagctctggc ttttgaatct
     9181 gaatttttga gctttgtccg tacccaacat ccggaagttt tggaagccgt caatgcttca
     9241 ggtgcaatgt ccgacgagag cgagaaaacg cttgaagcag ccatgaaatc cttcaaatct
     9301 tcttacgcct atcaggcata aggcttgaag tgaaaggagt ctgaaatggc agtaggaaaa
     9361 gagattctca ccaaaatccg cagtgttcag aatacccaaa agatcactaa agcgatgcag
     9421 atggtgtcaa cctctaaaat gcggaagact caggaacgga tgcgtttggc gcgtccgtat
     9481 gccgaaaaag tgcgtatggt gatgagccat cttgcgcaaa ccaataccga tcatggtatt
     9541 ccgttactgg aatctcatcg ggaaatcaga cgtgtcggtt ttattttgat tacgtctgat
     9601 aagggtttgt gtggcggttt gaacgccaac gtgctgaaaa agtttttggc acaagttcaa
     9661 gagtatcgga atcaaggtat tgaagaggaa atcgtatgcc ttggcagtaa aggtctgatg
     9721 gcgtgtcaga gcattggtct gaatgtggtt gccagtgccg taaatttggg cgatacccca
     9781 aaaatggaaa tgctgctcgg acctttgaca gaactcttcc aacggtatga gaaacatgaa
     9841 attgacagaa tccatctggt gtattcgggt tttgtcaata ccatgcgtca agaaccgaga
     9901 atggaagtat tgctgcctat cggtgagaac gtgattggcg attcagctcc caaatcaccg
     9961 ttcagctggg aataccgcta cgaaccgact gcacttgcag tgttggaata tttggttcgc
    10021 cgctatttag agtctgtggt ttatcaggcg ttgagcgaca atatggcatc cgaacaggca
    10081 gcccgcatgg ttgccatgaa agctgcaaca gacaatgcag gcaatgccat caaagagttg
    10141 cgtttggtat ataacaaatc gcgtcaagct gcgattacca cggaattgtc agaaattgta
    10201 gcaggtgcgg cggctgtctg atgccgtctg aatcctgtac ggaaattagg atacgataat
    10261 gagccaaggc aaaatcgtac aaattatcgg tgcggttgtt gacgtggaat ttccacgcga
    10321 catgattccg cgcgtttacg acgctttgaa attagacgaa aacggtctga ctttggaagt
    10381 ccaacagctt ttgggcgacg gcgtagtccg taccattgcg atgggcagct cggacggttt
    10441 gaaacgcggc atgactgtga gcaataccgg tgcgcccatt actgtgccgg taggtaaagg
    10501 tacgttggga cgcattgtcg atgtattggg aacgcctgtt gacgaggcag gtccgatcga
    10561 taccgacaag agtcgtgcca tccaccaagc cgctcctaag tttgacgaac tgtcttccac
    10621 aaccgaattg ctcgaaacgg gcattaaagt gattgacttg ctgtgtccgt ttgccaaagg
    10681 cggtaaagta ggtctgttcg gcggtgccgg tgtgggtaaa accgtgaaca tgatggaatt
    10741 gatcaacaac atcgccaaag cgcacagcgg cttgtccgtg ttcgcaggcg tgggtgagcg
    10801 tacccgcgaa ggtaacgact tctaccacga gatgaaagat tccaacgtat tggataaagt
    10861 ggcgatggtt tacggtcaga tgaacgaacc tccgggcaac cgtttgcgcg tcgcattgac
    10921 cggtctgacc atggcggaat acttccgtga tgaaaaagac gaaaacggta aaggccgcga
    10981 cgtattgttc ttcgtggaca atatctaccg ttacaccctg gccggtaccg aagtatccgc
    11041 attgttgggc cgtatgccgt ctgcagtggg ctaccaaccg acattggcag aagaaatggg
    11101 tcgtttgcag gagcgtatta cctctaccca aaccggttct attacttcca tccaagccgt
    11161 atatgtacct gcggacgact tgaccgaccc gtctccggca acaacgttcg cccacttgga
    11221 cgcaaccgtc gtattgagcc gcgatattgc ctctttgggt atttacccgg cagtcgatcc
    11281 gcttgattcc acttcgcgcc aactggatcc gatggtcttg ggtcaagagc actacgacgt
    11341 ggcgcgcggc gtacagtcca ccctgcagaa atacaaagaa ttgcgcgaca tcatcgccat
    11401 cttgggtatg gacgaattgt ctgatgaaga caaactgacc gtaatgcgtg cgcgtaaaat
    11461 ccaacgcttc ctgtcccaac cgttccacgt tgccgaagtg tttacaggtt ctccgggtaa
    11521 atatgtcgcc ctgcgcgata ccattgccgg cttcaaagcc atcttgaacg gcgaatacga
    11581 ccatctgccc gaacaggcat tctacatggt cggcagcatc gaagaagcgg ttgagaaagc
    11641 gaaaacctta aactgaggag gtcggcatga gcatcatgca agttgaggtg gtcagtagcg
    11701 agcaaaacat ctattcgggc gaagccagct ttgtagtggt tccgaccgtt caaggtgagc
    11761 ttggtattta cccgcgacac gagccgatta tgagtttggt gcggccgggg gctttgcgtt
    11821 tgaccgttcc gggcgaggat aaagaggttt tggttgctgt ttccggcggt gttttggaag
    11881 tacagcctga taaagtaact gtcttggcgg atgttgccgt ccgcagtgcg gagatggatc
    11941 aggcacgtgc ggaagaggcg aaaaaagccg cagaagcggg catttcccaa gctaaagacg
    12001 ataaggcttt ggcggaggcg cataaagcat tggctgctgc gattgcacag ctcaaaactt
    12061 tggactatat ccgttcgcac aagaaataaa cttgtttggt ttggaaaagc acgaccgttt
    12121 ggtcgtgctt tttattttcc ggatttaaat taaagactgt gtttggatat ggcgttggca
    12181 ggcatagcct gctttaaggt attcaatagg gcagcctgcc gcctaaccga aa
//
LOCUS       DAXHKX010000038        12125 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHKX010000038 DAXHKX010000000
VERSION     DAXHKX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.038"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..549
                     /gene="mafB"
                     /locus_tag="ACFMWZ_000879"
     CDS             <1..549
                     /gene="mafB"
                     /locus_tag="ACFMWZ_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC5934864.1"
                     /translation="NTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKA
                     AVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNY
                     IEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGT
                     IHSGKNGVHIVPANPKTIKKVQ"
     gene            552..869
                     /locus_tag="ACFMWZ_000880"
     CDS             552..869
                     /locus_tag="ACFMWZ_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934865.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            1003..1680
                     /locus_tag="ACFMWZ_000881"
     CDS             1003..1680
                     /locus_tag="ACFMWZ_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC5934866.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYR
                     ETVYIKVSDGIGKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPPRKTPSTPIYGNDSEAYAAAK
                     ELGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPEKLNRKETRNGTFDK
                     NLNRIGD"
     gene            1686..2093
                     /locus_tag="ACFMWZ_000882"
     CDS             1686..2093
                     /locus_tag="ACFMWZ_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC5934867.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASTWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLRRKV"
     gene            2115..2492
                     /locus_tag="ACFMWZ_000883"
     CDS             2115..2492
                     /locus_tag="ACFMWZ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC5934868.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            2534..2800
                     /locus_tag="ACFMWZ_000884"
                     /pseudo
     CDS             2534..2800
                     /locus_tag="ACFMWZ_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
     gene            3097..3369
                     /locus_tag="ACFMWZ_000885"
     CDS             3097..3369
                     /locus_tag="ACFMWZ_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934869.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIHEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            3536..3766
                     /locus_tag="ACFMWZ_000886"
     CDS             3536..3766
                     /locus_tag="ACFMWZ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC5934870.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            3917..4654
                     /locus_tag="ACFMWZ_000887"
     CDS             3917..4654
                     /locus_tag="ACFMWZ_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC5934871.1"
                     /translation="MVKTADGYKAIAHIRVGESVLSKDEASGKTGYKPVTARYGNPYR
                     ETVYIKVSDGIGNNQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            4651..5070
                     /locus_tag="ACFMWZ_000888"
     CDS             4651..5070
                     /locus_tag="ACFMWZ_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC5934872.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            5237..5905
                     /locus_tag="ACFMWZ_000889"
     CDS             5237..5905
                     /locus_tag="ACFMWZ_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC5934873.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTAQCGNPYR
                     ETVYIEVSDGIGNSQTLISNSIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPKRAPEYHAGTVSESNFLNAA
                     EKWLGENYKSYPNGRYVSQDGMRQVRYGYHETNSSTHHGHFESYDKPNGRVIENSAVT
                     IIRD"
     gene            5909..6181
                     /locus_tag="ACFMWZ_000890"
     CDS             5909..6181
                     /locus_tag="ACFMWZ_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934874.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            6302..6847
                     /locus_tag="ACFMWZ_000891"
     CDS             6302..6847
                     /locus_tag="ACFMWZ_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC5934875.1"
                     /translation="MITVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAEWVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFTW"
     gene            7252..7653
                     /locus_tag="ACFMWZ_000892"
     CDS             7252..7653
                     /locus_tag="ACFMWZ_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934876.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DDIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            7835..8224
                     /locus_tag="ACFMWZ_000893"
     CDS             7835..8224
                     /locus_tag="ACFMWZ_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934877.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDIPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(8265..9722)
                     /locus_tag="ACFMWZ_000894"
     CDS             complement(8265..9722)
                     /locus_tag="ACFMWZ_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC5934878.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(9737..10567)
                     /locus_tag="ACFMWZ_000895"
     CDS             complement(9737..10567)
                     /locus_tag="ACFMWZ_000895"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC5934879.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(10645..11034)
                     /locus_tag="ACFMWZ_000896"
     CDS             complement(10645..11034)
                     /locus_tag="ACFMWZ_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC5934880.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAIAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(11170..11694)
                     /gene="nspA"
                     /locus_tag="ACFMWZ_000897"
     CDS             complement(11170..11694)
                     /gene="nspA"
                     /locus_tag="ACFMWZ_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC5934881.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDASYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            11899..>12125
                     /locus_tag="ACFMWZ_000898"
     CDS             11899..>12125
                     /locus_tag="ACFMWZ_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934882.1"
                     /translation="MKLANGLEKTAVSVGLLQVQAYGFVCVCALSVGHFRRRLCRLKR
                     PAGDAAAFSIDKHIFRTRSGRVFPGGRRFVCA"
BASE COUNT         3595 a         2366 c         3042 g         3122 t
ORIGIN      
        1 aatacgcgcg aagccgttga ccggtggata caggaaaacc ccaatgccgc cgaaacggtc
       61 gaagccgtct tcaacgttgc cgctgccgcc aaagtcgcga agctggcaaa ggcggcgaaa
      121 ccggggaaag ctgcggttag tggggatttc tcagactcct acaagcataa cactgcttca
      181 agattatctc agtctgtaga tggagaaatg tttcaaaccc gcaatgttga ttttaaagca
      241 aaatctattg ggactaaaat tcatgatgga gctcaaggga aacatatttc aggacataga
      301 aactacattg aaggtaagag tactttaaat caaaacatta atcctcaaga attgttgaac
      361 ggaatacatt caggtgctta tccagttatt tctaaaggag caagaggaaa tcctgttgtt
      421 gattttgggt atcctatagg cagcgatggg aaatcaggat taagtaccaa ttttggtacg
      481 attcattcag gtaaaaatgg agttcacatt gttccggcta accctaaaac cattaaaaag
      541 gtgcaatagt tatgaatata ttaccaagct ggctgcgagt cggtatgaat atagcaatgc
      601 tgggcatgat acactcagat atcaggttaa ttaccgtaga ttacgaggaa ggaagaaggt
      661 ttttaaaaat caaaaattat ttatcaagag aagccatcac agaagaccat gaagatatgg
      721 aatatttgat tacagagtta tggtctatgt gtggagaata ttttgatgaa gctgactttg
      781 aatgtattta ttctaatcat tcttctatgg agttaaacca aataaatggt gcagtattca
      841 ggagaaagga attaatttcg caagcgtagg ttaaaaaacc aacaatcaca atgtcttctg
      901 aaaccgtgtt taattttcag acggcatttc cttcatttga aataggatat tgagaactga
      961 gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac
     1021 ggctacaaag ccattgccca tattcaagcc ggagaccgcg tcctttccaa ggacgaggca
     1081 agcggagaaa cgggatacaa acccgttacc gcccgatacg gtaatccgta tcgggaaacc
     1141 gtttacatta aagtttcaga cggcatcggt aaaatacaga ccctcgtttc caataaaatc
     1201 cacccgtttt acagtcaagg aaaatggata caggcaggtc gtctgaaaaa aggcgacacc
     1261 ctgctttccg aaagcggcgc aaaacagacg gttcaaaaca ttaccttcaa acagcagccg
     1321 ctcaaagcct acaatctgac cgttgccgac tggcatactt actttgtcaa aggcgataag
     1381 gcggaaacgg aaggggtttg ggttcataat gattgtccgc caagaaaaac accatctacc
     1441 cccatatatg ggaatgattc agaagcatat gcagcagcaa aggaattggg atatagaaaa
     1501 ataaaagaaa gaacgagaaa tgatgctgct atttttaaaa aaggaaagag ttatatatca
     1561 cgcgatgtag attcacataa tggaggtgct tggaaagagg catcttcacc cgaaaaactg
     1621 aacagaaaag aaacaagaaa cggtacattt gataaaaacc taaatcgtat tggagattaa
     1681 ttattatgtg cgagttcaag gattttagaa gaaacatccc ttgttttaag gagtatgacg
     1741 aaaattcatt tattggcaaa tggtatgatg acggggtgtg ggatgatgaa gaatattgga
     1801 aattggagaa tgatttaatt gaggttagaa aaaaatatcc ttatccgatg gatataccaa
     1861 gggacatcgt gattggaatc ggtaccatta ttgatttttt aatggttcaa aattggaaac
     1921 tttttgaaat taaagcttct acttggttgc ctaaaagtgt caaaattaat gagcgttatg
     1981 aaaggttcag ggtaatgctc cgttatattt ttactgatct agatgtagag gactggaaat
     2041 ttttttactt tccaatacaa catagtaaag gtagattgag acgaaaagtt taattatatt
     2101 ggagatacat tattatgtgt gagttcaagg atattataag aaacgttcct tattttgagg
     2161 ggtatgacga aaattcattt attggcaaat ggtatgatga cggggtgtgg gatgatgaag
     2221 aatattggaa gttggagaat gatttaatcg aggttagaaa aaaatatcct tatccgatgg
     2281 acataccaag atatgttgtc attggaatcg gtaccattat tgatttctta atggttccaa
     2341 attggaaact ttttgaaatt aaagcttccc cttggttgcc tgatagtgtg ggaattcatg
     2401 aacgttatga aagattcaca acgatgctcc gttatatttt taccgagaaa gacatagtca
     2461 acgtgcgatt tgattattac aacaaaaaat agcaaaaccc ggcaatcaaa atgccgtctg
     2521 aagcgatatc cggctttcag acggcatcgg caacagccaa atcctgattt ccaaccgcat
     2581 ccatccgttt tattcggacg gcaaatggat taaagcggaa gatttgaaag cgggaagcag
     2641 gctgttatcc gaaagcggca aaacccaaac cgtccgcaac atcgttgtca aaccaaaacc
     2701 gctcaaagcc tacaatctga ccgttgccga ctggcatacc tactttgtca aaggcgataa
     2761 ggcggaaacg gaaggggttt gggttcataa tgattgtccg tatggaaatt tatcggataa
     2821 taaatctgtt ggcgaaggaa aaaaatttac accagctcaa aagaaagcaa tcatccaaga
     2881 aaacatgaat agaaacggag gggtagtgaa gtctgaccaa tctggagaag ttttggttcg
     2941 tcctaaaaag agccaaaaag gtatcacccc cccatcaaat gaatggcaga ttgaccatgt
     3001 gcaagcaaaa agtaaaggag gttcaaatag ttataaaaat gcacaggttt tatccagaag
     3061 agaaaatatt aagaaatcaa ataaataggt atcaatatgc aatggaaatt tgaagatcct
     3121 caaaatactg ctgttattac caccagaaga attttgtcta aaaaaaacgg aatcttagaa
     3181 gtatggcatg atgctgatga tggaatgtgg caattcttag atggcaatga aattcatgaa
     3241 ggagatgctg tgatagttag tttaaaagaa attgtcacta ttgattcctc agtcaatctc
     3301 ttaagtaatt tacctcttgg tggttacgct tggagaaata ctatatcgga aaaatggaat
     3361 tttgattagc aagcgtaggt taggttaaaa aaaccaacaa tcaaaggtcg tctgaaaccg
     3421 tgtttagttt ttcaaacgac ctttccttca attaacatag aatgttgggt ctcgacccaa
     3481 cctacacgct tactaatctc aaatataacc ttatgaaata atgagtattt aaaagatgga
     3541 aacattagac gaacgaataa aaaatttagg aaaatctctt gaagatagaa ttgacgctaa
     3601 tctaattgat gccgcactag agtacattac tttttcagaa cgtttgctag cttttgaaac
     3661 gctatgtgat tatatagaag atttcaatat ccagcttacg gaaaaagaat cccaagaaat
     3721 ctcttttatt aataaggaat ttggaattga aagtacatca gattaataaa tattaggtcg
     3781 gtttaaaaac ccaacaatca aaaagtcgtc tgaaactgtg tttaatttac agacgaccta
     3841 tttccttcat ttgaaacagg atattgggaa ttaagttttt caaaaatcct acacctgctc
     3901 cttccacggc agcaccttgg tcaaaacggc agacggctac aaagctattg cccatatccg
     3961 agtcggcgag agcgtccttt ccaaggacga ggcaagcggg aaaacaggat acaaacccgt
     4021 taccgcccga tacggtaatc cgtatcggga aaccgtttac attaaagttt cagacggcat
     4081 cggcaacaac caaaccctga tttccaaccg catccatccg ttttattcgg atgggaaatg
     4141 gattaaagcg gaagatttga aagcgggaag ccggctgtta tccgaaagcg gcaaaaccca
     4201 aaccgtccgc aacatcgttg tcaaaccaaa accgctcaaa gcctacaatc tgaccgttgc
     4261 cgattggcat acctactttg tcaaaggcga taaggcggaa acggaagggg tttgggttca
     4321 taatgattgt ccgtatggcg gttcaaataa tttagagaaa gcaaaacttc gcgcagaacg
     4381 gcttagtaaa aacgatcggg caggaaaaga ctttactaaa gcaggaaaag aagccgttat
     4441 tgatttgaat aggattcaaa ataatggtca agttaagtgc gctaattgcg gtattgaaac
     4501 aattccagcc aaacaatcta taaaaaatat ttctcctact tcaaatgaaa gacaagtaga
     4561 tcatgtcatc cctaaatcta agggtggtca aggtacacct aaaaacgggc aggtattatg
     4621 cagaggctgc aacattaaga agagtaacaa atgaaatcga aacttactgt cgtctattac
     4681 gatttggaaa gcaatattgc agaagaaata ctgtcaggaa acataatgcc tgatggaaat
     4741 tttcttattc aagagattcc tcttttcgca ccgaatttgg ctttaaacga cattgttgcc
     4801 atagaacgtg aggataagat gctgtttttc gaccacttga taaaagcttc aggaaatacc
     4861 acgataaaca tcgttgtttt ggatcatttc ccaaaggatt tattggcagc catagaagaa
     4921 cacagtggta aaatcagaaa aaatggagag aattatttat cggttaattt tccgcccaaa
     4981 aaatataatt ctgatttaaa aggaatttta aatagatatg aggaagcaaa tatcctcagc
     5041 tacagggaag cttgtttggg cttctcttaa tcgatctata agtaagaatg gattacgtta
     5101 gattgtaaac ctaacgatta aaaggtcgtc tgaaaccgtg tttaattttc agacgaccta
     5161 tttccttcat ttgaaacagg atattgggaa ttaagttttt caaaaatcct acacctgctc
     5221 cttccacggc agcaccttgg tcaaaacggc agacggctac aaagccatcg cccatattca
     5281 agccggagac cgcgtccttt ccaaggacga ggcaagcggg gaaacaggat acaaacccgt
     5341 taccgcccaa tgcggcaatc cgtatcggga aaccgtttac attgaagttt cagacggcat
     5401 cggtaacagc caaaccctga tttccaacag catccatccg ttttacagtc aaggaaaatg
     5461 gatacaggca ggtcgtctga aaaaaggcga caccctgctt tccgaaagcg gtgcaaaaca
     5521 gacagttcaa aacattaccc tcaaacagca gccgctcaaa gcctacaatc tgaccgttgc
     5581 cgattggcat acttacttcg tcaagggtaa tcaggcggaa acggaagggg tttgggttca
     5641 taattcttgt ccgcccaaaa gagctcccga ataccatgca ggaactgtat ctgaatctaa
     5701 ttttttaaat gcagctgaga aatggctagg ggaaaactat aagtcatatc caaatggtcg
     5761 atatgtatca caagatggaa tgcggcaggt acgatatggt tatcatgaaa caaattcatc
     5821 tacgcatcat ggtcattttg aatcatatga caaacctaat ggacgtgtaa tagaaaatag
     5881 tgcggtaaca attattcggg attgaagaat gatgagtgta aaagaattat tcaaagtcat
     5941 cttagatgaa aataaagatt ttcctattag aacgattcat cgtaccccga tggggatact
     6001 tcccaaacct gttgctttaa gtattgttca atatgaaaat gacccgggat tttatttgtt
     6061 ttatttggat gagaccggtc aggaacaaac ggatacttat catgacacat tagattcggc
     6121 atttgaacag gctgaatttg aatttggaat cagcaaagag gaatggatgc aaagtcctta
     6181 gacggcaaaa tcaagatgcc gctttatttt tcagacggca tttccgtcaa taaaaaagtc
     6241 ggtattggag cccaaaacag cctacgtctt gccgggatag ggatgaaatc tttaagaaaa
     6301 aatgattaca gtcgaacatt tagagcatta tttgggtgaa atcgaaagcg gtataaaatg
     6361 cttggatcga cgttatcatc tcagcgtttc cgtcttccca tcacaaccat ataaaggggt
     6421 aactactttt tctactttag gcttgaatcg atatgacttg aactataaga gtaggtttga
     6481 attaattttt acctgttctg aagaatggaa taaagaaaat attgcagctt ttctatcagg
     6541 agtggctgaa tatttgattg acaatagaca acctatttta agaggtgaaa ttattcaatt
     6601 acccagggta attatcgaag gtagcaaaat ggatgcactg tatgtttccg ccccatttta
     6661 tttcgacgat gattttcagg tctgttatgg cgaacactac aatatcgttt tccctttgct
     6721 tgtccccttg tataaacagg aagccgaatg ggtggaaaaa aagggttgga atgcttttga
     6781 gcagttcttg ctggataatg aagttggcaa cctttcggat atgaatcgga aaccgtttac
     6841 ttggtaagga ttttcttaaa tttaatcaaa gtgccgtctg aaaccgtgtt taattttcag
     6901 gcgaccttcc ccttatttta aacaggatat tgggaactaa gttcttcaaa aatcctacac
     6961 ctgctccttc cacggcagca ccttggtcaa aacggcagac ggctgaaaag caaacaccgt
     7021 ccgtcgtgtt gccgtttgcg gatgagtacg ggtcaacccc aatgccgccg aaaccgtcga
     7081 agccgccttc aacattgccg ccgccaaagc cgcaaagttg gcaaaaacgg taaaaccggg
     7141 gagataaaag ccgatggcag gaaagtaaat gtgaggatag acagtacgga ggcagacctg
     7201 ctttatccgg cagggcaata agaaaacaaa aattagatat ggaaaacgat tgtgaagatt
     7261 aaaccattac aattttctaa caataatcac agattttatg tggacaaaat ttgtgttacc
     7321 gaacaagatg tgaacatttt ggcatccgac cgaaattatg aattaaatat tgaaatattt
     7381 attgacgata taattcattt tcaaataaca gatgaatctt ataaagtaaa attttcagaa
     7441 tatttatttg aaaataaaga aaaaaatgat tgggatagaa atcctgctat aaattatttt
     7501 ttcgagataa tagatgatag ttatatggac tggttgaaag aagaaagttt tgattttttt
     7561 gaaaagaaat attataaggc ttatattttc ttttttagcg attctgtaat agaagttatc
     7621 agctcgacag aacctgtatt ttattcaaaa taacaaatta tcaaacaaag ctctgattaa
     7681 aaacccaaca atcaaaatac cgtctgaaac gatattcggc tttcagacgg tatttttgac
     7741 acaaagcagg taaccaaagg agtgtttgac ggaaaaggag aagctaaaat accggatgta
     7801 tcggttggga agcaatggat aaaggtaaat aattatgtgg aaaattagta aagaaaattg
     7861 tgaagattta ggatttgcaa tagtctgtat gttctatgat gctattaatc tttctgaatt
     7921 taaattatgg ttggatatag ttgtcagaga tattcctatt gatacaattc cattgtatat
     7981 ttttgatttg attgattttg ataagagtat aggggaaatt tatgatgtaa ttggagtcgt
     8041 taattatggt tacatttcaa atgatcaaaa aaatgcatta acgggcattg ccttcttaag
     8101 ggggatagat gtctatgatc cgcctatttc aaaagaaaaa gcattaaaag ccttagagaa
     8161 atatcctgaa atttatcaga ggtttcagca tttctttcct tttgtagagc ttccgctttt
     8221 ttaaaagaca atatgccgtc tgaaaagttt tcagacggca ttttttattt cttccagtag
     8281 gcgggggtga agaggatgaa gacggtgaag atttccagcc tgcccaagag catggcggta
     8341 acgcagatcc atttctgcat cacgtccaaa ccggcgtaat tgccggcggg cccgacttcg
     8401 cccaggccgg ggccggcgtt ggtgatgcag gcgatgacgg cggtgaaggc ggtggtaaat
     8461 tccataccgc tcgccatcag caggaagctg aagaggacga cggtcataaa gtagatgaag
     8521 atgaaggaca taacggtcag cgcgaggcgg tcgggtatgg ccttgccgct gattttgacg
     8581 gtgcggacgg ctttggggtg cagcagcacc atcatttcgc gcaggctgaa tttgaacagg
     8641 acgagggcgc gtatggtttt gatgccgccg ccggtcgagc cggagttggc gaggatgttg
     8701 gcgaggaaaa acatccacag ggaaatcagg agcggccatt gtgcgaagtc ggtgttggcc
     8761 agcccgtttg ccagtccgat ggagacgaag ttgaaggcgg tgtagcgcag ggattcggta
     8821 aaaccggcgt aatagccggt gtgccacagg tacagggcgg cggcaaggat gctgccggag
     8881 agcagcagca gcatcgtccg gcattcttcg tctttccaat aggttttgag gctgcggctg
     8941 ttgagggcgg cgaaatggtt ggcaaaattg atgccgccga caatggtgaa aacgatgatg
     9001 accgcttcga tgagggggga gttgtaataa gctatgctgg catcgtgggt ggaaaacccg
     9061 cccagcgaga gggtcgccat cgcgtgacag acggcatcga accagcccat cccggcaaaa
     9121 tgcaggcagg ctgccgcgag gatggtgatc agggtgtagc cgaaccaaag ttttttcgcc
     9181 acttgggaaa tgcgcggcga cattttgctt tctttgtcaa tgccggggat ttcggctttg
     9241 aataactgcg tgccgcctac gccgagcata ggcaggatgg cgacggcaag gacgatgatg
     9301 cccatcccgc ccagccagtt gagcatatgc cgccaaaagt tgacggaggg ggcgagcccg
     9361 tcgacgtggg ggatgacggt cgcgccggtg gtggtcagtc ccgacatcga ttcaaaaaat
     9421 gcgtcggtaa agcccatatt cgggaaatac aggtacatcg gcatcgcagc catagcggca
     9481 aacgccagcc acaacatcag gacgagggta aagccgtcgc gcgggcgcag ttcgcgcctg
     9541 aaccggaggg tggcgagccg gacgatgcac gagccggaaa gggtaacggt cgcggtggtg
     9601 gcgaaagcgg tgtacgcgcc gtccgaaaag gcgtaggaga gggcggcggg tatcagcagg
     9661 ataaaggaaa acagcatacc cagtcgggag aggacgtggg cgatgggcag gattttgtgc
     9721 atagtggggc ggtccgttat tttgcgaagc ttttccagtc tatgccgccg gcggcttgga
     9781 cttgggtaat ttcttccgtt tcgaggttga cggcggtcag ctgtccgccc cacagcgcgc
     9841 cggtgtccag cgagatgacg ttgtcggcat tcgtgtagcc cagcgaggac cagtgtccga
     9901 agatgatggt gcggtcgagg ttttgccggt cgggggcttt gaaccacggg cgcaggtaag
     9961 gcggcatttt tttcactgtg gatttgtagt cgaaatccag ttcgttttta aaggtcaggg
    10021 cgcgcatccg cgtgaaggcg ttgacgatga agcgcaggcg ggcatagcct ttcaaacctt
    10081 cgtcccacgc ggccggtttg ttgccgtaca ttttggagaa gaatttgacg tattttttgc
    10141 cgcgcagttc ggcttcggct tctctagcga gcgattcggc tttggttatg cgccattgcg
    10201 gcaggatgcc ggcgtgtacc atcacgcggc tgccctcgcg tatcaacagc ggttgcgcac
    10261 gcagccagtc gagcattttt tttccgtcgg ggtgtttgag tatgggttcg attgtgtcgc
    10321 tgcgtttggg cgcaccttcg ccgcagccga cagcgagcag gtgcaggtcg tggttgccga
    10381 ggacgatttg cacactgttt tcgtgccgga tgcagaattg cagcgtttcg agggatttcg
    10441 ggccgcggtt gacgatgtcg cccgtcagcc agagggtgtc cgtgccgtgg ttgaaaccga
    10501 ttttgccgag cagcgcggtc agttcgtcga aacagccttg tatgtcgccg attgcgtaat
    10561 gtgccattgc agatgttgtg aagtgggaaa gtgttgcggt tcggacggca tggttttgaa
    10621 atatcatgca gtccgaacgt ggaattatgc gttcaaaacg aggacggctt cggcttcgac
    10681 ctgcacgcct ttgggcagcg aggcaacgcc gacggcggcg cgggcgggga agggctcggc
    10741 aataaattcc gccatcactt cgttgaagac ggcgaaattg cccaagtcgg tcaggtaggc
    10801 gttgagtttg acgatgtcgg ccagcgtgcc gcctgccgct tcggcgatgg cttgcaggtt
    10861 ttggaacact tggcgcgctt cggcgcggaa atcgccgttg ccgacgacgg tcatcgtggc
    10921 gggatcgagg gggatttgac cgctcatgta aacggtgtcg cctgctcgga cggcttggct
    10981 gtacgcgccg atggcggcgg gggctttgtc ggtgtggatg atggttttgg acatttcgga
    11041 ttcctcaaaa aatagggcgg cagaagccgc agcattcggg attatcgtac aaaaccgccg
    11101 gcttgtgtag ttgcggtggc agaaaacaaa accgccgaag gctcggcggt ttgcagaata
    11161 aggcgcatat cagaatttga cgcgcacgcc gacggacagt tcgccggaac ggacgttttt
    11221 gacagtgttg actttgccga tgtagttgta gcggtagctg gcatccaaat cgacattcgg
    11281 ggtaacggca tagcttacgc ccgtcaatac gccgaggccg atggaggttt ggctgaagct
    11341 gtcgctgccg cccaagtcga cggaggcgcg gttgaggctc aagcgcgcgc cgagatacgg
    11401 tttgacgggc gattgggtgt cgaagtcgta aatggcggac gcgccgatgc tgtaaagttt
    11461 gaaatcggtg gatggggctt tatagttttt gtagcgcgtg taatcgacgg cgaagcggag
    11521 gtcgttgatg cggtagcctg cggagatgcg cgggctgaag cctttggcag aacctaaaga
    11581 gcttgaggct tttgcgtgtg cggcatcggc ttggacgtaa aagccggatg cgccttccgc
    11641 cagtgcggcg gccgggagag cgagggcaat cagtgcggca agtgcttttt tcatattttg
    11701 gttcctttat ggtcagttag aaaaattgtt aagaatccgt taaagaatcc tgctgtatta
    11761 tacttaaatt ttctttttgc atcgtaatat tttcaatact tcaagatacg tagcggtatc
    11821 cggctgcttt gccgacggca aagccgttaa cccgcgcgtt gcctttaaat ggtggcggcg
    11881 gcatcacgcg gcggatgggt gaaacttgca aacggtttgg aaaaaacagc ggtatctgtc
    11941 ggattgttgc aggtgcaggc atacggtttt gtgtgcgtct gtgccttaag cgtcggacat
    12001 ttccggcggc ggctgtgccg tctgaaacgc ccggcggggg atgcggctgc gttttccatc
    12061 gataagcata ttttccggac gcgttcgggg cgggttttcc cgggcggccg ccgatttgtt
    12121 tgcgc
//
LOCUS       DAXHKX010000039        11863 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHKX010000039 DAXHKX010000000
VERSION     DAXHKX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.039"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            64..693
                     /locus_tag="ACFMWZ_000899"
     CDS             64..693
                     /locus_tag="ACFMWZ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC5934883.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVEPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            1100..2194
                     /locus_tag="ACFMWZ_000900"
     CDS             1100..2194
                     /locus_tag="ACFMWZ_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC5934884.1"
                     /translation="MQNDAPQHVETSTESTPEGTVESESTPKEAGEEIKPAPKEDTSE
                     GKEAKETDPTQPSSTSESQDGTEENAVSEDTGMQASPPSETTEHTEPAMNAEDANPPS
                     KAENSDEVGQGGMQADEASGRQDAEEGLQGDDPTAHPPQDQTPQSAADNQPKTGVFGE
                     KYFDGTGEITTLVIGGETIDLTKADEPVSRKNHPGLMSDSDFEKVMVSQGQYQHLIYG
                     AAKLQDGTYTLFVQGKPTENLPTRMMAKYQGKVLHFVGKYKDNWFNNSAYRAAGTFTA
                     DVDFSRQTLTGKIDTKDPSGMGMQEFTASIDGSGFQGKWKSDNDQGDISGKFYGQDGA
                     EMAGRYDYKNQDNRKDGFGVFAGKKEADAR"
     gene            complement(2384..2512)
                     /locus_tag="ACFMWZ_000901"
                     /pseudo
     CDS             complement(2384..2512)
                     /locus_tag="ACFMWZ_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            2576..2863
                     /locus_tag="ACFMWZ_000902"
     CDS             2576..2863
                     /locus_tag="ACFMWZ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934885.1"
                     /translation="MMLTNQEVTGVKLGYRKAVLQELARDFGGSVHSIVLDAVDGHIE
                     RLKAKQAYDKRAMQAFQDYRETGLHITLNEFADWVEGLGGDESKDMPECRK"
     gene            complement(2869..2976)
                     /locus_tag="ACFMWZ_000903"
                     /pseudo
     CDS             complement(2869..2976)
                     /locus_tag="ACFMWZ_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2974..3297
                     /locus_tag="ACFMWZ_000904"
     CDS             2974..3297
                     /locus_tag="ACFMWZ_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC5934886.1"
                     /translation="MITRPAQSDLVRIYNFLNDLGAERQANTVMRLLKNSFVSTQGSP
                     SNGKPYDLSVAGETLQNVREVIVPYGKSGYSYLFWHDKSGKQILILTVRHFRENAYRL
                     SFPFL"
     gene            complement(3300..3563)
                     /locus_tag="ACFMWZ_000905"
     CDS             complement(3300..3563)
                     /locus_tag="ACFMWZ_000905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934887.1"
                     /translation="MSSVNISAIKKCRLKHFSDGIFNPLALLCGRFKSGHSILPYLHR
                     SNIPPAERNQSGSPVMFLKAVYPNVPQQSRNALPRTRESRQTK"
     gene            complement(3550..4026)
                     /locus_tag="ACFMWZ_000906"
                     /pseudo
     CDS             complement(3550..4026)
                     /locus_tag="ACFMWZ_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            complement(4159..5514)
                     /gene="yegQ"
                     /locus_tag="ACFMWZ_000907"
     CDS             complement(4159..5514)
                     /gene="yegQ"
                     /locus_tag="ACFMWZ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC5934888.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDILEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRSIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDAEGWATVEVKNR
                     FAIGDSLEIIHPSGNQTIKLEQMARKGQPVDVAPGNGIQVKIPNMRGKEKALIARVLN
                     P"
     gene            5839..6627
                     /locus_tag="ACFMWZ_000908"
     CDS             5839..6627
                     /locus_tag="ACFMWZ_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC5934889.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(6673..7272)
                     /locus_tag="ACFMWZ_000909"
     CDS             complement(6673..7272)
                     /locus_tag="ACFMWZ_000909"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC5934890.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            7400..8017
                     /gene="gmk"
                     /locus_tag="ACFMWZ_000910"
     CDS             7400..8017
                     /gene="gmk"
                     /locus_tag="ACFMWZ_000910"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC5934891.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            8076..8282
                     /gene="rpoZ"
                     /locus_tag="ACFMWZ_000911"
     CDS             8076..8282
                     /gene="rpoZ"
                     /locus_tag="ACFMWZ_000911"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC5934892.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            8374..10530
                     /locus_tag="ACFMWZ_000912"
     CDS             8374..10530
                     /locus_tag="ACFMWZ_000912"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC5934893.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLAVWHMDVQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKMVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(10597..11781)
                     /gene="coaBC"
                     /locus_tag="ACFMWZ_000913"
     CDS             complement(10597..11781)
                     /gene="coaBC"
                     /locus_tag="ACFMWZ_000913"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC5934894.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDEELSFPETSKDEAAMQIVERLAVYL
                     NK"
BASE COUNT         2960 a         2766 c         3222 g         2915 t
ORIGIN      
        1 atgcgggcaa gtgcggattt tgcccgcccc gcatccttat cccttaccga caaaggaaaa
       61 gctatgagcg aaaccgaaaa tcaagccctg acgtttgcca aacgcttgaa agcagatacc
      121 acggcggttc acgacagcgt ggataatctc gttatgtctg tcgaaccgtt tgtcagcaaa
      181 gaaaactaca ttaaattttt gaaactccaa tccgttttcc acaaggccgt cgaccacatc
      241 tataaagatg ccgaattaaa caaagccatt cccgagctgg aatacatggc gcgatacgat
      301 gccgtaacgc aagacttgaa agacttgggc gaagagcctt acaaattcga caaagaattg
      361 ccacacgaaa ccggcaacaa agcaatcggc tggctttatt gcgccgaagg ttctaatttg
      421 ggtgcggcgt ttttgttcaa gcacgcgcaa aaactcgatt acaacggcga acacggcgcg
      481 cgccacctcg ccccccatcc cgacggtcgc ggtaaacact ggcgcgcctt cgtcgagcat
      541 ctgaacgcct tgaacttgac tcccgaagcc gaagcggaag ccatccaagg cgcgcgcgaa
      601 gcctttgcat tctacaaagt cgtgttgcgc gaaactttcg gcttggcagc cgatgccgaa
      661 gcccccgagg gtatgatgcc gcacaggcat tgatatattt tacggtttcg cctcggctcg
      721 tgaagcggaa ccgtattttc tgccgcgtat gacggcattt ttcaaaccat cagatataaa
      781 taattatcat attttttatt aaaaatatgc cataggagat aattatgacg attcctttca
      841 aaccaagcct gcttgcaatg gcatccatcc tcgccctttc cgcctgtatt tcagggggag
      901 ggggtacacc tgtagtcaaa tcctcagaaa cgccgtcaaa taaagttacc ccccccccgc
      961 tgatacggta agccctgccc cgggtgctga tactgcaggc actgcttcgg gcggggatgg
     1021 aagagaatcc aaaacagaaa taccgcctaa agatacgcag ggagaagggg aaaccgcgca
     1081 ggcaggcgct gatggcggag tgcaaaatga tgcgccccaa catgtggaaa cttcaacaga
     1141 atcaacacct gaaggaacag tggaatcaga gtcaacacca aaagaagcag gagaagaaat
     1201 caaaccggca cctaaagaag atacatcaga aggaaaagaa gctaaagaaa ctgatccaac
     1261 acaaccttct tcaacttcag aatctcaaga tggtacggaa gaaaacgctg taagcgaaga
     1321 tacggggatg caggcaagtc ctccttctga aacaaccgaa catactgagc ctgcgatgaa
     1381 tgccgaagat gcaaacccgc cgtcgaaagc cgaaaattct gatgaggtgg ggcaaggcgg
     1441 gatgcaggct gatgaagcaa gtggcaggca ggatgcggaa gaggggttgc aaggcgacga
     1501 cccgaccgcc catcctcccc aagaccaaac cccccaaagt gctgccgata accaacctaa
     1561 aacaggcgtg tttggcgaga aatattttga cggtacggga gaaatcacga cgttggtgat
     1621 tggtggggaa actattgatt tgacaaaagc tgatgagcca gtatcacgta aaaatcaccc
     1681 cggtttgatg agtgatagtg attttgagaa ggtaatggtt tctcaagggc aatatcagca
     1741 tctgatttat ggggcggcta aattacaaga cggtacatac accctttttg tacaaggtaa
     1801 gccgacagaa aatttgccga caagaatgat ggctaaatat caaggtaagg tgctgcattt
     1861 tgttggaaaa tataaggata attggtttaa taatagtgcg tatagagccg caggaacatt
     1921 tacggcagat gttgatttct cacggcaaac cttaacagga aaaattgata ccaaagatcc
     1981 ttcaggtatg gggatgcaag aatttactgc aagtatcgat ggaagtggat ttcaaggtaa
     2041 gtggaaatcg gataatgatc aaggcgatat ttccgggaaa ttttatggtc aagatggcgc
     2101 ggaaatggca ggcagatacg actataaaaa tcaagataat agaaaagacg gcttcggcgt
     2161 atttgcaggc aaaaaagaag cagatgcccg ttgatattta tcctatgttt cacagtcggg
     2221 aacagttcgg gtttccttcg gacagggtat cggtcaacac tttgatgtgt tgacctgcct
     2281 gcagaagcga tcctaattga accatcatct gtattactga tttgatttat ggactatgcc
     2341 gtctgaaaaa tgtttcagac ggcatttttt ggttgccgat atgttaacgg acgacattgg
     2401 cgcgcataac cacgcggacg tggaacgagc ctgcctgaca atcgtattgt ccgccttgca
     2461 gcggctgctt gtcgaagtag ctctggaagc cggtaacact gcgtccgcaa aggtggcggc
     2521 agatgccgca ttgtgtttat aatgccgtgt cagacgtttg atttatggga aaataatgat
     2581 gcttaccaat caggaagtaa ccggtgtcaa actgggatac cgcaaagccg ttttacaaga
     2641 gctggcccgg gatttcgggg gcagtgtgca cagtatcgta ttggatgcgg tggatggtca
     2701 tatagaacgc ttgaaagcca aacaagccta cgataagcgg gcgatgcagg cttttcagga
     2761 ttatcgggaa acaggattgc atattacgct taatgagttt gcggactggg tggagggctt
     2821 gggtggcgat gaatcgaagg atatgcccga atgccgcaag tgaagttgat atagtggatt
     2881 aaatttaaac cagtacagcg ttgcctcgcc ttgccgtact atctgtactg tctgcggctt
     2941 cgtcgccttg tcctgattta aatttaatcc actataatta cacgccccgc tcaatctgat
     3001 ttggtcagga tttataattt tttgaatgat ttgggtgcgg aacgtcaggc aaatacagtg
     3061 atgcggctgc ttaaaaattc ttttgtttct acacaaggca gcccgagtaa tgggaaacct
     3121 tatgatttgt cggtggcggg ggaaactttg caaaatgtaa gggaagtaat cgtgccttat
     3181 ggcaaaagcg gttatagcta tttgttttgg cacgataaat caggtaagca aatattgatt
     3241 ttgacggtga ggcatttccg tgaaaatgcc taccgcctga gctttccttt tttataacgt
     3301 catttggttt ggcggctttc ccttgtgcgg ggcaatgcgt ttcggctttg ttgcggcacg
     3361 ttcggataaa ccgccttcaa gaacataacc ggactgccgc tttgattccg ttccgcaggc
     3421 gggatgtttg aacggtgcag atatggaagt atggagtgcc ccgatttgaa tctgccgcac
     3481 aataaggcaa ggggattaaa aatgccgtct gaaaaatgtt tcagacggca ttttttgatt
     3541 gccgatatgt taacggacga cattggcgcg catgaccacg cggacgtgga acgaacctgc
     3601 ctgacagtcg tattgtccgc cttgcagcgg ctgcttgtcg aagtagctgt ggaagccggt
     3661 aacgctgcgt ccgccgagtt tgccggcggt ctgctggaat ttgcgggcgg cattgatcag
     3721 ggcgcgttcg caggcctcgg agtcggattt gcctacgccg tttgctgcct gccgtgtaac
     3781 cagattcctg ccgtttgcga ccggtacgcc cccgccgccg aagctgacgg gaatcccgga
     3841 ttccaatacg ccggtggcgg tggccgcatt tattgccgcc tgaccgttgc tgcaacggta
     3901 aatgatgttt ttgccgcctc ctgcggtatc tccacggatg actttgcatg attctccggc
     3961 cggtgtgccg gcggatgccg tgtgtacgtt tttaggcgcg cggccggatt agcaggctgc
     4021 cagcatcagg gcggcggcag agagtatgaa gatttttttc atgtcgggta tccttgcgtg
     4081 ctgtgggaaa tcattggcat tgtacagtaa gattcaacaa aatgccgtct gaaaacgggg
     4141 ttcggacggc attgtggctc aggggttcaa tacgcgtgcg atgagggctt tttctttgcc
     4201 ccgcatattg gggattttga cctggatgcc gttgccgggt gcaacatcga caggctggcc
     4261 ttttcgggcc atttgttcca atttgatggt ttggttgccg ctcgggtgga tgatttcgag
     4321 cgaatcgccg atggcaaagc ggtttttaac ttccaccgtt gcccaacctt cagcgtcgat
     4381 ttcggtaacg tggccgacat attggctttg tttggcgatg gagtggccgg tcaggtagtt
     4441 ttgatagtct tgggtttggt ggcgttcgag gaagccgctg gtgtagccgc ggttggcgag
     4501 gccttcgagt tcgctcaaca ggctgtaatc aaacggacgg cctgcaacgg catcgtcaat
     4561 cgctttgcgg taggattgag ccacgcgtgc aacgtagtag agcgacttgg tacggccttc
     4621 gactttgagg ctgtccacgc cgattttggc gagtttctca accacttcaa tactgcgaag
     4681 gtctttggag ttcatgatgt aggtgccgtg ttcgtcttcc ataatcggca tcatctcgcc
     4741 cgggcggttg gactcttcaa tcaggaaaac tttgtcggcg tagggatggc gtttttgacc
     4801 gttgatgcct tcaaagtttt ggttggcttc ttcttgggct ttttcaaagt tgaaaccttg
     4861 cagaagctgg gcatcgcccg catcgctttc cgtagcgttg tggactttgt agtcccaacg
     4921 gcaggagttg gtgcaggtgc cttggttggg gtcgcggtgg ttgaagtagc cggagagtag
     4981 gcaacggccg gagtaggcga tgcacaatgc gccgtgaatg aagacttcga gttcgatgtc
     5041 ggggcattgt tggcggattt cggcgatttc ttccatactc aattcgcgcg acaggatgat
     5101 gcgttcgacg ccgatattct gccagaattt cacgccccag tagttggtgg tgttggcttg
     5161 tacggacagg tggatcggca tttccggcca tttttcgcgc acggtcatga tcaaacccgg
     5221 atccgccata atcagcgcgt cgggtttcat ggcaatcaag ggttccatat cggaaacaaa
     5281 ggttttgagt ttggagttgt gcggcagggt gttgacggta aggaagaatt ttttgttgcg
     5341 ctcgtgcgct tctttaatgc cttgctcgag gatgtcgagt ttggcaaatt cgttgttgcg
     5401 ggcgcgcagg gagtaacgcg ggctgccggc gtaaacggca tctgcgccgt aatcgtaggc
     5461 ggcgcgcatt ctttccaatc cgccggcggg caataagagt tcgggtgctt tcatcgtgtt
     5521 tccttttcgg ttgaaacccc gccctttagg gcggtagaat cagactttat ttgggagggg
     5581 tgtaaccctt tccgaatcag ggcaacatat agggcgacgc tttatgtgtc gtcctgtgtg
     5641 ttgaaacatg atttgtatgc tttgtaaaaa tgtttcagac ggcattgctg caaatgccgt
     5701 ctgaaaaggg tttgaggatg ggcggattat ccgccttttc gtgttcgcgg tcaattttgc
     5761 cgggaaattt ataaggattt atcaagtatt tgcctgattg ggatacaatg cggcagtttg
     5821 aatgcgagag agaatgtgat gaatcctaaa atcgtgtttt tcgatattga cgatacgctg
     5881 taccgcaaat atacggatac cttgcgtcct tccgtgaaaa cggcggtggc ggctttgcgc
     5941 ggcaaaggca tattgacggc gttggcaacg gggcggtctt tggcgacgat tcccgaaaag
     6001 gtcagggaca tgatggcgga aacgggcatg gatgccgtgg taacgataaa cggacagttt
     6061 gcgctgctgc acggaaaaac tgtgcgcgaa gtaccgatgg atgccggttt gatgggcagg
     6121 gtttgcgcgc atttggatgg cttgggtatg gattatgcgt ttgtcggcgg agaggggatc
     6181 gccgtgtccg cgctgtcgga atgcgtgtgc cgcgccttgc agcatatcgc cagcgatttt
     6241 tttgccgata aggattattt ttcaagcaaa ccggtgtatc agatgctggt gtttgcggag
     6301 gaaaacgaaa tgccgctctg gtcggatatt gtggaacagg aaggcttgaa aacggtgcgc
     6361 tggcacgagg aagcggtcga tttgctgcct gcgggtgcgt cgaaaacaga cggcatcaga
     6421 agcgtggttg aagcattggg tttggaaatg gcggacgtga tggcgttcgg agacggtttg
     6481 aacgatgtgg aaatgctgtc agaagtcggg ttcggcgtgg caatgggcaa cggggaacag
     6541 gcggcgaaag aagcggcgaa atatgtttgc cccggcgttg atgaagacgg cgtgttgagg
     6601 ggcttgcaag atttgggcgt gatttgaacg cataataaca accctgccgg tttcagacgg
     6661 cagggtcggt tttcagccct tcatacagcc ttcgttttga agcagggtaa ataagggcgc
     6721 gccgcttgca cggatattct tgccgccttg aaggtcggta aattccaaaa tagcggcagc
     6781 ttcgacaatt tctccgccga gtttgcggat cagttccagc ccggcaagca ttgtgccgcc
     6841 cgtggcaacc aaatcatcga ccagcagcac gcgcgaaccg ggtttgacgg catcggtgtg
     6901 gatttccacc gcagcttccc cgtattcgag cgcgtagctt tgcgatacgg tttcaaaagg
     6961 cagcttgcct tttttgcgga tggggacaaa accgacgttg agatggtagg cgagtgccgc
     7021 gccgataatg aagccgcgcg cgtccaaacc ggcaacgatg tcgattttct gatccatata
     7081 gcggtaaacc aataaatcaa ccaaaagtcg gaagtattcc gcgctttgca ggacgggcgt
     7141 gatgtcgtgg aataagatgc ctttttgcgg ccagttttcg attttgcgga ttttgtcggc
     7201 aagcgcgccg acactcatag cttcgggatg aaccagcatt gcgtgttcca agtttgatgt
     7261 ttttaacgcc atattgtagc cgtcagcctg ccgcttgcca aatcaatcaa aacaatagcg
     7321 gcaaaatatg ggcatcggca aataggaaac cgagtgtccg ttgtgataca atcggctgga
     7381 aaatatacag gtaaaaaata tgtccgctta tcgaaaaggc aacatcttta tcatttcggc
     7441 cgcttccggc acgggcaaaa ccacgctggt gtcgcggctg ttggcaaacc ataacggttt
     7501 gcgcgtttcc gtatcgcaca cgacgcgtcc gccgcgtgaa ggcgaagtaa acggcgtaca
     7561 ttatcatttt gtttctaaag aagagtttga gtcgcttatc gcgcaggaag cttttttgga
     7621 atacgcagac gtgttcggca actattacgg cacaagcgcg gagggcgtga atgctttggc
     7681 ggcggcaggc tatgacgtaa ttttggaaat cgacgttcag ggcgcggcgc aggttcgcaa
     7741 cgcgctgccg gaagccgtcg gcatctttat cctgccgccg tctttcgatg tgcttgctgc
     7801 acgtctgaac ggacgcggga cggacagtcg ggaagttatc caaaggaggc tgtcgaaggc
     7861 aaggcatgaa atcgaacagt ccgtattgtt cgattttgtc gtggtcaatg atgacttggc
     7921 gcgggcggag gaggatttgc gccatattgt gaatgcctgc cgtctgaaaa ggtcgcggca
     7981 actgggattt attgcagatt tgttggaaaa ttcctaaaaa accgcgaaaa tacccagttt
     8041 cctattttaa atatttttga aagaaagcaa ataatatggc acgtattacc accgaagact
     8101 gtaccggaaa aatttccaac cattttgacc tgacattggt agcggcccgt cgggcccgcc
     8161 agcttgaaaa tggcaatacg ccgcttgtgg acgatgtccg caataacaaa ccgaccgtta
     8221 ccgccttaag ggaaatcgcc gccgggcata tcggtacgga actgttgacg cgcaataaat
     8281 aaattctgcc ggaaacgcac gccggaacac tttgccgccg tgcagtccga cggtttgaaa
     8341 tgaaaaacac atacataccg aaagccgtct gaaatgcccg ccccccaacc ttccgcccct
     8401 tacgaccccc tgaccgccga agcgcgtgcc ctgcttttcc ataccgcctc ctacctcaag
     8461 cccgaggaac aggcggagct tgaaaaagct gtcgcctatg cgtttcgtgc ccacgacggg
     8521 caaacccgca aaagcgggga gccctacatc acgcatccga ttgccgttgc gacacagctt
     8581 gcggtttggc atatggacgt acagggtctt tgtgcaggtg taatgcacga cgtattggaa
     8641 gatacgggcg tgacaaaagg ggaaatggcg gcggtgttcg gcaatacgat tgccgagatg
     8701 gtggacgggc tgtccaaact cgaaaaactc aaatttgaag atcatgcgga acatcaggcg
     8761 gagagtttcc gcaaactgat tttggcgatg accaaagatg tgcgcgtcat cgtcgtcaaa
     8821 cttgccgacc gcctgcacaa tatgcggacg ctcggttcga tgcgcccgga caaacgccgc
     8881 cggattgcaa gggaaaccct tgaaatctat gcacagattg ccaaccgtat aggtttgaat
     8941 aacgcatatc aagagcttca ggatttatcg ttccaaaacc tgcatcccaa ccgctacgag
     9001 actttaaaaa aagcgatgga caagagccgg aagaaccggc aggacgttgt cggcaaagtc
     9061 ttgcgcgcat tcggccagcg gctggtaggc gcgaatatag aggccaaaat caaaggcagg
     9121 gaaaaaaacc tgtacggcat ccatcagaaa atgatggcga aaaagctgcg ctttgccgag
     9181 gttatggata tttacggttt ccgcgtcatt gtcaacagca ttccagcctg ttatgccgca
     9241 ctcggcgcat tgcacaccct ctatcagccc aagcccgggc ggttcaaaga ctatatcgcc
     9301 attccgaaaa gcaacgggta tcaaagtctg catacgactt tggtcggccc ttacggcttg
     9361 ccgattgaag ttcagatacg taccaaggaa atggatgctg ttgccgaagg tggaatcgcc
     9421 ggacattgga gctataaatc atattctaag atggtcgatc aggcggtgct tcacacaaac
     9481 cggtggctga aaaatatctt agatttgcag gcaagcagtg ccaatgccat tgagtttctc
     9541 gaacacgtca aagtcgattt gtttccgaac gaaatctaca tccttacgcc aaaaggaaaa
     9601 atcctaactt tgcccaaagg ggcaacgcct gtcgattttg cttatgcggt gcataccgat
     9661 atcgggcaca aaaccgttgc cgcacgtatc aacaatatca tgatgccgtt gcgtacgaag
     9721 ctcaaaaccg gcgattccgt cgaaattatt acatccgaac atgccaaacc caatcctgca
     9781 tggttgaatt tcgccgtgtc aggcagggcg cgcagtgcca tacgccaata tattaaaaac
     9841 cttaaccggc acgatgcggt cgttttggga gagagcctct tacaaaaagc cctgtccagt
     9901 ttgctgccca aagatgtcct gctttcagac ggcattaaag aaaaatacct tgccgatctc
     9961 aacgacaaac agacttcatt tgaggaagtg ctgtacaacg tcggtatggg gcataccctg
    10021 cctgtttatg tcgccatgca cattgccgag ttggcagggg agcatttcgg cagcgaggtc
    10081 aggctcagtt ccattaaagt tgatggtcag gaaagcgggc atattcattt tgcagagtgc
    10141 tgccatccgg ttcccggcga ttccatccgt ttgctgttgg ttaagggaaa aggcatgatt
    10201 atccataggg atacctgccc gacgttgtta aagtccgatc ccgaacagca gctggatgca
    10261 gactgggaaa atatgaacgg gcagaactac cgtgtcgggc ttcaagtcca atcggaagac
    10321 agccacggct tgctggcatt aatggcgcaa gcgatttccg attccggtgc ggacattgag
    10381 tcggtcgaaa caccgtctaa atcccagtcg ggaacggaag gttttgtcga attcaaattc
    10441 ttattgaaag tcaagaatct gaatcaattg aatcagatta ttcaaaatct gcatagtatt
    10501 ccatatatcc gcaaagtcat cagaagttga agcaggtaaa acgggttgcc ttttgattaa
    10561 caggcaaccc gctttatggt ttatccgttc aattgcttat ttgttcaaat atacggcaag
    10621 cctttcaaca atctgcattg ccgcttcatc tttacttgtt tcgggaaaag acagttcctc
    10681 gtcgtccccg ataatggtaa tccggttggt cggtttgccc atcgcgattg aaacatcatt
    10741 ggcaacgatc actggtattt ttttcttaat acgtttttct cgcgcatatg ccattacatt
    10801 ctccgtttca gcggcaaaac cgatgcagaa cggcgggttc ggcaatgagg caatagaagc
    10861 caaaatatcg gggttctcat ccaattcgat ggataatggt ttggcatttt tatctttttt
    10921 gaatttttgc gtactcctgt ttttaacctt atagtctgag acggcggcaa cagaaataaa
    10981 agcatcttgt ttgtcgatta aacgatgcac tgcgcgatgc atatcttcag cgctgacggc
    11041 ttgaaccgta tcggatatgc cgaaaggcag cgcggtttga agctgtccgt gaatcaggct
    11101 gacttccgca ccggcggcac ggcacgcccg cgccaaagct acccccattt ttccgctgga
    11161 ggtatttgtg atgcccctga cagggtcaat ggcttcaaat gtcgcacctg cggtaatcaa
    11221 gattttcttg ccccttaaaa ttttcggtgt ccacaagtcc ggaagcaaat ccagcaattc
    11281 ggcaggttcc ggcatccttc ccatgccatt ttctccgcaa gcctgttcgc ccaagcccgg
    11341 catatagaca gtaatgccgt ctgaaaccag ttgtgcgata ttccgttggt tggcagggtt
    11401 gagccacatt tccacattca tcgcgggagc gatggcaagc ggacatttcc gtgcggctgc
    11461 cagactggtc agtaggttat ctgccacgcc gttacagatt tttgccacgg tattcatact
    11521 tgccggcgca atcagaaaaa catccgcatt ccgggtcagg ttgatatgtt ccataccgtt
    11581 tgaaccgttg ccgccgtgcg tgtcggtcag gacaggattg ccgcttaaag cctgaaaagt
    11641 cagcggagaa acaaattcag ttgccgagcg gctcataacc accgtaaccg aatgcccctg
    11701 ttttttcagc agacgaacca actcgcaaga cttatacgcc gcaatactgc ccgttacacc
    11761 taaaagaata tgtttgccca ttttgacaat ttctaattac gaagaaaaaa cagcagccat
    11821 tctataacaa agtgtggata cattagatac cgtttggata caa
//
LOCUS       DAXHKX010000040        11749 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHKX010000040 DAXHKX010000000
VERSION     DAXHKX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.040"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..337
                     /locus_tag="ACFMWZ_000914"
     CDS             <1..337
                     /locus_tag="ACFMWZ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC5934895.1"
                     /translation="FFDTEPDRGKIEHCACLAMIHEEISAMPMGYETLIGDMGSALSG
                     GQKQRIVLARALYCEPKILFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVE
                     SADRKMSLG"
     gene            complement(358..1584)
                     /locus_tag="ACFMWZ_000915"
     CDS             complement(358..1584)
                     /locus_tag="ACFMWZ_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC5934896.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1599..2024)
                     /locus_tag="ACFMWZ_000916"
     CDS             complement(1599..2024)
                     /locus_tag="ACFMWZ_000916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934897.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGATVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2129..2788
                     /gene="queC"
                     /locus_tag="ACFMWZ_000917"
     CDS             2129..2788
                     /gene="queC"
                     /locus_tag="ACFMWZ_000917"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC5934898.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2816..3331
                     /locus_tag="ACFMWZ_000918"
     CDS             2816..3331
                     /locus_tag="ACFMWZ_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC5934899.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDHVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            3338..3760
                     /gene="queD"
                     /locus_tag="ACFMWZ_000919"
     CDS             3338..3760
                     /gene="queD"
                     /locus_tag="ACFMWZ_000919"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC5934900.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYGRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            3869..4225
                     /locus_tag="ACFMWZ_000920"
     CDS             3869..4225
                     /locus_tag="ACFMWZ_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934901.1"
                     /translation="MNTTSSMTQEEGIIAESIDVINKFVQFLLKLYDDFGIDGMHDLV
                     DPDLDTLESIVKNLQQEVDKLPISPNDFSLENKKISLAQGLLYAQSMITNVRNKDTEE
                     CSRNRSMLKNNQSSLY"
     gene            4245..4370
                     /locus_tag="ACFMWZ_000921"
     CDS             4245..4370
                     /locus_tag="ACFMWZ_000921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934902.1"
                     /translation="MKSMDIRFARIAALTKQLAAIQRQIEKRAYKAKAATMKKVA"
     gene            4475..4846
                     /locus_tag="ACFMWZ_000922"
     CDS             4475..4846
                     /locus_tag="ACFMWZ_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC5934903.1"
                     /translation="MKLLSTILVLLVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGAMVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4843..5481
                     /locus_tag="ACFMWZ_000923"
     CDS             4843..5481
                     /locus_tag="ACFMWZ_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC5934904.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5816..5892)
                     /locus_tag="ACFMWZ_000924"
     tRNA            complement(5816..5892)
                     /locus_tag="ACFMWZ_000924"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5856..5858),aa:Pro,seq:cgg)
     gene            complement(6120..7205)
                     /gene="nagZ"
                     /locus_tag="ACFMWZ_000925"
     CDS             complement(6120..7205)
                     /gene="nagZ"
                     /locus_tag="ACFMWZ_000925"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC5934905.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHSNPEAVTRLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLIPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAALASPQDTAGGVKVGEAF"
     gene            complement(7262..8782)
                     /locus_tag="ACFMWZ_000926"
     CDS             complement(7262..8782)
                     /locus_tag="ACFMWZ_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC5934906.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETTVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVDPSLIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8984..10483)
                     /locus_tag="ACFMWZ_000927"
     CDS             complement(8984..10483)
                     /locus_tag="ACFMWZ_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC5934907.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(10621..11250)
                     /gene="nth"
                     /locus_tag="ACFMWZ_000928"
     CDS             complement(10621..11250)
                     /gene="nth"
                     /locus_tag="ACFMWZ_000928"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC5934908.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(11296..11709)
                     /locus_tag="ACFMWZ_000929"
     CDS             complement(11296..11709)
                     /locus_tag="ACFMWZ_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC5934909.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2833 a         3068 c         3223 g         2625 t
ORIGIN      
        1 ttttttcgat acggaaccgg atagaggaaa aatcgaacat tgcgcctgcc ttgctatgat
       61 tcacgaagaa atctccgcca tgcctatggg ctatgaaacc ttgatcggcg atatgggcag
      121 cgcactgtca ggcggacaaa aacaacgcat cgtattggcg cgggccttat attgcgaacc
      181 gaaaatccta tttttagatg cagcgaccag ccatttggat attgccaatg aaaaagcagt
      241 caatgcaaac ttgaatggct tgtctatcat aaaaattatg gcggcacaca gaaaggaaac
      301 ggtggaatca gcagatagga aaatgtcttt aggataaaaa tacagtttca aaaatactca
      361 agattgctgc tgttttttcg cctgagcgtc aaactctgcc agcgtcatgt tcaaagtctg
      421 caaacacggt gtcattaccg catcgacagc ttggttcaca tgatcccttt ctacaggcaa
      481 cgggcggtac acgaagagct tgaagagttc gttcaactca atcgaatccg cccccgtttt
      541 caaaacccag ccctgtctgc cggaatagat atagccatat cgcgccaact tttccaagag
      601 ttcgcccaac tcgtcgtagc ccatattgat atgccgtctg aactcctgaa caggcaaggc
      661 tttgccttct ttttgcgccg catccagaag cagcaggatt ttcaacacgt cgtcaaaccg
      721 tccgcgcgag tcgaagcccc tgcggaacgc ttctccctgc cagtaggaga gtgaagaagt
      781 cagcaccgcg ccgcccaaga ccagcgtcca caacaggttc agccacaaca gaaaaaacgg
      841 cacggcggca aacgcgccgt aaatcgagcg gtagccgtcg aaattgccca tataccaagt
      901 gaagagggag cgcgcggttt ccagacaaaa cgctgttgcc aaagccccga caaacgcctg
      961 ccgcgcggga acgaagcggt ttggcacgaa gcggtacagc ccccacagca aaagcgtcat
     1021 gaaggtcagc gtcgccgccg ttcgcaacgc gcccgaccac tgcggcgcac ctgaggcaag
     1081 cgcggcatcc tgtaccgagc cgaccataaa ggaaatgccc acgcccaaag acagcggccc
     1141 gaacgtcagt aaagcccaat agacgagaaa ctgcatcatc cacggacgct gggaattgac
     1201 ccgccagatg cggttgaacg tattgtctat cgtccgaatc agcatcagcg aggtaacgac
     1261 cagcatcacg ctgccgattg ccgtcagccg gttcgcctgc tcgcggaacg cattgatata
     1321 gtcgaacacc atgtccgcgc cctgcggcac aatggtttgg ttgacgaagg agacgaacga
     1381 atccgaccag cggtcgaaca cggggaaaat cgaagcgacc gccaccatca cggtcagcac
     1441 ggggacgagt gccagcagtg tcgtaaacgt catgcttgcc gccgcctgcg gtacgcgttc
     1501 ttcatcaaag cggcggacga cgaaccatgc aaacgcacag attttattgt ctgccaaacc
     1561 ttgcaaacgt tgtaaaaagg tcataatttc ttgcccggtc agtaagttgg gcattgatgc
     1621 ccgatgttat agccaatttt gccgtcagga acaaatgcct gaactgcggc tgtttcagac
     1681 ggcatcggaa caactgttat gccgtctgaa gaccgaacca ttttaacgga atccgcccat
     1741 gaacccaaat ccccctcaaa atcctcgtcc tctactattt ccaaaacggt agcacccgca
     1801 atcccgcccg ccgaatcgct cgcggcatcg acagcgttga aggttgcgaa gccgtattgc
     1861 gcaccgtccc caaagtctcc gccgtctgcg aagccgtcaa aaaagatatt cccgacagcg
     1921 gctcccgtcc tgaccgccga agaaaacaat atcgccttcg cacaaagcaa acgcttggcg
     1981 gaactcgcca tcaagtcggc ataagccgcg tgttcagacg gcatggtgtt cagatgccgt
     2041 ctgaacgcgt ttgcctgtat aatccgcgtc tttactgtcc aacttcgcgg ttcgcaaacc
     2101 tcccgcgtca ccaaaactag gattcgatat gtcaaaccaa aaagccttgg tcatcttttc
     2161 gggcggtcag gattcgacca cctgcctgat tcaggcaatc caaacctacg ggcgcgaaaa
     2221 cgtccaagcc attactttcc aatacgggca acgccatgcc gtcgagctgg aacgtgcccg
     2281 ctggattgcg caggatttgg gcgtcaaaca aaccgtactc gacttgagcc tgatgcggca
     2341 gattacgcac aatgccctga tggacgacac cgccgccatc gaaactgccg aaaacggcgt
     2401 tccgaatacc tttgtagacg gccgcaacgc gcttttcctg ctctatgccg cgatttacgc
     2461 caaagggcag gggatacggc acatcatcgc gggcgtgtgc gaaaccgact tctccggcta
     2521 tcctgactgc cgcgacgtgt tcgtcaaatc catgaacgtt acccttaatt tggcgatgga
     2581 ctatgatttc caaatccaca cgccgctgat gtatctgacc aaggcgcaaa cgtgggcgtt
     2641 ggcggacgaa atgggcgtgc tggactatat ccgcgagcaa acccacacct gctataacgg
     2701 catcgtcggc ggctgccgcg aatgcccgag ctgtatcttg cgcgaacgcg ggctggcgga
     2761 atatctggaa agtaaaaagg ccgtctgaac acgcgcaaac cataaggaat acgatatgcc
     2821 caagctccat atgttttacc tcggcggcaa tgccggcagg tcgaatatcg aagtgcacga
     2881 catccaattt gccgtgtgcg acgactaccg tgaagccgtc cccgcactca aagccgcgtg
     2941 gttcggcgat gcggacaaaa tccacatcga cggctggcag gttgtcgaat gggcggacgg
     3001 ttacgacatc gccgtatccg aaacgcccaa aacggaaatg ccgtctgaac acgccccgcg
     3061 cctgtatttt gccaatgtcg gcggttatcg cgcggggcag cttgccgagg cgcacgcgtt
     3121 cgggctgttc gccgccgcca cgcctgccga agccaaacaa aaagccctgc aaaccctgtt
     3181 gaccgaccat gttcagcaac ataaagacaa cttaaaagac gtggacaacc tgcttgcgct
     3241 cgaccgcatc ggcaatttcc atatccgcct gaccccgaat ccgcacggca aacccgccga
     3301 aatcggcttt caaggctatt tgcccatttg agaacccatg aaaatcacca aaatcttcac
     3361 cttcgactcc tcgcatatgc tcgacgggca tgacggcaaa tgccaaaact tgcacggaca
     3421 tacctacaaa ctcgaaatca ccgtttcaga cggcattatc aaaggcggcg cgaaagacgg
     3481 tatggtgatg gactttaccg acttgaaagc cattgtcaaa caacacatta ccgacccctt
     3541 cgaccacgcc ttcatctacc acggcggcaa cagccgcgaa tctcaaatcg ccgcactctt
     3601 ggaaggctgg aacatgaaaa ccctgcgcct gccctgccgc accactgccg aaaatatggc
     3661 ggtcgaaatg tacggccgtc tgaaaaacgc ggggctgaac gtgtgccgcg tcaaattgtg
     3721 ggaaacgccg acatcgtgtg cggagtatga aggggagtaa tattgaaaca aattttaaag
     3781 atttgttttg acttgtcata taatcaattg taaaattcca tactttctaa aaaatcgacc
     3841 gtaatagaaa atttcaggca taataaccat gaatacaact agtagtatga ctcaagagga
     3901 aggcattatt gccgagagca tagatgtaat taataagttc gtacaatttc ttttgaaact
     3961 gtacgatgat tttgggattg atggtatgca tgatttagtt gatcctgatt tggatacttt
     4021 ggaatccatt gtgaaaaatt tacaacaaga ggttgataaa cttccgatca gtcccaatga
     4081 tttttctttg gaaaacaaaa aaattagttt ggctcaagga cttctctacg cacaatctat
     4141 gattactaac gttagaaata aagatactga ggaatgttct cggaatcgtt ctatgctcaa
     4201 aaataatcag tcgagccttt attaacaaca gcaaggagtt atttatgaaa tcaatggata
     4261 tccgtttcgc tcgcattgct gcattaacga agcaacttgc agctattcaa agacaaattg
     4321 aaaaacgagc ttataaagca aaagctgcaa ccatgaaaaa agtagcatga aaagttttgg
     4381 tagagaagcc atccataatg ggtggctttt ttgttaaata aaattggtaa agtttgttaa
     4441 atataaatct tagtgcaaat taaggtgctt acccatgaaa ctcctctcca ccattctcgt
     4501 cctccttgtc gccgtcgaac atttctacat cgcctggctt gaaatgacac agattcccag
     4561 cgaaaaagcg gcggaaacgt tcaagctgcc ttatgaattt atggaacaaa atcgcgtgca
     4621 gaccctgttc ggcaaccaag ggctgtataa cggctttctc ggcatcgggc tggtgtggtc
     4681 gcggtttgcc gcgccggata acgcggtgta cggcgcaatg gtactgtttc tcggcttcgt
     4741 cctgattgcc gccgcgtggg gcgcgttctc ttccggcaac aaaggcatac tcgtcaaaca
     4801 aggtttgccc gcatttttgg cagcggcggc ggtgttggcg gtatgaaaaa aatcaatgtt
     4861 gcccccgaaa atccccaata ccgcatcgtc gaaatctttg aaagcctgca aggcgaaggc
     4921 tggaacacgg gcatgcccgc cgttttcgtc cgcttgggca aatgcaacct cgcctgcggc
     4981 tggtgtgata ccgattattt gacattcggc atgatgagcc tgtccgacat cttaggctgt
     5041 ctgaaaacct atgccgcccg caacatcatc atcaccggcg gcgaaccgac catacagccg
     5101 catctcgata tgctgctgga cacgctcaag gcggaaggct atttcctctg tctcgaaacc
     5161 aacggactca atcccgcgcc gccgcaaatc gactacgtcg ccaccagccc caaagcctgc
     5221 tacgccgcca aatatgaaaa tagctgtatc gaaacagccg acgaagtgcg gattgttgcc
     5281 gatggtgatg tccttgcgtt ctgcgaaaac atggaacgca aaatccgcgc acatcattac
     5341 tacctttcgc cctgtgagca agacggtgcg atgaacatct acgacaccat ccgccaaatc
     5401 ggtattttaa acagtcgccc cgacgcatcc gtgcattggc agttgagcgt gcagacgcac
     5461 aaatgggcgg gaatagagta gtttaagcag tgtaactcaa agggacggcg tacggtttta
     5521 ccgatgtttg acatacgggg aaagtgtgcc gcttccgcgt gaaactgccg gcatttccgc
     5581 cgcccaatca ggacggagcc ttactgaata agatgccgcc gttgggtaca agcccggctt
     5641 cctaaattcc gatggtcttt tgaaccttgc cgataccctt tgtcagtgca cgcaaatagc
     5701 agggttaggg aaaacgaaat gccgtctgaa acagcattct gtttcagacg gcatttttct
     5761 gttgccgcca aaaggcaaaa ccgccccggc aatggatgcc gaggcggttt ggatttggtc
     5821 ggaatgagag gattcgaacc tccgacccct tcgtcccgaa cgaagtgcgc taccgggctg
     5881 cgctacattc cgaattaagt aaggcgtgat tatagcgcaa aaaatacggc gtgcctatac
     5941 cgttttgcct ttttgccgcg tgtcgggcgg atttaaagga gtgtgtttga atacagtgtt
     6001 gataatcata attatcttta agtaattcaa taagataact ttctacctga ccgaaaaaat
     6061 cattgccttt ccctgacaaa cggttgatga aatcggcaga ttgttgaaac gcagccggtt
     6121 taaaaggctt cgccgacttt cacgcctccc gccgtgtcct gcggcgaggc aagggcggca
     6181 acaaaggctt gcgccgcttg gaaatccgcc gtctgcatca cggcttgcgc ggcggcactg
     6241 ccgagcgtgt tggccatgta ttgccaacgt tgcgccaaag cgggattgtc gggaatgcgg
     6301 aaatcatcgc gcagttcgtc cacaaggtcg ggacggttgc agacgaggac aatgtcgcaa
     6361 cctgcctcaa aggaaatgcg ggcgcgttct ttgatgccgc ctgccccgca cgcgccctcc
     6421 atagtcaaat cgtccgagaa aatcacgcct ttgaacccga tgtcgcggcg caaaatctgt
     6481 ttcagccaga tttcggaaaa cccggcgggc tttgtgtcca cttgtggata aacgacgtgg
     6541 gcgggcataa ccgccgccat accttcgcgg ctcataatgc ggaaaggaat gaggtcggcg
     6601 gcttcgagtt cgtcgaggct gcgcccgtct tcgggtagca ccaaatggct gtccccttcg
     6661 acaaacccgt gtccgggaaa atgtttgccg caggatttca taccgccttt tgccaaacct
     6721 ttttgaaggg cgagggcgag gcgggttacg gcttcggggt tactgtggaa actgcggttg
     6781 ccgatgacgg ggcagtttcc ccagtccaaa tctaagacgg gcgtgaagga caagtcgatg
     6841 ccgcaggcgg aaagctcggt tgccaaaacc cggccgactt gtccggcggc ggtttcggcg
     6901 gcggacgcgc cgtctttgtc ccaaatctcg ccgagcgtac tcattgcggg caggcgggtg
     6961 aagccttcga tgaaacgttg cacccttccg ccctcgtgat cgacggcgat gatgagttcg
     7021 ggtgtgcgga gggctttgat ttcggcggtg agtgttttga gctgttcgat gttttggaag
     7081 ttgcggcgga agaggatgat gccgcctacg gcgggatcga gaaggcgttg cttttcctct
     7141 tcggtcaggc ggaaggcggc aatgtctgcc atgacggggc cgcgcggaat atggggaaca
     7201 gtcatttcgg tttgctccaa aaagcttcag acggcatatg ccgtctgaac agggaaaggg
     7261 gtcaggcgtt ggcgcgtttt ttatctttca acagaaaaat cagcaccgcc aatacaatgc
     7321 ctgtcgtgcc aaagcccaac agcgcggatt ttgtcagacc caatgcgagg tagcccgatg
     7381 cggcggcggc ggcaaccgtc agggcataag gcagttgcga ggtaacgtgg tcgatgtggt
     7441 tgcagcgcgc gcctgtggac gacaggatgg tcgtgtcgga gatgggcgaa cagtggtcgc
     7501 cgcataccgc ccccgccatt actgcggaca tacacgggat aatcagtgag ggatcgactt
     7561 tgaccgccat ggcggcggca atcggcagca tgatgccgaa cgtcccccag cttgtgcctg
     7621 tggcaaacgc catcacgctg gcgagcagga aaaggatgac gggcaggaag ccgggatgga
     7681 tgttgcccgc aaccagcgtg gagaggtagt cgcccgtgtg catttcgccg acaaccgtac
     7741 tgatgagcca ggcaaggatt aaaatggcga ttgcgccgaa catggatttc gcaccctgcc
     7801 aaacggcttt gggatagtcg gcggttttaa tcgtgccgag cgtgcagagg acgacggcaa
     7861 gcacaccgca agtgccgccg aagaccaggg aagtgtttac gtcggtattt tcaaatgccc
     7921 ccaaaatgct gaaggtttcg cttgcctgcg cgccggtgta gatcatggcg gaaaccgttg
     7981 aggcgattaa ggccaaaacg ggaataatca gtgcgtaaac acgacctttg gtagcgtctg
     8041 aaacggtagt ttcatcgtgg gcttcgttca acgcggcttg ttcgaaacgt gccatcgagc
     8101 cgatgtcgaa ggaaaaccat gcgacgacga acaccataat cagggcaaac agtgcgtaat
     8161 agttcatcag gctcatggcg acaaacgtcc ccatcggcgt gtattcggtg attttgtagg
     8221 taacgagcag tccggcaagc gtggcgataa tcgacgcgcc ccagcttgaa acgggcatca
     8281 gcacgcacat aggcgcggca gtggagtcga ggatgtaggc gagtttggcg cgggaaactt
     8341 taaacttgtc ggtaacgggg cgggcaatcg caccgacggc gagactgtgg aaatagtcgt
     8401 cgataaaggt tacgaacacg aggcaggcgg tcagcatttt cgcgccgcgc cggtttttaa
     8461 tgtgccgttt tgcccagtcg gcaaacgcct gattgctgcc ggagtaggtc agcagggaag
     8521 taaaaatacc caaaagtatc aggaaaacca agatttttgg tttgcccagc gaccaatcgc
     8581 cgtctgacca agccaagccg acgaccatgt ctttcaggtg tgtcagaccg tcgacggggt
     8641 tgccgccgac caaaaaggca acgccgacca gaataccgat gcctaaagac agcagtacgc
     8701 ggcgggtaat gacggcaagt gccagtgcca aaaagggtgg cacaaccgag aaaaatgaat
     8761 gtgaatagtc gatcagctgc atggttatgg tggtgttaag cgtccggatg ggagcgtatc
     8821 tgtccgcctc cggtttgggt tttgttggca aaatgggcgg aaatattttt tgtcgtaaaa
     8881 aatatttgtt taaaatcaac caactgattt ttgtaaaatg cccgttaatc ggtattgacg
     8941 ggcattttat catttaaaaa atattttggt taaattatgt gtgttattgc aggtttaatg
     9001 cgataaacag cgtgttgcca cggcgcatga ccagcagggg gacgtttttg cctgccttgt
     9061 ccatagcttt gcggaaaccg gcttcgtcat tgacggggac ttgcccgacg gcaagaattt
     9121 cgtcgccgcg cctcaagcct gcgcgttctg ccgcgtcgga aacccgtacg acgacgaggt
     9181 gtccgccgct gctgtcggta tgtgtctgaa gggtaatgcc tgcggattcg accgagaacg
     9241 taccggattg ctgttcggtg tagggggctt catctgtttt ggatgatgcg ccgatatgct
     9301 cggcggcgtt gcccagcttg actttgattg tgatttcttc gcctttgcgc catacgccga
     9361 ggctgacttc ttttcccggc gtaatggcgc cgaccataac gggaaggtcg ccggaagaac
     9421 gtatttctcc gccgtcgagg ctgaggacga tgtcgcccgc ctgcaggccg gcacgttctg
     9481 cggggctgcc gggcaggatt ttggcaatca gtgcgccgcc ggctttgtcc aaaccgaacg
     9541 attgtgccaa accgtaggat acttcttgaa taatcacgcc cagttgcccg cgttggactt
     9601 tgccggtgtt tttcagctgt tcggcgacat tcatggcaac gtcaatcggg atggcgaagg
     9661 aaatgcccat gaatccgccg ctgcggctgt atatttgcga gttgatgccg acgacctgtc
     9721 cttttaagtt gaacagcggg ccgccggagt tgcccggatt gatggcaacg tcggtttgga
     9781 tgaagggtgt gtagctttcg ttgggcaggc ttctgccttt ggcggacacg atgccggcgg
     9841 tcacgctgtt gtcgaagccg aagggcgcgc cgatggcggc gacccattcg cccggtttca
     9901 aatctttggg attgccgatt ttgacgacgg gcagctcttc cgttgcgtcg attttcagaa
     9961 gggcgacatc ggattggaca tccgaaccga tgagtttggc ggtatattcg cgcttgtcgt
    10021 tgagcaggac tttgatactg cccatgccgg taacgacgtg cgtattggtc agaatatagc
    10081 cgtctttgct gatgatgaag cccgaaccga agttcaatcc gccgtcatct gcttcttctt
    10141 gggggatttc gggcatattc gggacgaggc gtttgaaaaa ttcgtagaac gggtcgctgt
    10201 cggcaagcgg gtcggaatcg gtttcggcat tgccgctgcc gttttgggtg cgcggggcgg
    10261 gggctgcctg aatattgacg actgccgggc cttcgctttg aaccagttgg acaaagtcgg
    10321 gcagcagcat actgacgctg ccgtcgtctt tggtgtgttc gatgcgttct acgaaagatg
    10381 cttctttttt gtccgcaccg aaaaagctgc cggctttgtc gcagcctgcc agcgaggcgg
    10441 cacacagtgc tgccaaagcg aggtattggt attttttgaa cacgttttgt cctttgtcgg
    10501 atgccggtac cggctttaat gccgtctgaa gcgcattttg tcggcttcag acggcatagg
    10561 ttgaaattct acaacgtccg tccgaatttt caagcgtttc attttgaagg gcggcggcgg
    10621 tcaggctttg gcgggatatt cgcacaaatc gttgatgatg caggtttggc attgcggttt
    10681 gagtgccttg caggtgtagc gtccgtgcaa aatcagccag tggtgcgcgt ccatcagaaa
    10741 ttctttagga atgaagcgca tcagtttgtc ttcgacttcg cgcacatctt tcccgggggc
    10801 gattttggtt cggttggaca cgcggaaaat atgcgtatcg accgccataa cggggtgtcc
    10861 gaacgctgtg ttcaatacca cgtttgccgt tttgcgcccc acgcccggca acgattccaa
    10921 agcctcgcgg tcttccggca cttcgccatt gtatttttcc agcaggatgc ggcaggtctg
    10981 catgatgtgc ttggacttgg ttttatacaa cccgatggtt ttcgtatatt ccatcacgcc
    11041 gtccaaaccc aaatccagca tcgcctgcgg cgtatcggca accggaaaca gcttcgccgt
    11101 cgctttgttt acgccgacgt cggttgcctg tgccgacagt aaaacggcga ttaaaagctc
    11161 gaaaggggag ttgaagttga gttctgtggt cggatggggg ttggcggcgc ggaagcgttc
    11221 gaagatttct tggcggatgt gtctgttcat ttttttatac ggtgggtttg tgtgttcggc
    11281 attataacgt atggttcagg cggcgtaata ttgcattccc cacagaatga aggcgtaacg
    11341 cgccgttttg ccgataacca gcatcagccc gcttgtccac ggattcaacc gcagccagcc
    11401 ggcggcaagc ggcagtgcgt cgccgacgac gggcagccag gcaaacggaa gcagccaaat
    11461 accgaaacgc cgcatcagat tcagtgtttt ttcagacggc atttttcggg agggcagcaa
    11521 acgccccatc caataggaaa ccatactgcc caatccgttg gcaaggccgg cgcacagcaa
    11581 cgcgccgtat gcgtgttcgg gaaagcggtg gacgaacagg gcaaaggcgg cttcggatgt
    11641 gccgggcagg agggtggcgg aagtgaaggc ggaaaaggcg agggcggcgt aggtgtagga
    11701 gggtatcatt gcaaacagtc tcaaacaggt aacaatcggc gacggattg
//
LOCUS       DAXHKX010000041        11740 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHKX010000041 DAXHKX010000000
VERSION     DAXHKX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.041"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(340..762)
                     /locus_tag="ACFMWZ_000930"
     CDS             complement(340..762)
                     /locus_tag="ACFMWZ_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC5934910.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(880..1551)
                     /locus_tag="ACFMWZ_000931"
     CDS             complement(880..1551)
                     /locus_tag="ACFMWZ_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC5934911.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1567..2193)
                     /gene="purN"
                     /locus_tag="ACFMWZ_000932"
     CDS             complement(1567..2193)
                     /gene="purN"
                     /locus_tag="ACFMWZ_000932"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC5934912.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERSLEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2300..3118)
                     /locus_tag="ACFMWZ_000933"
     CDS             complement(2300..3118)
                     /locus_tag="ACFMWZ_000933"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC5934913.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3377..3817)
                     /locus_tag="ACFMWZ_000934"
     CDS             complement(3377..3817)
                     /locus_tag="ACFMWZ_000934"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC5934914.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3880..5286)
                     /locus_tag="ACFMWZ_000935"
     CDS             complement(3880..5286)
                     /locus_tag="ACFMWZ_000935"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC5934915.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5456..6571
                     /gene="lptF"
                     /locus_tag="ACFMWZ_000936"
     CDS             5456..6571
                     /gene="lptF"
                     /locus_tag="ACFMWZ_000936"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC5934916.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6568..7638
                     /gene="lptG"
                     /locus_tag="ACFMWZ_000937"
     CDS             6568..7638
                     /gene="lptG"
                     /locus_tag="ACFMWZ_000937"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC5934917.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7948..10533
                     /gene="acnB"
                     /locus_tag="ACFMWZ_000938"
     CDS             7948..10533
                     /gene="acnB"
                     /locus_tag="ACFMWZ_000938"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC5934918.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10675..11670
                     /locus_tag="ACFMWZ_000939"
     CDS             10675..11670
                     /locus_tag="ACFMWZ_000939"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC5934919.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2706 a         3260 c         3034 g         2740 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggatgcccgc ctcccgtcct cctgcagaag cgggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagc ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttccccttc
      301 agacggcatc agccgtttcc ccataatgcc gcccgatgcc tatttatctg ccccggcaat
      361 ttcaaaactg tgggtaatct ttgccgcttt gcccaacata atcgaagccg aacagtattt
      421 ttcggcagac atctgaacgg cgcgctcaat ggccgattct ttcaaatcat gcccgaatac
      481 tttgaaatgg atgtggattt cggtaaacac gcgcggcgca tcgtccgccc gtttcgccgt
      541 aaccgtcgca cggcagtcag tcactttctg acgctgtttt tcggcaatca tcaccacatc
      601 gatgctcgaa cagcccgcca cgcccaacag cagcatttcc aaagggctgg gcccgcgctt
      661 ggccgcacct tctgccgccg aaccctccat aacgacgctg tgcccgcctt ccgttgttcc
      721 gacaaagcac attccgtcta tccattttga tgtaacctgc atggtgtcat tcctgaaaat
      781 agcgttaaaa ccgctttgca tatggcgtta ttgtaaacaa tttcaagcgg cttatgcaga
      841 aatatggaca aaacggcaaa aaaacgcctg aaaatcgatt tacggtttgg cggactggcc
      901 attgatctgc accgatttga gtttcagcgt ataggttttg ccgtcgtcgg tatagccgat
      961 ttgtgccgga atattgttca gggacggtgc gaagaaatac attaccgcat cgtcgccgcg
     1021 ccgcacccga tatttgacga cttcggtttc cacgccgcct atgctgtatt ttcctgtacc
     1081 cgccttattc aaaccgccga cggaataaag ttttttgccg ttggtgattt tcagccccgg
     1141 ggggagtttc gcgtcatttg ccgccaactg ccaggcaagc gtgaacaaat ccatagcctt
     1201 ggggctttgc tcggttttgc tctcgcccgc tttgccgtaa gttacgctgc cgtcggcgaa
     1261 tttggcttcc gcatacagtt tgcccctgcg tatgtctcta tagtaggtag ggtgcagggt
     1321 attgccgaca accgtaccgc cggactcgaa acggatattg tatagcggca ctttaatcgt
     1381 cgaaacgatt ttgtaagcat tgccgctgcg ttcaaatgtc atcgtggcgg gaatgccgta
     1441 gctgccggaa tagtgcagca cggcggattg gggcagccct gccgcatacg cgcacggcag
     1501 ggcggcggac aaaatggcgg cggaaaatat atttttaaaa gtcttcatca tttgctcccg
     1561 cccggtttac gccgtcagga aacgggcggc atcggcgttt tccgaatttc tgacgcggtt
     1621 tccctcgata atcaggcggc cggcggcaaa atcggcaacg gctttcggat agagtttgtg
     1681 ttcgacagcc aaaacccggg cggcaatatc gtctgccgta tcaccatcaa gtatcggcac
     1741 aatcccttgc gatacaatcg ggccgcaatc cagctcggcc gtaacaaagt gaatcgtaca
     1801 gcctgcaacg cggcaacccg cttccaaaga gcgttcgtgc gtatgaagcc cagtaaatga
     1861 aggcaggatg gacggatgga tgttcatcag cctgccttcg taatgggcgc aaaactcggg
     1921 ggtcagaatc cgcataaaac ctgccaaaac caccaagtcg ggttgatatg cgtcgatttt
     1981 ttccatcatg gccgtatcga aggcaagtcg tgattcaaag tttttatgat tcaggctatc
     2041 ggtcgggatg ccgcgttcgg ccgcccattg caaaccggca gccgtttcgc tgttgctcaa
     2101 cacggcggca atgcggacgt tgggaatggc ggcattgacg attgcctgca tattgctgcc
     2161 gcgtccggaa atcaggatga cgatgttttt cataatggta cgcttttgaa agggatgccg
     2221 tctgaaccgc tgtttggtgg tttcagacgg catttgccgt aaaaatgccc gaaaacctgt
     2281 ttcgggcatg gattcggact taatttactt ttttgatgtc gacttgagcc ggctgcttgg
     2341 cgggcgcgtt ttcgggtgcg ccgattttga ccagtttcac atcaaatacc aaagtggcgt
     2401 tcgggccgat tttgtcgccc gcaccctgtt cgcggtaggc aaggttggac gggatgtaga
     2461 acgtggcttc gccgccttct ttcagaagct gtacgccttc ggtccaaccc ggaatcactt
     2521 ggctcaaagg gaaggtgacc gggccgccgt tggctttgct gctgtcgaat accgtaccgt
     2581 caatcaggcg gccttcgtat tccacggtaa cgatgtcgtc tttggtcggc tgtttgcctt
     2641 cgccctgttt ggtgattttg tattgcaggc cggaagcagt ggtcttcacg ccgtctttgg
     2701 cggcattttc tttcagaaag gcttcgcctt tttctttatt ggccttcgcg tccgccttgt
     2761 gtttttctac ggctttagcc tgttgttcct gaaggaattt catcatgact tcctgagcct
     2821 gctcttcggt cattttgatt tctttgccgt catacactgc ctgcatggct tcggtaaaga
     2881 ctttcaaatc gatttccgcg ccctgttcct tcatttgctt cagggagcgt ccgatgtcca
     2941 cgcccatcgc atagcttgcc tgctgcatcg tgctgccgat cgaagaggtg tcgccctgcg
     3001 cggaagaagc ggcggcaggt tcggatgcag atgcgggggc ggcttctttt ttgccgcagg
     3061 cggaaagtgc caaagcggcg gaaagggtca gtgcgctgat tttgaaaatg gtgttcatga
     3121 tggatcttcg ctgtcgataa ggtcggaaaa acgggattat agccgagttt gaatgtttca
     3181 acacacagga tgacacataa agcgtcaatc gtgtgttgcc ctgttttgga aaggattgaa
     3241 ccttccaaaa taagttttga ttctaccgcc ccgagggaca gatgtccaag tggcggggtt
     3301 caaccgataa ggaaatttta atcaaataga atcaagcctg tttaaatttt gtaaatgcgg
     3361 catttcagac ggcattttat gccttgccct ccatgccgtg atgttcgatg gcaaaaccgc
     3421 ttcggcggta ggcggtaaag cgttcgcgcg cgtcggcgag atcttccaag ctgttgccga
     3481 cgatttccaa aacgcgcgcg ggcaagaccg gagcggtgtt ccaaaaaccg tcggacaggt
     3541 tcaaaacggt catgccttcg ggaattcggg gcagattgcc gccgcaggca agcaggacgg
     3601 atggttcaga cggcatggct tcttccgttt cccaaatttc gtgcggaatg aaactctcgg
     3661 cctcgtattg ccaaagcatt ttgtccaatt cctgaagctg cccgaacgaa tcggaccaca
     3721 ccagtatcct gccgccgtcc cgaatcgcac gggcaatcag gcggcaggtg aaaatcggaa
     3781 cttgggcaac gtgcgtgtaa aaggtggctt tcggcatatt gtttgaacat ttggcaggat
     3841 aatgccgtct gaaaggcttc agacggcatt gtggcaaaat taaagattcc gcagatagtt
     3901 cagcagcaag ggaacgggac ggccggtcgc acctttttcc gcaccagatt tccacgccgt
     3961 acccgcgatg tcaaggtgtg cccatggata gtcttcggta aagtaggata ggaatgttgc
     4021 ggcggtaatc gtgcccgcgc cgggcgtgcc gatgtttgga atgtcggcaa agttggattt
     4081 gagttggtct ttgtaggtct caaagagcgg cagttgccat gctttatcgt ccacgttgta
     4141 ggaagcggca agcaggctgt cgatcaaatc ctgattgttg cccatcacgc cgctgacatc
     4201 gtgccccaag gcaacaatac acgcgccggt cagggtggcg acgtcgatga cggctttggg
     4261 tttgaactgc tcggcgtaag tgagcgcgtc gcacaaaatc agacggcctt cggcatcggt
     4321 gttcaacact tcgatggtca gacctttcat acttttcacg acatcgcccg gtttgtttgc
     4381 cgcgccggaa ggcatatttt cacaagtggc gacgacggca atcaggttaa tcggcagttg
     4441 cagtttgacg gcggcgcaga aggtgctgat gacggttgcc gcgccgcaca tatcaaactt
     4501 catttcgtcc atgttcaggc cgggtttgag ggaaatgccg ccggtgtcga aggtaatgcc
     4561 tttgccgacc aataccacag gcgcggcttc tttgtccgcc gcaccgaaat agctcagttc
     4621 gaccaaatag ggatcttcga cactgccttt ggcgaccgac caaaacgaac ccatgttttc
     4681 tttgatgtag tctttttcaa tgattttggc gtgtgcgccc aatttttcgg cttcggcctt
     4741 ggcggtgcgc gctaaaaatt cgggcgtgca ttcgttcggc gcggcattgc ccaagtcgcg
     4801 gcagaggctt tgtccgtaaa cttgcgcttc ggcgacgcgc aaggcttctt taacggcggc
     4861 ttcgtgtgcg ctgtggaaca cggcagtttc aaatttggcg ggcttggctt cttttttgta
     4921 gcggtcgaaa cggtaggcgg cgttgccgaa cgcaatcgca aacgcttcgg ctacggcggc
     4981 ggcttgcgct tcatcgaagg catggatgtc cacattgacc gtttcctgat tttgcgccca
     5041 tttggcggct tcggcggcgg ccttgttcaa tgcggcgcgg ccggtgcttt tcagacagca
     5101 tacggcaaca gcctgcaaac cgttgcctgt cgggattttt gtgtcggcaa aattttgacc
     5161 ttcttcaagc gaagacaaaa gggcaagggc ggtcggatgg tttaattgcg cctcttcggt
     5221 gcagacaaat aactgtgcgc ctgcctgctg ttcctgcaag atttcggttt ttgtgctaaa
     5281 ttccacgttt attctcctga ttgagacggt tgtcggtagt tttcggacgg cctttcgctc
     5341 aaaagaccgt ctgaagacgg ctggcacgat tgtaccccat ttgaagcacc gtctgaaacc
     5401 ttgcgcggac aatccgcctg cgccgaaccg cttaccgccc cctgaccgcg attctatgat
     5461 ttatcaaaga aacctcatca aagaactctc ttttaccgcc gtcggcattt tcgtcgtcct
     5521 cttggcggta ttggtctcca cgcaggcaat caacctgctc ggccgtgccg ccgacgggcg
     5581 tgtcgccatc gatgctgtgt tggcattggt cggcttctgg gtcatcggta tgacgccgct
     5641 tttgctggtg ttgaccgcat ttatcagtac gttgaccgtg ttgacccgct actggcgcga
     5701 cagcgaaatg tcggtctggc tatcctgcgg attggcattg aaacaatgga tacgcccggt
     5761 gatgcagttt gccgtgccgt ttgccgtttt ggttgccgtc atgcagcttt gggtgatacc
     5821 gtgggcagag ctacgcagcc gcgaatacgc tgaaatcctg aagcagaagc aggaattgtc
     5881 tttggtggag gcaggcgggt tcaacagttt gggcaagcgc aacggcaggg tttattttgt
     5941 cgaaaccttc gataccgaat ccggcatcat gaaaaacctg ttcctgcgcg aacaggacaa
     6001 aaacggcggc gacaacatca tcttcgccaa agaaggtaac ttctcgctga acgacaacaa
     6061 acgcacgctc gaattgcgcc acggctaccg ttacagcggc acgcccggac gcgccgacta
     6121 caatcaggtt tccttccaaa aactcaacct gattatcagc accacgccaa aactcatcga
     6181 ccccgtttcc caccgccgta ccattccgac cgcccaactg attggcagca gcaacccgca
     6241 acatcaggcg gaattgatgt ggcgcatctc gctgaccgtc agcgtcctcc tactctgcct
     6301 gcttgccgtg ccgctttcct atttcaaccc gcgcagcgga catacctaca atatcttgat
     6361 tgccatcggt ttgtttttaa tttaccaaaa cgggctgacc ctgctttttg aagccgtgga
     6421 agacggcaaa atccattttt ggctcggact gctgcctatg cacatcatca tgttcgtcat
     6481 cgcaatcgta cttctgcgcg tccgcagcat gcccagccag cccttctggc aggcggttgg
     6541 caaaagtctg acattgaaag gcggaaaatg aacctgattt cacgttacat catccgtcaa
     6601 atggcggtta tggcggttta cgcgctcctt gccttcctcg ctttgtacag cttttttgaa
     6661 atcctgtacg aaaccggcaa cctcggcaaa ggcagttacg gcatatggga aatgctgggc
     6721 tacaccgccc tcaaaatgcc cgcccgcgcc tacgaactga tgcccctcgc cgtccttatc
     6781 ggcggactgg tctctctcag ccagcttgcc gccggcagcg aactgaccgt catcaaagcc
     6841 agcggcatga gcaccaaaaa gctgctgttg attctgtcgc agttcggttt tatttttgct
     6901 attgccaccg tcgcgctcgg cgaatgggtt gcgcccacac tgagccaaaa agccgaaaac
     6961 atcaaagccg cggccatcaa cggcaaaatc agtaccggca ataccggcct ttggctgaaa
     7021 gaaaaaaaca gcattatcaa tgtgcgcgaa atgttgcccg accataccct gctgggcatt
     7081 aaaatctggg cccgcaacga taaaaacgaa ctggcagagg cagtggaagc cgattccgcc
     7141 gttttaaaca gcgacggcag ttggcagttg aaaaacatcc gccgcagcac gcttggcgaa
     7201 gacaaagtcg aggtctctat tgcggctgaa gaaaactggc cgatttccgt caaacgcaac
     7261 ctgatggacg tattgctcgt caaacccgac caaatgtccg tcggcgaact gaccacctac
     7321 atccgccacc tccaaaacaa cagccaaaac acccgaatct acgccatcgc atggtggcgc
     7381 aaattggttt accccgccgc agcctgggtg atggcgctcg tcgcctttgc ctttaccccg
     7441 caaaccaccc gccacggcaa tatgggctta aaactcttcg gcggcatctg tctcggattg
     7501 ctgttccacc ttgccggacg gctcttcggg tttaccagcc aactctacgg catcccgccc
     7561 ttcctcgccg gcgcactacc taccatagcc ttcgccttgc tcgccgtttg gctgatacgc
     7621 aaacaggaaa aacgctaaac caatgccgtc tgaccccctt cggacggcat tggtttttat
     7681 tatcatattc ctgtagacag atagccgttc cctattatag tggattaaca aaaaccagta
     7741 cggcgttgcc tcaccttgcc gtactattgt actgtctgcg gcttcgtcac cttgtcctga
     7801 tttttgttaa tccactatac attacctgtc ataacagttc catttttgtt aaaactagtc
     7861 tatgatagcg gtacaaatat tgtttacaat atttaacgca aatcatttgc aacccgacaa
     7921 aagaaaaaca gaaaaaggaa caaagagatg ttagaagcct atcgtaaagc cgccgccgag
     7981 cgcgccgccc tcggcattcc cgccctccct ttgaacgcgc agcaaaccgc cgatttggtt
     8041 gagctgctga aaagcccgcc cgcaggcgaa ggcgagttct tggtcgaact gcttgcccac
     8101 cgtgttccgc ccggtgtgga cgatgccgcc aaagtcaaag cctcattcct ggctgccgtt
     8161 gccgaaggca gcgcgtccag cccgctgatc tcccccgaat atgcgaccga actcttaggt
     8221 acgatgctcg gcggttacaa tatccacgcc ttaatcgaac tcttggacga cgacaaactc
     8281 gcgcccattg ccgccaaagg cttgaaacat acgcttctga tgttcgattc cttccacgac
     8341 gttcaagaaa aagccgaaaa aggcaacaaa tacgcgcaag aagttttgca atcttgggca
     8401 gatgccgaat ggttcgcctc acgcgccaaa gttcccgaaa aaattaccgt taccgttttc
     8461 aaagttgacg gcgaaaccaa tacagacgac ctctcccccg cgcccgacgc gtggagtcgt
     8521 cccgatattc cgctgcacgc gctggccatg ctgaaaaacc cgcgcgacgg catcacgccc
     8581 gacaaaccgg gcgaagtcgg tccgattaaa ttgttggaag aactcaaagc caaaggccat
     8641 ccggttgctt acgtcggcga cgtggtcggt actggttctt cacgcaaatc cgcgaccaac
     8701 tccgtcattt ggcataccgg cgaagacatt ccgttcgtgc cgaacaaacg cttcggcggc
     8761 gtatgtttgg gcggcaaaat cgcgccgatt ttcttcaata cccaagaaga ttccggcgcg
     8821 ctgccgattg aagtcgatgt atctgctcta aaaatgggtg atgtcgtcga tatcctgcct
     8881 tatgaaggca aaatcgtgaa aaacggcgag actgttgccg agtttgaatt gaaatcacaa
     8941 gtattgctgg acgaagtgca agccggcggc cgtatcaacc tgattatcgg ccgaggtctg
     9001 accgccaaag cgcgcgaagc cctgaaactg cctgcctcta ctgcattccg cctgccgcaa
     9061 gcgcctgccg aaagcaaagc cggtttcacc ttggcgcaaa aaatggtcgg ccgcgcctgc
     9121 ggtctgcccg aaggacaagg cgtgcgcccg ggtacttact gcgaaccgcg tatgacgacg
     9181 gtcggctcgc aagacacgac cggcccgatg acccgcgacg agttgaaaga cttggcttgt
     9241 ttgggcttct ccgccgatat ggtgatgcag tctttctgcc acaccgccgc ctatccgaaa
     9301 cctgtcgatg taaaaaccca taaagaactg cccgccttta tttccacccg tggcggcgtg
     9361 tcactgcgtc cgggcgacgg cgtcatccac tcgtggctca accgcctgct gctgcccgat
     9421 accgtcggca ccggcggcga cagccatacc cgtttcccca tcggtatttc cttccccgcc
     9481 ggctccggct tggttgcctt tgccgccgca acgggcgtaa tgccgctcga tatgcccgag
     9541 tctgtattgg tacgcttcag cggcaagctg caaccgggcg taaccctgcg cgatttggtg
     9601 aacgccatcc cgctgtacgc aattaaacaa ggtttgctga ccgttgccaa agccggtaag
     9661 aaaaacatct tctctggccg catcctcgaa atcgaaggcc tgcccgattt gaaagtggaa
     9721 caagcctttg aattgaccga cgcatccgcc gaacgctccg ccgccggctg taccgtgaag
     9781 ctcaataaag agccgattat cgagtacatg aaatccaacg tcgtgttgat gaaaaacatg
     9841 attgccaacg gctatcaaga cccgcgcact ttggaacgcc gcatcaaagc catggaaaaa
     9901 tggctggcaa atcccgagtt gctcgaagcg gataaagatg ccgaatacgc cgccgtgatt
     9961 gaaatcaaca tggacgacat caaagagccg attatcgcct gcccgaacga cccggacgac
    10021 gtgtgcttca tgtccgaacg ctccggcacc aaaatcgacg aagtattcat cggttcgtgt
    10081 atgaccaaca tcggccactt ccgcgccgcc tccaaacttt tggaaggcaa ggcagacacc
    10141 cccgtccgcc tgtggattgc gccgccgacc aaaatggacg cgaaacaatt gtccgacgaa
    10201 ggacactacg gcgtactcgg acgtgccggc gcgcgtatgg aaatgccggg ttgctcctta
    10261 tgtatgggta atcaggcgca agtacgcgaa ggtgcgaccg ttatgtccac ctccacccgc
    10321 aacttcccga accgtttggg taaaaacacc tttgtttacc tcggttcggc ggaattggca
    10381 gcgatttgct ccaaactggg taaaatcccg accgttgaag aatatcaagc caatatcggc
    10441 atcatcaacg aacagggcga taaaatctac cgctatatga acttcaacga aatcgacagc
    10501 tacaacgaag tagccgagac cgtgaacgtt taatccccgt catccgtatg aagtaaggga
    10561 ttgaccgcaa tgccgtctga acaaccttca gacggcattg caacattccg ctaacccttc
    10621 tttccgcaaa cgctgcaaat acggcgttca cgcccccaca taaaggaaac gacagtgaac
    10681 ctgaaaaacc gccattttct gaaactttta gacttcacgc cggaagaaat caccgcctac
    10741 ctcgaccttg ccgccgaatt gaaagccgcc aaaaaagcag ggcgcgagat tcagcggatg
    10801 aaagggaaaa acatcgccct gatttttgaa aaaacctcta ctcggacgcg ctgcgcgttt
    10861 gaagtcgccg cgcgcgatca aggcgcggga gtgacttatt tagagccgtc cgccagccaa
    10921 atcgggcata aggaaagcat caaagacacc gcccgcgtgt tgggcaggat gtacgatgcc
    10981 atcgaatatc gcggtttcgg tcaggaagtt gttgaagaat tggcgaaata cgcgggcgta
    11041 cccgtgttca acgggctgac caacgagttc catcccacac aaatgcttgc cgacgcactg
    11101 actatgcgcg aacacagcgg caaacctttg aaccaaaccg cgtttgccta cgtcggcgac
    11161 gcgcgttaca acatgggcaa ttccctgctg attttagggg caaaattggg gatggacgtg
    11221 cgtatcggcg caccgcaaag cctgtggccg tctgaaggca ttattgccgc cgcacacgcc
    11281 gccgccaaag aaaccggcgc aaaaattacc ctgaccgaaa acgcgcatga agccgtgaag
    11341 aatgttgatt ttattcatac cgatgtgtgg gtcagcatgg gcgagccgaa agaagtctgg
    11401 caggaacgca tcgatttgct gaaagattac cgcgttacgc ccgaactgat ggcggcatcg
    11461 ggcaatccgc aagtcaaatt catgcactgc ctgcccgcct tccacaaccg cgaaaccaaa
    11521 gtcggcgaat ggatttacga aaccttcggg ctgaacggtg tggaagttac agaagaagta
    11581 ttcgaaagcc ccgccagcat cgtgttcgat caggcggaaa accgtatgca cacgattaaa
    11641 gcggtaatgg tcgcggcttt gggcgactga cagaactgtg cctgtttaaa ttcatccgca
    11701 acacagatac cgtctgaaca cgatgttcag acggtatcca
//
LOCUS       DAXHKX010000042        11612 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHKX010000042 DAXHKX010000000
VERSION     DAXHKX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.042"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(176..868)
                     /locus_tag="ACFMWZ_000940"
     CDS             complement(176..868)
                     /locus_tag="ACFMWZ_000940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC5934920.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(976..2169)
                     /locus_tag="ACFMWZ_000941"
     CDS             complement(976..2169)
                     /locus_tag="ACFMWZ_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC5934921.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2298..3035)
                     /gene="pxpA"
                     /locus_tag="ACFMWZ_000942"
     CDS             complement(2298..3035)
                     /gene="pxpA"
                     /locus_tag="ACFMWZ_000942"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC5934922.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3022..3951)
                     /locus_tag="ACFMWZ_000943"
     CDS             complement(3022..3951)
                     /locus_tag="ACFMWZ_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC5934923.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(3948..4595)
                     /gene="pxpB"
                     /locus_tag="ACFMWZ_000944"
     CDS             complement(3948..4595)
                     /gene="pxpB"
                     /locus_tag="ACFMWZ_000944"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC5934924.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4779..6986
                     /gene="uvrD"
                     /locus_tag="ACFMWZ_000945"
     CDS             4779..6986
                     /gene="uvrD"
                     /locus_tag="ACFMWZ_000945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC5934925.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVNVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKIAAFVRLIEALRNQVGQLPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNATLESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSIKKTAYDSYGNTRQTATFKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7343..7882
                     /locus_tag="ACFMWZ_000946"
                     /pseudo
     CDS             7343..7882
                     /locus_tag="ACFMWZ_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
     gene            complement(8072..8287)
                     /locus_tag="ACFMWZ_000947"
     CDS             complement(8072..8287)
                     /locus_tag="ACFMWZ_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934926.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8411..8770)
                     /locus_tag="ACFMWZ_000948"
     CDS             complement(8411..8770)
                     /locus_tag="ACFMWZ_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC5934927.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8823..9200
                     /locus_tag="ACFMWZ_000949"
     CDS             8823..9200
                     /locus_tag="ACFMWZ_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC5934928.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            9399..10019
                     /locus_tag="ACFMWZ_000950"
     CDS             9399..10019
                     /locus_tag="ACFMWZ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC5934929.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            10042..11493
                     /locus_tag="ACFMWZ_000951"
     CDS             10042..11493
                     /locus_tag="ACFMWZ_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC5934930.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         2859 a         3079 c         2833 g         2841 t
ORIGIN      
        1 acctctttca gttacagcag cttgatccct ttcccttatc caacggggga aggctaggat
       61 agggtggctt gcaaatatac agaacaaggg acaagagcca ccctctctcc aaccctctcc
      121 ctccgtacgg gagggggtgg attctcgcgg gcgaagccca cgctacggtt agcctttacc
      181 ccagcacaaa caattcccgc ccgtgcgcct tcagccaact tttagcattg tcggtatgcg
      241 gcgtcagcgt gttcaccaaa tgccaaaagc gcggactgtg gtcggggtgg cggaggtggc
      301 agagttcgtg gatgcagaca tagtcggcga cgtattcggg cgtgccgatc agccgccagt
      361 tgaggcggat gccggtgtgc gggcggcata cgccccaaaa ggttttggcg ttgctcaggt
      421 ctgtggcggt gggcgtcagt cctgtttcgg ctgcgtgttt ttcaaggcgg ggcagcaggt
      481 attcgcgggc gcgttcgttc aacaggcggc gcaggtggtc gatttgtgcg gcggtttctt
      541 ttcggggaag caggatttca gacgacgtga tacggatatg gctttggctg tgggtatcca
      601 gcttggtctt tattccccga taccaaatcc actcgggtaa gtttgggtgg gaaacaggat
      661 gcacgggcgt tttggcaagc gtgttccgca aaatcgtttc gtttgccgcc agccagtttg
      721 ctaacgcgtg gtcttgaaaa aagggtggga cgttgatgct gaccgtctgc atattgacgg
      781 ggcgcagaat cagatttttc ttggcactgc gtttgagttc gatttcgatg cacaagccgt
      841 cggaaagagt ataggtgaag cgtttcatag ttgtgaatag gtttcagacc ggatacatcg
      901 tctgaaacag gaattttcca tatcaggcgg caaacttcgg ataatataca aaatcaaaca
      961 tctgcgctac aaggttcagc cgaacaagcc gccgatatat ttgctgatgg taatggcgct
     1021 gagtactgcc atcaaaccga ccacaatcac gccggaaacg gtgagccaca gcgggtgttt
     1081 gtagtcgccg acaattttgg ttttgtaggc ggcaatcaga atcagaccga gggaaatcgg
     1141 taaaatcagg ccgtttaatg cgcctacgaa caccagcacc tgcgccggtt tgccgatggt
     1201 ggaaaatacg gcggtggaca cggcgataaa ggcaataatc catttgtttt tattgcgttc
     1261 gatagacggg ctgagaccgg agaagaacga caccgaagta taagccgcac caatcaccga
     1321 agtaatcgaa gccgcccaaa tcaccacgcc gaaaatcagc aggccgatgt atcccgccgc
     1381 atattcaaac ggtgtggaag cagggttgtc gggattgagc tgtacgcctt ggctgaccac
     1441 gcccaaaacc gccaaaaaca atacaatccg cataatcgag gcaatcagga tcgcccgcac
     1501 cgagctttgg ctcacttccg gcaacgccga tttgcctttg atgcctgcgt ccagcagacg
     1561 gtgcgcaccg gcgaaggtga tgtagccgcc gaccgtgccg cccaccagtg taacaatcgc
     1621 cattgcatcg agtttttccg gcataaaggt atgcacggcg gcatctgcca gcggcggatt
     1681 cgcctgccat gccacataaa ccgtcagcgc aatcattacg aaacccatca cttgggcgaa
     1741 tttgtccatc actttgcctg cttctttaaa cagaaacaca ccgatggcaa tcacgccgct
     1801 gatcacggca ccggtttccg gcgacagtcc ggtcagcagg ttcagaccca agcctgcgcc
     1861 gccgacgttg ccaatattga acgccaaacc gcccatcaca atcagcacag ccaagaaata
     1921 gcctgcgccg ggcaagacct gattggcaat atcctgcgcc tgtttttcgg aaacggcgac
     1981 aatccgccaa atattgagct gcgccccgat gtcgagcaga atcgagagca gaatcacaaa
     2041 gccgaaactt gccgccagtg cttgggtgaa ggtggcggtt tgggtcagaa agcccgggcc
     2101 gatggcggaa gtcgccatca ggaatgcagc gccgattaag gcatttctgc ggtttttttg
     2161 attagacata atcgcttatc ctctataaaa ttggttgttg ctgtgtttgg gcgaaacctg
     2221 cggttttagc tacgcagaaa ctcgctttgc tcgttttggc gaaacctgcg gttttcagac
     2281 ggcctatgaa ctgtttttca agcagaaact ttgatgcctg ccgccagtag ttcctgccgg
     2341 attttttcgg caaacaccac ggcgtgcggc ccgtctccgt gcagacagat gctgtcggct
     2401 tgcacggcaa ccaggctgcc gttcactgct ttgacccgcc cgtcccgcac catctgcaat
     2461 acttgggcga tggcttcttc gtcgctgtcc acctgcgcat cggggcggct gcggggaacc
     2521 agcgtaccgt cgggcatata gcggcggtcg gcgaatactt cggaaatcac acccaagcct
     2581 gcggcttttc cggcttccaa gagcaggctg ccggaaagtg ccatcaattt caatttcggg
     2641 tcgaaatccg ccacaattcg ggcaacggta tccgccagcg cacggttttt cgccgcttgg
     2701 ttgtacattg cgccgtgcgg tttgacataa gccatttcca aaccctgatc acggcacaag
     2761 gcctgcaatg cgcccaactg gtaattcaga cacgcccgca aatcggcttc ggacagattc
     2821 atttcggtac ggccgaagtt ttcccgatcg ggatagccgg ggtgtgctcc gatgcgcacg
     2881 ccgttttgtt gggcatacgc caatgccgcc cgaatatcgg caatgctgcc ggcgtgttgg
     2941 gcgcaggcga tgttggccga agtaatcagc tgcaacaagg cttcgtcgct gccgcagcct
     3001 tcggcgagat cggcgtttaa atcaacctgc ttcatgggtg attctccgta tttggttcag
     3061 atagacttgg tttttgcgcc gcagggcggt ggcttctttc aagccgatta ttttgaattt
     3121 gactttgctg ccgaagcgca cctgtgccag cctgcccaaa tcggcggcgg caacggtagc
     3181 gattttcgga taaccgccgg tggtttgcgc atcggccagc aggataatcg gtttgccgcc
     3241 gggcggcacc tgcacggttc ctgcctgaac agcgtgggac agcatttcca aaggttgcga
     3301 cagggtcagc ggctgtccgt cgaagcggta gcccatgcgg ttgctatcgc tttgcagcgt
     3361 ccacgtttcc cgttccagat tcagacgccc tttttcactg aaagcggcat attccgacga
     3421 aggaacaagg tggacggtat cggtaaacgg tatcggggca atgccgactt tggacaattc
     3481 ctgcgcacct ttgccgatgg ggagacaatc acctttttgc agcattctgc cctgatggcc
     3541 gccgaaaccg gctttcaggt cggtgcttct cgaacccatc acttccggca catcaaatcc
     3601 gcccgccacg cacacatagc cgtacatgcc ctgcacggca cgcaccattt tcaaggtctg
     3661 ccctttgcgg gcggtataac gccaatacga atagaccggt tcgccgtcca attccgcctg
     3721 atacacggca ccggtgagac aaaacggcgt atcccgttca aacaccagca ttatcccgcc
     3781 caaagcgatt tcgattgcgg ccgtgccttc gtcgttgccc aataaaatat tacccgccgc
     3841 caaagcaacc gtgtccatcg caccggcatg accgatgccg taacgccggt gtccgtagcg
     3901 tccggtatcc tgaatatgcg ccggtgcctg cactgccgaa acgtgaatca tggctcaatc
     3961 ctttctgcaa caaagcggac ttggtcaccc gccgccagca gggtcggcgg attcaaatcg
     4021 gctcggaaca agggtaattc ggttctgccg ataatctgcc agccgccggg cgaagcgaac
     4081 ggatacacac cggtctgact gccgccgata ccgaccgaac cggcaggaac ggacgttctc
     4141 ggcacggcac ggcggggcgt gtgcaatgct tcgggcaagc cgcccagata agggaaaccg
     4201 ggctggaagc ccatcataaa tacggtataa gtttgcgccg tatggcggcg gacgatttcg
     4261 gaaataaccg tctgatggaa agcagcgact tccgccaaat ccgggccgta ttcgccgccg
     4321 tagcagacgg gaatttccac cagtttgccc tgatggtctg taacggcggt gtgttcccac
     4381 acatattgca attcatcggc aagcgtcgcc aaatcggtat cgaaacgggt gaacacggtc
     4441 agattgttca tgccgaccac cacttcctca atcctgtcgt gctgcccgag cgcagcggca
     4501 aacgcccaca acttttgctg tttgcccagt tcggaaggcg cattcagtcg gcagaccaaa
     4561 gcggattcgc tgattggtgt gatctctatt ctcatttgtt gttcattttg gttatgtttt
     4621 aatgaatcta tatgcagggg cggcggtttg tcaatatctt ctgtgctgca tcatcaaacc
     4681 gtcgattgga aaagtgctgc cctgccgctg cactttttca gacgacctta aaccgtttct
     4741 attaaaatag cgcattccac ttttcagacg gcatccctat gtttcccgac caatccgccc
     4801 ccaacctcct gcaaggcttg aatcccgaac aactctccgc cgtaacctgg ccgccgcaat
     4861 ccgcccttgt gctggcgggc gcgggcagcg gcaaaacgcg cgtgctgacc acgcgcatcg
     4921 catggctttt gcaaagcgga caagccagcg tgcatagcat tatggcggta acgtttacca
     4981 acaaagccgc caaagaaatg cagacccgtt tgggcgcaat gattcccgtc aacgtccgcg
     5041 ccatgtggct cggcacgttt cacggtctct gccaccgctt tttgcgcctg caccaccgcg
     5101 acgcaggcct gccgtcttcc tttcaaatcc tcgacagcgg cgaccagctt tccctcatca
     5161 aacgcctgct caaaagcctc aacatcgccg aagaaatcat cgcgccgcgt tcgctgcaag
     5221 gctttatcaa cgcgcaaaaa gaatccggct tgcgcgcttc cgtcttgagc gcgcccgacc
     5281 cgcacacaag ccgcatgatc gaatgctacg ccgaatacga caaaatctgc caacgcgaag
     5341 gcgtggtcga ttttgccgaa ctcatgctcc gcagctacga aatgctgcaa agcaacgaaa
     5401 tcctgcgcca acactaccaa aaccgcttca atcacattct ggtcgacgaa ttccaagaca
     5461 ccaacaaact gcaatacgcc tggctcaaac tcatggcggg cggcaacgcg gcggtatttg
     5521 ccgtcggcga cgacgaccaa agcatctacc gtttccgtgg cgcaaacgtt ggcaatatga
     5581 ccgcgctgat ggaagaattc cacatcgacg cgcccgtcaa actcgaacaa aactaccgct
     5641 ccgtcggcaa catcctcgcc gccgccaacg ccgttatcga aaacaacgac gaacgcctcg
     5701 gcaaaaacct gcgcaccgac gccgaagcag gcgacaaaat ccgctactac tccgccttta
     5761 ccgacctcga agaagcccgg ttcatcgtgg acgaaaccaa agccctcgaa cgcgaaggct
     5821 gggatttgga cgaaatcgcc gtcctctacc gcagcaacgc ccaatcgcgc gtcatcgaac
     5881 aaagcctgtt ccgcagcggc atcccctaca aaatctacgg cggcttgcgc ttttacgaac
     5941 gccaagaaat caaacacgcg ctcgcctacc tacgcctcgc cgtcaatccc gacgacgaca
     6001 acgccctttt gcgcgtcatc aacttcccac cgcgcggcat cggcgcacgc accgtcgaga
     6061 accttcagac ggcctcaaac gaacaaggca tcaccctttg gcaggcagcc tgcaacgccg
     6121 gcgcgaaagc cgccaaaatc gccgccttcg tccgcctgat tgaagcactg cgcaaccaag
     6181 tcggacaact gcccctgtcc gaaatcatcg tcggcatcct caaagacagc ggcctgaccg
     6241 aacactaccg aacccaaaaa ggcgacaacc aagaccgcct cgacaacctt gacgaactcg
     6301 tcaacgccgc catcgagttc aaacccgaag acagcaactt tgaaaccctg cccgaaaaca
     6361 tttcagacga ccccgccttc cccatcctcg ctttcttgag caacgccacc ctcgaatccg
     6421 gcgaaaatca agcaggtgca ggcgaaaaag ccgtccaact catgaccgtc cacgccgcca
     6481 aaggcttgga attcaacgcc gtcttcctca ccggcatgga agaaggccgc ttccccagcg
     6541 aaatgagcct tgccgaacgc ggcggcctcg aagaagaacg ccgcctcatg tacgtcgcca
     6601 tcacccgcgc ccgcaaacgc ctctacatca ccatggcaca gcaacgcatg ctgcacggac
     6661 aaacccaatt cggcatcgtc tcccgcttcg tcgaagaaat cccacccgaa gtattgcact
     6721 acctgtccat caaaaaaacc gcctacgaca gctatggcaa cacgcgtcaa actgccacgt
     6781 tcaaagacaa aatcatcgac gactacaaac agccgcaaac ctacgcaggc ttccgcatcg
     6841 gacaaaacgt ccgccacgcc aaatttggca ccggcgtgat tatcgatgcc gcagataaag
     6901 gcgaatccgc ccgactgacc atcaatttcg gcaaacaggg cgtgaaagag ttggacacca
     6961 agtttgcgaa attggaagag atgtaaattt gaaatgcagg ctggatactc gtatccgaca
     7021 aaaatatttg acgcgtctat cgtttccgaa aaaccgctgt tggaaaatgt cggattcgag
     7081 aatccgacct atggcaaaaa actgtagcaa ggacaaggca aaaggccgtc tgaatccggt
     7141 aacaacaaat tttgccgcaa ccgccgccgt cattcccgcg caggcgggaa tccagacctt
     7201 tcggcacggg aacttatcgg ataaaaggtt tctttagatt ccacgttcta gattcccgcc
     7261 tgcgcgggaa tgacggacgg taaaagccgg gtatagatac ctaacccttg tttatagctg
     7321 aaaatcaata aggaagaaca ttatgtccca agtttttaaa gattttgatt tgtcctccgt
     7381 atgggaatcc gacagttggg cagatgaaaa ctacaaagaa gccccgttta cccctgaaat
     7441 tttggctgcc gtagaaagtg aactgggcta taaattgccg caaagtttta ttgaattgat
     7501 ggcggtacaa aacggcggaa tatttgtcaa aaactgtttt ccgaccacac agagaaattc
     7561 gtgggcgaaa gatcatgtgc aaatttgcga ggtatcggga atcggttttg aaaaagaagg
     7621 aagcctgtgc ggcgaaacag ggcaaaaact ttggctggaa gaatgggagt atccgcccat
     7681 cggtgtgtat tttgccaacg acccgtcagg cggtcatgcc ctatttgcct tagactatcg
     7741 ggagtgtgga agagacggag agccgaaagt ggtgtttgtc gaacaagaat cggattttta
     7801 aatcgtcgaa cttgcccccg attttgaaac ctttattcac agcttgcggc atgaagatga
     7861 gtttattgac gaggaaatat aaaacggcgg ttgaaaaacc ggaatcatca agggaaaacg
     7921 ggcgaaataa cggataatcg cttgaatccg taaggaaaac ggtttggtgg aacgcgccat
     7981 ccaagacctt tgcaaagaaa aactgtctcc gacagcattg acattattaa cagaacttat
     8041 caagtttgga gctatctcaa atataattcg gttatccttt tgtatccatt aaatcatatg
     8101 cttcaattaa ttgttgttct agctcttgta ccaattttga attgcgaatt cctgacacaa
     8161 tctcaaattc ttctgcatct atgcaaacac ctgcataaat ttcaataaca agggaacgca
     8221 ataattgaag ctcttctctt gttaaagaaa taataatgtc atcacctgtg taattgatta
     8281 tattcataat aattttattt ttgtttgtca aagtaagttt tgcctaaggt tggtctaaat
     8341 gcagttccac catcttttga atttgggtct ctgattacaa ttgctccaga cttatcatcc
     8401 caaattgctc ttatgtgttt ggattgtaat cttcgaattc ccaagaaaaa aatcgtaata
     8461 agtttgaaag tgtcaaatcc caagtttctt ttgagcaata ttctaatatt ttatcaattt
     8521 cacttttaat aatcttatga tcaaactgtt ctaatattaa tgcattagac caaaaaaaac
     8581 cttctttatt acaatgatgg gaaatccatt taggagaaca aatgcaaagt gaaaaaatag
     8641 atgagccttg ttctccttcg attccgatat ccaaatctat ccatctatgg aaattatctg
     8701 gaatttcggg ggtaaatttt tcaaaatcaa tatcatataa atttatgctt tttaaatcca
     8761 atttaatcat tagggctgtc ctagataaat agggaaattc aaattaagtt agaattatcc
     8821 ctatgagaaa aagtcgtcta agccggtata aacaaaataa actcattgag ctatttgtcg
     8881 caggtgtaac tgcaagaaca gcaacagagc ctgacagcat tgtttatacg gattgttatc
     8941 gtagctatga tgtattagat gtgagcgaat ttagccattt ccgtatcaat cacagcacac
     9001 attttgctga acgacaaaac catatcaatg gaattgagaa tttttggaac cgggcaaaac
     9061 gtcatttacg caagtttaac ggcattccca aagcgcattt tgagctgtat ttaaaggagt
     9121 gcgaatggcg ttttaacaac agtgagataa aagttcaaat ttccatttta aaacaattag
     9181 taaaatcgag tttatcttag ttgtccagga cagccccatt atttttataa caccgtgaag
     9241 ccgcacagca gtttgaacag tgatacgccg tttgcgggct tacgagttta ttttcccggc
     9301 ctgcagtttg agcaatacgg tgatttccta cggttaatac aaatgtttac acattgatac
     9361 atttcattta tagttccgcc tatttgaaaa tagaaaatat gaattcgacc gcaagtaaaa
     9421 ccctgaaagg attgtcgctg gtgtttttcg cctctggatt ctgcgccctg atttaccagg
     9481 tcagctggca gaggcttcta ttcagtcaca taggtatcga tttgagttcg attactgtca
     9541 ttatttctgt atttatggtc ggcttgggtg taggtgcgta tttcggtgga cgcattgctg
     9601 accgttttcc ttcaagtatc atccccctgt tttgcatcgc tgaagtatcc atcggtctgt
     9661 tcggtttggt aagcaggggt ctgatttccg gcttggggca tcttttagtt gaggctgatt
     9721 tgcccatcat cgctgctgcc aatttcctct tattgctgct tcctaccttt atgatgggcg
     9781 cgaccttgcc cttgctgacc tgttttttta accggaaaat acataatgtt ggcgagtcta
     9841 tcggtacctt atattttttc aacactttgg gtgcggcact cggatcgctt gccgccgccg
     9901 aatttttcta cgtctttttt accctctccc aaaccattgc gctgacagcc tgctttaacc
     9961 ttctgattgc tgcttcagta tggctgcgtt acagaaagga tggatatagt gaacactaaa
    10021 ccgaatacta gtttgattta tatgctttct ttccttagcg gcttattgag cttgggtata
    10081 gaagtcttgt gggtaaggat gttttcgttc gcagcacagt ccgtgcctca ggcattttca
    10141 tttactcttg cctgttttct gaccggtatc gccgtcggcg cgtattttgg caaacggatt
    10201 tgccgcagcc gctttgttga tattcccttt atcgggcagt gcttcttgtg ggcgggtatt
    10261 gccgactttt tgattttggg tgctgcgtgg ttgttgacgg gtttttccgg cttcgtccac
    10321 cacgccggta tcttcattac cctgtctgcc gtcgtcagag ggttgatttt cccgctcgta
    10381 caccatgtgg gtacggatgg caacaaatcc ggacgacagg tttccaatgt ttatttcgcc
    10441 aacgttgccg gcagtgcatt gggtccggtc cttatcggct ttgtgatact tgatttcttg
    10501 tccacccaac agatttacct gctcatctgt ttgatttctg ctgctgtccc tttgttttgt
    10561 acactgttcc aaaaaagtct ccgactgaat gcagtgtcgg tagcagtttc cctaatgttc
    10621 ggcatcctca tgttcctact gccggattct gtctttcaaa atattgctga ccgtccggat
    10681 aggctgattg aaaacaaaca cggcattgtt gcggtttacc atagagatgg tgataaggtt
    10741 gtttatgggg cgaatgtata cgacggcgca tacaataccg atgtattcaa tagtgtcaac
    10801 ggcatcgaac gtgcctatct gctaccctcc ctgaagtctg gcatacgccg cattttcgtc
    10861 gttggattga gtacaggttc gtgggcgcgc gtcttgtctg ccattccgga aatgcagtcg
    10921 atgattgttg cggaaatcaa tccggcatac cgtagcctta tcgcggacga gccgcaaatc
    10981 gccccgcttt tgcaggacaa acgtgttgaa attgtattgg atgacggtag gaaatggctg
    11041 cgtcgccatc ctgatgaaaa attcgacctg attttgatga atacgacttg gtactggcgt
    11101 gcctattcca ccaacctgtt gagtgcggaa tttttaaaac aggtgcaaag ccaccttacc
    11161 ccggatggta ttgtaatgtt taataccacg cacagcccgc atgcttttgc taccgccgta
    11221 cacagtattc cctatgcata ccgctatggg catatggtag tcggctcggc aaccccggta
    11281 gttttcccta ataaagaact gctcaagcaa cgtctctccc ggttgatttg gccggaaagc
    11341 ggcaggcacg tatttgacag cagcaccgtg gatgctgcag cacaaaaggt tgtctctcgt
    11401 atgctgattc agatgacgga accttcggct ggggcggaag tcattaccga cgataatatg
    11461 attgtagaat acaaatacgg cagagggatt taaccgtctt aaagggtttc aggcaacgca
    11521 ggttttaggt aacgtcctgc tagttcaaaa aaaccgcatc acagcagtcg ggacaaaatg
    11581 gtttaaacat tttgtcccga attcttattc ct
//
LOCUS       DAXHKX010000043        11537 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHKX010000043 DAXHKX010000000
VERSION     DAXHKX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11537
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.043"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(1..1182)
                     /locus_tag="ACFMWZ_000952"
                     /pseudo
     CDS             complement(1..1182)
                     /locus_tag="ACFMWZ_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1328..2032
                     /locus_tag="ACFMWZ_000953"
     CDS             1328..2032
                     /locus_tag="ACFMWZ_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC5934931.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFYVSRERRNEVIDQVSKDAILRFK
                     ARADKLSGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(2097..2663)
                     /gene="dcd"
                     /locus_tag="ACFMWZ_000954"
     CDS             complement(2097..2663)
                     /gene="dcd"
                     /locus_tag="ACFMWZ_000954"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC5934932.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2729..3145)
                     /locus_tag="ACFMWZ_000955"
     CDS             complement(2729..3145)
                     /locus_tag="ACFMWZ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC5934933.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3197..4096)
                     /locus_tag="ACFMWZ_000956"
     CDS             complement(3197..4096)
                     /locus_tag="ACFMWZ_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC5934934.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4127..5584)
                     /gene="der"
                     /locus_tag="ACFMWZ_000957"
     CDS             complement(4127..5584)
                     /gene="der"
                     /locus_tag="ACFMWZ_000957"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC5934935.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5734..6363)
                     /locus_tag="ACFMWZ_000958"
     CDS             complement(5734..6363)
                     /locus_tag="ACFMWZ_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC5934936.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6364..7659)
                     /gene="hisS"
                     /locus_tag="ACFMWZ_000959"
     CDS             complement(6364..7659)
                     /gene="hisS"
                     /locus_tag="ACFMWZ_000959"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC5934937.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7777..8433)
                     /locus_tag="ACFMWZ_000960"
     CDS             complement(7777..8433)
                     /locus_tag="ACFMWZ_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC5934938.1"
                     /translation="MMKFKYVFLLACVAVSLSYRLNAAPMFNDNPVVYGKIKVQSWKE
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLEAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVEQIRQARAE
                     "
     gene            complement(8491..8682)
                     /locus_tag="ACFMWZ_000961"
     CDS             complement(8491..8682)
                     /locus_tag="ACFMWZ_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934939.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENDTKITAEIGSAWHFLDAACWISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(8715..9251)
                     /locus_tag="ACFMWZ_000962"
     CDS             complement(8715..9251)
                     /locus_tag="ACFMWZ_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934940.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9419..9577)
                     /locus_tag="ACFMWZ_000963"
     CDS             complement(9419..9577)
                     /locus_tag="ACFMWZ_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934941.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLERTGAEVYINGRRVYHNEA
                     EMASASLR"
     gene            complement(9860..10153)
                     /locus_tag="ACFMWZ_000964"
     CDS             complement(9860..10153)
                     /locus_tag="ACFMWZ_000964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934942.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLK"
     gene            complement(10211..10576)
                     /locus_tag="ACFMWZ_000965"
     CDS             complement(10211..10576)
                     /locus_tag="ACFMWZ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934943.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(10558..11103)
                     /locus_tag="ACFMWZ_000966"
     CDS             complement(10558..11103)
                     /locus_tag="ACFMWZ_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934944.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKILPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(11191..11415)
                     /locus_tag="ACFMWZ_000967"
     CDS             complement(11191..11415)
                     /locus_tag="ACFMWZ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934945.1"
                     /translation="MMKNKYILTKAAIGITAISIFYLLNQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAIIAAHFIFVALSD"
BASE COUNT         2847 a         2721 c         2768 g         3201 t
ORIGIN      
        1 ggtattttct taccgtattc tgcgattgcc tgtcggaaaa tgccgatcaa cctgcctata
       61 acggcatttt cgccaaattc gttcagacag ttttccctaa gtcgggcagg ttcaaaatca
      121 gagtggtgtt catacatttt gatgagtgcg tcggcaaggg catcgtcgtc gtcaacagga
      181 accaaatatc cgttgccgtc tgaaacaata gattccgcac cgccgcagcg tgttgcaatg
      241 acgggcaatc cttgggacag tgcttcgata tagaccacgc cgaaggtttc ggtgcggctg
      301 gcaaggacga atgcgtcgct gttcctcatc aaatccaaga ctgcttcggg ctgcaatgcg
      361 cccaaaaatg taacggcatg ggtaatgccc aagtctgccg cctgctgttt cagccgctgt
      421 tcttcctgtc cgctgccgcc gatgtttaga cgcagttgcg ggcattgtgc caacgcccgg
      481 gcaaaggcag tgagcaggac atcgtgtcct ttgagacggc gaaggtgcga gacggtgcag
      541 aacacgaaat gcggtttgtt gtttttttct ctttgttcaa acggtcggtt gaatattccg
      601 cccagtatgt tggggaggta ctgccattcg cagccgtatt tgtgttgcag gacgtgtgcg
      661 aaatggcggc tgatggcgag acgtgcggcg gcgtgcgccg ccgcattttt cataggctgc
      721 cattggtgcg ggcgcaccaa accgcgcgta atggtgctgc tgtgttccgt gacgacatag
      781 gggatgccgt atttttggga aattttttgg gcgagtatgc cggcatagtt catgcggtgg
      841 gcgtgaatca ggtcgggcag cccgttttcg cggatgtagt gtttgaaggc tttcaaaccc
      901 acgcgcaccc agcggatgcg gtcgatgtcg atgaacggaa agcgggggaa gaaatacatg
      961 ccgtgccatg catagatgtc caaaccgctt tgccgatatt ttgcaaaacc gtaaggtcgg
     1021 tcaggatgct tgctgtttct ttccgcaagt agcggaacat cggtgcaagc acggcggttt
     1081 tgatgccttt cctctgcaat gccagtgcct gattttgaaa aaaatcccgt ccacatcctg
     1141 ttcggattgc ggataccatg aggggatgac gaggacgtgc aagggtttgg gcatagtggg
     1201 attccgtatc ggaaaggcgg ttattataag acagacgcag accgaatatt taaattgttg
     1261 ccttacgcta atgcaattta gcgcgccgat atgctagatt ggcggtttta tgagtgagga
     1321 tacggatatg ttgcgttcta ttttggcggc ttccctattg gcggtatctt ttccggcagc
     1381 ggctgaagca ttgaattaca atattgtcga attttccgaa tcggcgggtg tcgaggtggc
     1441 tcaggataca atgtccgcac gtttccaagt ggcggcggaa ggacgggaca aaaatgccgt
     1501 caatgccgag tttgttaaaa aattcaacag ttttaacaga aaatcgaaaa acggcagctt
     1561 taaaaccgaa ttggtatcgc gcagtgcgat gccgcgctac cagtacacca acggcagacg
     1621 cattcaaaca ggttgggagg agcgtgcgga atttaaggtc gaaggtaggg attttgatgc
     1681 gttgaaccgt tttattgccg atgttcaggc agatgccgcg ttggaatata cggattttta
     1741 tgtgtcgcgc gaacgccgaa acgaggtcat cgatcaggtc agcaaggatg ctattttgcg
     1801 tttcaaggcg cgtgccgaca agttgtcggg catattgggt gcgtccggtt ataaaatcgt
     1861 caaattaaat ttgggacaca tcggcagcca tatcgcggga gggggagctg ctcaggcaaa
     1921 aatgcttcgt gccatgccga tggcggcaag cgtcaatatg gagggtgcgg attctgccgc
     1981 gcagggtgtg gaggaaatca gcatcagcgt caatgggacg gttcagttct gatttgaggt
     2041 gaacggtaaa tgccgtctga aacccgacga taagggttca gacggcattt tgtatttcag
     2101 gctttgggca gggttacgcc ggtttgcccc atgtatttgc cgttgcggtc tttgtaggaa
     2161 gtttcgcaga tttcgtcgct ctcgaaaaac aggacttgcg ccacgccttc gccggcgtag
     2221 attttggcgg gcaggggggt ggtgttggaa aactcgaggg taacgtagcc ttcccattcc
     2281 ggctcgaacg gggtaacgtt gacgatgatg ccgcagcgtg cgtaggtgga tttgcccaag
     2341 cagacggtca ggacgttgcg cgggatgcgg aaatattcga ccgtgcgcgc cagtgcgaag
     2401 gaattgggcg ggatgatgca gcagtcgtct tcaacggtaa cgaagttttt cgggtcgaag
     2461 tttttgggat cgacgatggt gctgttgatg ttggtgaaga ttttaaattc gtttgcgcag
     2521 cggatgtcgt agccgtagct ggacgtaccg taggagatga tgcgtttgcc gtcggcttct
     2581 ttgatttggt tcggctcgaa agggtcgatc atgccgaatt cttcgctcat tcggcgtatc
     2641 catttgtcgg acttgatgct cataatgttt tccttgtttc ttgcagtgtt cggacaaagc
     2701 attgggggat gccgtctgaa aacggggctt atttgttttt gggcagcttc acttctttaa
     2761 tcatgccgtt ttcgcatttc ataatgaaga gggtttcgcc gttttgcgag acggtaaatc
     2821 tgccgtgtgc caagcctttt ttgaatgtgc ccgagagtac catgttgcgg aatttggtac
     2881 tgtcggaatt gaacggttcg ataaatattt cgcggttggc ggcaacggta taaacgcctt
     2941 gtccgtcgaa tttgccattt ttaaacgaac cggtatagtt gcgcccgtct tggcagcgcc
     3001 atgtgccttt gccggcgggt ttaccgtctt tgccgacatc gccttcgtag gtgcagccgg
     3061 gttcttgata ggaagtcaga acggcggacg cggcggggag ggcgaacatc atggcgggca
     3121 gtaggaatgc gagatgttta agcataaggg ttattccatt ggattttggc tgacggtata
     3181 aaagccgtct gaaaaatcaa tcttgccagc cgcccaaata ggaaaccagc tcttccaaca
     3241 tgatgctgac ggattccgcc atcaggattt gcgaggcgaa ggcgagaccg gcggcatcgt
     3301 cgccgttgct ttcggcttct tcctgcaata cgtcgaggta ttggatgcgc ttgagtgtga
     3361 agtcttgcgt gaggataaag gcaatttgtt cgcgccacac caagcccagt tgggtaacgg
     3421 ttttaccgtt tttgacgtgt tgaaccactt cgtcggcggt taaatcttgt ttggatactt
     3481 tgacgacggg aacaatatcg cccgtgcctt tgagttcgca atcgctgtcc aattcaaaac
     3541 cgccttcgca atgcccttgc aacagccagc cggtcatcag ggaagagggc gattgtttgg
     3601 tattcggcag cgaggcttcc aaaccgccca aagcttcgcg cagcttggtc aggatgtttt
     3661 ccgctttggc ggaagccgcg ttattgacga gcaggtagcc gtggcgggtg ttaaacaccg
     3721 cttctgtacg gctgctgcgg gtaaacgctc ggggcagcag gtcgtctgta atttgctctt
     3781 taagctcttg tttttctttg cgaccgacat tgcgggcttc attgttttgg atttccgcta
     3841 ccttctcttc caaaatatcg cggatgacgc cggcaggcag gactttttcc tcttttttca
     3901 gggcgacgcg caaggtaaag tcggcaggaa aaacgagttc cggggagaat gaaaccggtg
     3961 cggtaaagcc ttcgctgaac cagtctaagc cttggcaatg ggtaaattca gcttcagcaa
     4021 gtttgtcggc aagtacgtcc gcctcaggca gcttttcttt gttgagcgga taaaaactaa
     4081 tctgcttgaa ccacataatg tttcctgttg tttgaaatgt cgggaattat ttgctgaatt
     4141 gttttttcac actgactttg gttttcttct tgaagcggtt tttctcttct ttgcggcctt
     4201 cgcgtttctc aatacggttg ctcaggctga cgcggcgcag cggttttttc ttcggtttgt
     4261 cgtcggcgtt ttcatacggg ttttccgaaa cattgtattg aatccgcaac ggcgtgcctt
     4321 gcaggttgaa ggctttgcgg aacgtttggg tcagatagcg cgtatagctg tcggaaatcg
     4381 cgtgcagcga attgccgtgt accacaatta cgggagggtt catgccgcct tggtgggcat
     4441 aacgcatttt cggacgcacc aagccggcac gcggcggttg ctgacgctcg attgcgcttt
     4501 gcaggacgcg cgtgattttc ggcgtcggca tcttaatcat cgccgcgttg taggcagcct
     4561 gaatgctgtc aaacaaaccg tctataccgc gctctttcaa tgcggaaata aagtggaact
     4621 tggcaaaatc gaggaaatac agtttgcggt tgatatcgcg tttcacttgc tcgcgacgtt
     4681 cttcgctgat gccgtcccat ttattgaccg ccaccaccaa agcacggcct gcctccaaag
     4741 cgaaacctgc aatcgtcgca tcttggtcgg cgatgtcctg ctgcgcgtcc aataccaaaa
     4801 cagcgacgtt tgccgcttca accgcctgca tcgctttgat aacggagaat ttttccactg
     4861 cttcatccac tttgccgcga cggcgcacac ctgcggtatc gatgatggta aacggtttgc
     4921 cttcgcgctc gaaatcgata tggatactgt cgcgcgtcgt gccggccata tcgaaggcaa
     4981 tcacgcgctc ttcgccgaga atggcgttta ccagcgtgga tttgccgacg ttcggacgac
     5041 cgatgacggc aaaaacggga tgttttgcat cggcttcttc gacttccggc tcggggaagt
     5101 tttctaaaat ttcttcaatc aggtaataca cgccgtcgcc gtgcgcaccg gaaataacat
     5161 aagggtcgcc caaagcaagt tcgtagaact cggcggcaag cacggccctg ttgccgcctt
     5221 cgcctttgtt taccgccaga taaacagggc gcgggctttg gcgcaaacgg tcggcaatga
     5281 ttttgtcttg cggggtcaga ccggtgcggc cgtccaccaa aaatacgacg gcatcggctt
     5341 catcgacagc ctgcaaggtt tgttttgcca tttcgtgcaa aatgccgctg tccacaaccg
     5401 gctcgaaacc tccggtatcg atgaccaaat aaggtttgct gccgactttg ccgtgtccgt
     5461 aatggcggtc gcgcgtcaga ccgggcaggt cgtgtacgag cgcgtctttg gtgcgcgtca
     5521 aacggttgaa taaagtagat ttgccgacgt tggggcggcc gacaagcgcg atggttggtt
     5581 tcatgattgg gtctttctgt gtcaagtgcc gttcgggaga actgaacaca agcaggtgtc
     5641 cgttgggaca cggcagatgg ttttacggga attgccgtag gatagtgttg tctgaaatgc
     5701 cgtctgaaga gagggtggca tttcagacgg catttatttc agcgaatcga gtttcatttg
     5761 aaccaattca cgaccgacag aatcttgagg cattttttct aaagcctgac cgtagttttt
     5821 taaggcttcc tggctttttc cctgtgcggc atagacatcg cctttggttt ccatcagcag
     5881 gggggagaag tccgcttcaa ccggcgtgtc gagcgcggca agcgcggcat cgtatttttt
     5941 ttgttgcaac aacacaacgc ccagacgctg cgccgccaac gcttgaatca ggctgtcttt
     6001 ttggttggac aacacccatt tcaaatggcc ttcggcaaca tcgtaacgct gcgcgtcaaa
     6061 ttcggttgcc gccgccatca gcgtggcttg ggcggcggaa atagaatgcg ggtagctttg
     6121 ttggagtttg gtcaattcgg cattaatttc gctttgcggg gctttgtttt gcgccttttc
     6181 cacgatgttt gccagcaccg ccgccgcttc ctgattttgg gaagccgcac ggttttggta
     6241 aaccgtgtat cccaagtagc cgagtgctgc caaaatcagc agggcaaaca gccatttgcc
     6301 cgtggttttc caaaaatatt taaagttgtc taactcttgt tgttcttcga gatgggctgc
     6361 catttatgcg ttcttccatt gttgtaaagt atgggttaaa tcctcggcgg cgacagtttg
     6421 ctgaccgtgt gcgccgttca tgtctttgag cgtaaccgta ccgttcgcca gttcgtcttg
     6481 cgcgacaatc agggcaaagc gcgcgccgct gttgtcggct tttttcattt gcgctttcag
     6541 gctttgatag ccggaatgct gcattacatt gaaaccttgc gcgcgcaagg cttgcgcgta
     6601 tttcatcacc tgcaagtccg ccctttcgcc ttggtgcatt gcatagacat caggcgcagc
     6661 gttcacttcc agcgagccgt attcgctcac caaaagcagc agccgttcga tgcccatcgc
     6721 aaagccgatg gacggcgcag gtttgccgcc aagttcctca atcaaaccat cgtaacggcc
     6781 gccgccgcac acagttgcct gcgcgccgag tttgtcggtc gtccactcaa aaaccgtctg
     6841 attgtaataa tccaaaccgc gaaccaagcg cggattttca atatattgaa tacccaaacc
     6901 atccaacatc gccttgaagc gtgcatagtg gttttgcgaa gcctcgccca agtaatccac
     6961 caaacgcggc gcggcgttgc agatttcctg caaatcaggg tttttcgtat ctaaaacgcg
     7021 caaaggattg gttttcagac ggcgtttgct gtcttcatcc aatttatctt cataacgggt
     7081 cagatattca accaatgccg cacggtgtgc cgcgcgttcc tcacggttgc ccaagctgtt
     7141 gatttccaaa gtcaggtatt cgcgaatacc caatttttcc cacaagtcgg cagacatcgc
     7201 gatgatttcc gcatcaatat ccggaccttc aaaacccaaa gcttcgatac cgacctgatg
     7261 gaactgacga taacggcctt tttgcggacg ctcgcggcgg aacatcggcc ccatatacca
     7321 cagcttttgc gggctgttgt agagaaggtt gtgttcgacc accgcacgca gacaagaagc
     7381 cgtaccttca ggacgcaggc tcaaactcaa agaatcgttt gaatcggaaa aggtgtacat
     7441 ttccttgccg accacatcgg tttcttcgcc gatggagcgg acaaacaaac cggtttgctc
     7501 gacaatcggc gtacggattt gctggtagcc gtaagcgcgc gtccaacggt ttaccgtatc
     7561 ttcaaacgcc tgccaaaacg cagccgtcag tttgaaatct ttttgttcga caggtaaaag
     7621 gtcgttcata ccttttacag attgaatttt ttgtgccatt tcaatatggg ataagcgaaa
     7681 atcaaatagt tgggcattat agctgatttt taagggtttg tgaggttggg agtaaatcaa
     7741 aaggccgtct gaaacagttt tcagacggca tttgacttac tctgcacgtg cttgcctgat
     7801 ttgttcgact gcaaactccg tctgccgctt gggatgatgt gtgaaaaaca atttatttga
     7861 aattgtctcg gcttttttcg gcacgaccgc caaaatctta cctgccaaat ttccctcacg
     7921 ggtttgccaa gcctccaaaa actgcgccct gctcattgaa acatgcccca gcgacgggtc
     7981 ggcaagcaaa accgtattgc cgtctatccc gcgcaatacc gagaaatgat catccttgcg
     8041 gtatttcaga tacacgatga cggggatttt caactgcgcg agctgctcga aagacagggc
     8101 atagcctttc gcttcaaaac ccaaatcagg cataatgcgc cgcatatcct caaacgacgc
     8161 gcgcatctgc tccttatcca gctttttcaa cacttcttct tccgtcagcg tttgcccgta
     8221 aaaattgttc aaaagcgtcg ccaccgaagc cgccccgcag gaaaaatcca aatcctgctt
     8281 tacaatattg aaatcgcgcc tctctttcca actctgcact ttgatttttc cgtaaacaac
     8341 aggattatcg ttaaacatcg gcgcagcatt caaacgataa gataaagaaa cggcaacaca
     8401 cgccaacaga aaaacatatt tgaacttcat catattttcc acataaaggg cagcctgaaa
     8461 atctttcagg ctgcccttgt caaattattc ctagctttcg gcttttttgg caaaccaaac
     8521 aatccgatta cccgcataat actttccatt tattgaaatc caacaagccg cgtccaaaaa
     8581 atgccatgca ctgccgattt ccgcagtaat ctttgtatcg ttttcttcaa attccaaata
     8641 ttcacccaat aataaacttg aaacagaacg cgtgggcagc aagtgcccac cctacgctta
     8701 ttcaaataat ttgattaaat aaagaataaa gaaaatgcat caggaacaaa attataatct
     8761 gccacctgac tcacaccgct tttaaagtaa ggggcatcaa aatcaaaacc gcaaaaaaaa
     8821 taatttttgc attgattttt aatagattta aaattcgaat atagtgtttc tccagattta
     8881 aaaagtttat ctttatctat atatttgatg ctttccctat ccaaaataaa tatttcaaac
     8941 attaaaaaat cattacatga ccaagccaaa tcataaattt gattcaaatg ggtatcataa
     9001 agataaaaat aataaggttt gggaacaggt aaaatattta atggaaaagg aggactaatt
     9061 ttcttaatat ctgatgactg atatccatat ctttctatat tcttaaaaat ttcatctttc
     9121 ttaagataac aagacattct gacaatcaca acctgttcat cggatatatt atctatttgc
     9181 atggcgcgta taacacgcca tgcctgatta aaattagtct cccttacctt agaatctatt
     9241 atcttttcca aagatgaaaa tgccccttat cttgaactct ccagttagaa cctggaagtt
     9301 tcgtacctct taaatgtgta ttaatatttc ttatagtttc attaaaatgc cacgcgctgc
     9361 cgatttcaac ggtaattttc gtaccgcttt cttcaaattc caggtattcc cccattagct
     9421 aacgcaaaga agcagacgcc atttcggctt cgttatgata aacccgcctt ccgttgatat
     9481 agacttccgc ccctgtccgc tccaaattcc aaaaattccg tactgaaatt tccatatccc
     9541 gatattgtgc agaccatgtt ttttcgaagg ttttcataaa atttcctata cctgtccaat
     9601 cggcacatat caattgcatt attatagtgg attaacaaaa atcaggacaa ggcaacgaag
     9661 ccgcagacag tacaaatagt acggaaccga ttcacttggt gattcagcac cttagagaat
     9721 cgttctcttt gagctaaggc gaggcaacgc cgtactggtt taaatttaat ccgctataca
     9781 tctcaatacg ataaatattt cttaagtcaa aatgcaagcc tgaccgtacc ttaactgtca
     9841 aaattttatt attttttatt tattttaaaa caatttctgc aaaattctct tcgctttctc
     9901 tcttttttag aagcacataa gaaaaaataa aacttccccg attaaattca taaatatgtt
     9961 tcaaccattt gcctcctctt tctgtaagac aagattcagt ttcattcttc cttattgtat
    10021 aaatatttcc ttcacaaaat ctgaaataaa tccataaatc catcttatcc ataattaaag
    10081 aaaaagtttc acctcgagat tttgtcaaca attcgcaagg ttgcgatgtt gcaatcaaat
    10141 agccgaaaga cattttttac ctcatacatg gtcgaaatca gtttctgtta gttcagaatc
    10201 cattttttcg tcaacaactg aatccgcatt tttgaattaa cgttttcatc agctgccgtt
    10261 tatctaaacc ggcaggttca gtttcaaaat aagccttata tgaagactgt aagcatttca
    10321 gaaaaagatc atcagaagac atatctgccg aatcaaatac aactgttttg attttggtac
    10381 ttacccaaaa ccctttttgc tctttttcta ctatacggaa attcagaata tttccaaccg
    10441 aatcaaaagc acggtaaaca tcatccatca aatcctgcgg ctctattttc ttttccaatt
    10501 ccgacaatcc ttgaaatata tccaaagaca catcttcaaa tagaaaaaaa ggaggagtta
    10561 gaagcggttt ttccatgatc tgtccgtaga ttttgattcc caagggcgat gacgaccaat
    10621 tccctgtcca ggcaaaatct tgcccgtatt atccgtaact cgacgatgat aatggggaaa
    10681 ttttccaata ggatgacctg ttctattacc gaaaggggct atccgcatat tattgccgat
    10741 tttaatctca cgtccatatt tagcaaagga aacaaccttt cctgcagcac ctacaccacc
    10801 aggaattgcg cctaatccgc cagcaatagc aacatctcta acagaagctg gtctgcctgt
    10861 cgttgcataa ctaaaaccat gctgtgtcca cataccaatg gcagcaccac ccaagatagc
    10921 caatggaagg aacgccccct ctgtctcctt catctccttt tgagaaagct ccgccaactg
    10981 catcggtgca tctgcccgcg tgtggaacac ttggtcttca aatgcctgat tgtccaagcc
    11041 gtttgccatt gcgggggcga ttatagacag catcatcgtg gcttcagtga tttgtttttt
    11101 cataacaaat tcttagggag gagataatgt tccatcaaaa tcctcctgaa aggctgcctg
    11161 aaatttccaa gcagcctttt tgtttgctta ttagtcggat agtgcgacaa atataaagtg
    11221 ggcggcaatt attgccgtaa cggcagccat aactgtttta ttttcttcaa accaaagcaa
    11281 gttgagaaac ataaacatca agaaatatga tggttcttca gttttgccgc tgctttcttg
    11341 atttagaaga taaaatatag atatggcggt tatgccgatt gctgcttttg ttaaaatata
    11401 tttgtttttc atcatcttgt atggtttttc atgcaagttt caacaataaa attcggaata
    11461 agatagagag atgttgatat atcagataaa tatatagtgg attaaattta aaccagtaca
    11521 gcgttgcctc gccttag
//
LOCUS       DAXHKX010000044        11441 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHKX010000044 DAXHKX010000000
VERSION     DAXHKX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.044"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..553
                     /locus_tag="ACFMWZ_000968"
     CDS             <1..553
                     /locus_tag="ACFMWZ_000968"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFC5934946.1"
                     /translation="AWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPV
                     YGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICTLLEELV
                     PEKPAGVARYEDLITFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYL
                     DNKTWWQNVLNGSYRLERLGTGK"
     gene            616..1482
                     /gene="rfbA"
                     /locus_tag="ACFMWZ_000969"
     CDS             616..1482
                     /gene="rfbA"
                     /locus_tag="ACFMWZ_000969"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC5934947.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQDLQIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            1536..2078
                     /gene="rfbC"
                     /locus_tag="ACFMWZ_000970"
     CDS             1536..2078
                     /gene="rfbC"
                     /locus_tag="ACFMWZ_000970"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC5934948.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWVGEILS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPKAEHSLIWNDPTVGIDWPLQGE
                     PNLSPKDLAGKVLAEAVTFG"
     gene            2134..3255
                     /locus_tag="ACFMWZ_000971"
     CDS             2134..3255
                     /locus_tag="ACFMWZ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stealth family protein"
                     /protein_id="HFC5934949.1"
                     /translation="MNLNKIRKFFRESHIFFRDFLNKRFPPYNVEQPIPETEEFTLIN
                     ADANLASLTENVLPNFPIDVVFTWVDNTDKAWQEQYYRTLQPIDQEDIGLYATDPARF
                     SNHNELFYSVQAVQKFMPWVRNIFIVTADQKPKWLDENIHSKIKLINHSQLIDAKYLP
                     TFNSHVIEANLYKIPDLSEHFIYFNDDVFVARPLMPNHFFENNGLASLFVANKSFQKM
                     RQRGLITPTLTASEHALKLLKRHYPTININTPLVHTYVPLRKTSFQKAWSLFEDEINS
                     FLNNKVRHNSELNMASFLIPWLMYLDGYATPKREICYYFNIRSSHAQTQYKKLLFEKE
                     HLHMPHSFCINDSSSNNADKNYALHFRNFMDTYFEIETE"
     gene            3300..5324
                     /locus_tag="ACFMWZ_000972"
     CDS             3300..5324
                     /locus_tag="ACFMWZ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC5934950.1"
                     /translation="MIFSTFGLFKQRLLLTKFLDKISTDKTALFWGKKHPKRRKLAHI
                     LRLSLLNLEDGFLRSLGLGVAGYPPYSIVYDDIGIYYDTTRPSRLEQLILAADTMPSE
                     TLAQARQAMDFILQHHLSKYNHAPELSDDHPLRSPSKPETVLIIDQTFGDMAIQYGGA
                     DASTFELMFQTALNENPQADIWVKTHPDVLCGKKQGYLTQLAQQHRVHLLAEDINPIS
                     LLQNVDKVYCVTSQMGFEALLCGKPLTTFGLPWYAGWGVSDDRHPEINRLVQTQRRAT
                     RNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELYCVGMSLWK
                     RAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAEQHHIPLLR
                     MEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAETPSRLEYILQNQNFDDQDFQTALKL
                     QKMLTENHISKYNVGSSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYRNLDLLRTV
                     RERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQYADEIHTMT
                     SLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGTLIHYPDYI
                     HPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK"
     gene            5461..6720
                     /locus_tag="ACFMWZ_000973"
     CDS             5461..6720
                     /locus_tag="ACFMWZ_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC5934951.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNLRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPTT"
     gene            7090..8496
                     /locus_tag="ACFMWZ_000974"
     CDS             7090..8496
                     /locus_tag="ACFMWZ_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC5934952.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            8714..9928
                     /gene="gltS"
                     /locus_tag="ACFMWZ_000975"
     CDS             8714..9928
                     /gene="gltS"
                     /locus_tag="ACFMWZ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC5934953.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            10027..11043
                     /locus_tag="ACFMWZ_000976"
     CDS             10027..11043
                     /locus_tag="ACFMWZ_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC5934954.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            11054..11251
                     /locus_tag="ACFMWZ_000977"
     CDS             11054..11251
                     /locus_tag="ACFMWZ_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934955.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            11269..11418
                     /locus_tag="ACFMWZ_000978"
     CDS             11269..11418
                     /locus_tag="ACFMWZ_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934956.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTVGFSCVSYADVSTHRTIQKAS
                     PYVPS"
BASE COUNT         3036 a         3175 c         2590 g         2640 t
ORIGIN      
        1 cgcgtggttg cgtacttacg gcttgccgac cattgtaacc aactgctcca acaactacgg
       61 tccttaccat tttccggaaa aactcattcc tttgatgatt ctgaacgcgc ttgacggcaa
      121 accgctgcct gtgtacggcg acggtatgca aatccgcgac tggctgtttg tcgaagacca
      181 cgcgcgcgca ctgtatcagg ttgttaccga aggtgttgtc ggcgaaacct acaatatcgg
      241 cggccacaat gaaaaagcca atattgaagt cgtcaaaacc atctgcaccc tgctggaaga
      301 gcttgttcct gaaaaaccgg ccggtgtggc gcgttatgaa gatttgatta ctttcgtaca
      361 agaccgcccc ggccatgacg tacgctacgc cgtcgacgca gccaaaatca ggcgggattt
      421 gggctggctg cctttggaaa ccttcgagtc cggcctccgc aaaaccgtgc aatggtatct
      481 ggacaacaaa acctggtggc aaaatgtatt gaacggcagc tatcgtttgg aacgtttagg
      541 tactggaaaa tagttttcag acggcatccc gacgcaatgc cgtctgaaaa cccatcgcaa
      601 aggaagaaag aaaagatgaa aggcatcata ctggcaggcg gcagcggcac gcgcctctac
      661 cccatcacgc gcggcgtatc caaacagctc ctgcccgtgt acgacaaacc gatgatttat
      721 taccccttgt cggttttgat gctggcggga atccgcgata ttttggtgat taccgcgcct
      781 gaagacaacg cctctttcaa acgcctgctt ggcgacggca gcgatttcgg catttccatc
      841 agttatgccg tgcaacccag tccggacggc ttggcacagg catttatcat cggcgaagaa
      901 tttatcggca acgacaatgt ttgcttggtt ttgggcgaca atatttttta cggtcagtcg
      961 tttacgcaaa cattgaaaca ggcggcagcg caaacgcacg gcgcaaccgt gtttgcttat
     1021 caggtcaaaa accccgaacg tttcggcgtg gttgaattta acgaaaactt ccgcgccgtt
     1081 tccatcgaag aaaaaccgca acggcccaaa tccgattggg cggtaaccgg cttgtacttc
     1141 tacgacaacc gtgccgtcga gttcgccaaa caaatcaaac cgtccgcacg cggcgaattg
     1201 gaaattaccg acctcaaccg gatgtatttg gaagacggct cgctctccgt tcaaatattg
     1261 ggacgcggtt tcgcgtggct ggacaccggt acgcacgaaa gcctgcacga ggcggcttcg
     1321 ttcgtccaaa ccgtgcaaaa catccaagac ctgcaaatcg cctgtctgga agaaatcgcc
     1381 tggcgcaacg gttggctttc cgatgaaaaa ctggaagaat tggcgcgccc gatggcgaaa
     1441 aaccaatacg gccaatattt gctgcgcctg ttgaaaaaat aatgtttgag gccgtctgaa
     1501 acttttcaga cggcctttag atgaaagata aaaagatgaa catcattgat accgccattc
     1561 ctgacgtaaa actgcttgag ccccaagtct tcggcgacgc gcgcggcttt tttatggaaa
     1621 ccttccgcga cgagtggttt aaaacccaag tctgcgaacg caccttcgtg caggaaaacc
     1681 actccaaatc cggcaaaggc gtattgcgcg gcctgcacta ccaaaccgaa aacacacaag
     1741 gcaaactcgt acgcgtagtt gtcggcgaag tgttcgacgt ggccgtcgat atgcgcgaag
     1801 gttcgccgac tttcggcaaa tgggtaggcg agattctgtc cgcgcaaaac caatgccagc
     1861 tttggatacc cgaaggcttc gcgcacgggt tttgcgtttt gggagacgcg gcggaagtcg
     1921 tgtacaaatg cacggactat tacaacccca aagccgaaca ctcgctgatt tggaatgatc
     1981 cgaccgtcgg catcgactgg ccattgcaag gcgagccgaa cctgtcgcct aaagacttgg
     2041 caggcaaagt attggccgaa gcagtaacgt ttggataact tcaaaacata acagaatcaa
     2101 ctgtttctat tttgtagtat tagaaagttt caaatgaatt taaataaaat tagaaaattt
     2161 tttagagagt cccatatttt ctttagggat tttctaaata aacgctttcc tccttataat
     2221 gtagaacagc ctattcctga aactgaagaa ttcactctga tcaatgcaga tgccaatctt
     2281 gcatcactca cagaaaacgt tttgccaaac tttcccatcg atgttgtttt tacttgggtc
     2341 gacaataccg ataaagcatg gcaagaacag tattaccgta ccctgcagcc catcgatcag
     2401 gaagatatcg gcctgtatgc gaccgaccct gcccgctttt ccaaccataa tgaacttttt
     2461 tattcagtac aggccgtaca aaagtttatg ccgtgggtcc gcaatatttt tattgtaacg
     2521 gcggatcaaa aacctaaatg gttagatgaa aacatccatt ctaaaattaa attaatcaat
     2581 cattcgcaac ttattgatgc caaatactta ccaacgttca attcccacgt cattgaagca
     2641 aacttatata aaattccaga tttaagtgaa cattttattt atttcaatga tgatgttttc
     2701 gttgcaagac cactgatgcc taaccacttt tttgaaaata atggtttggc ttccctattt
     2761 gttgccaata aaagttttca aaaaatgcgt caacggggtc taatcacgcc gacattgacc
     2821 gcatcagaac acgcattaaa gctcttaaaa agacattatc cgacaattaa tatcaataca
     2881 ccgttagtac atacttacgt tccgctacgg aaaacatcct ttcaaaaagc atggtcatta
     2941 tttgaagatg aaataaattc tttcctaaat aacaaggttc gccataattc tgaacttaat
     3001 atggccagtt tcttgatacc ctggttgatg taccttgacg gatatgcaac accgaaacgt
     3061 gaaatttgct actatttcaa tatacgcagt tcacacgccc agactcaata taaaaaatta
     3121 ttatttgaaa aagaacatct tcatatgccc cactccttct gcattaacga ttcaagcagt
     3181 aataatgcgg ataaaaacta tgcccttcat ttccgtaatt ttatggacac ttactttgaa
     3241 atagagacag aataaatttt tacattaatg atacttaata gataacataa taatttaaaa
     3301 tgattttttc aacattcggc ctatttaaac aacgtctctt gctaaccaaa tttttagaca
     3361 aaatttcaac ggataagacc gcattatttt ggggcaagaa acatcccaaa cgtagaaaat
     3421 tagcacatat cttaagattg tccttgctca atcttgaaga cggcttttta cgatcgctcg
     3481 gactgggtgt cgccggctat cctccctatt ccatcgtcta tgacgacatc ggcatctact
     3541 acgacaccac ccgtccttcg cgtttggaac aactgattct tgccgccgat accatgccgt
     3601 ctgaaacctt ggctcaggcg cggcaggcga tggatttcat cctgcaacac cacctgtcca
     3661 aatacaacca cgcgcccgaa ctttcagacg atcatccttt acgctcccca tccaagcctg
     3721 aaactgtcct cattatcgac caaaccttcg gcgatatggc catccaatat ggcggcgcag
     3781 acgcctctac gtttgaactg atgtttcaga cggccttgaa tgaaaacccg caagccgata
     3841 tctgggtaaa aacccatccc gatgttttgt gcggcaaaaa acaaggctat ctgacccaac
     3901 tggcgcagca acaccgcgtc catcttctgg cagaagacat caacccgatt tctttgttgc
     3961 aaaacgtcga taaagtttat tgcgttacct cgcaaatggg ttttgaggca cttttgtgcg
     4021 gcaagccgct gaccaccttc ggcctgccgt ggtatgccgg atggggtgta agcgacgacc
     4081 gccatcctga aatcaaccgc cttgttcaaa cccaacgccg cgccacccgc aacttgctgc
     4141 agctcttcgc cgcagcctat ctgcaataca gccgctacct caaccccaat accggcgaag
     4201 caggcagcct ctttgatgtc atcgactatc tggcgacggt caaacgtaaa aacgacaaat
     4261 tgcgtggcga gttatattgc gtcggtatgt ctttgtggaa acgcgcggtt gccaaaccgt
     4321 tctttaacgt accctcttgc cgtctgaaat ttatctcttc cacccaaaaa ctggcaaggg
     4381 tcaaactgtc cgacgatgca cgcatcctgg cttggggcaa cggcaaagag gccatcgtcc
     4441 gctttgccga acaacaccac atccccctgc tgcgcatgga agacggcttt atccgctcgg
     4501 tcggactcgg ctccaactta gtgccgccgc tgtcgctcgt taccgacgat atgagcattt
     4561 atttcaatgc cgaaaccccg tcccgtcttg aatacatcct acaaaaccaa aacttcgacg
     4621 atcaagactt tcagacggcc ttgaagctgc aaaaaatgct gaccgaaaac cacatcagta
     4681 aatacaacgt cggcagctca gacttcaccg ccccgtcaac cgacaaaacc gtgatcctcg
     4741 ttcccggcca ggttgaagat gatgcgtcta tccgctacgg ttcgccccaa atctaccgca
     4801 atctggattt gctccgtacc gtacgcgaac gaaaccccaa tgcctatatc atctacaaac
     4861 cgcatcccga tgtagtcagc ggtaaccgca tcggccatat ttcccctgaa gatgctgcac
     4921 gatatgccga ccaaaccgcc gaacaagccg acatcctgac ctgtctccaa tacgcagacg
     4981 aaatacatac catgacttcg ctgaccggtt ttgaagcctt gttgcgcggc aaaaaagtca
     5041 gctgctacgg cctgcctttt tacgcaggct gggggcttac ccaagatctg ctccccatcc
     5101 cgcgccgtag ccgcagactt gagctttggc agctgattgc cggcacgctc atccactatc
     5161 ccgactacat ccaccccgaa acccatcagg ccataaatgc agaaaccgca gcccaaatcc
     5221 tgatacgaca aaaaaatatg caaaaaaaca acaacggatt acatcgcggg tgctttgcca
     5281 aaaaattagg taaaatcaaa caactatatc gatctttcaa ataaatacca tcaaagttaa
     5341 cgatgcgtca taaacttgcc tctattgcgg catcattgcc tttgcatcgt taattctctt
     5401 ggcgtatgct tgaaagttca acctaaaact attacataaa aaacaaaacc acattgcaac
     5461 atgaaacaga ccgtcctcaa aaataacctg caaaacctgc ttgaaagcgc agaaaatatc
     5521 ctgctgcttc aaggccctgt cggcgatttt tttctgcgcc ttgccgactg gctgactgca
     5581 aacggcaaaa ccgtacataa attcaacttt aatgcaggcg acgactattt ttatccgccc
     5641 actcaagcgc ataccgttgt ttttaacgac aactacgatg cctttcctga gtttttgcaa
     5701 gaatacatca ctcaacatca catccaggcc gttgtctgct ttggcgacac acgcccttat
     5761 cacgtcattg caaaacgcat tgcaaacgaa aaccaagcca gtttctgggc gtttgaagaa
     5821 ggctatttcc gcccctacta catcacctta gaaaaagacg gcgtcaacgc attttccccg
     5881 ttgccgcgcc gtgccgactt ttttcttgaa caattcccta agcttgccca gcaagaatat
     5941 aaagcgccaa cgccggtaca cggcggtttt acgcccatgg caaaaaacgc tatccgttac
     6001 tatatcgagt tgttccgcaa tctacgcaaa taccccgact acatccacca ccgcgcaccc
     6061 aatgccggcc attacctcaa accgtggtcg ctctccatcc tcaagcgttt gaactactat
     6121 attgaagaca tccaaatcgc caaacgtgtg gaagcaggca aatacggcaa gttttttatt
     6181 gttcccttgc aggtattcaa cgacagccaa gtccgtatcc attgcgactt tcccagcgtc
     6241 cgcagcttcc tgctccatgt tttgagttca tttgccgagc acgcgcctgc cgataccaac
     6301 atcatcatca agcatcatcc gatggaccgc ggttttatcg actactggcg cgacattaaa
     6361 cgctttatca aagaacaccc cgaactcaaa ggccgcgtga tttatgtcca tgatgtcccc
     6421 ctgcccgttt tcctgcgcca cggtctcggc atggtcacca tcaacagcac cagcggcctg
     6481 tccggactga ttcacaatat gccagttaag gttctcggcc gtgcctatta tgatattccc
     6541 ggcattactg accaaaatac cttggcagaa ttttggaatc atccgacacc gcctgacaaa
     6601 gagctgttcc atgcctaccg aatgtaccac ctcaacgtga cccaaattaa cggcaacttc
     6661 tacagtcagg tcttttttcc taacaaaaac acatccgact cttccacacc aacaacctaa
     6721 cttggcgaaa gaaaagctca ggccgtctga aatatttttc agacggcctg aagcaatcaa
     6781 taccttagct actgccatgt aaataaaaca caaaaacctg catttatcat taacaataaa
     6841 ttacaaaaac agtataatga ccgagctgcc atgagcgcat accgactcaa cctgagccct
     6901 ttgtaacaca caaaatatgg atataccccc aggcaaaaca atataacaag ccaaacatcc
     6961 taaagataag ccggcaaggc aatacactct ataaaactgt gccgagcaaa atttttacaa
     7021 agccctcaac cggtatcgcc gcccatatgc cgcagcatcc gtcttccact ttatatccgc
     7081 ccgcaaacca tgaccgccgc tcctgatatc ctctaccggc aagccgccgc ccttttggaa
     7141 caatccaata ccgcccaagc cctgcccctg ttgcaacagg cggcagagca aggttatgcg
     7201 gaagctgctt tcgtattggg caaccatctg ctgcaaaacg gccaaccgga gcaggcactt
     7261 tcatggttgg aagccgccgc ggcccaacgc catcccaaag cactcttctc cctgctgcaa
     7321 caacgcgaac acaacggcac cccgaccgga cagcttctca acgactatgc ctggctgggt
     7381 gagcaggggc actcagaagc ccaattaatc ctcatgcgtt accacgcgca acgcaacgat
     7441 ccacaatcgc tctactgggc ggaacttgct gccgcccgat atgccgcacc tgcgtattac
     7501 catctggcac gccatcatca acgccaaggc gacgttgaaa cagccatcga acaatacgaa
     7561 aaagcggcag cactcggcgt aactgccgcc tgctggcaac ttggtcaaat ctacttctac
     7621 ggtacaggtg tcagccccaa ccacgcacaa gccgaacact atctcgaacc agccgcacaa
     7681 gccggccaca tcgccgcaca aacgctgctg gctgaccttc ttgccgccca acgcaaacct
     7741 gaagccttgg aatggtatcg tcgtgccgcc gataaggaac aagcggaagc acagtctaag
     7801 ctggcccaat acgccctgac cggcgaactt tccgaacgcg atccgttcca agcggcacga
     7861 tatgccaaag ccgctgccga gaaaaaccat cctgaagccc tgaaaatcat gggcgacctc
     7921 taccgctacg gtctcggtat caaagccgac aaccatatcg cgcaagatta ctaccaccgt
     7981 gccgccgcgc tgggttctgc cgccgcagca caaaaactca tcagcgacgc cgcgctgtac
     8041 catccgcaac aatacgaaca aatcaaaact gccgccctgc aacaacaaca aaccgaaacc
     8101 atctaccgtt tggcggaagc acaagcctgc gccatcggcc gtcccgccga ctacaatgcc
     8161 gcgcgaaaaa attacatgga agctgccggg ttccaccata aaaacgcagc ggcagcctta
     8221 ggccgcatct accattacgg cctcggtacg gcgcaagatc ctcgggcggc tgcacactgg
     8281 tacgccattg ctgccgaaca aaaccaccct tccgcccaat accacctcgc ctgtttttac
     8341 tatcacgggc aaggtgtcgg ctgtcatgtt ccgaccgcct gctactggct gcaggccgcc
     8401 atcggcaacg gccacacttc ggccgaatca ttaatatccc tattagaaca atggcgacgc
     8461 gaagcacacc atgccatcgg acaaaaggcc gtctgaaaag atttacactc gcattttttg
     8521 acaatcttta actattcccc taatatttgc cagttatttt tcacggacac gccattgttt
     8581 tcatttcttt ctgaaaacac cttgtccgcg catcaatacc atgacactcg gcggataacg
     8641 ccaagcgttg aaacacacta catccggaac aaaaacggat gctcggaaaa atatttctag
     8701 gaggtgaaac aacatggaat gggaattcaa cagttattac acactgattg ccgccacgct
     8761 cgtgttgctg gttggtaaat ttctggttca aaaaatcaaa ttcttacgag acttcaatat
     8821 tcccgagccg gtagccggcg gtttgattgc cgctatcgtc ctgttcgccc tgcacgaggc
     8881 gtacggcgtg agcttcaaat ttgagaaacc gctgcaaaat gcgtttatgc tgattttttt
     8941 cacgtccatc ggcttgagcg cggatttttc ccgtttgaag gcgggcggtt tgccgctggt
     9001 ggtttttacc gcgattgtgg gcggatttat cttggtgcaa aactttgtcg gggtcggact
     9061 ggctacggct ttgggtttgg atccgctcat cggtctgatt accggttcgg tgtcgctgac
     9121 gggcggacac ggtacgtcag gtgcgtgggg acctaatttt gaaacgcaat acggcttggt
     9181 cggcgcaacc ggtttgggta ttgcatcggc tactttcggg ctggtgttcg gcggcctgat
     9241 cggcgggccg gttgcgcgcc gcctgatcaa caaaatgggc cgcaaaccgg ttgaaaacaa
     9301 aaaacaggat caggacgaca acgcggacga cgtgttcgag caggcaaaac gcacccgcct
     9361 gattacggcg gaatctgccg ttgaaacgct tgccatgttt gccgcgtgtt tggcgtttgc
     9421 cgagattatg gacggcttcg acaaagaata tctgttcgac ctgcccaaat tcgtgtggtg
     9481 tctgtttggc ggcgtggtca tccgcaacat cctcactgcc gcattcaagg tcaatatgtt
     9541 cgaccgcgcc atcgatgtgt tcggcaatgc ttcgctttcg cttttcttgg caatggcgtt
     9601 gctgaatttg aaactgtggg agctgaccgg tttggcgggg cctgtaaccg tgattcttgc
     9661 cgtacaaacc gtggtgatgg ttttgtacgc gacttttgtt acctatgtct ttatggggcg
     9721 cgactatgat gcggcagtat tggctgccgg ccattgcggt ttcggcttgg gtgcaacgcc
     9781 gacggcggtg gcaaatatgc agtccgtcac gcatactttc ggcgcgtcgc ataaggcgtt
     9841 tttgattgtg cctatggtcg gcgcgttctt cgtcgatttg attaatgccg cgattctcac
     9901 cggttttgtg aatttcttta aaggctgatt ttccgccttt ccgacaaagc acctgcaagg
     9961 tttaccgcct gcaggtgctt ttgctatgat agccgctatc ggtctgcacc gtttggaagg
    10021 aacatcatgt atcggaaact cattgcgctg ccgtttgccc tgctgcttgc cgcttgcggc
    10081 agggaagaac cgcccaaggc attggaatgc gccaaccccg ccgtgttgca aggcatacgc
    10141 ggcaatattc aggaaacgct cacgcaggaa gcgcgttctt tcgcgcgcga agacggcagg
    10201 cagtttgtcg atgccgacaa aattatcgcc gccgcctacg gtttggcgtt ttctttggaa
    10261 cacgcttcgg aaacgcagga aggcgggcgc acgttctgta tcgccgattt gaacattacc
    10321 gtgccgtctg aaacgcttgc cgatgccaag gcaaacagcc ccctgttgta cggggaaact
    10381 gctttgtcgg atattgtgcg gcagaagacg ggcggcaatg tcgagtttaa agacggcgta
    10441 ttgacggcag ccgtccgctt cctgcccgtc aaagacggtc agacggcatt tgtcgacaac
    10501 acggtcggta tggcggcgca aacgctgtct gccgcgctgc tgccttacgg cgtgaagagc
    10561 atcgtgatga tagacggcaa ggcggtgaaa aaagaagacg cggtcaggat tttgagcgga
    10621 aaagcccgtg aagaagaacc gtccaaaccc acgcccgaag acattttgga acacaatgcc
    10681 gccggcggcg atgcgggcgt accccaagcc gcagaaggcg cgcccgaacc ggaaatcctg
    10741 catcctgacg acggcgagcg tgccgatacc gttactgtat cacggggcga agtggaagag
    10801 gcgcgcgtac aaaaccagcg tgcggaatcc gaaattacca aactttgggg aggactcgat
    10861 accgacgtgc aaaaagagtt ggtcggcgaa caacgcaagt gggcgcagga aaaaatcagc
    10921 aactgccgac aagccgccgc gcaggcagac cggcaggaat acgccgaata cctcaagctg
    10981 caatgcgaca cgcggatgac gcgcgaacgg atacagtatc ttcgcggcta ttccatcgat
    11041 taggggcaaa ccgatgacta ccgtccccaa aagcaggatt cccgtcaaac cgctgcccga
    11101 aaaaaccaca gacgaagcca aagtcgaaaa atggcggcag ctcggtgcgg aacacggttt
    11161 gtcgggcgaa tgggcagttg ccgccagatt gggcgagaac ggttttaccg aagaacagat
    11221 ggaaaatatc gccaacctgt tcggcagata aagggaaaat tgacggaaat gccgtctgaa
    11281 accctgttat cggtttcaga cggcattttg actaatacgg tacgcaggcg caaaacagtc
    11341 ggcttttcct gcgtttccta tgctgatgtt tcaacacaca ggacgataca aaaagcgtcg
    11401 ccctatgtgc cgtcctgatt cggaaagggt tacgcccctc c
//
LOCUS       DAXHKX010000045        11213 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHKX010000045 DAXHKX010000000
VERSION     DAXHKX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.045"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            107..1060
                     /locus_tag="ACFMWZ_000979"
     CDS             107..1060
                     /locus_tag="ACFMWZ_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC5934957.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1171..1494)
                     /locus_tag="ACFMWZ_000980"
     CDS             complement(1171..1494)
                     /locus_tag="ACFMWZ_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934958.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1667..3028)
                     /locus_tag="ACFMWZ_000981"
     CDS             complement(1667..3028)
                     /locus_tag="ACFMWZ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC5934959.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3383..5083)
                     /gene="recJ"
                     /locus_tag="ACFMWZ_000982"
     CDS             complement(3383..5083)
                     /gene="recJ"
                     /locus_tag="ACFMWZ_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC5934960.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5152..5868)
                     /locus_tag="ACFMWZ_000983"
     CDS             complement(5152..5868)
                     /locus_tag="ACFMWZ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934961.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLEFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAV
                     GAVLALPVAALIAATNSSDK"
     gene            complement(5915..6427)
                     /gene="yjgA"
                     /locus_tag="ACFMWZ_000984"
     CDS             complement(5915..6427)
                     /gene="yjgA"
                     /locus_tag="ACFMWZ_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC5934962.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MESQGGTGEA"
     gene            6581..7912
                     /gene="pmbA"
                     /locus_tag="ACFMWZ_000985"
     CDS             6581..7912
                     /gene="pmbA"
                     /locus_tag="ACFMWZ_000985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC5934963.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEQALQDTVKAAIDIARYTAEDDCA
                     GLADASLMAQHVGDLDRYHEWDLSTEAAVELAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTISGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8209..8412)
                     /locus_tag="ACFMWZ_000986"
     CDS             complement(8209..8412)
                     /locus_tag="ACFMWZ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC5934964.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8655..8957
                     /gene="clpS"
                     /locus_tag="ACFMWZ_000987"
     CDS             8655..8957
                     /gene="clpS"
                     /locus_tag="ACFMWZ_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC5934965.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8959..>11213
                     /gene="clpA"
                     /locus_tag="ACFMWZ_000988"
     CDS             8959..>11213
                     /gene="clpA"
                     /locus_tag="ACFMWZ_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC5934966.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNSDIPDALKDTEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPQAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTEVAKQ
                     LAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPVIAKVVDKFLLQLEHQLLDKKVEAE
                     FTPALRKYLAEKGFDPQMGARPMNRLIQEKIRKPLADELLFGKLAEGGFVRIDWDAAK
                     EEAVLKFKKSKV"
BASE COUNT         2672 a         3251 c         2835 g         2455 t
ORIGIN      
        1 ttagagaatc gttctctttg agctaaggcg aggcaacgct gtactggttt aaatctaatc
       61 cactatagat taaaatcaca caatatccaa ctattcggac cttcatatga cacataccgt
      121 ccacctgcat ttagaagaaa ccgacaactt ggcgttgcag cgtctgtgcg gttcttttga
      181 caacaacctt gatttacttg ctaaagcact cgatatccac atcagccgcc gttttgaaca
      241 tttcactttc aacggcgcat ttgcacacgc cggcaaacgc gcactgctca aactcttgga
      301 aacggcgcag acgcgcgacc taaacgacgg cgacatcagg cttgccgccg tcgaagccca
      361 aaccgaagat gccggtcatc aagaaaaaaa ccatgaccac gcctattatt tccgcaccaa
      421 gcgcggcagc atcggcggca gaacgccacg gcagaacggc tatatccgcg ccctactcaa
      481 ccacgacatc gtattcggtc tcgggccggc aggtacgggc aaaacctatc tcgccgtcgc
      541 cgccgccgtc gatgcgatgg aaaaacacca agtcgaacgc atcattttag tgcggcctgc
      601 cgtcgaagcc ggcgagaaac tgggttttct gcccggtgac ctgacccaga aagtcgatcc
      661 ctaccttcgt ccgctttatg atgccctcta tgacctgatg ggctttgacc gtgtaaccaa
      721 gctgattgaa aaaggcctga ttgaaatcgc cccgctcgcc tatatgcgcg gcaggacgct
      781 caacggcgca tacatcatcc tcgacgaagc gcaaaacacc acgcccgaac aaatgaaaat
      841 gttcctgacc cgcatcggct tcggcgcgaa agccgtcatt accggcgaca ccagccaaat
      901 cgacctgccc aaaaacatca aatcgggatt aaaagatgcg cgtgagaaac tgcacaacgt
      961 ggaagggctg tatttccaca cctttaccgg cgaagacgtt gtccgacatc ctctggtgca
     1021 aaaaatcgtc gaagcctacg aatcggcaga acacgactga cattccaatg ccgtctgaaa
     1081 cccgatgcat ggaaaatcgg gcagagaaaa acagatacgg aagccaagct ccgtatccgt
     1141 atccgtttct ttatccgtcc aaccgataca tcaatcgaat tttctgcatc ggattgcctc
     1201 atcttcgcaa aacattgccg aagtttcgcg gttgcacgcc tgacgcactt tctgccgcgc
     1261 cacgccttca ttccgatcgc ttgcctcaaa cgtcttctga aaaggcgtaa cgctgcatac
     1321 atacatggtg gaatttgaac cgaaaaggtc gattttgacg gtattgctgc cgctttccaa
     1381 tacggcgaca cggtgttcca actgcgccag acgtgcttcc aaatcgccga ttctgtcggc
     1441 aaatgcaggc atggcggcac acgcggtcaa aatgcccaaa atacattttt tcatcaattt
     1501 ttccttgttt cttgaaccct gcccacccgg acatcctttt tccgagaggc agggttagac
     1561 tttatttacg gggatgaatc cccttacaaa tcaggataat acatagaacg gcactttatg
     1621 cttcatccca tgtgttgaaa cttcattgct ccttgtctgt tgacccttat tcccaatccg
     1681 ttcccacaac cttcggcttg ggtttgcgcc tgcgacggcg gcgttttttc gcctgtcctt
     1741 cgtttttttc gtgtcgggcg gcggcttcgt ttttggtcat ctccgaccgc tgttccgtcg
     1801 atgccgtctg aaacgccgtc caccactcgg caagggcgcg gtccgcattg ccggtttcgg
     1861 cgcgcaagag caggaaatca taggcggcac ggaaacgcgc ctgtgcaaac agtttgtgcg
     1921 gacgtgcgcc tttgcgattt tcaaactgcg gctggaacat ccaaatttcg cgcatcgtgg
     1981 cggaaaagcg ttgcggcacg ccccaaccgc gttcgacggt ttcgcgcatc gtattgattg
     2041 catcggacag ggcgggcacg ggtttcaaac cctgttgcag attgcttttc caatggcgtt
     2101 ccaactcggg ccacatcagg gccgccagta cgaaaccgac cgaaaccgat ttgtcggcac
     2161 gcagccgctc atcggtattt ttcagggcaa gcaccgtcat ttttccggcg atgccgtctg
     2221 aaacgcgcaa ggcattgagc agcggatgga tgtcgtccgg tatgtcaaat ccgttcaaac
     2281 gtttcagaca ctcgcgcgcg tgccctgaaa acagcagttt cataatttcg tcgaacagcc
     2341 gcgctaccgg ttcgtgcttc agacggcata tcgattcggc aatcggtgcg gcggtttctt
     2401 ccgacagatc aaagcccaat ttgctcgaca ggcggatggc gcgcaaaatc ctgacagggt
     2461 cttcctgata gcgttcggcg gcatcaccaa tcataaccag cctgcgggcg gcaacatcgg
     2521 caatcccgtt gtggaaatcc aaaatctctt ctttttcagg atcgtaatac aaggcattgc
     2581 aggtaaaatc gcgccgcatc gcatcttctt cgatgctgcc gtaggtgttg tccttcatga
     2641 ttctgccgtg cgcgttctga tgaacctttg caccgccgcg aaacgtcgtt acttcgataa
     2701 tctccgcacc gttcatcaca tggacaatct gaaaacgcct gccgatgatg cggctgcggc
     2761 ggaagagttt gtgcacctgt tcgggcgtgg catcggttgc cacatcgaaa tccttgggtt
     2821 cgatgccgag cagcaggtcc ctgaccgcgc cgccgaccac ataagcctga aaccccgccc
     2881 ctttcaggcg gcgtatgacg ttttcggcgg caaagctcaa catttcggca cggatgttgt
     2941 gtctttcggc aggaatgacc gttttacttt ccgctttttt actgctccga ccggaaggca
     3001 gcatcttatt caaccatttt ttcagcataa atattctgac ccgaagagcg gcatctccac
     3061 gggcaaccgt gttcagacgg catcgggcgc aggtttgcaa gatgccgtaa ccataaacaa
     3121 aaggcgtaga aactacgccg tccgaatcac gccgccctcg cggcggcaac gcgtcattat
     3181 aacagattgc cctccaaaaa aatcagaccc tgcttttgtg gcggagtctg aaatttgatg
     3241 caccttatat attgcgtccc taagaaagga tgattaacaa aaattaacgc cctttacttt
     3301 cttttccgat tgggaacatt cccgtcgtac aaacaggcaa atgaaggaac agaaatcaca
     3361 ttcaaacggc gttccgccgc ctctacgccg cttcccagta gtcgatataa agctgcaatt
     3421 cgaggttgtt gcgccattcg ttggcaacgg ggcggtacac cgtgcggatg tattcgggga
     3481 tgtcttcgct gcaacgccaa aacatcgctt caaattcgca gccgtctttt tgcagccaga
     3541 cttttttgtg tttgccctcc gcgcccaaag gttgctggcg gacgacgtgg aactcgtcgg
     3601 taaaactcgg cggcgcgaag ccctgccccc aaacgtgttt agcaaggttt tgcgcctgtt
     3661 ccagcgtaat gtcgcgtgca ggcaggctgc cgtcggtgat gaaggtttgc gacaaatcgt
     3721 cttcgcacac catttcgcgc acggcttctt caaaggccgt ctgaaacgcg ggaatgttgt
     3781 gttcgagtat gctcaagccc gccgccatcg cgtgtccgcc gaatttcaaa atcaaatcgg
     3841 gatggcgttt ggacaccaag tccaaagcat cgcgcaagtg aagattggga atggaacgcc
     3901 ccgaaccgcg cacttcaccg ttgtcggcag gcgcaaacac gatggtcgga cgataaaaac
     3961 ggtctttgag gcggctggca acgatgccga ccacgccttg atggaagtcg tcgcgatacg
     4021 ccaccaaagt catctgacct gaaggcaggg tttcaggaaa agcattcagc gcgtcttgca
     4081 gcatagactg ctcgatttcg cgacgttcga tattgaggtt gtttaactgc gccgccagtt
     4141 cctgcgcctc ggtatcatct cgcgccaaca ggcaggcaat accgaccgac atatcgtcca
     4201 gccgtccggc ggcgttgatg cgcgggccca acgcaaaacc catatcaaac ggctgcgcct
     4261 tgcgccaatc gcgccgcgcc acttcaaaca aggcgcggat accggggcgc attttgccgg
     4321 agcgcatccg tttcaaacct tgcgacacga ggatgcggtt gttgtgatcg agggagacga
     4381 cgtcggcaac ggtaccgagt gcgaccaaat ccaaaaggtc gcccagattc ggctctttta
     4441 ggccgtctga aaaataattg cggcggcgca gttcggcacg caaagccatc agtacataaa
     4501 aaatcacgcc cacgcccgcc aagcttttgc tttggaaacc gcagcctttt tggttcggat
     4561 tgacgatgat gcagtctggc acggtctcgg caggcagatg gtggtcggtt acaatcacat
     4621 ccaaacccaa ttcctgcgca cgcgccacgc ctgcgatgct ggcaatgccg ttatcaaccg
     4681 taatcagcaa atccacgcct tgcgcggcgg cgatttcggc cagctcgggc gttaaaccgt
     4741 agccgtgttc aaagcggttg ggcacgagga aatctacttt tgcccccatc gccgccaaac
     4801 cgtccaaacc gacggcacac gcggtcgcgc cgtcggcatc gtagtcggcg acaatcaaga
     4861 ttttttcctg acgctcaacc gcatccgcca aacggcgggc ggcggcttcg caattcgtca
     4921 gcgtttgata aggcaggagg gaagcgagtt tgtcgtccaa ttcggcagga ctttgcacac
     4981 cgcgcgaggc acaaagccgg gcgattaaag gatcggcgcc ggcggtgagc aaatgattga
     5041 aaacgtcggt attgacggat cgggtttgga ttttgacgga catagcgtgg aactttacag
     5101 tgcggatgtg tttgaaatgc cgtctgaagg cttttcagac ggcattgccg ttcatttgtc
     5161 tgaggaattc gtggctgcaa tcaacgccgc gacaggcaag gccagcaccg cgcccaccgc
     5221 atccagtatc aacgtggtgg gcgtatatgc aatattttca aacaacttgg atttgtcggt
     5281 atgttttttc gtaaccgtgt aataaatatc ggcaggcaca ctttgctcaa aatgataatc
     5341 ggcgttcagt ttttgcggtg tggcgtagta tttgcctttg gcggagacgc agcgcgtgta
     5401 aatggtccga ttgtcgagtt cgaccgcttc aaactcaagc tgtttcagct tggcgatgtc
     5461 ggcaggcttg tcggtatcgt agcgcaggca aaggccttcg gtactgaaat tctggctgcc
     5521 aggcgattcg agtttgaccg gcagggcttg gtggcgagca tagctcgggg tatcctcaac
     5581 tatttggaag ggtttgtcca gccctgcctt caaaatgccc gtcagcttcg ccgaatcttc
     5641 gggattgacg acgaaccagt atttcccgcc catcatcacc agactgccct tttccaattg
     5701 ggcattgtct ttggcaacca caccgaaggc gcggatttga tccttgtcaa cgtgtttata
     5761 ggcggtcgtt tggctgaacg ggtcgttcat gccccacatc attgccgtac agccgttcaa
     5821 catcaaggct gccgccaaaa cggcggcggt cgttttacgg aacaacattt tatcttccaa
     5881 tcagaatgac ggcggatgcc gtctgaaaat atgcctaggc ttcccctgtt ccgccttggc
     5941 tttccataac ggttttcaac tcttgaaaca gggcgcggaa atttttcggc ggtttgttct
     6001 gttcctgttc ttttttggta ttgcggatga gcgtcctcag cttgcccgca tcggcgtttg
     6061 gaaaatcgga cataaactgc gtcagcgcgc cgtcgtccgc cagcagctcc atccgcgtcc
     6121 gctccacacg ttgcaaaaag gcgttgtgcg ccgcatcgtc gccgcgcagc ttggcaagaa
     6181 atgcctcgat gggcgcggga tcggtatcgc gcatcagtct gccgataaat tgcgcctgcc
     6241 gcttgagcgc gccgttggat gtgatttttt tataggtaac gatggcttcg tacaaatctt
     6301 cgtccaagcc gatttttttc aacgtgtcgc ttgagagctt ggtcagctcc atacccaaat
     6361 cctgcaaacc gttcatctgc tttttcattt gggttttgct gacccattcg tcttcttgtt
     6421 caaacatctt cattctcaaa ttttatccga cgggcgcatt ttaacagaat gattccgcgt
     6481 tcagacggca ttttgcaccg tccgccctgc aaacggacgc ttttgcccct gccgcccgaa
     6541 acggttaaaa tgccgcaaag catcatcaag aaagatttct atgctgttca accacaccgc
     6601 ttccgaactc ctcgacctct gccgccgcac gctcgactta gcaaaagcga cgggcgcaac
     6661 cgccgccgaa gccgatttca gcgaatcatt gggacaaagc gtcagcgtgc gcttgggcga
     6721 aatcgaacaa attgagttcc agcaggacaa gtcgctggac attaccgttt acgtcggcaa
     6781 acgcaaaggc cgcgccagca ctgccgactt ctccgaacaa gccctgcaag acaccgtcaa
     6841 agccgccatc gacatcgccc gctacactgc cgaagacgat tgcgcaggtt tggcagacgc
     6901 ttcgcttatg gcgcaacacg tcggcgacct cgaccgttac cacgaatggg atttaagcac
     6961 ggaagccgcc gtcgagttgg caaaacaatg tgaacaagct gccctgaacg aggatgagcg
     7021 catcgaaaac tccgaagggg cggcggtgca aaccggccat taccaatacg tttacggcaa
     7081 cacccacggt tttgccgcac accggcaagg cacgcaccac agcatttcct gcagcgtcgt
     7141 tgccgccgac gaaaacggga tgcagcgcga ctactggtac gattccgcct gccgccatcc
     7201 ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt tgcgccgact
     7261 cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca ccatttcggg
     7321 cggtcttatc ggacacctcg tcggcgcact ctccggcggc gcgctctacc gccaaagcag
     7381 tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc tgcgcgaaga
     7441 accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggcg ttgccaccgc
     7501 accgcgcttc gtgattcaaa acggcattgt cgaaggctat ttcctcagca gttacagcgc
     7561 gcgcaaactc ggtatgcaga ccacgggcaa cgccggcggc gcgcacaacc tgtatttgaa
     7621 ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg gggacgggat tgttggtaac
     7681 cgaactgatg ggacagggcg cgaacaccat taccggtgac tactcgcgcg gcgcggcggg
     7741 tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg tagccggacg
     7801 cttgcaggat atgtaccgcg acattgtcgg cgtggcggat gacgctttgc ggcgttcgtc
     7861 caacaaaatc ggctcgattc tgattgcggg gatgacggtt gccggaagct gaacgccctg
     7921 ccgatgccgt ctgaagacga aggcagcacg ttttgccccg tgcctacccg tttgccgttt
     7981 tatgccgcac cgccttttgc cgacaacatt gcacattttt tcatcagcac tgattttccc
     8041 ttatccgtca aaatactaag ccacttgtcc acaacagggt acacacaagt tcaggataag
     8101 gcgtgcgggg attggggttt ttccgccgcc ggcaaaacgg cagacaaaaa cggcgggaca
     8161 ttgcctgtcc cgccgttttc aaatatacat ttgaccgtac atcaggaatt aagcagcctg
     8221 aatgttggcg gcctgtttgc ctttagggcc ggtggttacg tcgaaagaga cgcgttggcc
     8281 ttctttcagg gttttgaaac cttccatatt gattgctgag aagtgagcga acaaatcttc
     8341 gccgccttca tcaggagtga tgaaaccaaa acctttagcg tcgttaaacc attttacgat
     8401 accggttgcc attagaaact tcctatactc aaaaattaac aaaatcagca aaacaaggca
     8461 tacggcaaac cgagccgtct agcgttcctt ccagcttgat atactcggcg gatgcccaat
     8521 cctgcttaac cgtcttttta catttgttaa gacaaaacgt caagctatgt tttcaaaaaa
     8581 gtgggaaaat aggcaaaagt accgaaaata caacaaaaat gccgtctgaa cgcggctttc
     8641 aaatgggaca cactatgacc gcgcaacacc aatccgacac gctcttgcac cgtctgaaca
     8701 ccctgccgcc gaaacgttac ggcgttttcc tattgaacga cgattacacc acgatggaat
     8761 ttgtcgttga aatcctgacc gaaatcttca tgctcggaca agaacaggcg gtagcggtaa
     8821 tgctcttggt tcatcacgaa ggcaaaggcc tgtgcggcac ttacacgcgc gatattgccc
     8881 aaaccaaaca gcaacaagtc atgcagcggg caaaagccga agggcatccg ctgcaatgta
     8941 ttgtcgagga gatttaatat gcttgcaccc gaattggaac agattttgca gcagctttac
     9001 cgcgaggcgc gtaaggctca ttatgaattt atcagcctcg agcatctgct tttggtactc
     9061 atcgaagaag atgcctccgt gccgaacgtc ttaaaactat gcggcgcgga tttgaaagtg
     9121 gtgtccgaac agctcgccgc cagcgttgcc gaaaacaccc ccctgattcc cgaacacctt
     9181 ttagacacgg tcgaaacacg gcccacgctc ggcttccaac gcgtgatgca acgggcgatg
     9241 gtgcataccc agtctgccgg aaaagccgca gtcgaaccgt tggacgtttt ggtcgcgctg
     9301 atgagcgaaa ccgacagcca caccgtctat ttcctcaagc tgcaatcggt tacgcgtttt
     9361 gaagttttgc gctgtattgc ccacggctcg cccgatgacg aggatggcaa tgattcagac
     9421 ggcctgggac gcgagggcga agaagcggaa caaaaaaccg gctccctttc cgactacacc
     9481 gtcaacctca acgccgaagt caaagccggc cgtatcgacc ctttgattgg tcgcaaacac
     9541 gaaatggaac ggctggtgca aatcctgtgc cgccgccgca aaaacaatcc gcttttggtc
     9601 ggcgaagcag gtgtgggcaa aaccgcgctg gcggaaggtt tggcacatca aatcgtcaac
     9661 agcgacattc ccgacgcgct caaagatacc gaagtgtacg cgctggatat gggttcgctg
     9721 ttggcgggca cgaaataccg cggcgacttt gaagcgcggg tcaaatccgt cttgaaacag
     9781 ctcgaaaaaa tcccgcaagc cattttgttt atcgacgaaa tccacaccat catcggcgcg
     9841 ggcagcacca gcggcggcac catggacgcg tccaacctgc tcaaacccgc gctggcaaaa
     9901 ggttcgctgc gctgcatcgg cgcaaccacc tacgacgaat accgcaccat tttcgacaaa
     9961 gaccatgcct taagccgccg cttccaaaaa atcgacgtgg tcgaacccac cgtttccgaa
    10021 accgtacaaa tcctgcgcgg cttgaagccg atgtttgaag ccttccacca agtccgctat
    10081 acgcaaggcg cactcgaagc cgccgccgaa ctctccgcac gctacatcaa cgagcgtttc
    10141 ctgcccgaca aagccatcga cgtgatggac gaagcaggcg cggcgcaacg gattctgccc
    10201 aaatccaaac agaaaaaagt catcggcaaa gcgcaaatcg aaaccgtcat cgccaaagtc
    10261 gcgcggattc cagaaaaaac cgtgtcgcac gacgacaaac aggtgctgca attcctcggc
    10321 cgcgatttga aaaacatggt ttacggtcaa gaaaacgcca tcgacgcgct ggttgccgcc
    10381 gtcaaaatgt cgcgttcggg cttgggactg cccgacaaac cgataggcag cttcctcttc
    10441 tccggtccga ccggcgtcgg taaaaccgaa gtcgccaaac aacttgccta ctcgatgggc
    10501 gtaccgctgc aacgctttga tatgtccgaa tacatggaac gccatgccgt atcgcgcctc
    10561 atcggcgcgc ctccgggcta tgtcggcttt gaacaaggcg gccttttaac cgaagccgtc
    10621 aacaaacagc cgcactgcgt attgctcttg gacgaaatcg aaaaagccca ccccgacatt
    10681 ttcaacgtcc tcctgcaagt catggacgca ggcaaactga ccgacaacaa cggcaagagt
    10741 gccgatttcc gcaacgtcat cctgattatg accaccaacg caggcgcgga aagcctcagc
    10801 cgccccggcc taggctttac cgctaagcgc gaacgcggcg acgaaatgca ggccatcaac
    10861 aagctcttca cgcccgaatt ccgcaaccgc ttggatgcga ttatcccgtt tgcacccctg
    10921 tccgaacccg tcatcgccaa agtcgtggac aaattcctgc tccagctcga acaccagctt
    10981 ctcgacaaaa aagtcgaagc cgaattcacg cccgcattgc gcaaatatct ggcggaaaaa
    11041 ggtttcgacc cgcaaatggg cgcgcgccca atgaaccgcc tgattcagga aaaaatccgc
    11101 aaaccactcg ccgacgaact cctgttcggc aaactcgccg aaggcggctt tgtgcggata
    11161 gactgggatg cggcaaaaga agaagccgtg ttgaaattta agaaaagcaa ggt
//
LOCUS       DAXHKX010000046        11140 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHKX010000046 DAXHKX010000000
VERSION     DAXHKX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.046"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="ACFMWZ_000989"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="ACFMWZ_000989"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC5934967.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEILLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSCQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1612..2487)
                     /locus_tag="ACFMWZ_000990"
     CDS             complement(1612..2487)
                     /locus_tag="ACFMWZ_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC5934968.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVEATGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHGGGKKSGSAWRQAVLLLGVWAGYGI
                     IDILFKQLAKSGTAFSGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2489..3211)
                     /locus_tag="ACFMWZ_000991"
     CDS             complement(2489..3211)
                     /locus_tag="ACFMWZ_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC5934969.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKTEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="ACFMWZ_000992"
     CDS             complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="ACFMWZ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC5934970.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFSRLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKRIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3788..4150
                     /locus_tag="ACFMWZ_000993"
     CDS             3788..4150
                     /locus_tag="ACFMWZ_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC5934971.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATA"
     gene            4185..5048
                     /locus_tag="ACFMWZ_000994"
     CDS             4185..5048
                     /locus_tag="ACFMWZ_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC5934972.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPKNTLPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5146..6105
                     /gene="ttcA"
                     /locus_tag="ACFMWZ_000995"
     CDS             5146..6105
                     /gene="ttcA"
                     /locus_tag="ACFMWZ_000995"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC5934973.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6199..9138)
                     /locus_tag="ACFMWZ_000996"
     CDS             complement(6199..9138)
                     /locus_tag="ACFMWZ_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC5934974.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTEVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYRIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9151..>11140)
                     /locus_tag="ACFMWZ_000997"
     CDS             complement(9151..>11140)
                     /locus_tag="ACFMWZ_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC5934975.1"
                     /translation="DLSDTAGANTEAIYTSDGITANSTQLEQLKKLFPACFDADGNFL
                     IDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQN
                     LLIRGDNLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELALLANID
                     EDEAARILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLC
                     DEVFGEGNFVAQLPWRKRTAKSDVPFGISQDYEWIFVFAKSCQFIAATKGKERRYYET
                     DDFPDRPWRTHDLTKQTTAAERPNSFFTMVDPKTGKKYPANPNATWRVTKDTFQDYYN
                     KGKIVFPDDYDFLNISNPVMRYFKDDDMKKAGEDFGKVAVSSRLPENVGTLADAVAEY
                     LAIFSRTLPENIGMTKEGTKEITDLFGSKIFTFPKPSQLIKFLVSISSKSNDLILDFF
                     AGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARKAGYPTIFDITKARIEKA
                     AAKIRAEHPDYTGDLGFKIFQTADNFRQHPDNDFSPEQPDLPLSDELSEEQLQTLLTT
                     WTLYDGAALTTPVEPVRLGSYTAYLCEKRLYLMNAGFTSADLLAFIRKLDDDAAFNPN
                     RVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
BASE COUNT         2664 a         2975 c         2659 g         2842 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgct gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccgaagtgt attggggcat ccacaccctg cgcgccattg aaaactttaa
      241 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgccgccgc
      361 cattgaaaaa gcctgcgacg aaattctgct gaacaaccgc tgcctcgacc aattcccgtc
      421 cgacgtgtat cagggcggtg cgggtacttc ggtcaatatg aacaccaacg aagtcatcgc
      481 caacctcgca ttggaagcct tgggctatga gaaaggccgc tacgacatcg tcaatccgat
      541 ggaccatgtc aacgccagcc aatcgaccaa cgatgcctat cccacgggct tccgccttgc
      601 cgtgtattac agcatcggcg aattgctcga caaactgact gtcctgaaaa acgcctttgc
      661 cgccaaggcc gaagaattta aagatgtttt gaaaatgggt cgcacccagc ttcaagatgc
      721 cgtgccgatg actgccggac aagaattcca atcttgccaa gtgttgttgg aagaagaaat
      781 cctcaacctc gaccgcacgc gccaactgct tttagaagtc aatttgggcg caacggcaat
      841 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      901 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgacacg acgattacct tcgccgccga
     1201 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 catttccctc ttgggcaatg ccgcagtcaa cctgtccgac aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatctgcg aacgttacgt tttcaactcc atcggtttgg tgacttatct
     1381 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1441 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1501 catcctctct cccgagaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1561 tgaagcaggg atgctgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1621 gaacagtgcc ttgacctccg cccaataaaa caggcaggcg atggaacaca gtgccaacac
     1681 gattcccgcc gtattgattg tgttgatttt ttccttaaaa aacagtgcgc ccgaaagcgt
     1741 ccccaaaaca atcacgccga tattcatacc ggcaaaaacc agcgtgggat tgtccttcat
     1801 catttggtgc gcggtgatgt aggttacgat gttcatgaag ttcaaaccgc ccaagagtat
     1861 gccgccgacc acactctcaa cgcgccatct gaccgatttg gcaaacaggc aggcaaacat
     1921 cagcacacct gccagcacaa atgcaaccag caggttgcct gaaaatgccg tgccgctttt
     1981 ggcaagctgt ttgaacagga tatcgataat gccgtaacct gcccacacgc ccagcagcaa
     2041 taccgcctgc cgccacgcgc ttcctgattt tttgccgccg ccgtgcttcc aaagcaggca
     2101 gaacagtgcg gcaaatgcga ggcacagtcc gattagtttg ccttcgctga gtttttcgcc
     2161 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2221 tttgacgata ccggtggctt ctacggattt gcccataatg acaaatacgg acggcagcag
     2281 cacgcccaat gcggcaaaca gccaccacgt cggcaaaaat gcgccgatat tgccgacatc
     2341 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2401 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg atacgctgaa
     2461 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2521 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2581 taataatcag cacgccgata cccaaaaggg cagtcagcgc gcccaaaata taaagccaaa
     2641 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2701 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatatag gcgaaaatca
     2761 cgccgactat gggcatgaac agcatgattg cacccaaacc gaacaggatg taggcggcga
     2821 ggacatagtt gcgcccgctt ccgccgccgg acggttcgta agtatggcac gaacgtgatg
     2881 cctgccgttc aaacgcctgt ctgaacgcct gttcgcggcg catctgttcg cggtaaaact
     2941 gctccctgcg cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcatgcc
     3001 gtctgaagga cgcgtcgtat tgcatccgtt tcgacaggtc ggaaagcgta tcgtaagccc
     3061 gttggatttc tttgaacttt tcttccgtct tcggattgcc cggattgcgg tcgggatgat
     3121 atttcatcgc cagcttgcgg taggcgcgtt tgatttcgtc cgctcccgcc tgcggcgaca
     3181 cgcccaatac ggcatacaaa tctttgtcca tattgttcct ttatactttc ccgtcaaacc
     3241 gccgcctgta ttgttttcgg gttcaaaata tttaaatctc cgggacggat gccgataatg
     3301 aacccgcgtt ttccgccgtt gatatagatg gtttccaaat ccatcaccga ctgttcgacg
     3361 taaatatcca attttgtccg gatgccgaac ggcgttgtgc cgccgaccaa atagcccgtc
     3421 cacttgtttg cctgtgcggg cgtagcgggt tcgatgcgtt tcgcacccaa atgccgagcc
     3481 agattgcgcg tggaaatctg tttgtcgccg tgcatcaaga caatcagccc cttaccgttt
     3541 tcatcttgca aaacaatggt tttaatgacc aagtgttcgt ctttgccgaa cagtcgggaa
     3601 aactgcgccg tgccgccgtg ttcctcatag gcataggtaa aaggttcaaa ttcgatgccg
     3661 ttttcacgca aaacgcgcac ggcaggtgtt atcggataag tgtgtttact catttttcgt
     3721 catgcaaaac atggaaatcg gtcaagcgca atgttaccat atatcccctt tatcgaagga
     3781 aaccgatatg gatatccgat atttcggcac aacgccccgc tattccgaag ccgtcggcgc
     3841 aaacggcttg attttcctct ccggcatggt tcccgaaaac ggtgaaacgg ctgccgaaca
     3901 gactgccgac gtgcttgccc aaatcgaccg ctggctggcg gaatgcggct cggacaaagc
     3961 gcacgttttg gatgcggtca tctatctgcg cgatatgggc gactatgcgg aaatgaacgg
     4021 cgtgtgggac gcttgggttg ccgccggcag aacgcccgcc cgcgcctgcg tggaagcgcg
     4081 gctcgcccgt ccggagtggc gcgtcgaaat caaaataacc gccgtgaaga aagatgcggc
     4141 aaccgcataa acagatttcc tttatatttc agagtacgca aatgatgaaa accttactgc
     4201 cctttttcgg cgtggtacgc gtcagcggcg aagaccgcca aaccttcctg cacggacagc
     4261 tttccaacga catcaataat cttcagaccg ggcaggcgtg ttatgcgact tacaacacgc
     4321 ccaaaggccg tgtcatcgcc aatatgattg tcgtcaaccg aggcggcgat ttgctgctga
     4381 ttatggcgca agacttgctt gaagcaacgg tcaaacgttt gcggatgttt gtgttgcgtg
     4441 ccaaagccgt ttttgaaatc cttgaagatt acgccgtcgg cgcggaattg gaagcatccg
     4501 ccgaaccgct tgccgcgcaa gaacccaacc ttgcctttgc cgcacaacaa gattcagacg
     4561 gcatctgctc tatcgctctg cctcatggcg gcatccttcg catcgccccc aaaaacacct
     4621 tgccgcctta tgatgccgcc gccgaaaacg cgtggcggct gcacgaaatc cgtagcggtt
     4681 atccgtggat atgcgcggct accaaagaaa ctgccgttgc ccaaatgctc aaccagcaca
     4741 tcatcggcgg cgtacacttc aaaaaaggct gctatcccgg tcaggaaatc attgcccgtg
     4801 cccagtatcg cggtcaggtc aaacgcggct tggcggtatt gtcgggaaat tcggccgtcg
     4861 aagcaggaac cctgctgacg gcagacggcg aagagaccgg catcgtactc gacagcgttc
     4921 aagattcgga aaactttacc gcccttgccg tgattaaatt ctccgccgcg caaaaagaac
     4981 tgaccgcccc aaacggcagt atcttcaaag cggttcatcc cttctttaaa accgaaaatg
     5041 cagaataatc cctctaatgc cgtctgaacc tttttcagac ggcattttaa aaccgcccct
     5101 gttttcggtt acaatgccgc atccccaatt tttcaaacca ccgccatgtc caaaaaaacc
     5161 aaacaagaac tggaaaacaa caaactcagc aaacgcctgc gccacgccgt cggcgacgcg
     5221 attaacgatt tcaacatgat cgagccgggc gacaaaatca tggtctgcct ctccggcgga
     5281 aaagacagct atgccctctt ggacatcctg cgccagctcc aagccagcgc gccgattgat
     5341 ttccaactgg ttgccgtcaa tctcgaccaa aagcagccgg gcttccccga agaagtatta
     5401 ccgacctatc tcgaaagcat cggcgttccc tacaaaattg ttgaagaaga cacctactcc
     5461 accgtcaaac gcgtattaga cgaaggcaaa acgacttgtt cgctgtgcag ccgcctgcgc
     5521 cgcggcatcc tctaccgcac tgcaaaagaa ttgggctgca ccaaaatcgc cttgggacac
     5581 caccgcgacg acatcctcgc caccttgttt ttaaatatgt tttacggcgg caaactcaaa
     5641 gccatgccgc ccaagctggt gagcgacaac ggcgaacaca tcgtcatccg tccgctggcg
     5701 tatgtaaaag aaaaagactt aatcaaatac gccgaattaa aacaattccc catcatcccg
     5761 tgcaacctct gcggctcgca acccaacctg caacgccaag tcatcggcga tatgctgcgc
     5821 gattgggaca aacgctttcc cggccgcatc gaatcgatgt tctccgccct gcaaaacgtc
     5881 gtcccttccc accttgccga taccgaactc ttcgactttg ccggattgga acgcggtcaa
     5941 accctgaaac acggcggcga tttggcgttt gacagcgaaa aaatgcccga acgtttttca
     6001 gacggcagcg aagaagacga aagcgaaatc aaaatcgcgc cgcaaaaagc cgaacgcaaa
     6061 gtcatcaaca ttctggcgaa caagccgaaa acctgcggcg cgtaaccccc cgccgtcaaa
     6121 aatgccgtct gaagcccgtt tggcgtttca gacggcattt taaaatcccg aagcagacgg
     6181 cttccaaaca tttgtttctc agtgtccgtt ttcagaatgc ccgcctgcgg gcatattttg
     6241 cccgatcagt tcggctatcc tctcgccgtc aaactgcgtt ttgaacacca ccttgatttc
     6301 tttggaaatc tcggcaaaca gcttttcggc atgtttgatt ttccgctctt cgctttttcg
     6361 caaatcttcc gccccgtcag tccctttggc ttcaatcaca aagttcagga tctcgccgct
     6421 tttggttttc acgatatagg caaaatcggg cgaatacgtg ccgccgccgg caacagggat
     6481 tttgatggag tttctcggta ttttggtaaa tacggttacg ccttcaattt ggttgttggc
     6541 gacattttca tgttctatat ccgaatcgta gaaaatctcg tcgaagagat agccggcggc
     6601 aggccggtgc tccgtatctt caaatctgcc caaatctgct tttttcaccg cgcgcggttt
     6661 gccgtcttta ttggtaaatt tggtcggatg gattttgctg ccgacaagcc ggtaatccag
     6721 ttcgaattta tggaaggaat gatgaagcaa aaaccggttg aagccgtttt tgatttgggc
     6781 gatggtttgc atattcaaaa aatcgccaat gttcagttcg ttgcggatgc ggtaaaacgc
     6841 ctgatgcaaa gtctgcatac ggatttttgc cgtttgtgcc agtttttcca gaaactctcg
     6901 gtaagtcatt gtgttgaaac ggataaaatc ttcatcttca atactgtcta tgcggcggga
     6961 aagcataagc ccgttgttga tataagcttc gtttaccgcc gtgcatatgc ctgcctgcgg
     7021 gaatttggcg gcgttttcac gcaaataggc ggtaaataaa tcgacaaatt cggcttcatc
     7081 tttgattttg tactgcaaaa cggctttatg gtgaatcagc tcccacaagg ctttgagttc
     7141 ttcatatttg ccttcgcgca tgatgatggt gtctttgccc tcgtctttgg cgttgctgac
     7201 tttgcctttg tccaaacctt tggggaaggc ttcgggatag gcggctttta atttgtcata
     7261 gccgtcttcg gcaaagtttt cattgtcgtc aatgatgccg tctgaaaaca gttggtttac
     7321 caataccagc ggtttgatat cggggtattt ttgcaatatt ttttgtttca gctcttcggt
     7381 aaactttttg gagatttctt cctgaaaaga attgtcgttg atttcgccga caagctgctt
     7441 cacaaagtct ttttcgctgc tatcgacaaa ataattcagt ttgtacggta catcgcgcac
     7501 ccgcgccatc agctcgttta ccggcaggcg caggccgcgt ccgacttctt gcagcttgga
     7561 agtcgtgctg ccgctggaac gcagtttgca aatctggaaa acgttgggat tgtcccagcc
     7621 ttcgcgcagc gtccatttgg aaaaaataaa gcggcgcggg ttgtccaaag acagcagttt
     7681 ttccttatcg tgcaggattt cattgatttc ctgctcgatt ctatcgtcgc tgtctgtatt
     7741 gtctttggaa aaatagccgc cgtggcaggc ggatacatcg tccaacgtct tttgcaggta
     7801 atcgcggtaa aacgggtcgc tttccgtttt cagacggcgt gccgcttccg cgcgaatcca
     7861 gctttcaaat ttatctttca ggctgcctga aagctcgttg ccgctgcggt agcccgcgat
     7921 atcgtcaata aaaaacagcg tcagcggctt gattttgggc tgtggcgcgc gttctgccaa
     7981 aagcgcgcgt tccagcttga aatgttcggc aaccgcccgc tgcatcatcg catcctgcac
     8041 cgtttgcgaa taggaataag ggttgatgac ggcacccgtt ttcaactcca agccgttgct
     8101 taacaccacc acggttttat tcattttgtc gattttcaaa tccgaaatag ccggatggat
     8161 ttgcgccaaa tcttcgcctt ttgccagttt gaacgtctgc tttttgtcct tttcgtttaa
     8221 ttcaaatttc gcttctttgc cgtccgacga caccagtttt accgccgcat ccatgccgcc
     8281 ctgcatttct tcctgaaaca cgcgcacgcc tttgaccagc ccgtcgttaa acgcgtctac
     8341 ttccgtcaaa cggtaaagca agttgtaata ttcatcgtta aatgttgcac cgtagcgcaa
     8401 aatatattgc ggttttaagc gtttgatatt gccccacgtt ttcgcgctat ctcgggtcgg
     8461 gaatttatgc ggttcgtcca caatcataaa cgggcgcacg gcggccaatg catcaacggg
     8521 attgtcaaac aaatccttca atgccttgtc gcccgtatcg ttcatggacg acgaattaac
     8581 catgcccgcg ttaatcagca gcacatgaat ttcctttttg ttttccgctt tgacaaattg
     8641 ctcaatcgtt atgggcgcat tggacttttt gcccttattc tttttcgcgc tttccaccac
     8701 ataggttttc aggcgtacgc cttcataatc gccgccgaaa tcctgttcaa aatgctctgc
     8761 caaagccttg ctttgcaaaa actgctgtgt tcccgcctta atggacaaag tcggcacgac
     8821 cacgataaat ttgaacacgc ccagccaacg gtgcagctcg aacatggttt gtgtgtaggt
     8881 ataggttttg cccgtgcccg tttccatgga aatatcaagg atattttggt cgtccgaacg
     8941 gtcggggaat cggccgtcta taccgttttg gctttgcact ttcaggatat tgtttgcgta
     9001 ttgttttgca gcaaacaaaa gttcgggatt ttcgtctgcc gtccgatatt tgggcgttgc
     9061 cccgtcaaac acgcccaaaa ccgccgaaac cgcccgcatt tggtgcggct ggtttttctc
     9121 gtaattaaaa ccgctcatga attgcctccg tcaaaccctg ataaccacat tcaactcaat
     9181 ctctttttta ttggcataac cgcgaaccgc ctggtcaagt tcgtgctgca tggcgcttgc
     9241 catattgctg ccgaatacaa tcacgcggtt gggattgaaa gccgcatcgt cgtccagctt
     9301 gcggataaac gccaacaaat cggcagaagt aaaaccggca ttcatcagat acagccgttt
     9361 ttcgcacaga tacgccgtat aactgcccaa ccgcacaggc tcaaccggcg tggtcagtgc
     9421 cgccccgtca tacagtgtcc aagtggtcag aagcgtttgc agctgttctt cgcttaattc
     9481 atcgctaagc ggcaaatccg gttgttcggg cgaaaaatcg ttatccggat gctgcctgaa
     9541 attgtctgcc gtttgaaaga ttttgaagcc caagtcgccg gtgtaatcgg gatgctcggc
     9601 gcggattttg gcggcggctt tttctatgcg ggctttggtg atgtcgaaga tggtcgggta
     9661 gcctgcttta cgggcttcgg atttttcagc ggttttttcg ggaagctgta cacagatata
     9721 gcggcggtta ccgttttgtc cttcggcgtt aagctgcatc acggcgtggg cggttgtgcc
     9781 gctgcctgcg aagaagtcta ggattaggtc attactcttt gaacttattg aaactaaaaa
     9841 tttaatcaat tgactaggct tggggaaggt aaatattttg ctaccaaata aatctgtgat
     9901 ttcttttgtg ccttctttag tcattccgat attttcaggt agcgtcctac taaaaatagc
     9961 caaatattcg gctactgcat cggctaatgt accaacattt tcaggtaatc tactgcttac
    10021 agctactttg ccaaaatcct cgccagcttt tttcatgtcg tcatctttaa agtaacgcat
    10081 aactggattg ctgatattta agaaatcata atcatcaggg aaaacgattt ttcctttatt
    10141 ataataatct tgaaatgtat ctttggttac acgccaagtt gcatttggat ttgctggata
    10201 tttttttcct gtcttgggat caaccattgt gaaaaaacta tttggccttt ccgccgcagt
    10261 tgtttgtttc gttaagtcgt gggtacgcca aggacgatcg gggaaatcat cagtctcata
    10321 atagcgtcgt tccttgcctt tagttgctgc aataaattgg caagattttg cgaatacaaa
    10381 tatccattca taatcctgcg aaataccaaa aggcacatct gatttagctg ttctttttcg
    10441 ccaaggcaat tgtgcaacaa aattcccttc cccaaacact tcatcacaca acaatttcaa
    10501 ctgcgccgct tcgttatcgt caatcgagat aaaaatcacg ccgtcgtcct ttaacagttc
    10561 gcgggcgata tacaggcgcg gatacataaa ggtcagccac gcgctgtgcg agtttgagcc
    10621 tttgtcggtg aaatctaaaa tccgcgcggc ttcgtcttcg tcaatattcg ccaggagggc
    10681 aagttcggcg ggggtgaatt tgcggtcgtc ctgatagaca aagccgtccg agccagtgtt
    10741 gtagggcggg tcgatgtaaa tcatcttcac gctgtttgta taggcgtttt ttaagtgttt
    10801 caacacttct agattgtcgc cacgaatcag caggttttgg ctgcctgcat tttcgggctt
    10861 ggcgttgtgc gtcttatctt cacttatcag ggtttcgggc ggcaggttgc gaagcaggcg
    10921 ggcatatgat ttgcccagcc agttcatttc gtaaaattcg cgtccgatgt cggtctgtgg
    10981 cgcaatttcg gcttgtaatc tgtcgataag gaaatttccg tctgcgtcaa aacaggcggg
    11041 aaacagtttt ttgagctgtt cgagttgggt agagttggcg gtaatgccgt ctgaagtgta
    11101 gattgcctcg gtgttcgccc cggctgtgtc gcttagatca
//
LOCUS       DAXHKX010000047        10956 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHKX010000047 DAXHKX010000000
VERSION     DAXHKX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.047"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(81..1226)
                     /gene="dapE"
                     /locus_tag="ACFMWZ_000998"
     CDS             complement(81..1226)
                     /gene="dapE"
                     /locus_tag="ACFMWZ_000998"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC5934976.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTGIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGRQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTNVARAAIVETCGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLEDIPKLSAVYEGILARLLAGNAV"
     gene            complement(1326..1946)
                     /locus_tag="ACFMWZ_000999"
     CDS             complement(1326..1946)
                     /locus_tag="ACFMWZ_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HFC5934977.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVVKWIAETVEDLLPLYEACHGA"
     gene            complement(1980..2489)
                     /locus_tag="ACFMWZ_001000"
     CDS             complement(1980..2489)
                     /locus_tag="ACFMWZ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC5934978.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQVAMLPDYIAENGC
                     NQIVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLHGN
                     WDAVAAEFAKLEADNAYIPDAEAFKRFVAGYDVHLAIEEPLFDMGKMFIPKEKLTEIG
                     EIMAARRRR"
     gene            complement(2585..3151)
                     /gene="azu"
                     /locus_tag="ACFMWZ_001001"
     CDS             complement(2585..3151)
                     /gene="azu"
                     /locus_tag="ACFMWZ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC5934979.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAPADAAEAPAVGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPK
                     ASMGHNLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPA
                     KLADGDYKFACTFPGHGALMNGKVTLVD"
     gene            complement(3432..3854)
                     /locus_tag="ACFMWZ_001002"
     CDS             complement(3432..3854)
                     /locus_tag="ACFMWZ_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC5934980.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            3985..6735
                     /gene="secA"
                     /locus_tag="ACFMWZ_001003"
     CDS             3985..6735
                     /gene="secA"
                     /locus_tag="ACFMWZ_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC5934981.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPEHGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            6879..8651
                     /gene="dnaG"
                     /locus_tag="ACFMWZ_001004"
     CDS             6879..8651
                     /gene="dnaG"
                     /locus_tag="ACFMWZ_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC5934982.1"
                     /translation="MIPSDFIDELLVKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            8838..10766
                     /gene="rpoD"
                     /locus_tag="ACFMWZ_001005"
     CDS             8838..10766
                     /gene="rpoD"
                     /locus_tag="ACFMWZ_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC5934983.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRRKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         2876 a         3062 c         2692 g         2326 t
ORIGIN      
        1 aatttaatcc actatatctc aaacccacat taggtctaat caaatggtcg gacatccata
       61 tccgacagcc catcttcttt tcagacggca tttccagcca acaaccgcgc caatatcccc
      121 tcatacaccg ctgacagctt cggaatatct tccagccgca cgttttcgtt gatttggtgg
      181 atggtggcat tggacgggcc taattcgatg agttctttcg caatggcttt aatgaagcgt
      241 ccgtccgaag tgccgccggt ggtggacagt tcggtctcca cgccgcaggt ttcaacgatg
      301 gctgcgcgtg ccacattggt tagtttgccc gcatgggtca gaaagggctg ccccgaacac
      361 gaccactgca aatcgtattg cacgccgtgt ttgtccaaaa tggcgtggac gcgttgtttc
      421 agccctgctt cggtggactc ggtggagaag cggaaattga atttgacgtt cagctcgccc
      481 ggaatgacgt tggtcgcgcc tgtgccaccg ttgatattgg aaatttggaa actggtcggc
      541 ggaaagtatt cgttgccttc gtcccatact tcctgcgtca gctctaacaa ggcaggggca
      601 aaagtgtgga cggggttcac cgccaaatga ggataggcaa tatgcccttg cctgcctttg
      661 acggtcaggt tgcccgacag cgagccgcgc cggccgtttt taatcatatc gcccaatttg
      721 tccacggcgg tcggttcgcc gacgatgcag tagtcgataa gctcgtcgcg cgctttcaac
      781 acatcgacga ctttggtcgt gccgtccaac gcgtcgccct cttcgtcgga agtaatcagg
      841 agcgcaatgc tgccttgatg gtcgggatgt tcggcaacga aacgttcgca ggcggtaaca
      901 aaacaggcga tgccggtttt catgtccgcc gcgccgcgcc cgtataatct tccgtcgcgc
      961 tcagtcggtt cgaacggggg cgaatcccat ttttcaacag ggcccgtcgg cacaacgtcg
     1021 gtatgccctg caaaacagac gacgggagct ttcgtgccgc gtcgcaacca gatgtttttg
     1081 gtgtcgccga aatggagttc ttcagccgca aaaccgattt tgtgcaggcg ttcggcaagg
     1141 agtttttggc aatctcggtc gtcgggggta acggatgggc gggaaatcaa ttctttggcg
     1201 agttctaggg attgggtttc ggtcattttt cggcttttct gtttgagatg ccgtctgaac
     1261 ggagtttcag acggcatttg atgttgaatg gaacgggcta acgatagcga agcgggttct
     1321 tcaggtcaag caccatgaca cgcttcgtaa agcggcagca aatcttccac tgtttccgct
     1381 atccatttca cgacatcctg cttgcccaaa tcgtcgcgct cgatgtgttt gccgatgcag
     1441 aagaaatctt cgtcgttttg caaccttcgg tcggattcat tttgctgcgc gacggtgcgg
     1501 tagtcgtcgt attcgctttc cgcaccgtgc cacatatcga atgcagcgta tttgtcggta
     1561 tcgaaatcat ccagccagcg gttgtattcc ggcagtgcaa tcggcgacac gtcggctttg
     1621 tagcaatgcc agtccaagct gacactcaaa cggcggcggt tgagcagtat cgacaaaatc
     1681 gcggcggaat ttttatattg ttcgtatttg aagtaggcaa agaaatgggc gcgaacctgc
     1741 cagccgttgc accagcgttc gatgtgcggc ggcgcaaacg gcgcacccaa ttcggcggca
     1801 acctgctgaa tcagctgctg ccatatctgc cagttttcct tgtagtcggc tttgatttgc
     1861 ggaatgcttt caggctggta ttttttaagc tgggaaaatt ggaaaaacgg gatattgaac
     1921 aaatcgcaac ttttcggggt cagcataata tatccttgag acgattgttt cagacggcat
     1981 tatctgcgcc ggcgcgccgc catgatttcg ccgatttcgg tcagtttctc tttggggatg
     2041 aacatcttgc ccatgtcgaa caagggttct tcaatcgcca aatgcacatc atatcccgcc
     2101 acaaaacgtt tgaacgcttc cgcatcggga atataggcat tgtctgcttc gagtttggca
     2161 aattcggcag ccacggcatc ccagttgcca tgcaggctta catgttggcg taaaagctcg
     2221 tccacgcttt cttgggcttg cggcgcatat tgcagcagga gcgggaaaaa gttttcttct
     2281 tcgtcttcat ggtgcagcgg cgcggcaacg ttgaaatacc gggagatttg gcggatggtt
     2341 tgcaaaacaa tctgattgca gccgttttcg gcgatgtagt ccggcagcat cgcgacttgg
     2401 ccgcaaaaac ggcgtacttt gccgtggcag gcatacagca tttcaatcgg ttcggcaaag
     2461 gtaacgcttt gggtttcaaa cggattcatg ttttcgttct caacggtgca cttttcaagc
     2521 agtcatttta taataaaaca gcctgcacaa agcaggctgt tcgtcttttg aaactttaag
     2581 cggattaatc gaccaaagtc actttgccgt tcatcaaagc accgtgaccc gggaaggtgc
     2641 aggcaaattt gtagtcgccg tcagccaatt tggcaggatc tagagtcagg gaagactctt
     2701 cgccgccgcc gatcagtttg gtgtgggcaa caacgcgcgc atcgtcaggt ttgacatagt
     2761 cggtatcggc agcacctacg ccgtctttaa atacgccgtc catgtcttca gctttggcaa
     2821 tcacaaggtt gtgacccatg ctggctttgg gttgcgtacc ggtatgtttc agagtgatgg
     2881 taaactcttt acatgctttg ctgacttgga tgtctttggt gttgaactgc atattgtcgt
     2941 tggattcgac agttgccgca cagttgccga cagcaggggc ttcggcagca tctgcaggag
     3001 cagcatctgc aggagcagct tcggcggcag gtgcttcgga agcgggtgct tcagcagcag
     3061 gagttgcctc ggcagcaggc gcggcaggtt cttgagagca ggcagccaaa ccgataacgg
     3121 cagcagaaat cagagccaga tacgctttca taacaaatct ccaatcgata aaataatatt
     3181 cggttttaca gaaatcaaag tgcaaccgcc attaacaaaa ccttgaaaaa gattccgccg
     3241 cgttgcacaa acagatgttt cggggtggca ttttgctaca aatttcattt aaaatcaaag
     3301 cctgtttgca agtttacaat cgtttaccca aaaaagggca attttacccc gaacctattt
     3361 ctttagtatt agacccatta tcctttactt cttaatatta acggatgttt acacaaattc
     3421 ccgtatacat tttatgcgcc atgccttctg accaagtttg ccaatgcctc cgccaattcg
     3481 ggatgccgtt tttccaactt taccgccgcc gaaccgaaac tctccagcgc agccttactc
     3541 aaatgcaggg tattggtttt cggcggtttt tccggtttcg ggaccagcct gactgaaaca
     3601 gagcgtatcg aagcatcaag ccctgccaac tgcggcaata ccgacggtgc aatcattttc
     3661 aagcgcgatg ccgccatatt gtttgccgcc aaaaggacaa gcctgccgtc ttcgatacat
     3721 gccgtctgaa aatgcgggtg caggttggca ggcagcagtt ttttcacggc ggcatccaac
     3781 cgccgccact gtcccgcctg tttcaaaagt ccggaaagca gcgcgtcccg cctgcccaac
     3841 tgttccaaat tcataaaaca tacacccaaa aagattgaaa taccgcaaac gcgcctttat
     3901 ttcagacggc attagcactt tgcacaaacg cttgtgttaa aatcgcgttt tcgcccacta
     3961 ttatatcagg cgcaggaatt attcatgctg acaaacattg ccaagaaaat cttcggcagc
     4021 cgcaacgacc gcttgctgaa acaataccgt aaatccgttg ccagaatcaa cgcgctcgaa
     4081 gaacagatgc aagccctaag cgatgctgat ctgcaagcca aaactgccga attcaaacaa
     4141 cgcctcgccg acggtcagac tttggacggc attttgcccg aagccttcgc cgtctgccgc
     4201 gaagcgtccc gccgcaccct cggtatgcgc cacttcgacg tgcagcttat cggcggtatg
     4261 gtgctgcacg acggcaaaat cgccgaaatg cgtaccggcg aaggcaaaac cctggtcgcc
     4321 accctcgccg tctatctcaa cgcgctggcc ggcaaaggcg tacacgtcgt taccgtcaac
     4381 gactacctcg cctcacgcga tgcgggcatt atggagccgc tctacaattt cctcggcctg
     4441 accgtcggcg tgattatttc agatatgcag ccgttcgacc gtcaaaacgc ctatgccgcc
     4501 gatatcacct acggcaccaa taatgaattc ggcttcgact acctgcgcga caatatggta
     4561 accgaccaat acgataaagt gcagcgcgaa ttgaattttg ccgttgtcga tgaagtggat
     4621 tccatcttga ttgacgaagc gcgcactccg ctgattatct ccggccaggc agacgacaac
     4681 attcagttgt accaaatcat gaacaccgtt ccgccccacc tcgtccgtca agagacagaa
     4741 gaaggcgaag gcgactattg ggtcgacgaa aaggcacatc aggtcatcct gagcgaagca
     4801 ggtcacgaac acgccgagca aatcctgacc caaatgggat tgctggcaga aaacgactcc
     4861 ctctattccg ccgccaatat cgccctgatg caccacctta tggcggcatt gcgcgcgcat
     4921 tccctcttcc acaaagacca acattacgtc atccaagacg gcgaaatcgt catcgtggac
     4981 gaattcaccg gccggctgat gtccggccgc cgctggtcgg agggtctgca tcaagccgtc
     5041 gaagccaaag aaggcgtgga aatcaaacgc gaaaaccaaa ccctcgcctc catcaccttc
     5101 caaaactatt tccgcctgta caccaagctc tccggcatga ccggcacagc cgataccgaa
     5161 gccttcgagt tccaaagcat ctacaacctc gaaaccgtca tcatcccgac caaccgcccc
     5221 atacaacgca aagacttcaa cgaccagatt ttccgttccg ccgaagaaaa attcgaagcc
     5281 gtcgtcaaag acatcgaaga atgccacaaa cgtgggcagc ccgtcctcgt cggcaccacc
     5341 agcatcgaaa actccgaact ggtctcccac cttctgcaaa aagccggact gccgcacaac
     5401 gtcctcaacg ccaaagaaca cgaacgcgaa gccctgattg tcgcccaagc cggcaaagtc
     5461 ggcgcgatta ccgttgccac caatatggcg ggacgcggta cggacatcgt tttaggcggc
     5521 aacctgaagc accaaaccga tgccatccgt gccgacgaaa ccttgagcga cgaagagaaa
     5581 caggcacaaa tcgccgcact cgaaaacggc tggcaggcag aacacgataa agtaatggaa
     5641 gcaggcggcc tgcacatcat cggtacggaa cgccacgaaa gccgccgtat cgacaaccaa
     5701 ttgcgcggac gttccggccg tcagggcgac cccggatcca gccgtttcta cctctcgttt
     5761 gaagacccgc tgctgcgcct attcgcactc gaccgcgccg ccgccatcct caaccgtctc
     5821 gcccccgaac acggcgtcgc catcgaacac aacctgctga cacgccaaat cgaaggggcg
     5881 caacgcaaag tcgaaggcag aaacttcgat atgcgcaaac aggttttgga atacgacgac
     5941 gttgccaacg aacagcgcaa agtcatttac agccagcgca acgaaattct gaccagcaaa
     6001 gacatcagcg acctgatgaa ggaaatccgt tctgatgtcg tcagcgacct cgtggatacc
     6061 tatatgccgc ccgacagcat ggaagaacaa tgggacatcc cgactttaga gaaccgtctg
     6121 gctgccgaat tcagactgca cgaagacatc caatcttggc tgaaggcgga caatgcgatt
     6181 gacggtcaag acatcaaaga gcgcctgatc gaacgcatcg aaaacgaata tgccgccaaa
     6241 accgaactgg tcggcaagca ggcaatggct gatttcgagc gcaacgtgat gttgcaggtc
     6301 atcgacaacc aatggcgcga acatcttgcc gccatggact acctgcgaca aggcatccac
     6361 cttcgcagct atgcccagaa aaatccgaag caggaataca aacgcgaagc ctttacaatg
     6421 ttccaagacc tgtggaacgg catcaaattc catatcgcct ccctgcttac ctcggttcaa
     6481 atcgaacaaa accctgtcgc ggctgttgaa gagcaacctg tcggcaacat ccagtccatc
     6541 cattccgaat cgcccgatat ggaagaactt ttgggtcaat cgcaaaccga tctggttacc
     6601 gaagccttta atcccgatgg gacggatttc agccccgaag ccttggaagc gcgggggcaa
     6661 atcgtccacc gcaacgaccc ctgcccctgc ggcagcggtt tgaaatacaa acaatgccac
     6721 ggcaaattgg cttaagcgtt tgaacgcaaa tgccgtctga acatccggtt tccagcttca
     6781 gacggcattt tgcctgaatc gccgtatccg accgacaatc cgaaaactcc cgatttgata
     6841 ccatccgaac taaaaacagg catccgcccc gccgaactat gattccatcc gacttcattg
     6901 acgaacttct agtcaaaacc gatattgtcg atattatcga cgagcaggtt ccgctgaaaa
     6961 aaggcggggc gaactatatg gcgtgttgcc cgttccacaa ggaaaaaacg ccgtcgtttt
     7021 cggtcagtcc aaccaagcag ttttaccatt gtttcagttg cggggcgcat ggttcggcga
     7081 ttggttttgt gatggagcat caggggctgt cgtttccgga ggctgtgcag tttcttgccg
     7141 accgtgtggg catggttgtg cctaaagtgc gtgggcagaa cgataatccc gaagtccgtg
     7201 ccgaacgtaa gaaaaaacag cagacgctgg aagaaacgac ggcggcggcg gctgattttt
     7261 acgcgcaaca gctaaaattc aatccggcgg caaaagctta tttggacaag cgcggcttga
     7321 gtgcagaagt tatcgcgcat tatggtttgg gctacgcgcc cgacggctgg cagcctttgg
     7381 cgcaagtgtt ccaaccgtat ccgaataccg cgttggtgga tacggggatg gtgattgaca
     7441 atgaggggcg gtattacgac cgcttccgcc atcggattat gttccctatc cgcaatccgc
     7501 gcgggcaggt catcggtttc ggcggcaggg tgctggacga ctccaaaccc aaatatctaa
     7561 actctcccga tacacctttg tttgacaaag ggaaaaacct ttacggcttg tatgaggggc
     7621 gtgccgctgt caaggaagcg gggcggattt tggtggtcga aggctatatg gacgtggtcg
     7681 cgctggcaca gttcggcgtg ggctacggtg tggcggcctt gggcacggca acaacagctg
     7741 aacacgtcaa aatcctgatg cgtcaggcgg acagtattta tttctgtttc gacggcgaca
     7801 gcgcggggcg caaagcggct tggcgcgcgc tggaaaacgc gctgccgcag ttgaaggacg
     7861 acaaatcgct gcattttttg ttcctgccgg aagaacacga ccccgacagt tacatccgtg
     7921 cctacggcaa agcgcaattt gaagatgcgc tgttgaatca aagcaagccc ttgtcggaat
     7981 atttttggga acatcttaca gacggcattc atctcaatac gcaggaaggc aaggcggaat
     8041 tggtaaaaac cagttcgccg cttttggcgc agattaccgc gccggcattg gcttatttgt
     8101 taaaacaacg gcttagcgag ctggtcggca tcgaccccga caacctcgcg caactgctcg
     8161 gacaggaagc gccaaagcgg cacgtcaaac aaaaaaacta caaactgccc ccgatttccg
     8221 tcaaacagcc cgttatgccg acattggtgc aacggcaaat ccgcagcctc ttgataaatc
     8281 cggattgggc tgcatatata gacctgcccg attatttggc gttggacggc gatttcgcct
     8341 gccttgccaa cctcgccgaa accattaaaa accatccttc cgtgccggca accgcacagg
     8401 ttttggaaca tatgcgcggt tcgccctacg aagaaacaat caaccgcatc ttccggtcgg
     8461 cccttcaatc ggaagaaatg gaaggcggcg gcgaagaaga ttgcgaaaac ttccaaatcg
     8521 gcatcaaaaa actgctcaat gagttaaaat acagccaaat cgaaacatta aaacaaaaaa
     8581 gcctgcaatc cggcttaaat gaaagcgaga aaaaactttt gctgtcgctg ctgaccgcaa
     8641 aacaaaattg accggcggat tccgccatcc gtaaaccgtt atgccgtctg aaaagcattc
     8701 accccggctg caacaacgac acctgcagaa cacccatccc caaaagcctt cagacggcat
     8761 cagagtaccc tactctgcca cgccttcagg tgcgtccaaa cgcaaaccgt cggcatcttg
     8821 ccaacagaaa gcagacaatg tccaaaaacc aaaatcacga agaataccaa gacgatgccc
     8881 gccccctgac gattgaggaa caacgcgcgc gcctgcgtca gctcatcatc atgggtaaag
     8941 aacgcggcta catcacctac tccgaaatca acgacgccct gccagacgat atgtctgatg
     9001 ccgaccaaat agacaatatc gtcagcatga tttccggttt gggtatccaa gttaccgaac
     9061 acgcccccga tgcggaagac atattgttaa gcgacaatgc cgccgttacc gacgatgatg
     9121 ccgtcgaaga agccgaggcc gccctttcca gcgccgactc cgagttcggc aggacgaccg
     9181 accccgtccg tatgtatatg cgcgaaatgg gacaggtcga cctgctgacc cgcgaagacg
     9241 aaatcatcat cgccaaaaaa atcgaaaacg ccctgaaaaa tatggttcag gccatctccg
     9301 cctgcccggg atccattgcg gaaatcttag aactcatcga aaaaatccgc aaagacgaaa
     9361 tccgcgtcga cgaagtcgta gaagccatta tcgacccgaa tgaagtattg ctcaacgaat
     9421 tgggcttggg gcacttggaa accacagcac ccgagaaacc ttccaacgac aattcggacg
     9481 aaaacgaaga cgacgaagaa tcggaagaag atgcggatga aatctcggca gccaatctcg
     9541 ccgaattgaa acaaaaagtc atcggccact ttgcccaaat cgaaaaagac tacaaaaaaa
     9601 tgatcggccg tttggaaaaa caccacagcc ggcacaaaga ctatctcgcc taccgcgacg
     9661 cgattgccaa caaactgctg gaagtccgtt tcgccacccg gcaaatcgac agcctcagca
     9721 gcagcctgcg cagaaaagta gaaaacatcc gcaaactcga acgcgaaatc cgcgacatct
     9781 gcctcgaccg cgtccatatg gagcgcgact acttcatcca aaacttcctg cccgaaatca
     9841 ccaatctgga atggattgaa gaagaaatcg ccaaaggcag ggtttggagc gacgcgctcg
     9901 accgcttccg ccacgccatc ctcgaaaaac aaaccgagtt ggcggatatg gaaaaagaaa
     9961 cccgcatttc catcgaagag ttgaaagaaa tcaacaaaaa tatggtgtcg agcgaaaaag
    10021 aaaccgcagc cgccaaacag gaaatgattc aggcaaactt gcgcctcgtg atttccatcg
    10081 ccaaaaaata taccaaccgg ggcttacaat tccttgatct gattcaggaa ggcaacatcg
    10141 gtttgatgaa agcggtcgat aagttcgaat accgcagagg ctataaattc tccacctacg
    10201 caacctggtg gatccgccag gcaatcaccc gctcgattgc cgatcaagcg cgtaccatcc
    10261 gcattccggt acatatgatt gaaaccatca acaagatgaa ccgcatctcg cgccaacacc
    10321 ttcaagaaac cggcgaagaa cccgattccg ccaaacttgc cgaactgatg cagatgcccg
    10381 aagacaaaat ccgcaaaatc atgaaaatcg ccaaagagcc gatttcgatg gaaaccccca
    10441 tcggcgacga cgacgattcg cacttgggcg acttcatcga agatgccaac aatgttgcgc
    10501 cggccgatgc ggcaatgtac accagcctgc acgaagtaac caaagaaatc ctcgaaagcc
    10561 tgacaccgcg tgaggcaaaa gtcctgcgta tgcgtttcgg catcgatatg aacaccgacc
    10621 acacgctgga agaagtcggc agacagtttg acgtaacgcg cgaacgcatc cgccaaattg
    10681 aagcaaaggc actccgcaaa ttacgccatc cgacaagaag cgaccggctg agaagtttct
    10741 tggacagcga agacagcaaa ctataaccct caaaaaaacc gcaggtttca aatacctgcg
    10801 gttttttctt acacaataaa caacgcttcc acatatccca cactcctatc ccgagacctt
    10861 tgcaaaattc cccaaaatcc cctaaattcc tactaagaca tttaggggat ttctcatgag
    10921 caccttcttc caacaaaccg cccaagccat gattgc
//
LOCUS       DAXHKX010000048        10865 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHKX010000048 DAXHKX010000000
VERSION     DAXHKX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.048"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(10..1101)
                     /gene="mltB"
                     /locus_tag="ACFMWZ_001006"
     CDS             complement(10..1101)
                     /gene="mltB"
                     /locus_tag="ACFMWZ_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC5934984.1"
                     /translation="MKNRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAVNANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1098..1313)
                     /locus_tag="ACFMWZ_001007"
     CDS             complement(1098..1313)
                     /locus_tag="ACFMWZ_001007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934985.1"
                     /translation="MRIAGKYGGRQCRLKGEVPSDGIFGDDIRISGGVKGNVRHSAKP
                     PIAADAAFRAAPLAPLSHLPYLFGTYL"
     gene            complement(1304..2731)
                     /locus_tag="ACFMWZ_001008"
     CDS             complement(1304..2731)
                     /locus_tag="ACFMWZ_001008"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC5934986.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYIDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYTLENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDAAACLLNDIRKDILDCRYCG"
     gene            2916..7043
                     /gene="mfd"
                     /locus_tag="ACFMWZ_001009"
     CDS             2916..7043
                     /gene="mfd"
                     /locus_tag="ACFMWZ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC5934987.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKQLKVVLTQDAE
                     QALRLQTAWLFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADIFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSEEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESRLPPGKSNLHGQIQQQPTPSP
                     VGEGWGEGKAVAAQTEFSATATNPLPNPLPHEREQSAAAVSDDLKAKSSLHLVASNLH
                     RQIRQQPTPSPVGEGWGEGKAVAAQTEFSAAATNPLPSPLPQEREQSAAVVSDSLKAA
                     AVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVAR
                     SRVHNRRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLITMDLGGETNEMMLL
                     EYAGEAQLYVPVSQLHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTAAELL
                     NLYAQRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVC
                     GDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSR
                     FNNSKATKAALEGLADGTVDIVIGTHKLVQDNIRFKSLGLVIIDEEHRFGVCQKEQLK
                     RLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREA
                     VLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQ
                     QRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTP
                     EYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTE
                     MLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCET
                     VQQINAIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNN
                     NVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            7192..7575
                     /gene="panD"
                     /locus_tag="ACFMWZ_001010"
     CDS             7192..7575
                     /gene="panD"
                     /locus_tag="ACFMWZ_001010"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC5934988.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVVCLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7597..8439
                     /gene="kdsA"
                     /locus_tag="ACFMWZ_001011"
     CDS             7597..8439
                     /gene="kdsA"
                     /locus_tag="ACFMWZ_001011"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC5934989.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8454..8894
                     /locus_tag="ACFMWZ_001012"
     CDS             8454..8894
                     /locus_tag="ACFMWZ_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC5934990.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     SVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSSKEAV"
     gene            8941..10227
                     /gene="eno"
                     /locus_tag="ACFMWZ_001013"
     CDS             8941..10227
                     /gene="eno"
                     /locus_tag="ACFMWZ_001013"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC5934991.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDAGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            10241..10519
                     /gene="ftsB"
                     /locus_tag="ACFMWZ_001014"
     CDS             10241..10519
                     /gene="ftsB"
                     /locus_tag="ACFMWZ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC5934992.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10560..10850)
                     /gene="yfaE"
                     /locus_tag="ACFMWZ_001015"
     CDS             complement(10560..10850)
                     /gene="yfaE"
                     /locus_tag="ACFMWZ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC5934993.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYRKPPLAFLGRDEILPCCCYVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         2602 a         3205 c         2774 g         2284 t
ORIGIN      
        1 taaaaaacat tacaatcccg ggccgccaag cgaattggca atgtccctga ccgccgttac
       61 atacatccga ctgtgattgt actgccatac cgtataaaaa ttgttcaagc ccaaataata
      121 ttcaaacacg ccgggtgcgg tttccagttt gaacaaaacc gccttttcat catcggcaag
      181 ctcttcgccg gggatgatgc cgtacgcctt caaatccgcc accgtccgcg tcagggcggt
      241 tttttcgcca atgattgcct gaacatccgc acccggcgcc aatgttgcag acaccagtat
      301 tttcccgccc gtgcgccaac cgtgctgctt catataattg gcaaccgatg ccgcgacatc
      361 gccgatgttg ccccatatgt cccgatgtcc gtccccgtca taatccaccg cccatttccg
      421 gtagctcgaa ggcataaatt gcggcatccc cattgcgccc gcatagctgc ctttaaaggc
      481 gaaaacatcg ccgccttctt cttttgccag ctttaaaagc tcgaccaatt ctttttggaa
      541 aaacccggcg cggcgggggt aatcaaagcc taaggtcgcc aatgcgtccg ccacacggaa
      601 actgcccgta tttttaccgt aattcgtttc aatcccgata accgccacga taagttcggc
      661 aggcacgccg tatttttgcg ccacatcatc gataagtgcg cggttttccg cataaaaccg
      721 gcgcgcgccg cgaaatttcg ccttgcccga atttcccgtg cggaacacat accacggacg
      781 cgatgtggag gggcggtgca taatcttgac gatgtccgcc ttgtaagccg ctttgtcaaa
      841 aaaatcctgc cattccgccc gggaaaaatc ccctttcccg acttcatcgt ccacaaaacg
      901 gcggacattt gcattgacgg caaacccgct gtcggatacc ggtacggctg ccgcgtcaaa
      961 cgcggggcgg ctttcttttt tcatttcatc cgcgcggggg gcttgggctt catttgcccg
     1021 gggcgggcgt gcctccatcg ccgtacaggc agacaaagcc gccaaacaaa ttgccagcgg
     1081 cagtattttt ctgtttttca taaatatgtt ccgaacaaat agggtaagtg ggaaagcggc
     1141 gcaaggggcg cggcgcgaaa tgcggcatcc gccgcaatcg gcggctttgc ggaatgccgc
     1201 acgttgcctt ttacgccgcc tgaaatccgt atgtcgtcgc cgaaaatgcc gtctgaaggc
     1261 acttccccct tcagacggca ttgcctgccg ccgtatttgc ccgctatccg caatatcggc
     1321 agtccaatat gtctttgcgg atgtcgttca gcaggcaggc tgccgcgtct tcgtgtttcg
     1381 cctgtacgaa gacaaagagt cgggcgatga ttttgccgat aaagactttt tgcagggcgc
     1441 aggcatcggt cagggtgtgt tcctgcagga agctgcggat gtcttcaggc aaagtgtagt
     1501 cgcgggcgac ggcggcaaag cgggcatcgg tttgcaggcg gtcgagcagg gtttggtttt
     1561 tgtccaactt catgcctgct tctttttctt cggcggtggc gcggacaaac tggtcgtaaa
     1621 tttcggcata aagcatatcg ttcgggcctt caaaaatcgt gaaggggcgg atgtcgatag
     1681 cgatattgcc ggcggtgtgt ccgcgttcaa aacccttcgc gcccaagagt ttttgcaaca
     1741 tttgcgcggc ggcgtaagtg tattccgtgg cgagggtttt gacgatgttc gcctccatca
     1801 gttgatgggc gacgggtgca acgggcgaaa cggaatggca gacgtagcgg taaagaatct
     1861 cggaaacctg atggcggcgc cggatttcgc ggcgttcgta atcgacgaat ctgatgtcgt
     1921 tgcggacgta tcgttccagg ttttcaaggg tgtattccat aatgccgtgc gtcatgccga
     1981 tcagttgcag gcggctgcgg ataaagatgt tttggaacgc gcgcaaaccg gcagcgtcgc
     2041 tctgggagag tttcatcacg gcggttgcag gcatttcggc atcgatgcgg ttgacggcgt
     2101 aacggacggc gcgcaagcct tcggatgcga gggtttcgca gcggatgtat gttttgggga
     2161 cgagcagcag gtcgatgact ttggcgagtt tgccgttttt gcgctctttg gcggcaacga
     2221 ggaggaagtc gctttgcgag ttgccctgcc agtatttcgc ggcgttgacg taaatggttt
     2281 gtccgtcgat atattcgtag taggactgca tttcgcgtgc aatcgccgcg ccggaggttt
     2341 cgggttcggt aacacccaaa ccgccgccct cgcctttgaa aatcatctcc aaaccttgcg
     2401 cgacttgcgc ttcatcgccg aactcttgca gtggctgcaa caccagcgcg ccttcgatgc
     2461 cggtacgcag cgtaacgggc acgccgtaat gccccgcaat ccgcaggact tcttggattt
     2521 caaactggct gcccttgcgc ccgccgtatt ttttgtcgag gaagggcaac agcaaacccg
     2581 cctgcttcaa ggcaagccat ttgtcttcgg gcaggtatcg catcaggtcg ataccgtctg
     2641 aaaaaatgcg gcggaatgcg gattcgatgt gctttaaaaa agcagccgtg tccatagttg
     2701 acggctgcgc gctcggttcg gtgtgtatca tcggcttcct ctgtcggttc ccattaatcg
     2761 gcggccggtc aaaccgcctg ccacagttta gagttgattt tctaaacttt accacaaagt
     2821 gcgccgggca acaatccgcc gacctttcag acggcatcgc gtcccctccc gtgctaaaat
     2881 gaccgtttgc atcactgtcc gccgattgcc gcactatgac ctaccccatc cccaaacccc
     2941 gtgaaaaatc ccgttggccc aatctttcgc aaggctcgct gcccttggct ttggcgcgtt
     3001 atctgccgca taagcagctc aaggttgtct tgactcaaga tgcggaacag gcgctacgcc
     3061 ttcagacggc atggctgttt ttccgtccgc acgatacggc ggtgttcttg ccggactggg
     3121 aaacgctgcc ttacgagcgt ttttcgccgc atcaggattt agtgtcggag cgcttgtcgg
     3181 cattgtggca gattaaaagc ggcgcggcgg acgtgttgtt cgtgccggtt gctacggcga
     3241 tgcagaagct gccgcctgtg ccgtttttgg cggggcgcac gttttggctg aaaacagggc
     3301 agactttgga tataggccgt ctgaaaagcg atttggtgga tgcaggttat aaccatgttt
     3361 cccacgttgt tgcggcaggc gagttcgctg tgcgcggcgg cattgtcgat ttgttcccca
     3421 tgggcagcga aatgccctac cgcatcgatt tgtttgacga tgaaatcgac agcatcaaaa
     3481 cctttgatac ggacacgcag cgcaccatct cccccgtttc cgaaatccgc ctgctgccgg
     3541 cgcacgaatt ccccaccgac agcgaggcgc aaaaaatctt ccgcagccgc ttccgcgagg
     3601 aagtcgatgg taatccgaac gatgcggctg tgtacaaagc agtaagcaac ggccacttcg
     3661 gtgcgggcgt ggaatattat ctgccgctgt tttttgaaaa cgagctggaa acgctgtttg
     3721 actatatcgg cgaagatgcg ctgtttgttt cattgggcga cgttcacgcc gaggcaaacc
     3781 gcttttggag cgacgttaaa tcacgttacg ccatggcgca gggcgacgaa acctatccgc
     3841 ctttgcttcc acagcatttg tatctctctg ccgatatttt tgcaggccgt ctgaaaaact
     3901 acggacaagt gctgcccgat gtttccggca aggaacacat cctgcccgac cttgccgtca
     3961 accgccaatc cgaagagccg ttgcaggcat tgaaggattt tcagacggca tttgaaggac
     4021 ggattttgct gtgcgccgaa agtttgggac ggcgcgaaac catgctcggt ttcttgcagc
     4081 aaaacggttt gaaagccaaa cctgtgtccg actggcaggg cttcttgtcg gcgcatgagc
     4141 cgctgatgat tacagttgcg ccgttggcat acgggttcaa actgggcgga ctgcaatcgc
     4201 cgaaccaaca gcaaactact tccgcctccg aaggagaagg cgatgcggtt accgaccaaa
     4261 ccgaattttc cgcatccgca acaaaccctc tccctagccc tctcccacag gagagggaac
     4321 aaagtgcagc cgtcgtttca gacagtctga aagcagccgc cgtttcaacc gaaagccgcc
     4381 tgccccccgg taaaagtaat ctgcacgggc aaatccaaca gcaacctact ccctcccccg
     4441 tgggggaggg ttggggagag ggcaaagcag ttgccgctca aactgaattt tccgcaaccg
     4501 caacaaaccc tctccctaac cctctcccac atgagaggga acaaagtgca gccgccgttt
     4561 cagacgacct gaaagccaaa agcagcctgc atctcgtcgc aagtaatctg cacaggcaaa
     4621 tccgacagca acctactccc tcccccgtgg gggagggttg gggagagggc aaagcagttg
     4681 ccgctcaaac cgaattttcc gcagccgcaa caaaccctct ccctagccct ctcccacagg
     4741 agagggaaca aagtgcagcc gtcgtttcag acagtctgaa agcagccgcc gtttcaaccg
     4801 aaagcagcct gccccccggt aaaagtaatc tgcacgggca aatccaacag caacctgccc
     4861 cctcccccgt gggggagggt tggggagagg gcaaagcagt tgccgctcaa agtgccatcg
     4921 ccgtcatcac cgaatccgat ctctaccaat acgtcgcccg ttcgcgcgtc cacaaccgcc
     4981 gcaagaaaca cgccgccgtt tcagacggtc tgttgcgcga ccttgccgaa atcaacatcg
     5041 gcgaccccgt cgttcacgaa gaacacggca tcgggcggta tatgggcttg atcacaatgg
     5101 atttgggcgg cgaaaccaac gaaatgatgt tgcttgaata tgcaggcgaa gcgcagcttt
     5161 atgtgcctgt ttcgcaactg catttaatca gccgctactc cggtcaggcg catgaaaacg
     5221 tcgccctgca caagctcggc agcggcgcgt ggaacaaagc gaaacgcaaa gccgccgaaa
     5281 aagcgcgcga taccgccgcc gagttgctca acctctacgc ccaacgcgct gcccaatcgg
     5341 gacacaagtt tgaaatcaac gagttggact atcaggcgtt tgccgacggt ttcggctacg
     5401 aggaaaccga agaccaggcc gccgccatcg ccgccgtgat taaagacctg acacaggcga
     5461 agccgatgga ccgactcgtg tgcggcgatg tcggctttgg caaaaccgaa gtcgccctgc
     5521 gcgccgcgtt tgtggcggtg atgggcggca aacaggtcgc cgtactcgcc ccaaccacgc
     5581 ttctagtcga gcagcacgcg caaaactttg ccgaccgttt cgccgatttc cccgtgaaag
     5641 tcgccagcct ttcgcgtttc aacaacagca aagccaccaa agccgcactg gaaggcttgg
     5701 cggacggcac ggtcgacatc gttatcggca cgcacaaatt ggtgcaggac aacatcagat
     5761 tcaaaagctt aggtttagtg attatcgatg aagaacaccg cttcggcgtg tgccagaaag
     5821 aacagctcaa acgcctgcgc gccaatgtcg atatccttac catgaccgcc acgccgattc
     5881 cgcgcaccct cagcatggca ctcgaaggcc tgcgcgactt ctcgctgatt accaccgcgc
     5941 ccagccgccg cctcgccgtg aaaacctttg tcaaaccgtt cagcgaaggc agcgtgcgcg
     6001 aagccgtgtt gcgcgaactc aaacgcggag gacaggtatt tttcctgcac aatgaagtag
     6061 atacgattga aaatatgcgc gagcggctgg aaaccctgct gcccgaagcc cgcatcggcg
     6121 tggcgcacgg acaactgcgc gagcgcgagc tggaacaagt catgcgcgac tttttacagc
     6181 aacgattcaa cgtgttgctc tgttccacca tcatcgaaac cggtatcgac attcccaacg
     6241 ccaacaccat catcatcaac cgcgccgaca aattcggact ggcgcaactg caccagcttc
     6301 gcgggcgcgt cggacgcagc caccaccaag cctacgccta cctgctcacg cccgaataca
     6361 tcaccaaaga cgcagaaaaa cgcctcgacg ccatcgcggc agcagacgaa ctcggcgcag
     6421 gtttcaccct cgccatgcag gatttggaaa tccgcggcgc aggcgaaatc ctcggcgaag
     6481 ggcagtccgg cgaaatgata caggtcggct tcacgctcta caccgaaatg ctcaaacaag
     6541 ccgtgcgcga tctcaaaaaa ggccgccaac ccgacctcga cgcgccgctg ggcatcacca
     6601 ccgaaatcaa actgcacagc cccgccctac tgcccgaaag ctactgcccc gacatccacg
     6661 agcgtctcgt cctctacaaa cgcctcgccg tctgcgaaac cgtgcagcaa atcaacgcca
     6721 tacacgaaga actcgtcgac cgcttcggcc tgcccgaaca acccgtcaaa acccttatcg
     6781 aaagccacca cctgcggctt atggcaaaag aattgggtat cgatgccatt gatgcggccg
     6841 gcgaagcggt aacggtaacc ttcggtaaaa acaataatgt cgatccaacc gaaatcatcc
     6901 tgctgattca gaacgacaaa aaataccgcc ttgccggcgc cgataagctg cggtttaccg
     6961 cagagatgga aaatatcgag gtcagaatca acaccgtaaa aaacgtttta aaaaccttgc
     7021 aaaacagatg cctgcccaaa taaagccgac accgcaatgc cgtctgaaac accgttttcc
     7081 ttgtccgaaa gccgccatta tgaatttgaa ggaaactcca ctataatacg gcattcagat
     7141 ctccagacgg catcgcgccc gtcagaccgc acacaaacca aaaggaaata catgttccgt
     7201 actatgcttg gcggaaaaat ccaccgcgcc accgttaccg aagccgattt gaactacgtc
     7261 ggcagtatta ccgtcgatca agacctgtta gacgcagcag gcatctaccc caacgaaaaa
     7321 gtcgccatcg tcaacaacaa caacggcgaa cgttttgaaa cctataccat ttcaggcaaa
     7381 cgcggcagcg gcgtggtctg cctgaacggg gcggcggcca ggctggtaca gaaaggcgat
     7441 atcgtcatca tcatgtctta cgtccaactc tccgaacccg aaatcgccgc acacgaaccc
     7501 aaagtcgtct tggtagacgg aaacaacaaa atccgcgaca tcatctccta cgagccgccg
     7561 cacaccgtgc tgtaaccctg aaaacggaca ttgaccatgg atattaaaat caacggcatt
     7621 accgtcggta acgatgctcc tttcgtactg ttcggcggca tcaacgtttt agaagatttg
     7681 gattccaccc tccaaacctg tgcgcattac gtcgaagtta cccgaaaact cggtattccc
     7741 tatatcttta aagcctcttt cgacaaggca aaccgttcct ccatccattc ttatcgcggc
     7801 gtaggcttgg aagaaggctt aaagattttt gaaaaagtca aagcagagtt cggcatcccc
     7861 gtcattaccg acgtacacga accccatcag tgccaacccg tcgccgaagt gtgcgatgtc
     7921 atccagcttc ccgcctttct tgcgcggcag accgatttgg tggccgcaat ggcggaaacg
     7981 ggcaatgtca tcaacatcaa aaaaccccag ttcctcagcc cttcgcaaat gaaaaacatc
     8041 gtggaaaaat tccgcgaagc cggcaacggg aaactgattt tatgcgaacg cggcagcagc
     8101 ttcggctacg acaacctcgt tgtcgatatg ctcggtttcg gcgtgatgaa acaaacctgc
     8161 ggcaacctgc cggttatttt cgacgttacc cattccctgc aaacccgcga tgccggttct
     8221 gccgcatccg gcggtcgtcg cgcacaggct ttggatttgg cacttgcagg catggcaacc
     8281 cgccttgccg gcctgttcct cgaatcgcac cccgatccga aactggcaaa atgcgacggc
     8341 cccagcgcgc tgccgctgca ccttttagaa gattttttaa tccgcatcaa agcattggac
     8401 gatttaatca aatcacaacc gattttaaca atcgagtaac acggtttcgc cttatgatgc
     8461 agactttccg aaaaatcagc ctgtatgccg caaccttgtg gctcggtatg cagattatgg
     8521 cgggttatat cgtcgcgccg gtgctgttca aaatgctgcc caaaatgcag gcgggcgaaa
     8581 ttgccagcgt attgttcgac atcctctctt ggagcgggct tgccgtttgg ggcgcggtac
     8641 tggctgccgc ctttgccgcc ctaacccggc ggcaaaccgc cctgctgctt tttttattgt
     8701 ccgcccttgc cgccaaccga ttcttgatta cacccgttat cgaggcgctg aaatacgggc
     8761 atgagaattg gctgttgtcg tttgtaggcg gatccttcgg aatgtggcac ggcatttcca
     8821 gtattgtttt tatggcaacc gccctacttt cagcagtttt aagttggcgg ctttccagca
     8881 aagaggccgt ctgaagccct cccatttttt acctcccttc acttcacttg gagaacattc
     8941 atgagcgcaa tcgttgatat tttcgcccgc gaaattttgg actcacgcgg caaccccaca
     9001 gtcgagtgtg atgtattgct cgaatccggc gtaatgggac gtgcagccgt accgagcggt
     9061 gcgtccaccg gtcaaaaaga ggctttggaa ctgcgcgacg gcgacaaatc ccgttattcg
     9121 ggcaagggcg tattgaaggc ggtcgaacac gtcaacaacc aaatcgccca agccctcatt
     9181 ggtatcgatg ccagcgaaca atcttatatc gaccaaatca tgatcgaatt ggacggtact
     9241 gaaaacaaag gtaacttggg tgccaacgcg actttggccg tttctatggc cgtagcacgc
     9301 gcagccgctg aagatgcagg gctgccgctt taccgctact tgggcggcgc aggtccgatg
     9361 tccctgcccg taccgatgat gaacgtcatc aacggcggcg aacacgccaa caacagcctg
     9421 aacatccaag agtttatgat tatgcccgtc ggcgcaaaat ctttccgcga agcgttgcgc
     9481 tgcggtgcgg aaattttcca cgccttgaaa aaactgtgcg acagcaaagg cttcccgacc
     9541 acagtcggcg acgaaggcgg tttcgccccc aacctgaaca gccacaaaga agccctgcaa
     9601 ctgatggtcg aggcgaccga agccgccggc tacaaagcgg gcgaagacgt attattcgca
     9661 ttggactgcg cctccagcga gttctacaaa gacggcaaat accacttgga agccgaaggc
     9721 cgctcctaca ccaacgcgga atttgccgaa tatctggaaa gtctggtcaa cgagttcccc
     9781 atcatctcca tcgaagacgg catggatgaa aacgactggg aaggctggaa actgctgacc
     9841 gaaaaactgg gcggcaaagt ccaactcgtt ggcgacgacc tcttcgttac caacccgaaa
     9901 atccttgccg aaggcattga aaaaggcgtg gcaaacgcac tattggtcaa agtcaaccaa
     9961 atcggtactt tgagtgaaac cctgaaagcc gtcgacttag ccaaacgcaa ccgctacgcc
    10021 agcgtaatga gccaccgctc cggcgaaacc gaagacagca ccattgccga cttggcagtc
    10081 gccaccaact gtatgcagat caaaaccggt tctttgagcc gttccgaccg catggcgaaa
    10141 tacaaccaac tgctgcgtat cgaggaagaa ttggcggaag ccgccgacta ccccggcaaa
    10201 gccgcattct accaactggg caaataaaaa aggttaaggt atgaagtggg taactgtcgt
    10261 tttatccttc gcacttgtct gttgccaata cagcctctgg ttcggcaaag gcagcatcgg
    10321 acgcaacagc agtctgagag aacagattgc cgttcaagaa gaaaaaaacc agacactcgc
    10381 cctacgcaat cattcccttg ccgccgaagt ctatgatttg gaaaacggtc aagaagccat
    10441 ttcggaaatc gcccgggtag aactgggtta tatccaagac ggtgaaacct tttaccgact
    10501 catcaggcat aaccggtaat accgtcaaaa agccgtccga accaatgttc ggacggcttt
    10561 tatttcaaca aactgtcaga cagcccctca tcctcccccg acaaaccgca atccagcctg
    10621 acatccccct cgacgtaaca gcagcacggc agtatctcgt cccgccccaa aaaagccaaa
    10681 ggcggcttcc gataagtaac gcttccctcc aaaatcttca cccggcacga tccgcaatat
    10741 ccgcttcggc actgatattc caccatatgc cccgtccgtt ccaagccttc caacagggtc
    10801 tcgccctcca agagctcaaa aaaaccctta ttcgtaccaa tgcgcgccat ttccgaccaa
    10861 tcaaa
//
LOCUS       DAXHKX010000049        10561 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHKX010000049 DAXHKX010000000
VERSION     DAXHKX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.049"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(235..2643)
                     /locus_tag="ACFMWZ_001016"
     CDS             complement(235..2643)
                     /locus_tag="ACFMWZ_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC5934994.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            2713..3717
                     /gene="amgK"
                     /locus_tag="ACFMWZ_001017"
     CDS             2713..3717
                     /gene="amgK"
                     /locus_tag="ACFMWZ_001017"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC5934995.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLSFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLDNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASRKGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(3774..4472)
                     /locus_tag="ACFMWZ_001018"
     CDS             complement(3774..4472)
                     /locus_tag="ACFMWZ_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC5934996.1"
                     /translation="MKSRQLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            4499..4690
                     /locus_tag="ACFMWZ_001019"
     CDS             4499..4690
                     /locus_tag="ACFMWZ_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5934997.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGRFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            4690..6228
                     /gene="murJ"
                     /locus_tag="ACFMWZ_001020"
     CDS             4690..6228
                     /gene="murJ"
                     /locus_tag="ACFMWZ_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC5934998.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYAQQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            6360..7316
                     /locus_tag="ACFMWZ_001021"
     CDS             6360..7316
                     /locus_tag="ACFMWZ_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC5934999.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     AVSAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRRTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            7367..8149
                     /gene="trpC"
                     /locus_tag="ACFMWZ_001022"
     CDS             7367..8149
                     /gene="trpC"
                     /locus_tag="ACFMWZ_001022"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC5935000.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNTEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDESELEKCLNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIEAEVGKLF"
     gene            8214..10514
                     /gene="recQ"
                     /locus_tag="ACFMWZ_001023"
     CDS             8214..10514
                     /gene="recQ"
                     /locus_tag="ACFMWZ_001023"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC5935001.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADEAREIAD
                     RLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMAGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKRKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2157 a         3007 c         3029 g         2368 t
ORIGIN      
        1 ctttggcagc tttggcagct ttggcagctt tggcagcttt ggcggatgcg gtttttgcct
       61 tttgcggtgc ggcgtggaca tcggctgctg ccagcaatgc ggcggcaatc atcagggctt
      121 tgattttcat cattctttcc ttatcgtttg aatcctgcct ttgatggcgg gtttggggtt
      181 gtcggtggaa tgccgtcttg tgcgggaacg gtgctcctgc cgggtttccg acggtcaggg
      241 ccgtttgttg cgtccggcgg aaagagagtg ggcggggatg tagccgggaa cggcgacatc
      301 catcctgtct gcggggtttc tgccgacact gctgaggtct ttcaactgaa gtgagaaaaa
      361 gacagcgttt ttgtaggtgt tttcgccggt aacgtagcgt tgggcgtaca cgcccgcgcc
      421 ccagcagccg caactgcttt tgtattccgc acccgccagc acctctatcg gttttttggc
      481 ttcaaaaccg tagttgtaac ggacgacggc cgacaggttg cgcgtcagcg gccattgtgc
      541 ggacaggtcg agctggctga gtttgtcgta aaaataggaa ccgtcggact tcaggtagat
      601 tttttcgttg cgcccgtatt tgtagcgggc gttcagcact ttgccctgtg cgggacggta
      661 gcttgcaccg acggcgtagt tctcggcgcg tttgtcgttt tggttgtagt ggatgctgct
      721 gtcgaggatg aagcggctgc cgatgctgcc ggaggcaaat gccacccagt cggaacggtt
      781 gcgcggtttt ttgccgacgc tgccgtcaag catcaccgca tcatccttga aatagaattt
      841 ctgaccgatg ccggcgcgga aacgctcttc ccccgtcgcg ccgtccaaaa tacggctttg
      901 cacggcggcg gaaaggctgt ttgcggtgtt aatcctgtcg ttgccgtaat agaggttttc
      961 gcgaaagagc tgcccgtagc cgaagctgct ttccgacgaa tcgaaattgg gcaggtcatt
     1021 ttgggatttg gcaggaatat agttgtagaa caggcgcggc tcgagggttt gcaggactcc
     1081 gccgccgaac atccgcgtat tccgctcaaa agttgcgccg ctgtcgatgt tgacaatggg
     1141 cagagtgcgg ctgacgcgtc gggcttcttg gctgccgaag cggttgaggc tgtaataggt
     1201 ggcgtgcagt ccgagtttgg gacggacgta accccagctg ttgctgaaat cccatttgat
     1261 gtcgggatag acgacgaggc ggctgccgtc ttggcggctg tcgtggctga agcgggtaaa
     1321 ttgtgcggac acgccgattt gcgccctgcc ggtgtttttg cgccaatcgg cggaaaggcg
     1381 cggcatcagg gcatacggtt tgtctttgta gccgctttgg tttgccagcg tctggtattt
     1441 cagaaccgaa aggccggcat tcaggctgcc gcccgccgcc ctgccgccat aatccagcca
     1501 tacacggcgg ttgaggttga cgttgccggc gatttctttg ttgccgtaaa agtcgcggta
     1561 gtagccgctg tcggagactt ggttgaaatc gacacccgcc tgaagcgtgt cggaaatgtc
     1621 gtgccgatgc tgccatttcg cctgatagcg gttattcctg ccgcttttct tgtcgtgcgg
     1681 cagccaggtc aggtcggact ggccggcata atccggccgc aggtagcgta cctgcccgtc
     1741 aaagaccgcg ccgcgttcgc cgatcacgcc gggcgcgaac gtggcatcga gattgggggc
     1801 aaggttgaaa taatagggaa cggaaaggga aacgccgtcc gaaccggcgg acagtgaggg
     1861 aacaagcagg ccgcttttgc ggttgccgtc aagcgggaag tccgcccaag gggtgtagaa
     1921 aatgggaacg ccgccgaaca cgaaggcggc gtgtttggca acgcctatgc ctttttcccg
     1981 atcggcttcg acggaagcgg ccttgacata ccagccggca tcgccggcgg aacaggtgtt
     2041 gaattgggtt tccgtcagtt tgtaatgccc ttcgcccaac atttcggcgg tgcggctgac
     2101 gctttgcagc cgccgtccgc cgtgttcggt ttccatgcgg acgttgtgcg cttcgccggt
     2161 ctgttgctcg agattgtagg tcagggtttc gccccgaatc agcgtaccgt cctgttggag
     2221 ggcgaaccgg tcgcctgcgg taacggtgtc gcccgactgg tcgtaatccg cccaatcggc
     2281 attgagggtc gtccggttgc gttcgacgac gacgttgcct tcggcacgca cctgcacctg
     2341 cgactgtcct tccatcctgt cggcaacaat gcgcgtatag tcttcgggga tggatgcttc
     2401 gccgctgcct tggacggcgg cttcggttct ctcggggctg ccgctttcgt tactgcaaaa
     2461 caggcaggtc gaaccgaggc tcaggctggt aggctgtatg ggttcggaca cgctccgaac
     2521 gcttcctccg gcggtcggat tgtccgtttc ctccgccgca acggcatcgg cagcggcgca
     2581 atgcgtgccg aagcagaagc ccaatgccag caccagtggt ttgagtgaaa ataaacgagc
     2641 caaaatcgcc cctcaagtcg gtttgccagt taaaatagcg tttattgtaa cctgaaatgc
     2701 tttagtactg ttatgcaacg acaaatcaaa ctgaaaaatt ggcttcagac cgtttatccc
     2761 gaacgggact tcgatctgtc cttcgcggcg gcggatgctg atttccgccg ctattttcgt
     2821 gcaacgtttt cagacggcgg cagcgtcgtc tgtatggacg cgccgcccga caagatgagt
     2881 gtcgcgcctt atttaaaagt gcagaaactg tttgacatgg tcaatgtgcc gcagatattg
     2941 cacgcggaca cggatttggg gtttgtggta ttgaacgact tggacaatac gacgtttttg
     3001 accgcgatgc ttcaggaaca gggcgaaacg gcgcacaaag ccctgctttt ggaggccatc
     3061 ggcgagttgg tcgaattgca gaaggcgagc cgtaaagggg ttttgcccga atatgaccgt
     3121 gaaacgatgt tgcgcgaaat caacctgttc ccggaatggt ttgtcgcaaa agaattgggg
     3181 cgcgaattaa cattcaaaca acgccaactt tggcagcaaa ccgtcgatac gctgctgccg
     3241 cccctgttgg cgcagcccaa agtctatgtg caccgcgact ttatcgtccg caacctgatg
     3301 ctgacgcgcg gcaggccggg cgttttagac ttccaagacg cgctttacgg cccgatttcc
     3361 tacgatttgg tgtcgctgtt gcgcgatgcc tttatcgaat gggaagaaga atttgtcttg
     3421 gacttggtta tccgctactg ggaaaaggcg cgggctgccg gcttgcccgt ccccgaagcg
     3481 tttgacgagt tttaccgctg gttcgaatgg atgggcgtgc agcggcactt gaaggttgca
     3541 ggcatcttcg cacgcctgta ctaccgcgac ggcaaagaca aataccgtcc cgaaatcccg
     3601 cgtttcctca attacctgcg ccgcgtatcg cgccgttatg ccgaactcgc cccgctctac
     3661 gcgctcttgg tcgaactggt cggcgatgaa gaactggaaa cgggctttac gttttaaacc
     3721 caatcaaaat gccgtctgaa aaccaagttt cagacggcat ttttcaaacg ggcttactgc
     3781 gcggcttttt gttcttcgcg tactttgtcc gccaaaaggt cgatggtgtt cataccggac
     3841 tcccagtcgg caaattcaac tttatattta ccgccgacga taaccgtggg cgtaccgtcg
     3901 atttggaagg tttcggtcag ctcctgcatt ttgtcggcgc gcgcctggct ttcgggggac
     3961 tcgtaggcgg caaggacttt tttgccgtca aaggcggttt gttcgcccag ccattttttg
     4021 aggacttccg gctcttgcag cttgattttt tggttgacca tcgcatcgaa aatatggctg
     4081 ttcgccacat ctttgctgtc ggcggcagcc atatcgacgg cggcggcgag gcgtgccagc
     4141 ggcagcattt ctttttgcca aacgacatgc tcggtacgca ggtacatatc gtctttaaaa
     4201 gacttggcgt gtttgctcaa aacaggttcg aggtgggcgc agtgcggaca gaaatagccg
     4261 aaaaactcaa ggacttcgac tttgcctgcc tgctgttggg gaatcgggtt ggcaaggacg
     4321 gtatagtttt gcccttcgac cagccctgcc ggggcggcgg ctgccgaagc ggcaggcgcg
     4381 ctgtcggcgg ggacgctggt ttggactttg ctgtcgcacg cggcaagggc gaacagggcg
     4441 gcaacgccga gggcgagttg tctggatttc atacggctct ccgtgatgtt ggaaacagat
     4501 gttggaaatc aatcggaatc cggctatttt attgcatttc cgcgtgttga tacagtttgc
     4561 cgccggaaaa ggacgttttc gtttcgggaa accgcttcag acggcatcaa acccgatgcc
     4621 gtctgaagcg gtttctgtcg tacaatacgc gccgttgccc caaacgggta cgactgttga
     4681 ggaacaatga tgaatatgct gggagctttg gcaaaagtcg gcagcctgac gatggtgtcg
     4741 cgcgttttgg gatttgtgcg cgatacggtc attgcgcgcg cattcggcgc gggcatggcg
     4801 acggatgcgt tttttgtcgc gttcaaactg cccaacctgc ttcgccgcgt gtttgcggag
     4861 ggggcgtttg cccaagcgtt tgtgccgatt ttggcggaat ataaggaaac gcgttcgaaa
     4921 gaggcgacgg aggcttttat ccgccatgtg gcggggatgc tgtcgtttgt gctggtcatc
     4981 gttaccgcgc tgggcatact tgccgcgcct tgggtgattt atgtttccgc acccggtttt
     5041 gccaaagatg ccgacaaatt tcagctctcc atcgatttgc tgcggattac gttcccctat
     5101 atcttattga tttcactttc ctcttttgtc ggctcggtac tcaattccta tcataaattc
     5161 agcattccgg cgtttacgcc cacgttcctg aacgtgtcgt ttatcgtatt cgcgctgttt
     5221 ttcgtgccgt atttcgatcc tcccgttacc gcgctggctt gggcggtttt tgtcggcggc
     5281 attttacaac tcggcttcca actgccctgg ctggcgaaac tgggtttttt gaaactgccc
     5341 aaactgaatt tcaaagatgc ggcggtcaac cgcgtcatga aacagatggc gcctgcgatt
     5401 ttgggcgtga gcgtggcgca gatttctttg gtgatcaaca cgattttcgc gtcttatctg
     5461 caatcgggca gcgtttcatg gatgtattac gccgaccgca tgatggagct gcccggcggc
     5521 gtgctggggg cggcactcgg tacgattttg ctgccgactt tgtccaaaca ctcggcaaac
     5581 caagatacgg aacagttttc cgccctgctc gactggggtt tgcgcctgtg catgctgctg
     5641 acgctgccgg cggcggtcgg actggcggtg ttgtcgttcc cgctggtggc gacgctgttt
     5701 atgtaccgcg aatttacgct gtttgacgcg cagatgacgc aacacgcgct gattgcctat
     5761 tctttcggtt taatcggttt aatcatgatt aaagtgttgg cacccggctt ctatgcgcag
     5821 caaaacatca aaacgcccgt caaaatcgcc atcttcacgc tcatctgcac gcagttgatg
     5881 aaccttgcct ttatcggccc gttgaaacac gccgggcttt cgctcgccat cggtttgggc
     5941 gcgtgcatca atgccggatt gttgttcttc ctgttacgca gacacggtat ttaccaacct
     6001 ggcaagggtt gggcagcgtt cttagcaaaa atgctgctct cgctcgccgt gatgggaggc
     6061 ggcctgtatg ccgcccaaat ctggctgccg ttcgactggg cacacgccgg cggaatgcaa
     6121 aaggccgccc ggctcttcat cctgattgcc gtcggcggcg gactgtattt cgcatcactg
     6181 gcggcactgg gcttccgtcc gcgccatttc aaacgcgtgg aaagctgacc gatgccgata
     6241 tcctttttcg gcaaacgctt ttccacgcct atattcgggc gcgcatcccc cacgtccgca
     6301 ccagccggac gggctgccgc atcatcaatg ccgtctgaaa acaagaaaac cgatacctta
     6361 tgattttaac accgccggac acgccctttt tcctccgcaa cggcaatgcc gacacgattg
     6421 ccgccaaatt cctgcaacgc cccgcaccca cataccgccg cgagctgctt cccgacagca
     6481 cgggtaaaac caaagtcgcc tacgattttt cagacggcat ttcgcccgat gcgccgctgg
     6541 tcgtgctgtt tcacggtttg gaaggaagca gccgcagcca ttacgcggtc gaactgatgc
     6601 tcgcggtacg cagccgcggt tggcacggcg cagtcgtcca tttccgcagc tgcggcggca
     6661 ttgccaacac cgctccggtg ttctaccact tgggcgatac cgccgaaatc gcctttactt
     6721 tggacacgct tgccgcgcgt taccgtgaaa tatacgccgt cggcgtatcg ctgggcggca
     6781 acgcgctggc aaaatatttg ggcgaacggg gcgaaaacgc gctgccgcaa gccgctgccg
     6841 ccgtatccgc ccccgtcgat gcagaggcgg caggcagacg cttcgacagc ggcatcacgc
     6901 ggctgctcta cacgcgctac ttcctccgca ccctgatacc caaagcacgg tcgctccaag
     6961 gttttcagac ggcatttgcc gcagggtgca aaacactggg cgagtttgac gaccgtttca
     7021 ccgcaccgct gcacggcttt gccgaccggc acgactacta ccgccgaact tcctgcaaac
     7081 cgctgctcaa acacgttgcc aaaccgctgc tcctgctcaa tgccgtcaac gaccccttcc
     7141 tgccgcccga agcgttgccg acaccggacg aagtgtccga agccgttacc ctgttccagc
     7201 cggcacacgg tggtcatgtc ggctttgtcg gcagcaccgg cggcaggctg cacctgcaat
     7261 ggttgccgca gaccgtcctg tcctatttcg acagcttccg cacaaacagt cgttaacggt
     7321 ttgatgctaa tattccccct ttccccagac gaatacggaa cccgacatga ccgacatcct
     7381 caataaaatc cttgccacca aagcacagga agttgccgcc caaaaagccg ccgtcaacac
     7441 cgaacacatc cgcgcacttg ccgcagaagc cgcgcccgtc cgcagcttca tcgattcgat
     7501 acgcggcaaa caccgcctca acctgcccgc cgtcattgcc gaaatcaaaa aagcaagccc
     7561 gagcaaaggc ctgatccgtc cggacttccg accggccgag attgcacgcg cctatgaaaa
     7621 cgccggagcg gcgtgtttgt ccgtactgac cgacgaaccc tatttccaag gttcgcccga
     7681 atacctcaaa caggcgcgcg aagccgtatc gctgcccgtg ctgcgcaaag acttcatcat
     7741 cgacgaatat caggtttatc aggcgcgcgc ctggggggcg gatgccgtcc tgctgattgc
     7801 cgccgcactg gaacaggaac aattggaacg ctttgaagcg gtggcgcacg aattgggcat
     7861 gaccgtcctg ctcgagctgc acgatgaaag cgaattggaa aaatgcctca acctgaccac
     7921 gccgttgtgg ggtgtaaaca accgcaacct gaggactttt gaagtctccc tcgaccaaac
     7981 cctgtcgctg ctgcccgcac tggaaggcaa aaccgtcgtt accgaaagcg gcattacggg
     8041 taaggcggat gtggaattta tgcgggcgcg cggcgtgcac accttcctga tcggcgaaac
     8101 gttcatgcgt gccgacgata ttgaagcaga agtgggcaaa ctcttctaaa tcccgatttc
     8161 agacggcata ttgccgccga ccgatcaaca tccttaccga gcacggaacc atcatgcacc
     8221 gaccgaccgc caaacagatt ttgcacgaag tattcggtta tcccgaattt cgcggcaggc
     8281 aggaggctgt catcaatact ttggcaggcg gcgggagttt gacggtgctg atgccgacgg
     8341 gcggcggcaa gtctttgtgt taccagattc ccgcgctgat gcgcgaaggc gtggcggttg
     8401 tcgtatcgcc gctgattgcg ttgatgaacg accaagtggc aaacctgcac gccgccggca
     8461 tcgaggcggc ggcagtcaac agcggcacat cggcagacga ggcgcgcgag attgccgacc
     8521 ggcttgccca aggccgtctg aaactgcttt atgtcgcacc ggaacgcttg gttaccgacc
     8581 gctttttgcg ttttctcgac caacaaaccg tcagcctgtt cgccattgac gaagcgcact
     8641 gcgtcagcca gtggggacac gatttccgcc ccgaatacca acagctcggc atgcttgccg
     8701 aacgctatcc gaacgtcccg cgcatcgccc tgaccgccac cgccgatgcc gccacgcgcg
     8761 ccgacatcaa gcattatctg cacttggacg atgcgcccga atttatttcc agcttcgacc
     8821 gcccgaatat ttattatcag gttatcgaaa aaaacaacgg caaaaaacaa ctgcttgaat
     8881 ttatccgcaa agaaatggcg gggcaaagcg gcattgtgta ttgcctaagc cgcaaaaagg
     8941 ttgaagatgt ggcgcagttt ttgcgtgaaa acggattaaa cgcgattccg tatcatgctg
     9001 gtttgagcat ggacgtgcgc gaggaaaacc aacgccgctt tacgcatgaa gacaatatta
     9061 tcgtggtggc gaccgtggcg ttcggcatgg gcatagacaa acccgacgtg cgctttgtcg
     9121 cccatctcga tatgccccag agtgtcgaac atttctatca ggaatcgggg cgtgccggca
     9181 gggacgggct gcctgccgcg agctggctgt gttacggctt gaacgattgg gtgttgctgc
     9241 gcgaacggat tgccgaaggc aacagcgatg aggtgcaaaa gcaaatcgaa atgcaaaaac
     9301 tcgatgccat gctttccgtc tgcgaaaccg ccgcctgccg ccgcgtactg ttgctcaaac
     9361 attttggcga agaatccgaa ccctgcggcc attgcgacaa ctgcctgcat ccgcctgtgc
     9421 ggtttgacgg cacggtgttg gtacaaaaat tactcagctg cgtgtaccgc gccggacaac
     9481 gttttgccgc cggttacatc accaaccttt tgcgaggtaa aagcgacgat tggatacgcg
     9541 gcaaccggca cgaacaactg tccacattcg gcatcggtac ggagttgtcc gacaaagaat
     9601 ggcgcagcgt catccgccag tgtatcagcc tcggctacct caccgtcaac attacccgat
     9661 atcaggcatt gcaactgacc gaagccgcca aaaaagtcct caaaggcgaa accgaagtga
     9721 tgctgcgtcc gctcaagcgc gacaagcccg ccgcccgcac cctcaaagac aactggctgc
     9781 gtaccgaacg cgaagaacgc ctgtggcagg cattgcgcgt ttggcgtatg aaacaggcag
     9841 aagccgaagg catccccgcc tatatgattt tcggcgacaa aaccctgcgc gaccttgtcg
     9901 aaaaaatgcc gcaagacctc aacggtctgc acgacatcta cggcttgggc gaagccaaaa
     9961 ccgaacgttt cggacacggc atcctcgaag tctgccgcaa cgccgccggc ttcagccgcg
    10021 acgcggtcat ccgtccgcaa accgaacgcg aacaacaact gcgtcaaaaa ctcgaagcct
    10081 ggcggtatga acaggcaagg gcggaaaact gcgccctgca tgccgtcctc tccgacgaaa
    10141 gccttgccga tatgcttgcc gatacgccag aaaccgaaac cgacctcgaa ggcgtgcacg
    10201 gcttgggcag cgtacgcgcc gccaaatacg gacgggacat ccttgccgtc tgccgtccgt
    10261 tttcagacgg catcgatgaa accgccaaac gcaaacgccg cctgatgcgc gccctgatcc
    10321 aatggtgcaa cgaaacggca aaacacgaac agtccgaacc ctaccgcatt ctcagcaaag
    10381 ccgccctgcg cgccattgcc gccaaacagc cggaaggctt ggcggagctt gccgccgtat
    10441 acggcgtagg cgaagaaaaa gccgcacgtt acggtgcggc ggtgttggcg gtattggaac
    10501 gaaatgccgt ctgaagcccg ttaccaagtt tcagacggca ttgcctctat ttaaaaattc
    10561 c
//
LOCUS       DAXHKX010000050        10418 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHKX010000050 DAXHKX010000000
VERSION     DAXHKX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.050"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(4..1005)
                     /locus_tag="ACFMWZ_001024"
     CDS             complement(4..1005)
                     /locus_tag="ACFMWZ_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC5935002.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSQSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEAYEPPAHWEWIRKIRDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
     gene            complement(1082..4915)
                     /locus_tag="ACFMWZ_001025"
     CDS             complement(1082..4915)
                     /locus_tag="ACFMWZ_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC5935003.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETIQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLACWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKHTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKARYGSA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFEPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASNLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5176..5272
                     /gene="ffs"
                     /locus_tag="ACFMWZ_001026"
     ncRNA           5176..5272
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFMWZ_001026"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5439..7130
                     /gene="dld"
                     /locus_tag="ACFMWZ_001027"
     CDS             5439..7130
                     /gene="dld"
                     /locus_tag="ACFMWZ_001027"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC5935004.1"
                     /translation="MSASQLLSRLTQTVGEKYIITAPAKTEQYRQGYRFGEGRALAVV
                     RPGTILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7262..7369
                     /locus_tag="ACFMWZ_001028"
                     /pseudo
     CDS             7262..7369
                     /locus_tag="ACFMWZ_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7425..7796
                     /locus_tag="ACFMWZ_001029"
     CDS             7425..7796
                     /locus_tag="ACFMWZ_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC5935005.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            7951..8499
                     /locus_tag="ACFMWZ_001030"
     CDS             7951..8499
                     /locus_tag="ACFMWZ_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC5935006.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            8635..9726
                     /locus_tag="ACFMWZ_001031"
     CDS             8635..9726
                     /locus_tag="ACFMWZ_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC5935007.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10184..10354
                     /locus_tag="ACFMWZ_001032"
     CDS             10184..10354
                     /locus_tag="ACFMWZ_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC5935008.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2392 a         2888 c         2831 g         2307 t
ORIGIN      
        1 tattcaagta ttcatttctc gctcaaattc agccaagatg ttccgaactt cgtccgcatc
       61 cttaaccgtt cggacgcggt cgaacagatt ttgtgctgct gcaaattctt tcttcatcat
      121 acccagccac tgtttcagcc gcgccagcgg atatttgttg tttgcctctt ttgtcaggca
      181 cagctcgaaa aactgccgta tccatttgga aacttcggca aaatccgtgt ctttgaccgg
      241 cccgccgttc tcgtattgct tgatttgccg cgccaaatcg gggcggatga ccgcgccgcg
      301 accgagcatc acgctgttgc agccgctgat tgttttgatg ccgatatagt cctgcaggct
      361 gaaaacgtcg ccgttggcgg taacgggaat attgacggta tctcgaattt tccttatcca
      421 ttcccaatgc gccggcggtt cgtaagcctc ggctttggta cgcgcgtgta cggtcagtcc
      481 gcatgcgccc ccttcggcaa tcgcacaggc gcattccaaa gcccggcttt tatcttcata
      541 gccgagccgc atttttgcgg tgagcggaat atgtgcgggc aaacgtccgc gcagcgtttt
      601 gacgatgtgg aatatcagtt ccggctcttt taaaaggatt gcgccgcctt tgtgtttgtt
      661 gacggtgggc gcggggcagc cgaagttcaa atcgattttg ttcgcaccga agcggacggc
      721 ttccagcgca ttcgccgcca tattgtccgc atcgctgccc aaaagttgga cggtgcaggg
      781 cgtgccggaa aacgttttgt ttccgttggc gatttcgggg acatatttta accatatgga
      841 ttgggaatgc acggtatggg taatgcgtac aaattcgctg acgcattcgt cgtagccgcc
      901 aatacgcgtc agcaggtcgc gcatcacgtc atcgaccaga ccctgcatag gggcaaggat
      961 gattcgggtt ttttgtttgg gttcggttgt ttgtccgtca atcatgattc gggtaggtat
     1021 ttgattcaaa caatgccgtc tgaaggcttt cggcgttcag acggcatttc cgtgtccgtt
     1081 gttacagcaa tactttttcc acgccgccgt tgttggcttt tttcacaaac tcgtctagcc
     1141 agttttcgcc gaggatgtgt ttcgccattt cgatgacgat gtagtcggca ggcatattgt
     1201 tgtcgtcggt gtagcggctc aagccttgca ggcaggcggg gcaggaggtc agcattttga
     1261 cgggttcgcc ctgcggcagc tctttgaggt ttttctcgat ttcttcctgt ttgcggaatt
     1321 tgacttgggt ggcgatgtcg gggcgtttga cggcaaacat accggattcg ccgcagcagc
     1381 ggtcgcttaa aacgactttc tgccccatca ggttgcttgc catttgggtg gcgttcatgg
     1441 ttttgatggg tgtgtggcag gggtcgtggt agaggtattg ctgacctttc acgccgtcga
     1501 gtttcacgcc tttttcaagc aggtattcgt ggatgtcgat gatgcggcag ccggggaaga
     1561 tttcctcaaa tcggtatttt tcgagctggt cgtaacaagt gccgcaactg acgacgacgg
     1621 ttttgatgtc gaggtagttg agggtgtttg ccatacggtg gaaagccacg cggttgttgg
     1681 tgctcatttc ttcggctttt gccttattgc cgcccgcgtc ctgcggatag ccgcaacaca
     1741 tatagccggg cggcaggacg gtttgcacgc cgacgtgcca gagcatggct tggacggcga
     1801 gtccgatttg gctgaacagg cgttccgaac cacaacccgg gaagtagaac acggcttcgg
     1861 catcttcggg cgcggcgggg ttgcggatga tggggatgct tttgccgtct tcgatgccca
     1921 ataaggagcg cggtgttttg gcgggtacgc ttttcggcaa aggacggttg atgaaatgga
     1981 taacctgttc tttaattggg gctttgccga cggttgcctt gggttcggct ttttgctttt
     2041 tcgtgccgat cggaagaagc ttgccgattt tgtaggcaaa gttttgcgct gggaagccgg
     2101 tctgtatcat ggcggcgcga agggctttga tggttttcgg gccggtggcg ttcaaaaacg
     2161 ccatacccat agctgcggca ggctcaaatc gtttgtggcc ggaatcggca aggtagttgc
     2221 ggacggctac ggttacgtcg ccgaagtcga tgttgacggg gcagggtttg acgcagcggt
     2281 ggcacacggt gcagtggtcg ccgatgtcca tgagttcttc aaagtgtttg atggaaacgc
     2341 cgcgacgggt ttgttcttcg tataagaagg cctcggtcag caaacccacg ccgaggattt
     2401 tgttgcgggg gctgtacagc aggttggcac gcggaacgtg ggtggagcag acgggtttgc
     2461 atttgccgca gcgcaggcag tctttgacgg aatcggcaat cgtgccgagg tctgattttt
     2521 ccataatcag cgattccgcg cccaaaagct cgaaagacgg cgtgtaggcg ttgcgtaagt
     2581 ccgagccttt catcagtttg tgacggttga aggtgtgttt gggatcgact tggtttttgt
     2641 agtcccaaaa cggctgcatt tcttcgtcgc ttaaaaattc gagcttggtg atgccgatgc
     2701 cgtgttcgcc cgaaatcacg cctccgagcg aacgggcgat tttcataatg cgttcgactg
     2761 agcggtatgc cgtctgaagc atttcggcat cgtctgagtt aaccggaata ttggtgtgaa
     2821 cgttaccgtc gccggcgtgc atatgcaggg cgacaaagac gcggctgcgt acggtttttg
     2881 cgtggatttt tcccaagcct tggataattt tggtgtcggt tttgccgctg aagatttcag
     2941 aaagcggctt cattacgtcc gctttgacag acacgcgcag gcggaaatcg cggaatgcgg
     3001 taaagcagct ttcattgtct ttggcttcgg gcgcggcgtg gacggctgag ccatagcgtg
     3061 ctttatagtc ggcaagcggc gcgtccaagt tggcaagcag ccattcccaa cgcgctttga
     3121 cggcggaaac gtgggcaagg gcgtgtttgc cgcgttcgcc caacagttcg gcggtcggca
     3181 ggtctgttcc cattttgtcg atggggagtt tgcccgaaag atattgctcc aatgcggcac
     3241 agagtttgag tttgttttgg atggaaagct cgatgttgat gcgttcgatg ccgtctgaat
     3301 actcgccgag cctttcgagt gggatgacca cgtcttcgtt aattttaaag gcgttggtgt
     3361 gcttggcgat ggcggcggtg cggctgcggt cgagccaaaa ggttttgcgc gcttcgggag
     3421 acacggcgat aaagccttcg ccatcgcggg cgcgggcaag ttcgcaaatg tgttcggcgg
     3481 ctgcctctac ggcggcttcg tcgtcggaaa ccacgtccgc cagcaagacc attttcggcc
     3541 gtcccttgcc cgccgctttg gttgcgtagc cgacggcgcg gacgtaacgc cagtccaaat
     3601 gttccaaacc cgccagccgc acgctgtcgt gggcaagcag gaaatcgcgg atttcgacga
     3661 tagaaggcgt agcggtggcg accgtaccga aaaactccat acacacggtg cgcgtgtgtt
     3721 tcggcatttt gtgcaacacg aaggcgacgc tggtgatgat gccgtccgtg ccttctttct
     3781 gcacgccggg taggccgctc aagaatttgt cggtaacgtc tttgcccaaa ccgactttgc
     3841 ggaatttgtg gccgggaatt tctaagcgtt cggttttaac gatattgagg ccgtctgaat
     3901 ccagcgtgtg aacgtcgaat acggcggttt cttcgtcgtg gattttgccg aaattgtggc
     3961 gcacgcgctc gatgcgcagc cattcaccct gcgggttgac catctgccag caggcaaggt
     4021 tgtctaacgc cgtcccccac agtacggctt ttttgccgcc cgcgttcatc gccacattgc
     4081 cgcccacgca ggacgcgtcg gcggaagtcg gatcgacggc gaacaccaaa cctgcctgat
     4141 gcgcggtttc ttccactcgt cgggttacta cgcccgctcc gcaccggata atcggatgct
     4201 tgccgtccaa gcccgccagc tcaacgtatt caacaccacg atgcttgtcg agtttttcgg
     4261 tattgatgac cgcgctgttc gcgtccaaag gtaccgcgcc gccggtataa cccgtgccgc
     4321 cgccgcgcgg gataataacc aagtccaatt cgattaaggc gcgcaccaaa ggcgcgattt
     4381 ccgcctccgt gtcgggattg acgacgacaa aaggatattc gacgcgccag tcggtcgcat
     4441 cggtaacgtg cgttacgcgc gccaatccgt caaacatgat gttgtgcggc ttggtaattt
     4501 tgcttaagcg ttccaaaatt tgatggcgtt tttggatggt ttcatcaaaa ctgctatcaa
     4561 aacgctccac tgctttttct gctgcggcaa ccaacacatc gacttgccga ttatcgtcgc
     4621 ggcgtttgcg gatttcgttc aacctgtggc gcatttcgcg taccagcgcg gcgcggcgtt
     4681 tcgggtgctc cagcaaatca tcgaccagat acggattgcg cacgaccacc caaatatcgc
     4741 ccaatacttc aaacagcatt cgtgccgaac gcccggtttt acgctgtccg cgcaggtctt
     4801 gcaggatttg ccacgcttcg tcgcccaata atcggatgac gatttcgcgg tcggtgtagg
     4861 atgtatagtt gtaggggatt tcccgaatac gctgcggggc ggtagtcgtg gtcatggtgt
     4921 gtccgtgttt ggcagttttt atttgaatgc cgtctgaaaa gtcggtttca ggcgggtttg
     4981 aaaaggcgat aatgtagttc aatttttgat atttttcaat gccgaaacag gaaataccct
     5041 tatagaaaca aaataacgct ttgagaaatt gttgacaata ttatcaaata taaattttag
     5101 gtggtgtttc cggcgtatga ccgtatcaaa acggttgagt ttttcagaaa acccgttata
     5161 atgccgtttc tctgaggcgg gtctccccgc atggcaaatc ggaacaccgg gtcaggggcg
     5221 gaagccagca gcccactccg gtgcgccagt gccgggggtt tggcccgccg ccctatttga
     5281 aacgccggag cttcgatgtt ccggcgttcc cgtatcgcac cgatgccgtc tgaaagccgt
     5341 tcagacggca ttttctttac tttgccataa cgtataacta caatgtgaaa acaattccaa
     5401 tgtgcaatgc acctgccgac aattttaagg aaactgccat gagcgcatca caattactgt
     5461 caagactgac ccaaaccgta ggcgaaaaat acatcatcac cgcccccgcg aagaccgaac
     5521 aataccgcca aggctaccgc ttcggcgagg gcagggcgtt ggcggtggtg cgccccggaa
     5581 ccattctgga aatgtggaaa attctgcagg cctgcgtcga agcggacgtg attgtgatta
     5641 cgcaggcggc gaataccggt ttgacgggcg gctcgacccc cgacggcaac gattacgacc
     5701 gcgacatcgt gattgtgaac accatgcgga tgaacatcat ccaaaccatc aacaacaacg
     5761 aacaagtcgt ctgcctgccc ggctcgaccc tgaaccagct tgaattgctg ctgaaacctt
     5821 tggggcgcga accgcattcg gttatcggct cttcctgcat cggtgcgtcc gtcttgggcg
     5881 gcgtgtgcaa caactccggc ggcgcattgg tgcagcgcgg ccctgcctac accgaaatgg
     5941 cactgttcgc ccaaatcaac gaagaaggca ggttggaact ggtcaaccac ttgggcatag
     6001 acttgggcga cacgcccgaa gaaatcctga ccaacctgca aggtcatcat tatcaaaaca
     6061 aagacatcaa acaagacgcg ggcaaagggc acgaccacgc ctattgcgaa cacgtccgcc
     6121 aagtggacga accgaccgcc gcgcgtttca atgccgaccc cgcccgccat tacgaagcct
     6181 cgggctgcgc gggcaaactg atggtttttg ccgtccgttt ggacaccttc ccgcaagaaa
     6241 aacaaaccgc cgtgttctac atcggcacga acgacatcaa cgagctgacc gacatccgcc
     6301 gcgccgcctt gggcgaattt gaaagcctgc ctgtttccgg cgaatacatc caccgccacg
     6361 ctttcgacat tgccgacgtg tacggcaaag acacgtttta cgtcatcaaa aaattcggta
     6421 cgcaccaact gccgaaatta tttgacttga aggcgcgcgt ggacaggttc ggcaaaaaag
     6481 ccagcttcct gcccaaacat ttttccgaca aggccatgca gttcgtcagc aaatacctgc
     6541 ccgaccacct gcccaaatcc atgcgcgatt accgcgacaa atacgaacac cacctgattc
     6601 tgaaaatggg cgggaaaggc gtggatgagg cgcgcgcgtt cttaaaagaa tattttgcac
     6661 accacagcgg cgcgtttttc gagtgcaacg ccgaagaaac ccaagccgcg atgctgcacc
     6721 gtttcgccgt cgcctccgcc gccatccgct accgcgccgt gcatgatgac gaagtggaag
     6781 acttggtcgc gctcgacatc gccctgcgcc gcgacgatcg cgactggttt gaaaaactgc
     6841 cgtcggaaat cgacaataaa atcatccata aactctacta tggacatttc atgtgccacg
     6901 ttttccatca ggattacatc atcaaaaaag gcaacgactg catggcgttg gaacacgaaa
     6961 tgctgcatct tttagaccaa cgcggcgcgc aatatcccgc cgaacacaac gtcggtcatt
     7021 tgtatgaagc caaacccgcg ctcaaacagt tttaccgcaa acttgacccg accaacagct
     7081 tcaacccggg cgtaggcaaa accagcaaaa agaaaaactg ggcggaataa gcgcgtccgc
     7141 tttgaaggca ggcaatgccg tctgaaggca aaacgccgtt cagacggcat tgtcgggttt
     7201 gaagttcccc atcgggtaat cgctttcaag ccgtatgccg gcacaggcgc gcgcgcctcc
     7261 caaatatagt ggattaaatt taaatcagga caaggcaacg aagccgcaga cagtacagat
     7321 agtacggcaa ggcgaggtaa cgccgtactg gtttaaattt aatccactat aaagtctgat
     7381 cctaccttcc caaagggcag ggtttcaacc gaaaaggaaa cacgatgaaa ccatacaaaa
     7441 tctacaccca tcccgccctg ccgccccaag ccgtcaaaca aggctggtcg tggccgggcc
     7501 ttttgttcgg cacgctgtgg gcgtgtttca aaagaatgtg gggtttgggg ctggggctga
     7561 cgggcgcgat attcgtgctg gcggtgtttg cccaactggt ttacggcgac acgcccgcca
     7621 cagactcggc gtttaacgta ttgggcttgg cggtttccgt ctggttcggc gcaaagggca
     7681 acagccttta tgcacgccac cttttatcgc gcggctatac cgaattgccc gaaacggttg
     7741 aggcagccaa tccccaagcc gcattggcgc aatatttcgg gcgcgggggc cgataagcgc
     7801 gttttctttt ccatcatgct gtctgaaaaa tgttcagacg gcatttctta taaccttttg
     7861 tttgcagcac ataaccaatc agtctttcct attccgttcc gaaatgcata ccatgcgccc
     7921 gtacccaata tttgcgaaca aggaaagaaa atggcacgtt taaccgtaca caccctcgaa
     7981 accgcccccg aagccgccaa agcgcgcgtc gaggcggtac ttcaaaacaa cggctttatc
     8041 cccaacctta tcggcgtatt atcaaacgcc cccgaagcct tggcgtttta ccaagaagtc
     8101 ggcaagctca acgccgccaa cagcctgacc gctggcgaag tcgaagtcat ccagatcatc
     8161 gccgcccgta ccaaccaatg cggtttttgc gtggcagggc acaccaaact cgcaaccctg
     8221 aaaaaactcc tgtccgaaca atccgtcaaa gccgcgcgcg ctttggcagc aggcgaattt
     8281 gacgatgcca aactcggcgc gctcgccgcc ttcacccaag ccgtaatggc gaaaaaaggc
     8341 gcggtatccg atgacgaact caaagcattt ttcgacgcgg gctacaacca gcagcaggca
     8401 gttgaagtcg tgatgggcgt agccttggca actttgtgca actacgtcaa caacctcgga
     8461 caaaccgaaa tcaaccccga attgcaggct tacgcttgat acggcaaacc gcccgaatat
     8521 cgggcggttt ttcaaaaaca ccccctcaaa caaaaacaag ccgcctaagc ggcagggaag
     8581 cagccgcgca tcaggcgcgc cccccgattt ccccgcaaac cttaaggaac aaagatgaac
     8641 gcccaaaccc tgattgccaa cgttgccgaa ttcgtcaaaa ccaagctcaa acccatagtg
     8701 gacgacatcg accgcaaagg atactacccc gaagcattta tgcgcgaact cggcgcaatc
     8761 ggtggattcg gcgcagtcgg tacagaagcc gaaggcggca acggcttggg tttggcaacg
     8821 caaatcgccg tattgcgcga aatcggcaaa gaatgcggcg caacctcttt cagtgcgtgg
     8881 tgtcaggcgg cttgcgcgtg gtatctgcac caaacgccca accgggccgt caaagacaaa
     8941 taccttgccg acatcctgca aggcaaagta ttggcgggta cgggcatgtc caataccgtc
     9001 aaacaccttg ccgacatcga aaaacacaac ctccaagccg agcgcgtgga gggcggctac
     9061 acagtcaacg gcgcgctgcc gtgggtgtcc aacatcggcg aagaccacat ttgggcgaat
     9121 accgcccaaa tcggcggcgg ctacgtcatg ttcatcacag gcggacaatg ggaaggcgta
     9181 agcctgcaaa actgccccga attttgcgcc ctcgaaggca cgcgcacctt cagcctgaac
     9241 ttcaaagacg tatttatccc cgacgaagac atcatcgccg cgcccgaaca gtttgccgac
     9301 tacatccaaa gcatcaaagc aggctttatc ctcctgcaaa tcggcatcgg cgcgggcgtg
     9361 atagacggca gcctcggcat catccgcctc gccaacgtcg tcaacgccga agtcaaccgc
     9421 tatctcgacg acggctacga cagcctcaaa acaaggctgg acggcgcgtg ggcagaaacc
     9481 gaacggctcg ccggattggc ttggagcggc acgcccgaca accttaccac cctcaagctg
     9541 cgcgaagccg ccgccgtact gacacttgcc gccgcccaat ccgccgccct gcattccggc
     9601 gcaaaaggct acctgatgcg gagtcccgcc caaaggcgcg tccgcgaagc aatgtttgtc
     9661 gccatcgtaa ctcccgcgat caaacacctc cgcaaagaaa tagcggcaat cgaagccgca
     9721 aaataaacag gcggaaaaaa ccgaaacccc catcgcgtca ttcccgcgaa agcgggaatc
     9781 cagcccccaa cgtaacagga atccatcgga aaaaacagaa accctcaaac cgtcattccc
     9841 gcaaaagcgg gaatccagcc ccccctaacg caacaggaat tcatcgaaaa aaccgaaacc
     9901 tccaaaccgt cattcccacg gaagtgggaa tccagtaatc gaaaaaccac aggaatctat
     9961 cgaaaaaaaa ccaaccccaa aaaaaccggg cggataccgc accattctcc caaaccctga
    10021 tttatagcgg atgaacaaaa atcagggcaa ggcggcgaag ccgcagacag tacaaatagt
    10081 acgggtgctt cagtacttta gagaatcgtt ctcttcgagc taaggcgagg caacgccgta
    10141 ccggtttttg ttaacccact atacaaaaaa caaggaaaca caaatggcgc aatatatgtg
    10201 cggcccctgc ggctggattt acgatgaaga acacggcgac cccgaacacg gcatcgcccc
    10261 cgggacaaag tttgaagaca tccccgacga ctggaaatgc cccgaatgcg gcgtgggcaa
    10321 agaagatttc tacctgttgg atttcgtgat atagcggcag ccgcaccctt agggaaaacc
    10381 ccgtcatccg gcgttccgcc tgcggcggca gggaacac
//
LOCUS       DAXHKX010000051        10207 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHKX010000051 DAXHKX010000000
VERSION     DAXHKX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.051"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(59..133)
                     /locus_tag="ACFMWZ_001033"
     tRNA            complement(59..133)
                     /locus_tag="ACFMWZ_001033"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Glu,seq:ttc)
     gene            complement(151..227)
                     /locus_tag="ACFMWZ_001034"
     tRNA            complement(151..227)
                     /locus_tag="ACFMWZ_001034"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Arg,seq:acg)
     gene            complement(337..2010)
                     /gene="recN"
                     /locus_tag="ACFMWZ_001035"
     CDS             complement(337..2010)
                     /gene="recN"
                     /locus_tag="ACFMWZ_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC5935009.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2194..2796)
                     /locus_tag="ACFMWZ_001036"
     CDS             complement(2194..2796)
                     /locus_tag="ACFMWZ_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC5935010.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLGLN
                     SLEELPPLTELGELVLPDLIEMQPTDEEEPETVPSDTLPN"
     gene            complement(2897..3838)
                     /gene="ylqF"
                     /locus_tag="ACFMWZ_001037"
     CDS             complement(2897..3838)
                     /gene="ylqF"
                     /locus_tag="ACFMWZ_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC5935011.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLGHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3919..4773)
                     /gene="rapZ"
                     /locus_tag="ACFMWZ_001038"
     CDS             complement(3919..4773)
                     /gene="rapZ"
                     /locus_tag="ACFMWZ_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC5935012.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4754..5716)
                     /gene="hprK"
                     /locus_tag="ACFMWZ_001039"
     CDS             complement(4754..5716)
                     /gene="hprK"
                     /locus_tag="ACFMWZ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC5935013.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNENRPD"
     gene            complement(5720..6169)
                     /gene="ptsN"
                     /locus_tag="ACFMWZ_001040"
     CDS             complement(5720..6169)
                     /gene="ptsN"
                     /locus_tag="ACFMWZ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC5935014.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6353..7243)
                     /gene="ubiA"
                     /locus_tag="ACFMWZ_001041"
     CDS             complement(6353..7243)
                     /gene="ubiA"
                     /locus_tag="ACFMWZ_001041"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC5935015.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPNLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRLRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7273..7749)
                     /locus_tag="ACFMWZ_001042"
     CDS             complement(7273..7749)
                     /locus_tag="ACFMWZ_001042"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC5935016.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRHFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7764..8411)
                     /gene="bioD"
                     /locus_tag="ACFMWZ_001043"
     CDS             complement(7764..8411)
                     /gene="bioD"
                     /locus_tag="ACFMWZ_001043"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC5935017.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8408..9709)
                     /gene="bioA"
                     /locus_tag="ACFMWZ_001044"
     CDS             complement(8408..9709)
                     /gene="bioA"
                     /locus_tag="ACFMWZ_001044"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC5935018.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            complement(9923..10162)
                     /locus_tag="ACFMWZ_001045"
     CDS             complement(9923..10162)
                     /locus_tag="ACFMWZ_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935019.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEELCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
BASE COUNT         2364 a         2795 c         2579 g         2469 t
ORIGIN      
        1 gagaaatgag aaatgagaaa tgagaaatgc ctccgatata atcggaggca ttttgaattg
       61 gcacgcccac ggggaatcga accccggtta ccgccgtgaa agggcgatgt cctaaccgct
      121 agacgatggg cgcatattgt attttcttac tggcgcaccc ggagcgattc gaacgcccga
      181 ccctctggtt cgtagccaga tactctatcc aactgagcta cgggtgcagc acaatcactt
      241 gttcaagtga ttaagaaagc gtgataatac ggatttatct gagttctgtc tattgtttta
      301 gacttttatt tttagttatt ttttaactga ttgattttaa aataaactat ttttcgacgc
      361 aagttgcagc aattctgccg catgttgccg cgtcgtatcg gtaatgactt ctccgcccaa
      421 catacgggca acctcttcga tccgttggat ttcatccaat atactgattt cgctgacggt
      481 ttgctctccc tcgctgtgct tgcgcacccg ccagtggttt tctccgcagg atgcgacttg
      541 gggaaggtgg gtaacggcaa gcacctgatg ttttctgccc aacgcacgta atgccttgcc
      601 gaccatttca gccactcccc ctccaatacc ggtatcgacc tcatcaaaaa tcagggtggg
      661 aacttgggta tattggctgg caacaacctg taaggcaagg ctgatacgcg ccaattcgcc
      721 gccggaggca actttattca gcggacgggg cggattgcct ttgttggcgg caacttgaaa
      781 ttgaacctgc tccaaaccgt gtgccgtcgg cgacgagggc aacaggacga tgtcgaaacg
      841 tgcacctttc atggcaaggt gttgcatatg ctcggtcgtt tcgccgctca aacgctctgc
      901 cgcctgatgg cgcatggcag aaaggatgtg ggcagcttcc tgatattcgg caaaattgtg
      961 ggcaacatta tgctcgagcg cgtccaaatc ggcggcagct tgcaggcttt gcaggcgttc
     1021 ttcgatttct gccaacttgg caggcaactc ttcaggctcg atccggtatt tccgcgccat
     1081 ccccatcagt tcgcccatac gctgttcctg tgccgccaat tcattggggt tgatgtcgct
     1141 gcgacctgcc acatcgcgca tattggcact gatttcgccc aattcggcct cgatgcttgc
     1201 caacatattc aggctctcgg caaagcgcgg ctcgatgttt tgcagattgg ccaatagttt
     1261 ttgacattga tagatatggc gttggatgcc gttgtcgccg tcaatcttgc ttccgacttc
     1321 ttcggcagcc tgcaacagct cggcagaatg ggcaaggctg tcgtggcttt ggctgagggc
     1381 ttcccattcg ccttgtttaa tgtccaactg attcaattcg ttaaactgcc attccagacg
     1441 ctcttgttcg agtgcaatgt tttctgcctg ttcctgcgct tcctgcaatg ctttttttga
     1501 gtttgcccag ttttgataaa gctgcctgac ggtttccgcc tgcgccctgc tgcccgcaaa
     1561 tgcgtccaac aattcgcgct gagcggcttc ctgattaagc gaatgatggg cgttttgccc
     1621 gtggatgtcg ataagctggc tgccgacggc tttgagttgc gccaaggtag cggcctgatt
     1681 gttgataaag ctgcggcttt tgcctttggc atcgataatg cggcggatac tgagttcttc
     1741 ttctccgtcg tttaaaagcc cctgttcata caattctgct tttaaaacag gtaaatggga
     1801 aatatcaaac aacgccgaca actgcgcttc ttttgcgccg ctgcggactt ggctgtaatc
     1861 ggctttatcg cccaacagca gaccaatcgc atccaaagta atggacttgc ccgcgccagt
     1921 ttctccggtc aatacggtaa agccgctttg aaaatccaga ttcagatttt cgacaatgac
     1981 aaaatcacgc aaagaaagtg ttagaagcat cgtgaggatt ccttagcaat tctaccgaca
     2041 aaagcaatat caactttaga tatacgaatt ataactaaga ttttttaaat taaaaataag
     2101 tttttcctta atttttctta gtgcttgttt ttatctgctt atttgttgca atgccgtctg
     2161 aagcaatgtg cgtttcagac ggcatttgga atttcagttg ggcagggtat cggacggtac
     2221 ggtttccggc tcttcttcat ccgtaggctg catttctatc aaatcgggca aaaccagttc
     2281 gcccagttcg gtcagcggcg gcagttcttc caagctgttc aaacccaaat cgctgaggaa
     2341 cgttgccgtt gtcgcccaca atgcgggttt tcccaatgtg tcccgatgtc cgatgacttc
     2401 aatccacccc cgatcctgca aggtctgcat cacgttctgc gacaccgcca cgccgcgtat
     2461 gccctcgatg tcgccgcgcg ttacgggctg ctggtaggcg ataatcgcca gtgtttccat
     2521 cacggcgcgg gagtagcgcg gcgcacgctg ttcttgcagg ctgcccagcc gctcgaatgc
     2581 cgtctgaaca atctgaaaac gccagccctc ttgcgtatgc accagttgca acgccctatc
     2641 ctgccaacgc gttttcaact gcgccaacac atcaatcagt ttgtcttgcg acaacggcgg
     2701 cacacacagt tcgcgcatag atttttcggt cagcggttcg gtttgggtca gcagtgcggc
     2761 ttcaatcagc gcgtcgggag aaattttgtc ggtcatacgg gtatccgtgc tgaaacggca
     2821 tgggtttgga tatgccgtct gaaatcggtt ggagtagaga gaagctgcct gaaaaatatt
     2881 tttcagacgg cattctttat gcttccgaag gcttctgccc tttacgctct gcttttctgg
     2941 cttcgcgtat ggctttgagt tcggcttctt tctgacgggc ttttttgagc caagtttccc
     3001 attggttcgg cgtttcgagg gtgattctgc cgattttgcc ttcacggaag tcggtaagga
     3061 tgttttcggc ggctttttgg tagttgatcc gtccgccgct gaggactgcg ccgcgttttt
     3121 tggctatcca ttcgagccaa acgttttcgt cccagtggct gctggggtct ttgtcggctt
     3181 ggtagcgttc ttgcaacata gggaggtagt ggcggcggag gtagtctaaa agttcgaggg
     3241 cgacttcttc ttcgtccaac gcgttgcgtc cgactgcgcc gccggcggca aggttgtagc
     3301 cgccttcttc gacgatgatt ttcggccaca gcattccggg ggtgtcgtag agccagaagt
     3361 catcggcgag gaagaggcgt tgttcggctt tggtgatgcc gggttcgttg ccggttttgg
     3421 cggatttttt gcctatcatg ccgttgatga gggtggactt gccaacgttg gggatgccgc
     3481 agatgaggac gcgcaggggt ttatctatgc cttgccggtg gggaatcatc gcgcggcagg
     3541 cttgggtgat tttgccgtgt gcgccggttt cggaggaatc gagggcaatg gcgcgcgtgt
     3601 cggggcggct gttgtagtgg ccaagccaga cctttgtgcg ttcggggtcg gcaagatctt
     3661 gtttatttag gattttaagt ttgggtttgc ctttggaaag ctgggcaagc agggggtttt
     3721 cgctggaggc gggcatacgc gcgtccagca tttcaatcac catatcaacg ctttttgcac
     3781 gctcggcgat ggcttttttc gccttgttca tatggccggg aaaccattgg attgccatgt
     3841 ctgttctttc ttttcaatat ttgaaatgcc gtctgaaacg gaggacgggg tttcagacgg
     3901 cataatggtt tgacggaatt agcggtctga caggttttgc gcctgtctgt gccgtatcag
     3961 caattcataa cgccctttca accttcgggc gagtttttcg acaatataga ccgaacggtg
     4021 ctgtcctccc gtgcaaccga tggcgacggt aacgtagctc ctgctttcat cctccaaacg
     4081 cggtaaccaa tgcgtaacaa acctttcgat gtcgtcaacc atttcctgca caagcggctg
     4141 tccgtccaaa taatcccaaa cgggcttgtc cataccggtg taaggcctca actcgggatc
     4201 gtaatacggg ttgggcaggc tgcgcatatc gaacataaaa tccgcgttgt tcggcacacc
     4261 gtatttgaac ccgaaggact ccaaaatcac cagcagcccg gtacgttcga ccttcagcca
     4321 ctgccggact gcatggcgga gctgttgggc attcatcttg gaagtgtcga tacaataggc
     4381 gatttcttta agcgggaaca gccattcccg ttctttcttt aagctttcca acaaggtcat
     4441 atcctgatta ctcagagggt ggcctcgcct ggtttcggaa aaccggcgga ccaacacgct
     4501 ttcttccgcc tcgacaaaca aaacttcaac cctgtgcccc agtccgcgca gagaggcaat
     4561 ctgttcccgc gcctgtccga tgtctatgcc ggaacgcaca tcgacgctga ccgccaattc
     4621 ggtttcgtcc gcacgctcga tatgatacga caccagcgcg ggcaacattt ccaaaggcaa
     4681 attgtccacg cagaaataac ccgaatcttc catttggcgc agtgcgacgg atttgcccga
     4741 accggacaac ccgctaatca ggacgatttt cattgtgttg ctcgttttct ttaagctgcg
     4801 tctgatgacg ttccaaaaat tcgcgcgtac tatccttacc gcgcaactga agaatataat
     4861 tgcgtaccgc cgcctcaacc aaaacggcga ggttgcgtcc gacggcgacg ggcagcgtaa
     4921 ccgaacggac gttgacgttg aggatggatt cggtttcggt gcggatgctc aaccggtcaa
     4981 gctgcttcat atactcgtcg tccgcctcga ccaaattgat aatgagttgc aggatttttt
     5041 tggggcggat ggaagtttcg ccgaaaatat ggcggatatt gagtatcccc aagccgcgta
     5101 cttccaaaaa atcgcgcagc ataggcgaac aacgtccttc caatgtttcc gggccgatgc
     5161 ggaacagctc gaccgcatcg tcggcaatca ggctgtggcc gcgcgaaatc agttccaatg
     5221 ccaattcgct cttacccaaa cccgaatgtc cggtaatcag cacgccgatt tcaaacacat
     5281 cgagaaatac gccgtgtttg acggacgatg ccgccagggt gcgttgcagg taaatccgca
     5341 acacatccat cagatagggg ctttcgagtt tggaagtcag cagtggaata tcgtttttat
     5401 ggcagtagtc gcgcagtccc ggggaaaccg gcaagccgtt tgccacaatg accaaagaca
     5461 tagaaatgtc gaacaggtcg ccgaactgat aacccgtttc ccccgattcg agacggttca
     5521 gatattccga ctccgccaaa ccgaccactt ggatttggtt ggggtggata aaattcaggt
     5581 gtccgaccag ggcgaggacg ggcttgtccg cctctacgcc gatacggttg tccgcacccg
     5641 aattgccggc ggcccaggca agttgcaact tgtattggtt gtcatcaaac agtcggcgga
     5701 cggagatact gggcatattt tattcttcag tcaggatggc gcgggcttct tccgcagaag
     5761 aaaccgtcat cagcgattct ctgatgcttt tttgggaaaa cttgccggcc agtttggaca
     5821 atacttccaa atgctcaccc gtcgcatttt ccggaaccag caagataaaa accagggaaa
     5881 ccggtttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg aacgcgcccg
     5941 tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc cccaaaccgg
     6001 tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg gacaatgagg
     6061 attcgcgttc caaaagcagg cccgcttcct caaacagcct ttttttactg cccacctcca
     6121 tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata agcttctctt
     6181 tcagacatcg caaaacagga agattgtacc gactgccggg gcaaatctca atcccgcata
     6241 cggtacgggc tgacataaca cagcgtttta aaaacatatt ttaacgctct tcggcacaga
     6301 tagaaatgcc gtccaaagcg gtttacggct cttcagacgg cattgccctg ccttatttcg
     6361 cgaaaaacgt atgggcaaaa atggcggtaa accacaccca accaattctg ttgtttgcca
     6421 aaaacgtttc aaaacagatt tgccggaggc ggcttttgat ggcggcatat tggcggtatt
     6481 gcagcagcag gacgatgggg attgccgtcc aatatgccca tgccgcaccg ataaccgcac
     6541 ccaatactgc catcagcagg gtaaagccgc catgacacag cataacggcg gcgatgtcgt
     6601 aacgcccgaa cgtgacggcg gaggttttga tgccgatttt caaatcgtct tctttgtccg
     6661 ccattgcata aaccgtgtca tacgccagag tccataacac attggcggca aagagtatcc
     6721 acgcttgagg cggcacgttt ccggcaacgg cggcaaacgc catcgggata ccgaaggaaa
     6781 aggcaagccc gagatagagt tggggaatcg gaaaaaaacg tttggtaaac gggtaagtca
     6841 gcgcaagaaa cagcgcgggc aggctcatca gccatgtcag atgattcagc ggaatcaggc
     6901 acaatgcggc aagcaggcac aaaaatgccg tcagcagcag cgcttctttt ttcttgaccc
     6961 ggccctgtgc gaacggacgg tttttagtgc gctcgacagc cccgtcaaaa tcgcggtcgg
     7021 caaaatcgtt gatgacgcaa ccggcactgc gcattaaaaa cgtaccgatt gtaaacgccg
     7081 ccaataccgc caaattggga atgccgtctg aagccagcca caatgcccag taggtcggcc
     7141 acagcaaaag cagcgtccca atgggcttgt ccgcccgcat caggcgcagg tacacatcca
     7201 aacgatcgga cagtcgtaaa aataaagggg atttaggatt catattgccg cacaacttga
     7261 aaaaacggta ttttatccga taaagcgttt cagttcgggc agaaaatact cggtcagcag
     7321 catttcctcg ccgtgacggg aaaaccgaga acgccgcgcg gcaaagtgcc gtccgcatcc
     7381 ttcgccggaa acggcaaact caaacgccga acgcgcccct tccaaatcgg cttgaaacag
     7441 acgctcgccc aaagggcgcg tgccgcagtc caaaatgttt tgccaaaacg ccgaacctat
     7501 ccggcactcg ctccttgccg cgacaacagg gataccgtcc agcttcaaca aaacttcgcg
     7561 caccagcctc cctccgcatt ccgtctccaa ttcgcccagt ttcagcagtt ccaccgaaaa
     7621 tgtatgcggc aaggcgcgca atgcggcggt cagcgaccgg gctttcaata accggctcat
     7681 cggcaggtcg atgccgtctg aaacggcggc gggcaagtcg ggcagccatt tcccaaatag
     7741 gtgttccata tttttcccaa tctttatacc gcgtctgttt ttgccaactc catccattcc
     7801 gcttcggaaa aatcggcttt cagacggcat ttcaagtagc tcaggctgtc ttgggcgatg
     7861 tgtgcgtcgc ggctgtcgtg gatgtggttg aacacgaggc tgtacaagcg aatgccgtat
     7921 tgtttgagcg ctgcgaaact gagtaaagtg tggttgatac tgccaagccg tccgctggta
     7981 acgaggatga cgggatagcc ttgctgacgg atataatcaa tggttaacag gttttccgtc
     8041 agcggaacca tcaatccgcc cgcgccttcg accaaaacga cttcgtactg cgccgccaat
     8101 tcttgtgtgg cggtgcggat tttgtccaag tccaaagccc tgccgtcaag acgggcagcg
     8161 agatgaggcg aagctggata actgaagatt tcaggcatgg tcagcccttg tttgtcggct
     8221 tcctgcatgg gtttacccat gattttacga tggacggcga tgtcgtcggc aatgttttgg
     8281 caaccggttt gcacgggctt ttgcgtaatc acgcttttgc cctgctgcaa caattgtttt
     8341 gccaacacgc cggtggcgac ggttttgccg atgtccgtgt ctatgccgct gacgaagtaa
     8401 acgcctttca tttgctgtgt tccttcaaga tttgcacggt tttgtcggca agtttggtca
     8461 aaacgccgtc tgaaatgatg taaggcggca tcagatacac caacctgccg aacgggcgca
     8521 cccaaatgcc ctgcgccacg caatccgctt gaaaacgcgc catatccacg cctttttcca
     8581 actcgatcac gccgatggcg cccaaaacgc gcacgtcttt cacaccacga atgtcccacg
     8641 cggttttcag acggcatttt aagatgcttt cgatgcggcg gatatttgcc tgccaatctt
     8701 gggacaaaag cagtttgacc gaagcgcagg caacggcaca cgccagcggg ttcgccataa
     8761 atgtcgggcc gtgcatgaac acgcccgctt ctccgcgcga aatcgtttcg gtaacttttt
     8821 gcgaagtgat tgctgccgcc agcgtcatat agccgccgct caaacccttg ccgatgcaca
     8881 taatatccgg cacgacctcc gcgtgttcgc aggcaaacat cttgcccgtg cgcccgaatc
     8941 cggtggcgat ttcatcaaaa atcaaaacga tatcaaactc gtcgcacaaa tcgcgcaatc
     9001 cgcgaagata ctgcggatga taaaaataca taccgcccgc gccctgcacg accggttcca
     9061 aaataaaggc ggcaatgtcc gtatgatgcg cttcaaacaa ggcgcggaca ggctgcaaat
     9121 ccgcctcgtc ccattcatcg tcgaaacggc ttgtcaggtt atcgacaaaa taacgctgcg
     9181 gcaacgcgct gccgaaaata tggtgcatcc ccgtttccgg atcgcagacg gacatcgcgt
     9241 tccaagtatc gccgtgatac ccgcgccgca ccgtcgccat attttgtttt gcacgcaaac
     9301 cccgcgcctg ctggtattgc accgccatct tcaacgcgac ttccaccgaa accgaacccg
     9361 aatccgcata aaaaatacga tccagcccct gcggcaaaat cccgaccaac aatttgccca
     9421 gctccaccgc cggctcatgc gtcaaaccgc cgaacatcac atgcgacatc tgtttcatct
     9481 gcgtctcaac cgcctgattc aaaacgggat gattgtaacc gtgtatcgca caccaccaag
     9541 acgacatccc gtcaatcagc cgcgtgccgt ccgccaattc gataaacacc ccttctgcac
     9601 gtttgacagg ataaacggga agcggatcgg tcatggaagt atagggatgg agcagatggg
     9661 tacggtcgaa atcaagcaat gatgatgtgt gttgatgttc agacggcata agtttctctc
     9721 ttttcttatt gctgcattca aacgcaaaac gcgtattcta ctccgacaga ccgcttccca
     9781 cacctttcca tccgtttcgg gcgtaaaacc acgcaacaaa tcgtccgcag tataagcgca
     9841 caccgtttcg cattccccaa gcccgattgg aatcagacga cccaacgccc aatattgttt
     9901 cacacagcct ccgcccaacg cttcaatcag tcatagacaa aacggcaatc catacgatac
     9961 agatcatagc aacagccatc gcaacagcgt tagcaaaatc aggggactcc gacataggcg
    10021 catagcatct accgatgcac agctcctcat tcggctctat gaataccata cccatcacaa
    10081 aatccaccgc caaaaccagg cacggcttct tatacttatg atagatttcc accatcctgc
    10141 cccatatatc ccaaacattc ataccgcata tcccgcaggc aacaaattcc gattgaaggt
    10201 tacagcc
//
LOCUS       DAXHKX010000052        10188 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHKX010000052 DAXHKX010000000
VERSION     DAXHKX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.052"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            1..153
                     /locus_tag="ACFMWZ_001046"
                     /pseudo
     CDS             1..153
                     /locus_tag="ACFMWZ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            528..1001
                     /locus_tag="ACFMWZ_001047"
     CDS             528..1001
                     /locus_tag="ACFMWZ_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC5935020.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNIFELKPVLEDLASEMRDYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENISRLYFNWGKYLSNKSRSG"
     gene            1130..1804
                     /locus_tag="ACFMWZ_001048"
     CDS             1130..1804
                     /locus_tag="ACFMWZ_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC5935021.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNACPIKPKPTNHAQQRKEEAKNDSH
                     RSVGDSNRVVREGKQYLDSDTGNHVYVKGDKVVILTPDGRQVTQFKNSKANTSKRVKN
                     GKWTPK"
     gene            1782..2063
                     /locus_tag="ACFMWZ_001049"
     CDS             1782..2063
                     /locus_tag="ACFMWZ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935022.1"
                     /translation="MGNGHQNNRSPLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
     gene            2442..3575
                     /locus_tag="ACFMWZ_001050"
     CDS             2442..3575
                     /locus_tag="ACFMWZ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935023.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLENLEKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(3662..3937)
                     /locus_tag="ACFMWZ_001051"
     CDS             complement(3662..3937)
                     /locus_tag="ACFMWZ_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935024.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(3954..4727)
                     /locus_tag="ACFMWZ_001052"
     CDS             complement(3954..4727)
                     /locus_tag="ACFMWZ_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC5935025.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVKEQTSFNNPEPMTGFE
                     HTVTFDFQGIKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFT
                     VQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(5114..5389)
                     /locus_tag="ACFMWZ_001053"
     CDS             complement(5114..5389)
                     /locus_tag="ACFMWZ_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC5935026.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(5386..6669)
                     /locus_tag="ACFMWZ_001054"
     CDS             complement(5386..6669)
                     /locus_tag="ACFMWZ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC5935027.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRVPILGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(6820..8238)
                     /gene="glnA"
                     /locus_tag="ACFMWZ_001055"
     CDS             complement(6820..8238)
                     /gene="glnA"
                     /locus_tag="ACFMWZ_001055"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC5935028.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGETAYFGPEPEFFVFDGIEFETDMHKTRYEIRSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            8322..8471
                     /locus_tag="ACFMWZ_001056"
     CDS             8322..8471
                     /locus_tag="ACFMWZ_001056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935029.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLRFVCSKAEYPPFCPNAD
                     KTDAA"
     gene            8549..9358
                     /gene="aroE"
                     /locus_tag="ACFMWZ_001057"
     CDS             8549..9358
                     /gene="aroE"
                     /locus_tag="ACFMWZ_001057"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC5935030.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSREIFRNCRLAYDMVYGDA
                     AQAFLNFAQSNGAAEVSDGLGMLVGQAAASYHIWRGFTPDIRPIIEYMKAL"
     gene            9361..10062
                     /gene="mtgA"
                     /locus_tag="ACFMWZ_001058"
     CDS             9361..10062
                     /gene="mtgA"
                     /locus_tag="ACFMWZ_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC5935031.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTACVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
BASE COUNT         2787 a         2439 c         2468 g         2494 t
ORIGIN      
        1 aatacgcgcg aagccgttta cggatggata taggacaacc ccaatgccgc cgaaaccgtc
       61 gaagccctgg tcaacatcct gccgttttcc aaagtcaaag gattggtgaa ggcggcgaaa
      121 ctggggaggg cggcaaaacc ggggagggct gcgaaactag ggaaggcaaa ggttagcgag
      181 gattttgctg ttgccagtca ggcaagatat acttcgcaaa cggcagaaaa tataattgca
      241 gccgatagaa taggaagtgg attaaaagct gatacgtcac atcgtgcagc cagctttcta
      301 tctcaagaac aattgggagc aggaagaaca ttctcaatta ctggcggcga tggtattcaa
      361 agaactttat tgcagacaaa aggttctttg aacggtcaaa gcggtatata tgaatatatc
      421 ctcgattcaa ataacaaagt gactcatcaa agattcatta aaggaggtga aattacagga
      481 caaccaaacc aacgcgtgaa aactaaagta cgcaaccggg ggaaaaaatg aaccttgatt
      541 taaccgctca aaaagtccgc ctttcttgga aggatattct gtgggggtat gagaataaat
      601 acttgggttg ggctgatgtg gcagcttatg cccgaaaaat gacgctttca gatcatgatg
      661 aacgcgtgtt caagctatct ttaatcaaca aatccaatat ttttgaatta aagcctgttc
      721 tagaagattt ggcttcggaa atgagggatt attcccctaa aaattggctg tacatcctcc
      781 taaatgacgt attccataga aaagaagaat ttgaggatcc tctgggggaa gttgaaaaaa
      841 tttatgcgga ttttgattac ccggaagaaa tagaatcatt tgtcaggtat atgccgccca
      901 aagacggcta tatcccttct aatcatagtt atgaagaaaa tatttctcgg ttatatttta
      961 attggggaaa atatttgagc aacaagagta ggtcgggtta aaaattcaac aatcaaaagg
     1021 tcgtctgaaa ctgcttttaa ttttcaggcg accttcccct tattttaaac aggatattgg
     1081 gaactaagtt cttcaaaaat cctacacctg ctccttccac ggcagcacct tggtcaaaac
     1141 ggcagacggc tacaaagcca ttgcccgtat ccgaaccggc gaccgcgtct tcgccaagga
     1201 cgaggcaagc ggaaaaacgg gatacaaacc cgttaccgcc cgatacggca atccgtatca
     1261 agaaaccgtt tacattgaaa tttcagacgg catcggcaac aaccaaaccc tgatttccaa
     1321 ccgcatccat ccgttttaca gtcaaggaaa atggatacag gcaggtcgtc tgaaaaaagg
     1381 cgacaccctg ctttccgaaa gcggtgcaaa acagacagtt caaaacatta ccctcaaaca
     1441 gcagccgctc aaagcctaca atctgaccgt tgccgattgg catacttact tcgttaaggg
     1501 taatcaggcg gaaacggaag gggtttgggt tcataatgcg tgtccgatca aaccaaaacc
     1561 aaccaatcat gcccaacaaa gaaaagaaga agctaaaaac gattctcatc gaagtgtggg
     1621 agattccaat cgtgtcgttc gcgaaggaaa gcaatattta gattccgaca caggaaacca
     1681 tgtttatgta aaaggagata aagtggttat tctaactcct gatggaagac aggtaactca
     1741 atttaagaac tcgaaagcca atacgtcaaa aagggtaaaa aatgggaaat ggacaccaaa
     1801 ataatcgatc tcccttagaa aaaagaattt tttatctgga gcattcgggg cagtatctga
     1861 tgatttgcgc cctatcagat tactcccaaa ataaacatac tgtcgttatg gcgaattttc
     1921 tctatccaaa tgaaaaaatg gattggagaa atttagatga tttgttcaat gagttggttt
     1981 tagaggaatt acagtcttca tttatggatt ggtatccgac tattgaagag gcaattagcc
     2041 atcatttaga agacttttcg taacaagcat aaattaaagg ttgtctgaaa ctatgttcga
     2101 ttttcgacga cattttcctc attggagggg gcggcaattt atggaaaata gatttgaaaa
     2161 aatcgttaag gatattttta gttttaccca atttgttccg aagcagaaaa tgagattccc
     2221 accccatcct cccgttatga cgactgttgg tgggaattgg aaatcttgaa tgcctctgca
     2281 tttgaattag tcaaaaaatt gaatcaaagc aaacaagagt aacaagctgc agcctgcata
     2341 ggggttttta aagtacggca gcaaccgtag attaaattaa aaatcaaaca atcaaagctc
     2401 gtctgatata atcgatatat tctgttaaac gaaaaataaa tatgtttaaa aaattcaaac
     2461 cggtactgtt gtcatttttt gcacttgtat ttgccttttg gctgggaacg ggtattgcct
     2521 atgagattaa tccgcgttgg tttttgagcg atacggcaac tgaaatacct gaaaatccga
     2581 atgcttttgt ggcgaaactt gcccgcctgt tccgaaatgc cgacagggcg gttgtcatcg
     2641 tgaaggaatc gatgaggacg gaggaaagtc ttgccggagc tgtggatgac ggtccgttgc
     2701 agtcggagaa ggattatctc gcgctcgcta tccggctcag tcgtttgaaa gaaaaggcga
     2761 aatggtttca cgtaacggag caggaacatg ggaaagaggt ttggctggat taccatatcg
     2821 gcgagggcgg tttggttgcg gtttcgcttt cgcaacgctc gccggaagcg tttgttaatg
     2881 ccgaatatct gtatcggaac gatcgtccgt tttctgtaaa tgtgtacggc ggaacggctc
     2941 acggagaaaa ttatgaaacg acaggagaat atcgggttgt ttggcaaccg gacggttcgg
     3001 tatttgatgc ggcagggcgc gggaaaatcg gggaagatgt ttatgagcat tgcctcgggt
     3061 gttatcagat ggcccaggta tatttggcga aataccggga tgtcgcgaat gatgagcaga
     3121 aggtttggga cttccgcgaa gagagcaacc ggattgcatc ggactcgcgc gattatgtgt
     3181 tttatcagaa tatgcgggaa ttgatgcccc gagggatgaa ggcaaacagt cttgtggtcg
     3241 gctatgatgc ggatggtctg ccgcagaaag tctattggag tttcgacaat ggaaaaaaac
     3301 gccagagttt cgaatattat ttgaaaaacg gaaatctttt tattgcacaa tcttcgacgg
     3361 tagcattgaa agcggatggc gtaacggcgg atatacagac ctatcatgcg caacagacgt
     3421 ggtatttgga tggcgggcgg attatccgcg aagagaaaca gggagacaga ctgcctgatt
     3481 ttcctttgaa cttggaaaat ttggaaaaag aggtgcgccg ttatgcagag gctgcggcga
     3541 gacgttcggg cggcaggcgc gacctttctc actgaacgca aatgccgtct gaaaccggtt
     3601 ttcagacggc attggagagt caaacgcttt ttgcaatgcc gtatatgccg aatccaattc
     3661 gctacaaatt gaaaaccaca atccaaaaaa cagaaataca cattaaacgg atctttttac
     3721 cgaccgtatc atcccatagg ttttccacac cttttttcat ccataaattc aaaccattta
     3781 agtttgcatc cttaccgaca attgcagcag catcatccgc ataacggtca cccaaattag
     3841 ccaaataacg ccaaccgcca taggtatcgc cacggaaagc ataaccacgc aaggtttgga
     3901 tttgttctgg tgttaaaaca acttcaccct cattcataat aagatccttt aaattatttt
     3961 ttacttgaat tgtttacaaa actctgtacc aattgctcta cttgttggtg ataacctacc
     4021 aactcctgct gtgtgagctg ctgcttttcc cgaataaacg ggtaatactg taccgtgaaa
     4081 ccgttaatac aggaacctac gctagtgcta taattatctt cttcagcctg gtttactcga
     4141 ttattccaat gagtaataat cgccggattt tctccttttt taatatagta aaattcatga
     4201 aaatatggat tttcaggaat atgtctttta tcttcataag cttcatattc aggataggca
     4261 gatggcattt ttttaacccc gtaagtaagc aatctttctc ccatagtcgt acttaaacta
     4321 cacgcatctt ttcggataac aatatcgtcc acgctatcca aaccgtcgcg tagaaattgg
     4381 ataaagtgtg ctttgttttg ctggttgtat ggttcgagca cccagccttg gtcggttttt
     4441 ttgtaataga cgctaatctc tatcaaatta atggagtaag catcctgccc gggcgtgtcg
     4501 gaaagccatt ttgtggcatt gtcttgcgta taccgtgcaa gatagccata ggggataacc
     4561 attttgatgc cctgaaaatc aaatgtaacc gtatgttcaa atcctgtcat tggctcggga
     4621 ttgttgaaac tggtttgttc tttaacaggt tcacatgagg tcatagtaaa aacactccca
     4681 ataaacaaaa taaaaagttg ataaatggta gtagcatatg gtctcataat ttcaagctta
     4741 gaaattagtt aaagaatata ttatggtaga tactaactct ttaagttgac atattgtgcg
     4801 atatcgaggg ctgtaccgta ttttcccatc gtatcttccc cgttctttat atgagcggca
     4861 ggcattctca taaaagaagg gataccgata aggaaaaata cctcggtata aaaaatatac
     4921 tgattgatta atacaaatcc aacaaacagt tcatcgtatt cttttctgtt ggcgtataaa
     4981 aaatactgat atatgccttc actttcatag ggcgacgctt tatgtaccgt cctgtgtgtt
     5041 gaaacgtcgg ttttccttac cgcctgcccc gcttttccct gattgctgcc aactgccgtc
     5101 cgaacttgaa ccgtcaggtt tcagacggca ttttttctgc ctgctcccac aattgccgtt
     5161 tttgtgcggc ggtcagtttt ttgacggcga tttcctgcct gagtgccgtg cctttatcca
     5221 tcccgcctgc aacgatacgc atcgccaccg gtttgaatac gcgggtatat ttcgcgcctt
     5281 taccggttgt gtgggcggca agccgctgtt gcggattcgg gctgatgccg caatagaacg
     5341 cgctgttttc acacaatatc aggtaaacgc tccagtttga cgcgttcatc tgcctgcatc
     5401 ctgagttttc tccccgttcc aaggcgcaac tttcagcagc agcagcatac ccggtagggc
     5461 gagtgcgaaa cacagttgga aaaacggtac ataaccgagc cattcaatca gataaccggc
     5521 aaaggaattg atgaccgtgc gcgggacggc ggacaggctg gtaaacagcg caagctgcgt
     5581 tgcggtaaat gcgggattgg tttcacgcgc catatacgat acgaacgccg ccgtccccaa
     5641 acccacgccg actgcctctg cgccgataac tgccgccagc atcagcctct cgcctgtgcc
     5701 gaccgtgtcg aaatgtccga accctgccag ccatacaaac cccaaaacgg ttacagcctg
     5761 caccgcgcca aatatccaca aggctttgtt tacgccgatt ttcagcatcc acacgccgcc
     5821 caagatacct gccgccactg ccggccacag tcctgcgttt ttggcaatca aaccgatgtc
     5881 ggtcttgctg aaacccatat ccagataaaa cggcgttgcc aacgcggttg ccatactgtc
     5941 gccgagtttg taaaggaaga taaacagcag cacgcataca gccgaagcga tgcccttgcg
     6001 tgtaaaaaat tctttaaacg gctctaccac tgtctgcttc aacgttttag gaacggaagg
     6061 cggcaacacg ggttcgcgcg cgagaaacag cgtcatcaga aggccgggca gcataaataa
     6121 tgaagtgata acaaatactt ctgaccacgg catcctgtct gccaacacca aactcaatga
     6181 accgggaatc agggcggcaa tccggtaggc gttcacatgg actgagttgc ccaaacccaa
     6241 ttcctcgtcc gacaaaatct cgcgcctgaa cgcatccaat acaatatcct gactggcgga
     6301 aaaaaaagcg acaagcaccg acaagccggc aatcaacggc agatgattcc gggggtttaa
     6361 aaaggcatag gccgccaaag ccgccagcaa ccctgcctgc gtcaacagca tccacccgcg
     6421 ccgccggccc aaaatgggaa ccctgaccgc gtccatcagc ggagaccaca aaaatttcca
     6481 agtaaacggc agaccgatca gcgccatcag cccgatgctc ttcaaatcca cctgctcgct
     6541 gcgcaaccat gccggaatca ggttaatcag aaagtacagc ggcagccccg aggtaaaccc
     6601 cgtaaaaata cagacaagca tatttcggga aaagatttgc ccgataaaac ctgatttcga
     6661 tacagtcatg gttcaaacag ccaagaaata aaagcctatt gtagcaaata tcgcccaacc
     6721 gctgaaaaac accgaccctg ctccgccgtt ttgccgttca aatgcaaaat gccgtctgaa
     6781 actttgttca gacgacattt ctactgaaac ctgagagttt tacaggctgt aatacatttc
     6841 aaattccagc ggatgcggtg ccatgcggat acggcggaca tcttcttctt tgaaagcgat
     6901 gtagctgtcg atccagtctt tgctgaatac gccgccgcgc aggaggaatt cgtggtcggc
     6961 tttgagggcg gccagtgctt cttccaaaga agcgcaaacg gtcggcacca atgcatcttc
     7021 ttccggcggc agatcgtaca ggtttttatc ggcagggtcg cccggatgga ttttgttttg
     7081 aatgccgtcc aaacccgcca tcaacagggc ggcaaatgcc aaatacgggt tggcggtcgg
     7141 atcggggaaa cgcgcttcga tgcggcgcgc cttgctgctg ttcacggacg gaatgcggat
     7201 ggaagcggaa cggtttttgg cggagtatgc cagtttggtc ggcgcttcaa agtgcggcac
     7261 gaggcgtttg taggagttgg tggacggatt ggtaatcgcg ttcaaggctt tggcgtgttt
     7321 gatgatgccg ccgatgtagt agagcgcggt gtcgctcaag ccggcatagc cgtcgcctgc
     7381 gaacaggttt tgaccgtctt tccaaatgga ttggtgaacg tgcataccgc tgccgttgtc
     7441 gcccataatg ggtttgggca tgaaagtggc ggttttgccg aagttgtggg caacgttttg
     7501 aatcacatat ttcatgtctt gggtttggtc ggcgcgtttg accaaagtag caaagcgcgt
     7561 gccgatttcc atttggctgc cggtgccgac ttcgctgtgg tgcacttcca cttcaatacc
     7621 gagttcttcc aaaatgttta ccatcgccga acgcaaatcc tgaccgcagt caatcggtgc
     7681 aacaggtgcg taaccgcctt tgacggtcgg gcggtggccg gtgttttgac cgtccatatg
     7741 cagaccgctt gcccacgcgc cgctttcgga cctgatttcg taacgggttt tgtgcatatc
     7801 ggtttcaaat tctatgccgt cgaatacgaa aaactcgggt tcgggaccga aataggcggt
     7861 ctcgccgata ccggaagatt tcaaataggc ttcggctcgg cgggcgatgg agcgcgggtc
     7921 gcggtcgtaa ccctgaccgt cggcgggatc gataacgtcg caagtcaaca caacagtcgc
     7981 atcatcataa aaaggatcga cgaaggctgt agacgcatcg ggacgcaact gcatatcgga
     8041 agcctgaatg cctttccagc cgccgataga cgaaccgtca aacgcctgac cgttttcgaa
     8101 ccactcttcg gggtcttcca acacgatgcg cgcaggcacg gtaaagtggt gctgcttgcc
     8161 tttggtatcg gtaaagcgca aatcgacaaa gcgggcttcg ctttcttcaa tcaattttac
     8221 ggcgttttta atagacattt ttcagctcct ggaaaaggga aatagcaaaa tatacggaac
     8281 gcggcagatt agcccaaagc cagcatgatt ctaccacgtc catgccgtct gaaactcgcc
     8341 ctgcgtcaaa tttcaagctg gaaaatatag tttcagacgg caagattgtc aacaatatgc
     8401 ttcggtttgt ctgttcaaaa gcagaatacc cgcccttttg tccgaatgcc gacaaaacag
     8461 atgccgcctg aaaacagttt agacggcatc gttcccatct tcgcgccgcc gttataaaat
     8521 gaccgccccg ccttgatgga atatgactat gcacgccctc ccccgctacg ccgtttttgg
     8581 caaccccgtc gcccacagca agtcgccgca gattcatcaa caatttgccc ttcaggaagg
     8641 cgttgacatt gaatacgaac gcatttgcgc cgacatcggc ggtttcgcgc aggcggtttc
     8701 gacatttttt gaaacaggcg gttgcggggc aaacgttacc gtaccgttca agcaggaagc
     8761 gtttcatctg gcggacgagc attctgaacg cgcattggct gccggcgcgg tcaacacgct
     8821 gatttttctg aaaaacggaa aactgcgcgg cgacaatacc gacggtatcg gtttggccaa
     8881 cgacatcacg caggtcaaaa acattgccat cgaaggcaaa accatcttgc ttttgggcgc
     8941 gggcggcgcg gtgcgcggcg tgattcctgt tttgaaagaa caccgtcctg cccgtatcgt
     9001 cattgccaac cgcacccacg ccaaagccga agaattggcg cggcttttcg gcattgaagc
     9061 cgtcccgatg gcggatgtga acggcggttt tgatatcatc atcaacggca cgtccggcgg
     9121 cttgagcggt cagcttcctg ccgtcagccg tgaaattttt cgcaactgcc gccttgccta
     9181 cgatatggtt tacggcgacg cggcgcaggc gtttttgaac tttgcccaaa gcaacggcgc
     9241 ggccgaagtt tcagacggcc tgggcatgct ggtcggtcag gctgccgcct cctaccatat
     9301 ttggcgcggg tttacgcctg atatccgccc cattatcgaa tacatgaaag ccctgtaacc
     9361 atgttccgca tcatcaaatg gctgattgcc ctgcccgtcg gcatctttat ctttttcaat
     9421 gcctatgtgt acggcaacat cattacctac cgcgccgtcg cgccccatca gactgccttt
     9481 atgtcgatgc ggatgaagca gtttgaacag gaaggtcgcg atgtcgcact ggattaccgc
     9541 tggatgccct acaaccgcat ctcggtcaac ctgaaaaaag ccctgattgc ttccgaagat
     9601 gcccgtttcg ccgggcacgg cggcttcgat tggggcggca ttcaaaacgc catcaggcgc
     9661 aaccggaaca gcggcaaagt gaaggcgggc ggctcgacca tcagccagca gcttgccaaa
     9721 aacctcttcc tcaacgaaag ccgcagctat atccgcaaag gcgaagaagc ggcgattacc
     9781 gcgatgatgg aagccgttac cgacaaagac aggatttttg aactgtattt aaactcaatc
     9841 gaatggcact acggcgtttt cggcgcggaa gccgcgtccc ggtattttta tcaaataccc
     9901 gccgccaagc tgaccaaaca gcaggcggca aaactgacgg cgtgcgtccc cgccccgctc
     9961 tactacgccg accatccgaa aagcaaacgg ctccgcaaca aaaccaatat cgtgctcaga
    10021 cgcatgggtt cggcagagtt gcctgaaagc gacacggact gattgtttca gatatggaaa
    10081 tgccgtctga actgcggttc ggacggcata tgttttctgg tttttatagt ggattaacaa
    10141 aaatcaggac aaggcgacga agccgcagac agtacaaata gcacggaa
//
LOCUS       DAXHKX010000053        10188 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHKX010000053 DAXHKX010000000
VERSION     DAXHKX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.053"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(418..2163)
                     /locus_tag="ACFMWZ_001059"
     CDS             complement(418..2163)
                     /locus_tag="ACFMWZ_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFC5935032.1"
                     /translation="MCKEVKPNFTEEAAPLGDMAEFAAPFAQPRYDVDGAGVWYIGVK
                     TDREGNTQEAAPVKLSSPIDIIGRGTDNDGAYYRVIRWQDANTRRTKTAAIPQSEIGT
                     VQGGQRLQSYGLAVLSGRVKRERLSDYLQTQGSGDIYTITDRAGWHGNAYILPNGETI
                     NAEGANILYNGDTSQREGYTESGSLEEWRQEAARYAEGNSRLCLALGLSLAAPFLALL
                     HEEGGGVHLAGSSSKGKTTAANLALSVWGSYEATKSNWDTTALGLQNAALARNDGFLA
                     LDEIGQTADPRRIPQMVYSVINGISKTQGAKDGGNRKQKTWRNLILSTGETNPESLIG
                     DRAQWRAGNRARLPDIQAEAKHGIYDTLHGFTDGAKLSEHINRAAAKKYGTAGRALIR
                     QILSDGKEAAAETIEATRARYLEALPPMEGQARRIVRRFTVLEYAAPITGLKAGGAAV
                     MQCFHEWLEENGTGDRDAEAICSQLDDFIAQYADFPRFLSWSERDAPYTMTGGKGKGH
                     AGFKVQAGGNDEFYILPLVFREEIAQSFPIHTVCKVLSGKGRLKPSKTGYQHQIKVNK
                     KPIRHYLIIEPLELD"
     gene            complement(2282..3334)
                     /locus_tag="ACFMWZ_001060"
     CDS             complement(2282..3334)
                     /locus_tag="ACFMWZ_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFC5935033.1"
                     /translation="MKPTYQDIKAAARYRWQEIHAAIGIAPKFLRNKHQPCPACGGED
                     RFRYDDKDGKGTFICTHWENGAGDGFGLVMHFLGCDFQTALKQVSGIFGMDETNPLPI
                     PPTRPQPQPRPEKDHIGKLAALWDEAELLTPDCPVVRYWAGRGLTVARLPENIRHHAG
                     MEYWTQGEDGKPLFIGRFHAAIAASTRGSTVCGLHITYLEAQRGGGFAKARIKHPQTG
                     EALPAKKMRSRKQGAMTGGAVYLFPVPENGRLMVSEGIETALAARELFNAHDWGLCAA
                     LSANGMKRLELSDGIKELAVIADHDAPRPVGETAALSLAKRAQSGGIAVRYWISGTAG
                     YDALDELNDRKCSGKA"
     gene            complement(3331..3552)
                     /locus_tag="ACFMWZ_001061"
     CDS             complement(3331..3552)
                     /locus_tag="ACFMWZ_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935034.1"
                     /translation="MRQDNRPRKTPYAGAAVFLIFGFAFAGMTHHAPENHTADIPPPV
                     ETQPQTPAIWETDPMAGVVLEPVSEEAEK"
     gene            complement(3707..3973)
                     /locus_tag="ACFMWZ_001062"
     CDS             complement(3707..3973)
                     /locus_tag="ACFMWZ_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935035.1"
                     /translation="MQSNFSQAAPSNQTQNIQAVHTQAQSYCAIVHEPTAYHYDADAY
                     SCTARRLGIYPTRAAAVSACLSFIAAITGDDFKAWASYQVVPAV"
     gene            4377..4718
                     /locus_tag="ACFMWZ_001063"
     CDS             4377..4718
                     /locus_tag="ACFMWZ_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFC5935036.1"
                     /translation="MKAVFVELPPFERFRADYFSSEKYRAFQNELMANPEKGDVIQGT
                     GGLRKIRVADSKRGKGKRGGSRVIYYWAVSRAIFLLFTVYGKNVKDDLSPSEKKILSA
                     MLHNLKENNHV"
     gene            4711..5031
                     /locus_tag="ACFMWZ_001064"
     CDS             4711..5031
                     /locus_tag="ACFMWZ_001064"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC5935037.1"
                     /translation="MSERNLFEELKEGLEAAEQYLQGKITLRAIEAEIPENGAAAISP
                     EEIRAIRESLNFSQAVFARKLRCSVRTYQGWEQGKTRPNPQAALLLRMVQKSPKTFKT
                     IAAL"
     gene            complement(5026..5280)
                     /locus_tag="ACFMWZ_001065"
     CDS             complement(5026..5280)
                     /locus_tag="ACFMWZ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC5935038.1"
                     /translation="MKHDCINDKPKTPNQRQRILARLRQGSVTSWELTQMGILGYNTR
                     IMELRQQGHNIVTTIEEIQNQFGETVKRGRFSLVSEGRQS"
     gene            complement(5342..5557)
                     /locus_tag="ACFMWZ_001066"
     CDS             complement(5342..5557)
                     /locus_tag="ACFMWZ_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC5935039.1"
                     /translation="MDQSVLRVNQVAERLGVSRSTVWNWGNTKSRHYRADFPRPFKVS
                     ANVVGWLSSEIDDYIGKLAAKREEQAG"
     gene            complement(5711..6403)
                     /locus_tag="ACFMWZ_001067"
     CDS             complement(5711..6403)
                     /locus_tag="ACFMWZ_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935040.1"
                     /translation="MSNNLTLADVPDFSDWRLAPLWTLEQAALLWAGINPAFSTFEDI
                     WKEHPRKQAWAKIALQAFLGGIVLKTLPVHELYLLDAGGWAYCDNQKTAIFRMNDIAP
                     KDTTIMQDVLIGWTSEERVFSLRQTIQAEEQARREAAKAEAEKQEVRALPYCVIPEFE
                     TPEFETACLVVRDFWNRQAVGVAPPKAIIIQEFIKNTFKNITGVEPKEAAIRRIDSLT
                     RPPLFKNQSKKQ"
     gene            complement(6407..7504)
                     /locus_tag="ACFMWZ_001068"
     CDS             complement(6407..7504)
                     /locus_tag="ACFMWZ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC5935041.1"
                     /translation="MRKNAMMYDPLYFPRSELAEIIAKSLYGGITHAFTLFAPRRMGK
                     TQFLLKDIKPTAEAAGFNVFYFSFMDSTAPTVDFQAALLRFAESIKTGGKVQNLIGQI
                     SKIEIMGSSIERRTERQQAELRLSDIIADIAGGSRPSLLLLDEIQELARQPDTGGLIR
                     SLRTGLDIHQQQVKTIFTGSSTNGLRAMFNDIKAPFFHFAHALDFPTLGREFTDFLAG
                     VYQERTGRGLDGGELYRAFERLNKTPLYMRAMLQDMIINPDLSIGQAESIRLEEMHKH
                     ADTAAQWRHLSLIEQLILSELANGQTAIYSAQTRQALARVIGVDDIGTSTVQNNIRKL
                     ERKDLITRDGANRLQINSPILKTWILENVER"
     gene            complement(7734..8150)
                     /locus_tag="ACFMWZ_001069"
     CDS             complement(7734..8150)
                     /locus_tag="ACFMWZ_001069"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC5935042.1"
                     /translation="MYLLDTNVISETRRANRADGNVRAWLNTIDPAEMYTSAIVMMEL
                     ERGVLGMERKDGQQGGILRRWLEEVVKPTFKDRILPIDETTAEICARLHIPDKSPEND
                     AWIAATAKQHGLTLATRNIKDFDHSGVKLINPFETV"
     gene            complement(8153..8428)
                     /locus_tag="ACFMWZ_001070"
     CDS             complement(8153..8428)
                     /locus_tag="ACFMWZ_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFC5935043.1"
                     /translation="MLNTVSSREFNQYPSQTQKKAAKSPVFITTRGKPSHVLISYAEY
                     RKLTGKPQSALEALTANAELAAKLESIDFEIPPRSTAQRPPVDFGED"
     gene            complement(8944..10083)
                     /locus_tag="ACFMWZ_001071"
     CDS             complement(8944..10083)
                     /locus_tag="ACFMWZ_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC5935044.1"
                     /translation="MYLYVNTSGGKLWRFDFSHNGKRKTLSIGKYPTVSLVEARQTAE
                     NARRLLAAGQDPGEAKQQEKRERQAAVSNTFRSIAAEWHKHRETGLTGNHAARVWDSL
                     EKDVFPMLGGLPVSEIKVSQVKEVIEAVAARGALETAGRILQRIKAVFTYAIRTERAE
                     NNPAVPLVGLIKAPKQKHQPALPQSELTEFYRRLMLEQAERQTKIAMQLIMLTFVRNG
                     ELRAAEWVEFDLNGAEWSIPAAKMKMKAPHIVPLADWTLELLAELKEITGHSRYLFPS
                     VKDHDKHMSENTLSYLMGRMGYKGIATPHGFRSLATDVLNENGFDSDVIERQLAHVEQ
                     NKVRAAYHRTEYIPQRKELMQWYSDFLRERYRQAEKELAGDTAKP"
BASE COUNT         2093 a         2651 c         2517 g         2927 t
ORIGIN      
        1 atatttagtc ttatctattt cattaaacag caatagacaa aaaaataacc gctctaaaag
       61 cggcagggtc tctctcgaaa tgaaagcata tcatgctgat ttaaatatat tttattttta
      121 gccaaatcaa taataccgta aaaaataccg taaatttgtc aaagtcaaaa tttttcaggg
      181 acggtttgtg gcatttctgc caagctgtga aatatgggca cattgtaaca tcggcaatca
      241 tgcgccgccc cgtataaaaa aacagtatat atataaaaat cacttgttac cgttgttact
      301 cttgttaccg cttcgcaaca ttctgttttt cggcttttcc tgtgccgtga tagccttttt
      361 ctttcgtttt cctgctctcg atagcgtaaa aaagccgcct ttcggcggct ctctgtatca
      421 atcaagttct agcggctcaa tgattaaata atggcgtatc ggcttcttgt tcactttgat
      481 ttggtgctgg tagcctgttt tggacggctt caagcgtcct tttcccgata gtaccttaca
      541 cacggtatga atcgggaacg attgggcgat ttcttctcta aacaccagcg gcaggatata
      601 aaactcgtca ttgccaccag cctgcacttt aaagcctgcg tgccctttgc ctttgccgcc
      661 tgtcatggtg tatggcgcgt ctctttcgct ccacgacagg aagcgcggga aatcggcata
      721 ttgtgcgata aagtcgtcca actggctgca tatcgcttct gcgtcgcggt ctcctgtgcc
      781 gttctcttcc agccattcgt gaaagcactg cataaccgcc gccccgcctg cctttaatcc
      841 tgtgatgggc gcggcatatt ctaaaacggt aaaacggcgg actatcctcc gtgcctgccc
      901 ttccataggc ggcaaggctt caaggtaccg ggcgcgtgtt gcttctatgg tttcggcggc
      961 ggcttctttg ccgtctgaaa ggatttggcg gataagggcg cgccctgccg tgccgtattt
     1021 cttggcggct gccctgttga tatgctccga cagtttcgcg ccgtctgtga agccgtgcag
     1081 cgtgtcgtat atgccgtgtt tggcttctgc ctgtatatcg ggcaatcggg cgcggttacc
     1141 tgccctccat tgggcgcggt ctccgattag gctttcgggg ttggtttcgc ccgttgagag
     1201 tatcaaattg cgccatgtct tttgtttgcg gttgccaccg tctttcgcgc cctgtgtttt
     1261 cgatatgccg ttaatcacgc tgtaaaccat ttgggggatt cttcgcgggt ctgcggtctg
     1321 tccgatttcg tccagtgcta aaaagccgtc gttacgcgct aaggcggcat tctgcaaacc
     1381 taatgcggta gtatcccaat tgctttttgt ggcttcatag ctgccccata cgcttaatgc
     1441 aaggttagcg gctgtggttt tgcctttgga cgaactgcct gccaagtgta cgccgccgcc
     1501 ctcttcgtgc aagagtgcca agaacggtgc ggcaaggctc aaacccaatg caaggcatag
     1561 gcggctgttg ccctctgcgt atcgggcggc ttcctgccgc cattcttcaa ggctgcccga
     1621 ttccgtatag ccctcccttt ggctggtgtc tccgttgtat aggatgtttg cgccctctgc
     1681 gttgatggtt tcgccattgg gcaggatata ggcgttgccg tgccagcctg cgcggtcggt
     1741 aatggtgtaa atatcgccgc tgccctgtgt ctgcaaatag tcggaaagcc gctctcgctt
     1801 aaccctgccc gataagacgg ccaagccgta agactgcaaa cgctgcccgc cttgaactgt
     1861 gccgatttcc gattgtggga tggcggcggt cttggttcgt cgtgtgttag cgtcctgcca
     1921 gcgtatcacg cggtaatatg cgccgtcgtt gtccgtgccc ctgcctatga tgtcgatggg
     1981 ggacgacagt tttaccggcg cggcttcctg cgtgttgccc tccctgtcgg tctttacgcc
     2041 gatataccat acgcccgcgc cgtccacgtc atagcgcggt tgtgcaaacg gcgcggcaaa
     2101 ttctgccata tcgcccaacg gggcggcttc ttctgtgaaa ttcggcttta cttctttaca
     2161 catagtgtta aaatctcctg tatgggtttg gttgcctttc gcggttttcc gcgtttggtt
     2221 tccgtttgat aaataatcca ttgccgccta caaatgccgc ctgtttttca ggcggcattt
     2281 tttacgcctt gccgctgcat ttgcggtcgt tcaattcgtc cagcgcgtca tagcccgccg
     2341 tgccgcttat ccagtatcgg acggctatcc cgccgctttg ggcgcgtttt gccaggctta
     2401 atgccgccgt ttcccctacg gggcgcggtg cgtcgtggtc tgcaataacg gcaagctctt
     2461 taatgccgtc tgaaagctcc agccgcttca taccgtttgc gcttagggcg gcgcataagc
     2521 cccaatcatg ggcgttgaac agttcacgcg cggctaaggc ggtctctatg ccctccgata
     2581 ccatcagccg cccgttttca gggacgggga acaggtaaac cgccccgccc gtcattgcgc
     2641 cctgtttgcg gcttctcatt ttcttggcgg gcaatgcttc gcccgtttgg gggtgcttaa
     2701 tccgcgcttt ggcaaagccg ccgccccgtt gtgcttccag ataggtaatg tgcaaaccgc
     2761 aaacagtgct gccgcgtgtg gaagccgcta ttgccgcgtg gaaacgcccg atgaacagcg
     2821 gtttgccgtc ctccccctgc gtccaatatt ccatgcctgc atgatggcgg atgttttcgg
     2881 gaagccgtgc tacggtcaaa ccgcgccccg cccaataccg gacaacgggg caatcgggcg
     2941 ttaatagctc tgcttcgtcc cacaatgcgg caagtttgcc gatatggtct ttttcggggc
     3001 gtggttgagg ctgtgggcgc gttggcggta tcggtaaggg gtttgtctcg tccatgccga
     3061 aaatgcccga aacctgcttt aatgccgtct gaaaatcgca acctagaaag tgcataacca
     3121 agccgaaacc gtcgcccgct ccgttctccc aatgggtgca aatgaatgtg cctttgccgt
     3181 ccttgtcgtc gtatctgaag cggtcttcgc ctccacacgc ggggcagggc tggtgtttgt
     3241 tgcgtaaaaa cttaggggct atgccgatgg cggcgtgtat ttcctgccag cggtaacgcg
     3301 cggcggcttt gatgtcttgg taagtcggtt tcatttttcc gcctcctccg atacaggctc
     3361 taacacaaca ccagccatcg ggtcggtttc ccatattgcg ggcgtttgcg gctgcgtctc
     3421 aacagggggc ggaatatccg ccgtgtggtt ttcgggggcg tggtgcgtca tgcctgcaaa
     3481 ggcaaaaccg aaaatcagga aaaccgccgc ccctgcatat ggcgttttgc gcggtctgtt
     3541 gtcttgtctc atggcttttc tcctgtgcca atgtttggaa taaacctaaa cccgaaacat
     3601 caaatataga taaatcaatg tgttatctaa aacataggtt tgagtctgcc cctaccgcct
     3661 aaaaataggc ggcatggcgg tttaggtttt gatgggtttt aaagggtcag acagcgggga
     3721 ctacttgata gcttgcccat gctttgaagt cgtcgcccgt aatggcggcg ataaaggaca
     3781 gacaggcgga aacggcggcg gcacgtgtgg ggtaaatccc caggcggcgg gcagtgcatg
     3841 aatatgcgtc agcgtcgtaa tggtaggcgg taggctcgtg aacgatggcg caatagcttt
     3901 gcgcttgtgt gtgaacggct tgaatgtttt gagtttggtt tgatggtgct gcttgagaaa
     3961 aattgctttg catttggaat gctcctatac gtttgagatt tgagaaatgc ccgaaaaggg
     4021 ggcggtgctc tctcctgcgt atagtcaggc gtgggcgttg ccgctaccac acaccgccat
     4081 aacttggata tgccgtctga agccttgaac agactgcaaa cagcataggg ggaaatggcg
     4141 ataaaaaatc agcgtttacc ctgctgattt tgggctatac gattttagag agaaaccgca
     4201 atctaatttc tcaatcccga tttgtaaata cttttcagac ggcattttta caagccgctg
     4261 attccattgc ctattaaata gcgttatacc gcgttgccgt acattggaag ccgtgcggcg
     4321 ttgcctttct atacgctttg cgcgtatgat ttggttttca ttcaaagggg cttttaatga
     4381 aagccgtatt tgtcgaactg ccgccgtttg aacgtttccg cgccgactac ttcagcagtg
     4441 agaaataccg cgcctttcaa aatgaattga tggcaaatcc cgaaaaaggc gatgtaatac
     4501 aagggacggg cggtctgcgt aaaatccgcg ttgccgattc caagcgcggc aagggcaaac
     4561 ggggcggatc tagggttatc tactattggg cggtcagccg tgccattttc cttttgttca
     4621 ctgtttacgg caaaaacgtt aaagatgatt tgtctccaag tgaaaaaaag atactttccg
     4681 ccatgcttca caacctcaag gaaaataacc atgtctgaac gcaatttgtt tgaagaactg
     4741 aaagagggct tggaagccgc cgaacaatat ttgcaaggga aaatcacatt gcgggcaata
     4801 gaagcggaaa tacccgaaaa cggggcggct gccatttctc ctgaagaaat acgcgccatt
     4861 cgcgaaagcc tgaatttctc ccaagcggtc tttgcccgca aactccgttg ctccgtccgt
     4921 acatatcagg gctgggaaca aggcaaaacg cgcccgaacc cccaagccgc cttattgctg
     4981 cgtatggtgc aaaaatcgcc taaaacgttt aagacgattg ccgcgctatg attgcctacc
     5041 ctccgacacc agcgaaaacc gcccgcgctt cacggtttcg ccaaattggt tttggatttc
     5101 ttctattgtg gtaacgatgt tatgcccctg ttggcgaagc tccataatgc gcgtgttgta
     5161 gccaagtatc cccatttggg taagctccca tgaggtaacg ctgccctgcc tcaatcgggc
     5221 taaaatgcgc tgtctttggt ttggtgtttt gggcttgtcg ttgatacaat catgcttcat
     5281 cattgattcc cctttccgcc gcctgaatgt ccgttttggc ggctttgttt tgcccggtaa
     5341 attaacccgc ctgctcttcg cgtttggctg ccagtttgcc gatatagtcg tcaatctcac
     5401 tggaaagcca gccaacgacg tttgcggata ctttgaacgg gcgcggaaaa tcggcgcggt
     5461 aatggcggct tttagtattg ccccaattcc acacggtcga acggctcacg cctaaacgct
     5521 cggctacctg attaacacgc aatactgatt ggtccatgct ttcgctccta tttggttgtg
     5581 ttggtacggc gtgaattgtc agatagccgt atagggttta ggcaataaaa aaacggttaa
     5641 accctccttt caagaaaggg gtttaaccgt cctgtaatgt atgattgata agggcttggg
     5701 ggttggctgc ttattgtttt ttagattggt ttttgaataa cggcgggcgc gtcaaactgt
     5761 caatgcgcct gattgccgcc tccttgggtt ctacccctgt tatattctta aaggtgttct
     5821 taataaactc ttgaataata attgccttag ggggtgcaac ccctaccgcc tgcctgttcc
     5881 aaaaatccct caccactaaa caggcggttt caaattcagg ggtttcaaat tcgggaataa
     5941 cgcaataagg caatgccctc acttcctgtt tttctgcctc tgctttagcc gcttcccgcc
     6001 ttgcctgttc ttctgcctga atggtttgcc tcaagctaaa cacacgttct tccgatgtcc
     6061 agccaattag cacatcttgc ataatggtcg tgtcctttgg tgcgatgtca ttcattctaa
     6121 aaatagcagt tttttggttg tcgcaataag cccaaccgcc tgcatcaagc aaatacaact
     6181 catgcaccgg cagtgttttt aaaacaatac cgcccaaaaa agcctgtaat gcaatttttg
     6241 cccacgcctg tttgcgcggg tgttccttcc atatatcctc aaaagtggaa aacgccgggt
     6301 taattcccgc ccacaataaa gcggcttgtt ccagcgtcca caacggggct aaacgccaat
     6361 cagaaaaatc aggaacatca gcaagggtta aattgttact cattccctac cgctccacat
     6421 tctctaaaat ccaagtcttt aaaatcgggc tgtttatctg caacctgttc gcgccgtctc
     6481 tggttatcag gtctttacgc tccaatttgc ggatattgtt ttgcaccgtg cttgtgccta
     6541 tatcgtccac accgattaca cgggctaggg cttggcgcgt ttgcgcggaa tatatcgcgg
     6601 tctgcccgtt tgccaactcc gataaaatca actgttctat caggctcaaa tgccgccatt
     6661 gcgcggctgt gtctgcgtgt ttgtgcattt cctccagcct tatgctttca gcctgcccta
     6721 tgcttaaatc agggttgatt atcatgtcct gcaacattgc ccgcatataa aggggtgttt
     6781 tgttcagccg ctcaaatgcg cggtacagtt cgccgccgtc taaccccctg cccgtcctct
     6841 cctgatacac accggcaaga aaatcggtaa attcccgtcc aagcgtggga aagtctaagg
     6901 cgtgggcgaa atggaaaaac ggggctttga tgtcgttgaa catggcgcgt aaaccgtttg
     6961 tgctgctgcc cgtgaatatg gtttttacct gctgctggtg tatgtccaag cctgtacgca
     7021 agctgcgtat caagccgccc gtatcaggtt gccgtgccag ttcctgtatt tcgtccaaca
     7081 gcaacaggct ggggcggctg ccccctgcaa tgtcggcgat gatgtcggac agccgcaatt
     7141 ccgcctgctg ccgctccgtc cgtctctcta tgctgctgcc catgatttct attttgctga
     7201 tttgcccaat caggttttgc acctttccgc ccgtcttgat actttcggca aagcgcaata
     7261 atgccgcctg aaagtcaaca gtgggcgcgg tgctgtccat gaatgagaaa taaaagacat
     7321 tgaagcccgc cgcctctgcc gtgggcttga tgtctttcaa aagaaactgt gttttgccca
     7381 ttcttcgcgg cgcaaacagc gtaaaggcgt gtgttatgcc tccatacagg cttttggcga
     7441 tgatttccgc caactctgaa cgggggaaat ataaggggtc atacatcatt gcattctttc
     7501 gcattgttac tcattagccc gtattataca ccgtaaagct aattattaat acggtcaata
     7561 attttgtata acgcgcggcg caccgtcagc gcgttttagc tggtgtaggt gtgaaaaatc
     7621 cccgtacctg tcggttatac ggggatgttt tcggcggctt tcgcgcctgt ttgcgccgcg
     7681 cctttcatat tggttatgcc gtccttgtta tccattcgtt gaggctgata tttttagacg
     7741 gtctcaaacg ggtttatcaa tttcacgccg ctatgatcaa agtctttgat atttcgcgtt
     7801 gccaaagtta aaccgtgctg cttggctgtt gccgctatcc aggcgtcgtt ttcggggctt
     7861 ttgtcgggta tgtgcaaacg ggcgcatatt tcggcggtgg tttcgtctat gggcaatatg
     7921 cggtctttga atgtgggctt tacaacttct tccagccagc ggcgcaatat tccgccctgc
     7981 tgtccgtctt tgcgctccat acccaaaacg ccccgttcaa gttccatcat gacaattgcg
     8041 ctggtgtaca tttcggccgg gtcaatggtg tttagccatg ctctaacgtt gccgtctgcc
     8101 cgatttgccc ggcgtgtttc gctgataacg ttggtatcca acagatacat tttcaatcct
     8161 ccccaaaatc aacgggcggt ctttgcgcgg tgcttcgcgg cggtatttca aaatcgatgc
     8221 tttccagttt ggcggcaagc tcggcgtttg ccgtcagtgc ctccagtgcg ctttgcggct
     8281 tgcctgtcag tttgcggtat tccgcataac tgattaatac atggctcggc ttgccgcgtg
     8341 tggtaataaa tacgggggac tttgcggctt tcttttgtgt ttggctgggg tattggttaa
     8401 attccctgct gctgacagtg tttaacatta cgccctcctt gtgtcttgtt tgtagttatg
     8461 tagcctcttt ttacgcgatt gtgcctgtcg tgtcaatgtg cattgccctt gtgcctgtgt
     8521 tgccgtctga aacaccagcg gccaacctga ttgtttttta atcaatcgct tccaatggct
     8581 caaatttgcg tttttagagc gttttatagt ctgcctatgc cgtgatatat ccaagcggtt
     8641 tagctgccat tcctgctcaa atttggcgcg tttccatgcg ttttagcttt tcattccgct
     8701 acgctcccac tgccaagaca ggggaaaagc cgaaaatgcg gggcagttgt taccacttgt
     8761 tacgatttgc taccgcactt gttaccgatt ttttgtcttt ttcttatttt atttcatata
     8821 tttatttatc taactttttc ccagtaacaa cgtaacaact aggggtatgg tattcctata
     8881 tagggatttt cagaaagcca aaaaaaaccg tcctgcatct tggacggtct cccccctcat
     8941 tccctacggc ttggcggtat ctcctgcaag ctctttttcc gcttggcggt atctctcccg
     9001 caaaaaatca ctataccatt gcataagctc tttacgctgt gggatatatt ccgttctgtg
     9061 gtatgccgcc ctgactttgt tttgttccac atgggcaagc tgtcgctcta tcacatcact
     9121 gtcaaagccg ttctcattca aaacatctgt ggctaggctt ctaaagccgt gcggtgttgc
     9181 tatgcccttg tagcccattc tgcccattaa gtaagacagg gtgttttcac tcatgtgctt
     9241 gtcatggtct tttaccgatg ggaataaata gcggctatgc cctgttattt cctttagttc
     9301 tgccaaaagc tcaagcgtcc aatcagcaag aggaacgata tgcggggctt tcatcttcat
     9361 cttggcggca ggtatgctcc attctgcccc gtttaaatca aattccaccc attccgccgc
     9421 cctaagctct ccattcctga caaaagtcag catgattaac tgcatggcta tttttgtttg
     9481 ccgttcggct tgttccaaca tcaaacggcg gtaaaactcg gtcagctcgg attgcggtaa
     9541 tgcgggctgg tgtttctgct ttggtgcttt aattaaacct accaaaggga ctgcgggatt
     9601 attctctgcc cgctccgtcc gtatggcata agtgaaaaca gccttgatac gctgcaatat
     9661 ccgccctgcc gtttcaagtg ctcctcttgc cgctaccgct tcaatcactt cttttacttg
     9721 gctgactttg atttcactga caggcaaacc gccaagcatg gggaaaacgt ccttttctaa
     9781 actatcccat acacgggcgg catgattgcc cgtcaatcct gtttcacggt gtttgtgcca
     9841 ttcggcggca atgctccgaa atgtgttact gacagcggct tgccgttcac gcttttcctg
     9901 ctgcttggct tcgccggggt cttgccccgc tgcaagcaag cggcgggcat tttcagcggt
     9961 ttggcgggct tccaccagtg atacggtcgg atatttgccg atagaaagtg ttttgcgttt
    10021 gccgttatgc gaaaagtcaa accgccataa cttcccgccg ctcgtattga catacagata
    10081 caaacctttg ccgtcattca gcttatacgg ttttttcggc gggcttggcg tttttgattt
    10141 gtcgatcgtt cagcttcatt tttactttga gacctttgca aaattcct
//
LOCUS       DAXHKX010000054        10168 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHKX010000054 DAXHKX010000000
VERSION     DAXHKX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.054"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(122..523)
                     /locus_tag="ACFMWZ_001072"
     CDS             complement(122..523)
                     /locus_tag="ACFMWZ_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935045.1"
                     /translation="MAIYDLNEIVTSAALGMYKGKWQNMDFEQVLQDIVQETDFTYSW
                     EYGENWAIFCLNEKSVGMLCKQIPFAIIENEKLSQKIYQEYPWILTQQVYHFDTDKIL
                     LNKYVCKLTIGIMSAISQEPISLQDLYVDTV"
     gene            complement(628..831)
                     /locus_tag="ACFMWZ_001073"
     CDS             complement(628..831)
                     /locus_tag="ACFMWZ_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC5935046.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     EDKARYDAQRRKLKEAGWSDDAV"
     gene            complement(809..1042)
                     /locus_tag="ACFMWZ_001074"
     CDS             complement(809..1042)
                     /locus_tag="ACFMWZ_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC5935047.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            1236..1364
                     /locus_tag="ACFMWZ_001075"
     CDS             1236..1364
                     /locus_tag="ACFMWZ_001075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935048.1"
                     /translation="MTLMHILKHELPDTLVIGISHQNEIKNLFERKINLTACHPSE"
     gene            1582..5016
                     /gene="dnaE"
                     /locus_tag="ACFMWZ_001076"
     CDS             1582..5016
                     /gene="dnaE"
                     /locus_tag="ACFMWZ_001076"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC5935049.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAAKMPEYQKRLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     NFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            5295..8543
                     /gene="cas9"
                     /locus_tag="ACFMWZ_001077"
                     /gene_synonym="csn1"
     CDS             5295..8543
                     /gene="cas9"
                     /locus_tag="ACFMWZ_001077"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HFC5935050.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAAARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            8610..9524
                     /gene="cas1"
                     /locus_tag="ACFMWZ_001078"
     CDS             8610..9524
                     /gene="cas1"
                     /locus_tag="ACFMWZ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC5935051.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAKHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            9517..9843
                     /gene="cas2"
                     /locus_tag="ACFMWZ_001079"
     CDS             9517..9843
                     /gene="cas2"
                     /locus_tag="ACFMWZ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFC5935052.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
BASE COUNT         2839 a         2720 c         2379 g         2230 t
ORIGIN      
        1 agttatcctg ctgtatccat taaatcatat gcttcaatta attgttgttc tagctcttgt
       61 accaattttg gattgcgaat tcctaactca atttcaaaca gcgagttctc actatgtaca
      121 tctacacagt atctacataa agatcttgta aagatatagg ttcttggctg atggcactca
      181 ttattccaat agttaatttg catacatatt tgtttaataa aattttatct gtatcaaaat
      241 gataaacttg ttgagttagt atccaaggat attcttggta tattttctgt gataattttt
      301 cattctctat aatcgcgaaa ggtatctgtt tacataacat accaacagac ttttcgttta
      361 gacagaaaat agcccaattt tccccatatt cccaagagta tgtaaaatct gtttcctgaa
      421 caatatcttg taaaacttgc tcaaaatcca tgttttgcca tttaccttta tacataccta
      481 atgcggcact ggttactatt tcgtttaagt cataaatagc catttatgtt ccttgctaat
      541 ttctaggtga gacatgtata ggactgagac cagtatgccc attaggttcc cctctataca
      601 cattaatagt agatgttggt tccgttttca gacggcatcg tccgaccaac ccgcttcttt
      661 caatttccgc cgttgcgcgt cgtagcgggc cttatcctcc caataaatcg tctgaataca
      721 cgcctcgccg cagtcgctgc cgcagcattc ccacgactcg ggtcggacgg gttcgtctaa
      781 aagcggctcg cccaaaaggg cttcggcttt atgcttcagg gtcatatcca tcggcgattt
      841 ccaagcgcgc accgtcaaac tcgatgactt cgccgccgcg tattttggcg gttttacggg
      901 tttcggtttc gccgttgcgc gacaccaacc cttcggcaat caacgccttc gcctgtccgc
      961 cgctttcggc aagtccggcc aatttcaaga ggtcgcacag ggcgatgtat tcgttgtctt
     1021 cgagatagac ggtggcttcc ataatgttcc cttgcagaaa gaggccgtta ttgtagcacc
     1081 tgccgccgcc gtacccaaaa ttaccgcaaa acaggcagca catcccgccc cgcctgttcc
     1141 gtaaaaatgc cgtctgaaac cccatatgcc gtcatccgat caaaaaaatc ctgcccaaca
     1201 gcaggctgca aatcctgttc cccgacgaat ccgcattgac gctgatgcac atcctcaaac
     1261 acgaactgcc cgatacactg gtcatcggca tcagccatca aaacgaaatc aaaaacctgt
     1321 ttgaacgtaa aattaattta actgcttgcc atccgtccga ataaggtata tagttcctta
     1381 taactagttt gatagtcctt tatatctatc aatactcctt gggaagcctc caccacacgg
     1441 taggaggcat ttttttgccg tcgtaaaagc tcaaaaacat ttgcaggtca tgccgtctga
     1501 atccgaaaca gcattaccta caccgccatc taaagacaac cctgctacaa tacgcctttt
     1561 attgttcacg ccgattttgc catgaccgag ccgacctaca tccccctgcg cctgcatacc
     1621 gagttctcaa ttaccgacgg tatggtgcgg attaaaaaac tgattgccaa agcgcaggaa
     1681 tacggtttgc ctgctttggg catcagcgat ttgatgaacg aattcggttt ggtgaaattc
     1741 tataaagcct gccgcggcgc agggattaag cccatcggcg cggcagatgt atggataggc
     1801 aatccgaatg cgcccgacaa gccgttccgc gccatgctga ttatccgcaa cgatgcgggc
     1861 tatctgcgcc tgagcgagct tttgacggcg gcttatgtcg gcaaagaccg caatgtccat
     1921 catgcggaac tcaatcccga atggctggaa aacggcgaca acagcggctt gatttgtttg
     1981 agcggcgcgc attacggcga agtgggcgtg aatctgttaa acggcaatga agacgcggcg
     2041 cgtgcggcgg cgttgaaata tgcagcgtgg ttccccgatg cgttttattt agagctgcaa
     2101 cgcctgcccg aacgccccga atgggaggct tgcgtttccg gcagcgtgaa gctggcggag
     2161 gaattgggtt tgccggtggt ggcgacgcat ccgacacagt ttatgagccg cgacgatttc
     2221 aacgcgcacg aggcgcgggt gtgcatcgcc ggcggctggg tgctgacgga caagaaacgt
     2281 ccgcgcgatt tcacgccggg tcagtttttc attccgccgg aaacgatggc cgaacgtttc
     2341 gccgatttgc ctgaagcctt ggaaaacacg gtagaaattg ccaaacgctg caacctgcac
     2401 atcacattgg gcaaaaactt cctgcccctt ttccccacgc ccgacggttt atcactcgat
     2461 gactatctca tcaaactctc caacgagggt ttgcaggaac gtatggttca gctttatccc
     2521 gacgaggcgg agcgggcggc gaaaatgccg gaatatcaaa aacggctgga ttttgagctg
     2581 aacatcatta tccagatgaa attccccggc tatttcctta tcgtacaaga ctttatcaac
     2641 tgggcgaaaa cgcatggctg tccggtcggg ccgggacgcg gttcgggcgc gggttcgctg
     2701 gtggcgtatt cgctgaaaat taccgacctc gacccgctga aatacgcgct gctgtttgaa
     2761 cgtttcttaa accccgaacg cgtctccatg cccgacttcg acgttgactt ctgtcaggca
     2821 aaccgcggcc gcgtgattga gtatgtgcgc gaaaaatacg gcgcggaggc ggtcagccag
     2881 attgttacct tcggcacgat gtcgtccaaa gcagtcatcc gcgacgtggg gcgcgtgtta
     2941 gagctgccgt ttatgctgtg cgacaaactg tccaagctga ttccgttgga agccaacaaa
     3001 cctctgggtt tggacgacgc gatgaaggcg gagccgcaga ttcaggaatt aatcgaagcg
     3061 gaagaagcgg acgaactgat tacgctggcg aaaaagctgg aagacctgac gcgcggtttg
     3121 ggtatgcacg cgggcggcgt gttgattgcg ccgggcaaga tttccgatta cagccccgtg
     3181 tatcaagcgg acgaatccgc ctcgcccgta tccatgtacg acaagggcga cgtggaagac
     3241 gtgggtttgg tgaaattcga ctttttgggt ctgcgcaact taaccattat cgaaatggcg
     3301 cagaacaaca tcaaaaacac caccggcgat atcgtcgatg ttggcaagat tccgcttgac
     3361 gaccaggccg cctaccaaat cttccgcgat gcgaacacca ccgccgtctt ccagttcgag
     3421 tcgaccggca tgaaaaaaat gctgaaaacg gcgcacacga ccaagtttga agaactcatc
     3481 gccttcgtat cgctctaccg ccccggcccg atggacaaca tccccgactt cgtcgcgcgt
     3541 atgaagggac aggaattcca atacatccac ccgctgctcg aaggcatcct tgcgccgact
     3601 tacgggatta tggtgtatca ggaacaagtg atgcaggcgg cgcagattat cggcggctac
     3661 tcactcggtg gtgcagactt gttgcgccgc gccatgggta agaaaaaacc cgaagaaatg
     3721 gtgaaacacc gcgaaatctt cgctgaaggc gcggccaaac aagggatttc gcgcgaaaaa
     3781 tccgacgaaa tcttcaacta catggaaaaa ttcgccggct acggtttcaa caaatcccac
     3841 gccgccgcct acgccctgat ttcctatcag accgcatggc tcaaagccca ctatcccgcc
     3901 gaatttatgg cggcaaccat gtcgtctgaa ttggacaata ccgaccagct caaacatttc
     3961 tacgacgact gccgcgccaa cggcatcgag ttcctgccgc ccgacatcaa cgaatccgac
     4021 taccgcttca cgccgtatcc gaacatgaaa atccgctacg cactcggcgc gattaaaggc
     4081 acgggcgaag ccgccgtcga atccatcatc gccgcgcggc aaagcggcgg caagtttacc
     4141 ggcctcttga acttctgcga gcgcgtcggc aaagaacaca tgaaccgccg caccctcgaa
     4201 gctctgatac gcggcggcgc attcgacagc atcgaaccca accgcgccat gctcttggcg
     4261 aacatcgacc tcgctatgga caacgccgac caaaaagccg ccaacgccaa tcagggcggg
     4321 cttttcgaca tgatggaaga cgccatcgaa ccggtgcggc tcatcgacgc gccgatgtgg
     4381 agcgaatcgg aaaaactcgc cgaagaaaaa accgtcatcg gcttttacct gtccggccac
     4441 ccgttcggcc cgtatgccca agaagtccgc caaatcgccc cgcagaaact gtcgaaactc
     4501 aaaccgcaag acagcgtgcg cctcgccgga ttcgttaccg ccgtacgcac catgatgggc
     4561 aagcgcggca aaatcgcctt cgtcagcctt gaagatttga gcggacaggt tgaaatcatg
     4621 gtcggcggtc agacgttgga aaactgcgcc gactgcctca aagccgacca agtgctgatt
     4681 atcgaatcca aagtcagccg cgacgactac ggcggcggcg acggactgcg tattctggca
     4741 aaccaagtca tgaccctgca aacggcacgc gaacgctacg cccgcagcct cagcctcgcc
     4801 ctcgccccgc atcacgacat cggcggactg gtacaactgc tcgccgccca ccaactgccc
     4861 gacacgccgc gcatcccgct gcaactgtcg tatgccaacg aaaaagcgtc gggcaggctt
     4921 caagtgccgc cgaaatggac ggttacaccg agctccgcat tgttcggcga actggaaaca
     4981 ttgctcggca gccggtcggt gcgcgtcaac tggtaaccaa aaatagaaat gccgtctgaa
     5041 gcccaaaaac cggtttcaga cggcattttt taaccgaaat aaacgatgcc ccttaaagca
     5101 gaagctttaa ggggcagagc gttgcggcac atcttttcag acggccttat tgtagcaacg
     5161 gttctcattt cgcagtgcga caatatgttt gggattctag ccgttgtgag atgataagtc
     5221 aataatctat ttgtatataa ataagacaat cagatatatt atcattattt gcaacgttgt
     5281 ttacggagta ataaatggct gccttcaaac ctaatccaat caactacatc ctcggcctcg
     5341 atatcggcat cgcatccgtc ggctgggcga tggtagaaat tgacgaagat gaaaacccaa
     5401 tctgcctgat tgatttaggc gtgcgcgtat ttgagcgcgc agaagtaccg aaaacaggtg
     5461 actctctcgc agcagcaaga cgcttggcac gcagcgtccg ccgcctgacc cgccgtcgcg
     5521 cccatcgcct gcttcgggcc cgccgcctat tgaaacgcga aggcgtatta caagccgctg
     5581 attttgacga aaacggcttg atcaaatcct taccgaatac accatggcaa cttcgcgcag
     5641 ccgcattaga ccgcaaactg acgcctttag agtggtcggc agtcttgttg catttaatca
     5701 aacaccgcgg ttatttgtcg caaagaaaaa acgagggcga aactgccgat aaagagcttg
     5761 gcgctttgct caaaggcgta gccgacaatg cccatgcctt acagacaggc gatttccgca
     5821 caccggccga attggcttta aataaatttg agaaagaaag cggccatatc cgcaatcagc
     5881 gcggcgatta ttcgcatacg ttcagccgca aagatttgca ggcggagctg attttgctgt
     5941 ttgaaaaaca aaaagaattt ggcaatccgc atgtttcagg cggccttaaa gaaggtattg
     6001 aaaccctact gatgacgcaa cgccctgccc tgtccggcga tgccgttcaa aaaatgttgg
     6061 ggcattgcac cttcgaaccg gcagagccga aagccgctaa aaacacctac acagccgaac
     6121 gtttcatctg gctgaccaag ctgaacaacc tgcgtatttt agagcaaggc agcgagcggc
     6181 cattgaccga taccgaacgc gccacgctta tggacgagcc atacagaaaa tccaaactga
     6241 cttacgcaca agcccgtaag ctgctgggtt tagaagatac cgcctttttc aaaggcttgc
     6301 gctatggtaa agacaatgcc gaagcctcaa cattgatgga aatgaaggcc taccatgcca
     6361 tcagccgtgc actggaaaaa gaaggattga aagacaaaaa atccccatta aacctttctc
     6421 ccgaattaca agacgaaatc ggcacggcat tctccctgtt caaaaccgat gaagacatta
     6481 caggccgtct gaaagaccgt gttcagcccg aaatcttaga agcgctgttg aaacacatca
     6541 gcttcgataa gttcgtccaa atttccttga aagcattgcg ccgaattgtg cctctaatgg
     6601 aacaaggcaa acgttacgat gaagcctgcg ccgaaatcta cggagaccat tacggcaaga
     6661 agaatacgga agaaaagatt tatctgccgc cgattcccgc cgacgaaatc cgcaaccccg
     6721 tcgtcttgcg cgccttatct caagcacgta aggtcattaa cggcgtggta cgccgttacg
     6781 gctccccagc tcgtatccat attgaaactg caagggaagt aggtaaatcg tttaaagacc
     6841 gcaaagaaat tgagaaacgc caagaagaaa accgcaaaga cagggaaaaa gccgccgcca
     6901 aattccgaga gtatttcccc aattttgtcg gagaacccaa atccaaagat attctgaaac
     6961 tgcgcctgta cgagcaacaa cacggcaaat gcctgtattc gggcaaagaa atcaacttag
     7021 gccgtctgaa cgaaaaaggc tatgtcgaaa tcgaccatgc cctgccgttc tcgcgcacat
     7081 gggacgacag tttcaacaat aaagtactgg tattgggcag cgaaaaccaa aacaaaggca
     7141 atcaaacccc ttacgaatac ttcaacggca aagacaacag ccgcgaatgg caggaattta
     7201 aagcgcgtgt cgaaaccagc cgtttcccgc gcagtaaaaa acaacggatt ctgctgcaaa
     7261 aattcgatga agacggcttt aaagaacgca atctgaacga cacgcgctac gtcaaccgtt
     7321 tcctgtgcca atttgttgcc gaccgtatgc ggctgacagg taaaggcaag aaacgtgtct
     7381 ttgcatccaa cggacaaatt accaatctgt tgcgcggctt ttggggattg cgcaaagtgc
     7441 gtgcggaaaa cgaccgccat cacgccttgg acgccgtcgt cgttgcctgc tcgaccgttg
     7501 ccatgcagca gaaaattacc cgttttgtac gctataaaga gatgaacgcg tttgacggta
     7561 aaaccataga caaagaaaca ggagaagtgc tgcatcaaaa aacacacttc ccacaacctt
     7621 gggaattttt cgcacaagaa gtcatgattc gcgtcttcgg caaaccggac ggcaaacccg
     7681 aattcgaaga agccgatacc ccagaaaaac tgcgcacgtt gcttgccgaa aaattatcat
     7741 ctcgccccga agccgtacac gaatacgtta cgccactgtt tgtttcacgc gcgcccaatc
     7801 ggaagatgag cgggcaaggg catatggaga ccgtcaaatc cgccaaacga ctggacgaag
     7861 gcgtcagcgt gttgcgcgta ccgttaacac agttaaaact gaaagacttg gaaaaaatgg
     7921 tcaatcggga gcgcgaacct aagctatacg aagcactgaa agcacggctg gaagcacata
     7981 aagacgatcc tgccaaagcc tttgccgagc cgttttacaa atacgataaa gcaggcaacc
     8041 gcacccaaca ggtaaaagcc gtacgcgtag agcaagtaca gaaaaccggc gtatgggtgc
     8101 gcaaccataa cggtattgcc gacaacgcaa ccatggtgcg cgtagatgtg tttgagaaag
     8161 gcgacaagta ttatctggta ccgatttaca gttggcaggt agcgaaaggg attttgccgg
     8221 atagggctgt tgtacaagga aaagatgaag aagattggca acttattgat gatagtttca
     8281 actttaaatt ctcattacac cctaatgatt tagtcgaggt tataacaaaa aaagctagaa
     8341 tgtttggtta ctttgccagc tgccatcgag gcacaggtaa tatcaatata cgcattcatg
     8401 atcttgatca taaaattggc aaaaatggaa tactggaagg tatcggcgtc aaaaccgccc
     8461 tttcattcca aaaataccaa attgacgaac tgggcaaaga aatcagacca tgccgtctga
     8521 aaaaacgccc gcctgtccgt taaattaatc tatccctgtt tcaggcggcc tgaagcaggg
     8581 attttttata taaaaataag gggaaaaaca tgacttggcg cagcctgctt attcaaaacg
     8641 gcggcaaact atccttgcaa cgccggcaac tgctgattca acaaaacggc gagtcccaca
     8701 ccgtaccatt agaggacatc gccgttatca tcatcgaaaa tcgcgaaacc ctgattaccg
     8761 ccccgctcct atcagcctta gccaagcatg gtgcaaccct gctgacctgc gacgagcagt
     8821 tcctgccctg cggacaatgg ctgccttatg cccaatatca ccgccaactt aaaatcctca
     8881 aactgcaact gaacataagc gaacccttaa aaaagcaact ctggcaacat atcgtccgcc
     8941 aaaaaatcct gaaccaagca tttgtcgcag acgaaacagg caatgatctt gcagccaaac
     9001 ggctgcgcac tttggcatct gaagtccgtt caggcgatac aggaaaccgt gaagcccaag
     9061 ctgccgccct ctactttcaa gccctctttg gcgaaaaatt tacccgtaat gacaacaacg
     9121 ccgtcaatgc cgcattgaac tacacctacg ccgtactccg tgccgcagtt gcacgcgccc
     9181 tgaccttata cggttggtta cctgcattag gcttgtttca tagaagtgaa ctcaatccat
     9241 ttaatctcgc cgatgacttt atcgaaccct tacgcccatt ggccgacctg accgtgatac
     9301 atttgtacga acaaggccgt ctgaaaactg aactgacgct cggcatcaaa caacacctta
     9361 tcaaaatcct ctactatcaa accagcatcg aacggcaaca tttcagcacc ttggctgcca
     9421 tcgacaaaat gatttcgtca tttcaagccg gcgtaaccga taaaaacgcc aaacaactga
     9481 aactgcctga aatcctacca ttgaaagaat accaatatga gtgaggccaa atttatgagg
     9541 attatcgtct tcttcgacct accggttatc acggcggcaa agcgcaaagc cgccaatcaa
     9601 ttccgccagt ttttattaaa agacggatac caaatgctgc aactttccgt atacagccgt
     9661 atcgtcaaag gccgcgattc gttgcaaaaa caccacaaca gactgtgtgc aaaccttccg
     9721 caagaaggct caatccgctg tttggagata acagaaaagc aatatgccgc catgaaactg
     9781 ctgttgggcg agctgaaaac ccaagaaaaa aaggtcaatt cagaccaatt attgttattt
     9841 taagcccatt ttttcataac aaataaaacg ggaaaccctt atgaaataag gatttcccgt
     9901 cgaagtattg tagcactgcg aaatgagaaa gggagctaca acgacaaaac tagcaacctc
     9961 aatcgggaag aaattgtagc actgcgaaat gagaaaggga gctacaacca aactacacaa
    10021 tgcagcaagg cgcaaattat tgtagcactg cgaaatgaga aagggagcta caaccagtgc
    10081 tgcaaagcat ggaggctttc cgcaattgta gcactgcgaa atgagaaagg gagctacaac
    10141 ttttcatcta caattttgat atatcttg
//
LOCUS       DAXHKX010000055        10045 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHKX010000055 DAXHKX010000000
VERSION     DAXHKX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.055"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(118..1335)
                     /locus_tag="ACFMWZ_001080"
     CDS             complement(118..1335)
                     /locus_tag="ACFMWZ_001080"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC5935053.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1537..2265)
                     /gene="rph"
                     /locus_tag="ACFMWZ_001081"
     CDS             complement(1537..2265)
                     /gene="rph"
                     /locus_tag="ACFMWZ_001081"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC5935054.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2348..2812)
                     /locus_tag="ACFMWZ_001082"
     CDS             complement(2348..2812)
                     /locus_tag="ACFMWZ_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC5935055.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAIAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3029..3805
                     /locus_tag="ACFMWZ_001083"
     CDS             3029..3805
                     /locus_tag="ACFMWZ_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC5935056.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     IEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVFSTENIVPPQSGVAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            3809..3940
                     /locus_tag="ACFMWZ_001084"
     CDS             3809..3940
                     /locus_tag="ACFMWZ_001084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935057.1"
                     /translation="MPSETGFRRHLNKRGNQAVGSEKPTSARLKPYSQPAPFQNICI"
     gene            4050..4904
                     /locus_tag="ACFMWZ_001085"
     CDS             4050..4904
                     /locus_tag="ACFMWZ_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC5935058.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4945..6153
                     /gene="pncB"
                     /locus_tag="ACFMWZ_001086"
     CDS             4945..6153
                     /gene="pncB"
                     /locus_tag="ACFMWZ_001086"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC5935059.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEVELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6245..7963
                     /gene="argS"
                     /locus_tag="ACFMWZ_001087"
     CDS             6245..7963
                     /gene="argS"
                     /locus_tag="ACFMWZ_001087"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC5935060.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDADAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8529..9947
                     /locus_tag="ACFMWZ_001088"
     CDS             8529..9947
                     /locus_tag="ACFMWZ_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC5935061.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2302 a         2957 c         2542 g         2244 t
ORIGIN      
        1 taatccacta tattttttgt tccaaagtca aaatatgccg tccgaacatt cgggcggcag
       61 acaaaacggc actgcccgat aaaggcagtg ccgttgtccg tttcaaaccg tgaaacatca
      121 gcccaaatta aaggctttat gcaataccct ggttgccagt tccatgtatt tttcatcaat
      181 caatacggaa actttgattt cggaggtgga aatcatttgg atgttgatac cctcttcggc
      241 gagcgtgcgg aagattttgg cggctacgcc gacgtgcgaa cgcataccca aaccgactgc
      301 ggagactttg catacggtgt cgtcgccatc aatagaagcc gcgccgatac tgtcttggcg
      361 ttccgacagg atttccaaag tctgcttgta atcgccgcgc ggtacggtaa aggaaaaatc
      421 ggttgtgcct tcgctgccga cattttggat aatcatatcg acttcgatgt tggcatcggc
      481 aaccgcgcct aaaatctgat aggcaacgcc gggtttgtcg ggtacgccgc gcacgttgat
      541 gcgggcttgg tttttatcga atgcgatacc ggttacggca gctctttcca tgttgtcgtc
      601 ctcttcaaag gtaattaagg tgccattgcc gccgtcttgc aggctgctca atacgcgcag
      661 gcgcactttg tattttccgg cgaattctac tgaacggatt tgcaaaactt tcgaaccgag
      721 gcttgccagt tcgatcattt cttcaaatgt aaccgtatcc atgcggcgcg cttcgggtac
      781 gacgcggggg tcggttgtgt aaacgccgtc tacgtcggta tagatttggc actcgtcggc
      841 tttgagggct gcggcaagcg cgacggcgga agtgtcggaa ccgccgcgtc cgagcgtgga
      901 aatattgcct tcactgctga tgccttggaa gccggcaacg atgacgactt tgcctgcatc
      961 gaggtcggca cgcatttttt cgtcatcaat gctttcgatg cgggctttgg tgtgggcggt
     1021 atcggttttg agggcgacct gccagccggt gtagcttttg gcttccacgc cgatgtcttt
     1081 caatgccatc gccaaaaggc cgatggttac ttgttcgccg gtggacaaaa ccacgtccaa
     1141 ttcgcgcgga tcgggatgct cctgcatttc gtgcgccaac gcgaccaggc ggttggtttc
     1201 accgctcatg gcggatacga cgacgacaat gtcgtgtcct tcggcgcggg ctttggcgac
     1261 acgtttggcc acgtttttaa tgcgttcggg cgagcctacg gatgtgccgc cgtatttgtg
     1321 tacgattaac gccatgtttc gtgctttctt gtgtgggatt ggtcgggcag catggtttgc
     1381 tggaaaagcg gctattatta ctatttttta catgaagttc aagaacggac tgcgctttcc
     1441 cgcctgccgt ttgacagcgg tcagcgaaaa acctgttctt tcagattgtt gacaaaatgc
     1501 cgtctgaacg gttttcagac ggcatccgga cgacaatcag gcggcggaca acgcattttg
     1561 ctggtgttgc agcagttcgc ctatgccttt ttgcgccagt gcaaccagtt tgcccaattc
     1621 gtccaaactg aacggcgcgt cttccgccgt accttggatt tcgatgattt ttcccgatgc
     1681 ggtcatgacg atattcacat cactgtcgca accggagtct tcgggataat ccaaatccaa
     1741 aagcggcacg ccgttcacta cgcctactga tacagcggca acggcttcgc ggatggggtt
     1801 ttcactcaaa atgccgtctg aaaccagttt gccgacggcg atttgcagcg cgacaaacgc
     1861 accggtaatc gaagccgtgc gcgtaccgcc atctgcctga atcacatcgc agtcaatcaa
     1921 gatttgtcgt tcgccgagtt tttccatatc cacgaccgcg cgtagcgaac gcccgataag
     1981 gcgttggatt tcttgtgtgc gcccggactg tttgcccgcc gaagcttcgc ggcgcatacg
     2041 cgaagcggtt gaggcaggca gcatcccgta ttccgccgtt acccagcctt ggtttttacc
     2101 gcgcaaaaac ggcgggacgt tttcatctat ggaagcggta caaatcactt tggtattgcc
     2161 gcattcaata aggcacgaac cgtccgtatg cggcaggaaa tgaggggtaa ttttgatatc
     2221 gcgcaggctg tccgcggcgc gtgaggtgcg gatgtaatca ggcatactgc cctcccgtta
     2281 aaaacagata aattaaaaag cctcaaacat gaaaaactca catttaaggc cttcaaactg
     2341 aaaatttcta cgcttcttcg gctttgctgc ggataatcaa aagcggcagg tggctttggc
     2401 gcattaccgt ttcggcaaaa ctgcccatta aaaggtgcat cagcccggta cgtccgtgcg
     2461 tacccaacac cagcaggtcg gcaccgtttt catcggcata atcaaccaaa tcctgcgcca
     2521 tttcgcgcgc acccttattg gcaaccagca ggtgtttgac ggtattttcc acacccagtt
     2581 cccgggcggt gcgctcggcg gcatccaaaa cctcgttacc ttgtgcaatg gcggctgcct
     2641 cgtaactctc gtgttgcaaa aattcggggg cgagcgccat atattcggca ggattggcaa
     2701 cgtgtaccaa agtcagacgc gcaccgttga ccccggcaag ctcggcggca tgtttcaggg
     2761 cattgatgga cgtttcactg ccgtcaacgg caacaaccag atgtttgtac atatcgtatt
     2821 ctccttttgc accgcctcgc ggtgccctct tgtcggatgg gcgcagggac agtttgcgcc
     2881 gtttcattat agacccgccg tcgggcttta tacaacagcc gaacagcccg accgctttcc
     2941 agtataatat gccgcttccg tgcagtcagg cattttttgc cggctttcgt tcactttttg
     3001 atttgacgca atcttgcagg attcgaccat gtccgacaac gctttgacct cttcgcgacg
     3061 cttcggcggc atcgccagac tctacggaga ctctgccttg gcgcactttt cacaggcaca
     3121 cgtctgcgta gtcggcgtgg gcggtgtcgg ctcgtgggcg atcgaggctt tggcgcggac
     3181 gggcatcgga cgtttgactt tgattgattt ggacaacgtt gccgaatcga atgtcaaccg
     3241 ccagctgcac gccctgaccg gagacttcgg caaagcaaaa gttaccgcct tgcgcgaacg
     3301 cgtcgcccaa atcaacccag gatgccaagt gtttgaaatt gaagatttcg ttaccgaaga
     3361 caatttgccg gaatacttcg gaaaaggttt tgatttcgtc atcgacgcga tcgaccaagt
     3421 gcgcgtcaaa gcagcaatgg cggcttattt tgtggaacgc aaacaaccgt ttgtcctcag
     3481 cggcggcgcg ggcggacaaa aaaatccggc gttaatccaa accgccgatt tgagccgcgt
     3541 aacccacgat ccgctgcttg ccaacctgcg ctacaccttg cggaaacgct acggcttcag
     3601 ccgcgatact aaggaaaaaa tgcgcgtgcc gtgcgtgttc tcaaccgaaa acatcgtacc
     3661 gcctcaatcg ggcgtggcgt gttctgccga tgccgcaccg cagggcttgt cgtgcgccgg
     3721 ctatggcgca agtatgctcg ttaccgcttc gttcggactg tattgcgcac aggcggcggt
     3781 ggaacacatc gcgggcaaaa aataagcaat gccgtctgaa acaggattca gacggcattt
     3841 gaacaaacgc ggcaatcaag ctgtcggttc agaaaaacca acatcggcac gcctaaaacc
     3901 gtattcacaa cctgctcctt ttcaaaacat ttgcatttaa aagccgttat aataccgtct
     3961 gaacatctgc ccgaccacat tatacgtgaa tgtcggcaga ttgttttctt ttgtaaactt
     4021 atattaaaat ccacttaccg attcacgcca tgccgcccat ccctgcccca tccgcaccat
     4081 ccgagcacac tgtcgcatgg gtattcggcc aacccgttac cgatttgccg caggatttgt
     4141 ttatcccgcc cgacgcgctg aaagtcgtat tgggcagttt tcaaggccct ttggatttgc
     4201 tgctctacct tatccgcaag cagaacatcg atgttctcga tattccgatg gtgaagatta
     4261 ccgagcagta tctgcactac atcgcccaaa tagaaaccta tcagtttgat ttggcggcgg
     4321 aatatctttt gatggcagca atgctgattg aaatcaaatc gcgcctgctg ctgccgcgta
     4381 ccgaaaccgt cgaagacgaa gaagccgacc cgcgtgccga gttggtgcgc cgcctgctgg
     4441 cttacgaaca gatgaagctg gcggcgcagg gtttggacgc gctgccccga gccggacggg
     4501 atttcgcgtg ggcttacctg ccgctggaaa ttgccgtcga agccaagctg cccgaagtct
     4561 atattaccga cttgacgcaa gcgtggctgg gtattttgtc tcgggcaaaa cacacgcgca
     4621 gccacgaagt aatcaaagaa accatctccg tgcgcgcgca aatgacggca atcctgcgcc
     4681 gtttgaacgg acacgggata tgcaggtttc acgacctgtt caatcccgaa cagggcgcgg
     4741 cttacgtggt cgtcaacttc atcgcactgt tggagcttgc caaagaaggt ttggtcggaa
     4801 tcgtacagga agtcggtttc ggagaaatcc gaatcagcct caatcatgag ggggcgcatt
     4861 cagacggcat ttccggcaca cggggcgggc gcgatgtgtt ctaatacgcc ccaagccgcc
     4921 accaaaaatc gggagacacg ccatatgacc ggcatcatac attcgctgct tgacaccgac
     4981 ctctacaaat tcactatgct gcaagtggtt ctgcaccagt ttccgcagac gcacagcctt
     5041 tacgaattcc gctgccgcaa cgcctcgacc gtctatccgc ttgccgacat cagggaagac
     5101 ttggaagtcg aactcgacgc gctctgccaa ctacgcttca cccacgacga actcgactac
     5161 ctccgtagcc tgcgtttcat taaaagcgac tttgtcgatt atctcgaact cttccagctc
     5221 caacgccgct ttgtcgaagt cggcacagac gataaagacc gtctgaacat ccgtatcgaa
     5281 ggtccgatga tacaggcgat gttttttgaa atcttcatcc tcgccattgt caacgaactt
     5341 tacttccgcc ggctggaaac ccctgccgtt atcgaagaag gtgaacgccg gcttcaagcc
     5401 aaagccgcgc gcctcaaaga aatcgccgcc gcacaaaacc ccgacgatcc gcccttcctg
     5461 atttccgact tcggcacgcg ccgccgctac aagctcgcgt ggcaggaaca cgtcatccgc
     5521 accctgctcg aagccgcccc aagcatcgtg cgcggcacca gcaatgtctt tctcgccaaa
     5581 aaactcggca ttacccccat cggcaccatg gcgcacgaat tcctccaagc cttccaggcc
     5641 ctcgacgtac gcctgcggaa tttccaaaag gccgcgctcg aaagctgggt gcacgaatac
     5701 cggggcgatt tgggcgttgc cctgaccgac gttgtcggta tggatgcctt cctgcgcgat
     5761 ttcgacctct atttcgccaa acttttcgac gggctgcgcc acgacagcgg cgacccttac
     5821 gtttggggcg acaaagccta cgcccactat caaaagctca aaatcgacag ccgcaccaaa
     5881 atgctgacct tctccgacgg gctggacatc gaacgctctt gggcattgca ccaatatttc
     5941 aaagaccgct tcaaaaccgg cttcggcatc ggcaccaacc tcaccaacga tatggggcat
     6001 acgcccttga atatcgtctt gaaactggtc gaatgcaacg ggcagtccgt cgccaagctg
     6061 tccgactctc cgggcaaaac catgaccaac aacagcacct tcctcgccta cctgcgccaa
     6121 gtgttcgacg tacccgaacc cgaaacgccg taaaccggca gaaaaagcgc acaattcctg
     6181 tttctgccgc ataaaatctt ttaaaatacc gcctgatttg aatttaaccg aaagaccgaa
     6241 cttcatgaac ctacatcaaa ccgtcgaaca cgaagccgcc gccgcctttg ccgccgcagg
     6301 catcgccgac agtcccgttg ttttgcagcc gaccaaaaat gccgaacacg gcgatttcca
     6361 aatcaacggc gtgatgggtg cggcgaaaaa agccaaacaa aacccgcgcg aactggcgca
     6421 aaaggtcgcc gacgcattgg caggcaacgc cgtcatcgaa agcgcggaag tcgccggtcc
     6481 cggttttatc aacctgcgcc tgcgccccga atttctcgcg caaaacattc agacggcctt
     6541 gaacgacgca cgtttcggcg tggcaaaaac cgacaaaccg caaaccgtcg ttatcgacta
     6601 ttcttcgccc aatctggcga aggaaatgca cgtcggccac ctgcgttcca gcatcatcgg
     6661 cgacagcatt tcgcgcgtgt tagaatttat gggcaatacc gttatccgtc aaaaccacgt
     6721 cggcgactgg ggtacgcagt ttggcatgtt ggtcgcttat ttggtcgagc agcaaaaaga
     6781 caatgccgcg ttcgagttgg cggatttgga gcagttttac cgcgccgcca aagtgcgctt
     6841 tgacgaagac cctgcctttg ccgacaccgc acgcgaatac gttgtgaagc tgcaaggcgg
     6901 cgatgaaacc gttttggcat tgtggaaaca gtttgtcgat atttcgctct cgcacgccca
     6961 agccgtttac gacacgctgg gcttgaagct gcgccctgaa gacgtggcag gcgaatcgaa
     7021 atacaacgac gatttgcaag ccgtggtcga tgatttggtt caaaaaggct tggcggtcga
     7081 agacgacggc gcgaaagtcg tgttcttgga cgagtttaaa aacaaagaag gcgaacccgc
     7141 cgcatttatc gtgcaaaaac aaggcggcgg cttcctttat gcttccaccg atttggcgtg
     7201 tctgcgctac cgtgtcggca cgcttcatgc cgaccgcctg ctgtacgtcg tcgaccaccg
     7261 ccaagccctg cactttgagc aactctttac cacttcacgc aaagccggct atctgccgga
     7321 aaacgtcggc gcggcatttg tcggcttcgg cacgatgatg ggcaaagacg gcaaaccgtt
     7381 caaaacacgc agcggcgaca ccgtgaaact ggtcgatctg ctgaccgaag ccgtcgaacg
     7441 cgccaccgct ttggtgaaag aaaaaaatcc cgaattgggt gcagacgaag cggcaaaaat
     7501 aggcaaaacc gtcggcatcg gcgcggtcaa atacgccgac ttgagcaaaa accgcaccag
     7561 cgattatgtg ttcgactggg acgccatgct ctcgtttgaa ggcaacaccg ccccctatct
     7621 gcaatacgcc tacacccgcg tgcaaagcgt gttccgcaaa gcaggcgaat gggacgcgga
     7681 cgcgccaacc gttttgaccg aacctttgga aaaacaactg gccgccgagc ttttgaaatt
     7741 tgaaaacgtg ctgcaaagcg tggcggacac ggcgtatccg cactacctcg ccgcctacct
     7801 ctatcaaatc gctaccttgt tcagccgctt ctacgaagcc tgcccgattt tgaaagccga
     7861 aggcgcaagc cgcaacagcc gcctgcaact ggcgaagctc accggcgaca cgctgaaaca
     7921 aggcttggat ttgctgggca tcgatgtgtt ggacgtaatg taaaaccgca ccgcccgatt
     7981 gcggacaaca gccccgcaat caaccggcgg accatcctta tccgaatctg aaaaaagcgg
     8041 cgcgatacac cgtatccgcc gcctcccaaa atgcgaaaca gacaaacgcc aagcaagcaa
     8101 gcaagcaagc aagcaagcaa gcaagcaagc aagcaaaaaa ttataacccc cttcctgccg
     8161 acgcacgcac tttccgcgcg gcgcgttccc cttttcccgc tcctcaaacc cgccttttct
     8221 tcaggcaggg tttcagcccg cctcctttcc ctgttttcat tccctcaaca cgcgtgcgcg
     8281 ccccctgccg caccgtgccg cactttcgcg cccggacggc atcggtctgt catccggttc
     8341 tctgttttaa atacccctgt ttcagaaaga aattgcagat gtttcaacac acaggacgac
     8401 acataaagca ccgccctatg tgttgccctg atttggaagg ggttgcgccc ctcccaaata
     8461 aagtctgatc ctgccgcccc gaaggacaga tgtccgagtg gcggggtttc aaccgaaaag
     8521 gaaatacgat gaatattcac gccctgctct ccaaacaatg gacgctgccg ccattcctgc
     8581 cgaaacggct gctgctgtcc ctgctgatac tgctggcccc caatgcgatg ttttgggttt
     8641 tggcactgct gaccgccacc gcccgcccga ttgtcaattt ggactacctt cccgccgcgc
     8701 tgctgatcgc cctgccttgg cgtttcgtca aaattgccgg cgtattggcg ttttggctgg
     8761 cggttttgtt tgacgggctg atgatggtga tccagctttt cccttttatg gacctcatcg
     8821 gcgccatcaa cctcgtcccc ttcatcctga ccgcccccac cccttatcag ataatgaccg
     8881 ggctgttgct gctgtatatg ctggcgatgc cgtttgtgtt gcagaaagcc gccgccaaaa
     8941 ccgacttccg gcacattgcc gtctgcgccg ccgttgtggc ggcagccggc tatttcaccg
     9001 gccatttgag ttactacgac cgggggcgga tggccaatat cttcggcgca aacaacttct
     9061 attacgccaa aagtcaggcg atgctctaca ccgttagcca gaatgccgac tttattaccg
     9121 ccggcctggt cgatcccgtc ttcctcccct tgggcaatca gcagcgtgcc gccacgcatc
     9181 tgaacgagcc gaaatctcaa aaaatcctct ttatcgtcgc cgaatcttgg gggctgccgg
     9241 ccaatcccga acttcaaaac gccacttttg ccaaactgct ggcgcaaaaa gaccgttttt
     9301 cggtttggga aagcggcagt tttcccttca tcggcgcgac ggtcgaaggc gaaatgcgcg
     9361 aattgtgcgc ctacggcggt ttgcgcgggt tcgcactgcg ccgcgcgccc gacgaaaaat
     9421 ttgcccgctg cctccccaac cgtttgaaac aagaaggtta cgccaccttt gcgatgcacg
     9481 gcgcgggcag ttcgctttac gaccgcttca gctggtatcc gagggcgggc tttcaagaaa
     9541 tcaaaaccgc cgaaaacctg atcggtaaaa aaacctgtgc cattttcggc ggcgtgtgcg
     9601 acagcgagct gttcggcgaa gtgtcggcat ttttcaaaaa acacgacaag ggactgtttt
     9661 actggatgac gctgaccagc cacgccgact atcccgaatc cgacattttc aaccacaggc
     9721 tcaaatgcac cgaatacggc ctgcccgccg aaaccgacct ctgccgcaat ttcagcctgc
     9781 acacccaatt cttcgaccaa ctggcggatt taatccaacg ccccgaaatg aaaggcgtgg
     9841 aagtcatcat cgtcggcgac catccgccgc ccgtcggcaa cctcaatgaa accttccgct
     9901 acctcaaaca ggggcacgtc gcctggctga acttcaaaat caaataacaa caatgccgtc
     9961 tgaacgcacc aacagccttc agacggcatt ttgcagacag accgaccctt caagcccact
    10021 tttttcatca tctccgataa attgc
//
LOCUS       DAXHKX010000056        10024 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHKX010000056 DAXHKX010000000
VERSION     DAXHKX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.056"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            251..1150
                     /gene="crgA"
                     /locus_tag="ACFMWZ_001089"
     CDS             251..1150
                     /gene="crgA"
                     /locus_tag="ACFMWZ_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC5935062.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1374..4589)
                     /gene="carB"
                     /locus_tag="ACFMWZ_001090"
     CDS             complement(1374..4589)
                     /gene="carB"
                     /locus_tag="ACFMWZ_001090"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC5935063.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VNTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4596..5144)
                     /locus_tag="ACFMWZ_001091"
     CDS             complement(4596..5144)
                     /locus_tag="ACFMWZ_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935064.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5147..5710)
                     /locus_tag="ACFMWZ_001092"
     CDS             complement(5147..5710)
                     /locus_tag="ACFMWZ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC5935065.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6558..6947)
                     /locus_tag="ACFMWZ_001093"
     CDS             complement(6558..6947)
                     /locus_tag="ACFMWZ_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC5935066.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7520..7645)
                     /locus_tag="ACFMWZ_001094"
                     /pseudo
     CDS             complement(7520..7645)
                     /locus_tag="ACFMWZ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7699..8832)
                     /gene="carA"
                     /locus_tag="ACFMWZ_001095"
     CDS             complement(7699..8832)
                     /gene="carA"
                     /locus_tag="ACFMWZ_001095"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC5935067.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2295 a         2571 c         2563 g         2595 t
ORIGIN      
        1 taaaatattc atttgttacc tcgtttgtga attgatgaat gtattgtgcc gttttacttt
       61 ataatttaaa agtgcaaaaa taagaaaaca cttttgcgtc aaatgaaata atcagatgtt
      121 ccaacacacg ggatggcaca taaagcaccg ccctgtgtgc cgtcctgatt cggaaggggt
      181 tacacccctc ccaaataaag tctgatcctg ccgccctaaa gggcggggtt ccaaccgaaa
      241 aggaaacacg atgaaaacca attcagaaga actgaccgta tttgttcaag tggtggaaag
      301 cggcagcttc agccgtgcgg cggagcagtt ggcgatggca aattctgccg taagccgcat
      361 cgtcaaacgg ctggaggaaa agttgggtgt gaacctgctc aaccgcacca cgcggcaact
      421 cagtctgacg gaagaaggcg cgcaatattt ccgccgcgcg cagagaatcc tgcaagaaat
      481 ggcagcggcg gaaaccgaaa tgctggcagt gcacgaaata ccgcaaggcg tgttgcgcgt
      541 ggattccgcg atgccgatgg tgctgcatct gctggcgccg ctggcaacaa aattcaacga
      601 acgctatccg catatccgac tttcgctcgt ttcttccgaa ggctatatca atctgattga
      661 acgcaaagtc gatattgcct tacgggccgg agaattggac gattccgggc tgcgtgcacg
      721 ccatctgttt gacagccgct tccgcgtaat cgccagtcct gaatacctgg caaaacacgg
      781 cacgccgcaa tctacagaag agcttgccgg ccaccaatgt ttaggcttca ccgaacccgg
      841 ttctctaaat acatgggcgg ttttagatgc gcagggaaat ccctataaga tttcaccgca
      901 ctttaccgcc agcagcggtg aaatcttacg ctcgttgtgc ctttcaggtt gcggtattgc
      961 ttgcttatca gattttttgg ttgacaacga catcgctgaa ggaaagttaa ttcccctgct
     1021 cgccgaacaa acctccgata aaacacaccc ctttaatgct gtttattaca gcgataaagc
     1081 cgtcaatctc cgcttacgcg tatttttgga ttttttagtg gaggaactgg gaaacaatct
     1141 ctgtggataa ttctataaaa acctgtcata aaattgtttt tactcactta ataactttga
     1201 gaaaaaataa ataagatgat gtatagccgc ggcaatggca gtctatatgg ggagcgtgat
     1261 agggctgttc cttatgtacg gcttgtgtgt ggcaacatcg gctttccaaa tgacaaaatg
     1321 ccgtctgaaa ccgcaaaacg gcttcagacg gcattttcaa cctgattcag gcatcagttg
     1381 cggtttttca aacgcccgtg caactcttga acgctgtaca cgcccagatg gtctcggctt
     1441 ttcgcgcctt cgctcatcgc ttcgccgccg gcggtggtgg tgtattgcgg cacgcgttgc
     1501 tgcaatgcgc tttggcggat gatgtggctg tcggacacgg attgcggatc gctggaaacg
     1561 gtgttcacga ccagtgcgat ttcgccgttt ttcagcgcat cgccgatgtg cgggcggcct
     1621 tcggggactt tgttgatggt ctgcacaatc agcccgtgtt cggtcaggta ttgcgccgtg
     1681 ccgcgcgtgg cgcagatgcc gtagcctaaa acttggaagt ttttagcggt tttaatgacg
     1741 cgttctttgt cttcttcgcg cacggagagg aagattttgc cggtcgggtt gaggcgttcg
     1801 cctgcgccga gttgggcttt gtagtaggct tcgccgaagc ttgcgcccac gcccatgact
     1861 tcgccggtgg agcgcatttc cggtcccaaa atcgtgtcca cgcccgggaa tttgatgaat
     1921 gggaacacgg cttctttaac ggcatagaaa tcggggacaa cttctttttc cacgccttgt
     1981 tctttcaggg aaatacctgc catgcagcgt gcgccgactt tggcgagcgg cacgccggtg
     2041 gctttggaga cgaagggcac ggtacggctg gcgcgcgggt tcacttccaa cacgaacacc
     2101 acgccgtcct gcacggcaaa ctgcacgttc atcagaccga ccacgcccag cgcgtacgcc
     2161 atcgctttgg tttggcggcg gatttcgtct tggatttctt cgcttaagga gtagggcggc
     2221 agcgagcagc cagagtcgcc ggagtgaatg cctgcctgtt cgacgtgctg cataatgccg
     2281 ccgataacca cgtctttgcc gtctgaaacg cagtccacat ccacttcaat cgcgttgttg
     2341 aggaagaaat cgagcaacac ggggctgtct tcggaaacct gcacggcttc gcgcatgtat
     2401 ttttgcaagg cttcggcaga gtggacaatc tgcatcgcgc gtccgcccaa aacataagac
     2461 gggcgcacga ccagcggata gccgatttct tcggctttga cgagtgcttc ttcttcgttg
     2521 tgggcgatgc ggttgggcgg ttggcgcaga cctaagtcgt tcaacacttt ttggaagcgt
     2581 tcgcggtctt cggcggcgtc gatgctgtcg gcggacgtgc cgatgatgtt tacgccgttt
     2641 tcaaccaacg cgttggcgag tttcagcggg gtttgaccgc cgtaatgcac aatcacgccc
     2701 cacgggtttt cggtgcggac gatttccaac acgtcttcca atgtcagcgg ctcgaaatag
     2761 aggcggtcgc tggtgtcgaa gtcggtggac acggtttctg ggttgcagtt gaccatgatg
     2821 gtttcaaagc ccgattcgcg cagggcgagt gcggcgtgaa cgcagcagta gtcaaactcg
     2881 atgccctgac cgatgcggtt cgggccgccg ccgagaatca tcactttttt acggtcggaa
     2941 ggacgggatt cgcattcctc ttcgtaagtg gagtagaggt aggcggtttc ggtggcaaac
     3001 tcggcggcac aggtgtcgac gcgtttgtaa accggatgca gcttcagcgc gtagcggtgt
     3061 tcgcgaactt ctttttcgct tacgttcaac aattgtgcca aacgtttgtc ggagaagcct
     3121 ttgcgtttca gacggcgtag ggcggcgaaa tccaaatcac tcaaaatgcc gtctgaaacc
     3181 gctttttctt ccttcatcaa gtcttcgatt tgcgccagga accaagggtc gatggcgcag
     3241 atttcgtgga tttcttccag cgtgaagccc gcgcggaacg cgtctgccac aaacagcata
     3301 cgttcggggc cggggtttgc cagttcgcgg cggatttccg ctttgtcgga gcttctcgga
     3361 ttgaagccgc acaagcctgt ttccaagccg cgcagggctt tttggaagct ttcctgaatc
     3421 gtgcggccca tcgccatcac ttcgcccacg gatttcatct gcgtggtcag gcggtcgtct
     3481 gcggcaggga atttttcaaa tgcgaaacgc gggattttgg ttaccacata gtcgatggaa
     3541 ggctcgaacg acgcgggggt tttgccgccg gtgatgtcgt tgcgcaactc gtccagcgta
     3601 aagccgaccg ccagttttgc cgccaccttc gcaatcggga agcccgtcgc tttggaagcc
     3661 agcgcggacg aacggctcac gcgcgggttc atctcaatca caatcatctc gccgttttca
     3721 gggttcaccg caaactgcac gttcgagccg cccgtgttca cgccgatttc gcgcaatacc
     3781 gccaacgaag cgttgcgcat gatttggtat tctttgtccg tcaacgtttg cgccggcgca
     3841 accgtaatcg agtcgcccgt atgcacgccc atcgggtcga agttttcaat cgagcagatg
     3901 atgatgcagt tgtcgttttt atcgcgtacg acttccatct cgtactcttt ccagccaagg
     3961 acggactgct caatcagcag ctcgtgcgtg ggcgaggcat cgaaaccgcg ttcgcaaatc
     4021 gccaaaaact cgtctttatt gtaggcaatg ccgccgcccg aaccgcccat ggtgaaagaa
     4081 ggacgaatca gggtagggaa gccgacctgt tcttgcgccg ccaaagcttc gttcatggtg
     4141 tggcagacaa aagatttcgg gcaagataaa ccgatttttt ccatcgcctc cttaaagcga
     4201 ccgcggtctt ctgctttgtc gatggcgtct tccgtcgcac cgatcaactc gacattgtat
     4261 ttcgccagca cgccgttgcg cgccaaatcc agcgcacagt tcagcgcggt ctgaccaccc
     4321 atcgtgggca gaatcgcatc gggccgctcc ttggcgataa tcttctccac cgtctgccac
     4381 ataatcggct cgatgtaggt aacatccgcc atttcagggt cggtcatgat ggtggcgggg
     4441 ttagaattca ccagaatgac tttatagcct tcttcacgca aagccttgca ggcctgtgcg
     4501 cccgaatagt caaattcgca ggcctgaccg ataacgatag ggccggcgcc gatgataagg
     4561 atggatttta ggtcggtacg tttgggcatg ggtggttact cttgaattaa agaaggttgt
     4621 tttgttacat agcttttaag aaaagtattt cccttctcta aaattgaagc taaaacatca
     4681 tcttctatgt tagataaaat attctctaaa tctgcgcttg atttaatttt aaagaattct
     4741 tttgtttgag aatggtatac aagagaaggt ttatttagct gtaaaagttc tgctatatta
     4801 tttaatgaac caatactctt accatcccat aaaataaagc cataatcagc aatttctgcc
     4861 attttcttat cttttgctgt ataaaatacc cttccagttc ctttgctgtc tacttggaca
     4921 aactgccaat tccccacatt atttcggtaa attttgccag aaaagtaaat atgcacatta
     4981 gcataatctt gttcttgtag aaatttttgg atagctttat cagccccatt agcatctcca
     5041 ataacaatat caaaattatt gctaagaata ttgttaattc tttctcgtat ttgtggattt
     5101 agtcgtgaaa tgctgcgtga accagaaaag aaaatagtgc gcatttttat cctctagtct
     5161 ttgtcattgc taaaacaatt acagatctag cattttttga aaacaatttt tcagttgata
     5221 tttccaatgt tgctccgcta tcaaataaat catcaattac taaaatattc ttattggata
     5281 gatctatatt gtcaattgtg atggaattat tcagtatttc tagtttttct gatttttctt
     5341 caatattttt gagtggtgtg tgtgagctag attttcttaa aataggagaa tatggaatat
     5401 tcaagcgatc acttagttct ttggcaatta attgaactgg ttgattaata cgctctgttg
     5461 taaaaggggc gggaacaatt aaatttatag attctaaacc actaaatttt tgtaaaatat
     5521 agtctaccag caaagaaaca ttttgggttt gattacgata ttttaattga taaacccatt
     5581 ctccaatcac acttctcttg ctatcaaagt gaggatgccc taattcatca taacctaaca
     5641 aaatactttt ttgcatatga tggtctaaag caaatccttt tgtccagtta ccatttagtt
     5701 ctatcgccat ttcattgact ccttgtcaaa attaagtata acgagcaatc ctagcaagcc
     5761 gtagctcgca tgcagggtgt gtgcggtacg cacgcgttct ccattttccc tgcaacccca
     5821 acccaccgcg tgcgtgcctt gcggcacact ctctgccgat gagttcaaag gtcgtctgaa
     5881 aaaacgctcg cggcgtttgt tccacctgcc ttgcgataaa ggctgcctga aaaattcccg
     5941 ctgttgatat ttcgggcatt ctttctcaaa ccgcctgcaa ccgcaaaatc tgctgaaacc
     6001 tactccctcc cccgtggggg agggttgggg agagggcatt ccccgaattt cggcaacctt
     6061 tcccaacaac tcaaccaccg aaatacaagc cttgtggctt gttgccctct ctctagctct
     6121 ctcccacggg agagaggacg gagtggctgt agggctaagg gttctgcaag gaaacaggtt
     6181 gtgcaggttg ctttttgctt ttcagacgac ctgaaatcaa cggtaaatcc aagtcgcttt
     6241 tcaaaccgcc tgcaaccgca aaatccgctg caacctaccc cctcccccgt gggggagggc
     6301 tggggagagg gcattctccg aatttcggca acctttccta acactctaac cgccaagaca
     6361 caagccttgc ggcttgttgc cctctctcta gctctctccc acgggagaga ggatggggtg
     6421 gctgttgggg ctaaggtttc tgtaaactaa tcaggttatg caggctgctt tttgtttttc
     6481 agacggcctt tataaatgct caattcagtc ggctttatcc tattattttc aataatccaa
     6541 tcaaaatcag ccatctgcta tcgtgcagct tgcttttcca attcttgcaa cacgagcagg
     6601 atttctgtca gcacatcgtt tgtctgctgt aaaatctcgt gattccaaaa ccgcagtacg
     6661 gtaaagccca agctgttgag atatgccgtc cgcgcgtggt cgtatgcggc ttgttctgtg
     6721 tgctgccctc cgtctgcttc gacaatcaac ttgggcgtta cgcacataaa atcaacaata
     6781 tagtttccca tcggctgctg gcggcggaat ttatagccgt tcagacgacc tgcccgcagg
     6841 tgctgccaca attttgcttc cgccccgctc atttcttggc gcatggcttt ggcgcgttgg
     6901 cgcaagatgg ggttgtcggt ggtcaagagt ttctcgtgcg ggttcatttt gttgtcgtgc
     6961 tgtttgctgc cctctctcta actctctccc acggggagag aggacggggc ggttgttggg
     7021 gttaaagggg ctgtaaggaa aacaagtcgt tcaggccact ttttactttt cagacgacct
     7081 gcaatcaacg gttgtaaatc caagttgctt ttcaaaccgc ctgtaaccgc aaaacttgct
     7141 gcaacctacc ccctcccccg tgggggaggg ctggggagag ggcattcttc gaatttcggc
     7201 aaccttcccc aacaccctaa ccgcccaaac acaagccttg ctgtttgttg ccctctctct
     7261 aacgctttcc cacagggaga caggacgggg cggctgtagt gattaagggt tttgcaagga
     7321 aaacaaggaa aacaagttgt acaggctgtt ttttattttt caagcgacct gaaatcaacg
     7381 cttgtaaatc caagtcgctt ttcaaactgc ctgcaaccgc aaaatctgct gcaacctacc
     7441 ccctcccccg tgggggaggg ctggggagag ggcattttcc aagttgcggc aatctttccc
     7501 aaatccctta gtcttctaaa atacaagcct tacggttgtt gccaaatgaa acttcttcca
     7561 tacccaaaac ttgcgtgtaa aacgcgatgg ttcggtcaat gtcggcaacg gtcagtacca
     7621 gatggtcgag tgcgctaatc ttcatttcca gctttgtttt tcagacggca gcagcatact
     7681 gttgccgtct gaaagccatt atgcccgttt tgccgccttc atattgccaa tgaatttgtc
     7741 aaacaaatag ccgacatctt gcggaccggg gctggcttcg gggtggcctt ggaagcagaa
     7801 cacaggtttg tcggtcagct cgatgccttg caaagtgttg tcaaacaagg atttgtgggt
     7861 aatgcgtgcg ttagcgggca gggtgtcggc atcaacggca aaaccgtggt tttggctggt
     7921 gatgacgact ttgccgctgt ccaaatcttg cacagggtgg ttcgcaccgt ggtggctgaa
     7981 gcgcattttc agggttttcg cgccgatagc gaggctgatg agctggtgtc ccaagcagat
     8041 gccgaaaatc ggtttgccgc tttccatcag tttttgcacg gcttcgatgg cgtaggtgca
     8101 aggctcgggg tcgccggggc cgttggacag gaacacgccg tcgggattga gtgccaacac
     8161 gtcttgtgcg ctcgtttggg cggggacgac ggtcaggcgg cagccgcgcg aggcgagcat
     8221 acgcaggatg ttggttttca cgccgaaatc gtaggcgacg acgtggtaag gctgttcgtc
     8281 aggggtaacg aaacccttgc ccaattccca ttcgccttcc gtccattcgt aagtttccgt
     8341 gcaggaaact tcttttgcca agtctttgcc gaccatgctg ccgaacgcgg cgatgagttc
     8401 ttgcgctttt tcgattgtgg catccgcacc ggtcagaatc gcgccgcctt gcgcgccttt
     8461 ttcgcgcaac agcgtggtca ggcggcgggt atcgatgtcg gcgatggcga cggttttgtt
     8521 gcgtaccaga tagtcgtgca ggctttcgga ggcgcggaag ttgctgtgca agagcggcag
     8581 gtcgcggata atcaggccgg cggcataaac gctgcggctt tcttcatctt cggcgttggt
     8641 gccggtgttg ccgatgtgtg ggtaggtgag ggtaacgatt tgtttgcagt aggacgggtc
     8701 ggtcaggatt tcctgatagc cggtcatcga agtattgaac acgacttcgc cggaagtcga
     8761 accttcgtaa ccgattgatg tgccgtgaaa tacgctgccg tcagcgagga cgaggagggc
     8821 gggggtgctc atgatgggaa tcctgttttt aataaaattc ttgacaatgc cgtctgaagg
     8881 gacttcggca gggggcatcc ggtcggaaaa atccggtcaa aaaaaaacac gccgcgcaag
     8941 atagacgcgt aacgtgcttt tcgggcaagt gcctataccc tgaaagcagc atattttaag
     9001 acgaaacata cccgatttca agcgcaaacg cggcaggcgg gctgatatgt tcgcgcgaaa
     9061 acggaaatcc ggggcataaa accgcccgct ccgcgcccac tatgcccgtt acgcatttcc
     9121 ccacgtccgc ccggcgaaac tatgggaata cccgaatcgt caggaatacc tgaatcgtca
     9181 ggaatacccg aaccgtcaga aatacccgaa ccgtcattcc cgcgcaggcg ggaatccaga
     9241 cttgtcggca cagaaactta tcgggtataa cggtttcttg agattttacg tcctagattc
     9301 ccgcctgcgc gggaatgacg gcatcggttt gacggtattt aattgaattg cggaaattga
     9361 cggattcagt gagattggcg ggatgaagcc taccctatag cccgcctttt atagcggatt
     9421 aacaaaaacc ggtacgggtt gccccgcccc ggctcaaagg gaacgattcc ctaaggcgcc
     9481 caagcaccag gcgaaccggt tccgtaccat ttgtactgcc tgcggcccgc cgccttgtcc
     9541 tgatttttgt taatctgcta tacgaacccg ccccctcccg aaaaacgcaa aaaatgccgt
     9601 ccgaaaacct ttcggacggc attttcgcgt gaaccgtcat tcccacgaaa acagaaaacc
     9661 aaaatcagaa acctaaaatt cgtcattccc acgaaagtgg gaatccagga ctcaggacct
     9721 gagaaacctt tttacccgat aagttcccgt accgaccggt ccggattccc gcctgcgcgg
     9781 gaatgacgcg tttcatttgc cgccccctcc cgaaaaacgc aaaaaatgcc gtctgaaagc
     9841 tctgtaggga gggcttcagc ccaccgattc catcaatccc acatttccgc cgttttcacc
     9901 gaatccgccc cttttcacaa tccagtccta cgcaactgaa ccgtcattcc cgcgcaggcg
     9961 ggaatccgga cttgtcggca cggaaactta tcgggtacaa cggtttcttg agattttacg
    10021 tcct
//
LOCUS       DAXHKX010000057         9923 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHKX010000057 DAXHKX010000000
VERSION     DAXHKX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.057"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..559
                     /locus_tag="ACFMWZ_001096"
     CDS             <1..559
                     /locus_tag="ACFMWZ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC5935068.1"
                     /translation="IHPRVSVGYDFGGWRIAADYASYRKWKESNFSTKKVTKEINDNY
                     QETKTEHQGNGSFHASSSLGLSAIYDFKLNDKFKPYIGARVAYGHVKHQVHSVETKTT
                     TVTSKPKGKNLKGGPVSLQHSSQPAYHESNSISSLGLGVIAGVGFDITPKLTLDTGYR
                     YHNWGRLENTRFKTHEVSLGMRYHF"
     gene            complement(533..742)
                     /locus_tag="ACFMWZ_001097"
     CDS             complement(533..742)
                     /locus_tag="ACFMWZ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935069.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGVGESEVVAHAQ"
     gene            complement(760..1317)
                     /locus_tag="ACFMWZ_001098"
     CDS             complement(760..1317)
                     /locus_tag="ACFMWZ_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC5935070.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1393..3027)
                     /locus_tag="ACFMWZ_001099"
     CDS             complement(1393..3027)
                     /locus_tag="ACFMWZ_001099"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC5935071.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKEFLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3097..3430)
                     /locus_tag="ACFMWZ_001100"
                     /pseudo
     CDS             complement(3097..3430)
                     /locus_tag="ACFMWZ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3430..4536)
                     /gene="serC"
                     /locus_tag="ACFMWZ_001101"
     CDS             complement(3430..4536)
                     /gene="serC"
                     /locus_tag="ACFMWZ_001101"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC5935072.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4901..5332
                     /gene="rimP"
                     /locus_tag="ACFMWZ_001102"
     CDS             4901..5332
                     /gene="rimP"
                     /locus_tag="ACFMWZ_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC5935073.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            5360..6862
                     /gene="nusA"
                     /locus_tag="ACFMWZ_001103"
     CDS             5360..6862
                     /gene="nusA"
                     /locus_tag="ACFMWZ_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC5935074.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNIGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6875..9763
                     /gene="infB"
                     /locus_tag="ACFMWZ_001104"
     CDS             6875..9763
                     /gene="infB"
                     /locus_tag="ACFMWZ_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC5935075.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPPAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLHIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
BASE COUNT         2671 a         2850 c         2487 g         1915 t
ORIGIN      
        1 catccacccc cgggtgtcgg tcggctacga tttcggcggc tggaggatag cggcagatta
       61 tgccagttac agaaaatgga aagaaagtaa tttttctact aaaaaagtta ctaaagagat
      121 aaacgacaac taccaagaaa ccaaaacaga acatcaagga aacggcagct tccacgcctc
      181 ttcttctctc ggcttatccg ccatttacga tttcaaactc aacgataaat tcaaacccta
      241 tatcggcgcg cgcgtcgcct acggacacgt taaacatcaa gttcattcgg tggaaacaaa
      301 aaccacgact gttacctcta aaccaaaagg aaaaaatcta aagggaggac ctgtctcatt
      361 acagcattcc agccaacctg cctatcacga aagcaacagt atcagcagct tgggtcttgg
      421 tgtcatcgcc ggtgtcggtt tcgacatcac gcccaagctg accctggaca ccggataccg
      481 ttaccacaac tggggacgct tggaaaacac ccgcttcaaa acccacgaag tctcattggg
      541 catgcgctac cacttctgat tccccgacac cgatgccgtc tgaaccttca gacggcatga
      601 gacctttgcc tgcgtacttg gtacgctggt cgcctccgaa catggcgcga cacccgacat
      661 ttccgccgaa cgcatcgggc gtttcatgaa tccggtttaa aacgcatgga aaaatgccgt
      721 ctgaaagcct ttcagacggc atttcgcttg agattccgtt taccaatggc tgacaaacgc
      781 ttccaaatcg gtattcttgg gcttatgcac ttcctctgtc ggcgtgccga ccatcatcag
      841 cccgatgatt ttatccttat ccgcacatcc gaaagcctca cgcaacagag ggctgttgac
      901 ccacatcccc gttatccaga cattgtcgaa accttgcgcc gttgccgcca gttgcagcgc
      961 atacgccgca caacccgccg tcagcatctg ctcccattcc ggtttcggct taggcacatc
     1021 gcggttcggc gcaaacgtta ccccgataac catcggcgcc atattgccca ctttttccgc
     1081 ctttttcatc gcatcgtcgc cgaaattcaa ttcggcaacc gtttgcttta acacatcgcg
     1141 aaaacgttgc aatcctacct cgccttgaat cacggtaaaa cggaaggggc gcatattgcc
     1201 gtgatcggga acttgggttg ccgcctgaaa tatttgttcc aactccgccg catcgggggc
     1261 ggggtgcttc agctttttgg aagatcggcg gttcgtcaat aattttaaag catccatatc
     1321 gttatactcc ggtcatcggt cgggtgttcg gacacatatg ccgtccgaag gcttcagacg
     1381 gcatatccgg catcagcgcg gacggcggca ggctgccaat atatccattt ccttccgata
     1441 ggtttggcta ttggaaatgt ccatcagccc caataccgtg ctgaaatagt ggtcgtgcga
     1501 atattcgttt tccgccgctt tttgtttgag gcattggaaa tctatgcccc cgtgttggcg
     1561 gaaggctttg gaaaaccaca taaccatcgg gatatgcgtc tgcccggaag gcgcgatggc
     1621 gtaaggcgcg gcgtgcaggt acatcccgtt ttcgcccaaa ctttcgccgt ggtcggaaac
     1681 ataatgcacc gcgctttcca aatcgtcgcg gttttcaagt ttgcggataa ccttgtcgat
     1741 aaactggtcc acatacaaaa ccgtattgtc gtaagtgttg accagcgtgg cgcgggtgca
     1801 tttgttgatt tcgttggtgt cgcaggtcgg cgtgaatttg cgttcggctt cggtatagcg
     1861 ttcgtaatac gtcggcccgt ggctgccgat ggtatgcagg attaaaaccg cgtctttatc
     1921 gtttttgttg aggacttcgt cgaacttggt cagcaggata ttgtcgagac actcgccgtt
     1981 gcggcagtat tcgggcaggt tgagcgaggt aacgtcggta ttcggcactt tgccgcacac
     2041 gcccttgcag ccggaatcgt tttccaacca ggtaacttcc acgccggcgc gctgcacgat
     2101 gtccagcagg ttgtcttggt gttcggcttt gatttcgtca taatccgtgc ggtcgaaggt
     2161 tgagaacata cacggcaggg agtgcgcggt cgatgtgccg cagcttctga cctgcgggaa
     2221 attgacaatt tcatcgccgc gcgcggcaag cagcggcgta gtttggcggc tgtaaccgtt
     2281 caaaccccag ttggcggcac gcgtggtctc gcccacgacc agcaccacga aacggcgcag
     2341 gctgccggcc ggccggtttt gcacgaccgc catatccaat tgcgtataag gaatattgga
     2401 acgcttccaa tctttgtatt tcgacacgcc cgcgccgatg aaattagacg gcacaatcag
     2461 atgggttact gatttattgt tgcggaaaaa cgaggcgtaa tcctgatatt gcaacattgc
     2521 gatgcccaac gcgcacaaaa aggaaacggc ggcaagcaca aggcgcgtca aaaactcctt
     2581 ataccaaacg cggtatttaa ccttgacggc gatatacgcc agcgcgggca atacgcccaa
     2641 acatacaatc cacagcacat agcccggcgt aatcaggcgc gcgctttcgg cagccgtagt
     2701 ttgcaagaca ttattcaaca tcgacttgtt gaaatagata ttgaaaaata tttcttggta
     2761 agacaccgcc gcactgataa ccaatatcaa cggaatcaat accttatgca cgaaaggcag
     2821 ggcaatgacg tgaaaaacga aattacttaa aaaaaacagc accaccggca tcgtatagag
     2881 gaagatatcc gccccggtgc cgttaaaagg atgaagctcg acaactttgg caaaaaaggc
     2941 gtaattcaat accagcgagg aatacaggga aaggaaggca atcagcgcgg aagagccgag
     3001 cttcggcctc aggttcggtt ttatcatttg gaatgtgtcg gataagggtt ggaaaaggca
     3061 tccggcattt ggaatccgga ttattgaaaa agattcttaa ttataaggca acggagcaaa
     3121 gcagggcaag aaaacggcgg ctgtgcgggg ggttccgccc gccattcaaa cgtccggcag
     3181 acataaaaac atcgtaagca agattcgaac cggtctgcaa ccgcccctgc cagaaaaacg
     3241 ggcaaagcat ttcatattgg aaaaacccgg ccgcgccgcc gacgggacag tccggcacaa
     3301 acagcatcac gctcggattg aaaaggacgg atagccgccg gcagcacaat cttaccacct
     3361 ccaaaacgcc gccggagaac gccgaaacag ccgatgccgt ctgaagccgc ttcagacaac
     3421 atcgggacat caaccgtaac gccgttggaa atcgcgcata aaatccgcca aagcccgcac
     3481 gccttcaagc ggcatcgcat tataaatgct ggcacgcata ccgccgacgg ttttatagcc
     3541 cttaagcagg cacaagccct gcaattcggc ttccagcaca aaacggcggt caagctcctc
     3601 atcccccgtt tggaacacga cattcatttt agaacgcgca ttcggacgga tacggttgat
     3661 atagaaaccg ccgctgccgt ctatcgtctc atacaaggtt tgcgccttca gccgattgac
     3721 cgcttcaatt tttttcacac cgccctgcgc ctgcagccag cggaacacca gccccgacat
     3781 ataaatcgcg taagttgacg gcgtgttgta cataccttcg cggttcaaat gcgaacggta
     3841 gttgaacaca tcgggaatat cgttcggaca acgctcgagc aaatcctcac gcacaatcac
     3901 caccgtaacc cctgccggcc cgatgttttt ctgtgcgcct gcgtaaatca gtccgtagtc
     3961 ggcaacatca aactcgcgcg acaaaatctc gctggacata tcgcacacca gcggcggcat
     4021 gccttctgaa aggcgcggca cttcacggta ttgcagcccg ttgaccgttt cattgacggc
     4081 aaaatggaca aacgccgaat cgggtgcaac atcccacgtt tccacaggcg gcaggtcgag
     4141 atagtcgaac tgctcgccgc catgcgccgc caaacggatt tccgtatcgg tcaaacggct
     4201 catctgttca taagcgatac ggctccagtt gcccgttacc accgcgtcgg cagtgcggaa
     4261 accgtgtgcc agattcatgg ctgccatatt aaattgggtt gttgctccgc cctgcagaaa
     4321 caatatctta tagttgtcag gcactttcaa aagctgcctc aaatcctgtt ccgcatgatg
     4381 caggatgctc aaaaacattt ccgaacggtg gctcatcgcc atcacgggaa aacccgtacc
     4441 gttgtagtcc aacatttcct gccgcgccgt ttccaacacg gcttcgggca atacggcagg
     4501 gccggcggaa aaattgtaaa tcggataaag agacatgatg cagccttgat tctgaacaat
     4561 aacccgcccg attttaggct tgcagcaggc tttgtgcaag gatggaaaat acctgtcctc
     4621 cgccccgctt catgccgccc gaacacagaa aacaacatca atatattgat ttacaaacat
     4681 aaaaatcatg cacgcgacaa atagatacat ttgttttgtc aacaatattc acgatttccc
     4741 attacaaacc tcccttacat ccgctttttt ccgtcccaaa aacacaaaat aaatcaacac
     4801 tttcatttct ccgcaaaagc ggttataatt acgccgattt ttcaactttg acgaaaaatg
     4861 gccacggggc cattttttta ttatgtacat agggagcagc atggatatcc aaaccatcct
     4921 cgaaaaaacc ctgcccggcc tgggctacga actggtcgat ttcgaactga ccgcgcaagg
     4981 aacattgcgc gtgttcatcg acaaagaagg cggcattacc gtcgaagact gcgcaaccgt
     5041 cagcaaccat ttgagccgcg tcttcatggt tgaagacatc gactacaaaa acctggaaat
     5101 ttccagcccc ggactcgacc gtcccttgaa aaaagccgcc gacttcgtgc gctttgccgg
     5161 tcagaatgcc aaaatcaaaa cccgcctgcc gatagacggt cagaaaaact ttatcggtaa
     5221 aatcgaaggc tgcgaaaatg ataccgttac catatccttc gacggcaaaa ccgtacaaat
     5281 cgaattgggc aacatcgaca aagcccgtct gcgccccgaa ttcaaattct aaaacacagc
     5341 aatattggag atgttcaaaa tgagtcgtga aatgttacag ctggcagaag cactggcaag
     5401 cgaaaaaaac gttgatgcgg aagtcgtctt ccaagcactg gaattcgccc tgtctaccgc
     5461 cgccaagaaa aaggcagacc gcgaacacat ggacgtgcgc gtccaaatca accgcgacac
     5521 cggcgaatac caaaccttcc gccgctggct gattgtcgcc gatgaagact atacctatcc
     5581 cgatgtcgaa aaaaccatcg aggaaatcca agaggaaatt cccggcaccg ccatccaaat
     5641 cggcgaatac tatgaagagc agctacccaa cgaaggcttc ggccgccaag ccgcacaaac
     5701 cgccaaacaa atcatcctgc aacgcatccg cgatgcggag cgcgagcaaa atctgaacga
     5761 gtttctcgcc gtcaaagaag acatcgtgtc cggtacggtc aaacgcgtcg aacgccacgg
     5821 catcatcgtc gaagtcgttg ccggcaaact ggacgcgctg attccgcgcg accaaatgat
     5881 tccgcgcgaa aacttccgca gcggcgaccg catccgcgcc ctcttcctgc gcgtcgaaga
     5941 aatcggcaac atcggccgca aacaagtcat cctgagccgt acttccggcg atttcctcgt
     6001 caaactgtac gccaatgaag tacctgaaat tgcagacggc atgcttgaaa tccgcgctgt
     6061 cgcccgcgac ccgggacaac gtgccaaagt cgccgtcaaa gccaacgacc agcgcatcga
     6121 tccgcaaggc acctgtatcg gcgttcgcgg ttcgcgcgtc aacgcggtca gcaacgaatt
     6181 gtccggcgaa cgcatcgacg ttgtcctttg gtcgcccgag cctgcccaat tcgtcatgag
     6241 cgcgctctca cccgccgagg tcagccgcat cgtcatcgac gaagacaaac acgccgtcga
     6301 tgtcatcgtt gccgaagacc agctcgcgct cgccatcgga cgcggcggtc aaaacgtgcg
     6361 ccttgcttcc gacctgactg gctggcagct caacatcatg acctctgcag aagcagacga
     6421 gcgcaatgcg gcagaagatg ccgccatccg ccgcctgttt atggattact tgaacgtgga
     6481 cgaagaaacc gccgacgtac tggttcagga aggttttgca accttggaag aagtcgccta
     6541 tgttcctgcc gccgaactgc ttgccattga aggatttgac gaagaaatcg tcgatatgct
     6601 ccgcaaccgc gcccgcgatg ccatcctgac catggcgatt gccgccgaag aaaaactggg
     6661 cgaagtatcc gacgatatgc gcaacctcga aggcatagat gctgatatgc tccgcagcct
     6721 tgccgaagca ggcattacta cccgcgacga cttggcagag cttgccgtgg acgaactgat
     6781 tgaaatcacc ggtgtaaacg aagaaaccgc aaaagccgtc atcctgaccg ctcgcgaaca
     6841 ctggtttacc gaagacaaat aaaagggggt acagatgagt aacacaaccg tagaacaatt
     6901 tgccgccgag ctgaaacgcc ccgtcgaaga cctgttgaaa cagttgaaag aagccggcgt
     6961 cagcaaaaac agcggcagcg attccctgac gctggacgac aaacagcttc tgaacgccta
     7021 cctgaccaag aaaaacggca gcaacggcgg caccatcagc atccgccgca ccaaaaccga
     7081 agtcagcacc gttgacggcg taaaagtcga aacacgcaaa cgcggacgca ctgtcaacat
     7141 tccttctgcc gaagagttgg cagcacaggt aaaagccgcc caaacccaag ccgcacctgt
     7201 ccggccggag cagacggcag aagacgcggc aaaagcccga gccgaagctg ccacacgtgc
     7261 agaagcccgt gccaaggcag aagcggaagc ggcaaaactg aaagcggcaa aagcaggcaa
     7321 caaagccaaa cctgccgcgc agaaacccac cgaagcaaaa gccgaaaccg cacccgttgc
     7381 ggcggaaacc aaacccgccg aagaaagcaa agcggaaaaa gcccaagccg acaaaatgcc
     7441 gtctaaaaaa cccgccgagc ccaaagaaaa agccgccaag ccgaaacacg agcgaaacgg
     7501 caaaggcaaa gatgccaaaa aaccggcgaa acctgccgca cctgccgtgc cgcaacccgt
     7561 ggtcagcgca gaagaacagg cgcaacgcga tgaagaagca cgccgtgccg ccgcacttcg
     7621 cgcccaccag gaagccctgt tgaaagagaa acaggaacgc caggcacgcc gcgaagccat
     7681 gaaacaacag gcagaacaac aggcaaaagc cgcacaggaa gccaaaaccg gcagacagcg
     7741 tcccgccaaa cctgccgaga aaccgccggc agccgcgccg gccgtcgaaa acaaacctgt
     7801 caatccggca aaagcgaaaa aagaagaccg ccgcaaccgc gatgacgaag gtcaaggccg
     7861 caacgccaaa ggcaaaggcg gaaaaggcgg acgcgaccgc aacaatgcac gcaatggcga
     7921 cgacgagcgc gtacgcggcg gtaaaaaagg caaaaaactc aaactcgagc cgaaccaaca
     7981 cgccttccaa gcaccgaccg aacctgtcgt acacgaagtt ttggtgcctg aaaccattac
     8041 cgttgccgac ttggcacaca aaatggcagt caaaggcgtg gaagtggtca aagccctgat
     8101 gaagatgggc atgatggtta ccatcaacca atccatcgac caagacaccg ccctgattgt
     8161 cgtcgaagaa ctcggacaca tcggcaaacc cgccgccgcc gacgatcccg aagccttctt
     8221 ggacgagggc gcggaagcgg tggaagccga agcattgccg cgtccgcccg tcgttaccgt
     8281 catgggccac gtcgaccacg gcaaaacctc gctgctggac tacatccgcc gtaccaaagt
     8341 ggtacagggc gaagcaggcg gcattaccca acacatcggt gcataccacg ttgaaacccc
     8401 acgcggcgtg attaccttct tggatactcc gggtcacgaa gcgtttactg ccatgcgtgc
     8461 gcgcggtgcg aaagcaaccg atatcgtgat tcttgtggtc gccgccgacg acggtgtgat
     8521 gccgcaaacc atcgaagcga ttgcccacgc caaagcggca ggcgtaccga tggtggttgc
     8581 cgtcaacaaa atcgataaag aagccgccaa cccagagcgt atccgccaag agctgaccgc
     8641 acacgaagtc gtgcctgacg aatggggcgg cgatgtacag tttatcgacg tttccgctaa
     8701 aaaaggcctg cacatcgatg cattgctcga ggcagtcttg ctcgaagccg aagttttgga
     8761 actgaccgca cctgtcgatg cgcccgccaa aggcatcatc gtcgaggcgc gcttggacaa
     8821 aggccgcggc gcggttgcca cattgctggt tcaaagcggt acgctgaaaa aaggcgacat
     8881 gctgctggcc ggtacggcat tcggcaaaat ccgcgcgatg gtcgatgaaa acggcaaatc
     8941 cattaccgaa gccggcccgt ccatccccgt cgaaatcctc ggcttgtccg acgtaccgaa
     9001 tgcgggtgaa gacgcgatgg tattggcgga cgagaaaaaa gcgcgcgaaa tcgccctctt
     9061 ccgccaaggc aaataccgcg acgtgcgcct tgccaaacag caggcggcga agctggaaaa
     9121 tatgttcaac aatatgggcg aaacccaggc ccaatctttg tcggtcatca tcaaggcaga
     9181 cgttcagggt tcttacgagg ctttggcggg cagcctgaaa aaactgtcca ccgacgaggt
     9241 gaaagtgaac gtattacaca gcggcgtggg cggcattacc gaatcggatg tcaaccttgc
     9301 catcgcgtcc ggggcgttca ttatcggctt taacgtgcgt gcagatgcct cttcgcgcaa
     9361 acttgccgaa aatgaaaatg tggaaatccg ctactacaac atcatctatg atgccatcga
     9421 cgacgtgaaa gccgccatga gcggtatgct ttccccggaa gagaaagaac aggttaccgg
     9481 tacggtcgaa atccgtcagg tcatctccgt ttccaaagtc ggcaatattg caggctgtat
     9541 ggttaccgat ggtgtggtca aacgcgattc ccatgtccgc ctcatccgca acaacgtggt
     9601 tatccatacc ggcgagctgg cttcgttgaa acgctataaa gacgacgtaa aagaagtccg
     9661 catgggcttt gagtgcggtc tgatgctcaa aggctacaac gaaatcatgg aaggcgacca
     9721 actggaatgc ttcgacatcg tcgaagttgc ccgcagcctg taattccttt gcaaataaaa
     9781 tgccgtctga agcgttcaga cggcaacgaa acgggttctg tatcatacag aacccgtttt
     9841 ttgtcgcaaa tcggcttcag acagccctct tgccttatcc cgatttgaat ctgacttgcc
     9901 atacaaacag gcttcagacg gca
//
LOCUS       DAXHKX010000058         9795 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHKX010000058 DAXHKX010000000
VERSION     DAXHKX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.058"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(9..1304)
                     /locus_tag="ACFMWZ_001105"
     CDS             complement(9..1304)
                     /locus_tag="ACFMWZ_001105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC5935076.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1430..2317)
                     /locus_tag="ACFMWZ_001106"
     CDS             complement(1430..2317)
                     /locus_tag="ACFMWZ_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC5935077.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2317..3282)
                     /locus_tag="ACFMWZ_001107"
     CDS             complement(2317..3282)
                     /locus_tag="ACFMWZ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC5935078.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3298..4422)
                     /gene="potA"
                     /locus_tag="ACFMWZ_001108"
     CDS             complement(3298..4422)
                     /gene="potA"
                     /locus_tag="ACFMWZ_001108"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC5935079.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4617..4724)
                     /locus_tag="ACFMWZ_001109"
                     /pseudo
     CDS             complement(4617..4724)
                     /locus_tag="ACFMWZ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="ACFMWZ_001110"
     CDS             complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="ACFMWZ_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC5935080.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5295..6230)
                     /gene="secF"
                     /locus_tag="ACFMWZ_001111"
     CDS             complement(5295..6230)
                     /gene="secF"
                     /locus_tag="ACFMWZ_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC5935081.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6234..8087)
                     /gene="secD"
                     /locus_tag="ACFMWZ_001112"
     CDS             complement(6234..8087)
                     /gene="secD"
                     /locus_tag="ACFMWZ_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC5935082.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPEILLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEIAGGKE"
     gene            complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="ACFMWZ_001113"
     CDS             complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="ACFMWZ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC5935083.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8693..>9795
                     /locus_tag="ACFMWZ_001114"
     CDS             8693..>9795
                     /locus_tag="ACFMWZ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC5935084.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2504 a         2588 c         2443 g         2260 t
ORIGIN      
        1 tgcgacgctt aacggctgtc tttccactga taatatttcg agccgaggaa agaaccgagt
       61 ttgcgcaaaa acggttgcag gataatattc ggctggcgca accgctcaaa cggcttcaga
      121 cggcattcgt cccctaaaat tgcttcggca accgccagac ctgcaatgcc tgttatcgcc
      181 atcccgtgtc cggaataacc ttgcgtataa aaaacattcg gggctaaacg tccgaaatgc
      241 gggacaaggt tggcggtaat gtcgcactcc ccgccccacg aatattcgat tttgacatcg
      301 gcaagctgcg gaaaaacttt aagcatatct tggcggacaa gctcggtcat acgcgcggga
      361 ttgtcgataa actcgttatc cttaccgccg aaaagcagtc tgccgtccgc gctgaggcgg
      421 taataatcca aaatatggcg gttgtcgcat actgccatat tgttgcggat aagccctttt
      481 gcacgcgcgc ccaagggttc ggtggcaata ataaaggtgc tgacagcaat cgccttgcgc
      541 tccaaaggct tgaatatcgg attcaaaccg gcataagtat tgacggcgta caccagattt
      601 ttgcattcga cgctgccttc gggggtgtaa accagccaac cgttttgatg cggttcgatg
      661 cgcgtcatcg gggactgctc gaaaatctgt gcgccggctt cggcagcggc gcgggcgatg
      721 cccaacgtgt aattgagcgg atggagatgc ccggacaagg gatcgaactg tgcgccttgg
      781 tacatatcgc tgtcaagctg ctgcttcagt tcagtgttat cccagagttg ataatgactc
      841 gcaccgtaat gccgttgggc gtgttcatgc cactgctgca actcttccca atgctgcgga
      901 cggacggcaa ccgtggcata accgcgctgc caatcgcaat caatggcatg tttgcggacg
      961 cgttcgtcca ccagttcgac cgcctgcaaa gactgttgcc aaaaccattg cgcctgctcc
     1021 aagccgacct gtttttcaat ttcccccata ccgcaggcgt aatcgctgat aacctgcccg
     1081 ccactccgtc ccgacgcgcc gaaaccgata cgcgcggctt ccaacacaac cgtttcatgt
     1141 ccctgctccg ccaagggcaa tgcagtgcac aaaccactca atccgccgcc gatgatacag
     1201 gtatcggttt tcagacggca ttgaagtttc ggataaacag tatgaggatt aaccgaactg
     1261 aaataataag aaggcagata ttcttgaaaa tcagggcgaa tcattgtgtt tgctttatca
     1321 ggtgtatttt cggacggaat gatacaggct gtcgggccat atcgtccaaa cagaaaatcg
     1381 gttgaagaaa acaggctgac ccagtcatgc ggtcagcctg ccttattaat taatttgctt
     1441 tctcggcagc caatttttct tggcggtagg cttctgccgc ttctcggtca cgcttggttg
     1501 cctgcctcat catccaataa ttgacgatga tgaccaatgt tccgatgatg ccgattagga
     1561 tggtcgccaa gacattcatc tgaggatcaa gacccagctt gattttggag aaaatcacct
     1621 gcggcaatgt ggatgaaccg gggccggaga ggaatgaggt aattaccaaa tcatccaaag
     1681 acagggtaat gccgagcaga aagcctgaag cgatggcagg ggcaatcaaa ggcaaagtga
     1741 tgacaaaaaa gattttcagc ggacgcgcgc ccaaatccat tgcggcttct tcgagcgatt
     1801 ggtcaagctc aaccagacgc gaacggataa caacggtaat gtacgccata cacagcgtcg
     1861 tatgtccgag gaagatggtg aaaaagccac gatcgaagta gagatgttgt aaccattcgc
     1921 tgccctgcaa aaatatctgt acctgaataa tcagcagcag catagacaga ccggtaatca
     1981 cgtcgggcat caccataggt gcggaaatca taccggcgaa caaggtactg ccgcgaaaac
     2041 gtttaatccg cgccatcgca tagcctgcca gcgtgcccaa aacgacggcg gcaagcgaag
     2101 acacaacggc aatccgcagc gacagccaag cggcttccaa gatggtgtcg ttttccagca
     2161 atgcgccgta ccacttggtc gaaaagccgc cccaaacggt taccagcttg gattcgttaa
     2221 acgaatagat gaccaaaaca accagcggga tatacagaaa cgccagcgac agtgccaaca
     2281 tcagtttcaa gaaccaagat aatttggatt tctgcattat ttggctcctt cttccaattc
     2341 gcggttttca taatgctgaa acagggcaat cggcacgacc agcagcgcga ccatcacgac
     2401 ggcgacggcg gaagccagcg gccagttgtt ttgatcgaag aacgcctgcc acaagacttt
     2461 accaatcatc aggttttccg aaccgccgac cagctcggga atgacgaact cgccgacagc
     2521 agggacgaaa accagcatgg agcctgcaat aatgccggtt ttcgacaaag gcagggtaat
     2581 cgtcaagaac gatttgaccg gccccgcgcc caaatcggaa gccgcttcaa gcaggcggtt
     2641 gtcgagtttc accagttgcg tgtatagcgg cagaatcata aacggcagat aggcgtaaac
     2701 catcaccaaa ttgagcgaaa aggcattgta gaacaaatcc aaaggctcgc tgataatacc
     2761 cattttaatc aacaggttgt ttacaatacc gttatgcccg agcagaccca tccacgcata
     2821 gacgcgcaac aggaacgatg tccaaaaggg cagcataatg gcaagcagca aaccattgcg
     2881 gacagaagga ttggcacgag aaatcgcata ggcggtcgga taaccgacca acaggcaaat
     2941 cactgtcgtc atcaatgcgg ttttgattga agaccagtag gtcatcagat agatattgct
     3001 gttttcactg tcgccgaacg ggttaagggt atgccaaaaa ttttggaaaa tatcggcata
     3061 gttttgataa ctgatggtga tattcagacg acctaaatct tcgtcgatgc tggtcagcgg
     3121 cgtaaacggc ggaatggcga tttcttgttc ggcaaagctg attttcagca cgatggcgaa
     3181 cggaatcaga aacagcacca aaagccaaat atacggtacg gcaatcacca cacgctgtcc
     3241 cgggcggcgg aacagtttgt ttttcagttt attaaggttc attgcattcc ccttaaatca
     3301 acggaacagt ggcgtaggct ggttttccgg ccagctgata tacacggttt cgtcccaagt
     3361 tggcggtgta atgttgcgta cataccagta aggtgcagga acttggcttt tgacgacgcg
     3421 cccgttggcg agtttgatat ggtatatggc aaagctgccc aaataggcga tttcttttac
     3481 cgtgcctttc gcccagttgt agtcgcccaa atattcgggt ttttctttat ataaatcaat
     3541 atcctctggt cgaatactaa cccaaaggtc ctgctcgctc ggaccaccca aaccgtgatc
     3601 gatgcggacg tggttttcca aaccttcgca ttcgataacg gcatagtcgg catgatcttc
     3661 aatcaccaca ccgtcaaaga tgttggtttc gccgataaac tcggcagtga agcggctgtt
     3721 gggatagtcg tacacgtcgc tgggtgtgcc gacttgctgc aactgaccgt cagacataat
     3781 ggcgatgcgg gtcgccatcg tcatcgcctc ttcttggtcg tgcgtaacca taatacaggt
     3841 tacgccgact tgttccagcg tattgaccaa ctcaagctgg gtttgttggc gcagtttttt
     3901 gtccaatgca ccgaggggct catccagcag tagaattttc ggacgttttg ccagactgcg
     3961 tgccaaagca atgcgctgct gctgaccgcc ggacaattgg tgcggtttgc gtttagcaaa
     4021 tttggtcatc tgaaccaggc ggagcatttc ttcgacgcgc gcggcgattt cgcctttagg
     4081 cattttgtcc tgtttcagac cgaaggcaat gttttgttct acggtcatat gcggaaaaag
     4141 cgcgtaactt tggaacatca tattgatggg gcgatcatag ggtgcaagtt tggtaatatc
     4201 ctgaccatca aggataattt ttccctgatt gggactttcc atacccgcca gcatacgcag
     4261 cagtgtagat tttccgctgc cggaactgcc caaaagggcg aagatttcgt gttgataaat
     4321 gtccaagtcg atgttatcga cagcgtaatt gtcaccaaac tttttcacca aaccttggat
     4381 tttgagataa ggtttggctg aagacgcagt ggttgcggtc ataatggcaa tactccaata
     4441 aaaagacgag taccggcaaa acggattttc gaatgggtga taaaaagctg tttgattgct
     4501 ggcgggagtt aaacgtttga tgccgtctga aactcttgta aagcgcacgg gcagcatgaa
     4561 atggaacaag attccaaaga actttatatt atattagttt atgcggtttt cgggcaatat
     4621 agtggattaa atttaaacca gtacagcgtt gccttgcctt gccgtactat ttgtactgtc
     4681 tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac tataaatatg gatttgagct
     4741 tgtcggaaag caacagaaaa gaaaacaccg cccatttttc tgggcggtgt cggaaagcgt
     4801 aattatttac gcagacccaa gcgggtaatc aacgcgcgat acgtatcggg ctgggtacgg
     4861 cgcaagtagg ccagcaggcg gcggcgttgg ctgaccattt tcaacaggcc gcgacggctg
     4921 tggtggtctt tggggttggc tttgaagtgg ggggtcaggt cgttgatgcg gaaagtcaac
     4981 agagcgactt gtacttcgga agagccggtg tcgccttctt tgcgttggaa atctttaacg
     5041 atttgtgctt tttgttctac ggtcagtgcc ataatgaaaa ctccaaaaat ataagaatcc
     5101 ccatagggat tcagacaagt ttgccaagcc tgaagacaac ggcaaactcc ctatgcccaa
     5161 gataggacaa cgtggcatta tgacacaatt cccgcatttc tgcacaatat tttaagaccg
     5221 tggaagtaac cgttaaaaat gccgtctgaa gcattgtctg cttgagacgg caatgttcag
     5281 acggcatatg cgcttcaaac cacgatttct tctttcttct tcggttcttt accgatattg
     5341 tcgcggctca aaccgaacat tagcagaagc gggctggcaa ccaatacgga agaataaatg
     5401 ccgaacacga tgccaatggt caacgccata gaaaagccgt gcaaggccgc accgccgaac
     5461 accagcatag aaacaaccat cgcctcggtc gaaccgtggg taatgatggt gcggctcatc
     5521 gttgcggtaa tcgcgttgtc gatgacttcc ggcacggcat gtccgcgcat cgccggcttg
     5581 cggaagtttt cacggatacg gtcgaagacg acgacggatt cgttcacaga atagcccaat
     5641 acggcaagga tacccgccaa gacggtcagc gaaaattccc attggaagaa ggcaaagcag
     5701 ccgagaataa tcacgatgtc gtgcatattg gcgataatgg cagatacggc aaaacgccat
     5761 tcaaaacgca tcgacaggta aataatgatg ccgataacga caaaacctaa agccatcaat
     5821 ccattactta ccaattcctc accgacttgc gggccgataa attcgacttg gcgcaaggta
     5881 acgtcgggac tgtctttttt cagcaaatcc ataacctgat tggacaactg tgcggaagta
     5941 acaccttctt tgttcggcag gcggatcatg atgtgtttgt tcgtacccaa tgcctgaacc
     6001 tgtacatcac ctattttcag cgtatcgagg cgttcgcgca tcttattgac atccgcaccc
     6061 tgctgatatt ggacttccat taccgtaccg ccggtaaatt cgacagagaa attcagacct
     6121 ctggtaacca aaaagaatac ggcggcaata aaggtaacca acgaaataaa ggtcgtcagt
     6181 ttgccgtagc tcataaacgg aatatcgcgt ttgattttaa agagttccat agcttactcc
     6241 ttgcctcctg ctatttcggc tttcggcttc cacaccgaac caatggaaat attctgcaat
     6301 ttgcgtctgc gtccgtacca cagattgacc aacgcacgga atacgacgac ggacgaatac
     6361 atcgaagtca gaatacccaa acagtgtacg accgcaaaac cgcgtaccgg gccggaaccg
     6421 aataccaaaa gcgcgatacc ggcaatcagc gaagtcaggt tggaatcgac aatggtcgcc
     6481 catgcgtgtt ggaaaccgag attgattgcc tgctgcggcg gcacgccggc acgcaattct
     6541 tcgcggatac gttcgttaat caagacgttg gagtcgattg ccatacccaa agtcaacgcc
     6601 agcgcggcca tacccggtaa cgtcaacgtt gcctgcatag cagacaaaat accgattagg
     6661 aacagtatgt tggcactcaa tgcaatggta gaaaagaaac ccatcagacg atagtaaacc
     6721 accatgaatg cagcaacgat ggcaaaaccc cataaagtcg aatggaagcc tttttcgatg
     6781 ttctccttac ccaaagacgg accgatggta cgttcttcga caatctgcat cggtgcggca
     6841 agagaaccgg cacgcaacag caaagacgta tcattggctt cggctgtcgt catgcttccg
     6901 gaaatttcca cgcgtccgcc tgtaatggca gtacggataa ccggcgcggt tacaacctcg
     6961 gattttcctt ggtcgatcaa aaccatcgcc atgcgtttgc cgacatttgc ggcagtcagt
     7021 tcgccgaaaa tgctgccacc cgcgctgtcc aagctcagac tgacggcagg tgcgcccatt
     7081 tggtcgaaac tcggttgcgc atcgttgatg ttgtcgcccg tcagctcgac ctgtttgctg
     7141 atcagcagaa tttcgggacg atctccgccg cttgaaagca gctcataacc gctcggcacg
     7201 ttgccttcca atgcctcgcg caacttggca ggatcgtcct ccaccatacg caattccaaa
     7261 gtcgcggtac ggccgatgat gtcttttgcc ttggcagtat cctgaacgcc cggaagctgc
     7321 acgacgatac ggtcggcacc ggactgctgg atgacgggtt cggccacgcc caactcgttc
     7381 acacggttgt gcagggtagt gatgttctgt ttgaccgcat cggaacgcac tttattgacc
     7441 gcctcttccg aaagcgtcaa gacgatattg ctgccgtctg aattcagcgt tgcttcagga
     7501 aacagcttgc gcaactgcgg cagagccttt tgcacatcac ctgcatcctg caaagggacg
     7561 gtcaggctgt ttccagcctg acgcaccgtg ccgctgcgga ttttttcgcg gcgcagttcg
     7621 cggcggatgt cgcccgaata acgttcaaac gttttctgca tcgccgcttt catatcgacc
     7681 tgcatggtga aatgcacgcc gccgcgcaag tccaaaccca aaaacatcgg attggctttg
     7741 attttcgcca tccattcggg gctgtccgcc aacaggttga gcgcggtaat atagccttcg
     7801 cccaaagtgt tttcgatgac gtcgcgcgct ttaagctgcg tttcggtgtc tttgaaacgc
     7861 actttcagtg aattgtccac aacaaacatc ccgtcggtct gaatacccgc gttttgcagc
     7921 gcggcatcca ctttggattg agtctgttcg ttgatgatga tggcttgtcg gttggtcgat
     7981 acctgcacgg cgggtgtttc gccgaatagg ttgggcagcg aatacactgc ggcaaccgca
     8041 atcgtgaaca caatcagcag atatttccat aaaggataac ggttcatcat tgttccttaa
     8101 tggttggaac cccacccttt cggtggtgtc ggaatcgggc tatttcagaa gaggcaaaaa
     8161 cccttcccag ccaggcaaga ccggaaggcg gcatcttgaa tatgccgccc tgcgtgtcgg
     8221 aacatggtca agccttcggt tggaattcaa aacaaagtgc cgcattcggg ctttccagat
     8281 gcggcttgtc ggcacaaatc aatcgacttt tgcggcaatc gcattgcgtt ccacttcgac
     8341 ctcgattttt gtaccctgtc cgatatccac ggtaaaaaac tgttcgccga ctctggttac
     8401 cttacccttg aaacctgccg ccaagaccac tttgtcgccg actttcaagg cggcaagcat
     8461 tgcctgatgc gctttgaatt tcttttgctg cggacgcatg atcaggaagt agaacaccac
     8521 cataatcaac actaaaggag caaattgtgc aacagcttga ttcataattt atccgttctt
     8581 tctaatatgg ttgaaaatcg agaggggtat ataataacat aagaccgtaa acaatatatc
     8641 gggtttgccg tccgtaccga ccgtataccg cagcctgccc gtccacaaac ccatgtcctt
     8701 gacccgccta atcttgaaat tctatgcact gttgcgcctt tttttgggca aaaacgcccg
     8761 caccgcatgg atttcgcatc ccgcctgtgc cgggcacgaa cccggcgcaa accatcccga
     8821 ttcgcccgac cgcatcctct gcatcgagca ggcattgcgc cgcgccggta tttggcagca
     8881 cctccaaacc atagaggcgg aagaaatcag cgatacgcgc ctcgcacttg tccactcgag
     8941 caaatatctg aaccgtttgg aatcttgcct gccccaaaaa ggcaagattt cccgcctgga
     9001 taacgacact gcaatcagca caggctcgct gtctgccgca cgctttgccg ccggttcggc
     9061 agttcaggca gtcgacatgg tcatgaaccg taaagcatgg catgcctttt gcgccgcccg
     9121 cccgcccgga caccatgcgg gcagcggcaa agccggcgga ttctgcctgc tgaacaacgt
     9181 tgccgccggc gtcatgcatg ccattgccga ataccgcctg aaacgcattg ccgtcatcga
     9241 tttcgatgtc cactacggcg acggtacggc agaaatattc aaagacgatc cgcgcatcct
     9301 gtttttcaac ctgtttgaaa ccgacctttt ccccttcccc gaaaacaacg atatgcccga
     9361 cggcggcaat atggtgcacc tgcccttgcc gccgggaacg ggcagccgca cattccgcga
     9421 agccgtccgc aggcagtggc tgccccgact tgccgcattc aaacccgaac tggtgctgct
     9481 gtcggcagga ttcgacgcac accgtctaga cgaatcgggc aggctcaacc tgcacgaggc
     9541 ggattttgcc tggctgacac acaaaatcat tcagacggca tcgggctgcc ccggcaaaat
     9601 cgtatccgta ctcgaaggcg gctacaccct tgaacctctg gcacaatctg ctgccgagca
     9661 tatccgcgtg ctggcagggc tgggcaaatc cgatgccgca accgcctatc agaaaacact
     9721 ggacccaaca aaaaaacggt ttgccaaacc gaaaaccgga caggtgcgcc aacctaccca
     9781 atccgaccgt tacga
//
LOCUS       DAXHKX010000059         9756 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHKX010000059 DAXHKX010000000
VERSION     DAXHKX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.059"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(121..4290)
                     /locus_tag="ACFMWZ_001115"
     CDS             complement(121..4290)
                     /locus_tag="ACFMWZ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC5935085.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRLLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     HFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNVAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNASLSAAAGALLAGQINDRIGLVDDLGFTRKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
     gene            complement(4345..6192)
                     /locus_tag="ACFMWZ_001116"
     CDS             complement(4345..6192)
                     /locus_tag="ACFMWZ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC5935086.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFNSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6606..7835
                     /gene="sstT"
                     /locus_tag="ACFMWZ_001117"
     CDS             6606..7835
                     /gene="sstT"
                     /locus_tag="ACFMWZ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC5935087.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIILYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            7947..9230
                     /locus_tag="ACFMWZ_001118"
     CDS             7947..9230
                     /locus_tag="ACFMWZ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC5935088.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVVE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHTENGRPY
                     PTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGR
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9306..9521)
                     /locus_tag="ACFMWZ_001119"
     CDS             complement(9306..9521)
                     /locus_tag="ACFMWZ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935089.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9514..9729)
                     /locus_tag="ACFMWZ_001120"
     CDS             complement(9514..9729)
                     /locus_tag="ACFMWZ_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC5935090.1"
                     /translation="MKEYKVVIYQESQLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT         1937 a         2501 c         3028 g         2290 t
ORIGIN      
        1 tcctgatttt tgttaattca ctatatcgat tctgttccct tgcaggcgga cggcggcggc
       61 ggacggtgga aaagcaggtt ttcaaatgtc cggtttggcg gcgcgccgtc tgaaaaccgc
      121 ttattttcct ttgccgtttc cggcggagtc ttttttgtcc gaaccggaga agcggtcgaa
      181 acgtatggtg tatgtcagct cgccgcccga cgaacggctg ccgatacggg caaccgcctg
      241 tatggcgcgg gtcagccggt aaatcagttt gacggactgt tccgcgctgg agatgctgta
      301 ttcgtagccg atgtagagtt tgccggtcag ttgtttgccg acggtcagca cctgttcggc
      361 ggggttgagt tcgccggttt gcgcgttgcg gctgcgcttg cgggtaaagc ccaaatcatc
      421 caccagcccg atgcggtcgt tgatttgccc ggcaagcagc gcgcctgcgg ctgcggacag
      481 ggaggcattg tcgccgctgc tgccgctgcc ggcgcggttg aggatgagcc aagagagctt
      541 gtctttttca ctcatcggtt cgtttgccgt cagcgtaatg cgcgggctgt tgaggctgcc
      601 caatatttcc acgcccgcac cgacggggga aaggcggcgt tcggcgcgga tgttgaggtt
      661 ggggtcgttg agcgggccga caaaggagac cgtgcctttg gtaatgtcca aatcctgccc
      721 gtatgcctta taacgccctt tgatgacgcg gaccgtgccc acgccccgca cgcttccgcc
      781 cgattgggcg gtcagggtca gtttgccgcc tatggtaacg tccgcgccgt agccggcgaa
      841 gcggatgccg tcattgaggt ctaaagtcag gttcatattg acggggagcg gtgccgccgc
      901 ctcttttttg acttcgccta atacgacgac atcgtcgccg acggacggca tcgaggattt
      961 ttgcgaaccg aacagcccct gatccgtttt aatcatcccg gtaacggata tgcctttttg
     1021 cggcgaatag cgcaggcggg tgttgccgga aaccgtcagg cggcggttgg ggcgggacag
     1081 gatgcggtat ttgtcgaaca ccgcgccgat atcgacatcg ggtccgctgt tttccatacc
     1141 gaccgtaccg gagagttccg ccgtcccttc gtgacggaat ttcaggctgt cgattaccca
     1201 tttcctgcct gcgatatgcg aacgcagcga accgttgtcc aagatgatgc cttgggtttg
     1261 gttgcggtaa tagagcttgt cgccgttgat gctgccgccc aagtgcgggt cggcgatgct
     1321 gccgccgagg gttacggcgg cattcaggct gcctttgacg gtttgtccga cgggcaggaa
     1381 gttgcggaat acttcggcat cggcaacggt caggttgagc ctgccgccca aaggcgcggt
     1441 atcgaaagag cggctttgcc cgacggtgat gttgccgttg attttcccgt agttgctgct
     1501 gccgttgacg gcgacattga cggacggaga gccgacccgt ccgccgattt gcgcggcggc
     1561 attcaggctg ccggtaatgt tttgcgcggc ggcgggcaga aagggcttca atgcgcccaa
     1621 gtcgggaagg gaggcggtaa tcctgccgcc gagcggtgca tttgccatat tgccgccgaa
     1681 ggcgttgccg atgtccaaat cggcgttaat ccgcccgaaa tgcgcgccgc cgtcaagcag
     1741 gattccgatg cggtcgtttt gaaagcgcgt tttcagggaa aatgcgttca aacccaaagc
     1801 ctgcccgccg ggcaatacgg catcgccgct ttgccggctg atattgaggt agccgcgcgc
     1861 gttgcgcccg taggcgacat cccagtcgcc gtttaaaacc agattgtgtt cgaagggcgg
     1921 tttgaagaaa ttgtgcaact cggcgatatg cagaccgcgt gcgccgcctt ttgccgatat
     1981 gccggttttc ctgtcccagg aaaagtgttg caggttgagg ctgccgccca ttgcctgcca
     2041 atttgccgca cttgccgcca cgcgttccgc accggcttcg agcgtcatac ggttttgcag
     2101 cttgaggttg aatgcgccgc cgatgtcgag gatgccgatg ctgcccttcc atcgggtaag
     2161 ttccctgttg atgccgcctg aagcgtccaa atcgaatttg aacggtttgc catccagcgt
     2221 catggcggcg tgtgtgcgga tgcggtgctg cacgcccgtg ccgtccagca tcaggtcggc
     2281 ggtatcgaca accgccgctc cgcccgacag cgaaaggcgg ctgcctttga tgtcggcgcg
     2341 tatcgggcgg cttgtgtcgg gcgaaccttt gagggtgaaa tcgagcgaac ggatgtctgc
     2401 cgccttgccg atgtgcaggt tgcgcgccgc gccggaaagg tcggtttcaa aggttcggat
     2461 gccaccgtcc aaatcgccgg aaaggtgtcc gcgtacattt aaagaccccg cgagtccgaa
     2521 accgaaacgg gataaatcgg gtgcggtgat attgaggtta agccggtcgc cttttttgcc
     2581 gaagccgccg tctgttttaa taatgttccg ccccagccgc aaatcgacgg cggcacgcgg
     2641 aaggtggcgg gactcgtaaa caatgtcggc actgccggca atcggtacgc cgttgaacgt
     2701 gccgggtaaa aaccgcattt tgcctgtgaa tttctctttt gccagttcgc cggcaaggtt
     2761 tattgagccg ttgatattgc ctgccggaag ttgcggatcg atgcgcgaag ggtcgaatgc
     2821 gcgggaacgg atgtccagct tgagcaggcg gtctttaaac agctcgagat agccttgcgc
     2881 ggtcaggctg ccttgcccgg cggcgatgtt gacggtgtcg agcaccagtt tccgctgtcc
     2941 gtttgcgggg tcgcttgcaa tggcgaggct gccgtccgtg cgcgccgtgc cgatgccgag
     3001 ttgccaagag attttgggcg aggcggtcgt gccaccgatg ccgatgctgc cgtccaacct
     3061 gcctttgaac gcggtttgca gtacgtcttc cgcgccgacg gagttgatgc ctatatttaa
     3121 atcgaggatg tctttttcgg tgtcgatttt gcccgacagc ctgatgccgc cccgtccgag
     3181 cagggcggcg gacgtattgc cgatatgcac cgtgccgtcc tgccggatga caaagccgcc
     3241 taaaacctga cggacgggga tgccgttgcg gtcggcaaag ccggctttgg tgttttccaa
     3301 atcgagcgaa ccttccagcg cgatgccgtc tgaaaacgac gggatggcgg tcaggtcgaa
     3361 attcagtccg gcatcgggca gggaaggcac gaaggcggcc ggattgatgt tgaacccttt
     3421 gaccagtact tcttccaatg ttttatccaa tgattcggca aacgggtgga tgacggattt
     3481 tcccgagagg cggatattgc cgccgtcgat cgccagttcg gcgcgcacat ccttcaggct
     3541 gccgctcagc cgagccgtac tgtgtatggt tttgccttcg agtccgcctt tggtgtaaat
     3601 ggcggtatcg agggcaaacg gttttttcaa gccgaccgag gccgcccccg acgaactgct
     3661 ccacggcgtg tcggcggcct tcaggtcaag gcggtgtcct ttgcggtcgt aacggtatga
     3721 agcatccagc cgttcgagat agacggtttg tttgtcaaag gctttgccca tgctgatttt
     3781 gcccgtctcg aagcggtcga gatagacggc ggcaggcagg tctatgctgt cgggaaggct
     3841 gagcggcggg cgttcttctt taggcggagt cggtttggta acgatggcga tgtcgccggc
     3901 ggaaatttcg gtaatgtgca ggctgcggcg catcagttcg gacggtttcc acgcgaagcg
     3961 gaagcggctg attttaaggt ctgccccctc ggtttctatc gaccagttgt cgccgtcgaa
     4021 gccgtcgagc agcgtgcctt tgaggttttg ggaggaaatg tttacgccga accaagacgg
     4081 gatttggtac agcccgaagc gcaaacctgc ttccgtaccg gcgagccagc cgaggaaaca
     4141 tactgccaaa atcaggacag acagcagtgc cgccgacagc ttcagcagca ggcggcgttt
     4201 ttttgccggc ggggtggggc ggtgttcaga cggcattttg cgcgtgccgt tttcggtcgg
     4261 atcggtatct gtcggtgcgg tatcggtcat aatgtctgtt tcaaaatagt ttgctgcaat
     4321 gccgtctgaa gcggccgtat cggtttagaa gcgcgttccc aggctgatgt gccagcggat
     4381 tttcttgtcg ctgtgcccgt aggcgatgtc gaaggaaaac ggcgcgagcg ggctgaacca
     4441 gcgcacgccc agtcccgaac cgtgtttcag cttcatacgt ttgaaattgg cggcggcatc
     4501 gcccatatcg tggaataccg cgccggaaag ggtgcgcgta aacggcagtt ggtattccag
     4561 gctgcccacc aagagggcgc gttcgggcag gaccgatccg ttcgggccgg caagtccgat
     4621 gctgtcgagt tcgtaaccgc gcacggaaga cgcgccaccg ctgcggaaca tcagccccga
     4681 aggaacgtcg gcattgtcgc gggcaacggt gtaacccgct tgtccgcgta tgatgaacgt
     4741 gccgagtttc ttgttttcgg gcgtgaagaa ataacctgcg cgggcagagg tgcggatcag
     4801 cgcggtggag gacaggaatg tgcccaaagt cgtaccgatt ttgccgtcga ggtaatggcc
     4861 gttttcgggg tgcagcacgt tgttgagcag ctggcgtttc caagaggcgg tcagcatcgt
     4921 ggcgtggctg ttgcccaaat cgatatccga gccggggatt ttccggcctt ctgcgagaaa
     4981 ctctgccccc agcctggcat cgatgcccgc gcggtcgcgc acataccaga tgccgccgga
     5041 gaaggcgcgt ttttcgaggt tttgggtggt cgaacggttg taggaaacgt tgcttgtcca
     5101 gtagttgccc cgatagttgc gcggctggct gatgccggcg gcaagcgtgg tttcgtattt
     5161 gtccatatcc cagacgaccg agccgatata gcctttgttg aagaggttgt aatagtcgta
     5221 ggcgattttg ccgcccaaac cgtattccga atcgaggcgg atgccggttt cgagcttgtg
     5281 gcgtttgacc tcggttacgc tgactttgac ggggacgcgg tcgccttgga gacggtcgaa
     5341 gtcggcttgt acggacgcgc cggaataatg cccgttttgt tcgagtgcct gttggaagtc
     5401 gagcagcagg tcgaggtcgt agggcgtgcc cggttggaag cgcgccaggc cggagacgat
     5461 ttgttcgggg tagcgctgtg tgccggtgat ttcaaagtcg ccgaaggcga tggggcggcc
     5521 gctgtccacg acgacgttca aatcgacggt ggcggtatcg gggttgacgg ccgcccgggt
     5581 gttgccgagc ttggcaagcg ggtagccttt gcgcgttacc gcgccgagga cggaagtttt
     5641 gctgttttcc caactgtcct gatcgaaatc actgcctacc ggctgctgcc agttttccag
     5701 cgcgttgcgg tagtattcgg cgaggttgcc gtctgaaagg atgtcgccga ggatggcgac
     5761 accgacgttg gcgattttgg tgcgcgggcc cggtgtgatg tgtaccgtat aagctccgtc
     5821 tttttccgtc aggctgactt tgctgttgaa atagcctttg ctgcggagca tcgttttaac
     5881 gttgtccggc gcttcttcgg caaggaagcc tgtctgttct ttgtccaata cttcttcctg
     5941 ctgctgcgtg atgagcggca ggtgttcttc gaccatatct ttgatttcac tgtcctgcgt
     6001 gtcgatgcgg acgggaaatt tgggttttaa tttgactgat tcggtgtcgg ggcttttgtt
     6061 tttgaacaat gcgaaacccg ccgccttgtt ttcggaaagg tcggcggcag gcgcgtatgc
     6121 gtgcggaaag aaaaataaag ccggcaggag cagggcggtc ggtttgatca tcatggtgcg
     6181 ggtgtcgtgc atcggtgtgg cggttttcga ttctaacact gttcggacgg ggtttcgaac
     6241 ggctgttttt gtgttgttcg gacggggcgg gacgcttcag acggcatact gccgccggta
     6301 tttcggaggt tgcggcccgc ctggaatgta tctgcttgtt ttaaggtatt ttgtttctaa
     6361 tccgcatatg gaaaacaggc tgcgtttcgg aatagtgtcg gaaaataaac gggattaaat
     6421 aaataaattt attttcgttc aataaattaa accgtcgcgc cgaatgataa agcaaagcct
     6481 gtccggcact gcatagggca aatcaacgca accttaaaac acagccgaaa aataaaaaaa
     6541 cacagagaat gagttttctt ttaaaataca acatttttta acatatcaca acataaggaa
     6601 gaattatggc gttcggcaaa tccttatttc atgcgatcgg ccgcgtcagc ctggtcaggc
     6661 agattgccgc cggtttggcg ttgggcatcg taatcggttc ggtttccccg caactgggct
     6721 tggcggcagg cttgttcggc agcctgtttg tcggcgcgct caaagcggtc gcgccggttt
     6781 tggtatttat tttggtggcg gccacaatcg cgcagcacca aaaaggcaac aaggcgcata
     6841 tcaggccgat tatcatcctt tacctcatcg gtacgttttc cgcagccctg accgccgtca
     6901 tcgccggtat ggttttcccg acgcacattg ttttggcggg cgcgggcgat gtgtccgccg
     6961 cgccgccttc cggcattgtg gaagtattga aatcgctgct gatgaacctg gtcgccaacc
     7021 cgattaacgc gattgccaat gccaactata tcggcatttt ggcttgggct ttggttttgg
     7081 gcgcggcgtt gcggaatcac ggttcggacg ttacgcggca ggtcgttgcc gatttggcgg
     7141 aagcggtttc caccgtcgtg aaatggatta tccgttttgc ccctttgggc attttcgggc
     7201 tggtgtcgtc cacaatcgcg gagacgggtt tcggcgcgct ggcggggtac gcgaagctgc
     7261 ttgcagtgct gttgggctgt atggcgttta tcgcgctggc ggtcaatccc gccatcgtgt
     7321 ggtggaaaat ccgccgcaac ccttatccgc tggtgttcac ctgcctgcgc gaaagcggcg
     7381 tgtatgcctt ctttacccgt tcttccgccg ccaatatccc cgtgaatatg gctttggcga
     7441 aaaaactggg gctgcacgaa gacacttatt cgatttccat cccgttgggg gcgaccgtca
     7501 atatgggcgg cgcggcgatt acgattaccg ttttggctat ggcggcggcg cacacccaag
     7561 gcatacaggt tgattttgcc accgcgctgc tgttgagcct ggtggcgacg gtcagcgcgt
     7621 gcggcgcgtc cggcgtggcg ggcggttcgc tgctgctgat tccgctggcg tgcagcctgt
     7681 tcggcatcag caacgatgtc gccatgcagg tggttgccgt cggctttatc atcggcgtga
     7741 ttcaggattc ggcggaaacg gcgttgaatt cttcaaccga tgttttgttt accgctgccg
     7801 ccgatttggg ccgacagaga aaccgggcgg aataaaccaa gctatgccgt ctgaagcccg
     7861 tttggcgttc agacggcata tttttacaat tttatgggca tatggcggaa acaataccgg
     7921 ctgccttgaa tatgaagggg atgatgatgt ttgaacgcag tgtgattgca atggcttgta
     7981 tttttgccct ttcagcctgt gggggcggcg gtggcggatc gcccgatgtt aaatcggcgg
     8041 acacgctgtc aaaaccggcc gctcctgttg ttgttgaaaa agagacagag gtaaaagaag
     8101 atgcgccaca ggcaggttct caaggacagg gcgcgccatc cacacaaggc agccaagata
     8161 tggcggcagt ttcggcagaa aatacaggca atggcggtgc ggcaacaacg gacaaaccca
     8221 aaaatgaaga cgagggaccg caaaatgata tgccgcaaaa ttccgccgaa tccgcaaatc
     8281 aaacagggaa caaccaaccc gccgattctt cagattccgc ccccgcgtca aaccctgcac
     8341 ctgcgaatgg cggtagcaat tttggaaggg ttgatttggc taatggcgtt ttgattgatg
     8401 ggccgtcgca aaatataacg ttgacccact gtaaaggcga ttcttgtaat ggtgataatt
     8461 tattggatga agaagcaccg tcaaaatcag aatttgaaaa tttaaatgag tctgaacgaa
     8521 ttgagaaata taagaaagat gggaaaagcg ataaatttac taatttggtt gcgacagcag
     8581 ttcaagctaa tggaactaac aaatatgtca tcatttataa agacaagtcc gcttcatctt
     8641 catctgcgcg attcaggcgt tctgcacggt cgaggaggtc gcttcctgcc gagatgccgc
     8701 taatccccgt caatcaggcg gatacgctga ttgtcgatgg ggaagcggtc agcctgacgg
     8761 ggcattccgg caatatcttc gcgcccgaag ggaattaccg gtatctgact tacggggcgg
     8821 aaaaattgcc cggcggatcg tatgccctcc gtgtgcaagg cgaaccggca aaaggcgaaa
     8881 tgcttgctgg cacggccgtg tacaacggcg aagtgctgca ttttcatacg gaaaacggcc
     8941 gtccgtaccc gactagaggc aggtttgccg caaaagtcga tttcggcagc aaatctgtgg
     9001 acggcattat cgacagcggc gatgatttgc atatgggtac gcaaaaattc aaagccgcca
     9061 tcgatggaaa cggctttaag gggacttgga cggaaaatgg cggcggggat gtttccggaa
     9121 ggttttacgg cccggccggc gaggaagtgg cgggaaaata cagctatcgc ccgacagatg
     9181 cggaaaaggg cggattcggc gtgtttgccg gcaaaaaaga gcaggattga tgctgatgct
     9241 gtctgaagcg ggggcgttca gacggcattg tgatgtttgg gttgcgggtc cggttcaagt
     9301 ccgcatcagt tgataaaccc gtcgtctttc attttctttt cggaaaaata ctggccgaat
     9361 accagcaagg cggcgaggat gatgacaaac agccccgcca tagtcagtgt gattttgaag
     9421 aacaattccg cttcgagaaa agaaaaccaa agttgcacaa tcagcagcag cacccagccg
     9481 gcaaacagtc cgagacaggc ataaacgcca agcttaaaca cggtcccgct ccaaaatgac
     9541 gacgtaggcc tcgcgtttga aaaacaacag catacggcgc aaatcttttt ccatcgttac
     9601 gacccgccag ccttcggggg tttgtttgtt gaggaacgcg ctgaaattga cggggttgac
     9661 ctttgccgcg ccgaaaaaca ggctggacaa ctggctttcc tgataaatga cgactttgta
     9721 ttctttcata ggattccttt taacataagg gatcat
//
LOCUS       DAXHKX010000060         9723 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHKX010000060 DAXHKX010000000
VERSION     DAXHKX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.060"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            58..1431
                     /locus_tag="ACFMWZ_001121"
     CDS             58..1431
                     /locus_tag="ACFMWZ_001121"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC5935091.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1611..1811
                     /locus_tag="ACFMWZ_001122"
     CDS             1611..1811
                     /locus_tag="ACFMWZ_001122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935092.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALIRKKGYSGLNLNQDKATKPQTV
                     QIVRQGEATPYWFKFNPLNSVY"
     gene            2079..3206
                     /gene="fic"
                     /locus_tag="ACFMWZ_001123"
     CDS             2079..3206
                     /gene="fic"
                     /locus_tag="ACFMWZ_001123"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC5935093.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            3236..4114
                     /locus_tag="ACFMWZ_001124"
     CDS             3236..4114
                     /locus_tag="ACFMWZ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC5935094.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLVVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
     gene            4118..4306
                     /locus_tag="ACFMWZ_001125"
                     /pseudo
     CDS             4118..4306
                     /locus_tag="ACFMWZ_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4383..5894)
                     /gene="lysS"
                     /locus_tag="ACFMWZ_001126"
     CDS             complement(4383..5894)
                     /gene="lysS"
                     /locus_tag="ACFMWZ_001126"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC5935095.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEILDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVNERYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(6049..7302)
                     /locus_tag="ACFMWZ_001127"
     CDS             complement(6049..7302)
                     /locus_tag="ACFMWZ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC5935096.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDVGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            complement(7363..7704)
                     /locus_tag="ACFMWZ_001128"
     CDS             complement(7363..7704)
                     /locus_tag="ACFMWZ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935097.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFLGSNMENIVKFEHEVLIVCVGRYLDYVTIRAFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            7760..9556
                     /locus_tag="ACFMWZ_001129"
     CDS             7760..9556
                     /locus_tag="ACFMWZ_001129"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC5935098.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPSAEQKSDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
BASE COUNT         2458 a         2796 c         2424 g         2045 t
ORIGIN      
        1 ccatcctatc ggtttcccgc ttcagacggc ataaggtctg aaagaaagac tacaattatg
       61 agtaatccat tttcctcttt aggtttgggt acggaactcg tttccgcact gaccgcgcaa
      121 ggttacgaaa acccgacgcc catccaagcc gccgccattc ccaaagcact cgccggtcat
      181 gatttgctag ccgccgcgca aaccggcaca ggcaaaaccg ccgcctttat gctgcccagt
      241 ctggaacgcc tcaaacgtta cgccaccgcc agcacctcgc ccgcgatgca ccccgtgcgt
      301 atgctcgtcc ttacccccac gcgcgaactt gccgaccaaa tcgaccaaaa cgtgcagtcc
      361 tacatcaaaa acctgccgct tcgccacacc gtcttgttcg gcggtatgaa tatggacaaa
      421 cagaccgccg atttgcgcgc cggctgcgaa atcgtcgtcg ccaccgtcgg acggctgctc
      481 gaccatgtga aacagaaaaa catcagttta aacaaagtcg aaatcgtcgt tttggacgaa
      541 gccgaccgta tgctggatat ggggtttatc gacgacatcc gcaaaatcat gcagatgctg
      601 ccccgccaac gccaaaccct gctcttttcc gccaccttct ccgcaccgat acgcaaactg
      661 gcgcaagact tcatgaacgc gcccgaaacc gtcgaggttg ccgcgcaaaa caccaccaac
      721 gccaacgtcg aacaacacat catcgccgtc gataccttcc aaaagcgcaa cctgctcgaa
      781 cggctgattg tcgatttgca tatgaaccag gtcatcgtgt tctgcaaaac caaacaaagc
      841 gtcgaccgcg taacgcgcga actggtccgc cgcaacctgt ccgcacaggc gatacacggc
      901 gaccgttccc aacaaagccg gctcgaaaca ctcaacgcct tcaaagacgg cagcctgcgc
      961 gtcctcgtcg ccaccgacat cgccgcacgc ggcctggaca ttgccgaact gcccttcgtc
     1021 atcaattacg aaatgcccgc ccagcccgaa gactacgtcc accgcatcgg gcgcacgggg
     1081 cgcgcgggcg cggacggcgt ggcgatttcc ctgatggacg aatccgaaca gaaaatgttt
     1141 gaatccatta aagagctgac cggcaacaag ctgctcatcg agcgcatcga gggcttcgag
     1201 ccgcaatggt gggaacaggg cggcgcaaaa ccggaaaaac ccgaaatgcg cgaaccgaga
     1261 caacgcaacc gctacgaatc cgccaaagcg caacgcgaaa aaaacacccg gccggaaaat
     1321 gcggcaaacg atgcgggcgc ggcttgcgga aaaattgccg gacgcagccg ccgaagccgt
     1381 cgggaacacc ggacgtgcgc cctgctccaa ccgcgttacg gcgtaaaata accctgaaaa
     1441 tcaaatgccg tctgaacatt tcccgtttca gacggcattt ttcaaaccgg actgacgcat
     1501 tgggagcaac cgcccgcacc ggataaattt cttccgcaaa cagtttcaga cggcatttgc
     1561 cgcctgtaca atattatttc cttttccact caaaccatca ggatttccca atgtccaccc
     1621 aattcgatgt cgccgtaatc ggcgcaggtc cggcaggttc ggtcgcatcc gccctgatcc
     1681 gcaaaaaagg ttatagtgga ttaaatttaa atcaggacaa ggcaacgaag ccgcagacag
     1741 tacagatagt acggcaaggc gaggcaacgc cgtactggtt taaatttaat ccactaaata
     1801 gtgtatatta agtacgtctg atatacgcga taccctccga gggtgtaaac tttagttcac
     1861 atttaaaatg acctctttaa acctgccttt cggcaggttt ctttttaggt tgtttggaaa
     1921 tcgtgtgcag acaaggtgta aaataggtaa caacataaaa taatgcggtt ttaccgccca
     1981 tatatttaca aaagccaaat ttttaaacat atatccttga tatatacacg gcgtaaatat
     2041 atactggaaa catctttaaa ttttccgaaa ttttaaacat gagcaactgg aaacccgata
     2101 ttccctataa cgatttacca cccctgccgc caaaacagga tattgaaagc aaaaccatcc
     2161 tgaaacgttg tatagccgcc cgtgcatccc ttgcccgttt aaagcaggcg gcagaattga
     2221 taccgaatca agccatgctg attaacaccc ttcctgttat ggaagcccgt gcaagttcgg
     2281 aaattgaaaa catcgtaacc acaacggaca agctgtttca atccctgcaa atggatacgg
     2341 aacggcaaga ccctgccacg aaagaagccc tgcaataccg caccgccctg tttgcaggct
     2401 atgaatcact gacgagccgc cctttatgca cacaaaccgc catcatggtt tgcaacgcca
     2461 tcaagcaccc ctacgaaatg gccatccgca aaacaggcgg cacagcccta aaaggaggca
     2521 acagcggaaa tgttgtctat accccgcccg aaggagaaga aaccatacgc ggcaagctgg
     2581 caaattggga gcggtttatt cacgaaagcg gcgatttaga cccgcttatc atcatggcgg
     2641 cggcacatta ccaatttgaa gccatccacc cgtttacgga cggcaacggg cggacggggc
     2701 gcatattgaa cagcctgcta ttgattgaaa aagggctttt ggatttgcct attttgtatt
     2761 tgagccgcta tattatcgaa aacagggcgg actattaccg cctgctttta ggcgtaaccg
     2821 aacggcagga ctgggaaagc tggataatct acatcttaga cggcgtagcc gataccgccg
     2881 attggacggt agcgaaaata gatgcgatac gccgcctgtt tgagcagaca cgtcaacaca
     2941 tacggacaca cgcacaagga atctacacgc acgaactggt aaatcttctg tttgagcagc
     3001 catatacacg cattgccaac ctagaagcgg cagggatagc caaacggcag acggcctcta
     3061 agtacctgaa agagctttca gacataggtg tgctgcaaga aatcgccatt ggcagggaca
     3121 aactattcat tcatccgcgc ctaatggaac tattgcgggg agagggcaac agctttacct
     3181 cattccaatc cctcgttaaa gcatagccaa aataatcaat aatccggagg tcaatatggc
     3241 aagaaggtca aaaacatttg aagaagctgc tgctgaggtt gaggaacgtt tcggtcatcg
     3301 tggcattaag ttggtcgagt ttgagggtac agccaagccg tgtgtaatca actgccctaa
     3361 acatggaaac caaacctgtt cgaggtactc caatatgttc ataggaagta gctggggttg
     3421 cccctcttgt ggtaatgagc aagctgcaaa agccggtata gcgaccctta ggaagaatca
     3481 catagcgtta gaaatgctga aacaggctgt aacaggtatg accaagcaag agcgcatcac
     3541 gacgcaagcc tacaatgaga tgaccaaatc cgtggcaggt tcaaacagca tagtccttaa
     3601 cgatgtccaa ggcgatacga ccatcaacaa ccatcatacg catacgcaca accacagcga
     3661 tgccgacggc aaagcactgt cgatgaggct cacaccacgc cccctgttgt cagaccgtca
     3721 ggcggcggct ttcgcccgta caggcaaact cacaggcagt ttcgacttgt ttgcttcggt
     3781 ggtcgccccc tcgcagtaca cgtttgccgt tgccatgccc gacacgtcca tgtcgccggt
     3841 tatcgaaaaa ggggatttgg tggtggtcga gccgcgtatg cgccctgcgg acgaggacat
     3901 cgtgctgatt gaactgtccg acaagcggct ggtcgtcgcg caccttgtta tcgatattgc
     3961 gggcaggatg ctgatttatc agacgggcag gccgtctgaa gcctttgacc tgcccgaagg
     4021 cagcacgatt ttaggtgtgg tgctggaggt caaaaaacgg tttatgtccg ccgcacaggc
     4081 gagaaggcgt gttgattcgg attaccaccc ctgatgtgtg gacggttggt acgatttccg
     4141 cttccaaaac gtcgtgtacg cgcccgaccg cagcccggaa atcagccgta tgctttcttc
     4201 gattttggca ggctacgcgt gggataccga aaacccgttc gtggcgaaat ccgaacaacg
     4261 cctgactgcc ttgtccgaat gggtcggtca gttagaaagc gaataaatcc gtaccgccat
     4321 acaaaatgcc gtctgaatcc aatcgggttc agacggcatt gccatttcaa ctgtttttat
     4381 gactactcgg ggcgcatctg cggaaacaga atcacatcgc ggatggtttg cgaatcggtc
     4441 agcaacatca ccaagcggtc gataccgatg ccgcaaccgc cggtcggcgg caaaccaaat
     4501 tccatcgcgc ggatgtagtc ggcatcgtaa tgcatggctt cgtcgtcgcc cgcgtctttt
     4561 tgtgcgactt gcgatttgaa gcgttcggct tggtcttcag ggtcattcaa ttctgaatag
     4621 ccgtttgcca gttcgcggcc gacaacgaac aattcgaaac gttcggtcag accttgtttg
     4681 gtatccgaag cgcgcgccaa cggggaaact tcgaccgggt aatcgatgat gaaagtcggg
     4741 ttccacagct tgccctcggc gcaaccttca aacagcgcga gttgcaggct gccgatgccc
     4801 ggggacggcg gcaggctttc accgtgtttg acgatttctt ttttcagcca ttccgcatcg
     4861 ttcaactgct cgtcggtgta gtgcggattg tattttttga tggcttcgag gatggtcaga
     4921 cgctcgaacg ggctttccaa atcgacttct ttgccattgt aagtgatgtt tgccgtaccg
     4981 tttactgtgc gcgatgcgtt gcggatgatg tcttccgcca tctgcatcat acgttcgtag
     5041 tcggagaagg cttcgtaaaa ttcaatcatc gtaaattcgg ggttgtggcg cacggacatg
     5101 ccttcgttgc ggaagctgcg gttgatttca aacacgcgct ccaaaccgcc gaccaccaag
     5161 cgtttcagat acagctcggg cgcgatgcgc aggtagagcg ggatgtccaa agcattgtgg
     5221 tgggtcacga acggtttcgc cgtcgcgccg ccgggaatcg ggtgcatcat cggggtttcg
     5281 acttcgagat aacgctcgtt caccataaaa ttgcgcacag attggatgat ttggctgcgt
     5341 ttgatgaagg tattgcgcga ttcttcgttg gcaatcaaat cgacgtagcg ttggcggtat
     5401 ttggtttcct gatcgctcaa gcctttgtgt ttgtcgggca gcgggcgcag ggatttagac
     5461 agcaggcgga tgtcggacac gcgtacggtc agttcgccgt ggttggtttt gaacaaagtg
     5521 ccttccgcac cgacgatgtc gcccaagtcc caatggttga agtcgtccaa aatttcttgg
     5581 ctcacgcctt tgttgttcag ataaagctgg atttgcccgg acacgtcttg aatggtggca
     5641 aaactcgcct tgcccatttg acgcttcagc atcatgcggc cggccacttt gacgggaatg
     5701 ccttgcggat cgagttcttc tttgccgatt tcgccgtatt gggcgtgcaa atcggcggcg
     5761 aagctgtcgc gtttgaagtc gttgggatag gcgttgcgct gttggcggat gttgtgcagt
     5821 ttttcgcggc gcagggcgat gatttggttt tcgtccaact gcggctcggt ttgcggatgg
     5881 ttttgttcgc tcatagggtt ttccgaaaaa ataaatcagg cgcaatctgt ttcagacgac
     5941 ctgaccgaat cacaaaattt gcgcgtattt tacgcgatgt cggcattttt ttccataaac
     6001 gcggcaatgc cgtctgaaag cggtttgcgg tttcagacgg catcgttatc atttgaacat
     6061 tcccgccaaa ttcaataaga acaaaacggt aaaaccggtc agataaatca agcctgccaa
     6121 tgcaagggca ttcatacctg atgtgagttt gtgtttttca tcacctttga ccaaacggta
     6181 attcagccag gcaaacacag gggcggacac aaaagcagca atcatcgcaa atttgagcag
     6241 attcgccatt acgccgtcaa accaaaaaat caccgccaaa ccgctgcccg ccacccaaat
     6301 attccaggca aagaattcgg cgttgcccgt tttgtctttt ccgcgcagca ggcgcacggg
     6361 ttcggcaatg gcacgggcat agccgtccac aacggtaatc gtcgtgccgt acatacaggc
     6421 aaacgcgata aacgccacca gcgggcgcga ccagccgccg atggtaacgg cgtacatatt
     6481 gatcaattgc ccgatatatt tgccgcccgc catctgcact gcttcgccgt tgccgtattg
     6541 cacaaacgca cccagtgcaa ggaaaaccaa agccaaaacc gcactggcga tataaccgac
     6601 gttgaaatca aaaatcccgt cgcggtattc ggaaggattg atgcgttgtt tttcggttac
     6661 ccacaaagaa ttgatggcgg aaatttcaat cggcgcgggc atccagccca tcagcgcgat
     6721 caggaagccc aaaccggcaa gcgtccacgg tgtcggctcg ataaaatcgg gctgcatctg
     6781 cataccgcgc gacatagcga tgccggcggc ggcaagcgtg gcgatactca aagtaacgat
     6841 gatgattttg gaaacgcgat ccaaagcgcg gtaacgtccg ctcaccaaaa taatcaggca
     6901 ggatgccata atcaaggcgg caaccgtgcc gacatcaaac atcagcgagg gaatcgccat
     6961 tttgacgatg gcggcggtta caatggcgac cgcgcccgcg ttaatcgtgg cggagaggat
     7021 gcacaaaatc aggaataccc acaaataaac gcggcttttc tcggcataac cttcaatcag
     7081 gctcttgccc gtgtccagcg tgtaatgcgc gctgaagcgg aaaaacgggt atttgaagag
     7141 gttggtcagg atgatgatga gcgcgatctg ccagccgtaa agcgcgcccg cctgcgtcga
     7201 ggcaatcagg tgcgaaccgc cgaccgccgc cgaagccatc ataatccccg gtcccaatgc
     7261 gttgatttta cttttccaag tcgaaatatg ttgttcggac ataaagtctt ccgtattttt
     7321 aaccgtgttt caacacacag agccgcatat tcggacacag ccctatctat tgctccaatt
     7381 tgggcgtgat tgcccccaaa caaacccaaa tcctaccgtc ttcaaaaaca ggataccgcc
     7441 cggtagggaa attttgatga aagcgcgtat tgtaacgtaa tccaaatacc tgccaacaca
     7501 cactattaga acttcatgct caaacttgac tatattttcc atattacttc ccaaaaaagg
     7561 catgaaacga cattttatgc ctaaaatttt acaacaaaca accttacatc gcttttttcg
     7621 cgcaaacacg caccatccga tcagcccgtc cgttttgcag caggctggcg atttgataag
     7681 atggttatgt ttttcagacg gcatttcaga tttccgtcca tgccgtctga agccgcaaaa
     7741 cccgattgga ggaactgtta tgaataccgt atcgaattac ctgtccgcat tgcgcgaagc
     7801 catgaaggcg caaggcttgg atgcactcgt catcccttcc gccgaccccc acctgtccga
     7861 atacctgccc gcacactggc aggcgcgccg cgaattgtcg ggctttaccg gctcggtcgg
     7921 cacatttgtc gttaccgccg acgaagcggg cgtgtgggtg gacagccgct attgggaaca
     7981 agccgccaaa cagctttccg gcagcggcat tgagctgcaa aaaagcgggc aagtgccgcc
     8041 gtataacgaa tggctcgcgg caaacctgcc cgaaaacgcc gccgtcggca tcccttccga
     8101 tatggtctcg ctcaccggca aacgcacttt ggcgcaatcc ctgaccgcca aaaacatccg
     8161 catcgaacac ccaaacgatc tgcttgacca agtgtggaca agccgcccgg ccattcctgc
     8221 cgaaaccgtg ttcatccacg accccgccta cgtctccgaa accgccgccg aaaaactcgc
     8281 ccgcgtgcgc gccgtgatgg cggaaaaagg cgcggattac cacttggttt cctcgcttga
     8341 cgacatcgct tggctgacca acctgcgtgg cagcgacgta cctttcaacc ctgttttcgt
     8401 gtccttcctg ctgattggca aagacaacgc cgtcctgttt accgaccgat gccgtctgaa
     8461 cgccgaagcc gccgccgcgc tgcaaaccgc aggcatcacg gtcgaacctt atgcccaagt
     8521 tgccgacaaa ctcgcgcaaa tcggcggcgc gctgcttatc gaaccgaaca aaaccgccgt
     8581 cagcacgctt gtgcgcctgc ccgaaagcgt gcgccttatc gagggaatca acccatccac
     8641 gctgttcaaa tcctgcaaat ccgaagccga catcgcccgc atccgcgaag cgatggaaca
     8701 cgacggcgcg gcgttgtgcg gtttcttcgc cgagtttgaa gacatcatcg gcaacggcgg
     8761 cagcctgacc gaaatcgacg tggacaccat gctttatcgc caccgcagcg tgcgcccggg
     8821 cttcatttca ttgagtttcg acaccatcgc aggcttcaac gccaacggcg cactgccgca
     8881 ttacagcgcg acacccgaaa gccacagcac catcagcggc aacgggcttt tgctcatcga
     8941 ctccggcgcg caatacaaag gcggcacgac cgacatcacc cgcgtcgtcc ccgtcggcac
     9001 gccgagtgcc gaacaaaaaa gcgacaacac cctcgttctc aaagcccata tcgcgcttgc
     9061 cgaagccgta ttccccgaaa acatcccctc gccgctgatt gatgcgattt gccgcaaacc
     9121 cctgtggcag gcgcaatgcg actacggcca cggcaccgga cacggcgtag gctatttcct
     9181 caacgtccac gaaggcccgc agcgcatcgc cttcgccgcc cccgccacgc ccgaaaccgc
     9241 catgaaaaaa ggcatggtta cctccatcga acccggactc taccgcccag gaaaatgggg
     9301 catccgcatt gaaaaccttg ccgccaacca agccgtcgcc gcccctcaag aaaccgaatt
     9361 cggcagcttc ctctgttttg aaaccctgac cctctgcccc atcgacaccc gcctgatgga
     9421 caccgccctc atgaccgacg gcgaaatcga ctgggtcaac cgctaccacg ccgaagtccg
     9481 ccgccgcctc gagccgctga ccgaaggcgc ggcaaaagcg tggctgatca aacgcaccga
     9541 accgctggcg cgttaaacag cacggcgcaa aaaatgccgt ctgaaagccc ttcagacggc
     9601 attggtttcc caaaacatcc cgcaccgttt tcatcttgcc gcaagcaaat atagtggatt
     9661 aacaaaaatc aggacaaggc gacgaagccg cagacagtac gaatagtacg gaaccgattc
     9721 act
//
LOCUS       DAXHKX010000061         9650 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHKX010000061 DAXHKX010000000
VERSION     DAXHKX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.061"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(312..545)
                     /locus_tag="ACFMWZ_001130"
     CDS             complement(312..545)
                     /locus_tag="ACFMWZ_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC5935099.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(563..697)
                     /locus_tag="ACFMWZ_001131"
     CDS             complement(563..697)
                     /locus_tag="ACFMWZ_001131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935100.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLENIVFSQIDHDSAASKALACAN
                     "
     gene            complement(711..911)
                     /locus_tag="ACFMWZ_001132"
     CDS             complement(711..911)
                     /locus_tag="ACFMWZ_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC5935101.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="ACFMWZ_001133"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="ACFMWZ_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC5935102.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTCLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="ACFMWZ_001134"
     CDS             complement(2010..2450)
                     /locus_tag="ACFMWZ_001134"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC5935103.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="ACFMWZ_001135"
     CDS             2771..3709
                     /locus_tag="ACFMWZ_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC5935104.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3838..4131
                     /gene="hfq"
                     /locus_tag="ACFMWZ_001136"
     CDS             3838..4131
                     /gene="hfq"
                     /locus_tag="ACFMWZ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC5935105.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            4215..4781
                     /locus_tag="ACFMWZ_001137"
     CDS             4215..4781
                     /locus_tag="ACFMWZ_001137"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC5935106.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            4835..5209
                     /locus_tag="ACFMWZ_001138"
     CDS             4835..5209
                     /locus_tag="ACFMWZ_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC5935107.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5219..5713
                     /gene="folK"
                     /locus_tag="ACFMWZ_001139"
     CDS             5219..5713
                     /gene="folK"
                     /locus_tag="ACFMWZ_001139"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC5935108.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(5838..6317)
                     /locus_tag="ACFMWZ_001140"
     CDS             complement(5838..6317)
                     /locus_tag="ACFMWZ_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC5935109.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHMAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(6537..7274)
                     /gene="ubiE"
                     /locus_tag="ACFMWZ_001141"
     CDS             complement(6537..7274)
                     /gene="ubiE"
                     /locus_tag="ACFMWZ_001141"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC5935110.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7317..7685)
                     /locus_tag="ACFMWZ_001142"
     CDS             complement(7317..7685)
                     /locus_tag="ACFMWZ_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC5935111.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7805..9424
                     /gene="mnmC"
                     /locus_tag="ACFMWZ_001143"
     CDS             7805..9424
                     /gene="mnmC"
                     /locus_tag="ACFMWZ_001143"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC5935112.1"
                     /translation="MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQAMQCLQFDSINLIEHILPDVRFWLVPPSRTHHLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLATLPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRAIIRAIVRRKDLTP"
     gene            complement(9538..9650)
                     /locus_tag="ACFMWZ_001144"
                     /pseudo
     CDS             complement(9538..9650)
                     /locus_tag="ACFMWZ_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         2587 a         2959 c         1937 g         2167 t
ORIGIN      
        1 taccgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagtaaaaca
       61 tgatatgatt gccaacaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggttaat atgcaacatc
      181 gaagaagact agcaatttac caagcatcca aacgtgcttc ctttaccggc aggtcatcag
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgtctgaagt cctgcttcag
      301 acggcatttt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatt ttgaaaaggt
      481 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt caaggaagat gatactcaag tcctgccaaa
      661 aacagcaaaa atgccgtctg aacagttcag acggcatttc caaaaccgtt ttacgcttga
      721 acattgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccac agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgcg aatataaata aaaacggttc gcattgcaag gtcggtatac acggtttgtc
     1021 ttggtaattt tttatcaagt ttgcattttc gattgatttc ttattaaacc aaagtagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacgtt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaagccgt gccgcaccag atgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgcctgact tgcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atgcccgtct ttttctgact gacaaacaat gcgtcgcaat tcctgccttt cagcagaagt
     1381 gggcgtgcct ccgtataata gcgttccacc caatacgccg actcctgccc catcgggacc
     1441 atcctctgct tatcaccctt tcccagcgcg gtaatacagc ccctgtccaa atccacattg
     1501 ccgaagttca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
     1561 aaagccttat cccgcaaacc gtgcggcgtg tcggtatccg gggcggcaag cagtcgggaa
     1621 atctgctgct cggtgatcag ggtcggaata ttcttgtcga ttttgggcgg tttcagcagg
     1681 caggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcac
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcgtt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgac tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccggcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacggcacgc agaggcaccg ccgcaattgc caaaccaacc
     2521 ccgattctac cgccccaaag gacaaggatt caactgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacaataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ccgacgaaat cgaccgcctc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaga actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgtaaagccc aaagcctcgg tatgtacggc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttctaccgc caaagacctg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgccctggtc agagaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaat caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa attggtaatt tttcctattc aaggctacaa
     3781 ttcgacacgc acagggcgtc tgcccgattt cacaatcata ataacggagt taaaaatatg
     3841 acagctaaag gacaaatgtt gcaagatccc ttcctgaacg ccctgcgtaa agagcacgtt
     3901 ccggtttcga tttacctggt caacggcatc aaactgcaag gtcaggttga atctttcgac
     3961 caatacgtcg ttcttctgag aaacacttcc gtcacccaaa tggtttacaa acacgccatt
     4021 tccaccatcg taccggcacg ctccgtcaac ctacaacatg aaaacagacc ccaagccgca
     4081 ccggcttcga ccctcgtcca agtggaaacc gtccagcagc ctgccgaata atccgcacga
     4141 agcatgacgt gtcatatctt tcaatacctt accgacatcg gtaaggtatt tttattttca
     4201 gacagcattt aaaaatgtta ttgcaaaaca tccttccatt cgcccattgc cttttgcgga
     4261 aggcacttcc cgaaggcggc aatgctttgg acggcaccgc cggcaacgga cacgacaccc
     4321 ttttcctcgc acaaaccgca ggcatccggg ggaaagtgtg ggcattcgac atccagccgc
     4381 aagccctgag ccgcactcga tgccgtctga aagaagcgag atacgagaat gtgcaactca
     4441 tcttagacgg acacgaaaac ctgaaacaat atgtttcaga accactaaat gccgccattt
     4501 tcaactttgg ctggctgccc ggcggagata aaagccttac cacccatacg gaaaccagca
     4561 ttcccgccct ttgcgcaacc ctctccctgc tgaaagaagg cggtatgctt attgccgtcc
     4621 tctatccggg acacgaaaac ggcaaacagg aggcagaagc aatcgaacaa tgggcaaaaa
     4681 acctgcctca agaacagttt gccgttttgc gttacggctt taccaaccgg aaaaacagcc
     4741 cgccctatct tttggcattt gaaaaactgc gtcaaaaata actgtttgcg gtaaaataag
     4801 ccgattctaa cccttatttt gatacgagcc gaccatgcag tatctgattg tcaaatacag
     4861 ccatcaaatc ttcgttacca tcaccatttt ggtattcaac atccgttttt tcctactttg
     4921 gaaaaatcca gaaaagccct tggcgggctt ttggaaagca ctgccccacc ttaacgacac
     4981 catgctgctg tttacgggat tgtggctgat gaaaattacc catttctccc cgttcaacgc
     5041 gccttggctc ggtacaaaaa tcctgcttct gctcgcctat atcgcattgg gtatgatgat
     5101 gatgcgcgcc cgtccgcgtt cgaccaagtt ctacaccgtt tacctgctcg ccatgtgttg
     5161 cgtcgcctgc atcgtttacc ttgccaaaac caaagtcctg cctttctgaa acaccgttat
     5221 gaacaacaga cattttgccg tcatcgccct gggcagtaat cttgaaaacc ctgcccaaca
     5281 ggtacgcgcc gcattggaca cgctgtcgtc ccatcctgac atccgtctta aacaggcttc
     5341 ctcactgtat atgaccgcgc ccgtcggtta cgacaatcag cccgattttg tcaatgccgt
     5401 ctgcaccgtt tccaccactc tggacggcat tgccctgctt gccgaactca accgtatcga
     5461 ggctgatttc ggacgcgaac gcagcttccg caacgcgccg cgcacattgg atttggacat
     5521 tatcgacttt gacggcatct ccagcgacga cacccgactc accctgccgc atccgcgcgc
     5581 gcacgaacgc agtttcgtca tccgcccttt ggcagaaatc ctccctgatt ttgttttagg
     5641 aaaacacgga aaggttgccg aattgtcaaa acggctgggc aatcaaggta tccgtctttt
     5701 accggacagg taattccgca cgcggatgcc gtctgaaagc ctttcagacg gcatttttcc
     5761 tttgccgcca acacgcgtgc aaaaaaatcg ccccttggaa aaggggcgca aaaggaacac
     5821 aaaccactac caaaacttta aatctgaaac actgcctgcc gcatactgta tccgacagga
     5881 tataaagccc tcactaaatc gtttcgagaa atccaaactt cttcatcgcc gacagaaaat
     5941 ctgcctttct ccggcaccag ctccaacaaa aacggttgaa ccgccatatg cagcctgtcc
     6001 ttacaccgcc ccagctttct agacaacgcg gacaaggcac gcttatagac actatccttg
     6061 cggtcaagtt cccgcgcact gccgacccag ctcaaacgaa ggttgcggct ttcatcctca
     6121 atcagcaaaa tgcctgtttt gacttccccc acctcgggct cgcatgaaag cgcaatataa
     6181 tatttgccgt cgatgttgac atacgccgct tcatgcacct gacagttgca gccagaagtg
     6241 gcaagcagcg aataaacaga gtcccgatgc agtttcaaaa tatcaaggga aatctgtttg
     6301 aaaatacttt ccggcatttt tccctccttt cgtctgctgc gttgtttact ggattgactg
     6361 cattatacag acatctccca atcttgcaaa tgataattgt tcctattttt taaaaaattt
     6421 ataaattatt gatggatatg tgaattaatt ttaagattta aaatctcttt ctcaaaaaaa
     6481 cacaaaaatg ccgtctgaaa cgtgttcaga cggcattggc tgcacagcca gtccgtttaa
     6541 aatttcacgc ccttatgcag cgcgacgatg cccgcactca tattgtgata atccacgctg
     6601 tcaaagcccg catccaacat catctgtttc aaagtttcct gatcggggtg catacggatg
     6661 gactcggcga gatactggta gctgtccgca tctttggcaa tcagtttgcc catgaccggc
     6721 agcagcttga acgaatagaa atcatacgcg ccttccaaag gtttgtagat tttggaaaac
     6781 tccaatacca gcaatgtccc gccgggtttc aaaacacggt acatctcttt cagcgcggca
     6841 tctttatgcg tcatattccg caaaccgaac gccacggaaa ccaagttgaa ataattgtca
     6901 gggaaaggca gtttttccgc atcggcaagc gacacgggca aaatcatgcc ttcgttcaaa
     6961 agacggtcgc gcccgacggt cagcatagag gaattaatat cggtcagcca aacctcgcct
     7021 tccttgccga cccgtttcgc ccaaccgcgc gacaaatcgc ccgtaccgcc ggcaatgtcc
     7081 aacactttat cgcctttttt caggtgcgcc gtgttgatgg tgaaatgctt ccacaccctg
     7141 tgcagccctg ccgacatcac atcgttcata atgtcgtagt ttttggcgac ggagtggaac
     7201 acttccgcca cttttccggc tttctcatct tcgttgaccg tactgaatcc gaaatgcgtt
     7261 ttctgtccgc ccatattcta accacctccc tgatgccgcc ctacggcatc ccaaacttaa
     7321 caatctgatt gcttttcaaa acgggaagca cccgccgccg ccaatttgtc caaatattcc
     7381 tgccacatcg catcgtatcc gtatgccagt ctgtgcagat acgcccaatc gtaaagaccg
     7441 ctgtcgtgcc cgtctgaaaa actgattttc agcgcgtact gtccgacagg ctgcaaatcc
     7501 gcgatttgga catccgcctt gccggtctgc aaaacatcct gtcccacgcc gtgtccgcgc
     7561 acttccgcac tcggcgaata gacgcgtaaa aattcggcag gcagattttt aggttcgtcc
     7621 ccataagtca aaaccaacac ggtacggttt ttcaacaaac ggatttcaac aggaatatta
     7681 ttcaaaaaac acctcggttc ggcatacaat atccggcagg cgcaggcgtg ccttcaaacg
     7741 gcgcgtattt taacagcttt atcccgatac cgatattcaa acccacactt taaacggcat
     7801 tgatatggac aatctcgtat gggacggcat tcccgacatc cgcacactcg accaagccat
     7861 ccgcaaacac gcacccccgc tcaacctgat tatctgcctc cccgataatc agattcccga
     7921 ttttcaaacc gcacaagatg cttcggacgc ggaatgccgt ctgaagcacc gtttggatca
     7981 ggcaatgcag tgcctccagt tcgacagcat caacctcatc gaacacatcc tgcccgatgt
     8041 ccgcttctgg ctggttcccc cttcacgcac ccaccacctg cacgaacatt tccaccacat
     8101 ttcctggcag accgaagcca tcccgcaaac cgaaagcaag cccgacaaac cctggtttgc
     8161 acttccacaa acatccgaac ggcaaaaacc ggaacacatc ctcgttatcg gcgcgggcat
     8221 atccggcgcg gcaaccgccc acgccttagc atcacacggc atttccgtta ccgtattgga
     8281 agcccgaaaa gccgcccaag ccgccagcgg caaccgccaa gggctgctct acgccaaaat
     8341 ctcgccgcac gacaccgaac aaaccgaact gctgcttgcc ggctacggct acaccaaacg
     8401 cctgctcgga catatcctgc ccgaatccga aacctggggc ggcaacggca tcatccacct
     8461 caattacagc cgcaccgaac aacaacgcaa tcacgaattg ggtttgcaaa aacaccataa
     8521 ccacctctac cgcagcatca cgcaggcaga agccgaaaaa atcgccggta tcccactgtc
     8581 cgtcccatac gaccaccctt catgcggact ctactggcaa cacggcgtat ggctcaatcc
     8641 acccgcattc gtccgcaccc tcctcaacca tccgctcatt ggactacacg aagacacacc
     8701 gttaaccgac atttcccacg acggggaaaa gtggattgca agcacgccaa acggcacatt
     8761 taccgccaca cacatcatct actgcaccgg tgcgaacagc ccctacctac ccgaaaccaa
     8821 cctcgccacc ctgcccctca ggcaaatacg cggacaaacc ggcctcacac cgtccacccc
     8881 gttttccgaa caactgcgtt gcgccgtttc aggcgaaagc tacatcagcc cgtcgtggca
     8941 cggactgcac tgctacggcg cgagttttat tcccaacagc agccataccg gatggaacga
     9001 agccgaagaa gcctcaaacc gccaagcatt ggcacacctt aaccccgccc tttccgaatc
     9061 attgtttgcc gccaacccaa acccccaaaa acaccaaggg cacgccgcca tacgctgcga
     9121 cagccccgac caccttcccc tagtcggcgc actcggcgac attgccgcta tgcaacaaac
     9181 ttacgccaaa ctcgcgctgg acaaaaacta ccgcatcgat gccccctgcc cgtacctgcc
     9241 caatgcctac gccaacaccg cccacggcac acgcgggctt gccaccgccc ccatctgcgc
     9301 cgccgccgtt gcagccgaaa tcctaggctt gccccatccc ctctcaaaac gcctgcgcca
     9361 cgccctacac cccaaccgcg ccatcatccg cgccatcgtc agaaggaagg atctaacccc
     9421 ttaagccttt gccgcatgcc gtcccgacca cagaaaatca tattcaaata aacataccaa
     9481 tcctgacggt aaaatatcga aaacacccat aacattttct tcccaaaata aggagtaagg
     9541 aatgcctaac aaattgcctt tgctgtccaa gatgaatgtt ccgtaacatt cgaagacttc
     9601 cttaaccctg ccagccgttt cggtttctgt accccttgtt agataaggaa
//
LOCUS       DAXHKX010000062         9598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHKX010000062 DAXHKX010000000
VERSION     DAXHKX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.062"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(113..1555)
                     /gene="aldA"
                     /locus_tag="ACFMWZ_001145"
     CDS             complement(113..1555)
                     /gene="aldA"
                     /locus_tag="ACFMWZ_001145"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC5935113.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1590..1790)
                     /locus_tag="ACFMWZ_001146"
     CDS             complement(1590..1790)
                     /locus_tag="ACFMWZ_001146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935114.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1799..2188)
                     /locus_tag="ACFMWZ_001147"
     CDS             complement(1799..2188)
                     /locus_tag="ACFMWZ_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935115.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2189..2365)
                     /locus_tag="ACFMWZ_001148"
     CDS             complement(2189..2365)
                     /locus_tag="ACFMWZ_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935116.1"
                     /translation="MSGYVQFLGTDSKGQSKFIFVGTNENGSITTIHTKSGKDFWRTL
                     NNNPKNKTIYPKAR"
     gene            complement(2396..3313)
                     /locus_tag="ACFMWZ_001149"
     CDS             complement(2396..3313)
                     /locus_tag="ACFMWZ_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC5935117.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYMEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLNFGINRSRFQIDADGGKVILISVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYACFTPILYREPVRIWNLSPNLRGLAASCLQAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFNRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3486..4286
                     /locus_tag="ACFMWZ_001150"
     CDS             3486..4286
                     /locus_tag="ACFMWZ_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC5935118.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4334..5110
                     /gene="mlaE"
                     /locus_tag="ACFMWZ_001151"
     CDS             4334..5110
                     /gene="mlaE"
                     /locus_tag="ACFMWZ_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC5935119.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5161..5655
                     /gene="mlaD"
                     /locus_tag="ACFMWZ_001152"
     CDS             5161..5655
                     /gene="mlaD"
                     /locus_tag="ACFMWZ_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC5935120.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5692..6282
                     /locus_tag="ACFMWZ_001153"
     CDS             5692..6282
                     /locus_tag="ACFMWZ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFC5935121.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6339..6617
                     /locus_tag="ACFMWZ_001154"
     CDS             6339..6617
                     /locus_tag="ACFMWZ_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC5935122.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6614..7441
                     /locus_tag="ACFMWZ_001155"
     CDS             6614..7441
                     /locus_tag="ACFMWZ_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC5935123.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7566..7928
                     /locus_tag="ACFMWZ_001156"
     CDS             7566..7928
                     /locus_tag="ACFMWZ_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC5935124.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7928..8311
                     /locus_tag="ACFMWZ_001157"
     CDS             7928..8311
                     /locus_tag="ACFMWZ_001157"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC5935125.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEELGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8308..8796
                     /locus_tag="ACFMWZ_001158"
     CDS             8308..8796
                     /locus_tag="ACFMWZ_001158"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC5935126.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            8858..8992
                     /locus_tag="ACFMWZ_001159"
     CDS             8858..8992
                     /locus_tag="ACFMWZ_001159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935127.1"
                     /translation="MFGFRRHFIFRRFFTVAGLPEAKGRGMPFAGAPEPRAGTMPLRP
                     "
     gene            9305..9520
                     /gene="rpmE"
                     /locus_tag="ACFMWZ_001160"
     CDS             9305..9520
                     /gene="rpmE"
                     /locus_tag="ACFMWZ_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC5935128.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         2208 a         2653 c         2412 g         2325 t
ORIGIN      
        1 agacaaaaac atcgatgtcg gtcatgactg attgccggtg aaactgtaaa aatgccgtct
       61 gaacctgaaa acgggttcag acggcatttt ctatcggggt tttaaagcgg cattaaatgt
      121 cggtttccag ataaacgact tgggtttgca gatattcttc caaaccgtgt ttgccgtccg
      181 cgccgccgat gccggatttt ttccagcccg cgtggaaacc ctgcatggct tcgaagtttt
      241 cgcggttgat gtaggtttcg ccgaattgca ggcggcgggt aacgtagaag gcttcgttta
      301 aattagtcgt ataaacagaa ctggtcagac caaactcgca atcgttagcc agtgcgatga
      361 cttggtcgag cgtgtcgaaa gcggaaacgg gcagcacggg gccgaaggtt tcttctttca
      421 taatgtccat actgttgtcg gtgtcggtca gcagggtcgg ctcgaagaaa tagccgcgcc
      481 cttcggcgcg tttgccgccg cagaccagtt tcgcaccttg tttgactgcc cgttccactt
      541 tttcggcaac ggctttgacg gcgcgctctt caatcagcgg acccatttcc agcgcgcctg
      601 cttcggcttc ggcagggttg ccgtagcgca cgcctttcat ggcggcggtc attttttcaa
      661 tgaacgcgtc tttcaggctg ctgtggacat agacgcgctc ggcgcagttg cagatttgac
      721 cggtgttgcc gacgcgcgaa gccaagatgg atttcaccgc caaatccaaa tccgcgtctt
      781 tcaaaacgat ggcgggggct ttgccgccga gttccagcga aactttggtg atgttggcgg
      841 aagcggcttc catcacttgg cggcctgctt cgacggagcc ggtcaggctg accatatcga
      901 cttgcggatg ggcagacaag gcattgccga tttccgcacc cggaccgttc accacgttga
      961 acacgcctgc gggcaatccg accgcatcga cgatttcggc gaagatgtgg cagttgatcg
     1021 gggttacgct gctgggtttg acaacgatgg tgttgcccgt tactaaggcg ggacccattt
     1081 tgcgggcaat caggaagaaa gggaagttcc acggcaaaat gccggcaatt acgcccagcg
     1141 gacgtttgaa caataaaatg ttttcgcgcg ggcggtcgct ttggatgatt tcgccttcgt
     1201 aacggcgcgc ccattcggcc tggtaatcga gatagtcggc ggtaaacatg acttccacgc
     1261 gtgccaagtc tttggttttg ccaccttcgg caacgatggt gtcggtcagc tcgtcggcac
     1321 gttcgcgtat gccttgggcg attttgcgca aatacgcgcc gcgttcgacc gcaggcagac
     1381 gttcccaagc cggttgcgcc gcgcgtgccg ccgccacggc tcggtcaacg tccgctttgc
     1441 cgcctttggg ttcgcgggcg atggtttctt cggtggacgg gttcaatacg tcgcgccatt
     1501 cgccgttgaa gtcgttttca aagcgtccgt tgatgtacat ggccaattgt ttcatttcgg
     1561 gttctccagt tttgtagtca gatgtagttt tagtttattc ccaaataaat taccgtacaa
     1621 gtttcttttg aaatgtttag acgagaagct actatgtctg catattgttc tgctaattta
     1681 tattcactcg aatgcaactg ccgattattc caatctgcat tcgcaccagt aaaagcccca
     1741 gcattgccgc ctgtggcata accaatcgct aaactgccaa cggtatccac aacaggtttc
     1801 aggctgcctg aatttctcta ataaatgaaa cacttattct atcagccgtt aatgtaacca
     1861 aacaattatc aagataagag tagtttatta aatactcatc ttcttcaaat tgtatatttt
     1921 gtagggttag atgattattt tttaattctg ccgttatttc atacttgaaa ctatcatcta
     1981 tttttctaaa tccaatattt gaagttgatt tatccagtat gtaatcatta aagatttcat
     2041 aggataattc cacttcataa aattgatttt caaatatttt gaatgggcag tacgatataa
     2101 aacaatgtag ttcataacca tttatttcaa tcaatactgc ctcttcaatg gtgttatcta
     2161 actttttgat tttacattta tataacattt atcttgcctt tggataaatt gttttgtttt
     2221 ttggattgtt attcaaagtt ctccaaaagt cttttcctga ttttgtatgt atcgtggtaa
     2281 tactaccatt ttcattggtt ccaacaaaaa taaatttaga ttgacctttg gaatcagtac
     2341 ccaaaaactg aacatatcca gacatgcgga tttcgggttt taaatcaaat gcggcttacc
     2401 ggttttcttt tttggtttcc gcaggcgtgc tgccgaaata ttgcctgaat gcctgagtaa
     2461 agctggaaac gtggcggtag ccgcacaaat acgcggtttc gcctatgctt ttgcccccgt
     2521 tttgcaacag gtagagcgcg tgctgcattt gtttgtggtg cagccactcg cttgcggtaa
     2581 taccgaaatg gtcgcgcata cggcgttgca gcgtcctttc actgatgttc agcgcgtctg
     2641 tcagccggtt gacttggtgt gcgccgtcgg caaacgcggc atttagggtg cggttgaagt
     2701 cttcagacgg catagcgtct gcttccgccg tttgccccgc cgccggcccg atgccgtctg
     2761 aaaccgtgtc ccacaagtcc gacagcagcc gcaaaacgtc cgcctcgcgg cgcaatgttt
     2821 cgcccaaatg cccctttggg acggcttgca ggcaggatgc cgccaaaccg cgcaggtttg
     2881 gggacaaatt ccatattctg accggttcgc ggtaaagtat gggtgtaaaa cacgcatact
     2941 ccggacgcag cagccattgc tccatacctt taattgtcat tttgaccgtt ttgccgcctc
     3001 gataaagata gcggctaaac aggacttctt ccccgactga aatcaggatg accttgccgc
     3061 cgtccgcatc gatttggaat cggctccggt tgatgccgaa gttcaaagag ccttccagca
     3121 aaagcacgaa cgacacataa ggttcggcga ggcggctgct gcaaaaatcg caccgtgccg
     3181 ttaccgtgcc gccgtggatg gaaatgccgt tggacaaagt atcgaaacgg tagctgccct
     3241 ccatatagct gccgccgccg cttttgacca gattgacgaa ccggctgctg ttcaaatgtt
     3301 ctgctttttc catagttttc tgataaaggt tatcatttga aagataacat ttttcgccac
     3361 agcaaacaat ccgccgccga caacggcatt ttcacaacac ttaccgtccg tatgccgtct
     3421 gaaaattgcc cgtcttcccg ataagcctta aaatacacgc cgtcaaacct accgtccgtc
     3481 cgtacatgag cccatccccc tttatcgaaa tgaaagacgt cgccttcgcg tatggcgacc
     3541 gcccgattct gaacgacatc aatttcagta ttccgcaagg caattttgcc gccgtgatgg
     3601 gcggttcggg cagcggcaaa accacgctga tgcgactgat tacaggacag attcgtccgc
     3661 agtccgggca ggttttgatt gaaggacgtg atttgtcggg cttttcggct gacgagttgt
     3721 atgaacaccg ccgccgtatg ggcgtattgt tccaacacgg cgcgctgttt accgatttgt
     3781 ctgtatttga caatattgct tttccgatgc gcgaactgac gcaactgccg gaagcggtga
     3841 ttcgagattt ggttttgttg aaactgaacg cggtcggtct gcgcggtgta gaaaacctga
     3901 tgccgtccga gttgtccggc gggatgtcgc gccgcgtcgc gcttgcccgc acgattgcgc
     3961 tcgaccccga aattatgttg tacgacgaac cgtttaccgg tctcgatccg atttccttgg
     4021 gcgtgattgc ccacttaatc agccgcgtca acaaggcttt gcgttcgacc agtattatgg
     4081 taacgcacga cattgaaaaa tctttggaaa tcgtcgatca ggtgattttc ttggcgcacg
     4141 gcgaaattat gttctccggc tcgccgcagg aaatgcgcga actggattcg ccttgggtgc
     4201 gccagtttgt cggcgggctg gcagacggcc ccgtagcata ccgctatccg gcgcaaacgt
     4261 cgttgcagca ggatttgctc gggtaaatga gcaggtgccg tctgaaaccg ttgaacttta
     4321 catatagaaa tttatgaatt ttatccgttc cgtcggggcg aaaaccctcg gccttattca
     4381 atccctcggc agtattacgc tgtttctgct gaacattctg gcgaagtccg gtacggcttt
     4441 cgtccgtccg cgcctgagcg tgcgccaagt gtatttcgcc ggcgtgctgt cggtgctgat
     4501 tgttgccgtt tcggggctgt tcgtcggcat ggtcttgggt ttgcagggct atacgcagtt
     4561 gtcgaaattc aaatccgccg atattttggg ctatatggtt gcggcttcgc tgttgcgcga
     4621 actgggtccg gtgttggcgg cgattctgtt tgccagcagc gcgggcggtg cgatgaccag
     4681 cgaaatcggt ttgatgaaaa cgaccgaaca gctcgaagcg atgaacgtga tggcggtaaa
     4741 ccccgtcgcc cgagtggttg cgccgcgctt ttgggcgggc gtgttttcca tgccgctttt
     4801 ggcttcgatt ttcaacgtgg cgggtatttt cggcgcgtat ttggtcggtg taacctggct
     4861 gggcttggac agcggtattt tctggtcgca aatgcagaac aatatcacga tacattacga
     4921 tgtaatcaac ggtctgatta aatccgccgc gttcggcgtg gcggtaacgc tgattgccgt
     4981 gcatcagggc ttccattgcg tcccgacttc ggaaggcatt ttgcgcgcca gcacgcgcac
     5041 ggtggtttcg tccgccctga cgattttggc ggtcgatttt atattgaccg cgtggatgtt
     5101 tacagattga caggccgtct gaagacgaaa cgcggaacat cggatattca aggaacttta
     5161 atgaaaaaga acatattgga attttgggtc ggactgttcg tcctgattgg cgcggcggcg
     5221 gttgcctttc tcgctttccg cgtggccggc ggtgcggcgt tcggcggttc ggacaaaact
     5281 tacgccgttt atgccgattt cggcgacatc ggcggtttga aggtcaatgc ccccgtcaaa
     5341 tccgcaggcg tattggtcgg gcgcgtcggc gctatcggac ttgacccgaa atcctatcag
     5401 gcgagggtgc gcctcgattt ggacggcaag tatcagttca gcagcgacgt ttccgcgcaa
     5461 atcctgactt cgggactttt gggcgagcag tacatcgggc tgcagcaggg cggcgacacg
     5521 gaaaaccttg ctgccggcga caccatctcc gtaaccagtt ctgcaatggt tctggaaaac
     5581 cttatcggca aattcatgac gagttttgcc gagaaaaatg ccgacggcgg caatgcggaa
     5641 aaagccgccg aataaacgta tgccatcata ttcaaactag gacacttaat catgaaaaaa
     5701 tcctccttca tcagcgcatt gggcatcggt attttgagca tcggcatggc atttgccgcc
     5761 cctgccgacg cggtaaacca aatccgtcaa aacgccactc aagtattgag catcttaaaa
     5821 agcggtgatg ccaacaccgc ccgccaaaaa gccgaagcct atgcgattcc ctatttcgat
     5881 ttccaacgta tgaccgcatt ggcggtcggc aacccttggc gcaccgcgtc cgacgcgcaa
     5941 aaacaagcgt tggccaaaga atttcaaacc ctgctgatcc gcacctattc cggcacgatg
     6001 ctgaaattaa aaaacgccaa cgtcaacgtc aaagacaatc ccatcgtcaa taaaggcggc
     6061 aaagaaatca tcgtccgcgc cgaagtcggc gtacccgggc aaaaacccgt caacatggac
     6121 ttcactacct accaaagcgg tggtaaatac cgtacctaca acgtcgccat cgaaggcgcg
     6181 agcctggtta ccgtgtaccg caaccaattc ggcgaaatca tcaaagcgaa aggcgtggac
     6241 ggactgattg ccgagttgaa ggctaaaaac ggcagcaagt aatccataag ccatgccgcc
     6301 agactgaaga tggcatggat ttttccctac cgaacactat gcatacagaa ctcaacaacg
     6361 gaacactgca catcagcggc gacgtaaccg tcaaaaccct gaccgccgcc gcctttaccc
     6421 gcttcagaca gcaatgtcgt ctgaaagaca cccgcgcggt tgatttgagc ggcgtcggac
     6481 gcgccgattc cgcctgcgtg tcgctgctgc tcgaagtgct gcgcggttgc aaaggcagcg
     6541 tcaggctgac cggcattccc gaatccgtgc gcgcgctgtc cgaactgtac gaaatcaaag
     6601 actggctgaa atcatgaaaa aaaccgccta tgccttcctc ctgctgatcg ggttcgcttc
     6661 cgcccctgca tttgccgaaa cccgccccgc cgacccttat gaaggctaca accgcgccgt
     6721 tttcaaattc aacgaccaag ccgaccgcta cattttcgcc cctgccgcgc gcggctaccg
     6781 caaagttgcg ccgaaacccg tccgcgccgg cgtgtccaat ttttttaaca acctgtgcga
     6841 cgtggtcagc ttcggtagca atatcttgcg cttggacatc aaacgcgcaa gcgaagacct
     6901 tgtccgcgtc ggcatcaaca ccactttcgg tttgggcggg cttatcgaca tcgccggcgc
     6961 gggcggcatt cccgacaata aaaacacctt gggcgacacg tttgcttcgt ggggatggaa
     7021 aaacagcaat tatttcgtgt tgcccgtctt agggccgtcc accgtccgcg acgcgctcgg
     7081 cacgggtatt acctccgttt attcgcccaa gaatatcgtc ttccgcaccc ctgtcggacg
     7141 ctggggcacg actgccgtat ccgccgtcag tacgcgcgaa ggtctgctcg atttgaccga
     7201 cagtctggac gaagccgcca tcgacaaata cagctacacg cgcgacctct atatgaaagt
     7261 ccgtgcgcgg cagaccggtg caacacctgc cgaaggtacg gaagataaca tcgacatcga
     7321 cgaattggtc gaaagtgccg aaaccggcgc ggcggaaact gccgttcaag aagattccgt
     7381 atccgaaaca caggcagaag cagcagggga agccgaaacg caacctggaa cacaacctta
     7441 aagccatgcc gtctgaaaat ccgtgttcgg acggcattcc cttctgtcgg aacggacagt
     7501 ccgaaaccac agattgggct ataatctctt ttttgcgcat accgcgcgac agcaaaggaa
     7561 aaaccatgta cgaagtcaac cgcagcgtat tcgtcctcat ccctttagaa cctttttgga
     7621 actggctgca aaccctgccg ggcaaccacc tcgacggact gactcttgaa gacatccaag
     7681 ccgacgccaa ttcctacctt gtccgcccgt gcgaaaccgc cgacgaagta tgggacgaaa
     7741 tcgaagcccg ttttgaagac attttcgccg ccgaacttgc cgactggtgc gaagacgaac
     7801 gcgagtggcc tgcgctcgat gccgacattt tcaacgaatg gttcgacatc cagctttcta
     7861 ccgtcatcac cgacctcgaa cacgaaccgc ttgcccgcga agccttccaa cccatcaacc
     7921 tgaactgatg aagctgaccg tccgcaacta ccatctcgac ggctacggcc atgtcaacaa
     7981 tgcgcgctac ctcgaatttt tcgaagaagc gcgctgggcg ttttttgaag aactcggact
     8041 gctgcacgag ttggcaggcc tcatactgat tgtcgcccgc atcgacatcc gatacagccg
     8101 ccccgccgtc gaaggcgacg tattgcagtt ttcatgccgc ctgaaaaccc ccggcacgcg
     8161 ccgtattgtg ctgacccaga cgattacact gccaaacggc aaaaccgccg cagaagccga
     8221 catcaccctg atgcccgtcc acgccgccac acaacgcacc gtcagcctgc ccgccaccct
     8281 tgcccgcgca ttggaagcct tgtccgaatg aaaaaaatac tcaccgccgc cgtcgtcgca
     8341 ctgatcggca tcctccttgc catcgtcctc atccccgaca gcaaaaccgc gcccgccttc
     8401 tccctgtccg acctgcacgg aaaaaccgtt tccaacgccg acctgcaagg caaagttacc
     8461 ctgattaatt tttggtttcc ctcctgtccg ggttgtgtaa gcgaaatgcc caaaatcatt
     8521 aaaacggcaa atgactataa aaacaaaaac ttccaagtcc ttgccgtcgc ccagcccatc
     8581 gatccgatag aaagcgtccg ccaatatgtc aaagactacg gtttgccgtt taccgtcatg
     8641 tatgatgcgg acaaagctgt cggacaggcg ttcggcacac aggtttatcc gacttccgtc
     8701 cttatcggca aaaaaggcga aatcctcaaa acttatgtcg gcgaacccga tttcggcaaa
     8761 ctctaccaag aaatcgatac cgcgctggcg caataaacac atttgccctg ccgtccgaac
     8821 gcaaaggaag ggatgcgggc gcgttcctgc tttttgcatg ttcggcttca gacggcattt
     8881 catcttccgg cggtttttta ccgttgccgg actgcccgaa gccaaagggc gggggatgcc
     8941 ttttgcaggc gcacccgaac cgcgtgccgg tacgatgccc ctccgtccct agactgcctc
     9001 ccggcaaaac ggcggattcc gtaccgcctt taaagcgttt ggctgtcaaa cttgccattg
     9061 ctcgaataaa aaaatattcc ttccgccgtt atctttcccg gcagccggct tcgggcgcat
     9121 tccgtccggg gcggcacttt atgcgccgtc ttgtgccgaa acgccccata aagccccctc
     9181 tggcacggac tttttacatt ttatcgaata tcctcttgac gaaacaacag aaaatagcga
     9241 gaatgccgag cttgtctgaa gcataggtac atcccctatg cccgcttttt tgaaaggaaa
     9301 tcaaatgaaa caaggtattc acccgaacta ccacgaagtc aacgttacct gctcttgcgg
     9361 caacaaattc gcaaccaaat ccgcgatgga aaaagaaaac ttcaatatcg aggtttgctc
     9421 cctgtgccac ccgttctata ccggcaccca aaaaatcgtc gataccaccg gccgcgtgga
     9481 caaattcaac aacaaattcg gcaacctgtt caaacgctga ttgccgcttt gcaagaatta
     9541 agccctgcca tcatttcgat ggcagggctt tttaatattt ggcacactga cttcttat
//
LOCUS       DAXHKX010000063         9492 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHKX010000063 DAXHKX010000000
VERSION     DAXHKX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.063"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..1939
                     /locus_tag="ACFMWZ_001161"
     CDS             <1..1939
                     /locus_tag="ACFMWZ_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC5935129.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAADYIFGDPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRSAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
     gene            complement(2497..3180)
                     /locus_tag="ACFMWZ_001162"
     CDS             complement(2497..3180)
                     /locus_tag="ACFMWZ_001162"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC5935130.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            3477..5780
                     /gene="parC"
                     /locus_tag="ACFMWZ_001163"
     CDS             3477..5780
                     /gene="parC"
                     /locus_tag="ACFMWZ_001163"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC5935131.1"
                     /translation="MNMQPHAPHTDSNTLMLGQYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLSSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGCVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            5801..7377
                     /locus_tag="ACFMWZ_001164"
                     /pseudo
     CDS             5801..7377
                     /locus_tag="ACFMWZ_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            7370..7837
                     /locus_tag="ACFMWZ_001165"
     CDS             7370..7837
                     /locus_tag="ACFMWZ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC5935132.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            7941..8693
                     /locus_tag="ACFMWZ_001166"
     CDS             7941..8693
                     /locus_tag="ACFMWZ_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC5935133.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(9297..9479)
                     /locus_tag="ACFMWZ_001167"
     CDS             complement(9297..9479)
                     /locus_tag="ACFMWZ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935134.1"
                     /translation="MHYKNHLSAVIPTDAKACLFESRIILHRHSRAGGNLVRSVSVIY
                     DKFLLLFISGFPLPWE"
BASE COUNT         2358 a         2911 c         2308 g         1915 t
ORIGIN      
        1 catcagtccc gaaatcttcg gcacgctctt ccaatcagtc atggacgcac aagagcggcg
       61 cgaagcgggc gcacactaca ccgaaaccgc caacatcgac aaagtcatca acggcctttt
      121 tttagaaaac ctgcgcgccg aatttgaagc cgtcaaagcc ctcaaacgcg acaaagacaa
      181 aaaactcgcc gccttctacc aaaaaatcca aaacctgcaa ttcctcgacc cggcctgcgg
      241 ctgcggcaac ttccttatcg tcgcctacga ccgtatccgc gccctcgaag acgacatcat
      301 cgccgaagcg ctcaaagaca aaacaggcgg cttattcgac agcccgtccg tccaatgccg
      361 tctgaaacag tttcacggca tcgaaataga cgaatttgcc gtcctcatcg cccgcaccgc
      421 catgtggctc aaaaaccacc aatgcaacat ccgcacgcaa atccgcttcg acggcgaagt
      481 cgcctgccat acgctgccgc tcgaagacgc cgccgaaatc atccacgcca acagcctccg
      541 cacaccttgg caggcggcgg actacatctt cggcgatccg ccctttatcg gctcgaccta
      601 ccaaaccaaa gagcagaaaa acgacctcga aagcatctgc ggccatatca aaggctacgg
      661 cctgttggat tacgtctgca actggtatgt caaagccgcc ggtatcatgg cgcagcatcc
      721 ccaagttcag acggcatttg tttccaccaa ttccatctgc caaggccagc aggtcgaaat
      781 cctttggggc agccttttga agcaagacat cgaaatccac tttgcccacc gcacctttca
      841 atggacgagc caagccgcag gcaaagccgc cgtccactgc atcatcgtcg gcttccgcca
      901 aaagccgcaa atgccgtctg aaaaaaccct ctacgactat cccgacatca aaggcgaacc
      961 cgtaaaacac gccgcggcca acatcaatcc gtatctgatt gacgcgcccg atgtgattat
     1021 tgccaagcgc agccgtcccg tacattgcga acctgacatg gtcaacggaa gcaaaccgac
     1081 cgaaggcggc aaccttatcc tttcaaccga tgaaaaagaa gccctgattg ccgccgaacc
     1141 cttggcggaa caatacatcc gtccctttgt cggcgcagag gaatttatca acggcaaaac
     1201 ccgttggtgc ctgtggtttc acggcgtatc cgacgttaaa cgcaaccacg acctgaaaca
     1261 gatgccccaa gttcaagccc gtattcaggc agtcaaaacc atgcgcgaag ccagcagcga
     1321 caaacaaact caaaaagatg cagcaacccc gtggcttttt caaaaaatcc gccagccttc
     1381 agacggcaat tttctgatta tcccacgcgt ctcatccgaa agccgccgtt tcgtccccat
     1441 cggttatcta tcatatgaaa cagttgtcag cgatttggta tttacccttc caaacgccag
     1501 cctctaccac ttcggcatcc tcagctccac catgcacaac gcctttatgc gtaccgttgc
     1561 cggccgtctg gaaagtcgtt accgctattc caataccgtt gtgtacaaca acttcccctt
     1621 ccccgaaagc tgccggatgc cgtctgaaaa cgaccgccaa gacccgctcc gctccgccgt
     1681 cgaaaccgcc gcccaagccg tcctcgacgc gcgcggacaa taccgccgag aagcgcggga
     1741 agccggtttg cccgagccga ccctcgccga actctatgcg cccgacgcag gctataccgc
     1801 cctagacaaa gcccacgccg ccctcgacaa ggcagtcgat aaagcctacg gctacaaaac
     1861 aggcaaaaac accgacgacg aagccgaacg caccgccttc ctgttcgagc tgtaccgcaa
     1921 ggcggcggca attgcgtagt agttgcgtag tagttgcgta gtagttgcgt agtagttgcg
     1981 taggatagtt gcgtagggtg ggcttcagcc caccaactcc caccaactcc caccatcttc
     2041 accctaaaac cccgtcatct ccgcaaaagc aattgcgtag ggtgggcttc agcccaccgt
     2101 ttttcttcat tttcggttgt tgattggcag gctgaagccc accctgcggt tacaaaaatc
     2161 ccgtcattcc cgcgcaggcg ggaatccaga cctttcgggg cggaaactta tcggataaaa
     2221 cgatttcccc tgcatcctgg attcccactt tcatgggaat gacgggatat aggtttccct
     2281 acggacgtgt tcagattccc gcctgcgcgg gaatgacggc ggagcggttt ctgcttttcc
     2341 gataaatgac cgcaacctaa aacccatcct tcccacgaaa acagaaaaac aaaaacctaa
     2401 aatcccgtcg ttctcgcgaa agcagttgct acgtttaccg tcaaaaatgc cgtctgaacg
     2461 ctcaagcctt cagacggcat tttttatttc acacccttac aggtagaatt tttcgatgac
     2521 tttcaaattg tcatccaatt tgtacaccag cggctgaccg gtcgggattt ccaagcccat
     2581 aatgtcttcg tcggaaatgc cctcgatgtg ttttgccagc gcgcgcaggg agttgccgtg
     2641 cgccgccacc aagacgcgtt tgccgctcaa aatcgcgggg gcgatttggt cttcccaaaa
     2701 cggcaatacg cgctccagcg ttactttcag gttttcgccg tcgggtacga catcggcagg
     2761 cagatgggca tagcggcggt ctttgtgtgc ggaaaactca tcgtctttgt ccaaaagcgg
     2821 cggcagggtg tcgtagctgc gccgccagat gcggacttgc tcgtcgccgt atttttcggc
     2881 ggtttgtttt ttgtccaggc cttgcagttg gccgtagtgg cgttcgttca gccgccacgt
     2941 tttgatttgc ggtacgaaca gttggtcgga ttcttccaaa acgatgttgc aggtcttaat
     3001 cgcgcgggtc aggacggatg tgaaggcgat gtcgaactca tagccgtttt ctttcagttt
     3061 tttaccggcg gcggcagcct cggcaagccc ctgctcgctc agcttcacgt cgcgccagcc
     3121 tgtaaacagg tttttcgcgt tccattcgct ttgtccgtgg cggataaata ccagttccat
     3181 atcgtctcca atgtgtgaaa gtgggaaagc cttatttata acatattttc acatttcccg
     3241 tatttgattc agattcagac acgcgcccac tatggtttgc cgttttgatt tacaataatg
     3301 ccctttgctt tacattccgc atacacaatg tttcaacaca caggacgaca cataaagcac
     3361 cgccctatgt gttgccctga tttggaaggg gttgccccct cccaaataaa gtctgattct
     3421 accgccccga aggacagatg tccaagtggc gggggttcaa ccgaaaagga aatacgataa
     3481 atatgcaacc gcacgctcca cataccgatt ccaatacgct gatgctcggt caatacgccg
     3541 aacgcgccta tctcgaatac gccatgagcg tggtcaaagg ccgcgcgctg cctgaagttt
     3601 cagacggcca gaagcccgtg cagcggcgca ttttgtttgc catgcgcgat atgggtttga
     3661 cggcgggggc gaagccggtg aaatcggcgc gcgtggtcgg cgagattttg ggtaaatacc
     3721 acccgcacgg cgacagttcc gcctatgagg cgatggtgcg gatggcgcag gattttacct
     3781 tgcgctatcc cttaatcgac ggcatcggca acttcggctc gcgcgacggc gacggggcgg
     3841 cggcgatgcg ttacaccgaa gcgcggctga cgccgattgc ggaattgctg tcgtccgaaa
     3901 tcaatcaggg gacggtggat tttgtgccga actacgacgg cgcgtttgac gaaccgctgc
     3961 acctgcccgc ccgcctgcct atggtgttgc tcaacggcgc gtcaggcatt gcggtgggca
     4021 tggcgaccga gattccgtcg cacaatttga acgaagtgac gcaggcggcg attgcgttgt
     4081 tgaaaaagcc gacgctggaa accgccgacc tgatgcaata cattcccgca cctgattttg
     4141 ccggcggcgg tcaaatcatc acgccggcgg acgaattgcg ccggatttat gaaaccggca
     4201 agggctgcgt gcgcgtgcgt gcgcgttatg aaatcgaaaa attggcgcgc ggacaatggc
     4261 gcgtcatcgt aaccgagcta ccgccgaacg ccaattccgc caaaatcctt gccgaaatcg
     4321 aagagcagac caacccgaaa ccgaaagcgg gcaaaaagca gctcaaccaa gaccagctca
     4381 ataccaaaaa gctgatgctg gatttaatcg accgcgtgcg cgacgagtcc gacggcgaac
     4441 atcccgtgcg actggtgttc gaaccgaaat ccagccgcat cgataccgat accttcatca
     4501 acacgctgat ggcgcaaact tcgctggaag gcaatgtgtc catgaacttg gtgatgatgg
     4561 ggctggacaa ccgccccgcg cagaaaaacc tgaaaacgat tttgcaggaa tggctggatt
     4621 tccgcaccgt aaccgtaaca cgccgtctga aattccgttt gaaccaagtg gaaaaacggc
     4681 tgcacatcct cgaaggccgt ctgaaagtct ttctgcacat cgacgaagtg attaaagtca
     4741 tccgcgaatc agatgacccg aaagccgatt tgatggcggc gttcgggctg accgaaatcc
     4801 aagccgaaga cattttggaa atccgcctgc gccagttggc acgtttggaa ggtttcaaac
     4861 tcgaaaaaga attgaacgaa ttgcgcgaag aacaaggccg tctgaacatc cttttgggcg
     4921 atgaaaacga aaagcgcaag ctgattatca aagagatgca ggcggatatg aaacaatacg
     4981 gcgatgcacg ccgcacgctg gtggaagagg ccggacgcgc cgtgctgaca caaaccaccg
     5041 ccgacgaacc catcacactg attctgtcgg aaaaaggctg gatacgcagc cgtgccggac
     5101 acaatctcga tttgagccaa accgcgttca aagaaggcga ccgcctcaaa caaacccttg
     5161 aaggcagaac ggttttaccc gtcgtcatcc tcgattcatt gggcagaacc tatacactcg
     5221 atgccgccga aattcccggc ggacgcggcg acggcgtgcc ggtttcatcc ttgatcgagt
     5281 tgcaaaacgg cgcgaaaccc gttgcgatgt tgacaggatt gccggaacaa cattatttat
     5341 tatcaagcag cggcggctac ggcttcatcg ccaagttggc tgatatggtc ggacgcgtaa
     5401 aagcgggcaa agtggtgatg accacagaca gtggcgaaac cgtcctgccg ccgattgccg
     5461 tctatgcctc ctcgttcatc aaccccgact gcaaaatcat tgccgccacc agtcaaaacc
     5521 gcgccctcgc cttccccatc ggcgaattga aaattatggc gaaaggcaaa gggctgcaaa
     5581 tcatcggatt aaacgccggc gaatcgctga cgcataccgc cgtttcttcc gagccggaaa
     5641 tcctgattga aagcgaaggc aggcgcggcg cggcgcacaa agaccgcatc cccgtctccc
     5701 tgcttgaggc aaaacgcggc aaaaaaggca aactattacc catatcgggc agcctgaaac
     5761 agctttcttc ccccaaataa acccgattcc acacataatt atggtgattt ccaacccccg
     5821 cgaacttgaa aaactcaaag accggattcc caatctgatc aacatcatcc gcgtcgccat
     5881 cgtttttccg ctgatgatta tgcacatcct cgggctggaa accggcagcc gtgcgaacct
     5941 gcacgcttcg tggacggcgt gggcgtttta tgtttggctc gccattgcct gctggctgat
     6001 tttcttttcc atcatccatc cgcattggca atggcaggct ttgagaatac cgagtttcag
     6061 cgcggtggcg gacatcacga tgatcggcgt gctgacctac ctgttcggcg gcatcgactc
     6121 cggcttcggc atcctgattc tgcccttcgt cgtctgctcc tgcctgctca gctacgggcg
     6181 ctaccccctg ctctattcca gctacgccgc catcctgctg atattcaacg ccattgccga
     6241 cggcgatatc ggcaaatacc cgctcatatc ggatgcccga accgcctcgg caaccttcat
     6301 ccttgtcgcc gcctcctatc tttccgccat cttcacctcg ctgtcggtca gatacatcga
     6361 ccgtgccggc aaacttgccc atgaaaacca cgccgcctac cgccgcatca ggggcttgaa
     6421 ccaaaccgta ctcaaccgcg ttcaggaagc ggtcgtcgtc atcaacgtcg agcatcagac
     6481 catactgttc aataaaaagg caaaagacct gctacccatg cttgaaatcg gacagcatac
     6541 cgccctgttc gaccctatta ccgttttatg ggacaaggcc tcttcgcgta ctttcgaacg
     6601 caatatcgac acgcccggcc tgaacgccgg catccgcgcc gttccggtca acaaagggcc
     6661 ggacaagctg ctcatcctct acatccgccc gcaaagcgaa attcaggcag aagccctgtc
     6721 cgtcaaactt gccgcgctcg gacaactgac cgccaacctc gcccacgaaa tccgcaaccc
     6781 gatgtccgcc atccgccacg ccaacgacct gctgcgcgaa aatatggaag cgggggcggc
     6841 agatccgttc aacgccaaat tgtgcaaaat catcgacggc aacgtctgcc gcatcgacaa
     6901 aatgctcgaa gatatttcct cgctcaacaa gcgcaacaaa accgaacgcg aaaccatcgg
     6961 cctgataccg ttttgggaag aattcaaaca agagttcctg ctcggccatc ccgatgccgc
     7021 cggctgcatc cgcctagata tgcagggcaa tcacctgact gcctatttcg atcctgccca
     7081 cctgcggcaa attatgtgga acctcgccaa caacgcgtgg cggcacagcc gcaaacagcc
     7141 cggctcgatt tccgtcacca tccgccccgc gcaaaaaaac accgtctgca tcctctttgc
     7201 cgaccgcccg aagtgcagga acacctgttc gaaccctttt acaccacggc ggaaaacggc
     7261 accggcctcg ggctgtatgt cgcccgcgaa ctggcgcacg ccaatttcgg cgatttgacc
     7321 tacctaccgg aagccaaatg tttcgaactc acattaccgg aaaaaaccaa tgactgaact
     7381 gcaacacccc gtcctcgtcg tcgatgacga aaccgacatt ctcgacctga tggaaatgac
     7441 cctgatgaaa atgggcttgc gcgtccatac cgcgtcaggc gttgccgaag ccaaaaacaa
     7501 gctcgacagc caacgctatt cgctcgtcct gaccgatatg cgtatgccgg acggctcggg
     7561 gctggaagtc gtccaacaca tcaacagccg cctgctcgat acgccggttg ccgtcatcac
     7621 cgccttcggc aacgccgatc aggcacagga agcgttgcgt tgcggcgcgt tcgaccccga
     7681 taccatgcag atacaggact atctcgacca aatcgaacgc gacattatcg aacaaaccct
     7741 caaacaaacc gaaggcaacc gcacgcaggc cgccaaacgc ttgggcatca gcttccgttc
     7801 catgcgctac cgtatggaac gcctcaacat cggctgacga caaaacggca tccgcaccat
     7861 ctccgcccac ccgaaaaaat gccgtctgaa acggcacggg aaagcgggtt cgccccacgc
     7921 ccgaacggac acaaaacacc atgaccgaca tccttattga caacaccgcc accgaaaccg
     7981 tccgcaccct gatacgggca ttcccccttg tgcccgtttc ccaaccgccc gaacaaggca
     8041 gttacctcct tgccgaacac gataccgtca gcctcaggct tgtcggggaa aaaagcagcg
     8101 tcatcgtcga ttttgcctcc ggcgcggcac aataccggcg cacaaaaggc gggggcgaac
     8161 tcatcgccaa agccgtcaac cacaccgcgc accccaccgt ttgggacgca accgcaggat
     8221 tggggcgcga cagcttcgtc ctcgcctcgc tcgggctggc cgttaccgcc ttcgagcaac
     8281 atcccgccgt cgcctgcctg ctttcagacg gcatccgccg cgccctcctc aatcccgaaa
     8341 cgcaagacac cgccgcgcgc atcaccctgc atttcggcaa cgccgcagta caaatgcccg
     8401 cacttgttca aacacaaggc aaacccgaca tcgtctatct cgaccccatg tatcccgaac
     8461 gccgcaaaag tgccgccgtc aaaaaagaaa tgacctactt ccaccggctc gtcggtgaag
     8521 cgcaagatga ggccgccctc ctccataccg cacgccaaac ggcaaaaaaa cgcgtcgtcg
     8581 tcaaacgccc ccgcctcggc gaacaccttg ccggacaagc ccctgcctac caatacaccg
     8641 gcaaaagcac ccgcttcgac gtttacctgc cctacgggac ggacaaggga taacgcccat
     8701 aaaacaagac accgaaaaat ttgccgttct tatgcaaacg agaaaccggt ttttgcgttt
     8761 cgactgtttt ggataaatca tcacacctta aagtttgtca ttcccacgga agtgggaatc
     8821 cagaaatgaa aagcaacagg aatttatcgt aaatgaccga aaccgaacgg actagattcc
     8881 cgcctgcgcg ggaatgacgg ggcgggcgga tgccgtctga aattccgtca ttcccgtaaa
     8941 aacgggaatc tagaacttct gatttttcag acgacttttg aacattgccg ctacccaatg
     9001 atctggattc ccgcctgcgc gggaatgacg aggtttcagg ttgctgtttc gggttgctgt
     9061 tttttatgga aatgacgagg ttttagattg cgagaattta tccgctccgc cgtcattccc
     9121 acggaagtgg gaatcaggaa atgaaaagca ataggaattt atcgtaaatg accgaaaccg
     9181 aacggactag attcccgcct gcgcgggaat gacgaggttt caggttgctg tttcgggttg
     9241 ctgtttttta tggaaatgac aaggttttag attgcgagaa tttatccgct cctccgtcat
     9301 tcccacggaa gtgggaatcc agaaatgaaa agcaacagga atttatcgta aatgactgaa
     9361 accgaacgga ctagattccc gcctgcgcgg gaatgacgat gtaaaattat ccgggattca
     9421 aaaagacagg ctttcgcatc cgtgggaatg actgcggaaa gatgattttt atagtggatt
     9481 aaatttaaat ca
//
LOCUS       DAXHKX010000064         9417 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHKX010000064 DAXHKX010000000
VERSION     DAXHKX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.064"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            9..2264
                     /locus_tag="ACFMWZ_001168"
                     /pseudo
     CDS             9..2264
                     /locus_tag="ACFMWZ_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
     gene            2443..2976
                     /locus_tag="ACFMWZ_001169"
     CDS             2443..2976
                     /locus_tag="ACFMWZ_001169"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC5935135.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(3039..4226)
                     /locus_tag="ACFMWZ_001170"
     CDS             complement(3039..4226)
                     /locus_tag="ACFMWZ_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC5935136.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTAPASNNAPSAPSHPVA
                     KARTVYPKPPRHSNSADNLLRIVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRR
                     FQRFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHG
                     TLFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTV
                     DVPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLN
                     TLYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHI
                     ADKITFPLGAHARIRMNGNGGYAVAFGGYPTLDASALTLDTLLPPPDLPFFPESGVAD
                     ISE"
     gene            complement(4266..4670)
                     /locus_tag="ACFMWZ_001171"
     CDS             complement(4266..4670)
                     /locus_tag="ACFMWZ_001171"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC5935137.1"
                     /translation="MNTKPCPICTAQDEDILLQTPNLRVIAIHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            complement(4667..5020)
                     /locus_tag="ACFMWZ_001172"
     CDS             complement(4667..5020)
                     /locus_tag="ACFMWZ_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC5935138.1"
                     /translation="MVQLLATEADRLIGEKIRQRRKELGYSAEKLAEHINLSQQQISR
                     YERGVGKINISHLVDIAVFLETPIAWFFQDCLPKPLTVACTPENTPEQQWHSLNPNQK
                     AAFAAFLDTLRNKET"
     gene            5172..5585
                     /locus_tag="ACFMWZ_001173"
     CDS             5172..5585
                     /locus_tag="ACFMWZ_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935139.1"
                     /translation="MNKFLATWLAPILVLSGCATIMGESSQTVPIKSQPSGAVFKIQD
                     ETGVIVQSGVTPANVSLPKGNGKYFGRKKYQVSFEKKGYQPLVYKLETNPNGWYIGGN
                     LIFGGLIGYLIVDPLSNGKMFTISPKQVDVPLIAK"
     gene            complement(5656..6093)
                     /gene="rnhA"
                     /locus_tag="ACFMWZ_001174"
     CDS             complement(5656..6093)
                     /gene="rnhA"
                     /locus_tag="ACFMWZ_001174"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC5935140.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRHCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENEHADDLANRGAAQFS"
     gene            6207..7076
                     /gene="tehB"
                     /locus_tag="ACFMWZ_001175"
     CDS             6207..7076
                     /gene="tehB"
                     /locus_tag="ACFMWZ_001175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC5935141.1"
                     /translation="MKEWIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(7126..7932)
                     /gene="thiD"
                     /locus_tag="ACFMWZ_001176"
     CDS             complement(7126..7932)
                     /gene="thiD"
                     /locus_tag="ACFMWZ_001176"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC5935142.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            7993..8190
                     /locus_tag="ACFMWZ_001177"
     CDS             7993..8190
                     /locus_tag="ACFMWZ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935143.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(9234..9309)
                     /locus_tag="ACFMWZ_001178"
     tRNA            complement(9234..9309)
                     /locus_tag="ACFMWZ_001178"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9273..9275),aa:Glu,seq:ttc)
BASE COUNT         2122 a         2257 c         2632 g         2406 t
ORIGIN      
        1 ctctcaaaat gggacagtac aagaagctgt ggtacttgct gtttgccgtt ctggcggtat
       61 gctttaccat tcttggctat atgggcagcg aggtttataa gaaagccccg ccttaccccg
      121 aacaggtcgt ttccgcatca ggcaaagtgc tgatgacgaa agacgatatt ttggcaggtc
      181 agtctgcgtg gcagagtacc ggcggcatgg aagtcggttc gattctgggt cacggcgcat
      241 atcaggctcc ggactggacg gccgactggc tgcatcgtga gttgtccgcc tggttggatt
      301 tgaccgcgca acagacttac ggcaaaaaat tcgatgaagt ttcccctgaa gaacaagccg
      361 ttctgaaaac ccgactggct gacgaatacc gcaaccaaag ccgtattaaa gaagatggtt
      421 ctgtggtcat cagcgatacg cgtgtaaaag ccatcgaaag catcctgcct tattatcacg
      481 gtgtttacgg cgatgatccc gcgctgcaaa ccacccgcga acactttgca atgaaaaaca
      541 acacattgcc aagccaagaa gcgcgtgaaa aactgttcga ctttttcttc tggacttctt
      601 ggtctgcttc gaccaaccgt cctgacgaga cgtttaccta taccaacaac tgaccgcacg
      661 agcctttgat caacaatgtg ccgaccactg aaaactatat gtggtcgttt accagtgtcg
      721 tactgctctt gatgggcatc ggtttgctga tgtggggtta ttccttcttg accaaacacg
      781 aggaagtgga agtgccgtct gaagacccga tttccaaaat ccaactgacc ccttcgcaaa
      841 aagcattggg caaatacgtc ttcctgacgg tcgccctgtt tgtggtacaa gtattgctgg
      901 gcggtctgac cgcgcactac accgtcgaag gtcagggttt ctacggcatt gacgaggcgt
      961 tgggctttga aatgtccgac tggttccctt acgccctgac ccgtacttgg cacatccaat
     1021 ccgccatctt ctggattgca accggctttt tgacggcagg tttgttcctt gcgccgattg
     1081 tcaacggcgg caaagatccc aagttccaac gtgcaggcgt gaacttcctc tatatcgccc
     1141 tcttcatcgt ggtcggcggc tcttacgcgg gcaacttctt tgcgttgacg cacatccttc
     1201 cccccgaatt gaatttctgg ttcggacacc aaggttacga atacctcgat ttgggacgtt
     1261 tctggcaact cctgctgatg gtcggcctgc tgttgtggct gttcctgatg ttgcgctgca
     1321 cggtttccgc cttcaaagaa aaaggcgtag acaaaaacct gctggcaatc tttgtcgctt
     1381 ctatggtcgg tgtgggcgta ttctacgcac cgggcctgtt ctatggcgaa aaatccccga
     1441 ttgccgtaat ggaatactgg cgctggtggg tggttcacct gtgggtggaa ggcttctttg
     1501 aagtatttgc cactgccgcc tttgcctttg tcttctacaa tatgggcttt gtccgccgca
     1561 gtaccgccac tgcctccact ttggccgctg ccgccatctt tatgttgggc ggcgttccgg
     1621 gtacgctgca ccatctgtac ttctccggct ctacctccgc ctctatggca atcggtgcct
     1681 gcttctccgc tttggaagtc gtgccgctgg tgttgctggg ccgtgaagca tacgaacact
     1741 ggtcttacca acacctgtcc gactgggcga aacgcctgcg ttggccgctg atgtgcttcg
     1801 tggcagtcgc cttttggaat atgatcggtg ccggcgtatt cggcttcctg atcaatccgc
     1861 cgatttccct gttctatatc caaggtttga acacttctgc cgttcacgcg cacgccgcct
     1921 tgtttggtgt gtacggtttc ttggcattgg gcttcgtatt gctggttgcc cgttatttga
     1981 aaccgaacgc gcagtttgac gacaaactga tgacttgggg cttctggctg ctcaacggcg
     2041 gcttggtcgg tatgattgcc atcagcctgc tgcctgtcgg cgcgattcag gcatacgcct
     2101 ctatcacgca cggcctgtgg tatgcacgca gcgaagagtt cctgcaaatg gaaatcctcg
     2161 acaccctgcg ctgggttcgt actgccgccg acttgatctt tatcggcggt gcaatctgtg
     2221 tcgccatcca agccactaaa atcgtattcg gccgcgacaa ataagttttg cggtatgaaa
     2281 tgagcaactg cacccgtttt cgggtgcagt tttttattgc ggattgggac gggaatggaa
     2341 aaggatgtgg attttttatt ttaatcggct atacaattgc tgctttcatg ccgtctgaag
     2401 cacgtttcag atggcatcgg tttacatcag ataaggataa aaatgggtat ttacgatttt
     2461 caaatgaaag atgcagaagg caatgcggtt gatttgtcag gctatcgcgg caaggttctg
     2521 ctgattgtca acacggcaac gcgttgcggt ctgaccccgc aatacgaggc tttgcagaag
     2581 ctgtatgcac aatataccgc agaaggcttg gagattttgg attttccgtg caaccagttc
     2641 cgcgaacagg ctcccgaaag cagcggagaa attgcccaag tgtgtatgat gaagtttggc
     2701 acgaagttta agattttcga caaaatcgaa gtcaatggag caaataccgc gcccttgtat
     2761 gcctacctga aatccgtcaa accgcaagat aaaggcaatc atctgtttaa agatttcgtg
     2821 ttgaagctcg cagctttggg agagaagcgc gacgaaggcg acatcaaatg gaactttacc
     2881 aaattcctcg tcaaccgcga cggcgaagtg gtcgagcgtt ttgcgcctag tgttacgccc
     2941 gaagaaatcg aagccgatat tcgggcattg ctgtaatctg ccgccgtctt gggaaatgcc
     3001 gtctgaaggc ttcagacggc atttgtccgt ttgcgggttt attccgagat atcggcaaca
     3061 ccgctttcgg ggaagaaggg caaatccggc ggtgggagca gggtatccaa agtcagggcg
     3121 gacgcatcga gtgtcgggta gcctccaaac gcgaccgcat aaccgccgtt tccattcata
     3181 cggattcggg cgtgcgcgcc tagagggaaa gtgattttgt cggcaatgtg tccgaacggg
     3241 aagcccgtca gcacggggat tttcgccgtg cgcgaaacat ggttggcaac ggcagaaaaa
     3301 tcataagacg gatcatagac atctcgaatt ttttccatac ggaaattgcc gaacacgatg
     3361 gcgcgctgtt tcttcaaaat acccgaaaga tacagcgtat tgagcatacg ttcgatgcgg
     3421 tagggctgtt cgccgacatc ttcgaggaac aaaatgccgc cgtcgatgtc gggcatataa
     3481 ggcgtgccgg cgagcgaggc gaggacgctt aagttgccgc cccacaatat gccttcggtt
     3541 tcgacatcgg cgcgttggat ataaggaaca tcaacggtca ggcggttttg ggttgcaccc
     3601 ttgataaagg catccatcgt aaacgcaccg ggggcgggtt tgccaaaatc gctataagcc
     3661 atcgggccgg caaaactcat catattgcct tttgccaaca atgccagctg gacggcgcat
     3721 acgtcgctga atccgaaaaa gagcgtgccg tgttcgcgca tccttgcgcc gagcgaagca
     3781 aaatcgatat gcggcagaat ccgcgccgca ccgtaaccgc cgcgcaagcc catcagcact
     3841 ttaggcgtgg cgacgcggcc ggaagcgacc tcttggaaat cggcggcacg ttgtgcgtcc
     3901 gtaccggcaa accgttggaa acggcggctg cccgcctgtt ggttggttac ggtaaaaccg
     3961 gcattgtaaa ggcgggttaa ggctgtgttg acgcggttgg tgtcttcggc aaatcctgac
     4021 gaagcgacaa tacgcagaag attgtcggca gagttggaat ggcgtggtgg tttggggtac
     4081 acggttcggg ctttcgcaac aggatgagag ggcgcggatg gggcattgtt tgaagcaggc
     4141 gcagtaccgc aagcctgaag cagccccgcg ccggcggcgg cggtgcaggt tttcaggaaa
     4201 cggcggcggg aggtcggttc ggtcataatt tccctttcat acggtttcag acggcattgg
     4261 cgggcttaaa gcagcttttt aagctgttcc gtccaatctt gcggcagggt cataccgtgt
     4321 tttcggacgg ggtttgccca aatcggcgcg gggaaagacg catcgttttc aaagcgggcg
     4381 ataatatgcc agtgcaggtg cggcacgaca ttgcccaagc tggcgaggtt gatttttgcc
     4441 ggccggaaca cttggcgcat agcggcttcg actttgtaca ccatttccat caattcgccg
     4501 cgttccgctg ccgaaaggtc ggtcatttcg gcaatatgct tacgccaaat gacgcggcag
     4561 aatgcaggcg aaccgctgtc gttatggatg gcgatgacgc ggaggttggg ggtttgcagc
     4621 aaaatatctt catcttgtgc cgtgcagatg gggcagggct tggtgttcat gtttctttat
     4681 tccttaaagt atccaaaaag gcggcaaagg cagctttttg gtttggatta aggctgtgcc
     4741 attgctgttc gggtgtgttc tcaggagtac aggctaccgt cagtggtttg ggcaggcaat
     4801 cttggaaaaa ccaagcaatc ggtgtttcca gaaaaacggc aatatcgact aagtgcgaaa
     4861 tattaatttt acctacgcca cgttcgtagc gtgaaatctg ctgttgagaa agattgatgt
     4921 gttccgccag cttttctgca gaatagccca actcttttcg tcgttgtcgg attttttcac
     4981 ctattagccg gtctgcttct gttgcaagca attgtaccat tttgtatccg attgtagatt
     5041 tactcatcta tggatattta tcttgttttt tctcttgtaa acagtgttgt aaataagtaa
     5101 aatattccaa attacatcat tattgtgtaa tttaatgtat tttatttttt agtcagttgg
     5161 agatgatttt gatgaataag ttcttggcaa catggcttgc accgattttg gttttgtcgg
     5221 gttgtgcaac gattatggga gagtccagtc agaccgtgcc gattaaaagt caaccatccg
     5281 gtgcggtatt taaaattcag gatgaaacag gtgtgattgt tcaatcaggt gtaacgcccg
     5341 caaatgtttc gttgcctaaa ggcaacggca aatatttcgg caggaaaaaa tatcaggttt
     5401 cttttgagaa gaagggttat cagccgttgg tttacaagtt agaaaccaat ccgaatggtt
     5461 ggtatattgg tggaaacctg attttcggtg gcctgattgg ttatctgatt gttgatccgt
     5521 tgagtaacgg taagatgttt acgatttcac ccaagcaggt agatgtaccg ttaattgcga
     5581 aataaggagt gtatggtaaa aatgccgtct gaagcccatt agcggtttca gacggcattt
     5641 ttgccggagc ggcagtcagg aaaactgcgc cgcgccacgg tttgccaaat cgtcggcgtg
     5701 ttcgttttcg gcgtgtcccg cgtgtccttt cacccaagtc cagctgactt gatgccgtcc
     5761 gaccagagcg tcgagttctt tccataagtc gtcgtttttg acgggctgtt tggcggcggt
     5821 tttccagccg ttgcgcttcc aaccgtgtat ccagttttcc atgccgtttt taacgtattg
     5881 cgagtcggtg cagatgatga cggtgcagtg gcgtttgagg gatttcagtc cttcgatgac
     5941 ggcagtcagt tccatgcggt tgttggtggt ttgcgcttcg ccgccgaaaa gttctttttc
     6001 gtggctaccg tagcgcatta acacgcccca gccacccgcg ccgggattgc ctttgcacgc
     6061 gccgtcggtg taaaggtaaa cggtttggtc cattattgtg cctttgtgtg gtgtccggcg
     6121 tgattataag cggtttgtgc gccgtgccgt ctgaaagatt gcggcggatt aaaataggca
     6181 tataatgtgc aactttattt ttgaatatga aggagtggat agtgggtcaa agtggcgagt
     6241 tgttttgttt cggacaaatg cctgtatgga aagtggaaaa cctgccggaa gttttgttgt
     6301 cgggctattc gtctgaggaa ggggagtggg tctgcctgaa tgtgttgcag ggcgatgtcg
     6361 aagtccgcac gcaggacggg gcggcggagg tttggtcggc agaaagcggc gattgcgtgt
     6421 ttgcgccgca gcaggtgttt tcggtcaaac cgaaaacgga cgatgccgaa atccgtttgt
     6481 cgctgtattg cgcggcggca gactattttc acaaaaaata cgggatgagt gccacgcatt
     6541 ctgcggtcgc ggcggcacag gataccgtac cggcgggcag ggcgttggat atgggctgcg
     6601 gacaggggcg caacgcgctg tttctcggtt tgaaggggtt tgaagttact gcagtcgatc
     6661 acaatcccgc cgccttggca aacgtggcgg agctggcaga ggcggagggt ttgaacgtcc
     6721 gcacgctgga atatgatttg aacgccgccg ccttgcaggg tgagtttgat tatattgtgg
     6781 caacagtggt gctgatgttc ctgatgccgc agcgcgtgcc tgacgtgatt gccgatatgc
     6841 aggcgcatac ggcggcgggc gggtacaact taatcgtatc ggcaatggat acggcggatt
     6901 tcccttgtcc gatgccgttc ccttttaaat ttaaagaggg agagctgaag gattattatc
     6961 gggattggga gctggtcgaa tataaagaag aattgggcgc gatgcacgcc aaagacgaaa
     7021 acggcaatcc gatacggttt aaatttgtaa cgatgctggc gaaaaagcct gaataatcct
     7081 caaaaatgcc gtctgaacat tttgtttcag acggcatttt tacggttagt cccgataggc
     7141 ccaatgattg accgggccgt gtcccgcgcc gatttccaaa gggcttgaga ttgccgccgt
     7201 gatgtaggac ttggcagtcc gtacggcttt gcaaacgtct aagccttttg ccaactcggc
     7261 ggtaatgcag gcagaaaacg tgcagcccgt gccgtgcgtg tgggcggtcg gaaagcgcgg
     7321 gctgtccaat tccagcgttt cgttttgcgt aaacagccaa tccgtgcagc gtccgctgct
     7381 gctgccattc aaatgtccgc ctttgatgac gacatttttg acaccgtaat caagcaggat
     7441 ttttgccgca cgttccgcat ctttacggtt ttcgatatgc acgccggtca gggcttccgc
     7501 ctcgggcagg ttgggggtca atacatccgt atcgggaagc agcaggcgcg tcagtgccgc
     7561 aacggcggaa tcctgcaaca gcggcgcacc gcctttggca atcatcacag ggtcgagtac
     7621 gcgcctgccg aagtcgcagt gtttcagctt gtcggcaacg cattcgatga tttccgccgt
     7681 gccgagcatt cctattttgt aggcgcggat gtcaaagtct tccctgattg cttggatttg
     7741 tgcggtgatg gtttcggtcg ggacgagatg aaccgccgac acgcccaagg tattttgcgc
     7801 ggtaacggcg gtgatgacgc acgttccgaa cacgccgcgc atctgaaacg ttttcaaatc
     7861 cgcctgaatg cccgcaccgc cgcccgaatc cgaaccggcg atggtaagcg tttgcacaaa
     7921 agagcttttc atttgttcct ccttaatatt gtgtccgata aaaagggcta atgcctgtaa
     7981 aaaagcacgg cgatgccgtc tgaaaaccgc agcgcggggg tttgtggtgc attatatgcc
     8041 gtattgcagg acaaggcaca gggcagggcg gcggggtgca tcgtagcaac tgtatttttg
     8101 cccgacgggg tgaaaaatac agttgctaca cccactgata atgaaaaaag agagcaatgc
     8161 cgtctgaacc gcgtgttgcg gcgagcataa accggcacgc tttattcgtc atttccacaa
     8221 aaacagaaac ctaaaattcc gtcattccta cgaaagcggg aatccagtgt gttcggtttc
     8281 agttgttttt gggtttcggg tgatttccaa atcatcattc ccgcgcaggc gggaatctag
     8341 cccttggggc agcggcaata tttataaagc cgtctgaaaa ttcagaagtt ctagattccc
     8401 gttttcacgg gaatgacgaa gggttgtggg aatgacgaag tttcagacgg catcgccccg
     8461 ccccgtcatt cccgcataag cgggaatcca aaccattggg cagagcagcc ttaaaggtta
     8521 taaaagactc gtcattccca cgaaagtggg aatccagacc tgtcggcacg gaaacttatc
     8581 ggataaaacg gtttcttcaa ccctacgctc tagattcccg cttttgcggg aatgacgaag
     8641 tttcagacgg catcaaatgc tttgtcaggc ttcagacagc atcgtccacc ctctgccgtc
     8701 cttcccgcgc aggcgggaat ccaatgtgtt cggtttcggt tgtttttggg tttcgggtaa
     8761 tttccaaatc gtcattccca cgaaagtggg aatccagacc attgggcggc ggaaatattc
     8821 aaaagtcgtc tgaaaattca gaggttctag attcccgttt tcacgggaat gacgaagggt
     8881 tgtgggaatg acgaagtttt agacggcatt gaatactttg atgctttggt gggctgaagc
     8941 ccaccctaca atatagagaa aatgccgcca aacggtatgg aagcggaaac aatggtgggg
     9001 ctggcttcag aaacaatggt agggtgggct tcagcccacc gctttagacg gcatccgcgc
     9061 accgtcattc ccgcgcaggc gggaatccag ccttgtcggc acggaaactt atcggataaa
     9121 acggtttctt caaccctacg ctctagattc ccgttttcac gggaatgacg aagagtgtga
     9181 gaatgacggt tcataaataa aaaaacctgc ttaaaacaag caggtttttg aattggcacg
     9241 cccacgggga ttcgaacccc ggttgccgcc gtgaaagggc agtgtcctag gcctctagac
     9301 gatgggcgcg tgggaatggg cattatacat acttctagaa atttagcaat acgatgccgt
     9361 ctgaaacttc gtcattcccg cgaaagcggg aatctagaat ctccaagttt tcagaca
//
LOCUS       DAXHKX010000065         9396 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHKX010000065 DAXHKX010000000
VERSION     DAXHKX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.065"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            190..1008
                     /gene="opc"
                     /locus_tag="ACFMWZ_001179"
                     /gene_synonym="opcA"
     CDS             190..1008
                     /gene="opc"
                     /locus_tag="ACFMWZ_001179"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC5935144.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTTSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1171..1815
                     /locus_tag="ACFMWZ_001180"
     CDS             1171..1815
                     /locus_tag="ACFMWZ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC5935145.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            1834..3744
                     /locus_tag="ACFMWZ_001181"
     CDS             1834..3744
                     /locus_tag="ACFMWZ_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC5935146.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHDLEHNSDDVLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            3765..4130
                     /locus_tag="ACFMWZ_001182"
     CDS             3765..4130
                     /locus_tag="ACFMWZ_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935147.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4241..5716
                     /gene="trpE"
                     /locus_tag="ACFMWZ_001183"
     CDS             4241..5716
                     /gene="trpE"
                     /locus_tag="ACFMWZ_001183"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC5935148.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVVDNLSGKIYLIVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSSLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            5713..6258
                     /locus_tag="ACFMWZ_001184"
     CDS             5713..6258
                     /locus_tag="ACFMWZ_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFC5935149.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            6255..7391
                     /locus_tag="ACFMWZ_001185"
     CDS             6255..7391
                     /locus_tag="ACFMWZ_001185"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC5935150.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7438..7920
                     /locus_tag="ACFMWZ_001186"
     CDS             7438..7920
                     /locus_tag="ACFMWZ_001186"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC5935151.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8026..9081
                     /locus_tag="ACFMWZ_001187"
     CDS             8026..9081
                     /locus_tag="ACFMWZ_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC5935152.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9078..9290
                     /locus_tag="ACFMWZ_001188"
     CDS             9078..9290
                     /locus_tag="ACFMWZ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935153.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGALQGRITFLNHPF"
BASE COUNT         2622 a         2664 c         2056 g         2054 t
ORIGIN      
        1 tcgtcattcc cataaaaaac agaaaaccaa gtgagaataa caattcgttg taaacaaata
       61 actatttgtt aatttttatt aatatatgta aacccccccc gaaagcttaa gaatataatt
      121 gtaagcgtaa cgattattta cgttatgtta ccatatccga ctacaatcca aattttggag
      181 attttaacta tgaaaaaaac agtttttaca tgtgccatga ttgccctgac cggtactgcc
      241 gccgctgcac aagagcttca aaccgctaat gagtttaccg tccacaccga cctctcttcc
      301 atttcttcaa ctcgtgcttt cctgaaagaa aaacacaaag ctgccaaaca catcggcgta
      361 cgtgctgata ttccttttga tgccaaccaa ggcatccgct tggaagccgg tttcgggcgc
      421 agcaaaaaaa atattattaa tttggaaaca gatgagaaca agctgggtaa gactaaaaat
      481 gtaaaactgc ccaccggcgt tcctgaaaac cgtatcgatc tttacacagg ctacacctat
      541 acccaaacgt taagtgattc tttaaatttc cgtgtgggtg ccggcttggg ttttgaatct
      601 tcaaaagaca gcattaaaac caccaagcat acgcttcaca gcagccgtca gtcgtggtta
      661 gccaaagttc acgcggattt gctttcccaa ctgggtaacg gctggtatat caacccttgg
      721 tctgaagtga aatttgacct caattcccgc tataaattaa acaccggcgt taccagtctc
      781 aaaaaagaca tcaatcaaaa aaccaacggc tggggctttg gattgggtgc aaatattggt
      841 aaaaaactgg gcgaatccgc cagcatcgag gcggggccgt tctacaaaca acgcacttac
      901 aaagaatccg gcgagtttag tgtaacaacc acgagtggcg acgtatcgct caccatcccg
      961 aaaaccagta ttcgtgaata cggcttgcgc gtcggcataa aattctgatg atttgaaatc
     1021 atcattgtca ctttaaatgc caaaccgcaa actattttgg tttgcggttt ttacgtgaaa
     1081 tgaatttgaa tagccgatgc cgtctgaaaa tttcagacgg catttttcca atcggttaaa
     1141 atacgcggtt tgttttttta ggaacgcgcc gtgcttgcct ccgccatcga cttcatcctc
     1201 catatcgacc aacacctgct cgcgctgtcg gcgcaatacg gtgtgtggat ttatgcgatt
     1261 ctgtttttga ttgttttttg cgaaaccggc ctgattgtta cgcccttact gcccggcgat
     1321 tcgctgctgt ttgccgccgg cgggattgcc gcgctgggcg gtatggatat tcatttgatg
     1381 gttgcattat taagccttgc cgccatactc ggcgacgcgc tcaattttac cgtcggcaaa
     1441 tatttcggcg gcaggctgtt cgccaacccc gattctaaaa tcttccgacg tgaatatctc
     1501 gataaaaccc accgttttta cgaaaaacac ggcggcaaaa ccatcatcat tgcccgtttt
     1561 atgcccatcg tccgcacctt cgcccccttc gtcgccggta tggggaaaat gcactatgcc
     1621 aaatttatcc gctacaacat catcggtggc ttgttgtggg ttatcctgtt ttcttacgcg
     1681 ggctatttct ttgccaactt ccccgtcgta aaaaacaatc tgggcttggt gatgggcggc
     1741 atcattattg tttccgtcct gccgggtgcg gttgaaattg cccgcgccaa acttgcggta
     1801 aaatccaaac ggtaactgtt gagaatttgt atcatgaata tattatctgt agaaaacgct
     1861 tcttttgcca tcggccacgt tgccttgctc gacaaaactt cttttcaact cgacagcggc
     1921 gaaaaagtcg gcttaatcgg acgcaacggc gcaggcaagt cttcgttttt aaaaatcctc
     1981 gccggtttgc aaaagctcga cgacgggcag attattgttc aaaacaacct caaaatcgtt
     2041 tatgtaccgc aggaatcctt ttttgataag gacgcaaccg tatttgatac cgttgccgaa
     2101 ggtttgggcg aaattcgcga tttattgcgc cgttatcatc atgtcagcca tgatttggag
     2161 cacaattcag acgacgtttt attaaaagaa ctcaacgaat tacaactcga aatcgaagcg
     2221 aaggacggct ggaagctgga tgcggcagtc aagcagactt tgggcgaact cggtttgccg
     2281 gaaaacgaaa aaatcggcaa cctttccggc ggtcagaaaa agcgtgtcgc cttggcgcag
     2341 gcttgggtgc agaagcccga cgtattgctg ctggacgaac cgaccaacca tttggatatt
     2401 gacgcgatta tctggttgga aaacctgctc aaggcgtttg aaggcagctt ggtcgtgatt
     2461 acccacgacc gccgtttttt ggataatatc gccacgcgga ttgtcgaact tgaccgcggc
     2521 attctacgtt cctatcccgg ctcgttctct aaatacagtg agaaaaaagc gcaagagttg
     2581 gcagtcgagg cggaacataa ccgcttgttt gacaaatttc atgctcagga agaagcgtgg
     2641 atacgcaaag gcattgaagc gcgccgtacc cgtaacgaag gccgcgtgcg ccgcttggaa
     2701 gagctgcgcc gccaacgtgc agaacgccgc aacgtgcagg gacaggtcaa ctttaagctc
     2761 gacagcggtg agaagagcgg caaaatcatc gccgaattgg agcacgcttc gtttgcctac
     2821 ggcggcaaag tcattatgga caaattctcc gccatcttgc agcgcggcga caaaatcggc
     2881 ttaatcggtc caaacggcat cggcaaaacc acatttttaa aactgatttt gggcgaattg
     2941 cagccgacct acggcagaat ccgtatcggc agcaagcagg aagtcgccta tttcgaccag
     3001 ttccgcagcg cgttgaatga aaacgacacc gtgttttaca cgctcggaca gggcaatgat
     3061 tacgttgaag tcggcggtaa gaaaaagcat gtgatgagct atttggaaga tttcctgttc
     3121 ccccctgccc gcgcgcaaag tcccgtttca tcgctctccg gcggcgaacg caaccgcctt
     3181 ttgctggcaa aactctttac ccgtcctgct aatattttga ttttggacga accgaccaac
     3241 gacttggata tcgataccca agagctgctt gaagatttgc tgcgcgatta tcaaggcacg
     3301 gtattccttg tctcacacga ccgtatgttc ctcgataacg tgattaccca aagcattatt
     3361 tttgaaggaa atggaaggtt aaaagaatat atcggcggct atcaggacta catcgacgca
     3421 aaatcacgcg aagcgaagat tcagacggaa tccgcaccca aaacagccga tgccgaaccg
     3481 gtgaaagaaa aacccaaagc caaccgcacg gtcaaactgt cctacaaaga acagcgcgaa
     3541 ctcgacgccc tgcccgacga aatcgccgcc ttggaagccg aacaggcaga aatcaacgcg
     3601 cagctttccg atcccgaaat cttcaaagat tacgaaaaag ccggcgcact gcaaagccgc
     3661 gccgaagaaa tcgaaatgct gcttttggaa aagctggaac gctgggagct gctggaaacc
     3721 aaacagaacg gcaatgccgt ctgaaatcaa acaggaagaa aaaaatgttc aaacgacctg
     3781 aagaaatcat tgtcctgata cttgccgtcc tctggattgc cggtacgtat tttctcgccg
     3841 ccctgtttgg agcggatgct tataccgtgt taaaaatcac tgcccttacc ctgctttggt
     3901 ctgccgcatc attcctcctt tggcaaaaaa acctcagccg gcatatctgg ccgctgctgc
     3961 tcggctgcct gactgcctgt tggtggccgt atctgaatca atcggtcgca cccggttctt
     4021 taccttggta tgcatcatgg acgttcaaaa ccatcttagc cctgattccc gtaaccgcag
     4081 gttatctgtt taaatggaaa caataccggc agcgcaaaac aaagccgtaa gcccgtcctt
     4141 ttcagacggc atctccggtc agactgtcaa caattccgat aaaatgctat actcgcgccc
     4201 gttgccgcgc cgtccgaaac cgccctttgg gaaagcagaa atgatcagca aacaagaata
     4261 ccaagcccaa gccgctcaag gctacaaccg catcccgctc gtgcaagaac tccttgccga
     4321 tttggatacg ccgctttccc tctatctcaa actcgccaac cgcccctata cctacctgct
     4381 cgaatccgtt gtcggtggcg aacgtttcgg ccgctattct tttatcggcc tgccttgcag
     4441 ccactatctc aaagccagcg gcaaacacgt cgatgtttat caaaacggcg aaattgtcga
     4501 gcaacacgac ggcaaccccc tgccctttat cgaagccttc cacaaccgct tcaaaacacc
     4561 cgaaatccca agcctgccgc gctttaccgg cggactggtc ggctacttcg gttacgaaac
     4621 catctacaat tttgaacact tcgcccaccg cctgaaaaac accaccaaag ccgacccgct
     4681 cggcacgccc gacatcctcc tgatgctctc gcaagagctt gcagtagtgg ataacctaag
     4741 cggtaaaatc tacctgattg tttacgccga tccgtcgcag cccgacggct acgaacgcgc
     4801 acgcgaacgc ctcgaagaca tccgtacaca gttacgccaa agttgcgcca tcccgctttc
     4861 gctcggcagc aaacacaccg aagccgtcag cgaattcggc gaagagccgt tcaaagcctg
     4921 cgttaacaaa atcaaagact acatctttgc aggcgactgc atgcaggttg tccccagcca
     4981 gcgcatgagc atggaattca ccgacagctc gctcgccctc taccgcgcct tgcgcacgct
     5041 caacccttcg ccttatctct tctactacga tttcggcgat ttccacatcg tcggctcctc
     5101 gcccgaaatc ctcgtccgcc gcgaacgcga cgatgtcatc gtccgcccca tcgccggcac
     5161 gcgcctgcgc ggcaaaaccc ccgccgaaga ccttgccaac gaacaagatt tgttaagcga
     5221 tgccaaagaa atcgccgaac acgtcatgtt gattgactta ggacgcaacg acgtcggccg
     5281 catcagtaaa accggcgaag tcaaagttac cgacaaaatg gtgattgaaa aatactccca
     5341 tgtaatgcac atcgtttcca acgtcgaagg ccgtctgaaa gacggcatga ccaacatgga
     5401 catcctcgcc gctaccttcc ctgccggcac actctccggc gcacccaaag tccgcgccat
     5461 ggaaatcatc gaagaagtcg aaccaagcaa acgcggcatc tacggcggtg ccgtcggcgt
     5521 atggggtttc aacaacgaca tggatttggc aatcgccatc cgcaccgccg tagtaaaaaa
     5581 caacacgcta tacgtccaaa gcggcgcggg cgtggttgcc gactccgatc cggcctccga
     5641 atggcaggaa acacaaaaca aagcccgagc cgtcatacac gccgcgcaga tggtgcagga
     5701 agggttggat aaatgactta tgaaaaactt tatgagcttc gccaaacact cagaccgact
     5761 accaaagacg gtctctatac agataatgaa aaaagtcgag aaatcctaac ggttagatgg
     5821 agcggcaaca cagaaaatcc taaaaatttc tcagctgttg ctataggaat taatccaagt
     5881 aaagcaaatg atgaaagaag cgacaaaacc cttacccaac ttgcacgttt cttggatatg
     5941 tacggtttta ccaacttcaa aatgttaaat attttttcca gttactctac acagcaaaca
     6001 ggaattaggg caaacaccca aaccgatttt tccaaattta aaggatgttt ggaagatgct
     6061 gatatgatta ttcttgcatg gggtacagac cgcggtgctt ataaggatga aaaaaacaga
     6121 atactggaat ttctaaaagc agaaaaattt atggaaaaag tattctgtat ttctgaaact
     6181 ggtaattcca gcgatacaag acatccgtca agaattagtt atagctatca acttgtacaa
     6241 tttgaagagt cagcatgaaa aacatatctc tttctccgcc cgccatgctt ggcatcctcg
     6301 gcggcggaca attaggcaga atgtttaccg ttgccgccaa aaccatgggc tacaaagtaa
     6361 ccgttctcga ccccgatccg gacgcgccgg cagcagaatt tgccgactgt catttatgcg
     6421 cgccgtttaa cgaccaagct gctttggacg aattggcaaa atgcgcggcg gtgaccactg
     6481 aatttgaaaa cgtcaatgcc gatgcgatgc gttttctcgc caaacacacc aatgtttccc
     6541 ccggcagcga ctgcgttgcc atcgcgcaaa accgcattca ggaaaaggca tggatacgca
     6601 aagcaggatt gcaaaccgcg ccgtatcaag caatttgcaa agccgaagac atcactgaag
     6661 aaagcataca atttctgccc ggcatcctga aaaccgctac attgggctat gacggcaaag
     6721 gccaaatccg cgtcaaaacg gtggatgaac tcaaagccgc gtttgccgaa caccgcggcg
     6781 tggattgcgt tttggaaaaa atggtggact tgcgcggcga aatttccgtt atcgtatgcc
     6841 gtctgaacaa tgacaacgtg caaactttcg atcctgccga aaacattcac gaaaacggta
     6901 tcctcgccta ctccatcgtc ccagcccgac tgagtgccga cattcagcaa caggcgcgac
     6961 aaatggcgca gcgtttggcc gatgaattga actacgtcgg cgtattggcg gtagaaatgt
     7021 ttgttgtcgg cgacacgcat gaattggtcg tcaacgaaat cgcgccgcgt ccgcacaatt
     7081 ccggccacca taccgtcgac gcctgcgcgg cagaccaatt ccagcaacag gtccgcctga
     7141 tgtgcaacct gccacctgct gacaccaaat tgctgagttc ttgctgtatg gcgaatattt
     7201 tgggcgacgt ttggcaggaa gacggcggcg aaccggattg gtttcccctg caaagccggc
     7261 cggacgcgca cctgcacctt tacggcaaaa aaaccgcgca caaagggcgg aaaatgggac
     7321 actttaccgt tttaaccacc gattcggaca ccgcatttca agaagcaaaa aaactgcatc
     7381 agtccctata atccgaccaa cggcacagat gccgtctgaa gccgccgaca taccgccatg
     7441 agcctgctga cactgttacg ccccgccacc gtgcaagact gcaaagacat cttcaaagtc
     7501 cacctgcact ccgtccaata cacctgcatc ctcagctaca acgaacacgc cctaaaagta
     7561 tgggaaggct tgctaaacac agaaagctac ctgccgacca tttccgatcc cgacaaagca
     7621 ctttgggttg ccgaatacaa aggcaacata cagggatttt tccaaatcga ctgccaagaa
     7681 gcacaactcg atgcgcttta cgtccacccc ctgttccaca acctcggact gggcactgcc
     7741 ctacttcatc aggcggaaac catcgcccac aaatccggtt tgagctttct gaaactctac
     7801 gcatcgctca actccgtccc gttttacctt ctcaaccgct acgaatcgct cggttcggca
     7861 gtattgcagc ttgaccccag catcaaaatc aaatgcgaac tgatgcggaa acacctctga
     7921 tccgtcctta taaccaaaac tgataatctt caaatcaaac attatttcca caagcgcaga
     7981 cagccgtata aagttgcccc atctaccaac cgacaggaca agcatatgaa aacctacgca
     8041 ccggcactct ataccgcagc cctgctcacc gcctgcagcc ccgcagccga ttcaaatcat
     8101 ccgtccggac aaaatgcccc ggccaatacc gaatccgacg gaaaaaacat taccctgctc
     8161 aacgcctcat acgatgtggc acgggatttt tacaaagaat acaacccctt atttatcaaa
     8221 acataccaat ccgaacaccc cggcacatcc gtcagcatcc aacagtccca cggcggctcc
     8281 agcaaacagg cattatccgt agccaacggc cttcaagccg atgtcgtaac catgaaccaa
     8341 tcctccgaca tcgacctgct cgaaaaaaaa ggactggtag aaaaaggctg gcaacaagcc
     8401 ctccccgacc acgccgcgcc ctacaccagc actatggttt tccttgtccg aaaaaacaac
     8461 cccaaacaga tccgcgattg gaacgacctt gccaaagacg gcgttaacat cgtcatcgcc
     8521 aatcccaaaa cctcgggcaa cggacgctac gccttcctcg gcgcatacgg ttacggtctg
     8581 aaagccacca acggcaacga acaggaagcc caaaaactcg tcgcatccat cctcaaaaac
     8641 acccccgttt ttgaaaacgg cggacgcgcc gccaccacca ccttcacaca acgcaacatc
     8701 ggcgacgtac tcattacttt tgaaaacgaa gccaactacg tcagcaaaaa actgacccaa
     8761 ggacagtttg aaatcgtcta tccgagctac accatttccg ccgaaagccc cgtcgccgtc
     8821 gtcaacagcg tcgtcgcgaa aaaaggcacg caaaaaaccg cccgcgccta tctcgaatac
     8881 ctttggagcg aaccggcgca agaactcgcc gcctcactct acctgcgccc ccgcaacccc
     8941 gaagtattgg caagacacaa agccgacttc ccagacttag actcattctc tcccgaagaa
     9001 aaattcggcg gatgggacaa catcatgaaa acctacttcg ccgacggcgg catattcgac
     9061 cgcctgaccg cacaaaaatg accgctatgc cgtctgaacc gcctttcaga cggcattcca
     9121 cacatcaaaa cagaaaaacc gcgcccaaaa tcatcatcac aagacacaac ggcaaaccgg
     9181 atacaaaacc caacaccgcc gccaaaatgc cgtctgaagc cgtcggcgca accatcctta
     9241 tctggggcgc gcttcagggg cgcataactt tccttaacca tcccttttga cagccaaata
     9301 aacagtgctt ttgcctgtca gaaacaaaac caatattttg cagttcgtat cggaacattt
     9361 atagtggatt aacaaaaacc agtacagcgt tctcgc
//
LOCUS       DAXHKX010000066         9350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHKX010000066 DAXHKX010000000
VERSION     DAXHKX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.066"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            109..318
                     /locus_tag="ACFMWZ_001189"
     CDS             109..318
                     /locus_tag="ACFMWZ_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC5935154.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            407..1621
                     /locus_tag="ACFMWZ_001190"
     CDS             407..1621
                     /locus_tag="ACFMWZ_001190"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC5935155.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1708..4287)
                     /gene="clpB"
                     /locus_tag="ACFMWZ_001191"
     CDS             complement(1708..4287)
                     /gene="clpB"
                     /locus_tag="ACFMWZ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC5935156.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLSSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4547..5557
                     /gene="trpS"
                     /locus_tag="ACFMWZ_001192"
     CDS             4547..5557
                     /gene="trpS"
                     /locus_tag="ACFMWZ_001192"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC5935157.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANSENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5563..5904
                     /locus_tag="ACFMWZ_001193"
     CDS             5563..5904
                     /locus_tag="ACFMWZ_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935158.1"
                     /translation="MPSEPLKGLPEQNVVRLIGKHPNDLEVISGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6224..6547
                     /locus_tag="ACFMWZ_001194"
     CDS             6224..6547
                     /locus_tag="ACFMWZ_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935159.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6756..8264)
                     /gene="ppx"
                     /locus_tag="ACFMWZ_001195"
     CDS             complement(6756..8264)
                     /gene="ppx"
                     /locus_tag="ACFMWZ_001195"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC5935160.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPRAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFIIGSTLRPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDSEWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8381..9112)
                     /locus_tag="ACFMWZ_001196"
     CDS             complement(8381..9112)
                     /locus_tag="ACFMWZ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC5935161.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLHLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSQHLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTLVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT         2158 a         2420 c         2472 g         2300 t
ORIGIN      
        1 ttttgttaat ccgctatatc gatttgaatt tttcagaaaa tgaagcggac gtttgggcgg
       61 aggcaacttg tttgataaga tagcaatatt tcaaaacgga gaaagatcat gccttatgtc
      121 aatattaaag taaccggcgg caaggaagca ccgactgccg cgcaaaaagc ggaactgatc
      181 ggcggcgtaa ccgaattgct ggcacgcgtg ctgggcaaaa atcccgaaac aacggttgtc
      241 gtgattgacg aagtggatac cgataactgg ggaataggcg gcaaaagcgt cagcgaacgg
      301 cgcaaagagg gcaggtaaaa agcctgaaaa tctcggtttg atgctttaaa ttccgcgtga
      361 aaaagagtac attcccaccc attgcccaaa atttacggaa cacatcatgg ataaatttcc
      421 caagtctgca aagctcgatc acgtctgtta cgacatacgc gggccggttc acaaaaaagc
      481 ccttcagttg gaagaggagg gcaataaaat ccttaaactc aatatcggca accctgcgcc
      541 gttcggcttt gaagcccctg atgaaatctt ggtcgatgtc atccgcaacc tgccgacttc
      601 gcaaggctat tgcgattcta aagggctgta ttccgcccgc aaagccattg ttcactacta
      661 tcagaccaaa ggtttgcgcg atattacggt tgatgatgtc tatatcggca acggcgtgtc
      721 cgagctgatt acgatgtcta tgcaggcgtt gctcaacgac ggcgacgaaa tcctgattcc
      781 cgcgcccgac taccctttgt ggacggcggc ggcaacgctt gcgggcggta cggtacgcca
      841 ttatctgtgc gacgaagaaa acggctggtt ccccaacctt gccgatatgg aagccaaaat
      901 cacgcccaaa accaaagcca tcgtcgtcat caatcccaat aatccgacag gcgcggtgta
      961 cagcagggaa atcctgttgg aaatcgccga actggcgcgc aagcacggtt tgattatttt
     1021 cgccgacgag atttacgata aaatccttta tgacggcgcg gttcaccacc acatcgccgc
     1081 gcttgccccc gaccttttga cggtaacgtt caacggtttg tccaaatcct accgcgtcgc
     1141 cggcttccgc caaggctgga tggtgctcaa cgggccgaaa catcacgcaa aaggttacat
     1201 cgagggtttg gatatgctct cgtccatgcg cttgtgtgcc aatacgccga tgcagcacgc
     1261 gattcagacg gcattgggcg gctatcagag cattaacgaa tttatcctgc cgggcgggcg
     1321 gcttttggaa cagcgcaaca gggcgtggga actggtcagc caaatccccg gcgtgtcctg
     1381 cgtcaaaccg atgggcgcga tgtatatgtt cccgaaaatc gataccgaaa tgtaccgcat
     1441 ccgcgatgac atgaaattcg tttacgattt gctggtgcgc gaaaaagtct tgctggtgca
     1501 gggaacgggg tttaattgga tcaagcccga ccatttccgc attgttacgc tgccttacgt
     1561 ccatcagatt gaagaggcga tgggcaggct ggcaagattc ctgcaaacct accgccaata
     1621 aggggacggg ctgtctgccg aggataaaaa atgccgtctg aaacggagat tcccgtttca
     1681 gacggcattt ttaacagcag gaacgaatca ggcaaatttc agtctgtcgc cgtcggcttc
     1741 caccctgatt tcgctttcgg gcgcatagtt tccggcaagc agggcttttg ccagcgggtt
     1801 ttcgatttcc gactggatgg cgcgtttgag cggacgtgcg ccgtaaatcg ggtcgaaacc
     1861 ggctttggcg atgatgtcca gagcggcatc ggaaacagtc aggcggaggt tttgtttttc
     1921 caaacgtttt tccaaacctt taagctggat tttggcaata ctgcggatgt ttgcctgatc
     1981 cagtccgtgg aacacgacca cttcgtcgat gcggttgatc atttcaggac ggaaatgttc
     2041 tttcacatcc tccatcacaa cttctttcac cgcttcgtag tcttgagtac ccatttgttg
     2101 gatgtgctgg ctgccgatgt tggaagtcat cacaatcacg gtatttttaa aatctaccgt
     2161 cctgccttgt ccgtcggtca agcggccgtc atccaatact tgcagcagga tgttgaacac
     2221 atcgggatgg gctttttcca cttcgtccaa aagaattacg ctgtacggtt tgcggcggac
     2281 ttgttcggtc aggtagccgc cttcttcgta gccgacatag cctggaggcg cgccgatgag
     2341 gcgggcaacg gcgtgttttt ccatatattc ggacatatcg atacgaatca gatgatcttc
     2401 gctgtcgaac aggaagcctg ccaatgcctt gcacaattcg gtcttaccca cgccggtcgg
     2461 gcccaagaac aggaagctgc cgtagggttt attcgggtcg gcaagaccgg aacggctgcg
     2521 gcggatggcg tcggacacgg cacgcacggc ttcgtcttga ccgactacgc ggcggtgcaa
     2581 cacttcttcc attttcagca acttgtcacg ttcgccttcc atcatttttg acacaggaat
     2641 gccggtcata cgggaaacaa cttctgcgat ttcctctgcg ccgacattat tacgcaagag
     2701 tttgtttgcc ggttttgtgc tgtccgtatc tgcccgttcg gcggctgcac gctgtttttc
     2761 caaatgctcc aaatcttcat acatcaattt tgaagccagt gccaaatcgc cttgccgttt
     2821 tgcctgttcg attttaattt tgacttcgtc aatttgtttc ttaatattag cagcaccgtc
     2881 tgaaattgct ttttcggctt tccagatttc gtctaaatcg gcgtattctt tttgcagacc
     2941 gttgatttcc tcgtctatca gttccaaacg ttttttgctg gcatcgtctt tttctttttc
     3001 aacgtgcgcc ttttccatcc gaagctgaat tagacggcgg tcgattttgt ccattgcttc
     3061 cggcttggtt tctttttcca tcttgacacg gctggcggct tcgtcaatca aatcaatcgc
     3121 tttatcgggc aggaagcggt cggtgatgta gcggtcgctc aactccgctg cggcaacgat
     3181 agcagggtcg gtaatatcga taccatggtg gatttcataa cgctcctgta aaccgcgcaa
     3241 aatagcgatg gtgtcttcca cgcttggctc gccaaccaat actttttgga aacggcgttc
     3301 aagtgccgcg tctttttcga tgtattggcg gtattcgtcc aaggtagtcg caccgataca
     3361 gtgcagttcg ccgcgtgcca aagccggttt caacatattg cccgcgtcca tcgcgccgtc
     3421 ggttttacct gcgccaacca aagtatggat ttcgtcgatg aaaatcagag tgttgccatc
     3481 gtctttcgcc aagtcgttca acacgccttt caagcgttct tcaaattcgc cgcgatattt
     3541 cgcgccggca atcaaagccg ccaagtccag aaccagcagg cgtttgttgc gcagggattc
     3601 gggtacttcg ccgttgacga tgcgttgtgc caaaccttcg acgatggcgg ttttacccac
     3661 gcccggttcg ccaatcagca cagggttgtt tttggtacgg cgttgcaata cctgaatcgc
     3721 acggcggatt tcgtcgtcac ggccgataac ggggtcgagc ttaccgtctc gggcgcgttg
     3781 ggtcagatcg agcgtgtatt ttttcaaagc atcgcgttgg tcttcggcat tggcatcgtt
     3841 cacgttttgt cctcctcgta ccgcgtcaat cgcggcattg atgttttgtt cggtcgcgcc
     3901 ggcttctttc aaaattttgc cggccgcatc gttctgctgc accaaggcaa gcaggaaaag
     3961 ttcactggcg atataggcat cgccgcgttt ggttgcggct ttgtccatca ggttcaacac
     4021 cgcctgcaat tcgcggctgg gcagaatgtc gccgccctgg ccggacactt tcggcaggct
     4081 gctcaaatgc tgctgcaaac gctgtttcac ctgcggcacg ttcacgcccg aatgcgccag
     4141 caacgcggcg gctccgctgt tttggtcgtc aagcagggct tttaacacaa agcctgcttc
     4201 cagatagctg ccgtccgcag ccaacgccaa actctgagct tctgcaaggg cttgttggaa
     4261 tttggcggtt aatttgtcgt atcgcatttt tgtttccttt tcaaaatgtc cgctgtcgaa
     4321 gcctatatgt gcataattgt ggataactca agttctgttt tctgtttttc tatatttaat
     4381 tcgatatatc attgaattta aagtatataa aaatgtataa taatgtgtat aactatatct
     4441 tcttaatatg gaaaagtctg ttgtcggctg gatgtaggtg gcaaatcggg tataatcggc
     4501 acatcttttt ccctttcaga cggcattgat gccgcaagga cattttatga gcaaaaaacg
     4561 agttctgacc ggcgtaacca ccaccggcat cccgcatctg ggcaactacg tcggtgctat
     4621 ccgccccgcc gtccgcgcgg cgcaaaaccc tgataccgaa tccttcctct tcctcgccga
     4681 ttaccacggt atcatcaaat gccacgagcc ggagatgatt caccaatcca cccaagccgt
     4741 tgccgccact tggcttgcct gcggactcga ccccgagcgc accaccttct accgccaaag
     4801 cgacattccc gaagtgatgg aattgaactg gattctgacc tgtatcaccg ccaagggttt
     4861 gatgaaccgc gcccacgcct acaaagccgc cgtgcaggca aattcagaaa acaatcagga
     4921 agatcccgat ttcggcgtgg aaatgggttt gttcagctat ccgattctga tgaccgccga
     4981 catccttatg ttcaacgcca acgaagtgcc cgtcgggcgc gaccaaatcc aacacgtcga
     5041 aatggcgcgc gacatcgccg gccgcttcaa tcaccgcttt aaagaaatct ttaccctgcc
     5101 cgaagtgaaa atcgacgaaa acgtcgaact cttggtcggt ttggacggac gcaaaatgtc
     5161 caaatcctac ggcaacacca ttccgctttg ggaaaacgac aaaaaaaccc aaaaatcggt
     5221 caacaaaatc atcaccaata tgaaagagcc gggcgagccg aaacagcccg acgaaagccc
     5281 attgtttgaa atctacaaag ccttctccac gccgtctgaa accgcagcgt tcacgcaaat
     5341 gcttgccgaa ggcttggctt ggggcgaggc aaaaaaactt ttggcggcga aaatcaacgc
     5401 cgaactcgcc gaaccgcgcg aacgctacaa cgagctgacc gccgaccctt cgcaaatcga
     5461 agagattttg caggcaggcg cgcaaaaagc gcgtaaagaa gcacgcgaat tgttggacaa
     5521 agtacgcgat gcggtcggca tccgcccgtt gaaataaacc cgatgccgtc cgaacctctt
     5581 aaaggcttgc cggaacaaaa cgtcgtccgg ctgatcggca agcatcccaa cgacttggaa
     5641 gtcatcagcg gcaaatgtat ggaaaccgac ggaaagggcg cgccttcggg ctgggcggca
     5701 aacggcgtgt gccatacctt gtttgccaaa ctggtgggca atatcgccga agacggcggc
     5761 aaactgacgg attacctgat ttcgcattcc gccctgcaac cctatcaggc aggcaaaagc
     5821 ggctatgccg ccgtgcagaa cggacgctat gtgctggaaa tcgacagcga aggggcgttt
     5881 tatttccgcc gccgccatta ttgaggtatt cggacatccc ggaatatatt tgggtttttc
     5941 aaaccttgcg aaaaaagtcc gcaccgtcgg gaaactcaaa aggaggggga tatgtgttac
     6001 aattttccga actgttttca taaaatagtt ttcggacgtg tttcaatatg gcattgatgc
     6061 cgccttattg ttcgagaaaa aaaaccttta tatttaaata taatgggttt taactaaacg
     6121 ggaaaccgtt ttctctccgg ccgatgggca aaatcagccg attgatggaa cacggtccga
     6181 tttttaagca aaaccttaat caacaactca agaaggataa gacatgaaaa aattattgat
     6241 tgccgcaatg atggcggctg ccttggcagc ttgttcgcaa gaagccaaac aggaggttaa
     6301 ggaagcggtt caagccgttg agtccgatgt taaagacact gcggcttctg ccgccgagtc
     6361 tgccgcttct gccgtcgaag aagcgaaaga ccaagtcaaa gatgctgcgg ctgatgcaaa
     6421 ggcaagtgcc gaggaagctg taactgaagc caaagaagct gtaactgaag cagctaaaga
     6481 tactttgaac aaagctgccg acgcgactca ggaagcggca gacaaaatga aagatgccgc
     6541 caaataattt gttgccttgg caaaaatgat gggatgccgc ctgccggcaa cccaaacgaa
     6601 accgcctgaa gattttcagg cggtttttga gttgtatgcc ccttgttttt tacgcctgca
     6661 tgaccgttcc gagcagatgt gccgcgaata tcgggatgtt gcggacaggc aaatgccgtc
     6721 tggacagggt ttggacggca tttgttccgt aaggttcaga cgacctcgac tttaaacggc
     6781 atattgattt tttgccattg gacgctttca tattccaatg cgtcggcaat caggggatgg
     6841 cgttccagcc attcactgtc aatacgcagg atgaagccgc agctttccgt atccgtgcgc
     6901 aactgcatgt ttttcgggaa agacaggtct tggcgcgaac ggcagaacag ggcagcaagg
     6961 cgcagggaca aaacggcata ccacaacatt tcgttggtgc cgatgatgcc gctcattttt
     7021 ttcatatcgc cgcgatgacc gatgaccagt tgggcaagta tggtctgttc tttgcgtgaa
     7081 aagcccggca tatcggcgtt ttcgaggatg taggcggaat gcttgtgata gccggtgtgg
     7141 gcgatgtcca aaccgatttc gtgcagcgcg gcggcgcgtc cgagatactg ttgccacaag
     7201 gcaagctctt gaactgtaac gtttttagcg tggcagaggc tgtccataaa ggtttgcgcg
     7261 gtctcggcgg tgcgtttcgc ctgattgagg ctgacgtggt agcggtgttg gaactcggca
     7321 accgtttgtc cgcgcatatc ttcgtttaaa ccgcgcccga tcaaatcgta aaacacgccg
     7381 tcgcgcaggg cggcttcggt tacggtcatc ctgtcgagtt tcatttcctc aaacgccgcc
     7441 atcatcacgg caagtccgcc ggcaaaaact tcgatgcgtt ccggtttcag gttttcaaat
     7501 ttggcttttt tgaccgaacc ggcttcgatg atgcgttcgg cgagggcgcg cattcctttg
     7561 taggtaatgt ccgcctcttg gggcatttcg gcggcaagca cgtcgcggat ggattttgcc
     7621 gaacccgatg tgccgacggc gaaatcccaa ccttcgcgcc tcatattttt gctgatgcgc
     7681 tggatttcgt tgcgggcggc ggaaatggca gattggaagt ctttggcggt gattttgttt
     7741 tggaagaagc gcaggctgta ggttacgcag cccaagggca ggctttcggt aatgtcgggg
     7801 cgcagggttg aaccgataat gaattctgtc gagccgcccc cgatgtcgat aaccagcatt
     7861 ttgccgccgc cgggggggag ggtgtggatc acgccggtat aaatcagccg cgcctcttcg
     7921 cgcccggcga tgatttcgat ggggaaaccc agtgccgctt cggcacgggg aaggaaatct
     7981 gcgatgtttt tggcaacgcg gaatgtgttg gttgccacgg cgcgcacctg ttcggggcgg
     8041 aagccgcgca ggcgttcgcc gaattttgcc agacagtcca aagcctgttc ttgggaggcg
     8101 gcactcaggt ttttctgttc gtccagtccg gcggcgaagc gcaccatctg tttgaacgaa
     8161 tcgatgactt ttaattgtcc gttgttgttt tcgcaaatct ggaggcggaa actgttggaa
     8221 cccaaatcga cggaggcgag gatgtttgcg ggggtggtgg tcatggcgga tatcggtggg
     8281 ggaaaaacgc aatgttactc tgacggcgca ggcgttgaca ataaatgatg cggctgtttt
     8341 tgattctgct cgcggatgtt gccgacggca ttttttgcgc ttatttgaaa tccttttcca
     8401 cgctcatgaa aatctgcatg tttttgcgtg tgtaaaaact tttcatattg ctgtcgattt
     8461 tcagatagcg gaaattgagt tgcggcgtaa agcccttcca agagattttg tcgtgccaca
     8521 acgacaggtt tgcctgatat tcgtggtctt tgcgcgggaa gcggtacaca agggtcccgg
     8581 gtgcatcaaa agtcctgcgg gtgtagcgca ggtttgcccg cagacccaag ccgccgtcga
     8641 acgttttgac cgctccgaca cgcaaaccct tgcggatgga agcctgttcc gcctccttcg
     8701 tcaagtcgtg cgaccagtcc gcaccgccgt aaagcagcca gtctttcggc gcggaataca
     8761 tcagcgtcgc gcccgccagc ggcatatggc tgtcgtatcg ggcggcggtg cggtcttcct
     8821 gataatgctt ccacatattg cccgcgttta gtgtcaaccg ccagcgttcg ctcaagtgtt
     8881 gggagaaatc ggcattaaag ccgccgacga aattgtatcg gctgccgcct aagaggtttt
     8941 gcccgacaaa cggcacgatg ccgaacgagc gcgttaccga acgattttta tagccgagcg
     9001 acaggtgcag gcactgttcg ctgaaatctt tgttatccca ataatgcacg ccgccgccgc
     9061 tgatgccgcc gtagaggaaa tgatgcccgc ccgcattgat ttcgcgcgac acgcctaagc
     9121 cctagcgcaa accgtgtgcc ttttgcggca ggctgtcggc ggttttcgtc cagcttctgc
     9181 ccgcaaattc aatcgttttt ttggacgaag cgttgtttat agcggattaa caaaaatcag
     9241 gacaaggcga cgaagcctca gacagtacag atagtacgga accgattcac tcggtgcttc
     9301 agcaccttag agaatcgttc tcttcgagct aaggcgagac aacgccgtac
//
LOCUS       DAXHKX010000067         9299 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHKX010000067 DAXHKX010000000
VERSION     DAXHKX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.067"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(91..1371)
                     /locus_tag="ACFMWZ_001197"
     CDS             complement(91..1371)
                     /locus_tag="ACFMWZ_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC5935162.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /gene="tmk"
                     /locus_tag="ACFMWZ_001198"
     CDS             complement(1593..2213)
                     /gene="tmk"
                     /locus_tag="ACFMWZ_001198"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC5935163.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="ACFMWZ_001199"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="ACFMWZ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC5935164.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="ACFMWZ_001200"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="ACFMWZ_001200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC5935165.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4118..5404
                     /locus_tag="ACFMWZ_001201"
     CDS             4118..5404
                     /locus_tag="ACFMWZ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC5935166.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5552..8077
                     /gene="ligA"
                     /locus_tag="ACFMWZ_001202"
     CDS             5552..8077
                     /gene="ligA"
                     /locus_tag="ACFMWZ_001202"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC5935167.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     IQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYGHMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINEAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8134..>9299
                     /gene="hemW"
                     /locus_tag="ACFMWZ_001203"
     CDS             8134..>9299
                     /gene="hemW"
                     /locus_tag="ACFMWZ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC5935168.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         2184 a         2798 c         2347 g         1970 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacgccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac aatacattgt tcacttggtt
      421 cgggaagtcg gagcggccgg taccgataac cacgtccgga cgggtttctt tcgccagcgg
      481 cggcaggatt tccggattcg ggttggccat ggcgaacacg atgggttttt cgttcatcgt
      541 gttcaacatt tcgggggtca gcaggttcgc gccggagagg cccaagaaga tgtctttacc
      601 tttgacggca tcggcaagta cgcgctggcc attgtcttca acggcgtaga attttttgga
      661 ttcgtccatg cggtctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcggcgg cggtaatgat ggccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttaccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacggc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccaaaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 aacgggtaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggtggatgc gcttgtttca atatttgccg cattaacagc cgaaatgtcc gtccaacact
     1621 ttttctatgt tgtttctgac ttcatccaag ctgcggttac tgtcgataat ggcgtaccgt
     1681 tcgggacagg cggcggcacg gttcagataa acgctgcgca cacgcatgaa gaaatctgcc
     1741 tgttcctgct cgaaccggtc tttctcgcgc gactgtccga tacgcgccat cgacacttcc
     1801 agcggcacat ccaacagcag ggtcaaatcg gggcgcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggttgcg
     1921 tcggtaaaac ggtcggacac gacatggatg ccgtctgaaa gtgcaggcaa tatcacgtct
     1981 tcaatatgct gcattctggc ggcgaacatc atcagtgttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggttcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tccgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcgaacg gcggcgttgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtacc gtccattttg gacacgaaat acaggtattt ttcaccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg cgatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcaggc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccctgtt tccttttcga
     2641 tcaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcattcagt cggcgttgca tcgccttgta ggcgatttgg taaatccgta
     2761 aatcgctgcc gcccgcatcg atttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcaggggca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgttcgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc agtctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt aatcctgtat gccctgccgt
     3181 tgtctttagg gacgaaaagc agcgcggcga aaacggctgc cgatacggtc aaaaaaacgg
     3241 cagaccattt caacaatttt ctcaacatgg taggattccc agcattcgga atacgtctta
     3301 aaattcgggc gacagtataa cccaaattgc ccgccggaca ggaagaacat catggacaat
     3361 cacgccgaag cacactggca aaacggctgg cttcaaagca tacgccatac cccgtcgccc
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatatgg tacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttttta
     3601 atcaaacgcg acggcgaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatcct cgtttcgcgg acgggaaaaa tgcaacgcat tttccatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc ctttaccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccgcta ccccatcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg tcattttttc gactggcggc ggatacgggg gaaagggttt
     3901 cccgtggaca gaaatgccgt ctgaaaacca cagattttag acggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 accggccgtt ccatcagact gccggaaacg caagcccgat ttaaccgcag tacccgcact
     4081 tcgcgcatcc tgcatcccga aatttaaagt gattgccatg atttacatcg tactgttcct
     4141 cgccgccgtc ctcgccgttg tcgcctacaa tatgtatcag gaaaaccaat accgcaaaaa
     4201 agtgcgcgac cagttcgggc actccgacaa agatgccctg ctcaacagca aaaccagcca
     4261 tgtccgcgac ggcaaaccgt ccggcgggtc agtcatgatg ccgaaacccc aaccggcggt
     4321 caaaaaaacg gcaaaactcc aagaccctgc tatgcgcaac ctgcaagaac aggatgccgt
     4381 ctacatcgcc aagcagaaac aggcaaaagc ctccccgttc aaaaccgaaa tcgaaaccgc
     4441 cttggaagaa agcggcatta tcggcaactc cgcccacacc gtttccgaac cccaaaccgg
     4501 acattccgca ccgaaacctg ccgacgcgcc ggcaaaacct gcacccgttc cgcaaacacc
     4561 tgcaaaaccg ctgattacgc tcaaagaact gtcaaaagtc gaactgccgt ggtttgacgt
     4621 gcgcttcgac ttcatctcct atatcgcgct gaccgaagcc aaagaactgc acgcactgcc
     4681 gcgcctttcc aaccgctgcc gctaccagat tgtcggctgc accatggacg accatttcca
     4741 gattgccgaa cccatcccgg gcatccgcta tcaggcattt atcgtgggta ttcaggcagt
     4801 cagccgcaac ggacttgcct cgcaggaaga actctccgca ttcaaccgcc aggtggacgc
     4861 attcgcacaa agcatgggcg gtcagacgct gcacaccgac cttgccgcct ttatcgaagt
     4921 ggcttccgca ctggacgcat tctgcgcgcg cgtcgaccag accatcgcca tccatttggt
     4981 ttccccgacc agcatcagcg gcgtagaact gcgttccgcc gtaacgggcg tgggtttcgt
     5041 tttggaagac gacggcgcgt tccactatac cgacacgtcg ggctcgacca tgttctccat
     5101 ctgctcgctc aacaacgagc cgtttaccaa cgcccttttg gacaaccagt cctacaaagg
     5161 cttcagtatg ctgctcgaca tcccgcactc tccggcaggc gaaaaaacct tcgacgattt
     5221 gtttatggat ttggcggtac gcctgtccgg ccagttgaac ctgaatctgg tcaacgacaa
     5281 aatggaagaa gtttcgaccc aatggctcaa agacgtgcgc acttatgtat tggcgcgtca
     5341 gtccgagatg ctcaaagtcg gtatcgaacc gggcggcaaa accgcattgc gcctgttctc
     5401 ctaaaatcga atgcaatcaa agcggatatg tcgttacggc atatccgctt tttttcgggt
     5461 aaaattcagc tgttttcaac ctatcatttt tcagacgacc tttgcccgtt tcatcccgta
     5521 tcagacaaag gtcgtctgaa aaccttctct catgaatcca aactcaaaac ataacactaa
     5581 ctttaccaat cttcttaaac cgtctgatag tgatataaaa caatttgccg cacaacacat
     5641 ccgccacctc accgacctgc tcaaccgcta tgcctacgaa tactacaccc tcgacgcgcc
     5701 cagcgtaccc gatgccgaat acgacaaatt attccgcgag ctcgaagcgt tggaacgaaa
     5761 ccatcccgag ctcaaactgc ccgacagtcc gacccagcgc gtcggcggcg agcctttagc
     5821 gggatttgcc gaagtacgcc acgaagtgcc gatgctgtcg ctgaccaacg ctttttcccc
     5881 gcaagatgaa aacggcgtgt tcgaccatgc cgaaatgtac gctttcgacc aacgcgtgcg
     5941 cgacggtttg gacggcggca atcccgaata cgttatcgaa cctaaattcg acggcctcgc
     6001 catcagcctg ctctatcgcg acggcgtatt gattcaagca gcaacgcgcg gcgacggcac
     6061 gacgggcgaa gacgttaccc aaaacatcaa aaccgtatcc aacatcccct tgcggctgca
     6121 cggcgaaaat acgcccgaac tcatcgaagt acgcggcgaa gtactgatgc aaaaagccga
     6181 ttttgccgcc ctcaataaaa gacaagccga aaacgggcaa aaaccctttg ccaatccgcg
     6241 caacgccgcc gccggcagcc tacgccaact cgattcgcgc atcaccgcgc agcgcaaact
     6301 gcactttttc ccctactccg ttgcccgcca gcaaggcggt ttcgtcgcgg aagaacacat
     6361 ccaagaactc gcctatttcc aagcactcgg cttcagtctg cccaatggca atttcggctg
     6421 tttcaaaaat atcgacgaag tattggcgtt ttacggacac atgcagcaaa aacgccccga
     6481 actgccctac gaaatcgacg gcatggtcgt caaagtcaac agcttggcgc aacagcacga
     6541 actcggcttc atctcccgcg cgccgcgttg ggcggttgcc cacaaattcc ctgccgaaga
     6601 agccctgacc attgtcgaag cgattgacgt gcaaatcggc aggacgggcg cggtaacgcc
     6661 ggttgcccga ttacaacccg tattcgtcgg cggcgtaacc gttaccaacg ccaccctgca
     6721 caatcaggac gaagtatcgc gtaaagacgt gcgcgtcggc gataccgtcg tcgtgcgccg
     6781 cgctggagac gtgattcccg aagtcgtgcg tgtgattttt gaacgccgcc cgatgcagga
     6841 aaccgccgtt gctgtttcag acggcatcgg gcatcggcag gacgatttgt ttgccgaagc
     6901 accggctgca aaccaaaccc aatccgttcc gctctacaag ccctaccgcc tgccgaccca
     6961 ttgccccatc tgccgcagcg aaatcgaacg cgaagaaggt gaagccgtcg cccgatgcag
     7021 cggcggcatg ctttgtcagg cacaacgcgc gcaaggctta atccacttcg cctcgcgcaa
     7081 agcgatggac atcgacggct tgggtgaaaa acaaatcgaa cagcttgtcg cccaagacct
     7141 cgtccgccac ttcgccgacc tctaccgcct cgatattccg accctgcaaa aaatgaagga
     7201 aacggcggac aaaaccgttg ccgaatcgga tcaaatgccg tctgaaggca gttcggtcgg
     7261 cgcgtcgggc aaacataaaa aacaaccgac caagtgggcg gaaaacatcc tcgcaggcat
     7321 agaatccggc aaaacgcccg aactcgcgcg cttcctgttc gcgctcggca tccgccacgt
     7381 cggcgaacgc accgccaaaa cgctggcaca ggcattcggc acattggaac gcgtccgccg
     7441 cgcccccgaa cccgtcctcg cctgcctgcc cgacatcggt acggtagtgg cgcgttccat
     7501 tgcccacttc ttcgcccaag ccgaacagca ggcgatgata gacgagctgc tcgccgcagg
     7561 cgttgccccg caagcccaag ccgtcagcct gcctgccgca caatatgccg aaccgcaacg
     7621 ctggattgcc cgcctgcccg gtttcaaaat tagcgaaaac aaagcccaag ccttatggga
     7681 actcgccggc aaaagtatag agggtctgca aaccgacaaa tccctccccg ccgactggca
     7741 agcatggcgc agcgaaccac aaaacgccgc cctactcgaa aacctgaaaa ccttcttcgc
     7801 gcaaatgccg tctgaagacg aggcggcgca aggttcagac ggcatcaacg aagccgtagc
     7861 aggcaaaacc tttgtattaa ccggcaccct gcccacgctc aaacgcgacc aagcccaatc
     7921 cctaatcgaa gccgcaggcg gcaaagtttc cggcagcgtg tccaaaaaaa ccgactacgt
     7981 cgtcgccgga gaagccgcgg gcagcaagct ggaaaaagcc aatgccttgg gcgtttccgt
     8041 cctcagcgaa gccgaactgc ttaccctgct cggctgaacc tatgccgtct gaaccgcctt
     8101 cagacggcat tgtccacttt tccgaaccac accatgcaca ccatttcctt tccaaaccgc
     8161 acccgactca ccgccctgcc gcccctgtcg ctttacatcc acatcccgtg gtgcatcaaa
     8221 aaatgcccgt attgcgactt caattcccac agcctgaaaa acggattgcc cgaagccgcc
     8281 tatatcgacg cgctgctgac cgatttgcag cttgaattgc ccaatatttg gggcaggccg
     8341 gtggaaacga tctttttcgg cggcggtacg cccagcctgt ttcaggcgga atcgattgac
     8401 cgtttgttaa gcggcgtgcg ttcgctgttg cgcttgcagc cggaagcgga aattaccttg
     8461 gaagccaatc cggggacatt tgaaatcgag aagtttcaag gatttaaaga cgcgggtatt
     8521 acgcggcttt ctatcggcgt acagagtttc aacgacgata tgctctcaag gctggggcgc
     8581 gtccacaacg gtaaggaagc cttaaccgct atcgataccg ccttgaaatt atttgataaa
     8641 gtcaatatcg acttgatgta tgccctgccg aaccaaaccg ttcagacggc attggacgac
     8701 gtacaaaccg ctatcgctac gggcgcgacc cacatcagcg cgtatcatct gacgatggaa
     8761 ccgaacacgc cgttcggtca tacgccgccg aaaggtttgc cgcaagacga agccgccctc
     8821 gacatcgaag acgcggtaca cggcgcgctg gaaggcgcgg gttttgtcca ctacgaaaca
     8881 tcggcttttg cgaaaccagc catgcagtgc cgccacaatt tgaactactg gcagttcggc
     8941 gattatttag gcataggcgc gggcgcgcac ggcaaaattt cctatcccga ccgcatcgag
     9001 cgcaccgtcc gccgccgcca ccccaacgac tacctcgcct taatgcaaag ccaaccgagt
     9061 gaagccgtcg aacgcaaaac cgtcgctgcc gaagatttgc cgttcgaatt catgatgaac
     9121 gccctgcgcc tgaccgacgg cgtacccgcc gcgatgttgc aggagcgcac gggcgtaccg
     9181 agtgccaaaa tcatggcgca aatcgaaacg gcaaggcaaa aaggcctgct ggaaaccgac
     9241 cctgccgtat tccgcccgac cgaaaaagga cgcttgtttt taaacgattt gctgcagtg
//
LOCUS       DAXHKX010000068         9271 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHKX010000068 DAXHKX010000000
VERSION     DAXHKX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.068"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            168..1511
                     /locus_tag="ACFMWZ_001204"
     CDS             168..1511
                     /locus_tag="ACFMWZ_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC5935169.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     DRYAPDALAKLSGKEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTGRVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LLGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1514..2746
                     /locus_tag="ACFMWZ_001205"
     CDS             1514..2746
                     /locus_tag="ACFMWZ_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC5935170.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2739..3515
                     /locus_tag="ACFMWZ_001206"
     CDS             2739..3515
                     /locus_tag="ACFMWZ_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC5935171.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3515..4141
                     /locus_tag="ACFMWZ_001207"
     CDS             3515..4141
                     /locus_tag="ACFMWZ_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC5935172.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4145..4738
                     /gene="nqrE"
                     /locus_tag="ACFMWZ_001208"
     CDS             4145..4738
                     /gene="nqrE"
                     /locus_tag="ACFMWZ_001208"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC5935173.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4752..5969
                     /gene="nqrF"
                     /locus_tag="ACFMWZ_001209"
     CDS             4752..5969
                     /gene="nqrF"
                     /locus_tag="ACFMWZ_001209"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC5935174.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6121..7176
                     /locus_tag="ACFMWZ_001210"
     CDS             6121..7176
                     /locus_tag="ACFMWZ_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC5935175.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     TGGYRTAMSSEFEKLLR"
     gene            7202..7417
                     /gene="nqrM"
                     /locus_tag="ACFMWZ_001211"
     CDS             7202..7417
                     /gene="nqrM"
                     /locus_tag="ACFMWZ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC5935176.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7541..8119)
                     /gene="grpE"
                     /locus_tag="ACFMWZ_001212"
     CDS             complement(7541..8119)
                     /gene="grpE"
                     /locus_tag="ACFMWZ_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC5935177.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8303..9121
                     /gene="cysE"
                     /locus_tag="ACFMWZ_001213"
     CDS             8303..9121
                     /gene="cysE"
                     /locus_tag="ACFMWZ_001213"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC5935178.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2230 a         2615 c         2300 g         2126 t
ORIGIN      
        1 taaaaacggc ttccgcaagg aaaaaagcag caaatctgac aaacaggtac aattcctgat
       61 agaatctaac actttgctac attaatttat tcaatgcttt tgatgccacg cgacacggta
      121 tcaaaacacg ccgtttttac cttaaccatc acaagtaatg caaatatatg attaaaatca
      181 aaaaaggtct aaacctgccc atcgcgggca gaccggagca agtcatttat gacggcccgg
      241 ccattaccga agtcgcgttg cttggcgaag aatatgccgg tatgcgcccc tcgatgaaag
      301 tcaaggaagg cgatgccgtc aaaaaaggcc aagtgctgtt tgaagacaaa aagaatccgg
      361 gcgtggtgtt taccgcgccg gcttcaggca aaatcgccgc catccatcgc ggcgaaaagc
      421 gcgtacttca gtcggtcgtg attgccgttg aaggcaacga cgaaatcgaa ttcgaccgct
      481 acgcgcccga tgccttggca aaattgagcg gcaaagaagt gcgccgcaat ctgattcaat
      541 ccggtttgtg gactgcgctg cgtacccgtc cgttcagcaa aatccctgcc gtcgatgccg
      601 agccgttcgc catcttcgtc aatgcgatgg acaccaatcc gctggcggca gaccctgtgg
      661 ttgtgatcaa agaagccgcc gaggatttca gacgaggttt gctggtattg agccgtttga
      721 ccgagcgtaa aatccatgtg tgtaaagcag caggcgcaga cgtgccgtct gaaaacgccg
      781 ccaacatcga aacacatgaa ttcggcggcc cgcatccggc cggtttgagt ggcacgcaca
      841 ttcatttcat tgagccggtc ggtgcaaaca aaaccgtttg gaccatcaat tatcaagatg
      901 taattgccat cggacgtttg tttgcaacag gccgtctgaa caccgggcgc gtggttgcct
      961 tgggtggttc tcaagtcaac aaaccacgcc tcttgcgtac cgttttgggt gcgaaagtat
     1021 cgcaaattac tgcgggcgaa ttggttgacg cagacaaccg cgtgatttcc ggttcggtat
     1081 tgaacggcgc gattgcacaa ggcgcgcacg attatttggg acgctaccac aatcagattt
     1141 ccgttatcga agaaggccgc agcaaagagc tgctcggctg ggttgcgccg cagccggaca
     1201 aatactccat cacgcgtaca accctcggcc atttcctgaa aaacaaactc ttcaagttca
     1261 acacagccgt caacggcggc gaccgcgcca tggtgccgat tggtacttac gagcgcgtga
     1321 tgcccttgga tatcctgccc accctgcttt tgcgcgattt aatcgtcggc gataccgaca
     1381 gcgcgcaggc attgggttgc ttggaattgg acgaagaaga cctcgctttg tgcagcttcg
     1441 tctgcccggg caaatacgaa tacggcccgc tgttgcgcaa agtgctggaa accattgaga
     1501 aggaaggctg attatgggct tgaaacattt tctggaaaaa atcgaaccgc acttcctgcc
     1561 gggcggcaaa catgaaaaat ggtatgccct ctacgaagcg gcggcgacga ttttctacac
     1621 atccggggcg gtaacgcgca aagcggcaca cgtccgcgac gcgctcgact ccaagcgcat
     1681 gatgattttg gtgtggctgg ctttgttccc cgccatgttt tacggcatgt acaacgtcgg
     1741 cgcacaggca ttcggtgcct taacgcccga tttgctgcaa caaagcatcg ccaacgactg
     1801 gcattacacc cttgccgacg ctttgggcat caatatgtcg tctgaagcgg gcgtgttggg
     1861 caaaatgctg ttcggcgcga tttacttcct gccgatttac gcgaccgtat ttgccgtcgg
     1921 cggtttctgg gaagtcttgt tcgccaccgt acgcaaacac gaaatcaacg aaggtttctt
     1981 tgttacctcg attctgtttg ccttaatcgt tccgcccacg ctgccgctgt ggcaggcggc
     2041 tttgggtatt tctttcggcg ttgtggttgc gaaagaagta ttcggcggta cgggtaaaaa
     2101 ctttatgaac cctgcgctgg caggccgcgc cttcctgttc ttcgcctacc ccgccaactt
     2161 gagcggcgat gcggtttgga cggcggttga cggctattcc ggcgcaaccg cgctggcgca
     2221 atgggcggca cacggtgcag acggcctgaa aaacgccgta accggtcaaa ccatcacttg
     2281 gatggacgcg tttatcggca aactgcccgg ctccatcggc gaagtctcca ctttggcact
     2341 cttaatcggc ggcgcgttta tcgtgtttgc ccgcatcgct tcttggcgca ttattgccgg
     2401 cgtgatgatc ggtatgattg cgatgtcttc gctgttcaac ttcatcggtt cggacaccaa
     2461 cgctatgttt gccatgcctt ggtactggca tttggtcgtc ggcggcttcg ccatcggtat
     2521 gctgtttatg gcgaccgacc ctgtttccgc ttcctttacc aatgtcggca aatggtggta
     2581 cggcgcgctg atcggcgtga tgtgcgtgtt gatccgcgtg gtcaatccgg cttaccccga
     2641 aggtatgatg ttggcgattc tgtttgccaa cctgtttgcc ccgattttcg actatttcgt
     2701 cgcacaagcg aacatcaaac gcagaaaggc gcgcagcaat ggctaagaaa ttcgataaag
     2761 acagcttcag cggcacgctg attgtcgtgt tggcggtcag cctgatttgc tcggtcatcg
     2821 ttgccggtgc ggtcgtcggc ttgaaaccca tccaagagaa acaaaaactc caagacaaac
     2881 aaggctatat cttgagcgta gccggtttga tggataagga caccgacatc ggcaaaacct
     2941 ttgccgagcg tatcgagcaa cgtgttgtcg atttggcgac cggcgaatac gtcgccgatg
     3001 cgcctaaaga cttcagcgcg cgcatcgcag gcaaagaccc tgcccaaagc atccgcatca
     3061 aacccgaaga cgatttggca ggcatcaaaa gccgtgccaa atacaccgag gtttatttgg
     3121 taaaaggcga agacggcaaa atcgggcaaa tcatcctgcc tatgcacggt aacggtttgt
     3181 ggtcggtcat gtacggcttt gtcgccatcc agcccgacgg caacaccatc aacggcatta
     3241 cctactacga acaaggcgaa accccgggct tgggcggcga aatcggcaat ccgttgtggc
     3301 agcaaaaatt cgtcggcaaa aaactgtttg acggacaagg caaactcgcc ctgcacgtcg
     3361 gcaaaggcgc gggttcggac aaagaacacg gcgtagatgc cctctccggc gcatcgctga
     3421 catccaaagg cgtgcaaggt tcgttcgcct actggttcgg cgaaaacggc tatatcccct
     3481 acctgaacaa attgaaatca gcaggagcgc aataatggct gatatgaaac gcttgaaaca
     3541 tttgatgttt tcacccttta tcgacaacaa cccgattgcc ttgcaggttt tgggtatttg
     3601 ttcggctttg gcggttacca ccaaacttca gacggccatc gtgatgggta tttccgtagc
     3661 tttggtaacc ggtttttcca gcttcttcat ttcgctggta cgcaactaca tccccaacag
     3721 catccgtatt atcgtgcaaa tggcgattat cgcgtcgctg gttacgctgg tcgaccaact
     3781 cttgcaggca tttgcctacg aattgtccaa acagctttcc gtattcgtcg gtctgattat
     3841 taccaactgt atcgtgatgg gccgcgccga agcatttgcg atgaaagagc cgccgctgga
     3901 aagcctgatc gacggcatcg gcaacggcgc gggctacggg ataatgctgc ttgtcgtcgc
     3961 caccgtccgc gaactgattg gctcgggcaa actcttgggc tacaccgttt tccaaaccgt
     4021 gcaggacggc ggctggtatc agaccaacgg cttgttcctg ctcgccccca gcgcgttctt
     4081 catcatcggc tttttgattt ggggactgcg tacctggaaa cccgaacagg cggaggaata
     4141 agctatggaa cactatttga gcctcttcat caaatccgtc ttcattgaaa atatggcact
     4201 atccttcttt ttgggtatgt gcacgttttt ggcggtatcc aaaaaagtat ccaccgcatt
     4261 cggtttgggt gtggcggtaa ttttcgtact cgggctgtcc gtccctgtca accaactcgt
     4321 ttactcgctg ctcaaagacg gcgcgattgt cgaaggcgtg gatttgacct ttttgaaatt
     4381 catcaccttc atcggcgtga ttgccgcttt ggtgcagatt ttggaaatgt tcttggataa
     4441 attcgtcccc gccctctata acgcattggg catctacctg ccgctgatta ccgtaaactg
     4501 cgcgattttc ggcgccgtat cgtttatggc gcaacgcgaa tacaacttcg gcgagtccgt
     4561 tgtatacggc ttcggcgcgg gcttgggctg gatgttggcg attgtcgctt tggcgggcat
     4621 taccgaaaaa atgaaatatt cggacgctcc caaaggcctc aaaggcttgg gcattacctt
     4681 tatcgccgcc ggcctgatgg cgatggcgtt tatgtcgttc tctggcatcc agttataaga
     4741 aagggatcgg tatggagatt attttaggta tcgtgatgtt taccgtcatc gttttggttt
     4801 tggcactgat gattctgttt gccaaatcca aactggtcag cgaaggcgac atcaccatca
     4861 aagtcaacgg cgaaaaagag ctgacgatgc ccgccggcgg caaactcttg ggcgcgcttg
     4921 ccaacgaagg catctttatc ccctccgcct gcggcggggg cggttcgtgc ggacaatgcc
     4981 gcgtcgtcgt aaaaagcggc ggcggcgaca tcctgccgac cgagctgtcc cacatcagca
     5041 aacgcgaagc acgcgaaggc tgccgtctgt cgtgccaagt caacgtcaaa accgacatgg
     5101 acatcgaagt ccccgaagaa gtgttcggcg tgaaaaaatg ggaatgcacc gtcatctcca
     5161 acgataacaa agccacgttc attaaagaac tcaagcttgc cattcccgaa ggcgaagaag
     5221 tccccttccg cgccggcggc tacattcaaa ttgaagcccc gccgcacacc gttgcctaca
     5281 aagacttcga cattcccgag gaataccacg aagactggga caaatacaat ttgtggcaat
     5341 acgtttccaa agtggacgag ccgattttgc gtgcctactc tatggcttcg tatcctgaag
     5401 aaaaaggcat cattatgctg aacgtgcgta tcgccacgcc gcccccgcgc gtacctgatg
     5461 cgcctccggg acaaatgtcg tcctacatct ggtcgctcaa acccggcgac aaagttacga
     5521 tttccggccc gttcggcgaa tttttcgcca aagacaccga tgccgaaatg gtatttatcg
     5581 gcggcggtgc gggtatggct ccgatgcgtt cccacatttt cgaccagttg aaacgtttga
     5641 actccaaacg taagattacc ttctggtacg gcgcacgttc caaacgcgaa atgttctatg
     5701 tcgaagactt cgaccaactc gcagcagaat tcccgaactt cacatggcac gtcgccctgt
     5761 ccgacccatt gcctgaagac aactgggacg gctacacagg cttcattcac aacgtggttt
     5821 acgaaaacca tctgaaaaac cacgaagcgc cggaagactg cgaattttat atgtgtggtc
     5881 ctccgatcat gaaccagtcc gtcatcaaaa tgcttaaaga cttaggcgtg gaagacgaaa
     5941 acatcctctt ggacgatttc ggcggttaaa tattcggctt caaacacaaa aggaagaaac
     6001 ctcggtttct tcctttttct atccgatgcc gcccgaaccc ccttcagacg gcatcggcat
     6061 agaaacatat cggccgtttt gcgatataat tcaaaccatt tgccaaaacc gtcaaacact
     6121 atgccgtctg aaacacgcct gccgaacttt atccgcgtct tgatatttgc cctgggtttc
     6181 atcttcctga acgcctgttc ggaacaaacc gcgcaaaccg ttaccctgca aggcgaaacg
     6241 atgggcacga cctataccgt caaatacctt tcaaataatc gggacaaact cccctcacct
     6301 gccgaaatac aaaagcgcat cgatgacgcg cttaaagaag tcaaccggca gatgtccacc
     6361 tatcagcccg actccgaaat cagccggttc aaccaacaca cagccggcaa gcccctccgc
     6421 atttcaagcg acttcgcaca cgttactgcc gaagccgtcc gcctgaaccg cctgacacac
     6481 ggcgcgctgg acgtaaccgt cggccccttg gtcaaccttt ggggattcgg ccccgacaaa
     6541 tccgttaccc gtgaaccgtc gccggaacaa atcaaacaag cagcatctta tacgggcata
     6601 gacaaaatca ttttgaaaca aggcaaagat tacgcttcct tgagcaaaac ccaccccaag
     6661 gcctatttgg atttatcttc gattgccaaa ggcttcggcg ttgataaagt tgcgggcgaa
     6721 ctggaaaaat acggcattca aaattatctg gtcgaaatcg gcggcgagtt gcacggcaaa
     6781 ggcaaaaacg cgcgcggcga accttggcgc atcggcatcg aacagcccaa catcgtccaa
     6841 ggcggcaata cgcagattat cgtcccgctg aacaaccgtt cgcttgccac ttccggcgat
     6901 taccgtattt tccacgtcga taaaagcggc aaacgcctct cccatatcat taatccgaac
     6961 aacaaacgac ccatcagcca caacctcgcc tccatcagcg tggtcgcaga cagtgcgatg
     7021 acggcggacg gcttgtccac aggattattc gtattgggcg aaaccgaagc cttaaagctg
     7081 gcagagcgcg aaaaactcgc tgttttcctg attgtcaggg atacaggcgg ctaccgcacc
     7141 gccatgtctt ccgaatttga aaaactgctc cgctaaacat acaaaccgat taggaaacac
     7201 catgaaaacc ctgctcctca ccttcggcat cttcctgacc gtcatcatcg gtatggcggt
     7261 cggctatatc ttctccaaac gcaccatcaa aggcagttgc ggcggcatta ccgccttggg
     7321 tatgaaaaaa atgtgcgact gcgacacgcc ttgcgacacc ctgcaaaaga aactggatga
     7381 agaaaaacag gcaggcggca tacgggttga ccgttaacac cgaaacaaaa tgccgtctga
     7441 agcccgacag gcgggttcag acggcattgc cgctttactt cgatcggttt atacgcaaaa
     7501 cgcgcttcag gaaataaatc agattattcc ccagacgcct tcaggcttcc ttctgtgcca
     7561 ccgtaaccat agccgggcgc aacacgcggt cggacagcgt ataacccttc ttcatcacgc
     7621 ccaccacggt attgggttcc tgttcgcttg ccaccgcctg catcgcctga tggatattcg
     7681 gatcgagctt atcgcccgct ttaggattga tttccttgat ttgcgtagca tcaaatgctt
     7741 tctgcaactc gttcaaagtc atctgcacgc ccattttcag cgcatcgaaa ttgccgctct
     7801 gatccaaaag cgccatttcc agataatcct tgaccggcag catttccacg gcaaacttct
     7861 gtccggcgaa cttgtgcgta tccgcaattt cctgctggtg gcggcggcgc aggttttgct
     7921 cgtttgccaa agcgcgcagc tgctcgtctt tcaactgcgc ttccagctcg gcaatccgcg
     7981 cctgcaaatc ctcataagcc ggctctgcgg cagcctgttc ctgcacaccg tccgcatttc
     8041 ctactgtctc gacggtttcc accgcctcca cattttcaac cgcttcttca ctgttttgct
     8101 gctgtgtctg ttcgctcata tcgtatccct taaaacaaat tggaaatcaa aatccagtta
     8161 ttacccgcgc aagataagga cattttcaag aaacttcaag caaaagcaga gaatttcaaa
     8221 ttcattttca aatcccctac cgaaaaaata atatagacgg taagatactg ccgaccatat
     8281 cgattttcaa acgtcatcac tcatgaaaaa agaccattta aacacaaccg gtttcgacct
     8341 ctggcacacc atccgcgaag aaaccgcggc cgcggtctcc gccgaaccga tgctggcaag
     8401 ttttttgcac caaaccgtgt tgcgccacga gtccctcggc tccgtccttg cctaccacct
     8461 ttccagcaaa ctcggcagcc cgattatgga cgtgcgcgcg ctgtttgaaa tctaccagca
     8521 ggcgttgggc agcgacaccc aaatcggcaa atgcgtcgaa gcagacttaa aagccatcta
     8581 cgaacgcgat cccgcctgcg acgaatattc gctgccgctt ttatatttca aaggcttcca
     8641 cgcgattcag gcacaccgca tcaaccatcg gctgtatctc gacgggcgca aaacgctggc
     8701 gtatttcctg caaaaccgta tgtccgaagt attcggcgtg gacatccatc ccgccgcccg
     8761 tttcggatac gggctgatgc tcgaccacgc caccggcttt gttgccggcg aaaccgccgt
     8821 gttgggcaac aatatttcga ttttgcacgg cgtaacgctc ggcggttcgg gcaaagaagg
     8881 cggcgaccgc caccctaaaa tcggcgacgg cgtgatgatc ggcgcaaacg cctcgatatt
     8941 gggcaatatc cgcatcggca gcaatgccaa aatcggggcg ggcagcgtcg tggtttcaga
     9001 cgtgccgccg tccatcacgg ttgtcggcgt acccgccaaa cccgtggcgc gatcgctcaa
     9061 aacaccgtcg gcggatatgg atcaaaatat ccagtttacc gaaatcgact ttatgatttg
     9121 aatatccgca ccaatgccgt ctgaaacgcc aaacgggctt cagacgacat tgcccgcttc
     9181 aagcctgcgc cttgatatgc cgccgatgtc ggcacttgcc agtcggacaa aatgcctttt
     9241 attcaataca cccgattccc acgcaaccgg a
//
LOCUS       DAXHKX010000069         9071 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHKX010000069 DAXHKX010000000
VERSION     DAXHKX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.069"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(129..464)
                     /locus_tag="ACFMWZ_001214"
     CDS             complement(129..464)
                     /locus_tag="ACFMWZ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC5935179.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            593..1498
                     /gene="mtrA"
                     /locus_tag="ACFMWZ_001215"
     CDS             593..1498
                     /gene="mtrA"
                     /locus_tag="ACFMWZ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC5935180.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1560..2048)
                     /locus_tag="ACFMWZ_001216"
     CDS             complement(1560..2048)
                     /locus_tag="ACFMWZ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC5935181.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2195..3097
                     /locus_tag="ACFMWZ_001217"
     CDS             2195..3097
                     /locus_tag="ACFMWZ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC5935182.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3138..4268)
                     /locus_tag="ACFMWZ_001218"
     CDS             complement(3138..4268)
                     /locus_tag="ACFMWZ_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC5935183.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4473..7097
                     /gene="alaS"
                     /locus_tag="ACFMWZ_001219"
     CDS             4473..7097
                     /gene="alaS"
                     /locus_tag="ACFMWZ_001219"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC5935184.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADMARELGIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGMRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7159..7890
                     /locus_tag="ACFMWZ_001220"
     CDS             7159..7890
                     /locus_tag="ACFMWZ_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935185.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8317..>9071
                     /locus_tag="ACFMWZ_001221"
     CDS             8317..>9071
                     /locus_tag="ACFMWZ_001221"
                     /inference="COORDINATES: protein motif:HMM:NF042870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFC5935186.1"
                     /translation="MDRKNVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTK
                     AKEQAERYLQEIGRTKPSALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYI
                     TRGVFDFMFGIEAKVRQIQEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFL
                     FFADDSAVFQRNYLFQDFLESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFE
                     YVNGGLFKERLRTFDFTAKQHRALIDCGNFDWR"
BASE COUNT         2310 a         2387 c         2186 g         2188 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aaaaacggct gcatatgttg tctacaaaat
       61 atcgggaagc ataacaggca aaatgccgtc tgaaatattt tcagacggca tttcttatcc
      121 gaaacggatt atttttgcgt ttcaaccgct tccaatgcac gcagggcata agtgtaagcg
      181 gcaccggcat tcagggcgat ggcggttgcc aatgcgcctg cgatttcgct gtcggtcgca
      241 ccggctttgg cggcagcggc ggcgtgaacg ctgatgcagc tctcgcaacg agtggtaatg
      301 gcaacggcga tggcaatcag ttcgcgcgtt ttggcatcaa gtgcttcggc ggcggcggcc
      361 tgttccaatg cgccgtaggc ctgcagcatt ttaggatgcg ccttacccag ctcgccgaac
      421 gattttttaa ccaatgcggt atgttctttc caatctttaa acattttctt ttcctttctc
      481 ttgcgtttaa ccctgatacg cgcttgcgta tctgttttcg atgtgcgtat tattgcaatt
      541 attcagttgt gtttctcgtt taatcatctc attttatggt tcaaaaagat ttatggacat
      601 tctggacaaa ctggtcgatt tcgcccaatt gacgggcagt gtggatgtgc agtgcctttt
      661 gggcggacaa tggtcggtac ggcatgaaac cttgcaacgc gaaggattgg tacacattgt
      721 tacatcgggc agcggctatc tctgcatcga cggcgaaact tccccgcgtc cggtcagtac
      781 aggggatatt gtatttttcc cgcgcggctt gggtcatgtg ttgagccacg acggaaaatg
      841 cggagaaagt ttacaaccgg atatgcggca gcacggtgcg tttacggtca agcagtgcgg
      901 caacggacaa gatatgagcc tgttttgcgc ccgtttccgc tacgacaccc acgccgattt
      961 gatgaacggg ctgcctgaaa ccgtttttct gaacattgcc catccgagtt tacagtatgt
     1021 ggtttcaatg ctgcaactgg aaagcaaaaa acctttgacg gggacggttt ccgtggtcaa
     1081 cgcattgtcg tccgtcctgc tggtgcttat cctgcgcgcc tatctcgaac aggataagga
     1141 tgtcgaactc tcgggcgtat tgaaaggttg gcaggacaaa cgtttgggac atttaatcca
     1201 aaaggtgata gacaaaccgg aagacgaatg gaatgtcgac aaaatggtgg cggctgccaa
     1261 tatgtcgcgc gcgcaactga tgcgccgttt caaaagccgg gtcggactca gcccgcacgc
     1321 ctttgtgaac catatccgcc tgcaaaaagg cgcgttgctg ctgaaaaaaa acccggattc
     1381 ggttttgtcg gtcgcactgt cggtaggctt tcagtcggaa acgcacttcg gcaaggcgtt
     1441 caaacggcaa tatcacgttt cgccgggtca ataccggaaa gaaggcgggc aaaaataaat
     1501 cggggcttca aacgcaaatg ccgtctgaaa aggctttcat acagcatttg cgtaccgcgt
     1561 catttcaagg gctgcatctt catcacttcc atcaaaaagt tggtaaatgc ggggttgttg
     1621 ggtttgacat ccatattttt ccaacgctgc tgccagccgc gcaaggcatt ctggatatac
     1681 agcttggact gttccgtatt gattgcgccc cgctggctgt ctatcgccga acgcaggtag
     1741 atttcataca tactgtcatc gacggcattg cgtccgacca ggcgttttct gaagttgttc
     1801 agatattgcg ccgcctgaac cttggtcatt ttaccgatac ccacctgata gcccaagcgc
     1861 gtcgcttcat cgctgatttt ggcaacatcc gtccaatgcg aagaggcaag gcggaaacct
     1921 tttgcaggtg cttccgtttt gacggtattg ataggattca cggggatttc cgtcaatgtg
     1981 ggcacataaa tagactggca gccggaaaga actgccacaa tggaaagagg gataaggtat
     2041 tttttcatgc ccccattata atcaagtttg ccttgagaaa acaaattgtt cggcaagaaa
     2101 aataaaattt cggcatcaga agcaggcaaa aacacattcc acaagccttg ccgcaaggtt
     2161 tacaatccga ccgtccttat cgcaacgacc gtttatggat accgcaaaaa aagacatttt
     2221 aggatcgggc tggatgctgg tggcggcggc ctgctttacc attatgaacg tattgattaa
     2281 agaggcatcg gcaaaatttg ccctcggcag cggcgaattg gtcttttggc gcatgctgtt
     2341 ttcaaccgtt gcgctcgggg ctgccgccgt attgcgtcgg gacaccttcc gcacgcccca
     2401 ttggaaaaac cacttaaacc gcagtatggt cgggacgggg gcgatgctgc tgctgtttta
     2461 cgcggtaacg catctgcctt tggccaccgg cgttaccctg agttacacct cgtcgatttt
     2521 tttggcggta ttttccttcc tgattttgaa agaacggatt tccgtttaca cgcaggcggt
     2581 gctgctcctt ggttttgccg gcgtggtatt gctgcttaat ccctcgttcc gcagcggtca
     2641 ggaaacggcg gcactcgccg ggctggcggg cggcgcgatg tccggctggg cgtatttgaa
     2701 agtgcgcgaa ctgtctttgg cgggcgaacc ctgctggcgc gtcgtgtttt acctttccgt
     2761 gacaggtgtg gcgatgtcgt cggtttgggc gacgctgacc ggctggcaca ccctgtcctt
     2821 tccatcggca gtttatctgt catgcatcgg cgtgtccgcg ctgattgccc aactgtcgat
     2881 gacgcgcgcc tacaaagtcg gcgacaaatt cacggttgcc tcgctttcct atatgaccgt
     2941 cgttttttcc gccctgtctg ccgcattttt tctgggcgaa gagcttttct ggcaggaaat
     3001 actcggtatg tgcatcatca tcctcagcgg cattttgagc agcatccgcc ccactgcctt
     3061 caaacagcgg ctgcaatccc tgttccgcca aagataaaaa atgccgtccg aacatccttc
     3121 agacggcata tcgggcttta tttccccgcc ttcacatcct gccactggcg caccataaac
     3181 ttcaatgccg ccggctggat aggcaccatg ataaagctgt ttttcaaatc ctcctcggtt
     3241 gggaaaatcg tattgtcgtt tttaaattcg tcttccatca gctcacgcgc cggcttgctc
     3301 gaaggcgcat aagtaacgaa attgccgttt ttcgccgaca cttccgggtc gaggaagtcg
     3361 ttgatgtatt tgtgcgcgtt ggcgacgttt ttcgcatctt tcggaatcac gaaagaatcc
     3421 acccaaatcc ccacgccctc tttgggcatc atcacgcgga ttttttcctt gccgcccgct
     3481 tcttcggcac ggcgtttggc gatgttcaaa tcgccgccga aaccgattgt tacgcaggta
     3541 tcgccgcgcg ccaaatcatc gataaagccg gacgaagtaa agcgtttgat attggggcgg
     3601 tttttcttga gcagggcggt tgcctccctg atgtcttccg tattgctgct gttcgggttt
     3661 ttacccaaat agttcaacac cataggatag atttccgccg cgctgtctaa ataactgata
     3721 ccgcattgtt tgagtttgga cgtgtattcg gggtcgaaca ccaaatccca ctggttgtcc
     3781 ggcagcttgt ccgtacccaa agcctttttc acgcgttcgg tattgatggc gaaggtattt
     3841 gtcccccaat aaaacggcac ggcgtattcg tggccgggat cgaccccgtc catcagcctc
     3901 atcatttcgg gattgaggtg tttataattg ggaatcagcg atttatcgat tttctgatac
     3961 gcacctgcct taatctgcct gcccacaaac gcattggacg gcgcgacaat gtcgtaaccg
     4021 gattttccgg tcagcacctt gctttccagc gtttcatcgc tgtcgtacac atcataagta
     4081 accttgatgc cgtttttctt ttcaaaatcg gcaacggttt ccggatcgac gtattccgac
     4141 cagttgtaaa ttttcaatac gtttcggttt tccgccggcg ccggtttttc ggcaggcggt
     4201 ttgtccgaac cgccgcacgc tgcaagcagc aaagcagtca ggacggcgag tggcagatgt
     4261 ttggccatta tcattccttg catatcgggt tggagaaagc ggtcattata gccgatattg
     4321 gcaacagggc ttcagacggc attcaaaatc ccgccacacc cttccgaaaa ccgccgcttc
     4381 catagctaga aacagggatt tgcggtaaga taccgccgtt cgttttccct gcttttacca
     4441 tgacaagaca tttgagagac attgaaaaaa ttatgaaaac ctccgaactg cgccaaaaat
     4501 tcctaaaatt ttttgaaacc aaaggccaca ccgtcgtccg ctcttccagc ctcgtgccgc
     4561 acgacgaccc gaccctgctg tttaccaacg cgggcatgaa ccagtttaaa gacgtattct
     4621 taggtttcga caaacgcccg tacagccgcg ccaccaccgc gcaaaaatgc gtacgcgcag
     4681 gcggcaagca caacgacttg gaaaacgtcg gctacaccgc ccgccaccac accttctttg
     4741 aaatgatggg caacttctcc ttcggcgact acttcaagcg cgacgcgatc cacttcgctt
     4801 gggaatttct gacttccccc gaatggctca atatccccaa agacaaactg ttggcgaccg
     4861 tttacgcgga agacgacgaa gcctacaaca tctggttgaa cgaaatcggt atgccgtccg
     4921 agcgcatcgt ccgcatcggc gacaacaaag gcgcgaaata cgcgtccgac aacttctggc
     4981 aaatgggcga caccggccct tgcggcccct gctccgaaat tttctacgac cacggcgaag
     5041 aaatttgggg cggcattccc ggcagccccg aagaagacgg cgaccgctgg atcgaaattt
     5101 ggaactgcgt attcatgcag ttcaaccgcg acgaacaagg caatatgaat ccgctgccca
     5161 agccgtccgt cgataccggc atgggcttgg aacgcatggc cgccgttatg cagcatgttc
     5221 acagcaacta cgaaatcgac ttgttccaag acctgctcaa agccgttgcc cgcgaaaccg
     5281 gcgcagcttt cagcatggaa gaaccaagcc taaaagtcat cgccgaccac atccgctcct
     5341 gctcattcct gattgcagac ggcgtattgc cttccaacga aggccgcggc tacgtattgc
     5401 gccgcattat ccgccgcgcc gtgcgccacg gttacaaact gggtcaaagc aaaccgttct
     5461 tccacaaact cgttgccgat ttggtcaaag agatgggcgg tgcctaccct gagttgaaag
     5521 agaaacaagc acaaatcgaa gaagcgttga aaaacgaaga aagccgtttt gcccaaacgc
     5581 tggaaactgg tatggctttg ttggaaaacg cgctggccaa aggcggcaaa acactcgacg
     5641 gcgaaatcat cttcaaactc tacgatacct acggtttccc ctacgacctg actgccgaca
     5701 tggcgcgcga actgggcatc gaactggacg aagcaggctt cgagcgcgaa atggaagccc
     5761 aacgcgcacg cgcacgcgcc gcccaaagct tcaaagccaa cgcccaactg ccttatgacg
     5821 gtcaagacac cgagtttaaa ggttatagcg aacgccaaac cgaatccaaa gtcctcgccc
     5881 tctacaaaga cggcgagcaa gtcgacgaat tgaacgaagg cgacagcggc gcagtcgtca
     5941 tcgactttac cccgttctat gcagaatccg gcggccaagt cggcgatgtc ggctatatct
     6001 tctcaggcga aaaccgcttt gaagtacgcg atacccaaaa aatcaaagcg gccgtattcg
     6061 gtcaattcgg cgtacaaact tcaggccgtc tgaaagtcgg cgacagcgtt accgccaaag
     6121 tggacgacga aatccgcaat gccaatatgc gcaaccacag cgcaacccac ttgatgcaca
     6181 aagccctgcg cgatgtattg ggcagacacg tcgaacaaaa aggctctttg gttaccgccg
     6241 aatccacccg tttcgacatt tcccatcccc aagcggtaac tgccgaagaa atcgccgaag
     6301 tcgaacgccg cgtcaacgaa gccgttttgg cgaacgttgc cgtcaacgcc gccattatga
     6361 gtatggaaga cgcacaaaaa accggcgcga tgatgctctt cggtgaaaaa tacggcgacg
     6421 aagtgcgcgt actgcaaatg ggcggtttct ctaccgaatt gtgcggcggc acacacgttt
     6481 cacgcaccgg cgacatcggc ctcttcaaaa tcatcagcga aggcggtatt gccgcaggca
     6541 tgcgccgtat cgaagccatc accggcctga acgcactcaa atgggcgcaa gagcaagagc
     6601 gtttggtgaa agacatcatt gctgaaacca aagcccaaac cgaaaaagac gtactggcga
     6661 aaatccaagc aggcgcggca cacgccaaag cattggaaaa agaattggca cgcgccaaag
     6721 ccgaactcgc cgtccacgca ggcgcaaaac tcttggacga cgcaaaagac ttgggcgcag
     6781 ccaaactcgt tgccgcccaa atcgaagccg acgcagccgc cctgcgcgaa atcgttaccg
     6841 atttaaccgg taaatccgac aacgccgtga ttcttttagc ggcagtaaac gacggcaaag
     6901 tctccctgtg cgccggcgta tccaaaccgt tgaccggcaa agtgaaagca ggcgatctgg
     6961 ttaaatttgc agccgaacaa gtcggcggca aaggaggcgg ccgtcccgac ttggcgcagg
     7021 ccggcggcac ggatgccggc aaattgcccg aaatgctggt aagcgtggaa agctggcttt
     7081 gtcagaaatt aagttaaatt taaatcttaa taagccattt gagtcttgtt caaatggctt
     7141 atattttgga ttgttagaat gaatactaaa acagagttac aaaaactttt agaagaggat
     7201 atatcaactt taacagaaac actaatttgt gcagatgctc ttcctccaag atatgtacgt
     7261 agcattgcaa ctcctattgt ccgtcgatgg ttaattgaca aacaattaaa tattctcgca
     7321 aaagaaatag gtttaactat agaattgccc atccttgata caagtttggt atttgaaaaa
     7381 ttgtcaacct tagagaataa agttaatttt tatatggctg gtggagttta tttaggcgga
     7441 gaatttatta gctctatata tcattcatca caagaatttt ctggtgaacc aataatctat
     7501 gctgaaccaa atataatttt atgtcctgct gaaaaatttt taactttaaa acgagttttc
     7561 cataatggaa atatttttaa tatgaatcag ataattactt ttttatccaa taaacaagga
     7621 ggagtacatt ttgataaaaa ttacgataaa tataaaactt ggcaagttgc tatagaaaaa
     7681 gcagcaaatt ttttaaaact cggtaatcca tataatgaag ataaattatc tcttagtgaa
     7741 gaacatgata ctattttagt agttttacca ttagaaaaag gatatgaatg gaattgtctt
     7801 gagatagaag tactatccgc tgctcaatct ctagcaaata tctattgcaa caaagttcgc
     7861 ctcatagatg gtcatgtttg gaaggaataa aagaaaaagc atagttggta atttttagtt
     7921 acgtagggtg ggcttcagcc caccgttttt tcatttttga ttgttgattg gtgggctaaa
     7981 gcccacccta cggttactaa aatcccgtca ttacctcgca ggtgggaatc cggtttgttc
     8041 ggtttcggtt ttttagggac agtgccgcaa cattaaattt ccagattccc actttcgcac
     8101 aaataacagc gcaaataggg caaagataag gatatgggtt tttttctttg aatttactat
     8161 acaattcgcc cgtccccgtt atagaaaccc aagccgatga aaaccctgct ccaactccaa
     8221 accgccgcac aaaacttcgc cgcctactac aaagaccaaa ccgacgaacg ccgcgagaaa
     8281 gacaccttct agaacgaatt tttcgccatt ttcggcatcg accgcaaaaa cgtcgcccac
     8341 ttcgaatacc ccgtcaaaga ccctgccgac aacacccaat tcgtcgatat attttgggag
     8401 ggcatcttcc ttgccgaaca caaatccgcc aacaaaaacc tgaccaaggc caaagagcag
     8461 gcggagcgtt atttgcagga aatcgggcgc accaagccct ctgcgctgcc cgaatattac
     8521 gccgtcagcg attttgccca tttccacctt tatcgccgcg tacctgaaga aggcgtggaa
     8581 aaccaatggc agttcccttt ggaagcattg cccgaataca tcacgcgcgg cgttttcgac
     8641 ttcatgttcg gcatcgaagc caaagtccgc caaattcaag aagaagccga tattcaagcg
     8701 gcggcggcca tcggcaggct gcacgacgcg ctcaaagaag aaggcattta cgaagaacac
     8761 gagctgcgcc tcttcatcac gcgcctgctt ttcctctttt ttgccgacga cagcgccgtt
     8821 ttccagcgca actatctctt tcaagacttt ttagaaagct gcaaagaaac cgacacgctc
     8881 ggcgacaagc tcaaccaact ctttgaattc ctcaacacac ccgaccaaaa gcgcagcaag
     8941 acccaaagcg aaaaatttaa aggtttcgaa tacgtcaacg gcggcctgtt taaagaacgc
     9001 ctgcgcacct tcgacttcac cgccaagcag caccgcgcct taatcgactg cggcaatttc
     9061 gactggcgca a
//
LOCUS       DAXHKX010000070         9052 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHKX010000070 DAXHKX010000000
VERSION     DAXHKX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.070"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(58..1851)
                     /locus_tag="ACFMWZ_001222"
     CDS             complement(58..1851)
                     /locus_tag="ACFMWZ_001222"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC5935187.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2096..3205
                     /gene="proB"
                     /locus_tag="ACFMWZ_001223"
     CDS             2096..3205
                     /gene="proB"
                     /locus_tag="ACFMWZ_001223"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC5935188.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3218..4480
                     /locus_tag="ACFMWZ_001224"
     CDS             3218..4480
                     /locus_tag="ACFMWZ_001224"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC5935189.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRNRIEAGLPPVRFRLDPQAARHIGGEAAGADNFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(4705..7737)
                     /locus_tag="ACFMWZ_001225"
     CDS             complement(4705..7737)
                     /locus_tag="ACFMWZ_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC5935190.1"
                     /translation="MFWIVLIVILLLALVGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKMAVYWLFVGVVRFCRNYLAHESEPDRLVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDAWVVE
                     PPEVPKVPMTAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDNPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNV"
     gene            7902..8582
                     /locus_tag="ACFMWZ_001226"
     CDS             7902..8582
                     /locus_tag="ACFMWZ_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC5935191.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     DSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            8611..8970
                     /locus_tag="ACFMWZ_001227"
     CDS             8611..8970
                     /locus_tag="ACFMWZ_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFC5935192.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQLNRPASALSNIFLHTV
                     GSLLLTWLGLKIGAAVK"
BASE COUNT         1966 a         2559 c         2390 g         2137 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagtt tgctcaaagc
      121 ggaaaggtag gctttggcag tggcaaccaa aacgtcggta tccgcgccct gaccgttgac
      181 gacgcggttg ccgcgtgcca gacggacgct ggtttcgcct tggctttccg taccttgcgt
      241 gacggcattg acggaataaa tctgcaaagt cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatt gcatcaaccg ggccggaacc ggttgcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtactga ttttttggga
      421 aatgaatttg tagctttcgg tattcatatt gcccatttcg tccgacacca gcgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcgtg caaatgccgc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa tcggttagcc gaccaaccga ccgattcggc
      661 agacataatc tcgtaagttt cgcggtgttt cagcaccccg tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat
      781 ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcgccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc attgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttgg caatcagttc gcggaaaaat tcttcggttt tgtacgggat
     1081 tgaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa tcaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 gcgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgactgctc
     1561 gccgtcgcgc agggtggtat caaaaataat tacgcggttg gtctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgcttc
     1681 cgccaacgcg gtcagctttt gcagttgcgc cagaggcagc gagccgcgcg tgcgccattt
     1741 atagatggcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaatctcaat aagccgccat agattttatt aattttatac
     1981 attttgtcca ttttattgca gacgcggcgt gattgttgac aattgcggca gaacagggca
     2041 aattcggcgg cacaaggtgg caacgagaca ggacaaacca acaaggacgg aaacaatgaa
     2101 atacaaaaga atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct
     2161 ctcgcgcggc aaaatccaaa ccatcacctg ccagcttgcc gcattgcatc atgcgggaca
     2221 cgagctggtc ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa
     2281 aaaacgtccg gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct
     2341 gatggaagaa tatacggcaa acctgtcttc agacggcatc gtgtccgcgc aaatcctgct
     2401 cagccgcgcc gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt
     2461 gctgctgcaa cgccgcgccg tccccatcat caatgaaaac gatacggttt cggttgagga
     2521 attgaaaatc ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga
     2581 cctcttggtg ctgctgaccg acatagacgg tctttacacg ggcaacccga acagcaatcc
     2641 cgatgccgta cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg
     2701 cggatcgggt tcggcaaacg gcacgggcgg tatgctgacc aaaatcaaag cggcaaccat
     2761 cgccgccgaa tccggcgtac cggtgtatat ctgttcctcg ctcaaacccg atgcacttgc
     2821 cgaagctgcc gaacatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg
     2881 gacgcagaag caatggctgg cgttctattc cgaaagccgg ggcagcgttt atgtggacga
     2941 aggtgcggaa cacgctttgt ccgaacaggg gaaaagcctg ctgatgtcgg gcattgccgg
     3001 aatcgaaggg catttttccc gtatggacac cgtaaccgtg tacagcaagg caaccaaaca
     3061 gcccctgggc aaagggcgcg tcctgttcgg ttctgccgcc gccgaagacc tgctcaaatc
     3121 gcgtaaggcg aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat
     3181 acgcctgctt ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct
     3241 tgcccttgcc aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg
     3301 cgcgctgctt gctatggcgg acagtctgga agcggcggcg gcagatattt tggcggcaaa
     3361 ccgtcaagac ttggaagccg cggcaggcaa cattcccgaa agcatgaccg accgcctttt
     3421 gttggacggc aaacgcattt gcgcgatggc ggacggcatt agggcggtta ccgcactgcc
     3481 cgaccccgtg ggcgaaatac tggaaacctc gactctgccc aacggcttgg aaatcgtcaa
     3541 aaagcgtgtg gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacgtc
     3601 cgatgcggcg gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga
     3661 tgcattccaa tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg
     3721 catcgacccc gacgcgctcc agttgattga agacaccggg cgcgaaagca gttacgaaat
     3781 gatgagggcg aaagattatc tagacctgct gattccgcgc ggcggggcgg ggctgatacg
     3841 ggcggtggtt gaaaatgccg tcgtgccggt cattgaaacg ggaacgggca tcgtccacat
     3901 ttatatcgac aaggacgcgg attgggacaa ggcgctccgt atcgtgtaca acgccaaaac
     3961 cagccgcccg tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc
     4021 cgacttcctg cccaagctcg aacggctgtt ggttcgcaac cgtatagaag ccggactgcc
     4081 gcccgtccgc ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg
     4141 tgcagacaat ttcgataccg agtttttaga ctacatcctc gccgtgaaaa ccgtcgcttc
     4201 ggtcgaagag gcggtcgggc acatcgaaac ccacggcacg caccattcag acggcatcgt
     4261 taccgaaaac cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta
     4321 tgtcaacgcg tccacgcgct ttaccgacgg tggcgaattc ggcttgggtt gcgaaatggg
     4381 catctccacg caaaaactcc acgcgcgcgg ccctatgggt ttgaaagagc tgacaagtta
     4441 caaatacatc gtacaaggca cgggacaggt tagggaataa tccgaattca aacgtctgat
     4501 atagtggatt aactttaaac cgatacggca ttgcctcgcc ttagctcaaa gagaacgatt
     4561 ctctaaggtg ctgaagcacc aagtaaatcg gtttcgtact atttgtactg tctgcggctt
     4621 cgtcgccttg tcctgatttt tgttaatcca ctataaaaat gccgtctgaa acagtcttca
     4681 gacggcattt ccatttgcaa aaaatcaaac attgtccaag gggacgagaa tcgtacggtt
     4741 gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc atctggtcaa tcagacgcgc
     4801 ggcacggttg tagccgatac gcaaggcgcg ctgtacgccc gaaatactgg ctttgcgcgt
     4861 tttcaggaca acggatacgg cttcgtcgta catcggatcg gtttcgccgt cgccgctgcg
     4921 cccgatgccg ggcagctctt cgctgccgcc gccgctcaaa atatcgtcaa catagtccgg
     4981 ctcgccaaac tgtttcaaat attcgaccac gcggtgcacc tcttcatccg aggcaaatgc
     5041 gccgtgaacg cgctgcggat aggcagtacc cggcggcagg aacagcatat cgccctgacc
     5101 gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg ctgtcgattt tgctggacac
     5161 ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga cccgtgatga catcgacgct
     5221 ggggcgttgt gtggcaagga taagatggat gcctgccgcg cgggcttttt gggcgaggcg
     5281 ggcaatcagt tcttcgattt tcttgcctgc cgtcatcatc aggtcggcaa actcatcaac
     5341 cacgaccacg ataaacggca atttttccaa aggttcggga ttgtcgggcg tgaggctgaa
     5401 cgggttgccg attttctccc cccttgctgc ggcttcggcg attttttgat tgaaacccgc
     5461 aagattacgc acgcccataa agctcatcag gcggtagcgt ttttccattt cgttaacaca
     5521 ccagttcagc gcgtttgccg ccagcttcat atcggtaacg acaggggcga gcaggtgcgg
     5581 gatgccttcg taaatgctca attccagcat tttcggatcg atcataatca tacgcacgtc
     5641 ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg ttgacaccca ccgatttgcc
     5701 cgaaccggtc gtgccggcaa ccaacaaatg cggtgctttg cccaagtcgg ttacgacggg
     5761 ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg gatttggatt cggcaaactc
     5821 gggcgaattg aagatttcgc tcaggcgtat catttggcgt ttcgggttcg gaagttccaa
     5881 acccatacag gttttgccgg ggatggtttc gacaacgcgg atggaagcca cgccgagcga
     5941 acgcgccaag tctttttcca gatttagaac ggaattgccg cgcacgccga catcgggttc
     6001 gatttcataa cgcgtaatca cggggccgga ataagaatcg acaaccttga ccttgacttt
     6061 gaactccgcc aatttttctt cgatggtgat gctgttttcc aacagttctt cttcggtttg
     6121 cgtcgcctcg ggattgaaca gcggaggcag aagcaggtcg gttgtcggca ggtgttcgta
     6181 taccgcatcg gtttcttcag attggaacgc cccttcatcc gcttccgtat ccgatacccg
     6241 gcaggacggg cgttcagacg gcacatcttc aaacggacaa atcgcctgac tgtcatgccc
     6301 gaacgcttcg gtttccgaca gatattgccc gtttgaccgc tctgccgcac cttcactgcc
     6361 gtcatctgca atagcggcgg tttcctcctg ccaacctcca ttcaaaacat catcggcaag
     6421 atggtcggtt tcggcaatgc ggctgcgttg cgcctgctcg aatcctgacg gcggttcata
     6481 ggtacggttg tagatttccg atacgggagg cggcggcgga atatcgattg cggtcatggg
     6541 aactttcggc acttcgggtg gttcgaccac ccaagcatcg ggcgcggcag gttcttcaat
     6601 atggatatcg gcagtttcat cataaaccgg accgccgtat cccacagacg aaacggtttc
     6661 cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc cgtttcccga caaccgtccg
     6721 actttccgga atcaggcgcg aaatccgcgc ctccgcagaa accgttgcct tgttcggttc
     6781 tttcaaattg acggaaacgc ggcggcgggc atcgcggacg gctgtgccgt ctgaaacgga
     6841 ctgcccttgg gaaacatccg gggattttgc ctccgcctgc cctgcatgac ggcggaacgg
     6901 cgcattccgt gcattttcgg ctttgactgc agaaaaggca gacggatgct gcttctcaaa
     6961 atacggctca aatccgtaat ccgcagactc ggaaaacgct tctttgtccg catcgaacat
     7021 acgggaatac gtgcgttgaa gcacagggtc gtccaaaccg ataatctgca gcccttccat
     7081 cggggtatcg gacacgcgga ctttggggac cgctgtttcg tttttctcaa atgcatcgat
     7141 atagcgtttt ttcgtttccc ttaaagccgc gctgtttaaa gcacgcgttg cttcttccaa
     7201 agtgatttcc ttaaaaaccg gacggacggg cgaaatttcg ctttcagacg gcatcaaccc
     7261 ttcagcaata ctccggtcga atgggatgcg gcggttgtcg attacggcag ttgcaatgtc
     7321 ttccgtatcg gcagcctctt cctccgcagc ttctgcttct tccgtttccg cttcttccgt
     7381 cccgtttcca ctgtctgaat acccgtcgga tgcgttcgga acatccgcac ggtttgcaga
     7441 agcaggcgga acgagcctgt ccggttcgga ttcgtgcgct aaatagtttc ggcagaaacg
     7501 gacgacaccg acgaacagcc aatacactgc cattttgacg gcatggaaaa gcatcagggc
     7561 aagttcggga aaatcgggca taccgtcttt gatttcaggc agctccgcct gtttttcccc
     7621 tttcttttcc tgccacgcag aaacctcgcg catccactcg cgttcggatt gtgcgcggac
     7681 aaaaaacaag ccgacaagcg caagcaacaa aataacgatc aaaactatcc aaaacatatg
     7741 ccgtttcccg caagacaaac gattaaacgg gtttatttta acgtgtttcc atcccaaaaa
     7801 aaacaaccgg ccggcaattc tgccgaaacc gattcggctt tcggtttgcc ccagccgaca
     7861 gtataatagc cgtcccttcc cttaatcttt gcggcattgc catgattttc caaacagtct
     7921 ggttttcaga tatggtactg tcggtttcgt ggattgtcct tatcctgata ctggcagctt
     7981 ccgcgccgtc cgcattccgc tcgcttgccc gctaccgttc cgcccttcca ctgtgtaccg
     8041 taattttttc cgcagcatgg tgtctgaacg cctctgccgg cggcggacag cttgcccaga
     8101 tgaactacca cctgctcgcc gtcaatctgg ttgcactgat ggtgggcact tccgccgccc
     8161 tttggcttgc cgcgctgctg atgctgccct actgcctgct gtttgccgat tctgccggcg
     8221 catatccgcc gaacgctttg gtgctgattc tgcccgcgct ggtcgtcaac cgcctgtcgc
     8281 gtatgctggt caaccgcctg ccgcccaata ttttcatctt tattttcgtc aacggctttc
     8341 tcgcttccgc cgccggcatt ctgttgaccg ggctggtgct gaccggtatt ttggatgctg
     8401 caaacgcttt tccgtccgaa atattgtgga cgaccgccct gcccgtcttt attctgctgg
     8461 cgtgggcgga agccttcctc agcggcattt caactgccat atttgttgca ctgaagcccc
     8521 attggattaa cactttcgac gacaaccgct atcttaagtc cgaccgcggc atatggcggt
     8581 agccttcaga cggcatagac aggcaaaacc atgctttcca acatcatccc cctttccatc
     8641 ggtgcggcac tcggtgcaac ggcgcgttgg ctgctcaacc tcgccgttcc tgccgccatg
     8701 tctcccgcca cgggcaacct gtttgccaac tggacgggcg cgctgctcat cggcatcttt
     8761 gccgaaaccg tcaaccaccc acaatggaag ctgctgctga tcaccggttt tctcggcagc
     8821 ctgaccacgc tttccggatt ttctctggaa accgtaaccc tgctccaatt gaaccgtccc
     8881 gcttcagcac tttccaatat tttcctgcat acggtgggtt cgctgctgct gacttggctc
     8941 gggctgaaga taggggcggc agtcaaataa ccgcaacaaa ttgcctccct ttgttttgac
     9001 ataatctata aattagcttc gcttgtttaa atactggctg tgccaattat ag
//
LOCUS       DAXHKX010000071         8940 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHKX010000071 DAXHKX010000000
VERSION     DAXHKX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.071"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            169..1092
                     /locus_tag="ACFMWZ_001228"
     CDS             169..1092
                     /locus_tag="ACFMWZ_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC5935193.1"
                     /translation="MENQRPHLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="ACFMWZ_001229"
     CDS             complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="ACFMWZ_001229"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC5935194.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2693..4672)
                     /gene="tkt"
                     /locus_tag="ACFMWZ_001230"
     CDS             complement(2693..4672)
                     /gene="tkt"
                     /locus_tag="ACFMWZ_001230"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC5935195.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            5199..6695
                     /gene="ccoG"
                     /locus_tag="ACFMWZ_001231"
     CDS             5199..6695
                     /gene="ccoG"
                     /locus_tag="ACFMWZ_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC5935196.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6698..7246
                     /locus_tag="ACFMWZ_001232"
     CDS             6698..7246
                     /locus_tag="ACFMWZ_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC5935197.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(7587..8762)
                     /locus_tag="ACFMWZ_001233"
     CDS             complement(7587..8762)
                     /locus_tag="ACFMWZ_001233"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC5935198.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
BASE COUNT         1934 a         2176 c         2573 g         2257 t
ORIGIN      
        1 aaaattcagg cggtgttttc aaatagcgcg aagcaatagc cgagcttcag ccggtttgtt
       61 tgaaatcaaa gggttggaat taaacgccaa acgcttgaac cattattgcc cgatgcggca
      121 aaccggcatc gtccggcaat acggttgttt aaaaatagga aaatcaggat ggaaaaccaa
      181 aggccgcacc taggcttcgc gttggcactt ttggcggcga tgacgtgggg aacgctgccg
      241 attgccgtgc ggcaggtatt gaagtttgtc gatgcgccga cgctggtgtg ggtgcgtttt
      301 accgtggcgg cggcggtatt gtttgttttg ctggcattgg gcgggcggct gccgaagcgg
      361 cgggattttt cttggtgctc attcaggctg ctgctgctcg gcgtggcggg catttcggca
      421 aactttgtgc tgattgccca agggctgcat tatatttcgc cgaccacgac gcaggttttg
      481 tggcagattt cgccgtttac gatgattgtt gtcggcgtgt tggtgtttaa agaccggatg
      541 actgccgctc agaaaatcgg cttggttttg ctgcttgccg gtttgcttat gttttttaac
      601 gataaattcg gcgagttgtc gggtttgggc gcgtatgcga agggcgtgtt gctgtgtgcg
      661 gcaggcagta tggcatgggt gtgttatgcc gtggcgcaaa agctgctgtc ggcgcaattc
      721 ggaccgcaac agattctgct gttgatttat gcggcaagtg ccgccgtgtt cctgccgttt
      781 gccaaaccgg cacacatcgg aagtttggac ggtacgttgg cgtgggtttg ttttgcgtat
      841 tgctgcttga atacgttaat cggttacggc tcgttcggcg aggcgttgaa acattgggag
      901 gcttccaaag tcagcgcggt aacaaccttg ctccccgtgt ttaccgtaat attttctttg
      961 ctcgggcatt atgtgatgcc tgatactttt gccgcgccgg atatgaacgg tttgggttat
     1021 gccggcgcac tggtcgtggt cgggggtgcg gttacggcgg cggtggggga caggctgttc
     1081 aaacgccgct agttcgcagg caacggaaaa tgccgtccga acgaggtttc agacggcatt
     1141 ttatttgagg gaaagattag cgcggatgaa ccatatcggc aggaacgacc agttcgtcaa
     1201 attcttcgcc cgtcagcaag cccaactcaa cggcggtttc gcgcaacgat ttgttgtttt
     1261 tgtaggcggt tttggcgact ttggcggcgt tttcgtaacc gattttacgg tttaacgcgg
     1321 taacgagcat cagggaatgg tgcaggaaat agtcgatttt ttccggtacg ggttcgatgc
     1381 cgatggcgca gtgttcgttg aagctgttgc acgcgtcgcc caagaggcgg atggattgca
     1441 agaggttgta ggcgataacg ggcatataga cgttcagctc gaaattgccc gacgcgcccg
     1501 ccatgccgat ggtaacgtcg ttgccgaaca cttggcagca caccatggtc atcgcttcgc
     1561 attgggtcgg gttgactttg cccggcatga tggacgaacc cggctcgttt tcggggattt
     1621 tgatttcgcc caaaccgcag cgcgggccgc ttgccagcca gcggatgtcg ttggcgattt
     1681 tattcaggct tgccgccagc gttttcaatg cgcccgaagc ggcaacggcg gcatcgcgtc
     1741 cgcccaaggc ttcaaatttg ttcggcgcgc tgacaaacgg caagccggac aattcggcga
     1801 gtttggcggc ggctttttcg gcgtattcgg gatggctgtt caaacccgtg ccgaccgccg
     1861 taccgcccaa agcaagttca tacaagtctt taagcgcgtc gttcagacgg cctaagccgt
     1921 ggtcgagctg ggaaacgtag ccggaaaatt cttggcctaa agtcagcggc gtcgcgtcct
     1981 gcaagtgggt gcggccgatt ttgacgatag gggcgaaagc ttgggctttt ttgtccaagg
     2041 tatcgcgcag ggcttttacg gcggggatga ggtggcggtt gatttcaatc gcggcggcaa
     2101 cgtggatagc ggtcgggaat gcgtcgttgg tcgattgcgc gtggttcaca tggtcgttgg
     2161 gatggacggg ctgataagcc gccaaacccg taccggcgat ttcgttggcg cggtttgcca
     2221 gcacttcgtt catgttcata ttggactgcg tgccggagcc ggtctgccaa accactaaag
     2281 gaaactgtcc gtcaagcctg ccgttcaaca catcatccgc cgcctgcgta atcaaatccg
     2341 cctgttccgg cttaatcctg ccgagggaaa cattggtcgc ggcggcggct tttttcacca
     2401 gcgccaaagc ataaatcagc ggctgcggca gggtttcgcc gccgattttg aaattgttgc
     2461 ggctgcgctg ggtctgcgcg ccccaatagg cttcggacgg gacttcgaca ttgcccatgg
     2521 tgtcgtgttc ggtacgggtg ctcatgcgtt tctccttttg aaatgtgaat aagagtgatt
     2581 cgcaaatatt ataatggaga ttggaggaat gaggaagtat caaggagaga gggcaaacaa
     2641 atgccgtctg aagccctaat tggtttcaga cggcatttgt ttaggtgtgg ggttacagca
     2701 cggatttcac cgtatcaacc acattgtcca cggtaaagcc gaatgctttg aagagtaaat
     2761 cggcaggggc ggattcgccg aagcggttga tgccgacgac tgcgccgttc agtccgacat
     2821 atttgtacca gccgtcggcg tggccggctt ctacggcgat gcgcggcagg ctttcgggca
     2881 ggacggcggc ttgataggcg gcgtcttggc ggtcgaatac gttggtggac ggcatggaaa
     2941 cgacgcgcac ggcgatgttt tgcgcggcga gggctttttg cgcttccaaa gccagctcga
     3001 cttctgagcc ggtggcaatg atgacggctt gggcgttgcc ttgggcttcg ctgatgatgt
     3061 agccgccgcg tttgatgtcg tccagttgtt gttcgctgcg cgcttggaat ttcaggtttt
     3121 gacggctgaa aatcaggcag gacgggtgat cggcggcttt gacggcttct gcccaagcga
     3181 ccaaggattc ggcggtgtcg cacggccgcc atacgtccat attcggaatc aggcgcaggg
     3241 tggcggtttg ctcgatgggt tggtgggtcg gaccgtcttc gcccaaaccg atggaatcgt
     3301 gggtgaacac aaatacaggg ttgattttca tcagtgccgc catacgcagg gcgttgcgct
     3361 cgtattcgct gaacatcagg aaggtcgcac cgaatggttt gatgccgccg tgcagggcaa
     3421 gaccgttcat aatcgcaccc ataccgaact cgcgcacgcc gtagtggatg tagttgccgc
     3481 ctttgtcgcg ggtaacggag acgctgtttg accagtcggt caggttggac ggggtcaggt
     3541 cggccgaacc gcccaccaat tcaggcaact cttttgccaa gatttcgatg ctgttttggc
     3601 tggctttgcg ggtagcgatg gtttcggctt tggcgcacac ttctttcaat gccgtctgaa
     3661 cgtattcatc gaaattgtcc ggcagctttt tatccatacg gcgcacaaat tctgcggctt
     3721 cggcaggata tttggcttga tattgcgcga acagttcgtt ccagtcggct tccagtttcg
     3781 cgccttgttc tttggcattc cacgcatcgt aaatttcttg cgggatttca aaggcggggt
     3841 aagtccagcc caaatgtttg cgcgtggctt cgatttcgtc cgcgcccaaa ggtgcgccgt
     3901 gggttttgtg gctgccttct ttgttggcac tgcctttgcc gattaaggtt ttgcagcaga
     3961 tgatggacgg tttgccggtt tcggcacgtg cggcttcgat ggcggcttga atggcggcgg
     4021 tgtcatgacc gtttacgtta ggaacgacgt gccagccgta gctttcaaag cgttgcggga
     4081 tgttttcggt aaaccagccg tccactttac catcaatgga aatattgtta tcgtcataca
     4141 aaacaatcag tttgcccaag cctaaagtgc cggcgagcga acaggcttcg tgcgatacgc
     4201 cttccatcag acagccgtcg cccatgaaga cgtaggtgta atgatcgacg atgttcaaac
     4261 cgtctttatt aaattcggcg gcaaggattt tttctgccaa tgccataccc accgcgttgg
     4321 caatcccttg ccccaacggg ccggtcgtgg tttccacgcc gtcggtgtag ccgtattcgg
     4381 ggtggccggg ggttttgctg tgcagctggc ggaagttttt caagtcttca atgctcagat
     4441 tgtagccggt caggtgcagc aggctgtaca acagcataga cgcgtggccg ttggagagga
     4501 cgaagcggtc gcggttgtag aatttggggt tggcggggtt gtgattgagg aatttcgtcc
     4561 acaatgtttc cgccatttcc gccataccca taggcgcgcc ggggtggccg gaattggctt
     4621 tttgaacggc atcggccgag aggaagcgga ttgcgtttgc cagttgagac attttgtatt
     4681 ttccttgctg gtgtttcaga taagtggata atcggaaagc gttgattatc gcccgattcg
     4741 cttatgcttt caagaaaagg gcggacgcgt gggaaggcgg cggcagggga caggcgggga
     4801 aggattttcg atttgcgggc gaagcctgcc attattcctt ttgaaataaa aagttataga
     4861 ttgtgtgccg gattgtcgat agcgtttgtt tatgagcttg cgccgtcggt tctgccgata
     4921 tgggggtgtc ggttttttta gtcttttttt taaccgtatt cggattttgt tcgggcggta
     4981 aggtaaaatc caggcgtttt gatgcggatg ggaaatgtat ccgccccata catccggacg
     5041 gcgcataaag ttgtacaata gcggaaatat attttgggaa aaacggattt tctcaaaact
     5101 tgaaacacaa cgccttaaaa aacaagaaaa atgacgtctg aaaagcaaac ggcggcggta
     5161 acgggcaaac catcaatcaa aaatctaagg aatgcagaat gaccacggaa aaccaagccg
     5221 gcagtccggc atccggaatc ggcacgtccg aacaaacaaa agccgcgccg aaagcaaaaa
     5281 aaacattcga tccgcgagcc agcgtgattc aaatccatcc cgaaggcgaa cgcatccatc
     5341 ccaaaaaggc ggaaggacgg ttcgccaaac tgcgtatcgc cgccgtattg gcgacgcagt
     5401 ttgtgtttta cgtcattccg tggttcaact ggagcggcag gcaggccgtc gttttcaata
     5461 tccccgaacg gcatttcttc attttcggat tgtcgttggg ggtgggcgat ttgatttacc
     5521 ttgccttgct gctgatgatt tgcgccttcg ggctgttttg gtggacgacg attgcagggc
     5581 gactgtggtg cggctattcc tgcccgcaaa cggtttacac cgaaattatg ctgtggattg
     5641 acaacctggt cgaaggcgat agaaacaaac ggctgaaact ggaaaaatcg ccgtggaatt
     5701 tcactaaaat ccgcatcaaa gccaccaaat acctgctgat tttccttgtc tgcgcgtgga
     5761 cgggcatcac gtttgcaggc tggtttgtcc ctatccgcca gttcgttccc gatttattca
     5821 ctggagcagc aggtggcggc gcgatgtttg ccgcagcgtt ttatggcttt atgaccttct
     5881 tcttcgccca cattatgcgt gaaaaagtat gcctgcatat gtgtccgtat gcacgtttcc
     5941 aaagcgcgat gttcgacaag gacacgctga ttgtttctta tgacgcggaa cgcggcgaac
     6001 cgcgcggcgc gcgcaagaaa acggtcaata aggaagaggc gggtttgggc gactgcatca
     6061 actgtgcgat gtgcgtccaa gtctgccccg tcggcatcga catccgcaac ggtctgcaat
     6121 accaatgtat cggctgcgcc gcctgtatcg acgcgtgcga tgagattatg gacaaaatgg
     6181 gctatccgcg cggattaatc cgttatacga ccgaaagcgc gctggaacac gaatattctg
     6241 aaaaagacat taaaaaacgg ctgctcagac cgcgcgtggc aggttacggc gcggtgttgg
     6301 cgttagttat cattgccttc ctggccggtt tgtccacgcg caaaatggtc gaggtcgata
     6361 ttttgaaaga ccgtggcgta ctggtgcgcg aaaacgccaa aggctggctg gaaaacgcat
     6421 acagcctgcg tatcatcaac aaaagtgaaa aagaacagct cattaccgca agtgtcaaag
     6481 gctttgacga aatcgccctg accgggctgc ccgaaggcgg tatcaaggtt gccccgcgcg
     6541 aaacggtaac ccttcccgtc caagtgtcca ccattccgga atacgcggac aaaggcagcc
     6601 accctatcga atttaccttc caataccgcg aaagcggcgc gcccgacggc aagccggtcg
     6661 tcttggaaga agatgcaacc tttatcggag aataaccgtg tctcaaaaca ctccaatcaa
     6721 accttggtac aaacacgtct ggccgtggat cttgatggcg gggccgattt ttgtcgtcat
     6781 cgccagcgtc gctatgtttt ttgtcgcgca gcagcacgcg acagatttgg ttacggacga
     6841 ttattataaa gacggcaaac atatcgacat ccagcttcat cgggatgaag aagccgtcag
     6901 acggcatatc ggggtgcagg ttctcatttc ccccgatatg aatgcggcaa aagtgtttgt
     6961 cggcggcgag tttgacggca aacagccttt gaacctgctg ctgatgcacc cgacccgcaa
     7021 ggcggacgat caaaccgtcg ccctcaagcc cgtcggcagc gcgcagaacg gcagggcgga
     7081 atatgaggcg gtgttcaaaa ccctttcgcc gaccaaccac tggtatgtgc gcgtggagga
     7141 cgcggcaggc gtgtggcgcg tcgagaacaa atggattacc agccaaggca atgcggtgga
     7201 tctgaccccg atggataaac ttttcaataa tgccggaagc aaataaaaaa gccgtgtttt
     7261 cattgcattg gtttgccaga tgcaatgtcg ggcaggcatt gtttgattat tcggcgtgat
     7321 ggtctgattt atcggacgaa aataaatgta tcttatcccg tttatttttt aacaccattc
     7381 cgaaatgcag cctgaaaaat gccgtctgaa gggcttcaga cggcatttgt gttgtatgcc
     7441 cgacacaatg cggaaggcgt tttgtttaca taaaaacgtt tttatttctc tccgtttttt
     7501 cgacactatt ccgaaacgca gcctgaaaaa tgccgtctga aaggcttcag acggcatttt
     7561 gtttgcaaat caaatcctac ctgatgtcag cgtccgagcg ttgcaacagg ttcgctcccc
     7621 gtcagcagat attgtatcgt ctcgcggacg gtgcggatgg cattgccgaa gggcagcggg
     7681 tctttacctc tgaagaacag gcctttatct acttctccac ggaatgcggc ggcaagctgg
     7741 atatcaatgc agaattgtcc ggctttggaa agcccgtcgc gcagaccgca actggtcagg
     7801 cagtttaaac cttgggtaca gcggcgcggg tcagctttgg cgtttgcctg aagtttgctt
     7861 tcacgcttga tgtagctgtc taggaatttg gtgcggacac cgcgcgccgg caaaccggca
     7921 acagacataa attcaactac tttttcagtt tccgcaccgg cgagcgtttt tttgaagtta
     7981 aggtgtgcat ctccttcttc ggtaacggca aaagccgtac cgatttgaac ggcggatgct
     8041 ccccagttct ttagggcggt tttgactttt tcaaaatttg ccatgcctcc cgcaagaata
     8101 agcgggattt tttcgctttc cagccctaaa cttttgaaaa cttcaaacgt ttcctcaatc
     8161 acgcgtttga agtcgaactt ggcatcgttt acgccttcaa cggttgatgc acccaaatgt
     8221 ccggccgcgt gggcaggatg ttcgactaca atcgcatcgg gcaatatgcc ttttttcatc
     8281 caacgtttca agacgatatt aataccgcgc gattcggaca gaatcggcag cagcgcgaca
     8341 tctttatgat agccctcggt catttccggc aggtctaaag gcaggccggc acccattaca
     8401 accgcatccg cccctgattc gcaagcctgg cggacatacg cggcgtggtc tttgaccgcc
     8461 ttcatcacgt tgaccgcaat cagtcctttt ccctctgaag cgcttttggc tttttggatt
     8521 tccctgtcta atgcggtaca gttcaaagat gtatatttct cttcactcgg attgatttgt
     8581 gattcggcga gtaggtcttc gtgaaggtgg cgcaaatcca cactggcaat cgttccgata
     8641 ccgttttcac gtgccaccgc gctggataaa cccgatgcgg aaacaccgac ccccataccg
     8701 ccttgcacga tgggggtaag ggattttcca cgaataacca aagggtcaaa aatattttgc
     8761 atcagtttct ccgagtatac gaatcagtca ggctcatgaa aaatggttct aactgtttta
     8821 gaactattgc tcaaatttgg tattatactc taaatatcgc ggcggtaaac aagaaagcag
     8881 ccggagcttc gggcatattc tgttacgcct gtttcaatcg ggagagaaaa tattatagtg
//
LOCUS       DAXHKX010000072         8918 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHKX010000072 DAXHKX010000000
VERSION     DAXHKX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.072"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            37..669
                     /gene="pdxH"
                     /locus_tag="ACFMWZ_001234"
     CDS             37..669
                     /gene="pdxH"
                     /locus_tag="ACFMWZ_001234"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC5935199.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(798..1808)
                     /locus_tag="ACFMWZ_001235"
     CDS             complement(798..1808)
                     /locus_tag="ACFMWZ_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC5935200.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQIGGHSEQALSEAEKAQGKILMCCTTAQSDISLNIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(1900..3330)
                     /gene="gatB"
                     /locus_tag="ACFMWZ_001236"
     CDS             complement(1900..3330)
                     /gene="gatB"
                     /locus_tag="ACFMWZ_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC5935201.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEVEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKAGLELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            complement(3373..4305)
                     /locus_tag="ACFMWZ_001237"
     CDS             complement(3373..4305)
                     /locus_tag="ACFMWZ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC5935202.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            complement(4302..5747)
                     /gene="gatA"
                     /locus_tag="ACFMWZ_001238"
     CDS             complement(4302..5747)
                     /gene="gatA"
                     /locus_tag="ACFMWZ_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC5935203.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            complement(5810..6100)
                     /gene="gatC"
                     /locus_tag="ACFMWZ_001239"
     CDS             complement(5810..6100)
                     /gene="gatC"
                     /locus_tag="ACFMWZ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC5935204.1"
                     /translation="MALTLADVDKIARLSRLHLTAAEKEKSLQELNDIFAMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            6251..6904
                     /locus_tag="ACFMWZ_001240"
     CDS             6251..6904
                     /locus_tag="ACFMWZ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC5935205.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            6909..8252
                     /locus_tag="ACFMWZ_001241"
     CDS             6909..8252
                     /locus_tag="ACFMWZ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC5935206.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGAWLDEAGTLKAIEHTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLVRAAAEACYSRFRDAGQSCNAAKRIIVTEAVADRFIPLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWHEEVFGPAALILRAANEDHAIALANDSPFGLGACIYTADTDRA
                     WQFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(8301..8735)
                     /locus_tag="ACFMWZ_001242"
     CDS             complement(8301..8735)
                     /locus_tag="ACFMWZ_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC5935207.1"
                     /translation="MNFALSVITFTLASFLPVPPAKAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNDVNKYCR"
BASE COUNT         1718 a         2164 c         2743 g         2293 t
ORIGIN      
        1 tggtttttgt taatccacta ttaagaggag cggtaaatgg atttgcacaa tattcgggaa
       61 gattacagca aacgggaatt gtcggaaggc gattgcgccg acaatccgat cgagcagttc
      121 gagcggtggt tggacgaggc ggtacgcgca caggtcaacg agccgacggc ggtcaatgtg
      181 gcggcggtcg acggacgcgg tagacccaac agccgtatgg tgctgctgaa ggaagttaat
      241 tctgaaggtt ttgttttctt tacgaattat cacagccgca aggggcgttc gctggatgct
      301 catccatttg cggcgatgac gtttttttgg ccggagctgg agcgtcaggt gcgcgtggaa
      361 gggcgtgttg aaaggctggc ggagaagttg tcggacgaat attttgagag ccgcccgtat
      421 cagagccgtt tgggtgcgtg ggcaagtgcg cagagcgagg tgattccgaa caaggcggta
      481 ttggtggcaa aggcggcggc ggtcggactc aaacatcctt tgcacgtgcc gcgtccgccg
      541 cattggggcg gctatattgt gattcccgat ttgattgagt tttggcaggg caggccgagc
      601 aggctgcacg accgcatcca ataccgtttg ctggatggtg gctggatacg cgagcggctg
      661 tcgccttgaa caggaaaaaa catatgccgt ctgaaaaccg gaatcgggct tcagacggca
      721 ttttttatgg ggaaaatacg gacgggaagt tttttgtgtt ccgaacggaa agcctgaatc
      781 ttttttatac cggggaatta tgatgcggac ggcgtgaatg cgtcggagaa aaacaagttt
      841 tccggcagct tatgctgttg cacaaacaga ttttttgctt gttcggtcat ggccggagag
      901 ccgcaggcaa atacttcgta ttccgacagg tcggggtggt cttgtgccgc gatgtcttgt
      961 acgtgccctt ttcttccctg ccagccctct ccggggcggg acaatacggg ggtgaagcag
     1021 gcgtttttca gacggcatgc caacccttgt gcttcttcga gggcatacaa atcatcttga
     1081 tgacgcgcgc cccagtagaa atggacggcg cggccgctgt cttggcggat aaggtcgagc
     1141 aggatgctgc ggatgggggc gtagcctgtg ccggttgcca gcaggatgac gggtttgccg
     1201 ctgtcttcct gcagggtaaa cgaaccgagc gggcctttaa cgcggacgat gcctttttct
     1261 ttgactttgg gttcgctgcc gaaaatcatt tccgagcaga caccgttttc gcgcctgcgg
     1321 atgtgtagtt ccaaaatgcc ttcttggtcg ggcgaattgg cgatggagta gctgcggctg
     1381 acgttgcccg gcagcagtaa atcgatgtat tgcccagcat agaaggcaaa cggcggggct
     1441 ttgggcaggg caagtttcaa gagggcgaca tcgtgtttga aaaccatact ttcgatgcgt
     1501 gcgggcaggg tgcggacggg tagggcatcg gctttgtagc cggggatgtt gaggctgatg
     1561 tcgctttgcg cagtggtgca gcacatcaaa atcttgcctt gcgctttttc tgcttcggat
     1621 aaagcctgtt ccgagtgtcc gcctatttga atatcgccgc tgaccagttc ggctttgcat
     1681 tgcccgcaga caccgctttt gcaggaatgg ggcagattga ggttttgacg ggcggcagcg
     1741 gccaaaacgg tttcaccgtc gccggcggca aaggtggttt ggtcgggcag ggtaacggtg
     1801 tggttcatgg aatattgttt tttatgctgt tttaatgtgg tcagactgtg aaaacgccgt
     1861 ctgaacgtcg gatctgggtt tcagacggca tcttgcttat caggcaagtt tggctttaat
     1921 cagttcttga acttgcgcgg ggttggcttt gcctttgctg gctttcatca cttgtcccac
     1981 aatcgcattc agggcttttt cgttgccgga tttaaactgt tccacggctt tggcgttgtt
     2041 tgctagcact tcgtccacca tcgcttcaac cgcgccggta tcggtcatct gttgcaaacc
     2101 gtgtttttcg atgatttcgg caatggtggc ttcgggttct gcccacatgg cttcaaaggc
     2161 ttttttcgcc agtttgccgc ttaatgtgcc gtcggcgatt ttgcctacca gcgcggcgag
     2221 gcgcggggcg gtaatcgggc tgtcggcaag ttccaggccg gctttgttca gcgtggcggc
     2281 gagttcgccg ttcatccagt tggcagtcgg cttgccttgt ccgctctctt tggcggcttc
     2341 ttcaaaatag gcggcctgtg cgcggcttgc ggtgagcagg cgcgcgtcgt attcggacac
     2401 gccgtaatcc gccacgaaac gcgctgccat ttctttcggc agctcgggca tttctgcttt
     2461 ggctttttgc atttgggcgt ctgaaatgat gacgggcagc aaatcagggt cggggaagta
     2521 gcggtagtcg tgcgcgtctt ctttcaagcg catcacgcgg gtttcacctt tttcagggtc
     2581 gaacagcatg gttgcctgct gtactttgcc gccgtcttcc aaaatctcga tttgcgcttc
     2641 cacttcgtaa ttaattgcct gctccaaaaa gcggaaggaa ttgaggtttt taatctcgcg
     2701 gcgcgtgccg aactctgctt gacctttcgg gcgaacggat acgttggcat cgacgcggaa
     2761 cgaaccttcc gccatattgc cgtcgcaaat gttcagccag gttaccaagc tgtgcaaggc
     2821 tttggcgtag gcaacggctt cggcggcgga gcgcatttcg ggttcggaca ccacttccaa
     2881 taaaggcgtg ccggcgcggt tcaggtcgat accggtcgcg ccgttcaagc cttcatgtac
     2941 ggacttgccc gcgtcttctt ccatatgggc gcgggttacg ttgatggttt tcacatcgtc
     3001 gccgactacg atttccaatt tgccgtgttc gacaatcggt aagtccaact ggctgatttg
     3061 atagcctttt ggtaagtcgg gatagaagta gtttttacgg tcgaacacgt ttttccggtt
     3121 gattttcgca tccaaagcca agcctaattt gatggctttt tcaacgactt cacggttcat
     3181 taccggcagt acgcccggca aagcacattc caccacgccg gcgtgcgcgt tgggttctgc
     3241 gccgaatgcg gtcgatgcgc cgctgaagat tttggatttg gtgttcaatt ggacgtggat
     3301 ttccaagccg attacggttt cccaggtcat aagagcttct ttctctaaaa tatcaattcg
     3361 atagtcttcg cgtcaggctg tccggcggag cggtttttcc gtttggatgc cgtctgaaat
     3421 gccgcgtgct tctgttttca gaccgtcctt catctctgca accatgttgt ccagcatttc
     3481 ataacgcttg cggtacatcg agcgtttttt gctttcgatg tcggcaaggt ctttgcgggc
     3541 gggggtttgg gggagatgcc agtagccgtc ccgttccaaa cttgcgccgt attcctgcca
     3601 gaatgcgtcg taattcattt tgacgcgctt tttaagtttc cagcgcagtt tgacctgatg
     3661 tttttgtgca atgcccattg cgccgtccaa gccgagtacg gcggcgaaat attgcagggc
     3721 ggttaccatc agttgttggg gacgcaagcc gtggagttgt ttggttatgc ggcggacggt
     3781 gtctttggct tcttcacccg ccggcccttg tacggaggct gtcagcaggt gtgtgccgac
     3841 aaaggcgaag gtcgccatat acagccggtt gcctgcttcg tcgcgcaaag ataaagacca
     3901 cgcgccttct tcgacgcagt tgtcgttgcg gttgagccac aagctgatgc cgtctgaaag
     3961 gtgcgccaag accagatgga agcggtagtc ttccatttgg tgcaggatgt ctgtgccgaa
     4021 cagtttttcc gccatgagaa aatctgccgt catcgcatct aaacggcgtt tgcagttaaa
     4081 acgcctgtca acaaattcgc gcagcagcgg ataggcattt tgcgggcagt ggaggaacag
     4141 cccctgacgg ataggggatg cgttgacaaa ctgctcgaag gcgtaaattt ctttttttcc
     4201 gaaaaatttt cgcaatgtgt attttaaatg ttgcagccga tataaaggcg catagctgta
     4261 aacggtgcga aaatcgggga aggtaaaggt gcggttctgc ttcattcggg tcgtttgccg
     4321 tgccaatcgc tgttgagttg gatttgatgc gccgcaccga ggattttggc ttcggcgaag
     4381 tagttgccga caagctgaac gccgacgggc agtccgccgc cgctgaagcc tgcgggcagg
     4441 gtcaatgcgg gcagtccggc gaggttgacg gcgatggtgt agatatcgct caagtaggtt
     4501 tcaaccggcg aagcatccgc tccgattttt ggggctgcag tgggtgcggt cggcgcgagg
     4561 atgaggtcgc accgtgcaaa tgccgtctga aaatcatcgg caacgaggcg gcgcagtttt
     4621 tgggctttga gatagtaggc atcgtagtag ccgtgcgaca gtacataagt gccgatcatg
     4681 atgcggcgtt tgacttcgct gccgaaacct tcggcgcggg ttttgccgta catttcttcc
     4741 aaatcggcga attgggcggc acggtgtccg taacgtacgc cgtcgtaacg tgaaaggttg
     4801 gtgctggctt ctgcggaggc gaggacgtag taggcgggga tggacagctt ggtttgcggc
     4861 agggaaactt caatcaattc cgcgccttgg gctttcagca aatcaatggt gttttgcaat
     4921 gccgtcagaa catcggcgct gttgccttcg ccgaaatatt ctttgggcag gccgattttc
     4981 aaacctttga gcggttggtt caaatcgcgg gtgtagtctt ctttttcgcg ctcgaggctg
     5041 gtggagtctt tggggtcgaa acctgccatc gcgtttaaca gaatcgcgca gtcttcggca
     5101 gtttgcgcca tcgggccggt ttgatcgaag ctggaggcgt aggcgaccat accgaagcgg
     5161 gaaaccgtgc cgtatgtggg tttgatgccg gtaatgccgc agtgcgatgc gggttggcgg
     5221 atagagccgc cggtgtccga accgagcgcg gcaggggcga ggcgcgcggc aacgacggcg
     5281 gcggaaccgc ctgacgaacc gccggggacg tgctcaagat tccatgggtt tttggctgca
     5341 ccgtagaatg agttttcatt ggtcgaaccc atagcgaact catccatatt ggtgcggccg
     5401 agcgttacca taccttcgtc gagcaggttt tggacgacgg tggcggtgta gggggagatg
     5461 aagttgtcga gcattttgga agcgcacgcg ctgcgccagc cggtttggca gaaaatatcc
     5521 ttgtaggcga cgggtacgcc ggtaagcgcg gaggcgttgc cctgcgcgat acgttcgtcg
     5581 gcggcacggg cttctgcaag ggttttatct tggtcgatgg tgatatagcc gttgagggcg
     5641 ggattttttt cggcgatggc ggcaaggtat gcgcttgcca gttcgacggc ggaaatctgt
     5701 ttggactgca acaggacgct tgcctgtttc aatgtgtatt gggtcataaa tcttccatcc
     5761 ttaaagggtt tcaggcggta ttgctgatgc cgtctgaagc atattcggat tattcttcga
     5821 taacttgcgg tacgatgtac agacggttgc gtacttctgg agcaaccgcc tgatattcgg
     5881 cggcgcggtc ggtttcggtt acttcgtctt cgcgcaggcg cagggcggcc tcgtgcggat
     5941 gcgccatcgg ttcgatgccg tctgtgttga tgttctgcat ctgttcgacc atagcgaaaa
     6001 tgtcgtttaa ttcttgaagc gatttttctt tttctgccgc agtcaggtgc agtcgggaga
     6061 gtcgggcgat tttgtctacg tcggcaagtg tcagtgccat gaaaagcatc cttgtctgat
     6121 ggtaaagata ccgtcgttcg ggtatgatgg ggcgtattgc ttgaattttt aattataacg
     6181 gaaaaatccg ttgcggcggc aatttgcagc atcgaatgcc tgccgcacgg gtttgaagga
     6241 gggggaaata atgggattgg gtatggaaat cggcaagctg attgtggctt ttttggtgct
     6301 gatcaatccg tttagcgcgt tgtcgcttta ccttgacctg accaacgggc acagcacgaa
     6361 ggagcgcagg aaggttgcgc ggacggccgc cgttgccgtg tttgccgtga ttgcggtatt
     6421 tgcgctgatc ggcggtacac tgttgaaggt tttgggcatc agcgtcggtt cgtttcaggt
     6481 cggcggcgga attttggtgt tgctgattgc catttctatg atgaacggca acgacaatcc
     6541 cgccaagcag aatctcggcg cgcagccgga aacggggcag gcgcgccccg cccgcaatgc
     6601 cggagcgatt gccgtcgtgc ccatcgccat accgatcacc atcggcccgg gcggtatttc
     6661 gaccgtgatt atttacgctt cggcggctaa aacatacggc gacatcgcgt tgattatcgc
     6721 ggccggtttg gtggtcagtg cgatttgtta tgccatttta atcgttgccg ggaaggtcag
     6781 ccgcctgctg ggcgcgacgg ggctgacgat tttaaaccgt atcatgggta tgatgctggc
     6841 ggcggtatcg gtggagatta ttgtgtcggg actgaaaacg atattcccgc aactggcagg
     6901 ttgattcgat gtttcacagt gtcaatgtat ttacgggcga aacgctttac cgccgtccag
     6961 ctcaagatta tgcggagttt gaacggcggc tggcggattt gaaaatgcgc ggcggggcgt
     7021 tcgcgcaact gggcgtaacc gaacgtgccg cgcgtctgca aaaatttgcc gaccgtttgg
     7081 aggcggagaa agagcgtttt gcggaaatgg tgtgcgaaga agtcggacgc tgcctgcacg
     7141 aatgccgtgc ggaaatcggc aagtctatcg aactgatacg ctattacgcc cgcctcgctc
     7201 ccgaactgct cgcccacaaa accatcgcga cgcaggcgag cctgagtcag gtgcgcttcg
     7261 agcctttggg tgtggtgttt gccgtcatgc cttggaacta ccccgtctgg caggtattgc
     7321 gtttcgccgt tcccgctatg tgcgcgggca acgcgtgcgc cgtcaaaccc gcgcccagcg
     7381 tggcacgcgt cagtcaggcg ttgtttgatt tggcttcaga cggcattcct ttggcaggcg
     7441 cgtggttgga tgaggcgggc acgctcaaag ccatcgaaca caccgatgcg atggcgttta
     7501 ccggttcgac gcacacaggg cgcatccttg cggcacacgc gggcgcaaac ctcaaaaaaa
     7561 ccgtgttgga gctcggcggc agcaacgcct ttatcgtgat gcccgatgcc gatttggtgc
     7621 gtgcggctgc agaagcctgt tattcccgtt tccgcgatgc ggggcaatcg tgtaacgccg
     7681 ccaagcgcat catcgtaact gaagcagtag ccgaccgctt tattccgctg tttcttgccg
     7741 aatgtgccaa attgaaaatg ggcgacccca aacatcccga taccacgctt gcaccgctgc
     7801 accgtgaaga tttgcgggac cgggttcacg ggcaggtcga agatgccgtt tcaaacggcg
     7861 cggtatgcct gaccggcgga aaaatcccac aagggcgagg ctggttctac cccgcgactg
     7921 ttttagacag ggtaaatcct gcctgccggg tttggcatga agaagtattc gggccggccg
     7981 ccctgatttt gcgtgctgca aacgaagatc atgccatcgc ccttgccaat gattccccgt
     8041 tcgggctagg tgcctgtatt tataccgccg atactgaccg ggcatggcag tttgccgaga
     8101 aaatacaggc gggatcggtg tttatcaacc gccataccag cagtgatttg cgcctgcctt
     8161 tcggcggagt caaagattcc ggttacgggc gcgaattgtc cgaattcggg ctgtacgagt
     8221 tcgtcaacgt taaaacctac tggcagaaat aaacaaaccc cgtgccgttt cagacggcat
     8281 ggggttttcg atgaggctgc ttaacggcag tatttgttca catcgttatt gtatttccga
     8341 atcaaatcat ctttgttttt cgcatttgag tttcccaccg ccttcaggtt catttttgaa
     8401 atccggcagt tttcttcttt gttctgccgt tcggtttcgg caattctttt cttttcttcc
     8461 tcaagctgcc ggtttttctc ggcagtgtcg ggattgtttt cctgtgccgc gccgtctgtg
     8521 cgcttccctg cgtcggcttg ggcgggtttg accgccggtt tggtttggcg cgtccgcagg
     8581 tttaagattt ggctttggtc gggatgaagc tgtttcggta catccgaata gctgttgccg
     8641 ccgccgtcct tccaagtaaa gacggcggct ttggcaggcg ggacaggcag gaaagaggcg
     8701 agggtaaatg tgatgacgga taaagcaaag ttcattaggg tttccttgat ggctgactgc
     8761 cggggcagaa tcgggattgg ctcgggcggt gtcttttccg ggttcgggtc aaagtgggga
     8821 agacactccg ccttaatatg gtgctttata tattggggtg aaacagattc ccaaaaatat
     8881 agtggattaa caaaaatcag gacaaggcga cgaagccg
//
LOCUS       DAXHKX010000073         8745 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHKX010000073 DAXHKX010000000
VERSION     DAXHKX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.073"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="ACFMWZ_001243"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="ACFMWZ_001243"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC5935208.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPESLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDEKLAFAKESGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1458..1847)
                     /locus_tag="ACFMWZ_001244"
     CDS             complement(1458..1847)
                     /locus_tag="ACFMWZ_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC5935209.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVK
                     STLLMNAQNLERYYRQKGTFDNYDQTKLKQNKYFKITLSKVNPDHFTLQAVPDPTTNE
                     GETCIVTLNDGGTLSAAGNGQSCPDFD"
     gene            2073..3251
                     /locus_tag="ACFMWZ_001245"
     CDS             2073..3251
                     /locus_tag="ACFMWZ_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC5935210.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATAKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3317..5251
                     /locus_tag="ACFMWZ_001246"
     CDS             3317..5251
                     /locus_tag="ACFMWZ_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC5935211.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDDIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5570..6352)
                     /locus_tag="ACFMWZ_001247"
     CDS             complement(5570..6352)
                     /locus_tag="ACFMWZ_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC5935212.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6491..8680
                     /locus_tag="ACFMWZ_001248"
     CDS             6491..8680
                     /locus_tag="ACFMWZ_001248"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC5935213.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
BASE COUNT         1986 a         2267 c         2534 g         1958 t
ORIGIN      
        1 gacgtgttgc gataagggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatgg ccgcagccgc attctttttt catatcgatc accatacgac
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgtgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagcccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgccggtttt ttcctgaatc actttggcag cgtcttcttt ggcggcgttg acaaccaaat
      541 ctgcgccgct ttctttggca aaagccagtt tttcatcgtt gatgtcgatg gcgacgacgt
      601 gtgcgccaaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cctgcgccgt
      661 aaatcgcaat ccattggccc ggacgaacgc cggaaacttt aatggcttta taagtggtta
      721 caccggcaca agtaatgctc gaagcttgcg caggatccaa gctttctggg actttgaccg
      781 cgtaatcggc actcacgata cagtgggtcg ccatgccgcc gtcggcagta tagccagcgt
      841 tcaatacaga gcggcacagg gtttcgcggc cggtattgca gtattcgcac gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccttcgt gtcccaacac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 atgcctcgcc gtattccaac gggcgaacct cgcgttcgat tacttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca gcttggaaag
     1321 gcgcggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 tgggcaaacc cgtaaagata ccgtctgaag ctgtgtccgg acggcatctt tacgggtttg
     1441 cgggcttcgg cggaggatta gtcgaagtcc gggcaggact gcccgttgcc ggcggcggac
     1501 agtgtgccgc cgtcgttgag cgtgacgatg caggtttcgc cttcgttggt cgtcggatcg
     1561 gggacggcct gaagggtgaa gtggtcgggg ttgactttgc ttaaggtaat tttgaaatac
     1621 ttgttttgtt tcagtttggt ttggtcgtag ttgtcaaacg tccctttttg gcggtagtaa
     1681 cgctctaagt tttgcgcgtt catcagcagg gtggatttga cttccgacag gcggacgcgc
     1741 cggatgtagg ttttatagga agggtaggtg atgagcgtca ggatgccgag gatggcgacg
     1801 gcaatcatca gctcgagcag cgtaaagcct ttttgaacgt ttttcatagc aatgtgtttc
     1861 catttgtttg tcggtcgacg gcattataac gccgtatcgg aaatggcgga atatgtaaac
     1921 ggattgaaat tttcgggaaa gcagattgta taagccattt aaaacaaatg gttattttta
     1981 ttgtcggcag tttgccgcct tggatggggc agggacttgc ggtagaatcc gcttccgatt
     2041 tatgggattg acgcatacag agaattgaaa acatggcaaa aatgatgaaa tgggcggctg
     2101 ttgcggcggt cgcggcggca gcggtttggg gcggatggtc ttatctgaag cccgagccgc
     2161 aggctgctta tattacggaa acggtcaggc gcggcgacat cagccggacg gtttctgcaa
     2221 caggggagat ttcgccgtcc aacctggtat cggtcggcgc gcaggcatcg gggcagatta
     2281 agaaacttta tgtcaaactc gggcaacagg ttaaaaaggg cgatttgatt gcggaaatca
     2341 attcgacctc gcagaccaat acgctcaata cggaaaaatc caagttggaa acgtatcagg
     2401 cgaagctggt gtcggcacag attgcattgg gcagcgcgga gaagaaatat aagcgtcagg
     2461 cggcgttgtg gaaggatgat gcgaccgcta aagaagattt ggaaagcgca caggatgcgc
     2521 ttgccgccgc caaagccaat gttgccgagc tgaaggctct aatcagacag agcaaaattt
     2581 ccatcaatac cgccgagtcg gaattgggct acacgcgcat taccgcaacg atggacggca
     2641 cggtggtggc gattctcgtg gaagaggggc agactgtgaa cgcggcgcag tctacgccga
     2701 cgattgtcca attggcgaat ctggatatga tgttgaacaa aatgcagatt gccgagggcg
     2761 atattaccaa ggtgaaggcg gggcaggata tttcgtttac gattttgtcc gaaccggata
     2821 cgccgattaa ggcgaagctc gacagcgtcg accccgggct gaccacgatg tcgtcgggcg
     2881 gttacaacag cagtacggat acggcttcca atgcggtcta ctattatgcc cgttcgtttg
     2941 tgccgaatcc ggacggcaaa ctcgccacgg ggatgacgac gcagaatacg gttgaaatcg
     3001 acggcgtgaa aaatgtgctg attattccgt cgctgaccgt gaaaaatcgc ggcggcaagg
     3061 cgtttgtgcg cgtgttgggt gcggacggca aggcggcgga acgcgaaatc cggaccggta
     3121 tgagagacag tatgaatacc gaagtaaaaa gcgggttgaa agagggggac aaagtggtca
     3181 tctccgaaat aaccgccgcc gagcagcagg aaagcggcga acgcgcccta ggcggcccgc
     3241 cgcgccgata aacgaatatg ccgtctgaac acggaaacgg tttcagacgg catttgttat
     3301 tgatttacgg aatattatga gcttgatcga atgtaaaaac atcaaccgct atttcggcag
     3361 cggcgagaac cgcgtccata ttttgaaaga catcagcctg tcgatagaga agggcgattt
     3421 tgtcgccatc atcgggcagt ccggttcggg caagtccacg ctgatgaaca tactcggctg
     3481 tttggatacc gccggttccg gttcgtaccg aatcgacgac atcgaaactg ccaaaatgca
     3541 gcctgacgag ctggcggcat tgcgccgcga acgcttcggt ttcatcttcc aacgctacaa
     3601 cctcttaagc tcgctgaccg caagggacaa cgtcgcgctg ccagccgtct atatgggcgc
     3661 gggcggcaaa gagcgttccg cgcgtgcgga gaagttgttg caggatttgg gtttggcaag
     3721 caaagagggc aacaagcccg gcgaactctc gggcggacag cagcagcgcg tctccatcgc
     3781 ccgcgccctg atgaacggcg gagaaatcat cttcgccgac gagccgaccg gcgcactcga
     3841 taccgccagc ggcaaaaacg tgatggaaat catccacaag ctgcacgaag ccgggcatac
     3901 tgtcattatg gtcacgcacg accccggcat cgccgccaat gccaaccgcg tcatcgaaat
     3961 ccgggacggc gaaatcattt ccgacacctc gaaaaatccc gaaatccccg caagcaatgt
     4021 cgggaggatt cgggaaaaag cctcgtggtc gttttattac gaccagtttg tcgaagcctt
     4081 cagaatgtcg gtgcaagcag tattggcgca caaaatgcgt tcgcttctga cgatgctcgg
     4141 catcatcatc ggcatcgcgt cggtggtttc cgtcgtcgca ttgggcaacg gttcgcagaa
     4201 aaaaatcctt gaagacatca gttcgatagg gacgaacacc atcagcatct tcccggggcg
     4261 cggcttcggc gacaggcgca gcggcaggat taaaaccctg accatagacg acgcaaaaat
     4321 catcgccaaa caaagctacg ttgcttccgc cacgcccatg acttcgagcg gcggcacgct
     4381 gacttaccgc aataccgacc tgaccgcttc tttgtacggc gtgggcgaac aatatttcga
     4441 cgtgcgcgga ctgaagctgg aaacggggcg gctgtttgac gaaaacgatg tgaaagaaga
     4501 cgcgcaggtc gtcgtcatcg accaaaatgt caaagacaaa ctctttgcgg actcggatcc
     4561 gttgggtaaa accattttgt tcaggaaacg ccccttgacc gtcatcggcg tgatgaaaaa
     4621 agacgaaaac gctttcggca attccgacgt gctgatgctt tggtcgccct atacgacggt
     4681 gatgcaccaa atcacaggcg agagccacac caactccatc accgtcaaaa tcaaagacaa
     4741 tgccaatacc caggttgccg aaaaagggct gaccgatctg ctcaaagcgc ggcacggcac
     4801 ggaagatttc ttcatgaaca acagcgacag catcaggcag atagtcgaaa gcaccaccgg
     4861 tacgatgaag ctgctgattt cctccatcgc cctgatttca ttggtagtcg gcggcatcgg
     4921 cgtgatgaac atcatgctgg tgtccgttac cgagcgcacc aaagaaatcg gcatacggat
     4981 ggcaatcggc gcgcggcgcg gcaatatctt gcagcagttt ttgattgagg cggtgttaat
     5041 ctgcgtcatc ggcggtttgg tcggcgtggg tttgtccgcc gccgtcagcc tcgtgttcaa
     5101 tcattttgta accgacttcc cgatggacat ttcggcggca tccgttatcg gggcggtcgc
     5161 ctgttcgacc ggaatcggca tcgcgttcgg ctttatgcct gccaataaag cagccaaact
     5221 caatccgata gacgcattgg cgcaggattg aggttggcac aaagatgccg tctgaagctg
     5281 caggaccggt cattttggag cagaaactta ttggacaaaa acggtttctt agattctacg
     5341 ttctagattc ccacttgcgt gggaatgacg gcggtgtgtt tcgtattgtt cagtttatta
     5401 ttttcaatca ttcaatgggt taggatgtgt ttgttggctt gctgtactgg tttaaattta
     5461 atccactata aatttaatcc actataactt tcagtgcgga ttggttttca gacggcattt
     5521 cccgcaaaaa aggcttggaa ttttccaagc cttttgtttt gcggatggat tattgatttt
     5581 tgcggatgat ttcctccagt tggggcatcg ggctgtagcc gctttggctg cgcccgttgg
     5641 ggaagacgag ggtcggcgtg ccgttgaagc cgaattgttc gcccaaggaa gtggtttccg
     5701 cgacgggatt gtcgcagatg ctgccgccga ccgggaattt gcctttacgc atccaatccg
     5761 tccacgcttt ggcgcggtcg ggctgacacc ataagatttg cgccttgcgc gcggcatcgg
     5821 ggtgcaggcc ggcaatgggc atcataaagc tgtaaaccgt cacgtcggtc attttttcaa
     5881 actcgtgttc caagcgtttg cagaacggac aatcggggtc ggagaagacg gcgactttca
     5941 gcttgccgtt gccgcgcact tctttgatgg ctttgtccaa aggcagggag gcgaagtcga
     6001 ttttgttcaa atcggcggcg cgttcttcgg tcaggttttt gcgcgtgtcg atgttgatga
     6061 gttcgccgac gaacatatag ccgccttcgg catcggtgta gataatctgc ctgccgctga
     6121 cgacgacttc gtaaatgcct ttgaccggtg tttcgctgac gctcaacact ttcaaatctt
     6181 gggcggaata ggttttttcc aaacgcgctt tcaaagaggc ggcaacggat ttgccggcgg
     6241 actcggcttt gacggcgggt tcggcgttgg cattggaaac gggcgtttgc ccgcaagcca
     6301 gcagtgggag gacggtaaag ggggtcaaga ttttgattaa cttggttttc atataaagat
     6361 gattgcgcgt gttggaaaag cggaattgta tcaaatctct gttgcgcctg cattgcgcct
     6421 aggctcaatt tatcgtctga aaatagcttc cgcctgttaa aatacgcaaa aaaatgattt
     6481 gcctgcctgt atgatctacc accgcatcgc cgtaaacgtg ccgctttcag acggcctttt
     6541 gacttattcc cattccgatc cgcttcctcc gggaacgcgg gtgcttgtgc ctttccgcaa
     6601 taaaaccgtg gtcgggatgg tgtgggaaac ggatattgcg ccggatatgg atacggcgcg
     6661 gattttgagt attcagacga cctttttaga cgagccgccg ttgcctgaaa gctggcgtga
     6721 tttgctctcg tttacgtcgc gttattacca ctatccgacg gggcaggcgg tgtttgccgc
     6781 gctgccgcag ggtttgaagg aaacgcgcgc ggtggaaatg ccgcagccgc cgttgtttta
     6841 cgcgctgaac gaagcgggca gggcgcaaac gccgccgctg gcgcggttca acaaaaaagc
     6901 ggctctgtgg gacgcactgc tgtcgggcgg gatgacgatg gcggcgttga agcaggtaaa
     6961 cgcgcaggcg gcgaaattga ttgaggattg ggctgaacaa ggctggattg aaacgacgga
     7021 agcggcgaag cccgttttaa ggtcgtgtaa tgggcaggct tcgcactctg aatttgtatt
     7081 aaatgctgac cagcagaagg cttccgatgc cattcagacg gcattgggac gcttccagcc
     7141 gtttctgctg tacggcatca ccggcagcgg caagaccgag gtgtattttg atgcgatggc
     7201 gaaagtgttg gctcaggggc ggcaggtgtt gtttctgttg cccgaaatca acctcacgcc
     7261 gcagcttttg aagcgggtgg aaaaccgttt tgccgacgtg ccgaccgccg tgttgcacag
     7321 tcagatggcg gcaggcaagc gcacgcagga ttatctgcgc gcgatgttag ggcaggcgaa
     7381 gctggtgatc ggcacgcggc tggcagtgtt cacgccgatg gatgatgtcg ggctgattgt
     7441 ggtcgatgag gaacacgacg gctcgttcaa acaagacaac gaattgcgct atcatgcccg
     7501 cgatttggcg gtgtggcggg cgaagcaggg cggctgcccc gtcgtgttgg gcagtgccac
     7561 gcccagcttg gagagctggc acaaggcgca aagtggcgcg taccgcctgc tgcaactgac
     7621 cgaacgcgcc cataccgccg cgcaactgcc gcaagtggac attctcaacg taggccgtct
     7681 gaaactcgac aacggcttct cgccgcaagc cttgcagctt ttgaaacaga actttgaagc
     7741 aggcggtatg tcgctggtgt accttaaccg gcgcggcttc gcgcccgcgc tgttttgcgg
     7801 cgactgcggt cataccttcg gctgcccgaa ctgctccgcc aaaatggtgc tgcaccaacg
     7861 cgcccgccaa ctgcgctgcc accactgcga ccaccgcgaa cccatcccgt acaaatgccc
     7921 cgactgcggc aaccaagacc tgaccgccgt cggccacggc acgcagcgcg tcgaagaaac
     7981 cctgcgcgcc ttcctgccca aggcagccgt cgtccgcgtc gatagggaca gtacggcgca
     8041 caaaaacgac tgggcggatt tgtaccgccg catcgccgac aacgaaatcg atattctggt
     8101 cggcacgcag atgctcgcca aaggtcatga tttcgcgcgg ctcaacctcg ttatcgtatt
     8161 gaacgccgac ggcagcctgt acagcgcgga tttccgcgcg ccggaaaggc tgtttgccga
     8221 gctgatgcag gtgtccggca gggcggggcg cgccgacaag cccggcaagg tgctgataca
     8281 gacccaactg cccgaacatc ccgtcttcgc cgccgtcaaa gcgcaggact acgtcgtgtt
     8341 tgccgaaaac gaattgaacg aacggcaaat gttcgccatg ccgcccttcg gttttcagac
     8401 cgccgtccgc gcggacgcgc cgcgtgttgc cgatgcgctg gaatttctca atgccgccaa
     8461 agaaaccctc gccccgctgt tgcccgcaag cgtttcccag tttggcgccg cccccatgct
     8521 gatggtgcgc ctcgccgaac gcgaacgcgc gcaaattttc ctcgaatccc cgtcccgaca
     8581 ggatttgcac cgtgccgtga gtttgtgggt gcaggtgttg cagcaaaacc gcgacggcaa
     8641 aattagatgg tcggtggatg tcgatccgca ggaggcttga ttatcggcaa tacgatgccg
     8701 tctgaaaccg tttcagacgg ccttggattc ggatttcaag tgcaa
//
LOCUS       DAXHKX010000074         8741 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHKX010000074 DAXHKX010000000
VERSION     DAXHKX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.074"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(126..338)
                     /locus_tag="ACFMWZ_001249"
     CDS             complement(126..338)
                     /locus_tag="ACFMWZ_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC5935214.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(350..631)
                     /locus_tag="ACFMWZ_001250"
     CDS             complement(350..631)
                     /locus_tag="ACFMWZ_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935215.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(669..872)
                     /locus_tag="ACFMWZ_001251"
     CDS             complement(669..872)
                     /locus_tag="ACFMWZ_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935216.1"
                     /translation="MNIIEQEMKHSSLRKVDVEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFS"
     gene            complement(952..1524)
                     /locus_tag="ACFMWZ_001252"
     CDS             complement(952..1524)
                     /locus_tag="ACFMWZ_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC5935217.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            complement(1499..1843)
                     /locus_tag="ACFMWZ_001253"
                     /pseudo
     CDS             complement(1499..1843)
                     /locus_tag="ACFMWZ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1836..2093)
                     /locus_tag="ACFMWZ_001254"
                     /pseudo
     CDS             complement(1836..2093)
                     /locus_tag="ACFMWZ_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2104..2424
                     /locus_tag="ACFMWZ_001255"
     CDS             2104..2424
                     /locus_tag="ACFMWZ_001255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935218.1"
                     /translation="MLGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDL
                     TGKKKETSSAKKFVMPCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENF
                     RQYE"
     gene            2456..2611
                     /locus_tag="ACFMWZ_001256"
     CDS             2456..2611
                     /locus_tag="ACFMWZ_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC5935219.1"
                     /translation="MLWVKRIQRQIDGSLLLISDNSTYPPMPLALAEHPDIQIIGQVV
                     QVSKDLN"
     gene            2635..3195
                     /locus_tag="ACFMWZ_001257"
     CDS             2635..3195
                     /locus_tag="ACFMWZ_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935220.1"
                     /translation="MKILALLIAATCALSACGGQAEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSVANKGLADQKTGLTLPEHVVPADNAEGKNLLHDFSDGLTLTVDTDKADKITA
                     VRVVWNTDAVPQKAEKLSKAAAALIAATAPGDRTLLRDTGDQIKMAIDSHNAQKEPTR
                     EWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            3336..4394
                     /locus_tag="ACFMWZ_001258"
     CDS             3336..4394
                     /locus_tag="ACFMWZ_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935221.1"
                     /translation="MKNKLTLRYFKITRCGYWSGSSKEPNHYEDLLKTLTHLETYVKG
                     KTIEETGISRDDKEPSTFLLDICKKNGLWLLAFWNPAAESSSDEIVSLNMKSKLGQVD
                     SSPVTAGNGKTFGFVSYFLIWPEHNFYSTILSPNATLAGRTEFEEYMLNFLWIDPNFI
                     EKTTYEETSTDGIIHTMERTNFKLPLKINGTPVPRFAGKLAKSPSEKEYVISNYDQVT
                     QVHNQCTVKVTSGQSSSGILKKMLPSFFGSTELFAPLKTIRVNHSVSAAFENKSNLKD
                     WIEDWEKNSFDLDKDDCGFKLKNDDKIYWIRGNVIKCELDVNLHKENGIYPAAKILDI
                     IDQRKDYVLNEFKLSDDS"
     gene            4384..4890
                     /locus_tag="ACFMWZ_001259"
     CDS             4384..4890
                     /locus_tag="ACFMWZ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935222.1"
                     /translation="MILRNIFLICSAFMIGVAWYLGANIRFEEQWVLYEALRTTASII
                     FAVVGVWLAIVYPERLKSPFSQTFSSPVYRRGFKQLFSPVISSIFILSIVLLVGLLAP
                     LLKHIEAIIQYIPLCRKISFSLLASLTMWQIYTVLSVLVPLLNILSKNENDCHASNII
                     NKLKGEQN"
     gene            4994..5497
                     /locus_tag="ACFMWZ_001260"
     CDS             4994..5497
                     /locus_tag="ACFMWZ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC5935223.1"
                     /translation="MSTKFNQFINRVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            5609..5941
                     /locus_tag="ACFMWZ_001261"
     CDS             5609..5941
                     /locus_tag="ACFMWZ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935224.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            5938..6348
                     /locus_tag="ACFMWZ_001262"
     CDS             5938..6348
                     /locus_tag="ACFMWZ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC5935225.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            6353..6901
                     /locus_tag="ACFMWZ_001263"
                     /pseudo
     CDS             6353..6901
                     /locus_tag="ACFMWZ_001263"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6917..7102
                     /locus_tag="ACFMWZ_001264"
                     /pseudo
     CDS             6917..7102
                     /locus_tag="ACFMWZ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(7421..7783)
                     /gene="ssrA"
                     /locus_tag="ACFMWZ_001265"
     tmRNA           complement(7421..7783)
                     /gene="ssrA"
                     /locus_tag="ACFMWZ_001265"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(7846..8451)
                     /locus_tag="ACFMWZ_001266"
     CDS             complement(7846..8451)
                     /locus_tag="ACFMWZ_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC5935226.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            complement(8495..>8741)
                     /locus_tag="ACFMWZ_001267"
     CDS             complement(8495..>8741)
                     /locus_tag="ACFMWZ_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935227.1"
                     /translation="SDFRVSRFRQFKIGKTRQPASQGRFLKLYQAFGHIGSTGPHFRQ
                     STLPTVAAFLFDIQSHFQKTYAGNKIILPRLANIPAN"
BASE COUNT         2198 a         1928 c         2231 g         2384 t
ORIGIN      
        1 agtgagttta ttttggggcg gcggcaggtc gggggcaagc ggcgtggggc ttggttgtgg
       61 tttttaggtt tgggggtggg taaaaaatgc cgtacccggc gggcaggctt cgcactctga
      121 aggtttcaga cggcgtttgt tttttctgtc caatcgagga actgccgcca tttttccagc
      181 ggcatatcgg cccgccgcgt ttcggtatcg ggttcggctt cccagcggcc tacctgtatg
      241 tagtgcttca ccccgacgat ttgcgccaac tcggcctgtg tcagtttgca gcggttgcgc
      301 agggtgcgaa ggttgtaagg cgtgtagccg agttccatgt cgttgcggtt tattttgttt
      361 cgcatatttt tttgactgcc cggcggcagg tttcggtaag gacggcggca aatcgggctt
      421 cgtctgccgg ttttccgtcg aaaaaaataa aatcgtaaag ggtgatgccg ttttcggaat
      481 ggcggtaatg cccgcccggg cattcgccgt ctgtctgcct gtggattttg gcaatcaggc
      541 ggggatagcg gcaatgggta atgaaacggc ttgcgccgtc ttgcccgata atccattcgg
      601 ggtagcggtt gaatagtgcg gctctgttca ttttgttcgg gggataaagc ccctcgcggg
      661 gcttgtggtc aggaaaattt gaatatcagt gccaacacgg cggcgatggc ggtaaccagc
      721 ccggtggccg ccatcatggg ataccatcgg gattcttggg ctatttttac ggattcggcg
      781 ttgattttgt gcgcgtctgc aatgattttg gcgatttcga catctacttt tctcagactg
      841 gagtgtttca tttcttgttc gattatgttc atcgtacttc ctttcgtttt tggcggttgc
      901 cgccgcttgt cggatggcag gatgtctgcc atgtgtatat attgatacct tttaggtttt
      961 attgcaagtg ttttgggcgg cggcttcgta tgcttggcgg tggcggcggc tgtaccattc
     1021 ggcgagttcg cgccgctctt ggaggcggta gctgtcggcg gtcaggtagt ggtgcaggct
     1081 tgagaagccg gcgcgttgga gggcggcgcg gctgatgtgg ccgagggcga ggtctgttgt
     1141 gagggtgtct tttccggcgg tcaggctgat gttggtgaag aggtggcgga atccgtgcat
     1201 tgtgtgtttg gatttgccgg gggtgctgcc gtcatagccc agtcgtcgga tggcgttgtg
     1261 ggcgaatttg atgctgatgt ggtcgggatg gggggcgggt ttgcgccgtg ggcggatgcc
     1321 ggggaaaagg tggatgttgt cgccggtctg tgtgtgcagc tctcggagta tttctaccgc
     1381 ccagtccgac agtgggacgg taaaggggtg tttggtcttc atgtcggcgg cggggatgtg
     1441 ccataaccgg gcggtgaggt cgatgtcttg ccagcgggcg gaaagcagtg cggacggacg
     1501 ggatgacttc cccggtttcc ccatcaatcc gcgccgcctc gatttttccg ttggcgcgca
     1561 gcatttccac cgcctgccgc accgacatca accgcttgcc gttgcgcctc atcgcctcca
     1621 ccaaaaccgc cgaaatcaat ttggcttctt ccgttttaag ctccgtcttg cccgcatcgc
     1681 tgcgccgttt gcgcgtcggc ttgatgctga ccgcctccag cttgcggtat agcgtggcaa
     1741 ggctgatgcc caattcctgc gcctgctgct taagatatgc agagcgtgcg ccgcgtccca
     1801 ttgcttccgc ctgattctcg actgccttaa gacgctcaat cattgccgga ttcatcgcct
     1861 tctcccgttt caccgcccaa ccattccggc acattgtctg tcggtgcttc agtcggcagg
     1921 gcatagcttt cgcgcagttg ctcgcagtcc aaaataattt gattgagcgt gccgaccatc
     1981 tttgcctgat ggctgatctc gtgtgcctca ctgtgcgcat taagttggtc gaacaaatct
     2041 ttcaaacggc tcacttgact gcggataccg acctcaaggc ttgttaactg catcatttat
     2101 gatttgttag gggttttaga ctgctatcaa aaatcacagt cctgtcccaa ccctaaaagc
     2161 cactgttgct ttgtttcaga cgacattttt ctatggcatg gatcaagacc aacgtttgaa
     2221 atcagtcttg atctaactgg aaagaaaaaa gaaacatcct ctgcaaagaa atttgtgatg
     2281 ccttgtatta atttccgtca ccataggatt gaaaaagaaa cctacttagg aaattggaat
     2341 gaacaaattg ccgcattggc agtaaaacaa aatatagatt ggtgtccaag ttttgatatt
     2401 gagaatttta gacagtatga ataattacta tctatatagg aattgcagca gcgatgtgtt
     2461 atgggtcaaa cgtatccaac gccaaatcga cggcagccta ctcttgattt ctgacaattc
     2521 aacctatcca cccatgccct tggcactggc ggaacacccc gatattcaaa tcatcgggca
     2581 ggtagtgcag gtatcaaaag acttgaacta gacacaatca aaaagggaaa tagaatgaaa
     2641 atactcgctt tattaattgc cgctacctgt gctttatctg cgtgtggcgg ccaagctgaa
     2701 gaacaaccgg catctgccca acctcaagag caggcacaat ccgaattaaa aaccatgccg
     2761 gtaagctata ccgactatca atcagtagcc aataaagggc ttgccgacca aaaaactggt
     2821 ctgacccttc cagaacatgt tgttcctgcc gacaatgcgg aagggaagaa tctgctgcat
     2881 gacttttcag acggcctcac attaaccgtt gataccgata aagccgacaa aattactgcc
     2941 gtccgagtag tttggaatac agatgcagtg cctcaaaaag cggaaaaact gtccaaagcc
     3001 gccgcagcct tgattgcggc aaccgcccca ggagaccgca cactgctgcg tgataccggc
     3061 gaccaaatca aaatggcaat tgacagccat aatgcgcaaa aagagccaac ccgagaatgg
     3121 gcgcgtggtg ggattgctta taaagtcact gttactaatt tgccgagcgt ggttttgaca
     3181 gcaaaagctg aataacctgc attacagcca caattaggag cagacggaga tgatgtttat
     3241 agagcctgct gagaataaat aactgataaa aaagctgtct gaaaaggttt cggatagtta
     3301 ggatagctat ctatttttaa tatattagag agaatatgaa aaataaactg actttacgtt
     3361 atttcaaaat aactcgttgc ggttattggt ctggttcttc aaaagaacct aatcattatg
     3421 aggacttatt aaaaacttta actcatttag agacatatgt taaaggtaaa actattgaag
     3481 agactggaat tagtcgtgat gataaagagc caagcacttt cttgctcgat atctgcaaaa
     3541 aaaatggtct gtggttgctt gctttttgga atcctgccgc agaaagctct tcagatgaaa
     3601 ttgtttcttt aaacatgaaa agtaaactag gtcaagttga ttctagccca gtaacagcag
     3661 gaaatgggaa aacttttggc ttcgttagtt attttcttat ttggccagaa cataattttt
     3721 actcaacgat tctttctcct aatgcaactt tagctggacg aacagagttt gaagagtaca
     3781 tgcttaattt cttgtggatt gatcctaatt ttatagaaaa gactacctac gaagaaactt
     3841 caacagacgg aataattcat accatggagc gaactaattt taaattgcct cttaaaatta
     3901 atggaactcc tgtgccacgt tttgcgggga aactagctaa atctccttca gaaaaagagt
     3961 atgtcattag taattatgac caagtaaccc aagtgcataa tcaatgcacg gtaaaggtta
     4021 cctctggaca aagttcaagt ggaattttga agaaaatgtt accaagcttt tttggttcaa
     4081 cagaattgtt tgctccatta aaaaccattc gtgtaaatca ttctgtttct gctgcatttg
     4141 aaaacaaatc aaatcttaag gattggattg aagactggga aaaaaattct tttgatttag
     4201 ataaagatga ttgtggcttt aagttaaaaa acgatgacaa gatttattgg attcgaggaa
     4261 atgtcatcaa atgtgaatta gacgtcaacc ttcataagga aaatgggatt tatccagctg
     4321 ctaaaattct agatataata gaccaaagga aagattacgt tttaaacgaa ttcaaattaa
     4381 gtgatgattc ttagaaatat tttcctaata tgctctgcat tcatgattgg tgtagcctgg
     4441 tatttaggtg ctaatatccg atttgaagag caatgggtat tgtatgaagc cttgagaaca
     4501 actgcatcaa ttatttttgc agttgttggg gtttggttgg ctattgtcta tcccgaacga
     4561 ttaaaaagcc cttttagtca aactttctcc tctccggtat atcggcgtgg attcaaacag
     4621 cttttttccc ctgttataag ttccattttt attttaagta ttgtgttact tgttggtttg
     4681 ttggctcctt tgctcaaaca tattgaggca ataattcaat atatcccact ttgtcggaaa
     4741 atctcattta gcctgttagc aagcttgacg atgtggcaaa tttatacggt tttatctgta
     4801 ttagtgcctt tgttaaatat tttaagtaaa aatgaaaatg actgtcatgc atccaacatt
     4861 attaacaagc taaagggtga acagaattag ctatgtgatt tttagtgtaa actgctttta
     4921 actcgtatta aaaggccact cttttaatac ctcctaaaat ccctgtacct atcaatagat
     4981 acagggattt tttatgtcca ccaaattcaa ccaattcatc aaccgcgtcc tctcccacga
     5041 gggcggttac gtcaatcatc ccaaagaccc cggcggcgaa accaattggg gcatcactaa
     5101 gcgcaccgca caggcaaacg gctacaacgg ctccatgcgc gccatgacgc gtgaacaggc
     5161 tatcggcatt taccgaaagg cgttttggga gcgttaccgc gccgaccaaa tgccggaagc
     5221 ggtcgcgttc caattttttg atgcctgcgt caaccacggt tacggcaatg ccgtccgtat
     5281 gctgcaacgc gccgcaggcg tggcggacga tggaatcatc ggcgaaatca gcctcaaagc
     5341 catcaattcc ctccccgaaa acgacctttt attgcggttc aacgccgagc gtctggtctt
     5401 ttataccaag ctcagtacgt tcacctcttt cggcaagggc tgggtacgcc gtgtggcgca
     5461 aaacctgatt cacgcgtctg cagataacac tgattaaagg gagacaaacc atgtcaaaaa
     5521 agtcactcat cgccctaatg accgcagcca tgcagcccga tttcagccac agcgacctag
     5581 gcattcgcta cgccatgccg actcagggat gttggacgca agcccaccgc aagagcgggg
     5641 tagccgccgc gaaacgcgca gccaaaaaaa agcgtcgcaa ataactgcct ttttccgatg
     5701 gctgggcggc ttggtctcta atccggccac aggaaaaatc agccatacca aactttgggc
     5761 aaacgtcgcc gcagccgcca tgacttggaa gttcgtgcag gcggcggacg cgcccgaatg
     5821 gctttggtgg gcatacggcg cgatggtcgg cgggtatgca ttaatcaaac gcggcatcgc
     5881 cgtcgtaccg cagttggcgg aagtcaaaaa atccgcaaat caggaagggg ggcggcaatg
     5941 attgaatttg tccgagccaa aaaacggctg ctttgggcat ttgtgctttt gcttgtgtgg
     6001 acgtgcggtt accgatacgc cgccgacaag gccgaagcgg aacaaaccgc cctgattgcc
     6061 acctatcggc attcttctat ggttgcggcg gaacaatatg ccttgcagct taaaaaagcg
     6121 caggacgaaa ggcagcggtg gtacgacttt tcccaaaaac aaggaagaaa gcccgtgaaa
     6181 aaacagtatc cgccgcaaac gaaaaaagcc ggctatctga aaaccaagga agaactgctt
     6241 gcggaattgg cttgccttac agcggaaatg gctgccctaa aaaagcccga tgccttaatc
     6301 catgggaaag aagtgcggca gaaagaacgc aactcgtcgc agggttaagg caatgccatc
     6361 cgttgaactg ctgttggaga ttgtcggact gccacgcggc accttttatt accaattggc
     6421 cgtccaatcg gcagaagaca aatatgccga tttgaaacag catatccatg atatttatcg
     6481 acgacataag ggaagatacg gctaccggag gattgcagca gccatccttc atgcaggaac
     6541 accggtcaat cacaagaaag tcagccgtct gatggcgaag acggggctga aggcagtgat
     6601 acggcggcgc aaataccgct cgttcaaagg agaagtcggc aaaattgcgc cgaatatcct
     6661 gcgacgctgt ttccatgcag aaaagccgaa tgagaaatgg gtaacggacg ttaccgagtt
     6721 caatgtaggc ggagaaaaga tatacctttc tccgattatg gatttgttta acggggaaat
     6781 cgtcagttac cgtattcaaa tccgcccgac tttcgatttg gccggcgaga tactgaaagg
     6841 tgcgccggag aaaccgggat cgtctgaaaa gccgatactg cattcggatc aaggttggca
     6901 atatcagatg tttttttata gtggattaaa tttaaatcag gacaaggcga tgaagccgca
     6961 gacagtacaa atagtacggc aaggcgaggc aacgccgtac tggtttaaat ttaatccact
     7021 atacaacaac gatagaatca agttgaaatt aaaagggctg agccttgttc agtacagaat
     7081 tcagtccctg aaagccgctt gattaaactg tccgactttt tggggtcagt tcggcttcgg
     7141 cattttttta tccgtttggg ggtaacttgt ttggaaagct gcaagcttat aaataaagga
     7201 ttacatttaa gttttgggta acctttttaa aaaaatgcgt gatgactttt gcatttttaa
     7261 ggcgtttttt ggggtaattc gtgaaaagtt accccaaaag ttaccccata aatggcgaaa
     7321 actcaagcat acgccagcat attcaaggca acaaaaaagc cttgaaactg ttgaagttca
     7381 aggctttttt gtgttgcagg atgctgctgg aaatagggta tggtggaggc ggggggaatc
     7441 gaacccccgt ccgaaagtcc tctacaaagc gttctacata cttagttgtg tctatttgaa
     7501 aatcttattt ccatcatgcc gaccaacagg ccttttggaa accagttacc ttaaatctca
     7561 tttcctgcca agtaacccgg taggaaacca gtcaatgtaa gatgacgttg cggtggcttt
     7621 cgccacacag cccattgacc gactgctgca acggctagcc ttaagcggct aaagcgtaag
     7681 tttcgtcgtt tgcgactatt tgaattcagt gttttacggg aatctgagac cccggtatgc
     7741 ccgcatctgc ttcgcaaccc ccgtcgaaac caaggtcgcc cccagaaatg gtttgcaaat
     7801 tatacggata ttgtgcggtg ctgccaagtc tgtcggagaa atttgtcagt cttgctgcct
     7861 taatttgcgt ttgagcagga tgcggacgca gccgtcgttg ccttcccggg gttcggcgta
     7921 ggcgagtacg tcggggtgtt gcatcagcca gtttcgggtc atatttttca gaacgggttt
     7981 gtagcctttg gaacctaatc cgctgccgtg gatgatttcg ccgcatacgc cgcgtttttg
     8041 ggtgaatgcg atgaattcgt tgaggacttt ttgggcttct tcctgtgtgt agccgtgcag
     8101 gtcgacatcg gtaacgacgg gatagtatcc gtttttcagg cgttggatgt cgttttttcc
     8161 ctgtccgttt ttgctgaagc tggcgggcgg gtcgttgtat gtgctgccga tgtagaagta
     8221 gttttcttcg tcggcgcggt tgtctttggg acggactttg atgggggttt tgtcgggcgg
     8281 cgcataatat tgctgccggt tttttaatgg ggagacttgt ccgactgctt gtgaaaaatc
     8341 gaaatcctgt tcttgttttt gcttgttttt ttcggctgcc tgtttttctg ccgcttcttt
     8401 ttgggcttgt ttgcccagtt gtttgaggat gttttggaag tcggtattca tattttttcc
     8461 tgttatttgt ccgatggctg tttcgggcgg ggttttaatt tgccggaatg tttgccaatc
     8521 gggggaggat gattttgttg cctgcgtatg ttttttgaaa gtgtgattgt atatcaaaaa
     8581 gaaatgcggc aaccgtcggc agtgttgatt gccggaaatg cggaccggtc gaaccgatat
     8641 gcccgaacgc ctgataaagt tttaaaaacc tgccttgcga agcaggctga cgtgttttgc
     8701 caatcttgaa ttgccggaaa cgcgaaacac ggaaatctga t
//
LOCUS       DAXHKX010000075         8597 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHKX010000075 DAXHKX010000000
VERSION     DAXHKX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.075"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(236..3088)
                     /gene="gcvP"
                     /locus_tag="ACFMWZ_001268"
     CDS             complement(236..3088)
                     /gene="gcvP"
                     /locus_tag="ACFMWZ_001268"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC5935228.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLETLGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFDFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKPPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIAKRIHALASAFADALVSDGIKVVHKVFFDTVTVDFGSK
                     EKTDQVFAAALESGYNLRRVNDTQVAAAFHETSTREDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQNDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3119..3478)
                     /locus_tag="ACFMWZ_001269"
     CDS             complement(3119..3478)
                     /locus_tag="ACFMWZ_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935229.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCTNHPMSKNSA"
     gene            3477..3656
                     /locus_tag="ACFMWZ_001270"
     CDS             3477..3656
                     /locus_tag="ACFMWZ_001270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC5935230.1"
                     /translation="MPSEPAGRLFQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3649..4290)
                     /locus_tag="ACFMWZ_001271"
     CDS             complement(3649..4290)
                     /locus_tag="ACFMWZ_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC5935231.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGKKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEAYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4280..4831)
                     /locus_tag="ACFMWZ_001272"
     CDS             complement(4280..4831)
                     /locus_tag="ACFMWZ_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC5935232.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRNTLKYHPKWLDAV"
     gene            complement(4835..5353)
                     /locus_tag="ACFMWZ_001273"
     CDS             complement(4835..5353)
                     /locus_tag="ACFMWZ_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC5935233.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGSETAVK
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5353..7014)
                     /gene="pqiB"
                     /locus_tag="ACFMWZ_001274"
     CDS             complement(5353..7014)
                     /gene="pqiB"
                     /locus_tag="ACFMWZ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC5935234.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLAGNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7007..8311)
                     /locus_tag="ACFMWZ_001275"
     CDS             complement(7007..8311)
                     /locus_tag="ACFMWZ_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC5935235.1"
                     /translation="MKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPQLNRGE
                     AAFCPRCGYKLFRVGRHPFSAPPAYAATSLILMAFAYGMTYIEVGIPGAASVLSLPEM
                     MRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLR
                     QAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIG
                     RLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAV
                     ILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAA
                     MSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGS
                     AAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1914 a         2203 c         2440 g         2040 t
ORIGIN      
        1 tttgtccgtg tggaaatgac atcaaagggt gtgaataggc ttttcatctt tgaaatctgt
       61 ttgcacgatc ggatgaataa aaagtaggat gataaaaaag ctgatgtatg gatactggtt
      121 taactcattt gttttaaagt tatagtcgag atacgtgtat tggtgcaact ggtttgccgt
      181 tgatgaaaat gccgtctgaa aatgtttcag acggcatttt ttaagatatc aacagtcagt
      241 cttcataatt ttccatcggt gggcagctgc acacgagatt gcggtcgccg tacacgtcgt
      301 ccacgcggtt gacgaagggc caaaacttgt gttcgcggac gaacggcaac gggaagacgg
      361 cttcttcgcg ggaatacgga tgcgcccagt tgccggttat atctgcggcg gtgtgcggcg
      421 cgttgaccag tgggttgtcg tctttcggcc attcgccgcg cccgactttc agcacttcct
      481 gtttgatttg tttcagggcg gcgatgaagc ggtcgagttc ggctttgctt tcgctctcgg
      541 tcggctcgat catcagcgta ccggcaacgg ggaaggaaac cgtcggggcg tggaagccgt
      601 agtccatcag gcgtttggcg atgtcggttt cggtaatgcc gctttcggct ttgagcggac
      661 gcaaatcgac gatacattcg tgcgcgacgc ggccgttttt gcctgtatac aggatcggat
      721 agtcttcgcc cagccgtttg gcaacgtagt tggcgttgag caatgcccag cgtgtcgcct
      781 gttccatgcc ttgtttgccc atcatggtca gatacatcca agtaatcggc agtatggacg
      841 cagaaccgaa ggccgcagca gcaacggcgg tttggtcggc actggcgctg tgggtgtcgg
      901 tcaaagcgtg tcccggtgcg aacggagcca gatgggcttt caagccaatc ggacccatgc
      961 ccggaccgcc gccgccgtga gggatacaga aggttttgtg caggttcatg tgcaacacgt
     1021 ccgcgccgac ttcggcaggc tgcatgatgc cgatttgggc gttgaggttg gcgccgtcca
     1081 tgtaaacctg tccgccgttt tcatggacga tgcggcagat gtcgcggatg ccttcttcgt
     1141 acacgccgtg ggtggacgga taggtaatca tgatggcgga caaagcgtcg cggtgttgct
     1201 cggctttggc tttcaaatcg tcgatgttga cgttgccgtg ttcgtcggta tcgacgacga
     1261 cgactttcaa accgagcatg gcggcggtgg cggggttggt gccgtgggcg gatttgggaa
     1321 tcagacagat gttgcggtgt ccctcgccgt tggcttcctg atagcggcgg atggagagca
     1381 taccgctgta ttcgccctgc gcgccggagt tgggctggaa ggaaatcgcg tcaaagccgg
     1441 tgatggcttt caggctgttt tccatgtcgg caagcaattc gcggtagccg gcggtttgcg
     1501 cttcgggagc gtaagggtgg atgtcggaga actccgccca agtaatcggc aacatttccg
     1561 cagtggcatt gagtttcatg gtgcaactgc ccagcgaaat catactgcgg ttcatcgcca
     1621 aatcgcggtc ttcgagtttt ttcaggtagc gcaacatttc gtgttcggtg tggtaactgt
     1681 tgaacacagg atgttgcaga atgtcgtttt gacgcagtaa ttcggcgttc agacggcctt
     1741 tgacatcatc ggcaaatgcg aacgtatcct tgccggtaaa cgcgcggtac aaatcaacca
     1801 aatcttcgcg tgtcgaagtt tcatggaagg ctgctgcgac ttgagtatcg ttgacgcggc
     1861 gcaggttgta acccgattcc aaagcagcgg caaacacttg gtctgttttc tctttactgc
     1921 cgaaatcgac ggtaacggta tcgaagaaga ctttgtgaac cactttaatg ccgtctgaaa
     1981 ccagcgcgtc ggcaaaggca gaagccagcg cgtgaatgcg cttggcaata cgtttcacac
     2041 cttcggggcc gtggtaaacg gcatacatac ccgccaagtt cgccagcaat gcctgtgccg
     2101 tacaaatatt ggatgtcgct ttttcgcggc ggatgtgttg ctcgcgggtg gacaacgcca
     2161 tgcgcaaggc aggtttgccc gatgcgtctt tggatacgcc gatgatgcgg cccggggcgg
     2221 aacgtttgaa cgcgtcttta aacgcgaaat aagcagcgtg cggaccaccg aagcccatcg
     2281 gtacgccgaa gcgttgcgtg ttgcccaaag caatatccgc gcccaattcg gcaggcggtt
     2341 tcagcaaaac caagctcata atgtcggcgg aaacggcaac gattgtgcct tttgctttca
     2401 gacggccgat aacgtcctgc aagtcttgca catcgccgtc tttgccgacg tattggaaca
     2461 gcgcgccgaa gtattcgccc tcgtcggctt gagcaaaatc gccgaccacc agttcaaagt
     2521 cgaaatattt ggcgcgggtt ttcatcacgt ccaaagtttg cggatacacg cgcgcatcca
     2581 caaagaaacg ctcggatttc accttgccca cgcggtgcgc catcgccatc gcttcagccg
     2641 cagcagttgc ctcgtccagc aaagacgcgc ccgccacagg gaaaccggtc aaatcgatac
     2701 acacttgttg gaagtttaac aacgcttcca aacgaccctg ggcaatttcc gcctgatacg
     2761 gcgtgtaggc ggtgtaccag cccggatttt ccaacacgtt gcgcaaaatc acgttcggca
     2821 cgcgggtcgg gtaatagcct aaaccgatat aggacttgtt gattacgttt ttcgacgcaa
     2881 tgcctttcaa tttcgccaat gcgtccgcct cggtcaaggc ctcgggcaaa tcgagttcgg
     2941 acggcatacg gatgctttgc ggcacggtat tgccgacaaa atcgtccata ctcttctcgc
     3001 cgagcgtttc caaaagcgcg gcctcgtcgc cgaaactcaa atgtcgcgcg gcaaattcgt
     3061 tggggttgaa cagttcggac agtttcattt ctactccttc gggtttgtag ggctgccatc
     3121 aggcagaatt tttagacatc ggatgattcg tgcaagggta gcacaaaaga cgggataatg
     3181 gcggacaatg cgccagaatg cagcaggtca ggacaagcag cgccacaagc ggcagcgata
     3241 agggaaagcc gccgcccaaa accatgaaac ctgcaggcac cagccaagcc aatggatacg
     3301 ccggaagcgg aaaaacaata ttgagcagaa ccaaaatcac gacaaacagc ggcagacccg
     3361 ccatcaggtt ggcacgcctg tatgttgcgt acgggtcgga tgcttcttca ggttcgcaat
     3421 attccatgat ttcagcctgt ccgggcttta tccacagcag gctcggacgg tcggtcatgc
     3481 cgtctgaacc ggcaggccgt ctttttcaaa aaacgccata ccgctgcaac ttcatgcggc
     3541 tgtatggcgt aggctttgca cttcagacgg catatgcgtc ccgaagcagt ttggtgtccc
     3601 agtcggttca tgattatata aacaacttgg gtgcggataa acctttgttt atctaaaccc
     3661 ctgttttctc aaaaattgat tcaaatcttc ggcaaatttc cgatagcctt tgccgttggc
     3721 gtggatttgg tcggatttca gattattatc gcccaaaatt tccgcccacg cgccgccgaa
     3781 cagcggaatg ccgtatgcct cggacaaatc ctcatacagc ggatggtcgc tcaaatgccc
     3841 gaacaacgcg cccagtgtga tgtgcggcac gccgacgagg acggcgggga tgttttcctt
     3901 ctgcacggtt tcgatgattt tcgcgatatt ggcgcgggtc tgctccttgg gaactttgcg
     3961 cagaaagtcg ttgccgccta tgccgacaat cacaagcttg ggtttgcgtg ccaacagcgc
     4021 gggcaggcgc gacagggctt gcgcggatgt atcgcccgat acgccgccgt tgacaatatt
     4081 ccaacccgtc agtttttgca gttgcgcggg gtaggattcg ccggggtttg cgccgtagcc
     4141 gaaggtgagc gaatcgccca aggcaagtac ggtgcttcct tcgggaattt tggcgtgggt
     4201 tcgggcggat tttttgccgc aggcggtaag cagcaacgcg cccgcgccga ggaggaagat
     4261 tcttctgttc attggttttt cagacggcat cgagccattt cgggtggtat ttcagggtgt
     4321 tacggtaaac cggacagctt tcggcgtgcg cgcctttgag gtagccggta ctcatcagaa
     4381 attcgccgac gatttcgccg ccgacgaatt tgaaatgttt tttaaagagt ttaacccatt
     4441 cgtctttgct tcgcggatgg tgcgtgtcga gccagttctt gaacgagccg tattcttgtt
     4501 gcaacgcttg gatttgccgt gcattgaaaa tggcggcatc gattttcagg cggttgcgga
     4561 caatgcccgc gtcggcaagc aggcgttcgc ggtcggtgtc gtcgaaggcg gcaaccgtat
     4621 cgatgtcgaa accttcaaat gccgtctgaa acgcctgccg cttcttcagc atcagcgtcc
     4681 agtttaatcc tgcctgattg atttccaaca ccagccgctc aaacaattca ttgtcgtcct
     4741 caatcggaaa accgtattgc gtgtcgtggt aatgtttgtt cgggttatcg gtgttttcgg
     4801 gaagtgaggc ggcaaattcg caatagttca cggtttactc gaccatctgt tgcgccgcct
     4861 gtttcagtcc ctgttcgagt gcggcggtca tggcggcgta gccgtcaccc tgctgttcgg
     4921 tttcgatatg gaaaggtctg ttcgtaccgt cgggtaggac ggcgtagccg ctgatgaggg
     4981 ttttgcccgt gtagctgcct tggaatgcgt cgatatagac cgtccatttt tcggtactgc
     5041 cgctgcgtga ggcaggaaca aagatgcgtg tgctgtccaa acggttgaat gcattgctca
     5101 acgccgcttc gagcatatcg tccaaggtgt ctgcccaaac gtggttttgt gcggtgttga
     5161 tgcggtaggg gtcggtttga tagaccagtc cgccgcgttt gagcggctcg gcaagacgga
     5221 ctttgacggc ggtttcgctg ccttgcgttg caggacggat gtagcggctg tcgggcaaca
     5281 cgaaatattg tgtgctttgc acagtaccgc aggcggcaag cgtcagggcg gcggcaatcg
     5341 ggaaaaggcg cattatcggc ttcctttcgg gatggggtct ttgctgctgc tgttgaaaat
     5401 cagcgcgttg ggtttttctt tcaaagtatt aattacgggt tgaacgtctt ttaaagtttt
     5461 gtccaaactt tgcagcgtat tttgtacgtc gccgtagata ggcgattgcg gcgatacgcc
     5521 ttgcagggtt gtgcgcaact ctttcagggt ttggttcagt tcgttcggaa tgttttgcgt
     5581 ctgcggattg ccggccagtt tgtcaatgga gcttagggcg gcattggcag atttgagtgc
     5641 ggacttgagc tcggcaagcg aaccgttcaa ttcggcaacc gtcttatcca aaggcagttt
     5701 gtcgaacttg tccagcaaat ccaccacttt cgcctgcaaa tcgtccaaac cgccgccttg
     5761 tgtagcgatg acggtatcgc ctgcataaac ggtatgcggt cgcagcttgg gcgaagacga
     5821 aggctgatcg ttcaactcaa tcattttgct gccggtcagc aggttgttgc tggagatggt
     5881 ggcggtcagg cctttgttta aagccgtctg aaattgttgt ttccaatgct ctttgctttg
     5941 ttcgtcggca ttgatttcca aacgggaagg ctcaatgcgg atgcgcacgg gaatccagcc
     6001 gttttcaaac aggtgcaggc tgtcgttgcg gtcgaaataa ggaacgtcgg aaaccacgcc
     6061 gacattcagc cctttgtact cgacgggcga accgacggtc aggccgcgca cggattgttt
     6121 gaaaaacgcg gtgtagtaca gcgagcggtc gtcaggcagg ttggcgactt cgctgcggct
     6181 gtcgtaaagc gtgaagctgt cttcgctttt gacgttttta ctgtttttgg ttttcggcga
     6241 atcaaatgaa atcgcgcccg acagcagggc aggcagaggg gcggaattga gtttgatgcc
     6301 gctgcctgtg gtttcgatat tgatgccgct ttccagccaa aaacggctgg cggaatgaat
     6361 cagtttgtcg ttggggcttt ggatgaagat ggtgtaatgc acgctttggt cggacgggtc
     6421 gaaatgcgcg ctttcgactt gcccgaccat aaagttttca tacaaaacag ggctgttgac
     6481 gttgaggatg cggtcgtttt taccaatcaa attcaagcgc agcccgcttt gcccgatggc
     6541 ggtaacgggc ggaatgtcct gcacttggaa cacgtctttt gcctcgtcgc ttttgccggg
     6601 tgtaaaggcg atgtacgaac ccgaaagcag cgtacccaaa ccggttacgc cgctttggtc
     6661 gatacgcggc ttgaccaccc aaaactgggt atcgctgcgg atgaggccgg atacgtccgc
     6721 attgagttgg gcggttactt ccacgccttt ttggtcgtcg cgcagtttga ttcgggtaac
     6781 gcgtccgaca tcgatgctca atactttgat gaccgtattg ttgacctcaa tgccttccgc
     6841 gctgtccatc aagagcgtaa ccacaggccc cctgttgcgg atttccttaa tccacagcca
     6901 gccgccggca atcagcgcga tcagcggaac cagccagacg gcagagagga aggtgttgtt
     6961 tttgcggacg cgtgcttggg cgtgtccgtt tggaggaggg ctgttgtcag tcatgttttt
     7021 ccgtttcatt gaaagcaatg ccgtctgaag cgcgtttgtc ccaaagcagg cgcgggtcga
     7081 aataataggc ggacagcatc gtcagaatca cgaccaggca gaaatagact gccgcactgc
     7141 ccggaatgac gcgcgcggca taagtgtgga acgaacacat caaaataata atcacaaaaa
     7201 tatcaatcat cgaccagcgg ccgaccgctt cggtgatgcg gtagaggtgc gacaatttct
     7261 ttgcgcccgt tggcaaagcg aagcgggcgg acgcaatcaa aaccgacatt gccgcaatct
     7321 tcagtaccgg caccaaaata ctcgcgctga aaataaccgc cgcaatcagc ctgtcgccct
     7381 cgtcccacat ataagcgatg ccgttaagga tggtattgac ctccgtggcg gcaggattgg
     7441 acgaaatcat aatcggcagg atattggcag ggaaatacaa aataaccgcc gccgtcagaa
     7501 acgccgacga aatactcaga cttttcggcc gtcggcggta cagttccgca ccgcacacgc
     7561 cgcaggggga ttcggcactg tcgcggaaat acaggcagcg gctgcaacag gttttacctt
     7621 ccgatgccgt ctgaaccgca ttatcccccg tcagccgccc gatttgaaaa tacacccaat
     7681 gctggggaac cgataccgaa gtccgaatca gcataaccga cagcgcgaac atcagataaa
     7741 acgccgatcc gaagcgaacc tctgccacag acgagagctt gatatacgcc accaaagtgg
     7801 aaacaaaaaa cacatccacc atcatcgcct gtctcaagcg caccatcaca cgcgttgcca
     7861 aacgcagcgc aggatacgcc tgtttccgta tcagcgcggc atagacatac aggcacagca
     7921 gcagaaacag aaccggcgcg ccgaaggtca gcacaaacat cacttcggcc aaaaaaccat
     7981 aatcctgaaa caccatcagg cgcatcatct cgggcagcga aaggacggat gccgcacccg
     8041 gtatcccgac ctcgatatac gtcataccgt aagcaaacgc catcaaaatc agcgaagtcg
     8101 ccgcataagc gggcggggcg gaaaaaggat gcctgcccac cctgaagagc ttatagccgc
     8161 agcgcgggca gaacgccgcc tcccccctgt tcaattgcgg cacatccgcg cggcagccgc
     8221 attccgggca atctaccgta tgcggcggca gggcttcgtc gcggcagttg ccgcgaaggc
     8281 tgtgggggat attttcggca aacggcttca tcgggaaagt ggcaaatata agtggcggca
     8341 ttataaagga aaaaagcaac gcacccaatc aggatgcaaa accggcaggt tcggcagtat
     8401 gaacaaaccc ttggtgtccg gcaaaaatat tttttgagcg gctgtccgcc gactgccgaa
     8461 cccatttgat aaaatcccga ttctgtaaag aatagtaaaa tacctgctta aaaacatcac
     8521 gcaaatgata ttttttccaa aaatatgtga taataaattt cgtttatgat tctttattat
     8581 ttttatagtg gattaac
//
LOCUS       DAXHKX010000076         8594 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHKX010000076 DAXHKX010000000
VERSION     DAXHKX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.076"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            302..775
                     /locus_tag="ACFMWZ_001276"
     CDS             302..775
                     /locus_tag="ACFMWZ_001276"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC5935236.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKK
                     YIDEVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDF
                     FEGKGVDVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(984..2369)
                     /gene="pntB"
                     /locus_tag="ACFMWZ_001277"
     CDS             complement(984..2369)
                     /gene="pntB"
                     /locus_tag="ACFMWZ_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC5935237.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(2435..2818)
                     /locus_tag="ACFMWZ_001278"
     CDS             complement(2435..2818)
                     /locus_tag="ACFMWZ_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC5935238.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2822..4363)
                     /locus_tag="ACFMWZ_001279"
     CDS             complement(2822..4363)
                     /locus_tag="ACFMWZ_001279"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC5935239.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            4653..5486
                     /gene="serB"
                     /locus_tag="ACFMWZ_001280"
     CDS             4653..5486
                     /gene="serB"
                     /locus_tag="ACFMWZ_001280"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC5935240.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKAQAAADACINFGGLERVRGLF
                     G"
     gene            5550..6773
                     /locus_tag="ACFMWZ_001281"
     CDS             5550..6773
                     /locus_tag="ACFMWZ_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC5935241.1"
                     /translation="MGRRKLWFALAAAGVIGGLVSIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVAQASSMRRVAVLTLCGAIAGGGWWLLKRFGKPQIGIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFASAGGKRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     STSLLWFSAVIGPILGVTAVFFQRTAQKFPFIKRDNIKIILLAVCMFALVGAVAVWFP
                     EVLGNGKAGNQLTFGGLTDWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6934..8514
                     /gene="purH"
                     /locus_tag="ACFMWZ_001282"
     CDS             6934..8514
                     /gene="purH"
                     /locus_tag="ACFMWZ_001282"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC5935242.1"
                     /translation="MSVIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLPEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPEIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIATAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKREPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1949 a         2323 c         2406 g         1916 t
ORIGIN      
        1 actcattagt tacctaaaac ttaaaaagca gaaaccttta cgccgtcatt cccacgaaag
       61 tgggaatccg ggaacttaac gttacagtga tttatcggaa acggctgaaa ccgaatgaat
      121 tggattcccg cctgcgcggg aatgacgaat ccatccgtac ggaaacctgc accacgtcat
      181 tcccacgaac ctacattccg tcattcccac gaaagtggga atccaggacg aaaaatctcc
      241 agaaaccgtt ttacccgata agtttccgca ccgacaggtc tggattcccg cctgcgcggg
      301 aatgacgatg caggtatttc tgacgattcc cggctatgat gttgaggcag aaatcgaaaa
      361 attcgtttgg atggatgctg tgatttggca gatgccgagc tggtggatgc acgagccttg
      421 gacagtgaaa aaatacatag acgaagtatt aaccgctgga cacggcaaac tctaccaaag
      481 cgacggcaga cacagcgtca atccgactga gggctacggc acaggcggct tgttgcaagg
      541 caaaaaacat atgctttcac tgacttggaa tgcgccgatt gaggcgttta cccgcgaagg
      601 cgatttcttt gaaggcaaag gcgttgatgt tttgtatatg cacttccaca aagccaacga
      661 gtttttgggt atgacccgcc tgccgacatt cttatgtaac gatgtggtta aaaatccgca
      721 agtggaaaaa tacttggcag attatcaggc acacttggaa aaagtgttcg gctaattaaa
      781 aatccatctt caacacggag atggattttg tttgtttcgt tgattttgtg tcagtttcag
      841 atgtaggtgc ttattcggac gggccgtctg aaaatgtttg ccccaatgca aaaaaaatca
      901 ctgcaaacct tcataaacgg ggtttgcagt gattttttca aatcaaacag attgaaaacc
      961 tgcgccgaat tgttcagacg gcattatttt ttcagttcgg acagaatgtc atccacggtt
     1021 ttcttcgcat ctccgaaaca catcacgctg ttttcgttga agaacagtgg gttttgtaca
     1081 cctgcgtagc cggtattcat cgagcgtttg aagacgacga cttcttttgc cttccacact
     1141 tccaacacgg gcatacccgc aatcgggctg ttcgggtcgg tttgggcggc ggggttgacg
     1201 gtgtcgttcg caccgatgac caagaccaca tcggtttcgg ggaagtcgtc gttgatttcg
     1261 tccatttcca aaacgatgtc gtaggggact ttggcttcgg cgagcagtac gttcatatga
     1321 ccgggcaggc ggccggcgac ggggtggatg ccgaagcgta cttcggtgcc gtttttacgt
     1381 aaaagctcgg tgatttcggc aacggggtat tgcgcttgtg cgactgccat accgtagccc
     1441 ggggtaatga tgacattgtt tgcgcctttc agcatttcgg caatatcggc agctttgact
     1501 tctcggtatt cccctatctc ttggctgccg gaagataatg tgccgctgtc gctgccgaaa
     1561 ccgccggcaa ttaccgagac aaacgagcgg ttcatggctt tgcacataat gtaggacaga
     1621 atcgcgccgc ttgagccgac cagcgcgccg gtaacgatga gcaggtcgtt ggagagcatg
     1681 aagcctgccg ctgcggccgc ccagccggag taggagttga gcatggacac gaccacgggc
     1741 atatctgcgc cgccgatgga ggcaaccaag tgccagccga atgcgagggc aatcagggtc
     1801 ataatcagca ggatgaagcc gctaccgtta atgccgacga atacgagcag caacacaaac
     1861 gatacggcga gcgccagtgc gttgagcttg tgtttggcgg gcagttgcag cgggctgctg
     1921 ctgattttgc cgttgagttt gccgaatgcg accagcgagc cggtaaaggt taccgcgccg
     1981 atgaaaatgc ccaaatagac ttcgaccaga tggatggtgt gcatatcgtg cgaaacgttg
     2041 cccggctcga tatagctgtt gaagccgacc aaaaccgccg ctaggccgac gaagctgtgc
     2101 agcagggcaa tcagttcggg catttcggtc atttccacct ttttggcttt gtagatgccg
     2161 attgccgcgc cgatgagcat ggcgatgatg atccagccca gtccgtgggt attgtcggaa
     2221 aaaacagtta caaaaagggc gaccgccata ccggcgatac cggaatagca gccctgtttg
     2281 gcggtttcct gtttggacag ccccgccagt gagaagatga ataaaattgc ggcaacgata
     2341 tacgccgctg ttacgagtcc tgaagacatg gaaattctcc gattttcgat gatttgtttt
     2401 taatgccgtc tgaactgttc agacggcatc tgtttcaagc agccgcgaca aacagcccga
     2461 cggtgcaggc aaatccgccc gcccacatca gcgagcgcaa tgctgctttg tctgcgatgt
     2521 agcaccagat aaaggcgagg cggaacagga tgaacaggcc ggcaagcgtg ttgacggttg
     2581 cttgtccggc attgccggtt gcgtgtgccg tcaaaacggc ggcggcaaac ggtgcaaagg
     2641 cttcaaaacc gttttgctgc gcggcgtggg cacgggcggc tgtgccttgc gtgcgcgcca
     2701 gaaaatcgcg cggattgtgg ttgtctttaa accggaatcc gcccgctttt ttggcatatg
     2761 ccgcacaaaa aagcggcaat aagcaggcaa tcaggatgca ccaataggca aaagtcatgc
     2821 tttatccttt cctaaacata ttcaacatgc ggcgcgttac gaagaagccg ccgaagatat
     2881 tgatgctggc aatcaggatg gcgatgaagg caagcagggt aacgaaaccg ttgccttggc
     2941 tgatttgcag cagcgcgccg acgacgatga tgccggagat ggcgttggtt accgacatca
     3001 gcggtgtgtg cagcgagtgg ctgacgttcc aaacgacata gtagccgatg acgcaggcaa
     3061 ggacgaacac gataaagtgg ttcaggaatg ctgcgggtgc gaccgcgccg acccacagca
     3121 ccaatacggc ggcgatgacg gcgggcgcga gttttttcca cagggaaaca ggtttcggct
     3181 cgggcttggc ggcaggcgcg gctttttcag acggcgtttg ctgcggctgg gcggaaactt
     3241 gaatcggcgg aggcgggaag gtgatttcgc cgtcgtgggt aacggtcatg ttgcggataa
     3301 tcacgtcttc gaagtccaac gtgatttcgc cgtctttgtt cgggcttaac agcttggtca
     3361 ggttgaccaa gttggtggcg taaagctggg aagactgtcc ggcaaggcgg tttgccatgt
     3421 cggtgtagcc gatgattttc acgccgttgc cggttacgga caattcgccc gggcgggtga
     3481 gttcgcagtt gccgcccgtc gccgccgcca aatcgacgat gacggagccg gatttcatgc
     3541 tttccaccat ttctttggta atcagcttgg gcgcgggttt gcccggaatg gcggcggtgg
     3601 tgatgatgat gtccacttct ttcgcctgct cggcaaagag cttcatctcg gctgcgataa
     3661 attcgtcgct catcactttg gcgtagccgt ctccgctgcc gcccgattct tgtgggaagt
     3721 cgagtttcag gaacttgccg cccatcgatt cgatttgttc cgccacttcc aagcgggtat
     3781 cgaacgcgcg caccactgcg ccgagcgagt ttgccgtacc gatcgccgcc aaacctgcca
     3841 cacctgcgcc aatcaccaaa acctgcgcgg gcggcacttt gccggcggcg gtgatttggc
     3901 cggtgaagaa acggccgaag gcgttggcgg cttcaattac ggcgcggtag ccgctgatgt
     3961 ttgccatcga agacaaagcg tccaaagcct gcgcgcgcga aatgcggggc accatgtcca
     4021 tcgccagcgc gttcactttc ttggcgcgca aggcttcgac caaagcctcg ttttggcgcg
     4081 gccacaggaa gctgacgatg gtttgacctt cgttcaaaag cggcagctcg ccttcggacg
     4141 gcgcattgac cttataaatc aaagggcagg cccaaaccgc cgctttgtcg gcaacggtcg
     4201 cgcctgctgt ttggtaagcg gcatcgtcca aacttgccgc caaacctgca ccgctttcga
     4261 caacggtttc aaagcccagt ttgcccagca gggcgacggt ggcgggcgtg caggcgacgc
     4321 gggtttcgcc ggataatgac tcgcgtggga taccgatttt catctctgaa tcctttttcg
     4381 ggtagtttat atgtatcgtg ggttaaattt aaatcggggc agggcggagc aacgccgtac
     4441 cggtttaaaa ccgattcact tcaaatgtta atatatttta gataatcccc ttataacgaa
     4501 ttttcatcag gctggcaata gttgcggcat tttcccgtgt tgtccgacac atactgccgt
     4561 ttgcgtttga agtcattttc cgattgcagt cccgcccgcc gtaacattct ttgccgtttc
     4621 actatataat ccgcattttt tgagttaccg ccatgccgca cgccctcgtc ctccaatttc
     4681 cctccgccga agccctgcct tccgacttcc cctcacgcct gcccgaacct gattacgccg
     4741 atgaaaagcg tatgcgtttt atcgttgaag aagggttttc tttaagcgag gcggacgcgg
     4801 cgttgcttgg cagccgtcaa atcgaccacg ccgtgttgcc ggatatggat ttcgacgaac
     4861 tcggtttaat tgtcagcgat atggattcga cgctgattac cattgaatgc gtcgatgaaa
     4921 ttgcggcagg cgtgggttta aaagacaaag tagcggaaat taccgagcgt tcgatgcgcg
     4981 gcgaactcga tttcgaacag tctttacgca gccgcgttgc gctgttggcg ggattggacg
     5041 aacagatttt ggcggacgtt tatgaaaacg ttttgaagct ctcgcccggt gcggaattct
     5101 tgttggacga atgcaaaagg cacgatgtga aattcctgct ggtgtccggc gggttcacat
     5161 tttttaccga aaggctgcaa caacgactcg gcttcgaata ccaacacgcc aatgttttgg
     5221 aaattgaaaa cggcaggttg accggtcgtc tgaaaggcag aatcatcgac gcgcaggcaa
     5281 aggcggattt attgcgcgaa taccgcagcc gcctcggatt gcagccgcat caggtgttgg
     5341 cgatgggcga cggtgcgaac gatattccga tgctcaaaga agcgggcata ggcgtggctt
     5401 accgtgccaa accgaaagcg caggcagccg ccgatgcctg tatcaacttc ggcggtttgg
     5461 agcgtgtacg cggcctgttc ggataggcgg ataggaaacg gatgccgtct gaaaggtttt
     5521 cagacggcat ttgaacggca ggaacgacag tgggacgcag aaaactttgg tttgccctgg
     5581 cagcagcggg cgttatcggc ggtttggtca gcattgtgct gacggaactg atgcacttca
     5641 tacaacatac agcatacggt tatggcgcgg acggtgcgta catatcgttc cgcgaaggcg
     5701 tggcacaggc ttccagtatg cggcgcgttg ccgtgctgac gctatgcggc gcgatcgcag
     5761 gcggcggctg gtggttgctg aaacgtttcg gcaagccgca aatcggaatc aaggcggcat
     5821 tgaagcagcc gttgcagggg ctgccgtttc tgacgacggt ttttcatgtt ctgctgcaaa
     5881 tcataacggt cggactcggt tcaccgctcg gacgggaagt cgcgccgcgc gaaatgactg
     5941 ccgcgtttgc ttcggcaggc ggcaaacgct tgggtttgga cgaaggcgaa atgcggctgc
     6001 tgattgcttg cgcttcgggt gcgggtttgg cggccgtgta taacgtgccg ctcgcctcca
     6061 cgctgttcat cctcgaagcc atgctgggcg tgtggacgca gcaagccgtc gccgccgcat
     6121 tgctgacttc cgtcatcgcc accgccgtcg cgcgcatcgg cttaggcgac gtgcagcaat
     6181 atcatcccgc caaccttgcc gtcagcactt cattactttg gttttccgcc gtcatcggtc
     6241 cgatactggg cgtaaccgcc gtctttttcc aacgtaccgc ccaaaaattc ccctttatca
     6301 agcgcgacaa tatcaaaatc attctcttgg ccgtctgtat gtttgcactc gtcggcgcgg
     6361 ttgccgtttg gtttcccgaa gttttgggca acggcaaagc aggcaaccaa ctgacctttg
     6421 gcggcttgac cgactggcaa cacagccttg agctgaccgc cgtcaaatgg ctggtcgtat
     6481 tgatggcact tgccgccggt gcatacggcg gcctgattac gccgtcgatg atgctcggca
     6541 gcaccatttc ttttgctgcc gccgccgcgt ggaacagcgt cttccccgaa atgccgtctg
     6601 aaagcgcggc agtcgtcgga gcggcggtgt ttctcggcgt atcgctcaaa atgccgctga
     6661 ccgccattgt gttcgtattg gaactgacct acgcgccgct gtccctgctg ctgcctttgt
     6721 gcatcggcat ggcgggggca ttgggaacat cgggaaaaat gggcttcaaa tgaatgccga
     6781 caaatgccgt ctgaaaggtt ttcagacggc atttctttgt aaattgtcaa caatatctgc
     6841 aaaaacttac tgccaaaaaa ctgaaaatag cggataatac cgccccgaaa aagttcccct
     6901 ttccccatcc aactgtcaga aaaggaagcc ccaatgtccg tcatcaaacg cgccctgatc
     6961 agcctgtccg acaagacggg cgcagtcgaa tttgcccaaa ccctgcacaa gctcggtgtc
     7021 gaaatcctct ctaccggcgg cacggcgaag ctgcttgccg atgcgggcgt tcccgtgatt
     7081 gaagttgccg actacaccgg tttccccgaa atgctcgacg gccgcgtgaa aacgctgcat
     7141 ccgaaaatcc acggcggtat tttgggccgg cgcgatttgc ctgaacacgt cgccaagatg
     7201 gaagaacacg gcatcggcaa catcgacctc gtgtgcgtca acctctaccc cttcgctgcc
     7261 accatcgcca aaccaaactg cacgctggaa gacgcgattg aaaacatcga catcggcggc
     7321 ccgaccatgg tgcgctctgc cgcgaaaaac tggaaacacg tcgccatcgt tactgacaca
     7381 gccgatttcc cggccatagc tgccgaactc gaagccaaca acggcgcatt gagcgacaaa
     7441 acccgtttca acctgtcgcg caaagcgttc agccacaccg cccaatacga cggcatgatt
     7501 tccaactacc tgacctcgct ttcagacgac gttttgagcg gagagcctga aatcggcgaa
     7561 tttccaagtc ggttcaacca aagctggatt aaagtgcaag acatgcgcta cggcgaaaac
     7621 ccgcatcagc gcgccgcgtt ctaccgcgat attgaccccg ccgcaggcag cctttccgcc
     7681 tacaaacaac tgcaaggcaa agaattgtcg tacaacaaca tcgccgatgc cgatgccgca
     7741 tgggaagccg tcaaatcctt tgacgcgcca gcctgtgtga ttgtgaaaca cgccaatccg
     7801 tgcggcgtag ctgttgcagc cgataccttg accgcctaca aactcgccta cgccaccgac
     7861 accaccagcg cgttcggcgg catcatcgct ttcaaccgcg aagtggacgg cgaaaccgtc
     7921 aaacaaatta ccgacaacca gtttatggaa gtcctgatgg cgccgaagtt taccgccgaa
     7981 gccctcgaaa ttgccaccgc caagaaaaac gtgcgcgtgt tggaagtgcc tttggaagca
     8041 ggcgcgaacc gcttcgaact caaacgcgtc ggcggcggac tgttggtaca aacccccgac
     8101 attcaccgcc tcaaccgcgc cgatttgaaa gtcgtctcca aacgcgaacc gaccgagcaa
     8161 gaatggaacg acctgatgtt tgtttggaac gtcgcaaaat acgtcaaatc caacgccatc
     8221 gtcttcggca aaggcggtca aacctacggc atcggcgcag gccaaatgag ccgcgtggac
     8281 agtacccgca tcgccgcccg caaagcgcag gacgcaggct tggacttaaa cggcgcatgt
     8341 gccgcctctg atgctttctt ccccttccgc gacggcgtgg acgtgattgc cgagcagggc
     8401 atcaaagcca tcatccatcc ggcaggttcg atgcgcgatc aggaagtctt cgacgcagcg
     8461 gacgaacacg gcatcgccat ggtcgtaacc ggcatccgtc atttccgcca ttaatgcaga
     8521 taaacaaggt aatgccgtct gaagggcttt cagacggcat tttgcgctat tttgtgaagg
     8581 tagggatgac ggtt
//
LOCUS       DAXHKX010000077         8542 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHKX010000077 DAXHKX010000000
VERSION     DAXHKX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.077"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(83..1129)
                     /gene="recA"
                     /locus_tag="ACFMWZ_001283"
     CDS             complement(83..1129)
                     /gene="recA"
                     /locus_tag="ACFMWZ_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC5935243.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGKLDETDGERPEE"
     gene            1383..2147
                     /gene="aroD"
                     /locus_tag="ACFMWZ_001284"
     CDS             1383..2147
                     /gene="aroD"
                     /locus_tag="ACFMWZ_001284"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC5935244.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2167..4182
                     /gene="rep"
                     /locus_tag="ACFMWZ_001285"
     CDS             2167..4182
                     /gene="rep"
                     /locus_tag="ACFMWZ_001285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC5935245.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGDPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINNLLKEIDYENHLLASEESKAGEIKWRNVGDL
                     VSWFARKGGEDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4250..5308)
                     /gene="dinB"
                     /locus_tag="ACFMWZ_001286"
     CDS             complement(4250..5308)
                     /gene="dinB"
                     /locus_tag="ACFMWZ_001286"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC5935246.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQGLWA"
     gene            complement(5452..8540)
                     /locus_tag="ACFMWZ_001287"
                     /pseudo
     CDS             complement(5452..8540)
                     /locus_tag="ACFMWZ_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
BASE COUNT         1963 a         2337 c         2238 g         2004 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggctgct caaaaaaaac ggtacttcgc aacagaagta
       61 ccgtttatcg ggattttaag ttttattctt ctgggcgttc gccgtcggtt tcgtccaatt
      121 ttccttcggt gatgtgcatt tctacgccgt tgagggcgcg aatttttgcg tcgatttcgt
      181 cggcaatttc gggattttcc ttcagccaga cgcggacgtt gtctttgccc tgaccgattt
      241 tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt ttcacgccga
      301 tgtcgatcaa ttcgccttcc caactgatgc cttctccgta gaggatgtca aactctgcct
      361 gacggaacgg gggggcgact ttgtttttga tgactttgac gcgggtttcg ttgcccaata
      421 cctcttcgcc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg gaagaataga
      481 atttcagcgc gttgccgccg gtggtggttt cggggctgcc gaacattacg ccgatcttca
      541 tccggatttg gttgatgaac acaaccagcg tgttggtttt tttgatgtgt ccggtcagtt
      601 tgcgcaaagc ctggctcatc aggcgcgcct gcagtccgac atggctgtcc cccatatcgc
      661 cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc atatctatgc
      721 cgcccgaacg gacgagtgtg tcgcagattt ccaaagcctg ttcgccggta tcgggctggg
      781 acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga tcgaaggcgt
      841 gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg acggcttcga
      901 ggcagagggt ggttttgccg gaggattctg ggccgaagat ttcgacgatg cgcccgcgcg
      961 gcagaccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa atgacttcga
     1021 ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg aaactttttt
     1081 cgatttgcgc cagtgcggcg gcaagtgctt tgcttttgtc gtctgacatt ggggttactc
     1141 cggaacaaat gcggtatgtg ggatgcggcg caacacgggc tgcggcgcgg gatgtgtatc
     1201 gttttcccga tgtgcgggct atcggtaatg ctgcttcacg aggttgccat tatcgcatat
     1261 ttccttgctt gccgatatgc gccaggacgc ggcggcttgt gccggaatgg aatctggatg
     1321 ccgtctaaaa ggcggccggc tttgttataa tggcggctgt tttttctgtg tgtgcctgtt
     1381 ttatgtgttc ctgccttgtt gtcaaaaata ccgttatcgg aagcggacgc accaaaatcg
     1441 ccgtgccgct tgtcgcccgc gatgccgccg aactttccgc cgtacttgag caaatcaaaa
     1501 atatgccctt cgatattgcg gagttccgcg ccgacttttt ggaatgcgcg ggcagtatcg
     1561 gcgaaatatt gcaccacacg cagaccgtcc gcgacgcgct gcccgacaag ccgctgctgt
     1621 ttacgttcag acggcatggc gaaggcggct cgttcccgtg ttcggacgat tattatttta
     1681 aactgctcga cgcgctgatc gaaagtcgcc tgcccgacat tatcgatatc gagctgtttt
     1741 ccggcgaaac cgccgtccgg cgcgctgtgg cgaatgctca aaaaaacggc atcgccgccc
     1801 tgctctgcaa tcatgagttt caccgcacgc cgccgcaaga agaaatcgta tgccgtctga
     1861 aacagatgga ggactgcggc gcggacatct gcaaaattgc ggtgatgccg caaagcgcgg
     1921 aagatgtgct gactttgctt tccgccacgc tcaaagcgaa agagcttgcc gccaaaccga
     1981 ttgttacgat gtcgatgggg cagacggggg cggtcagccg gcttgccgga caggtgttcg
     2041 gctcaagcat cacgttcggt tcgggaacgc aaaactccgc gccggggcaa atcggcgtat
     2101 ccgccctccg tgcgacactc gactgcctcg aaaacggcgc agactgattt cagacagcat
     2161 caaaacatga tgaaactcaa tccccaacag ctcgaagccg tccgctacct cggcggccca
     2221 ctgctcgtcc ttgccggtgc aggcagcggc aaaaccggcg tgattactca aaaaatcaag
     2281 tatttgattg tcaatgtcgg ctatctgccg cataccgttg ccgcgattac ctttaccaat
     2341 aaagccgccg cggaaatgca ggagcgcgtc gccaaaatgc tgcacaaatc gcaaacgcgc
     2401 gggctgacga tttgcacgtt ccactctttg ggcatgaaga ttctgcgcga agaggcgaac
     2461 catatcggtt acaaaaaaaa cttctccatt ctcgattcta ccgacagcgc gaaaatcatc
     2521 ggcgaactct taggcggtac gggcaaagaa gccgtattca aggcgcaaca tcaaatctcc
     2581 ctgtggaaaa acgatctgaa aacgcctgaa gatgtcgttc agacggcctc taatgcgtgg
     2641 gaacaacaaa ccgcacgcgt gtatgcgagc tatcaggaaa ccttacaaag ctatcaggca
     2701 gtggacttcg acgacttaat ccgcctgcct gccgtgctgt tgcagcaaaa cagcgaggtg
     2761 cgcaacaaat ggcagcggcg gctgcgttat ctgctggttg acgaatgcca agatacgaat
     2821 acctgccaat ttacgttgat gaaacttctg accggcgcgg aaggtatgtt taccgccgtc
     2881 ggcgacgacg accagtccat ctacgcatgg cgcggcgcaa acatggaaaa tctgcgcaaa
     2941 atgcaggaaa actatccaca gatgaaggtc atcaaactgg agcaaaacta ccgctccacc
     3001 gcgcggattc tcaaaatcgc caacaaagtc atcgaaaaca accccaagct gtttaccaaa
     3061 aaactttggt cgcaattggg cgaaggcgat cccgtcaaag tcgttgcctg ccaaaacgag
     3121 cagcacgaag ccgactgggt cgtcagccaa atcgtcaaac aaaaactcat cggcggcgac
     3181 aaaacccaat acgccgattt cgccgtgtta taccgcggaa agcatcaggc gaggattttc
     3241 gaggaagcat tgcgcggcgc gcgcatcccc taccgtctct ccggcggaca aagctttttc
     3301 gataaagccg aaatcaaaga cgtgttgtct tatgtgcggc tgcttgccaa ccccaacgac
     3361 gatcccgcct ttctgcgtgc cgttaccacg cccaaacgcg gcatcggcga tgtcacgctg
     3421 ggcaagctca acacttacgc gcacgaacac gaatgcagcc tgtatgaagc cgcacaaaac
     3481 gaagaagccc ttgccacgct gaacaatacc aaccgccaac acctgcaagc ctttatggat
     3541 atgttcggca gctaccgcgc caaagcggaa accggcgaag caggcgagtt catcaacaac
     3601 ctgcttaaag aaatcgacta cgaaaaccat ctgcttgcca gtgaagaaag caaagcaggc
     3661 gaaatcaaat ggcgcaacgt cggcgatttg gtatcatggt ttgcgcgaaa aggcggggaa
     3721 gacggcaaaa acgtcatcga actcgcccaa accgtcgcct tgatgacgct tttggaagga
     3781 aaagacgaag aagaaaccga tgctgtctcg ctatccacgc tacacgccgc caaaggtttg
     3841 gagtatccgt atgttttcct tgtcggttgc gaagaaggtg ttttgccgca caacgacagc
     3901 atcgaagagg gcaacgtcga agaagaacgc cgcctgatgt acgtcggcat cacccgcgcc
     3961 aaacgccagc ttacgctgac ccactgcgtc aaacgcaaaa aacaaggcat atggcagttc
     4021 cccgaaccca gccgattcat agacgaaatg ccgcaggaag atttgaaaat cctggggcgc
     4081 aaaggcggcg aaccgattgt cagcaaggaa gaaggcaggc gcaaccttgc cgatataatc
     4141 ggaaggctcg acaacctaaa aaaaagcggc gcggcggatt aaaccggagc cgcaatgccg
     4201 tctgaaggct tcagacggca tattttttgg acggcgcgcg taaatcggtt tacgcccaca
     4261 agccctgctg ctggtttttc ggcacaagat gtcccacgcc gatgccgata aggcggaacg
     4321 cgtcttccgt ctgcggcggg acacgcgcca tcaacatttg cgcagcctgc agcagagctg
     4381 cgcagtcggg caatacggag gaataagtca gcgtgcgcgt gatgatgcgg aaatcgtagg
     4441 tcttcagctt gagcgttacg ctttgggctt cgacgttttt gcgcgtgatt tgccgccaca
     4501 ggtcttcggc aagatggggc aggtgtccgg cagcctgctc gagcggcagg tcttcgggca
     4561 gggtaatttc tgtggagatt tggaggcgtt cgcgttcggc tttgacgggg cgttcgtccg
     4621 taccgcgcac caaatcatag aggcggtatc cgtagcgtcc gaaatggttt aagagttcgc
     4681 cgcgctcgaa acggcgcaag tcgcccgccg tccgcatacc cagcgactgc atttttttca
     4741 gcgttacctt gcccacgccg gggattttgc ccaaaggcag ggtttccaaa aatgccatga
     4801 ctttgtgcgg cggcaacaca aactgcccgt tcggcttgcg ccagtccgac gcgattttcg
     4861 ccagaaattt gttcggcgcg atgcctgcgg atgcagtcaa acctgtttcc gcaaaaatgg
     4921 cggcacggat ttctttggca acttcactgg cgtaagggat gtttttgaaa ttacgggtaa
     4981 cgtcaagata ggcttcgtcc agcgacaagg gttcgattaa atcggtataa cgcctgaata
     5041 cggcgtgaat ctgcgcggaa acctgacggt acaaatcgaa atgcggcggc acatacaccg
     5101 cttgcggaca cagccttttc gccgttgcca ccgacatcgc ggaatgcagc ccgaactgcc
     5161 gtgcctcata cgatgcggcg caaatcaccg aacgcgcgcc ctcccacgcg acgaccaccg
     5221 gccgcccttt caaatgcggc tgttcgcgca gttctaccga tgcgtagaac gcgtccatgt
     5281 cgatgtggat aattttgcgt gaagacatcg gctcttctga ggataaaagg gatattctac
     5341 tgccggcatc gggcaaattc caaatatacg ccccgatagg cctgcctcca taaaaatgcc
     5401 gtctgaaaca taccctgttt cagacggcat ccgcaaaact acggttttca attaaaactg
     5461 ccaatccagt ttcatgctga cagtgcgcgg ttctccatag aagttgtttg cgccgcgcgt
     5521 acggttgtag ttgttctcaa aataagtgcg tccgtttaag ttcgtaccga tgaggctcaa
     5581 tttggcgtgt ttgcccaatt cgtaacggac gaaaccgtct atcagcccgt agccgccctg
     5641 cctgatgtta tacaggctgc ttgtgccgct ttgtgcggac acgccgccgc cgacggtcag
     5701 ccccgtattc ggtatatgga agctcgttcc aaaacggaat atgtgcacgg gtgtgaaatt
     5761 gctgaagttg tacgggtctg cgcctgtgtt tttggcgagg cgttcggcgt tgacttcggc
     5821 ggcgtttttg tagcggctct tgttgtaggt gtaacccgca aagactttcc aatcttcgtt
     5881 caactcaccc gacaactcgg cctccgcccc acggctgacc actttgccta tcggtttggc
     5941 aacggtttgg aacgacccct gcttgccgcc tgctccggga acatagccaa aatcgacgac
     6001 cgtgcggttt ttctgttcga ggtaaaacaa tgcgaacgaa gcattcagcc gtccttgcaa
     6061 gaatgcgcct ttccagccta cctcatagtt tgtgccgacc aaaggcggca aaacggtttt
     6121 ggcactgaca tcgacattat cctgctgttt gaagattttg gtataacttc cgtaaatact
     6181 ctgttgcggt gtcaagtcat aggtgatgcc tgcatagggc gtcaatttat gaccttgcat
     6241 cttggccgtg taatggtcct gatccgcctt aatactcgat gctgtctgaa aatcgcttgc
     6301 cggctgccca tagcggacag gcatatcttt ggtttgcgag gtttcatagc gcgtgtagtg
     6361 cagcccgccc aaaaggtgca gtcggccggt tacgttgaaa cgcgtgctgg cagtcagcga
     6421 atgggttttg ttggtgttga ggtatttggc gtagttatac agcgcaggaa catggtcatc
     6481 ggccacttta acagttttcc aaaccggcac cgtaccggaa aaaccggtaa agacaggcga
     6541 gccgtcggga ttggtctcct gaatcttgtt gcctttttcg tccagctcat atacatcgac
     6601 atataccggt gtccggctgc cgctgtattc gtcatagtaa tacacctgct tgccttcggc
     6661 atcgagcttg ggctcggttt ttattttctt ggcattcctg cattcttcgg cataaacggt
     6721 acggttgcct ttttcatcgt acgcctgcca atcgggttct ttatgccccc tgaccaaagg
     6781 agaagacaaa tcaccgtccg gctcccctga caacttcccg catacacgcc gtgcgttgcc
     6841 cctgtattcg gacgtactct gtggcggcgt tcgtagattt ccagatattc ggaacgtatc
     6901 ttttcatcgc cgtaggcata gccgacaaag aaatcatgct cccgcccgaa cagcccatat
     6961 gtgccggtca ggtcaagttt aattccccat tggcggtcgt ctttggtatg ccgcaacggc
     7021 atataactgt atcgtcggtt ggcggtagcc ttccgattaa aggaagagtc atacaggctg
     7081 tttggaaaac gttgtgccgc attattaaag atgccttcct tcgcaagggc tttatcgaca
     7141 aattccgact tgtcggcatc aacacccgga tccccccaag aaccttgaca gataaagtcc
     7201 agcgcaaaag gggcactcat acactcgtca aaaccggctt tgcgttctgc ggcacggcgg
     7261 ctgcggtact gctcgaaagc agtattatcg aaacggtttt taacaaaatc gtctttgcgc
     7321 tcacggtatt ccttggcggt ttcgtcacga tatgctttca gtttctccaa tgccttatct
     7381 ttcggctcga acgggatgac ttcgtttttt tcggtcaaaa agcctaccgc atcctcatct
     7441 gacaaaaccg ccgcatgttc gtttttcaga aaaaactgcc ccaccttcgc atcggattca
     7501 ttcttggtat aagacacttc ggcattgagc tgccaaccgt tgtcaaacac atgtttgaaa
     7561 cctgagaaaa ggttgtattt gtcagcactc aaccgcgacc aatcctcccc caaataagtg
     7621 ttgcgcggca gttgcaaagg ccggttgcag gcaggcgttg aactgaacgg ggcagttttc
     7681 tgattttcac aggacaaaat aatgcccgaa aaatcaggaa cctccctact cttctgatac
     7741 atgccgccca aagtaagcac actgctgtcg cccgcatcgg cttcggcaat gccgtaaacc
     7801 atatgtttcc tgccccaaac gcggtcttta aacgattttt tatactcttc cgcacccacc
     7861 aaccttccgc gtaaggtatt cgccttattc aggctgcctg aaacatccaa cactgcacgc
     7921 cggctgccgc gatggtcggc ggtcagctct ccggtatgtt tgaaagaagc ggtaggtcgc
     7981 ttacggatca aattgacggt tccacccggc tctgaattgg attgggtcaa ccccgttgca
     8041 ccccgtacaa cttcaatatg gtcataaacc gccaaatcgg tactcggaga cacgtcgatt
     8101 ttcgccgtat atcccgaacg gcctgcaaca ttgacggtaa taccgtcttc accaatctga
     8161 tcaatataga aaccgcgtga caaaaaccgc gtctgcaagc ctgaatcgcg cacaacgttg
     8221 acacccgtcg tgtttttcat tgcctcttca agcgtatgca ccgccttatc gtcaaggcgg
     8281 ctgcgcgtga tgacgctgac cgactgcggc gtatccttgc ccgcaatcct catacctgtg
     8341 gcggtggaca tccgatctat cgtataagaa cgggtcttct cggtcttgcc cgacagagca
     8401 tgagagccgc gtacattgac cgtatccaga ctgacggtat tgccgtctga aacaggcaca
     8461 acaccgtctg caaaagaacc accgtaagcc gataacagca taacggtcag aattttaagt
     8521 gaaaaatgat tttgattcat ag
//
LOCUS       DAXHKX010000078         8320 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHKX010000078 DAXHKX010000000
VERSION     DAXHKX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.078"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            242..466
                     /locus_tag="ACFMWZ_001288"
     CDS             242..466
                     /locus_tag="ACFMWZ_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC5935247.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
     gene            482..886
                     /locus_tag="ACFMWZ_001289"
     CDS             482..886
                     /locus_tag="ACFMWZ_001289"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFC5935248.1"
                     /translation="MDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIKQ
                     RIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITGS
                     TVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            929..1132
                     /locus_tag="ACFMWZ_001290"
     CDS             929..1132
                     /locus_tag="ACFMWZ_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFC5935249.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            1308..2470
                     /locus_tag="ACFMWZ_001291"
                     /pseudo
     CDS             1308..2470
                     /locus_tag="ACFMWZ_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            2477..2683
                     /locus_tag="ACFMWZ_001292"
     CDS             2477..2683
                     /locus_tag="ACFMWZ_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935250.1"
                     /translation="MFTFLNKNIFIKTIVLGCFYSFLFFSFFVSGKSFGYSFEIFNMD
                     TSALAFLSVFMGFFISVAVHIYDI"
     gene            2707..3042
                     /locus_tag="ACFMWZ_001293"
     CDS             2707..3042
                     /locus_tag="ACFMWZ_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC5935251.1"
                     /translation="MKELTINDLTLVSGGFEFGRDYIEIAPVVGTAGGVASSVRAASM
                     TGEAGAILTRSGVTGLRAGVVAIPLLAYNGTVWVNRNTKLQDNLGRLIDKVTGLDQSG
                     NDYGDGTSY"
     gene            3077..5173
                     /locus_tag="ACFMWZ_001294"
     CDS             3077..5173
                     /locus_tag="ACFMWZ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC5935252.1"
                     /translation="MSIYLSRLSFGFSRRLLVVLQTEAAECGLACLVSVLGFHGFYTD
                     LRHLRARFSLSLKGATLADLVRFANSMNLTARAVRLDLDELANLRVPCILHWDLNHFV
                     VLHEVHRNHIMIMNPASGWQKVRMEEVSRCFTGVALELFPNTQFEEKHETRKIRVLPM
                     LRGVVGLKRSLFQLLLLAAVLQVFALASPFFMQWVIDHAIVSADRDLLLTLALCFGLL
                     MIVRQLVSLLQTWAGMYLSTSLNIQWKANVLRRLMDLPVSYFAKRHLGDVVSRFGSVD
                     SIQSTLTSTFFVLVLNSIMAVFTLGLMYLYSPSLTTVVLVVLLIYIGIRWVAYYPLRH
                     ATQENIVHAAKQSSYFMETIRGIQTVKLFDKNAQRHAAWLNLFIDTINTGLMTQKLSA
                     LFGFTNGLLFGIANILIVYFGAVSVLDGVFTVGALMAFMSYKSQFESKAGSLIDQFVQ
                     IKMLGLHAERLADIVLEETENEQNNDFRLPENLEHFDIQIENVSFRYAENEPYILQDF
                     SLIIKQGTAIAFAGHSGCGKSTLIQILTGSLKPESGHVLLGGHDIHALPPAFIRTWSA
                     SVMQNDVLFTGSIAENISFFDDTPNMEKIAFCAQMANIHHEIVAMPMAYETLIGDMGS
                     ALSGGQKQRIVLARALYREPKILFLDEASSHLDINNERVINDNLRQLNITKIMVAHRQ
                     ETLNTADSVVYLDKVA"
     gene            5175..6437
                     /locus_tag="ACFMWZ_001295"
     CDS             5175..6437
                     /locus_tag="ACFMWZ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC5935253.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(6707..7546)
                     /locus_tag="ACFMWZ_001296"
     CDS             complement(6707..7546)
                     /locus_tag="ACFMWZ_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935254.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKEQERQARRMERDDGHG
                     IGM"
     gene            7587..>8320
                     /locus_tag="ACFMWZ_001297"
     CDS             7587..>8320
                     /locus_tag="ACFMWZ_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC5935255.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAILPGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLELINRQLTEKGLKIEKASAAVIDATIIQTAGSKQRQAIE
                     VDEEGKVSGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECK
                     HLSPLLEGIAESTTVYADKGYDSAENR"
BASE COUNT         2503 a         1577 c         1703 g         2537 t
ORIGIN      
        1 gggtttcggc tgttggggga agggcttttt tgcaaaggtc tcaatttaaa ctttaacgca
       61 atatcaactc attttctgcc acatatctca aaaaagtcta gtttgataca gtaatctctt
      121 taacagcttt tcttgctaaa atttaaaaca aaatgtaaat attcttttat ttatttaaat
      181 aatagattaa aatattattt aattaaaagc ataatgagcg tagatattca aaggagctaa
      241 catgaaagtt atttccgtat taaatcaaaa gggtggaagt ggtaaaacca cagtcgcaac
      301 tcacttggca cgcgccttgc aattagcagg aaatagcatc ttattggttg atagcgatcc
      361 acaaggaagc agtagggact gggctgctgt tcacgaagag caacctgttc ctgtgattgg
      421 tattgacagc ccgacaattg atcgtgattt aaaaagcgtt gtttaaaaag attttgttat
      481 tattgatggc gcaccacaaa cagccgattt agccatatca gcgattaaag cctctgattt
      541 agttttaatc cctgttcaac cgtcgccata cgatatttgg gcaaccgctg atttggttaa
      601 tttaatcaag cagcggataa aactcataga tggaaattta aaggctgctt ttttaattaa
      661 ccgaactatt aaaggaacaa agattggcgg agaaattaga gcagcgttag aagaatatga
      721 gttacctgtt ttatcaaccg tgattgtgca acgtattatt tatccaacat ctgctatcac
      781 aggtagcact gtactggata aagaaccaaa tggagaagca gctaaggaaa ttcaatcact
      841 tgcgaatgag ataataaatt tgataaatat agataatcat atttaatgaa atattatttt
      901 aaatttttaa atattgtagg aaataattat gaacggatta aaagcagatc gtcctagtgc
      961 aaacaaagca aacttatcta ttgatgatgt gatcaagcaa gaagaaatca agagaatcag
     1021 ttttgatatg tctgaatctc tgcatagaga attgaagaaa tacgcggctg agaatggtgt
     1081 aactattcgc gtcgtgctca ataaactcgt ttcagattta ttgaataaat aataacacac
     1141 ggcagaatgt atttgaccat aatgtcaaag caatatccaa ctttatctaa aaataacaaa
     1201 ataccttctt ccaaaaacag aacgtctttt cattgcaaaa cttgacatat aaacactagt
     1261 taagtaaact taattttcat tattaataat gaaaataagg attatttttg catattaact
     1321 tttcaatgga atataaagaa tttaatgaaa attatttata taaaaagcca tttattttta
     1381 aaaatgcttt agatgttagt tctatctcat ggaaagaaat taatgagttg tatcaacgtg
     1441 cagaccctac tgattggcaa tttaaatttc gcaaagggga aataatcccc aaagaagcct
     1501 atgttgaatc attcaatgat gtgggtagaa tacgtcatcg gtttaataaa acagccgtat
     1561 atcagtatct gcaagatggt gcaacaatgg tttataaccg tattgataat gagccatttg
     1621 ttgacagtat tgctaaacaa attgcccagt ttgctcaagc acaaactgtt gttagtggtt
     1681 atttagcgtt tggtagctca tcatcatatc gtaatcattg ggatactcga gatgtatttg
     1741 cagtacaatt gattggcaaa aaacattgga caatttctgc cccaaatttt gatatgccgt
     1801 tgtatatgca gcaagcaaaa gatatgccgc acattactcc atctaaaaca gtggatatgg
     1861 aagtcatttt agaagcagga gatattctat atattccacg tggttggtgg cataatccta
     1921 tgcctatgaa ttgtgaaaca ttccatttag ctattggaac ttttccgcca aatggttata
     1981 attatatgga atggttgatg aaaaaatacc tgatatccag agtattcgtc aaaatttcat
     2041 agactgggaa cacgaccaaa aaaatataga taatgcggct caggcagtta ctgaaatgat
     2101 gaagaaccaa gaaaattatc aagcattcat acaagatttt ttgggaaatc aacgcgtaaa
     2161 tactgcattt aatatgcaaa tttttggaaa tctggataat gacagattac ctgaaaatag
     2221 tacaattaaa ctcaattcgt tggataaccg tacaattaaa caaggatata tcatagcgaa
     2281 tggcatcaaa accaacttgg acaatgatag ccaaacgatt ttgcaatgga ttgctgacaa
     2341 acatagtgtt aagttaactc aactgtatga attttgccaa aatcaaaaca ttaatttgga
     2401 aaaagtggaa aaattagtgt ttgatttgac aatgatcgat gtattggaat gtttaactga
     2461 tgaaagataa attattatgt ttactttttt aaataaaaat atatttatca aaacaattgt
     2521 acttggctgt ttttattcct ttttattttt tagttttttt gtatctggta agtcatttgg
     2581 ttattcattt gaaattttta atatggatac aagtgcgctt gcttttttaa gtgtatttat
     2641 gggctttttt ataagcgtgg ctgtgcatat ttacgatatt taatacaatt gaaaagataa
     2701 ataattatga aagaattaac tattaatgat ttaaccctag tttcgggtgg atttgaattt
     2761 ggtcgtgatt atattgaaat agctccagtt gtaggtacag caggtggcgt tgcctcttct
     2821 gtgagagcag cttcaatgac tggagaagct ggagcaattc ttactcgttc aggagtaaca
     2881 ggattacgtg ctggagtggt agctattcca ttgttagcct acaatggtac tgtatgggtt
     2941 aatagaaata ccaaattaca agataattta ggtagattaa tagataaagt aactggcctg
     3001 gatcaatcag gcaatgatta tggcgatggg acaagttatt aatctcataa tactttaaaa
     3061 tcaaaaaaga acaccaatgt caatatatct atctcgccta tcattcggct tttctcgccg
     3121 tttactagtt gtcttgcaaa cggaagcagc agaatgtgga ttagcgtgtt tggtatcggt
     3181 gttggggttt catggttttt atacggattt gcgccattta agggcgcgtt tttctttgtc
     3241 gcttaaaggc gcaacattgg cggatttggt acggtttgct aatagcatga atttaaccgc
     3301 tcgggctgtt cggttggatt tagatgagtt agcaaatttg cgcgtaccct gtattttgca
     3361 ttgggattta aatcattttg tggtgttgca tgaagttcat cgcaatcaca ttatgattat
     3421 gaaccctgct tcgggttggc aaaaagtgcg tatggaagaa gtctcgcgct gttttacagg
     3481 tgtagcgttg gaactatttc ctaacacgca atttgaagag aaacacgaaa cgcgtaaaat
     3541 tcgcgtacta ccgatgttgc gcggagtggt gggattaaaa aggtctttat ttcagcttct
     3601 gctgctggcg gcagtattac aggtgtttgc tttggcaagc ccttttttta tgcaatgggt
     3661 aatagaccat gccattgtat cggcagaccg tgatttattg ctgacattgg cattgtgttt
     3721 tggcttgttg atgattgtgc ggcagttagt gtcattgttg caaacttggg cgggtatgta
     3781 cttatctacc tctttgaata ttcaatggaa agctaatgta ttacgccgct taatggattt
     3841 gcctgtttct tatttcgcta aacgacattt gggcgatgtg gtttcacgtt ttggctcagt
     3901 ggacagtatt caaagtacgc tcacttctac tttctttgtg ttggtgctca acagcattat
     3961 ggcggtattt acattagggt tgatgtattt gtatagccca agtttaacga cagttgtact
     4021 ggttgttttg ctgatttata ttggtattcg ttgggtggca tattatccat tacgccatgc
     4081 aactcaagaa aatattgttc atgctgccaa gcagagcagt tatttcatgg aaaccatacg
     4141 tggtattcaa actgttaaat tatttgataa aaatgcacaa cgccatgcgg cttggctgaa
     4201 cttgtttatt gacacgatta acacaggttt aatgacacaa aaactttccg cgctgttcgg
     4261 ttttacgaat ggccttttgt ttggtatcgc taatattttg attgtgtatt ttggtgcagt
     4321 ttctgtgttg gacggtgttt ttaccgttgg ggcgttaatg gcgtttatgt cctacaaaag
     4381 tcagtttgag agtaaagcag ggtcattgat tgaccagttt gtgcaaatca aaatgctagg
     4441 attgcacgct gaacgtttgg cggatattgt gctagaagaa acagaaaacg agcagaacaa
     4501 tgatttcagg ctgcctgaaa atttagaaca ctttgatatt caaatagaaa atgtttcttt
     4561 tcgctatgct gaaaatgaac catacatttt gcaagatttt agtttaatca tcaaacaggg
     4621 aacagccatt gcgtttgctg gacattcagg ctgtgggaaa tctacgctca ttcaaattct
     4681 gacaggcagt ctgaaacctg aaagcggtca tgtgttatta ggtgggcatg acattcatgc
     4741 gttaccacct gcatttatcc gcacatggag cgcaagtgtg atgcaaaatg atgtgttatt
     4801 tactggctca atcgcagaaa acatcagttt ttttgatgat acgcccaata tggaaaaaat
     4861 cgcattttgc gctcaaatgg cgaatattca tcatgaaatt gtcgctatgc ctatggcgta
     4921 tgaaacattg attggcgaca tggggagtgc attgagtggt gggcaaaagc agcgcatcgt
     4981 actagctcgt gctttatatc gtgaaccaaa aattttgttt ttagatgaag ccagtagcca
     5041 tttggacatc aacaatgaac gtgtgattaa cgataatcta cgccaactca acattaccaa
     5101 aatcatggtc gcgcatcgtc aggaaacttt aaatactgct gatagcgtag tttatttaga
     5161 caaggtagcc tgaaatgaca ttatttcgcc ccgaagtatt ccaagctaaa aaaaatcgct
     5221 ggacagggca aattgtttta gtgcgtcctt tttccttgca gtttttaaca tttttcgcgg
     5281 tctcattagc ggctatttta gttgcttttt taatttttgg gagctatacc aataaaacca
     5341 ccgtaacagg acaacttttg ccgacaacag gggtagtacg cgtatattcg caggatatgg
     5401 gggttattgc tcatcaacac gttatgaatg gcgattttgt gaaaaaaggc gatgtgttat
     5461 ttacattatc tacttcacga aatgacaata atggcagcat acaagcacga ttactcgccg
     5521 aagctgaatt aaaaaaatca ctatcagaac aagaaattat tatgaaaaaa cgtgttcatg
     5581 cagcagaaaa aaccgcacaa gaaaacacgg tacaccgttt tcagaaccaa atgcaacacg
     5641 ttagaaatca aattatcatg caagaaaaac gtattgcgat ttctgaaaaa atgttagaaa
     5701 aacagcgtta tttagccaaa atggacgcaa tttctgaatt agaaaaaaat agttatgaaa
     5761 ttgctttatt ggagctgaaa gcaggtttag ccgcttacca acgagaagca gataatcttg
     5821 ctcgggaaat aactgtacag caaagcaatc taaaaaatct gcctgaacag caagctactg
     5881 aaatcagtca attagaacgc gcagtatctg tctatcaaca agaaatattg gattatcaac
     5941 aacgtaacga acaaactatt cgcgcaacca tatcaggcta tgtcagttct attaacacgg
     6001 aaattggtca gcaggtagat actaacaaac tattgatgag tatcgtgccg aaagaaagtg
     6061 aattactggc taatttatat gtccctagtc gcgcaatcgg ttttgtaaaa ccaaatgaca
     6121 aggtaatttt gcgttatcaa gcatatcctt atcaaaaatt cggacacgca gaaggacacg
     6181 tcatttctat tgctcaaacg gcactagggg cgcaagaatg gacaaattta ggcaatattt
     6241 ttactcaaac agcacaagtg aatgagcctg tttatttaat caaagtaaaa ctggataatc
     6301 agcatattcg tatatatggc acggagaaga aattgcaaat tggtatgata ctagaagccg
     6361 atattttgca tgagaataaa cggttatatg aatggatact tgaccctttg tatcaagtaa
     6421 tgggaaaaat atcatagctg aattgctcaa tacattggag cgtatgagtg cccctaatta
     6481 tcaagcaaaa ccaatgtccg atgacttcca atttgttgtt aagcacaaat acacagtgtt
     6541 ttatgtaata atgaagacag agaagtgata caatttttcg ctccattttt gcttcctgaa
     6601 ttgctgcaat tgtatctgcg ttgtatgcga gaagtaaata ttttcgatgc atttgctgac
     6661 tttgtaccat aatacgtctt ccaaccagta tgataggctg gtttttctac atgccgatgc
     6721 cgtggccgtc gtcccgctcc atacgccgcg cctgccgctc ctgctctttg cgctggtctt
     6781 cccgctcctt ctgctctact tcccttatcc gctgcgcgcc gaaataaaac gcgattcgct
     6841 tggccgaagg gctgccgttc agggggaatt tttccagttc gatttgaacc gccttctgcc
     6901 ctattgtcgt ctgtgcttct tgggctgcct gcaaaaatac gttcctgaat tcttccatcg
     6961 gccgtttgcc gatttccctg cggacattgt ccagttcttc gttaaacctg tcgcgtactt
     7021 tgaccacgtc ggcggccaac ttgtcgctat actgcccttc cagtttttcg ttttctgttt
     7081 tcagccgccg gatttctcgc acgttcgctc catgccgttc gttgatgtgt tgctgcttct
     7141 cgttttcctt aaccagtttt gcgccgattt tacccatgcc gaaaaagcgg ttttcgcctt
     7201 ggtcttggac tgcccaatag tcgtgatgca gccgtttgtt gtcgtcttcc agcgccctta
     7261 ttttgtttcg gttttcgttc atgcggtatc gcgcattgat gtaccaatct ccgcgttcta
     7321 tatcgcgctc caagccgcgc atgtcgtcca gttgtttttg aaacagcctg tatatcgtca
     7381 ttagcttgtc gttcggcagc ttggcgataa agtgctcttc ctgtttcggc ggctctttcg
     7441 gtgtttcctg cgcctgcttt gcccgctgct cggcggcttg ggcagcccga ttataattaa
     7501 ttttatccca gaattcacaa tttgagacct ttgcaaaatt ccccaaaatc ccctaaattc
     7561 ctactaagac atttagggga tttctcatga gcaccttctt ccggcaaacc gcacaagcca
     7621 tgattgccaa acacatcgac cgcttcccac tattgaagtt ggatcaggtg attgattggc
     7681 aaccgatcga acagtacctg aatcgtcaaa aaacccgtta ccttcgagac caccgcggcc
     7741 gtcctgccta tcccctgctg tccatgttca aagccatcct gcccggacaa tggcacagcc
     7801 tctccgatcc cgaactcgaa cacagcctca tcacccgcat cgatttcaac ctgttttgcc
     7861 gttttgacga actgagcatc cccgattaca gcaccttatg ccgctaccgc aactggctgg
     7921 cgcaagacga caccctgtcc gaactgttgg aactgatcaa ccgccaactg accgaaaaag
     7981 gtttaaaaat agagaaagca tccgccgccg tcattgacgc caccattatt cagaccgccg
     8041 gcagcaaaca gcgtcaggct atagaagtcg atgaagaagg aaaagtcagc ggccaaacca
     8101 caccgagtaa ggacaaagat gcccgttgga tcaagaaaaa cggcctctac aaactcggtt
     8161 acaaacaaca tacccgtacc gatgcggaag gctatatcga gaaactgcac atcacccccg
     8221 ccaatgccca tgagtgcaaa cacctgtcgc cgttgttaga agggatagct gaaagtacga
     8281 ccgtctatgc cgacaaaggc tatgacagtg cggaaaaccg
//
LOCUS       DAXHKX010000079         8249 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHKX010000079 DAXHKX010000000
VERSION     DAXHKX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.079"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(152..1084)
                     /gene="cysK"
                     /locus_tag="ACFMWZ_001298"
     CDS             complement(152..1084)
                     /gene="cysK"
                     /locus_tag="ACFMWZ_001298"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC5935256.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1278..1883)
                     /locus_tag="ACFMWZ_001299"
     CDS             complement(1278..1883)
                     /locus_tag="ACFMWZ_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935257.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1880..2731)
                     /gene="dapF"
                     /locus_tag="ACFMWZ_001300"
     CDS             complement(1880..2731)
                     /gene="dapF"
                     /locus_tag="ACFMWZ_001300"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC5935258.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2868..3164
                     /locus_tag="ACFMWZ_001301"
     CDS             2868..3164
                     /locus_tag="ACFMWZ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935259.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3178..3846
                     /locus_tag="ACFMWZ_001302"
     CDS             3178..3846
                     /locus_tag="ACFMWZ_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC5935260.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3918..6038)
                     /gene="pnp"
                     /locus_tag="ACFMWZ_001303"
     CDS             complement(3918..6038)
                     /gene="pnp"
                     /locus_tag="ACFMWZ_001303"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC5935261.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVREIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6275..7138)
                     /locus_tag="ACFMWZ_001304"
     CDS             complement(6275..7138)
                     /locus_tag="ACFMWZ_001304"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC5935262.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7337..8200
                     /gene="rfbD"
                     /locus_tag="ACFMWZ_001305"
     CDS             7337..8200
                     /gene="rfbD"
                     /locus_tag="ACFMWZ_001305"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC5935263.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPYAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2002 a         2374 c         1889 g         1984 t
ORIGIN      
        1 aaaatatttc cctcaagcga tgcggcgaca ccaacacctg attaaaacac aaccggcgtt
       61 ttcggcatat atgcaaaaac gcatttccca gcacggcgtt acaccgatac cgaaaaatgc
      121 gtctgaatgt ttcgacaatc cgattaaagc attatgccaa atctgcaaaa agtggcgtag
      181 agagatagcg ttcgccataa gaaggcagca gcacgactat cagcttgcct tcgttttcag
      241 gctgtttggc aagctgcaac gcactccaaa ccgccgcacc ggaagaaata cccaccaaaa
      301 tgccttcttt ttccgctatt gcgcgggcgg tttcaaaagc cgcttcgttc gacactttgg
      361 taatgctgtc gtaaattttg gtattcaaaa cggtcggaat aaagcctgcg ccgatgcctt
      421 gaatcgggtg cgggcctttt tcgccgccgc tcaatacggg tgaagcttca ggctcgacgg
      481 caaccacttt aacttcgggt ttgtattttt tcaacacttc gcccacgccg gtaatcgtac
      541 cgcccgtgcc gacgccggca acgaagacat cgactttgcc gtccgtatcc cgccaaattt
      601 cctcggcggt tgttttgcgg tggacttcgg ggtttgcctc attgtcgaac tggcgcggca
      661 taaaataagt gtcgggatgc gcgtccacca aggatttcgc tttggcaatc gcgcccgcca
      721 taccttcggc ggcaggggtc agaatcagct ccgcaccaaa cgcgcgcaac agcattttgc
      781 gctccttact catgctttcc ggcatggtaa tcgccagctt gtagccgcgt gcggcacata
      841 ccattgccaa accgacaccc gtattgccgc tggttgcttc gacaatgacg gtgtttttgt
      901 tgattttgcc cgctttttcg gcaccctcga tcattgcttc ggcaatgcgg tctttgacgc
      961 tgctgcctgg attgaaaaat tccagtttca cggcaacctc cgccttcaaa ccttcggtca
     1021 gacggttcag tttgaccaaa ggcgtgttgc cgattagttc ggtgatgctg tttgcaattt
     1081 tcatcttctg ctcctgaata ataaggtttc gacagtaagg cgcaatcccc ataggcatcc
     1141 tgtccgattg caggggattt gcgccgaaac ataaacaaat ttcggatatg gctttatcct
     1201 aaacctatgc cgcctaaaaa accaatatcc aaaatttctt tctttataac tttttgttcc
     1261 gatacggccg caccgtatta tcttccaatt tctgaaagca gcagcaccag taccgagacc
     1321 tgcatcaacg ctgctgtcaa cccgccgagt cgcagctgcg agccgccgaa cgttgacggc
     1381 ggctgccggt ctataccgca gcggtggaca taggcaaccg ccatcagcac aaactgcaac
     1441 acgaaccaat aaaccggctt cagcgcataa agtttgagca gtgccgcaaa cacaaacact
     1501 tgaaccgttc ccgaaaagca ataatgcacc aacgcacata ttccgacaaa agcaagcgtc
     1561 atcgatacat tactgacggc aaacagcccg agcagaggat gttcggggtc tgcctgccat
     1621 ccgtttccat ccgttttccg gttccaatgc ccgatgaaaa aaaatatgct gcccaaagtc
     1681 aggtaaaaat gggggatgaa caagggcgcg gcgcgggtca ttccccatat gccgggcatc
     1741 agctttgccc ccaaaaccga tatgcccagc cacagcataa tactcgccca aaaccattgc
     1801 aacattcctg cgcggaatat cagaaacaga aaaaccgccg catacagtaa ggcagacaaa
     1861 aaatccaaat gcagcaaaat catgaatacg ccaactcgcc ttcaaacacg gtttccgcag
     1921 ggccggtcat catcacatcg ccgccgcagg cccattcgat atataaagtt ccgcccggca
     1981 aaatcacctc taccgttttc ccttcatcca acaaccccag acggataccc gccaccacag
     2041 ccgcacacgc gcccgtaccg cacgcttggg tttcgcccac gccgcgctcg aacacgcgca
     2101 aacgaatcgc ggttcggctg acaatctgca taaagccgac gttgacgcgt tcgggaaact
     2161 gcctgtgcgg ttcgataagc gaaccggttt cgcgcaccgg cgcgcattcc acgtcatcga
     2221 ccacaatcac cgcatggggg ttgcccatat tgacgcagct gacaggctga atgccggatt
     2281 cgagatgcac cccgtaaata caggcatcat ccccctcgcc cgattcgggg acaaacggta
     2341 tttcagacgg cataaacttc ggtttgccca tattgaccgt aaccataccg ttatcggaca
     2401 atttcggaaa aataacgcca tttgccgttt caacacaaat ttctttctta tcggtcaaac
     2461 ccttgtctgc aacaaaacgg gcaaaacaac gcgctccgtt gccgcattgc ccgacctcgc
     2521 tgccgtcggc attgaaaata cggtaacgga aatccacgcc ttcggtttcc gaacgcccga
     2581 ccaccaaaag ctggtcgaag cccacgcccc ggaaacggtc cgcccatgcc gcaatcggcg
     2641 cgtcctcggg ggtaaaatcc tgactgaccg cgtcaatcac cataaaatcg ttgcccaaac
     2701 cgtgcatttt ggtaaatttc aaagtgttca tattgttccg aaatcccgta tcgtctgttg
     2761 cttccgaatc cgaaatcccc cgcaccgaag ggaaatgctt ccatctcccg ccgcctgagg
     2821 attaaaatca gccattatac tgtttgttac cccttacaag aaacaccatg ttccgcatat
     2881 tactgttgct tttcccttgt gcaatccttg ccgcctgcac ccgaaacata ccggcaaacc
     2941 cggccgtttc aaattgcgcg catcttcaaa accgcaccat cctgtatttt gacgcacacg
     3001 gcaaacccgc cgacggcgca gtaaagggcg gattttacaa aattatcgaa atacagggtg
     3061 caaactgcat ccttattcaa gactttaaca gcacgggcaa tgccctcggc ggatcctatg
     3121 tgccggactg ccgcaaatca tcccttttcc cgaaaaatat ttgacttcaa actgcctatg
     3181 aaccacgaca tcactttcct caccctgttc ctactcggtt tcttcggcgg aacgcactgc
     3241 atcggtatgt gcggcggatt aagcagcgcg tttgcgctcc aactcccccc gcatatcaac
     3301 cgcttttggc tgatcctgct gcttaacaca ggacgggtaa gcagctatac ggcaatcggc
     3361 ctgatactcg gattaatcgg acaggtcggc gtttcactcg accaaacccg cgtcctgcag
     3421 aatattttat acacggccgc caacctcctg ctgctctttt taggcttata cttgagcggt
     3481 atttcttcct tggcggcaaa aatcgagaaa atcggcaaac cgatatggcg gaacctgaac
     3541 ccgatactca accggctgtt acccataaaa tccatacccg cctgccttgc ggtcggaata
     3601 ttatggggct ggctgccgtg cggactggtt tacagcgcgt cgctttacgc gctgggaagc
     3661 ggtagtgcgg caacgggcgg gttatatatg cttgcctttg cactgggtac gctgcccaat
     3721 cttttggcaa tcggcatttt ttccctgcaa ctgaaaaaaa tcatgcaaaa ccgatatatc
     3781 cgcctgtgta cgggattatc cgtatcatta tgggcattat ggaaacttgc cgtcctgtgg
     3841 ctgtaagccc cattcattcc cgatacccgt aaaaataccg tctgaaacct gttcagacgg
     3901 cactttcacc ctaagcgtta ctcggcggca ttttcctcac gggcaggcgc gtccagcagg
     3961 gctttgatgg acagacggac acggcctctg tcgtccactt ccaatgcttt cacgttcacc
     4021 acctgaccga cttgcaggta gtcgccgaca ttgcgtacgc gctcgtgggc gatttggctg
     4081 atgtgtacca aaccgtcttt gcccggcatc acactgacaa tcgcgccgac attgttgtcg
     4141 aggattttca ccacagtgcc ttcgtacact ttgcccactt ccacttcggc agtaatctgc
     4201 tcgatgcgtt ttttcgccgc atcgccggct tcttgagtgg ttgcggcaat ggtaatcgta
     4261 ccgtcttcgg caatattgat ttccgtaccg gtttcagcgg taatcgcacg gatggtttca
     4321 ccgcccttac cgataacttc gcggattttg tcttggttga ttttcatcgt gaacaagcgt
     4381 ggcgcgtgtg cggacagctc ttgcgggccc gcaacggcgg ctttcatctg atccaagatg
     4441 tgcagacgcg cttctttggc ctgcgccaaa gcgatttgca taatctcttt ggtaatgcct
     4501 tggattttga tgtccatttg cagcgcggta acgccttcgg tcgtaccagc cactttaaag
     4561 tccatatcgc ccaagtggtc ttcgtcgccc aaaatgtcgg tcaggacggc aaacttattg
     4621 ccttccagaa tcagacccat cgcgataccg gcaacgtgtg ctttcaaagg cacgccggcg
     4681 gacagcaggc tcaagcagcc gccgcagacg gaagccatag aggaagagcc gttggattcg
     4741 gtaatttcgg agaccacgcg catggtgtag ctgaaatctt caggtttcgg caatacggcc
     4801 aacaatgcac gtttagccaa acggccgtga ccgatttcac gacgttttgg cgcacctacg
     4861 cgacccactt cgccggtaga gtatggagga aagttgtagt gcagcatgaa gcggtcggtg
     4921 tattcaccgg acagcgcatc aatgatttgc tcgtcgcgtg aagtacccaa agttgcaacc
     4981 gccaaagctt gagtttcgcc acgggtaaac aatgcagaac cgtgcgtgcg cggcaatacg
     5041 ccggtttgaa tgttcagcgg acgaacagtg cgggtgtcgc gaccatcaat acgcggttgg
     5101 ccgtctaaaa tttggctgcg gacaacatcg gcttccaagt gtttgaaaat gcctttgatt
     5161 tcgttggctg ccaaagtgtc ggtttcctcg gtaatcaagg cttcttttac agcactccaa
     5221 gcttcgtcca atttggcaga acgcgcttgt ttttgacgga ttttgaatgc ttctttaatg
     5281 gtttcaccgg caatttcgcg tacttttgca accaattctt cattggtttc aggtgctttc
     5341 caatcccaaa cttccggatt gacttcatcg gcaaactcat tgatggcgtt aatggccact
     5401 tgcatttgat cgtgaccgta aaccaccgcg cccaacatca cgtcttcggg caggattttg
     5461 gcttcggatt ccaccatcaa cacggctttg gaagtaccgg cgaccaccaa gtccaattgc
     5521 gatttcgcca attcggcttt agtcgggttc aaaacgtaca cgccgtttac ataaccgacg
     5581 cgtgccgcgc cgatcgggcc ggcaaacggt acgccgctca acaccagcgc ggcagacgcg
     5641 cccaacattg caggaatatc agaatcgatt tcaggatcga cggacacgac catcgctacg
     5701 atttggatgt cgtggtagaa accttcaggg aacagcggac gaatcggacg gtcgatcaga
     5761 cggctggtca ggatttcttt ttcgctttgt ttgccttcgc gtttgaagaa accgccggga
     5821 attttgcctg cggcgtaagt gcgctcgagg taatcgacag tcagagggaa gaagtcttgg
     5881 ccttctttca cttctttgtt ggtggtaacg gcaaccaaaa ccacggtgtc gcccatagag
     5941 actttaacgg cagcggcggc ttggcgggca atttcgccgg tttccaaagt aacggtgtgg
     6001 ttgccgtatt ggaaagtttt aacgtgtttg tcgaacatta ttgttccttt caaaataccg
     6061 cactgctaaa acactaataa tgcacactaa aatccgaatg tgcatagtta gggtttcaga
     6121 ccgtgcggca ggttgtaaac aagctttcag acgacatttc ggacgctgaa agcaattcca
     6181 aattataaag gcaaccatcc ttaaaatcca gtattgatac aataaaaagg ccgtctgaac
     6241 catatttctt tcagacggcc ttcaaactta aaaatcaatc ctgagtcagc aaagtcaatg
     6301 cttcccgata cttctcgaca gttttctgaa tcacatcggc aggcactttc ggcgcagggg
     6361 cttttttgtt ccaaccgctt tgttccagcc agtcgcggac gaattgtttg tcaaaagacg
     6421 gcggattggt gccgactttg tattggtcgg caggccaaaa acggctcgaa tcgggagtca
     6481 atacctcatc catcagcgtc agcgtaccgt ttgtatccaa accgaattca aatttggtat
     6541 cgcaaatgat gatgccgcgc gatttggcat attccgccgc ttctgtataa agctgaatcg
     6601 ctttggcgcg cacttccgcc gccaattctt ttccgataat gcgttcgcat tcttcaaagc
     6661 tgatgttttc atcgtgatcg ccgactgcgg ctttggttga gggcgtaaaa atcacttcag
     6721 gcagttgttg cgcttcctgc ataccttcag gcagttgaat accgcaaacc gagccggttt
     6781 tttgataatc tttccaaccg ctgcctgcca gataaccacg cacaatcgcc tctactttca
     6841 ccggagtgag ctttttagcc acgacggcgc gtttctctaa agctttggct tcgttttcag
     6901 gcaaaacatc gtaaaccgtt tgaccggtaa agtggttggg cataatatgc accagttttt
     6961 taaaccaaaa attggaaatc tgcgtcagaa tctccccttt gctcggaatc gggtcgtcca
     7021 aaatcacatc gaatgcagac aggcggtcgg aagcgaccat cagcatacgt ttatcgtcga
     7081 tttcatataa atcgcgcact tttccaaaat agatctttac caaaccaatc tcactcattt
     7141 cgccccccct gaaaatatct tgaaaatacc gacccgacac ccgacaggtt tgaatcacaa
     7201 accaatattc tagccgaagt cggcgcaaaa caatacccat ggcacaaaaa accaacccgt
     7261 caaccgtcgg caaagttttg gcactataat accgacagca agtcctacaa tacactttta
     7321 ccaaaggaaa tacctcatga gaatcctatt gacaggctcg aaaagccaac tggcacgctg
     7381 cctgcgcgac cgtcttccgg aagactggga aaccattgcg acggattccg catccctaga
     7441 cattaccgat gccgatgccg tctgcaacat ggtcaaaagt ttccaaccct acgctattgt
     7501 caacacggct gcctatactg ccgtcgacaa agcggaaggc aatgcggcag cggcatttgc
     7561 cgtcaatgct tccgccgttt acaaccttgc cttggcagca catcgcgccc atgcccgatt
     7621 catccacatc tcaaccgact atatctttga cggtaaaggg aaaaggccct atcaggaaag
     7681 cgactttacc aatccttcca atgtatacgg acaatccaaa accgcaggcg agctgctcgc
     7741 actgtctgcc aatcccgaca gccttatcct gcggacttct tggctgttta gcgaatacgg
     7801 ggacaacttt gtccgcacca tgctgaacct tgcgcgggaa cgttccccgc tgtccgccgt
     7861 ccacaaccaa atcggctgcc cgacctatgc cggcgattta gctgccgcca tcatccgcct
     7921 tttgcagcaa acgtcgcccg tgcgcggcat ttaccactac gccggcggca aatccgtatc
     7981 ctggtacgaa tttgcccaac atattttcca agcggcatcg caacagcaga catccttccc
     8041 cgttcccgaa ttgactgccg tttcagacaa ggaatatccg accgccgccc ccaggcccgc
     8101 atacagcatt ttggactgcc gcaaaatcga aaacgacttc agcatcaaac cgtcagactg
     8161 gcaaaaagcc cttgcacagg tcgtttccaa gctgctctga tgccgcccgc ctctccgttt
     8221 ccgccgtcaa gcaccgcctt ggcggtttt
//
LOCUS       DAXHKX010000080         8215 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHKX010000080 DAXHKX010000000
VERSION     DAXHKX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.080"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(263..2953)
                     /gene="glnE"
                     /locus_tag="ACFMWZ_001306"
     CDS             complement(263..2953)
                     /gene="glnE"
                     /locus_tag="ACFMWZ_001306"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC5935264.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFIYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVLTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVGKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTSEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(3133..3468)
                     /locus_tag="ACFMWZ_001307"
     CDS             complement(3133..3468)
                     /locus_tag="ACFMWZ_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935265.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            complement(3722..4195)
                     /locus_tag="ACFMWZ_001308"
     CDS             complement(3722..4195)
                     /locus_tag="ACFMWZ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC5935266.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4248..4529)
                     /locus_tag="ACFMWZ_001309"
     CDS             complement(4248..4529)
                     /locus_tag="ACFMWZ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935267.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4616..4939)
                     /locus_tag="ACFMWZ_001310"
     CDS             complement(4616..4939)
                     /locus_tag="ACFMWZ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935268.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5078..5257)
                     /locus_tag="ACFMWZ_001311"
     CDS             complement(5078..5257)
                     /locus_tag="ACFMWZ_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935269.1"
                     /translation="MPSERTFCRSDGIFISFSCLPPFENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            complement(5273..6280)
                     /locus_tag="ACFMWZ_001312"
     CDS             complement(5273..6280)
                     /locus_tag="ACFMWZ_001312"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC5935270.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     Q"
     gene            6512..6748
                     /gene="acpP"
                     /locus_tag="ACFMWZ_001313"
     CDS             6512..6748
                     /gene="acpP"
                     /locus_tag="ACFMWZ_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC5935271.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6904..8151
                     /gene="fabF"
                     /locus_tag="ACFMWZ_001314"
     CDS             6904..8151
                     /gene="fabF"
                     /locus_tag="ACFMWZ_001314"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC5935272.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1908 a         2168 c         2107 g         2032 t
ORIGIN      
        1 tagaatgcgc tattttaatg gaaacggcgg ggttttaaac ggttcttatt tgtttgtttt
       61 gatttttgct tgggtaaaaa atcccatcat tcccacaaaa acagaagcct gaaatcccgt
      121 cattcccgcg aaagaggaat ccggtttttg ggtttcagcc attcccgata aatcgcttta
      181 gctctgccgc aaccttaaaa cggcacggta cgaaaatacc gtctgaaacc cagattgtca
      241 ggcttcagac ggcatttttt gttcaggcgg ttgccgcttc ttcgccgaac acttcccgcc
      301 acaatttcct gacattgccg taatgtgcca acagttcgcc ggttacttcg gtttttgccg
      361 cgtcgcgcag tttggtgttg tgctgctgcc ggcggtagaa gcgataggcg gtgcggcttt
      421 gtccggcgag ggttttgtca atcaaaccgc agtcggcgga gatgtttaag agggcgatgt
      481 tgccgtagtt gtccaagagt tgcggatact ggcgggcatg ggcaagtatc agatattgga
      541 cgataaattc gacatcgacc acgccaccgc gcgcgtattt gacgttgctg tcggcaggcg
      601 ggtgggtggg gaacattttt tcgcgcattt cgatgatttc gcctgccaag gcggtttggt
      661 cgcgttcggc ggtgaggatt tcggtgcgga tgcggtcgaa ggccgtctga atttcggacg
      721 tgccgcagat gaagcgggcg cgggtaaggg attggtgttc ccacgtccag gcgttttcgc
      781 gctggtattt ttcaaaggcg gcgatgctgt gggcgaggaa accggcgtcg ccattagggc
      841 gcaggcgcag gtcggtttcg tagaggctgc ctgcgccagt ggcggcggaa agccagttgg
      901 tcaggcgacg ggcgaggcgg ctgtacacgt cgcctgcgtc ggggtgggga tcgtcgtaga
      961 ggtagacgag gtcgaggtcg gaggcgtagc cgagttcttt accgccgagt ttgccgtagc
     1021 cgacgacggc gaattgcggt gtgtcgcggt gttttttggg catgtccgcc catgcgcaca
     1081 gcagggcggc ggcgaggatg gtgtcggcga gggcggagag ttggtcggag agggattcta
     1141 ccgtccacag tccggcgagg tcttggacgg cgagacggaa gacttgggcg tgctggaagc
     1201 ggcgcagggt gtccatttgc gcttcagtat cgccgccgca ggctttgagg tcgtctgaaa
     1261 gggcggcggc gagcgcctgc caatcaaacg cggtatccaa aagctgcgcg ctgatgagtt
     1321 cgtccaacaa aatcggatat ttgttcagat acgccgccac ccaagaactt tggcccataa
     1381 tctgcgccag ttgcgccaag gtttgcggat gttcgttgag gaaggcgaga taggcggatc
     1441 ggcggctgat gttttcgaga aaatccaaca gccgcatcaa tgtatcggtc gggttgcttt
     1501 gcgctgccgc cgcctgtacg aacagcggca caaccgcatc gaaacgcggc tgggcgtgtg
     1561 cggaaagatg gcggtattta tggccgtggc ggatttggtc gagccttgcg gcgacggttt
     1621 cggcatcgaa cccgtgcgcc ttcagacggc atcgccgccc ttcttcgtcg ggtttgtcct
     1681 gccatgccca ttgccattcg ctgttgtctt gcgtttgctc ttcgggttcg ctcaaaattt
     1741 cgttgaacaa ctgattgact ttgttccgat gaacattgag accgtctgaa aaagcggaat
     1801 aactgtcgaa acccatgctt tcagcgagca gttgccgctg ttcaggcgaa gtgggcaggg
     1861 tttgggtttg ttggtcgtcc cagtattgca ggcggtgttc gacgtcgcgc aggaagcggt
     1921 aggcggcaag cagggtttcg acgttttcag acggcatgat cccggtttcg gcaagtttct
     1981 tcagcgtttc ctgcgtgcct ttcagttgca gcgcgcgcat ttgtccgccg cgtatcatct
     2041 ggaaaatctg ggcgataaat tcgacttcgc ggatgccgcc cgcgccgagt ttgatgttgt
     2101 ccgccatccc ttttttgccg acttcgctgc ggatttggcg gtgcagctta cgcatcgcct
     2161 catacgcgcc gtaatccaga tattttcgga acacgaaggg gcgcaccagc gatttgatgc
     2221 cgttcggata cggcgtaagc acgcggcctt tgcaccacgc ataacgttcc cattcgcgcc
     2281 cctgcgtaat caggtattgc tccagcgcgg tttcgctcaa taccagcgcg cccgaatcgc
     2341 cgtccggccg cagccgcata tcgacgcgga acacctgccc gtcggcggta atgtcattca
     2401 gcaacgcaat cagtttctgc ccgactttgg tgaaaaattc ctgattgccg cgttcgcgcc
     2461 taccgtcggt atcgcccgat tcgggataga tgaaaatcaa atcgatgtcg gaagacacgt
     2521 tcaactcata gccgcccgcc ttgcccatcg ccaccacact caaatgctgc ggcgatttcg
     2581 tatcacgccc gatcggcgtg ccgtacatat cccgataata ggcgtaggca aaatccagcg
     2641 cggtattgac ggcaaaatcg gcaaacagcg taatcgtgcg ggttacttcg ttcaaatcgc
     2701 tgatgcggtt gatatcgcgc acgataatct gcgacaccac ataacggcgc aacccgcgca
     2761 actgccgcgc caattcttcc tcgttttctt ccgcacggat tttgtcccag tcggcaaagg
     2821 cttggaaatc cgcttcggtc aaaaccttgt ccaacatggg caggaaaatt tcaggcttga
     2881 gtttgccgtt gtcgagctgg cgggcaagga acagggaatg gcggcgggca gtgtcgaggc
     2941 ggttgtcgga catttcggat tccgtttgaa aaaatggcag gaagatgatg gcggattcac
     3001 ttcaaatcag gacagggcga ggggatgccg tactggttta aagtcaagcc actatatcat
     3061 agcaaccttc gatgtcgtct gaaagccgga taacgcttca gacggcatcg gattgacagg
     3121 tttaatccgc gcttacggac gctttttaaa tataaataaa gaaatctcgt ctaacgcatc
     3181 ttcggcaaaa ccttgaaaat ccggatcacc ttgttttgag agcaataaag caatcacttt
     3241 atcgcaatcg ccgatatttt catgcatcct ggcgagatgt ccgaaacagg ttaatgacaa
     3301 tctggcaata tccaagtctt tatgtttcga caactccgtc aacttctcgg aaatccaggg
     3361 gatttcatcc tcctccgagc tgaaggtcat accgagcata ccgtcaataa cactctgttt
     3421 attttcagaa taaatcatct tttctacttg ttccttatca accgtcattt tttaattttt
     3481 ccttttaaat taaccaatcc tgctttcttt aaagcatttt tgccgggcgg caggattgtt
     3541 atcaaacatt tgcacccggt ccggcaaaac gatttcctta tcggtttatc ggcaacgatg
     3601 cgggcggttc gggttttgcc gcttccggaa tgcagcccgc ccccgctttc aaatcttccg
     3661 ctctaatccg tttgccgtct gaaggccgga taatgcttca gacggcatcg gattgccggt
     3721 tttaaccctg ccctccgccg ttgttcaaat agtgttccca gtgagaatat aaccgggcaa
     3781 tattttcttc ataggtgtgg gcagaaggaa tataaccgtc tttgggcggc atatacctga
     3841 caaatgattc tatttcttcc ggataatcaa aatctgcata aattttttca acttccccca
     3901 aaggatcctc aaattcttct tttctatgga atacatcgct taagaggacg tacagccaat
     3961 ttttagggga ataatccctc atttccgaag ccaaatcttc cagaacaggc tttaattcaa
     4021 gaatattgga tttgttgatt aaagatagtt tgaacacacg ttcatcatga tctgaaagcg
     4081 tcatttttcg ggcataagct gccacatcag cccaacccaa gtatttattc ccataccccc
     4141 acagaatatc cttccaagaa agacggactt tttgcgcggt taaatcaagg ttcatttttt
     4201 actccgattg cctgccccgt cattccgccc cttccgattg acgcctgtta cgaaaagtct
     4261 tccaaatgac ggctgattgc ctcttcaaca gtcggatgcc aatccataaa tgaagcctgc
     4321 aattcctcta aaaccaactc attgaataaa tcatccaaat tccgccaatc cgttttttca
     4381 tccggataga tgaaatttgc cataacgaca gtatgtttgt tttgggaata atccgataag
     4441 gcgcaaatca tcagataccg ccccgaatgc tccagataaa aaattcgttt ttccaaagga
     4501 aaccggttat tccgttgccc gttttccatt tttcatcctt tttaacgtat cggcttccgc
     4561 gtccttcaaa ttgagttacc tgccttccgc caagcaaacg cccgcgcccc tttcatcatt
     4621 tgaatatccg tttgaccata tttaccagat aactatcttc tttatataaa tcaatcagca
     4681 aaggcacgat acccggctca taaaataatt ctgtcgtatc agatgtttct tttaacgcgg
     4741 aaattatctc caacagttca tacccgtatc ggggataagc aaacaaatag gggactgctt
     4801 cccgcaaaca tgaaaatgct tcgtaacttt gcatttccca tatatgttca tctttaaaat
     4861 cactcaaata ccattcatcc aattcacttt ccggatcgga tatcaatcgg ataaattcat
     4921 ttagttcgtc cttaaacata tcaaatcctt aaatataaaa tcttttgatt atccgccgta
     4981 tccttatttt ccacatcatg aatttcaacg ccgttcatat tttgcgtttt ctcaaaccgt
     5041 ccaccacggc ataaaattat tttttcatat caattattta tatgtttaac aacttaccgc
     5101 cggacttgct ttggctgcgt atcaagattg tccggttcgc ggcgggcgtt tgctttgttt
     5161 caaacagggc ggcgcgtaag ggattttcaa agggcggcag gcaggaaaag gaaataaaaa
     5221 tgccgtctga acggcaaaac gtgcgttcag acggcatttt gtgcgggacg cgtcattgcg
     5281 ccaaagcctt caaacattct ttgaccaatg ccggaccttt gtatatcaat ccgctgtaca
     5341 cctggacggc agttgcgccc aagcggattt tctctgccgc gtccctgcct tccataatgc
     5401 cgcctacgcc gataatcggc agcttgccgt cgatgtggtc tgccaacagc ttcaacaccc
     5461 gattactttt ttcatgaacg ggcagcccgc tcaaaccgcc ctgctcgcct gcgagcggat
     5521 ggctgccgag acttgatttg tcgatggtgg tattggtagc gatgatgccg tccatttcga
     5581 cggatttgac aacgtgggcg atgtcttcga tttgtgcttc atccaaatcg ggggcgattt
     5641 tgacggcgag cgggacgtat ttcccgtgta cagaggcaag ctgtgcctgt ttgtttttca
     5701 aagcctcaag caatgcgctc aactcgtcgc caccttgcag cgcgcggagg tttttagtgt
     5761 tgggcgagga aatattgacg gtaatgtaac ttgcgtgtgc gtaggctttt tcgaggcaga
     5821 ttaaataatc atcggcagcg ttttcaatcg gtgttacagc gtttttgccg atgttgatgc
     5881 ccaatacgcc gctgaattta cttttttcga tgttttgaat catggcatcg atgccgtggt
     5941 tgttgaagcc catgcggttg atgatgcctt gatattcggg aacgcggaag aggcgcggct
     6001 gcgggttgcc gggctgcggg ttgggcgtta ccgtgccgat ttcgatgaaa ccgaagccga
     6061 gcgcgcccaa tgcgtctata tattcgccgt ttttatcgag tccggcggca agcccgacag
     6121 ggttgggcaa atccaaaccc atcagtttta caggtttggt acggttgccg gttacgggaa
     6181 tcagacccaa tttataaacc atgttgagcg cgtcgagcgt gaagtggtgg gctttctcgg
     6241 catcgagtgc aaacaggatg cggcgggcaa gtggatacat gacagggctt tctgcttcag
     6301 atggaaatgc cgtcatttta accgaaatcc cgtcaaacat acggcacaca ggtcaaactt
     6361 gaacgacaaa acccgccgca cccaaaaatg tacaaatcgg caaatattga tataatagtc
     6421 attagataga aaactctatt aaaagtcggt acaataccac cgatttattt cccttaaaca
     6481 acctgccgca aggcataaag gaacgactga tatgtcaaac atcgaacaac aagttaaaaa
     6541 aattgttgct gaacaactgg gcgtaaacga agccgacgtg aaaaacgaat cttctttcca
     6601 agacgacttg ggcgcggatt ctttggacac cgtggagctg gttatggctt tggaagaagc
     6661 attcggctgc gaaatccccg acgaagatgc cgaaaaaatc accaccgtcc aactggctat
     6721 cgactacatc aatgcccaca acggctaacc ggtcgtcgcc cgacacaaca gcctctgctg
     6781 caccgcgcaa tagaggcttt tcccttatat ggcaaactgt ttgaacccct gccgcaaatt
     6841 acagacggca tctgcacgac agccgcatat gcccccattc cgacaaccaa cagcgaggtt
     6901 atcatgagtc agagaagagt agtcattaca ggcttaggtc aggtttcccc tgtcggcaac
     6961 actgtcgcag aggcttggga caccctgctc gccggcaaaa gcggcatcgg cgcgattacc
     7021 cgctttgacg catccgacat caacagccgt gtcgccggcg aagtgcgcgg tttcgacatc
     7081 ggacaataca tcagcgcgaa agaagcgcgc cggatggacg tattcatcca ctacggcatt
     7141 gccgccgcat tgcaggcaat cgccgattcg ggcttggacg atgtggaaaa cctcgacaaa
     7201 gaccgcatcg gcgtgaacat cggttccggc atcggcggac tgcccagcat cgaggtcacc
     7261 ggcaaagccg taatcgaagg cggcgcgcgc aaaatcaacc ctttctttat ccccggttcg
     7321 ctgatcaacc tgatttccgg acacgttacc atcctcaaag gctaccgcgg cccgagctac
     7381 gggatggttt ccgcctgtac caccggcgcg cacgccatcg gcaattccgc ccgactgatc
     7441 aaatacggcg acgcggacat aatggttgcc ggcggcgcgg aaggcgcaat cagcactttg
     7501 ggcgtgggcg gttttgccgc gatgaaagcc ctctccaccc gcaacgacga ccccgccacc
     7561 gcttcccgtc cgtgggacaa aggccgcgac ggcttcgtta tcggcgaagg cgcgggcata
     7621 ttggtgttgg aagaattgga acacgccaaa aaacgcggcg cgaaaatcta cgccgaaatc
     7681 gtcggcttcg gcatgagttc cgatgcttac catatcaccg cgccgaacga agaaggcccc
     7741 gcccttgccg ttacccgcgc gctgaaagat gccggcatca atcccgaaga cgtggattac
     7801 gtcaacgcgc acggcacgtc cacccccttg ggcgatgcca acgaaaccaa agccctcaaa
     7861 cgcgcgttcg gcgaacacgc ctacaaaacc gtcgtcagct cgaccaaatc catgaccggc
     7921 cacctgctcg gcgcggcggg cggcgtggag gccgtgtaca gcattttggc gatacacgac
     7981 ggcaaaatcc cgccgaccat caacattttt gaacaagacg ttgaagccgg ctgcgatttg
     8041 gactactgcg ccaacgaagc gcgcgacgcg gaaatcgacg ttgccatttc caactccttc
     8101 ggcttcggcg gcaccaacgg cacgctggtc ttcaaacgct tcaaaggctg attccgcaaa
     8161 gccgccgccg acatcgaaat gccgtctgaa accgtttcag acggcatttt tatag
//
LOCUS       DAXHKX010000081         8209 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHKX010000081 DAXHKX010000000
VERSION     DAXHKX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.081"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(162..1241)
                     /gene="apbC"
                     /locus_tag="ACFMWZ_001315"
     CDS             complement(162..1241)
                     /gene="apbC"
                     /locus_tag="ACFMWZ_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC5935273.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAIADSVQETLMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1452..1527
                     /locus_tag="ACFMWZ_001316"
     tRNA            1452..1527
                     /locus_tag="ACFMWZ_001316"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1485..1487,aa:Lys,seq:ttt)
     gene            1606..2115
                     /locus_tag="ACFMWZ_001317"
     CDS             1606..2115
                     /locus_tag="ACFMWZ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC5935274.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2458..2898
                     /gene="nadR"
                     /locus_tag="ACFMWZ_001318"
     CDS             2458..2898
                     /gene="nadR"
                     /locus_tag="ACFMWZ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC5935275.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3013..3513
                     /gene="hpaC"
                     /locus_tag="ACFMWZ_001319"
     CDS             3013..3513
                     /gene="hpaC"
                     /locus_tag="ACFMWZ_001319"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC5935276.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQPEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            3529..4224
                     /gene="murU"
                     /locus_tag="ACFMWZ_001320"
     CDS             3529..4224
                     /gene="murU"
                     /locus_tag="ACFMWZ_001320"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC5935277.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4455..4886)
                     /locus_tag="ACFMWZ_001321"
     CDS             complement(4455..4886)
                     /locus_tag="ACFMWZ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC5935278.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGKNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            5061..6737
                     /locus_tag="ACFMWZ_001322"
     CDS             5061..6737
                     /locus_tag="ACFMWZ_001322"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC5935279.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPRKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNGLNIDPK
                     RVLWRRVVDMNDRQLRHIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSSGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            6851..7942
                     /gene="ychF"
                     /locus_tag="ACFMWZ_001323"
     CDS             6851..7942
                     /gene="ychF"
                     /locus_tag="ACFMWZ_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC5935280.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
BASE COUNT         1849 a         2082 c         2373 g         1905 t
ORIGIN      
        1 acaaaacaga aaaccaaaat cagaaaccta aaattcgtca ttcccacgaa agtgggaatc
       61 cggaacgtaa aatctcaaaa aaaccgtttt atccggcaag tttgcccgcc ggcaggcgcg
      121 gggcttggga cggcattgct gttgcggttt cggacgcggc tttattcgac gacgatttta
      181 gggaaccggc tgctgaagtc tttgcctttg tcggcaatgc ccagggcgat ttggaatgcg
      241 gcatcggtgt agattcgggc gatggcgggg tgttcgtcga acagttgcgc cggtgtgccg
      301 ccgtccatgg cttcgcgcac gggcaggctt aggggaagct gtccgagcag ggggacgttg
      361 aggcgtgccg ccaaatcttt gccgccgtcc gtgccgaaca gtgcttcgct gtgtccgcag
      421 ttggagcaga tgtggacgga catattttcc aatacgccca aaatgggaat gttgactttg
      481 cggaacatat ccacggcttt gcgcgcgtct atcagggcga tgtcctgcgg cgtggttacg
      541 atgacggaac cggttacggg gatgcgctgg gacagcgtga gctggatgtc gccggtaccg
      601 gggggcaggt cgatgaaaag atagtccact tcgtcccact cgctttggaa catcagctgc
      661 tgcaatgcct gactgaccat cgggccgcgc cagacgacgg cttggccggt atcgacgaga
      721 aagccgatgg acataacctg tatgccgtct gaagattcga cgggaatgag tttttggttt
      781 ttctgatcgg gtttgcggtc gtccacaccc aacatggtcg gttggctcgg gccgtaaagg
      841 tcggcatcga gtacgccgac gcgcgcgccc atgcgcgcca ttgcggcggc aaggttggcg
      901 gtggttgttg atttgcccac gccgcctttg ccggaggcga cggcaatgat gtttttcaca
      961 cctttgatgg tggtaacgcc aggttggact ttatgtgtgc cgatttccgt gtccatggac
     1021 agatggatgt gtgtgtcgcc tgtttcgggc atcagggttt cttgtacgga atcggcgatg
     1081 gcggcggcga tgtgcgcgac agggaagccg aaatgcaggg cgatatggat gccgtctgaa
     1141 cgctgttcga ccgaacggac ggccttttcg ccgccgagcg tgcgtgccgt attcggaacg
     1201 gcgacggtgt cgaggagggt gcggatgttt tgtatgttca taatgtgtgt ttctgtgtgc
     1261 agtttgggaa acgggcggaa agtgtacatt gatttcgggc ggtttgcttt tccttgtgcg
     1321 aacttttaca cggtatgcgg cggattgtga aaatccgttg tcaaaatatc ccgtgcaaac
     1381 agtggtctgc aattaagcct gtaaaatttg cttgacgagg taggatggtg ctgtataatg
     1441 cacggcttat cgggtcgtta gctcagccgg tagagcagcg gacttttaat ccgttggtcg
     1501 agcgttcgaa tcgctcacga cccaccagat aaccggaagc caagttttga gacttggctt
     1561 tttgtttgcc tgccggttgc gtgggcgtac cgtttgagag agcgtatgaa aaagaaactg
     1621 ctttcgggca tcaaatttgc cgttcagacg gcattggtgt ttttattggt gtcgctgttt
     1681 ttggattgga tacgcaagcc cgacgaacct gccggtgcgg cagggcggcc tttgaccctg
     1741 ctgtcggggc agcggctgac tttgggacag tttagccggg ataaggcggt tttggtgtat
     1801 ttttggggga gctggtgcgg cgtgtgccgt tatcagtcgc cgataatcga tgatttggcg
     1861 gcggacggcg ttccggtcgt cggcgtggcg gtacgttccg gcagcgcgtc cgaagtggcg
     1921 gcgtatatgg caaagcgggg cttgggcttt ccgacggtca acgatgagga cgggggtttg
     1981 gcaaggtctt ggcggattgt cgcaacgcct gccgtcgttt tggtcaaaaa tgggaaaatg
     2041 gtccattata cgacgggaat cagcagttat tggggcttgc gcgcacgaat ttttcaggcg
     2101 gatgttttcg gttaactttt gtttttgtta aactttacgc tatgtagaga atcaaacggc
     2161 atataccttg ctcgtctgat gcggcggctt ttgttttttc tggaagaaag ttctttcgac
     2221 agtaaatgta atactttgtt gagattgtag ggaaaataga ctaagatata actattaaaa
     2281 tatttttaga taggattatc ggaattaaag tcttttatac ccagtcgtcc gatgcggttt
     2341 atagcgtatt gttgctatat gttcgttttg ttatataacg gttgcatcaa aatttacgcc
     2401 cacaggcttt cccgacggtt tgaaagtttg attttcgata acttggagac ttgaacaatg
     2461 cctacccaat caaaacatgc gtctatcaat atcagtctga tacaggcaag ggaagccctg
     2521 atgacccaat tcaggcctat tctgaatcag gcgaatatta ccgatcagca atggcggatt
     2581 atccgtcttt tggcggaaaa cggcacgctg gactttcaag atttggcgaa tcaggcgtgc
     2641 attctgcgcc ccagcctgac cggtatcctg acccgccttg aaaaagcggg tttggttgtc
     2701 cgcctgaaac cttccaacga ccaacgccgc gtttttctga agctgactgc cgagggcgag
     2761 aagctgtatg aggaaatcgg cgaagaagtg gacgagcgtt acgacgctat cgaggaagtg
     2821 ctgggccgcg agaaaatgct gctgcttaaa gacctgttgg cagaacttgc caaaatcgag
     2881 gatgcgttga actcgtaata cgccgtaacg cgcggaaatg tccgaccgac ggctttttga
     2941 atcagaactg ctgcacatgg ggggtgcctt gtgtgcagca ttcttatata ggggacagtt
     3001 taaaggggaa aaatggcgga tttgcagaaa acttttcaaa cttcgttccg tgatgcgatg
     3061 gcatcctgtg cggcaggcgt tcatgtcatc acgacagacg gtgcggcagg gcgttacggc
     3121 attacaatga cggcggttgc gccggtaacg gacgagccgc cgaccgtgat gctgtgcatc
     3181 aaccggagtg cgcgaatcat tccgatcctg tcggaaaacg gcagcctctg catcaatatg
     3241 ctggcggacg aacatcagga tgttgccgaa cattttgccg ggctgaccgg cctgtcgccc
     3301 gaagagcggt ttgcctacca catctggcat cgcggcaaaa cgggacaacc tgaaatagaa
     3361 ggcgcgttgg cgcacctgca cgggcatatt gtcggcaaac atgaaatcgg cacgcatttt
     3421 gtgttttacg tcaggctcga cgaaatcaaa aactgcgggt gcaaacgccc cgcgctgctg
     3481 tatttcagac ggcagtttag atttttagac tgatattcgg acagatatat gaaagcgatg
     3541 atactggcgg caggacgcgg cgagcgtatg cgccctttga ccgataccac tccgaagccg
     3601 ctgctcgatg tggcgggtaa gcctctaatc ggttggcacc tatgccgtct gaagcaggcg
     3661 gggtttaccg aaatcgtcat caaccacgct tggctgggtc ggcagataga agatgctttg
     3721 ggcgacggct cggcttatgg cgtgaacatc gcctattcgc ccgaacccgc aggcggcttg
     3781 gaaacggcag gcggcatcgc gcaggcattg ccgctgttgg gtgggcagcc gtttttggtg
     3841 gtcaacggcg acgtgctgac cgacatcgat tttaccgccg cgtttcagac ggcatcgtcc
     3901 ctgcccgagc atatttccgc ccatctgtgg ctggtggaaa atccgccgca caaccccgac
     3961 ggcgattttt ccctgctgcc cgacggcagc gtgcggccgg aagtaaatgg cggcaacgga
     4021 ttgacattca gcggcgtggg tatttaccgt cctgaaatgt ttgacggaat cgaagcgggc
     4081 agtgtggcga aactcgcgcc cgtattgcgg aacgaaatgc ggcaaaaccg cgtgagcgga
     4141 cagaagcata cgggcttgtg gctggatgtc ggcacggtat gccgtctgaa agaggctcaa
     4201 gcccttgcag gggcttggaa gtgaaataaa acggtggctt gccgagtcgg ctgccgaacg
     4261 gttccgaccg gatgtccggc atattttcgt ggaggcgtga aaatagggcg ttcggggctt
     4321 tccgtccaac aaatgccatc tgaagcgcgg cggtttgccg tcggcgcggt gtcggatttt
     4381 attgggtaac tttgttcaga ccgccgcccg tcagattgtc aaaacccggt ttcaggcggt
     4441 atggcggatt cggtttaacg tttcaacgcc agcaccaaca cgcccgccgt taccagtccc
     4501 aagcctatcc attcctgcgt gttcgggcgt tcgtccaaaa agaccaccgc catcagggcg
     4561 accaggacca ggctgaattt gtcgacgggc gcgacttgcg aggcgttgcc cagttgcagg
     4621 gctttgaagt aggcgagcca ggatgcgccg gtcgccaaac ccgacagaat taagaatgtc
     4681 cagttttttc ctgtaaatcc gttcacgccc tgccatttgc cggtgtaggt caaaaacagt
     4741 accaaagcgg cgaggatcac caaggtgcgg ataaaggtgg cgaaatccga atcgatgccc
     4801 tgcaaaccca ttttggcgaa aatggcggtc agcgaggcga agcctgccga tgccaatgcc
     4861 caaaacagcc atgcgttgct gcccatgttt tctccttgga ttgtgaacaa tatgaacggt
     4921 atttttgttg cagcgtcaaa aatttcactg cgggtttggt gcggataacg ttataatatg
     4981 cctgatatta ttttcaatcc acctgtttgt cgcctgatgc tttcagacgg tatgtccctc
     5041 ctcatttcta aaggaaaatc atgagcttca aaaccgatgc cgaaatcgcc cagtcttcca
     5101 ccatgcgccc gattggcgaa attgccgcca agctgggcct gaacgccgac aacattgagc
     5161 cttacggtca ttacaaagcc aaaatcaatc ctgccgaagc gttcaaactg ccgcgaaaac
     5221 aaggcaggct gattttggtt accgccatta atccgactcc tgccggcgaa ggtaaaacca
     5281 ccgtaaccat cggtttggcg gacgcattgc gccatatcgg caaagactct gtgattgctt
     5341 tgcgcgagcc ttctttgggt ccggtgttcg gcgtgaaagg cggcgcggca ggcggcggct
     5401 atgcccaagt tttgccgatg gaagacatta atctgcactt taccggcgac tttcacgcca
     5461 tcggcgcggc gaataacctg ctcgccgcta tgctcgacaa ccatatctac caaggcaacg
     5521 ggttgaatat cgatccgaaa cgcgtgctgt ggcggcgcgt ggtcgatatg aacgaccgcc
     5581 agttgcgtca tatcatcgac ggtatgggca aacctgttga cggcgtgatg cgccccgacg
     5641 gcttcgacat taccgttgcc tccgaagtga tggcggtatt ctgtcttgcc aaagacatca
     5701 gcgatttgaa agagcgtttg ggcaacatcc ttgtcgccta cgccaaagac ggcagccccg
     5761 tttacgccaa agatttgaaa gcgaatggcg cgatggcggc attgcttaaa gatgcgatta
     5821 agcccaactt ggtgcaaacc atcgaaggca cgcccgcctt cgtacacggc ggcccgttcg
     5881 ccaacattgc ccacggctgt aactctgtaa cggccacccg tttggcgaaa caccttgccg
     5941 attatgccgt aaccgaagca ggcttcggtg cggatttggg tgcggaaaaa ttctgcgaca
     6001 tcaaatgccg tctggcagat ttgaaacctg atgcagctgt tgtcgtagcc actgtccgcg
     6061 ctttgaaata taacggcggc gtggagcgtg ccaacctcgg cgaagaaaat ttagacgctt
     6121 tggaaaaagg tttgcccaac ctgctgaaac acatttccaa cctgaaaaac gtattcggac
     6181 tgcccgtcgt cgttgcgctc aaccgcttcg tgtccgactc cgatgccgag ttggcgatga
     6241 ttgaaaaagc ctgtgccgaa cacggcgttg aagtttccct gaccgaagtg tggggcaaag
     6301 gcggcgcggg cggcgcggat ttggcgcgca aagtcgtcaa cgccattgaa agtcaaacca
     6361 ataacttcgg tttcgcctac gatgtcgagt tgggcatcaa agacaaaatc cgtgcgattg
     6421 cccaaaaagt gtacggcgcg gaagatgttg atttcagcgc ggaagcgtct gccgaaatcg
     6481 cctcgctgga aaaactgggc ttggacaaaa tgccgatttg catggcgaaa acccaatatt
     6541 ctttgagcga caacgccaaa ctcttgggct gccctgaagg cttccgcatt accgtgcgcg
     6601 gcattaccgt ttcttccggt gctggtttca tcgttgcgtt gtgcggcaat atgatgaaaa
     6661 tgccgggtct gcccaaagtt cccgccgccg agaaaatcga cgtggacgca gaaggcgtga
     6721 ttcacggctt gttctgaacg gtttttgaaa ccagatgccg tctgaagccg tttcagacgg
     6781 cattttttcg gaacgcgggc ggcggtatgc tataatccgc cgttaaattt ctctattttc
     6841 aggaaaaaac atgagtttga aatgcggcat cgtcggtttg cccaacgtcg gcaaatccac
     6901 cctttttaac gcgctgaccc aatcgggtat cgaagcggca aactatcctt tctgtaccat
     6961 cgaacccaac gtcggcatcg tcgaagtccc cgatccgcgt atggccgaat tggcaaaaat
     7021 cgtcaatccg caaaaaatgc agcctgccat cgtcgaattt gtcgatattg ccggtttggt
     7081 tgcaggcgcg agcaaaggcg agggcttggg caaccagttc cttgccaaca tccgcgaaac
     7141 cgatgcgatt gtgaatgtcg tgcgctgctt tgacgacgac aacatcgtcc acgttgcagg
     7201 ccgcgtcgat ccgattgccg acatcgaaac catcggcaca gagttggcac ttgccgacct
     7261 ggcaagtgtg gaaaaagcca tcgtccgcga agaaaaacgc gcccgttcag gcgacaaaga
     7321 cgcgcaaaag ctggtcgatt tgtgcaaaaa actgctgccg catctggacg aaggcaaacc
     7381 cgtgcgttcc ttcggtttgg acgcggaaga acgcgcgatg ctcaaaccgc tgttcctgct
     7441 gacagccaaa cctgcgatgt atgtgggcaa cgtcgccgaa gacggttttg aaaacaatcc
     7501 gcacctcgac cgcctgaaag aattggcggc aaaagaaaac gcccccgtcg tcgccgtatg
     7561 cgccgcgatg gaaagcgaaa tcgccgaatt ggaagacgac gaaaaagccg aattcctcgc
     7621 cgaaatgggt ttggaagaac ccggcctgaa ccgcctgatc cgcgccggct acgacctctt
     7681 gggcctgcaa acctacttca ccgccggcgt gaaagaagtc cgcgcgtgga cgatacacaa
     7741 aggcgacacc gccccgcaag ccgccggcgt gattcatacg gattttgaac gcggcttcat
     7801 ccgcgcccaa gtcatttctt acgacgactt tgtctcgctc ggcggcgaag ccaaagccaa
     7861 agaagccggc aaaatgcgtg tggaaggcaa ggaatacgtc gtgcaggatg gggatgtgat
     7921 gcactttttg tttaacgtgt aacccaaatg cggcaggttt tcagacggct ttgccagaaa
     7981 tgccgtctga agccggtttt ggtggttttc ggcgtttcca taccgccgga atgcggctgc
     8041 atcaaaataa aatcccaccc gcatttccga tttgccctcc ccgattcctg caaaacaaac
     8101 cgcctgcccc gctgttgcaa aaagccgtcc ggcattccga atatcccgta caaaatgacc
     8161 tttcagacgg catttgcgcc gccgcgtttc gagtatagtg gattaactt
//
LOCUS       DAXHKX010000082         8203 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHKX010000082 DAXHKX010000000
VERSION     DAXHKX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.082"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..130
                     /locus_tag="ACFMWZ_001324"
     CDS             <1..130
                     /locus_tag="ACFMWZ_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC5935281.1"
                     /translation="RKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            complement(327..3164)
                     /locus_tag="ACFMWZ_001325"
     CDS             complement(327..3164)
                     /locus_tag="ACFMWZ_001325"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC5935282.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLF
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNTAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(3261..4070)
                     /gene="zupT"
                     /locus_tag="ACFMWZ_001326"
     CDS             complement(3261..4070)
                     /gene="zupT"
                     /locus_tag="ACFMWZ_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC5935283.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFRF"
     gene            complement(4251..5507)
                     /locus_tag="ACFMWZ_001327"
     CDS             complement(4251..5507)
                     /locus_tag="ACFMWZ_001327"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC5935284.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(5586..6977)
                     /locus_tag="ACFMWZ_001328"
     CDS             complement(5586..6977)
                     /locus_tag="ACFMWZ_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC5935285.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(7334..8110)
                     /gene="lpxA"
                     /locus_tag="ACFMWZ_001329"
     CDS             complement(7334..8110)
                     /gene="lpxA"
                     /locus_tag="ACFMWZ_001329"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC5935286.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
BASE COUNT         1840 a         2136 c         2265 g         1962 t
ORIGIN      
        1 ccgtaaattc cgctacgctc gggcagccta tttcggactg attaaagtga gtgcgcaaag
       61 ccacctgaag gcgatgtgtt tgaacctgtt gaaagccgcc aacaggctaa gtgcgcccgc
      121 tgccgcctaa aaagcagccc ggatgcctga ttatcgggta tccgggcagg attaagggga
      181 tatttgggta aaattaggag gtatttggta cgaaaacagc cgaaatcctg tgtttgggtt
      241 tcggctgtcg ggagggacag gaattttgca aaggtctcag gccgtctgaa aaacattttt
      301 cagacggcct tgttgattca atcaaatcag tctttcaact tcgacaactg agtttgcact
      361 tttgccattt tgtcttccaa ttccgccaaa tcggctttgt ctttttccac caaatgcgct
      421 ggggcttttt cggtgtagcc gggtttggag agtttggcgt tgagtttgtc caaggctttt
      481 tgcagcttct cggcttcttt gctcaaacgg gcggtttcgg cagctttgtc gatttcgact
      541 ttcaacatca ggcgcgcgcc gttgcagacg gcgacgggcg cgtcttcgct ttcgggtagg
      601 gcggcgactt gctgtgcttc ggtcaggcgg gtcatcatcg gcaggtattt gaggtagtcc
      661 gccaagtcgt ctgcgctttc gacaaacagc ggggctttca cgttgggctg gatgcccatt
      721 tcgccgcgca ggttgcggac tgcgccgatc aaatcctgca acacggtcat ttgcccgaat
      781 gccgtctgaa caatatcgcc gccgtcggct tcggggaagc gggcgagcat aatgctgtcg
      841 gcggttttgg catcgcacat aggggcgacg gtttgccaca attcttcggt gatgaacggg
      901 ataatcggat gcagcaggcg cagggcggct tcgagtacgc gcaataaggt atggcgtgtg
      961 gcgcgttggc ggctggcgca gccggtttga agctgcactt tggcaagttc caaataccag
     1021 tcgcaatagt cgttccatac gaagctgtac agggtttccg ccgccaaatc aaagcggtag
     1081 gtttcgtagg cttgcgtaac ctgttcgatg gtctgattca gacggcctac aatccacata
     1141 tcggggaagg agtagccgcg cggttcggtg gcggttgcgc cgtagccgca gtcttggttt
     1201 tcggtgttca tcagcacaaa attggtggcg ttccagattt tgttgcagaa gttgcggtag
     1261 ccttcggcgc gtttgaagtc gaagttgacg ctgcgtccca agctggcgta gctcgccata
     1321 gtgaagcgca atgcgtctgc gcccatactc ggaatgcctt cggggaagag ttttttggtg
     1381 gcttcttcca ctttcggcgc ggtttctggt ttgcgcagac cggtcgtgcg ttttaccagc
     1441 agtttgtcca agtcgatgcc gtcgatcaaa tccacagggt cgatgacgtt gccttcggat
     1501 ttggacattt ttttgccttc gtggtcgcgc acgatgccgt ggatgtacac gtctttaaac
     1561 ggtactttgc cggtgaagtg ggtggtcatc ataatcatgc gcgccaccca gaagaagatg
     1621 atttcgtagc cggttaccaa gacgttggac ggcaggaagg ccttgagttc gtcggtttca
     1681 gacggccagc cgagcgtgga gaacggcacg agcgcggagg agaaccatgt gtccaatacg
     1741 tcttcttcgc gggtcaaacc ggttttgccg gcttgttttt cggcttccgc ctgattgcgg
     1801 gcaacgtaca cattgcctgc ctcgtcgtac cacgcaggga tttgatggcc ccaccacagt
     1861 tggcgcgaga tacaccagtc ttggatattg ttcatccatt ggttgtaagt gttgacccag
     1921 ttttcgggga tgaagcgtac cgcgccgctg tcgacggctt ttttggcttt gtcggcgagg
     1981 ctcaagcctt tgaattcgct gtccggctcg ccgccgttcg gtgtggcgga catggcgaca
     2041 aaccattggc tggtcagcat cggttcaatc accgaacctg tgcggtcgcc tttgggcgtc
     2101 atcagcgtgt gcgctttgat ttcgaccaac aagccttgtt cctgcaaatc ggcaaccatt
     2161 tgtttgcgcg cggcaaagcg gtccaaaccc gcgtattttt caggcagggc aaagccttgt
     2221 tgcgcctcgc ctttgaagtt gaacacttcg gcgtttgcca gcactttggc ttccaaatca
     2281 aacacattaa tcaggcgcgt gtcgtggcgt ttgccgactt cgtagtcgtt gaagtcgtgc
     2341 gcgggcgtga ttttcacgca gcctgtgccg aagtcttttt caacgtattc gtcggcaatc
     2401 acgggaatgg tgcggccggt cagcggcagg attaattcct tgccaatcaa atgggtataa
     2461 cgttcgtctt caggattgac ggcaacggca acgtcgccca gcagcgtttc gggacgggtg
     2521 gtcgccacga tgacggcttc ggcggtattg tccgccagcg gatagcggat gtgccacata
     2581 gagccttgtt cttccacgct ttccacttcc aaatccgata ccgccgtacc caaaaccgga
     2641 tcccagttca ccaagcgttt gccacggtaa atcaagcctt gctcaaacag gcgcacgaac
     2701 acttcggtta cggtttcggc gcgcacgtcg tccatcgtga aatactcgcg cgtccagtcg
     2761 gcagagcagc ccacgcggcg catctgctgg gtaatcgtac cgccggaaac ttctttccat
     2821 tcccacactt tttccaagaa tttttcgcgg cccaagtcat gacgcgacac gttttgcgcg
     2881 gcaagctgac gctcgaccac gatttgcgtg gcgatgcctg cgtggtcggt gccgggaatc
     2941 caggcggtgt tgcagccttt catgcggtag tagcgggtca gaccgtccat aatggtttgg
     3001 ttgaaggcat gacccatgtg cagcgtgccg gttacgttgg gcggcggcag ttggatggag
     3061 aaagacggtt tcgtcaaatc catatcaggc tggaaatagc cctgctcttc ccagttttga
     3121 taatgttttg attcgatttc ggctggattg tatttgtcta acatgatgga actttgtgaa
     3181 attaaggtta tttttgatgt gcggattata acgcaaaaag gccgtctgaa tcatttcaga
     3241 cggcctttga catacgggtt ttaaaaacgg aacaatacca ggctgacggc aatcaccgcc
     3301 atacccgttg tcaggccgta aacggtttcg tggccgtctg aatagcgttt ggcggccggc
     3361 agcagttcgt ccaacgccaa aaacaccatc acaccggcta tcacgccgaa taccgaacca
     3421 aacacggcag gcgacaaaaa cggctgcaaa accaaatagc ccaaagccgc tcccaaaggt
     3481 tcggccaagc cggatagtag acacgcccat accgttttct tacggctgcg ggtggcaaaa
     3541 taaaccggcg cggcgatgga aatgccctcc ggaatattat ggatggcaat cgccaaggcc
     3601 aaaggcatcc cgaccgccgg attttccaag gtggcaaaaa atgtcgccaa gccttcggga
     3661 aagttgtgcg ccgtaatcgc aaacgccgcc atcatgccga ctcgcgcgat atggcggcgt
     3721 ttgctttctt gaaacgacgg atcttgcgcg tccaaggttt catgcgggtt cggcaccagg
     3781 cggtcaatca gcgcaatgcc gcccataccg gccaaaaatg ccatggtcgc cgccgcaaac
     3841 gcgtggtctt tatcataaat ttcagcgaac gcctcgctgg atttactgaa aatctccgtc
     3901 agagaaacat acaccatcgc accgccggca aatgccaaac caaacgacaa cacgcgcgga
     3961 ttaggcgttt tggaaaacat taccaaaccg ctgcctaata cggtaaacaa accggcagcc
     4021 aacgtgatgg aaaaggcaac ggccaaattg gacatcgaaa aatcgggcat gagaaaacct
     4081 gcgctaaaag ctgggagagg ttcagactaa cactttttaa tgcgcatgat aatagttatt
     4141 atttatttta ttgattggat acacggattt tgaaacaaaa ggccgtctga aaaataattt
     4201 tcagacggcc tttaaatttg aaatgtcact aaaccttatc gctttccagc ttaagcctga
     4261 taacgcgaca ggctcaaatc gtcgctgcgg atttcggtgt ctttgccgct cacgatatcg
     4321 gcggttaatt ttgccgaacc cagcgacatg gtccagccca aagtaccgtg gccggtattc
     4381 agaaacaggt tgtcaaagcg ggtgcgaccg attaacggcg tgctgtcggg cgtcatcggc
     4441 ctgaggccgc tccagaacga tgcttggctc aaatcgccgc cttccgggaa caagtcgttg
     4501 acgaccaaag ccaaggtttc gcggcgtttt tcgggcagtt tgatttcgta gcccgacaat
     4561 tccgccatac cgccgacgcg gattctgttg tcaaagcgcg tgatggcgac tttgtagctt
     4621 tcatctaaaa cggtggacac cggcgcgccg tctgaattgg tgaccggcag ggtcaaggaa
     4681 tagcctttga cgggataaat cggcagatcg aggtctaact gcgccaacac agtcctactg
     4741 aagcagccga gcgcacagac aacggcatct gcttcaaacc gccctgtttc ggtttcaacg
     4801 gctttgatgc gcagcccgtt atggtcgatg cggctgatgg tttggttgaa atgaaactgt
     4861 acgccctttt cctgacacaa tttgtatagg ttttcagtga agaggtggca gtcgccggtc
     4921 gcatctgcag gcaggtgcag gccgccggca attttggcgg taacgcgtgc cagcgcaggc
     4981 tcaaattctg cacattcttc gggtttcaga cggcggtacg gcacgccgta gcgttccaaa
     5041 acggcaatgt cttgttctgc cgcttcgact tctttggttt ggcggaaaat ctgcaacgtc
     5101 ccttttttgc gttcctcaaa attcatgccg gtttgcgctt caaaacggcg gaacatttca
     5161 cggctatatt cggaaatcct gaccatgcgc tctttattgg tttgatagtg cgctgccgtg
     5221 cagttttgca gcatttgcca cagccattcg atttgataca ggctgccgtc ggggcgaaac
     5281 agcaaaggcg gatggctttt aaacagccat ttcagcgctt tggtcgggat accgggtgca
     5341 gcccaaggcg tggtatagcc gtaagaaagc tggcctgcgt tggcaaaact ggtttccatc
     5401 gccacaccct cggcgcggtc gatgaccgtt acttcatgtc ctgcctctgc cagataccac
     5461 gcggaagaca cgccggcaac acccgcacct aaaacaagca ctttcatgtt tctccctccg
     5521 gctttttcaa aacagactta atatgccgtg ccgtctgaat attcggattc agacggcctc
     5581 ggatattaat gcggcaattc gccgtttgtg attttttgtt tgaagtcgcg cgtttcattg
     5641 acgatgactt tcgccatcaa taaaagtgca atcaggttgg gcaatgccat caagccgttg
     5701 aatgtgtccg aagccagcca caccaaatca aggctcaaca cggtacccag cataacggaa
     5761 gaaacataac cgacgcggta caaaccggca aatttctcgc cgaaaacata caccgcgcat
     5821 ttttcgccgt aatagcacca gccgagaatg gttgagtagg caaagaaaat caggccgatg
     5881 gtgacaatcc agccgccgat gccgggcagc attttttgga atgtgacggt tgtcagtgcc
     5941 gcgccgctca cttcaggttt gacaaactcg ccgcccgcgc cgagcagtcc cattaccaac
     6001 acgatgccgg taatcgagca aacgacgatg gtatccaaaa acgtaccggt catagaaacc
     6061 aaggcctgac ggacgggatg gtcggttttc gcggctgcgg cggcaatagg cgcagaaccc
     6121 atacccgcct cattggagaa cacgccgcgc gccacgccgt agcggatgac cgtaccgata
     6181 gcaccgcccg ccactgcctg cgcgctgaac gcatcggaga aaatcagctt gacggcaggc
     6241 atcagtgcat cggaattaat cgcgataatg gaaagaccgc ccaacacata aaacaccgcc
     6301 atagcaggca cgatgaaaga agcggctttg gcgatgcctt taataccacc taaaacgaca
     6361 acggcagtca gaacggtcaa cgtaatgccg gtataggcag gttcgatacc gaagctggtt
     6421 tgcaccgcct gtgcaaccga gttggactgc accgagctgc cgataccgaa ggaagcgaat
     6481 gtgccgaaca gcgcaaatgc aacggccatc catttccagt ttttgcccaa gcctttttcg
     6541 atgtaataca tcgggccgcc ggacatttcg cctttggaat tgttgacgcg gtatttcacc
     6601 gccaacacgc cttcgccgta tttggtggcc atgccgaaaa tggcggtcat ccacatccaa
     6661 aataccgcgc ccgggccgcc ggttaccacc gcagtcgcca cgccggcgat gttacccgta
     6721 ccgatggtgg cggacagcgc ggtcatcaac gccgcgaaat gggaaatatc gccttcgtgg
     6781 ccttcgccgc cttcatactt ctttgacggc acaaacgcct gtttcagcgc atagcccaac
     6841 atagtgaact gcaagccttt cagcataacg gtcagcacaa tgccggtgcc gaccagcagc
     6901 atcaacataa cagatcccca gacccagccg ctgacggttt cgaaaaaggc tttgagattg
     6961 tctaaaaaca attgcatggc tttctccttt gtctgtttta tttttaaaac accacttttg
     7021 tagtgtcctg taatttcagc acagaatatt caataagaca atatgttctt ttgaaaaata
     7081 cttttggttt tttctccgaa agcaggtcgg ttcaagctgc ggaaattgtt tgcaattctt
     7141 taaaagcagc ggcggaggtc acaatgaaat gtccgaatgg ggatgtggcg ggcggcagaa
     7201 atcatcaatg ctgccgactg ccatacttct gaaatctaca aaatgatgca tcgatcaaac
     7261 aatataccgc tttaaaaaaa ccgatgccgt ctgaaacgct ttcggggttt cagacggcat
     7321 caaaagggta cggtcagcgg atgatgccgc gcgccgattg tgcgaaaaag tctcggaata
     7381 cggcaagctc ggcttgggtt tcggcgcggc ggagaatgtc tgccttggct tcttcaaacg
     7441 gaatgccgcg atggtagagg gttttgtaca cgtctttgac ggcggaaatc tgctctgcgg
     7501 taaaaccgtt gcggcgcatg ccttcgctgt tgagccccgc cggttcggcg cggtagcccg
     7561 atgccataaa gtagggcggt acgtctttgt gtacgcctgc ggcgaaggcg gtcatagcgt
     7621 agtcgccgat gtggcagaat tggaaaacca gcgtgtagcc gcccaaaacg acgtagtcgc
     7681 cgatggtaac gtgtccggca agcgaggcgt tgttggcgaa gatggtgtgg ttgccgacca
     7741 cgcagtcgtg cgcgaggtgg cagtacgcca taatccagtt gtcgtcgccg atacgggttt
     7801 cgccgatgcc ggttaccgtg cctaaattaa aggtggtgaa ttcgcggatg gtgttgccgt
     7861 tgccgataat cagcttggtc ggctcgtcgc ggtatttttt gtcctgcggg atttcgccga
     7921 ggctggcaaa ttggaaaatg cggttgtttt cgccgatgct ggtgtggccg ttgatgacgg
     7981 cgtgcgggcc gatttcggta ttcgcgccga tttggacgtt ggggccgatg acggtgtacg
     8041 cgccgacttt gacgctggag tcgagttcgg ctttggggtc gatgacggcg gtcgggtgga
     8101 tgagggtcat gttttgaata ctccgctgct tgttaagaca aataatagaa aatcacagtt
     8161 tgtcgatttc atcgcagacc gcaatcactt gatcgctaaa atg
//
LOCUS       DAXHKX010000083         8134 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHKX010000083 DAXHKX010000000
VERSION     DAXHKX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.083"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..204
                     /locus_tag="ACFMWZ_001330"
     CDS             <1..204
                     /locus_tag="ACFMWZ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935287.1"
                     /translation="IHYKIPRVRHAPRSAMIGAVSFESAYRDECPRAVPIPICRLFRI
                     GCRREMTDGCRNGGRREWEEMTD"
     gene            309..1622
                     /locus_tag="ACFMWZ_001331"
     CDS             309..1622
                     /locus_tag="ACFMWZ_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC5935288.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKKRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1840..2021
                     /locus_tag="ACFMWZ_001332"
                     /pseudo
     CDS             1840..2021
                     /locus_tag="ACFMWZ_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2623..3321
                     /locus_tag="ACFMWZ_001333"
     CDS             2623..3321
                     /locus_tag="ACFMWZ_001333"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFC5935289.1"
                     /translation="MTALPLQAERLNNMPLPPDAARQPAVLQQSIRRNKHLLEQLLAL
                     ARSQSDETPLTKTTFGLQSRFRQVLQELMPLALEKRQDIGVAVGGDVEVSADETEIYT
                     LIKTFADNAVRYTPPEGRIDLRFTDEGKYLAVWVEDNGNGIPESERARVLDPFYRILG
                     TEQQGTGLGAVDCRHACQKIRRTFGIDRQPTFRTRSVDTRVVGQGNPEIDRCRLKASD
                     GIFYISTISYCSCV"
     gene            complement(3285..4778)
                     /gene="rng"
                     /locus_tag="ACFMWZ_001334"
     CDS             complement(3285..4778)
                     /gene="rng"
                     /locus_tag="ACFMWZ_001334"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC5935290.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4991..5248
                     /gene="grxC"
                     /locus_tag="ACFMWZ_001335"
     CDS             4991..5248
                     /gene="grxC"
                     /locus_tag="ACFMWZ_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC5935291.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5269..5712
                     /gene="secB"
                     /locus_tag="ACFMWZ_001336"
     CDS             5269..5712
                     /gene="secB"
                     /locus_tag="ACFMWZ_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC5935292.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5798..7840
                     /gene="recG"
                     /locus_tag="ACFMWZ_001337"
     CDS             5798..7840
                     /gene="recG"
                     /locus_tag="ACFMWZ_001337"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC5935293.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTATETLEQLQTALPELNIG
                     LVHGRMKAAEKAEVMAQFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1729 a         2123 c         2376 g         1906 t
ORIGIN      
        1 atccactata aaatcccgcg tgtacggcac gccccgcgct ccgcaatgat aggggcggtt
       61 tcgtttgaat cggcgtaccg ggatgaatgc ccccgcgccg tcccgatacc gatttgccgg
      121 ttgttccgta tcggttgccg ccgtgaaatg acggacggat gcaggaatgg cggcaggcgt
      181 gaatgggagg aaatgaccga ttgacttgtt tcaaaataaa aaagtagaat gcaaggctta
      241 tcccgcgtct gtgggcggtt ttgcctgcac ggtcggtttt ctgtttaaaa taaaataaag
      301 gagaaatgat gctgacgttt atcggactgc tgattatcgg ggtcatcgta tggctgctgc
      361 tgacggaaaa agtgtcgccc atcatcgcat taatcttggt gccgctgttt ggggcgttgc
      421 tggcggggtt tgatgtatcc caattaaaag aattttattc gggcggcacc aaatcggtga
      481 tgcagattgt gattatgttt atgttttcca ttttgttttt tggaatcatg aacgatgtgg
      541 ggctgttccg tccgatgata ggcggtttga ttaagctgac tcggggtaat atcgtggcag
      601 tgagtgtggg gacggtcttg gtgtcggtgg tggcgcagtt ggacggggcg ggtgcgacga
      661 cgtttttatt ggtcgtcccc gcccttttgc cgctttacaa gcgtctgcat atgaatcctt
      721 acctgctgtt tttgctgcta acttccagtg cgggattgat taaccttctg ccgtggggcg
      781 ggccgactgg gcgggttgca agcgtgttgg gcgcagatgt gggcgaattg tataaacctt
      841 tgttgacggt gcaaattatc ggtgtagtgt ttatccttgc gctgtccctg cttttgggtg
      901 tgcgtgaaaa aaagcggatt gtccgggagt tgggcgcgtt gcccgccgtg gcggatttga
      961 taaagccggt gcctttgtcg gaagaagaac aaaaattggc gcgtccgaaa ctgttttggt
     1021 ggaatgtcct gctgtttttg gcggcgatga gcctgctttt ttcgggcatc ttcccgccgg
     1081 gttatgtatt tatgctggct gcaacggcgg cgttgctttt gaattaccgc agcccgcagg
     1141 aacagatgga gcggatttat gcccacgccg gcggcgcggt gatgatggcg tccattattt
     1201 tggcggcagg tacgtttttg gggattttga agggtgcggg gatgttggac gcgatttcca
     1261 aagacattgt gcatatcctg ccggacgcgc tgctgcctta tctgcatatt gccatcggtg
     1321 tgttgggcat tccgcttgag ttggttttga gtacggacgc ttattatttc ggactgtttc
     1381 cgattgtgga gcagattacc tcgcaggcgg gcgtggcgcc cgaagcagca ggttatgcga
     1441 tgttgatcgg cagtatcgtc ggcacttttg ttacgccgct ttcgccggct ttgtggatgg
     1501 gcttgggttt ggcgaaattg tcgatgggca aacacatccg ttattcgttt ttctgggcgt
     1561 ggggtttgtc gctggcgata ttggccagtt cgatagcggc aggaatcgtg cctctgccgt
     1621 aaacggcgaa gccgtctgaa gcccgaatcg ttcctgtggt ctgtcgcagg ctctgcctgc
     1681 ccgtgtgttg caatcgcgcc ccaatgccgt ctgaaggcag ttgccggcgc ggttttttgt
     1741 gccgcagttt tgaaaaatac cggcaagcgt aaaggccgct aaattgcggc taattttggg
     1801 gtgttagaat aggcacggtt tattttgaag ggaaagttga tgcgtgtttt gctggtggaa
     1861 gacgatgcga tgattgcgga ggcggtgtcg gcaagtttga aagacggcgg ctatgcggtg
     1921 gattgggtca aaaacggcgc gcggctgccg ctgcccagct ttatgacttg atgctgctgg
     1981 atttgggttt gctggggcgg gacggtttgg atgtttgttc cgctggcaat gcttgcaggt
     2041 atgttttcct actacgaaac cttccacgaa acggaagcgt tgcaggacga cctgctccgt
     2101 caggcggcat tgtacgtcgc acccgattcc aaacccgaaa ctttgcccga gggcgacggc
     2161 gatacgcgca ttttcgtgca gatgccgcag caggaagacc ctgttgtcag cctgcctgcg
     2221 catctggcgg acggtctgca cgacggggac gacgattatt accgcgtcta tatccgcacg
     2281 accgaacagg gacggattgc cgtcatgcag gaaaacgaat accgtgaaga tttggcggcg
     2341 gatgcggcac ggcaaagcgt gttgcccctg ttggcggcac tgccgctgat gattttgctg
     2401 accgtgtgga ttacgcacaa agccatgcgc cccgtccgca aattgtcgca aagtctcgaa
     2461 caacgccgaa tcaatgacct gtctgctttg agtgtggaca atattcccag tgaaatcaga
     2521 gctttcgtaa ccgccatcaa cctgcttttg aaacgtgcgg atgaagacat ccgccaccgt
     2581 cagcggtttg tcgccgacgc ggcacacgaa ttgcgtacgc cgatgaccgc ccttcccctt
     2641 caggcggaac ggctcaacaa tatgccgctc ccacccgatg cggcgcggca gcctgccgtt
     2701 ttgcagcaaa gcatacggcg taacaaacac ctgctcgaac agcttttggc attggcgcgt
     2761 tcgcagtcgg atgaaacccc tctgacgaaa acgacattcg gtctgcaaag ccgtttccgc
     2821 caagtgttgc aggaactgat gccgctggct ttggaaaaac gtcaggacat cggcgtggcg
     2881 gtcggaggcg atgtcgaagt gtctgccgac gaaacggaaa tctatacgct gattaaaacc
     2941 tttgccgaca acgcggtacg ttacacgccg cccgaaggca ggatagattt gcgtttcacg
     3001 gacgaaggga aatatctcgc cgtgtgggtg gaagataacg ggaacggcat tcccgaatcc
     3061 gaacgcgccc gcgtcctcga tccgttttac cgtattttgg gaacggagca gcaggggacg
     3121 gggctggggg ctgtcgattg ccgacacgct tgccaaaaaa tacggcggac atttggaatt
     3181 gaccgacagc cgacgtttcg gacacggtct gttgatacgc gcgttgttgg acaaggaaac
     3241 cctgaaatag acagatgccg tctgaaggct tcagacggca ttttttacat cagcacaata
     3301 tcgtattgtt cctgcgtgta agcggtttcg accgccagag aaatcggttt gccgatgaaa
     3361 tctatcagca ttgccaagga ttgcgattct tcgtccaaaa acaaatcgat gacgttgggg
     3421 gcggcgagga tgcggaaact ttcggcatcg taacggcgcg cttcgcggac gatttcgcgc
     3481 tggatttcgt agcacacggt ttgcggcgtt ttcagacggc ctctgccttg gcaggaaggg
     3541 cagggttcgc agaggacttg gtttaagttt tcgcgcgagc gtttgcgcgt cagctcgacc
     3601 agccccaggc tggtaaaacc gtgcagggta acgcgggtac ggtcgaaggc gagggctttg
     3661 gcaagctcct gcaacacggc ttcgcggtgg ctttcctgcg ccatatcgat gaagtcgatg
     3721 atgatgatgc cgccgaggtt gcgtagcctc aattcgcggg cgatggtgtg gcaggcttcg
     3781 aggttggtgc ggaagatggt ttcgtcgaag ttgcgtgcgc cgacgaagcc gccggtgttc
     3841 acgtctatcg tggtcatcgc ttcggtggat tcgataatca ggtagctgcc gaagttgagg
     3901 ttgacgcgcg gttgcagggc gcggctgatt tcctgttcga cgttgtgggt ttcaaacagc
     3961 gggcgttcgc ctttgaacag ctctatcctg cccaatgcgc cgtggacgta ttgttcggca
     4021 aaacgcgtca tgcgcccgtg gtttacggtg gaatcgacga ggattttctg cgtgtcgcag
     4081 ccgaccatat cgcgcaatac gcgcaaactc aagggcaaat cctgataaag cagggtttcc
     4141 ggcggccgga ttttcgcctg ttcttggatg tgttcccaca ctttggtcag gtagtcgatg
     4201 tcggactgga gctgttcgtc ggtggcgttt tcggcgttgg tgcggatgat gtagccccgg
     4261 caggcatttt ccggcaggag cttgtcgagg cgttcgcgca ggctgctgcg ttcggcatcg
     4321 tcttcgatgc gttgggacac gccgatgtgg tcttcttgcg gaagatggac gaggaaacgc
     4381 cccgccagcg agatttgggt ggaaagccgc gcgcctttgg tgttgatcgg gtctttgatg
     4441 acctgcacca aaacagactg cccttcaaac agcatatgtt cgatgcgctg ggtttcttcg
     4501 gggttgcggc gttgttcgag gacatcgacg atgtgtaaaa acgccgcgcg ttccaagccg
     4561 atgtcgataa acgcgctctg catcccgggc agcacgcggc gcaccacgcc cagatagata
     4621 ttgccgacca ggctgtgttc gctgttgcgc tcgatgtgca gctcgcagat attgttttcc
     4681 tccaacaccg ctacgcgcgt ttcctgcggc gtgatgttga ccaatatcgt ttcgggcggg
     4741 cgcgcgatgt ctttggggat ggggagtcct gacaacatgg tttttcctga aaatgtaata
     4801 aaaatatttt gcatccgttc cgccgcgcag cgcgggtgca aaaatatttt tcacaatgtc
     4861 tatcatactt taaaaaagaa actttgacac attccgcaca tgccgtttga acgtgcatct
     4921 gttccgcact tgccaaacag gggaaatgcc cctatattga ttatcattgc aaaactttcg
     4981 gaaacccaat atgcagaccg ttaccatgta cacaggtccg ttttgcccct actgcgcgat
     5041 ggcgaaaagg ctgctgcacg cggcaggtgt cggacatatc gacgaaatcc gtgtcgatgc
     5101 aagccccgaa gcctttgccg aaatgcagca gctttcggga cagcgcagcg tgccgcagat
     5161 tttcatcggc gaaacgcacg tcggcggatt taccgacctc taccgcctcc agcaggaagg
     5221 cgggctagac ggactgctga acccttaacc cccaactagg aaaacaaaat gagcgaagaa
     5281 ctgcaacccg tattcagcat cgagcgactg tatgtcaaag acttgtcttt ggaagtgccg
     5341 cacgcgccgc aaatcttttt ggaacagggc gagcctgaag tggatatgcg cgtttccacc
     5401 ggcagccaaa agctggaaga cggctactac aacgtggacg ttaccgtaac cgttaccgcc
     5461 aagctggata acgagcgcac gatgtttttg aacgaagtaa cccaaagcgg catcttccgt
     5521 ttggaaaaca tccccgaaga agatgccgac ctgctgctcg gcgtggcgtg tccgaacatc
     5581 ctcttccctt acgcgcgcga agccgtttcc ggtacggtaa cgcgcgccgg cttcccgccc
     5641 gtcctgctcg cgccgattaa ttttgaggcg atttaccaac aacagcagga agccgaagcc
     5701 gccggagctt gattcctgcc tgtcgatgcc gtctgaatcc gtttcagacg gcatttttgt
     5761 tttcggataa aataggcgcg tccaaaggaa tctgcctatg atgtcgcccg aaacccgaaa
     5821 acagctcaaa atcaccgatg tttccgccaa gaagctcgac aaactcaacc tccataccgc
     5881 gtgggatttg gtgttgcacc tgccgctgcg ttacgaggac gagacgcaca ttatgccgat
     5941 taaggacgcg ccgattggcg tgccgtgtca ggtcgagggg gaggttatcc atcaggaagt
     6001 aacgttcaaa ccgcgcaagc agctgattgt ccaaatcgcc gacggctccg gcagcgtcct
     6061 ttttctgcgc ttcatccact tttacgccag ccatcagaaa cagacggcgg tcggcaaacg
     6121 catccgcgcc gtgggcgaaa tcaaacacgg cttttacggc gacgagatga ttcatcccaa
     6181 aatccgcgat gccgagggcg gcggtttggc ggaaagcctc acgccggttt acccgaccgt
     6241 aaacggtttg aaccagccca ctttgcgccg tattattcag acggcgttgg acgttacgcc
     6301 gctgcacgac acgctgcccg atgccctgct gtgccgtctg aagctgccac acctcgccga
     6361 aagcctgcgt cttttgcatt cgccgccgcc gagtttcacg attcatcagc tttcagacgg
     6421 cacgctgcct gcatggcaac ggctcaaatt cgatgaactt ttggcgcaac agctttcgat
     6481 gcgcttggcg cgacagaagc gtatcggcgg cacggcggcg gcattgggcg gcgacggcac
     6541 attgacccaa gccctgcgcc aagccctgcc gtttgccctg accgatgcgc aagaaaaagt
     6601 tgtttccgaa atctgccgcg atatggcgca aacctacccg atgcaccgcc tgctgcaggg
     6661 cgatgtcggc agcggcaaaa ccattgtcgc cgccttgtcc gcgctgaccg ccatcgaatc
     6721 cggtgcgcag gtggctgtaa tggcgcccac tgaaatcctt gccgaacagc attttattaa
     6781 atttaaacaa tggctcgaac ctttgggcat tgaagttgtc cgcctttttg gcagtttgcg
     6841 taaaaaagcc aaagacgaag ccaaagccaa acttgccgac ggcagcgtca aaatcgccgt
     6901 cggcacgcac gccctgtttt cagacggcgt ggcgtttcac aatttgggct tgaccattgt
     6961 ggacgaacag caccgtttcg gcgttgccca acgcctcgcc ctcaaaaaca aagggcgcga
     7021 agtccatcag ctgatgatgt ccgccacgcc catcccgcgc acgcttgcga tgagtttttt
     7081 cgccgatttg gacgtgtccg tcatcgacga attgccgccc gggcgcacac cgattaaaac
     7141 gcgcctcgtc aacaacgtcc gccgcgccga agtcgaaggc ttcgtcctcg gcacttgccg
     7201 aaaagggcgg caggcgtatt gggtctgccc attgattgaa gagagcgaaa ccctgcaact
     7261 gcaaactgct accgaaaccc tagagcagct tcagacggcc ttacccgaac tcaacatcgg
     7321 cttggtacac gggcgcatga aagccgccga aaaagccgaa gtcatggctc agtttgccgc
     7381 aggccgtctg aatgtcttgg tcgccaccac cgtcatcgaa gtcggcgtag atgtgcccaa
     7441 tgccgccctg atggtcatcg aacacgccga gcgcatgggc ttggcgcagc tgcaccaatt
     7501 acgcggacgg gtagggcgcg gcgcggcaga aagcgtgtgc gtcctcctgt ttgccgaacc
     7561 cttgggcgaa ctcgccaaag cgcggctgaa agtgatttac gaacacaccg acggctttga
     7621 aatcgcccgc caagacctca acatccgcgg ccccggcgaa tttctcggcg cgcgccaaag
     7681 cggcgtgcct atgctgcgtt tcgccaagct cgaagaagac ttacaccttt tggaacaagc
     7741 gcgcgaaacc gccccgatgc tgattgaaca aaaccccgaa atcgtcgaag cgcatttggc
     7801 aagatggctt tccggcaggg aaggttattt gggcgtgtga gcaaaatgcc gtctgaaata
     7861 ttggaaaaaa gtgcaacgga atctgatttt ttatagtgga ttaacaaaaa tcaggacaag
     7921 gcgacgaagc cgcagacagt acagatagta cggaaccgat tcacttagtg cttcagcacc
     7981 ttagagaatc gttctctttg agctaaggcg aggtaacgcc gtactggttt ttgttaatcc
     8041 actatattat ttttgacaca ttttcatgat ttcaaatccc gttattccca agtggacgga
     8101 aacccgataa tgggaagccg cagagactta tcgg
//
LOCUS       DAXHKX010000084         8120 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHKX010000084 DAXHKX010000000
VERSION     DAXHKX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.084"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(174..2231)
                     /gene="ppk1"
                     /locus_tag="ACFMWZ_001338"
     CDS             complement(174..2231)
                     /gene="ppk1"
                     /locus_tag="ACFMWZ_001338"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC5935294.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDQNVPLLERLCFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAREGIHFYRRRNWTDTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVDRPDLKFPPHTQGRLKALGKNGSIFK
                     LIRRAPILLHHPYQSFNPVVEMMREAAADPAVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2400..2843)
                     /locus_tag="ACFMWZ_001339"
     CDS             complement(2400..2843)
                     /locus_tag="ACFMWZ_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC5935295.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDTVGDFIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(2850..3365)
                     /locus_tag="ACFMWZ_001340"
     CDS             complement(2850..3365)
                     /locus_tag="ACFMWZ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC5935296.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            3635..6265
                     /gene="leuS"
                     /locus_tag="ACFMWZ_001341"
     CDS             3635..6265
                     /gene="leuS"
                     /locus_tag="ACFMWZ_001341"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC5935297.1"
                     /translation="MQEQYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            6493..7284
                     /locus_tag="ACFMWZ_001342"
     CDS             6493..7284
                     /locus_tag="ACFMWZ_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC5935298.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFEEKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTPKAF"
     gene            7251..7403
                     /locus_tag="ACFMWZ_001343"
                     /pseudo
     CDS             7251..7403
                     /locus_tag="ACFMWZ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            7414..7527
                     /locus_tag="ACFMWZ_001344"
     CDS             7414..7527
                     /locus_tag="ACFMWZ_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HFC5935299.1"
                     /translation="MRDSDKPAASEGAVKFIEGNPAKKIIAVPGRLVNIVV"
     gene            7738..>8120
                     /locus_tag="ACFMWZ_001345"
     CDS             7738..>8120
                     /locus_tag="ACFMWZ_001345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HFC5935300.1"
                     /translation="MNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYAD
                     AANNTGHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTH
                     ALHEHGKNKVGQKILTNMAAQIGTQ"
BASE COUNT         1945 a         2100 c         2264 g         1811 t
ORIGIN      
        1 tgccgtctgg aatgcggcgg attggggttg ggagcgtata ggggaagtgc ttatgcggaa
       61 atggcgaagg tgtgtttttg actccggtcc gccctgttta gattgctgta aaaccatgcc
      121 gtctgaaacg gaaaggtttc agacggcatg gtttttgatt ggggcggtgc gggtcagcct
      181 ccgagcagtg tccacaaatc gttttgcagg tcggcttcgg attcgccctc ggcaggtgcg
      241 gcgcggatat agccgccgtc gggctgcatc agccacgcgt gcgtattgtc tgccaatgcc
      301 atttccaaac cttcgcggat gacgcgtttt ttgagttcgg gcgcggtaat cggcgcggcg
      361 gtttcgatgc ggcggaagaa gttgcgcccc atccaatccg cgctggagat aaaggtatcg
      421 tccgcgccgt tgttgtggaa gcaatacacg cgcgcgtgtt cgagctgcct gccgatgatg
      481 gagcggacgc ggatgttttc ggacaagcct tttacacccg ggcgcaaggt gcacataccg
      541 cgcacaatca aatcgatttg tacgcctgct gcgcttgccc gatacagggc ttcgatgacg
      601 gtcggttcga tgagcgaatt catcttggcg gtaatccgcg ccggtttgcc ggcttttgcg
      661 tgttcggttt cgcgtgcgat gcggtcgata accattttgt gcagggtaaa cgggctttgg
      721 tagagtttgt tcagccgccc gggtttgccc aagcctgtga tttccataaa caatgtgttc
      781 acatcggcgg tgatttgttc gtcggcggta atgatgccga agtcggtgta gatgcgcgat
      841 gtgccttggt ggtagttacc cgtgccgagg tgggcgtaac gcttgagtac gccgtcttcg
      901 cggcggatga ccagtgccat cttggcgtgg actttgtagc cgaacacgcc gtacacgacg
      961 tgcgcgcccg cctcttcgag ctgcttcgcc cagttgacgt tgttggcttc gtcaaaacgc
     1021 gccatcagtt cgacgacgac ggttacttgt ttgccggcga gtgccgcctt catcagggcg
     1081 cggacgagtt cggaacgcgt gccggtgcgg taaatcgtca ttttgacggc aagcacggcg
     1141 gggtcggcgg cggcttcgcg catcatttcg acaacgggat tgaaggattg gtaggggtgg
     1201 tgcagcagga tgggtgcacg gcgtatcagt ttgaatatcg agccgttttt acccaaggct
     1261 ttcaacctgc cttgtgtatg gggcggaaat tttaaatcag gacggtcaac caaatcgggg
     1321 acggcgttga ggcgcacgag gttgaccggg cctttgacct gatagagttc ggcggcggtc
     1381 agtttgaatt gcgcgagcag aaagtcgcgg atgtaggcgg gacaggtgtc ggcgacttcg
     1441 agccgcacgc cgtcgccgta ttcgcggtcg tgcaactcgt tttgaatggc ggcgcggagg
     1501 ttttgtacgt cttcttcgtc aacggtcaag tcgctgtcgc gcgtgaggcg gaactggtgg
     1561 cagcctttga cgttcatgcc cgggaagagt ttgccgacgt gggcgtggag gatggacgac
     1621 aggaagacga agccgtgtcc gccgccacac agttcggacg gcaggggaac aacgcgcggc
     1681 aggatgcgcg gtgcctgcac aatcgccatc cccgaaggcc tgccgaacgc gtccgtgccg
     1741 tcgagttcga cggcgaagtt gagcgatttg ttcagcggtc gcgggaaggg gtgggacggg
     1801 tcaaggccaa tgggggtcag aatcggcaac aactcacggt caaaatagtc ttcaatccat
     1861 tttttctgtg tgtctgtcca attgcggcgg cggtaaaaat ggatgccttc ccgcgccaac
     1921 tcgggctgaa ggacgttgtt gaacaggtcg tactggtgcc gtatcaggga gcgcgccgct
     1981 tcggtaacgt cggcgatggt ttcagacggc attttgccgt tgtccagcct gcgccggggg
     2041 tgcagcttgt tttcacgttt gagccacgcc atgcggactt cgaaaaactc gtcgaggttg
     2101 gatgaaacga tgcacaggaa gcacaggcgt tccaaaaggg ggacgttttg gtcttccgcc
     2161 tgcgccaaca cgcggcggtt gaatgccagc aagctcagtt cgcggcagag gatgcggttt
     2221 tgttcgggca taaggttctc ccaaagatgg ttgttgttcg gtcggagcgg gcaggcaatg
     2281 ccgtctgaag gcttggcggc atctgcgccg gtttcagacg gcatgacgtg gcggacagtc
     2341 tgtttcaggc atcggggcaa tgtgaaaaca gataccggcg cgtgcggtat ccgtctgttt
     2401 caaatcactt ccaacacaaa ataatgacgc agtttttcgt acaccgcatc gctgacgttg
     2461 atgcagccgt tggtcataat cctgtcggac acggacgggg aggcgatgcg ttcgttccgc
     2521 ctttccgacg gtatctgatt ccaaacgcgg tgcagggcga aaagaaaatc accctcctgc
     2581 ttgaaaccga tgacttcgcc gccgtatccg ggcttgtcgg tactgttcag cgtcagcccg
     2641 aacgtcccct tgggcgtggc aacaccgatg aggacggggt ggcactgacg gtcgtcagcg
     2701 aaacagagtt ccgctttgga tgtatcgacg atgacttttt tcttttggat aaagtcgccg
     2761 accgtatccg gctgcccctg tgcatatacg ggggcaacca ttgacaggcc gcataatatc
     2821 cctaaaaaca ggcggaacgg catgtcggat tattggcgga tacgtttcgg ttttgccggc
     2881 acgatttcgc ggatgatgac ttgcggctcg gctttgggtg cgggcggcgg acaggcggca
     2941 tctttcggga agacggggtt ccagtagaag ctgcgggcaa atttgtcttt atcgaaaatc
     3001 actttgtatt ggcaggtagt aacatcttct acgccggaag tgttttcagg gtcgatacct
     3061 acgcccgggg tatggaagtg gaacaggtaa tcccattcgc gcacgccgta cataccttcg
     3121 tcgtaatgcg ggcggcccag gattttgtag atgtcgtctt tggtcaggcc ggggcgcatc
     3181 tgatccagtt cgtcataagt cgggaatgtg ccgcgcttgt tgtcgagcgt tacggaatag
     3241 ggtttcggga aaaccggatt gtcggtcgtg ccgtcggctt tgacgttgct tttggttgcg
     3301 caggcagaca gaacgcccgc cgccaatact gccaagcccg gtttgacgat ttgtttgatt
     3361 ttcatatgca gaatcctctt ttacccgatg cccgtctgcc ttttgttcgg aagagccgca
     3421 tcaggagagt tttaaacatt tgattttggt ttgtaatatt agcataaaaa acgtgcgtat
     3481 caataaacgc ggtgtatttg tacggcatac ggaataatgc gcgtgcgaat ttacgcatcc
     3541 tgccggcaat ctgccgattc gccgcttccg gcaacctgtt ataattgatg ccgactttat
     3601 ttttccaacg tttttaagcg aaaacagaat gactatgcaa gaacaatacc ggcccgccgc
     3661 cattgagcct gcggcgcaga aaaaatggga cgacgcccgt attttcaacg tctccgaaga
     3721 cgcttccaaa cccaaatatt attgcctctc catgttcccc tatcccagcg gcaagctgca
     3781 catggggcat gtgcgcaact acaccatcgg cgatgtattg agccgcttca aacttttaaa
     3841 cggcttcaac gtcatgcagc ctatgggttg ggacgcgttc ggcatgccgg cggaaaacgc
     3901 ggcgatgaaa aacaacgtcg cccctgccgc ttggacgtac gataacatcg aatacatgaa
     3961 aacccagctc aaaagcctag gttttgcgat tgactgggcg cgcgaaaccg caacctgcaa
     4021 acccgaatac taccgctggg aacaatggct gtttaccaag ctgtttgaaa aaggcatcgt
     4081 gtatcgcaaa aacggcacgg tgaactggga ccctgtcgac caaaccgtcc ttgccaacga
     4141 gcaagtcatc gacggacgcg gctggcgttc cggcgcattg atcgaaaaac gcgaaatccc
     4201 gatgtattac ttcaaaatca cggattacgc cgaagagctg ctcaacgact tggacaagct
     4261 ggaacactgg ccggaacaag tcaaaaccat gcagcgcaac tggatcggca aatcgcgcgg
     4321 tatgaccgtg cgcttcgccg tttcagacga cagcaaacaa ggcttggaag gcgattacgc
     4381 gaaattcctg caagtttata ccacccgccc cgacacgctg atgggtgcga cttatgttgc
     4441 cgttgccgcc gagcatccgc tggcagccgc cgcagccgcc gacaaacccg aattgcaggc
     4501 atttattgcc gaatgcaaag ccggttcggt tgccgaagcc gatatggcga cgatggagaa
     4561 aaaaggcgtg ccgaccggtc gctacgtcgt caacccgctc aacggcgaca agctggaagt
     4621 gtggattgcc aactatgtat tgtggggcta cggcgacggc gcagtgatgg cggttccggc
     4681 gcacgacgaa cgcgattttg agttcgccgc caaatacagc ctgccgaaaa aacaggtcat
     4741 tgccgtcggc gacaacgcat ttgacgaaaa ccaatggcaa gaatggtacg gcgacaaaga
     4801 aaacggcgta ttggtcaaca gcggcgactt ggacggcttg gattttcaga cggcattcga
     4861 tgccgttgcc gccaagctgc aaagccaagg cgcgggcgaa ccgaaaaccc aataccgcct
     4921 gcgcgactgg ggcatttcgc gccaacgcta ctggggctgc ccgattccca tcgtccattg
     4981 cgaacaatgc ggcgacgtac ccgtccctgc cgaccaattg ccggtcgttt tgcctgaaaa
     5041 cgtcgtaccc gacggcatgg gttcgccgct ggcaaaaatg cccgagtttt acgaaactgc
     5101 ctgcccatgc tgcggcggcg cggcaaaacg cgaaaccgac accatggaca ccttcatgga
     5161 atcgagctgg tatttcttcc gctatatgtc gccaaaattt tcagacggca tggtcgatcc
     5221 tgccgccgcg aaatactggg gcgcggtcga ccaatacatc ggcggcatcg aacacgcgat
     5281 tttgcacctc ttgtacgcgc gtttcttcac caaactgatg cgcgacgaag gtctagtcaa
     5341 tgttgacgaa ccgtttgaac gcctgctgac gcaaggcatg gtcgtttgcg aaacctacta
     5401 ccgcgaaaac gacaaaggcg gcaaagattg gatcaacccc gccgatgtcg agctgacgtt
     5461 cgacgacaaa ggccgcccca tttccgccgt cctcaaagcc gacgggctgc ccgtcgtcat
     5521 cagcggcacg gaaaaaatgt ccaagtccaa aaacaacggc gtcgatccgc aagaactgat
     5581 taacgcctac ggcgcggaca ccgcccgcct gtttatgatg ttcgccgcac cgcccgaaca
     5641 gtccctcgaa tggagcgaca gcggcgtcga aggcgcacac cgcttcctgc gccgcctgtg
     5701 gcgtaccgtt tacgaatact tgaagcaagg cgaagcggtc aaagcgttcg caggcagcca
     5761 agacggtttg tctaaagaac tcaaagacct gcgccacaaa ctgcacgcga ccactgccaa
     5821 agtcagcgac gactacggcc gccgccagca gttcaacacc gccatcgccg ccgtgatgga
     5881 attgctcaac caatacgaca aaaccgacac cggcggcgaa caaggacgcg ccgtcgccca
     5941 agaagtattg gaaaccgccg tacgcctgtt gtggcccatc gtgccgcaca tctgcgaaac
     6001 cctgtggagc gaattgaacg gcgcgaaact gtgggaagca ggctggccga cagtcgacga
     6061 agccgccttg gtcaaatccg aaatcgaagt gatggttcaa gtcaacggca aactgcgcgg
     6121 caaaatcact gttgccgccg acgcatccaa agccgacctc gaagccgccg cactcgccac
     6181 cgaaggcgcg gtgaaattca tggaaggcaa accggcgaag aaaatcatcg tcgtaccggg
     6241 cagactggtg aacatcgtcg tctaaccgct tttaaggttt agccacacgg ataaaggccg
     6301 tctgaaactt ggaaacaggg tttcagacgg cctttttaag gcagattggg ttgtctgcat
     6361 tacagacctc aaatatagtg gattaacttt aaaccggtac ggcgttgccc cgccttgccc
     6421 tgctgtctgc ggcttcgtct ccttgtcctg atttttgtta atccactaaa acttaatctc
     6481 aaaatccaaa acatgaattt atttttcgat accgaattgg gaaagcaaca aaataaagca
     6541 acccataaaa tccgtgtaat gagcgaggct tggctggaaa aaaacggcta ctgtccctgt
     6601 tgcggaagca agccgatgca gagatttgcc aataacaaac ctgttgcaga cctcttttgc
     6661 ccaaattgcc acgagcaata tgaattaaag agtaaaaatc aaaaaaccat aggcaacagt
     6721 gtgcctgacg gtgcatatcg caccatgttg gagcgcatcc ggtcagatac caacccaaac
     6781 tttttctttc ttgcatataa aaaagcggat tactccatac ggcaattggt gcttgtaccc
     6841 aaacatttca tcacgccgga tatgattatt cccagaaata aaggcattaa aaaccggccg
     6901 aaccacatta tgtgttccat caatctcgcc cctttgcctg aaagcggaaa aatattctta
     6961 atagacgatt cccgcattat cgaacccgaa accgttctga aaaaatggca atccaatctg
     7021 tttttacgca accaaaatgc ggagcgcaaa ggttggcttt tggctgttat gaaatgtatc
     7081 gaccaacttc ccgaagaatt cacattgtcg caaatgtatg aatttgaaga aaaactatcc
     7141 atccaatttc cccaaaacaa ccatatcaga gacaaaatcc gccaacagtt gcaaattttg
     7201 cgcgaccaaa atacgatcga attcattggt cgcggacttt acaaaaaaat cgacaaattg
     7261 cgtccgactc ccaaggcgtt ttaatttcaa atcatgatac tgaattcaca agggatattt
     7321 attcttctgc tattctgaaa acagttgaag tacagcgcaa tattgccgca aaaggcagca
     7381 gccgtaagaa ggtcggggaa tgattggcaa tttatgcgtg attccgacaa accggccgcc
     7441 tccgaaggcg cagtgaaatt catagaaggt aatcctgcca agaaaatcat cgccgtaccg
     7501 ggcagactgg tgaacatcgt ggtgtaaatc tttttaaaaa cattaaacaa tgttaagatg
     7561 ccgtccgaaa tccgttttgg attcagacgg cattttttga atttaatttt ttaagggata
     7621 agcctaatat gaaatttcct tccttccttt aaacggatag tctgtctgtc ggcagcaatc
     7681 tcgatattgg gaggttgtgc ggtcgttgct gatgtttacg gtcatgattc cgccacaatg
     7741 aacgctgcgg ctgccaaaga ttatatgaaa acggttgagt tgaacaagtc tgccggcaat
     7801 gtcgatacca catccagaac agcccgcagg gtgcaggcag tatttcgacg tatgctgcct
     7861 tatgccgatg cggcaaataa taccggccat aagtttgact ggaaaatgac ggttttcaaa
     7921 aacgatgagc tgaacgcgtg ggcaatgccc ggcgggaaaa tggcgtttta tacggggata
     7981 gtcgacaaac tcaagctgac cgatgacgaa attgccgcca ttatggggca tgaaatgacg
     8041 cacgccctgc atgaacacgg taaaaataag gtcgggcaga aaatcttgac taatatggcg
     8101 gcgcagatag gcacgcagat
//
LOCUS       DAXHKX010000085         8062 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHKX010000085 DAXHKX010000000
VERSION     DAXHKX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.085"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            283..1452
                     /gene="prpF"
                     /locus_tag="ACFMWZ_001346"
     CDS             283..1452
                     /gene="prpF"
                     /locus_tag="ACFMWZ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC5935301.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDLYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1867..3066
                     /locus_tag="ACFMWZ_001347"
     CDS             1867..3066
                     /locus_tag="ACFMWZ_001347"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC5935302.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3173..3793
                     /locus_tag="ACFMWZ_001348"
     CDS             3173..3793
                     /locus_tag="ACFMWZ_001348"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC5935303.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(3920..4363)
                     /locus_tag="ACFMWZ_001349"
     CDS             complement(3920..4363)
                     /locus_tag="ACFMWZ_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC5935304.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4537..4905
                     /locus_tag="ACFMWZ_001350"
     CDS             4537..4905
                     /locus_tag="ACFMWZ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC5935305.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4950..6104
                     /locus_tag="ACFMWZ_001351"
     CDS             4950..6104
                     /locus_tag="ACFMWZ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC5935306.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKSVPFAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6161..7033)
                     /locus_tag="ACFMWZ_001352"
     CDS             complement(6161..7033)
                     /locus_tag="ACFMWZ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC5935307.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATFPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICIANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7118..7930
                     /locus_tag="ACFMWZ_001353"
     CDS             7118..7930
                     /locus_tag="ACFMWZ_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC5935308.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1736 a         2224 c         2338 g         1764 t
ORIGIN      
        1 tttaatccac tatactgtaa atcgtcattc ccgcgcaggc gggaatccag aaagtggaat
       61 tgaggaaacc tttttatccg atgagtttct gtgcggataa atctggattc ccgcctgcgc
      121 gggaatgacg gggtttaata atctgccgta tcacaacaca gtagccgtag attggggcga
      181 accccgacag tttgcggaat caaacggctt tggtcggagt ggcagcctaa tccactataa
      241 aaatcgtggg cagagcccac gctacataag gagaatctag aaatgccgca aattaaaatt
      301 cccgccgttt actaccgtgg cggtacatca aaaggcgtgt ttttcaaacg ttccgacctg
      361 cccgaggcgg cgcgggaagc gggcagcgca cgcgacaaaa tcctcttgcg cgtactcggc
      421 agcccggatc tctacggcaa gcagatagac ggtttgggca acgccagctc gtccaccagc
      481 aaggcggtga ttttggacaa gtccgaacgc gccgatcacg atgtcgatta ccttttcggg
      541 caagtttcca tcgacaaacc ttttgtcgat tggagtggca actgcggcaa cctcaccgcc
      601 gccgtgggcg catttgccat cgagcaaggc ttggtcgata aaggcaagat tccttcagac
      661 ggcatctgca cggtcaaaat ctggcagaaa aacatcggca aaaccattat tgcccatgta
      721 ccgatgcaaa acggcgcagt tttggaaaca ggcgattttg agctcgacgg cgtaacgttc
      781 ccggcagccg aagtacaaat cgaatttctt gatccagccg acggcgaagg cagtatgttc
      841 ccaaccggca atttggtcga tgaaattgat gtgccgaata taggccgttt gaaagccacg
      901 ctcatcaacg cgggcattcc gaccgttttc ctgaatgccg ccgacttggg ctacacgggc
      961 aaagagttgc aagacgacat caacaacgat gccgcagctt tggaaaaatt cgagaaaatc
     1021 cgcgcttacg gtgcgctgaa aatgggtctg atcagcgacg tatccgaagc tgccgcccgc
     1081 gcgcacacgc cgaaagtcgc cttcgtcgcg cccgccgccg attacaccgc ctccagtggc
     1141 aaaaccgtga atgccgccga catcgatttg ctggtacgcg ccctgagcat gggcaaattg
     1201 caccacgcga tgatgggtac cgcctctgtt gccattgcga ccgccgccgc cgtgcccggt
     1261 acgctggtca accttgccgc aggcggcgga acgcgtaaag aagtgcgctt cgggcatcct
     1321 tccggcacat tgcgcgtcgg tgcagccgcc gaatgtcagg acggacaatg gacggccacc
     1381 aaagcggtta tgagccgcag cgcacgcgtg atgatggaag gttgggtcag ggtgccggaa
     1441 gattgttttt aaattgacgt agcatgggtt tgcccgcgag ccataaacag gtcgtctgaa
     1501 aaacaagtaa acatcaaatc actgaccatt cctttccctt gccctgtggc ggaaggcggc
     1561 aaatcacaag gaagaacacg gaaaccccga taaaagacag cttcccgtat taccgtcatt
     1621 cccgcgcagg cgggaatcca gacctgtcaa tatggaggat tggcagggga aaacaggttt
     1681 cgtgagttct acattctgga ttcccgccac agcctgtcct cgcgtaggcg gggacggaat
     1741 aacgatagaa aatgcggcat atgctttgcc caaagaggcc gtctgaaaca cactccgccc
     1801 aacgcccatc ctttttcaga cgaccccaca ccaaaaaaac aaccacaaac tacaaggaga
     1861 aacatcatgt ccgaccaact cattcttgtt ctgaactgcg gcagttcatc gctcaaaggc
     1921 gccgttatcg accgcaaaag cggcagcatc gtcctaagct gcctcggcga acgcctgacc
     1981 acgcccgaag ccgtcattac gttcagcaaa gacggcaaca aacgccaagt tcccctgagc
     2041 ggccgaaatt gccacgccgg cgcggtgggt atgcttttga acgaactgga aaaacacggt
     2101 ctgcacgacc gcatcaaagc catcggccac cgcatcgccc acggcggcga aaaatacagc
     2161 gagtctgttt tgatcgacca ggccgtaatg gacgaactca atgcctgcat tccgcttgcg
     2221 ccgctgcaca accccgccaa catcagcggc atcctcgccg cgcaggaaca tttccccggt
     2281 ctgcccaatg tcggcgtgat ggatacttcg ttccaccaaa ccatgccgga gcgtgcctac
     2341 acttatgccg tgccgcgcga gttgcgtaaa aaatacgctt tccgccgcta cggtttccac
     2401 ggcaccagta tgcgttacgt tgcccctgaa gccgcacgca tcttgggcaa acctctggaa
     2461 gacatccgca tgattattgc ccacttaggc aacggcgcat ccattaccgc catcaaaaac
     2521 ggcaaatccg tcgataccag tatgggtttc acgccgatcg aaggtttggt aatgggtaca
     2581 cgttgcggcg acatcgatcc gggcgtatac agctatctga cttcccacgc cgggatggat
     2641 gttgcccaag tggatgaaat gctgaacaaa aaatcaggtt tgctcggtat ttccgaactt
     2701 tccaacgact gccgcaccct cgaaatcgcc gccgacgaag gccacgaagg cgcgcgcctc
     2761 gccctcgaag tcatgaccta ccgcctcgcc aaatacatcg cttcgatggc tgtggcctgc
     2821 ggcggcgttg acgcactcgt gttcaccggc ggtattggcg aaaactcacg taatatccga
     2881 gccaaaaccg tttcctatct tgatttcttg ggtctgcaca tcgacactaa agccaatatg
     2941 gacaaacgct acggcaattc gggtattatc agcccgaccg attcttctcc ggctgttttg
     3001 gttgtcccga ccaatgaaga actgatgatt gcctgcgaca ctgccgaact tgccggcatc
     3061 ttgtagccaa aaaagggacg agtccgcaca atgccgtctg aaattttcag acggcatttt
     3121 gtttggattt tgggcgtaca atacggaaaa catgacgata aggacacaaa ccatggcaca
     3181 gtttttcgct attcatcccg acaatcccca agaacgcctc atcaagcagg cggttgaaat
     3241 cgtcaatcaa ggcggcgtgg tggtttatcc gaccgattcg tgttacgcct tgggttgcaa
     3301 actcggcgat aaggcggcga tggaacgcat actctccatc cgcaaaatcg atttgaaaca
     3361 ccacctgacc ctgatgtgcg ccgatttgag cgagttgggc acgtacgcca aaattgacaa
     3421 cgtacagttt cgtcagctta aagccgccac gcccgggcct tataccttta tcttgcaggc
     3481 gacgaaggaa gtgccggcgc gcacgctgca cccgaaacgc aaaaccatcg ggctgcgtat
     3541 tcccgataat gccattgcac aagccctgct gggggaattg ggcgagccgc ttttaagctg
     3601 caccctgatg ctgcccgaag acggcgaacc attgaccgat ccttatgaaa tccgcgagcg
     3661 tttggaacac gccgtcgatt tggtgattga cggcggctgg tgcggaaccg agccgaccac
     3721 cgtcgtcgat atgaccgacg gcacggaact ggtgcgccgg ggttgtggcg atacggcggt
     3781 gttcggtttg tagggaaacc gatgccgtct gaagcatcgg ctgttcagac ggcattgcgc
     3841 gccttgccgg cggcagtccg aaatgccggc gcgtatcgcg ctcggtcgga atatccgttt
     3901 gaaacggcat tttgatgcat tactgcaccg caatcggaat tctcggttcg tagagcaggt
     3961 cgtaggtcgg cttgttgagc aggtcttgga gcgtgaaacc gtccagatac gtgaaaaacg
     4021 acttcattgc accgccgagt atgcctgtca gacggcagga cggtgtaatc aggcattcgt
     4081 tgttcgaacc catacattcg accagctgca tcggttcgag gtggcggacg accgagccga
     4141 tgttgatgcg gtcgggcggt gtggcaagcc gcagaccgcc gccttttccg cgcacgctgt
     4201 ggaggaagcc gcctttgacc agcgcggtaa cgaccttcat cagatggctt ttggaaatgc
     4261 cgtaggttac ggcgatggta ctgatgttga ccagcgcatc gtcgttgatg gcagtgtaga
     4321 taaggacgcg cagcccgtag tccgtatgtt gtgtcaaata catgattttc tcggtatgga
     4381 ttgttattct tatcggtacg gtttaaggtt cacggacaat accttaatgg ttgaaaccct
     4441 gtccgtcggg gcggtagaat gcagcctgtc tgcggcggta tgccgtctga aacatccgcg
     4501 ctaccgtttg ataatttgtt attgtaactc aaaatcatga aaccgttgaa acgacatccc
     4561 gcccttatcg ggctttcgcg tgaccaccac cattcgcttt ccctgtgcgt gcgtctgttg
     4621 cggacgccgg aagaaaggca tcgggacgaa ctcgaaccgc atttttccga attggaaacc
     4681 cattttcgcg aagaagaagc caagtttgcc ccaatttggc agaatgtcgc ccccgaattg
     4741 aaacaacgtt tcgagaaaga ccacgcccga ctgcggcaga tgatggcaag ccccgaatac
     4801 ggtaacgcgg cgtggaatac cgcttttgcc acaaccctgc gcgaccacgc gcgctttgaa
     4861 gaacgcgagc tgtttcccgc cgccgaaccg tttttgccgg catgattccg ttttgcggca
     4921 aatatattaa tgataaacaa ggaacacaca tgaaatttac caagcacccc gtctgggcaa
     4981 tggcgttccg cccattttat tcgctggcgg ctctgtacgg cgcattgtcc gtattgctgt
     5041 ggggtttcgg ctacacggga acgcacgagc tgtccggttt ctattggcac gcgcatgaga
     5101 tgatttgggg ttatgccgga ctggtcgtca tcgccttcct gctgaccgcc gtcgccactt
     5161 ggacggggca gccgcccacg cggggcggcg ttctggtcgg cttgactatc ttttggctgg
     5221 ctgcgcggat tgccgccttt atcccgggtt ggggtgcgtc ggcaagcggc atactcggta
     5281 cgctgttttt ctggtacggc gcggtgtgca tggctttgcc cgttatccgt tcgcagaatc
     5341 aacgcaatta tgttgccgtg ttcgcgctgt tcgtcttggg cggtacgcac gcggcgttcc
     5401 acgtccagct gcacaacggc aacctaggcg gactcttgag cggattgcag tcgggcttgg
     5461 tgatggtgtc gggttttatc ggtctgattg gtacgcggat tatttcgttt tttacgtcca
     5521 aacggttgaa tgtgccgcag attcccagtc cgaaatgggt ggcgcaggct tcgctgtggc
     5581 tgcccatgct gaccgccatg ctgatggcgc acggcgtgat gccttggctg tcggcggctt
     5641 tcgcgtttgc ggcaggtgtg atttttaccg tgcaggtgta ccgctggtgg tataagcctg
     5701 tgttgaaaga gccgatgctg tggattctgt ttgccggcta tctgtttacc ggattggggc
     5761 tgattgcggt cggcgcgtct tatttcaaac ccgctttcct caatctgggt gtgcatctga
     5821 tcggggtcgg cggtatcggc gtgctgactt tgggcatgat ggcgcgtacc gcgctcggtc
     5881 atacgggcaa tccgatttat ccgccgccca aatccgttcc gtttgcgttt tggctgatga
     5941 tggcggcaac cgccgtccgt atggttgccg tattttcttc cggcactgcc tacacgcaca
     6001 gcatacgcac ctcttcggtt ttgtttgcac tcgcgctttt ggtgtatgcg tggaagtata
     6061 ttccttggct gattcgtccg cgttcggacg gcaggcccgg ttgagacaaa ccgccgcaga
     6121 tttcgggtct gggcggtttg cttttcagac ggcagggcgg tcagttgccg tccagccagc
     6181 ggtcgcgtgt ggttttggct tcttcaaaat agcggtacag ggcttcgcgg tcgtcggcgg
     6241 tcaggatgtc tgccaaaacg tccaactgtt tgcccaagcc ttgaaccagt tgcagcaggc
     6301 tgtctttgtt ggcaatgcag atgtccgccc acacggcggg atgaccggag gcgatacggg
     6361 tgaagtcccg aaagcccgtg gcggcgaatt tcagatattc ctgtccgtcg gggtggtcga
     6421 gaatctggtg gacataggcg aaggcggtca ggtggggcat atgggagacg gcagcgaaaa
     6481 ccgcgtcgtg gcgttgcgcg tccatcgtat aaatttccgc accgaccgcg tgccacagtt
     6541 cttctaccaa ggcaatgccg tctgaatgtt cgccgccgtg tggcgtgatg atgagttttc
     6601 tgtggcggaa cagcccgaac tgcgcggctt gcgcaccgct tctgtccgaa ccggcaatcg
     6661 ggtgggcggc gatgcagcgg tgcaggcggt cgggcagaca gcggcggaag gcttcgatga
     6721 ccgaagattt ggtgctgccg acatcggaaa tccaagtgtg ttccggcaaa acggggcgca
     6781 gcgcggtcaa aatggcggga aaggtggcga cgggcgtggc aatcagtacc aagtccgcac
     6841 cgccgatgct gtccgcgtcg atggcaacgg acgcacggtc aatcacgccg cgttccaatg
     6901 cacgttcgag gttgtcgcgg tcggtgtcga taccggtaac ggtgcggacg agtccctgcc
     6961 ttttgaggtc gagaacgaac gaaccgccga tcagccctac accgatgagg gcaatatggt
     7021 tcaaaatggg catttgtgta aacggttttc gcaaagtacc gtcatggtag cctatcggcg
     7081 gaatatgccg caaggtcggc aggaaaaagg agaagaaatg gacaaaatca gagttgccgc
     7141 cgtgcagatg gtgtcgggcg tgtcgccgga aaccaacgtc gccgccatga aacgcctggt
     7201 cgcacgggcg gcggagcagg gtgtggattg ggtgctgctg cccgaatatt gggtgctgat
     7261 gggcgcaaac gataccgaca aactcgcgct tgccgagcct ttgggcggcg gacgctttca
     7321 gacggcattg agcgaaacgg cgaaagaatg cggcgtggtg ctgttcggcg gaaccgtgcc
     7381 gctgcaaagc cccgaggcgg gcaaggtcat gaatacttta ttggtgtacg ggcgcgacgg
     7441 caaacggacg gggctgtacc acaaaatgca cctcttcggc ttttccggtt tgggcgaacg
     7501 ctatgccgaa gccgatacca tccgcgcggg cggggatgtg ccgcacttgt cggcagaagg
     7561 cgtgtcggtg gcggcgggca tttgttacga tgtccgcttt cccgaatttt tccgccgcca
     7621 gctgccgttt gacgtattga tgctgcccgc tgcgtttacg cacacgacgg gcaaggcgca
     7681 ttgggagctg ctgctgcgcg cgcgtgccgt cgaaaaccaa tgttacgtcg tggcggcggc
     7741 acagggcggt ttgcacgaaa acggacggcg cacgttcgga cacagcatga ttgtcgatcc
     7801 gtggggcgac gtgttggacg tattgcccga aggcgaaggc gttgttacgg cagacatcga
     7861 tgccaaccgc ctgaacagcg tccgcaaccg cctgcccgcc ttgaaatacc gggttttgga
     7921 tgccgtctga aggttcagac ggcatcggtg ccggggaatc agcgggctgt aggatgggct
     7981 tcagcctgcc atttccatca atcccacatt tctaccgttt tcatcgaatc catcgaatcc
     8041 gcccctttcg acgacccagt cc
//
LOCUS       DAXHKX010000086         8042 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHKX010000086 DAXHKX010000000
VERSION     DAXHKX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.086"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(68..814)
                     /locus_tag="ACFMWZ_001354"
     CDS             complement(68..814)
                     /locus_tag="ACFMWZ_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC5935309.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(798..977)
                     /locus_tag="ACFMWZ_001355"
     CDS             complement(798..977)
                     /locus_tag="ACFMWZ_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935310.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(993..1664)
                     /locus_tag="ACFMWZ_001356"
     CDS             complement(993..1664)
                     /locus_tag="ACFMWZ_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC5935311.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFMWZ_001357"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFMWZ_001357"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC5935312.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFMWZ_001358"
     CDS             complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFMWZ_001358"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC5935313.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAANVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFMWZ_001359"
     CDS             complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFMWZ_001359"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC5935314.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAEGGQISATVDRSG
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFMWZ_001360"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFMWZ_001360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC5935315.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHG
                     QYLDGLGEACIWRKEAVPSENGGADA"
     gene            complement(4571..5881)
                     /locus_tag="ACFMWZ_001361"
     CDS             complement(4571..5881)
                     /locus_tag="ACFMWZ_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC5935316.1"
                     /translation="MKVEQGVRGLCKSRRFVPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSEMVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFMWZ_001362"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFMWZ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC5935317.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLLEKKEEKDEG"
     gene            complement(6056..6355)
                     /locus_tag="ACFMWZ_001363"
     CDS             complement(6056..6355)
                     /locus_tag="ACFMWZ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935318.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLAVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="ACFMWZ_001364"
     CDS             6706..7902
                     /locus_tag="ACFMWZ_001364"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC5935319.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITSADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1896 a         2420 c         1960 g         1766 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcgctgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcac gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc gcccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggttt gtcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag atgaattttt cggacgcgct ggcgacgatt ttttcgttga
     2101 ggtgcgcgcc gccaccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 cgatatagac cgccaacccc gatacttcgt tcaaagaaac gacgggaata tcgtactggg
     2221 caagcagttc gccggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atatattcat
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatgcgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatcc gcccgagata gccgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat gtgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattgg ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcgccaag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg gtttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaagccccga acggtcgaca gttgcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaacggg aactgccaaa attccgcctt cggcagcgtt
     3301 gcccgcctgt tctatcaacc gcgtcagagc ttcagacggc aggcagcatc gggcggcatc
     3361 gtgtaccaaa atattgtcgg tttccgccgc caaaccggtt tccaacaatt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacgccctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gccccgccgt tttccgacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccagttcg
     3781 gcttcatccg ccttaatcac tttcagtttg gcaccgagtt tcatttcggc agatgcaaca
     3841 ggtttggctg ccggctcttc ctcctcggcc tcccccatca ggtcgaactg ccgccgcgtc
     3901 atcgctaaat aaacttcgcc caaaagttcg cagtcgatca gcgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac agggcatcta ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcgacagtc
     4081 ggcagcccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcta aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaagcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc atttcactca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacagacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtgg
     5821 cgaaaagcgg gacgaaacgg cggcttttgc acaatccccg gacaccctgc tcaaccttca
     5881 tctttttcct ctttcttttc aagcaatttg cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggccaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg aatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat acccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 ggcgtgatta cctccgccga cagcaaagcc gttgccgtgg tcattccgac caacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctctg gaaatggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccgc ttacctgaac cttatagtgg attaaattta
     8041 aa
//
LOCUS       DAXHKX010000087         7885 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHKX010000087 DAXHKX010000000
VERSION     DAXHKX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.087"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            59..523
                     /locus_tag="ACFMWZ_001365"
     CDS             59..523
                     /locus_tag="ACFMWZ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC5935320.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            1282..1770
                     /locus_tag="ACFMWZ_001366"
     CDS             1282..1770
                     /locus_tag="ACFMWZ_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC5935321.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(1829..2557)
                     /locus_tag="ACFMWZ_001367"
     CDS             complement(1829..2557)
                     /locus_tag="ACFMWZ_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC5935322.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            2884..4302
                     /locus_tag="ACFMWZ_001368"
     CDS             2884..4302
                     /locus_tag="ACFMWZ_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC5935323.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            complement(4366..4992)
                     /locus_tag="ACFMWZ_001369"
     CDS             complement(4366..4992)
                     /locus_tag="ACFMWZ_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC5935324.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            5135..6475
                     /locus_tag="ACFMWZ_001370"
     CDS             5135..6475
                     /locus_tag="ACFMWZ_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC5935325.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            6549..>7885
                     /locus_tag="ACFMWZ_001371"
     CDS             6549..>7885
                     /locus_tag="ACFMWZ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC5935326.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILSNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
BASE COUNT         1747 a         1976 c         2126 g         2036 t
ORIGIN      
        1 tgttaatcca ctatatactc tatttgttaa aatcaataat aaatacaagg taatccaaat
       61 gaaagaacta gataaaatcg atttccgcat tcttaagatc ctccaacaga atgcccgcat
      121 cccgatgacg gagcttgccg agaaggtagg cttgtccacc acgcccgtta cagagagggt
      181 gcgccgtttg gagcgggaac attatatttc cggctatcac gcccatctca atccccatct
      241 gttgggcaaa ccactattgg tttttgtcga gctgaagctg caatccaaat cgggcaatat
      301 tttcgaagat ttcaaaaaag aagtgctgaa aattccgcaa attatggaat gccacttggt
      361 gtcgggcgaa tacgactatc tgattaaagt acgtttgccc gatatgtctg cctatcggga
      421 tatgctaggc aatatcctat tgcaactgcc tgccgcttcg gagagtagga gttatgttgt
      481 gatggaggag gtcaaagaaa atccggtttt ggatttggat tgaatatttc aggctggcta
      541 tgcggtttga cacctaaatg aaacataaca acatcacgca cagaggcttg ttgcttcaga
      601 taaaaaaatg cacacattca atagaatgtg tgccaagtct acaaaggaga aatagaggag
      661 aaactattaa ctgactactc gaaccagcta acggaacgaa ttatgcagct tttccggtca
      721 aaaagcaagc gtttttcttg ttaaatttct taaagaatgg aatttcaaac aataaaagta
      781 attaaatttc aaaaaaagcg gagataaaaa aatgcacaca tccggttgaa tgtgtgccaa
      841 gtctacaaag gagaaataga ggagaaaaat caagctgcat caagcaactc gacggcggga
      901 attatccgcc gtttcttatc cgctgtcaat tattttttga aaatttaccc gaccgattca
      961 aattttgcaa aaaacttgcc cgatggctat ccggtaacaa attccttcaa aaacccatca
     1021 atccgcttat tcaaaaaaat catatgattt cattgaaata aacttttgat tcgctaaatt
     1081 tgctgaaatt caaatcctcc ggatgtattt ttttgtgggc attgtgcggg caaatcgccg
     1141 ttttgtccct tgtcaggttt tgtgcatatc ggggaaaacc gaaatcgtcc gcacctgagc
     1201 cggtgtcgat tcgtcccttg ccatctcggg atatatccag caaaatcggc atatcggtat
     1261 tttgattggt gtgttgagaa aatgaccccg ttatttagaa aagccgtttg gctgctgttt
     1321 gccgtttcgg tctgtgcatt tgccggttct ttggcggcac agtatgtttt gggtatggag
     1381 ccttgcgttt tgtgtatcag tcagcggtta tgcgttttgg caaccgcatt gtgtacggca
     1441 attgtcctga tgtgcagacc aagaagaagg gcgggcggtt tgttcggtgc tgtctttatc
     1501 agcattccgg ccgttacggg tatttctgtt gcggcatatc agttgtggtt gcagtcgctg
     1561 ccgccgggta cggctccttc gtgcggtgcg ccgtggacgt tccgcttgaa gggctggtct
     1621 ttgtttgatt ggttcgagcc tgtcgtgcgc gggttcggaa attgtgccga accggattat
     1681 ctgttagggg ttgctttgcc tgtttggagc gcggcgtatt ttctggcggt tgtcctgacg
     1741 gtttggtggg cgtgggcaag ggctaaataa atcaatgcca tctgaaaggt tcagacggca
     1801 ttttattgta tgtctgctgt gctgcgtatc agtccagatt caatacggcg gaagtgtaaa
     1861 cgtcttgcac gtcgtccaag tcttccagcg cgtcaatcag tttttgcatt ttgacggcat
     1921 cgtcgccgga gagttcggtt tcgttttggg cgcgcatcgt aacgtcgccg tcaacagatt
     1981 tgtaacctgc cgcctccaaa gcggatttta cgcccgccca atcgtttggc gcggtaatga
     2041 cttcgatgga accgtcgtcg ttggtaacca cgtcttccgc accggcttcc aaagccgctt
     2101 ccatcagcgc gtcttcgtca acgccgggtt cgaataccaa atagccctga tgcacgaagt
     2161 tgaacgccac gcagccgtca gtacccaagt taccgccgtt tttggtaaac gcgtggcgca
     2221 cgtcggcaac ggtgcgggtt ttgttgtcgg tcaggcagtc caccatcaaa gccgcgccgc
     2281 cgatgccgta gccttcgtag cgcaattcga tgtattccac gccttccaag ttgcccgtac
     2341 ctttgtcgat ggcgcgttgc acattgtctt tgggcatatt gttttcggct gctttttcca
     2401 aagccaggcg caggcgcgga tttgaaccgg ggtcgccgcc gcccatacgc gccgcaacgg
     2461 tgatttcttt gattaaacgg gtgaagattt tgccgcgttt ggcatcctga cgggcttttt
     2521 tatgctggat attcgcccac ttgctatggc ctgccataaa tttcctttct tatttcaaat
     2581 aattaatctg tttttgtcag attttttacg gtcggcattt taacataagt tgcaagtacc
     2641 caaaatatcc tgttttatgc cgtgccgttg tcgatgccgt ctgaaagcga tttcctccgc
     2701 tttccgctta aaaataaaga aatgttttaa ttaacgatat tttacggtat gatggcgcag
     2761 cttctccccg attgttgaca atttaaggca tctgttgcgg caaatacggt ctgctcgccg
     2821 tgtttcctta ttggaatgtc ttttggaagg cagtcatttt aattgtaaga taaggtttat
     2881 tttatggaaa atatattgtc tgttctggtg ggtacagtca atcggttcct ttgggactac
     2941 ctgctgattt acgcgctttt gggtatcggc ctgtttttta cgctgtatct cggtgcgccg
     3001 cagattacca agctgggtgc gggattcaaa tctgtattcg gcggcttgtt tgccaaaggc
     3061 gataaagacg ataagtcttt atcgcagttt caggcgttgg cggttgccat atccgcgcaa
     3121 atcggtacgg gcaacgtcgc cggcgtggcg accgccatca ccgcaggcgg gccgggcgcg
     3181 attttttgga tgtgggtttc tgccgttttg gggatgtcca cgatttttgc ggaggcactg
     3241 ctggcgcaga aataccgcgt cgtcagccac ggcaaataca tcggtgggcc ggcgttttat
     3301 attacgcacg gtctgactcc gaaaatcggc aggggcgcgg cgcgtttcct gtcgggcttt
     3361 ttctccattg cgttgattat cgcattgggc tttatcggta atgcgacaca ggcaaactcc
     3421 attgcttctg ccgttaccat tgcatttgat gtgccttctt tggcagtcgg tattgtgctt
     3481 gccgtccttg cgggcatggt tgtgattggc ggcgtgaacc gtattgccaa tattgcccag
     3541 tttgtcgtgc cgtttatggc ggttgtttat attttgtgcg ccgtcgttat cctgtttgaa
     3601 ttttccgact atattgtgcc gatgttcaac cacatcttta ccgccgcctt caatcctgaa
     3661 gccgttttgg gcggtgcggc gggtatcggt atgcgcgaag cgatacgttt cggcgtggcg
     3721 cgcggtctgt tttccaacga agcaggtatg ggttcgactc cgcacgccca cgcgactgcg
     3781 gatgtgaagc atcctgtgca gcaaggtatg gcggcatttg tcggtgtgtt tatcgatacg
     3841 attttggtat gtacggcaac tgcgctgatt atcctgctga ccgatgccaa cctttcgggc
     3901 gaacagggtg cggcggttac tcaatttgcc tttaacaagg cattcccggg cttcggttcg
     3961 caattgcttg ccgtgtgtct gaccttcttc gcctttacga ccattatcgg ctggtattat
     4021 ttcggcgagt ccaacatccg ttttcttttc aggggaagac acttgggcat ctatcgcgca
     4081 ttggttctgc ttgccattgt tttgggtacg ctcggcaaag tcgatttggt ttggagcctg
     4141 tcggatatgt tcaacggttt tatggttatc cccaacctga ttgccctgtt cttgctgcgt
     4201 aaggaaatcc gtgccattta tgatgattat ttggcacaaa aaaaagcggg tcgggatttg
     4261 tcttatccgt atgaattcca cgagttccac gacaagggct gatgtccggc aaacagaaaa
     4321 cctcccaatc ttcaggaacg ggaggttttg cttttcagac ggcattcaga ttacgtaacc
     4381 gtacacgctg acaaattggg tgatgctgcc gcccaatacg aacagatgcc agattccgtg
     4441 cccgtgtcgg attttttcat cgtttacaaa ccagtaaatg ccgacactgt acagcatacc
     4501 gcctgccgcc agccaagcca gtcctgccga cgggagtgag gctgtcaggg atttcattac
     4561 cgccaagacc atccaaccca tgacgacata aatcacaata gacagcagac gtttttcgct
     4621 tttccgtccg atggtgagtt cttgtgcgat tcctgcagcc gccagcagcc aggacagtga
     4681 aaataccgtc cagcccggcc cgtttctcaa agaaaccagt gcaaacggtg tgtagcttcc
     4741 ggcaatcagc acataaatca tgcagtggtc ggtttttttc aaaatgcttt tcagttttcc
     4801 ggctgcaatt ccgtggtaca gcgaggaact caaatagagc agaagaaggc tgatgccgta
     4861 aaccgatacg ctgaagatac ggtagccgtc cccgtgtcct atggttttca gcagcatcag
     4921 cgccaaacct gccgccgcca gaatcaaacc gctcaaatgg ctgtaagtat tgaagcgttc
     4981 gcctgtatac atcgcacaat ctttcgggaa agtagaggca gtcagtttac gccgatactt
     5041 ttgcctctgt gtgggattta ctctttgcct acactgtgaa tattcttgaa aatgcgtgtg
     5101 ttaaaatctg ttttttattt tcagacggca ttttatgttg aatccatccc gaaaactggt
     5161 tgagctggtc cgtattttgg aagaaggcgg ctttattttc agcggcgatc ccgtgcaggc
     5221 gacggaggct ttgcgccgcg tggacggcag tacggaggaa aaaatcatcc gtcgggcgga
     5281 gatgatcgac aggaaccgta tgctgcggga cacgttggaa cgtgtgcgtg cggggtcgtt
     5341 ctggttgtgg gtggcggcgg cgacgtttgc gttttttacc ggtttttcag gtacttatct
     5401 tctaatggac aatcagggtc tgaatttctt tttggttttg gcgggcgtgt tgggcatgaa
     5461 tacgctgatg ctggcagtat ggttggcaat gttgttcctg cgcgtgaaag tggggcgttt
     5521 tttcagcagt ccggcgacgt ggtttcgggg caaagaccct gtaaatcagg cggtgttgcg
     5581 gctgtatgcg gacgagtggc ggcaaccttc ggtacgttgg aaaataggcg caacgtcgca
     5641 cagcctgtgg ctctgcacgc tgctcggaat gctggtgtcg gtattgttgc tgcttttggt
     5701 gcggcaatat acgttcaact gggaaagcac gctgttgggc gattcgtctt cggtacggct
     5761 ggtggaaatg ttggcatggc tgcctgcgaa actgggtttt cccgtgcctg atgcgcgggc
     5821 ggtcatcgaa ggccgtctga acggcaacat cgccgatgcg cgggcttggt cggggctgct
     5881 ggtcggcagt atcgtctgct acggcatcct gccgcgcctc ttggcttggg tagtgtgtaa
     5941 aatccttttg aaaacaagcg aaaacggctt ggatttggaa aagccctatt atcaggcggt
     6001 catccgccgc tggcagaaca aaatcaccga tgcggatacg cgtcgggaaa ccgtgtccgc
     6061 cgtttcgccg aaaatcgtct tgaacgatgc gccgaaatgg gcggtcatgc tggagaccga
     6121 atggcaggac ggcgaatggt tcgagggcag gctggcgcag gaatggctgg ataagggcgt
     6181 tgccgccaat cgggaacagg ttgccgcgct ggagacagag ctgaagcaga aaccggcgca
     6241 actgcttatc ggcgtgcgcg cccaaactgt gcccgaccgc ggcgtgttgc ggcagatcgt
     6301 ccgactttcg gaagcggcgc agggcggcgc ggtggtgcag cttttggcgg aacaggggct
     6361 ttcagacgac ctttcggaaa agctggaaca ttggcgtaac gcgctggccg aatgcggcgc
     6421 ggcgtggctt gagcctgaca gggcggcgca ggaagggcgt ttgaaagacc aataaactca
     6481 cttgaaatgt tctgttaagc cgccattctg aaaaaagacc gtctgaaaat ccattcttca
     6541 aaaacatcat gaacaaacaa cccctttccc tcgccgtcgt cgggcatacc aacaccggca
     6601 aaacctcgct ccttcgtacc ctgttgcgcg acagcggttt cggcgaagtc agaaacgccc
     6661 catcgaccac gcgtcatgtc gaagaagccg ccatcagcga cggcgcagac acgctggttt
     6721 tcctgtacga cacgcccgga ctcgaagacg caggcggcgt gctggaatgg ctggaaaacc
     6781 atacggacaa tcgttcagac ggcatcgaac ggctgcaaca gtttctcggc agccacggcg
     6841 cgcaccatga tttcaatcag gaagccaaag tcttacggca agtcttgcaa agcgatatgg
     6901 caatgtacgt catcgacgcg cgcgaacccg tcctcgacaa atacagggac gagttgacca
     6961 tcctttcatg gtgtgccaaa ccggttatgc ccgtgttcaa ctttaccggc ggacagcctc
     7021 ccgaatcgtg gacaaccatg ctggcgagga gaaacctgca cgttttcgca gggttcgaca
     7081 ccgtcgcctt tgattttgaa ggcgaactac gcctgtggga aaacctcgcc accatgttgc
     7141 ccgaacgcag cacacttgac cgcctgacag ccatgcgccg gcgcgaatgg cagcggctgg
     7201 acggcgaagc gcgccgcgaa atcgccgact ttttaattga tgccgccgcc ttcaggcagg
     7261 aagtggacga aaacgaggat accgccaccg tgctacaaac catgcaggcg gaaatacgcc
     7321 aactcgaacg gcagatgcag cagcggctgt ttgccctcta ccgtttctac cacagcgaaa
     7381 tcgacggcgg cgactggatg ccgcaagcct tccgccaaga cccgttcgac agcgaattgc
     7441 tcaaacaata cggcatccgc accggcacgg gcgcggcaac cggcgcactc atcggcttgg
     7501 ggctggacat cgccacactc ggcggctcgc tcggattggg tacggcaatc ggcggctttt
     7561 tgggcggcat cctgtccaat acccgcacca tttccgacaa actcgccggc cgccaaacct
     7621 tgcacaccga ccccgaaacc ctgaccctgc tcgccgcccg cgccctcgat ctgctccacg
     7681 tcctgcaaac gcgcggacac gcggcacagt cggatattga gctgcacagc cgcaaagccc
     7741 cgtgggacgc cgccagactc ccgcccgaac tcaacaaagc ccgcagtcat tggaaatggt
     7801 cgtcgctcaa tacgcaccgc cccgaaacca gccgcgccga acgcgccgga tatgtggaga
     7861 aacttcaaat ccgcctgtcg ggaaa
//
LOCUS       DAXHKX010000088         7840 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHKX010000088 DAXHKX010000000
VERSION     DAXHKX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.088"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(173..589)
                     /gene="dksA"
                     /locus_tag="ACFMWZ_001372"
     CDS             complement(173..589)
                     /gene="dksA"
                     /locus_tag="ACFMWZ_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC5935327.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(736..1545)
                     /gene="proC"
                     /locus_tag="ACFMWZ_001373"
     CDS             complement(736..1545)
                     /gene="proC"
                     /locus_tag="ACFMWZ_001373"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC5935328.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLQKELGVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTL
                     SRYLGGTRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1576..1983)
                     /locus_tag="ACFMWZ_001374"
     CDS             complement(1576..1983)
                     /locus_tag="ACFMWZ_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC5935329.1"
                     /translation="MEIRAIKYTATAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRAKEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1983..2669)
                     /locus_tag="ACFMWZ_001375"
     CDS             complement(1983..2669)
                     /locus_tag="ACFMWZ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC5935330.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2789..3832
                     /gene="pilT"
                     /locus_tag="ACFMWZ_001376"
     CDS             2789..3832
                     /gene="pilT"
                     /locus_tag="ACFMWZ_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC5935331.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3995..5221
                     /locus_tag="ACFMWZ_001377"
     CDS             3995..5221
                     /locus_tag="ACFMWZ_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC5935332.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5507..7657
                     /gene="yccS"
                     /locus_tag="ACFMWZ_001378"
     CDS             5507..7657
                     /gene="yccS"
                     /locus_tag="ACFMWZ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC5935333.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1930 a         2596 c         1693 g         1621 t
ORIGIN      
        1 gtatacggtc aatacttcat cttgcttttt ttaattattt tctccatctc gttgtctgat
       61 aaggtttttt atttcgccac aaaccacatc cgactcgaaa tttacctgcc gccatcttaa
      121 tctgcttccc tttttttctg tcatatggtt tgaaaataac caaaaggcaa atttagtcgg
      181 caaactgttt tttcatcctc tctcggcgtt cttgggactc aacagataaa gtggctgtcg
      241 ggcgtgccag cagccgcttc agaccgatag gctctcccgt atcggcacag aatccataat
      301 ccccttcatc aatattgcgg atggtcgcct gtattttact gagaagtttt cgttcccgat
      361 cgcgggtacg gagttccaat gcgtactctt cttcctgtgt ggcacggtcg gcaggatcgg
      421 gggctgattc gtgttcttgg agatgccctg tcgtagcgga agcattttcg atgagttcgt
      481 cttgcatttt taccagcaat tcgcggaaaa aagccaaatg gtcgtcattc atataatcgt
      541 cttccggccc gctccaattc aaaatatctt gttctgtcag ctttgccata atgtatatcc
      601 tctcggttca ccgaaaaaaa caaagcacca caaagtggct ttattccttt gatttttata
      661 aggcttattg cctaaggcgt ttttgaaaac gcgcatcata acacgttttg gattggtttt
      721 tttattttct ttacattatt gatattgccg ttccatttcc tgcgaacggc gcacacaggc
      781 acaaacgccc tcacttatgg cttcggcgac acgatgccgc ctgaaggctt ccacggcttc
      841 gtgggttgtc ccgccttttg acgttacatt tttttgaagc ttctcgaaat cttcacccgt
      901 ctgctcggca agggcaaccg ctcctttaaa cgttgccaga ctgagcgcgc gtgcttctgc
      961 catatcaaac ccttgtcgga tggcggcatt ttgcaatgcg tccagcagat aaaacacata
     1021 tgccggcccg ctgccgctga taccggtaat gctgtgcatt tgcgcttcat cttccaacca
     1081 aacagtcaaa ccgactgatt tcatgattcg atcggcaatc ctgcggtctg tttccgatac
     1141 ttccgcttcg gcatacatac cggatacgcc cagcccgatt tttccgggtg tattcggcat
     1201 aacccggaca atgcggcgtg ttcccccgag gtaacggctg agcgtatcga ccggcaatcc
     1261 ggctgcgaca gaaagcacca atgcgccgtt ggtgcggata tttttgcacg cggcttccat
     1321 atcctgcggc ttgacggcaa ggattaaaac atcgtcggaa tgaagctccg gcaaggtttc
     1381 agaagtttcg acccccaact ctttttgcaa acgttcgcgc ttttccgcac cccggttggc
     1441 aatatggatg cggtaaccgc cttgtttgac caatccgccc gcaacggcag ccgccatatt
     1501 gccgccgccg agaaaataaa cattcattat ttctacccta ttcaaaatta cctgactaaa
     1561 ttcattccca attttttacc atcggacgcg ctcggacaga ccgttgcgcc gcaacccctg
     1621 ctttttcaaa caatcaaaac cttcctgttt gaacgtctca atcaaaggtt tgccgtcacg
     1681 cgtacgccaa tccaacccgc aggcttcaaa cttccctatt ttcctgtaaa agtaagattg
     1741 ttgtgcggat tcatattcct ttgccctgac ggaacgattg ccgtctgaaa gcggtatctc
     1801 gtagtcctct aaagacggcg gactctcgcc gccaatatcc caaaaatcga tggcggccgg
     1861 ttttctcggc ttacaccagc cggacaggga cgaacactca taccaacccg ccaaccggca
     1921 gcctgcaacc gtaaaggcaa gcaacgcagc cgttgccgta tattttattg cccgaatttc
     1981 catcatcccc ttttcccgaa aatcgcgctg ccgatacgga cgtgtgtcgc accgcactca
     2041 atggcggcag gcatatcgtc cgacatcccc atagacagca cgtccgcctt aacgccagcc
     2101 gtattgaggt cggcaagcaa cttttgcatg gtctgaaact gggttttcaa ctccgtttca
     2161 ctgctgttgg ctttggcaac gcacatcagt ccgcgcacga caaggttggg caacttcgcc
     2221 acttcgaccg caagcgcgac cgcttcttcg ggagcaacac cgtgctttgc cgcctcgcct
     2281 gcaatattca cctcgataca cacctgcaaa ggcggcattg aagaaggacg ctgcccactc
     2341 agccggacgg cggtctttag acggcatacg gtatgcaccc aatgcgcgcg ttcggcgaca
     2401 aacttggttt tgttggactg cacatcgccg atgacgtgcc acacgatgtc ggtcaaatcc
     2461 gccaactctt ccgttttgcc gtaccactcc tgaatatagt tctcgccgaa atcacgctgt
     2521 ccggcggcgt aaacttcgcg gatgccgtct gaagggaaag tcttaccgac ggcaatcagg
     2581 ctgacggaat gcggcttcct tcccgcctgc agaaccaatt ttccaatacg gtcggacacc
     2641 tcccgataac gttcttgcaa caccgtcata gattatcccc taattaaaaa tgattaaaca
     2701 gttgaaaccc ctccagtcag ggcggtacaa tcaaggttgt tagaaccatt ccaaccaatc
     2761 gaaacattat actaaacaga gccgcattat gcagattacc gacttactcg ccttcggcgc
     2821 taaaaacaaa gcatccgacc ttcacctgag ttcgggcata tcccctatga ttcgggttca
     2881 cggcgatatg cggcgcatca accttcccga aatgagcgcg gaagaggtcg gtaatatggt
     2941 aacttcggtg atgaacgacc accagcggaa aatctaccag caaaacttgg aagtcgactt
     3001 ctcgttcgaa ctgcccaacg tcgcccgatt ccgcgtcaac gccttcaaca ccggccgcgg
     3061 tcccgccgcc gtattccgca ccattcccag caccgtctta tcgctggaag aattgaaagc
     3121 cccgagcatt ttccaaaaaa tcgcagaatc gccgcgcggc atggtattgg ttaccggccc
     3181 taccggttcg ggcaaatcga ccacgcttgc cgcgatgatc aactacatca acgaaaccca
     3241 gccggcacac atcctgacca tcgaagaccc gatcgaattc gtccaccaaa gcaaaaaatc
     3301 cctgattaac cagcgcgagc tgcaccagca caccctcagc ttcgccaacg cgctgcgttc
     3361 cgcattgcgc gaagaccccg acgttatcct tgtcggcgag atgcgcgacc ccgaaaccat
     3421 cggcttggca ctgaccgccg ccgaaaccgg acacttggtt ttcggcacgc tgcacacgac
     3481 cggcgcagca aaaaccgtcg accgtatcgt ggacgtattc ccggcgggag aaaaagaaat
     3541 ggtgcgctct atgctgtccg aatcgctgac cgccgtcatc tcccaaaacc tgctgaaaac
     3601 gcacgacggc aacggccgtg tcgcctcgca cgaaatcctg attgccaacc ccgccgtccg
     3661 caacctcatc cgcgaaaaca aaatcacgca gattaactcc gtcctgcaaa ccgggcaggc
     3721 gagcggtatg cagacaatgg accaatcgct gcaatcgctg gtgcgccaag ggctgatcgc
     3781 accggaagtc gcacgcagac gcgcgcaaaa cagcgaaagt atgagtttct gacacacaac
     3841 cgccttccgg ccataccggc gggaaaacaa ggcgcaaaca cgcggggcgg gacgcagcat
     3901 cccgcccggc tacctttccg aacaagaagc gtccgccttc ctgttgaaac ctgccgccgc
     3961 aaactgcaag gcttaaaccg aaaagaagct aacgatgaat accgataacc tgcacgacat
     4021 cttggacgaa atggttcaag tgtattctca aaaaaaacaa agccgatccg aaaccccggc
     4081 cgaaatcggc gcacacttcc acccgctgct cgaccgcctg tgcgaaaccg cagaagcaca
     4141 aaacgcgtcc gacatcctta tcagcaaagg attcccgccc tcgttgaaaa tcaacagcgc
     4201 attaaccccg cagccgcaaa aggcgctgac aggcgaggaa accgccgcca tcgccgcatc
     4261 gacgatgaac gccgaacaat cggaaatatt ccggcgcgac ggcgaaatca actactccgt
     4321 ccagtcgcgc agcggcacgc gctaccgcgc caacgcctac cacagccaag gcagcgcagg
     4381 tttggttttg cggcgcatca accacgtcat cccgcaaatg caggaattgg gcctgcccga
     4441 aaaactcaaa gacctcgccg tcgcaccgcg cgggctgctg attatcgtcg ggcctaccgg
     4501 ttcgggcaaa tccaccacga tggcgactat gctcgaacac cgcaacaaaa ccctgcccag
     4561 ccatatcgtt accatcgaag acccgattga gtttatctac aaaccgcgcc gctgcatctt
     4621 tacccagcgc gaaatcggcg tcgacaccat aaactggcag acggcggtac aaaacgctat
     4681 gcgccaatcc cccgacgtgg tctgcatcgg cgaagtccgc agcagggaaa gtatggaata
     4741 cgcgatgcag ctcgcccaaa ccggccacct gtgcattttt acgctccacg ccaacaccgc
     4801 gccgcagtcg ctcgaacgca tactcaactt ctaccccaaa gaacagcaca accaaatact
     4861 gatcgacatc gccctcaacc tgaccggcat catctgccaa cgcctcgccc tcaaacaaga
     4921 caaaacgggc aggacggcgg ttgtcgactt gctcatcaac acgcccgcca tccaagactt
     4981 catcctgaag ggcgacctga tgaacatcag taaaatcatg gaaaccgcca aaaccgacgg
     5041 aatgcagacg atggatcaaa accttttcga actgtaccgt cacggcatca tcagttacga
     5101 agaagccctg cgccagtccg tttccgccaa caacctgcga ttgcacatcc aactgcacaa
     5161 agaaggcaaa acgcccgaac tcctttacga cagggtcaac ggtctcaacc tcatttcctg
     5221 atccgcaaaa cccaatgccg tctgaaaacc gcatccccgt tttcagacgg catgatttta
     5281 tccgccccat tcatgtgcta cactttatag tggattaaat ttatagtgga ttaacaaaaa
     5341 tcaggacaag acgacgaagc cgcagacagc acaaatagta cggaaccgat tcacttggtg
     5401 cttcagcacc ttagagaatc gttctctttc agctaaggcg aggcaacgcc gtactggttt
     5461 ttgttaatcc actatattca cttcataaca ataaaccggt aaaaccatga aaaccccact
     5521 cctcaagcct ctgctcatta cctcgcttcc cgttttcgcc agtgttttta ccgccgcctc
     5581 catcgtctgg cagctaggcg aacccaagct cgccatgccc ttcgtactcg gcatcatcgc
     5641 cggcggcctt gtcgatttgg acaaccgcct gaccggacgg ctgaaaaaca tcatcgccac
     5701 cgtcgccctg tttaccctct cctcgctcac ggcacaaagt accctcggca cagggctgcc
     5761 cttcatcctc gccatgaccc tgatgacctt cggctttacc attttaggcg cggtcgggct
     5821 gaaataccgc accttcgcct tcggcgcact cgccgtcgcc acctacacca cacttaccta
     5881 cacccccgaa acctactggc tgaccaaccc cttcatgatt ttatgcggca ccgtactgta
     5941 cagcaccgcc atcatcctgt tccaaatcat cctgccccac cgccccgtcc aagaaagcgt
     6001 cgccaacgcc tacgacgcac tcggtggcta cctcgaagcc aaagccgact tcttcgaccc
     6061 cgatgaggca gcctggatag gcaaccgcca catcgacctc gccatgagca acaccggcgt
     6121 catcaccgcc ttcaaccaat gccgttccgc cctgttttac cgccttcgcg gcaaacaccg
     6181 ccacccgcgc accgccaaaa tgctgcgcta ctacttcgcc gcccaagaca tccacgaacg
     6241 catcagctcc gcccacgtcg attatcagga aatgtccgaa aaattcaaaa acaccgacat
     6301 catcttccgc atccaccgcc tactcgaaat gcagggacaa gcctgccgca acaccgccca
     6361 agccctgcgc gcaagcaaag actacgttta cagcaaacgc ctcggccgcg ccatcgaagg
     6421 ctgccgccaa tcgctgcgtc tcctttccga cggcaacgac agccccgaca tccgccacct
     6481 gcgccgtctt ctcgacaacc tcggcagcgt cgaccaacaa ttccgccaac tccagcacaa
     6541 cggcctgcag gcagaaaacg accgcatggg cgacacccgc atcgccgccc tcgaaaccgg
     6601 cagcctcaaa aacacctggc aggcaatccg tccgcagcta aacctcgaat caggcgtatt
     6661 ccgccatgcc gtccgcctgt cccttgtcgt tgccgccgcc tgcaccatcg tcgaagccct
     6721 caacctcaac ctcggctact ggatactgct gaccgccctt ttcgtctgcc agcctaacta
     6781 caccgccacc aaaagccgcg tccgccagcg cattgccggt accgtactcg gcgtaatcgt
     6841 cggctcgctc gtcccctact ttaccccctc cgtcgaaacc aagctttgga tcgtcatcgc
     6901 cagtaccacc ctctttttca tgacccgcac ctacaaatac agtttctcca ccttcttcat
     6961 taccattcaa gccctgacca gcctctccct cgcaggtttg gacgtatacg ccgccatgcc
     7021 cgtgcgcatc atcgacacca ttatcggcgc atcccttgcc tgggcggcgg tcagctacct
     7081 atggcccgac tggaaatacc tcacgctcga acgcaccgcc gcccttgccg tatgcagcaa
     7141 cggcgcctat ctcgaaaaaa tcaccgaacg cctcaaaagc ggcgaaaccg gcgacgacgt
     7201 cgaataccgc gccacccgcc gccgcgccca cgaacacacc gccgccctca gcagcaccct
     7261 ttccgacatg agcagcgaac ccgcaaaatt cgccgacagc ctgcaacccg gctttaccct
     7321 gctcaaaacc ggctacgccc tgaccggcta catctccgcc ctcggcgcat accgcagcga
     7381 aatgcacgaa gaatgcagcc ccgactttac cgcacagttc cacctcgccg ccgaacacac
     7441 cgcccacatc ttccaacacc tgcccgaaac cgaacccgac gactttcaga cagcactgga
     7501 tacactgcgc ggcgaactcg acaccctccg cacccacagc agcggaacac aaagccacat
     7561 cctcctccaa cagctccaac tcatcgctcg gcagctcgaa ccctactacc gcgcctaccg
     7621 acaaattccg cacaggcagc cccaaaatgc agcctgaaaa agtttcggca ttttgtaaga
     7681 gaggacagat tgtcagacag gttacaagat agtggataaa gctttgcgca gggtaaatgc
     7741 gtagcaactg aaccgtcatt cccacgaacc tacatcccgt cattcccacg aaaacagaaa
     7801 accaaaaaca gaaacctaaa atcccgtcat ttccacgaca
//
LOCUS       DAXHKX010000089         7819 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHKX010000089 DAXHKX010000000
VERSION     DAXHKX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.089"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(1..156)
                     /locus_tag="ACFMWZ_001379"
                     /pseudo
     CDS             complement(1..156)
                     /locus_tag="ACFMWZ_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(313..1614)
                     /locus_tag="ACFMWZ_001380"
     CDS             complement(313..1614)
                     /locus_tag="ACFMWZ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC5935334.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPNLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQYADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(1604..2515)
                     /locus_tag="ACFMWZ_001381"
     CDS             complement(1604..2515)
                     /locus_tag="ACFMWZ_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC5935335.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            2623..3021
                     /locus_tag="ACFMWZ_001382"
     CDS             2623..3021
                     /locus_tag="ACFMWZ_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC5935336.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            3070..3675
                     /locus_tag="ACFMWZ_001383"
     CDS             3070..3675
                     /locus_tag="ACFMWZ_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC5935337.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            3861..5972
                     /locus_tag="ACFMWZ_001384"
     CDS             3861..5972
                     /locus_tag="ACFMWZ_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC5935338.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSINTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDAQNNPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVIQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKY
                     WRSDAMPGAPRTYTARVNYSF"
     gene            6175..6834
                     /locus_tag="ACFMWZ_001385"
     CDS             6175..6834
                     /locus_tag="ACFMWZ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC5935339.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            7005..7700
                     /locus_tag="ACFMWZ_001386"
     CDS             7005..7700
                     /locus_tag="ACFMWZ_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFC5935340.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         1916 a         1987 c         2089 g         1827 t
ORIGIN      
        1 ggtaatgtgc agtttctcga tatagccttc tgcatcggta cgggtatgtt gtttgtaacc
       61 gagtttgtag aggccgtttt tcttgatcca acgagcatcg ctgtccttac tcggtgtggt
      121 ttggccgctg atttgtcctt cttcatcgac ttctattttt gccgacgatg ggaacggtgg
      181 cggcttgggt ggcggtgatt tgggcatacc tgatgattga aagtgaaaaa aacggaagat
      241 attgagtcat tcggacgcaa tgccgtctga aacggaagtt cagacggcat ttgttttagg
      301 ttgccgtacc gcttagggaa taccggcgac aggataggcg ggatagccgt gggtatcgac
      361 cgaacaggca aaccgccaag gcgtgtggac ggtgtcggcg gacaggtggg cgagttcggg
      421 aatgtgccgt ttgataaaag taccgtccgg gtcggtacgg tatgaggcgg cggcaatgtc
      481 ggggcaggtg tgccgtgagg cggcaagccg ccagttgcct tggttgattg ccgcatcgaa
      541 atcggtcagc tgccgggcaa accatatctc gccttcgcgg cgggggaggt ttaaaacgtg
      601 gcagaaaaaa tccgcgctca agcgtctcag ggcggggtgg aggctgccgg ttttgtgcaa
      661 acagcgcatc gcggcatcga taatcggaat gccggtccgg ccctgctgcc aaagcgtcag
      721 gcgcagggtg tgttccggat tgccgtctga agggtcgtca tccgcgtact gcaaggcaag
      781 ttgaaggaaa aaatcgcggc ggatgatgtt gtccgcccac gcattcagac ggcgttcgag
      841 gctttcccgc gcgagcaggc gcggcgagat gcagccggca ctcaaatacg cgcccatcag
      901 cgaagtgtgt ttgcgcgagg ggaaatcctt taaaacggag taggaatccg cctgttcgag
      961 aaaccgccgc cactgccgcc aagccgccgt ttcgccgctg ttttgcggca ggaagatgcc
     1021 gtctgaaagg gcggcaggcg gcggggcgga aaggttttcg gggaagggtt ggcggtatgc
     1081 cgcgaatagg ttcggaccgg cggggggctg cttggaaaag cggtcgagcc atacttcgcg
     1141 gtagcggtcg aaatcggtgt gtgccgtgcc gccgtcgggt atcaggtcgg ttttgccgaa
     1201 aacggcgcgg tcattgacga aggttaacgc gatgccgtgt ttgtccaatt catgccaaag
     1261 ggcgttgtcg gcgagtttgt cggcaaaagt atgggattcg tcggcgatga cggcgcggat
     1321 attgaggcgg acggcgagcc ggacgagctc ggcaggcgat gccgccgtgt agagcgggat
     1381 gccgcgccct gcaagccctt gggcgagttc ggcggcggat tggcggtaga acgcggcgcg
     1441 gcgagggttg tctgtttcgg catcgtcaat ccaaataccg ataacgggca aaccttcggc
     1501 aacggcggtg cataaggcgg cgttgtcgcg gatgcggagg ttttggcgga accagacgag
     1561 cgtgtgtgcg gcgcacgtgt ccgcataaag ggggcgggcg gtttcagacg gcatttcggc
     1621 agcctttcct gctggcgatt ttttcgttca gaaaatcgat gaagctgcgg actttcgcgc
     1681 ttaagaatgc cctgtctgca taaacggcat tcagccggtc ggtcgggacg gcgtatccgg
     1741 gcagcagcct caccagcgtg ccgcagcgca aatcgtgttc cgccgcccaa agcggctgat
     1801 aaccgatgca cgcgcccgcc ttaatcattt cgcgcatcat cagcgtgttg tcggtacgga
     1861 tgacgggggt cagttcaagc cggtattttt tgccgtccga tttgcgggtg aggtcgagtt
     1921 tctgctggtt ggtgtaggtc ggcaggacgg cgggcagccc cgccacttct tccggcgttt
     1981 ccggcacgcc gttgcgcctc aggaagtcgg gcgaggcaag cagggcaaat tcgatttccg
     2041 ccagtgggcg cgcaatcagt gacggggaca gggtttggga aacgcgcaac gccaaatcca
     2101 cgccttcggc aatcaaatcg acgtggcggt tgtccaaaat cagttctaat gccacttcgg
     2161 gataacgttc gcggtattcc gccagccagt tgcatatctg gctgccggca aaccacagcg
     2221 gcatcgttac gcgcagcagc ccctgcggtt tttccgtccc cccggcggct ttttgcgcgg
     2281 catcgtcgag cgtgtcgagc gcgtaactgc attgccggta gtattcttcc ccggctccgg
     2341 tcaggctgag gttgcggctg ttgcggtgca ggagtttggc ttggacggtg ttttccaagt
     2401 ggctgacgtg tttgcttgcc attgcggtgg agatgccgag cgcgtcggcg gcgcgggtga
     2461 agccgccgct ttggacgact tggcggaaaa ccttgaggct gaacagggtg tccatatttt
     2521 cttgtgtgga aaagttgtat caataaaagc agtatatatt tgaaaagggg aaacatctat
     2581 actctaccgc ctgaaatgaa gacaaatatc aaaggagctt ttatgtccga ttgctgcaac
     2641 cgtatccaac cggttttgct ttctgttttg cgtatcgtaa ccgcctacct gtttttgttg
     2701 cacggtacgt cgaaaatctt cgccttcccc attgaaatgg gcagcggttc gcccggcggg
     2761 ctgttgctgc ttgccggtat tttagaaatt gtcggcggca ttttgctggt gttgggcctg
     2821 tttgcgcgcc ctgccgcgtt tgttttgtcc ggccagatgg cggttgccta ttttatggcg
     2881 cacgcttccg gaaatgcttt gttcccgatt gccaacggcg gcgagtccgc agtgctgttc
     2941 tgcttcgtat tcctctatat cgcggcggcg ggcggcggag catggtcgct ggacaggctg
     3001 tttttcaagc gtaaagcctg aatcggactg cctaaagtgt attttgttga atgtttttga
     3061 ggaaaagaaa tgacccgtca atctctgcaa caggctgccg aaagccgccg ttccatttat
     3121 tcgttaaata aaaatctgcc cgtcggcaaa gatgaagttg tccaaatcgt cgaacacgcc
     3181 gttttgcaca caccttcttc gttcaattcc caatctgccc gcgtggtcgt gctgtttggc
     3241 gaagagcatg ataaggtgtg gcaatttgtc gaagacgcgc tgcgtgccgc cgtgcctgcc
     3301 gacagttttg aaccgaccgc gcaaaaattg aacctgttta aggcgggtgc ggcaaccatt
     3361 ttgttttatg aagatcaaaa tgtcgtcaaa ggtttgcagg agcagttccc tgcttatgcc
     3421 gccaactttc ccgtttgggc ggaccaggcg aacgcgatgg tgcagtatgc cgtttggacg
     3481 acacttgccg cggtcggcgt aggtgcaaac ctgcaacatt acaatccctt gcccgatgcg
     3541 gcgattgcca aagcgtggaa tatccccgaa aactggttgt tgcgcgcaca aatggttatc
     3601 ggcggtattg aaggggcggc aggtgaaaag acctttgaac cagttgcaga acgtttgaaa
     3661 gtgttcggcg cataatttcg cggtcaaaaa aatgccgtct gaaccctgtt cagacggcat
     3721 ttttcagtat caggcggcga gttttccgca ttctgagacc tttgtttaca aatatcatgt
     3781 tcaatatagt taaaagaaat tattctcatt tcctccgtga ggcaatataa ttcggttgtt
     3841 ttgttaaatt gagtataaaa atgaaaatat catttcattt agctttatta cccacgctga
     3901 ttattgcttc cttccctgtt gctgccgccg atacgcagga caatggtgaa cattacaccg
     3961 ccactctgcc caccgtttcc gtggtcggac agtccgacac cagcgtactc aaaggctaca
     4021 tcaactacga cgaagccgcc gttacccgca acggacagct catcaaagaa acgccgcaaa
     4081 ccatcgatac gctcaatatc cagaaaaaca aaaattacgg tacgaacgat ttgagttcca
     4141 tcctcgaagg caatgccggc atcgacgctg cctacgatat gcgcggcgaa agcattttcc
     4201 tgcgcggttt tcaagccgac gcatccgata tttaccgcga cggcgtgcgc gaaagcggac
     4261 aagtgcgccg cagtactgcc aacatcgagc gcgtggaaat cctgaaaggc ccgtcttccg
     4321 tgctttacgg ccgcaccaac ggcggcggcg tcatcaacat ggtcagcaaa tacgccaact
     4381 tcaaacaaag ccgcaacatc ggtgcggttt acggttcgtg ggcaaaccgc agcctgaata
     4441 tggacattaa cgaagtgttg aacaaaaacg tcgccatccg tctcaccggc gaagtcgggc
     4501 gcgccaattc gttccgcagc ggcatagaca gcaaaaatgt catggtttcg cccagcatta
     4561 ccgtcaaact cgacaacggc ttgaagtgga cggggcaata cacctacgac aatgtggagc
     4621 gcacgcccga ccgcagtccg accaagtccg tgtacgaccg cttcggactg ccttaccgca
     4681 tggggttcgc ccaccggaac gattttgtca aagacaagct gcaagtttgg cgctccgacc
     4741 ttgaatacgc cttcaacgac aaatggcgtg cccaatggca gctcgcccac cgcacggcgg
     4801 cgcaggattt tgatcatttc tatgcaggca gcgaaaatgg caacttaatc aaacgtaact
     4861 acgcctggca gcagaccgac aacaaaaccc tgtcgtccaa tttcacgcta aacggcgact
     4921 acaccatcgg ccgttttgaa aaccacttga ccgtaggcat ggattacagc cgcgaacacc
     4981 gcaacccgac cttaggttac agccgcgcct ttactgcttc catcgatcca tacgaccgag
     5041 caagctggcc ggcttcgggc agattgcagc ctatcctcac ccaaaaccgc cacaaagccg
     5101 actcctacgg catctttgtg caaaacatct tctccgccac gcccgatttg aaattcgtcc
     5161 tcggcggtcg ttacgacaaa tacaccttta attccgaaaa caaactcacc ggcagcagcc
     5221 gccaatacag cggacactcg ttcagcccca acatcggcgc agtgtggaac atcaatcccg
     5281 tccacacact ttacgcctcg tataataaag gcttcgcgcc ttatggcgga cgcggcggct
     5341 atttgagcat caacacgtcg tcttccgccg tgttcaacgc cgaccccgag tacacccgcc
     5401 aatacgaaac cggtgtgaaa agcagttggc tggacgaccg cctcagcact acattgtccg
     5461 cctaccaaat cgaacgcttc aatatccgct accgccccga cgcacaaaat aatccctaca
     5521 cttgggcagt cggcggcaaa caccgttcgc gtggcgtgga attgtccgcc atcgggcaaa
     5581 tcatccccaa aaaactctat ctgcgcggtt cgttgggcgt gattcaggcg aaagtcgttg
     5641 aagacaaaga aaatcccgac cgagtgggca tccatttgaa taacaccagc aacgttaccg
     5701 gcaacctgtt tttccgttat accccgaccg aaaacctcta cggcgaaatc ggcgtaaccg
     5761 gtacaggcaa acgctacggt tacaactcaa gaaataaaga agtgactacg cttccaggct
     5821 ttgcccgagt tgatgccatg cttggctgga accataaaaa tgttaacatt acctttgccg
     5881 cagccaatct gctcaatcaa aaatattggc gttcggatgc catgcccggc gcgccgcgca
     5941 cttatacggc gcgggttaat tacagtttct aatcccgttc gtgcaacaga atgccgtctg
     6001 aaaatcctga taatccgtaa attcagcaca cgaacttttt gacaggcttt atctacatca
     6061 gtataatcca aaaagaaata gcgcccttgg cagtacgcat tacccattaa atcatagcgg
     6121 gtatgccgat cccaagggct tttttatatc tttgacaaat ataggggaag caatatgaaa
     6181 accgccattt tgattgacgg tgcatttttt attaagcggg tacggtcatt aggcctgtct
     6241 caaaatccac acgatgcaaa aagaattgct gatttggctt tccgatattc gatgctgcat
     6301 ctgaaagata aaatcaacgg cacagatcaa atggatgatt tatatagaat tttcttttac
     6361 gactgcgtgc cgtttgaaaa gaaaatgcac aatcccgtat ctcatcgtgc catagatttt
     6421 tcaaagactc cggaagccat atttcgttgc aatctgcata ccgaattgaa gaagaagcgt
     6481 aaattagcgt tacgtttagg caagctgtcg gacaatacag catggatatt aaaaccccaa
     6541 gtcatgaaaa atcttctgaa aaacccgtca actcaaatta cggaaaacga tgtcgtgctc
     6601 gatgttaaac aaaaaggtgt agatatgcgt ataggcttgg atatttcatc tattacctta
     6661 aaaaaacaag ccgataaaat catcttgttt tctggtgatt ccgattttgt cccagcagcc
     6721 aaattagcca gacgggaagg tatcgatttt attcttgatc ctatgtggaa tcatgtccca
     6781 cctgacttaa tggaacacat cgacggattg aagacaaaat gtccgaaacc gtaaattact
     6841 agaacaaagg ccgtctgaaa ataaggctaa ttatctaagc catatttcag acggccttta
     6901 taaaggctga aataaaacta aacacatttt ttcactcaaa tcgaacacgc cttcaataaa
     6961 atgccataaa atccgcacat taatctgaca cacaagagat acctatgaaa ctgaaaacct
     7021 tagctttgac ttcattgacc ctgttggcat tggccgcttg tagcaaacag gctgaaacca
     7081 gtgttccggc agacagcgcc caaagcagct catctgctcc ggcagcccct gctgagttga
     7141 acgaaggtgt gaactacact gtattgtcta cgcctattcc gcaacagcag gccggtaaaa
     7201 tcgaagtatt ggaatttttc ggctacttct gcccgcattg cgcccatctt gagccggtct
     7261 tgagcgagca catcaaaacg tttaaagacg atacctatat gcgccgggag catgtcgtgt
     7321 ggggtgatga aatgaaacct ttggcacgtt tggcggccgc agtggaaatg gccggtgaat
     7381 cagataaagc caacagccat attttcgatg cgatggttaa tcaaaaaatc aatctggccg
     7441 ataccgatac cctgaaaaaa tggctgtccg agcaaacagc gtttgacggc aaaaaagtat
     7501 tggctgcatt tgaggctcct gaaagccaag cgcgtgcggc tcaaatggaa gagttgacca
     7561 ataaattcca aatcagcggc acaccgactg tgattgtcgg cggcaaatac caagttgaat
     7621 ttaaagactg gcagtccggt atgaccacga ttgaccagtt ggtggataaa gtacgcgaag
     7681 agcagaaaaa gccgcaataa gttgaggatt gaatgagtaa aggccatctg aaaataggat
     7741 ttcagacggc cttttgtatt taggctttat agaagagatg attgcttaaa gccttatggt
     7801 tttaaatcag aatatatag
//
LOCUS       DAXHKX010000090         7736 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHKX010000090 DAXHKX010000000
VERSION     DAXHKX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.090"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            7..1885
                     /locus_tag="ACFMWZ_001387"
                     /pseudo
     CDS             7..1885
                     /locus_tag="ACFMWZ_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            1947..3242
                     /gene="tyrS"
                     /locus_tag="ACFMWZ_001388"
     CDS             1947..3242
                     /gene="tyrS"
                     /locus_tag="ACFMWZ_001388"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC5935341.1"
                     /translation="MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            3527..3694
                     /locus_tag="ACFMWZ_001389"
     CDS             3527..3694
                     /locus_tag="ACFMWZ_001389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935342.1"
                     /translation="MLVSVYLVKKERFSLMLHQVWGDVSTHSTAPAARFCAFGAFGGG
                     KFAYFSRVGRA"
     gene            3770..4690
                     /gene="ribF"
                     /locus_tag="ACFMWZ_001390"
     CDS             3770..4690
                     /gene="ribF"
                     /locus_tag="ACFMWZ_001390"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC5935343.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTLSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIAADMEAAKCW"
     gene            4831..7620
                     /gene="ileS"
                     /locus_tag="ACFMWZ_001391"
     CDS             4831..7620
                     /gene="ileS"
                     /locus_tag="ACFMWZ_001391"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC5935344.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDNPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMRVWEANSVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVLKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKEMVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFAMLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1821 a         2202 c         1996 g         1717 t
ORIGIN      
        1 aacattatga gccaagcctt accctaccgc ccggacatcg acacattgcg cgccgccgcc
       61 gttttgtccg tcatcgtgtt ccatatcgaa aaaaattggc tgccgggcgg atttctcggt
      121 gtcgatatat tctttgtgat ttcaggcttt ttgatgacgg cgatcctcct tcgcgaaatg
      181 tcgggggggg gggaggtttt ccctgaaggc attttatatc cgccgcatca aacggatttt
      241 gcccgcattt ttcgccgtat tggcggcaac gctggcaggc ggcttctttt tattcaccaa
      301 agatgatttc tttcttttgt ggaaatccgc gctgaccgcc ttgggtttcg cctccaacct
      361 gtattttgca agggggaagg attatttcga tcccgcgcag gaagaaaagc ccttgctgca
      421 catttggtct ttgtcggtcg aagaacaatt ttactttgtc tttccgatat tgctgctgct
      481 tgtcgcccgc aaaagcccgc gcgtacagtt cggcttcctc gccgcattgt gcgccttaag
      541 ccttgccgct tcctttatgc cttccgcgtt cgataaatat tacctgcccc acctgcgcgc
      601 ctgcgaaatg ctggtcggat cgctgaccgc cgtgtggatg cggtaccggc aacagcggaa
      661 tctcgccgtc ggcagatgct gtgccgccgc cggcgcattg ttttccgcgt gcatactgtc
      721 cgcctgcctg tttgcctatt cggaacaaac cgcctatttc ccagggcccg ccgctttgat
      781 tccctgtctg gctgttgccg cgctgattta tttcaatcat tacgaacacc cgcttaaaaa
      841 atttttccaa tggaaaatca ccgttgccgc cggtttgatt tcctattcgc tttatctgtg
      901 gcattggccg atattggcct ttatgcgcta tatcggcccg gacaacctgc cgccttattc
      961 gccggcggcg gcaatcgtcc tgactctggc gttttccctg atttcttatc actgcatcga
     1021 aaagccgttt aaaaaatggc aaggttcgtt cgcacaatcc gttttatgga tttatgcctt
     1081 gcctatgctc attttggggg cgggctcatt ttttgcgatg aggctgccgt ttatggcgca
     1141 atacgaccgc ttggggctga cgcgttccaa cacctcctgc cacaacaata ccggcaaaca
     1201 atgcctatgg ggggatacgg aaaaacagcc ggaactgctc attttgggcg actcccacgc
     1261 cgaccattac aaaacattct tcgatgccgt gggcaaaaaa gaaaaatggt ccgccactat
     1321 ggtttccgcc gacgcttgcg cctatgtgga aggctacgcg tcccgtgtgt tccaaaactg
     1381 ggcagcctgc cgcgccgttt atcgctatgc cgaagaacac ctgccccggt attcaaaagt
     1441 ggttttggcg atgcgctggg gcagccaaat gcccgaaaac agccgctccc ttgcctatga
     1501 tgccggtttt ttccaaaaat tcgaccgtat gctgcacaaa ctctcatccg aaaaacaagc
     1561 cgtttacctg atggcggaca accttgcctc gtcttacaac gtccagcgcg cctatatctt
     1621 gtcttcacgc ataccgggtt accgccaaac cctgcgcccg gacgacgaaa gcaccctgaa
     1681 agccaatgca cgcatcaggg aattggcagc caaatacccc aacgtctata ttattgatgc
     1741 cgccgcctat atccccgcag attttcaaat cggcggattg ccggtttact cggacacaga
     1801 ccacatcaac ccttacggcg gcacggaatt ggcaaagcgt ttttccgaaa aacaacggtt
     1861 tctcaatacg cgccataacc attgattcgc ttaaatttgt tacaatcggc ggtttgcaaa
     1921 aacgctaatt tttttgaaag agaccgatga gcgtcatcca agacctgcaa tcgcgcgacc
     1981 ttatcgcgca aacgaccgac atcgaagcct tagacgcttt gttgaacgaa caaaaaatcg
     2041 ccctttactg cggtttcgat ccgaccgccg acagcctgca catcggacac ctgctgcccg
     2101 tattggcatt gcgccgtttc caacaggcgg ggcatacgcc gattgctttg gtgggcggcg
     2161 cgaccggtat gatcggcgac cccagcttca aagccgccga acgcagcttg aattccgccg
     2221 aaaccgttgc cggctgggtg gaaagcatcc gcagccaatt aacccctttc ttgagctttg
     2281 aaggcggaaa cgccgccatt atggcgaaca acgccgactg gttcggcaaa atgaactgcc
     2341 tcgacttcct gcgcgacatc ggcaagcatt tctccgtcaa cgccatgctg aacaaagaat
     2401 ccgtcaaaca gcgcatcgac cgcgacggcg caggcatttc cttcaccgag ttcgcctatt
     2461 ccctgctgca aggttacgac ttcgccgagt tgaacaaacg ccacggcgcg gttttggaaa
     2521 tcggcggctc cgaccaatgg ggtaacatca ctgccggtat cgacctgacc cgccgcctgt
     2581 accaaaaaca agtgttcgga ctgactctgc ctttggttac caaatcagac ggcaccaaat
     2641 tcggcaaaac cgaaggcggc gcggtgtggc tgaacgcgaa aaaaacctcg ccctatcagt
     2701 tctaccaatt ctggctgaaa gtcgccgatg ccgatgtgta taaattcctg aaatacttta
     2761 ccttcctgtc catcgaagaa atcgacgcta tcgaagcgaa agataaagcc agcggcagca
     2821 agcccgaagc gcaacgcatc ctcgccgaag aaatgacccg cctgattcac ggcgaagaag
     2881 cccttgccgc cgcgcagcgc atttccgaaa gcctgtttgc cgaagaccaa agcagcctga
     2941 ccgaaagcga cttcgagcag ctcgccctcg acggcctgcc tgcatttgaa gtttcagacg
     3001 gcatcaatgt cgtcgaagcc ttggtaaaaa ccggtttggc atcctccaat aaagaagcgc
     3061 gcggctttgt gaacagcaaa gcggttttgc tcaacggcaa acctgccgaa gccaacaacc
     3121 ccaaccacgc cgccgaacgc cccgacgatg cctgcctgct gaacggcgaa cacaaacgtt
     3181 tcggcaaata cactatcctt cggcgcggca aacgcaacca cgcgcttttg gtttggaaat
     3241 aatccgattg ccgcagaaat gccgtcattc ccgcgcaggc gggaatccgg acctgtccgc
     3301 acggaaactt atcgggcaaa acggtttctt agattccacg ttctagattc ccgcctgagc
     3361 gggaatgacg agtttcaaga ttacggtgtt gtcggaacgc aactgaaccg tcattcccac
     3421 gaaagtggga atctagaatc tcggggtttg agcaactgtt tttatccgat aagtttctgt
     3481 gcggacaggt ccggattccc gcctgcgcgg gaatgacggc ggagggttgc ttgtctcggt
     3541 ttacctggtt aaaaaagaac gattttcact gatgttgcat caggtttggg gcgatgtttc
     3601 aacacatagc accgcgcctg ctgcgcgttt ttgtgcgttt ggcgcgttcg gcggcgggaa
     3661 atttgcctac ttttcccgcg tcgggcgggc gtaacgggcg gcacactgtc tataaaccgc
     3721 aataccgttt acaatgaccg cctgtttcac cacatacccg aacataacaa tgagaatcag
     3781 gctggggcgg cacaacgcgc ccgactttcc gcacggggcc gccgtaacca taggcaattt
     3841 tgacggcgta cacctcggac acaaacacat cctccaaaaa ctccgcctcg aagccgatac
     3901 gcgcggatta cccgtcgtga ccgtcgtttt cgaaccccaa cccaaagaat ttttcgcact
     3961 ccgcaccggc aggatgccac cgtgtcggat cagccccctg cgcaccaagc tcgaattatt
     4021 ggaaggcaca ggctgtgtcg atgccgtctg ggttttgcgt ttcgatcaaa atttttccga
     4081 aatatccgcg caagggttta tcgaccgcct gctgcgtcaa accttgaata cgcgttattt
     4141 gctcgtcggc gacgatttcc gtttcggtgc ggggcgggaa ggctgttttg aacttttggc
     4201 acaacagccc gatatgcaga ccgagcgtac gctttccgtc atcgtcgaag acatccgtac
     4261 cagcagcacc gccgtccgcc aagccctttc agacggcaac cttgcctatg cgaaaaaact
     4321 tttgggacac gactacgtct tgagcggcag ggtggtgcac ggcagaaaac tcggacgcac
     4381 cttaaacgcc ccgactgcca acatccgcct gcccggccac cgttatgcac tcggcggcgt
     4441 gttcgtcgtc gaagcggacg gcgcattcgg cacgcggcgc ggcgtggcga gcttcggctt
     4501 caatcccacc gttgatagcg gctgttctca aaagcttgaa gtccacctgt tcgactttca
     4561 aggcgacctg tacggacaac ggctgaacgt ccgcttcctg cacaaactgc gcgatgagga
     4621 aaagtttgac ggtatggaag aactgaaaag gcagatagca gccgatatgg aagccgcaaa
     4681 gtgttggtag aaaaacctta tacaaaccat ccgattgggc tacaatcaac cttttaactg
     4741 ttcagacggc ataggggttt cccgttgtga aatactgttt gaggcgcaat gccgtctgaa
     4801 ccgaaatatt gtaacaatag agattaaaaa atgaccgatt acagtaaaac cgtaaacctg
     4861 ctcgagagcc cgtttccgat gcgcggcaac cttgccaagc gcgagcctgc atggctgaaa
     4921 agctggtacg agcaaaaacg ctaccaaaaa ctgcgcgaaa tcgccaaagg ccgtccgaaa
     4981 ttcatcctgc acgacggccc tccgtatgcc aacggcgaca tccacatcgg tcatgccgtc
     5041 aataaaatcc tcaaagacat cattatccgc agcaaaactc aagccggttt tgacgcgcct
     5101 tatgtgccgg gttgggactg tcacggtttg cctatcgaag tgatggtgga aaaactgcac
     5161 ggcaaagaca tgcctaaagc ccgtttccgc gaattgtgcc gcgaatacgc cgccgaacag
     5221 attgcccgtc agaaaaaaga ctttatccgt ctgggtgtgt tgggcgactg ggacaatcct
     5281 tacctgacca tggatttcaa aaccgaagcc gataccgtgc gtatgctcgg cgaaatctac
     5341 aaatccggct atctctaccg cggcgcgaaa ccggttcaat tctgcttgga ctgcggttct
     5401 tcgctggcgg aagcggaagt ggaatacaaa gacaaagtat cgcctgcgat tgacgtgggc
     5461 tatccgttta aagacactgc cgcgcttgcc gccgcattcg gtttggcagg catcgagggc
     5521 aaagcgtttg ccgtcatttg gacgactacg ccttggacgc tgccggcgag ccaagccgta
     5581 tcggcgggcg cggacgtggt ttaccaactg attgatacgc ccaaaggcaa attggtgctg
     5641 gcgaaagatt tggcggaaga cgctttgaaa cgctacggct tttcagacgg catcgccatc
     5701 cttgccgaaa ccaccggcga caagctggaa aacctgcaca tgaatcatcc gttcctcgag
     5761 cgtgatattc ccatgctcaa cggcgaacac gttaccaccg acgccggtac gggtttggta
     5821 cataccgccc ctgcgcatgg tttggaagac tacgctgtct gcaataaata cggtatcgag
     5881 ctttacaacc ctgtaaatgc cgaaggcaaa tacatcagtg aaacgccacg cgttgccggt
     5941 atgcgcgttt gggaagccaa ttccgttatc ctgcaatggc tggaagaaac cggcaacctt
     6001 ttggcaagca gcaaaatcga acacagctac gcccactgct ggcgccacaa aacgccgctg
     6061 atttaccgcg cgacaggtca atggtttgtc ggtatggaca aagccggtgc cgacggtaaa
     6121 accctgcgcg acaaagccat caaagccgtg gacgacaccg aattcttccc gtcttggggg
     6181 cgcgcgcgtt tggaagcgat gatcgaaggc cgccccgact gggtggtttc gcgccaacgc
     6241 tattggggca cgccgatgac tttctttgtt cacaaagaaa cgggcgagct gcatccgaac
     6301 tctgccgaac ttttggaaaa agtcgcgcaa cgcatcgaag aaaaaggcat cgaggcttgg
     6361 ttctccctcg ataagggcga attgttgagc gcggaagatt gcgaacatta cgacaaactc
     6421 tccgacacga tggacgtatg gttcgactcc ggttcgaccc attattccgt cttgaaacag
     6481 cgcgaagaat tggactggcc ggctgatttg tatctcgaag gcagcgacca acaccgcggc
     6541 tggtttcaat cgtccatgct gaccggctgc gcctcatcaa tgggtcgcgc accgtataaa
     6601 cagctgctga cccacggttt tgttgtagac ggcgaaggca aaaaaatgtc gaaatccatc
     6661 ggcaacgtcg tcgcgccgca agaagtctat aacgaattcg gcgcggacat cctgcgtctg
     6721 tgggcggcat ctaccgatta cagcggcgaa ttggcgattt ctaaagaaat ccttaagcgc
     6781 gtaaccgaaa gctaccgccg tatccgcaat accttgagct ttttgtttgc caacttgagc
     6841 gactttaatc cgattgaaga tgccgtgcaa caggcggata tggtggaaat cgaccgctat
     6901 gccctggtgt tggcgcgtcg gctgcaagag cgtctggcag gcgattacta tccgcgttat
     6961 gccttccatt ttgccgtgaa agaaatggtt tctttctgct cggaagactt gggcgcgttc
     7021 tacctcgata tcctgaaaga ccgcctctac accaccaaag ccgacagccg cgcccgccgc
     7081 agcgcgcaaa ccgccctgta ccacatcacg cgcagcctgg ttctcttgat tgcaccgatt
     7141 ttgtgcttta ccggcgaaga agcgtgggac atcatcggcg gcggcgaaga agacagcgtc
     7201 ctcttccata cttggcacga gttcccgacc atcaacgaaa aaaccgaagc cgaactggtg
     7261 aaaaaatgga cggcaatccg cgaagcccgc gaagcggtaa ccgccgccat cgagcctttg
     7321 cgcgccgaca aaaccgtcgg ttcgtccttg caagccgaag ccgaaatcac cgcgccggaa
     7381 gaaatggccg gctacctgaa cgccttgggc gaagaattgc gctttgccat gctggtgtct
     7441 aaagcagaag tgaaagtagg cagcgaactt gccgttgccg ctaaagccag tgatggtgaa
     7501 aaatgcgaac gctgctggca ctacacccgc gatgtgggcg cggttgcagg ctatgaaacc
     7561 gtctgcaaac gctgtgcaga gaatgtcggc ggagaaggcg aaacgcgcca ttacgcctga
     7621 taaagtttga gcaaatgccg tctgaaaccg ccgaacagca tttcagacgg cattttttgt
     7681 gccgcgattt gtctttataa cggcggaggg gtttcaagat tgcggtgttg tcggaa
//
LOCUS       DAXHKX010000091         7728 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHKX010000091 DAXHKX010000000
VERSION     DAXHKX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.091"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(79..576)
                     /locus_tag="ACFMWZ_001392"
     CDS             complement(79..576)
                     /locus_tag="ACFMWZ_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC5935345.1"
                     /translation="MNNLPVADLLVSAIIAACIVLSAMRGVIAEAGSMAAWVVAFFFA
                     KLFAAPFADIAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            complement(569..1567)
                     /gene="ftsN"
                     /locus_tag="ACFMWZ_001393"
     CDS             complement(569..1567)
                     /gene="ftsN"
                     /locus_tag="ACFMWZ_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC5935346.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(1580..2854)
                     /gene="folC"
                     /locus_tag="ACFMWZ_001394"
     CDS             complement(1580..2854)
                     /gene="folC"
                     /locus_tag="ACFMWZ_001394"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC5935347.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKVGTLTSPHLLRYNERIAVNAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFHPQHSDDPTRNRNALPFPALRGAYQLSNAACTLTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHGIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(2886..3326)
                     /locus_tag="ACFMWZ_001395"
     CDS             complement(2886..3326)
                     /locus_tag="ACFMWZ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC5935348.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVSQCIIPDMTDKESYRRLKVWVLWRQPKKTAETDTPS"
     gene            complement(3331..3690)
                     /locus_tag="ACFMWZ_001396"
     CDS             complement(3331..3690)
                     /locus_tag="ACFMWZ_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935349.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDINKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            complement(3763..4491)
                     /locus_tag="ACFMWZ_001397"
     CDS             complement(3763..4491)
                     /locus_tag="ACFMWZ_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC5935350.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(4664..5443)
                     /gene="rsmA"
                     /locus_tag="ACFMWZ_001398"
     CDS             complement(4664..5443)
                     /gene="rsmA"
                     /locus_tag="ACFMWZ_001398"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC5935351.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGRADDFEYFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            5640..6329
                     /locus_tag="ACFMWZ_001399"
     CDS             5640..6329
                     /locus_tag="ACFMWZ_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC5935352.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLRERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAQMPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAVYRAF
                     FEMAGLCEKQL"
     gene            6514..7716
                     /gene="trpB"
                     /locus_tag="ACFMWZ_001400"
     CDS             6514..7716
                     /gene="trpB"
                     /locus_tag="ACFMWZ_001400"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC5935353.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFYPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTAAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
BASE COUNT         1601 a         1985 c         2196 g         1946 t
ORIGIN      
        1 ctgtgatatg gctgaggaaa ggaaaaaaca tttcagacgg cataaaagag aatgccgtct
       61 gaaatatccg tatggcaatc aatcgtcttc cggagtttcc gccgtgccgc cgctatggtt
      121 caacacggct tcggaaagcg atacgaaaaa cggcagtgtg taagattgcc gccattcttc
      181 ggtatcgggc aggtcggttt ttgaagcgag catgaccagc agggtaataa tcaaaatgcc
      241 tttcaatgca ccgaatacgc cgcccaaaat gcggttggca aagcccaaac cgaccgccga
      301 aactgccccg gtcagcagcg agcggagtat tttctggatc agacaggcaa tgacgaatag
      361 ggaaatgaac gacagagcca atgcaaacag gcggggttgg aacgatgcaa aggcgatgtc
      421 ggcgaagggt gcggcaaaga gtttggcgaa aaaaaaggca accacccatg ccgccattga
      481 gccagcctcc gcaatcacgc cgcgcatcgc ggatagcacg atgcaggcgg cgatgatggc
      541 ggagacgagg aggtcggcaa cggggaggtt attcattcgt tacctgaccg gcgataccgt
      601 gtacgcgcaa tttgttcaaa tcgcgttcgg catcccttgc gtttttatag ttgcttgatt
      661 tgacgcggta aactttgccg ttgtcggtca taatttcggt gatggtcgaa tcgatacccg
      721 ccgccttcat tttgcgctgg aggcttaagg cgcgttcttt ttcggcataa cctgcctgaa
      781 tggcggcttt tttaccggat ttttccttct cggcggtttt ggtcttgtcc gctttgtcgg
      841 ttttttgtgc cgtttcgtgt tttttgccgt ccgaacggtc tttttcggcg gtttttttgc
      901 tttcagcctt gtcggctttt ttcgcttctt ttaccgcgct gtcggatttt gccgtatccg
      961 gtttggtttt ttcggcggca gttttcggtt tgtcggcaac tttttcggcg gttttggttt
     1021 ctttggcttt gggcttggct ttggcagtgc gttccgcttt ttgcggtttt gtttcggcag
     1081 tgcgtttcgg tttttcaacc gctaccgtat ccgtactgtc ggcagttgcc ggcacttttt
     1141 cggcagcgcg ttgttttgcc tgcttcggtg cggttttggc ggtttctgcc tgttgcagtt
     1201 tctcggatgc ttccaaacct ttgatgttgc tgtcttcgag gcggtcgtta atcagcacca
     1261 gcggcgcgcc tacgttttca ggctcgctga tttcgctgtc ggcggcagaa ggcttgtctt
     1321 cgcctgccaa gtcctgcggt ttgtcggcgg cggatttcaa ggcaggggtt tgtgccgcac
     1381 ctgccgcttt gttttctacg ccgcttgttt cgccggcagt ctgttcggca gggccggaac
     1441 tgagggcggc tgccagcagg atgcaggagg cggcaaccag gcaacttgcc gttacgaggc
     1501 ggcggcggtt gcgccgtttg agttgttcgt aaccgctcag gacttcgttt tgtttgtttt
     1561 cggacataga agtttccttt taaagtaccg acatgacatc ggcaacggta tgaaatgagc
     1621 cgaaaacgac gattctgtcg ttctcgcccg cttttgaggc tgccgcccgg tatgcttcgc
     1681 ggacggcggc gaatgtctgt atgttttcga tgccgtgttc gtgcagtttg ttttgcaggc
     1741 tctcgagcgt catgccgcgc ggtacatcca acggtgcgat ataccactcg tcaaactggt
     1801 ctttaacgat ttccaacacg ccgtctatgt ctttgtcgga cagcatactg aatacggcgg
     1861 tgcgtttttg cgcaaacgcc aaattaatca ggctgcggcg cagggcgcgg gcagcgtggg
     1921 gattgtgtcc gacatccaaa acggtcagcg gacggccggg caggacttgg aagcggccgg
     1981 gattttcaac cagcagcaat ccgcgtttga tggcgccgat gtccacaggc aatttgtcgt
     2041 tcaagcattc caatacggtc aacgtgcagg cggcgttgga aagctggtat gcgccacgca
     2101 atgcggggaa gggcagggca ttgcggttgc gcgtggggtc gtctgaatgt tgcggatgga
     2161 agcggtagtt ccattggatg ttttccagcg cgtgaaactc gaaatcgcgc tgcaccatca
     2221 gcagtttcgc gcctatggct tcggcgtgtg cgaccaatga tgcgggcgcg gggttttgac
     2281 cgcagatggc gggtttgccg ctgcggaaca cgcctgcttt ttcaaagccg acctgctcga
     2341 ccgtatcgcc caaaaacgcc tgatggtcca aatccacgct ggtaaccacc gcgcaatcgc
     2401 cgtcaaatgc gttgaccgcg tccaagcgtc cgcccaagcc gacttccaat atcatcacgt
     2461 caacctgttc gcgcatgaag atgtcgacgg ccgccaaggc attgaattca aaataagtca
     2521 gcgatatttc gccgcgcgcc gcttcgatgc gctcgaaaga ggcaataatc gtatcgtccg
     2581 aaacgggttc ggcgttgacg gcgatgcgtt cgttgtaacg caataaatgc gggctggtca
     2641 gcgtaccgac tttaaagcct gcctgtttgt aaatctgtgt caggtaggcg cagaccgaac
     2701 ctttgccgtt tgttcccgcg acaacgacga cggggcattg cggcgtgagc ttcatccgtt
     2761 ttttcacttc gcctacgcgt tccaagccca tatcgatcaa gccaccgctg tgggcggttt
     2821 ccaaatggga gagccagtct tgcagtgttt tcattgagtg ttccgtgttc aaatgccgtc
     2881 tgaaatcagc ttggtgtatc ggtttcggcg gtttttttcg gctgccgcca aagcacccag
     2941 actttcagcc gccggtagga ttctttgtcc gtcatgtcgg gtatgatgca ttggctgacg
     3001 gttttgccgc cggtgtccca ttgtaagaat aaggcataag gcgtaaccat actgctgccc
     3061 gcaagtgttg ccgctgtttt gtctttgccg gatacgattt ccgcctgtcc gtcgcggtct
     3121 atggtaatgg cggttatggc atggcggtgt ttcagattcg ttatcctgag cgagtatgcg
     3181 taacttgcca ccaaagccgc caaaccgaac cacatcatcc ggccgtaaaa ccaggtcagg
     3241 cagacggcaa gggaagccga gtggagcgat acggtcagga tgttcaggat gcgggacggc
     3301 ctcaatgccg tctgaaaggc gcgcacagcc ttacatcatg ttgtcgaaca cgggggtaat
     3361 gttcaattcc gcttcttcca tgtccaacac catatcgtgg atttcgatgt cgaaaaattc
     3421 ccaaaacgcc ttcagcccca tatcttgcgg ccatttatcc ttattgatgt cccaacctgc
     3481 cagctccgcc tcgaaaatct ggcggtagcg ttcgtcgaag taggaaacga cggcttccgg
     3541 ttcgtcgaac tgcggaacga ggaagacgga acagttggca cgaagctgct ctatggtcag
     3601 gtcgggcata ttttcgtcgg tgcttttgag ccattccaaa aagcgcgcgg tcggcttgag
     3661 gacgacggcg gtgcggtcaa caaaatacat ggttttcttt ctcaatcatc ttgcggtgtc
     3721 gggatatgct gtctgaacgt tcggttttca gacggcatag catcaatggg tcatgacctg
     3781 ttgcaggaac tgcttggcgc gttcgtgttt cgggttggta aaaaacgctt ccggcgtttc
     3841 gtcttcgagg atttgccctt tatcgacaaa aagcactcgg tcggcaactt cgcgggcaaa
     3901 acccatttcg tgggttacgc acatcatcgt cataccgctt tccgccaagt ctttcatcac
     3961 tttcaacact tcgccgacca tttcggggtc gagtgcggag gtcggttcgt caaacaacat
     4021 tacgcgcggt tccatcgcca aaccgcgtgc aatcgccacg cgttgctgct ggccgccgga
     4081 aagttgggaa gggaaggcgt cttttttgtg tgccagtccg acgcgttcca aaagttccat
     4141 cgcttttttc tctgcctgtt ccgcattttg acctttaacc ttcatcggtg cgagggtaat
     4201 gttttccaac acggtcaggt gcgggtagag gttgaaactt tgaaacacaa agccgacttc
     4261 ttcgcggatt ttgttcaaat cggtttttgg gtcggcaacg ttgacaccgt ccacccaaat
     4321 ctcgccgctt tcgatgcttt caagctggtt gacggtgcgg atgagtgtgg atttgccgct
     4381 gcccgaaggt ccgcagacga cgaccacttc gccttttttg atttccaagt tcacgccgtt
     4441 gatgacgtgc aggtctttga aatgtttgtg tacgtttttg aatttgatca tgtcttttct
     4501 ttcagacggc atttgccgtt gtaggttgtc gttacgggag ctccatatga tgaagcgtgt
     4561 agcgtctgcc gtcaaaaaaa cggtcgttcg gattggtcag gcaggctgca agcggcagta
     4621 tcaggggtaa aagcaggtat ttcttcatcg gcttactccc ttttcagacg gccttgcccg
     4681 ccagataatt gctcaacgct acgtattttt ccggcgcgat atgttcggcg cggtcttgcg
     4741 gattgatgcc gactgcctgc aaatcatcgt cgcctgcaag ctctttcaga ttgttgcgta
     4801 tggttttgcg gcgttggtgg aaggcgagtt tcacgagttt ggcgaaatac tcgaaatcgt
     4861 ccgccctgcc gatgcggtgt ttcaccggaa tcatgcggac gacggcggaa tcgactttcg
     4921 gcgcggggtc gaacgattcg ggcggcacgt caatcagcat ttccatatcg aagaaatatt
     4981 gcaacatcac gcccaagcgg ccgtagtcgt tgcttttcgg cgcggcaacc atacgctcga
     5041 ccacttcttt ttgcagcata aagtgcatat cgacgacatc gtccgccacc tccgccagct
     5101 tgaacaaaag cggcgtggaa atgttgtacg gcaggttgcc gacgattttc tttttgcctg
     5161 cgatgccgtt gaaatcaaac tgcaacacgt cgccttcatg aatgacgagc ttgtccgcaa
     5221 acggcagcgt tttcagacgg catacgatgt cgcggtcgat ttcgacaacg tgcaggcggt
     5281 tcagcttttt cgccaaaggt tcggtaatcg ccgccaaacc cggaccgatt tcaatcacga
     5341 catcatccgc ctgcgggcgc acggcgttga caatatcgct gataatccgc gtgtcctgca
     5401 aaaaattctg cccgaaacgc ttgcgggctt tgtgttcttt catcgtgttt ccttttcggt
     5461 tgaaaccccg ccctttaggg cggtagaatc agactctatt tgggaggggc gtaactcctt
     5521 ccaaatcagg atggcacata gggcggtgct ttaagtgtcg tcctgtgtgt tggaacataa
     5581 atgtgtttac agtatccgtt tgatgtcggc attgtaaccg aaaacggcag ggcgtgataa
     5641 tgctgtttga aggcttgccg tgtttggcgg tttggtgcaa aaaccggctg tctgccgttt
     5701 tgcctgttgg aggattgaac gtgtctgaaa atctgcttga aatcgaaacc catcccttcg
     5761 attccgtgtt gccgccgaag gctgcggtca tgatgatggg gacgtttccg cccaaggaag
     5821 acaaacgcgc gatgcagttt cattatccga atttccaaaa cgatatgtgg cgcgtttacg
     5881 ggctggtgtt ttttaatgat gcggcgcatt tccaaaggtt gtctgaaaaa gcgtttgatg
     5941 ccgagaaaat caaggcgttt ttgcgcgaac ggggaattgc gtcctgcccg accgtgttga
     6001 aggcggcaag ggaacacggc aatgcgtccg acaagttttt aaaggtagtt gaaaccgtcg
     6061 atttggcggc ggtgttggcg cagatgcccg agtgtcgcca tatttgtacg acaggcggca
     6121 aggcgacgga aatcctgctc gatattcagg gcggcggcat taaaatgccg aaaacgggcg
     6181 aaaccgtgcc gtttccgttt gccggacggg atttgacgct gacgcgcctg ccttcgactt
     6241 cgcgcgccta tcctttgagt ttggcgaaaa aggcggcggt gtatcgggcg tttttcgaaa
     6301 tggcgggctt gtgtgaaaag cagttataat tgccgacaat ttcccgttcg gacggcatgt
     6361 ttgaaaaata tagtggatta acaaaaatca ggacaaggcg gcgaagccgc agacagtaca
     6421 aatagtacgg caaggcgggg caacgccgta ccggtttttg ttaatccact ataaattgcc
     6481 gtctgaaaat ttgaagcaca aggacgaaat ctgatgaaga actaccacgc gcccgacgag
     6541 aagggctttt tcggcgaaca cggcgggctg ttcgtgtccg agaccctgat tccggctttg
     6601 caagagctgg cggatgccta taaggcagcg aaaaacgatc ctgaattttg ggaagcgttc
     6661 cgccatgatt tgaaacatta tgtcggcagg cccagccccg tttaccacgc cgcgcggttg
     6721 tccgaacatc tgggcggcgc gcaaatctgg ttgaagcgcg aagacttgaa tcacaccggc
     6781 gcgcacaaag tcaacaacac catcggtcag gcactgttgg caaaacgcat gggtaaaaaa
     6841 cgcgtcatcg ccgaaaccgg cgcgggtcag cacggcgtgg cgagtgccac cgttgccgca
     6901 cgcttcggta tgacttgcga cgtgtatatg ggcgcggacg acatccaacg ccaaatgccc
     6961 aacgtgttcc gtatgaaatt attgggtgcg aacgtggttg gtgtagaaag cggcagccgc
     7021 acgctgaaag acgcgatgaa cgaagccatg cgcgaatggg tcgcccgcgt ggacgacaca
     7081 ttctacatca tcggtaccgc cgccggtccc gcgccgtatc ccgaaatggt gcgcgacttt
     7141 caatgcgtga tcggcaatga agccaaagaa caaatgttgg aagccatcgg cagacagccc
     7201 gacgttgccg ttgcctgcgt gggcggcggc tccaacgcca tcggtttgtt ctatccctat
     7261 atcggcgaag aaaacgtgcg cctcgtcggc gtggaggctg gcggtttggg cgtgaacacc
     7321 cccgatcacg ccgcgccgat tacttcgggc gcaccgattg gcgtattgca cggtttccgc
     7381 agctatctga tgcaggacga aaacggtcag gttttgggta cgcactctgt ttccgcaggc
     7441 ttggattacc ccggcatcgg cccggaacac agccatctgc acgacatcaa gcgcgtcgaa
     7501 tacaccgccg ccaaagacga cgaagcactc gaagcctttg acttgctctg ccgcttcgag
     7561 ggcatcatcc ccgcgctcga atccagccac gccgttgctt gggcggtgaa aaatgcgccg
     7621 aaaatgggta aagaccaagt gattttggtc aacctctcag gtcgtggcga caaagacatc
     7681 aataccgtcg cgaagctcaa agggattaaa ctgtaacctc gtccgtct
//
LOCUS       DAXHKX010000092         7533 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHKX010000092 DAXHKX010000000
VERSION     DAXHKX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.092"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(86..955)
                     /locus_tag="ACFMWZ_001401"
     CDS             complement(86..955)
                     /locus_tag="ACFMWZ_001401"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC5935354.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1050..1967)
                     /gene="hisB"
                     /locus_tag="ACFMWZ_001402"
     CDS             complement(1050..1967)
                     /gene="hisB"
                     /locus_tag="ACFMWZ_001402"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC5935355.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(1964..3061)
                     /gene="hisC"
                     /locus_tag="ACFMWZ_001403"
     CDS             complement(1964..3061)
                     /gene="hisC"
                     /locus_tag="ACFMWZ_001403"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC5935356.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3107..4396)
                     /gene="hisD"
                     /locus_tag="ACFMWZ_001404"
     CDS             complement(3107..4396)
                     /gene="hisD"
                     /locus_tag="ACFMWZ_001404"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC5935357.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4393..5292)
                     /locus_tag="ACFMWZ_001405"
     CDS             complement(4393..5292)
                     /locus_tag="ACFMWZ_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC5935358.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTKVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5396..6049)
                     /gene="hisG"
                     /locus_tag="ACFMWZ_001406"
     CDS             complement(5396..6049)
                     /gene="hisG"
                     /locus_tag="ACFMWZ_001406"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC5935359.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6128..6781)
                     /locus_tag="ACFMWZ_001407"
     CDS             complement(6128..6781)
                     /locus_tag="ACFMWZ_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC5935360.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTAMGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7058..7142
                     /locus_tag="ACFMWZ_001408"
     tRNA            7058..7142
                     /locus_tag="ACFMWZ_001408"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7092..7094,aa:Leu,seq:caa)
     gene            7397..>7533
                     /locus_tag="ACFMWZ_001409"
     CDS             7397..>7533
                     /locus_tag="ACFMWZ_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC5935361.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YL"
BASE COUNT         1579 a         1809 c         2177 g         1968 t
ORIGIN      
        1 tcataatcgg gactgatttt aagcgtgggg tgataaaaat gccgtctgaa atatgttcag
       61 acggcattgt gtttggaaaa ggcaatcagt gttctgccaa tttcaggtaa acgccggaaa
      121 cgtcctgttc gccgtagccg gcttcgactg ctttgcggta gctggcagca acggtttcga
      181 cggcgggcag ggtgttgcct gcctgttcaa gctctttgac ggcgaggttg aggtctttgg
      241 aggcgtgttt gagggcgaag gcgggtggga attcgcggtt tgcccacagg gattttttgg
      301 tttggaacat gggcgagtcc attgccgagc cgccgatggc ttcgacgatg gtgtcggtat
      361 cgatgccgaa ctgccgcgcc atcagcatgg cttcgctgta agcttcgccg aaaatgccca
      421 agagcgagtt caagacgagt ttcgcgcccg aacctttgcc gacatcgccg aaatggaagg
      481 tttttttgcc gacgagggaa aatatttttt gcagcgggtt taaaacggct tcgctgccgc
      541 cgaacagaat cagcagcgtg ccgttggtgg cgggcccgac cgatccggaa acgggtgctt
      601 cggcaaactg tccgcctgcg gcttcgacaa gtgctttgac ggcgaggttt tcggtcgggg
      661 agatggtgct catgttgacg atgattttgc cggccaatcc gtcgcggact ccgttcagga
      721 tgtcgcacac ggcggcatag tcggaaacca tcaggaaaat gacgggatag tcgcggacga
      781 gttcggcggt gttgccgtaa acttttgcgc ctttggcgga gatgggggca gttttgtcgg
      841 gcgagcggtt gtatacgccg acttcgatgc cgccgtccaa gagccgcgtt accataggca
      901 gacccatttg ccctaagcct atccagccga tttgtgtgta ttcgtttgcg gacatgatgt
      961 ttactccttt tctgtttgta gtgtagtgta atcttatctg ttccgatacc gtctgaaaag
     1021 cctgcgggtg tttcagacgg tatggttttt tatgcggtca gcgtgccttt ggtcgagggg
     1081 gtctgtcctg ccatgcgcgg gtcgtgttcg actgccatac gcagggcgcg cccgaaggct
     1141 ttgaatacgg tttccgcctg atggtgggcg tttttgccgc tgaggttgtc gatgtgcagg
     1201 gtcatcatac tgtggttgac gatgccgtgg aaaaattctt caaacaaatc gacatcgaaa
     1261 cgtccgatta gtgcgcgggt aaattcgatg ttgtacacga gtccggggcg gccggaaagg
     1321 tcgatgacga cgcggctgag ggcttcgtcg agcgggacgt aggaatgtcc gtaacggcgg
     1381 atgccttttt tgtcgccgag tgcctgccgg attgcttgtc cgagtgtgat gccgatgtct
     1441 tcggcggtgt ggtggtcgtc gatgtgcagg tcgcctttgc agctgatgtc gatgtcaatc
     1501 atgccgtggc gggcgatttg atcgatcatg tgttcgagga agggtacgcc ggtatccagc
     1561 ctgcttttgc cgctgccgtc gaggttgatg gagacggtga tttgggtttc gcaggtattg
     1621 cggttgacgg taacgctgcg gcagccggca gcagatgcgg tttcggcaat ttgggtttcg
     1681 gcagttccgg tgctttctgc ggcggtttcg gggacagtgc gttcgcggtg ttttctgtcg
     1741 agccagcctt tgggtttgcc ggtgtgtttt tcgagttttg ccatcaggct gggacggatg
     1801 ccgcgtgcat ctgggtcttc tgcctgcttt tcaaggcgtt gtttgagttt gtagagtgcg
     1861 acgggggtac ggtagccgca gagtttggcg agcttggaca gcgaacctgc ttcttgggcg
     1921 agggtcagaa agttgtgcag ttggcgttgt gttttagtca aattcatagg aaatccgttg
     1981 gttggcagta aagttggcga atgatgttga gaacggcatc gttttgtgcg gggctgccta
     2041 cggtaatgcg caggcagtgt tccaaaagcg ggtgcgcgcc atgcagtttt ttaaccaaga
     2101 tgcggttttg tttgagcgtg tcaaacaaca aatcggcatc gggtacgcgt atggtaatga
     2161 agtttgcctg acttggaaag gtgttcagac ggcatatttt gcccaattcg gcgaacatcc
     2221 gttcgcgttc gtttttcagg ctgtcgatgt tggcagagat gatgccgtag tgttgcaggg
     2281 cgagtttggc ggtggtcagg ctcagttggt tcatattgta gggcggcagg attttttgca
     2341 gttcgccgat gacttcggga cagcctgccg catagccgat acgcagtccg gcaaaaccga
     2401 ttttgctgac ggtacgcatg acgatgaggt tgggaatgct gcccgcctgc ggcaggaagc
     2461 tgtcgccgtt gaatgcgccg taggcttcgt cgacgacgac gatgccgtct gaagcttcga
     2521 tgacggcttc gatttcggca cgcgtgaaac atacgccggt ggggttgttg gggtaggcga
     2581 taaaggtcag ggcagggcgg tgcttcctga cggcttcgag gacggcgggc aggttgaggg
     2641 tgaaatctcc gttcagtgga acgccgacat aatccatgcc gtacagcgcg gcgttgtggc
     2701 ggtacatgac gaaactgggt tcggctgcca acattgccgc gcccggtttg gcggtcagca
     2761 tcgtgatgaa ctgtatcagc tcgtccgagc cgttgcccag cgcgatgtcg gcgcagtcgg
     2821 gaatgtcgaa cgccgaacgt aatgcttcct gtaaaccgct gccggaggga ttggggtaaa
     2881 gatggatggg cgcggcggca agccgtgcct gccattcctg caacagcgtt tcatgtccgg
     2941 caaaagggtg gacgggactt tccatcgaat cgagtttggc aaagccgggc ggaacgtcgg
     3001 caatctgata tgccgacata gcttgtatgt cgtcgcggat gaaggagcgg acggatttca
     3061 tcgtgtttcc ttaatggttg gaatatgcct gtacgccgtt tcggcattat ttcatacgga
     3121 actctgccgc gcgggcgtgg gcggtcaggc tttcgccgtg tgccagcacg ctggcggttt
     3181 cgcctaattt ttgcgcgccc tgttccgaaa cctgaatcag gctggagcgt ttttggaaat
     3241 catatgtccc caaaggcgag gaaaagcggg cggttcggct ggtgggcaat acatggtttg
     3301 gaccggcgca gtagtcgccg aggctttcgc cggtgtagcg tcccatgaaa atcgcaccgg
     3361 cgtggcggat ttttttcgcc cattcctgcg gattttcgac tgacagttcc aagtgttcgg
     3421 gggaaatgta gttggcgatt tcgcaggctt cgtccaagtc tttggcgagt atcatcgcgc
     3481 ccctgttgcc gagcgaggct tcgatgatgt cgcggcgcgg catagtttcg atcaggcggt
     3541 ccatagcggc ttctacttcg tcgagatacg cttgcgacgt gccgatgagg atggcttggg
     3601 caatttcgtc gtgttcggcc tggctgaaca aatccatcgc cacccaatcg gcaggtgtcg
     3661 tgccgtcggc gatgaccagg atttcagacg gccccgccac catgtcgatg ccgaccacgc
     3721 cgaacacgcg gcgtttggcg gcggcgacga aggcgttgcc cggaccggtg attttatcga
     3781 cttgggggat ggtttccgtg ccgtaggcga gggcggcaac cgcctgcgcg ccgccgacgg
     3841 tgaagacttt ggttacgccg gcgacgtatg cggcggcaag tacgatgtcg ttgcgttcgc
     3901 cttttggtgt cggcacgacc atgatgattt ctttcacacc tgcgacgtgg gcgggcatgg
     3961 cgttcatgat gacggaactc ggatacgccg ccttgccgcc ggggacgtaa atgccgacgc
     4021 ggtcaagcgg tgtgatttgt tgtcccaaca gcgtgccgtc ttcatcggtg tagctccacg
     4081 attccatttt ttggcgttgg tggtagcttt cgacacggcg ggcggcggtc tgcaatgccg
     4141 tctgaacgtc gttcggaatg cgctcgaacg ccgcgttcaa atcggcttgc gtgagtatta
     4201 aatcatcgat gcttttagcg tttgtctgat cgaatttgtt ggtgtattca atcaaagccg
     4261 catcgccgcg cttttgcacg tcggcgcaaa tgtcggcgac gatgcgttcg gtttcggggt
     4321 tttgcgcggt ttcaaaagcc agcagggctt tgagtccggc ttggaaatcg ggcgattggg
     4381 tgttgagttt tttcatgatt tggttttttc cttttttatt tttggattgg gggtcgtctg
     4441 aatattctct tgttcagttt ctgtctctgt tttgggtctg atatacgctt tttctccccc
     4501 gctaccaaac ttcaattcgg gcaggcattt ttgttgcaga ccgagtttgg ctaatacctc
     4561 ttcgctacga gaggtagtgc ggaaacggta acgggattgt tttctaccgt ttttggtttc
     4621 ctcaaccatt gtttcaaaac cgtccacttc gtatgcactg acaacagcgt tttgcagacc
     4681 ggtgtgaacg ccgataacgt atttcacttt tgaagcaaca tctttaccga taacccagtt
     4741 gcccaacgta cgcgatttga ggttggcatc gtcttggttg tcgaaaaggt agtctaattc
     4801 ttcgtcagta tctaaatcga aagcattgtg gattttgatg gcgagaatca gcccgtcggg
     4861 gttaatctcg ttaagtggga gagaagagaa tccaaagcgg cgatctagtt cttctacgct
     4921 aataccaccc ggaccatgcc cggcaatttt atttttgagt tttttaccca agacagattt
     4981 aacaaaatga attaaggcag attcggcggc gagtgcttcg actttagtca gatgatagct
     5041 gatgatatag cgaccgagtt tcttgcattt ggagatggct ttgagtttcc gatcgataat
     5101 ctcgccggag actgggtctt gtgaacgact agcaacccat tcatgctcga agatacgatt
     5161 accgcagcct ttgccgatgt agaggatttt gtcttttttc aggtcggtca gacaatagac
     5221 ataaaaccga cgctcgccgt tattcaaaac ggaaagggtt gaatctgaga atttttttat
     5281 ttttgcaacc attttaactt ctcctataca gtttactgcc ggtgatattt tcagacgacc
     5341 tgtcggcggg aacggatagt gtcgtctgaa aacgcatctt tattcaaatg gatgcttact
     5401 tcgccttcac tgcgccgccg aacgcctgaa taatcggctc cagcagcgcg tatttcgttt
     5461 tcaaagcagc cttgttgacc accaggcggc tggaaatgtc gacgatgtgt tcgactgcct
     5521 ccaagccgtt tgccttcaag gtgttgcccg tcgaaaccaa gtccacaatc gcatcgctca
     5581 agccgaccag cggcgcaagt tccatcgagc cgtacagttt gataatgtcc acatggacac
     5641 ccttgccagc aaaatgagat gccgcgattt caggatactt tgtggcaatc ttcagacggc
     5701 atccgggttg cgaagctgct tcgtaatcaa accctttacg cacagcaacc atcatgcggc
     5761 acttggcaat ctccaaatcc aaaggccggt aaagccccgt gccgccgtgt tcgatcagca
     5821 cgtctttgcc cgcaatgccg aagtccgccg cgccgtagcg gacataagtc ggcacatcgg
     5881 ttgcgcggac aatgacaagg cggatgtttt catggttcgt cccgataatc agcttgcgcg
     5941 atttttcagg ctcttcagtc ggaacaatgc cggcagcggc aagcagcggc agcgtctcct
     6001 caaaaatgcg ccccttggat aaggcgatgg tcaaagcatt atcctgcatg gtgtggatgt
     6061 cctttgtcgg ttgaaacaat aatgccgtct gaataggcgc cgacggtgca gacggcatac
     6121 ggttttatca gagcagggtc cttacatcgg cagcaatcgt ttccggctcg cttccgtaag
     6181 gcgagaaaat ggcaacctca ccgtttttgt cgatgagata cgcaccggaa gagtggtcga
     6241 ccaaatagtt ttcgctgtcg tctttttgat tgactttggc agaaaccacg cggtattgct
     6301 gcttgatgac cggcaggttt tggccgccca ttgccgtcag accgataaag tccggattga
     6361 actgtttggc atacttgccg atgatttcag gcgtgtcgcg ttccggatcg atgctgacga
     6421 acaccacttt cacgtcctta gcctgcccgc ccaactgctt caacgtgtcg ctgtacgtca
     6481 aaagctctgt cgggcagaca tcgggacagt gcgtaaagcc gaaagacaga atcacgacct
     6541 tgcctttcaa atcgctcagg ttgaaaggct tgccttcgcc gtcggtcagc gtgaaatcgc
     6601 cgccgatgtc ttccttacgc atatccgtac cgcgcgtttg cggctttgcc gcattttccg
     6661 cagccgacgc ggatgcactt gaagaagcga cttgcgccgc actgttgtct tgaggtttgc
     6721 aggcggcaag cgcggcaagt acgaaaacgc ccggcaaaaa ggaacgcggt acggaaaaca
     6781 tgacaatctc ccaagaataa atagcaaaac ctgattcgta cggaatttga atatttccat
     6841 cttaacgcgt ttggcagatt taatgtagta tttttttacg ttgtctgcac ggcaggaatt
     6901 gccgaatcat acagaatctt tggagcggaa agaacagtaa gattaaaata ataaaaattc
     6961 tatttaaaat caaaaataaa ataaaaacag ataaaataat ttcaaaaaaa aggtttacag
     7021 aatttcaaaa gccattataa tgacggcttc ttcaccagcc caggtggcga aattggtaga
     7081 cgcaggggac tcaaaatccc ccgccgcaag gtgtgtcggt tcgagtccga ccctgggcac
     7141 cacaaccgct tttagagcgg ttatttttct gtctattgca gtctaacgcc gtaaaccaaa
     7201 gccaagaaag acaaggtttt ccgctggttt cagtgcatat atatcatcta atcccgtcta
     7261 atctgttcta attgagttaa cctaaaattt acggtatttt ttacggtatc tgaaaaatac
     7321 ctcgaaaaaa taccgagacc tttgcaaaat ccccaaaatc ccctaaattc ccaccatgac
     7381 atttagggga ttttccatga gcaccttctt ccagcaaacc gcccaagcca tgattgctaa
     7441 acacatcgac cggttcccac tattgaagtt ggatcaagtg attgattggc agccgatcga
     7501 acaatacctg aaccgtcaaa gaacccgtta cct
//
LOCUS       DAXHKX010000093         7383 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHKX010000093 DAXHKX010000000
VERSION     DAXHKX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.093"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            423..2573
                     /gene="dinG"
                     /locus_tag="ACFMWZ_001410"
     CDS             423..2573
                     /gene="dinG"
                     /locus_tag="ACFMWZ_001410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC5935362.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRA
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            2610..3167
                     /locus_tag="ACFMWZ_001411"
     CDS             2610..3167
                     /locus_tag="ACFMWZ_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFC5935363.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYNRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLS"
     gene            complement(3319..5187)
                     /locus_tag="ACFMWZ_001412"
     CDS             complement(3319..5187)
                     /locus_tag="ACFMWZ_001412"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC5935364.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(5267..6637)
                     /gene="purB"
                     /locus_tag="ACFMWZ_001413"
     CDS             complement(5267..6637)
                     /gene="purB"
                     /locus_tag="ACFMWZ_001413"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC5935365.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAGTLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSSVPEVAAASEFIHFACTSEDINNLSHALMLQEAREVILLPKLAEIIEKLTGMAHEL
                     AAVPMMSRTHGQPATPSTLGKEIANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEIGLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(6709..7341)
                     /locus_tag="ACFMWZ_001414"
     CDS             complement(6709..7341)
                     /locus_tag="ACFMWZ_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC5935366.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAAFFSYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLVRGLISEGAVSLFAISDDEDEIVAYLSENGLQTA"
BASE COUNT         1834 a         2132 c         1895 g         1522 t
ORIGIN      
        1 gcacatcctg tcgccggtat tccctaagcg gtacggcaac ctaaaacaaa tgccgtctga
       61 acttccgttt cagacggcat ttttatacgg actactcaat atcttccgtt tttttcactt
      121 tcaataatca gatatgccca aatcaccgcc acccaagccg acaccgttcc catcgccggc
      181 aaaaaatgct gccaatccgt catcggcggc acagggcaac ggccgcattc gatttccgcg
      241 ccgcccaaca accggtacag caacatatcc gcaatccaaa gcaacaacgg gtatttcaaa
      301 cgctgcaacc aaaaaaccaa ccacgtccgc acgattcacc tcacccccca ttccgacaac
      361 gatattgtac cgcttccgtg caatgttaaa ataagcccac ttctaccgcc gtacaaaacg
      421 ccatgctcac cgatttagaa aaaaacgcca tccgcgacca ttaccaaaac atcggcaaaa
      481 acctgcccgg tttccgtccg cgcgcttcgc agcgggaaat gattgcggcg gttgccaacg
      541 ctttttcgcg gacgttggcg cgcgaagaag gcggcgagcc gcccaagcgc gagggcgaga
      601 gcatcgccgt aatcgaaggg ccgaccggcg tgggcaaatc gcttgcctac ctgctggcgg
      661 gcggcatcat ggcgcaaaca cgcggcaagc ggctgattgt gagcagcgcg acggttgcct
      721 tgcaggagca gttggtagac cgcgacctgc cgtttctggt cgaaaaaagc ggtttggaac
      781 tgaccttcgc acttgccaaa gggcgcggcc gctatctctg cccctacaaa ctctatcaac
      841 tgacgcaaag caatgcccag caaaacctgc tcggctttga agcccccgcc gtcttgtggg
      901 acagcaaacc caagcccgaa gaattgaagc tgctgcgcga cattgccgac gaattttctg
      961 cccgacggtt caatggcgac cgcgacgcct ggccggaaaa aatcgacgac gcgatttggc
     1021 tcaaagtgac caacgaccgc cacggctgcc tcaaagccgc ctgtcccaac cgcgccgaat
     1081 gcccgtttta cctggcacgc gatgtcttgg aaaccgtcga tgtcgtcgtt gccaaccacg
     1141 accttctgct tgccgacatc agtatgggcg gcggcgtgat tctgcccgcg cccgaaaaca
     1201 gtttctattg catagacgaa gcgcaccacc tgcccaaaaa agccctcagc cgttttgccg
     1261 ccgaacattc gtggaatatt gccgtttgga cgctggaaaa actgccgcag ctgaccggca
     1321 aaattgccgc gctgaccgat aaagccgaac ttgccaacct tgccgacgaa gccgccgcat
     1381 ccttgctcga cagcctgcac gaatggcagt tccatttggc ggaagagccg tctttaagtt
     1441 tgggggtgtc tgaaaacgac agacgaacca acagcgaacc gacttggctg tgggaagacg
     1501 gcaaaatccc cgaaggcctc gaaaccaccg tttccaatac ggccattgct gcgcgcagcc
     1561 tgctcaaaca cgttgtcggg ctgaacgatg cgctttccgc cgcgcgccgc gaaaaagaac
     1621 aggacggcgc gctcctcgac cgcctgaccg gcgagttcgg catcttcatc gtccgtatcg
     1681 aacaaatcag cgcggtttgg gatttgctct ccaccgtccc cctcgaaggc gaagagccgc
     1741 tggcaaaatg gataacccgc cgtgccgacg acaaaaacga ctacattttc aacgccagcc
     1801 ccatcagcag cgcatcccac cttgccaaca gcctgtggcg gcgtgcggca ggcgcggtat
     1861 tgacttccgc caccctgcaa tccttgggca acttcaacct gatgctgcgc caaaccgggc
     1921 tgcaatggct gcccgaaacc accaccctcg ccctcaaaag cccctttgac tttgaaaaac
     1981 agggcgaact ctacatcccc cccatatacg ccagccccaa agaccccgaa gcccacaccg
     2041 ccgccgtcat cgaatggctg cccaagctta tttcgcccac cgaagccatc ggcacgctcg
     2101 tcttgttttc ctcgcgcaaa caaatgcagg atgtcgccct gcgcctgccc ggagactacc
     2161 tgccgctctt gctcgtacaa ggcgaattac ccaaagccgt cctcctgcaa aaacaccacc
     2221 gggccataga agaaggcaaa gccagcatca tcttcggact cgacagtttc gccgaaggac
     2281 tcgacctgcc cggcaccgcc tgcgtgcaag tcatcatcgc caagctgccg tttgcgatgc
     2341 ctgacaaccc catcgaaaaa acccaaaacc gctggataga acagcgcggc ggcaatcctt
     2401 ttattgaaat caccgtcccc gaagccggca tcaaactcat ccaggccgtc ggccgcctca
     2461 tccgcaccga acaagactac ggccgcgtaa ccatcctcga caaccgcatc aaaacacagc
     2521 ggtacggcca acaattattg gccggcctgc cgccgtttaa aaggataggg taaacatacc
     2581 gcccttcccg caacagaaag gaaaaattca tgaactttac ccgactgctc aaccaagtct
     2641 taagcacggt tcaaaaaaaa ggcaacacat tctccgacag cccgctcaat tcattcggcg
     2701 gaggcgcgct ggttgccggt gtcgcctcca tgctgctgaa cggtaaaaac cgcaaaacca
     2761 tcaccaaaat cggttcgacc gccgctttgg gctacctcgc ctaccggggc tatcagatgt
     2821 ggcagcaaaa caaagggcgg gcaaccgtaa cacaaagcga tttccaacct gccggagaaa
     2881 ccgaagaaac atacaaccgc accgtattgc gcaccatgat agccgccgcc gcttcagacg
     2941 gcatgataga cgaagccgaa cgccggacta tcgaacagga aagcggcaca gaccccgaaa
     3001 ctgctgcatg gctcgccgcc gaataccgcc tgcccgcaag catcgaagac atcgccgccg
     3061 ccgtcggcaa cgatgaggcg ttggcggcgg aggcctatct ggcggcaaga ctggtatgtg
     3121 ccgatttgtc gcggaaagaa accgtcttcc tcgcccgcct gtcgtaggct ttggaactgg
     3181 atgacaatct ggtggaaaac ttggaaaggc aattggggtt ttgattcaaa attccctccc
     3241 gcaggggaag gaaaataaaa accgccgccg ccccgaaagg caaacggcgg cacaaacgat
     3301 atcggacggc aaggcaggct actgcaatgc gccgtcgcga gaagatttaa gcaggcgttc
     3361 gtgtttgtga aattcccgcc ccatataata agaaccgaaa taggtcaggt ggtcttggtc
     3421 gccgtaaaga tagcggccgt atatttcgac cgtgtttttg ggcaggtatt tttgtgcgtc
     3481 cacccaatgc acattgggaa tatctttaat caaatcaaag accgcctgat tgctcttgcc
     3541 gatgtcgccc atagcctgaa tggggcggag atattggttt gcggcaaatc ttttcaattt
     3601 ttcctccctc aggggcgaac ggctgattga tgtgttgttt gcaaaaacat agacgggttt
     3661 gacggcggct atccttttga cggtttccct gaatcgggct gggaacccgg gtattaggaa
     3721 ggattgcgct tcaaatctcg gcacgggctg gccgcccatc ctcaaatcat agaattgggc
     3781 aatgaaaacg gcttcggctt tttcaacttc atcccggtat tttcgacata acgggttgtc
     3841 tgccagcttc tcatctaccc aaaccaaaca ctccgaatcg agggacagga ttttggcttt
     3901 ccacccttcc cggctgccga cataatccag aaacccccgc aggtgtccgg cgtgcgagtc
     3961 gccgagggtc aggacggttt ccggaaaatg attttccgca gcaaggggcg cgccgggcaa
     4021 cgggcggagg tgttcctgtt tcaatatccc ccttgcgtac aggttgtaac cgacaagtat
     4081 cagggacggg gcgagataga ggcagaaaaa tgcctttttg aaggtcatct tccgttttct
     4141 aagcggctgt tcaatcaaat aataactcaa cagggaaaat ccggccgtca acgcggcaac
     4201 cgccgatacg gcaggcagtc cgagctgttt gtcgcctgta atgtaatggg cgaaagcaat
     4261 aaaaatccaa tggtacaggt atagggaata agagattttg ccgacaaata cgatggggct
     4321 tgccgacagg atgcgggtcg gaagtgtccc gtattgcata ctccggataa gcagtgccgt
     4381 cagcaggcag ggaaggagca gggtcattcc cgggataaac ggattgtgtt tgtcaatcac
     4441 gaacaggcag gcaagcaatg cgccgaagca gagtgatgaa agcaactgcc gttttccatt
     4501 tgctgtttgc cgtctgccgt tttgcgtttg cccgtaaacc gccagcagcg aacctgccaa
     4561 cagctcggga aacctcagtg tcgaaaggta ataagtattg ggttggttga gaatatcggt
     4621 ataaaacccg cttggcaaaa acgatgtggc agtcaaaatc agaaatagga tgatgctgat
     4681 gttacgcagc acccgtagcg attttgtttt tttgcagcaa aatatcagca aaagaggata
     4741 caggaggtaa tactgttcct ctactgccaa agaccagata tgcagtacgg ggttctcgtc
     4801 ggcactcaaa tcgaaatacc cctgctgaaa ccccagataa atattggaca agaaaaccgc
     4861 agaaagctcc acggttttcc gcatttggtt gaaatcttcg taaaggaaga tttgagaggc
     4921 aatcaccgaa gccagcgaca cggccgcaat aaaggcaggg taaatccgct taatcctgcg
     4981 ggtataaaaa tcccggaaag aaaaagaacc gttctgtatt tcagaaagaa tgatgccggt
     5041 aatgaggaat cccgagatga caaagaaaat gtccaccccc aaaaaccctc cgggcagcca
     5101 gcggttattc aggtggaaaa taatgacgga tagcacggca acggcccgca atccgtcaat
     5161 ttcaggcctg tatcggacag cttgcataaa tatcgccccg tatgtcccgt tatcttaaaa
     5221 atgccgtctg aacgcgcgtt cagacggcat cggtttcagt aaacgctcaa atccgtttcg
     5281 ccaacgcttc agccttgccc acatacagtg cgggggtcag ctcaagcaat tttgctttgg
     5341 cttcgacggg gatttccagc gatccgataa agcctttcag cacttcgggc gtaatgccgc
     5401 ctttgccgcg cgtcaggtct ttcagttttt cgtaaggatt ggcgacaccg taacggcgca
     5461 tcacggtttg aatcggctcg gcgagcagtt cccaagtggc atccaaatcg gcggcaagcg
     5521 cggcagggtt gggttcgagc ttgttcagac cgcgcaggtg ggcggcgaaa cccaacacgg
     5581 catagcccac gcccacgccc atattgcgta agacggtgct gtcggtcagg tcgcgctgcc
     5641 agcgggaaat cggcagtttt tcggacaaaa agcccaatac ggcgtttgcc ataccgaggt
     5701 ttccttcgga gttttcaaag tcgatggggt tgactttgtg cggcatggtg gaagaaccga
     5761 cttcgcccgc tttgactttt tgtttgaagt aacccaatga aatataaccc caaacgtcgc
     5821 ggttaaagtc gatcagaatc gtgttgatgc ggctgagggt ttggaagaat tccgccatgt
     5881 agtcgtgcgg ctcgatttgg atggtgtagg ggttgaaggt cagaccgagg ccgatttcga
     5941 cgaagttacg gcaatgggtt tcccaatcca catcgggata agcgaccata tgggcattgt
     6001 agttgccgac tgcgccattg attttgccga ggaactcttg cgcttgcagg tttttaaact
     6061 gacgttgcag gcggtacacg acattggcga tttctttacc cagtgtgctt ggtgtagcag
     6121 gctggccatg cgtacggctc atcataggga cggcagccaa ctcatgcgcc atgccggtca
     6181 gtttttcgat gatttcagcc agtttcggca gcaaaataac ctcacgcgct tcttgcagca
     6241 ttaaggcgtg ggacaggttg ttgatgtctt cgctggtgca ggcaaagtgg atgaactcgc
     6301 tggcagcagc cacttcaggc acgctggaga agcgttcttt aagccaatat tcaatggctt
     6361 tgacatcatg attggtcgtg gcttcgatgg ctttgacggc ggccgcgtct tccaatgaaa
     6421 agttttcaat caccgtgtcg atttcggcaa gcgttccggc actgaagggc ggcacttcga
     6481 caatcttcgg ctcggcggcc agggctttga gccagtttaa ttcgactttg acgcgcgcct
     6541 tcatcaggcc gtactctgaa aaaatcgggc gcaatgcttc aacggattgg gcataacggc
     6601 cgtctaaagg ggaaagcgag gcgatggggt tgatcatttc ggcatcctgt tcgggaaaga
     6661 catcaaaaat tgttaaattg tttgcaatta tacatcactc tcaggacgct atgccgtctg
     6721 aagcccgttt tccgacagat acgcaacgat ttcatcttca tcgtccgata tggcaaacaa
     6781 agagaccgcc ccttcggaaa tcagaccgcg caccaaaagc tgcgcgttta tccactccgc
     6841 caagcccgac caaaatgcct tcccgaccaa aacgaccgga cgcggcggta ctttgcccgt
     6901 ctgcaccaag gttaggattt caaacaattc gtccagcgtc ccgaagccgc ccggcatcac
     6961 gacatatgct tgggaatagc tgaaaaacgc cgccttgcgt tcggcaaaac gggaaaaccg
     7021 caaggcgatg tcctgatacg gattcggttt ctgctcgtgc ggcaaaacga tgttcagccc
     7081 caccgaaacc gacttccctg caaacgcgcc cttgtttgcc gcctccataa tccccggccc
     7141 gccgcccgaa atgacggcaa tgcccgaatc cgacagccgc cgcgccagac ggcaggcgaa
     7201 cgcataatcc gcatgattct gcggcgtgcg cgcgctgccg aagatactga ctgccggaaa
     7261 cacgcccgcc aatgcttcgt ctgcctgcct gcgttcggca tcataacgtg cctgctccgg
     7321 cacacggttt gtattctcca ttccatcctc cgttcaaaaa cagcgattgt acaccgtcaa
     7381 aaa
//
LOCUS       DAXHKX010000094         7372 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHKX010000094 DAXHKX010000000
VERSION     DAXHKX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.094"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..327
                     /locus_tag="ACFMWZ_001415"
     CDS             <1..327
                     /locus_tag="ACFMWZ_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC5935367.1"
                     /translation="TEEGIAQTVVKGKTTRAEVEARFGKRNPFGCYAYHEVSLPIYNF
                     LPTNFIYMKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVIRKEAGKS
                     LSQPEK"
     gene            391..1674
                     /gene="hemL"
                     /locus_tag="ACFMWZ_001416"
     CDS             391..1674
                     /gene="hemL"
                     /locus_tag="ACFMWZ_001416"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC5935368.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(1890..2162)
                     /locus_tag="ACFMWZ_001417"
     CDS             complement(1890..2162)
                     /locus_tag="ACFMWZ_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HFC5935369.1"
                     /translation="MPSETQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2173..2736)
                     /gene="orn"
                     /locus_tag="ACFMWZ_001418"
     CDS             complement(2173..2736)
                     /gene="orn"
                     /locus_tag="ACFMWZ_001418"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC5935370.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2754..3641)
                     /gene="prmA"
                     /locus_tag="ACFMWZ_001419"
     CDS             complement(2754..3641)
                     /gene="prmA"
                     /locus_tag="ACFMWZ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC5935371.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            complement(3759..5120)
                     /gene="accC"
                     /locus_tag="ACFMWZ_001420"
     CDS             complement(3759..5120)
                     /gene="accC"
                     /locus_tag="ACFMWZ_001420"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC5935372.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEEERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5235..5690)
                     /gene="accB"
                     /locus_tag="ACFMWZ_001421"
     CDS             complement(5235..5690)
                     /gene="accB"
                     /locus_tag="ACFMWZ_001421"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC5935373.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKVGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5672..5929)
                     /locus_tag="ACFMWZ_001422"
     CDS             complement(5672..5929)
                     /locus_tag="ACFMWZ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC5935374.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            6046..7086
                     /gene="queA"
                     /locus_tag="ACFMWZ_001423"
     CDS             6046..7086
                     /gene="queA"
                     /locus_tag="ACFMWZ_001423"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC5935375.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1521 a         1860 c         2218 g         1773 t
ORIGIN      
        1 acggaagagg gaatcgcaca aacggttgtt aaaggcaaaa cgacccgggc ggaggtagag
       61 gcacgtttcg ggaagcgcaa ccctttcggt tgttatgcct atcacgaggt cagcctgccg
      121 atttataatt ttttgccgac caatttcatt tatatgaaat cagaacgccg acattgggag
      181 tggtgtgtgg attacgactg ggaaggagtt gtccgggact accgctttac acataaagtg
      241 gaaaaagacg agcgttccgt catccgggat gcggttggcg tcatccgcaa agaagcaggc
      301 aaatccttgt cgcaacctga aaaatgataa aatgggactt tctgcttcta agcccgaaac
      361 ctgccgttca ggcggcattt gaggataaat atgaaccgta acgaaatttt attcgaccgc
      421 gccaaggcgg tcatccccgg cggcgtgaat tcccccgtcc gcgcattcgg cagcgtcggc
      481 ggcgtgccgc gcttcatcaa aaaagccgaa ggcgcgtatg tttgggacga aaacggcacg
      541 cgttataccg actatgtcgg ctcttggggg cctgcgattg tcggacacgc gcaccccgaa
      601 gtcgtcgaag ccgtgcgcga agctgccttg ggcggtttgt cgttcggcgc gcccaccgag
      661 ggcgaaatcg ccattgccga acaaatcgcc aaaatcatgc cgtccgtcga acggctgcgc
      721 ctcgtcagct ccggtaccga agcgacgatg accgccatcc gtttggcacg cggttttacc
      781 ggtcgcgaca aaatcatcaa gtttgaaggc tgctaccacg ggcattccga cagcctgttg
      841 gtgaaagcag gcagcggtct gctcaccttc ggtaatccct cttccgccgg tgtgcctgcc
      901 gactttacca aacacacttt agtcctcgaa tacaacaaca tcgcccaact cgaagaagcc
      961 tttgctcaaa gcggcaatga cattgcctgt gtgattttgg agccttttgt cggcaatatg
     1021 aatctggtta ggccgtctga agccttcgtc aaagccctgc gcgaattgac cgaaaaacac
     1081 ggcgcagtgt tgatttacga cgaagtgatg accggtttcc gcgtcgcgct cggcggcgca
     1141 caatcgctgc acggcatcac gcccgacctg accacgatgg gcaaagtcat cggcggcggt
     1201 atgccacttg ccgcgttcgg cggacgcaaa gacattatgg aatgtatttc cccgctgggc
     1261 ggcgtgtatc aggcaggcac cttgtcgggc aacccgattg ccgtcgccgc cggtttgaaa
     1321 acgctggaaa tcatccgccg cgaagggttt tatgaaaacc tgacggcgcg taccgaacag
     1381 ttggttcaag gttttcggac ggcagcggat gcggcgggca tcgagttcac cgccgacagc
     1441 gtgggcggta tgttcggtct gtatttcgcc gcacacgcgc cgcgaaatta tgccgatatg
     1501 gcgcgttcca atattgaagg cttcaaacag tttttccacg ggatgctcga ccgcaatgtc
     1561 gccttcggcc cgtccgctta tgaagcaggt ttcgtttctg ccgcgcatac gcccgagctg
     1621 attgacgaaa cggttgcggt tgcggttgaa gtgttcaagg cgatggctga atgatgtttt
     1681 gacggacaga gtttctctgt tcgatttgtt tggcagattg aagtaagaat gcacaccgcc
     1741 gtcattcccg cgcaggcggg aatccagacc tttcagtttc tgtaatgatt gaaaataacg
     1801 ggccgaaatc agccgcgccc ggcaggaagc cgccgccacc tgaaacaatg ccgtctgaag
     1861 ccttcagacg gcattttatc ggaagcgcat cagaaaaaac ggatagcgac atatgcggta
     1921 aaagcaaggg caattacata gataaacccc tttataaatt caaaagtctc atctatatcg
     1981 ctatattttt ctttcagttc gtcagatttt cgataacgtt tcattttcaa gaccacttca
     2041 tgaccaacaa gcccgacctt ccggattccc gcctgcgcgg gaatgacggg cgtgtatatt
     2101 tttgatttca atctgctgta aaaatgccgt ctgaaatgca ggatttgcgt ttcagacggc
     2161 attgtttgtt tcttattgcg cttcggctct cggggcggaa atcagaaagt gttcgcggta
     2221 gtggcgcatt tcttcgatgc tctccaaaat gtcgtccaat gccttgtgcg aaccgcgttt
     2281 gacgacgctt ttggcaacgg gcggattcca gcgtttggca agctctttca gtgtggaaac
     2341 gtcaaggttg cggtagtgga agtagttttc cagtttcggc atatatttga ccataaaacg
     2401 ccggtcttgg tggatggaat tgccgcacat cggcgtggcg cgtccgggta cccattccga
     2461 cataaagtcc agcagtttct gttcgacttc ggcttcggta tgcgacgatt cgcgtacgcg
     2521 ctgtgtcagc cccgtcctgc cgtgtgtggc ggtgttccac tcgtccatat tgtcgagcag
     2581 ctcgtcactt tggtggacgg cgtaaacttc ggattgcgcc aatacattca aatccgagtc
     2641 ggtaataatc atcgcgactt cgataatgcg gtcggtttcg ggattaagcc ccgtcatttc
     2701 catatcgagc cagcagaggt tgtttttgtc ctgcacggtg tttcctttcc gtttcagcgt
     2761 tttgtcccgc tcaagcgcgc ccagccttct tcggtttccg ccgggtcgag atcgaaccat
     2821 tggctgtaaa tgccgccgag ttcttcggcc tgttcgtcca acaaaccgga caacacgatg
     2881 cggccgcctt gtttggtgcg ggcggcgagc atttcgccga gcatacgcaa agggttggcg
     2941 aggatgttgg cgacaactac gtcgaattgc ccttgaggca gaccgtcggg caggaagaat
     3001 tgtgcatcga cgttgttctg ttcggcgttg tccctgccgg cgcggacggc ttgttcgtca
     3061 atatcgacgc cgacggcaga acctgcgccg agtttgaggg cggcgatggt caggatgccc
     3121 gaaccgcagc cgtagtcgag gacgctttcg ccgtttttga gttgcgtatc caaccatttg
     3181 aggcagaggc gcgtggtcgg gtggctgccg gtgccgaagg cgagtccggg gtcgaggcgg
     3241 aggttgacgg cgctgccttc ggggacttcg tgccaagagg gggtaatcca caggcggtcg
     3301 gaaatccgga tggggtcgaa ttgcgattgc gtgagacgca cccagtcttg gtcttcgatg
     3361 gtttcgccgg tgtatgccaa gtcttttaac ccgcattctt gtgcggcgat ttggatgatg
     3421 gcggcggctt cgtcgtgttc gccgaacagg gcgatgactt tgctctgctg ccagatttgt
     3481 tcggcgggca taccgggttc gccgaaaatc gcctgttcgt tttgcgtacc ggcgtaggca
     3541 tcttcgatgg cggcggagag tgcgccgtgt tccatcagcg cgtcggcgag gcgttcggcg
     3601 acggcatcgt tgacgttgac ggtgatttgt tggtagggca tagcgggctt tcttaacaga
     3661 ccttcggaat agggtcgtac tgtataaggg gatgggtgtg taacggaaat gccgtctgaa
     3721 aacggacaaa ctgtttcaga cggcatatcg gggacggctt atttgtcctg tttggctttg
     3781 cgatcttcca gccagtgttc caaatagtgg atgctgacgc cgcctttttg gaaacccgca
     3841 tcggcgaaca ggtcgcggtg aagcggcgta ttggttttga tgccggttac cgccagctcg
     3901 gcgagtgcga cgcgcatttt cgccattgcc tgttcacgcg ttttgccgtg tacgcagatt
     3961 ttgccgatca ggctgtcgta gtacggtggg atgcggtagc cttggtaaat gtggctgtcc
     4021 acgcggatgc cgaagccgcc gggcaggtgg cagctttcaa tcggccccgg gctggggatg
     4081 aagttgtacg ggtcttcggc gttgatacgg cactcaaacg cgtggccttc gacttgaata
     4141 tccttttgtt tgtattgcaa cggcaggccg gcggcgatgc gcaattgctc ttgtacgatg
     4201 tccacgccgg tgatgagttc ggtaaccgga tgctcgacct gaacgcgcgt gttcatctcg
     4261 ataaagaaaa attcgccgtc ttcgtacagg aattcaaacg tacctgcgcc acgatagccg
     4321 atgcgtttgc aggcgtcggc acaggcgttg ccgattttgg cgcgttcttc ttcagtgatg
     4381 aacggagccg gtgcttcctc gatgactttt tggtggcggc gttgcagcga acagtcgcgc
     4441 tcggcaagat atacggcgtt gccgtgttcg tcggcaatca cttggatttc gacgtggcgc
     4501 ggacgttgca gatagcgttc catgtaaacc atcgggttgc cgaatgccgc gcctgcttct
     4561 gccttggtca tttcgacaga ttggaggagg tcttcttttt tctcgaccac gcgcataccg
     4621 cgaccgccgc cgccgccaga ggctttgata atgacgggat aaccgacttt atcggcgatt
     4681 ttgaggattt cttcgccgtc gtcgggcaat gcgccgtcag aaccggggac gcagggtacg
     4741 cctgccgcta tcatcgcgtg tttggcggag actttgtcgc ccatcaggcg gatggtgtcg
     4801 ggtttcgggc cgataaaggt aaagccggac tgctcgacct gttcggcgaa atcggcgttt
     4861 tcggcaagga aaccgtagcc cgggtggaca gcgtccgcgc agcttacttc ggcggcggcg
     4921 ataatggcgg ggacgttaag gtagctttgc gcggaagcgg cagggccgat gcacacggat
     4981 tcgtcggcga gtttgacgtg caggctgtct ttgtcggcct cggaatgcac ggcgacggtg
     5041 gcaatgccca tttcgcggca ggcacggagt acgcgtaatg cgatttcgcc tcggttggcg
     5101 attaaaactt ttttcagcat gatgactgtt cctgtggttc cggcaacccg tccgtaaaaa
     5161 aaaaaggtgc gggaacgccg aaaggggaaa gcatttcaga cggcatcgga agtttatgcc
     5221 gtctgaaaac aggattatcc gatgatgaag agcggttcgc cgaattcgac gggcgtaccg
     5281 ttttcgacca aaatttcttt gaccgtgccg gatttttcgg cttcgatttc gttcatcagc
     5341 ttcatcgctt cgatgatgca cagcgtgtcg ccgactttaa cctgttggcc gacttcgaca
     5401 aaaggcgcgg cattcgggcc gggtgcgcgg tagaacgtgc cgaccatagg tgatttttgc
     5461 gcgtcggaca aatcgcgggc ggcaggtgcg gcggcgggcg cggatgccgc aacgggtgcg
     5521 gcggcaggcg ttacggccgg cgcggcggca ggtacgggcg cggcgtaaac gggtgcggcg
     5581 gcgatggttc gggtgatgcg gactttttcc tcgccttcgg ttacttcgat ttcggcgata
     5641 cccgattctt caaccaaatc aatcagtttt tttaatttgc gcaaatccat ttctgttcct
     5701 ttaaaggctg ccggtgtttc ggcgggttgt tgcgttggtt tttcagaaaa cagccggctt
     5761 gcggcaacgg cgcaaaccgg gcggaatggt gttcggatac ggttttcctg tggtggaaaa
     5821 cgaccgtgag aatgtgttta ttttcccgaa gttgtcggta aatgtccagt aaaatatcaa
     5881 aaaacggcgg tttttggggg aaatgtgcag gaagcttgat tttgcgcaca agatgccccg
     5941 ggtcaagagt gtttattcta atctgttggt ttttcggaca aagatgccgt ctgaaaaagg
     6001 gctaaagtgc gtataatggc ggcttgcccg aacgagagtg taaaaatgga tatttcagat
     6061 tttgatttta ccctgcccga aaagctgatt gcccagcatc cgcccgaagt gcgcggcagc
     6121 agccgccttt tggttgcgtt gcccgatatg ccgctgcaag accgggtgtt cggcgatttg
     6181 ccggattatg tcgaggcggg cgacgttttg gtgttcaaca acaccaaagt catgaaggcg
     6241 cggctgtttg ggcagaaaga cagcggcggc aggatcgaag ccctgattga gcgcgtgttg
     6301 gacaaccata ccgcactggc gcacatccgt tcgtccaagt cccccaagcc cggtatgggg
     6361 ctggtgtttg aaggcggcat ccgtgccgtg atggtcgggc gtgagggcga actgttctgc
     6421 ctgcgttttg aaggtggaga gaccgtttac gaacttttgg aacagaacgg acacctgccc
     6481 ctgccgcctt atatcgaacg tgccgccgat gcggacgacg acagccgcta ccagactgtt
     6541 tatgccaaat atcagggcgc ggtcgccgcg ccgacggcgg gcctgcattt tacggaagaa
     6601 cttttgcgcc gtctgaaaga caaaggcgcg gtaaccgcag aagtaactct gcacgtcggt
     6661 gcggggacat tccaacccgt gcgtgtggac aaaatcgaag agcataaaat gcacagcgaa
     6721 tggtttgaag tgccgtctga aaccgccgcc gccgttgagg cggcaaaagc ccgggggaac
     6781 aaagtctggg cggtcggcac gacttccatg cgcgccctcg aatctgccgc gcgtgcgacg
     6841 ggtcgtctga aagcggggca gggcgacacc gatattttca tcacgccggg ctaccgtttt
     6901 aatgttgtcg acaggctggt taccaatttt catttgccga aatcgacgct gctgatgctt
     6961 gtcagcgcgt tttcgggtat gggtcatatc cgcgccgcgt atcgttatgc ggtcgaacgc
     7021 gaataccgtt tcttcagcta cggcgatgcg atggttttgg ggcggaacga aggggtggta
     7081 cgttagaaca gcctcaagtc tattggggga agacaagtgc cgtctgaacg gatgttcgga
     7141 cggcattctt ttgttgattt gaatgaaagg aactgcatga catatagtgg attaaattta
     7201 aatcaggaca aggcaacgaa gccgtagaca gtacaaatag tacggcaagg cgaggcaacg
     7261 ctgtactggt ttttgttaat ccactataga ttcaggcgcg agaggcgaga aactcagtca
     7321 ttgttgatga cgcgcaatac gttaaaattc attccgtttt ggtcgtataa ac
//
LOCUS       DAXHKX010000095         7358 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHKX010000095 DAXHKX010000000
VERSION     DAXHKX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.095"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     regulatory      76..120
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            125..598
                     /gene="queF"
                     /locus_tag="ACFMWZ_001424"
     CDS             125..598
                     /gene="queF"
                     /locus_tag="ACFMWZ_001424"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC5935376.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            673..1359
                     /locus_tag="ACFMWZ_001425"
     CDS             673..1359
                     /locus_tag="ACFMWZ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC5935377.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWVAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(1709..3001)
                     /locus_tag="ACFMWZ_001426"
     CDS             complement(1709..3001)
                     /locus_tag="ACFMWZ_001426"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC5935378.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            3079..4047
                     /locus_tag="ACFMWZ_001427"
     CDS             3079..4047
                     /locus_tag="ACFMWZ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935379.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(4248..5714)
                     /locus_tag="ACFMWZ_001428"
     CDS             complement(4248..5714)
                     /locus_tag="ACFMWZ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC5935380.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEAKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(5956..>7358)
                     /gene="autA"
                     /locus_tag="ACFMWZ_001429"
     CDS             complement(5956..>7358)
                     /gene="autA"
                     /locus_tag="ACFMWZ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autoaggregation autotransporter AutA"
                     /protein_id="HFC5935381.1"
                     /translation="NNIFNKKLHVKIENKSHVAGQVLELTKMTLKDSLWEPRRHSDIH
                     MLETSDNARIRLNTKDEKLTVHKAYQGGADFLFGYDVRESDEPALTFEDKVSGQSGVV
                     LERRPENLKTLDGRKLIAAEKADPNSFAFKQNYRQGLYELLLKQCEGGFCLGVQRLAI
                     PEAKAVLYAQQAYAANTLFGLRAADRGDDVYAADPSRQKLWLRFIGGRSHQNIRGGAA
                     ADGRRKGVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAAGSYLHGYGGGVY
                     AAWHQLRDKQTGAYLDGWLQYQRFKHRINDENRVERYKTKGWTASVEGGYNALVAEGV
                     VGKGNNVRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRAGIRAKTRFALRN
                     GVNLQPFAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSARIGYGK
                     RTDGDKEAALSLKWLF"
BASE COUNT         1530 a         2291 c         1856 g         1681 t
ORIGIN      
        1 acacggaaaa cgccgccgtc caaaccatgc cgtctgaaga aaactacaca gataccgccg
       61 cttatattac aatcgccgcc cgtggttcga aaacctccca cattaaaaaa ctaaggaaac
      121 cctcatgtcc cgcaacaacg aagagctgca aggcatctct cttttgggca atcaaaaaac
      181 ccaatatccg accggctacg cgcctgaaat tctcgaagca ttcgacaaca aacatcccga
      241 caacgactat ttcgtcaaat tcgtctgtcc cgagttcacc agcctctgcc ccatgaccgg
      301 gcagcccgac ttcgccacca tctacatccg ctacattccg cacatcaaaa tggtggaaag
      361 caaatccctg aaactctacc tcttcagctt ccgcaaccac ggcgattttc atgaagactg
      421 cgtcaacatc atcatgaaag acctcattgc cctgatggat ccgaaataca tcgaagtatt
      481 cggcgagttc acaccgcgcg gcggcatcgc cattcatcct ttcgccaatt acggcaaagc
      541 aggcaccgag tttgaagcat tggcgcgtaa acgcctgttt gagcacgacg cacaataaac
      601 catccttccc atatttcaga cggcattcct tcgatacgat atgccgtctg aacgcctttc
      661 cgaaagattt ttatgtacgc atttaccgac gcacagcaac agaaggcact cttctggctg
      721 gtgctttttc atatcctcat catcgccgcc agcaactatc tggtgcagtt ccccttccaa
      781 attttcggca tccacaccac ttggggcgcg ttttcctttc ccttcatctt cctcgccacc
      841 gacctgaccg tccgcatttt cggttcgcac ttggcacggc ggattatctt ttgggtcatg
      901 ttccccgccc ttttgctttc ctacgtcttt tccgttttgt tccacaacgg cagttggacg
      961 ggcttgggcg cgctgtccga attcaacacc tttgtcggac gcatcgcgct ggcaagtttt
     1021 gccgcctacg cgctcggaca aatccttgat atttttgtgt tcaacaaatt acgccgtctg
     1081 aaagcgtggt gggttgcccc gactgcatca accgtcatcg gcaacgcctt agatacgttg
     1141 gtatttttcg ccgttgcctt ctacgcaagc agcgatggat ttatggcggc aaactggcag
     1201 ggcatcgctt ttgtcgatta cctgttcaaa ctcaccgtct gcggtctgtt tttcctgccc
     1261 gcctacggcg tgattctgaa tctgctgacg aaaaaactga cgaccctgca aaccaaacag
     1321 gcgcaagacc gccccgcgcc ctcgctgcaa aatccgtaaa acagccctgc cggaaatcgg
     1381 gacggttggg ttttctttta tttgcaaaca ctgtcggaac ggataaatcc ggattcccgc
     1441 ccacttcgtc attcccgcga aagcgggaat ccggtgcgtt gggtttcagt catttccgat
     1501 aaactcctgc cgcattgtat ttctagattc ccgctttcgc gggaatgacg gcagataggt
     1561 ttccgtgccg tggtgtccgg attcccgccc gtacgggaat gacggcagtg cttccttggc
     1621 ttaattttat tttaatcaac tgcaaatgcc gtctgaaaac ctgtttgcag attttcagac
     1681 ggcattgttt tcgtcgccgg ttcaaacttc aatccgattg cgacacggta accggtatgc
     1741 cgcgcaagcg cgcaagcagc gcgtccgcct tctgtttctg cgcggcaaac gccgccttgt
     1801 ccgcctgcgt caattcgggt gtcggcaatg cgactgaaac aggattaacg ggctgcccgt
     1861 tgatgcgcgc ctcgtaatgc aggtgcggcc cggtcgaacg cccggtcgaa ccgacaaaac
     1921 cgatgacctc gccgccgcgc acattgcctt gtgcctgcga aaacgcgctc aagtgcgcgt
     1981 acagcgtttc cacgccgttg gcgtggcgta tcatcaccgc gttgccgtat ccgcccttcc
     2041 ggcctttaaa ggtaatcacg ccgtcggcgg aagccctgac cggcgttccc tgcggtgcgg
     2101 cataatcgat gcccgtgtgc agccgccatg tgtgcaggat ggggtgcata cggtagccga
     2161 acggcgaaga aatgcgcgta tagaccagcg gctcgatgtt gaagccgcct ttttcctgca
     2221 acaccctgcc gtcttcatca taataattgc cgccccctcc gccttccttg tccgaacggt
     2281 aatagaatgc ctgatgcctt gtgccgccct taacgacttc agccgccaaa atatcgcccg
     2341 ccgccacctg ctgcccgtgg aaatacaggc tgtcgtacat caggcgcacg gcatcgcctt
     2401 ccttcaaacc gtcaaggctg aagcggccgg cgaaaatccc gcttaaggat tcgcggattt
     2461 cgacgggcac ttccgcccgc gccagcgaac cgcgcgccga cgttttgacc acgaccgaac
     2521 gcagcgtcgg caaaaccttc atatccgcct cagaagccga ccgccgccat atgccgcctt
     2581 tcttttccaa agcgaccaga ttgcgctcgc cgtcttcgtc ggtaaaaaac tgtacttcgc
     2641 gcgcgccgcc gtcgccgccg accaaaacat gaaccgactg gtcggcacgc aaatgccgca
     2701 aatcggcttc gccgccatat ttttccgtta ttcgggcaat ttcgtcccgc gccatacccg
     2761 aacgcgccag cacgtccgcc agcgagtcgc ctggctgcac cgcctcctgc acccaatatg
     2821 ccgtctgaac accgctgccg ccccaagaca gcggcggcag tttttgttcc acgcgctgcg
     2881 gtctgacgcg ctccgtcctc tctgtcgaag caatgtatgc cgccgacacc aaaataatcg
     2941 aaacggcaag cgcgcgcagc gcgtatttcc gatgttttgc cgaaagtggg aagacagcca
     3001 tattgttcct ttttgacggc gtttgcgccg aatcctctat aatgttgcgc ctgacctgca
     3061 tttctgaaac gtcaaacgat gaaactcacc ctcgccattc cctcgctcaa tttggatgaa
     3121 gacgaaaccc gaatacctct ctgcctgccc gccttcaaca aaatcctgca atacggctcg
     3181 ctgcaccgcc aaagctgcac ggcttcggca ttttacgcgc gttatttgtg gtgcggcaga
     3241 ctggcggaac gcgcggcaca aagcctgaat atgccgtctg aagccgtcgc gctcgccacg
     3301 cccgtctggc aaaagatggg gatgcatcag gcaaatgtgc taacggcgga atatctgaac
     3361 gtcggtacgg atgaagcgga acggctctgc cgcgacctgt cggcgtttta cggcgacatt
     3421 ccatggcgtt tcgttcccgt cctgcccgaa ttatggctgg tttcccttcc ttgcgcgtac
     3481 cgctggggcg cgaagcccgt tttggacttg ggcggattgt tgggcgcgga cgatcagccg
     3541 gacggggaag acgcgttgga atggctgcgc gtccaaaccg aaatccaaat gtggctggcg
     3601 gcgcaccccg tcaaccataa ccgcaagaaa cgcgggctgc ccgagttgaa cgggctgtgg
     3661 ctttgggaca gtctgcacgg aagcagtcaa ggcggcacgc ttttcgccga tacggtttgg
     3721 agccgtttcc accccgaccg ccgcgcattg cccgacaact tccgcgccta tgaagaaacg
     3781 gcggcatacc tacccgacac gcaccatatc ctgtttatgg acgatttgcg cctgaccgcc
     3841 ctgacagggg acagggagcg gtatgcggca atattgcagc agtgggaaga acgctggttc
     3901 gcaccgcttt acgaagccgt ccgcatcggc aaaatcaaac ggctggacat cgccacggac
     3961 ggacaacacg gcggtacgct gaccttcaaa cccgccgaca ggtggaaatt ttggcgcggc
     4021 gcgaaaacct ttgacggaat ctggtgaacc cgcccgccgc aaccgcgaca atgccgtctg
     4081 aagcccgaat cgggtttcag acggcatttt acctcgcatc gccgttatgc cggaaaaacc
     4141 ggaatgcgtt tccgcacacg cggcggcacg caaagcaccg cccgaaagcc gccgatgccc
     4201 gccgtgatac cgccgccggc agggtttcct ccgaaaagga acagcaatca gaacgtttta
     4261 ttaaactcga caaacgcccg atttttctcg tattcgttga acacatcgtt actccgcgtt
     4321 tcgcggtgcg acaacgtcag gcgcggcgtg atgcctttga aatgcaatgc ccggtgccaa
     4381 aggctcaagg atgtgttcaa ttctttatcc ctgcgccttt cccctttaaa accgctgaaa
     4441 aagccgggtt tttcataatg ccgtttcgcc gcgccgaggc gcaacagcga agacaggccg
     4501 ctgccgcccc attcctgccc ccaggcaaag cgcaggccgt aacggttgaa attgtcgccc
     4561 cggtcggcgg ggttgcgctc gcggtaaaaa tccaaaccgc ccatccaata ttggcgcgca
     4621 ttccggtaaa acaccagcga attggaaatt tgcaaatggg tattgtcgga acgcgcccgg
     4681 cgcgtattct tcaaacgccc ccactccgcc gaagacaacg tttgccattt cggggtttgc
     4741 caacggttga aataaaggcg tgcgccgttg gtgtaagaat aagcgtcgtt gccgtaggtg
     4801 cggcgttcgt ggaacactgc cagcccggca tctttgcgcc ggtcggcaaa accgatgccg
     4861 ccggaaacgc ctgccgtcat atcgttgaat ttcttattcc ccggataaac cctgccggac
     4921 acgtcgccgc ccgccgtcgt gtaccagccg tttttcagcg accatttttt ctccgcgccg
     4981 agccggtaat tgaccgccgt gccgtccacc tgtttcggga aagtccattt gccgtactgc
     5041 tgccgtttcg gggcttggtt gatattgtgt tcgcgggtga cgctgaagcc gccatttacc
     5101 ttccacgcat cgcgttcgcg caatgccttg cggtacagct cgacctgctc catcagctgc
     5161 ggcggcaggt tttccgcctt caggcggtcg aactggtctg ccgccgcctc gttctgcctg
     5221 ttttcaaaca atgccgccgc caaacgcata cggacggcgg gcgcgtcggg ttgggcggca
     5281 atcaattccc ggtaatggga aatcgcctcc ttcaccctac cgtctgcctg cgccaaaatc
     5341 ccttgtgcat aaagtgccaa catcttatcc tgctgcgcct gttgtaggta aattggcaaa
     5401 ataacgcgga taccggcaat attgtttgaa accactgcgg aatacatcgc gcgggacagt
     5461 aattcgggat ttttcagcag ggtttcgccg tcaatctgca aaacttttcc tttttcccgc
     5521 acctgccccg gtaccttctc cctgtcgatc ggtttggcct ccgcttcatg aagcctgaac
     5581 tcgggacggc ttctcaaatc cggttcgcgc ggtgtttctt cggcatatgc cgcgcttgcc
     5641 aataaaggca gcagcagcat ccatctgttt cgtgcaggca taaatgtctt cccacaaaaa
     5701 taaaaaataa ccattatcaa ccggcggatt gtatcagttt tttatgccgc tgttccaaac
     5761 cctgccgccc gatgccgcga caatgccgtc tgaagccaga atcgggtttc aaacggcggc
     5821 aaaaaaccgg cggttttccg ccggtttcat catgatgccc cgcccttcgg ggcggcaggg
     5881 ttcggattgg tttgcggggc ggcgcgcggt gccgtgccgg tgtgccgcct gtacgtcaaa
     5941 acatttcccg gcgcatcaaa acagccattt gagcgacaat gcggcttctt tgtcgccgtc
     6001 cgtccttttg ccgtatccga tgcgtgcgga catatggcct ttccaaccgg cttcaatgcc
     6061 gaaccgccct tcgagcgccg tcctgcctgc cagcgtctgt ttttcgccgt ccatttccac
     6121 gccgaaagat tttgacctgt gcaaaacatt aaaagcggcg aaaggctgaa gattgacacc
     6181 gttacgcaaa gcaaaacggg tttttgcccg aataccggcg cggctttgcc actgaccgct
     6241 gccgagcagt ccgaccgccg tcccctcgct gtcggtaaag ccgccgttta cgcccaagta
     6301 ggtaaactga gcctgcggtt gcaggtaaaa ccgcacatta ttgccttttc cgacaacgcc
     6361 ttccgccaca agcgcgttgt agccgccttc gacagaagcc gtccaacctt tggttttgta
     6421 gcgttccaca cggttttcat cattgatgcg gtgtttgaaa cgttggtatt gcaaccagcc
     6481 gtccaaatac gcacccgttt gtttatcgcg caactgatgc cacgcagcat aaacaccccc
     6541 gccataacca tgcaaataac tgcctgccgc accgcctttg ccgttgactg atgcgtgctg
     6601 gccggccctg ccgcccatca cgccgattgc cagccggctg ccttcatttt gccgtacaaa
     6661 cacctcgccg ccgatttgca cgcctttgcg ccgcccgtcc gcagccgcgc cgccccgtat
     6721 attttgatgc gaccggccgc cgatgaagcg cagccacaat ttttgacggg acggatcggc
     6781 ggcatacacg tcgtctcccc tgtcggcggc acgcagccca aacaaagtat ttgccgcata
     6841 agcctgttgg gcatataaaa ccgctttcgc ctcggggata gccaaacgct gcacgcccaa
     6901 gcaaaatccg ccttcgcatt gcttgagcaa taattcgtac agtccctgcc ggtaattttg
     6961 tttaaacgca aacgaattag ggtctgcctt ttccgccgca atcagtttgc gcccgtcgag
     7021 cgttttcaga ttttccggcc ggcgttccaa aaccacgccg gattgtccgc tgactttgtc
     7081 ttcaaaggtc agggcgggtt cgtccgactc ccgcacgtcg tagccgaaca ggaaatccgc
     7141 gccgccctga tacgctttat ggacggtcag tttttcatct ttcgtgttca ggcggatccg
     7201 ggcattatcg gaagtttcca gcatatggat gtcggaatgg cggcgcggtt cccaaaggga
     7261 atctttcagc gtcatcttgg tcagttccaa cacctgtccg gcgacgtggg atttgttttc
     7321 gattttgacg tgtaattttt tattgaagat gttgttga
//
LOCUS       DAXHKX010000096         7350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHKX010000096 DAXHKX010000000
VERSION     DAXHKX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.096"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            37..180
                     /locus_tag="ACFMWZ_001430"
     CDS             37..180
                     /locus_tag="ACFMWZ_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935382.1"
                     /translation="MMLNQGFCEAFIVVVLMGIAAIMRKRPSFSKPPHRFRVCPSSRP
                     DLQ"
     gene            217..429
                     /locus_tag="ACFMWZ_001431"
     CDS             217..429
                     /locus_tag="ACFMWZ_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC5935383.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            577..2160
                     /locus_tag="ACFMWZ_001432"
     CDS             577..2160
                     /locus_tag="ACFMWZ_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC5935384.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDLLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2242..3339
                     /locus_tag="ACFMWZ_001433"
     CDS             2242..3339
                     /locus_tag="ACFMWZ_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC5935385.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3453..4568
                     /locus_tag="ACFMWZ_001434"
     CDS             3453..4568
                     /locus_tag="ACFMWZ_001434"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC5935386.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4693..5148
                     /locus_tag="ACFMWZ_001435"
     CDS             4693..5148
                     /locus_tag="ACFMWZ_001435"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC5935387.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5219..5695
                     /locus_tag="ACFMWZ_001436"
     CDS             5219..5695
                     /locus_tag="ACFMWZ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC5935388.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5683..6609
                     /locus_tag="ACFMWZ_001437"
     CDS             5683..6609
                     /locus_tag="ACFMWZ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC5935389.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMEQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVRNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNNLPKFTDGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6674..7231
                     /locus_tag="ACFMWZ_001438"
     CDS             6674..7231
                     /locus_tag="ACFMWZ_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC5935390.1"
                     /translation="MKPKFKTVLTALLLAASLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         1921 a         1818 c         1911 g         1700 t
ORIGIN      
        1 tgttaatcca ctataaatgc gccgattgtc aaggcgatga tgctcaacca gggtttttgc
       61 gaagcgttca ttgttgtcgt cctgatggga attgccgcca ttatgcgcaa gcgtccgtct
      121 ttttccaaac cgccgcaccg tttccgcgtt tgcccaagtt cacgccccga cttacaataa
      181 cgcccgtcag attgcagttc caacgaaagg aaaatgatgg aaacccttat cctcgacatc
      241 ggcggcatga gctgcggcgg ctgcgtcaaa agcgttaccc ggatactgga aagcgtcaaa
      301 ggcgtggcaa gcgtcgaagt cagccttgaa aacaaaagtg cgaccgtcgg ttacgatccc
      361 gcgcaaaccg atgcaggggc gttgattgaa gccgttgaag acggcggcta tgatgcggcg
      421 ttgaaataaa gcggcaaaaa tgccgtctga agccttcgcg cctccccgat gccgtctgaa
      481 gtacgctccg cgccttcaga cggcatttga tttagttgaa cgggtaggat gaattggtta
      541 aacttgcggg caatagtgcc ttcagacaaa caaaatatgc cgaacaaaaa gaaaaaagcc
      601 gcctttgcgc gtgctgcaac ggagaacggt gtgccggacg tgtccaacat caaaaattca
      661 tatgatgatt tgatgtatga atccaaagca ttttcccaaa ccgccatcaa cgccttggag
      721 gctcgcgccc gcatgatggg tttgcagccc gcgcccgccg ccaatgccaa ggtcttggag
      781 ctgggctgtt cgatgggcgg caacatcatc acgcaggctc tttattaccc ggatgcggaa
      841 tttgtcggta tcgacttgtc cggcaggcag gttgcgcagg gcaacgccat cattgaaaaa
      901 atgggcttga aaaatgtgcg tctggaagaa aaagatattt tgaccgtcga tgagtcattc
      961 gggaagtttg actatatcat cgtccacggc atttggtcgt gggtgcctga cgcagttaaa
     1021 gacaaaattt tttcgatttg ccggaacaac ctgaccgaac acggcattgc ctatatttca
     1081 tacaatgttt accccggctg gaaacggcag gagcagttgc gcgaaattat gtactttgcc
     1141 ggcagggatc tgcttgaaga acccttggaa gcgcggacgc ggaaaggctt ggacgcgctc
     1201 aaggcgctgg cggaaatttt ggaaaacgac aaaggcttgg gcggcggcgg caaacttccg
     1261 gcgattcaaa aaatattgaa tcacaatact tattatgtcg cacacgaata tatggaaata
     1321 ttcaacgacc cgatttacgt taacggcttc atcgaatggg caaaccgcca ccggctggcg
     1381 tatatcgggg ataccgattt gcacgtgtct tttgtttctt ggatggcgga gcatacgcgg
     1441 gagcggattt tggcattggc aggggatgat tatattgcca aagaatttta tagcgatatt
     1501 ttatccgacc gccaattccg ccgttcgctt ttatgccgtg aggaagtcgg ggacaatatc
     1561 aggcgtgatg agtcggttgc cgtcgaagtg atagaaagtt tgaatttccg cccggcaaga
     1621 ggggaaacga tcaactttga tgaaaacgat accctgcttt cgggcatacg cgatgtgatg
     1681 aaaaccggag agccgtttaa aacggaagat gttgcggaaa acctcgcccg ccgatttccc
     1741 ggtctggaat ttgaccgtat gaaaatcaat tcccagcttt tattgcaaac cattctcggg
     1801 cgtttttctg tttcatcaga caatgcgggc aaaccatttt ttgaagacca taaaacctat
     1861 gtgccggcgc gttttacaga ttatgccgcc gcctttgtgg aacacggcgc ggaagcgttt
     1921 gttcggcctg ccaaccgtta caacgaaagc accccctcat tcggatacgg gcatttgtac
     1981 attatgcggc aattatcgcg gccgacgagc aaacaggcat tgattgaaac ggttgccgaa
     2041 aacttgaaca tcgtcagcac cacgcccgac ggtttgacat tccatccgcc tgccgaagtg
     2101 tatgtggaag aaatattggc agacttggca gacaggcatt ttctcgtttc ggcggattga
     2161 cgggcaagtc ccggcggcaa tccgtccgat atattttcag acaggaaaga cacacactgc
     2221 gaaaagcggt acacgacata tatgaaccat acatttacct tgcccgatac gcgcccgtat
     2281 ccccaaaatc cgattaagaa ccacctgctg ctcaatgcct accagttggc gcacaattct
     2341 tcccaggctt cgcgcaaact ctcgtccggc caacttcaaa ccgaaatcag ggggatgctt
     2401 gagcaaaacc actatatcaa cctttccctc gcgctgacga tgtcgcccga tgccggaact
     2461 tatgccgcgc tgctttccag tgtgaacgcg gtactcgatt gcgagaaaga aggcgaagtg
     2521 cagtggttcg ccctgccggt cgtgctggtg tccggctgca agaaagaacg ggcaatcgag
     2581 atgaagctgc cgacggaggc attgtttgcc tgtttgcaaa actatccgca cctgcgcgcg
     2641 ttgacgcaag agacgcaatg gctgccttat cttgtacatt cctccgattt gagtgcggtc
     2701 gcgccggatg agtggtggcg tgccaaacaa aataccgaag cggcggcgca acacttacgc
     2761 cgtttcgccc cgcgcccttt gcttttgccc gaagggcagt ccgtccacgt cgtttacgcg
     2821 ctgggtttcg gcagcggcaa agttcagacg gcattgggtc agaaccttct tcaggcaggt
     2881 ctgcccttga tgcaggtatg gcaggaaaat cttgcatcgg aaggcgttac gctgtttgcc
     2941 aatccgcttt ccccggattc tccggtacgc gcgctttccg acggcagcca cacgcgccag
     3001 cgcatggcga tggatgtgtt tgcggcaaac gcgatacgcg ccgtccgtat gcagagtccg
     3061 cgcgtcggcg tggtcgcagc ggcaaaggcg ggcggacaga ttttattcgg gtttaatgcg
     3121 accgacggcg cgtttgaagt cgtgccgcag gtgttttcgt ggcagctttc ctttaccgac
     3181 aacatcgccg tcatccagca aaatttcctc gacctgatgg cggagtgccg cgtggaacac
     3241 gtttacctgt tgcacaatcc cttgggcgaa caggaaagca tcccgagcta tgcggaagca
     3301 ttgaaacggg aagggcacaa tccgtttttc agcgcatagt gatttgtagt agtgataggc
     3361 tttctcattt atataataaa tcaaaataaa gagcacaaca ctttttcatt ctgtgttgtg
     3421 ccttgagtga aacgaaagga tgaattataa atatgaagat tgtaattgca ccggattcgt
     3481 ttaaggaaag cttgacagct caacaggtag ctgaagcaat aaaaagaggc ttccaacaat
     3541 cgatagcaga tgtggaatgt ctcctctgtc ctgtcggtga tgggggagaa ggcactgtag
     3601 atgctatccg acattctctt gacctagaag aaaaatgtct tcaagtgaca ggaccttttg
     3661 gacaaaaaga agtcatgcgc tattttcaaa aagaacaact agccctattc gaagttgctg
     3721 atttggttgg tcttggaaaa atcccgctag agaaacgaaa tccattacaa atccaaactc
     3781 gtggtattgg agaattgatt cgccacctca ttagtcaaga aattaaagaa atctatattg
     3841 gcgttggcgg tacggccagt aatgatggag gtattgggat tgctgctggt ttaggttatc
     3901 aattttatga tgaggatgga aataccttgc ccgcttgcgg tcaagcctta ctaaacctag
     3961 cttctgtttc aacagaaaat cgctatgaaa ttcctgaaga tgttcacatt cgtattttag
     4021 cagatgttgt gagtccctta tgtggacacc aaggtgcgac ctatacgttt ggcaagcaaa
     4081 aagggttgga ttctactatg tttgaggccg tagatcaggc aatacaagat ttttatgaaa
     4141 aagtctcccc tgcaacatta aaacttaaag gagcaggagc tggtggaggc atcgctggtg
     4201 gtttgtgcgc ctttgctcag gcaagtatcg tatctggaat tgacacctgc ctggacttga
     4261 ttgactttga taagaaagtg tcagatgttg acttggttat cgttggtgaa ggaagactag
     4321 atcgtcaaag tttagcaggg aaagcgccta ttggtgtagc aaaaagaacc cctgtcggag
     4381 ttcctgttgt cgctatttgt ggcagtctcg ttgaagattt gccttccctg ccatttgaaa
     4441 atatccaagc tgccttttct attttggaga aaagtgaacc tttagaagat agtttgaaaa
     4501 atgccagtct ctatttggag catacggctt ctaatattgg acacttatta aatatgccta
     4561 agatttagcc aaaccatttc ttccagatgg atgttttggc tggttctgcc ttatgcggtt
     4621 acgattaacc gaccgatgcc gtctgaaagg gtttccgctt cagacggcat cgcgccggaa
     4681 cagggagggg atatgaaaaa accgaaaatc ctttttgtct gcctcggcaa catctgccgt
     4741 tcgccgatgg cggaatacat tttgcgccgc cgcgccgccg aagcgggcat tccccttgaa
     4801 gcggatagcg cggggacatc gggctggcac gacggcgaag atatgcaccg cgagacggca
     4861 aagatattga aaaaacacgg tatcgatgct tcaggcttta ccagccgcaa aatccgccaa
     4921 agcgatgcgg cggcgtttga ctgcatcatc gcgatggacg gcaagaattt gtccgaattg
     4981 gaaagaacct tcggcaggcg gtcggaaaaa atattcaagc tgaccgacct gatacccgaa
     5041 agcggttacg accatgtccc cgatccgtgg tatacgggcg attttgaaga aaccttcagg
     5101 cttgcggatg cgggctgtcg ggcattgttg gaaaagattt ccaaataaag caattgaata
     5161 caaatataaa accgcctcct gccgtatcgg ttgttcagac ggcataaaca gagaatttat
     5221 gaaaacaaat ttcaaacaga aaattatcga acaggcacgc agcgaaggct tgcaggtaac
     5281 cgctttgcgc gagcaggtat tagatattgt cttgcagcaa agcggcgtga ttaaagccta
     5341 caacgtcttg tcgcagatgc agcagcaaag cgagggcgtg cttgcaccgc ctaccgccta
     5401 ccgcgccctt gatttttggg cggagcaggg cgttttgcac aaagtggcgg cggtcaacgg
     5461 ctatattttg tgcagccacg cgcagcacga gtgcgacgac cattgccacg accacgaaga
     5521 agccgaagcg caccacagcg cgtttatttt ggtctgcacc gaatgtggca cggcggacga
     5581 gcaaaccctc agccacgagt gggcggcact gcgcgcaggc gttgccgaaa gcggctttgc
     5641 gctgaaagaa gaacacgttg ttttaaccgg gatttgtaaa aaatgtcaga aatgaaaaaa
     5701 accaaagtcc acctgatttc aggttttctg ggaacaggca aaaccaccgc gctcaaaagt
     5761 ctgatggaac aaaaagaccc caacgaaaaa tgggtcatca tcgtcaacga gttcggcgaa
     5821 atcggcattg acggcgcggt attgagcgac aacggcatcc ctgtggcaga aatcgccggc
     5881 ggctgtttgt gttgcaccgc cggcccgcaa atgggcgtaa ccgtgcagaa aatgctgcgc
     5941 gatgccaagc ccgaccgcct gatgattgag gcaagcggac tggcgcacgc cgccagtgtt
     6001 attgatgaac tgaaaaccaa accgctggac agccttctgg aaatcggcgc agtctttacc
     6061 gtcgtcgatc cgcgccagtt catcaacccc gattacgcgc aacaggcatt gtataaagac
     6121 caaatcggca tttgcgacgt attggccgcc agcaaaaccg atttatgcac ccccgaacag
     6181 cttgccgaat ttcacgacaa agcggcaaaa ctgttcccgc ccaaagccaa agtggtcgaa
     6241 gtccgaaacg cgcaactcga catccggtgg ctcgatattc ccgttatcga aaaatcacgc
     6301 taccgcctca aagccctgcc ggacaacacg atgggcttcc agtcgcaagg tttcacattc
     6361 cccgccggac gcgatttcga cggcgaaaaa ttgaccaact tcttcaacaa cctgcccaaa
     6421 ttcacagacg gcctcgtccg cgccaaaggc gtgttccaag tgctcggcac atgggtgtgg
     6481 ctcaactggg tggacgggca atggggcgcc aaccaagtgt cttggcggcg cgattcgcgt
     6541 tttgaattga ttgccaaatc gtttgatgcg gatttaatcg aacaaaagct caaagacgca
     6601 ttggaatagc gttcacgcgc cttcagacgg catatgaaat gaaaatgccg tctgaaggtc
     6661 tgaggaggac gggatgaaac ccaaattcaa aaccgtttta accgcgctgc ttttggcggc
     6721 ttccctgccg tctatggcgg caacccatgt tttgatggaa accgacatgg gcaatatccg
     6781 tttggttttg gacgagtcca aagcacccaa aaccgttgcc aatttcgtgc gctatgcccg
     6841 aaaaggcttt tacgacaata cgatttttca ccgcgttatc gacggtttcg ttatccaggg
     6901 cggcggattt accgaagact tggtacaaaa ggcaaccgat aaagccgttg ccaacgaatc
     6961 cggcaacggc ttgaaaaaca ccgccggtac catcgccatg gcgcggacgg ccgatccgga
     7021 ttccgccacc agccaattct ttatcaatct ggcggacaat gattcactca actacaaaaa
     7081 cggacaatac ggctataccg ttttcggcag ggtcgaaagc ggcatgaaca ccgtttccaa
     7141 aatcgcccgc gtcaaaaccg ccacgcgcgg cttttatcaa aacgtacccg tacagcccgt
     7201 caaaatccgt cgcgttgttg tcgggcagta acacgcagac agacgttcag acggcgtcgc
     7261 ccgtttccca aaaaacgccg tttaaagtaa aaaaatattt taaaacagac agttgatatt
     7321 gacaaattca aaccgaggat tttaaaatgc
//
LOCUS       DAXHKX010000097         7349 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHKX010000097 DAXHKX010000000
VERSION     DAXHKX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.097"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFMWZ_001439"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFMWZ_001439"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC5935391.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSTKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVASDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQTFPLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKVLLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGLSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2923..3717)
                     /locus_tag="ACFMWZ_001440"
     CDS             complement(2923..3717)
                     /locus_tag="ACFMWZ_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC5935392.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFAQAWK"
     gene            complement(3834..3968)
                     /locus_tag="ACFMWZ_001441"
     CDS             complement(3834..3968)
                     /locus_tag="ACFMWZ_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935393.1"
                     /translation="MPSERIFRSILRLVGVSSETVKQKFAGVELSYAGKAAGESLYCL
                     "
     gene            complement(4468..5337)
                     /locus_tag="ACFMWZ_001442"
     CDS             complement(4468..5337)
                     /locus_tag="ACFMWZ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC5935394.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAKRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKGSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREEDNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5388..5924)
                     /gene="ruvC"
                     /locus_tag="ACFMWZ_001443"
     CDS             complement(5388..5924)
                     /gene="ruvC"
                     /locus_tag="ACFMWZ_001443"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC5935395.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5927..6166)
                     /locus_tag="ACFMWZ_001444"
     CDS             complement(5927..6166)
                     /locus_tag="ACFMWZ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC5935396.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6196..7206)
                     /gene="dusB"
                     /locus_tag="ACFMWZ_001445"
     CDS             complement(6196..7206)
                     /gene="dusB"
                     /locus_tag="ACFMWZ_001445"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC5935397.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCREFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHEDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIGEMPDGEQARREINRLDSAAAQYDMLAGYLERLAGKTDRWACGYRTN
                     AF"
BASE COUNT         1652 a         1938 c         1941 g         1818 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggc tcgagcttgt tgcagaggtt gaacgcttgc
      241 accaaacggg cggcgacctg cgggttaaag cggtcgattt cgatgacttt gtcggcgatg
      301 aagcggtagc cgctgccatc ttctgcgtgg aaatgcggga cgttgcggct gaagctgccg
      361 atgagcgaac gggctttgtt ggggttttcg aggctgaatt tcggatgctg caaggcggtt
      421 tggacctgtt gcagggtgtc gctgcggcgg cttaagccga cgagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgag ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttgac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcgccgt atttttcggc aacggtttcg atgtgcgcgg ggtcggcgcg caggacgaag
      661 gcgcggcaga cgttgcgcag cgtgcgccag ccggcagctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag gaagcggacg
      781 gcaagcgtat ccaacaaggc ttcgcgcgcc tgatggtagc gcagcgggtc gatgttttct
      841 gcgccgtccc acagttcggc ttcagacggc acacccaaaa gcaggacttt gaaggcgttg
      901 tccaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc gtgtttcggc
      961 aactcgatgc cgtctgaaag cgcggcaagg ttggcggcga cggcgcggcg gtagagcgtt
     1021 tgggcggctt cccagcgcgt gaaagcgtcg ctgtcgtggg caagcaggag cagcaggtcg
     1081 tcgtcgctgt acggatagtt cagatgcacc ggcgcactga acccgcgcag cagcgaggga
     1141 acgacggctt cggttacacc ttcgagcggg aaggtctgtt cggcttcggt cagcagcaat
     1201 acggcttcgg tcgcgcgttt gccctgataa tcgaatgccg ccgcttcacc gttgcggttc
     1261 agcagcccga ttttgacggg aatcatcatc ggctgtttgt ccgccatatc gggcgtgggc
     1321 ggcacggttt gtttaatggt taactcgaaa atattgtttt tcagacggcc ttccgcttcc
     1381 aaaacgggcg tgcccgcctg actgtaccac aaggcgaact ggtcgagatt gatgccgttc
     1441 gcgtccgcca tcgccgcgcg gaaatcgtcg caggtcactg cctggccgtc gtggcgttgg
     1501 aaatagagct tcatgccttt ttggaagccc tcttcgccga gcaaggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatag
     1621 ctggcggggc gcaccggatg ggcggtcggg cctgcgtctt cggggaactg gtgctggcgc
     1681 agcaggcgga tgttctcgat gcggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcaa cccttctttc agcgaaagct ggaaccaatc gcggcaggta
     1801 acgcggttgc ctgtccagtt gtggaaatac tcgtgtccga ccacggattc gatgccttca
     1861 aaatcggtat cggtggcggt acggctgtcg gcaaggacga acttggtatt aaaaatgttc
     1921 agccccttgt tttccatcgc acccatattg aaatcgccca cggcgacgac catgaaaata
     1981 tccaagtcgt attccaaacc gaagcgcgtt tcgtcccact tcatcgcgtt tttcaaggat
     2041 tccacggcaa agccgacctt aggcttgtcc gcctcggcag tgtaaaactc gattttgacg
     2101 tttctgccgc ccatcgtggt gaaacagtct tccgtgaccg ccaaatcgct cgcgaccaaa
     2161 gcaaacagat agctcggttt ggcaaacggg tcttcccatt tcacccaatg gcggccgtct
     2221 gaacactcgc cgccgtcgat tttgttgccg ttggagagca aaacgggata gcgttttttg
     2281 tccgcaacga tggtggtcgt gaatttggac atcacatccg gacggtcgat gtagaacgtg
     2341 attttgcgga agccctccgg ctcgcattgg gtaaacagat tgccaccgga agcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atctcggttt ccacttcgac ggtgaagcgt
     2461 tcggacggca cgtctgcaat cgtcagagtc tcgccttcca acacataatc cgccgccacg
     2521 ccgttgattt tgacggacaa gagtttcgtc gaaccgtcca acaccagcgg ctcccccacc
     2581 ctctgcggct cgaccgtcaa acgggatttc acgacagttt gcggttcggc aatatcaaaa
     2641 tgcaggtcgg tttcaaggat gcggtaggca ggcgtttggt aatctttcag ataacggacg
     2701 gttttgctca tttttttctt tcaatattgt ttggtttgac tggaaaaggc ttcagacagc
     2761 acggatacag cttttaaagc ggattaacaa aaatcaggac aaggcgacga agccacagac
     2821 agtacagata gtacgggctg taccggtttg aatttaatcc accatatgcc gtctgaaacc
     2881 gaaacggcgg cacaagcgta ccgccttcgg acagcaggtt ttctatttcc acgcttgggc
     2941 aaattctgac caatgcggtt tatcttctgt ttgggcggaa agatagtttc tgatccgctt
     3001 gatttccatt tcgtattcgt ccgcgtcgaa tctgccgctg accagacaaa aacgcagata
     3061 catcaaatac gtattcacgg catcggtttc gcaataattg cggatttctt tcagcctgcc
     3121 cgcgtggaac gcatcccaca ccttgctgcc gtccataccc agcttgcccg gaaaaccgca
     3181 cagtttcgcc atatcgtcca gcggcacgtt tgccctcggc tggtaaagcg cgagcaaatc
     3241 catcaaatcg cagtggcgtt ggtgataacg gctgatgtag ttgttccact tgaaatcgcg
     3301 gctgtcgccg aaatcgccgt cgcccatatc ccaatagcgc gcggcgttaa tgccgtatat
     3361 cagggagcgg taatgcagaa ccggcaggtc gaaaccgccg ccgttccaac tgaccagttg
     3421 cggcgtatgt ttttcaatca attcgaaaaa tttggcaatg accacttcct cgccgtcatc
     3481 catctcgccg attgtaccga catggacttt atcctgcccc caacgcatac agcacgaaat
     3541 cgccacaacc tgatgaagat gatgctgcat aaaatcgccg cccgtttggg cgcggcgttt
     3601 ctgctgggca aacagcacca cttcatcgtc gggcagcgag gacggcagct cgtacaatgt
     3661 tcggataccc tgcacatcgg gtacggtttc aatatcgaaa gccaaaatcg tattcatggc
     3721 agtaccttgc attcaaaaac agacttgcgc ctattgtgtc attaaaaggc ggataaaaaa
     3781 agaggcaacc ccccacagga ttgccccaat acctcaaatc agagatttac gcttcacaaa
     3841 caatacaggc tttcgcctgc ggctttgccc gcgtagctca actctacgcc ggcaaacttt
     3901 tgtttcaccg tttccgatga aaccccgacc aatcgcaaga ttgaccggaa aatcctttca
     3961 gacggcattt cctgcctgtc gtgtaattcc atgtagcgaa atgtacgcca ttttctacgc
     4021 tttgccaaac attttttaca atataaatgt caaaacatta attttataaa attgctgaaa
     4081 atattaaata tatgaatttt tattttaaat attttaataa atataaattt aattttgatt
     4141 tatatttaaa tttaagcata aaatgttaaa tattaaaggc aatatgaaag gcatatatta
     4201 aatatttatt tataacgcta tgtttttaaa gaaaattaat tttaatatat taactagatt
     4261 gtctgcagat attcatgggt ttgcggtatt tcttccaaaa cctgcttcga attttccgag
     4321 caagtcttaa aaatattgtt ttttgagata cttaaatagc agcgattatc aaatgaaatc
     4381 tgttcatata atctgccatt tcgcatttaa aaaaacaaac aggagtttcg actcgaaacg
     4441 cctgatatgt tttgtaattt tacgtagtca gtaaaaatcg gggctgcctt ccggacgggt
     4501 tttgaaacgc ttgtgcagcc aaaaatactg ctcgggatgt tcgcgcacgc gttcctcgat
     4561 aaaacggttc atacgctgcg cgtcggcttg cgcatcttca ctcggaaagg attcccaagc
     4621 agggtagaaa tgcaatgtaa ccgtattgtc ctcctccctg acgggaatgg cgggtatcac
     4681 ttttgcattt gcaagcgcgg caatgcggct caatccggta atcgttgccg tctgaatacc
     4741 gaaaaaatcc acaaaaaccg aatcgttgcg tccgaaatcc tgatcgggca gatacagaaa
     4801 cggcgcgctg cctttgcgga actgtttgac gagggcgcgc agcccttcgg tgcgcccgat
     4861 aaggaaaacg ttgtgataac ggttgcggcc tttcaaaatc tgttcgtcca atatcttgtt
     4921 tttttggtgg gaatacatac taattagcgg aacatcctga ttaagcgcgt acaccgccat
     4981 ctcgaacgcg gtaaagtgcg gatacaggat gatgactttt tcccccgccg ccagcgcgtc
     5041 gtccaaataa tgcttattgc ggtaacgcac cagcgatttc aggcgtttgg caggcgcata
     5101 ccaatataag ccgtattcaa gcatcagttt cgccatatgt ttgaaatgct gcttcaatac
     5161 ggtttcgcgc ttttttccgt cccactcggg aaagcatttt gccaaattga tttcgccgat
     5221 acggcggcgg ggtttgacca aaaggtaggc gagcaaaccc gtcaggtcgg caagtttgtg
     5281 cagcagcgca aacggcagaa actgcaaaac atacagtaca aaaaatataa atttcatctc
     5341 gatacacatt ttcttttcag acggcaaaat acaaatgccg tctgaaacta ttgaaacctg
     5401 ccgcgcttga cctgcatccc cgaaggattg agtttggcgg caagcccgtg gttgcgtaag
     5461 gcgtgggtca gcgcgacggc aagaccgtcc gccgcatccg cctgcggcgt tcccgaaagc
     5521 cccagcatct gcaccaccat atgctgcacc tgttctttcg ccgccttgcc tttgccgact
     5581 accgcctgtt tgacctgcaa ggccgtgtat tccgaaacgg gcagcttatg gctgaccaat
     5641 gccgccaatg ccgcgcccct agcctgaccc agcatcagcg tcgatgccgg attgacgttg
     5701 acgaacacct gttccaccgc cgcctgctgc ggcttgtaaa cggcaacgac ttcgccgatg
     5761 tgccgcacaa tcacggcaat cctgtctgcc agaggcgcat cgggaggcgt tttgatgcag
     5821 ccggaggcga cgtaaaaatg atcgcgcccc ctgacatcga tgatgccgaa acccgttacg
     5881 cggctgcccg ggtcgatacc taagatacgg acggttgccg acatattcac agcaaaccgt
     5941 gttgaatcag ttttttacgc aaagtattgc ggttcagtcc caacatgacg gaggctttgg
     6001 actggttgcc gccgcattgc tccatcacgc acaccagcag cggcttttcc acctgatgca
     6061 ataccatatc gtacacgccg caaggttcgg taccgttcag gtctttgaaa tattgttcca
     6121 aattttgtct gatacattgg gaaatatcgg gaagggtatg gggcatgatt gcactttcaa
     6181 aggataatca agtgttcaga aggcatttgt gcggtagccg cacgcccaac ggtcggtttt
     6241 tccggcaagt ctttcaagat aacctgcaag catgtcgtat tgcgccgccg cactgtccaa
     6301 gcggttgatt tcacgccgcg cctgttcgcc gtcgggcatt tcgccgatgt accaacctat
     6361 gtgtttgcgt gcgatgcgta cgcctgcact atcgccgtaa aacgcgtgta tggcgcggat
     6421 gtggttcaaa atagcggcgg cgcattctgc caaactcaag gcaggcggta aaacaccgtg
     6481 ttcggcgtaa tgtttcaaat cgcggaagaa ccacggtctg ccttgcgcgc cgcgccctat
     6541 catgatgccg tcggcggcgg tttgtttgag gacggcggcg gctttttgcg gcgaagtgat
     6601 gtcgccgttg acccagaccg ggatgttcag acggcatttg gtttcggcga tgagttcgta
     6661 acgcgcctcg cctttgtaca tttgcgtgcg cgtgcgtccg tggacggcaa gggcggcgat
     6721 gccgcaatct tcggcgattt tggcgatgac gggcaggttt tgatggtctt cgtgccagcc
     6781 caaacgggtt ttgagggtaa cgggtacgcc tgccgcacgg acgacggctt ccaaaatggc
     6841 ggcaaccagc ggctcgttct gcatcagcgc gctgccggct tggacgttgc acactttttt
     6901 ggcgggacag cccatattga tgtcgataag ctgcgccccg aggctgacgt tgtaacgcgc
     6961 cgcatccgcc atctgctgcg gatcgctgcc ggcaatctgc acggcaacga tgccgccttc
     7021 atcgacaaaa tcgctgcggt gcaaggtttt tctagtattt ctgagcgtcg ggtcgctggt
     7081 cagcatttcg cacactgccc aacccgcgcc aaactcgcgg caaaggcggc ggaagggttt
     7141 gtcggtaatg cccgccatcg gcgcaagtgc gatggggttg tcgataaaat agccgccgat
     7201 gtgcataatg ggtccgcgtt tcaaaaaagt gcgacattgt acatttttta agcaggattt
     7261 ccaatctccg gacgcgcccg cgattggggc gggcaccgtt ttatggcata atccgcacac
     7321 agatttcctg ccccgccact cacaggcgg
//
LOCUS       DAXHKX010000098         7330 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHKX010000098 DAXHKX010000000
VERSION     DAXHKX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.098"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            95..814
                     /gene="pyrH"
                     /locus_tag="ACFMWZ_001446"
     CDS             95..814
                     /gene="pyrH"
                     /locus_tag="ACFMWZ_001446"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC5935398.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            998..1960
                     /gene="mafA"
                     /locus_tag="ACFMWZ_001447"
     CDS             998..1960
                     /gene="mafA"
                     /locus_tag="ACFMWZ_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC5935399.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            2000..3445
                     /gene="mafB"
                     /locus_tag="ACFMWZ_001448"
     CDS             2000..3445
                     /gene="mafB"
                     /locus_tag="ACFMWZ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC5935400.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            3411..3794
                     /locus_tag="ACFMWZ_001449"
     CDS             3411..3794
                     /locus_tag="ACFMWZ_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935401.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            4011..4727
                     /locus_tag="ACFMWZ_001450"
                     /pseudo
     CDS             4011..4727
                     /locus_tag="ACFMWZ_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            4732..5091
                     /locus_tag="ACFMWZ_001451"
     CDS             4732..5091
                     /locus_tag="ACFMWZ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC5935402.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            5167..5379
                     /locus_tag="ACFMWZ_001452"
     CDS             5167..5379
                     /locus_tag="ACFMWZ_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935403.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            5476..6255
                     /locus_tag="ACFMWZ_001453"
                     /pseudo
     CDS             5476..6255
                     /locus_tag="ACFMWZ_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            6252..6569
                     /locus_tag="ACFMWZ_001454"
     CDS             6252..6569
                     /locus_tag="ACFMWZ_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935404.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            6639..6932
                     /locus_tag="ACFMWZ_001455"
                     /pseudo
     CDS             6639..6932
                     /locus_tag="ACFMWZ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            6927..>7330
                     /locus_tag="ACFMWZ_001456"
     CDS             6927..>7330
                     /locus_tag="ACFMWZ_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935405.1"
                     /translation="MQTKALGTVDEIGDTVQQVGKQASGQKTSGGNPAIDSDPYSPSS
                     VAARIEAGKARSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDGRAVNARNDSSGGEPTLEIT"
BASE COUNT         2380 a         1470 c         1603 g         1877 t
ORIGIN      
        1 cagaacgaca caccatatca ttgtaattcc gaccgtttga aaattcagac ggcccaaact
       61 ccgaccgacg acattcaaga aagcaaggta tcccatgaca cagcaaatca aatacaaacg
      121 cgtattactg aaactctccg gcgaatccct gatgggttcc gatccgttcg gcatcaatca
      181 cgataccatc gttcaaactg tcggcgaaat tgccgaagtc gttaaaatgg gcgtgcaagt
      241 cggtattgtt gtcggcggcg gcaatatttt ccggggcgta tccgcccaag caggcagcat
      301 ggatcgcgcc accgccgact acatgggcat gatggcgaca gtgatgaacg cgttggcact
      361 caaagacgca tttgaaactt taggcatcaa agcgcgcgta caatccgcac tgtctatgca
      421 gcaaatcgct gaaacttacg cccgtcctaa agccatccaa tatttggaag aaggcaaagt
      481 cgtgattttt gccgccggta ccggtaaccc gttcttcacg accgacactg ccgccgcatt
      541 gcgcggtgcg gaaatgaact gcgacgtgat gctcaaagcc accaacgttg acggcgtgta
      601 caccgcagac cccaaaaaag actcatccgc cacgcgctac gaaaccatta cttttgacga
      661 agccttgttg aaaaacctca aagtgatgga tgccaccgct ttcgccctct gccgcgaacg
      721 caagctcaat attgtagtat tcggcattgc taaagaaggc tcgctcaaac gtgtagtaac
      781 cggtgaaaac gaaggtacgc tggttcactg ctgattgacc atagtgtcgg cagatatagt
      841 cgcatatggg cttcagacag ccatttatta tatggagatt atagtggatt aaatttaaac
      901 cagtacggcg ttgcctcgcc ttgccgtact ggtttaaatt taatccacta tatttacaat
      961 tttgatacaa tttgtttttc atcaaaggag aaaatctatg caagcacggc tgctgatacc
     1021 tattcttttt tcagttttta ttttatccgc ctgcgggaca ctgacaggta ttccatcgca
     1081 tggcggaggt aaacgctttg cggtcgaaca agaacttgtg gccgcttctg ccagagctgc
     1141 cgttaaagac atggatttac aggcattaca cggacgaaaa gttgcattgt acattgcaac
     1201 tatgggcgac caaggttcag gcagtttgac agggggtcgc tactccattg atgcactgat
     1261 tcgtggcgaa tacataaaca gccctgccgt ccgtaccgat tacacctatc cacgttacga
     1321 aaccaccgct gaaacaacat caggcggttt gacaggttta accacttctt tatctacact
     1381 taatgcccct gcacgctcgc gcacccaatc agacggtagc ggaagtagga gcagtctggg
     1441 cttaaatatt ggcgggatgg gggattatcg aaatgaaacc ttgacgacta acccgcgcga
     1501 cactgccttt ctttcccact tggtacagac cgtatttttc ctgcgcggca tagacgttgt
     1561 ttctcctgcc aatgccgata cagatgtgtt tattaacatc gacgtattcg gaacgatacg
     1621 caacagaacc gaaatgcacc tatacaatgc cgaaacactg aaagcccaaa caaaactgga
     1681 atatttcgca gtagacagaa ccaataaaaa attgctcatc aaacccaaaa ccaatgcgtt
     1741 tgaagctgcc tataaagaaa attacgcatt gtggatgggg ccgtataaag taagcaaagg
     1801 aattaaaccg acggaaggat taatggtcga tttctccgat atccgaccat acggcaatca
     1861 tacgggtaac tccgccccat ccgtagagac tgataacagt catgaggggt atggatacag
     1921 cgatgaagta gtgcgacaac atagacaagg gcaaccttga ttcacacgac cataaccgct
     1981 tgctaccaag gaaaacaaaa tgaaattgcc tattcaaaaa ttcatgatgc tgtttgcagc
     2041 ggcaatatcg ttgctgcaaa tccccattag tcatgcgaac ggtttggatg cccgtttacg
     2101 cgatgatatg caggcaaaac actacgaacc gggtggtaaa taccatctgt ttggtaatgc
     2161 tcgcggcagt gttaaaaatc gggtttacgc cgtccagaca tttgatgcaa ctgcggtcag
     2221 tcctgtactg cctattacac acgaacggac agggtttgaa ggcattatcg gttatgaaac
     2281 ccatttttca ggacatggac atgaagtaca cagtccgttc gatcatcatg attcaaaaag
     2341 cacttctgat ttcagcggcg gtgtagacgg tggttttact gtttaccaac ttcatcggac
     2401 agggtcggaa attcatccgg aggatggata tgacgggccg caaggcagcg attatccgcc
     2461 ccccggagga gcaagggata tatacagcta ttatgtcaaa ggaacttcaa caaaaacaaa
     2521 gactaatatt gtccctcaag ccccattttc agaccgttgg ctaaaagaaa atgccggtgc
     2581 cgcctctggt tttttcagcc gtgcggatga agcaggaaaa ctgatatggg aaagcgaccc
     2641 caataaaaat tggtgggcta accgtatgga tgatattcgc ggcatcgtcc aaggtgcggt
     2701 taatcctttt ttaatgggtt ttcagggatt gggagttggg gcaattacag acagtgcggt
     2761 aaacccggta acctatgcgg cagcacggaa aactttacag ggtattcaca atttaggaaa
     2821 tttaagtccg gaagcacaac ttgcggccgc aaccgcatta caagacagtg cttttgcggt
     2881 aaaagacagt atcaactctg ccagacaatg ggctgatgcc catccgaaca ttacagctac
     2941 tgcccaaact gccctttccg tagcagaggc tgcaggtacg gtttggagag gtaaaaaagt
     3001 agaacttaac ccgaccaaat gggattgggt taaaaataac ggctataaaa aacctgctgt
     3061 tcgccttatg cagactgtag acggggagat ggctggggga aataagccac cgaaaccaag
     3121 tacgcagcaa cactctacac actctgataa caatataggc ttacctgccc catatgttaa
     3181 acctgataca tctatttctc cgacaggaac aatttatgta gacagcaaag gaaatgcaat
     3241 accaacacca ataggcggtt atataacagg ttcgcctgat gctcgtttta tccaagtccg
     3301 agatagtaat ggaaacttga ctggagccag aatagatgat gctcatcatg caaataccca
     3361 tcaagaccca cgcgcactta caggacacgc acatgtacca ggagttacaa atgatgacgg
     3421 aaccccatgg ttacccatca aataatttac ctgttaaaat cataaatgat attatttatg
     3481 caacccagtt agttgaagat ttggttttag gaaaaataaa aattgttgat tttttaaaat
     3541 catataacaa tttttattat tggcttggtt ttgatgagtt acctcaatct gagaaaataa
     3601 aatttctaaa ctatctcaat atattaagta ttcataagga aatacaagat aaaactgtga
     3661 atagggttta taccgattgt tttgatattg ataaattaca ttcgcttggt agaattacaa
     3721 ctaatgaatg tattaatggt ataaaaaaaa tctatcaaaa aaacaaatta gaatttgaca
     3781 atatactcaa ataactagcc tatgttttgt aaaataaaaa tattcttgct gagtgtagag
     3841 ccagagataa aaagatttga tagtagtttg cagttgtaga attatataaa caagaaaata
     3901 gtcagcagcc gtaggtcaga ttctcgaatc cgacattttc caacagcggc atttcggaaa
     3961 cgatagatgc gtcaaatatt tttgtcggat acaaatatcc gacctacatc tctgcgcagc
     4021 agactctaca aggtattaat gatttaggaa atttaagtcc ggaagcacaa cttgccgccg
     4081 tgagcctatt acaggacagt gcttttgcgg taaaagacag tatcaactct gccagacaat
     4141 gggctgatgc ccatccgaat ataactgcaa cagcccaaac tgcccttgcc atagcagagg
     4201 ccgcaggtac ggtttggggc ggtaaaaaag tagaacttaa cccgactaaa tgggattggg
     4261 ttaaaaatac cgattataaa acacctgctg cccgacctat gcagacttta gatggggaaa
     4321 tggccggtgg gaataaaccg cctaaatcta taacgtctgg aggaaaagcc aatgctgcaa
     4381 cttatcctca attagttaat caattaactg ggcaaaactt aaaaaacatt gcggctcaag
     4441 atccaagatt gagtctagct cttcataaga gtgaaaaaaa ttttccaata ggaactgcaa
     4501 cttatgaaga ggcagatagg ctaggtaaaa tttgggttgg tgagggtgca agacaaacta
     4561 gtggaggcgg atggttaagt atagatggca ctcgacaata tcggccacca acagaaaaaa
     4621 attcacaatt tgcaactaca ggtattcaag caaattttga aacttatact attgattcaa
     4681 atggaaaaag gaataaaatt aaaaatggac atttaaatat taggtaatta tatgagatta
     4741 aaattaaaaa attatgattg ttctgacttt gatttggaag atttcccaca agacaagtta
     4801 gaaaattttt gtatactttt gacattatca ataggttttg atgaaagtaa tggtgctgat
     4861 tatttttatg tttatatata tagcactgaa tggttacttt caaatataca tagaccgatg
     4921 tcattaaaaa actcaattgt tacaaaccgt tttaatattg aacacatatt aaaattgata
     4981 aatgatatat tggaaatatg taattcaacg tctgaagata agtctatatc aaatttggca
     5041 aaatattttg actgggaatt tgacgattat aatctaaata tccaagacta aagatggtgg
     5101 acagcattta gaccaaaaac aggcaaacgg tattttgatg acgaattaaa ataaaaggaa
     5161 ttattcatga aattaattaa ttttgaagag agttcagtta aaattagtct cgataaagat
     5221 gagttgtata ttattcaagc aattgtagga gaaatttatt caggcgtatg cgtggattgt
     5281 agagattttg agataattca tggtgttgaa aaaataaagt tctttctttg gataaagaac
     5341 ttaaaaaaat atatgatact tgggataaat gttagctagg taaggtgggc acttgttgcc
     5401 caccttttgc cgtatattaa aaatcctttt caggctgcct gaaactgggt gggcatcaat
     5461 gccaatccta actgtgctga tgaagcagga aaactgatat gggaaaacga ccccaataaa
     5521 aattggtggg ctaaccgtat ggatgatatt cgcggcatcg tccaaggtgc ggttaatcct
     5581 tttttaatgg gttttcaagg agtagggatt ggggcaatta cagacagtgc ggtaagccct
     5641 gtcacagata cagccgctca gcagactcta caaggtatta atgatttagg aaaattaagt
     5701 ccggaagcac aacttgctgc cgcgagccta ttacaggaca gtgcctttgc ggtaaaagac
     5761 ggcattaatt ccgccagaca atgggctgat gcccatccga acattacagc tactgcccaa
     5821 actgcccttg ccgtagcaga ggccgcaggt acggtttgga gaggtaaaaa agtagaactt
     5881 aacccgacta aatgggattg ggttaaaaat aacggctata aaacacctgc tgcccgccct
     5941 atgcagacgt tggacggtga gatggcagga ggaaacaagc cagttgttaa atctatcaga
     6001 ccaactacgc gagatgaatt acgtcaagca ttgcaagaac aaggttttag acgtactggt
     6061 tcagatgagg ctcaatatga aacatggaaa ggtcctgatg gcgtgaaaat agatattcgt
     6121 ccaaatggag aggttataag aacccaaaga gtgccgcgaa ccgatggtgt acagggaaaa
     6181 tatccgcaac gacaaaatta tgaaggcaat ccattgccaa ataatcgtca tcattctgga
     6241 tattttatca aatgaaaaaa aatatttttc acaatgtaag cctttatgaa ataatctttt
     6301 ccgataatgg aaataccctt acattatctt ttacagatac aattgaaggt aattatttcg
     6361 gatatatcaa atgcagtaat attttgaatt ttaaattaga tacaaataat ttcgtagatt
     6421 atgaggataa ggaagatagc ttgtttccct tgtttatacc cgaaatagag ctatataaat
     6481 accaatttta tagtgaaatt attattgatg tagggattat tataaaaata tctgctgaaa
     6541 caattaattt tgagccactg ggaaaatagt aactgctttc ccagcagccg tagcaactgt
     6601 atttttaccc gacggggtaa aaatacagtt gctacatctc tgcgcagcag actctacaag
     6661 gtattaataa tttaggaaaa ttaagcccgg aagcacaact tgctgccgcg agcatattac
     6721 aggacagtgc ttttgcggta aaagacggca tcaattccgc cagacaatgg gctgatgccc
     6781 atccgaatat aacagcaaca gcccaaactg cccttgccgt agcagaggcc gcaggtacgg
     6841 tttggagagg taaaaaagta gaacttaacc cgaccaaatg ggattgggtt aaaaataccg
     6901 gctataaaaa acctgctgtt cgccatatgc agactaaggc gttaggtacg gtagatgaaa
     6961 ttggcgatac agtacagcag gttgggaaac aggctagcgg acaaaaaacc agcggtggta
     7021 atcctgcgat tgatagcgac ccctatagcc cgagtagtgt ggcagctcgc atagaagccg
     7081 gtaaggcgcg cagtgattta caaatcaaag acattttgag caatactact caaaggagta
     7141 aaacaaaagg tcccgctgtt cagtatgata aagtggggga ttacaatgac gcactaaatg
     7201 attttaatag tctgaatgtt cgaaatgtac aaacacgtcc taatggaacg ataacgggca
     7261 atttacctga tgggcgtgcg gttaatgctc gtaatgatag tagtggtgga gaaccaacac
     7321 ttgaaataac
//
LOCUS       DAXHKX010000099         7270 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHKX010000099 DAXHKX010000000
VERSION     DAXHKX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.099"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(135..1997)
                     /gene="hscA"
                     /locus_tag="ACFMWZ_001457"
     CDS             complement(135..1997)
                     /gene="hscA"
                     /locus_tag="ACFMWZ_001457"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC5935406.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGGNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMPIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2168..3040
                     /gene="hpnD"
                     /locus_tag="ACFMWZ_001458"
     CDS             2168..3040
                     /gene="hpnD"
                     /locus_tag="ACFMWZ_001458"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC5935407.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTENFAKLMAFQIERARETYCE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3119..4432
                     /gene="hpnE"
                     /locus_tag="ACFMWZ_001459"
     CDS             3119..4432
                     /gene="hpnE"
                     /locus_tag="ACFMWZ_001459"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC5935408.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            4516..5235
                     /locus_tag="ACFMWZ_001460"
     CDS             4516..5235
                     /locus_tag="ACFMWZ_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC5935409.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            5400..6071
                     /locus_tag="ACFMWZ_001461"
     CDS             5400..6071
                     /locus_tag="ACFMWZ_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC5935410.1"
                     /translation="MKTVSTAVALAAASVLLSACASESSRSLEVATVASYSTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNRKIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            6081..6449
                     /locus_tag="ACFMWZ_001462"
     CDS             6081..6449
                     /locus_tag="ACFMWZ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC5935411.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            6446..7093
                     /locus_tag="ACFMWZ_001463"
     CDS             6446..7093
                     /locus_tag="ACFMWZ_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC5935412.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
BASE COUNT         1598 a         2122 c         2024 g         1526 t
ORIGIN      
        1 cactatacac gcccttgatg acggttttca tcgaatagac aatgcctgac ttttccatat
       61 cgatgttttt caagtgtttt cgagccttca gacggcatca ggttatatct atgccgtctg
      121 aaaccgttta agtatcaaat attatcgaca ctctggcctg tcaatgcgcg ttggatattg
      181 cggttcatgc gtttggcggc gaagttgtcg gtgctgcggc tgagtttggc gacggcggag
      241 cggatgtctt cggcttttcc gtctttcaga cggccttgca aatcggcgat gccttgccgg
      301 atctgttgca attctttggc atccagtaaa tcgctgtcca actcgagggc ggcgttgacg
      361 gcatcggtca ggctttcggc ttcgactacg gcttcggcgc gggcgcgtgc cgccatatct
      421 tcggcggcgt tgtccatact gtctttaagc attcgggtaa tcgcgccgtc gtccaagccg
      481 taggaaggtt tgacttcgat ttgcgcctgt acgccggtgc tttgttcttg ggcggaaacg
      541 gacagcagcc cgtccgcatc gatttggaag gttacgcgga tacgcgccgc acccgccgcc
      601 atcggtggaa tgccgcgcag ggtgaatttg gcaaggctgc ggcagtcaga aacgagctcg
      661 cgctcgcctt gcacgacgtg tatcgtcatc gcggtttgac cgtctttgaa ggtggtaaag
      721 tcctgcgcgc gcgcggtggg gatggtggaa ttgcgcggga tgattttttc cgccaagccg
      781 ccgtaggttt ccaaaccgag cgacaaaggt gtaacgtcca gcagcagcca ttcgccgtcg
      841 gttttgttgc ctgcgaggac gtttgcctgt atggcggcgc cgagcgcgac gacttcgtcg
      901 ggattgaggt tgttcagcgg ggtttgtccg aagaaggtgg cgactgcctg ttggacgtgc
      961 agcatacggg tcgaaccgcc gaccataatc acgcctttga cttcgttttt accgacaccg
     1021 gcatctttca acgcctgtgt gaccggttcg agcgttttca tcaccaaatg ctgcgtcagg
     1081 ttgtggaact cggcgcgact gatgcttgtg tcgattggca tgccgtctga aagcgtcgcc
     1141 tgaatgcgcg cttcggtttg cgtggttaat tgttctttgg cggcgcggac gagcgagagc
     1201 aggagttggc tgtcttgttc gttgagttgg gagagtccgt tttgttcgag caggcggcag
     1261 aacaggcggt ggtcgaaatc gtcgccgccc aacgcgctgt tgccgccggt ggctttgact
     1321 tcaaacagtc ctttggtcag ttgcaatacg gatacgtcga atgtgccgcc ccctaagtcg
     1381 tacacgacaa acgtgccttc cgaggcgttg tccagcccgt aggcgattgc ggcggcggtg
     1441 ggttcgttga gcaggcgcaa tacgttcaaa cccgccagac gcgcggcatc tttggtggcc
     1501 tggcgttggg catcgtcgaa ataggcgggg acggtaatca ccacgccgac caaatcgccg
     1561 cccaaggttt cttcggcgcg cgatttaagg gttttgagga tttccgccga cacttcgaca
     1621 ggcgttttca ccccctgccg cgtatgcagt tcgataacgc gttgattgcc gccgaaacgg
     1681 taaggcaggt agtgcgtatt ttgatgcaga tcggcaagcg tccgcccgat aaggcgtttg
     1741 gcggagctga cggtgttcag cgggtcggtt ttttgggcgg acagggcggt tttgccgact
     1801 tcaatgccgc cgttttccag atagcggacg acggaaggca gggtaacgcg cccttcggca
     1861 tcgggcaggc aggcggcact gccgctgcgg acggtggcga ccaagctgtt ggtcgtaccc
     1921 aaatcgatgc ctgccgccaa acggtgccgg tgcggggcgg cggacatacc cggttctgaa
     1981 atctgcaaaa gagccataaa ttctgcctgt cggtgcggtg tgtaaaggaa ggaaagcggc
     2041 attttaacag aatttgcgcc gatagttgag caaaccgcta aggattaggt cgtgccgccg
     2101 aaattgccct ctaccgccaa atgccgataa aatccgcctt tcccgcccat cattaggaat
     2161 ataaatcgtg aaaggactcg actattgccg ccaaaaagca gaagaaagcc gctccagttt
     2221 tttgtcgggc ttccgtttcc tgactcagga aaaacgggat gcggtaacgg ttttatatgc
     2281 tttttgccgc gaattggacg atgtggttga cgaatgttcc aaccccgatg ttgcacaggc
     2341 gacattgaac tggtggcgcg gcgatttgga caaggtattc ggcggcgcga tgccggaaca
     2401 ccccgtcaat caggccttgc ggcaagttaa ggaaaccttc aagctgccga aatatgaact
     2461 ggaagcctta atcgacggga tgcagatgga tttggttcaa gcccgttacg gcagttttga
     2521 agaattgaaa ctgtattgcc accgcgtcgc aggcgtggtc ggctgcctga ttgcgcggat
     2581 tttggggttt tcagacgacc aaacgctgga atacgccgac aagatgggac ttgcgctgca
     2641 actgaccaac atcatccgcg atgtcggcga agacgcgcgc aggggacgga tttacctgcc
     2701 gatggaggaa atgcggcggt ttgacgtacc cgcaagcgtg attttgcaat gcagcccgac
     2761 agaaaatttc gccaaactga tggcgttcca aatcgaacgc gcccgcgaaa cctactgcga
     2821 agccgtatcg ctgctgccgg ctgccgataa aaaagcccaa aaagtcggac tggttatggc
     2881 ggcggtttat tacgagctat tgaacgaaat cgaccgggat ggcgcacaaa acgtcctcaa
     2941 atacaaaatc gccctccctt cgccgcgcaa aaaacgcatt gccctgaaaa cctggttatt
     3001 cggattcaaa ccgcgccccg gcacgccgga acgggcataa ggcgcatacc gcccgccctt
     3061 caggaaaacc caacccccgc cgccccgaaa aacggggttt caaccattgg aggaataaat
     3121 gatgaacacg ccgcatccgc gcccgaaaat cgccgtcatc ggcgcaggct gggcaggact
     3181 gtccgccgcc gtcaccttgg cgcggcacgc cgacgttacc ctgtttgaag ccggccggca
     3241 ggcgggcggc agggcgcgcg cactggccgg aaataccgac ggtttcggtt ttttggacaa
     3301 cgggcagcac attttgctcg gcgcataccg gggcgtgttg cgcctgatga aaaccatcgg
     3361 ttcggatccc cgtgccgcct ttttgcgcgt accgctgcac tggcatatgc acggcggttt
     3421 gcagttccgc gccctccccc tgcccgcgcc gctgcatatt ttgggcggcg tgctgcttgc
     3481 ccggcgtgca ccgactgcat tcaaagccaa actgcttgcc gatatgtccg atttgcagaa
     3541 gtccgcacgc ctcggacagc ccgacacgac agtggcgcaa tggctgaaac agcggaacgt
     3601 gccgcgtgcc gccgtgatgc agttttggca gcccttggtt tggggcgcgc tcaacacgcc
     3661 tttggaaacc gcaagcctgc gcgtgttgtg caacgttttg tccgacggcg tgctgacgaa
     3721 aaaatccggc agcgactatc tcctacccaa gcaggatttg ggcgcaatcg tcgccgaacc
     3781 cgccttggcg gatcttcaac ggctcggcgc ggacatccgc ctcgaaacgc gcgtatgccg
     3841 tctgaacacc ctcccggacg ggaaagtgct cgtcaacggc gaagctttcg atgccgccgt
     3901 ccccgccacc gcgccctacc acgccgccgc gctcctgccc gaaggcacgc ccgaacacgt
     3961 tcagacggca tatcaaaacc ttcgctacca cgccatcacc accgtctatc tgcgctacgc
     4021 cgaacccgtc cgcctgcccg ccccgctgac cggccttgcc gacggcacgg tgcaatggct
     4081 gctttgccgg ggcaggctcg gactgcctga aaacgaagtg tccgccgtca tcagcgtttc
     4141 cgaccgcgtc ggcgcgttta caaaccgagc gtgggcggac aaagttcacg ccgacctcaa
     4201 acgcatcctt ccgcatttgg gcgaacccga agccgtgcgc gtcatcaccg aaaaacgcgc
     4261 cacaaccgca gccgatgccc cgccgccgga tttgtcgtgg ctgcaccggc accgcatctt
     4321 ccccgccggc gactacctcc acccggacta ccccgccacg ctcgaagccg ccgtacaatc
     4381 aggtttcgcg gcggcggaaa cctgcctgca aagcctgagc gatgccgtct gaaaacgccg
     4441 gccggcatcg ggacgcttta caaggtgcgg caaaacgcta aaataccccc gaccgaccac
     4501 caaaaaggaa aaagtatggc gacactgtcc gacaaaacca tcttaattac cggcgcatcg
     4561 caaggtctgg gcgaacaggt cgccaaagcc tatgcggcgg caggcgcaac cgtgattctg
     4621 gtggcacgcc atcagaaaaa actggaaaaa gtctatgacg cgattgtcga agccggacac
     4681 cccgaaccct tcgccatccg tttcgacctg atgagtgcgg aagaaaaaga attcaatcag
     4741 ttcgccgcca ccattgccga agccacgcaa ggcaaactgg acggcatcgt ccactgcgcc
     4801 ggctattttt acgccctctc gccgctggat ttccaaaccg tcgccgaatg ggtcaaccaa
     4861 taccgcatca acaccgtcgc acctatgggg ctgacccgcg ccctgttccc gctgctgaag
     4921 cagtcgcccg acgcgtccgt catcttcgtc ggcgaaagcc acggcgaaac acccaaagcc
     4981 tactggggcg gcttcggcgc gtccaaagcc gcgttgaact acctgtgcaa agtcgccgcc
     5041 gacgaatggg aacgcttcgg caacctgcgc gccaacgtcc tcgtccccgg ccccatcaat
     5101 tccccgcaac gcatcaaatc ccatccgggc gaagccaaaa gcgaacgcaa aagctacggg
     5161 gacgtgctgc ccgcatttgt ctggtgggca agtgccgaaa gcaaagggcg gagcggcgaa
     5221 atcgtttacc tctaaatccg gaaaaggcag gcatcccgcc cgcgccgcga ttccgtttga
     5281 aaaatatccg tacctttcgc gcgcatccgt cccgcacaga caaacacctt gacttaaatt
     5341 tacataagga caataatgat aacccgttat ttccttaact tgaccttagg gtaaatttta
     5401 tgaaaaccgt ttccaccgcc gttgcccttg ccgccgcttc cgtcttactg tcggcctgtg
     5461 catccgaatc ttcacgcagc ctcgaagttg ccacagtcgc ttcttacagc acccaatacc
     5521 acggcgtgcg taccccgatt tccgtcggaa cattcgacaa ccgctccagc ttccaaaaag
     5581 gcattttctc cgacggggaa gaccgtttgg gcagccaggc aaaaaccatt ctggtaacgc
     5641 acctgcaaca gaccaaccgc ttcaacgtac tgaaccgcac caatttgaac gcattaaaac
     5701 aggaatccgg catttccggc aaagcgcata acctgaaagg cgcagattat gtcgttaccg
     5761 gcgatgtaac cgaattcgga cgcagagatg tcggcgatca tcagctcttc ggcattttgg
     5821 gtcgcggcaa atcgcaaatc gcctatgcaa aagtggctct gaatatcgtc aacgtcaata
     5881 cttccgaaat cgtctattcc gcacagggcg cgggcgaata cgcactttcc aaccgtaaaa
     5941 tcatcggttt cggcggcact tccggctacg atgcgacttt gaacggcaaa gttttagact
     6001 tggcaatccg cgaagccgtc aacagcctgg ttcaggctgt tgacaacggc gcatggcaac
     6061 ccaaccgtta aggagaaatg atgaatccga aaaccttgag ccgtttgtcg ctgtgtgcgg
     6121 cggtcttggc tctgaccgcc tgcggcggca acgggcaaaa atccctgtat tattacggcg
     6181 gctatcccga taccgtctat gaaggtttga aaaacgacga cacttcgttg ggcaagcaga
     6241 ccgaaaagat ggaaaaatac tttgtggaag ccggcaacaa aaaaatgaat gccgccccgg
     6301 gtgcgcacgc ccatctggga ctgctgcttt cccgttcggg agacaaagag ggcgcgttcc
     6361 gccagtttga agaagagaaa aggctgtttc ccgaatcggg cgtatttatg gacttcctga
     6421 tgaaaacagg taaaggaggc aagcgatgaa acctttgatt ttagggcttg ccgccgtgtt
     6481 ggcgctgtct gcctgccaag ttcaaaaagc gcccgatttc gactacacgt cattcaagga
     6541 aagcaaaccg gcttcaattt tggtggttcc gccgctgaac gaatcgcccg atgtcaacgg
     6601 cacttggggg atgctggctt cgaccgccgc gccgctttcc gaagccggct attacgtctt
     6661 ccctgccgca gtcgtggagg aaaccttcaa acaaaacggc ttgaccaatg ccgccgatat
     6721 tcacgccgtc cggccggaaa aactgcatca gattttcggc aatgatgcgg ttttgtacat
     6781 tacggttacc gaatacggca cttcatatca aattttagac agcgtgacga ccgtatccgc
     6841 caaagcacgg ctggtcgatt cccgcaacgg aaaagagttg tggtcgggtt cggccagcat
     6901 ccgcgaaggc agcaacaaca gcaacagcgg cctgttgggg gctttggtcg gcgcagtggt
     6961 caatcagatt gccaacagcc tgaccgaccg cggttatcag gtttctaaaa ccgccgcata
     7021 caacctgctg tcgccctatt ctcacaacgg catcttgaaa ggtccgagat tcgttgaaga
     7081 gcagcccaaa taagcatatg ccgtctgaac ctctccgagc ttcagacggc atatgcgaat
     7141 cttgatactt gcaagccgca ccgcccaaaa tcaggcagcc ggtttcaata tccgaacaag
     7201 gttcgggttc tgtattttgc agaacccgaa ccttttattc aaatttaaac ttaattccgg
     7261 cgtggttgta
//
LOCUS       DAXHKX010000100         7245 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHKX010000100 DAXHKX010000000
VERSION     DAXHKX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.100"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            191..1726
                     /locus_tag="ACFMWZ_001464"
     CDS             191..1726
                     /locus_tag="ACFMWZ_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC5935413.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="ACFMWZ_001465"
     CDS             1723..1815
                     /locus_tag="ACFMWZ_001465"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC5935414.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="ACFMWZ_001466"
     CDS             complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="ACFMWZ_001466"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC5935415.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRINPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVEPKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3106..3276)
                     /locus_tag="ACFMWZ_001467"
     CDS             complement(3106..3276)
                     /locus_tag="ACFMWZ_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC5935416.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFMWZ_001468"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFMWZ_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC5935417.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            3725..4231
                     /gene="luxS"
                     /locus_tag="ACFMWZ_001469"
     CDS             3725..4231
                     /gene="luxS"
                     /locus_tag="ACFMWZ_001469"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC5935418.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            4377..7172
                     /gene="polA"
                     /locus_tag="ACFMWZ_001470"
     CDS             4377..7172
                     /gene="polA"
                     /locus_tag="ACFMWZ_001470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC5935419.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         1649 a         1950 c         1986 g         1660 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc taaggtttcg
     1921 cgtcggcgga tgagtttgta tccgccaccg ttaaccaaca cttccgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggctccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcacaaatc
     2101 gggccgacga tgttggcagt cagcggcgcg atgttttttg gttctaccgc ttcgatgtga
     2161 tgataggcat catatagggc ggggcgcatt aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcaccgtg tttgacgaat tcgacacgtg tcagcagcga acctgcgttg
     2281 ccgaccaagc tgcggccggg ctcaagaatg agtttcagac ggcgtgtacc gatcagtttt
     2341 tgaactgctt gggcatacgc gcccaaatca ggcacttttt cgtcttggta aacaatgccg
     2401 acgccgccgc ctaagtccaa atgttctaaa acaatgcctt cggcggcaag agcgtcaacc
     2461 aaaatcaaaa tgcgttcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcgtg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagacctgt ggagatgtag
     2641 ggatgggttt ttgcatcgac atcggggttg atgcgcaggg aaacggacgc ggttttgccc
     2701 aaacgcgcgg caactttttg aatacggtcg atttcgggaa tgctttccat attgaagcat
     2761 ttcacgccgg cattcagcgc gaactcgatt tctgcctcgc ttttgcccac gcctgaaaat
     2821 atggtttttg ccgcatcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctgcccaa agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaacagg gtcatatttt cgttttcatt tttgggtttg
     3121 tggagcggat tgcggtttgc tttgaagctg caaaccggtt tggattacgc cgaaacgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggtat cttaaacaaa aaacacgcaa aaagctatga tgaccgaaag
     3361 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
     3421 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccggagacgg
     3481 cacgcaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtggattg ccgcaaaaag
     3541 cggcggctac catttcgccg agcaaaacgg caaatggctg gcaacgcgcg acggacgcga
     3601 tttttacgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg tggaaattgc
     3661 ggaattgtga ttcagtattc aaatatccct agttaagaat ctattgacct taaaggataa
     3721 cccaatgccc ctactagaca gtttcaaagt cgatcacacc cgtatgcatg cccccgccgt
     3781 acgcgtggcg aaaaccatga ctacgcccaa aggcgacacc attaccgtgt ttgacctgcg
     3841 cttttgcgtt cccaataaag aaatcctgcc tgaaaaaggc atacacacgc tggagcattt
     3901 gttcgccggc tttatgcgcg accacttgaa cggaaacggc gtggaaatca tcgacatttc
     3961 cccgatgggc tgccgcaccg gtttttatat gagccttatc ggcacgcctt ccgaacagca
     4021 ggtcgccgat gcgtggctgg cttcgatgca ggatgttttg aatgtcaaag accaaagcaa
     4081 aatccccgag ttgaacgaat accaatgcgg tacttacctg atgcactcgc ttgccgaagc
     4141 gcaacaaatc gcgcaaaacg tgttggcgcg caaagtggcg gtgaacaaaa atgaagagct
     4201 gacgctggat gaagggctgc tgaacgccta atccgccaaa aatgccgtct gaacaagggt
     4261 ttcagacggc atttgccttt tccgttataa tccggggttg tccgggggcg ggttttaagc
     4321 cggcatcgtc cttccctatt tttttctgtc ccttatcggt tttaagcggg ttttttatgt
     4381 ccaacagacc tacactcctc ctcgttgacg gatcgtccta cctctaccgt gcgtatcacg
     4441 cgatggggca aaacctgacc gcccccgacg gcgcgccgac gggtgcgctg tatggtgtat
     4501 tgaatatgtt gcgccgtttg cggtcggaat atccgcacga ttattgcgcg gtggtttttg
     4561 atgcgaaagg caaaaatttc cgccatcaaa tgtttgaaga atacaaggcg acgcgcccgc
     4621 cgatgcccga cgatttgcgt ccgcaggcgg aagcactgcc ggatttagtg cgcctgacgg
     4681 gctggccggt attggtgatt gggcaagtgg aggcggacga tgtgatcggc acgctggcga
     4741 aacagggggc ggaacatggt ttgcgagtca ttgtttcgac cggcgataag gacatggcgc
     4801 agttggtgga tgagcgcgtt acgctggtga acacgatgag cggagaaacg ctggatattg
     4861 aaggcgtgaa ggcaaaattc ggcgtgcgcc ccgaccaaat ccgcgattat ctcgcgctga
     4921 tgggcgataa ggtggacaac gtgccgggcg tggaaaaatg cggcccgaaa acggcggtga
     4981 agtggctgga agcctacggc tcgttgcaag gcgtgataga acacgcttcg gaaatcaagg
     5041 gcaaagtggg cgaaaacctg caagccgcgc tgccccaact gccgctgtcg tatgatttgg
     5101 tcacgattaa aaccgatgtg gacttgcacg ccgagctttc agacggcatc gaaagcctgc
     5161 gccgtaccgc gccgaaatgg gcgcaactgg ttgtcgattt caaacgctgg ggcttccgca
     5221 cctggctgaa agaagcggaa tcaaacatga ataccggctc gaccgatgat ttgttcggca
     5281 gcgacagcat cggcgagcag gcggctttga atgcggaaat gccgtttgaa aaacaagccg
     5341 aaaaagccac cgcccccgaa aaactggatt atcaagccgt taccaccgaa gcgcagtttg
     5401 ccgctttgtt ggacaaactg tcgcgggcgg acacaatcgg catcgatacg gaaaccacgt
     5461 cattagacgc gatgaacgcc tcgctggtcg gcatcagcat cgctttccaa gcaggcgaag
     5521 cggtttacat ccccgtagga cacagcctga ccgccgcacc cgaacagctt gatttgcaag
     5581 acgtgttagg ccgtctgaaa ccgcatttgg aaaaccccgc cctgaaaaaa atcgggcaaa
     5641 acctcaaata tgaccaacat gttttcgcca attacggtat cgccctgaac ggcatcgccg
     5701 gcgacgccat gctcgcttcc tacatcattg agagccatct cggacacggc ttggacgaat
     5761 tgtccgaacg ctggctgggc ttggaaacca ttacctacga atcgttgtgc ggtaaaggcg
     5821 cgaagcaaat cggttttgcc gatgtcgcca tcgggcaggc gaccgaatac gccgcccaag
     5881 acgccgattt cgccctgcgc ctcgaagcgc acctgcgcgc acaaatggac gccaaacagc
     5941 ttgaaatgta tgagaaaatg gagctgcccg tcgcgcaggt attgtttgaa atggaacgca
     6001 acggcgtgca aatcgaccgc gccgaactcg cccgccaaag cgcagaactc ggcgccgagc
     6061 tgatgaagct tgaacaagaa gcctatgccg ccgcaggaca gccgttcaac ctcaactcgc
     6121 ccaaacagct tcaagaaatc ctgttcgaca aaatgggcat ccccaccaaa ggcctgaaaa
     6181 aaaccgccaa aggcggcatt tccaccaacg aagccgtgct cgaacagctc gcgcccgact
     6241 accccctgcc caaaatcatc ctgcaaaacc gcagcctggc aaaactcaaa tccacctaca
     6301 ccgacaaact gcccgaaatg atttccccca aagacggccg cgtgcatacc acctacgccc
     6361 aagccgtcgc cattacagga cgcttggcaa gcaacaaccc caacctgcaa aacatcccca
     6421 tccgcaccgc cgaaggccga cgcgtgcgcc gcgccttcac cgcaccgcaa ggcagcgtca
     6481 tcgtttccgc cgactattcc caaatcgagc tgcgcatcat ggcgcacctc tccggcgaca
     6541 aaaccctcat tgccgcgttc caaaacggcg aagacgtaca ccgccgcacc gccgccgaag
     6601 tgttcggcat cgcccccgaa aacgtctcgt ccgaacaacg ccgctacgcc aaaaccatca
     6661 acttcggcct gatttacggc atgggccaat acggccttgc caaatcattg ggcatcgaca
     6721 acatctccgc taaaaacttt atcgaccgct acttcgcccg ctaccccggc gtcgccgaat
     6781 acatgcagcg caccaaagaa caagccgccg cccaaggctt cgtcgaaacc ctgtttggcc
     6841 gccgcctcta cctgcccgac atccgcaaca aaaacgccaa cgcccgcgcc ggagccgaac
     6901 gcgccgccat caacgccccc atgcaaggca ccgcctccga ccttatcaaa cgcgccatga
     6961 tagacgtgtc ccgctggctg gtttcagacg acctgaagag caaactgatt atgcaggtgc
     7021 atgacgaact ggtgctggaa gtcgttgaaa ccgaactgga ttttgtcaaa gaaaaactgc
     7081 cgcagattat ggcgaaagtg ggcggcggat tattggatgt accgctggtg gctgaggttg
     7141 gcgtagggga gaattgggaa gaggcacatt gattgaaagg tgttattatg ctatctttat
     7201 ttaaataaaa tttaattttt ggtatatttt ttctaaatgt tccta
//
LOCUS       DAXHKX010000101         7121 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHKX010000101 DAXHKX010000000
VERSION     DAXHKX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.101"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(100..459)
                     /gene="rnpB"
                     /locus_tag="ACFMWZ_001471"
     ncRNA           complement(100..459)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFMWZ_001471"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(538..1641)
                     /gene="prfB"
                     /locus_tag="ACFMWZ_001472"
     CDS             complement(538..1641)
                     /gene="prfB"
                     /locus_tag="ACFMWZ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC5935420.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1744..2637)
                     /locus_tag="ACFMWZ_001473"
     CDS             complement(1744..2637)
                     /locus_tag="ACFMWZ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC5935421.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIVKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2979..3428
                     /locus_tag="ACFMWZ_001474"
     CDS             2979..3428
                     /locus_tag="ACFMWZ_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC5935422.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3502..3810
                     /locus_tag="ACFMWZ_001475"
     CDS             3502..3810
                     /locus_tag="ACFMWZ_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935423.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3926..4768
                     /locus_tag="ACFMWZ_001476"
     CDS             3926..4768
                     /locus_tag="ACFMWZ_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC5935424.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4758..5504
                     /locus_tag="ACFMWZ_001477"
     CDS             4758..5504
                     /locus_tag="ACFMWZ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC5935425.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDACAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGFSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEDNSPGQNHLSA"
     gene            5526..6113
                     /locus_tag="ACFMWZ_001478"
     CDS             5526..6113
                     /locus_tag="ACFMWZ_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC5935426.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6119..6298
                     /locus_tag="ACFMWZ_001479"
     CDS             6119..6298
                     /locus_tag="ACFMWZ_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC5935427.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6530..6970
                     /locus_tag="ACFMWZ_001480"
     CDS             6530..6970
                     /locus_tag="ACFMWZ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC5935428.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1809 a         1893 c         1628 g         1791 t
ORIGIN      
        1 ccgtaccggt ttttgttaat ccactataaa aacacaacct aaataaaaat gccgtctgaa
       61 ccatatttca ggtttcagac ggcatttgcg tgtcggatgc acaccggacg ggcggtaagc
      121 cgggttctgt ctcggacagt cattcctcta ggcataccgt taccggtatg ctcaagcaac
      181 ctacccgaac gctcggcggg cagcgtcatt gcgttctgtt tggtcttgct ccgaatgggg
      241 tttggcctgc cgcatattgt taccaaatgc gcggtgcgcc cttaccgcac cttttcaccc
      301 ttgcctgtgc tgccaaagca gccatcggcg gttttgcttt ctgttccact ttccgtcgcg
      361 ttaccgcgcc cggccgttaa ccggcatcct accctgcgga gcccggactt tcctccccgt
      421 atgccttacg cgatacgcgg cgactgtctg cccgtcccgt gtgcggcgcg gattataaca
      481 cgaaacgcaa aaatgccgtc tgaaacggta caggtttcag acggcataca gcctaaacta
      541 cacgccctgt ttcaggctgg cttcgataaa gccgtccaaa tcgccatcca atacggcttt
      601 ggtgttgccg acttcgtagc ctgtacgcaa gtctttgata cgtgaggaat ccaaaacata
      661 cgaacggatt tggctgcccc aacctacatc ggatttacct tcttccaacg cctgtttctc
      721 ttcattgcgt ttgcgcattt ccaattcata cagtttggac ttcaacattt ccatcgcggc
      781 ggctttgttg gcgtgttgcg aacggtcgtt ttgacattgc accacaatcc ccgtcggctc
      841 gtgggtaatg cgcacggcgg agtcggtttt attgatgtgc tgaccgcccg cacccgatgc
      901 gcgataggtg tcgatgcgca aatcggcggg gttgatttcg atttcgatgg aatcgtcgat
      961 ttcgggatag acgaacacgg aggcaaacga ggtatggcgt ttgttgttcg agtcaaacgg
     1021 agagtaacgc accaagcggt gaacacctgt ttccgtgcgc agcaagccgt aggcatattc
     1081 gccttccact cggatagtcg cgcggttgat gcccgcgatt tcgccgtcgt cttcttcgag
     1141 gatttcgatt ttgaagcctt tgcgctcggc gtagcggctg tacatgcgga acagcatacc
     1201 cgcccagtct tccgcttccg taccgcctgc gcctgcggta atgtcgataa agcagttgtt
     1261 cggatcggcg ggctggttga acatccgttt gaactccaaa tccgccatct gtttttccag
     1321 ccccgccaca tcttcctgca cggcggcaaa accttcttcg tcgttttctt cgacggtcat
     1381 ttcaatcagc atgcggttgt cttcgatgcc cgaagcgatg ttgtcgagcg tcaacacgat
     1441 gccttcgagg attttgcgct ctttgccgat ttcttgggcg cgtttcgggt cgttccaaag
     1501 ttcggggtct tcggaaagac cgacaacttc ttccaatcgg tctttcttac cctgataatc
     1561 catataaact cggatgtctt cgctgcgctt ttccaaatca ttcagggtat tgttgagctg
     1621 gttgattact tcggcttcca tgattctttt gttctttcaa aattttaggg gcgtattgta
     1681 cgggattcgg gtattttttt ctatggataa agccttctgg aaacacgttc ggacggcata
     1741 gcgtcaataa cggtatgccg ccagtttgcg tttgatttca ggcaatgcgg cacgtgctgc
     1801 ctcctcaccc aactggatgg cacgtttttt ctgatcgaaa ccgccgactg cacccaaatc
     1861 caaaacctgc ggtttgataa ccacatccgc ctgccccaac tcattttgca acgcggaaac
     1921 gctcattacg ttcagcgtct gatcgagata agagaagaag ccttggctga tgtttttgcc
     1981 cggacgggcg gaaatatcga cggcaatcac gaaattcgcc ccctgccgcc gggcggcact
     2041 gacgggcacg ggctgcgaca gaccgccgtc aacatatgta tgcctgccga tgataacggg
     2101 ttggaacaca ttgggaatgg cggcggaagc gcgcacagcc tgcccggcat tcccttgatt
     2161 gaaagcgacg gccttgccgg tttcaaaatc agtagcaaca gcggcaaatt tgatgggaaa
     2221 ctgctgaatc tgcctgccgc cgacttttcg gttgatgtaa ttttgcagct tttcgccttt
     2281 gataaaacca ctggtggaca aggttaaatc gactaaatcg gttttgccta aaatttcggc
     2341 ttccaattcg aggcggtcgg gcgacatacc cgatgcaaaa aggctgccga caatcgaacc
     2401 tgccgatgtg ccggtaacca ccttcacagg aataccgttt tctttcaaaa ccttaacaat
     2461 acctacatgg gcaaatcctt tagatgcgcc gccaccgagt gccaaaccga ccactgcggc
     2521 gggtttggcg gtttgcaccg gcttgcgggc agcattattt cccgccgtgc cgcaggcggc
     2581 aagcaacgcg gcggcggcga ttgccaaaag cggtctgatt tttgaaaacg ttaccatatt
     2641 ttccattcct ttatatatcg caccccgtca aaaagaggga ttgcttttct taacaacccc
     2701 ctttgacagc caagcaaatg ggggctttgt taagtcatca tcaaaattaa tatttctttt
     2761 tttcctttac ggaaattata tttgaaggca tactatccaa ggcgggaatt atctcacaac
     2821 accgccgtta tccaaatatc ccgccttttt cctttccttt ccatcaaaat actttctttt
     2881 tatattcatt aacttgttaa atcattggct gccgggtgtc agtttttccg acaaaatccg
     2941 tctaatgggg tatcaacaga accaaaacag gaacacttat gaaaatcgga acaacttggc
     3001 agacggcatc cgctatgctg gttttgcgtc tgtttgccgc atatgaattt ttggaatcgg
     3061 gtttgcaaaa atggaacggg gagaattggt tttccgaaat caacgatcag tttccattcc
     3121 cgttcaactt gctgccggac gcgttaaact ggaatctcgc tatgtatgcg gagcttttac
     3181 tgcccgtact gttgcttttg ggtttggcaa cgcgtctgtc ggcattaggg ctgatggtcg
     3241 ttaccgccgt cgcttgggct gcggttcacg ccggttcggg ttacaatgtc tgcgacaacg
     3301 gttataaaat ggctttaatt tatatcgtgg tattaatccc gctgcttttc cagggtgcgg
     3361 gcggatggtc gctggatacg ctgctgaaaa aacggttttg cccccaatgc cgtctgaagc
     3421 aagattgatt cagtcgtgga atctgacttt aaacattcca accttatctc gttaacttga
     3481 tattctgaaa aggaaataga aatgaacaaa aacattgctg ccgcactcgc cggtgcttta
     3541 tccctgtctt tggccgccgg tgcagttgct gccaacaaac cggcaagcaa cgcaacaggc
     3601 gttcataaat ccgcccatgg ctcttgcggc gcgtccaaat ctgccgaagg ttcgtgcggc
     3661 gcggctggtt ctaaagcagg cgaaggcaaa tgcggcgagg gcaaatgcgg tgcgaccgta
     3721 aaaaaaaccc acaaacacac caaagcatct aaagccaagg ccaaatctgc cgaaggcaaa
     3781 tgcggcgaag gcaaatgcgg ttctaaataa tcccacccct tcaaaccaag ccgcgttttt
     3841 cagtaaaatg cggctttttt aacggcaaac aaagattttt taacaagcac atcattcttt
     3901 tgtgccatcc gaaccgggta aaaatatgat tcaacacgca ggcttgggct accgccgcga
     3961 cttggcggaa gactttctct cgctttccga aaacagcccg atatgcttta tcgaagccgc
     4021 accggaaaac tggctgaaaa tgggcggctg ggcgcgcaaa cagtttgacc gtgtggcgga
     4081 acggctgccg ctggcgttgc acggattgtc tatgtcgctg ggcgggcaag caccgctgga
     4141 tactgatttg atagacggca tcaaagaaat gatgcgccgt tacgattgca cgtttttctc
     4201 cgaccatttg agctactgcc acgacggcgg tcatctttac gatttgttgc cgctgccctt
     4261 taccgaggaa atggtgcatc atacggcgcg gcgtatccgc gaagtgcaag accgtttggg
     4321 ctgccgcatc gccgtggaaa acacgtccta ctatctgcat tccccgcttg ccgagatgaa
     4381 cgaggtcgag ttcctcaacg cggttgcacg tgaggccgat tgcggcattc atctggacgt
     4441 gaacaatatc tacgtcaacg ccgtcaatca cggcctgttg tcgccggagg cttttttgaa
     4501 aaacgttgac gcagatcgcg tgtgctatat ccatattgcc ggacatgacg tggaaacgcc
     4561 ggaattgttg attgatacac atggcgcggc agttttgccg actgtttggg acttgctcga
     4621 acttgcctat gccaagctgc cgacgattcc gcccaccctg ttggaacgcg attttaattt
     4681 cccgcctttt gccgaactcg aagccgaagt cgccaaaatc gccgattatc aaacgcgtgc
     4741 cggaaaggaa taccgccgtg cagcctgaaa cctccgccca ataccagcac cgtttcgccc
     4801 aagccatacg cgggggcgaa gccgcagacg gtctgccgca agaccgactg aacgtctata
     4861 tccgcctgat acgcaacaat atccacagct ttatcgaccg ctgctatacc gaaacgcggc
     4921 aatactttga cagcgaagaa tggagccgtc tgaaagaagg tttcgtccgc gacgcgtgcg
     4981 cccaaacgcc ctattttcaa gaaatccccg gcgagttcct ccaatattgc caaagcctgc
     5041 cgcttttaga cggcattttg gcactgatgg attttgaata tacccaattg ctggcagaag
     5101 ttgctcaaat tccggatatt cccgacattc attattcaaa tgacagcaaa tacacacctt
     5161 cccctgcggc ctttatccgg caatatcgat atgatgttac cgatgatttg catgaagcgg
     5221 aaacagcctt gttaatatgg cgaaacgccg aagatgatgt gatgtaccaa acattggacg
     5281 gcttcgatat gatgctgcta gaaataatgg gtttctccgc gctttcgttt gacaccctcg
     5341 cccaaaccct tgtcgaattt atgcctgagg acgataattg gaaaaatatt ttgcttggga
     5401 aatggtcagg ctggactgaa caaaggatta tcatcccctc cttgtccgcc atatccgaaa
     5461 atatggaaga caattccccg ggccaaaacc atctatccgc ataaaattac cttgttcccg
     5521 atactatgcc gctacccgac ctgaccgatg ccgaattaat agagtcgcgt aaactgcttc
     5581 tgcattttgc gcggcttcag ttgcccgacc accctgattt ggctgaagat ttagtgcagg
     5641 aaacattgct gtccgcatac agcgcaggcg acagttttca aggcagggcg cttgtcaaca
     5701 gctggctttt tgccatattg aaaaacaaaa ttattgacgc attacgtcaa atcggaaggc
     5761 agaggaaagt ctttaccaca ctggatgacg agctactgga tgaagcattt gaaagccatt
     5821 tttcccaaaa cgggcattgg acgccggaag ggcagccgca acattggaac actccggaaa
     5881 aatcattaaa caacaacgaa ttccaaaaaa ttctgcaaag ctgcctatac aacttgcctg
     5941 aaaacaccgc acgggtattt accctgaagg aaatactcgg tttttcatcc gacgaaatac
     6001 aacaaatgtg cggtatcagc acgtccaact accacaccat tatgcaccgc gcccgagaat
     6061 cattgcgcca atgcctgcaa atcaaatggt tcaaccaaga aaacccgaag taaacgttat
     6121 gaaaaaatgc cgcgatatcg ccctgcttct ttccaaacat caggaccggg aaaccacccc
     6181 gggcgagaag atttccatat acacacacct gctgttctgt ccgtattgcc gtgaatataa
     6241 aagacaactt caaaccatca aaagatcact ggcaaaaaca accagaactt caaaataaat
     6301 gccgtctgaa aaggcttcag acggcataag ctgacggaaa caaatcaaac cgatttactg
     6361 ttatcggcag ttcatccata atacacgctt caaaagcagc atattcctcc atacggaata
     6421 tataaatacg caaaacacga aggctgcatc aatttgcttt atttgcctta tttcacagac
     6481 ggcgctaccc ctcccgccca acccgttctt tctgaatgag cagatttcaa tgattaagga
     6541 aaccctaatg cgcccaatct tcctatcttt cgttttactc cctattttga taaccgcctg
     6601 cagcacaccg gacaagtctg cacgatggga aaatatcggc acaatctcaa acggcaatat
     6661 tcatacatat atcaataaag acagcgtgag aaaaaacgga aatctgatga ttttccaaga
     6721 taaaaaagtt gttaccaatc taaaacaaga acgttttgcc aacacccccg catacaagac
     6781 tgccattgcc gagtgggaaa tccactgcaa caacaaaaca taccgcttaa gttcgctaca
     6841 gttgtttgat acaaaaaaca cggaaatttc cacacaaagc tacacagcct cttccctccg
     6901 cccgatgagc atcctgtccg ggacattaac cgaaaaacaa tatgaaaccg tatgcggaaa
     6961 aaaactctga ttgcaactta tacacaaact tacccacaaa ccttatcata aaaatgccgt
     7021 ctgaaatact gaaatatcag catttcagac ggcattttgc cattccctga aaattatcca
     7081 caaagttatc cacattattt tttaaaaccg gcttccatcc g
//
LOCUS       DAXHKX010000102         7093 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHKX010000102 DAXHKX010000000
VERSION     DAXHKX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.102"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            781..933
                     /locus_tag="ACFMWZ_001481"
                     /pseudo
     CDS             781..933
                     /locus_tag="ACFMWZ_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     gene            1081..2823
                     /gene="hrpB"
                     /locus_tag="ACFMWZ_001482"
     CDS             1081..2823
                     /gene="hrpB"
                     /locus_tag="ACFMWZ_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HFC5935429.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWIRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     SYETAIHAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRH
                     QFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            2916..>7093
                     /gene="hrpA"
                     /locus_tag="ACFMWZ_001483"
     CDS             2916..>7093
                     /gene="hrpA"
                     /locus_tag="ACFMWZ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HFC5935430.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSVSMVLPAHAQIITDKTAPKNQQAVILKTNTGAPLVNIQ
                     TPNARGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQV"
BASE COUNT         2089 a         1725 c         1635 g         1644 t
ORIGIN      
        1 ttgtaaaagc aaggataggg catctgccct tttgattgtt tgattaacga tacaaggagt
       61 ttcaaaatga gagttttata gtggattaac aaaaaccagt acagcgttgc ctcgccttgc
      121 cgtaccattt gtactgtctg cggcttcgtc gccttgtcct gatttaaatt taatccacta
      181 tatgtgttca tgaaatgact tgggtcggag gctcaggtaa tgcgtaacaa agctcatatt
      241 attgcgaaat ttgcgaatct gcagggctta acgatacggg aaatcctgat aaatctttag
      301 gattgccaaa caatacgttc agtaatccgc ctggttgggg agctacaatc ggagctttag
      361 caggtagccg catagggatg cctgaatttg gtacgtttgc gagccatgcc attgaaaatt
      421 tcgactggtc atggtatcga cgttataggg aaattgccga aacgattgaa cgagaatatt
      481 caggcggttt gccttaatag ttgaggaggt catgatgttt gccaaacatt atcaattcat
      541 cgcactcggc atcatgctgc ttctttatat gttgattctc tatacgaccg atttttccaa
      601 tctgacgtat tggatgctgt tttttatctg ttttattaca ggaaaaatat tagctcgttt
      661 gttagagaaa agctttaaat aaaatagcag ctagtcgcaa aaggtcgtct gaaacctttt
      721 caggcggcct ttctaaaata catccaactt cctaatccct atttttcaaa aaggaaatct
      781 atgccccatc tgcaaaacct gtctttgggc ttaaagaaaa agctgcctgt tatcctgcaa
      841 acagaaatat cagaatgcgg cttggcatgt ctggcggctg tggcgggatt tcatggtttc
      901 catacggatt tacgcgcact gcgttcaaaa tactgtccga gacctttgca aaattcccca
      961 aaatccccta aatgtcttgg tgggaatttt ggggaatttt gcaaaggtct cattctataa
     1021 ctgtaaatac ttttaaattt atgacaaaat agtaaatatt gctaaaataa tattgatgtc
     1081 atgaaatttt ttcctgctcc atgtctgttg gttatcctgg ctgtcatacc ccttaaaacc
     1141 ttagctgccg atgaaaacga tgcagaactt atccgttcca tgcagcgtca gcagcacata
     1201 gatgctgaat tgttaactga tgcaaatgtc cgtttcgagc aaccattgga gaagaacaat
     1261 tatgtcctga gtgaagatga aacaccgtgt actcgggtaa attacattag tttagatgat
     1321 aagacggcgc gcaaattttc ttttcttcct tctgtgctca tgaaagaaac agcttttaaa
     1381 actgggatgt gtttaggttc caataatttg agcaggctac aaaaagctgc gcaacagata
     1441 ctgatcgtgc gtggctacct cacttcccaa gctattatcc aaccacagaa tatggattcg
     1501 ggaattctga aattacgggt atcagcaggc gaaatagggg atatccgcta tgaagaaaaa
     1561 cgggatggga agtctgccga aggcagtatt agtacattca ataacaaatt tcccttatat
     1621 aggaacaaaa ttctcaatct tcgcgatgta gagcagggct tggaaaacct gcgtcgtttg
     1681 tcgagtgtta aaacagatat tcagattata ccgtccgaag aagaaggcaa aagcgattta
     1741 cagatcaaat ggcagcagaa taaacccata cggttcagta tcggtataga tgatgcgggc
     1801 ggcaaaacga ccggcaaata tcaaggaaat gtcgctttat cgttcgataa ccctttgggc
     1861 ttaagcgatt tgttttatgt ttcatatgga cgcggtttgg tgcacaaaac ggacttgact
     1921 gatgccaccg gtacggaaac tgaaagcgga tccagaagtt acagcgtgca ttattcggtg
     1981 cccgtaaaaa aatggctgtt ttcttttaat cacaatggac atcgttacca cgaagcaacc
     2041 gaaggctatt ccgtcaatta cgattacaac ggcaaacaat atcagagcag cctggccgcc
     2101 gagcgcatgc tttggcgtaa caggtttcat aaaacttcag tcggaatgaa attatggata
     2161 cgccaaacct ataaatacat cgacgatgcc gaaatcgaag tgcaacgccg ccgctctgca
     2221 ggctgggaag ccgaattgcg ccaccgtgct tacctcaacc gctggcaact ggacggcaag
     2281 ttatcttaca aacggggtac cggtatgcgc caaagtatgc ccgcacctga agaaaacggc
     2341 ggcggtacta ttccaggcac atcccgtatg aaaatcataa ccgccggatt ggatgcagcg
     2401 gccccgttta tgttgggcaa acagcagttt tcctacgaaa ccgctatcca tgcacaatgg
     2461 aacaaaacgc ctttggttgc ccaagacaag ttgtctatcg gcagccgcta caccgttcga
     2521 ggatttgacg gtgaacagag cctttccgga gagcgaggtt tctactggca gaacactttg
     2581 ggctggcatt tccatccgcg ccatcaattc tatctcggcg cggactatgg tcgggtatcg
     2641 ggcgaaagtg cccaatatgc ctcgggcaag cggctggcgg gtgcagtggc cggcttcaga
     2701 ggcagccata aagtaggcgg tatatttgct tatgacttat ttgccggcaa gccgctgcac
     2761 aagcccgaag gttttcagac ggccaacacc gtttacggct tcaatttgaa ttacagtttc
     2821 taaccctctc ctgcattttt taccgacatt cagacagtct ttcatcattt acaggccgtc
     2881 tgaagcgatt acaaacatca tttaaaggga taaatatgaa caaaggtctg catcgtaccg
     2941 ttttcagcaa aaagcacaac actatggttg cagttgccga aaccgccaac agtcagggaa
     3001 aaggcaaaca ggcaggaact tccgctccgg cttcgccaca aatttcagac ggcctttaca
     3061 gtaaactcaa aaccacgctt aaaaccttgg tctgctcttt ggtttccgtc agtatggtat
     3121 tgcctgccca cgcacaaatc attaccgata aaaccgcgcc caaaaaccag caggccgtta
     3181 ttcttaaaac caataccggt gcgccgttgg tcaacatcca aactcctaat gcccgtggat
     3241 tgagccacaa ccgctatacc cagtttgatg tcgacgctaa aggtgctgta ctgaacaacg
     3301 atcgcaacaa caacccgttt ctggccaaag gcagtgcgca gttgattttg aacgaggtgc
     3361 gcggtgcggc cagcaaactc aatggcatcg ttaccgtagg tggtcaaaag gccgatgtga
     3421 ttatcgccaa ccccaacggc attaccgtga acggcggcgg ctttaaaaat gtcggccgcg
     3481 gtatcttaac taccggtgcg ccccaaatcg gcaaagacgg tgcactgaca ggatttgatg
     3541 tgcgtcaagg cacattgacc gtaggagcag caggttggaa tgataaaggc ggagccgact
     3601 acaccgagat acttgctcgt gcagttgctt tgcaggggaa attacagggt aaaaacctgg
     3661 cggtttctac cggtcctcag aaagtagatt acgccagcgg cgaaatcagt gcaggtacgg
     3721 cagcgggtac gaaaccgact attgcccttg atactgccgc actgggcggt atgtacgcag
     3781 acagcatcac actgattgcc aatgaaaaag gcgtaggcgt caaaaatgcc ggcacactcg
     3841 aagcggccaa gcaattgatt gtgacttcgt caggccgcat tgaaaacagc ggccgcatcg
     3901 ccaccactgc cgacggcacc gaagcttcac cgacttatct ctccatcgaa accaccgaaa
     3961 aaggagcggc aggcgcattt atctccaatg gcggccggat agagagcaaa ggcttattgg
     4021 ttattgatac cggggaggac atcggtttgc gtaacggagc ggtcgttcaa aacaacggca
     4081 accgccctgc tgctacggta ttaaatgccg gccgtaattt ggttatcgaa agcaaagcaa
     4141 atgtgaacaa tgctaaaggt tcgaccaatc tgtcggccgg cggtcgtact acgatcaatg
     4201 atgctactat tcaagcgggc agttccgtgt acagctccac caaaggcgat actgaattgg
     4261 gtgaaaatac ccgtattatt gctgaaaacg taaccgtatt gtccaacggc agtattggca
     4321 gtgctgctgt aattgaggct aaagacactg cacacatcga agcaggcaaa ccgctttctt
     4381 tggaagcttc aacagttacc tccgatatcc gcttaaacgg aggcagtatc aagggcggca
     4441 agcagcttgc tttactggca gacgataaca ttactgccaa aactaccaat ctgaatactc
     4501 ccggcaatct gtatgttcat acaggtaaag atctgaattt gaatgttgat aaagatttgt
     4561 ctgccgccag catccatttg aaatcggata acgctgccca tattaccggc accagtaaaa
     4621 ccctcactgc ctcaaaagac atgggtgtgg aggcaggctc gctgaatgtt accaatacca
     4681 atctgcgtac caactcgggt aatctgcaca ttcaggcagc caaaggcaat attcagcttc
     4741 gcaataccaa gctgaacgca gccaaggctc tcgaaaccac cgcattgcag ggcaatatcg
     4801 tttcagacgg ccttcatgct gtttctgcag acggtcatgt atccttattg gccaacggta
     4861 atgccgactt taccggtcac aataccctga cagccaaggc cgatgtcaat gcaggatcgg
     4921 ttggtaaagg ccgtctgaaa gcagacaata ccaatatcac ttcatcttca ggagatatta
     4981 cgttggttgc cggcaacggt attcagcttg gtgacggaaa acaacgcaat tcaatcaacg
     5041 gaaaacacat cagcatcaaa aacaacggtg gtaatgccga cttaaaaaac cttaacgtcc
     5101 atgccaaaag cggggcattg aacattcatt ccgaccgggc attgagcata gaaaatacca
     5161 agctggagtc tactcataat acgcatctta atgcacaaca cgagcgggta acgctcaacc
     5221 aagtagatgc ctacgcacac cgtcatctaa gcattaccgg cagccagatt tggcaaaacg
     5281 acaaactgcc ttctgccaac aagctggtgg ctaacggtgt attggcactc aatgcgcgct
     5341 attcccaaat tgccgacaac accacgctga gagcgggtgc aatcaacctt actgccggta
     5401 ccgccctagt caagcgcggc aacatcaatt ggagtaccgt ttcgaccaag actttggaag
     5461 ataatgccga attaaaacca ttggccggac ggctgaatat tgaagcaggt agcggcacat
     5521 taaccatcga acctgccaac cgcatcagtg cgcataccga cctgagcatc aaaacaggcg
     5581 gaaaattgct gttgtctgca aaaggaggaa atgcaggtgc gcctagtgct caagtttcct
     5641 cattggaagc aaaaggcaat atccgtctgg ttacaggaga aacagattta agaggttcta
     5701 aaattacagc cggtaaaaac ttggttgtcg ccaccaccaa aggcaagttg aatatcgaag
     5761 ccgtaaacaa ctcattcagc aattattttc ctacacaaaa agcggctgaa ctcaaccaaa
     5821 aatccaaaga attggaacag cagattgcgc agttgaaaaa aagctcgcct aaaagcaagc
     5881 tgattccaac cctgcaagaa gaacgcgacc gtctcgcttt ctatattcaa gccatcaaca
     5941 aggaagttaa aggtaaaaaa cccaaaggca aagaatacct gcaagccaag ctttctgcac
     6001 aaaatattga cttgatttcc gcacaaggca tcgaaatcag cggttccgat attaccgctt
     6061 ccaaaaaact gaaccttcac gccgcaggcg tattgccaaa ggcagcagat tcagaggcgg
     6121 ctgctattct gattgacggc ataaccgacc aatatgaaat tggcaagccc acctacaaga
     6181 gtcactacga caaagctgct ctgaacaagc cttcacgttt gaccggacgt acgggggtaa
     6241 gtattcatgc agctgcggca ctcgatgatg cacgtattat tatcggtgca tccgaaatca
     6301 aagctccctc aggcagcata gacatcaaag cccatagtga tattgtactg gaggctggac
     6361 aaaacgatgc ctataccttc ttaaaaacca aaggtaaaag cggcaaaatc atcagaaaaa
     6421 ccaagtttac cagcacccgc gaccacctga ttatgccagc ccccgtcgag ctgaccgcca
     6481 acggtatcac gcttcaggca ggcggcaaca tcgaagctaa taccacccgc ttcaatgccc
     6541 ctgcaggtaa agttaccctg gttgcgggtg aagagctgca actgctggca gaagaaggca
     6601 tccacaagca cgagttggat gtccaaaaaa gccgccgctt tatcggcatc aaggtaggta
     6661 agagcaatta cagtaaaaac gaactgaacg aaaccaaatt gcctgtccgc gtcgtcgccc
     6721 aaactgcagc cacccgttca ggctgggata ccgtgctcga aggtaccgaa ttcaaaacca
     6781 cgctggccgg tgccgacatt caggcaggtg taggcgaaaa agcccgtgtc gatgcgaaaa
     6841 ttatcctcaa aggcattgtg aaccgtatcc agtcggaaga aaaattagaa accaactcaa
     6901 ccgtatggca gaaacaggcc ggacgcggca gcactatcga aacgctaaaa ctgcccagct
     6961 tcgaaagccc tactccgccc aaattgtccg cacccggcgg ctatatcgtc gacattccga
     7021 aaggcaatct gaaaaccgaa atcgaaaagc tgtccaaaca gcccgagtat gcctatctga
     7081 aacagctcca agt
//
LOCUS       DAXHKX010000103         7014 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHKX010000103 DAXHKX010000000
VERSION     DAXHKX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.103"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            145..774
                     /locus_tag="ACFMWZ_001484"
     CDS             145..774
                     /locus_tag="ACFMWZ_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC5935431.1"
                     /translation="MIQNVVTSIILYSRTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            920..2581
                     /locus_tag="ACFMWZ_001485"
     CDS             920..2581
                     /locus_tag="ACFMWZ_001485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC5935432.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
     gene            2698..3303
                     /locus_tag="ACFMWZ_001486"
     CDS             2698..3303
                     /locus_tag="ACFMWZ_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC5935433.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3375..3767
                     /locus_tag="ACFMWZ_001487"
     CDS             3375..3767
                     /locus_tag="ACFMWZ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC5935434.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTSPENTSAETAPRPAKKGLKLVK"
     gene            complement(3796..3945)
                     /locus_tag="ACFMWZ_001488"
     CDS             complement(3796..3945)
                     /locus_tag="ACFMWZ_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935435.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(3966..4412)
                     /locus_tag="ACFMWZ_001489"
     CDS             complement(3966..4412)
                     /locus_tag="ACFMWZ_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC5935436.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSVKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(4544..4756)
                     /gene="rpsU"
                     /locus_tag="ACFMWZ_001490"
     CDS             complement(4544..4756)
                     /gene="rpsU"
                     /locus_tag="ACFMWZ_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC5935437.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(5045..6892)
                     /gene="ltgA"
                     /locus_tag="ACFMWZ_001491"
     CDS             complement(5045..6892)
                     /gene="ltgA"
                     /locus_tag="ACFMWZ_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC5935438.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAVATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREIA
                     GKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADIPI
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGTVPAR"
BASE COUNT         1395 a         1884 c         1917 g         1818 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctatattttg acggggttga agttgacatc tacaaagttt aatgtttgaa tacattaaaa
      121 aaatatattt gaatgagatg ttttatgatt caaaatgttg ttacttcaat aatcctgtat
      181 tctaggacag ccgtagactt acttattatc ctaatgttat tttttgccaa aagaaaaagc
      241 agaaaagaca tcattaacat ctatttagga caatttctag gctctgttag cctgatattg
      301 ctaagtttgc tttttgcatt tgtcttagat tatattccta gtaaagagat tttaggtttg
      361 ctcggcttga ttccaatttt cctaggcatc aaagttttgc tgttaggaga ttctgatgga
      421 gaggctattg ccaaagaggg tttgcgcaaa gataataaaa acctgatttt tctagtcgct
      481 atgattactt ttgcaagttg tggtgctgac aatattggtg tctttgttcc atattttact
      541 accttaaatt tagcgaattt gatagtggct ttacttacct ttctagtcat gatttatctc
      601 ttggtttttt ctgcccaaaa attagcacaa gtcccttctg ttggagaaac tttggaaaaa
      661 tatagcagat ggtttgttgc tgttgtttat ttgggattgg gggtatatat cctgattgaa
      721 aacaacagtt ttgatatgct atggactgtg tcgggccagg aaaaaatatt atgaaaaaga
      781 tagtatctgc aaagactgcc atgtgcagtc ttttttgttg ccggtctttt tgtgtctgat
      841 gccgtctgaa gcagtctctg cacgaccttt gtgcgaatat ttgctacact tggcaatccg
      901 tccatttctt ctcacgctta tgctgaccta cactccgccc gatgcccgcc cgcccgccaa
      961 aacccacgaa aagccgtggc tgctgctgtt gatggcgttt gcctggttgt ggcccggcgt
     1021 gttttcccac gatttgtgga atcctgccga acctgccgtc tataccgccg tcgaagcact
     1081 ggcaggcagc cccacccctt tggttgccca tctgttcggt caaaccgatt tcggcatacc
     1141 gcccgtgtat ctttgggttg ccgccgcgtt caaacatttg ctgtcgccgt gggcagccga
     1201 cccgtatgat gccgcacgct ttgccggcgt gtttttcgcc gttgtcggac tgacttcctg
     1261 cggctttgcc ggtttcaact ttttgggcag acaccacggg cgcagcgtcg tcctgattct
     1321 catcggctgt atcgggctga ttccagttgc ccatttcctc aaccccactg ccgccgcctt
     1381 tgccgccgcc ggactggtgc tgcacggtta ttctttggct cgccggcgcg tgattgccgc
     1441 ctcttttctg ctcggtacgg gctggacgct gatgtcgttg gcagcagctt atccggcagc
     1501 atttgccctg atgctgccct tgcccgtact gatgtttttc cgtccgtggc aaagcaggcg
     1561 tttgatgttg acggcagtcg cctcacttgc ctttgccctg ccgctgatga ccgtttaccc
     1621 gctgctcttg gcaaaaacgc agcccgcgct gttcgcgcaa tggctcgacg atcacgtttt
     1681 cggtacgttc ggcggcgtgc ggcacgttca gacggcattc agtttgtttt actatctgaa
     1741 aaacctgctt tggtttgcat tgcccgcgct gccgctggcg gtttggacgg tttgccgcac
     1801 gcgcctgttt tcgaccgact gggggatttt gggcattgtg tggatgcttg ccgttttggt
     1861 gctgcttgcc gtcaatccgc agcgttttca ggataacctc gtctggctgc ttccgccgct
     1921 tgccctgttc ggcgcggcgc aactggacag cctgaggcgc ggcgcggcgg cgtttgtcaa
     1981 ctggttcggc attatggcgt tcggactgtt tgccgtgttc ctgtggacgg gctttttcgc
     2041 catgaattac ggctggcccg ccaagcttgc cgaacgcgcc gcctacttca gcccgtatta
     2101 cgttcccgac atcgatccca ttccgatggc ggttgccgta ctgttcacac ccttgtggct
     2161 gtgggcgatt acccgcaaaa acatacgcgg caggcaggcg gttaccaact gggcggcagg
     2221 cgttaccctg acctgggctt tgctgatgac gctgttcctg ccgtggctgg acgcggcgaa
     2281 aagccacgcg ccggtcgtcc ggagtatgga ggcatcgctt tccccggaat taaaacggga
     2341 gctttcagac ggcatcgagt gtatcgacat aggcggcggc gacctgcaca cgcggattgt
     2401 ttggacgcag tacggcacat tgccgcaccg cgtcggcgat gtacaatgcc gctaccgtat
     2461 cgtccgcctg ccccaaaacg cggatgcgcc gcaaggctgg cagacggttt ggcagggtgc
     2521 gcgcccgcgc aacaaagaca gtaagttcgc actgatacgg aaaatcgggg aaaatatata
     2581 aaaaacaaca gattgagccg aatttctgga ttaagtgccg gaaatcgcgt ataattgcgc
     2641 gattaaacct ttataaatca gccgttttgc aggcatcaca caggagcgac aattattatg
     2701 atgaccctct attccggcat tacctgcccc ttcagccacc gctgccgctt cgttttgtac
     2761 gaaaaaggta tggattttga aatcaaagac gtcgatattt acaacaaacc cgaagacctc
     2821 gccgtcatga acccgtacaa ccaggtcccc gtgctggtcg agcgcgattt ggtgctgcac
     2881 gagtccaaca tcatcaacga atacatcgac gaacgcttcc ctcatccgca gcttatgccc
     2941 ggcgatcccg ttatgcgcgg ccggggccgg ctggtgctgt accgtatgga aaaagaattg
     3001 ttcaaccacg tccaagtgtt agaaaacccc gccgccacca acaaggaaca ggcaaaagcg
     3061 cgcgaagcca tcggcaacgg tctgaccatg cttgcccctt cgttcagcaa aagcaaatac
     3121 atcctcggcg aagatttttc tatgattgat gtcgcccttg ctccgctgct gtggcggctc
     3181 gaccactacg atgtcaaact gggcaaaagt gccgcgccgc tgctcaaata cgccgagcgc
     3241 atcttccagc gcgaagcctt tatcgaagca ctgacacccg ccgaaaaagc catgcgcaaa
     3301 taagtccgaa atgcttgcaa aacccgccgt tttgcaggca ttttcacatt caggcacacc
     3361 acacggaacc catcatgccc acctcaacca aaccctacat cctccgcgca ctttgcgaat
     3421 ggtgcagcga caacagcctc acaccgcaca tccttgtctg ggtcaacgaa cacacgcgcg
     3481 tccccatgca gtacgtccgc gacaacgaaa ttatgctcaa catcggcgcg accgccacgc
     3541 aaaaccttca aatcgacaac gattggatca gcttttccgc ccgcttcggc ggacaggcgc
     3601 acgacatatg gatacctgtc ggacacgtcc tcagcctttt cgcacgggag accggcgaag
     3661 gtatggggtt tgaattggaa gcgtatcgcc ccgacacgtc gcctgaaaac acctctgccg
     3721 aaaccgcgcc ccggcccgcc aaaaaaggct tgaaattggt caaataaatc tatgccgtct
     3781 gaacggaatc gtgtttcaga cggcattttg tccgatgggg cgcaaacgga atccgtttat
     3841 cggcaaaacc cgtttcggtg tatcgaaacc gtgttgcccc tgccgattcg atattgggac
     3901 attgaaaatg ccgtctgaac ctgcgatacg ggcttcagac ggcattttgt ccgatattcg
     3961 ggcaatcagg cggtcagcac ggctttcagg attttgttga cttcgcccat atcggctttg
     4021 ccggcaagtc gggttttcaa tacgcccatc actttgccca tatccgccat acctgccgcg
     4081 ccggtttcgg caacggcggc atcgaccgca gtacggattt cgccggcgga gagcatttgc
     4141 ggcaggtagc ggtgcagtac ctcgatttcg gcgttttctt tgtctgccaa atcctgacgg
     4201 ccggcttcag tgtagatttt cacgctgtct ttgcgctgtt tgaccatttt ggtcaggatg
     4261 gcggtgattt tggcatcgtc ggcttcggtg cgttcgtcca cttcaaactg tttgacggcg
     4321 gcgttgatga ggcggatggt gccgagggaa acttggtctt tggcgcgcat cgcggttttc
     4381 atgtcttcgg taaggcggat tttcaggctc ataatgttct cgctgggatg tcggatggaa
     4441 acggcgggtt tcgatgccgt ctgaaaagca aaacacaccg cagagcaggt cttgcggtgt
     4501 gtcctcatat cacagggctg acctgtaatc tgtacttgaa cgtttagtac attttgggcg
     4561 gcagttgttg gctgcgcagg cgtttttgca ggcgttttac ggctgccgct tttttgcgtt
     4621 tgcgttcggt agtcggtttt tcgtaggctt cgcgggcgcg cagctcggtc agcaggccgg
     4681 ttttttctac ggcgcgtttg aaacggcgca tagcgacttc aaatggttca ttctctttta
     4741 cgcggattgc aggcatttta tttcctttaa taaattcggt tgtttcatct gcccatcata
     4801 tcggtggaaa gggtagggca gacggtgttg aaggtttacc gtatcgctgt gccttgcggc
     4861 ggcgggatac gcgccgtgac ggaaacatca cctcctaacg ggtcggcttg tgccgtgtca
     4921 agctgcttga aaaagcagga aaaacaattt tcggattgtc ttatatttat ggttcgccgt
     4981 caatgccgtt cgggataaaa atgccgtcta aaagaccggg cgggtttcag acggcatcgg
     5041 tacgtcagcg ggcgggaacg gtgcccatac gctgtttgag cgggatgtgc ggcgcgccga
     5101 agagggcggc gtagtaggcg gcattggtca tcactttttt gacatagtct cgcgtttcgg
     5161 aaaacgggat ggtttcggca tataccgcgc cctcaatggg gatatccgcc tgccatcggc
     5221 gcgccctgcc tggaccggcg ttatagcctg cggtggcgag gacttcgttg ttttgcaggc
     5281 ggcgtttggt gtccgccata taccacgtcc ccatacggat attgccgtcg gcggtgtaga
     5341 gctgggcggc atccataccg attttgccag cgatttcgcg cgcggtggca ggcataacct
     5401 gcatcagccc ctgcgcgcct acgcgggatt gcgcgcctat aacgaagcgg ctttcctgac
     5461 gaatcagacc gtaaacccaa gccggatcga cattaacatt ttgcgcgtgg cggattaccg
     5521 tgtctttaaa cggcgaaata tagcgcaagg tgtagttgag tttgcggtcg gtgcgttccg
     5581 cgctgttgac cgccatatcg taaaaaccgt ggtcgaacgc ggtttgcgcg gcggtcagca
     5641 gcttgtcttc gtcaaagccg cgtgtggcaa aacgccattc cgcctgagcc tgacggcgca
     5701 ttttcgcatc gccggcggtt cggctgtttt ggaacagtac cagcgcgcgc ttaatcgcgc
     5761 cgtcttccgc catacggagg acgctgtttt tgccggcatc gggaacattg ttgcgcgtat
     5821 cgattttgcg ccccaactct tcccctgcca gtacggcata aaaattcctg cccgtcgcta
     5881 ccgcctgttt gtagagtttt tccgcctctt gcgtgttgcc cgttgcggcg cggctgcgtg
     5941 ccagccagta gagccaggtc gggctttttt gcagtttttc gggcatatgc gagattacgg
     6001 aagccagctc gtcccaacgt cgggcgcgca aggcggcgcg ggcgtaccac tcgatttggt
     6061 cgtcggtcag ttggcggcgg tcggcaacct tgccgtaata gtccaaggcg gcaggcacat
     6121 tgaggttttg cgactgataa tgccccaata cgccccacgc gaaactgcgt tgttcgaggc
     6181 ttaaaccgct ttccatttcg gacagcaggg cggcggcatt cggcgatttg cgtgcttctt
     6241 tgccgatgac gttcaacagg gcatattcgc gcgaaccttg tgtaccgccg tcaaacgggc
     6301 tgcccaatgc ggcggcaagg ttgcgtgcgt ctgtggtttg gcggccggcc agcagtccgc
     6361 gcacgcgcct ccaggcgtcg ttgccgtcca acaagccgga tgcggctgcc tgttccaaca
     6421 gtttggtgca gcccgaaggc agtttgcccg tatttttgac cagttcggcg gcacgcgtat
     6481 agtcgttgcg gctcgaatcg gcgtagcatt cgacttcttg ggcgcgccct tcaggtttga
     6541 gtttggcata ttcctgtgca aacagcgtcc actgtctgcg tgcgcccaaa gacttcagcc
     6601 actcgttgcg gacattttcc gccatcgcgc tgtcgccggc gttttccaaa taggcggcga
     6661 cggcggcatc gtttttctgt ttcactgcat ccagtgcgga agggtagctg ctgtaatctg
     6721 ccagcgtttt tccttcgggt tcggcagggc gggtgggaac gcttgccgaa agaccggcag
     6781 tttctatatt gtctgccagg gtcttgctgc tgggcgcgtg tgtcgaagaa cacgcggcaa
     6841 gcaccagggc cgccagcagc ggcagggaat gcttcataga gggtaggtac atcggatttc
     6901 cttaagaatc ggaaccctga acggtcaggg ttggaaaaga caaaatgccg tctgaacagg
     6961 cgtttgcccg aattatatgc cgaaactgca ccgcctttgg aatgtttccg acat
//
LOCUS       DAXHKX010000104         7000 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHKX010000104 DAXHKX010000000
VERSION     DAXHKX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.104"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(75..1496)
                     /locus_tag="ACFMWZ_001492"
     CDS             complement(75..1496)
                     /locus_tag="ACFMWZ_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC5935439.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1511..1891)
                     /locus_tag="ACFMWZ_001493"
     CDS             complement(1511..1891)
                     /locus_tag="ACFMWZ_001493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HFC5935440.1"
                     /translation="MSPYGCGAGPNLECRTERDKGKQTGGKPVSLQIYVKINSFFVFD
                     RLFIKDIYLFVLNKIIYQKEHIFPSTAIRLPAAFRFGKLVYPDYLLYNQSENFFQKQT
                     GSTVRLCPGGTVPVSLPRAAQGSA"
     gene            complement(1854..2213)
                     /locus_tag="ACFMWZ_001494"
     CDS             complement(1854..2213)
                     /locus_tag="ACFMWZ_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC5935441.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2210..2815)
                     /locus_tag="ACFMWZ_001495"
     CDS             complement(2210..2815)
                     /locus_tag="ACFMWZ_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC5935442.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIIQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWVKLGITSCPYPHNKKNISK"
     gene            complement(2862..3344)
                     /locus_tag="ACFMWZ_001496"
     CDS             complement(2862..3344)
                     /locus_tag="ACFMWZ_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC5935443.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3548..3645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3854..4954
                     /gene="gcvT"
                     /locus_tag="ACFMWZ_001497"
     CDS             3854..4954
                     /gene="gcvT"
                     /locus_tag="ACFMWZ_001497"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC5935444.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5116..5502
                     /gene="gcvH"
                     /locus_tag="ACFMWZ_001498"
     CDS             5116..5502
                     /gene="gcvH"
                     /locus_tag="ACFMWZ_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC5935445.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            5649..6896
                     /gene="hemA"
                     /locus_tag="ACFMWZ_001499"
     CDS             5649..6896
                     /gene="hemA"
                     /locus_tag="ACFMWZ_001499"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC5935446.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1788 a         1673 c         1953 g         1586 t
ORIGIN      
        1 tgtcggaagg ggggatattg atttgattat gccggaattt aaaatgccgt ctgaatgttc
       61 agacggcata gcgtttacag cagtttgaaa acgaaaaaga taagggtatg tacgatgaag
      121 acgggtgtca ggaaggcgac cgaccacatc atatagccga agaaagtcgg catcggtacg
      181 ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg cgttgccgat gtaggtcagt
      241 gcgcccatga ataccgaacc catagaaacc gccagcagcg aatgaaacag ggtacccgtc
      301 atcaaggctt gggcatcgcc gcccgccata ttgaaaaaaa cgagataagt gggcgcgtta
      361 tccaagaatg ccgacaatat gccgctcatc caaaaataca tcacattaat cggatgacct
      421 gccgtatcgt gaaccagcga taccaccccg cccagcgcgc ctgcctcgcc tgctttcaga
      481 atgctcagga cgggaaagat ggtgatgaag atgccgagga agagtttgcc cacttcggcg
      541 atgggttcaa agttgaattc gttgcctgcg cggacttgtt tgggcgtgat tgccatagat
      601 acggcggtca atgcaatcag gatgacatcg cggacgaggt tttgcagggc gtaacggctg
      661 ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc cggacattag aaccgcgccg
      721 accacgcccg aaagcaggag gaagttccat ttgccgaaga tggcgatttt ttcgggtttt
      781 tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt tgaagaaacg gttgtcgatg
      841 aaatagaagg cggtcaacag gacagcggtg ctgatcagga cgggggcgaa catatgtttg
      901 accgtccaca tgaaatctac gcctttgagg aagccgagga agagtggggg gtcgcccaaa
      961 ggggtcagac cgccgccgat gtttgcaacc aggaaaatga agaagatgac gatgtgcacg
     1021 cggcgggtac ggttttggtt ggctttcagc agcggacgaa tcatcagcat tgctgcgccg
     1081 gtcgttccca tgatagaggc aagtgccgta ccgacggcaa gcagggcggt gttgagcttg
     1141 ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg aaatggtgta cagggcaagc
     1201 agcagcagga tgaaagggat gtattcttca acgagtgcgt gtgcgacggt atggataccg
     1261 gcggacgcgc caaaaaccaa actgaacggg atgaggaaga gcaatgtcca aaaggcggta
     1321 attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca agggacccaa tgcgatagac
     1381 agcaaaatca gggcaaaggg caggctccac agcaggttta ggtttgcgcc gtccaaatct
     1441 gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca ggggtaggtg gcgcatcgtg
     1501 tttcctcgat tcaagcactg ccttgcgcgg cgcgtgggag tgatacaggc accgtgccgc
     1561 ccggacatag gcggactgtg ctgcctgttt gtttttgaaa aaagttttcc gattgattgt
     1621 aaagtaaata atcaggataa accagtttcc caaaccggaa ggcggcggga aggcggattg
     1681 ctgtgcttgg gaatatatgt tctttttgat aaataatttt atttaaaaca aataaatata
     1741 tatctttaat aaataatcta tcgaaaacga aaaatgaatt tattttaaca tatatttgca
     1801 atgaaacagg tttgcccccc gtttgtttgc ccttatccct ttcagtacgg cattcaagat
     1861 tcgggcctgc gccacatcca tatggcgaca agggaacaaa aaaccgatga aaccgccccg
     1921 acccaccagc gttggggaaa ctgccaaaac attatcaggc aggatgcggt catcatactg
     1981 atggcgaata ttttggcttt gcgcggcact gcgccgtttt gttcccagtt atgaaccatc
     2041 ggaccgaaat agcggtgtcg gtgcagccag cggtgaaagc gcggggatgc ctttgcccag
     2101 caggcggcgg agagcagtac gaacggcgtg gtcggcaaca gcggcaaaaa aatgccgatt
     2161 atccccaaca gcagggagat gccgccgcag gcaattaaaa gataacgtat cattttgaaa
     2221 tatttttctt attgtgcgga taagggcagg atgtgatacc gagttttacc cagccctcat
     2281 gtcccatttt ttccagcagg gcgatattgc gttcgaatat tgccgaagcg tcgggaaagg
     2341 cttgtgcggc tttggcaatg ctgtcttcgc gtatcagatg cagtgtcgga tagggagaac
     2401 ggttggtgta gttgccgatg tcgtccgaat ccgtgccttc aaattggaaa tcgggatgaa
     2461 acggggcgat ttggatgatg ccttccaagc cgttttcgac aacggcggca tcggcaatat
     2521 cgagcatatc gttgaatacg tcgaaatcgg ggaatagggt cgggtgaacc agcagggtgg
     2581 tttccagttc ggtggcgggt gtattgccca gtcgctgcag ttcttcgtcc aagtcttcca
     2641 aaaaactgtc aaggtgtttg gcttcgctga ttgcgatgcg gacaaggttt ttaacgtggg
     2701 gggctttggc aaagggacac aggttcagac cgatgacggc tttttccaac cattgtccgg
     2761 tgtgttcggc aacagcatct ttattttcgg aagtattgat attcattatt gtcatgtaaa
     2821 tgtgtttgca gattgcacgt gcgggaaaat cgggaagggc actattcctt cagcaggtgg
     2881 ttgagcggca gggaggtggt gtgtttgatt tcttttaaaa caaagctcga ttgcgcatct
     2941 tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat gggaaaacgc gttcatatcg
     3001 gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca gggcgaagca gctcaagact
     3061 tcaggccatt ttcgaaccga tgcggcaaag tcttcccgcg cgtcttttgc tttgcggatg
     3121 gaaacgcgga taaatgcctg aagccccagg ttgacggatt ccggagacag cagcgcggca
     3181 tattggcgga cgatgccggc atcttccaac tgcttcagac ggcgcaggca cggagaaggc
     3241 gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc tgccgttttc ctggagaacc
     3301 tgtaagattt taatatcggt tttgtctaaa gtgagttggg gcatatttgc gttccgtttt
     3361 aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc gcacagatgg agaccatatt
     3421 aacatataaa aagttatacc gtcatccggg acaaattttg ttttcggaaa atcatgtgaa
     3481 aacagaggcg gtcggtttgc atctctttta agacggcttg cccaaaccgc cgattcaaga
     3541 cataatcggg aaatgtgcag gagagtgtta cacccaacta caatgtaacc accgaaggcg
     3601 cagacaccct taaatcgctc aggtatcagg gactgcacat tgaaacaaac aatctggaga
     3661 gcggcgttgg aataacgtcc accgaagggg agaaggccgt ctgaaccacc attcagacaa
     3721 ccgcgcaaag cagtgagcag actggtttgc catcatgcgg atacggccga aaatctcagg
     3781 ttcaaggaca gatagggtca tccgcgcatc ttgtgcggac ggcatctgaa caataaatcc
     3841 agagaagctg agaatgactg ccctgaaaac caccccattt catcaagccc atcaagatgc
     3901 aggcgcgaag ccggtagatt ttgccggctg ggagctgccc atccattatg gttcgcaaat
     3961 cgccgaacac gaagccgtgc gcaccgacgc cggtatgttt gacgtatccc atatgcttgt
     4021 taccgacgta gcaggcgcaa atgccaaagc ctttttccgc aaattgattg ccaacgatgt
     4081 cgccaagctc gcttttgtcg gcaaagccct ttattccgct ttgctcaacg acaacggcgg
     4141 cgtgattgac gacttaatcg tttaccgcac caacgaagcc gaaacccaat accgcatcgt
     4201 gtccaacgga gcgactcgcg aaaaagacac ggcgcaattc cacaaagtcg gacaagagtt
     4261 cggcgttgcc tttaatccgc gctacgacct cggcatgctc gccgtacaag gccctaaagc
     4321 catcgaaaaa ctcctgaccg tcaaacctga atgggcggat gtgattcacg gcctcaaacc
     4381 gttccaaggt gcagacttgg gcaacgattg gtttgtcgcc cgcaccggct ataccggcga
     4441 agacggcgtc gaagtgattc tgccgggcac tgaagccgtc gcattcttca aagccctgca
     4501 agcagcaggc gtacaacctt gcggtctcgg cgcgcgcgac accctgcgca tggaagccgg
     4561 catgaacctc tacggcaacg atatggacga cgataccagc ccgctcgaag caggcatggg
     4621 ttggaccgtt gatttgaaag acgaaagccg cgattttgtc ggcaaagctg ccttgttggc
     4681 attgaaagaa aaaggcgttg ccgtcaaaca ggtcggtttg ctgctcggca aaggcggcat
     4741 cctgcgcgcg catatggaag tgttgaccga caaaggcaaa ggcgaaacca ccagcggcgt
     4801 attctcgccc agcctgaaac aatccatcgc catcgcccgc gtaccgaaag actttgacgg
     4861 cgataccgct aaagtgctga tgcgtggcaa ggaagtggac gtgcgcgtat tgaagttgcc
     4921 gttcgtgcgt aacggtcaaa aacagtttga ttaatatcgg ttcagacggc attttcattt
     4981 catatgccgt ctgaaagcag gttttaattg ttgtccgata cggacgtttg tagaaagcat
     5041 tgaacaaggc atctgtggat attgattcat gcagatgccg tctgaaaata acccctatca
     5101 atggagtatc aaaccatgag caacaacatc ccggccgaac tgaaatacgt tgccagccat
     5161 gaatggctgc gccttgaaga agacggtacg attaccgtcg gcattaccca ccacgcgcaa
     5221 gagctgttgg gcgacatcgt gttcgtcgag ctgcccgaag tcggcgcgaa ccttgccgct
     5281 gaagagcaag ccggtgtggt tgagtctgta aaagccgcgt ccgacgtgta cgcaccgatt
     5341 gcaggcgaag tcgttgccgt caacgaagat ttgccaagtg ctccggaaac tgccaacagc
     5401 gattcttacg gcgcaggctg gttcttcaaa atcaaacctg tcaatcctgc cgattacgac
     5461 ggtctgctga ctgccgaaca atacgcgggc gaagtggact aaatctctat cctgataatg
     5521 ggatgactga tttgagaaac tgcactttca gacggcaagg atgcgtttgg aagtgcagtt
     5581 tttgtctgtg tcttcaaagt tcagttaggc tataataacg gtctatattc atctttaccg
     5641 attttttcat gcaacttacc gctgtcggac tcaatcatca aaccgcacct ttaagcatac
     5701 gggaaaagct ggcgtttgcc gccgccgccc tgcctaaagc cgtccgcaat cttgcccgaa
     5761 gcaatgcggc aacggaggcg gtaatccttt ctacctgcaa ccgcaccgag ctttactgcg
     5821 tcggtgattc ggaagaaatc atccgatggc ttgccgatta ccacagtttg ccgattgaag
     5881 aaatccgtcc gtatctgtac gcgctggata tgcaggaaac cgtgcgccat gccttccgcg
     5941 ttgcctgcgg cttggattcg atggtgttgg gcgagccgca gattttagga cagattaagg
     6001 atgcggtcag ggttgctcaa gagcaggaaa gtatgggtaa gaaactcaat gccctgttcc
     6061 aaaaaacctt ttctgttgct aaagaggtcc gtaccgatac tgccgtcggc gaaaactcgg
     6121 tttccatggc ttccgcttcc gtcaaattgg cggaacagat ttttcccgac atcggcgatt
     6181 tgaatgtctt gtttatcggc gcaggcgaaa tgattgagct ggttgccact tattttgccg
     6241 ccaaaagtcc ccggctgatg acggttgcca accggacgct ggcgcgtgca caggagttgt
     6301 gcgacaagct cggtgtcaac gccgaaccgt gcctgctgtc cgatctgcct gccattctgc
     6361 acgattacga cgtagtggtt tcttcaacgg caagccagtt gcccattgtc ggcaaaggca
     6421 tggtggagcg tgcattgaaa caaaggcaga gtatgccgtt gttcatgctt gatttggcag
     6481 tgccgcgtga cattgaagcg gaagtcggcg atttgaacga tgcctatctt tatacggtgg
     6541 acgatatggt caacatcgtc caaagcggca aggaggcaag gcagaaggcc gccgccgccg
     6601 ccgaaacgct ggtatccgag aaggttgccg aatttgtcag gcagcagcag ggcaggcaga
     6661 gcgtcccctt gattaaagcc ttgcgggacg agggcgagaa agcgcgcaag caggtgttgg
     6721 aaaatgcgat gaaacagctt gccaaaggcg caacggcaga agaggttttg gaacggctgt
     6781 ccgtccaact gaccaacaag ctgctgcatt cgccgaccca aactttgaat aaggcggggg
     6841 aagaagataa agatttggtt catgccgtcg cgcagattta tcatttggac aaataacggt
     6901 gcgccggaaa aggatgccgt ctgaaggggt ttcagacggc atatttttgt gctgtctgac
     6961 aacatcgtga aatcccacat tatatcgatg taatcacaaa
//
LOCUS       DAXHKX010000105         6842 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHKX010000105 DAXHKX010000000
VERSION     DAXHKX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.105"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="ACFMWZ_001500"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="ACFMWZ_001500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC5935447.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1512
                     /locus_tag="ACFMWZ_001501"
     CDS             1102..1512
                     /locus_tag="ACFMWZ_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC5935448.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            1533..3596
                     /gene="glyS"
                     /locus_tag="ACFMWZ_001502"
     CDS             1533..3596
                     /gene="glyS"
                     /locus_tag="ACFMWZ_001502"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC5935449.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLVVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAAQMREQGKVVA
                     SFAERKAAIQTALEGQARRLNASVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3613..4659
                     /locus_tag="ACFMWZ_001503"
     CDS             3613..4659
                     /locus_tag="ACFMWZ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC5935450.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIARRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4701..5528
                     /locus_tag="ACFMWZ_001504"
     CDS             4701..5528
                     /locus_tag="ACFMWZ_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC5935451.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQV
                     MAELVPGLSAHPYLSGVEKACFMSHVVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISK
                     KAIRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5540..5635
                     /locus_tag="ACFMWZ_001505"
                     /pseudo
     CDS             5540..5635
                     /locus_tag="ACFMWZ_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            5677..6519
                     /locus_tag="ACFMWZ_001506"
     CDS             5677..6519
                     /locus_tag="ACFMWZ_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC5935452.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1757 a         1824 c         1794 g         1467 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacatt ctgggcagac aaaggctgca
      181 ccgttatcca acccttcgac atggaagtcg gtgcaggtac ttcccacccc gccacctgcc
      241 tgcgcgcact cgggcccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggtcg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaagcc cgctcccgcc aatatccaag acctctatct cgactccctg cgcgaattgg
      421 gcatcgaccc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggtgcatg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcattgact gcacgcctgt actcggcgaa attacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgcc ggacggcaac accgtaagct acggcgacgt gtaccaccaa aacgaagtcg
      721 aacaatccac ctacaacttc gaatacagcg atgccgattg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccctgcctg
      841 cctacgagct ggtcctcaaa gcggggcata cgttcaacct cttggacgcg cgcggcgcga
      901 tttccgtaac tgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaatgctg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccggaagcc
     1081 attatatttt ttaggaaaat tatgcctgtc tatttccgca ccccgagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca agcattgatt
     1201 ttcggcgttc gaggcagttt gcaccaagct gcggacgatg ctgaagcgtt cgaccgcttt
     1261 atggacgagc tgattgcgta tcttgaggca aatggcttgg aaatggcggg cgggggcgat
     1321 tgcggcgatt tttcgtgcct gtttcaaaaa tccggtgccc gaagcttggg cgaaaccgag
     1381 aaaaaagcca ttatctcttg gctggcaggc cgcgaagatg tcgccacttt gtacgccacc
     1441 ccgctggttg atggttttta cggggatgac ggctgggaaa attacagcga aacctataaa
     1501 caggcagatt aaccaaattc aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac
     1561 tccttaccga agaactcccg cccaaagctc tgaataatct gggcaaccat tttgccgccg
     1621 ccgttgccga aggcttggaa aaagcgcaac tggttgacgg tgcagccgaa tttacggctt
     1681 atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt taaagccgtt caggccgacc
     1741 aaaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccat gaaagacggt gcgccgacca
     1801 aggctttgga aggtttcgca cgcggtgcgg gcgcgaaaat cgaagacttg accatcgtcc
     1861 acgacggcaa gcaggacgtg tacgcctacg aatacgtcca aaccggcaaa ccgttgggcg
     1921 agcttttgga agacattatc aatgccgcag tgaaaaaact gccgattccg aaagtcatgc
     1981 gttggggcag cagcacgttt acctttgtgc gccccgttca cgggctagtt gtactgcacg
     2041 gcggcgacat cgtaaacgtc agcgttttgg gtctgcaaag cagcaatcaa accttgggac
     2101 accgcttctt gtccaacggc gaaatcacca ttgaaaacgc cgacagctac gccgcacaaa
     2161 tgcgcgagca aggcaaagtc gtcgcttcgt ttgccgagcg caaagctgcg attcagacgg
     2221 cattggaagg gcaggcacgc cgtctgaacg cctccgttgc cgccgatgaa gcattgttgg
     2281 acgaagtaac cgcgctggtc gaatggcctg tggtattgga agccggtttt gaggaacatt
     2341 tcctcgccgt gccgcaggaa tgcttgattt taacgatgca gcaaaaccaa aaatactttc
     2401 cgctgctcga ccaaaacggc aagctgatga accgcttcct gctggtttcc aacctgcaaa
     2461 ccgaagaccc gtcgcacatc atccgaggca acgaacgcgt tttgcgcgcg cgcctgtctg
     2521 atgccgagtt cttctacaaa caagaccaaa aagcgacttt ggaaagccgc ctgccgaaat
     2581 tgtccagcgt ggtgtaccac aacaaaatcg gctcgcaagc cgaacgcatc gaacgcctgc
     2641 aaagcattgc cgcccacatc gccaaagcat tgggcgcgga tgccgccgct gccgaacgcg
     2701 ccgcgcgtct ggcaaaagcc gacttggtaa ccgaaatggt cggcgagttc cctgaattgc
     2761 aaggcaccat gggcaaatac tatgcccgtt tggacggcga aaccgaagaa atcgccgaag
     2821 ccatcgagca gcattatcag ccgcgttttg ccggcgacaa gctgcccgaa agcaaaattg
     2881 ccgccgccgt ggcactggcg gacaagctgg aaaccctggt cggcatttgg ggcatcggtc
     2941 tgattccgac cggcgataaa gacccctacg ccctgcgccg cgccgccttg ggtattttgc
     3001 ggatgctgat gcagtacggt ttggacgtga acgaactgat tcagacggca ttcgacagct
     3061 tccccaaagg tttgctcaac gaaaaaacgc cgtctgaaac cgccgacttt atgcaggcgc
     3121 gccttgccgt gttgctgcaa aacgattatc cgcaagacat cgtcgccgcc gtactcgcca
     3181 aacagccgcg ccgtttggac gatttgaccg ccaaactgca agccgttgcc gcgttcaaac
     3241 agctgcccga agccgccgcg ctcgccgccg ccaacaaacg cgtgcaaaac ctgctgaaaa
     3301 aagccgatgc cgcgttgggt gcggttaacg aaagcctgtt gcaacaggac gaagaaaaag
     3361 ccctctttgc cgccgcgcaa ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca
     3421 atttccaaac cgccttgtcc gaactggctt ccgtcaaacc gcaagtcgat gcattctttg
     3481 acggcgtgat ggtaatggcg gaagatgccg ccgtaaaaca aaaccgcctg aacctgctga
     3541 accgcttggc agagcaaatg aacgcggtag ccgacatcgc gcttttgggc gagtaaccgt
     3601 tgtacagtcc aaatgccgtc tgaagccttc agacggcatc gtgcctatcg ggagaataaa
     3661 ttgcagcctt tagtcagcgt attgatttgc gcctacaacg tagaaaaata tttcgcccaa
     3721 tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg atattttgat tgttgatgac
     3781 ggctcgacgg acggcacact tgccattgcc cggcgtttcc aagaacagga cggccgcatc
     3841 cgcatcctcg cgcagccgcg caattccggc ctgattccct ctttaaacat cgggctggac
     3901 gaattggcaa agtcaggaat gggggaatat attgcacgca ccgatgccga cgatattgcc
     3961 gcccccgact ggattgagaa aatcgtgggc gagatggaaa aagaccgcag catcatcgcg
     4021 atgggcgcgt ggctggaagt tttgtcggaa gaaaaggacg gcaaccggct ggcgcggcat
     4081 cacaggcacg gcaaaatttg gaaaaagccg acccgacacg aagatattgc cgactttttc
     4141 cctttcggca accccataca caacaacacg atgattatga ggcgcagcgt cattgacggc
     4201 ggtttgcgtt acaacaccga gcgggattgg gcggaagatt accaattttg gtacgatgtc
     4261 agcaaattgg gcaggctggc ttattatccc gaagccttgg tcaaataccg ccttcacgcc
     4321 aatcaggttt catccaaata cagcgtccgc caacacgaaa tcgcgcaagg catccaaaaa
     4381 accgccagaa acgatttttt gcagtctatg ggttttaaaa cccggttcga cagccttgaa
     4441 taccgccaaa taaaagcagt agcgtatgaa ttgctggaga aacatttgcc ggaagaagat
     4501 tttgaacgcg cccgccggtt tttgtaccaa tgcttcaaac ggacggacac gctgcccgcc
     4561 ggcgcgtggc tggattttgc ggcagacggc aggatgcggc ggctgtttac cttgaggcaa
     4621 tacttcggca ttttgcaccg attgctgaaa aaccgttgaa aaacgccgct ttatccaaca
     4681 gacaaaaaac aggataaatt atgcaaaacc acgttatcag cttagcttcc gccgcagaac
     4741 gcagggcgca cattaccgat accttcggcg tgcgcggcat cccgtttcag tttttcgacg
     4801 cactgatgcc gtctgaaagg ctggaacagg taatggcgga actcgtaccc ggtttgtcgg
     4861 cgcaccccta tttgagcgga gtggaaaaag cctgctttat gagccacgtc gtattgtgga
     4921 agcaggcatt ggacgaaggt ctgccgtata tcaccgtatt tgaggacgac gttttactcg
     4981 gcgaaggcgc ggagaaattc cttgccgaag acgcttggct gcaagaacgc tttgaccccg
     5041 attccgcctt tatcgtccgc ttggaaacga tgtttatgca cgtcctgacc tcgccctccg
     5101 gcgtggcgga ctacggcggg cgtgcctttc cgctgttgga aagcgaacac tgggggacgg
     5161 cgggctatat catttccaaa aaagcgatac ggtttttcct ggacaggttt gccgccctgc
     5221 cgcccgaagg gctgcacccc gtcgatctga tgatgttcag cgattttttc gacagggaag
     5281 gagtgccggt ttgccagctc aatcccgcct tgtgcgccca agagctgcat tatgccaagt
     5341 ttcacgacca aaacagcgca ttgggcagcc tgatcgaaca cgaccgcctc ctgaaccgca
     5401 aacagcaatg gcgcgattcc cccgccaaca cattcaaacg ccgcctgatc cgcgccttga
     5461 ccaaaatcag cagggaaagg gaaaaacgcc ggcaaaggcg cgaacagttc attgtgcctt
     5521 tccaataaaa ggaggagaga tggacatcgt atttgcagca gacggcagga tgcggcggct
     5581 gtttaccttg aggcaatact tcggcatttt gcgccgattg ctgaaaaacc gttgaaaaac
     5641 gccgctttat ccaacagaca aaaaacagga taaattatgc aaaaccatgt tatcagctta
     5701 gcttccgccg cagaacgcag ggcgcacatt gccgcaacct tcggcgcgcg cggcatcccg
     5761 tttcagtttt tcgacgcact gatgccgtct gaagaactga atcggatgat ggcggaattg
     5821 gtgccgggat tggcaaaaca gcacctgttg agcgaagtgg aaaaagcctg ctttatgagc
     5881 cacgccgtat tgtggaaaca ggcattggat gaaggtctgc cctatgttgc cgtatttgag
     5941 gatgatgtcc tgcttggcaa agacgcagaa aagttccttg ccgaagatac ttggttggaa
     6001 gagcgttttg ataaggattc tgcctttatc gttcgtttgg aaacgatgtt tgcgaaagtt
     6061 attgtcagac cggataaagt cctgaattat gagaaccggt catttccttt gctggagagc
     6121 gaacattggg ggacggctgg ctatatcatt tcgcgtgagg cgatgcggtt tttcttggaa
     6181 aggtttgccg ttttgccggc agagtggatt aaagcggtag attggatgat gtttacttat
     6241 ttctttgata aggaggggat gcctgtttat caggttaatc ccgccttatg tacccaagaa
     6301 ttgcattatg ccaagttcct cagtaaaaac agtatgttgg gtagcgattt ggaaaaagat
     6361 agggaacaag aaagaagaca ccgccgttcg ttgaaggtga tgtttgactt gaagcgtgct
     6421 ttgggtaaat tcggtaggga aaagaagaaa agaatggagc gtcaaaggca ggcggagctt
     6481 gagaaagctt acggcaggcg ggtcatatcg ttcaaatagt ttgtgtaaaa tatagcggat
     6541 taaaattaga aatggataca ctgtcattcc cgcgcaggcg ggaatctagt ccgttgagtt
     6601 tcagtcattt ccgataaatc accgcaacgt taaatttcta gattcccgcc tgcgcgggaa
     6661 tggcggtggt gggatttctg tttttccgac aaattcctaa aacttaaagt tccgttattc
     6721 ccgcgaaaac agaaaaccaa aaatagaaac ctgaaaacag aaacctgaaa acagaaacct
     6781 aaaattcgtc attcccgcgc aggcgggaat ccagtccgtt gagtttcagt catttccgat
     6841 aa
//
LOCUS       DAXHKX010000106         6740 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHKX010000106 DAXHKX010000000
VERSION     DAXHKX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.106"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="ACFMWZ_001507"
     CDS             complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="ACFMWZ_001507"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC5935453.1"
                     /translation="MSDNVPTIAAVATASGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1556..1711
                     /locus_tag="ACFMWZ_001508"
     CDS             1556..1711
                     /locus_tag="ACFMWZ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935454.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPTKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1708..2139
                     /locus_tag="ACFMWZ_001509"
     CDS             1708..2139
                     /locus_tag="ACFMWZ_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC5935455.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2273..6574
                     /gene="app"
                     /locus_tag="ACFMWZ_001510"
     CDS             2273..6574
                     /gene="app"
                     /locus_tag="ACFMWZ_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC5935456.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYQKDTKHYYHEDYHN
                     PRLNKFVTDAAPVEMTINMNGNTYGNITDYPDRVRIGSGRQYWHKKNGNPQQIFGSYS
                     YRIAGNTHNQVGGSDGHVSLNGDVKKPNNYGPLPTGVSFGDSGSPMFIYDAKKQKWLI
                     NGVLREGNPYQGTHNGYPIVRKAYLTKIQDGDTGASIYDMVGGASYLWEAKELGKSTI
                     SQENNPKFSQMSDIANPSLPKQNKYTPSLDHRKHITFGEYVEGGVGKLTLKSNIDQGA
                     GGLYFEGDFIVSPTQNETWKGAGISISDESTIIWKVNGVANDRLSKIGKGTLHVQAKG
                     ENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGRGTVQLNADNQFNPDKLYFGFR
                     GGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVTITGNKDIATTGNNNSLDSKKE
                     IAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNGNITQTNGKLFFSGRPT
                     PHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQGGQAVISRNVAKVEGDW
                     HLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKVIASLTKTDISGNVSLA
                     DHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGDLSLVGNAQATFNQATLNGNTSA
                     SGNASFNLSNNAVQNGSLTLSDNAKANVSHSALNGNVSLADKAVFHFESSRFTGQISG
                     SKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAAGAQTGSVSDTPRRRSR
                     RSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRSDKLKLAESSEGTYTLA
                     VNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGAWRYQLIRKDGEFRLHN
                     PVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAGRDAVEKTESVAEPARQAGGENVG
                     IMQAEEEKKRVQVDKDSALAKQREAETQPATTAFPRARRARRDLPQPQPQPQPQPQPQ
                     RDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFR
                     AYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNSARLAHGAVFGQYGIGR
                     FDIGISTGAGFSSGSLSDGIGSKIRRRVLHYGIQARYRAGFGGFGIEPHIGATRYFVQ
                     KADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPYLSLSYTDAASGKVRTR
                     VNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1830 a         1908 c         1640 g         1362 t
ORIGIN      
        1 aaccaaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc ggacaccgcc
       61 acgatgcttt cgatccgttt attttccgat acaaaacctc gaaaaaatca cgccgagcag
      121 gtcgtccgcc gtaaactcgc ccgtgatttc gccgcatgcg acctgcgcca agcgcaagtg
      181 ttcggcaaac agctcgattt gatggttgcc gcacaatgcc gccagcgaca attcttcctg
      241 cgctgctttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt cgccctcgcc
      301 ctgccatccg gcctcgcgca acaacgtccg tttcagcgcg tccaagccgt cgccggtttt
      361 cgccgacagc gcgatgacgg tttctgcgcc cgtaccgaac ccgcctgccg cgtgtgcgtg
      421 caaatcggat ttgctgtgga tttcgatgcg tttcaactcc ggcggcaacg cgtccaaaat
      481 cgcccgtgtc ttttcattca aaccctcgcg cggatcgacc aacaccagcg cgacatcggc
      541 ttcggatacg gctttgcggc tgcgttcgat gccgatacgc tcgaccacgt cgtccgtctc
      601 gcgcaaacct gccgtatcga caatatgcac cggcacgccg tcaatcagga tacgttccct
      661 gaccgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt cgccagccaa
      721 cgcgttcagc aggctggact tgcccacatt cggcgcgccg accaatacga cattcagacc
      781 ttcgcgcaaa atcgcgccct gctgcgcgtt ggcaagcaca tcatccacgg cgcggcgcaa
      841 gccgtccagt ttgccgcgtg cgtctgccgc ttcgagaaaa tcaatgtctt cctcgggaaa
      901 atccagcgtc gcttcgacca gcatccgtaa ggtaatcaag tcttcgacca gaccgtgtat
      961 ccgccgcgaa aaatcgccct tgagcgagcg caacgccaga cgcgccgccg aacggctgga
     1021 tgcgtcaatc aaatccgcca cgccttccgc ctgtgccaag tccagtttgt cgtttaaaaa
     1081 cgcacgtttg gtaaactcgc ccggttcggc aaggcgcgcg cccaactcca aacagcggtt
     1141 cagcagcata tccatcacca ccggcccgcc gtgtccctga agctcgatga catcttcacc
     1201 cgtaaaactt gccggtgcgg caaaaaacag caaaagcccg ctgtcgattg cctgaccgtc
     1261 cgcgtccgta aaatcagcat aggttgccac gcgcggcttg ggcgttttac cgcacaaagc
     1321 ctctgccatc ggcagcaggt ttttccccga tatgcgtatc acgcccacgc cgccgcgccc
     1381 tgatgcggta gcgactgccg caatcgttgg aacgttatcc gacataaaac ccccgaaaat
     1441 tcaaaacagc cgcgattata gcaaatgccg tctgaagtcc gacggtttgg ctttcagacg
     1501 gcataaaacc gcaaaaacgc ttgataaatc cgtccgcctg acctaatata accatatgga
     1561 aaaacgaaac acatacgccc tcctgctcgg tataggctcg ctgttgggcc tgttccatcc
     1621 cacaaaaacc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     1681 tcaacgcgcc atagagaaag cgcgtaaatg accgaaaacg cacaggacaa ggcgcggcag
     1741 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     1801 gtgcaaataa tgatagcccg gcgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     1861 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     1921 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     1981 ctgtacggct tcatcagcgt catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2041 ggctaccccg caaccgccgc ctcccttgcc ggcggcacag tggttgcctt ggcgggtgct
     2101 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2161 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgcgcgcc gcccggaagc
     2221 ggaagccgcg ccctcccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     2281 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggtc gcatccgctt
     2341 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cctgggcggg
     2401 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     2461 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     2521 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     2581 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggctata acaacgttga
     2641 ttttggtgcg gagggaagca atcccgatca gcaccgcttt tcttaccaaa ttgtgaaaag
     2701 aaataattat caaaaggata caaagcatta ctatcacgag gattatcata atcctagatt
     2761 aaataaattt gttactgatg ccgctccagt agaaatgacc attaatatga atggtaatac
     2821 ttatggaaat ataactgact atcccgatcg tgtgcgtatt ggatcagggc gacaatattg
     2881 gcataaaaaa aatggtaacc ctcaacaaat ctttggtagt tatagttacc gaattgctgg
     2941 taatacccat aatcaagtgg gaggaagtga tggacacgtt agtttaaatg gggacgtaaa
     3001 aaaaccaaac aactatggcc ctcttcctac gggtgtttca tttggagata gtggttctcc
     3061 aatgtttatt tatgatgcaa aaaaacaaaa atggctaatc aatggtgtat taagagaagg
     3121 taatccatac caaggaacac acaatggcta tccaattgta cgtaaagcct atttaacaaa
     3181 aatccaagat ggagatactg gagcgagtat ttatgatatg gtgggtgggg cttcttatct
     3241 gtgggaagct aaagaattag gaaaaagcac tattagccaa gaaaataatc caaaattttc
     3301 tcaaatgtca gatattgcta atcctagctt gcctaagcaa aacaaatata ccccatcgtt
     3361 agatcacaga aaacacatta cttttggaga atatgttgaa ggcggagttg gtaaacttac
     3421 tttgaaatca aatattgatc aaggtgcagg cggtttgtat tttgagggag attttatagt
     3481 ttcgcctacc caaaatgaaa catggaaagg ggcgggtata tctatcagtg atgaaagtac
     3541 cataatttgg aaagtcaatg gcgtggcaaa cgaccgcctg tccaaaatcg gcaaaggcac
     3601 gctgcacgtt caagccaaag gggaaaacca aggctcgatc agcgtgggcg acggtaaagt
     3661 tattttagat caacaagcag atgaaaataa taaaaaacaa gcctttagtg aaatcggctt
     3721 ggtcagcggc agggggacgg tgcaactgaa tgccgataat cagttcaacc ccgacaaact
     3781 ctatttcggc tttcgcggcg gacgtttgga tttgaacggg cattcgcttt cgttccaccg
     3841 tattcaaaat accgatgaag gggcgatgat tgtcaaccac aatcaagaca aagaatccac
     3901 cgttaccatt acaggcaata aagatattgc tacaaccggc aataacaaca gcttggatag
     3961 caaaaaagaa attgcctaca acggttggtt tggcgagaaa gatacgacca aaacgaacgg
     4021 gcggctcaac cttgtttacc agcccgccgc agaagaccgc accctgctgc tttccggcgg
     4081 aacaaattta aacggtaaca tcacgcaaac aaacggcaaa ctgtttttca gcggcagacc
     4141 gacaccgcac gcctacaatc atttaggaag cgggtggtca aaaatggaag gtatcccaca
     4201 aggagaaatc gtgtgggaca acgactggat caaccgcacg tttaaagcgg aaaatttcca
     4261 tattcagggc gggcaggcgg tgatttcccg caatgttgcc aaagtggaag gcgattggca
     4321 tttgagcaat cacgcccaag cagtttttgg tgtcgcaccg catcaaagcc acacaatctg
     4381 tacacgttcg gactggacgg gtctgacaaa ttgtgtcgaa aaaaccatta ccgacgataa
     4441 agtgattgct tcattgacta agaccgacat cagcggcaat gtcagccttg ccgatcacgc
     4501 tcatttaaat ctcacagggc ttgccacact caacggcaat cttagtgcaa atggcgatac
     4561 acgttataca gtcagccaca acgccaccca aaacggcgac cttagcctcg tgggcaatgc
     4621 ccaagcaaca tttaatcaag ccacattaaa cggcaacaca tcggcttcgg gcaatgcttc
     4681 atttaatcta agcaacaacg ccgtacaaaa cggcagtctg acgctttccg acaacgctaa
     4741 ggcaaacgta agccattccg cactcaacgg taatgtctcc ctagccgata aggcagtatt
     4801 ccattttgaa agcagccgct ttaccggaca aatcagcggc agcaaggata cggcattaca
     4861 cttaaaagac agcgaatgga cgctgccgtc aggcacggaa ttaggcaatt taaaccttga
     4921 caacgccacc attacactca attccgccta tcgccacgat gctgcaggcg cgcaaaccgg
     4981 cagtgtgtca gacacgccgc gccgccgttc gcgccgttcc ctattatccg ttacaccgcc
     5041 aacttcggta gaatcccgtt tcaacacgct gacggtaaac ggcaaattga acggtcaggg
     5101 aacattccgc tttatgtcgg aactcttcgg ctaccgaagc gacaaattga agctggcgga
     5161 aagttccgaa ggcacttaca ccttggcggt caacaatacc ggcaacgaac ccgtaagcct
     5221 cgatcaattg acggtagtgg aagggaaaga caacaaaccg ctgtccgaaa accttaattt
     5281 caccctgcaa aacgaacacg tcgatgccgg cgcgtggcgt taccaactca tccgcaaaga
     5341 cggcgagttc cgcctgcata atccggtcaa agaacaagag ctttccgaca aactcggcaa
     5401 ggcagaagcc aaaaaacagg cgggaaaaga caacgcgcaa agccttgacg cgctgattgc
     5461 ggccgggcgc gatgccgtcg aaaagacaga aagcgttgcc gaaccggccc ggcaggcagg
     5521 cggggaaaat gtcggcatta tgcaggcgga ggaagagaaa aaacgggtgc aggtggataa
     5581 agacagcgcc ttggcgaaac agcgcgaagc ggaaacccag ccggctacca ccgccttccc
     5641 ccgcgcccgc cgcgcccgcc gggatttgcc gcaaccgcag ccccaaccgc aacctcaacc
     5701 ccaaccgcag cgcgacctga tcagccgtta tgccaatagc ggtttgagtg aattttccgc
     5761 cacgctcaac agcgttttcg ccgtacagga cgaattggac cgcgtgtttg ccgaagaccg
     5821 ccgcaacgcc gtttggacaa gcggcatccg ggacaccaaa cactaccgtt cgcaagattt
     5881 ccgcgcctac cgccaacaaa ccgacctgcg ccaaatcggt atgcagaaaa acctcggcag
     5941 cgggcgcgtc ggcatcctgt tttcgcacaa ccggaccgaa aacaccttcg acgacggcat
     6001 cggcaactcg gcacggcttg cccacggcgc cgttttcggg caatacggca tcggcaggtt
     6061 cgacatcggc atcagcacgg gcgcgggttt tagcagcggc agtctttcag acggcatcgg
     6121 aagcaaaatc cgccgccgcg tgctgcatta cggcattcag gcacgatacc gtgccggttt
     6181 cggcggattc ggcatcgaac cgcacatcgg cgcaacgcgc tatttcgtcc aaaaagcgga
     6241 ttaccgctac gaaaacgtca atatcgccac ccccggcctt gcgttcaacc gctaccgcgc
     6301 gggcattaag gcagattatt cattcaaacc ggcgcaacac atttccatca cgccttattt
     6361 gagcctgtcc tataccgatg ccgcttcggg caaagttcga acgcgcgtca ataccgccgt
     6421 attggctcag gatttcggca aaacccgcag tgcggaatgg ggcgtaaacg ccgaaatcaa
     6481 aggtttcacg ctgtccctcc acgctgccgc cgccaaaggc ccgcaactgg aagcgcaaca
     6541 cagcgcgggc atcaaattag gctaccgctg gtaaccgccg gatatgccga aaggggtctg
     6601 acgatgccac cgtgcgctgt caaacccttt ttctgccgcc gccttgtgcc tgctccattg
     6661 tctgataacc gtcaaattga tttttagccc atgtttggtg ggtcggggaa atctatatct
     6721 tcgtccgtgc cgaaatagtc
//
LOCUS       DAXHKX010000107         6523 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHKX010000107 DAXHKX010000000
VERSION     DAXHKX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.107"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(141..1529)
                     /locus_tag="ACFMWZ_001511"
     CDS             complement(141..1529)
                     /locus_tag="ACFMWZ_001511"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC5935457.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            1857..2804
                     /locus_tag="ACFMWZ_001512"
     CDS             1857..2804
                     /locus_tag="ACFMWZ_001512"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC5935458.1"
                     /translation="MIDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDQIHLQEYDTGWLMQPGEYEAWLAEVLEAVAFVTGFAPEQIHLKRRERQKGLQQ
                     YEKTGKAGDDFVIIENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAVSSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEDRQFDLIVMDPPSFSNSKKMPGILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="ACFMWZ_001513"
     CDS             complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="ACFMWZ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC5935459.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            3487..3971
                     /gene="dnrN"
                     /locus_tag="ACFMWZ_001514"
                     /pseudo
     CDS             3487..3971
                     /gene="dnrN"
                     /locus_tag="ACFMWZ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
     gene            complement(4041..4862)
                     /gene="nadE"
                     /locus_tag="ACFMWZ_001515"
     CDS             complement(4041..4862)
                     /gene="nadE"
                     /locus_tag="ACFMWZ_001515"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC5935460.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGAHQTAFDSQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMRHKINPIPVCRIPPELLG"
     gene            5059..6414
                     /gene="xseA"
                     /locus_tag="ACFMWZ_001516"
     CDS             5059..6414
                     /gene="xseA"
                     /locus_tag="ACFMWZ_001516"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC5935461.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTVVQGTGSELQIAQVIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKFDWLARQIRHPRQKLDEQRTYIHKLAQTLSYSMTQNLRA
                     HAARFERQTQALQHYRPDISVCKKNIDRFQTALPAAFSQLLARRRQSLSAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         1487 a         1726 c         1762 g         1548 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatattgc agattaatcc tttgggtatc aaagccgggc
       61 ggtgttgaaa cattgcaaaa atgccgtctg aaactcgaaa tcggtttcag acggcattgg
      121 tttatgaggc aggttggggc ttagcggtct ttgtaacggc gttcgctttt gcctgcaaaa
      181 gaatcgccgg ctttacgttt gcctttgaag ccttcgccgg tttttttgaa accgtctttc
      241 ttaaagcctt ctttcttgaa accttcgccg cgcgttttgc cgccgaagcc ttctttgccc
      301 ggtttatgat cgccgcgcca accgccggat ttacgatcgc cccagccgcc tttgcctttc
      361 ggcttgccgc ctgcggattt gcgtttgcgg gtcggttcca tgccctcgat ggtcagttcg
      421 ggcagttttc ggttaatgta tttttcgatt ttgtggactt tgacgtattc gttcacttcg
      481 gcaaacgtaa tcgcaatacc cgtgcggcct gcgcggccgg tgcgcccgat gcggtggacg
      541 tagtcttccg cctgtttcgg caggtcgtag ttgataacgt gggtaatggt cggtacgtcg
      601 ataccgcgtg cggctacatc ggtggcaacc aaaattttgc agcggccttt gcgcaagtcc
      661 atcagcgtgc ggttacgcca gccttgcggc atatcgccgt gcaggcagtt ggcggcgaaa
      721 cctttttcgt acaattcatc cgcgatgact tcggtcatgg ctttggtgga cgtgaaaatc
      781 acgcattggt cgatgttggc atcacgcagg atgtggtcga gcaggcggtt tttgtggcgc
      841 atatcgtcgc agtacagcag ttgttcttcg attttgcctt ggtcgtccac gcgttcgact
      901 tcgatgattt cagggtcttt ggtcagtttg cgtgccagtt tgccgaccgc gccgtcccaa
      961 gtggcggaga acaataaagt ctgacggtcg ctcggcgttg cttccacgat ggtttcgatg
     1021 tcgtcgataa agcccatatc caacatacgg tcggcttcgt ccagaatcag cacttccaaa
     1081 cgttcaaaat cgactttgcc gctttgcatc aagtccatca ggcggcccgg cgtggcgaca
     1141 atcagatcga ccggtttgct cagggcgcgg gtttggtagc cgaacgatgc gccgccgacg
     1201 atgctgacgg tacggaacca acgcatattt ttggcatacg ccagcgcgtt tttctcgact
     1261 tgagccgcca attcgcgggt cggggtcaac accaaagcac gcgggccttt gcccggtttt
     1321 tcgctgcgtt tggtcagttt ttgcaaagtc ggtaacagaa aggcggcggt tttgccggag
     1381 ccggtttgcg ccgaagccat gatgtcgcgg ccttccaaag caaacggaat cgcctgcgcc
     1441 tgaatcggcg tcgggctttc gtaaccctcg ctgctgacgg cggacaaaat gtttttatca
     1501 aggttcaaat cggcaaattt aatagacatg gctatccttg gaagacaaca acgcccgcgt
     1561 ccgaaaggtt cagacggcct gaagcaggga agggtaacga tacggggaat gtgaagttac
     1621 agggttgtcg caagcggctt gcttgtggtt tacatcgacg gcaacctgca cagatacgct
     1681 ttgcaaaatc aaagcaacag aagaaacgcg cccgtaaggg cggtttaggt tggaaggcga
     1741 tggcttcgta tgacaagata agcgacggat ggtatagggt ttgcccgaaa cggtcaagca
     1801 atcgggcgtt aattacagta aaattgccgt tttccacttt attccgcctg aaaccaatga
     1861 tcgacatcac accgtttgcc aaccgcttgg gcaaaaacat caaacacctt atgaaatggg
     1921 cgaaacgcaa cggcatcgaa gcctggcgca tttacgaccg cgacatccca caattcccct
     1981 tcgccgccga tgtttacggc gaccaaatcc accttcagga atacgatacc ggctggctga
     2041 tgcagcccgg agaatacgaa gcgtggcttg ccgaagtatt ggaagccgtc gccttcgtta
     2101 ccggttttgc gcccgaacaa atccatctca aacgccgcga acgccaaaaa ggtttgcagc
     2161 aatacgaaaa aaccggcaag gcgggcgacg atttcgtcat catcgaaaac ggccgcaagt
     2221 tttgggtcaa ccttgataag tatttggata cggggctttt tttggatcat cgaaacacgc
     2281 gcaaaaaagt cggcgaaacg gcggcgggca aacgttttct caacctgttt tcctacaccg
     2341 gcagcttcac cgtctatgcc gccaccggcg gcgcggtatc cagcgaaacc gtcgatttgt
     2401 ccaacactta tctcgattgg gcgaaacgca attttgaatt gaacggcatc gatacggaac
     2461 gacacaaaat cgtccgcgcc gacgtgttcc aatatcttca gacggcatat ggggaagaca
     2521 ggcagttcga cctcatcgtg atggacccgc ccagtttttc caacagcaag aaaatgcccg
     2581 gcatcctcga catccagcgc gaccacaaaa agctgattga cggcgcggtg aagctgctcg
     2641 cttcagacgg cattttgtac ttttccaaca acctgcgcag cttcgtctta gacgacttgg
     2701 tatcggaaca atacgccgtc aaagacattt ccaaacaatc cgttcccgaa gatttccgca
     2761 acaaaaaaat ccataggtgc tgggaaatcc gccacaaatc ttaagggtgc gaaaaagccg
     2821 ccggaatctt ccggcggctt tttcgattgc gctttatcag gcgatagagg cttcgacaaa
     2881 ggcggtcagc tgaccttttg ccaatgcgcc gactttggtg gcgacgactt cgccgttttt
     2941 gaacaccatc agggtcggaa tgccgcgcac gccgaaacgg gacggggtgg cttcgttgtc
     3001 gtcgatgttg attttgacca ctttcagacg gccttcaaat tcggcggcaa tgtcgtccaa
     3061 aatcggggca atcattttgc aggggccgca ccacggagcc caaaagtcca gcaggacggg
     3121 gatatctgca tttaaaacgt ctttttcaaa agcggcatcg ctggtgtgta caatcaattc
     3181 gctgctcatt atttttcctt ttcggttggg attggcacga tagcgtgcag gatagggctt
     3241 gggcataaaa atttcaagtg ggcgggcggg ataatagttt ttctgtaccg ccccaatcgc
     3301 ctttgcaaat tctaaaaaac ggttcggaca gtattttatt tctgaaaatt acttttatta
     3361 tcaaaatgta aggaatacgt ttgaggctgt acagaagcgc gcgggcggtg tatgctccgt
     3421 ttgaattgat tcttaaagta tgaatgttca aaaccaaacc cgaaaccctg taaaaggaac
     3481 actgcgatga ctgatttttc cgtttgggag accgccccct ttggtgcaac cgtcgatcat
     3541 atcctgcaac gctaccacaa tgtccaccgc gcacaatttg aagaattggt gccgctggcg
     3601 cagaaagtgg cccaagtcca tgccgatacc tttccggtac ctttccggca gaaattgccg
     3661 ggctgcttgc ctatatgcag aacgaactgc tgatgcatat gatgaaggag gagcggatgc
     3721 tgtttccgat gattaatcag ggtgtcggac gcggtgcggc gatgcccatc ggcgtgatga
     3781 tgcacgaaca cgaagaacac gaccgcgcca tcgcacggct caaagagctg accgacaatt
     3841 tccaaccgcc agaaggggct tgcggaagct ggacgcggct ttacgcgctg gcgaaagaaa
     3901 tggtggagga tttgaacgac cacattcatt tggaaaacga tattttgttt gcccgcgtac
     3961 tcgattctta agggatagga gtcggatatt tccatgccgt ctgaaatacg gttttccgtt
     4021 tcagacggca tttccgtgtt tcagcccagc aattcgggcg gaatgcggca tacgggaatc
     4081 gggttgattt tgtgccgcat ggcgcggtga agtcgcgtat agatttctag aacttcgcgc
     4141 tgccgccctt caaaatcttc gggtttgcgc gtgccgtaca cgcccattgc ccactccagt
     4201 tcgggatagc ttgcgcccat ctgttcttcg tcggtgcgtt ccgtatccca caggccgtcg
     4261 gtcggcgggg ctttttgaat cgcctcgtcc acgcccaatg cttcggcaag ccggtaaacc
     4321 tgcgttttgg tcaggtcggc aatcgggctg atgtccacgc cgccgtcgcc gtatttagta
     4381 aaaaagccca cgccgaaatc ttcaatctta ttacctgtcc ccgtaaccag cagtccgtgt
     4441 atctgcccgt agtagtacag ggtcagcata cgtaggcggc ttctggcgtt ggcgagggaa
     4501 agcggctgac tgtcaaatgc cgtctgatga gcaccgacgg tttgttcaaa ggtctggaag
     4561 gtgtcggtca ggtcgaccgt ttgcgcgctt acattggcat attgccgttg cagattgcgg
     4621 atgtgcagcc ttgcccgctc aagctggccg gggtgttggc gtatcggcat atccagaagc
     4681 agcgtggggc ggccggtgcg ggcggcgagt gcggagacga cggcggaatc gatgccgccg
     4741 gaaacgccca cgacaaaccc ttttgcattt gcccgggcgg cgtattcgtc caaccatcgg
     4801 gcgatatgtg tgataactgc ctgcgtatcc atttttcctc ctgtcgaaaa tatccgcatc
     4861 atagcctgcc ggcggtgcaa tttgaataga ttgcaggcag gttgatgaat atttttccca
     4921 ttccccgctt aaaaaagtca tacgggaaaa ggcgggcggc aagatggcgc gtgttataat
     4981 attgttgaca atttaggcgg cccgatgccg ccttgggctt caaaacatat tcatattttt
     5041 tattatttga ttgattcgat gtctgatttt tttcattctg acgtgttgtc cgtttccgag
     5101 ttgaatgcat ttgccaaaag tattttggaa aaccaccttg ccggtttgtg gattgccggc
     5161 gaagtgtcca acctgacccg cgccgccagc ggacattatt acttctcgct taaagacagc
     5221 cgcgcgcagg tgcgttgcgc gatgttcaaa ggtgcggcgg cgcgtttggc gaagcctttg
     5281 aaagaaggcg atcatatcga ggtatcagga aaaatcagta tttacgaggc gcggggcgaa
     5341 tttcaaatta ccgtaaacga agtacggctc aaaggtttgg ggcagcttta cgaagcctat
     5401 gagcggttga aggcgcagtt gcaggcggaa ggcgcgtttg cggcggaacg caaaaaacct
     5461 ctgcctgccc gaccgcaatg tatcggcatc gtaaccagtt tggcggcggc ggcattgcgc
     5521 gatgttgtaa ccaccttgaa acgccgcgcg cccgaaattc ccgtcatcgt ttatccgacc
     5581 gtcgttcaag gcacaggcag cgagttgcaa atcgcacaag tgattaaaac cgcatcgcaa
     5641 cgcgccgaat gcgatgtgtt gattgtctgt cgcggcggcg gcagcattga agatttgtgg
     5701 gcgtttaacg aagaaccggt cgtacgcgca atcgaagcct gcacgattcc ggtcgtcagc
     5761 ggcgtaggcc acgaaaccga tttcacgctt gccgattttg ttgccgacgt acgcgcaccg
     5821 acaccgaccg gtgcggcgga gctggtcagc cccaaccgcc aagaatcgct acatcgtctc
     5881 gctcaggcgc aaggccgtct gaaaaccgtt ttggaacaac gctatttcga tgccagccaa
     5941 aaattcgact ggctggcgcg gcaaatccgt catccgcgcc aaaaactcga cgaacagcgc
     6001 acctacatcc acaaactggc gcaaacgctg tcttactcga tgacgcaaaa cctccgcgcc
     6061 cacgccgccc gtttcgaacg ccaaacccaa gccctgcaac attaccgtcc tgatatttcc
     6121 gtttgcaaaa aaaacatcga ccgctttcag acggcattgc ccgccgcctt cagccaattg
     6181 ctcgcccgcc gccgccaaag cctgagcgcc caagcagcat tgctcgaagc cgtttcgccg
     6241 cagcacattc tggagcgcgg cttttccgtc gtcaaaaaca cacgcggaca agtcatccgc
     6301 aacgccgatg ttttgaagca ggggcagaag ctgcacatta tcttcgccga cggcgaaacc
     6361 gacgtgcgcg taagcaaaga acaagggcag caggatttgt tcgattgcat ttaaacccaa
     6421 tcggaaaaac cgctctcccg ccgccgtcat tcccgcgaaa gcgggagtcc ggaagcggaa
     6481 agccgccggg gtttatcaga aatgactgaa attcaaaaaa ccg
//
LOCUS       DAXHKX010000108         6499 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHKX010000108 DAXHKX010000000
VERSION     DAXHKX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.108"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(137..1036)
                     /locus_tag="ACFMWZ_001517"
     CDS             complement(137..1036)
                     /locus_tag="ACFMWZ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFC5935462.1"
                     /translation="MPALTVTWMIYTKYLVYQIYFVIFCGVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            1002..1331
                     /locus_tag="ACFMWZ_001518"
     CDS             1002..1331
                     /locus_tag="ACFMWZ_001518"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC5935463.1"
                     /translation="MYIIQVTVKAGIKACKVSNGFYHGGIMQANEIYKSLEEKNISAR
                     MLASALGVTNQSVSEVIRNGRGSKRIAEAIAKVIQKDLVEVFPHYKKTDCRDKKIAEL
                     KEMLGVG"
     gene            1333..3309
                     /locus_tag="ACFMWZ_001519"
     CDS             1333..3309
                     /locus_tag="ACFMWZ_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC5935464.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPIEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DVRCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRSAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGSGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASVVNVFDMD
                     GKFITKAQADGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALEQAAAW
                     DELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADM"
     gene            complement(3376..3453)
                     /locus_tag="ACFMWZ_001520"
                     /pseudo
     CDS             complement(3376..3453)
                     /locus_tag="ACFMWZ_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            3524..4438
                     /locus_tag="ACFMWZ_001521"
     CDS             3524..4438
                     /locus_tag="ACFMWZ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC5935465.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASVSMYLNGT
                     YAEKGGNYETIETKIEAFLEMQESKARREEPVFGFVSTKTTRRIAEAMRNAHEGGEIA
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSERLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKYG
                     ELVQLHSRVAGALNLGDTMPDAELEQIARATLPEADDETIAELVKHSNGNTRRMSKLM
                     RGSVRVANKNGIRMQAGIVKKYSSLIIR"
     gene            4454..4696
                     /locus_tag="ACFMWZ_001522"
     CDS             4454..4696
                     /locus_tag="ACFMWZ_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFC5935466.1"
                     /translation="MRHEYAVHAGVYEDTWHDYETHKRRKIWRADIRGKRKEGFAWLQ
                     IRRLRKRFESKEEAKEWAAQVKADWVRNNFFALRKY"
     gene            4724..4948
                     /locus_tag="ACFMWZ_001523"
     CDS             4724..4948
                     /locus_tag="ACFMWZ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935467.1"
                     /translation="MSNLFCKRMLSGAAVLVWAVFFISMLRGCAPELSEAKLEMSRRE
                     LLADLEAKARIEQYEAMSTEEKMKGIVYER"
     gene            5054..5209
                     /locus_tag="ACFMWZ_001524"
     CDS             5054..5209
                     /locus_tag="ACFMWZ_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935468.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKNREREIEEYQARFRRPV
                     DKKEEKK"
     gene            5206..5328
                     /locus_tag="ACFMWZ_001525"
     CDS             5206..5328
                     /locus_tag="ACFMWZ_001525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935469.1"
                     /translation="MIWFVVGLAVLVLLGIWLELLARIVVLHMIGEGHDGYDDN"
     gene            5408..5575
                     /locus_tag="ACFMWZ_001526"
     CDS             5408..5575
                     /locus_tag="ACFMWZ_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935470.1"
                     /translation="MNAKEIAEWLEDRGELMLMKKDGEGFVIAARSPDGMWKTAEAET
                     LARAINLWEEV"
     gene            5639..5866
                     /locus_tag="ACFMWZ_001527"
     CDS             5639..5866
                     /locus_tag="ACFMWZ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935471.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            5881..6399
                     /locus_tag="ACFMWZ_001528"
     CDS             5881..6399
                     /locus_tag="ACFMWZ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFC5935472.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
BASE COUNT         1841 a         1270 c         1822 g         1566 t
ORIGIN      
        1 actgaacatg gaacttgtat aatagttcca tgtttgaaaa aatcttttaa tatcatacat
       61 ctaaaaaaac aaagcatctg aagaccataa gctaaacatg aaacttgaat ttaaaggcgg
      121 agtttcatgt ttaagcctat tttttccgtt ttttagtcga tactgctgca tatgtttttt
      181 gcaaatcttc atcacttaat tcagtcaggc tttctactga gaaatttttt tcgaggtaac
      241 ttttcaacca atcttcaagc tcaagctgtt ttacatttaa tttgatataa gcgtatttct
      301 tttttcgcca gtcgggatcg ttgttctttg atgttgctgt attgctcaac ctacccagcc
      361 attttctgag ataagtttct gctttgtcgt aatcgctcaa tgcgattagt ttgtaacttg
      421 gaactttaca gtgagcattc agactagccc aaattgcacg gattgatttt ggcgaccgtt
      481 tggcaatctc ttctgctgcg gcaacttgtt taacaagttg ttgcagcttt gaagcttgct
      541 caagcgttat atgttccttg ccgggttttg aatcgacacg ggttctgatg acgtgatttt
      601 gggtattaat ttgatgaaca accgaaccag atgaggcgat aacagcgtta ctgacgttgc
      661 cgttaatctg attctcaacg tttgatttgc caatagcgtc gtccaatttt ttattaaggt
      721 cgtctgaaac agcatttaaa tttgccgaat gaatgcctgt aaagacaaat tgaatatcca
      781 cccctaattg gacagcagca agtaaaaagt ccgcaggaat gttgacctgc ccgctttcat
      841 acagtctcaa actttctgca ctacatccag tttgctcagc aaaattcttt cgagagtatc
      901 caagtcggct tctctcttca actaaattca atgctaaatc aatgcgttcc ataaaaacac
      961 cgcaaaatat tacaaaatat atttggtata ccaaatattt tgtgtatatc atccaagtaa
     1021 cagttaaggc aggcattaaa gcctgtaagg taagtaatgg attctatcat ggaggcatta
     1081 tgcaagcgaa cgaaatctat aaatccttag aagaaaaaaa tatatcggca agaatgttgg
     1141 cttcggcttt aggggtaact aaccaatctg tttctgaggt cataagaaat ggacgaggaa
     1201 gcaaaagaat tgcggaagca atcgcgaaag tgattcaaaa agatttagtt gaagtttttc
     1261 cgcattacaa gaaaacagat tgtcgagaca aaaagatagc tgaattgaaa gagatgctcg
     1321 gagtgggttg aaatggaata tatggatctc aaaagcctgc ttaattttgg gttgccaaat
     1381 atgccaagaa cagtaatagg gctgagaaaa aaagcgcaaa gggatggttg gcaaacgaga
     1441 aggcgtcaag gtaagggtgg tggtgttgaa tatgccctac ctattgaaat ccgagcagcc
     1501 atcatgaaac ggcagtcgga cgagctggcg gagaagatgc cgaaaatgct gccccaagtc
     1561 agaccgggga cggcgatgtc ggctcaggca ctggctgaag cggccaagct gttgaacgag
     1621 aaacaacggt cggtggcgga tgtgcgatgt gcggtggtgg cggcggtgtt ggggattaaa
     1681 tacgaatacg attgctctgc caaggctgcg gtggctcagt ttttgggctt gctggcagaa
     1741 ggtaaattgg acgcggtcac gcttggtaac ttggaaaagg ccaatgaccg cagccggtcg
     1801 gcgaaggttg gcgaacgtac tttagacggc tggatttctg cttatttgaa agcggaaaac
     1861 gcgacggagc ggttggttgc tttggctccg aagacgacga aggcggtcaa gccgattgag
     1921 agctacggtt ggttgccgat gtttatgcag tttcacaaca tcccttcggc tccgaaactg
     1981 gcacatagct accgccggtt tgtgcagtgg gcggaagcgg aaaatatgcc ggttaacgat
     2041 gtgcctaact tgagtatggt gcggcgcgtt tgggacaagc tcccgttgat tatgcaggag
     2101 cgtggcagga aaacgggggc ggcttataaa tcgctgctgc cttacgtgaa acgtgattgg
     2161 ggggctttga agccgaacga tgtttggatc ggcgacggcc acagctttaa ggcgaaggtg
     2221 gcgcaccctg tacacggcag gccgtttaag ccggaagtga cggtgattat tgatggttgt
     2281 acgcggtttg tggtcggttt ttcggtttcg ttggctgaaa gttgtgtggc ggtatcggat
     2341 gctctgcgta ttggggtcaa gcattttggt ttgccgatca tctactactc ggataacggc
     2401 ggcggccaaa ccggcaagac gatagaccat gaaatcacgg gtattacgtc ccgactgggt
     2461 atccggcatg aaacgggtat cgcgggcaac ccgcaagggc gcggcatcat tgagcgatgg
     2521 tggaaagaca atctgattga gatggcgcgg cagtatgaga cgtttgcggg cagcgggatg
     2581 gacagcagca cgaagaatct gatgtaccgc aagatggaaa gtgcttttaa tgctttggaa
     2641 aaaggcaagg atttgacgga ggaacaacag aaatatttga aaaaactgcc gagctggtcg
     2701 cgttttatcg cggatgtggt caagtgtatc gacgaataca acaaccgccc gcacggcgag
     2761 ctgccccgac atcctgacgg cgggcattat acgccgaagg cttatcggga aatgaggctg
     2821 gaacaggacg gtatcgcgcc ggatatgttg tcggcgcaag agctggcgac gatgtttatg
     2881 ccgcaagagg tgcgaaaggt acagcgcggt tggctggatt tgttcaacaa ctcttatttc
     2941 tcaaccgagc tggcggagta tcacaaggac gaggtacggg tcagctacga tttgagcgat
     3001 gcgtcggtgg tcaatgtgtt tgatatggac ggcaagttta tcactaaggc gcaggccgac
     3061 ggcaataccc gcgaggcttt cccgacggcg cgtatcgacc aactggcgga aaaccgccga
     3121 aaaggcaaaa tcaagcgggc ggaaaatgca atcaagctcg caaatgcgga agtcaatccg
     3181 gcactggaac aggcggcagc ttgggacgag ctgggacatt tgggcggaaa cgtcatcgag
     3241 gcggagtatg cggtattgcc gaaaacgggc acggacgatg ggattttctt gtttgaggcg
     3301 gatatgtaaa ggaaaacatg atggacaaac agcaaaatgc ggcgttttcg gccgagcttg
     3361 ttgaaaaatt gaaactcaag cgagcccttg ggcggattca acgatttcaa gcaaagattc
     3421 aaggtgttcc cgctgaacgg aatcaggctc aaatttctaa atcgggaaac tcttttattt
     3481 cagacggctt ttaaaggttg tttaaaactc aaggatatta aaaatgaaac aaattaatca
     3541 agcattgcaa caaaaactgg ttgaatttaa agaaaaatcg ggtatgaacc aaacccaact
     3601 ggcacgcggt atcgatactt cgccggcatc cgtcagtatg tatctgaacg gtacttatgc
     3661 ggaaaaaggc ggcaattatg aaaccatcga gacgaaaatc gaggcgtttt tggagatgca
     3721 ggaaagtaag gcgcgacgtg aagagccggt gtttggtttt gtatcgacta aaacgacccg
     3781 ccgaatcgcg gaagcgatgc gcaatgcgca cgaaggcggc gagattgccg ttatctacgg
     3841 gcaggcgggt ttgggcaaga cgcaggcggt caaaaactac tgcgagaaaa accctgcagc
     3901 catcttaatt gaggctaatc cgagctttac ggctttggtc ttgatgcgca agttggcgac
     3961 ggcggcgaag gtatcggcga tgggcagcct gaatgatttg tttgaatcgg tatcggacag
     4021 attgcgtgat tcggagcgtc tgattgtggt cgatgaggcg gaaaacctgc ccttgcgcgc
     4081 ccttgagatt atccgccgac tgcacgatga gactggctgc ggtttggtgt tgagcggtat
     4141 gccccgactg gtggcgaatt tgcgcggtaa gtatggcgag ttggtacagc ttcacagtcg
     4201 tgtggcgggt gcgctgaatc taggtgatac gatgcccgac gcagagttgg aacagattgc
     4261 gcgggcaacc ctacccgaag ccgatgatga aacgattgcg gaactggtta agcatagcaa
     4321 tggtaataca cggcgtatga gcaagttgat gcgcggatca gtacgggtag cgaataaaaa
     4381 cggtattcga atgcaagcgg gaatcgttaa gaaatacagc tccctgatta tccgataaga
     4441 aaggccgtct gaaatgagac acgaatatgc ggtacacgcc ggagtctatg aggacacttg
     4501 gcacgactac gaaacccata aacgtcgcaa gatttggcgg gcggatatac gcggcaagcg
     4561 gaaagaaggc ttcgcatggt tgcaaatccg ccgactgcgg aagcgatttg aaagcaaaga
     4621 ggaagccaag gaatgggcgg ctcaagtgaa ggcggattgg gtacgcaata atttttttgc
     4681 cttgagaaaa tattaattaa ttgatttata aggaaataga aaaatgtcta atttgttttg
     4741 caagcggatg ttgtcgggag cggctgtgtt ggtttgggct gtgtttttca tttcgatgtt
     4801 gcgcggttgt gctccggaac tatcggaagc aaagttggaa atgtcgcgcc gcgagcttct
     4861 ggcggatttg gaggcaaaag cccggatcga acaatacgag gcgatgagta cggaagaaaa
     4921 aatgaaaggg attgtttatg agcgataagc caattttatt gaaccctgcg gcgaaacaag
     4981 aggctttgga acgtgcgctt aaagaaatcc gcgcgaaata tggcgataag gcgattgtga
     5041 aaggatgtgt gaaatgagtt tcggacgacg taatacggat tggcaggctt ggggacaaca
     5101 ccgcaggcgt gcgacggcgc gaatggcgca aaaaaacaga gagcgtgaaa tcgaagaata
     5161 tcaggcgcgt tttagacggc ctgttgacaa gaaggaggag aaaaaatgat ttggtttgtt
     5221 gtcggattgg cggtgttggt gctgctgggg atttggcttg agctgttggc ccgaatcgtc
     5281 gtgttgcaca tgataggcga aggccatgac gggtatgacg acaattaaaa cggtaagccg
     5341 ttgatgttgc tctatatttt tttgccttgt tgaaaatata agatattgat ttaaaaggat
     5401 ttaagaaatg aatgcaaaag aaattgcaga atggctcgaa gaccgtggcg agctgatgtt
     5461 gatgaagaag gacggcgaag gctttgtcat cgctgcgcgg tcgccggatg ggatgtggaa
     5521 gactgccgag gcggaaactt tggctcgggc gataaattta tgggaggaag tgtgatgttt
     5581 aagtttttga gaaaccgcgc gaaagttgaa gcaattttaa acaagaaagg aaagtgaaat
     5641 gaaagtgtat gtttttaaaa ttagtaatga aaacggtaag ttaaagatag aactccccga
     5701 aatcccaatg ggtaagcaaa ttgacgaagt tgatttgatt gctgggctaa ccacggaatt
     5761 tattgcaagt atgttacgtg atgcccaaaa agatcgtcgc aaattcgtaa tcgacgcatc
     5821 aaatcaattg gctgcaatcc agacatatca aaaaatcttc aattaaggaa ggaagagaaa
     5881 atggctaaaa cccgaatcaa acagcccgcc atcgaagcgg cacaagacaa aacggaagtt
     5941 actgcattta tccgccaaat cggcgacttg cagcgcgaag tcaaacgcct ggaaaccgaa
     6001 gccggagaca aaaaagcggc tatcgaagaa gaatatgccg ccaaagccgc gccgatgtgt
     6061 gctgaaatca tgagcctgac cgaacgtgtg gccgcctact gcgaagcgca taaggacgag
     6121 ctgacggaaa atggtaaaac caaaaccgtg gactttacta ccggcctgat taaatggcgc
     6181 attcgtccgc catccgtcaa ggtaacgggc gtggcggccg tcttggcgtg gctctcggaa
     6241 aaatccgcct ttgccgagtt tgtccgcact aaaaaggaaa tcgacaaaga cgccatcctg
     6301 aatcaaaaag agcgtttttc agacggccaa gttccgggga ttaagattgt gagcggcgtg
     6361 gaggattttg tgattgagcc gagcgaacaa attctataaa atcaacagcc tgatttcaat
     6421 tcagtgtgtt gaaacgtgat tgagcctacg gagcaggagc ttgcctagcc ggttcaaata
     6481 ggctttaacc catgattta
//
LOCUS       DAXHKX010000109         6498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHKX010000109 DAXHKX010000000
VERSION     DAXHKX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.109"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..336
                     /locus_tag="ACFMWZ_001529"
     CDS             <1..336
                     /locus_tag="ACFMWZ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC5935473.1"
                     /translation="IDASGLGEKIKDGKKTVLSCEEEQKICNTFTHKQAVEDFSVVIG
                     YDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKD
                     RLAMLKFNS"
     gene            368..484
                     /locus_tag="ACFMWZ_001530"
     CDS             368..484
                     /locus_tag="ACFMWZ_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC5935474.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(644..1981)
                     /gene="ribBA"
                     /locus_tag="ACFMWZ_001531"
     CDS             complement(644..1981)
                     /gene="ribBA"
                     /locus_tag="ACFMWZ_001531"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC5935475.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKVGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            2090..2224
                     /locus_tag="ACFMWZ_001532"
     CDS             2090..2224
                     /locus_tag="ACFMWZ_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935476.1"
                     /translation="MPSEAGFVASDGIAAILAGRGLFVPTSRALLIWGLPEAYSEKNF
                     "
     gene            complement(2203..2859)
                     /locus_tag="ACFMWZ_001533"
     CDS             complement(2203..2859)
                     /locus_tag="ACFMWZ_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFC5935477.1"
                     /translation="MILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIANAWTK
                     IVRREIIIKNNLFFPKGYIHEDFPYSLKLARFIKTFAFYDNPFYQYRVLGGSISHNIK
                     YKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNIILIY
                     RKYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFFSE
                     "
     gene            complement(2891..3217)
                     /locus_tag="ACFMWZ_001534"
     CDS             complement(2891..3217)
                     /locus_tag="ACFMWZ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC5935478.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     RGGDSL"
     gene            complement(3385..3978)
                     /gene="ribA"
                     /locus_tag="ACFMWZ_001535"
     CDS             complement(3385..3978)
                     /gene="ribA"
                     /locus_tag="ACFMWZ_001535"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC5935479.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3971..4648)
                     /locus_tag="ACFMWZ_001536"
     CDS             complement(3971..4648)
                     /locus_tag="ACFMWZ_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935480.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMGMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVQSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            4802..4886
                     /locus_tag="ACFMWZ_001537"
     tRNA            4802..4886
                     /locus_tag="ACFMWZ_001537"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4836..4838,aa:Leu,seq:tag)
     gene            5406..6440
                     /gene="purM"
                     /locus_tag="ACFMWZ_001538"
     CDS             5406..6440
                     /gene="purM"
                     /locus_tag="ACFMWZ_001538"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC5935481.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGELDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGCIRER
                     QGDEHQTQVA"
BASE COUNT         1602 a         1589 c         1699 g         1608 t
ORIGIN      
        1 atcgacgcat cgggcttggg cgaaaaaatt aaagacggca aaaaaaccgt actttcctgc
       61 gaagaagaac aaaaaatctg caatactttc acgcacaaac aagcagtgga agatttcagc
      121 gtggtgatcg gctacgatga aatcaaagcg aagaatcaca gcttgtcggc ggggcagtat
      181 ttcgaggtaa agattgatta tgtggatatt tccgccgacg aatttgcgca aaaaatggcg
      241 ggattttcag cggatttgga taaacttttc gccgaatctg ccgaattgga aaaagagatt
      301 aaggatagat tggcgatgtt gaaattcaat tcataaatac cgatgaatga aaccggtcaa
      361 acttaaaatg ccgtctgaaa gcctttcaga cggcattttg tttggtttgg aaaatactta
      421 tgccgttctt tccctgaaag agagaatcca aaaaaccaaa gccacaggaa tttatcggaa
      481 atgacaaaaa cccgacgaac cggattcccg cctgcgcggg aatgacgaat tagaagttac
      541 ccgaaatttg aaaaaacaaa atccaaccca acagaccggg ttttcgtttg cacggaaata
      601 atgcaataaa taaagcaaat ataaagtatt tgaatttact atattatttt tccgcttctt
      661 caaagccgac gacttccaaa ccgaagccgg tcaggccggt gaaagatgag ggctgcccga
      721 ggacgcgcag ttttttgacg ttgaggccgg cgaggatttg tgcgccgatg ccgtagcttt
      781 tgctgtccca tttgtaggct tggtttgcgc ctttaggcag ggtgcggtcg agcagggatg
      841 cgccgtcttc ggtgcggtgc agaaggatga cgacgccgct ttctgcctgt tggacgcgct
      901 cgagtgcttt gggcaatgac catgaatggc gcgggttggc ttgaatgaag tccatcacac
      961 tgaagggttc gtggacgcgg acgagggttt cggtgtcggc ggcgggcgtg cctttgacga
     1021 gggcgaggtg ggtttcgccg gagagtttgt cgacgtaaac gtgttgttgg aactcgcccc
     1081 acggggtttg tacgggtgcg ttgcccatgt cttcaagcag gctttcggta cggctgcggt
     1141 attcgatgag gtcggtaatg gtgccgattt tgagcttgtg ttcttcggca aacttcatca
     1201 gttcgggcat acgcgccatc gtgccgtcgt cgttgatgat ttcgcaaata acggcggcag
     1261 gaatcagccc gttcatttgc gccaggtcga cgccggcttc ggtgtgtccg gcgcggacga
     1321 gtacgccgcc tttttgggcg cgaagcggaa agatatgacc gggttggacg atgtcttcgg
     1381 gtttggcggt cggggaaacg gcggtttgaa tagtcagggc gcggtcggcg gcggaaatgc
     1441 cggtggtaat gccgtgtgcg gcttcgatgg agacggtaaa gttggtgccg tattgcgcgc
     1501 cgtttttttg ggtcatcatc ggcagcccga gtttttcgac catttcgccg tccatcggca
     1561 ggcagaccaa gccgcgcgcg tgtttgatca tgaagttgat ggcttcgggc gtgacgaatt
     1621 gcgccgccat cagcaggtcg ccttcgtttt ctcggtcttc ggcatcggtg atgatgacca
     1681 ttttgccgac tttgatgtcg gctaggattt cggggatggg ggaaatatgg gacatggtgt
     1741 gttttcctgt gtgttcgggc gggcgggcgg tgtgttcggt atcgagccag aaggcgggca
     1801 tttttgccgc ctgttcgatt ttacgggctt ttttctcgcc gaaggatttg tttttcaaaa
     1861 gggcggagag ttcgcctgtg ttgagggcaa cggcttcggc aaaacgggtt tgcaaaccgc
     1921 cgtagtgttc tgctatccat tgccgcaggt tgcggcggcg cagaccggct gtgtcggtca
     1981 tcgtttcatc ctttgtagaa atcggatgta gcggattgta atcgcgtatt taccaaaaag
     2041 caaatctgtt tttttcgcca aaccgaatta tttgcttttt ggtaaacaga tgccgtctga
     2101 agccggattc gtggcttcag acggcattgc cgcaatcctt gccggccggg ggctgtttgt
     2161 cccaacttcc cgcgcacttc tgatttgggg tttgccggaa gcttattccg aaaaaaattt
     2221 ctgataaacg gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg
     2281 caatcctatg atgaatgcgg tcttcccgat aaaaaccgga cggattgcct tcgcgccgaa
     2341 tatctttctg catttttctt taaatgaaaa atatttccgg tagatgagga taatgttttt
     2401 ggaaaaataa agccttacca atatagacct cagatagccg atattgtcga agacaaattt
     2461 ttgcaaaccg ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc
     2521 gagatgcgtc agcacatcgc tgaaattttt gtatttgatg ttgtggctga tagagccgcc
     2581 gagaacgcgg tactggtaaa aagggttatc gtaaaaggca aaagtcttga taaaacgcgc
     2641 caatttcaaa ctgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt
     2701 tttgatgatt atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc
     2761 ctccaccagc gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg
     2821 gatgtcgcgg taattgaagg acgagggatg caggatcaaa tcaacctttt tgtctgcaag
     2881 ttgttgtaaa tcaaagagaa tccccccccc gcgcgttttt tgaacgattg gtgtcggccc
     2941 aatagtcgtc gctgtccaaa aagattaggt aatcgccttt tgccgcccgg ataccggcgt
     3001 tgcgggcatc cgacagcccg ccgttttctt gatgaatcac ttttatatgc ggatatttgc
     3061 ctgcatattc gtcgcaaatc ttcccgcagc cgtccggcga accgtcatcg accaaaatca
     3121 tttcataatc ggcaaaattt tcggcaagca cggaatccac gcagcagcgg aggtattttt
     3181 ccacattgta aatagggacg atgatggaga aaatcataaa tatcaatcca ttatattaag
     3241 atgtttgcgc gtatgcctca aacccgcacc cgcaatgcgt ttgcatccgc accctgtaac
     3301 tttataaaac ttattgcttt atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg
     3361 atgcccgttc cctgcatctt tgccttattc cgacatcaga tgccccagct tgtctgcttt
     3421 ggtttggagg tagcgtttgt tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac
     3481 cacgttaatc cccgcatctt tcagggtttg gattttttcg gggttgttgg tcagcagttt
     3541 gaccgagcgg atgcccagat attcgtagat agattgcgcc aaacggaaat cgcgggcatc
     3601 gacgggcagc ccgagtgcca aattggcttc aaccgtatcc ataccttggt cttgcagatg
     3661 ataggcgcgg attttgttaa tcagcccgat gccgcgtcct tcctgacgca gatagacgat
     3721 gatgccgcgc ccctctgtct gtaccgccct catggccgct tcaagttgag gtccgcagtc
     3781 gcattttctc gagaacagcg catcgcccgt caggcattcg gagtggatgc gcgtcagaac
     3841 cggattgccg tctgaaaaat taccgacggt cagcgcgacg tgttcctgcc cgtttgcctc
     3901 ttcaaagccg tgcatcgtaa atacgcccca ttcggtcggc aggcggcagg atgccacatg
     3961 gttcaacatt tcagacatct tcactcccat cttttccggc agacataccc aacagcagtt
     4021 tcaaaggttc ggacaaagcc aaggcaagag ccacccattc cgcctgcgct gcctcatccg
     4081 cacactctgc gcattcaaat tccacatgaa ccacgcccaa cacaccgcca ctttccgtgc
     4141 agaccggaat ggaaatttgc gccgccgaag catgattgcg ttctcccgaa agctccccca
     4201 aatccaacca acggcgtaca tccgaggcaa cattcatcca accgctttgc gccgaacggc
     4261 aagccagcga aaccttgcct gccgcttcat ccaaatccca caggttttcc aaaacctcgc
     4321 cctgtcggga caggcatatc agttggaaag cctggttttc agatggcatc aaggcataga
     4381 ccgccgcact ctgcacgcct gtcgagcgcg aaaacaccga atccaaagcc atgaaaagcc
     4441 gtttcagtgc atcttcgcgg acattgtcgc acgacaggta atcggcaagt ttccaaccct
     4501 catcactgcg ccacaaaacc gaacggtcta tcgaagccgt ccccataccc attaccgtct
     4561 gcgccgtcag atacgccgcc cgaacctcgt caagcggcag cttcaaaccc tgagtcagca
     4621 gaaaatcctt aatcaaagca gcaggcatac ccaaattccg tcaataaaaa caaaaaaccg
     4681 cccgattccg atgtcaggcg gacgaaaaac agattttacc gccccaaacc ccaagccatc
     4741 aacagccccg ccgaaaacct tgcacaaaaa aacaaaatct gtataatcgt ccaattccca
     4801 tgcggacgtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg agaggtgtga
     4861 gagttcgagt ctctccgtcc gcaccaataa tgataaataa attatatagt taatgcttat
     4921 cgggacaaat tagtgccaca aaacataagt cgtctgcttt tcagcgggcg gcttttttat
     4981 ctgcctatat gtttcaacac gcagaacggt acataaagtg ccgccatttg ttccgcaccg
     5041 aatcggaaac gggtgcttcc gcccgcaatc agattcaccg cccctaatgc cggggtttca
     5101 accggaaaga agaaaatatt gattttgatg atggcatcaa aaaacccctt ccgctcgtct
     5161 gccaaaagga atggttaaga aaagcaatgc gtcttttgat ggaacgcgat atataaggaa
     5221 ttgaggggaa gggcaggcgg gtttctgccc gttaatcctg cggacggtcc cgccaaaccc
     5281 gacacgcgcg ggtatatggc ggactgttta caatattgtc taaaaatctg aacctgcaaa
     5341 cgtgaaataa ggtagaatac gccccgttat tttaccgtcc aatccccatt taccaaggaa
     5401 aaacgatgag tacttcattg agttaccgcg atgcaggtgt cgatatcgac gcaggcgacc
     5461 aactggtcga aaacatcaaa ccgtttgcca aacgcaccat gcgtccggaa gtattggggg
     5521 atttgggcgg ttttggcgca ttggtcgaaa tcggcaagaa atataaaaat cccgtattgg
     5581 tcagcggtac ggacggcgtg ggtaccaagc tcaaacttgc ctttgattgg gataaacacg
     5641 atacggtggg catcgacctt gttgcaatga gtgtcaacga tattttggtt caaggagcag
     5701 aacctctgtt tttcttggat tatttcgcct gcggcgaatt ggatgttccg cgcgcaaccg
     5761 atgtcatcaa aggcattgca caaggttgcg aagaatccgg ctgcgccctg attggcgggg
     5821 aaactgccga aatgccgggt atgtatcccg tcggagaata cgatttggcg ggttttgccg
     5881 tcggcgtagt ggaaaaagag aatgtcatta ccggccgcag catcggcgcg ggcgatgtgg
     5941 tattgggttt ggcttccaac ggcgcacatt caaacggcta ttcccttatc cgtaaaatca
     6001 tcgaacgcga caatcccgat ttggatgccg agtttgataa tggcaaaacc ttgcgtgagg
     6061 ctgttattgc gccgacccgt ctgtatgtga aaccgattct tgccgctttg gaaaaattta
     6121 ccattaaagg tatggcacac attaccggcg gcggcattac cgaaaacgtg ccgcgcgtgt
     6181 tgcctgaaaa cacggttgca caaatcgatg ctaaatcgtg ggaattgccc aagctcttcc
     6241 aatggcttca aaaggcgggc aatgtggaaa cccaagaaat gtaccgaacc tttaactgcg
     6301 gcatcggcat ggtcgttatt gttgctgccg aagatgccga tgcggttcag ggtctcttgg
     6361 gtgaacaagg tgaaacagtt taccgtttgg gttgcatccg tgagcgtcag ggagacgagc
     6421 atcaaaccca ggttgcctga ttgcttctat agcgaaatgc cgtctcaaac atgggtttgg
     6481 acggcatttt tatagagg
//
LOCUS       DAXHKX010000110         6446 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHKX010000110 DAXHKX010000000
VERSION     DAXHKX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.110"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(98..1015)
                     /gene="thrB"
                     /locus_tag="ACFMWZ_001539"
     CDS             complement(98..1015)
                     /gene="thrB"
                     /locus_tag="ACFMWZ_001539"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC5935482.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLVAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="ACFMWZ_001540"
     CDS             complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="ACFMWZ_001540"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC5935483.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1932..3173
                     /locus_tag="ACFMWZ_001541"
     CDS             1932..3173
                     /locus_tag="ACFMWZ_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC5935484.1"
                     /translation="MPTKTPSLFGCAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGNALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWMGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3231..3929
                     /locus_tag="ACFMWZ_001542"
     CDS             3231..3929
                     /locus_tag="ACFMWZ_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC5935485.1"
                     /translation="MELKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     VAPPPQIRNASFHNPEKLPTCAPNTALPTMKPFSCSHRPATRAKVWNCLPTFSNIPAC
                     PSSSPLSAPRSPAL"
     gene            3812..4285
                     /locus_tag="ACFMWZ_001543"
     CDS             3812..4285
                     /locus_tag="ACFMWZ_001543"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC5935486.1"
                     /translation="MFPSTGHTRKGLELLADFFEHTSLPVKLAVVGSPLPRPMKNVVG
                     LGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGTRVVLSENMACTEIMNEEAGF
                     FFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYNPSLSHHIDRLTDMLASV"
     gene            complement(4352..6004)
                     /locus_tag="ACFMWZ_001544"
     CDS             complement(4352..6004)
                     /locus_tag="ACFMWZ_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFC5935487.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLDVPWRWAGAVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGLVRNLREHGYFCVVLSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTISALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIVH"
     gene            6174..>6446
                     /locus_tag="ACFMWZ_001545"
     CDS             6174..>6446
                     /locus_tag="ACFMWZ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFC5935488.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSDDFMGGGLTNPTVPSK"
BASE COUNT         1612 a         1970 c         1479 g         1385 t
ORIGIN      
        1 aaaatacccg gctatcgtct cattttcgtt ttaatcagcc ataaaaatgc cgtctgaaac
       61 cctttcagac ggcatttctg tcaaacgccg gacgcactca atccaaactc aacagcaggt
      121 tgcggaacgc gttcgggtct ttgataaacg tcatctcgcc cgcctgcgga aaatgaaagt
      181 ccaacaggcg cgacacccaa aaacggatgc agccggcacg ttgggcggtc gggaaatacg
      241 ccttttcttc ggcactcaag gggcgcacgc cctcataacc gccgataaac gcctttttca
      301 acgcctcatc caacttattg tccgccgtcc ttgcccaatc gttgaccgca atcgccaagt
      361 catacataaa attgccccgg caggcataat aaaaatcgat aaagcccgac acctgaccgc
      421 cgtcaagcaa cacattgtct ttaaacaaat cggcgtggat gatgcccgaa ggcagatgat
      481 tgccgagatt gtccttcagc gcatcgattt cggaacacag cagcgcagca tcgtcttgcg
      541 acaggacggg cagcagccgg gcgcacgcct ccgtccacca cgcatcgtaa cgcgggtttt
      601 ccatttccaa agggaaatcg gcggcgacaa ggtgcatttt ggccaacatc gcaccggtat
      661 ggaaacactg ctccgccgtc ggcagcgcag tatccgaacc tttcaggcag gcaaccaagc
      721 aggcgggctt gcccgccaaa acggaatcaa gccggccgtc tttgcgcgca accggcgcgg
      781 caaccgccac gcccttcata ctcaaatgcc ggttaagctc cagaaaaaac ggcagctctt
      841 cctgtttcaa cacttcaaac acggtcagca cataacgtcc cgaagtcgtc gtcagaaaat
      901 aattgctgtt ggtaatcccc tgcgcgatgc cctgcaggga aacaaattcc cccaaatcgt
      961 aaccgctcag gaagccgcgc atttcatcat cggaaacact ggtatagaca gacatatcaa
     1021 ctcacttgct caaaaacggg caaacccgcc gtcagaacgc cggacggcag gcaaacatat
     1081 aattcacatc ggtcgaatcg cacagggcat aagtttgcga caacacatgg taagtcatac
     1141 ccttcgtatc cgccacatcc aagcctgcct gacggcacat tcgcgccagc tcggcaggtg
     1201 cgatgaattt tttccagtcg tgcgtgcctt tggggacaaa cttcaacaga tattccgccg
     1261 ccacaatcag atgcaggtac gatttcgggt ttttattgat ggtagaaaaa aacaccatgc
     1321 cgtccggttt gaccagattg gcacaagcac gcacgatggc ggcgggatcg gggacgtgtt
     1381 ccatcatttc catgcacgtt accacatcga acgagtgcgg ttccgcctcg gcaaggtctt
     1441 ccacgcggat acattcgtat tcgatatcgg cgacattgtt caaagccgcg tgcaggcggg
     1501 cggtttccag cgactgctcc gccatgtcga taccctttac aaacgccgcg ccgcgccgcg
     1561 ccatgctttc cgccaggatg ccgccgccgc agcccacatc caaaacccgt ttgccgcaca
     1621 aatccgcgtg tccgtcgata taatccagcc gcagcggatt gatgtcgtgc aaggttttga
     1681 actcgcccga cttgtcccac catttgtcgg caatccggct gaatttggcg atttccccct
     1741 catcgacatt atattttttg tcggacattt tccctcccat ctgacgaacc gcccactcca
     1801 aaacccaaga ttatactatg gaacgcaatg ccgtctgaaa gccttttcgg gcggcgcggc
     1861 gcaaatacct tacaaatcct tacactttac ggcataatgg cggctcgctt tttctggcag
     1921 aaagacaaaa tatgcccacc aaaacccctt cactgttcgg ctgcgcgatg attatcgccg
     1981 gcacggtcat cggcgcaggt atgctcgcca acccgaccgc cacatccggc gtatggttta
     2041 ccggctcgct ggccgtgttg ctgtacacct ggttttccat gctctccagc ggcctgatga
     2101 ttttggaagt caacacccac tacccccacg gcgcgagctt cgacaccatg gttaaagacc
     2161 tgctcggacg cggctggaac atcatcaacg gtatcgccgt cgccttcgtt ttatacctgc
     2221 ttacttacgc ttatatcttc gtcggcggcg acctgaccgc caaaggcatc ggcaacgcct
     2281 tgggcggcaa tgtttcgctt accgtcggac aactcgtctt cttcggcatc ctcgcctttt
     2341 gcgtatgggc atccgcacgc ttggtcgacc gattcaccag cgtcctcatc ggcggcatgg
     2401 tattaacctt tatttgggca accggcggcc tgattgccga tgccaaaatg cctgttttgt
     2461 ttgacaccca agcccctgcc ggcaccggct actggattta cgccgccacc gccctgcccg
     2521 tctgcctcgc ttccttcggc ttccacggca acgtttccag cctgctcaaa tactttaaag
     2581 gcgacgcgcc caaagtggct aaatccatct ggatgggcac actgattgcg ctggtaattt
     2641 acgtcctctg gcaaaccgcc atccaaggca acctgccgcg caacgagttc gcccccgtga
     2701 ttgccgccga agggcaagtc tccgtcctca tcgaaaccct gtccaaattc gcccaaaccg
     2761 gcaatatgga caaaatattg tccctgtttt cctatatggc gatcgccacc tcgtttttag
     2821 gcgtaacgct cggactcttc gactacatcg cagacatctt caaatggaac gacagcgtgt
     2881 ccggccgcac caaaaccgcc gcgctgacct tcctgccgcc cctgatttcc tgcctgctct
     2941 tccccaccgg cttcgttacc gccatcggct acgtcggcct ggcggcaacc gtctggacag
     3001 gcatcatccc cgccatgctg ctctaccgtt cgcgcaaaaa attcggcgca ggcaaaacct
     3061 ataaagttta cggcggcttg tggctgatgg tttgggtctt ccttttcggc atcgtcaaca
     3121 tcgccgcaca ggtattgagc caaatggaac tcgtccccgt atttaaagga taaaggcaaa
     3181 atgccgtctg aagccctgtt cagacagcct tacgacattc ggaaaatcat atggaactca
     3241 aaatagacat tgcaaccaac aacttcaaac acggcggcgg cacggaacgc tacacattgg
     3301 atttggtaaa gggtctgaac agacaaaaca tcacaccggc cgtttatgcg acgaaatttg
     3361 atcacggcat tcctgaatac gccatgatcg aaccccatct tgtcgatcaa caccggacgc
     3421 tgaaaaaact acgctcattc ctcttttcaa gtcggctcgc tcaaaccaga aaaaacagtg
     3481 ccgccaaact gattgcctgc caccacgccg attacgccga cctcctcatc tgcggcggca
     3541 cacacttggg ctacctgcac catatggcgc aaaaaccgaa cctgctcgac cgtctcgcca
     3601 tacgccgcaa ccgcagcaac tacgccaccg ccaaactgat tatggcgcat tcccatatga
     3661 tgcggcgcga actggtcgga ctgtacggcg ttccccctga aagaatccaa gtcgcccccc
     3721 ccccgcagat acggaacgct tctttccaca acccggagaa actgccgacc tgcgcgccaa
     3781 atacggcttt gccgaccatg aaaccgtttt cctgttccca tcgaccggcc acacgcgcaa
     3841 aggtctggaa ctgcttgccg actttttcga acataccagc ctgcccgtca agctcgccgt
     3901 tgtcggctcc ccgctccccc gccctatgaa aaacgtcgtc gggctgggct tctgcaccga
     3961 tatgcccgaa ctctaccgcg ccgccgactt taccattatg gcctccctgt acgaaccctt
     4021 cgggctggtc ggtgtcgaat ccgtcctatg cggcacacgc gtcgtcctct ccgaaaacat
     4081 ggcatgtaca gagatcatga acgaagaagc cggattcttt ttctcacgcc aaaacccgga
     4141 aaccctggcg caagccgttg cccaagccgt cagccttaaa aaacagggcg gacaccgcct
     4201 gtccgacccg atgcgtgcac tgaactacaa cccgtccctg tcgcaccaca tcgaccgact
     4261 gaccgacatg ctggcatccg tataacaaaa tgccgtctga acgagttttc agacggcatt
     4321 ttcgcaaccg tttcagccat gcggcaaaaa gctaatggac aattttcaaa acgtcgtaca
     4381 gatagttgcg gtaatttccg accaactccc ggtttgggca gtcttccaac cctccgccgc
     4441 acaaaccgcg catcgccata ttcgcacgca tgaagccgtc tttggcttcc aaacctgccg
     4501 cctccatcag cacgccgcct gcaaaggcaa ggtcgagaaa atcctgccgc tgtacgaatc
     4561 cgccggcaat attgctcctg acggcaaact gcgttacata atccggctgc gcgtaatccc
     4621 atttcttcct gacggacacg ccctcaaacg gtacctgatg atcgccgaaa taaccgaaaa
     4681 caaagggttt gccgcgttcg tgcagatagc ggtcgaaact ttccaccgct ttgtccaaat
     4741 cggcaatgcg cccgatgtag tcgttaagcg cggatatggt tttcgcattc aaatcaggcg
     4801 catccaaatc aaacacattg tcggtatctg tccgatacgg cccgtgctcc ttcatggtca
     4861 gcacatatac gaacatcggc tgccgcacgt tttccaaatc gggatggcgt ttttccaaaa
     4921 tcatccgcgc atactgcatc atttcctcac tggaaatatg ccacaggttt ttgcccatcg
     4981 gcgcgggata gcccaaatct tgcggctgga acatcagatt aaagccgaaa tggtcatatg
     5041 ctgccttggc attgtagttg cccttggtaa atggcgagag taccacgcaa aaataaccgt
     5101 gttcgcgcag gttgcggacc aaaccggtct gcaaatgcgg tacgaccgag taaaacacgc
     5161 cgcttgccaa cgcgccgaaa tccgtggacg gaacacccgc caaaaacgca aattcggact
     5221 tccacgttgc gccgccgaaa gtatgcacgc gcaaaggtga ggaaaatacg gtatcttcct
     5281 gccgtccgaa cattttcaaa tcgggaatct tggcggcggc aaagtcgaaa cagtgcggat
     5341 cgagcgtcga ttccatcagg gtaacgacaa tatcgggttt ttcatccgac atgccgcacg
     5401 gccgggtttc agacggcatc tgcctggcaa acgcttcccc gtcgccctcg aacacgggta
     5461 cttggaaaaa caccgcccga caggacatcg gcaggttcaa aaacacgtcg cgcccgtcgt
     5521 ccggcagcga atccagccac gtctttaccg cgcccggatg tttggaaaaa tgccgcatcg
     5581 acacgaacgc cgccgcaaac aaaaccgcgc ctgcccaccg ccacggcaca tccaaagaat
     5641 ccgcaccgct ccagccgaaa accgcatatg ccgccaaagc cagcagcccc gccataccga
     5701 taaccgcttc cttataatgg aacagcgttt cccaattccg ccagtcggca atcagcaaaa
     5761 agtcggaaat caacagcggc tgtttgtaat aatggatttt cagcctgtgg aacagtatca
     5821 gcaccacaaa ccagaccgaa gcgaaattca aggcgcgctg ccactgagcc gtcaacatca
     5881 gcataccgcc cgccaaaaag acaaacagcg cggatgcgaa aaaatacgtc cagcggtagt
     5941 gcgacctgat aatcagcacc aacgccgccg ttacaaacaa aaataggaaa acataggcaa
     6001 ccatcccgtc ccactcctca aaacggcata cctgagccgt tgcgaattat tatcgaaagt
     6061 gtgcattata atgcagcaca ggcgcatcac tgccgatatt tcagtataat tattcgagat
     6121 ggaatcactg aaaatggtga agttacaatg agccctttta gaggaactaa aaagtgaatt
     6181 ataaaatata cgatggtgca aagattatta aaaaagaaaa tttatcaatt ggggattatt
     6241 ctcaaattga tgattttgta ttttttaatt gtggagagaa aagtatttta ggaagttttg
     6301 ttcatatatc atcatttacc agtattattg gtggtggaga attttatatg gatcattttt
     6361 caggattaag cgcagggtgt agaattatta ccggtagcga tgattttatg gggggggggt
     6421 taaccaatcc aacagtccca tctaaa
//
LOCUS       DAXHKX010000111         6415 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHKX010000111 DAXHKX010000000
VERSION     DAXHKX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.111"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            109..1650
                     /locus_tag="ACFMWZ_001546"
     CDS             109..1650
                     /locus_tag="ACFMWZ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC5935489.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAAMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGWQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRACSDKAVSPVMPSEPQSVGEYVLL
                     AFAAAVLGICCLFPLAAVVVKACSAGESWQVLAERETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTAYRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     QTETL"
     gene            1679..2278
                     /locus_tag="ACFMWZ_001547"
     CDS             1679..2278
                     /locus_tag="ACFMWZ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC5935490.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2275..2868
                     /gene="nadD"
                     /locus_tag="ACFMWZ_001548"
     CDS             2275..2868
                     /gene="nadD"
                     /locus_tag="ACFMWZ_001548"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC5935491.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSIRIL
                     SAPMHNASSTEIRRAGVSDGIPPAAARYIREHGLYEK"
     gene            2927..3313
                     /gene="rsfS"
                     /locus_tag="ACFMWZ_001549"
     CDS             2927..3313
                     /gene="rsfS"
                     /locus_tag="ACFMWZ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC5935492.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3367..3837
                     /gene="rlmH"
                     /locus_tag="ACFMWZ_001550"
     CDS             3367..3837
                     /gene="rlmH"
                     /locus_tag="ACFMWZ_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC5935493.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            3912..4178
                     /locus_tag="ACFMWZ_001551"
     CDS             3912..4178
                     /locus_tag="ACFMWZ_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC5935494.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4335..5018
                     /locus_tag="ACFMWZ_001552"
     CDS             4335..5018
                     /locus_tag="ACFMWZ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC5935495.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLNMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5142..5654
                     /gene="coaD"
                     /locus_tag="ACFMWZ_001553"
     CDS             5142..5654
                     /gene="coaD"
                     /locus_tag="ACFMWZ_001553"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC5935496.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5686..6339
                     /locus_tag="ACFMWZ_001554"
     CDS             5686..6339
                     /locus_tag="ACFMWZ_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC5935497.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWRIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1407 a         1701 c         1843 g         1464 t
ORIGIN      
        1 ttcagacggc gggaggaagg cggatacggt atgtagtcgg attgaaatat acggtatgtg
       61 gggcttttgg gtacaatcgc aacatcagtg tttcaagtgg ggaaggcgat ggatggacgg
      121 cgttgggcgg tatggggtgc ttttgccctg ctgccttcgg cttttttggc ggcaatggtc
      181 gttgcgcctt tgtgggcggt ggcggcgtat gacggtttgg cgtggcgcgc ggtgctgtcg
      241 gatgcctata tgctcaaacg tttggcgtgg acggtatttc aggcagcggc aacctgtgtg
      301 ctggtgctgc ctttgggcgt gcctgtcgcg tgggtgctgg cgcggctggc gtttccgggg
      361 cgggctttgg tgctgcgcct gctgatgctg ccgtttgtga tgcccacgtt ggtggcgggc
      421 gtgggcgtgc tggctctgtt cggggcggac ggtctgttgt ggcgcggctg gcaggatacg
      481 ccgtatctgt tgttgtacgg caatgtgttt tttaacctgc ccgtgctggt cagggcggcg
      541 tatcaggggt ttgtgcaagt gcctgcggca cggcttcaga cggcacggac gttgggcgcg
      601 ggggcgtggc ggcggttttg ggacattgaa atgcccgttt tgcgcccgtg gcttgccggc
      661 ggcgtgtgcc ttgtcttttt gtattgtttt tccgggttcg ggctggcatt gctgctgggc
      721 ggcagccgtt atgccacagt cgaagtggaa atttaccagt tggtcatgtt cgaactcgat
      781 atggcggttg cttcggtgct ggtgtggctg gtgttggggg taacggcggc ggcagggttg
      841 ctgtatgcgt ggttcggcag acgcgcgtgt tcggataagg cagtttctcc tgtgatgccg
      901 tctgaaccgc agtcggtcgg ggaatatgtg ctgctggcgt ttgcggcggc ggtgttgggc
      961 atatgctgcc tgttcccgct ggcggcggtc gtggtaaagg cctgttctgc gggagaatct
     1021 tggcaggtgt tggcggaacg cgaaacttgg caggcggtgt ggaatacttt gcgcttctcg
     1081 gcggcggcgg tgtttgcggc ggcggttttg ggtgtggtgt atgcggcggc ggcgcggcgg
     1141 ttggcgtgga tgcgcgggct gatgtttttg ccgtttatgg tatcgccggt ttgtgtttcg
     1201 gcaggcgtgc tgctgcttta tccgcagtgg acggcttcgt tgcccttgct gctggcgatg
     1261 tatgcgctgc tggcgtatcc gtttgtggca aaagatgttt tgtcggcctg ggacgcactg
     1321 ccgccggatt acggcagggc ggcggcgggt ttgggcgcaa acggctttca gacggcatac
     1381 cgcatcacgt tccccctttt gaaaccggcg ttgcggcgcg gtctgacctt ggcagcggca
     1441 acctgcgtgg gcgagtttgc ggcgacattg ttcctgtcgc gtccggaatg gcagacgttg
     1501 acgactttga tttatgccta tttggggcgt gcgggtgagg ataattatgc gcgggcaatg
     1561 gtgttgacat tgctgttgtc ggcatttgcg gtgtgcattt tcctgctgtt ggacaacggc
     1621 gaaggcggaa aacagacgga aacgttataa tgtgaaccct ttttcagagg acggcaaaat
     1681 gagcgagttg gataacatcc ttgcccataa ccggcagttt gtcgagtcgg gcgaatatga
     1741 aaaatacttt accgacaaat accccgaacg cgggctggca gttttgtcct gtatggatgc
     1801 gcggattatc gggctgctgc ccgacgcgtt gggtttgaaa aacggcgatg ccaagctgat
     1861 taaaaatgcc ggcgcgctgg ttacgcaccc gtggggttcg gtgatgcgga gccttttggt
     1921 tgccgtgttt gaactgaagg tcagagagat tatggtcatc gcccatcacg attgcggtat
     1981 gcaggggctg aatgccgaag aattcctcgg gcgcgtccgg gaaagccgga ttcccgaaga
     2041 ccgtatcgaa accctgcgtt atgccggtat cgacctcgac ggctggctga ccggtttcga
     2101 caacgtcgaa gacagcgtgc gccacacggt ggaccttatc cgtaaccatc cgctgatgcc
     2161 gcgccatatc gccgttcacg gactggtcat ccatcccgtt accggcaaac tgacgctggt
     2221 tgtggacggc agtgtttcag acggcatgga cttatcggaa ggaatggaaa catcatgaag
     2281 aaaatcggac tgttcggcgg cactttcgac ccgatacaca acgggcatct ccacatcgcc
     2341 cgcgcctttg ccgacgaaat cggcttggac gcggttgttt tcctgccgac aggcggcccg
     2401 tatcacaaag acgcagcctc cgcttccgcc gccgaccgcc ttgccatggt cgaactggcg
     2461 acggcagaag acgcgcgttt tgccgtcagc gattgcgaca tcgtccgaga aggtgcaacc
     2521 tatacttttg ataccgtcca aatcttccgc cagcagttcc catccgcgca actttggtgg
     2581 ctgatgggca gcgacagcct gatgaagctg catacttgga aaaaatggca gatgctcgtg
     2641 cgcgaaacca atatcgccgt cgccatgcgg cagggcgaca gcctgcacca aaccccgcgc
     2701 gaactgcacg catggctggg caatgccctt caggacggca gcatccgcat cttgtccgcc
     2761 ccgatgcata acgcgtcgtc aacggaaatc cgccgcgccg gtgtttcaga cggcatccca
     2821 cctgccgccg cacgctacat ccgcgaacac ggtttgtatg aaaaataaag tcaaatcagt
     2881 aagaaacggc tataatgccg tctgaaaaca tcccgttagg aaaataatga acgaacaaga
     2941 actgcaagac ctgcaaaaaa tggtcggggt cgccgtcaac gccctcgaag acatcaaagc
     3001 caaagacatt tccgttctcg aaacgcaaga caaaacttcg ctgtttgcca gaatgattat
     3061 cgccagcggc gacagtacgc gccaagtcaa agcactggcc aacaacgttg ccgtcgattt
     3121 gaaagaagcc ggttttgaaa tcctcagcac cgaaggcgac agcggcgaat ggacgttggt
     3181 tgatgcagga gacctcgtcg tccacgtcat gctccctgcc gtgcgcgatt tctacgacat
     3241 cgacaccctt tggggcggcg agaaaccgag tttccacgcc ggaatgcaga agccgtggca
     3301 cgctgcagac tgattcccga tgccgtctga atgttcagac ggcattttcc tgtaagggag
     3361 aaagcattga atattaccgt tttggcagtc ggcaccaaaa tgccgcgctg ggttgatgag
     3421 gccgtcgccg aatacgccaa acgcttcgga cgcgacgtcg cctacgcact caaagaaatc
     3481 aaacccgaaa aacgcggcgc gggcgtgaat gccgcccaag gtatggcggc ggaagaaaaa
     3541 cgcatcctcg aagccatacc gcaaggcgcg ttcctcgtcg tccttgacga acgcggcaaa
     3601 gcaccgacct ccgtcgagct ggcggaacac ctcaaaactt ggcagcaaaa cggcgaacac
     3661 gtctgcttcg tcatcggcgg cgcggacgga atgaccgacc gcctcaaaca acaagcccgc
     3721 atgatgatgc gcctgtccag cctgaccctg ccgcacggga tggtgcgcgt ccttctgacc
     3781 gaacaactct accgcgccgc ctccatcctg cacaaccacc cctaccatcg ggaataagaa
     3841 ggcttttgtc cgcactccca atcggttaaa atccccccgt tatttctgaa cacacagcaa
     3901 aggaatccgc tatggcacgc atggtattct gcgtcaagct caacaaagaa gccgaaggca
     3961 tgaaatttcc gccgcttccc aacgaattgg gcaagcgcat ttttgaaaac gtatcgcaag
     4021 aagcgtgggc ggcgtggacg cgccaccaaa cgatgctgat taacgaaaac cgtttaagcc
     4081 tcgccgatcc gcgcgcgcgc gaatacctgg ctcagcagat ggagcagtat ttcttcggcg
     4141 acggcgcgga tgccgttcag ggatacgttc cgcaataacg gttttccgtt ttgaacacag
     4201 gttgtccgaa actgcttcag acggccttta aaatacgccg gacaacttta tcttcacgca
     4261 accggacaaa ttttgacatt gggcaatgtt aaaatccccc attattccca accgcgcttt
     4321 caggagcaga tgatatgcaa cacgacgttt acgactacac cgcgcatacg gtttctaaaa
     4381 acaccgtcct gcagaaaacc taccgcctgc tcggattttc attcattccg gctgccgcag
     4441 gcgcggcact tgccgccaat gccggtttca atttttacgc cgccttcggt tcgcgctgga
     4501 tagggtttgc cgtcgtgttg gcgtttttct acggtatgat ccacttcatc gagaaaaacc
     4561 gttacagcaa taccggcgtt accctgctga tggtattcac attcggtatg ggcgtattga
     4621 tcggccccgt gctgcaatac gcgctccata ttgccgacgg tgcgaaaatc gtcggcattg
     4681 ccgccgcgat gaccgccgcc gtctttttaa cgatgtccgc cttggcgcgc cgaacccggc
     4741 tcaatatgaa cgcgctcgga cgcttcctga ccgtaggtgc ggtcattctg atggtcgccg
     4801 tggttgccaa tctgtttttg ggtattcccg cactcgccct gaccatttcc gccggttttg
     4861 tcttgttcag ttccttaatg attatgtggc aggtacgcac cgtcatcgac ggcggcgaag
     4921 acagccacat cagcgcggca ctgacactgt ttatctcgct ttacaacatc ttcagcagcc
     4981 tgctgaacat cctgttgtct ttaaacggcg aggattgatg ccggtaaaac gccgtccgac
     5041 tatgccgtct gaaaatgctt cagacggcat tttactttgg gcatacaata cggatgcaca
     5101 cgcaccaaca cacagccgac tatgaagccg ggaggaatca aatgttaccg aatacaccgc
     5161 gccgcgccgt ttatgccggc agtttcgatc cgcccacatt ggggcatctg tggatgatac
     5221 ggcaggcgca atctatgttt gacgaactca tcgtcgccat cggcattaac cccgacaaac
     5281 gcagcaccta taccgtcgct gaaaggcagg atatgttgtg cgatattact aaaatgtttc
     5341 ccaacgtcag aaccgatgta tttgaaaacc gatttctggt gcattacgcc cgtgaggtag
     5401 atgcaggatt catcgtgcgc ggcatccgtt ctgcttcgga ttacgaatac gaacgttcca
     5461 tgcgccatat caacagcgac ctcgcccccg aaatatccac cgtattcctc atgccgccgc
     5521 gcgaaatcgc cgaagtgtcg tccactatgg tcaaaggctt ggtcgggccg gaaggctgga
     5581 cggaaacgat ccaccgctac gtgccgcaag ctgtgtacga aaaaatcctt gccgaacatc
     5641 aacacgaaaa ttgaaccctt ctatttttgc aaggcatata tggatatgtt ggaaattttg
     5701 ttccgccatc aagacttcgt cgccatcaac aaaccgggcg gcatatccgt ccaccaagac
     5761 agcggcgaga ccggactggc ccgaacactc gccatacagt tgggcgtaga gcgtgtgtgg
     5821 ctgctgcacc gattggacaa acagaccagc ggcattttac tgtttgccct caacaaagaa
     5881 agcgcgtccg cccttagcgg acagtttgcc ggcaaaagca tcaaaaaaac ctatttggca
     5941 ctatccgacc gcaaaccgtc caaaaagcag ggttggatta aaggcggaat ggaaaaatcc
     6001 agacgcggaa tgtggaagtt gacgcgcaat acagaaaata tcgccgttac ccgattccac
     6061 agcatcagta tcgccgaaaa actgcggctg ttcatcctgg aaccgcatac gggcaaaacg
     6121 caccaattga gagtggcgat gaaaagtttg ggcagtccga ttctgggcga cagcttatac
     6181 ggcggaacag aatccgaaac catgtttctg tatgcatgga gaatacaatt tgcctaccaa
     6241 aaccgacaaa tcgaaattgt tgcaccctta aaaaacgaat ggcagactga aaatatctct
     6301 catgcgctgg aagaatttta tatggcaaaa gcaggttaat aaatataaat taatttataa
     6361 acaatatgat aaatattttt ataaaatttt ttcaagaaaa cggttgaccg caaat
//
LOCUS       DAXHKX010000112         6388 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHKX010000112 DAXHKX010000000
VERSION     DAXHKX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.112"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            95..1121
                     /gene="hupA"
                     /locus_tag="ACFMWZ_001555"
                     /pseudo
     CDS             95..1121
                     /gene="hupA"
                     /locus_tag="ACFMWZ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
     gene            1152..3578
                     /gene="hupB"
                     /locus_tag="ACFMWZ_001556"
     CDS             1152..3578
                     /gene="hupB"
                     /locus_tag="ACFMWZ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC5935498.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENKGYVFSPTQSFNKYQTYVATGVARSQPDPQEWVNKSTLFKLGY
                     NFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDYRHRQDVSYRRRSGVEYKN
                     ELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWTAD
                     FEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKILTSNQAKITMLIENRSK
                     YKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHAG
                     FSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAKNW
                     ELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDNPNKPTYAPLSDGTALVSSPVW
                     QNQNRTAAWVKGIEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINALSP
                     WSAVYNLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFDL
                     SAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPGR
                     SYNFTIEAKF"
     gene            complement(4185..5819)
                     /gene="groL"
                     /locus_tag="ACFMWZ_001557"
     CDS             complement(4185..5819)
                     /gene="groL"
                     /locus_tag="ACFMWZ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC5935499.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(5912..6202)
                     /locus_tag="ACFMWZ_001558"
     CDS             complement(5912..6202)
                     /locus_tag="ACFMWZ_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC5935500.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRSLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT         1566 a         1880 c         1537 g         1405 t
ORIGIN      
        1 tttttgcacc gacccacaaa accagttgac atcatataac atttaaactg ataatcaaaa
       61 ctattattaa cacatcatca agattgagta tgcgatgaaa tacaaagccc tgtccttact
      121 gccgcttgcc gccgcccttg ccgcctgtgc aggggggggg ggtagccgaa ccgcacatcc
      181 ccgtgtccat ccccaccgcc accccgctgc ccaccggcga ggtaaagtta tcagacgaca
      241 atagcaaaat cgaaaacatc aacaccgccg gcacaggaag cacatccggt atttccattc
      301 agcagcggga agtagaaaaa gaacctttcc ccggctacaa aaccaaagaa acatcattca
      361 tcttccaaac acccggcggc gcgcaatacg ccctttcatc ctacgccgac cccataaccg
      421 tcagctactc ctcccccgat ttcaaaattc ccgaccgcca cgcagggcag cggcttgccg
      481 acggcagccg catctttatc tgttgcagcg aatccggggc tacttcgtat gcggaaatca
      541 ccaaacaaga ttatatgaaa ttcggggcgt ggataggtcc caacggcgaa atcgacctct
      601 tcgccggcgg cttccccgtc ggcaaaacgc cgaaaccggc atactcctgg agcgacgaca
      661 cgcccgaaac ccaaggcaaa ggcaaaatca cttatcaggt ttggggcatc cgcgtcaaag
      721 acgggcaatt tgttacctcc tcttatacgc cgcccaaagg cagctctttt accggctaca
      781 ccaacacccc cgtcctttcc tttatcaccg ccaatttcaa cagcaacaaa ctggcaggcg
      841 aaatccgcgg caacagcgat tacggaccga gtgtgaaaat tgaaaacgca acgattgacg
      901 gactatcctt ttccggcgat gccacgtccg gcggcaaaaa cggcaaattg gaaggcaagt
      961 tttttggcaa gttcaacagc acccgcagca gcgacaccgg cataggcggc aaaatcactt
     1021 ttaaagacga ccgctccctc gataccgtat tcggcggcgt gatttatgag aaaaaacttg
     1081 atgacaaaac cagtcaagat accaaccatc tcaaaaaata ataacccatc cgcttttaaa
     1141 aaggttttct tatgcccatc ccctttaaac ccgtattggc tgccgccgcc atcgcccaag
     1201 cgtttcccgc ctttgcggca gaccccgcgc cgcagtccgc ccaaacgctg aacgaaatca
     1261 ccgttaccgg cacgcacaaa acccaaaaac tcggcgaaga aaaaatccgc cgcaaaactt
     1321 tagacaagct cttggtcaac gacgaacacg acctggtgcg ctacgacccc ggcatttccg
     1381 tcgtcgaagg cggcagggcg ggttctaacg gctttaccat acgcggcgtg gacaaagacc
     1441 gcgtcgccat caacgttgac gggctggcgc aggcggaaag ccgctcttcc gaagccttcc
     1501 aagagttgtt cggcgcgtac ggcaacttca acgccaaccg caacacctcc gagccggaaa
     1561 acttttccga agtaaccatc accaaaggcg cggactcgct taaatccggc agcggcgcat
     1621 tgggcggcgc agtcaattac caaaccaaat ccgcaagcga ttatgtttcc gaagacaagc
     1681 cctaccattt ggggataaag ggcggcagcg tcggcaaaaa cagccaaaaa ttcagcagca
     1741 tcaccgccgc cggcaggctc tttggtttgg atgccttatt ggtttatacc cgccgcttcg
     1801 gcaaagaaac caaaaaccgc tcgaccgagg gcgatgtcaa aattgaaaac aagggatatg
     1861 ttttcagccc aacccaatca ttcaacaagt accagaccta cgtagccaca ggggttgcgc
     1921 gctcccaacc cgacccgcaa gaatgggtaa acaaaagcac cttgttcaag ctgggctaca
     1981 atttcaacga ccgcaaccgc atcggctgga tttttgaaga ctcccgcacc gaccgtttta
     2041 ccaacgagct gtctaatttg tggacgggta caatttactc tcccgcaacg ggcgactacc
     2101 gccaccgcca agacgtgagc taccgccgcc gctccggcgt cgaatacaaa aacgaattgg
     2161 aacacggccc gtgggacagc ctcaagctgc gctacgacaa gcagcgcatc gatatgaaca
     2221 cttggacttg ggacatcccg aaaaattacg atttgcgcgg tatcaacagt gaagtttacc
     2281 attcgttccg gcatatccgc caaaacaccg cgcaatggac tgccgatttt gaaaaacaac
     2341 tcgacttttc caaagccgtt tgggcggcgc aatacggctt gggcggcggc agaggggaca
     2401 atgccaactc ggattacagc tatttcgtaa aactttacga ccccaaaatc ctcacttcca
     2461 accaagccaa aatcacaatg ctgatcgaaa accggtcgaa atataaattt gcctattgga
     2521 acaatgtgtt tcacttgggc ggcaacgacc gcttccgcct gaatgcgggc atacgctacg
     2581 acaaaaacag cagcagcgcg aaagacgatc cgaaatacac caccgccatc cgggggcaga
     2641 ttccccattt gggttcggaa cgcgcgcacg cgggcttcag ctacggcacg gggttcgact
     2701 ggcggtttac caagcatctg cacttgttgg caaaatacag caccggcttc cgcgcaccga
     2761 cttcggacga aacctggctg ctgttcccac accccgattt ctacctgaaa gccaacccaa
     2821 acctgaaagc cgaaaaagcc aaaaactggg aattgggtct ggcgggcagc ggcaaagcgg
     2881 gcaacttcaa gctctcgggc ttcaaaacca aataccgcga ctttatcgaa ttgacgtata
     2941 tgggcgtttc gtcagacaac cctaacaagc ccacctacgc cccgctttca gacggtacgg
     3001 cattggtcag ctcgcccgtt tggcaaaacc aaaaccgcac cgccgcctgg gtgaaaggca
     3061 tagagtttaa cggcacttgg aacctcgaca gcatcggttt gccgcaaggc acgcacgcag
     3121 gtgtcaatgt cagctacatc aaaggcaagg ccaaacaaac caacggacag gaaaccccta
     3181 ttaacgccct ttcgccatgg agtgcggttt acaatctggg ctacgacgct ccttccaaac
     3241 gttggggcat caacgcctac gccacgcgca ccgccgccaa aaagccgtcc gacaccgtcc
     3301 acagcaacga cgacttgaac aacccgtggc cttatgccaa acacagcaag gcctatacgc
     3361 tgttcgacct ttccgcctac ctcaacatcg gcaaacaggt tacgctccgc gccgccgcgt
     3421 acaacattac caacaagcag tactacactt gggaatcttt acgcagcatc cgcgagttcg
     3481 gcacggtcaa ccgcgttgac aacaaaaccc acgccggcat ccaacgcttt acctcgccgg
     3541 gcaggagcta caatttcacc atcgaagcga agttctaagc tcctgatgtg ccaatgccgt
     3601 ctgaaacccg atgatttggg tttcagacgg cattttttac ggtcgcaccg ccgccccctt
     3661 tcaggcttgt accaagccca atcccgcaac tctccgtcat tcccgcaact ctccgtcatt
     3721 cccgcgactc tccgtcattc ccacggaagt gggaatctag aaacgcaaag ctgcaagaat
     3781 ttatcggaaa tgactgaaac tcaacgaacc tggattcccg cctgcgcggg aatgacgaag
     3841 ctatccgcac agaaacctgc accacgtcat tcccacgaaa gtgggaatcc agaacgtaaa
     3901 atctgaagaa accgttttat ccgatacgtt tccgcaccga cagacctaga ttcccgcttt
     3961 cgcgggaatg acggcggaaa ggttgctgtt tttccgataa attcctgccg ctcttcgttt
     4021 ttgggatggc gggaaataaa acaaaagcgc gcgtatcaaa aaataacaat gcaaagaacg
     4081 gtgttggcaa ttttaaggtt tgcgccttag ccgcacttat tcgcaaaaaa accgcacggc
     4141 gttgaccgtg cggcttgttg tctgaaggct tcagacggca ttgcttacat catgccgccc
     4201 ataccaccca tgccgcccat atcaggcata gccggtttgt cttcagggat ttcagcaatc
     4261 atgcagtctg tggtcagcat caggccggcg atagacgcgg cgtgttgcag cgcggaacgg
     4321 gttactttgg cggggtcgag tacgcccatt tcgatcatgt cgccgtattc gccgctgcca
     4381 gcgttgtaac catagttgcc tttgccttcc aacactttgt tcacaaccac gctgggttcg
     4441 ccgcctgcgt tggcaacgat ttggcgcagc ggagactcaa cggcgcgcaa gacgatttgt
     4501 acgcctgcgt cttggtctgc attgccggtg tgcaggtttt ccagagcggc acgggcgcgc
     4561 aacagggcta cgccgccgcc tgcaaccacg ccttcttcaa cggctgcgcg ggtagcgtgc
     4621 agcgcgtctt ccacgcggtc ttttttctct ttcatttcca cttcggtcgc ggcaccgact
     4681 ttgattactg ccacgccgcc tgccagtttg gcaacgcgct cttgcagttt ttctttgtcg
     4741 taatcgctgg ttgcggtttc gatttgttgg cggatttcgg caacacgcgc ttcgatttgg
     4801 gctgcgtcgc cgaagccgtc gatgatggtg gtgttttctt taccgatttc gatgcgtttg
     4861 gcctgaccca agtcgtccaa agtcgctttt tccaaagaca ggccgacttc ttcggaaatc
     4921 actgtgccgc cggtcaggat agcgatgtct tgcagcatcg ctttgcggcg gtcgccgaag
     4981 cccggagctt taacggcaac ggttttcaga atgccgcgga tgttgttcac caccaaagtc
     5041 gccaaggctt cgccttctac gtcttcagcg ataatcaaca gcggacggct ggctttggcc
     5101 acttgttcca aaacaggcag caggtcgcgg atattgctga tttttttgtc gaacagcaat
     5161 acaaacggat tgtccaagcc ggcgatttgt ttttccgcat cgttgatgaa gtaaggagac
     5221 aggtagccgc ggtcgaattg cataccttca accacgtcca gctcgttttc caaagatttg
     5281 ccgtcttcaa cggtaatcac gccttctttg ccgacttttt ccatcgcttc ggcgataatc
     5341 gcgccgactt gttcgtcgga gttggcggaa atagagccga cttgggcgat ttctttagaa
     5401 gtatcgcaag gtttggcgat gtttttcagc tcgtcaacca aagcggcaac ggctttgtcg
     5461 ataccgcgtt tcaggtcggt cggattcata ccggcggtaa cgtatttcat accttcggca
     5521 acgatggatt gcgccaatac ggtggcggta gtcgtaccgt cgcccgccac gtcgttggtt
     5581 ttggacgcga cttctttcac catttgcgcg cccatatttt caaacttgtc tttcaattcg
     5641 atttctttgg cgacggttac gccgtctttg gtgatgtgcg ggccgccgaa tgcgcggtca
     5701 accaccacgt tgcggccttt gggacccaag gttacgcgta cggcgtttgc cagaatgttc
     5761 acgccgttta ccattttttg acggacttca ttgccgaact gtacgtcttt tgctgccatt
     5821 tcaattctcc aaaaatcatt aaaactgtct gataaaaccg tttatgccgt ctgaaggcgg
     5881 tttgccgttt cagacggcat cgtgtccgta tttatttttc aacgatgccg aagatatctt
     5941 cttcgcgcat taccaacagc tcttcgccgt cggcttttac ggtttggccg ctgtatttgc
     6001 cgaaaatgat tttgtcgccg actttgacat ccagcgaacg gcgcgcgccg tctttgccga
     6061 ttttgcccgc gcccacggcg atgacttcgc ccatatcggg tttttcggcg gccgcacccg
     6121 gcaaaacgat gcccgatgcg gttttctctt cagcttccaa gcgtttgacg acgacgcggt
     6181 cgtgtaaagg acggatggtc atatctatgc tccgataaaa tggtttgaaa acaatcatct
     6241 gcccgaacgg ttcggacaga ttaaggatga aaaccggaca gccgtcaggc ggctgcccgt
     6301 ataagtcggc aaaattaggg tgtgtgcggg caaattcaag tgaggcggaa aaaatttatt
     6361 tccgccgttt tttatagtgg actaaatt
//
LOCUS       DAXHKX010000113         6307 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHKX010000113 DAXHKX010000000
VERSION     DAXHKX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.113"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(540..2576)
                     /locus_tag="ACFMWZ_001559"
     CDS             complement(540..2576)
                     /locus_tag="ACFMWZ_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC5935501.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAAYNELMPEITAFFTEIGQ
                     DIALYNRFKTIKNSPEFATLSTAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2664..3536)
                     /locus_tag="ACFMWZ_001560"
     CDS             complement(2664..3536)
                     /locus_tag="ACFMWZ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC5935502.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPADSETL"
     gene            3806..6196
                     /gene="gyrB"
                     /locus_tag="ACFMWZ_001561"
     CDS             3806..6196
                     /gene="gyrB"
                     /locus_tag="ACFMWZ_001561"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC5935503.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINNYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRIIDELVLHAMLHASPVDLSTAESA
                     DRSVAELNGLLDEKEVALERIEGHEGHQFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGKGAKLYKGDNEYDIDSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
BASE COUNT         1465 a         1658 c         1777 g         1407 t
ORIGIN      
        1 ccttgccctg atttttgtta atccactata aaatgccgtc tgaagcgttg tttgaccgtt
       61 tcagacggca tttttatcaa attcaaaaat attccgtcat tcccgcgaaa gcgggaatct
      121 agaacgtaaa atctaaagaa accgttttat ccgataagtt tccgcaccga cagacctgga
      181 ttcccgcctg cgcgggaatg acgggatttc tgtttttgat ttttttgttt ttggggaatg
      241 acaggatttg agattgcggg catttatcag gtaaaacgga aattaaacgt tgcgaaaatt
      301 tatccgaaat cacagcaact tttccgcgtc attcccacga aagtgggaat ctaggactta
      361 ggaccggaga aaccttttta cccgataagt ttccgtgccg accggtctgg attcccgcct
      421 acgcgggaat gacgggattt tggcttactg tttttgtgaa aatggcgaaa ccgcgcaaat
      481 gctgatgccg tctgaaacac tgtttgattg tttcagacgg cattttttgt tcacatcgat
      541 caagccgcgt tgtcgaaacc gctgtggcgc aagagtgcgt ctatgcgcgg ttcgcgtccg
      601 cggaaggctt tgaaggattc tgccgcgctg cgcgatccgc cgacggcgag gatttcctgc
      661 caaaagcgtt tgcctgtggc ggcgacatcg tcgctttctt caaaggcggc gtatgcgtcc
      721 gcgctcaata cttccgccca cgcgtagctg taatagcctg cggaatagcc gcctgcgaag
      781 atgtggccga agctcaaggc gaagcggttg tattcgggcg gttggatgac ggcgacttct
      841 ttgcgcacgc tgtctaaaac ctgttgccag tttttcagac ggccttcgtc gtcttcgctg
      901 taaatcatca tatcaaagag ggcgaactcc atttgccgga cgaggaacat gccgcgttgg
      961 aagtttttgg cggcgagcat tttgtcgaag agttctttcg gcaggggaac gccggtttct
     1021 tcgtgggcgg acatttgtgc caagacgtcg tattcccaga cgaaattttc cataaactgg
     1081 ctgggcagtt cgactgcgtc ccattctacg ccgttgatgc cggatacgcc cagttcgtcc
     1141 acttgggtaa gcaggtggtg cagcccgtgt ccggtttcgt ggaagaggat gaggatttcg
     1201 tcgtggctca aacgcgcttc tctgccgccg acgggcgggg cgaagttgca gacgaggtag
     1261 gcggtgggca gttgcagcgt gccgtctgaa aaacggcggc gacctttgta gtcgttcatc
     1321 cacgcgccgc cgcgtttgcc ttcgcgtgcg tacaaatcca tataaacgcc gcctatggtt
     1381 tcgccgtttt gttgcaattc aaaatagcgc acgtctttgt gccagacggg gacggttttt
     1441 tcggtaaatc cgatgccgta gagttttttg atttgggcga acagtccgtt taatactttg
     1501 ccgacgggga agtatttttt gacttcggtt tcgctgaatg cgtatttggc ttcgcgcagt
     1561 ttttcgccgg cgtagcccaa gtcccacggt tgcaaatcgg cgagaccgag gctttcgcgg
     1621 gcgaaggctt tgacttcggc gaggtctttt tcggcgtagg gtttggcgcg gcgggcaagg
     1681 tcgtgcagga agtttaaaac ttgttcgggg gtgtccgcca ttttggttgc cagcgacaat
     1741 tcggcgtagt ttttgaagcc gagcagtttg gcggtttgca gggcgtttgc gagcgtgcgg
     1801 tcgatgttgg cggtgttgtc gaatttgccg ccgtctgaca gttcgctggc gcgggtaacg
     1861 taggcgcggt agatttgttc gcgcagttcg cggttgtcgg cgtattggat gacggcgagg
     1921 tagtgcggaa tctgcaaacc gattttgtag cctgttttgc cttcgctttg cgcggcagcg
     1981 gcaaacatgg cgagcgcgtc ttcgggaatg ccggcaagcg gtgcggcatc gtcaaagtaa
     2041 atgccgaacg cgtcggtcgc gtctaggacg ttttgggaga atttggcgga aagttgcgcg
     2101 ccttcggttt gcagttttgc cagttctgcc tgctgttcgg gcggcagttc cgcgccgctg
     2161 aggacgaaat cgcgcaggtc gtggttgagc ttggtttttt gtgcggtgga aagcgttgca
     2221 aattcggggg agtttttgat ggttttgaag cggttgtata aggcaatatc ttgtccgatt
     2281 tcggtaaaga aggcggtgat ttcgggcatt aattcattgt aggcggcgcg cagttcgggc
     2341 gtgtcggcga cggagttgag gtgcgacacc acgccccaaa tcctgccgac gcgttcggtg
     2401 atgccggtca ggggttcgac agtgtttgcc cagccggtgt gcgtttgggc tttgatggcg
     2461 gcgatttgtt cgcgcgcttc ggcgatggcg gtttgcaggg cgggtttgat gtcttcggtt
     2521 ttgatttgat caaaacgggg ttcttcgccc aaatggagca gtgcgttgtc agtcataaga
     2581 tgggtttcct ttgcgtgttg tgcttcagac agcatatcgg caatatgccg tctgaagccg
     2641 gaaatggggt cggtcgggga aagttaaagt gtttcggagt cggcgggatg atttaacagg
     2701 cggtattgta cgcgcaaaaa ttcgtccata accgcctgtt ggatttccag ccgctttgaa
     2761 acgggggagg cgaagcggac gatgatgcgg tatgccttgt cgtcatacgg tacgcgggta
     2821 acgcgcggtc tggcggcggg cgtgataaac agtttttccg cctgcacgtt ttccaaatgc
     2881 cgctggatgg cggggatgta gggcgcgcac aagggctcga gtacggcttt cagacggcat
     2941 acggcctcat ccgaatccaa atggatgggg acggggattt cgaccgtatg gatgacgtag
     3001 tcgcccaaaa tattgtcgcg gcgcacgggg tggcttaaca acaggctgtt ggggaaagaa
     3061 acggtggttc ccgcaagctg tccgaccaag gggttcgggc cgacctgcat catcagcgtg
     3121 ttcaacaggt tgatgtcgac cacgcgcccg cgcaggccgt tgatttcgat atagtcgccg
     3181 accgagtatt gctgggtggc agaccttaaa atactgcccg acagacacat aatcagttcc
     3241 ttcgtcgcca cgacgaccgc cgccgccacc gcaaacatcg acaaagccag cgtttgaatt
     3301 tgcgccgacc agataaatgc cagcgaaaac agcaccaaaa gcagcgttat attgcggctg
     3361 gcaaccaaaa accgccgctt gctttcgatg ccgaaatccg gatgccgttt gaagtggata
     3421 ttcaacagaa gggcgcgcgc cagcagcaaa gccgcaaccg ccgccacgga ttcgaccgcc
     3481 tccgcacgta tcgggacggc accgagccaa gtgtccaaca tattccatat ttccatttcc
     3541 gcgcccctgt ccgaaaagta tagggtgtag attttagtgg caaaaaaacg cttttgccac
     3601 tttacgggac aatccccgac ctgatgccgg aggcaaacgt accgtttcat cttttcccgg
     3661 cggcacactg atgcggataa cgctgaatta ggcggcaaaa tcggctataa tacacaatta
     3721 tatatgtcgt cccgcccgct ttttctacaa ggtgcggact tgttttggcg gcatcgcaaa
     3781 atcttttcaa aatccggcaa aaaatatgac tgaacaaaaa cacgaagaat acggcgccga
     3841 cagcattcag gtgctcgaag gcttggaagc ggtacgcaaa cgccccggca tgtacatcgg
     3901 cgacacgcag gacggcagcg gtctgcacca catggtgttc gaagtattgg acaacgccat
     3961 tgacgaagca ctcgccggac attgcgacaa aatcacggta acgatacacg ccgaccattc
     4021 cgtcagcgtc gccgacaacg ggcgcggtat gcccaccggc atccacccga aagaaggacg
     4081 ctccgccgcc gaagtcatca tgaccgtatt gcacgcgggc ggtaaattcg acaacaacag
     4141 ctacaaaatc tccggcggcc tgcacggcgt gggcgtgtcc gtcgtcaacg cgctgtccga
     4201 ctgggtaacg ctgaccatct accgcgacgg caaagaacac ttcgtccgct tcgtgcgcgg
     4261 cgaaaccgaa gagccgctga acgttgtcgg cgattccgat aaaaaaggca cgaccgtgcg
     4321 cttcctcgcc agtacggaaa ccttcggcaa cgtcgaatac agcttcgaca tccttgccaa
     4381 acgcatccgc gaactttcgt tcctgaacaa cggcgtggac atcgaattga ccgacgagcg
     4441 cgacggcaaa cacgaaagct tcgccctttc cggcggcgtg gctggattcg tgcaatacat
     4501 gaaccgcaaa aaaacgccct tgcacgaaaa aatcttctac gcgttcggcg agaaagacgg
     4561 catgagcgtc gaatgcgcga tgcaatggaa cgacagctat caggaaagcg tgcaatgttt
     4621 taccaacaac attccgcaac gtgacggcgg tacgcacttg accgcgctgc gccaagtgat
     4681 gacccgcacc atcaacaact atatcgaagc caacgaagtg gccaaaaaag ccaaagtgga
     4741 aaccgccggc gacgatatgc gtgaaggttt gacctgcgtg ttgtccgtca aactgcccga
     4801 ccccaaattt tcgtcccaaa ccaaagacaa actggtttcc ggcgaaatcg gtcccgtcgt
     4861 caacgaagtc atcagccaag ccctgaccga cttcctcgaa gaaaaccctg ccgaagccaa
     4921 aatcatcacc ggcaaaatcg tcgatgccgc ccgcgcgcgc gaagccgccc gcaaagcccg
     4981 cgaaatcacc cgccgcaaag gcgtaatgga cggcttgggg ctacccggca aactcgccga
     5041 ctgccaagaa aaagaccccg cattgtcaga actttatttg gtcgagggcg attctgcagg
     5101 cggttccgcc atgcaggggc gcgaccgcaa attccaagcg attttgccgc ttaagggcaa
     5161 gattttgaac gtcgaaaaag cgcgctttga aaaaatgctc gccagtcagg aagtagccgc
     5221 tttgattacc gcgttgggcg cgggcatcgg caaagaagaa ttcaatgccg agaagctgcg
     5281 ttaccaccgc atcattatca tgaccgatgc cgatgtggac ggtgcgcata tccgcacctt
     5341 gcttttgacc ttcttctacc gccaaatgcc cgagctggtc gaacgcggct acatctacat
     5401 tgcccagccg cctttataca aagccaaata cggcaaacaa gaacgatatc tcaaagacga
     5461 attggaaaaa gaccaatggc tgctcggttt agccttggaa aaagccaaaa tcgtttcaga
     5521 cagccgcacc atcgaaggcg aagagcttgc caataccgcc aaacaattcc tgttggccaa
     5581 aaccgtcatc gagcaagaaa gccgcatcat tgacgaactc gtcctgcatg ccatgctaca
     5641 tgcttcgccg gtcgatctca gcacagcaga aagcgcagac cgctccgttg ccgaattgaa
     5701 cggcctcttg gacgaaaaag aagtcgcgct cgaacgtatc gaaggccacg aaggacacca
     5761 gttcatcaaa atcacacgca agctgcacgg caatgtggtg gtgagctacc tcgagcctaa
     5821 gttcctcaac agcaaagcct atcaaaccct tacccaaacc gccgccgcac ttaaaggcat
     5881 ggtcggcaaa ggggcgaagc tctacaaagg cgacaacgaa tacgacatcg acagctttga
     5941 aaccgctttg gacatcttga tgagcgttgc ccaaaaaggt atgtccatcc aacgatacaa
     6001 aggcttgggc gagatgaacc ccgaacagct gtgggaaacc acgatggatc ccgccgtgcg
     6061 ccgcctgttg aaagtgcgca tcgaagatgc cattgccgcc gacgaagtat tcgttaccct
     6121 gatgggcgac gaggtcgaac cgcgccgcgc ctttatcgaa aacaacgcgc tgattgccca
     6181 aaatatcgac gcataagtgc cgttttaaaa aaggagacgg gcatcgtgcc gcgtctcctt
     6241 ttggtttgtc aaacggaagc catgccgtct gaaaccgcct ttcggagcaa aaccatgatc
     6301 agcattt
//
LOCUS       DAXHKX010000114         6307 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHKX010000114 DAXHKX010000000
VERSION     DAXHKX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.114"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(712..1296)
                     /gene="rnhB"
                     /locus_tag="ACFMWZ_001562"
     CDS             complement(712..1296)
                     /gene="rnhB"
                     /locus_tag="ACFMWZ_001562"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC5935504.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1364..3259)
                     /gene="mnmG"
                     /locus_tag="ACFMWZ_001563"
     CDS             complement(1364..3259)
                     /gene="mnmG"
                     /locus_tag="ACFMWZ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC5935505.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3584..5011)
                     /locus_tag="ACFMWZ_001564"
     CDS             complement(3584..5011)
                     /locus_tag="ACFMWZ_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC5935506.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5171..6160
                     /gene="pdxA"
                     /locus_tag="ACFMWZ_001565"
     CDS             5171..6160
                     /gene="pdxA"
                     /locus_tag="ACFMWZ_001565"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC5935507.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
BASE COUNT         1332 a         1649 c         1822 g         1504 t
ORIGIN      
        1 tcattcccgc aaaaacaaaa aaatcaaaaa cacaaaactg aaatatcgtc attcccgcgt
       61 aggcgggaat ctaggtctgt cggtacggaa acttatcggg aaaaacggtt tttccaaccc
      121 tgagactccg gattcctgtt ttcgcgggaa tccggttttt tgagtttcag tcatttttga
      181 taaattcttg cagctttgag tttctagatt cccgcttttg cgggaatggc ggtttggaag
      241 ttacctgaaa ttcaaaaaaa acggaaaccg gacggattgg attcccgcct gcgcgggaat
      301 gacggatttt aggtttcttt tttgagtttc agtcattttt gataaattct tgcagctttg
      361 agtttctaga ttcccgcttt cgcgggaatg acggtttgga agttacctga aattcaaaaa
      421 aaaaacggaa accggacgga ttggattccc gcctgcgcgg gaatgacgga ttttaggttt
      481 ttttttgatt ttctattttt cgcaggaatg acggtttgga agttacctga aattcaaaaa
      541 aaaaaacgga aaccggacgg attggattcc cgcctgcgcg ggaatgacgg attttaggtt
      601 ttttttttga ttttctattt ttcgcgggaa tgacggtttg ggttctttct ctttggagtt
      661 gcgatgccgg aaatgccgtc tgaaggcttc agacggcatt tttgtgccgg tttaaaacaa
      721 ggcctgctgc gcgagcaggt ttctgacggg ggcgaagtcg cggcggtgtt cgggcagcac
      781 gccgtattgt ttgagggctt ccagatgttg cttcgtgccg taacctttgt gtttgtcgaa
      841 accgtattgg ggatggcgtt gcgccagtgc gtacatttcc gcatcgcgtg cggtctttgc
      901 caaaacggat gcggcggaga tttcgatgat tttgctgtcg cctttgacga cggcttcggc
      961 agggatgttc aaatgttcgg gaatgcggtt gccgtcgatg aatattcttt cgggacgcac
     1021 agccaaaccg tcaacggcgc gtttcatcgc gagcatggtg gcgtgcagga tgttgaggct
     1081 ggcgatttct tcgggcgagg cggcggcaac gtgccacgca accgcctgat tttttatcat
     1141 ttcggcaagc gcgtcgcgtt ttttctcgct gagttttttg gagtcggtca gtccgggcag
     1201 gtcgaatatt tccggaagga tgacggcggc ggcaaacacg ctgccgacta aaggcccgcg
     1261 ccctgcctcg tccacgccgg cggtcagtat gtgcatgatg tttcctgtcg ggatggcgcg
     1321 atgccgtctg aacggtgttt cagacggcat cgcgccgatg tgtttatttc gcgtctttaa
     1381 acccgcgctt caaatgcacc atcagcaatg ccactgccgc aggggttacg ccggaaatgc
     1441 ggcttgcctg cccgacggtt tcgggtttgt gctggttgag cttttgctgc acttctgccg
     1501 acaaaccttt aactttgctg taatcgatat cctcgggcag ctttaaggtt tcgatgtcgc
     1561 ggcggctgtc gatttcttcg ttttggcggt cgatatagcc ttggtatttg acttggattt
     1621 cgacttgttc gaccacttcg gcggagaggc gttcagacgg catcgcgcct tcgagcgtca
     1681 tcagcgcggc gtagtcgagg ttcgggcggc gcaggaggtc gtgcaggttg gcttcgcggc
     1741 tgagcttttg cccgaatacg cggatttgtt cgtcttcggc gagtttttgc ggcgtgtacc
     1801 acgttgtttt caaacgttgg atttcgcgtt cgatggcttc gcgtttttcg ttgaacatgc
     1861 gccattgcgc ttcacccacc aagccgattt tgtagccgtc ttcggtcagg cgcatatcgg
     1921 cgttgtcttc cctgagttgc aggcggtatt cggcacggct ggtgaacatt cggtagggtt
     1981 cgttcacgcc tttggtgatg aggtcgtcca ccaatacgcc gagataggct tgttcgcggc
     2041 gcagcaggag cgggtcttgt tcgcggacgt attgcacggc gttcgcgcct gccagcagtc
     2101 cttgcgcggc ggcttcttca taacctgtcg taccgttgat ttgcccggcg aaaaacaatc
     2161 cggcaatggt tttggtttcg aggcttgctt tgaggttgcg cggatcgaag tagtcgtatt
     2221 cgatggcgta gccggggcgc aggatatggg cgttttccaa acctttcata ctgcggacga
     2281 gcgcgatttg gatgtcgaac ggcaggctgg tggagatacc gttggggtag tattcgtgcg
     2341 tagtcaggcc ttcgggttct aggaaaatct ggtggctgtc tttgtcggcg aagcggttga
     2401 ttttgtcttc gatagacgga caataacgcg ggcccacgcc ttcgattttg ccggtaaaca
     2461 tcgggctgcg gtcgaagcct gagcggatga tgtcgtgggt ttgcgtgttg gtatgcgtaa
     2521 tccagcagga cacttggcgc gggtgcattt cggcgttgcc gcgtacggac atgacgggaa
     2581 cgggcgtgtc gccgggctgt tcggtcagtt gggagaagtc aatcgtgcgt ccgtcgatgc
     2641 gcggcggcgt gccggttttc agacggcctt gcggcaggtt caattcgcgc aaacgtccgc
     2701 ccagcgattt ggcggcgggg tcgccggcgc gtccgccctc gtagttttcc aaaccgatgt
     2761 ggattttgcc ggacaaaaac gtgcctgcgg tcaacaccac ggcgcgtgct ttaaactcca
     2821 cgcccatcgc ggtgattacg cctgaaatgc ggtcgccatc gagcgttacg tcttcgacgg
     2881 cttgttggaa aaggtcgagg ttttcttggt tttccaacat ttcgcggatg gcggctttat
     2941 ataggatacg atccgcctgc gcgcgcgtgg cccgtactgc cgcgcctttg ctggcgttca
     3001 gacggcggaa ctggatgccg gatttgtcgg ttgccaacgc catcgcgccg ccgagcgcgt
     3061 cgagttcgcg caccaaatgc cctttgccga tgccgccgat ggaagggttg cacgacattt
     3121 gtccgagcgt ttcaatattg tgtgtgagca aaagcgtctg cgcgcccata cgggcggcgg
     3181 cgagcgcggc ttctgtgccg gcgtgtccgc cgccgacgac gataacgtcg taggttttgg
     3241 ggtaaatcat gtgggtcatg tgtgtattgc ctgacggtgt ttcagacggc atttatagtg
     3301 gattaacaaa aaccagtaca gcgttgcctc gccttagctc aaagagaacg attctctaag
     3361 gtgctgaagt accaagtgaa tcggttctgt actatctgta ctgtctgcgg cttcgccgcc
     3421 ttgtcccgat ttttgttaaa ccactatatt caatatgccg tctgaaaaac gaaatggatt
     3481 caaaagtaaa gggttgggat tgtacgcttg ttcgccctgt ttttacagtg tgcggaaagg
     3541 gaaaagccgc ttcgcgggga agcggctccg gtaagggcgg gatttaccaa acgtcggatt
     3601 tgatacggcg tttcaggccc ggatgttcgg aaagtttgaa ctcggggtct ttgcccattt
     3661 tcagcttggc ggtgtaatcg cgcagcagca taaacgccag tggcgagagc agcaggatgg
     3721 cgacaaggtt gatccacgcc ataatgccca tcgccatatc cgccatatcc cagaccaaag
     3781 gcacattggc aaccgcgccg aaatagaccc acgccaaaac cagcatacgg aaaacggcgg
     3841 taatcagcca atggcttttg atgaattgga cgttggactc ggcataggca tagttgccga
     3901 taacggtgga aaaggcaaac ataaacagga tgacggcgag gaagcccgcg ccccattgcc
     3961 ccacttggct gacaatcgcc gcctgcgtca gcgccgcacc gctcaaatcg ccgtaaggct
     4021 gttggtaaat caagatgatg aaggcggtgc aagaacaaac gatgatggta tcgacaaaca
     4081 cgcccagcat ttgaatcata ccttgcgaaa cagggtgttt cacttcggcg gcggcggcgg
     4141 cgttcggcgc ggaacccata cccgcctcgt tggaatacag gccgcgtttg atgcccatca
     4201 tcatcgtttg cgaaatcaga ccgccgagta agccgcctgc tgccgcgtcg aatttgaacg
     4261 cgcccgaaaa aatctgaccg aacacgtccg gaatcatcgg aatattggtc aaaatgatga
     4321 aaagcgcgat aaagaggtac aaaaccgcca tcagggggac gacgatttcc gccgctttag
     4381 atatgcgcct gatgccgccg aagataatcg gcgcggttaa aatcaccagg gcgacgccga
     4441 cataatgagg ctcccaaccc cacgccgctt tgacggtatc ggcaatggta ttggtctgaa
     4501 ccgcttcaaa cacaaagccg aaacagaaaa tcaggctcag ggcgaacaac acgcccagcc
     4561 atttctgccc cagcccttga gtgatgtagt aggcagggcc gccccggaaa tggtggttgt
     4621 cgtagtcgcg gactttaaag agctgcgcca gcgaagattc gacaaacgcc gaactcatac
     4681 cgattaaggc ggttacccac atccaaaaca ccgcgcccgg tccgccgact ttgatggcga
     4741 tggccacgcc cgcgatattg cccacgccca cgcggctggc aaggccggtt acaaatgcct
     4801 gaaacggcgt gatgccgtga gggtcgtccc cctgtttgcg gccgccgagc atttctttga
     4861 tgctgcgccc gaacaggcgg aattggacaa agcccgtggt tacggtgaag aaaagccccg
     4921 tacccaaaag catataaacc aagtatgacc acatcggatc gttgatggcg ccgacccagc
     4981 cgtgcagcca ttcggtaaag ttctcgttca tatcgcttcc ttaaagttga aactcgcaca
     5041 tattggcggt atgcaagcag ggtttaaatt ttgtaaacgc ccattctagc agattgtcaa
     5101 caaaatcaga aaaatttaca tcgccgcgcg gctgcggcgt tagaatcgca ttttgtttgg
     5161 agcaaacacg atgaaacagc ctgtttttgc cgttacttcc ggcgagcctg ccggcatcgg
     5221 ccccgatatt tgtttggact tggcgtttgc acgcctgccc tgccgctgcg cggtattggg
     5281 cgacaaaaac ctattgcgcg cgcgcgccga agccttgggc aaaagcgtcg tcctgcgcga
     5341 cttcgatcca gaatcaggcg gcgcggcata cggcgagctg gaagtgctgc acatccctgc
     5401 cgtcgaagcg gttgaggcgg gcaaactcaa tcccgccaac gccgcctatg tgctgcaact
     5461 tttggacacc gcgctcgcag gcatttcaga cggcattttc gacggcatcg ttaccgcgcc
     5521 gctgcacaaa ggcatcatca acgacgcgcg cgcaagcaca ggttttttca gcggacacac
     5581 cgaatatctg gcggaaaaaa gcggcacggg gcaggtcgtg atgatgcttg ccggcaaagg
     5641 cctgcgcgtc gccctcgtaa cgacccacct gccgctgaaa gacgttgccg ccgccatcac
     5701 gcaaccgctg attgaaagcg tcgcacgcat tttgcatcac gacttaaaac acaaattcgg
     5761 catcaaaaat cccaaaatcc ttgtcgccgg acttaatccc cacgccggcg aaggcggaca
     5821 cctcggacac gaagaaaccg acaccattat ccctgcattg gaaaacctgc gccgcgaagg
     5881 gataaacctt gccggcccgt atccggcgga cacattgttc cagccgttta tgctcgaagg
     5941 tgcggatgcc gtattggcga tgtaccacga ccaagggctg cccgtgttga aataccacag
     6001 tttcgggcag ggcgtgaaca tcacgctggg tctgcccttt atccgcacct ctgtcgatca
     6061 cggcaccgcg ctcgatttgg cggcaaccgg cagggcggat tccggcagcc tgataactgc
     6121 cgtggagacc gccgtcgaga tggcgcgcgg cagcctttaa agatgataaa agacccgtca
     6181 tttccgcgca ggcggcatcg tccaccctcc gccgtcattc ccgcgcaggc gggaatccag
     6241 accttcgggc ggcggcaata ttcaaagatt atctgaaagt ttgaggttct agattcccgt
     6301 tttcacg
//
LOCUS       DAXHKX010000115         6274 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHKX010000115 DAXHKX010000000
VERSION     DAXHKX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.115"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(32..1375)
                     /gene="argG"
                     /locus_tag="ACFMWZ_001566"
     CDS             complement(32..1375)
                     /gene="argG"
                     /locus_tag="ACFMWZ_001566"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC5935508.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            1500..2300
                     /locus_tag="ACFMWZ_001567"
     CDS             1500..2300
                     /locus_tag="ACFMWZ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC5935509.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFVFFKSLLESKGLKLNQVQYLP
                     DEPDLLVKQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHAQAAGFIEG
                     VTLKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTQRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            2340..3326
                     /locus_tag="ACFMWZ_001568"
     CDS             2340..3326
                     /locus_tag="ACFMWZ_001568"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC5935510.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            3983..4245
                     /locus_tag="ACFMWZ_001569"
                     /pseudo
     CDS             3983..4245
                     /locus_tag="ACFMWZ_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4283..4657)
                     /locus_tag="ACFMWZ_001570"
     CDS             complement(4283..4657)
                     /locus_tag="ACFMWZ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC5935511.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISSDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(4654..4782)
                     /locus_tag="ACFMWZ_001571"
     CDS             complement(4654..4782)
                     /locus_tag="ACFMWZ_001571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935512.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            complement(4830..5186)
                     /locus_tag="ACFMWZ_001572"
     CDS             complement(4830..5186)
                     /locus_tag="ACFMWZ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC5935513.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(5191..5295)
                     /locus_tag="ACFMWZ_001573"
     CDS             complement(5191..5295)
                     /locus_tag="ACFMWZ_001573"
                     /inference="COORDINATES: protein motif:HMM:NF018014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935514.1"
                     /translation="MDDIRGIIQGAVNPLIYKVSKQKIIYNNKLISGT"
     gene            complement(5329..5672)
                     /locus_tag="ACFMWZ_001574"
                     /pseudo
     CDS             complement(5329..5672)
                     /locus_tag="ACFMWZ_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5831..6232)
                     /locus_tag="ACFMWZ_001575"
     CDS             complement(5831..6232)
                     /locus_tag="ACFMWZ_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935515.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
BASE COUNT         1699 a         1416 c         1424 g         1735 t
ORIGIN      
        1 ttaagttgct aatgatgcaa aacagcaaac cttattgctt attgcccaac tgcggcaata
       61 ccgagccttc gcccagcgac agcaaaccgc tttgcgagta gatgcccagt ttggcgcggg
      121 tgtcggtgat gtcgaggttg cgcatcgtga gctgtccgat gcggtcgagc ggggtgaacg
      181 cagcgtcttc gactttttcc atactcaggc gttcaggttg gtaggtcagg ttgggcgatt
      241 cggtgttcag aattgagtag tcgttgccgc gccgcagttc gagggtaact tcgccggtaa
      301 cggctttggc aacccagcgt tgggcggttt cgcgcaacat cagggcttgg ctgtcaaacc
      361 agcggccttg gtagagcaaa cggccgaggc gcaggccgtt gatgcggtat tgttcgatgg
      421 tgtcttcgtt gtggatgccg gtcaccaagc gttcgtaggc gatgtggaac aacgccattc
      481 ccggggcttc gtagatgccg cgcgatttgg cttcgatgat gcggttttcg atttggtcgc
      541 tcatgcctaa gccgtggcgg ccgccgatgc ggttggcttc gaggaagagt tcgacggggt
      601 cggcgtattc tttgccgttt aatgcgacgg gcacgccttc ttcaaagcgc acgctgactt
      661 cttcgggttc gatttcgacg ttttcgtccc aaaaggcaac gcccataatg ggtttgacga
      721 ttttgatgcc cgagttcaaa aattccaagt ctttggcttc gtgggtcgcg ccgagcatat
      781 tggaatcggt ggagtaggct ttttcaaccg acattttgta gttgaagccg ttggcaatca
      841 gaaattcgct catttcgtga cggccgccga gttcgtcgat aaattgctgg tcgagccagg
      901 gtttgtagat tttcagcgcg ggattggtga gcaaaccgta gcggtagaaa cgctcgatgt
      961 cgttgccttt gtaggtgctg ccgtcgcccc agatgtttac gtcgtcttct ttcattgcgg
     1021 aaacaagcat cgtgccggtt acggcgcggc ccagaggcgt ggtgttgaaa taggcgatgc
     1081 cgccggtgga aacgtggaac gcgccgcatt ggatggcggc gatgccttcg tgtgccaact
     1141 gcgcgcggca gtcgattaag cgggcgtttt ccgcaccgta ttccatcgct tttttgggaa
     1201 tggcgttgta gtcgtcttca tcgggctgac cgaggttggc agtgtaggca taaggcagtg
     1261 cgcctttgag tttcatccac aacagcgcgg cggaggtatc aagtccgccg gagaaggcga
     1321 tgccgacttt ctgaccgacg gggaggtttt gtaaaatagt atggttttgg ttcatgaaaa
     1381 aatcctcggt atcttgaaaa atcaaagcca ttatcttata aataggaaat attgtcagcc
     1441 atcatgccgt ctgaacgggt attccgccgt ttcaggcggt atttaaaacc gaaaacatta
     1501 tgaacgcgtt caaccttatc atcatcggcg acgaaatctt acacggcagc cgccaagaca
     1561 agcatttcgt ctttttcaaa tccctgctcg aatccaaagg gctgaagctc aatcaggtgc
     1621 aatacctgcc cgacgaacct gatttgttgg tcaaacaact gcgccgcagc ttttcagacg
     1681 gcatgccaac tttcgttacc ggcggtatcg gcgccacgcc cgacgaccac acccgccaag
     1741 ccgcagccgc tgctttggat ttgcccgtcg tccgccacgc gcaagccgcc gggtttatcg
     1801 aaggcgtgac ccttaaacgc ggtgagccgc tcgattcgcc cgaacacgcc caacgcctga
     1861 agatggcgga ttttccccaa ggcgcggaat tggtgcctaa tccgtttaat aatatcgccg
     1921 gattttccat tcgcgagcat tatttcttcc ctggttttcc tgtgatggcg cacccgatgg
     1981 cggaatgggt attggaaact tattatgccg accgcttcaa ccaaacccaa cgcggcagcc
     2041 gcagcgtgta tgtgttcgag cagcccgaat cgcgcattac gccgcttatg gagcatatcg
     2101 agcaaactta tcccggcgtg cgttcgtaca gcctgcccag cgttggttgg acgcattcag
     2161 acggcacgca ggtcaaaccg cacattgagt tcggcatcaa agccgaagga gaagccgtca
     2221 accttttgga cgcggcgtgg gcggaagtgt tgcacagctt ggacggattg ggagcagagc
     2281 tgaaaaatcg ggtaaactga aaaccgtttg cccaaatcgg acgatattta aggaaaacca
     2341 tgcaataccg aatcagaaga gaaaacgaag cacccgaagc aaaaaatacg ggggaaacct
     2401 tatgggagcg cgacatcatg cgcgaagtgc tgctgtccgc ctatcaggac aggcgcaggg
     2461 agcggatgtg gaaaaacatc tggcgcgccg tcagcaccct gattctggtt gccctgattg
     2521 caggcatttt ccgaaaagac gaagccgcat tgcagttggc gggcaacacg ccgcataccg
     2581 cagtcgtcaa tctgtacggc gaaatcggca acggtgtaga agaccaggtc aaaaaactca
     2641 aagacggtat ggaggccgcc tacaaaaacc cgcaggcaaa agccatcgtc atccgcgcca
     2701 acagccccgg cggttcgccc gtcgtctcca acaccgcttt tgaagaaata cgccgtctga
     2761 aggcgcagca ccctggcatt cccgtttatc tcgtggcgga agatatgtgc gcgtccggct
     2821 gctactacat cgcggcggcg gcggacaaaa tctatgccga cccgtccagc atcgtcggca
     2881 gcatcggcgt tatcggcagc agtttcgacg cgaccggcct gatggaaaaa atcggcgtga
     2941 aacgcagggt taaaattgca ggcagcaaca aaggcatggg cgatccgttt tcgcccgaaa
     3001 cgcccgaaca gtcaaaaatc tgggaagaaa tgctgaccgg catacacggt gagttcatca
     3061 aagccgtcaa aaccggacgc ggcggccgcc tcaaattccg acaatatccc gacgtgttca
     3121 gcggtcgcgt gtacacaggc gcggatgcgc tgaaagtcgg gctggtggac ggactcggca
     3181 atatttacag cgtggcgcga gacgtggtca aagcccccga tgtggtggat tacactccga
     3241 aggacgattt cggcagaatc ttgggcagac gcttcggcgc agaactcaaa gcaagcgtgc
     3301 gcgaagccct gcaggccgtc cggtagcgtt gtttacagta tttctaaggg aataccaaaa
     3361 taaaaatata cacaaaaaca ctcacctcct cagtatgctg ccactaagtc agccgcgtca
     3421 ctatttaccg tgcacttaaa gccgtaaaag gcaggttgtc cccgggaggt tttgtgcatc
     3481 ctgataccga tcggttgttg ttgctcaaag gacagaaggc cgctgataaa cgagattacc
     3541 tgtttgtcgc tattgacgat ttttatactc tgccattttg ccggacaaaa ccgcagacag
     3601 tgctgccaag tttctgaccg aacatctggc cgacccctgc ttgtacctga ttgagtacgt
     3661 ttactctgac aatgataggt aatataaaga gccgtccaac atgctttcgg tgcagcttgt
     3721 tatgataatg ggattggttg gaggcttgcc cgatttgctt gtccgcagac caacggtaag
     3781 gcggagcggg ttatccgtac cttgatggag atgtggcatg aggaacagtc gtttgacagt
     3841 ccggaacatc ggcaaaagga gttgtgctgc tttattaatt taggggctgc cctagatcat
     3901 tagggaaatt caaattaagt tagaattatc cctatgagag aaagtcgtct aagccggtat
     3961 aaacaaaata aactcatttt gttatcgtgg ctatgatgta ttggatgcgg gcgaatttag
     4021 ccatttccgt atcaatcaca gcacacattt tgctgaacga caaaaccata ttaatggaac
     4081 tgagaacttt tggaaccagg caaaacgtca tttacgcaag tttaacggca ttcccaaagc
     4141 gcattttgag ctgtatttaa aggagtgcga atggcgtttt aacaacagtg agataaaagt
     4201 tcttgttcca ttttaaaaca attagtaaaa tcgagtttat cctagttgtc cagggcagcc
     4261 tcatctcttt atagatagct gcttaccaag tatccttaat tgctaattca agccaatctt
     4321 caaaactatt aaaaaattca tggttttcta tattatctaa attaatgaca ataaccctag
     4381 gatttcctgt tacatcatta ccatcaaaat aagcttccca ttctccatta ctagcgaatg
     4441 ggataagatt taaatcaggc aggatagaat ttctaaatat ataagctact tctgcccctt
     4501 ctgttccata attctcaaac caccagtgaa aattatgtgg ttgaatataa ggtattaaag
     4561 atgtatcgct agagatacga acatactttt caggataaca aaacctttca ggaagcatat
     4621 tactaggata taaattaaat tcattttgaa taatcatttc gttaccttag gatcataaat
     4681 actaccatta ggaaactggg tagaccaatt tggtggtgct agttcatttt caatttgttt
     4741 tttaattcta aattgaatag cttctcccca tgtttgcggc atctaatgct gcttgctctg
     4801 catcaatatt actaaaaatt ggattagtct caaaaatgaa aaattactgt tttttcattt
     4861 tttatagcat attcaagtac atgaataata tttttcacta agggctctgt agaaaaatct
     4921 gaattttgta atttttcttt tacagccaaa atactttcct tatctattag ccaatccccc
     4981 tcacttggtc caaataacca attatcggta atttcagaaa caagtctcca taatccacga
     5041 ttttgatatt caataatttg attttccgta atttctacaa ctgtttcaga ttgaatgttt
     5101 ttataagccc aatcaatcat ttcagtatat tcactaattg aaacattgtc ttttaaacag
     5161 aaaattaaca ggttgtgttc attcatttgt ctaagttcct gatatgagtt tgttgttgta
     5221 aataatcttt tgcttgctta ccttgtaaat taaaggatta accgcacctt ggatgatgcc
     5281 gcgaatatca tccatacggt taccccacca atttttattg gtgtatcgtt aatcatttat
     5341 ttcaaaaaag ttatttatta tcatattatc agggagcagg tcgttaaaaa aaccccctca
     5401 aattccatta aattagtatt tatccagcac ataaacgaac caaagcgttc tttccctgag
     5461 tgtaaatatt taaaattaaa ctcaaaataa gtttttttgc ttagaaaatt taatggtttt
     5521 tctatagtga aaaaaaagcc cttactcgtt ttcgttcgag atactatttt aaataaattt
     5581 ttattttcaa taaaataaga atattcattt actaagttgt tatgataaaa aagtaatttt
     5641 attaaaatgt tttcataatt ttctaggttc atttctagct caatttaaaa tgatttcatt
     5701 gttgccgtac caatgcgaaa tttaccatca acaaaatttc ccttcactga tacaggtaca
     5761 ccatcaactt gggtagtttt attttcaggc tgcctgaaag aaagataggc aacttattac
     5821 tcatcttatg ctagccctca ataattgaat acgttgcact agaaccaatt atttctatga
     5881 atgtttgctc aaacataaaa atatagtatt taaagaattc tctattcaaa ggaaaataac
     5941 tttctttcaa tatccattcc gcataacact catcaagaag ttcaaaaaaa gattgaccac
     6001 cattttcatg aagcaaagct ctatctgcat cttccgaaat taaatccata aaagtatcta
     6061 taaaatacga ttcatcaaca accctaaaat gaataacatc ttcaataaaa atttttatgg
     6121 ttttatccgt atttctataa ccaactgcta taacttctac tttatcagag tttattgctg
     6181 cattatcaaa atagaaatag ccagttggga aatctaagct ttttaatttc ataatttatc
     6241 gtgtatttcc atatctgatt tttatttttc ggtt
//
LOCUS       DAXHKX010000116         6267 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHKX010000116 DAXHKX010000000
VERSION     DAXHKX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.116"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..151)
                     /locus_tag="ACFMWZ_001576"
     CDS             complement(<1..151)
                     /locus_tag="ACFMWZ_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC5935516.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YSGLTK"
     gene            274..1227
                     /locus_tag="ACFMWZ_001577"
     CDS             274..1227
                     /locus_tag="ACFMWZ_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC5935517.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVHD
                     AHVVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1302..3359)
                     /gene="metG"
                     /locus_tag="ACFMWZ_001578"
     CDS             complement(1302..3359)
                     /gene="metG"
                     /locus_tag="ACFMWZ_001578"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC5935518.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHMQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGCLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPKVAEN
                     AAKFLNLEAITWANTRETLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(3475..5313)
                     /gene="glmS"
                     /locus_tag="ACFMWZ_001579"
     CDS             complement(3475..5313)
                     /gene="glmS"
                     /locus_tag="ACFMWZ_001579"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC5935519.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKSGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPSDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
     gene            5595..6113
                     /locus_tag="ACFMWZ_001580"
     CDS             5595..6113
                     /locus_tag="ACFMWZ_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935520.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1343 a         1483 c         1884 g         1557 t
ORIGIN      
        1 tttttgttaa tccactatag aaaacgacgg tgttgattag gggctcgacc agcgcgaccg
       61 cccccgatac gcctatactg cccgtcagta cataggttac actgaaggcg gtgctgaaat
      121 gcagtgcggc aaaagtcaag gttttaagca tcatcatctc ccgaattgaa aatttacgga
      181 gagatgataa agattatcat caggccgtgc cggtttaaat ttgctattta ctgttagtgt
      241 aaataaatca attttaaagt aaggatagaa atgatgaatc acaaaaagat agtcgttctg
      301 gatgcggata ctttgcccgg ccgggttttt cattttgatt ttccgcacga gctcgcagtt
      361 tacggtacga caggtgcgga tgaaacggca gaacgggtgc acgacgcaca tgttgtcatt
      421 accaataagg taatgatttc cgccgacatt attgcggcta atccgcagtt ggagctgatt
      481 gccgtcagtg cgaccggtgt gaacaatgtc gatattgggg cggcggaggc ggcgggcgtt
      541 gcggtatgca atgtccgcgc ctacggaaac gaatcggttg cggaacacgc ctttatgctg
      601 atgattgcgt taatgcggaa tctgcctgcc tatcagcgtg atgttgcggc cggtttgtgg
      661 gaaaagtcgc cgtttttctg ccattacggc gcgccgattc gggatttgaa cggcaaaacg
      721 ctggcggttt tcggacgcgg caatatcgga cggacgcttg ccggatacgc gcaggcattc
      781 ggtatggggg tggtgtttgc cgaacacaaa cacgcgtccg ctgtgcgcga aggctatgtt
      841 tcctttgaag atgcggtacg ggctgctgat gtgttgtcgc tgcactgtcc gctaaacgcc
      901 caaactgaaa atatgatagg cgaaaacgaa ttgcggcaga tgaagcctgg cgcggtttta
      961 atcaattgtg ggcgcggcgg gctggtggat gaaaacgcgc tgcttgccgc actcaaatac
     1021 gggcagatcg gcggggctgg tgtcgatgtt ttgacggagg agccgccaag gggcggcaat
     1081 cccctgctga atgcacgact tcccaatctg attgttacgc cgcataccgc gtgggcgagc
     1141 cgcgaggctt tggacaggct gttcgatata ttgttggcga acattcacgc ctttgtgaaa
     1201 ggcgaggcgc aaaaccgggt agtttgagct tgtcgggata acgaaaaaca atgccgtctg
     1261 aacgcccaaa gggttcagac ggcatttttt gagatttcta cttaaccgac tttgtcgccc
     1321 ggttgcgcgc ctgcatccac atccaagagc ttcagtttcc cgtctgccgt ggcagcggag
     1381 agaatcatgc cttcggatac gccgaatttt gccattttgc gcggggcgaa gttggcgacg
     1441 gcgatgacca tgcggccgtt caattcggca ggatttgggt aagacgcggc gatgccggag
     1501 aagatgatgc gtttttcaaa accgaaatcg aggtcgaatt ttaagagttt ggtgctgcct
     1561 tcgacggctt cgcagttcaa tactttggcg acgcgcatat cgattttcat aaagtcgtcg
     1621 aagctcgcct gttcggcgac tttttcgtat ttgctctctt cggcggcagg cgctgctgct
     1681 gctgcgatac tttgtttgtt ggcttcgatt aaatcgtcca cttgtttttg ctccactcgt
     1741 tgcattaaat gttcgtattt gttgatggcg tgtttgccca aggtttcgcg tgtatttgcc
     1801 caagtgatgg cttccaaatt caggaatttg gcggcatttt cggccacttt cggcaacacg
     1861 ggggcgaggt aggcggtcag catggtaaag gcgttgatga gttcgctgca tacttcgtgc
     1921 aggcgttcgt cttggccttc ttgtttggcg agttcccaag gtttgttggc atcaacgtat
     1981 tcgttgacaa tgtctgccaa ggccatgatg tcgcgcaggg ctttggcgta ttcgcggctt
     2041 tcgtagcatt cggcaatggc ttcgctttgt gcggtcagtt ttgccagcaa tgcgctgtcg
     2101 gcaacgtctt tcagacagcc ttcaaagcgt ttggcgatga aacctgaggc gcgggcggcg
     2161 atgttgacgt atttgccgac gaggtcgctg ttgacgcggc tgataaagtc ttgcaggttc
     2221 aaatcgatgt cttcgatttt gctgttgagt ttggcggcga tgtagtagcg catccactcg
     2281 gggtttaaac cttgttccag ataggatttg gcggtgataa acgtgccgcg cgatttggac
     2341 attttttgtc cgtctacggt caaaaagccg tgtgcgtaca cgccggtcgg ggcgcggtgg
     2401 ccggagaaat gcagcatggc aggccagaac agggcgtgga aatagaggat gtctttgccg
     2461 atgaagtggt acatctcggt ttggctgtcg gctttgaagt attcgtcaaa atcgacgccg
     2521 atgcggtcgc acaggttttt aaacgacgcc atgtagccga cgggcgcgtc cagccagacg
     2581 tagaagtatt tgcccggcgc gtcggggatt tcaaaaccga aatacggcgc gtcgcgggaa
     2641 atatcccagt cggacagggt ggtttcttca ccttcgccca gccattcttt cattttgttg
     2701 agggcttcgg cttgcatatg gggcttgccg tcgtgcgggt tgttgccgga agtccatgct
     2761 ttgaggaagt cggcgcattc tcccagtttg aagaagaagt gttcggattc gcgcaattcg
     2821 ggtttcgcgc cggaaacggc ggaatacggg ttaatcagtt cggtcgggga ataggtcgtg
     2881 ccgcagactt cgcagttgtc gccgtattgg tcttgggcgt ggcatttcgg gcattcgcct
     2941 ttaacgaagc ggtcgggcag gaacatttgt ttttcggggt cgaaaagctg ctcgatgacg
     3001 cggctctcaa tcttgccgtt ggctttcagc gcgcggtaaa tgtcttggga aaactgtttg
     3061 ttttcagggg aatgggtgct gtaataattg tcgtaaccga tgaaaaagcc agtaaagtcg
     3121 gcgaggtgct cttcgcgcac tttggcaatc atgtcttcgg gcgcgatgcc ttgtttttgc
     3181 gcggcaagca tcacgggcgt gccgtgggtg tcgtcggcgc agcagtagtg gcattcgttg
     3241 ccgcgcagtt tttgaaagcg cacccaaacg tcggtttgga tgtgttcgac catgtggccg
     3301 aggtggatgc tgccgttggc atagggcagg gcggaggtaa cgaggatttt gcgtgtcata
     3361 ttgtgctttg caaacaatgg gtaaaggcgg attataccgc aaatcaaacg gggaaatgcc
     3421 gtctgaagcc tgaaaaatcg ggcttcagac ggcatgttcg caaactatcc ggacttattc
     3481 gaccgtaacg gatttcgcca ggttgcgcgg tttgtccaca tcagtaccgc gtgcgagggc
     3541 gacatggtag gaaaggagct gcacggggat agtatgcacg acgggggata attctccgac
     3601 atggcgcggt gcgcggataa cgtgcacgcc ttcggtagca ttaaaattgc tgtcgaggtc
     3661 ggcaaagacg aaaagttcgc cgccgcgcgc gccgacttcc tgcatattgg ctttgacttt
     3721 gtccaacagg ctgtcgttgg gcgcgatgac gacgacgggc atgttttcgt ccactaaggc
     3781 aagcggcccg tgcttcagtt cgccggcagg ataggcttcg gcgtggatgt aggtgatttc
     3841 cttcagtttt aacgcgcctt cgagggcaat cgggtaatgg atgccgcgcc ccaaaaacag
     3901 cgcgctggtt ttcttggcaa actgttgcgc ccaggcggca atttgcggtt cgaggtttaa
     3961 agcgtgctgc acgctgccgg gaagctggcg gagttcttcg gtgtaacgtg cttcgtcttc
     4021 ttcggaaacc aaaccgcgca ctttcgccag cgttaccgcc aaaccgaaca gcgcaaccag
     4081 ctgcgtggta aatgctttgg ttgaggcgac gccgatttcc gcaccggcac gggtataaag
     4141 cacgaggctg ctttcgcgcg gcagggcgga ttccatcacg ttgcagatgg agaggctgtg
     4201 gcggtgtccc aaggattttg cgtatttcaa cgcctccatc gtgtccagcg tttcgccgga
     4261 ttgggaaatg gtaatgacca gttggtccgg atcggcaatc acgctgcggt atcggtattc
     4321 gctggcgatt tcgacatcgg acgggatttt ggcgatggat tccaaccaat atttggcggt
     4381 cagcgcggcg taataggacg tgccgcaggc aaggattttg acgctgcgga tgctttcaaa
     4441 cacgcttttg gcgtttttgc cgaagttttc ggggatgaag ccgccgtcga ggaaaacttc
     4501 cgccgtgtcc gcaatcgcgc gcggctgctc gtggatttcc ttttgcataa agtggctgta
     4561 cggccccagc tccaaagagg cgagcgagag ttcggatacc ttgactttgc gttcggcggg
     4621 caggccgctt ttatcggtca gccttttgat gccgtctgaa gccagcagcg cgatgtcgcc
     4681 gtcttcgagg tacgccacgc ggcgcgtaaa ggcgatgacg gcggatacgt ccgaagcgat
     4741 aaaggtttca tcgtcgccca aagcgaccaa aagcgggcag cccatacgcg ccacaactaa
     4801 ttcatcaggc ttgtcttggg caataaccgc gatggcgtat gcgccgtgga aacgtttgac
     4861 cgcttcctgc acagcttcaa acaattttcc gccgttttgc gcgtattcgt gattgatgct
     4921 gtgtgcgatg acttcggtat ccgtttgcga ttcaaaacgg tatcccaaac cttccaagcg
     4981 tttgcgttcg ctttcaaagt tttcgatgat gccgttgtgg acgaccgcaa tcatcccgcc
     5041 gctgatgtgt gggtgggcgt tcggctcggt aacgccgccg tgtgtcgccc agcgcgtatg
     5101 cccgatgccg atgccgccgc tgatgccttt ttcgcgtgct gcgtcttcca taagctgcac
     5161 gcgtccgacg cggcgcacgc gtttgatttt gccgtcggtg ttgacggcaa tgcctgatga
     5221 gtcataaccc cggtattcga ggcgtttgag accgtcggtc agaaaatcga cgacgttgtg
     5281 atgggcgcgg atggcgccga cgataccgca cataactgtt ccttagtatc cggttgaaaa
     5341 aaaacaggcg cggacggctt ccgtgccgca ccttcctctt cggattataa accgccttcc
     5401 gcgccgaaaa acagcaaaat gccgtctgaa ggcttgggct tgctcaaaaa aaggagggat
     5461 ttccccgcct gaccgggatg ggcgttcaga cggcattgcc tgctgccggt ttatagtttt
     5521 tacaaatcaa tattgacaag ctgaaaaaaa aaaacaatat actcgctcgg tcttaatgtt
     5581 aacggagtat ggaaatgaaa caaatgcttt tagccgtcgg cgtggtggcg gtgttggcgg
     5641 gctgcggcaa ggatgccggc ggttacgagg gttattggcg cgaaaaatcg gacaaaaaag
     5701 agggtatgat tgccgtcaaa aaagaaaaag gcaattactt ccttaataaa atcaacgtgt
     5761 ttacaggtaa ggaagaatct atgcttttgt ctgaaaaaga cggcgcgctt tcgataaaca
     5821 cggggatagg ggaaatcccg atcaaacttt ccgacgacgg gaaagagctg tatgtcgaac
     5881 gcaggcagta tgtcaaaacc gatgcggcga tgaaggacaa aatcatcgcc catcagaaaa
     5941 agtgcggaca aacggcacag gcatatctcg acgcgcgaaa tgcgttgccg tcaaaccaga
     6001 cgtatcagca gcatcaggcg gcgatcgagc agttgaaacg gcggtttgaa gccgagtttg
     6061 acgaattgga aaaagaaatc aaatgcaacg gcaaaccgac attgttgttt tagcgtagga
     6121 caaccgggag gatgccgccg tccgaatcgg acgtgcggtt tctgtaccgg tacgggcggg
     6181 caggaatgtc cgcttttttt gttcggacgc gtttgaatac ccgtttgatt ccgtccgttt
     6241 gcaaagggta tttttgttcg ggcggaa
//
LOCUS       DAXHKX010000117         6248 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHKX010000117 DAXHKX010000000
VERSION     DAXHKX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.117"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..2091
                     /locus_tag="ACFMWZ_001581"
     CDS             <1..2091
                     /locus_tag="ACFMWZ_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC5935521.1"
                     /translation="AGVGEQARADAKIILEGIKSSIHTETVSSSKSTLWQKQAGRGSN
                     IETLQLPSFTGPVAPVLSAPGGYIVDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNIN
                     WNQVQLAYDKWDYKQEGLTGAGAAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATT
                     TAAATTTVSTAAAMQTAALASLYSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSAL
                     TAGALNQMGADIAQLNSKVRTELFSSTGNQTIANLGGRLATNLSNAGISAGINTAVNG
                     GSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKD
                     GAIGAAVGEIVAESILGGRNPATLSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAA
                     EVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKIIQGIMPAHAAGAMTNPQDKDAAIW
                     ISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKLAISTCMANPSGCTVMVTQAAEAG
                     AGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETINNLANLAKAEQQILFRIAQRDT
                     QLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFA
                     LYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNP
                     PSFIKGTKLELKFQ"
     gene            2110..2484
                     /locus_tag="ACFMWZ_001582"
     CDS             2110..2484
                     /locus_tag="ACFMWZ_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC5935522.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            2843..5158
                     /locus_tag="ACFMWZ_001583"
     CDS             2843..5158
                     /locus_tag="ACFMWZ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC5935523.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKLQSYGDQFYYAGQGELYTFDKRSYKTGKWYKLKHVTEIKEHKNAKADPVSLSASQG
                     IEIKSGGNIGAHATLFDAPRGSVKIEAGRGLVLYAVEDLNYDKLDTRTKRKFIGITYD
                     KVHDTTTHTMKTALPSRVVAESANLQSGWDAKLQGTQFETTLGGAAIRAGVGDQARAD
                     AKIILEGIKSSVRTETVSSSKSALWQKQAGRGSNIETLQLPSFTGSVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQTAKNVDWKQVQLVYDKWDYKAEGLTGAGAS
                     IIALAVAAATGGVGAGLIGAAQGTTSAAIANAAFTSLATQASISLVNNKGNVGKTLKE
                     LGRSRTVKNLVVAAATAGVSNKIGTSALANVSDKQWVNNMTVNLADAGSAALINTAVN
                     GGSLKDNLEANILAALVNTAHGEAASKIKGLDQHYVAHKIAHAVAGCAAAAANKGKCQ
                     DGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNT
                     AAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLT
                     TLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMST
                     YMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFD
                     KNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            5167..5544
                     /locus_tag="ACFMWZ_001584"
     CDS             5167..5544
                     /locus_tag="ACFMWZ_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935524.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            5822..6196
                     /locus_tag="ACFMWZ_001585"
     CDS             5822..6196
                     /locus_tag="ACFMWZ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935525.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDKLEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
BASE COUNT         1957 a         1323 c         1490 g         1478 t
ORIGIN      
        1 gcaggcgtag gtgagcaggc acgggcagat gccaagatta tcctcgaagg gatcaaaagc
       61 agcatccaca cagaaaccgt gagcagcagc aaatctactc tatggcaaaa acaggcagga
      121 cggggcagta acatcgaaac cttgcaattg ccgagtttca ccggtcccgt tgcgcccgta
      181 ctgtccgcac ccggcggtta cattgtcgac attccgaaag gcaatctgaa aacccaaatc
      241 gaaaccctca ccaagcagcc cgagtatgct tatttgaaac aacttcaagt tgcgaaaaac
      301 atcaactgga atcaggtgca gcttgcttac gataaatggg actataaaca ggaaggcttg
      361 accggagccg gtgcagcgat tgtggtgatt attgtaaccg ctctaactta tggatacgga
      421 gcggctgcag cgggtagcgt aactgccgca ggaagtagta cagccgcagc tgcaacaacg
      481 acagcggcag caactaccac tgtttctact gcagctgcca tgcaaaccgc agctttagcc
      541 tccttgtata gccaagcagc tgtagccatc atcaataata aaggcgatgt aggcaaagca
      601 ttaaaagatc tcggcaccag tgatacggtc aagcagattg ttacttccgc actgacggcg
      661 ggtgcattaa accagatggg agcagatatt gcccaattga acagcaaggt aagaaccgaa
      721 ctgttcagca gtacgggcaa tcaaaccatt gccaaccttg gaggcagatt ggccaccaac
      781 ctcagcaatg caggtatctc agctggtatc aataccgccg ttaacggcgg cagcttgaaa
      841 gacaacttag gcaatgccgc attgggagca ttggttaata gcttccaagg ggaagccgcc
      901 agcaaaatca aaacaacctt cagcgacgat tatgttgcca aacagttcgc ccacgctttg
      961 gcgggttgtg ttagcggact ggtacaggga aaatgtaaag atggggcaat tggcgcagca
     1021 gttggggaaa tcgtagctga atccatactt ggcggcagaa accctgctac actcagcgat
     1081 gcagaaaagc ataaagttat cagttactcg aagattattg cgggcagcgt ggcggcactc
     1141 aacggcggcg atgtgaatac tgcggcgaat gcggctgagg tagctgtggt gaataatgct
     1201 ttgaattttg acagtacccc taccaatgcg aaaaagcatc aaccgcagaa gcccgacaaa
     1261 accgcactgg aaaaaattat ccaaggtatt atgcctgcac atgcagcagg tgcgatgact
     1321 aatccgcagg ataaggatgc tgccatttgg ataagcaata tccgtaatgg catcacaggc
     1381 ccgattgtga ttaccagcta tggggtttat gctgcaggtt ggacagctcc gctgatcggt
     1441 acagcgggta aattagctat cagcacctgc atggctaatc cttctggttg tactgtcatg
     1501 gtcactcagg ctgccgaagc gggcgcggga atcgccacgg gtgcggtaac ggtaggcaac
     1561 gcttgggaag cgcctgtggg ggcgttgtcg aaagcgaagg cggctaagca agctgctcct
     1621 aaagaaacaa taaacaattt ggcaaattta gccaaagcag aacagcagat tttattccgt
     1681 attgcccaac gcgatacgca actggatgca tggaagacgg gatttaacaa tagagtaagg
     1741 aaaggagcag gcttgcttga tgcaagtaat attccgataa ccattaacgg aaaaaccatc
     1801 aaacctgtac aagccataag cttaaaggga gcacccgttt acagcggcgt aagcgaacag
     1861 gagatttttg cgctttatcg gcagatgact ggccagaatc cgaattttag agttttgcct
     1921 gacggaagat tagcaaatgg cattatcagt actggagaat gggcaggaac aaaaattgca
     1981 ttaagaaatt tttcaaaaac agagaattca actcaagcac gatggacatt agatttgcag
     2041 aatcctccat catttattaa aggtactaaa ttggagctta aattccaata atttacaaag
     2101 gattttaccg tggatgagaa acaaaaaatt aagattcttg attttcaaat cgatttatcc
     2161 tcaattttta actcttataa aaatcaaatg ggtattaata ttcaagataa aaacttaaaa
     2221 aacaatttct gttcttttat ggaagaactg ttaaatgacg gttcaatccg tttacatgat
     2281 tataccgacg gtatcggaat tcctctaact ggaacttcaa aagaacaagt gcagaaattg
     2341 aaagacatat ggcctacttt ggaagatgcc caagcaatat ggcctgaaga cccttggtat
     2401 tacttagaat ggctttggtg ggatattgcg tgtccaatag atttggccga tttgccgaat
     2461 attgatattt atgagcaagc gtaggtatgg ttagccgcct ttagcggcgt aaccgtacgc
     2521 atatcagcaa actttataaa ataacaaggc cgtctgaaat ctgtttttca actttttcag
     2581 acggccttgc aacttggcat ttcattcgta cggttacgcc gctaaaggcg gctaaccgta
     2641 cctacgagct ctgataaaaa tgatttatgg aagcaagctg tagcctgcat gaaacctaaa
     2701 atccatgcgt aaggtgtgtg cttcagcgcg cacgcgttcc atgatttacg gctcaatgcc
     2761 gtctgaaaag ctcaattttt tcagacggca tttgttatgc aagtaaatat tcagattctc
     2821 tgtatactgt tcagacgcgt gcgtgctgaa gacacctcct acgcttgctg cagaactttc
     2881 gggtaaaacc ggtgtgagca ttagcgcgcc gtatgccaat gaaaacagcc gcatcctgct
     2941 gagcaccacg gatatcagtt cggaaaacgg caaaatcaaa ctgcaatcct acggcgacca
     3001 gttctactac gccggacagg gtgagctcta caccttcgat aaacgcagct ataaaaccgg
     3061 taagtggtac aaactaaaac atgttactga aatcaaagag cataaaaacg ccaaagccga
     3121 cccggtgagc ctcagtgcgt cacaaggtat tgaaatcaaa tccggcggca atatcggtgc
     3181 ccacgccacc ttgtttgatg caccccgcgg ctccgttaaa atcgaagccg gacgtgggct
     3241 ggttctctat gccgtggaag atctcaacta cgacaaactt gacacccgta ccaagcgcaa
     3301 atttatcggc attacctacg acaaggtgca cgacaccacc acccacacca tgaaaaccgc
     3361 cctgccctca agggtagttg cagaatcggc caacctgcaa tcaggctggg acgccaaact
     3421 gcaaggcacc cagtttgaaa ccacgctggg cggcgcagcc atccgtgcag gtgtaggcga
     3481 tcaggcacga gcagatgcca agattattct tgaaggcatc aaaagtagtg tgcgcactga
     3541 aacagtaagc agtagcaaat ctgccctctg gcagaaacag gccggacgcg gcagcaatat
     3601 cgaaaccttg caactgccaa gtttcacagg ctccgttgcg cccgtactct ctgcccccgg
     3661 cggctatatc gttgatattc cgaaaggcaa tctgaaaacc gaaatcgaaa agctggccaa
     3721 acagcccgaa tacgcctacc tgaaacagct tcagacggcc aagaacgtcg attggaaaca
     3781 ggtgcagctt gtctacgaca agtgggacta taaagccgaa ggcctgaccg gagccggagc
     3841 ctccattatc gccctggcag tagcggcagc aaccggaggc gtcggtgccg gtttgatcgg
     3901 tgcagcacag ggaaccacat ctgccgccat agccaatgca gcatttacct cgctggctac
     3961 ccaagcttcc atttcgctgg ttaataacaa aggcaacgtc ggcaaaaccc tgaaagaact
     4021 gggcagaagc cgtacggtga aaaatctggt cgtggctgct gccaccgcag gcgtatccaa
     4081 caaaatcggc acgtctgctt tagccaatgt cagcgacaag cagtgggtca acaacatgac
     4141 cgtgaatctg gccgatgcgg gcagtgccgc gctgattaac accgccgtta acggcggtag
     4201 cctgaaagac aatttggaag cgaatatcct tgcggctttg gtgaataccg cgcatgggga
     4261 ggcggcgagt aagatcaaag gactggatca gcactatgtc gcccacaaaa tcgctcatgc
     4321 cgtagcgggc tgtgcggctg cagcggcgaa taagggcaaa tgtcaggacg gcgcgatcgg
     4381 tgcggctgtg ggtgagattg tcggggaggc tttggttaaa aataccgatt ttagcgatat
     4441 gaccccggaa caattagatc tggaagttaa gaaaattacc gcctatgcca aacttgcggc
     4501 aggtacagtt gcaggcgtaa cgggaggaga tgtcaatact gctgcacaaa ccgcacaaaa
     4561 cgcggtagaa aataatgcgg ttaaagctgt tgtaactgct gcaaaagtgg tttataaggt
     4621 agccagaaaa ggattaaaaa acgggaaaat caacgttaga gatttaaaac agacgttgaa
     4681 agacgaaggt tataatttag ccgacaacct gaccacctta ttcgacgaaa cattggattg
     4741 gaacgatgcc aaagccgtta ttgatattgt cgtcggaaca gagctgaatc gcgctaataa
     4801 aggggaagcg gcacaaaagg tcaaggaagt tttagaaaaa aatcgtcctt atatccctaa
     4861 taaaggtgct gtaccgaata tgagtacata catgaaaaat aatccttttg gaaaacagct
     4921 ggctcaaatt tcagaaaaga caacgcttcc gacgcagcaa gggcagtctg tcttcttggt
     4981 aaaaagaaac caagggttat taaaaaccgg tgataggttt tatttagatg gccaacataa
     5041 aaatcattta gaggtttttg ataaaaatgg gaactttaag tttgttctaa atatggatgg
     5101 ttcgcttaac caaatgaaaa ctggggcagc aaaaggtcgt aaattaaact taaaatagga
     5161 aattttatgg aaacattgaa tgatataaaa aaaatcttga ttaatgtggg gctttatcaa
     5221 gggtttgatt tgacagatcc aaaagtatca gaagaagtta atcatgaaac agctaatatg
     5281 aaatggatta aagattatac ttcagacggg aattgggata atgaatttaa ggaggattta
     5341 aaaaactttt tagattatat ggaagtatgc caattagccc taaacgataa aaatttcaaa
     5401 attgccagta attctttatt tatggctatg atttacgcag gtaatctatc tcttatattt
     5461 gattcaataa aaactgatat atcaacatta ttgagtgctg agtataaaaa gaatagtttt
     5521 tcatggccat ctcttgatga atagaaagca agttgtagcc tgcatgaaat ctaaaaccca
     5581 tgcataaggt gtgggcttca gtatacgcgt tccatgattt acggccatat gccgtctgaa
     5641 aagctcaatt ttttcagacg gcatttgtta tgaaagtaaa tatttagatt ccctgtatac
     5701 tgtttagact cgtgtgtgct gagtaagctg tagtctgcat gaaacctaaa actcgctcaa
     5761 aattaagcta agacattagc agggcaaggg cgaaaattga atcttaaata aggtgattca
     5821 gatgaaaaat tttaatgtag taaaagaaag tttaagagag ttaggaatta aacaaggatt
     5881 tgatctttat gagaaagcca caactgaaaa attgaatagt gaagatcctc ttgacttaca
     5941 atggctttct aactattcat ctgattggaa tgataaatta gaagaagact ttgattcttt
     6001 ttttcagcat atgaaggaat atcaatatgc tattgacaat gaagacatta aatctgcatg
     6061 tagttcacta tgtgaagcta tgctctatgt tggtaatatt aaaaattttt ttgagtttct
     6121 caaaagcgat atgattagac tgttgagagg tgaaagtaaa acaacagact ttcaatggcc
     6181 gcaatttgat gaatagcagc aagctgtagc ctgcatgaaa cctaaaatcc atgcgtaagg
     6241 tgtgtgct
//
LOCUS       DAXHKX010000118         6233 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHKX010000118 DAXHKX010000000
VERSION     DAXHKX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.118"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..449)
                     /locus_tag="ACFMWZ_001586"
     CDS             complement(<1..449)
                     /locus_tag="ACFMWZ_001586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC5935526.1"
                     /translation="MFRAQLGSNTRSTKIGDDADFSFSDKPKPGTSHYFSSGKTDQNS
                     SEYGYDEINIQGKNYNSGILAVDNMPVVKKYITDTYGDNLKDAVKKQLQDLYKTRPEA
                     WAENKKRTEEAYIEQLGPKFSILKQKNPDLINKLVEDSVLTPHSNTS"
     gene            complement(1919..2164)
                     /locus_tag="ACFMWZ_001587"
     CDS             complement(1919..2164)
                     /locus_tag="ACFMWZ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935527.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            2276..2704
                     /locus_tag="ACFMWZ_001588"
     CDS             2276..2704
                     /locus_tag="ACFMWZ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC5935528.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            2716..3192
                     /locus_tag="ACFMWZ_001589"
     CDS             2716..3192
                     /locus_tag="ACFMWZ_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC5935529.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKIMETMCPFKMEKRRYPVRWNGSLFEVD
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSKHPFSSWKNAV"
     gene            complement(3542..4030)
                     /locus_tag="ACFMWZ_001590"
     CDS             complement(3542..4030)
                     /locus_tag="ACFMWZ_001590"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC5935530.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLSGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(4098..5153)
                     /gene="aroG"
                     /locus_tag="ACFMWZ_001591"
     CDS             complement(4098..5153)
                     /gene="aroG"
                     /locus_tag="ACFMWZ_001591"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC5935531.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            complement(5315..5542)
                     /locus_tag="ACFMWZ_001592"
     CDS             complement(5315..5542)
                     /locus_tag="ACFMWZ_001592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935532.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            5819..6076
                     /locus_tag="ACFMWZ_001593"
     CDS             5819..6076
                     /locus_tag="ACFMWZ_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935533.1"
                     /translation="MTTQMGASRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYRH
                     KTICKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
     gene            complement(6058..>6233)
                     /locus_tag="ACFMWZ_001594"
     CDS             complement(6058..>6233)
                     /locus_tag="ACFMWZ_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC5935534.1"
                     /translation="LREMAEKLGHKVLPLASYSPELNPIEKVWANIKRYLRTVLSDYA
                     RFDDALLSYFDFN"
BASE COUNT         1571 a         1520 c         1437 g         1705 t
ORIGIN      
        1 tgtgatgtat tactatgagg agtgagtacg gaatcttcta ccaatttatt aattaaatcg
       61 gggtttttct gtttgagtat actaaatttt ggtccaagct gttctatata cgcctcctca
      121 gtccgttttt tattttctgc ccaagcttcg ggtcttgttt tgtataaatc ctgtaattgc
      181 ttcttaaccg catcctttaa attatccccg taagtatctg taatatattt cttaacaacg
      241 ggcatattat cgacggcgag tatgccgcta ttgtagtttt taccttggat attgatttcg
      301 tcatacccat attcggatga attttgatcg gttttaccgc tggaaaaata atgggaagtg
      361 ccgggtttcg gcttgtctga aaatgaaaaa tcggcatcgt cgccgatttt ggtagaacga
      421 gtatttgaac caagctgagc tctgaacata ccgaagcaat caaaagatat gctgcacggg
      481 ccgcgccaaa cgtgggaatt tctgtcttcc gccgcttcta aaataaattt gtttttaatc
      541 tcggcttctt tgacgggatc gtacgcatcc gaacctcaaa catatggcgt gcaaaacagc
      601 gataaaacag ataatgttaa tagattaacc cgaccgcttg cttgcttgct tgcttgcttg
      661 cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgctttca gtgtcatgag
      721 aaattttcct ttgtcaagtg ttaaaattat aatgattata tactacatta tactacatcg
      781 caatgaaaaa tcaggaaaag caaaaaaccc tccgccgtca ttcccgcgaa agcgggaatc
      841 tagaatttta atgccgcaag aatttatcaa ggatggtcgg ggtttaaagg tctggattcc
      901 cgcctgcgcg ggaatgacgg tttagaaatc acccgaaaca agaaaaagaa ccgaaaccga
      961 acaagccgga ttaccgcctg cgcgggaatg acgggatttc aggttactgt ttttgatttt
     1021 ctgttttgtg gaaataacgg gattttggat gatgtgcatt tatcgagaaa actaaaaacc
     1081 ctccgccgtc attcccgcga aagcgggaat ctagaccttt cggtgcggaa acttatcgga
     1141 taaaacggtt ttccctgcat cctggattcc cactttcgtg cgaatgacgg gatataggtt
     1201 tcactacgga cgtgttcaga ttaccgcctg cgcgggaagg acgggatttc aggttgctgt
     1261 ttttgatttt ctgtttttgt gggaataacg ggactttgga tgatgtgcat ttatcgggaa
     1321 aacaaaaaac cctccgccgt cattcccgcg aaagcgggaa tctagaattt taatgcggca
     1381 agaatttatc ggaaaaaact gaagtttaaa gacctagatt cccgcctgcg cgggaatgac
     1441 ggtttagaaa tcacctgaaa cgagaaaaag aaccgaaacc ggacaagtcg gattcccgca
     1501 cgggcgggaa ggacggaatt tcagattgtt gtttttgatt ttctgttttg tgggaataac
     1561 gggattttgg atgatgtgca tttatcggga aaactaaaaa ccctccgccg tcattcccgc
     1621 gaaagcggga atctagaatt ttaatgcggc aaaaatttat cggaaaaaac cgaagtttaa
     1681 agacctagat tcccgcctgc gcgggaatga cggtttagaa atcacccgaa acgagaaaaa
     1741 gaaccgaaac cgaacaagcc ggattaccgc ctgcgcggga atgacggctc aaaagttacg
     1801 aaacgaaaaa taaccgaaac caaacaagtc ggattcccgc gcgggcggga atgacgataa
     1861 gcgtaatttc aatctgccat attgtctttt cacttgcctg aaaacagatt tcagccacct
     1921 atccctgcat ccggcggctg atgtagtcgg caatcaaatc ccgcttttcc caaaaaaact
     1981 tctgcatttt ttgttcggaa tacagtttgc ggaccagagt gggcaaaccg ctgtggacga
     2041 tggcggcgat ttctttgttg cctttttctt cctgcattgc cgtctgaaaa tccatacccc
     2101 tgcctttcag atattctgca aggcggttgc ggatgtggga attgagttcg atggatttga
     2161 gcattttgcc tccgttttgc ggtaaggatt tattcgattt gtttatccgc ctgattttat
     2221 cacgtttccc aagcggtttg cctgtacaat accgcttttc ggcagggtgt gggcgatgat
     2281 gttttcttgg ttcaagctgt ttcacttgtt ttttgtcatt tcgtggtttg cagggctgtt
     2341 ttacctgccg aggattttcg tcaatatggc gatgattgat gtgccgcgcg gcaatcccga
     2401 gtatgtgcgt ctgtcgggca tggcggtgcg gctgtaccgt tttatgtcgc cgttgggctt
     2461 cggcgcggtc gtgttcggcg cggcgatacc gtttgccgcc ggctggtggg gcagcggctg
     2521 ggtacacgtc aaactgtgtt tgggcttgat gctcttggct taccagttgt attgcggcgt
     2581 gctgctgcgc cgttttcagg attacagcaa tgctttttca caccgctggt accgcgtgtt
     2641 caacgaaatc cccgtgctgc tgatggttgc cgcgctgtat ctggtcgtgt tcaaaccgtt
     2701 ttgacggagc atcttatgcc gatagaaatc gaacgccgtt ttttgattgg aaacgacaac
     2761 tggcggcaat acgccgacga accgctgctg ttgaggcagg gttatctgtc tgttgaaaaa
     2821 gaacgcaccg tccgtgtccg cattgccgga aaacgggcgt ggctgacgct gaaaggctat
     2881 atttcggaaa tcagccgcag tgagtttgaa tacgaaatcc cgcttgccga tgcggaaaag
     2941 ataatggaaa cgatgtgccc gtttaagatg gaaaaacggc gttatccggt cagatggaac
     3001 ggcagcctgt ttgaagtcga tgttttcctt ggagaaaacg cacctttggt cgtcgcagaa
     3061 atcgagctgc ccgacgaaaa tgccgatttt gaccgtccgg actggctggg gcgcgaaatc
     3121 actgccgacg gtatgtttac caatgcttat ttgagcaaac atccgttctc aagctggaaa
     3181 aatgccgtct gaagcggctt cagacggcat tctccgacaa ccatcgcaaa tccaaagaca
     3241 gcctctatat ccttatttct gctttctttg ttccattatt cccaataaat ccccataacc
     3301 taaaatctcg tcattcccgc gcaggcggga atccggttct ttaaacttcg gttttttccg
     3361 ataaattgct ttagcttttc gtttccggat tcccgctttt gcgggaatga cgatggaaga
     3421 ttgttgttat ttcggataaa ttaccctaac tttaaaatcc cggattccca tcttcggggg
     3481 aataacaata ttttctattt cagcccatca tttgcggggg attttataga gagtttatat
     3541 ttcaatatct caaataatgc acaaaagcat agcgcgtgcc tttgctgctg acgcggcgtt
     3601 ccgtccgccc tgcctctttc caatgcgtcc ggtctatttc ggggaaaaac gcatctcctt
     3661 ccacagacaa atccacttcg gttatccgca aatcggtcgc caatggcatc gcttgtccgt
     3721 atatctgcgc gccgcccata atgacggctt cttccgcgcc tgcgcacaat gccaatgccg
     3781 cctccaaact tgccgccgtt tccgcgcctg ccgcgcaata atccgcctgc cggctgatga
     3841 cgatgttcct ccgtccggac aggggtttga cgggcaggga ttcccacgtt ttccgcccca
     3901 taatgacggg tttgcccaag gtataggctt tgaaaaatgc gaaatcttcg gggatgtgcc
     3961 aaggcatagc attgcccgcc ccgatgcaca ggttttccgc acacgccgca attagggtta
     4021 ttttgagcat atttcctcca caaaaatgcc gtctgaacgc ttcagacggc attttatgac
     4081 attccgtgtc aaaaatctca accggcgcgc gccatacgtt ttttgtttgc acctgccaac
     4141 aatgccaaca gttcttcagt cgcgccccaa ccgatacacg catcggtaat gcttttgccg
     4201 tacacttccg gcttatcctg tctgccttcg accaaatggc tttccaccat cacgcccata
     4261 atattgctcc cgtcctgttc caattgggca gcaatatctt gtgccacttc catctgccgg
     4321 gtgtaatcct tgcggctgtt ggcgtggctg caatctatca tcagcttatc ggttacccct
     4381 gccgcacgca gttgttccgc cgcctcgctg acgtgtcccg catcataatt cggctctttt
     4441 ccgccgcgca aaatgacatg acagtcggga ttgccgccgg tatggacaat ggcggaatgt
     4501 ccggccttgg ttacagacaa gaaatgatgc gaatggctcg ccgcaccgat tgcgtcgatg
     4561 gcaatcttca aattgccgtc cgtaccgttt ttaaagccga cggggcagga cagcccgctt
     4621 gccaattcgc ggtgaacttg gctttcggtc gtccgcgcac cgattgcccc ccaagaaatc
     4681 aagtccgcat aatattgcgg cgtaatcata tccaaaaact cggtagaggc aggcataccc
     4741 atattgttca gcgacaacaa caggctgcgc gcctgacgca aaccgaaatt gatatcgaac
     4801 gtaccgtcca aatgcgggtc gttaatcaaa cctttccaac ccaccgtcgt cctcggcttc
     4861 tcgaaataaa cgcgcatcac aatcaaaagc tcgttttcat actgcttgcg gagtttcaac
     4921 aaacgctccg catattccaa cgccgctttc ggatcgtgaa tcgaacacgg cccgataata
     4981 accaacagcc gcttgtccct gccgtgaacc aaatcggaaa tttcctgacg ggtgcggtga
     5041 accaagcccg aagcctcttt ggaaatcggc agctcgtaaa gatgggcaat cggcggcaac
     5101 aactctttaa cttctttaat cttaatatcg tctgtggggt aatggtgtgt catcttgcat
     5161 ctttcctgat ggttttttat acgcggctta ggttacatcg tttaccttgc acaaacaagt
     5221 gcaacatatt actacaaagc tattttattt caaaataact gatatatctt ttttcataga
     5281 ttttaaattt catattttta atattttagg aattttattc caaagaaaaa gaaacagatt
     5341 gataaaaaca atacccgcca ttcattcgga caaaaccgca cggaacatcc gttttcccat
     5401 ttccacgaca atccggtaca gaacaccccg ctgccgattc cgcctgcaca tccggcaaaa
     5461 tttcatacgc aaggaaaatg ttgaaaacaa aaagaactaa ttgcttgaaa aaagatttaa
     5521 ttggaatgcg ggtatttccc acactttctc catttattca tcctcattat aggaagaaaa
     5581 acatgaaaaa accctgcttg cactctcact tgcagccgtg tccgccgtta cggtagccga
     5641 tattcctgag tctaacggca agggcaaagc catcatcggc gataaggaaa aacaccgtta
     5701 ccaagcatga aaaccttgtt ttgaaagtct atggcgaaat ccgccaagat ttgggggacg
     5761 gataatccct gaccccttgg cggatgcata tgcagatttg cgcggcaaaa ccaaagtgat
     5821 gacgacccag atgggtgctt cccgcgatgt cagcaaaagc gccaaaggtt ggagtgtcgg
     5881 tatcggtctg aatgtaggca aacagttgac cgacagcgtc ggtctcgagt ttgatccata
     5941 ctaccgtcac aaaacaatct gcaaaccccg tgagattgtc ttggacggtg acaaaaccaa
     6001 aatgggccgc tccaaatcca acgagtacgg cttccgcgta gccgcaacgt tctatagtca
     6061 attaaaatca aaataggaca gtagtgcatc gtcaaatcgg gcgtaatcag acaaaacggt
     6121 tcgcagatac cgcttaatat tcgcccacac tttctcaatc gggttgagct caggtgaata
     6181 agatgcaaga ggcaatacct tatgtcccaa tttttccgcc atttcccgta aga
//
LOCUS       DAXHKX010000119         6232 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHKX010000119 DAXHKX010000000
VERSION     DAXHKX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.119"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            109..1944
                     /gene="edd"
                     /locus_tag="ACFMWZ_001595"
     CDS             109..1944
                     /gene="edd"
                     /locus_tag="ACFMWZ_001595"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC5935535.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGRGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLSAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLIDEAE
                     WNAREVEHIDLGANQQGCGRELFAGFRSLTGGAETGAMSFGGEFA"
     gene            2052..2690
                     /locus_tag="ACFMWZ_001596"
     CDS             2052..2690
                     /locus_tag="ACFMWZ_001596"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC5935536.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            2705..3001
                     /locus_tag="ACFMWZ_001597"
     CDS             2705..3001
                     /locus_tag="ACFMWZ_001597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935537.1"
                     /translation="MPSEKPFPFQTAFCRLRAQSAYTAALIRHTAPKIPIRLPHNKNN
                     VQFIRQQPDSPYYAAEKMPSETHSDGIHLKKTTDSTPINPLPKPLSSLGFRRRV"
     gene            complement(2949..3989)
                     /locus_tag="ACFMWZ_001598"
     CDS             complement(2949..3989)
                     /locus_tag="ACFMWZ_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFC5935538.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKTQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPLPDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAAPVGMSALLTAQLYSPAAIIVCDMDENRLKLAKELGATHTINP
                     ASGEVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEMLMKAISSSSVDYTKMLTHHFKFSELEKAYDVFKHAA
                     ENQVMKVVLEAD"
     gene            4152..5192
                     /gene="mutY"
                     /locus_tag="ACFMWZ_001599"
     CDS             4152..5192
                     /gene="mutY"
                     /locus_tag="ACFMWZ_001599"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC5935539.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            5609..6169
                     /gene="sodC"
                     /locus_tag="ACFMWZ_001600"
     CDS             5609..6169
                     /gene="sodC"
                     /locus_tag="ACFMWZ_001600"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC5935540.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         1611 a         1757 c         1492 g         1372 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatactca ccgtctgaat acccgataca aaaatcagaa
       61 acgcacaaac aaatccccaa taccccccgt tccgacagga gaccgaccgt gaacactact
      121 cctatccact ccaaactcgc cgaaatcacc gggcgcatta ttgaacgcag ccgtccgacg
      181 cgtgaaaaat atctggcgaa gatccgcagt gccaaacaaa tgggacgctt agagcgcaac
      241 cagctcggtt gctccaactt ggcgcacggc tatgctgcca tgcctaaaag tatcaaaatc
      301 gaaatgcttc aggaaaccgt ccccaactta ggcatcatca ccgcctacaa cgacatggtt
      361 tccgcacacc agccgtttaa agacttccct gaccaaatca aagacgaagc gcagaaaaac
      421 ggcgcgaccg cccaagtcgc cggcggcaca cccgccatgt gcgacggcat cacgcaaggc
      481 tacgccggta tggaactgtc gctgttctcc cgcgacgtga ttgccatgag taccgccgtc
      541 gggctgtcgc atcaaatgtt tgacggcagc ctgtttatgg gcgtatgcga caaaatcgtt
      601 ccaggtttga tgataggcgc gctttcgttc ggtcatattc cgggtatctt cgtccccgca
      661 ggcccgatgt ccagtggtat cggcaacaaa gaaaaagccc gcacccgcca gcttttcgcc
      721 gaaggcaagg tcggacgcaa cgaacttttg aaaagcgaaa tgggttctta ccacagcccg
      781 ggcacctgca ctttctacgg cacggcaaac tccaaccaaa tgatgatgga aatgatgggc
      841 gtgcacctgc ctgccgccgc cttcgtccac ccttacaccg acctgcgcga agccctgacc
      901 cgctacgccg ccggacacct cgcgcgcggc atcaaaaacg gcacgattaa acctttgggc
      961 gaaatgttga ccgaaaaatc ctttatcaac gccttgattg gcctgatggc aaccggcggt
     1021 tcgaccaacc acaccatgca cctcgtcgct atggcacgcg ccgccggcgt gattttgaac
     1081 tgggacgact tcgacgaaat ttcctccatc atcccgctgc tcatccgcgt ttatccgaac
     1141 ggcaaggccg acgtgaatca ctttaccgca gcgggcggac tgcctttcgt tatccgcgaa
     1201 ttgctgaatg caggcctgtt gcacgacgat gtcgataccg tcgtcggacg cggtatgcgc
     1261 cactacacca aagagccttt cctcatcgac ggcaaactcg aatggcgcga agcccccgaa
     1321 accagcggca acgacgacat cctgcgcaaa gccgataacc cgttctcccc agacggcggt
     1381 ctgcgcctga tgaaaggcaa catcggtcgc ggcgtgatta aagtgtccgc cgtgcgcgaa
     1441 ggctgccgca tcatcgaagc acctgccatc gtgttcaacg accaacgcga agtattgtcc
     1501 gcatttgaac gcggcgagtt ggaacgcgat ttcgtctgcg tcgtccgcta ccaaggcccg
     1561 cgcgccaacg gtatgcccga attgcacaaa ctgaccccgc ctctgggcat cctgcaagac
     1621 cgcggcttca aagtggcgtt gctgaccgac ggccgtatgt ccggcgcgtc cggcaaagtg
     1681 cccgcatcca tccatatgac ccccgaagcc ctgatgggcg gcaacatcgc caaaatccgt
     1741 accggcgacc tgatccgctt cgattccgtg accggcgaac tcaacgtcct gattgacgaa
     1801 gccgaatgga atgcccgcga agtcgaacac atcgacttgg gcgcgaacca acaaggctgc
     1861 ggccgcgaac tcttcgccgg cttccgcagt ttgaccggcg gcgcggaaac cggcgcgatg
     1921 agcttcggcg gcgaatttgc ctgatgcgcg tttcagacgg ccttcgtgaa tagaaggtcg
     1981 tctgaaaaat taaatcccca tcccgtttga ccgcagcaaa aacaacaccc cattccaccc
     2041 ggagaaccga aatgtccaaa ctgacccccc gcgaaatttt gaccgccggc gcagttgtgc
     2101 cggtaatggc gattgacgac ttaagcaccg ccgtcgattt gtcccacgcc cttgtcgaag
     2161 gcggcatccc caccctcgaa atcaccctgc gcacccctgt cggcctcgat gccatccgcc
     2221 tgattgccaa agaagtgccc aacgccatcg tcggcgctgg tacggttacc aaccctgaac
     2281 agctcaaagc cgtcgaagac gcaggcgcgg ttttcgccat cagcccgggt ttgcacgaat
     2341 ccctcgccaa agccggccac aacagcggca tccccctgat tcccggtgtt gccaccccgg
     2401 gcgaaatcca actggctttg gaacacggca tcgacaccct caaactcttc cccgccgaag
     2461 tcgtcggcgg caaagccatg ctcaaagccc tttacggccc ttacgccgat gttcgcttct
     2521 gcccaaccgg cggcatcagc ctggcaactg cccccgaata cctggcactg cccaacgtcc
     2581 tgtgcgtcgg cggctcttgg ctgacaccga aagaagccgt gaaaaacaaa gactgggaca
     2641 ccatcacccg cctcgccaaa gaagcggcgg cattgaaacc caaagcctga accgcatcgt
     2701 aaaaatgccg tctgaaaaac ctttcccgtt tcagacggca ttttgtcgat tgagggcaca
     2761 gtcggcatac acggcagcac tgatcagaca taccgcccct aaaataccca tccgccttcc
     2821 gcataataaa aataacgttc agttcattcg acagcagccg gacagcccat actacgcggc
     2881 tgaaaaaatg ccgtctgaaa cgcattcaga cggcatccac ttaaaaaaaa caactgattc
     2941 aacgccgatt aatccgcttc caaaaccact ttcatcactt ggttttcggc ggcgtgtttg
     3001 aacacgtcgt aggctttttc caattcactg aatttgaaat gatgggtcag cattttggtg
     3061 taatcgacgg agctgctgga aatcgccttc atcagcattt cggtggtgtt ggcattgacc
     3121 agaccggtag taatggcgag gtttttaatc cagagttttt ccagtttgaa atcaacggat
     3181 tgaccatgta caccaaccac agcgatatgg ccgccgggtt tcacaatgtc ttggcacata
     3241 ttccatgtag cagggatacc gacggcttcg atggcgcaat ccacgccgtc ttcgccgacg
     3301 atggcaaaga cttgtttgga tacttcgccg gaagcagggt taatggtatg ggtcgcaccc
     3361 aattctttcg ccagtttcaa acggttttcg tccatatcgc aaacgatgat ggcggcggga
     3421 ctgtacagtt gggcggtcaa cagggcggac ataccgacag gagcggcacc tgcgatgaat
     3481 acggtgtcgc cgggtttgac atcgccgtat tgcacgccga tttcgtgggc ggtcggcaaa
     3541 gcgtcgctca acaacagggc gatttcttcg ttgacattat cgggcagcgg aacgaggctg
     3601 ttgtcggcat aaggcgtacg gacgtattcg gcctgagtac cgtcaatcat gtaacccaaa
     3661 atccaaccgc cgttacggca gtgtgaatag agttgggttt tgcagttgtc gcaagtgcaa
     3721 catttgctga cgcatgaaat aatgacttta tcgccgactt tgatgttttt tacagcctcg
     3781 ccgacttctt ctacaatacc gatgccctca tgaccgagaa tacggccgtc ggcaacttcg
     3841 gggtttttgc ctttccaaat acccaagtcg gtaccgcaaa tcgtggtttt gacgattttc
     3901 accaccgcat cggtcggatc gataatctgc ggacggggtt tttcttcaaa acggatgtcg
     3961 tttgcgccgt gataaaccat tgctttcatg ctgatactcc ttgcttgtta ataaataatt
     4021 tcaataccgc aataaagttt ccttatatga gttatatgcc cctacaaaaa ataagtcaat
     4081 aagaattatt ttcacaatgt tatacaataa catactgttt taaatataaa taaaaccacc
     4141 gattgatatt aatgaacaca cccatcccct tctccgaacg gctcatccgc tggcaaaaac
     4201 aacacggtcg ccaccacctc ccttggcagg tcaaaaaccc ttattgcgtc tggctttccg
     4261 aaatcatgct ccagcaaacg caagtcgcca ccgtgttgga ctactatccg cgcttcttag
     4321 aaaaattccc gaccgttcag acgcttgccg ccgcgccgca agacgaagtg ttgtcgttgt
     4381 gggcgggctt gggctattac agccgcgcgc gcaacctgca caaagccgcg caacaagtcg
     4441 tcaggcaatt cggcggcacg tttccgtcgg agcgcaaaga cttggaaacc ctctgcggcg
     4501 taggcagaag caccgccgcc gccatttgcg ccttctcctt caaccgccgc gaaaccattt
     4561 tggacggcaa cgtcaaacgc gtactctgcc gcgtgttcgc ccgcgacggc aatccgcagg
     4621 acaaaaaatt tgaaaactcg ctctggacac ttgccgaaag cctgctgccg tctgaaaacg
     4681 ccgatatgcc tgcctataca caaggtttga tggatttggg cgcgaccgtg tgcaaacgga
     4741 cgaaaccctt gtgccaccaa tgcccgatgg cggacatctg cgaagcaaaa aagcaaaacc
     4801 gcaccgccga gctgccgcgc aaaaaaaccg ccgccgaagt accgaccctg ccgctttact
     4861 ggctgattgt ccgcaaccgg gacggcgcga ttttgctgga aaaacgcccc gccaaaggca
     4921 tttggggcgg gctgtattgc gtgccgtgtt ttgaaagttt gaacgggctt tccgactttg
     4981 ccgccaaatt ctccctgacc atggcagata tggacgaaca aaccgccctg acccaccgcc
     5041 tgacgcaccg gctgctattg attacgccct ttgaagcaca aatgccgtct gaaagccctt
     5101 cagacggcat ttggataaag ccggcgcatt tgaaagatta cggcctgccc aagcctttgg
     5161 aaatttattt aaacggtaat aggttagaat aaacaaaata aacccattga actgttgttt
     5221 gcaggtatcg cagcaagaac aaccgatgaa tttgggtcgt attttaggcg gcgggataat
     5281 gttcaaatgg gacatttgga acggaagaag tcggcaattt aaaaaggatt taaaaagcaa
     5341 agaaggtcaa aaacatgaac acaaacttaa atgacaaaga caaagccatg gataccgcaa
     5401 tcaggtttca gaaaaggatg aggattccga aatttttctt tttaattctc taaatcacaa
     5461 tggttttggc atttatccaa gacgtgataa cgggttctaa ttttctgcaa ataacaatta
     5521 atgtaaaatt ttcgtaaaaa tttatcggct tttaaaacaa aattgactaa aatagtcgcg
     5581 agtttttact gcaataaagg agattgcaat gaatatgaaa accttattag cactagcggt
     5641 tagtgcagta tgttcagtta gtgttgcgca agcacacgag cataatacga tacctaaagg
     5701 tgcttctatt gaagtgaaag tgcaacaact tgatccagta aacggtaaca aagatgtggg
     5761 tacagtgact attactgaat ctaactatgg tcttgtgttt acccctgatt tacaaggatt
     5821 aagcgaaggc ttacatggtt tccacatcca tgaaaaccca agctgtgagc caaaagaaaa
     5881 agaaggtaaa ttgacagctg gtttaggcgc aggcggtcac tgggatccta aaggtgcaaa
     5941 acaacatggt tacccatggc aagatgatgc acacttaggt gatttacctg cattaactgt
     6001 attgcatgat ggcacagcaa caaatcctgt tttagcacca cgtcttaaac atttagatga
     6061 tgttcgcggt cactctatta tgatccacac gggtggtgat aatcactccg atcatccagc
     6121 tccacttggc ggtggcggcc cacgtatggc atgtggcgtg attaaataat tcgattgttc
     6181 gaaacgaaaa gtgcggtgaa ttttgaccgc acttttttgc tagatattta gc
//
LOCUS       DAXHKX010000120         6136 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHKX010000120 DAXHKX010000000
VERSION     DAXHKX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.120"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            57..1610
                     /locus_tag="ACFMWZ_001601"
     CDS             57..1610
                     /locus_tag="ACFMWZ_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC5935541.1"
                     /translation="MNRTYANFYGMLAAACRKNGNGTAVFEGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGKSRPVGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1700..3726)
                     /locus_tag="ACFMWZ_001602"
                     /pseudo
     CDS             complement(1700..3726)
                     /locus_tag="ACFMWZ_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            4015..6021
                     /locus_tag="ACFMWZ_001603"
     CDS             4015..6021
                     /locus_tag="ACFMWZ_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC5935542.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTTAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         1570 a         1862 c         1433 g         1271 t
ORIGIN      
        1 cggcatattg ttttcaaatg agggcgttcg ccgtccgcaa ccataaagga agtttcatga
       61 accggactta tgccaatttc tacggaatgc tcgccgccgc ctgccgcaaa aacggcaacg
      121 gcacggcagt gttcgaaggc aaggaaaaaa ccgcctaccg cgcactcaag caggaagccg
      181 aagccgttgc ggcgtatctg caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg
      241 cggtttccaa ttccacagaa tttattaccg cctatttcgc cgtctccgcc atcggcgcgg
      301 tcgccgtacc gatgaacaca tttttgaaaa acagcgaata cgcgtatatc ctgaacgact
      361 gcaaggcgcg cttcctgttc gcctcggcgg gcctgtcaaa agaattggcg ggcttgaagg
      421 cgcaaacgcc cgtcgaaaaa atcatttgga cgggcaaaag ccggccggtc ggcgaaacgg
      481 cggaaggcga tgcctttttt gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc
      541 gacaaccccg gataaatgat ttggcacaca tcatctacac ctccggcacg acggggcatc
      601 ccaaaggcgc gctaatcagt tacgccaacc tgtttgccaa cctgaacggc atcgaacgca
      661 tctttaaaat ttccaaacgc gaccgcttta tcgttttcct gccgatgttc cacagcttca
      721 cgctgacggc tatggtgctg ctgccgattt atatggcgtg ttcgattatt ttggtcaaat
      781 ccgtttttcc gttttccaac gttttgaaac agacactgct caaacgcgcg accgtgtttt
      841 tgggcgtacc cgcgatttac accgcgatga gcaaggcgaa aatcccttgg tatttcagat
      901 ggttcaaccg catccgcctg tttatcagcg gcggtgcgcc tttggcggaa caaaccatcc
      961 tcgatttcaa agccaagttc ccccgcgcca aattgctgga aggctacgga ctgagcgaag
     1021 cctcacccgt cgtcgccgtc aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc
     1081 ccctgcccgg tttggaagtc aaagccgtcg atgaagaact ggtcgaagtg ccgcgcggcg
     1141 aagtgggcga actgattgtc aggggcggtt cggtgatgcg gggctacctc aatatgcctg
     1201 ccgccaccga tgaaaccatc gtcaacggct ggttgaaaac gggcgatttc gttaccatag
     1261 acgaagacgg ctttatcttt atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc
     1321 aaaatgtcta tccgcgcgag attgaagaag aaatctacaa actcgatgcc gtcgaagccg
     1381 ccgccgtcat cggcgtgaaa gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat
     1441 tgaaggaagg tatggatttg ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg
     1501 caaatttcaa aatccccaaa caaatccact ttaaagacgg gctgccgcgc aacgctacgg
     1561 gcaaggtatt gaaacgggtg ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc
     1621 gtccgaagcc ccgtccggca aaaaaaatgc ggtgaatctg cttcaccgca ttttttggca
     1681 aacgccggag ataaggcact tactctgcca attccacctg ctcgtgcgcc atcagttcct
     1741 gaccgacatc atccaacaac atcaaataac gttcgtagtt tttcaaaatg tcggcaatca
     1801 gctcgtcctt gttcatatac tgtacatcgt acccgacgcg cccgtcgaaa aaataagcgt
     1861 agggtttgta agttgtctga tgccggatat gcggcagctt gccgtcgtta atcaactggt
     1921 cggatacatc ctgcccgaca gacttaatcc cgtacataaa atcgcgcatc gtctctttcc
     1981 gaatgacgaa ctcgattgcg ggctcgtcct gatgaaacat cttatcgacc cggacgctca
     2041 agccgtattc ttccgaaagc tcccgttgca actcgtgcat agcgggcgat gcggtatgtt
     2101 tgaggaattt taaaatatcc tgctcctgcg tctggctcat tatctgcacc agccgttctt
     2161 tccacttgcc gcccgtccaa aatacactgg tagggttaac ccgggtctca aaatatttct
     2221 tatccgcact caagcctttc cacaggctga aacacattat cagcatcagc agggcaaacg
     2281 gcagggaaac aatcagggtc atagactgca ggttgccgag tccgcccgag cgcatcagca
     2341 aaacggcaac ggcagacatc agcacgcccc acataaccgc ctgccaccgt ggcgcgctca
     2401 agcctttgtc ccgagaggta atattgttca ggacataaat cccggaatcg gcagaagtta
     2461 caaaaaacag agaaatgacc agcaggctga cgatgctcgt caattcgggc aggggaggta
     2521 attaaagaat ttaaaaagca gcgtttccgg agaggaggtc atcttttcga gcattccccc
     2581 cgcaaccccg tcattcagcc aaatcgccgt attgccgaag acggtaaacc acaaaacgcc
     2641 gaacaggccg gggatgagca aaaccccgaa gacaaactcg cggatggtgc gcccctttga
     2701 aatgcgcgcg ataaacaaac ccacaaacgg cgcccaagaa caccaccacg cccaataaag
     2761 caccgtccaa gattcaaacc acggcttgtg ttcccgttcg tacgcataag ttttaaaact
     2821 gaggcgcacc agatttccga ggtagttccc tatgttgtcg ccgaatgccg acaacaggta
     2881 aacagtggga cccgccgcca aaacaaaaaa cagcagcaaa aacgcaaggc tcaggttcaa
     2941 ctcgctcaac accttcacgc ccttccccac gccggatatt gccgaaacga cggcgaggga
     3001 catgacggcg gcgataatca aaacctgcac gctgaagctg ttttcggcaa tccagcccat
     3061 ttcctgcaat ccggcgccca gttgcgaagc cccgaacccc aatgtggtga tgatgccgaa
     3121 aaaagtagca agcaacgcca taatatcaat ggcatcgccg aaccttccgg aaattttttc
     3181 tttcaacagg gggtaaaaac aagaacgcag ggcaagcggc agcttgtagc ggaaaccgaa
     3241 ataagccaaa gccaatgcaa tcgtaccgta caccgaccaa gcgtgaacgc cccaatggaa
     3301 caccgtgtgc agcaatgcct gctgctgcct gtgttccggc gtgccggccg taatgtccga
     3361 aaaataatgc atcaacggct ctgccacgcc gaaaaacatc agacccacgc ccatcccggc
     3421 cgcaaacagc atcgccagcc acgacaggaa gccgaattcc ggcacatctt catcccgtcc
     3481 gagcctgatg tttcccaaac tgctgaccga gagtatcagc aggaaaccca gaaaaatgga
     3541 aaacgttaaa acataaaacc agctgaactc ggtaaaaatg acttcttttg cccgatcgag
     3601 ccacatctgc acctgatccg gcacggttaa aaccaatacc actaaaacac acacaaaaaa
     3661 caaagtcgtc aaaataacca tcagattaaa tgacgttcgg cgttctataa attcagacag
     3721 ggacaaacct tctcactcct ttgttaaaaa cagacaaacc cggtcatcgg gcaaagcggt
     3781 caaaacctgc cggtaaaata ccggcttccg gatgacgaaa tgcacaaacc ggccgaaatt
     3841 gttataatcg aaaaaattaa aaatcaatac ggattattct atcagattga tttatcgata
     3901 tttattatat cattaatata gtttgatttc aaaccggcgg aaaatgcgcc gccttccgca
     3961 ggcgcgccct ccccgctatc ggggcgttta acgccggatt gccgatgcgg tacaatggct
     4021 gatatgaaga aaattacccc tcaaaacctg cgccccctgc tttcggaaag cttgggacat
     4081 accgattttg tcaacgtcct caacgcactg attaaatttt tgcgccgtgg cggcaaaaaa
     4141 tgtgcggggg aacgtttcga cctgattatc gacacattca aacaagacag ggaattactg
     4201 tcccgcttca gccggtgttt ttacatctgg ctcgcgcaaa tacacattta tccggcactc
     4261 atcaaactcg gcatcttctc gcgccacagc tttgcccggg aaatgggcat acgcatctac
     4321 gaacgcttca gcccgtcata taaagatttt gccaacttgg gcgaagtctt cctttatctt
     4381 ttccattccg aaaacgacga caaatggctg caaacgctca atatccgcca atggctggtt
     4441 ttatacgaac tcatccggag ccacgccgag ccgtccaaat tgcagacggc gggcatccgc
     4501 cttgccgatg cgcgtttgcg cgccatcgaa atgctgtctg tctggacggc atccgaagcc
     4561 atcgaacccg acctcatccg catcgccccg cgcctgctgg aagccgattc ttccttcgtc
     4621 gccctccaac gcgaaaccgc caaactggtc gaacactacc gcaacggcac cacgccttac
     4681 gacaccgccc acctcgaagt gatgttcgac caatgtttca gccagattga ctatttgcgc
     4741 cgcaaaggga cgggcgccgg ctccggttcg tcggtcaaag tcgcccacct gctcgaacgg
     4801 ctccggcaga ccgtagaccg tctaaagctg ctcaccgaca tccaaaccgg tgccggcaac
     4861 agcaaccgcc tgaccatcgc cctgatgaac tccctcatct acgcggcggt cgaacaatac
     4921 agcacccgcc acctgcgccg cagcagcatc cgtatgctcg cccgcagcat taccgaaaac
     4981 aaaagccacc acggcgaaca ctacatcacc cgcaaccgca aagaatattt caaaatgttc
     5041 tactcggcgg caggcggcgg catcatcatc gccctgatgg cgctgctcaa aatccgcatc
     5101 ggcacactcg gcctcagccc cttcctcact tccttgtcgg cggggttcaa ctacggcatc
     5161 ggctttatga tcatccatat gctgcactgc accgtcgcca ccaagcagcc cgcgatgact
     5221 gccgccagct ttgccgaaca ggtcgatctc aacgaaggcg gcaaagcggt ggacaacaaa
     5281 ctcgccaagc tcctcatcga cgtatgccgc tcccaaagcg tcgccgtctt cggcaacgtt
     5341 tccatcgcca tccttttggc gtgcgccata tcgttcggct atgcccatct gtaccggctg
     5401 cccatactcg atgcccacac cactgcctac cagttcaaat ccatagacat catcgcttac
     5461 ccgacgctgt ggtatgccgc cattgcaggt ctgtggctgt tctgctccgg catcatcgca
     5521 ggttttttcg acaaccgcgc cgactacctc aacctgcgcc aacgcctgcc cttcaacccc
     5581 ttgctgcgta aaatcatgcg ccccgggcct cgccgcgtcc tcgccgccta catccacaaa
     5641 cactacggct cgctggcagg caacttcatc ttcgggatgc tcttaggtat gaccggctat
     5701 ttcggacacc tcctcgggct gccgctggac atccgccacg tcgccttttc ctccgccaac
     5761 ctcggctatg ccgccgtcag cggcaacgtc ggtttgggca cgttcgtact cggcattttc
     5821 agcgtcctcg ccatcggctt ggtcaacctc tgcgtcagtt tcagcctcgc cctcttcgtc
     5881 gccctgcgct cgcgcggcac gaaaatcggc agtatccgca atctgattaa aagtttttgg
     5941 aatcagatta aaagcaatcc ctgcatactt ttcctcccgc ccgccaaaga acagggacat
     6001 cctccttcgg acaagccttg accggcaatg ccgtctgaag cgggattcgc cccgaatacc
     6061 gccctgatgc gggaaatccc cataaaagga tgcaaaaatg ccgtccgaac cgaaacgtgg
     6121 ttcagacggc atttta
//
LOCUS       DAXHKX010000121         6118 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHKX010000121 DAXHKX010000000
VERSION     DAXHKX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.121"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="ACFMWZ_001604"
     CDS             complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="ACFMWZ_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC5935543.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2288..2560)
                     /locus_tag="ACFMWZ_001605"
     CDS             complement(2288..2560)
                     /locus_tag="ACFMWZ_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935544.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2746..3432
                     /locus_tag="ACFMWZ_001606"
     CDS             2746..3432
                     /locus_tag="ACFMWZ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC5935545.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRNTKNLSVISVKGDSMEGVLNDGDSILVN
                     HSENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNHPADDVEII
                     GRVEWFGRTI"
     gene            3564..3902
                     /gene="erpA"
                     /locus_tag="ACFMWZ_001607"
     CDS             3564..3902
                     /gene="erpA"
                     /locus_tag="ACFMWZ_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC5935546.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3919..4065
                     /locus_tag="ACFMWZ_001608"
     CDS             3919..4065
                     /locus_tag="ACFMWZ_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935547.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4062..4526
                     /locus_tag="ACFMWZ_001609"
     CDS             4062..4526
                     /locus_tag="ACFMWZ_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC5935548.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPKVDCGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWEKRAELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     GDGEIPEYLELHSEREDDISAPSQAEPPPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            4561..6072
                     /gene="ubiB"
                     /locus_tag="ACFMWZ_001610"
     CDS             4561..6072
                     /gene="ubiB"
                     /locus_tag="ACFMWZ_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC5935549.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAVWVERLFADGKRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1382 a         1363 c         1639 g         1734 t
ORIGIN      
        1 aaaaatttcc agagaaccga tacaacagtt ggaacttggg tttgggaata ttacggtaga
       61 tgaacttgga acctctgtta tgctatggtc ttttatctca attgaaaaaa gcgcgaatca
      121 aacggttcgc gcttttttca gacggcatca attatttttt gtcgtctttt acttcttcaa
      181 agtcggcatc tacgacatca tcgtctttct ttgcagaagc attggcttgt tcgctttcgc
      241 ctgcttgggc ttcagcttgt gcttgagcgt aaaccatttc gcccagtttt tggctggctg
      301 tgcccagcgc ctcggctttg gcatcgatgg cggctttgtc gtcgcctttc acggcttctt
      361 cggcttcttt cagcgcggct tcgatttttt ctttttcggc agcgtcgagt ttgtcgccgt
      421 agtccgccaa agattttttc acggagtgaa tcagggcttc ggcttggttg cgggaagcga
      481 ccaattcagt cagcttttta tcttcctcag cattggcttc ggcatctttc accatgcgtt
      541 cgatttcttc ttcgctcaaa cctgaagaac cttggatggt aatgttggct gctttaccgg
      601 tgcctttgtc tttggcggaa acgtgcagga tgccgttggc gtcgatgtcg aaggtgactt
      661 cgatttgcgg cataccgcgc ggtgcaggtg cgatgtcgcc caagttgaac tgacccaaag
      721 atttgttggc agaagcgcgt tcgcgttcgc cttgcagtac gtggatggtt actgcgcttt
      781 ggttgtcttc ggcggtagag aacacttgcg acgctttagt cgggatagtt gtgttctttt
      841 gaatcagctt ggtcatcacg ccgcccatgg tttcgatacc caaggacaga ggggttacgt
      901 ccagcagcaa tacgtcgctg cggccgccgc ttaacacttc gccttgaatc gctgcgccta
      961 cggcgacagc ttcgtcaggg ttcacgtctt tgcgcggctc tttgccgaag aagtctttaa
     1021 cggcttcttg tactttcggc atacggcttt gaccaccgac caaaatcacg tcgtcgatgt
     1081 cgccagtgct cagaccggca tctttcaatg cggtgcggca aggctcgata gagcgggcaa
     1141 tcaggtcttc aaccaggctt tcgaatttgg cgcgggtaat tttcatcgcc aagtgtttcg
     1201 ggccggttgc gtccatggtg atgtacggca ggttaatttc ggtttgctgg ccgctggaca
     1261 attcgatttt ggctttttcg gcagcttctt tcaggcgttg tagagccatt acgtcttgtt
     1321 tcaaatcaat gccttgttct tttttgaact cggcgatgat gtggtcgatc aagcgttggt
     1381 cgaagtcttc accgcccaag aaggtatcgc cgttggttgc caatacttcg aattgtttgt
     1441 cgccgtcgag gttggcgatt tcgatgatgg aaatatcaaa agtaccgccg cccaagtcat
     1501 atacggctac tttgcggtct ttgttgtcgc ctttgtccat accgaatgcc aaagcagctg
     1561 cagtcggctc gttgatgatg cgtttcacgt ccaaaccggc gatacggcct gcgtctttgg
     1621 tagcttgacg ttggctgtcg ttgaagtagg cagggacggt aatcacggct tcggttactt
     1681 tttcgcccaa gtaagcttcg gcggcttctt tcattttacg caggacttct gcggaaattt
     1741 gaggaggaga cagctctttg ccttgtgctt ttacccatgc gtcgccgttg ttggctttga
     1801 tgatttcgaa aggcatagat tcgatgtcgc gttggacttc tttgtcttca aatttgtggc
     1861 cgatcaggcg tttggcggcg taaatggtgt ttttagcgtt ggttacggct tggcgtttgg
     1921 caggcgcacc gacgaggatt tcgccgccgt ccaaataagc gataacggac ggcgtggtgc
     1981 gtgcgccttc tgcgttttcg atcactttgg tttgaccgtt ttcggaaatg gccaaacaag
     2041 agttggttgt acctaagtcg ataccgatta cttttgccat gtggataatc ctatttgatt
     2101 ttgcttattt tgagaaatat gttggaacat tttgtcccga tgggctgtaa atagggcggg
     2161 cggcgggctg tttcaagcta cagcatggct ataagtatat aactttatga atatattggt
     2221 tttatatttg atttaataca tttggctcca atgcattcaa gcataatgtt tcaaatggca
     2281 ggcaggttta ttcatagacg atgccggcga gcatttcctg ttcgttcaag ttgccgtact
     2341 ctttttccca gtcgtgtgaa gactcgatga tgtcgcattc tttggaaagg gagacttgtt
     2401 ctgcatccat atctttggcg ttcagtatgt tgaattgttc gcatagggat gcggataaag
     2461 tgatgtcagg ctgtttggct tcagaacggt tttcttggaa ggcaaagcag aatgcggtaa
     2521 atgccgcagc atagataaga tatttgccgg ttttcttcat tttctatcct ttttctgtca
     2581 actcaggatt aaacctatgg aaaatcttaa aaattatgta ttgagtaaga aaaatcataa
     2641 tttaaattta gtttatcata gttgttccgt ttttgggata gctaaggtaa aatatatttc
     2701 atgtttactt tagatgattg aatgaagggg agcggaagga tatttatgga aacctttaaa
     2761 gacagactgg tttttttatg gaaaagcgaa gcgaagcagg caaaaatcgc atccgatatt
     2821 gaaatgacga ttgcgggctt cagcaggata tggaatgaag gcggtctgcc gaagtctgaa
     2881 acattaaaaa aaatcaagca gttgaagggg tgtagtatcg attggctgct gaccggggag
     2941 ggtaatccgt ttccggatga agccccaaaa aaatcccttg cttacgatac cttgggaaat
     3001 gaagtcgata cggacgagtt tgtctttgtg ccgagatatg atattcgggc ggctgcggga
     3061 tacgggcagt ttgtcgatca tgaggaaccg gtatttacaa tggcgttcag acggcattgg
     3121 attgagaatt atgttacccg caatacgaaa aacctgtctg taatttccgt caagggggat
     3181 tcgatggagg gggttttgaa tgacggcgat tcgattttgg tcaaccatag cgagaatacg
     3241 ccgagggacg gactgtatgt gttgcggatt aatgaaaatc tgctggttaa acgtttacag
     3301 attgtaccgg gcgggataat caacgttatt tccgccaacg aggcttaccc tgcttttgaa
     3361 attaatttga atcatcctgc cgatgatgtg gaaatcatcg ggcgggtcga gtggttcggc
     3421 aggacgattt gagtttgggg cttgaaatcg ggagcggtca aacttatctg taaggcagtt
     3481 ccttttcagg gaacaagccc ccgcttgcct ttgaaggggg tttgagagag aatgcagaaa
     3541 atattatatt aaggaataac accatgtcgg atgaaagccc tattattttt actgacagct
     3601 gctgtgccaa agttgccgat ttgattgccg aagaaaacaa tcccgatttg aaattgcggg
     3661 tttttgtcaa tggcggcggc tgttcgggtt tccagtacgg atttactttt gatgaaatca
     3721 aaaacgacga cgattttgaa attgagaaaa acggtttggt ctttttggtc gatccgatga
     3781 gctatcaata tctggtcggc gcggaaatcg actatacgga aagtttgcag ggttcgcaat
     3841 tcgtcatccg caatccgaat gcggaaacaa cctgcggttg cggatcgtcg ttttccgtat
     3901 gaccgcttgg tttgtgtgat gccgtctgaa cgttcagacg gcatttttac ttttggaaaa
     3961 tatattatcg ggatgaattc acatataatc cgattgtttg aagatgaatc gggtttcccg
     4021 aaaggaacgg gcggaacggt atcaggcgta tttgttccct tatgattgag atgagtaaag
     4081 attaccgaaa cgatttgtac gatgtatatg tttcctaccc gcccaaagtg gattgcgggc
     4141 ttatccggga gtgtctcagg gagaatctcg gcgaagaaaa ggcggaagga ttgattgagt
     4201 ccctcgattc caaaccgcaa gtgctggttg aggaaaaatg tacctgggaa aagcgggcag
     4261 agttgcacga ttatttcagc tatttgggtt tggatattat tactcggaga tatatggagt
     4321 tagaaacggt cgtgccgccg gaggaagggg agggcgaagg agaaggcggg gatggggaaa
     4381 tacccgaata tcttgaactt catagcgaac gggaagatga tatttccgca ccttcgcaag
     4441 ccgaaccgcc gccccgcaat atcaaaccgc tggttttcgg gctgctgata gcctttttgg
     4501 gctatctgct cggtaagatt ttttgattgt ccgataaatg ctgtattcgg gattttatat
     4561 atgaaatggt tgaaacgcct gacggttatt gtcgggactt tttaccgcta tcggctggca
     4621 ggtctgtgcg cttcgctgat gggtagcggt tggatatgcg ctctgctgaa aatgatgccg
     4681 cagtcgtcca aattgaaaaa cgaaccgcct gccgtccgtc tgcgccttgc cttggaaagc
     4741 ctggggccga ttttcatcaa gttcgggcag gttttgtcca cacgccctga tttgattccg
     4801 cacgattacg ccgtcgaact ggcaaagctg caagacaaag tgccgccttt tgacgcgcgg
     4861 ctttcgcgtg aacaaatcga aaaatcgttg ggtcagtcca tcgaaaagct gtatgcggaa
     4921 tttgaaaccg agcccgtcgc cagcgcgtcc atcgcccaag tacacaaagc ccgcctgcat
     4981 tcgggcgaac gagtggctgt caaagttttg cgccccaacc ttttgcccct tatcgaacag
     5041 gatttgtcgc tgatgcgttt cggcgcggtt tgggtggagc gtctgtttgc cgacggcaag
     5101 cgtctgaagc cgctcgaagt ggtggcggag ttcgacaaat acctgcacga cgagttggat
     5161 ttgatgcgcg aagccgccaa tgccggacag ctcgggcgca atttccaaaa cagcaatatg
     5221 ctgattgtgc ccaaggtgtt ttacgactac tgcaccagcg acgtgctgac aatcgaatgg
     5281 atggacggca cgccggttgc cgacatcgcc aaactcaaag cagacggcat cgatttgcac
     5341 aaactcgccg attacggcgt ggaaatcttt ttcacgcagg tcttccgcga cggctttttc
     5401 cacgcggata tgcaccccgg caatattttg gttgccgccg acaaccgcta catcgccctc
     5461 gatttcggca tcgtcggcac gctgaccgat tacgacaaac gttatctcgc catcaatttc
     5521 ctcgcctttt tcaaccgcga ttaccggcgc gtcgccaccg cccacatcga atcgggctgg
     5581 gtgcctgccg acacgcgtgc ggaagaattg gaagccgccg tccgcgccgt gtgcgaaccg
     5641 gtgttcaaca aaccgatttc gcagatttcc ttcggcttgg tgctgatgcg cctgtttgaa
     5701 gtcagccgcc gcttcaatgt cgaaatccaa ccgcagcttg tgctgctgca aaaaacgctg
     5761 ctcaacatcg aaggcttggg ccgccagctc gatcccgatt tggacttgtg gaaaaccgcc
     5821 aaaccgtttt tggtgaaatg gatgaacggg caggtcggcc ctaaagccct ttggcgcaac
     5881 ctcaaaaacg aagcccccga ctgggcgcaa atcatccctt cattgccgcg caaaatcagt
     5941 gcgttgattg atgaaaaccg ccagcaggaa atgcgtgatg cctatatcca tttggtcaaa
     6001 gtgcagcagc ggcaaagcct gtggctggct gtgattgcgg ttgttttgct gctgattttg
     6061 cttttgaaat aggctttgtc cgaatcatcg cccgactccg cccgtttata aggaaatc
//
LOCUS       DAXHKX010000122         6055 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHKX010000122 DAXHKX010000000
VERSION     DAXHKX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.122"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..801
                     /locus_tag="ACFMWZ_001611"
     CDS             <1..801
                     /locus_tag="ACFMWZ_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC5935550.1"
                     /translation="GAIGAVVGEIVGEALVKNTDFSGMTASEIEKAKANITAYAKLVA
                     GATVGVTGGNVDVAANASETAVKNNALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREA
                     AIDFGVDAAAAAVPFVPAGATKISRGGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYD
                     KVNKVWTTPAGLDYGLDAKHGNRIKHVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAW
                     RMKGNPLPNDSSVYLVDMKKPIGTKGETKVRIVVQKGTNKIISAYPQK"
     gene            820..1083
                     /locus_tag="ACFMWZ_001612"
     CDS             820..1083
                     /locus_tag="ACFMWZ_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935551.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            1190..3115
                     /locus_tag="ACFMWZ_001613"
                     /pseudo
     CDS             1190..3115
                     /locus_tag="ACFMWZ_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            3124..3546
                     /locus_tag="ACFMWZ_001614"
     CDS             3124..3546
                     /locus_tag="ACFMWZ_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935552.1"
                     /translation="MKYMINFLKKTFELMSWVLVILIIGTFYDYYQIRQYAELEKKSI
                     SNILLYAQKEKFRLESKDKYMRGGYTKYEFIFSEYSNTTFLNFINDLKKDNYLPLDGY
                     GHGFLCRKGESISINIYPEINKFILVWGYPENLCADSN"
     gene            3583..3984
                     /locus_tag="ACFMWZ_001615"
     CDS             3583..3984
                     /locus_tag="ACFMWZ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC5935553.1"
                     /translation="MRKLNNHDVHKRYQDRLEEDVEFTINYELPLSCLWSTIKDFSSD
                     FEEKTEAFFILFKELLRRGHLKLQRDGQIIGHTPEEWEQIFREVWSEYEIEPNPLPGY
                     APFDIGMWLTVEAPAYAVWIDPEDGSEYWAG"
     gene            4107..4727
                     /locus_tag="ACFMWZ_001616"
     CDS             4107..4727
                     /locus_tag="ACFMWZ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935554.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPD
                     GSPAKAVVLKANQNGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            4730..5665
                     /locus_tag="ACFMWZ_001617"
     CDS             4730..5665
                     /locus_tag="ACFMWZ_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC5935555.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDEKKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDHEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT         1897 a         1183 c         1444 g         1531 t
ORIGIN      
        1 ggcgcgattg gtgcggtcgt gggggagatt gtcggggagg ctttggttaa gaataccgat
       61 ttcagcggta tgactgcttc tgaaattgaa aaagctaaag cgaatattac tgcgtatgca
      121 aaattggtag ccggagcgac tgtaggtgtt acaggaggca atgttgatgt ggcggcaaat
      181 gcttccgaaa cagctgttaa aaataatgca ttagatatta tttgggatat cggcaacctc
      241 gtatgggacg gcggtaaatg gatttacgcc aaatctattg gcgataagca gatggctcga
      301 gaagcggcga ttgattttgg tgtggatgcc gccgcagctg ccgttccctt tgttccggca
      361 ggtgcgacta aaatcagccg aggcggggct tatgttctga aggcgggaga cgaagcagtt
      421 gatacggcta aagccataca ggaaattcag aagcagaccg gaatcaagct tacttatgat
      481 aaggttaata aggtttggac aacaccggcg gggttagatt atgggttaga tgctaagcat
      541 ggtaatagga ttaaacatgt tttagcccat acaattccaa atccaaacaa acctgttcat
      601 tctgttttta atgtgtcccg taaagaagtt ttgcctttgg ttgatgaagc ttggagaatg
      661 aaaggaaatc ctttgccaaa tgattcatcc gtatatcttg tagatatgaa gaaacctatt
      721 ggaacaaaag gagaaacaaa agtgcggatt gttgtgcaaa aaggaacaaa taaaatcatt
      781 tctgcatatc ctcagaaata attaagaaag gaatctctta tggataaaga aattaaaatt
      841 tgcccaagat gtgagcaagg ctacctttat catgcaaagc ctaaatattt ctctggggag
      901 gtcattttat gcgatgaatg ttatgctatg tggcttgggg atatgaaaat tttttacgga
      961 caatatggaa aagattttta tgattatcat gagtttatga aagataaagg catagaagaa
     1021 ataaatatgt gggaaggaga gctttttgat cacccatatt atgaggatga aaaatttaaa
     1081 taattgattt tctgttcccc gaatttggga aatacgatga tattttaaac ccaaatatta
     1141 tttaaagtag caataggccg tctgaatatc cgtttttcag acggcctcaa tgcaactgct
     1201 ggcagccgaa ggcattcacc aacaccaatt gaatgttcag aaaagtaccc gtttcatcgg
     1261 catcaaagtg ggtaaaagca attacagcaa aaacgagctg aacgaaacca aactgcccgt
     1321 acgcgttatc gcccaaacag ccaaaacccg ttccggctgg gataccgtac tcgaaggcac
     1381 cgaattcaaa accacccttt tcggagccga catacaggca ggggtgggtg aaaaagcccg
     1441 agccgatgcg aaaattatcc taaaaggcat cgttaaccgc atccaaaccg aagaaaagct
     1501 ggaatccaac tcgaccgtat ggcaaaagca ggccggaagc ggcagcacgg ttgaaacgct
     1561 gaagctaccg agctttgaag ggccggcact gcctaagctg accgctcccg gcggctatat
     1621 cgccgacatc cccaaaggca acctcaaaac cgaaatcgaa aagctggcca aacagcccga
     1681 atatgcctat ctgaaacagc ttcagacggt caaggacgtg aactggaacc aagtacagct
     1741 cgcttacgac aaatgggact ataaacagga aggcctaacc ggagccggag ccgcaattat
     1801 cgccctggca gtagcggcgg taaccggagg agtaggcgca ggccttatcg gagcggcaca
     1861 gggtaccgca tcggcagccg tggccgatgc tgcctttacc gcattggtga gccaggcttc
     1921 catttccctg atcaacaaca aaggcaatat cggtaacacc ctgaaagagc tgggcagaag
     1981 ccgcacggtg aaaaatctgg tggttgccgc cgctaccgca ggcgtagccg acaaaatcgg
     2041 cgcttcggca ctgaacaata tcagcgataa gcagtgggtc aatgacctaa ccgtcaacct
     2101 ggccaatgcg ggcagtgccg cgctgattaa taccactgtt aacggcggca gcctgaaaga
     2161 caatctggaa gcgaatatcc ttgcggcttt ggtcaatacc gcgcatggag aggcagcaag
     2221 taaaatcaaa cagttggatc atcattacat tacccacaag attgcccatg ccatagcggg
     2281 ctgtgctgcg gcagcggcga ataagggcaa gtgtcaggat ggtgcgatcg gcgctgcggt
     2341 cggtgaaatc cttggcgaaa ccctactgga cggcagagac cctggcagcc tgaatgtgaa
     2401 ggacagggca aaaatcattg ctaaggcgaa gctggcagca ggggcggttg cggcgttgag
     2461 taagggggat gtgagtacgg cggcgaatgc ggcggctgtg gcggtagaga ataattcttt
     2521 aaatgatata caggatcgtt tgttgagtgg aaattatgct ttatgtatga gtgcaggagg
     2581 agcagaaagc ttttgtgagt cttatcgacc actgggcttg ccacactttg taagtgtttc
     2641 aggagaaatg aaattaccta ataaattcgg gaatcgtatg gttaatggaa aattaattat
     2701 taacactaga aatggcaatg tatatttctc tgtaggtaaa atatggagta ctgtaaaatc
     2761 aacaaaatca aatataagtg gggtatctgt cggttgggtt ttaaatgttt cccctaatga
     2821 ttatttaaaa gaagcatcta tgaatgattt cagaaatagt aatcaaaata aagcctatgc
     2881 agaaatgatt tcccagactt tggtaggtga gagtgttggt ggtagtcttt gtctgacaag
     2941 agcctgcttt tcggtaagtt caacaatatc taaatctaaa tctcctttta aagattcaaa
     3001 aattattggg gaaatcggtt tgggaagtgg tgttgctgca ggagtagaaa aaacaatata
     3061 cataggtaac ataaaagata ttgataaatt tattagtgca aacataaaaa aataggagtt
     3121 agtatgaaat atatgattaa ttttctaaaa aaaacatttg aattaatgag ttgggtatta
     3181 gtcatactaa taattgggac attttatgac tattatcaaa taaggcaata tgctgaatta
     3241 gaaaagaaat ctatatcaaa tatcttgcta tatgcccaaa aagaaaaatt tcgcttagag
     3301 agtaaagata aatacatgcg aggaggatat acaaaatatg aatttatttt ttcagaatat
     3361 agtaatacta cttttttaaa cttcataaat gacctgaaaa aagataatta tttaccactt
     3421 gacggctatg gacatggttt tctatgtaga aaaggagagt ctatatcaat caatatatac
     3481 cctgaaatta ataaatttat tttagtatgg ggataccctg aaaatctttg cgctgattct
     3541 aattaagtaa gcaagaatag gttataggga aataaaattc aaatgagaaa attgaataat
     3601 cacgatgttc ataaacggta tcaagatcgt ttagaagagg atgtagagtt cactatcaac
     3661 tatgagcttc ctttgagttg tttgtggtca accatcaaag acttttccag cgattttgag
     3721 gaaaaaactg aagcgttctt tattcttttc aaagagctgc tgcgcagagg tcatctgaaa
     3781 ctgcaacgcg acgggcaaat tatcgggcat acgcccgaag aatgggaaca aatatttagg
     3841 gaagtatggt ctgaatatga aatcgaaccc aatccacttc ccggctatgc cccgtttgat
     3901 attggaatgt ggcttacggt cgaggctcct gcctacgccg tatggataga tcccgaagac
     3961 ggtagcgaat actgggcggg ataaaatacc aatgtttgga ataaatcccg tctgaaaaac
     4021 agctttttca gacaggattt attccaaaca ttggtgatat acagagtttt gtacaagcac
     4081 agaccgctgc cgatcacctg tttgctttgc tgggtgtggt tccgggtatc ggtgaatcga
     4141 tacaggccta taaagtagcg aaagcggcaa aaaatttaca aggcatgaaa aaagccttgg
     4201 acaaggcagc aaccgttgcc actgcacagg gctatgtcag taaaaccaaa atcaaaatcg
     4261 gtcaaactga attaagggtt actgcagcaa ctgacaaaca attgctgaaa gctattggcg
     4321 aaggaaggga cacgacaggt aaaatgaccg agcagttatt tgactcttta gctaaacaaa
     4381 atggcttcag agtgctttcg ggcggcaaat acggcggaaa taacggtttt gatcatgtat
     4441 ggcaggctgc cgatggtagt gttgttttga ttgtagaaag taagcagatt aggaacggta
     4501 cggtacagct gaatccgaat ggtgcgggtg gatatacgca gatgagtcgt gaatggatta
     4561 aacaagttgt aaaaagttta cctgatggta gtcctgctaa ggcagttgtc ttaaaagcaa
     4621 atcagaacgg caaattaaaa acggcaatag caggcgttga tcgtcaaaca ggtaaggccg
     4681 ttattctttc tgtcaaagtt ccttctaaaa ccaatataag gagataacaa tggggcacaa
     4741 tatgatgacc acccaaaaat ggtatgaaca tattactaat gtaatcatag gcaatactgc
     4801 taatttcaat agcggttgcc ccgaatctat agattatgta gatgaaaaaa aaggcgtgcc
     4861 gcttgcagcg atgaaataca ttttaatgta cactgaagct gcggcttccc atgcctatct
     4921 atttgaacat gatcttaaga aattcaagca atatgcttat gttgcaggaa agttgggtat
     4981 tttgcagagt gtagatgatg aagaccccga acccttcttc tttccctgcg acatgctcaa
     5041 cattcaagat ccgatgtttc tgatgctgat gagcgacagc ccgcagctgc gcgagttttt
     5101 ggtgcgcaat atcgacaaca tcgccaacga tacagaagcc ttcgtaaacc gatacgacct
     5161 caaccgtcat atgatttaca atactctgct gatggtggag ggtaagcagc ttgatcggtt
     5221 gaaacaacgt agcgagaaag tcttggcgca tcccacccct agcaaatggc tgcaaaagcg
     5281 gttgtacgat taccgcttct tcctcgcttt cgccgaacag gatgccgagg cgatgaaggc
     5341 cgccttagag ccgcttttcg ataaaaaaac cgcgcgtatg gctgccaaag aaacattgtc
     5401 ctatttcgat ttctacctgc agccgcaaat cgttacctat gccaaaatcg catccatgca
     5461 cggtttcgat ttgggcatag accacgaaat cgcaccgagg gatttgattg tttacgatcc
     5521 gctgccagca gacgaatatc aagacatctt cgattttatg aaacagtatg acttgtctta
     5581 cccgtatgaa tatctgcagg attggataga ttactatacg ttcaaaaccg ataagctggt
     5641 atttggcaac gcgaagcgag agtgagccgt aaaactctga gctcctgttt tatagattac
     5701 aactttaggc cgtcttaaag ctgaaagatt ttcgaaagct ataaattgaa gcccttccac
     5761 agtacataga tctgtgttgt ggcggggctt taccacgctg attgccggag aagaactcaa
     5821 cctgctggca aaacaaggca tgagatcttt gcaataacat gagttgagac ctttgcaaaa
     5881 aagcccttcc ccgacatccg aaacccaaac acaggatttc ggctgttttc gtaccaaata
     5941 cctcctaatt ttacccaaat atccccttaa tcctccccgg atacccgata atcaggcatc
     6001 cgggctgcct tttaggcggc gcgggcgcac ttagcctgtt ggcggccttc aacag
//
LOCUS       DAXHKX010000123         6022 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHKX010000123 DAXHKX010000000
VERSION     DAXHKX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.123"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFMWZ_001618"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFMWZ_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC5935556.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYEAAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFMWZ_001619"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFMWZ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC5935557.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFMWZ_001620"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFMWZ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC5935558.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1347 a         1703 c         1628 g         1344 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcacctt ccgccgaata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgtt tgtaacgcag accgaccaaa
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaagg cggattggac ggcggcttca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcaaacg accaaacgcc cgtgccgctt
      481 ttgaacagcc cgcccaattc ggcagaatgc gtatcgacgc ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagcttc
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaggg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcaca
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcataa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctact gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcgtgcc aacccaaatc gacggcgcgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg taggcgttca
     1501 gacgtcatgg ttgcttaatg ttggctgtcg tccgaaccgg tgatgcccgc ttcggcggcg
     1561 tgttttactg ccatttcgtg ttcgtgcgcg gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa cacggacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gtcgtgccga tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcgaa cgaggtcatg ataatcggac ggaaacgcag gcgggcggct
     1801 tccaaagcag cttcgaccgc gcttttccct tgcgcttgaa ggtctttggc aaattcgata
     1861 atcaaaatcg cgtttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccattcgaag
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cacttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccggcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattgttg cggtcttgca ggttgatgct caggcccgaa
     2701 ccattgccca actccaaaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgt
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga acgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactgttgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggaaaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtat gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgtaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcagaag tggacagata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcgcccg gataagtggc cctcagggtg atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttaat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcgaaaa tggggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ccggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcgacc gttacctcgc ggggttccat accgccttgg gcgttcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacgatg aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gtcggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt aatgccggca gatttgattg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtaccgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgtgcgctt tccagacctg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg atgatgccgc cgacttgggc gcggacatcg gcggtacgca
     5761 gcgattccaa acgccccggc aactcgacgg tcaatgcgac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggagcc ggtgcttccc gaccagcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc at
//
LOCUS       DAXHKX010000124         5879 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHKX010000124 DAXHKX010000000
VERSION     DAXHKX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.124"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..410)
                     /locus_tag="ACFMWZ_001621"
     CDS             complement(<1..410)
                     /locus_tag="ACFMWZ_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFC5935559.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVG"
     gene            complement(446..559)
                     /gene="rrf"
                     /locus_tag="ACFMWZ_001622"
     rRNA            complement(446..559)
                     /gene="rrf"
                     /locus_tag="ACFMWZ_001622"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(656..3547)
                     /locus_tag="ACFMWZ_001623"
     rRNA            complement(656..3547)
                     /locus_tag="ACFMWZ_001623"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3954..4029)
                     /locus_tag="ACFMWZ_001624"
     tRNA            complement(3954..4029)
                     /locus_tag="ACFMWZ_001624"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3994..3996),aa:Ala,seq:tgc)
     gene            complement(4035..4111)
                     /locus_tag="ACFMWZ_001625"
     tRNA            complement(4035..4111)
                     /locus_tag="ACFMWZ_001625"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4075..4077),aa:Ile,seq:gat)
     gene            complement(4212..5752)
                     /locus_tag="ACFMWZ_001626"
     rRNA            complement(4212..5752)
                     /locus_tag="ACFMWZ_001626"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1269 a         1730 c         1270 g         1610 t
ORIGIN      
        1 ccgacggaag cctgatcctt cagcttcgcc agcaccgcag ggccgatgcc ctttaccttg
       61 gtcaaatcgt ccacagactt gaacgcaccg ttttgcgcac ggtattccgc aatggccttc
      121 gccttcgccg ggcctatgcc tggcagcgcc tccaactcct gcagcgaagc cgcattgatg
      181 tttaccgccg caagggagaa ggcgcaggag aacagcatac agaacagcac gaacattttc
      241 ttcatggttt ttcctttaag ggttgcaaac aataaaccgc atcttgcgac gataaaacgg
      301 gtcattctaa aatgaatatc ccaaagtttc aagccgttcc tccgcaaacc cgaccggacg
      361 ccgtacggat gccgtcccgc catccccgac attttttccg ggcaaagcaa aaacccccga
      421 ataatcgggg gttttctgaa tgggtgtttg gcagtgacct actttcgcat ggaagaacca
      481 cactatcatc ggcgctgagt cgtttcacgg tcctgttcgg gatgggaagg cgtgggacca
      541 actcgctatg gccgccaaac ttaaactgtt acaaatcggt aaagccttaa tcaatatatt
      601 cggtaatgac tgaatcagtc agtaagcttt tatctcttga agttcttcaa atgatagagt
      661 caagcctcac gagcaattag tatgggttag cttcacgcgt taccgcgctt ccacacccca
      721 cctatcaacg tcctggtctc gaacgactct ttagtgcggt taaaccgcaa gggaagtctc
      781 atcttcaggc gagtttcgcg cttagatgct ttcagcgctt atctcttccg aacttagcta
      841 cccggctatg caactggcgt tacaaccggt acaccagagg ttcgtccact ccggtcctct
      901 cgtactagga gcagcccccg tcaaacttcc aacgcccact gcagataggg accaaactgt
      961 ctcacgacgt tttaaaccca gctcacgtac cactttaaat ggcgaacagc catacccttg
     1021 ggaccgacta cagccccagg atgtgatgag ccgacatcga ggtgccaaac tccgccgtcg
     1081 atatgaactc ttgggcggaa tcagcctgtt atccccggag taccttttat ccgttgagcg
     1141 atggcccttc catacagaac caccggatca ctatgtcctg ctttcgcacc tgctcgactt
     1201 gtcggtctcg cagttaagct accttttgcc attgcactat cagtccgatt tccgaccgga
     1261 cctaggtaac cttcgaactc ctccgttacg ctttgggagg agaccgcccc agtcaaactg
     1321 cctaccatgc acggtccccg acccggatga cgggtctggg ttagaacctc aaagacacca
     1381 gggtggtatt tcaaggacgg ctccgcagag actggcgtct ctgcttctaa gcctcccacc
     1441 tatcctacac aagtgacttc aaagtccaat gcaaagctac agtaaaggtt cacggggtct
     1501 ttccgtctag cagcgggtag attgcatctt cacaaccact tcaacttcgc tgagtctcag
     1561 gaggagacag tgtggccatc gttacgccat tcgtgcgggt cggaacttac ccgacaagga
     1621 atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct tcgatccgat
     1681 gctctcacat cttcaattaa ccttccagca ccgggcaggc gtcacaccct atacgtccac
     1741 tttcgtgtta gcagagtgct gtgtttttaa taaacagtcg cagccaccta ttctctgcga
     1801 ccctccgggg cttacggagc aagtccttaa ccttagaggg cataccttct cccgaagtta
     1861 cggtatcaat ttgccgagtt ccttctcctg agttctctca agcgccttag aattctcatc
     1921 ctgcccacct gtgtcggttt gcggtacggt tcgattcaaa ctgaagctta gtggcttttc
     1981 ctggaagcgt ggtatcggtt gcttcgtgtc cgtagacact cgtcgtcact tctcggtgtt
     2041 aagaagaccc ggatttgcct aagtcttcca cctaccggct taaacaagct attccaacag
     2101 cttgccaacc taaccttctc cgtccccaca tcgcatttga atcaagtaca ggaatattaa
     2161 cctgtttccc atcgactacg catttctgcc tcgccttagg ggccgactca ccctacgccg
     2221 atgaacgttg cgcaggaaac cttgggcttt cggcgagcgg gcttttcacc cgctttatcg
     2281 ctactcatgt caacattcgc acttctgata cctccagcac actttacaat gcaccttcat
     2341 cggcctacag aacgctcccc taccatgccg gtaaaccggc atccgcagct tcggttatag
     2401 atttgagccc cgttacatct tccgcgcagg acgactcgac cagtgagcta ttacgctttc
     2461 tttaaatgat ggctgcttct aagccaacat cctggctgtc tgggccttcc cacttcgttt
     2521 accacttaat ctatcattgg ggaccttagc tggcggtctg ggttgtttcc ctcttgacaa
     2581 cggacgttag cacccgctgt ctgtctcccg aggaaccact tgatggtatt ctgagtttgc
     2641 catgggttgg taagttgcaa taacccccta gccataacag tgctttaccc ccatcagtgt
     2701 cttgctcgag gcactaccta aatagttttc ggggagaacc agctatctcc gagtttgttt
     2761 agcctttcac ccctatccac agctcatccc cgcattttgc aacatgcgtg ggttcggtcc
     2821 tccagtacct gttacggcac cttcaacctg gccatggata gatcactcgg tttcgggtct
     2881 acacccagca actcatcgcc ctattaagac tcggtttccc tacgcctccc ctattcggtt
     2941 aagctcgcta ctgaatgtaa gtcgttgacc cattatacaa aaggtacgca gtcacacctc
     3001 tagggtgctc ccactgtttg tatgcatcag gtttcaggtt ctgtttcact cccctcccgg
     3061 ggttcttttc gcctttccct cacggtactg gttcactatc ggtcgatgat gagtatttag
     3121 ccttggagga tggtcccccc atattcagac aggatttcac gtgtcccgcc ctacttttcg
     3181 tacgcttagt accactattg agatttcgaa tacgggacta tcacccacta tggtcaagct
     3241 tcccagcttg ttcttctatc tcgacagtta tcacgtacag gctcctccgc gttcgctcgc
     3301 cactacttgc ggaatctcgg ttgatttctt ttcctccggg tacttagatg gttcagttct
     3361 ccgggttcgc ttctctaagt ctatgtattc aacttaggat actgcacaga atgcagtggg
     3421 tttccccatt cggacatcgc gggatcattg ctttattgcc agctcccccg cgcttttcgc
     3481 aggcttacac gtccttcatc gcctatcatc gccaaggcat ccgcctgatg cacttattca
     3541 cttgactcta tcatttcaag aacttctttg actttgccta acattccgtt gactagaaca
     3601 tcagacttga atttcctact ttgataaagc ttactgcttt gttgtgtctt aatcctgcct
     3661 tttgtgtttc aggattaagt cgatacaatc atcacccaaa tactgtgttt gttttctttt
     3721 ctcttgtgag cgatttttta tcctttgcaa agaataaaaa atcaaaacaa acgctttgtc
     3781 tttgtttgtt gatttcggct ttccaatttg ttaaagatcg atgcgttcga tattgctatc
     3841 tactgtgcaa atcaaaacga gctgattatt atatcagcat tttgttcttg gtcaagtgtg
     3901 acgtcgccct gaatggattc tgttccattc ttccgttttg atttgtacag tattggtgga
     3961 ggcaaacggg atcgaaccga tgaccccctg cttgcaaagc aggtgctcta ccaactgagc
     4021 tatgcccccg ttcttggtgg gtctgggagg acttgaacct ccgaccccac gcttatcaag
     4081 cgtgtgctct aaccagctga gctacaaacc cggattctct tcttaagcga atcttgcctt
     4141 cactcaagct tcttccgcat cttttcagtt taccgataag tgtgaatgcc taaagcctct
     4201 tctttctcta gaaaggaggt gatccagccg caggttcccc tacggctacc ttgttacgac
     4261 ttcaccccag tcatgaagca taccgtggta agcgggctcc ttgcggttac cctacctact
     4321 tctggtatcc cccactccca tggtgtgacg ggcggtgtgt acaagacccg ggaacgtatt
     4381 caccgcagta tgctgacctg cgattactag cgattccgac ttcatgcact cgagttgcag
     4441 agtgcaatcc ggactacgat cggttttgtg agattggctc cgcctcgcgg cttggctacc
     4501 ctctgtaccg accattgtat gacgtgtgaa gccctggtca taagggccat gaggacttga
     4561 cgtcatcccc accttcctcc ggcttgtcac cggcagtctc attagagtgc ccaactgaat
     4621 gatggcaact aatgacaagg gttgcgctcg ttgcgggact taacccaaca tctcacgaca
     4681 cgagctgacg acagccatgc agcacctgtg ttacggctcc cgaaggcact cctccgtctc
     4741 cagaggattc cgtacatgtc aagaccaggt aaggttcttc gcgttgcatc gaattaatcc
     4801 acatcatcca ccgcttgtgc gggtccccgt caattccttt gagttttaat cttgcgaccg
     4861 tactccccag gcggtcaatt tcacgcgtta gctacgctac caagcaatca ggttgcccaa
     4921 cagctaattg acatcgttta gggcgtggac taccagggta tctaatcctg tttgctaccc
     4981 acgctttcgg gcatgaacgt cagtgttgtc ccaggaggct gccttcgcca tcggtattcc
     5041 tccacatctc tacgcatttc actgctacac gtggaattct acctccctct gacacactcg
     5101 agtcacccag ttcagaacgc agttcccggg ttgagcccgg ggatttcaca tcctgcttaa
     5161 gtaaccgtct gcgcccgctt tacgcccagt aattccgatt aacgctcgca ccctacgtat
     5221 taccgcggct gctggcacgt agttagccgg tgcttattct tcaggtaccg tcatcagccg
     5281 ctgatattag caacagcctt ttcttccctg acaaaagtcc tttacaaccc gaaggccttc
     5341 ttcagacacg cggcatggct ggatcaggct tgcgcccatt gtccaaaatt ccccactgct
     5401 gcctcccgta ggagtctggg ccgtgtctca gtcccagtgt ggcggatcat cctctcagac
     5461 ccgctactga tcgtcgcctt ggtaggcctt taccccacca accagctaat cagatatcgg
     5521 ccgctcgaat agcgcaaggc ccgaaggtcc cctgctttct ctctcaagac gtatgcggta
     5581 ttagctgatc tttcgatcag ttatccccca ctactcggta cgttccgata tgttactcac
     5641 ccgttcgcca ctcgccaccc gagaagcaag cttctctgtg ctgccgtccg acttgcatgt
     5701 gtaaagcatg ccgccagcgt tcaatctgag ccaggatcaa actcttatgt tcaatctcta
     5761 actttttaac ttctggtctg cttcaaagaa accgacagga caatgttcaa aacattatct
     5821 tgtctgtctt tcaaacagtg tgagactcaa ggcactcaca cttatcggta atctgtttt
//
LOCUS       DAXHKX010000125         5843 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHKX010000125 DAXHKX010000000
VERSION     DAXHKX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.125"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            47..430
                     /locus_tag="ACFMWZ_001627"
     CDS             47..430
                     /locus_tag="ACFMWZ_001627"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC5935560.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            545..1018
                     /locus_tag="ACFMWZ_001628"
     CDS             545..1018
                     /locus_tag="ACFMWZ_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC5935561.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1144..2247
                     /gene="mnmA"
                     /locus_tag="ACFMWZ_001629"
     CDS             1144..2247
                     /gene="mnmA"
                     /locus_tag="ACFMWZ_001629"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC5935562.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYEGDVCLGGGIIQSTDKPVIITR"
     gene            2318..3988
                     /locus_tag="ACFMWZ_001630"
     CDS             2318..3988
                     /locus_tag="ACFMWZ_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC5935563.1"
                     /translation="MKKIWLDSYEKGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4100..5734
                     /locus_tag="ACFMWZ_001631"
     CDS             4100..5734
                     /locus_tag="ACFMWZ_001631"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC5935564.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1519 a         1594 c         1487 g         1243 t
ORIGIN      
        1 ggtttttgtt aatccactat attgagaaca ttttgaaagg ataccgatgg aaccttcttc
       61 ttacgcggca gaaaaaaaag gcaaaggcgg catcaggcgc gtcattaacg cattcggcta
      121 ttcgatagac ggcatcgccg ccgcctaccg ttacgaagcg gcattccgtc aggttttgtg
      181 gctgaacgta ctgctggtgt gcgcggcatt tttttgggtt tccgaaaccg ccgtccgcct
      241 gccgttgatt atcgcgtctt ttgtgtcggt cattgtcgaa ctgttcaaca ccgccgtcga
      301 agctgccgtc gatcatactt cgaccgaaaa acacgagctg gccaaacgcg ccaaagacgc
      361 aggttctgcc gcacaattgt tcgcgatgct gatgttggcg gcggtttggc tgtctgccct
      421 gttcgggtaa aacgcttgca gcaggatttg taatccttta ggattagtat gtggtattta
      481 ttattcactt aatccactat aagattaagc acattccccg tctgatatta taaggagcag
      541 aaagatgaaa aaattattgg cggccgtgat gatggcaggt ttggcaggcg cggtttccgc
      601 cgccggagtc cacgttgagg acggctgggc gcgcaccacc gtcgaaggta tgaaaatagg
      661 cggcgcgttc atgaaaatcc acaacgacga agccaaacaa gactttttgc tcggcggaag
      721 cagccccgtt gccgaccgcg tcgaagtgca tacccacatc aacgacaacg gcgtgatgcg
      781 gatgcgcgaa gtcgaaggcg gcgtgccttt ggaggcgaaa tccgttaccg aactcaagcc
      841 tggcagctat cacgtgatgt ttatgggttt gaaaaaacaa ttaaaagagg gcgacaagat
      901 tcccgttacc ctgaaattta aaaacgccaa agcgcaaacc gtccaactgg aagtcaaaat
      961 cgcgccgatg ccggcaatga accacggtca tcaccacggc gaagcgcatc agcactaatc
     1021 tgctgaaaat gtttgaaatg ccgtctgaaa aagcccgggc tttcagacgg catttttatg
     1081 cccgccttta aaatgtgtta aaatccgcct ttaaaaaccg ccgtttccaa ccatattcta
     1141 cgcatgaata cgacagcaaa caccaccaat atcatcgtcg ggctctccgg cggtgtcgat
     1201 tcttccgtaa ccgccgccct gctcaagcag cagggttatc aagtgcgcgg tgtgttcatg
     1261 cagaactggg aagacgacga caacgacgaa tattgcagca tcaaacagga ttcgttcgat
     1321 gccatcgccg ttgccgatat tatcggcatc gatatcgaca tcgttaattt cgccgcgcaa
     1381 tataaagaca aagtctttgc ttattttctt caggaataca gtgcggggcg cacgccgaat
     1441 cccgatgtgt tgtgcaacgc cgaaatcaaa ttcaaatgct ttttggatta cgccgtaggg
     1501 cagggcgcgg ataccatcgc caccggacac tatgcgcgca aagaagtccg caacggcgtg
     1561 cattacctgc tcaaaggttt ggatcgaaac aaagaccaaa gctatttcct ctaccgcctc
     1621 aagcctttcc aactcgagcg cgcgattttt ccgttgggcg gtttggaaaa acccgaagtc
     1681 cgccgccttg ccgccgagtt caaactgcca accgctgcca aaaaagacag cacgggcatc
     1741 tgcttcatcg gcgaacgtcc gtttcgcgag ttcctgcaga aatacctgcc gaccgacaac
     1801 ggtaaaatgg ttacgcccga agggaaaacc gtcggcgaac acgtcgggct gatgttctac
     1861 acgctcggac agcgcaaagg tttgggcatt ggcggtgcgg gcgaaccgtg gtttgttgcg
     1921 gctaaagatt tgacgaaaaa cgaactgatt gtcgtacaag gacacgacca tccgctgctc
     1981 tatacccgca gccttgtgat gaacgatttg agtttcacgc tgcccgaacg cccgaaagca
     2041 gggcgttata cctgtaaaac gcgttaccgt atggcggacg cgccttgtga gttgcgctat
     2101 ttggatgaag aaaccgccga gctggtgttt gacgaaccgc aatgggcggt aacgcccggt
     2161 caatccgccg tgctgtatga gggcgatgtc tgcttgggcg gcggcattat ccaatcaacc
     2221 gacaaacccg tcatcatcac gcgataaagg taatgccgtc tgaaacggtt ttcagacggc
     2281 attgttccgc tcaattccac tttaaagacc gatacctatg aaaaaaatct ggttagacag
     2341 ctacgagaag ggcgtgaatg ccgaaatcga catcacgcaa tacggttccg tcagcgatgt
     2401 attttgccaa agtgtcaaga aatttgccgg ccaacctgct tttcaaaaca tgggcaagac
     2461 gctgacttat gccgaaaccg gcaaactggc aacagatttt gcttcttatc tgcaaaacgt
     2521 cctgaagctg ccgcgcggcg aacgtgttgc cattatgctg cccaacgtat tgcaataccc
     2581 gattgccctt ttcggcattt tgcaggcagg tttggtagcg gtgaacacca atccgctcta
     2641 taccccgcgc gagctggagc atcagttgaa agacagcggc gcgaccgcca tcatcgtttt
     2701 ggaaaatttc gccaacacgc tggagctggt gctgccgcgc acgcagatca aacacgtcat
     2761 cgtagcctcc gtcggcgaaa tgttcgggct gcttaaaggt tcgctgatca atttcgtcat
     2821 ccggaaaatc aagaaaatgg tgcccgaata ccgtattcgg gaaaccattt cctttcagac
     2881 ggcattgaaa gagggggcga agcacgtttt ccaacctgtc gcattaaacc gcgaagatac
     2941 cgcgctgttg caatacacgg gcggcacgac aggtgttgcc aaaggggcgg tgctgagtca
     3001 cggcaacatc tgcgccaata tgcttcaggc aaaagaatgg attaaaaacc agttgcgcga
     3061 gggcaaagaa accgttatcg ccgccctgcc gctgtatcat atttttgcct taaccgtgaa
     3121 tctgatgatt tttgccaatg ccggctcgaa aattgtcctg attaccaacc cgcgcgatat
     3181 gaaaggcttt atcggcgaac tgaaaaaaca gcgggttaat gtatttatcg gcgtaaacac
     3241 gctgtttaac gcgatggtta accggcccga ttttgccgaa gtcgattttt cagaattgcg
     3301 gctgactttg ggcggcggta tggcgaccca aaaagccgtt gccgaaaaat ggaaaaaaat
     3361 caccggcacg cccatcgtcg aagcctacgg tttgaccgaa gccagccccg gcgtgtgctg
     3421 caacccctta aacatcgaat catacagcgg cagcatcggt ttgcccgtct cgtccaccga
     3481 agtcgaactg cgcgacgcaa acggcaaaga agtccccgtc gggcagccgg gcgaattgtg
     3541 ggtaaaaggc cctcaagtga tgcaaggcta ctggaaccgc cccgaagaaa ccgccaaagc
     3601 catagacgcg cgcggcttct tggaaaccgg cgatattgcc gtcatggacg aaaaaggctg
     3661 gttgaagctg gtcgatcgta agaaagacct cgtcgttgtt tccggattca atgtttatcc
     3721 gaacgaaatc gaggaagtca tcgcgcatca cggcaaagtc atggaagtcg cctgtatcgg
     3781 cgttcccgac gaaaaaaccg gcgaggcact caaagtattc gtcgtcaaaa aagacccgtc
     3841 tttgaccaaa gaagaactta tcgccttttg ccgcaccgaa ttaaccgcat ataaagtacc
     3901 gaaaaatatc gaattccgcg acgagttgcc caagtccaac gtcggcaaaa tcctgcgccg
     3961 cgagttgcgg caaagtaccg ggaaataaag aaaagatgcc gtctgaaaac agccgtccac
     4021 cgttcagacg gcatccgtcc gtttgccaga accgcgcgct tcacgttaaa atcacgcatt
     4081 ccaacatggg tattccatca tgaccaaatt cattttcgta accggcggcg ttgtctcctc
     4141 actgggtaaa ggtatcgccg ccgcttctat tgccgccatc ctcgaatcgc gcggcttgaa
     4201 cgttaccatg ctcaagctcg atccttatat caacgtcgat cccggcacga tgagcccgtt
     4261 ccaacacggc gaagtgttcg taaccgacga cggcgcggaa accgacctcg acttgggaca
     4321 ctacgaacgc tttatcgatt ccacgatgac ccgccgcaac agcttcagca cggggcaggt
     4381 gtacgaaaac gtcatcgcca aagaacgacg gggcgactat ttgggcggca cggttcaagt
     4441 catcccgcac attaccgacg aaatcaaacg ccgcatccac gaaggcgcgg caggttacga
     4501 tgtggcgatt gtcgaaatcg gcggcacggt cggcgacatc gaatcgctgc cgtttttgga
     4561 agccatccgc cagatgcgaa gccagttggg acgcaacaac accctgttcg cccacttgag
     4621 ctacgtcccc tacatcgccg ctgcaggcga aatcaaaacc aagccgaccc agcacaccgt
     4681 taaagaaatg ttgagcatcg gtttgcaacc cgacatcctg atttgccgta tggacaggac
     4741 aatgcctgcg gacgaacgcc gcaaaatcgc cttgttctgc aacgtggaag aacgcgcgat
     4801 tgtcggcagc tacgatgtgg acagcatcta cgaatgcccc gaaatgctgc acgaccaagg
     4861 catcgacaac atcattaccg agcagttgca gcttaatgtg cagcaggcgg atttgaccgc
     4921 gtggaaaaaa atcgtccacg ccatccaaaa cccgaaacac accgtcaaaa tcgccatggt
     4981 cggcaaatac gtcgatttga ccgagtccta caaatcgctg atcgaagcct tgaaacacgc
     5041 aggcattcac accgaaaccg acgtacaaat tacctttgtt gacagcgaaa gcatcgagaa
     5101 aaacaacggc gacgtttcta tgcttaaaga tatggacgcc atcctcgttc ccggcggttt
     5161 cggttcgcgc ggcgtggaag gcaaaatcgc cgctgtgcgc tatgcccgcg aaaacaacgt
     5221 gccatacttg ggcatctgcc tcggtatgca gattgcgctg atcgaatacg cccgcgacgt
     5281 ggcaggtttg aaaggtgcga attccaccga gttcgacctc aaatgcgccg cccccgtcgt
     5341 tgccctgatt gacgaatggc aaactgccga cggcagcgtc gaaacccgcg acgaatccgc
     5401 cgatttgggc ggcaccatgc gtttgggcgc gcaagaagtc gaattgaaag caggcagcct
     5461 cgccgccaaa atctacggca gcgaacacat ccgcgaacgc caccgccacc gctacgaagt
     5521 caacaacaac tacgttccta cgctggaaca ggcaggtttg gtcatcggcg gcgtatccgc
     5581 cggacgcgaa cgcttggtcg aaaccatcga actgccgaac catccttggt tcttcgcctg
     5641 ccagtttcat cccgaattca cttccaaccc gcgcaaaggg catcccttgt tcaccgcgtt
     5701 tgtcaaagct gcgttgaaca ataaaaaagc ctgataaagc gttacttgat gatcaaaatg
     5761 ccgtctgaaa gcctgacaaa gactttcaga cggcattttc gcaagtcggg gattataaaa
     5821 gccatcaatt taatttttat ggt
//
LOCUS       DAXHKX010000126         5791 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHKX010000126 DAXHKX010000000
VERSION     DAXHKX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.126"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(130..891)
                     /locus_tag="ACFMWZ_001632"
     CDS             complement(130..891)
                     /locus_tag="ACFMWZ_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935565.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            991..1242
                     /locus_tag="ACFMWZ_001633"
     CDS             991..1242
                     /locus_tag="ACFMWZ_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935566.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1294..2100
                     /locus_tag="ACFMWZ_001634"
     CDS             1294..2100
                     /locus_tag="ACFMWZ_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC5935567.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2407..3930
                     /locus_tag="ACFMWZ_001635"
     CDS             2407..3930
                     /locus_tag="ACFMWZ_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC5935568.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGAAACEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            4028..5440
                     /gene="trkA"
                     /locus_tag="ACFMWZ_001636"
     CDS             4028..5440
                     /gene="trkA"
                     /locus_tag="ACFMWZ_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC5935569.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAETGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1458 a         1731 c         1405 g         1197 t
ORIGIN      
        1 ttcagcttcg tcgccttgtc ctgatttttg ttaatccact atattaatat tttgcgcctg
       61 ttctatgatg ctttcaagtc gggtgagaat gcaaatgccg tctgaaacgg ctttcagacg
      121 gcatggcaat cagcgtttgt attttaactc gtacttgatg tcgttgagga ttttgcggac
      181 atcgtgttcc aacacgtctt cgactaccgc acccgcctgc tcgtgcagca tctgctggag
      241 ctgataggta aaaaccgcca tctgcttttg caccgccgta cggatgatgc cgttgacggt
      301 atcggtcaga tgcgggcgca ggcgtttgat cagccgttcg gtcagctcct gttcggacag
      361 gcagaacact tcgcgccggt tgacggcttt cggattcagg atattgattt ggacgggcat
      421 caacgcctct tccgcaccgc cttcgccgtt ttccgcaacc ccgtctgccg ccgtacccga
      481 ttctgccgca tcggcgtttt ccccgccttc aatctgtccg gtttcaaatt cggcactgtc
      541 ttttttggca tcgaacagga ttctccgccg cgattcgatg tgtttttccg aaaccgacat
      601 ttgcagggaa gcctgcgcgt tgagccagtt ttcctgaagg acaatcatcg ggtcggtttc
      661 gacttcctcg ccgcaatcgg caacggcggc attgtgttcc tcctgccatt ttttcagata
      721 cgccttcaac acacgggctc ggctctcatc gtccagtttc ggcacaggcg cgtccgttcc
      781 ggtttcagag gggcgggaca gcggcgcgta agtcggcact gccttcatac ggcgcgtctg
      841 acgcaggttt tccaaacgtt tttcccaatt cggctcttta ttcgcatcca ttttcggctt
      901 ccggttctta atctttgcaa gcagacaaac ccgcgcccaa agcgcggttt gatataatgg
      961 cgcattttaa cagattcgcg aggatacatc atgggcagca tcgaacagcg tttggaatat
     1021 ctggaagagg cgaacgacgt gctgcgtatg cagaaccacg tcctgtccac cgcattcaaa
     1081 gccttaatcc gcgcccttcc cgccgaaacc gccgaaatcg cggtcgagtc gattcagctt
     1141 gcttttgagg acgccttggc agaattgagc tatgaggaca gcccccatac cgatttgttc
     1201 cacgacgtta cttatgcgtt tttccgtgaa aaagaacgtt aattttatgt taaactgatt
     1261 ttttaggctt tttgattacc gaaaggaatt ttgatgaata tgaaaaaatg gattgccgcc
     1321 gcccttgcct gttccgcgct tgcgctgtct gcctgcggcg gtcagggtaa agatgccgcc
     1381 gcgcccgccg caaatcccga caaagtgtac cgcgtggctt ccaacgccga gtttgccccc
     1441 tttgaatctt tagactcgaa aggcaatgtc gaaggtttcg atgtggattt gatgaacgcg
     1501 atggcgaagg cgggcaattt taaaatcgaa ttcaaacacc agccgtggga cagccttttc
     1561 cccgccttga acaacggcga tgcggacgtt gtgatgtcgg gcgtaaccat taccgacgac
     1621 cgcaaacagt ctatggactt cagcgacccg tattttgaaa tcacccaagt cgtcctcgtt
     1681 ccgaaaggca aaaaaatatc ttcttccgac gatttgaaaa acatgaacaa agtcggcgtg
     1741 gtaaccggct acacgggcga tttctccgtt tccaaactct tgggcaacga caatccgaaa
     1801 atcgcgcgct ttgaaaacgt tcccctgatt atcaaagaac tggaaaacgg cggcttggat
     1861 tccgtggtca gcgacagcgc agtcatcgcc aattatgtga aaaacaatcc gaccaaaggg
     1921 atggacttcg ttaccctgcc cgacttcacc accgaacact acggcatcgc ggtacgcaaa
     1981 ggcgacgaag caaccgtcaa aatgctgaac gatgcgttgg aaaaagtacg cgaaagcggc
     2041 gaatacgaca agatttacgc caaatatttt gcaaaagaag acggacaggc cgcaaaataa
     2101 gcccgcccgt ccgaacacaa tgccgtctga agccctttca gacggcattg ttcatcaatc
     2161 ggcctacaat gaactgcctg ctgatttctc cctaccgcaa agcaacaggc aaagattaca
     2221 aatatcaaaa tccgagtaaa acagtatttt attaaaacaa attgataatc aagagattag
     2281 aattatgtat tgtctttacc gtacaaacgc tggcactatt tcaacctgat aaaaaacagc
     2341 cttcaaaaag gttgcttaaa acagcagcag acacttaccg ccacaacctt gaaaaggaac
     2401 acaatcatga ccgtcatcaa acaggaagac tttatccaaa gcatttgcga tgccttccaa
     2461 ttcatcagct actaccatcc caaagactac atcgacgcgc tctataaagc gtggcagaaa
     2521 gaagaaaacc ccgccgccaa agacgcgatg acgcagattt tggtcaacag ccgcatgtgt
     2581 gccgaaaaca accgccccat ctgccaagat accggtatcg cgaccgtctt cctcaaagtc
     2641 ggtatggatg tgcagtggga tgcggacatg agcgtcgaag aaatggttaa cgaaggcgtg
     2701 cgccgcgcct acacttggga aggcaatacg ctgcgcgctt ccgttctcgc cgaccccgcc
     2761 ggcaaacgcc aaaataccaa agacaacacg cccgccgtca tccatatgag catcgtgccg
     2821 ggcgacaaag tcgaagtaac ctgcgcggca aaaggcggcg gttctgaaaa caaatccaaa
     2881 ctcgccatgc tcaacccttc cgacaacatc gtcgattggg tattgaaaac cattccgacc
     2941 atgggcgcgg gctggtgtcc tcccggcatc ttgggcatcg gcatcggcgg tacgcccgaa
     3001 aaagccgtgt tgatggcgaa agaatccctg atgagccaca tcgacatcca agaattgcag
     3061 gaaaaagccg cgtccggcgc ggaattgtcc accaccgaag ccctgcgcct cgaactcttt
     3121 gaaaaagtca acgcgctagg catcggcgcg caaggcttgg gcggtctgac caccgtgttg
     3181 gacgtgaaaa tcctcgatta cccgacccac gccgcctcca aaccgattgc catgattccg
     3241 aactgcgccg ccacccgcca cgtcgaattt gaattggacg gctcaggccc tgtcgaactc
     3301 acgccgccgc gcgtcgaaga ctggcccgat ttgacttaca gccccgacaa cggcaaacgc
     3361 gtcgatgtcg acaagctgac caaagaagaa gtggcaagct ggaaaaccgg cgacgtattg
     3421 ctgttgaacg gcaaaatcct caccggccgc gatgccgcac acaaacgcct cgtcgatatg
     3481 ctcaacaaag gcgaagaatt gcccgtcgat ttcaccaacc gcctgattta ctacgttggt
     3541 cccgtcgatc cggtcggcga tgaagtcgtc ggcccggcag gccccaccac cgccacccgc
     3601 atggacaaat tcacccgcca aatgctcgaa caaaccggcc tcttgggcat gatcggcaaa
     3661 tccgaacgcg gcgcggcggc gtgcgaagcc atcgccgaca acaaagccgt gtacctgatg
     3721 gcggtcggcg gcgcggcata cctcgtggca aaagccatca aatcttccaa agtcttggcg
     3781 ttccccgaat tgggtatgga agccatttac gaatttgaag tcaaagatat gcccgtaacc
     3841 gtcgccgtag acagcaaagg cgaatccatc cacgccaccg ccccgcccca atggcaggcg
     3901 aaaatcggca tcatccccgt caaatcttga ggcgtcatgc cgtctgaaca caaaatctgc
     3961 tttcagacgg catttccgcc cccggttgcg gtacaatcca ccatttcatc actcggcgac
     4021 ccacaccgtg aaaatcctca ttttaggcaa cggacaggta ggttctaccg tcgcacaaaa
     4081 ccttgccgcc atacccaaca acgacgtaac cgttatcgac atcgacgaaa aagcattgca
     4141 ggaaacaggc agccgcctcg atgtccaaac cgttttcggc aacggcgcat cccccttcac
     4201 attagaacgc gccggcgcgg aagatgccga cttgctgctc gcgctctccc gcagcgacga
     4261 aaccaacatc gtcgcctgca aagttgccgc cgacctgttc aacatccccg gccgcatcgc
     4321 gcgcgtccgt tccagcgaat acctcgaata cctcagcccc aagctcgaaa acaacgaaaa
     4381 cggcagcctt tccatattcg gcataaccga aaccatcagc cccgaacagc tcgttaccga
     4441 acagcttgcc ggcctgatag actgcccggg cgcattgcag gttttacgtt ttgcagacga
     4501 ccgcgtgcgg atggtcatca tacaggcgcg gcgcggcggg ttgcttgtcg gacgcagcat
     4561 tgccgacatc gcccaagatt tgcccgacgg ggccgactgc caaatctgcg ccgtttaccg
     4621 caacaaccgc ctcatcgtcc ccgcgccgca aaccgtcatc atcgaaggcg acgaaatcct
     4681 atttgccgcc gccgccgaaa acatcggcgc ggtcataccc gaattgcgcc ccaaagaaac
     4741 cagcacccgc cgcatcatga ttgccggcgg cggcaacatc ggctaccgtc tcgccaagca
     4801 gctcgaacac gcatacaacg tcaaaatcat cgaatgccgg ccgcgccgtg ccgaatggat
     4861 agccgaaaac ctcgacaaca ccctcgtcct gcaaggttcg gcaaccgacg aaaccctgct
     4921 cgacaacgaa tacatcgacg aaatcgacgt attctgcgcc ctgaccaacg acgacgaaag
     4981 caacattatg tccgcccttt tggcgaaaaa cctcggagcg aagcgcgtta tcggcatcgt
     5041 caaccgctca agctacgtcg atttgctcga aggcaacaaa atcgacatcg tcgtctcccc
     5101 ccacctcatc accatcggct cgatactcgc ccacatccgg cgcggcgaca tcgttgccgt
     5161 ccaccccatc cggcgcggca cggcggaagc catcgaagtc gtcgcacacg gcgacaaaaa
     5221 aacttccgcc atcatcggca ggcgcatcag cggcatcaaa tggcccgaag gctgccacat
     5281 tgccgccgtc gtccgcgccg aaaccggcga aaccattatg ggacaccata ccgaaaccgt
     5341 catccaagac ggcgatcaca tcatcttttt cgtctcgcgc cggcgcatcc tgaacgaact
     5401 ggaaaaactc atccaagtca aaatgggctt tttcggataa accgccccat tccggacaca
     5461 ttgccgccaa gcggtatgga agcggaataa tggtaggtgg gcttcagacg gcatccgccc
     5521 tccccgtcat tcccgcgtaa gcgggcatcc agaccttggg atagcggcaa tattcaaagg
     5581 ttatctgaaa atttagaggt tctagattcc cgttttcacg ggaatgacga agagttgcgg
     5641 gaatctagac cattggaaag cggcaatatt caaaggttag ctgaagcttt agaggttcta
     5701 gattcccgtt tccacgggaa tgacgaaagg tggcgggaat ccagaacgtc gggcaacggc
     5761 aatattcaaa agccgtctga aaatttaaaa g
//
LOCUS       DAXHKX010000127         5709 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHKX010000127 DAXHKX010000000
VERSION     DAXHKX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.127"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..251)
                     /locus_tag="ACFMWZ_001637"
     CDS             complement(<1..251)
                     /locus_tag="ACFMWZ_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC5935570.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIGNF"
     gene            complement(557..1816)
                     /gene="rho"
                     /locus_tag="ACFMWZ_001638"
     CDS             complement(557..1816)
                     /gene="rho"
                     /locus_tag="ACFMWZ_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC5935571.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2036..4420)
                     /gene="ppsA"
                     /locus_tag="ACFMWZ_001639"
     CDS             complement(2036..4420)
                     /gene="ppsA"
                     /locus_tag="ACFMWZ_001639"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC5935572.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPIDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4831..5652
                     /locus_tag="ACFMWZ_001640"
     CDS             4831..5652
                     /locus_tag="ACFMWZ_001640"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC5935573.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIREIIKQADAFHINFFETFLGLLEKE
                     LNTEAVAAEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESTDLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1224 a         1343 c         1631 g         1511 t
ORIGIN      
        1 caaaagttcc caattccatt gatatggttt tgtcgttcag caaaataact ttcatctgct
       61 tctacttcgc cgtcaaacat ttccaaatgc ggactgtttt gataaataag taatcgtaaa
      121 cgatgaaaat aataggctgc ggtactttta ttaacgccta ctaactctgc tgctgttctt
      181 gcagttacac ctgcgacaaa cagttcaatg agtttatttt gtttatactg gcttagacga
      241 ctttttctca taggggcaac tctaacttaa tttgaatttc cctagttatc taggacagcc
      301 ccaaatttat acaaaaatga gcgcggttcg gcgcaacctt gaatcaagtt cccgcatcgg
      361 ttttcattgc cggtacggat gcgttcaagc cggctttgca aaggccgcgc tttcggcaag
      421 cggacacgga cactgccgac ggttgcgccg ttaacggggg gagggaggag ctgcgccgac
      481 cgtgtgaatg aaagtgccgt ctgaaacccg attttcaggc ttcagacggc atttcgcatt
      541 aatgcgggcg gcgcgtttat ttgccgcgca tcagttcaaa gaaatcgtcg ttgtttttag
      601 aggctttgat tttcccgttt aagaactcgg tagcttcgat ttcatccatc gggtgcagga
      661 acttgcgtaa gagccacata cgttgcaact ggtcgttcgg cacaagcagc tcttcgcggc
      721 gcgtgccgga tttgttgatg ttgatggcgg ggaagaggcg tttttccgcc atacggcgat
      781 caaggtgcaa ttccatattg ccggtgcctt tgaattcttc gtaaatcaca tcgtccatac
      841 ggctgccggt ttcaaccaat gcggtggcga taatggtcag cgaaccgcct tcttccacgt
      901 tgcgcgccgc accgaagaaa cgtttggggc ggtgcagcgc gttggcatcg acaccgccgg
      961 ttaggatttt gcccgaggta ggcacgacgg tattgtaggc gcgggcaagg cgggtaatcg
     1021 aatccagcag gatgaccacg tcttttttgt gttctaccat acgcttggct ttttcaagca
     1081 ccatttcggc aacttggacg tggcgttgcg ccggttcgtc aaaggtggag gaaaccactt
     1141 cgccgcggac ggaacggctc atttcggtta cttcttcggg acgctcgtca atcaagagga
     1201 cgatgagttc gacttcggga tagtttgagg taacggcgtg ggcaatgttt tgcagcatca
     1261 cggttttacc gcttttgggc ggggcaacca agagggcgcg ctgacctttg ccgatagggg
     1321 aaatcaggtc gatggcacgt ccggtcaggt tttcttcgga ctttaagtcg cgttccagct
     1381 tcaactgttc ggtcggaaac agcggggtca ggttttcaaa caggatttta tggcggcata
     1441 cttccgggtg gtcgccgttg atggtatcaa gcctgaccag ggcaaaatag cgttcgttgt
     1501 cttttgggac gcgcacgctt ccttcgatgg tgtcgcccgt atgcaggttg aagcggcgga
     1561 tttgggtggg cgagacatag atgtcgtcgg ggccggcaag ataggacgtg tccgcgctgc
     1621 ggaggaagcc gaagccgtcg ggcaggattt caagcgtgcc ggagcaggtg aaaccctcgc
     1681 cttttttcat catctggcgg acgatggcaa atacgaggtc ttgtttgcgg aatcggttgg
     1741 cgttttcgat gccgtgttct tccgccaatt ctaagagttt ggaaatgtgc agggtttgta
     1801 attcggagac gtgcataata atgatgtatt ttgaagagga aaaagacagg cagatgccgt
     1861 ctgaaagaag aagctgaccg ttgccggttg ctcggggaag ggggaattgt aggcagtcgg
     1921 cgcgtgggtg tcaaatatta tcgcggacgg ggcatcggca ggaaatgccg tctgagcgga
     1981 gctgcttgga aaaaaatacc cccgcgcttt tcaggctcgg gggtatgggc attgattatt
     2041 tgttcaattc attcgccaaa tacagccaag tttcgatgac ggtatccggg ttcagggaaa
     2101 cgctttcaat gccttcctca accagccatt tggcgaagtc cggatggtcg gacgggcctt
     2161 gaccgcagat gccgacatat ttgttctgct tgcggcaggc ggagatggca aggtgcagca
     2221 tcactttgac ggcagggttg cgttcgtcaa acgattcgga taccaagccg ctgtcgcggt
     2281 caagaccgag ggtcagttgg gtcatgtcgt tcgagccgat ggagaagccg tcgaagtatt
     2341 gcaggaattg ttccgccaat accgcgttgc tcggcagctc gcacatcata atcaggcgca
     2401 ggccgttttt gccgcgttcc aagccgtttt ctttcagggc tttgacaacg gcttcggctt
     2461 cgcccaaggt gcggacgaac ggaatcatga tttcaacgtt ggtcaacccc atttcatcgc
     2521 ggacgcgttt caaggctttg cattccaagg cgaaacagtc tttgaagttg tcggcgacat
     2581 aacgcgccgc gccccggaag cccaacatcg ggttttcttc atgcggttcg tatacgttgc
     2641 cgccgaccag gttggcgtat tcgttggatt tgaagtcgga catacggacg atggttttac
     2701 gcggataaac cgatgcggcc aaagttgcca cgccttctgc gattttatcg acatagaagt
     2761 caataggaga ggcgtagccg gcgatgcggc gggtaatttc cgctttcaat tcgtcgtctt
     2821 gtttgtcaaa ttccaacaag gctttggggt ggataccgat ttggcggttg atgataaatt
     2881 ccatacgcgc caagccgatg ccttcgctgg gcaggttggc gaagctgaat gcgagttcgg
     2941 gattgccgac gttcatcatg actttaaccg gtgctttggg catattgtct aaggcgacat
     3001 cggtaatctg tacgtccaac agaccggcat agataaagcc ggtatcgcct tcggcacagg
     3061 atacggtaac ttcttgaccg tttttcagca attcggttgc attgccgcag ccgacaacgg
     3121 caggaatgcc caattcacgc gcgatgatgg cggcgtggca ggtacggccg ccgcggttgg
     3181 tcacgatggc agaagcacgt ttcatcacgg gttcccaatc cggatcggtc atgtcggtaa
     3241 cgagtacgtc gccggcttcg acggaatcca tctcggaagc atctttaatc aggcgcacct
     3301 tgccctgacc gactttctga ccgatggcgc ggccttcgca taatacggtt ttgtcgccgt
     3361 tgatggcgaa gcggcgcagg ttgcggttgc cctcttcttg ggattttacg gtttcgggac
     3421 gggcttgcag gatgtagagt ttgccgtcca agccgtcgcg tccccattcg atatccatcg
     3481 ggcggccgta gtgtttttcg atggtcagtg cgtaatgcgc caactcagta atttcttcgt
     3541 cggtaatgga gaagcggttg cggtcttcct cggggacatc gacgttggtt acggatttac
     3601 cggcttctgc tttgtcggta aaaatcattt tgatgtgttt tgaacccatg gttttacgca
     3661 ggatggcggg cttgcccgct ttgagcgtgg gtttgaacac ataaaattcg tccgggttga
     3721 ccgcaccttg tacgacgttt tcgcccagac cgtaagagga ggtaacaaag acgacttgat
     3781 cgtagccgga ttcggtgtcg agggtgaaca tcacacctga tgcgccgctg tcggaacgca
     3841 ccatgcgttg aacgccggcg gaaagggcga cgatgtcgtg ttcgaagcct ttgtggacac
     3901 ggtaagaaat ggcacggtcg ttgtacaggg aggcgaatac atggtgcatc gcttctttaa
     3961 cgttttccaa tccgttgatg ttcaagaagg tttcctgttg gccggcaaat gatgcgtcgg
     4021 gcaggtcttc ggcggttgcg gaagagcgta cggcaacgga aatgtccgca ccgccggcat
     4081 cggcaaccat tttgttccat gccgcttcga tttcggcatc gagctgttcg gggaagggcg
     4141 tgtccaaaat ccattggcgg atttctttgc cgacgcgtgc cagttcggca acgtcttcga
     4201 catctaattt tgccagtgcg gcggaaatgc gttcgctcag accgttgtgt gcgaggaatg
     4261 cgcggtaggc atcggctgtg gtggcaaagc cgccggggac gcgaacgcct ttttcggtca
     4321 gctgactgat catttcgccc agcgaggcgt ttttaccgcc cacgcgttca acatctgtca
     4381 tacgcaggtt ttcaaaccag attacgtagt tgtcggccat ttgtgtgtcc aatccaaaat
     4441 atgttaaaaa agaaacaaat ccgcgtgctt attttaagcg attcgttcct ctgctgtcat
     4501 gtgttttatc cgttttaaaa tcatgatgcc gtctgaaaaa ttgcggtttc ggcgtgtgta
     4561 gcggtttgaa acttgcagcc ggtatacttc tttttttggg tattttcttt gtaaaacagg
     4621 tggtttgaat aggttaatgt tttttctgtt tgattttttt gtttattttt taaaattttc
     4681 tgccaaaaaa tactttatat aaataatttt atttcaaaat tatattgtgt ctgtttgggt
     4741 gtaatccgag gtaggcgtgc tgcgggtgct ttccttgtgt ctgccgctgc tgttatgatg
     4801 atatttttat tctgtattta aggatggaag atgagcagtc cgcgccatgt gttttacatt
     4861 tccgaccgta ccggtctgac tgccgagaat atcggcgagg ctttgctgaa ccagtttggc
     4921 aatctgtcgt tcaaacgcca tacgcatccg tttgtcgata cgccggaaaa ggcgcgcgcg
     4981 gtggtggaga aggtcaatcg gagccggcag gaaaacggtc agcgtccgat tgcgtttgtc
     5041 agtgtggttg atgacgaaat ccgtgaaatc atcaaacagg cagacgcttt ccacatcaat
     5101 ttttttgaaa ctttcctcgg gcttttggaa aaggaactca acaccgaggc ggttgccgcc
     5161 gaacagggac accacagtat cggtaatacg aagcgttacg atgcgcgtat ggaggcggtc
     5221 aatttttctt tgaaccacga tgacggggtc agcgataaga accttcagga agcggatgta
     5281 atcttgatgg gtgtatcgcg ttcgggcaaa acgccgacct gcctttacct cgccctgcaa
     5341 tacggcatcc gtgcggcaaa ctatccgctg attcccgacg atttggaatc gaccgatctg
     5401 ccgcgtatgg tcaagccgta taaagacaag ctgttcgggt tgactatcca gccggaacgt
     5461 ttgcaggcaa tccgtcagga acgccgcccg aattcggctt atgcgcgcat cgacacctgc
     5521 cgcagcgagg tggcggacgc gcagagtatg ttcagacggc atggcattcc gtttgcgaat
     5581 acgacggata aatcggtgga agagcttgcc gtgcacatcc ttcaggcgtg caagctcaaa
     5641 cgcaggtttt gatgggcttt gattcggttt gaaggtggaa atgccgtctg aaatcaggtt
     5701 tcagacggc
//
LOCUS       DAXHKX010000128         5700 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHKX010000128 DAXHKX010000000
VERSION     DAXHKX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.128"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="ACFMWZ_001641"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="ACFMWZ_001641"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC5935574.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAEAAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            1136..3001
                     /gene="msbA"
                     /locus_tag="ACFMWZ_001642"
     CDS             1136..3001
                     /gene="msbA"
                     /locus_tag="ACFMWZ_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC5935575.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLFALQTANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3101..4666
                     /gene="guaA"
                     /locus_tag="ACFMWZ_001643"
     CDS             3101..4666
                     /gene="guaA"
                     /locus_tag="ACFMWZ_001643"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC5935576.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVIALRAVITSDFMTAHWAELPYSLLGKVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4728..5474
                     /gene="fabG"
                     /locus_tag="ACFMWZ_001644"
     CDS             4728..5474
                     /gene="fabG"
                     /locus_tag="ACFMWZ_001644"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC5935577.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1379 a         1824 c         1383 g         1114 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaac
       61 aaaggaatac tatgtctttt gccttctttt ttcccggaca aggttcccaa agcctcggta
      121 tgatgaacgg ctttgccgaa cacgccatcg tcaaaaacac ctttgccgaa gcctccgcca
      181 tattggggca ggacttgtgg gcgatgatta acggcagcga tgccgaaatc atcggacaaa
      241 ccgtcaatac ccagcccatc atgctcgccg ccggcgttgc cgtttaccgc gcctatttag
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg aattttgccg acgcggtcaa actcgtgcgc ctgcgcgccg
      421 aactgatgca gtccgccgta ccgcaaggcg tgggcgcaat ggcggcgatt ctcggcttgg
      481 aagatgagca ggtaaaacaa atttgcgccg aagccgccca aggcgaagtg gtcgaagccg
      541 tcaacttcaa ctcacccgga caaatcgtga ttgcaggcaa tgccgccgcc gtcggacgcg
      601 ccatggctgc cgccaaagaa gccggtgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctgatg aaacccgccg ccgacaaact cgccgaagcc ctcaaaaccg
      721 tcgaaatcaa gcagccgcaa atccgcgtca tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacgg
      841 aaaccgtcaa cgcccttgtt tcagacggca ttgccgaatc cgccgaatgc ggcccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaggccgc cgcgtgcagc gcgctgaccg
      961 atgccggaca ggttgccgcc tttatcgaag cacactgact tcgttccgca aaaagcagcc
     1021 tgccctcttc aggctgcttt tcacgtccga acggcggcag ccccgtattt acgctataat
     1081 ccatcccgac caaaccaccg acagcggctg ccgttgcagt tcccgcccta ccgatatgat
     1141 agaaaaactg actttcggac tgtttaaaaa agaagacgcg cgcagcttta tgcgcctgat
     1201 ggcatacgtc cgcccctaca aaatccgcat cattgccgcc ctgattgcca ttttcggcgt
     1261 tgccgccacc gaaagctacc ttgccgcctt catcgccccc ctgattaacc acggcttttc
     1321 cgcacccgcc gcgccgcccg agctgtctgc cgccgccggc atcatttcca ccctgcaaaa
     1381 ctggcgcgaa cagtttacct atatggtttg ggggacggaa aacaaaatct ggaccgtccc
     1441 cctcttcctc atcatcctcg tcgtcatccg tggcatctgc cgctttacca gcacctatct
     1501 gatgacttgg gtctccgtga tgaccatcag caaaatccgc aaagatatgt ttgccaaaat
     1561 gctgaccctt tcctcccgct accatcagga aacgccgtcc ggcaccgtac tgatgaatat
     1621 gctcaacctg accgaacagt cggtcagcaa cgccagcgac atcttcaccg tcctcacgcg
     1681 cgacacgatg atcgttaccg gcctgaccat cgtcctgctt tacctcaact ggcagctcag
     1741 cctcatcgtc gtcctgatgt tccccctgct ctccctgctc tcgcgctact accgcgaccg
     1801 tctgaaacac gtcatttccg actcgcaaaa aagcataggc acgatgaaca acgtgattgc
     1861 cgaaacccat cagggacacc gcgtcgtcaa gctgttcaac gggcaggcgc aggcggcaaa
     1921 ccggttcgac gcggtcaacc gcaccatcgt ccgcctcagc aaaaaaatca cgcaggcaac
     1981 ggcggcacat tccccgttca gcgaactgat cgcctcgatc gccctcgccg tcgtcatctt
     2041 catcgccctg tggcaaagcc aaaacggcta caccaccatc ggcgaattta tggcattcat
     2101 cgtcgcgatg ctgcaaatgt acgcccccat caaaagcctt gccaacatca gcatccctat
     2161 gcagacgatg ttcctcgccg ccgacggtgt atgtgcattt ctcgacaccc cgcccgaaca
     2221 ggacaagggc acgctcgcac cgcagcgtgt cgaagggcgc atcagcttcc gcaacgtcga
     2281 tgtcgaatac cgttcagacg gcatcaaagc cctcgacaac ttcaacctcg acatcagaca
     2341 aggcgaacgc gtcgccctgg tcggacgttc cggcagcggc aaatccaccg tcgtcaacct
     2401 gctgccccgc tttgtcgaac cgtctgccgg caacatctgc atagacggta tcgacatcgc
     2461 cgacatcaaa ctcgactgcc tgcgcgccca attcgccctc gtctcccaag acgtattcct
     2521 gtttgacgac accctgtttg aaaacgtccg atacagccgt cccgacgcgg gcgaagccga
     2581 agtcctgttc gccctccaaa ccgccaacct gcaaagcctg attgacagct ccccgctcgg
     2641 actgcaccag cccatcggat cgaacggcag caacttatcc ggcggacagc ggcaacgcgt
     2701 cgccattgcc cgcgccattt tgaaagacgc gccgatatta ttattggacg aagccaccag
     2761 cgcattagac aacgaatccg aacgcctcgt ccaacaggcg ctcgaacgcc tgatggaaaa
     2821 ccgcaccggc atcatcgtcg cccaccgcct gaccaccatc gaaggggccg accgcatcat
     2881 cgtgatggac gacggcaaaa tcatcgaaca aggcacacac gaacaactga tgtcccaaaa
     2941 cggttactac acgatgttac gcaatatctc aaacaaagat gccgccgtcc ggacggcata
     3001 aacaaaatgc cgtccgaaat ggtacaatcg ccccgaccct ttcagacggc atcatatccg
     3061 ccgacccatc cgattatctt caatcactgt aaaacccatt atgacccaag acaaaatcct
     3121 catccttgac ttcggttcgc aagttaccca gctcatcgcc cgccgcgtgc gcgaagccca
     3181 cgtttactgc gagctgcatt ctttcgatat gcctttggac gaaatcaaag ccttcaaccc
     3241 caaaggcatc atcctctccg gcggccccaa ttccgtttac gaatccgact atcaagccga
     3301 taccggtatt tttgatttgg gcattccggt tttgggcatc tgttacggca tgcagtttat
     3361 ggcgcaccac ttgggcggcg aagtgcagcc cggcaaccag cgcgaattcg gttatgcgca
     3421 agttaaaacc atagacagcg agctgacacg cggcattcaa gatggtgagc caaacacact
     3481 cgacgtatgg atgagccacg gcgacaaagt gtccaaactg cccgacggtt tcgccgtcat
     3541 cggcaacacc ccgtcctgcc cgattgccat gatggaaaac gccgaaaaac aattctacgg
     3601 catccagttc caccccgaag ttacccacac caaacaaggc cgcgccctgt tgaaccgctt
     3661 tgtcttggat atttgcggcg cacaaccggg ctggacgatg ccgaactaca tcgaagaagc
     3721 cgttgccaaa atccgcgaac aggtcggcag cgacgaagtg attttaggtc tgtccggcgg
     3781 cgtggactct tccgtagccg ccgcgctgat tcaccgcgcc atcggcgacc aactgacctg
     3841 cgtgttcgtc gatcacggtt tgttgcgcct gaacgaaggc aaaatggtga tggatatgtt
     3901 cgcccgcaac ttgggtgtga aagtgataca cgtcgatgcc gaagggcagt ttatggcgaa
     3961 actcgccggc gtaaccgacc ccgagaaaaa acgcaaaatc atcggtgcgg aatttatcga
     4021 agtatttgat gccgaagaaa aaaaacttac caacgccaaa tggttggcac aaggcacgat
     4081 ttaccctgac gtaatcgaat ccgcaggtgc aaaaaccaaa aaagcccacg ccatcaaatc
     4141 gcaccacaac gtcggcggcc tgcctgaaaa catgaagctc aaattgcttg agcctttgcg
     4201 cgatttgttc aaagacgaag tacgcgaatt gggtgtggct ttgggcctgc cgcgcgaaat
     4261 ggtgtaccgt catccgttcc cgggtccggg tttgggcgtg cgtattttgg gcgaagtgaa
     4321 aaaagaatat gccgacctgc ttcgtcaggc agacgatatt ttcattcaag aattgcgcaa
     4381 tactaccgat gaaaacggta catcttggta cgacctgacc agccaggcat tcgccgtgtt
     4441 cctgcccgtc aaatctgtcg gcgtaatggg cgacggccgc acatacgatt acgtcattgc
     4501 cttgcgtgcc gtgattacca gcgactttat gaccgcgcat tgggcggaac tgccgtattc
     4561 cttgttgggc aaagtgtcca accgcatcat caacgaagtc aaaggcatca accgcgtggt
     4621 ttatgatgtg agcggcaaac cgcctgccac catcgagtgg gaataaacag caaacatggc
     4681 tgcaccgtcc ggcgcagtct ttcgattatc ggaaaaaagg aaaaaatatg agcacacaag
     4741 atttaagcgg caaaatcgct ttggtaaccg gcgcatcgcg cggtatcggt gcagcaattg
     4801 ccgacacact ggcggcagcc ggtgccaaag tcatcggtac ggcgaccagt gagagcggtg
     4861 cggcggcgat tagcgagcgg ttggcgcaat ggggcggcga aggccgcgta ttaaattccg
     4921 ccgaacccga aaccgtcgaa aacctgattg ccgacatcga aaaaacgttc ggcaaactcg
     4981 atattctggt caacaacgcc ggcatcaccc gcgacaacct cctgatgcgc atgaaagaag
     5041 aagagtggga cgacatcatg caggtcaacc tcaaatccgt gttccgcgct tctaaagccg
     5101 tcttgcgcgg catgatgaag cagcgcgccg gccgtatcat caacatcaca tccgtcgtcg
     5161 gcgtgatggg caatgccggt caaaccaact atgccgcggc aaaagcaggc ttaatcggtt
     5221 tctccaaatc catggcgcgc gaagtcggca gccggggcat taccgtcaac tgcgtcgccc
     5281 ccggctttat cgataccgat atgacccgcg ccctgccgga agaaacccgc caagccttta
     5341 ccgcccaaac cgccttgggc agattcggcg acgcgcaaga cattgccgat gcggttttgt
     5401 tcctcgcttc cgatcaggcg aaatacatta ccggccagac gctgcacgtc aacggcggca
     5461 tgctgatgcc ctgacggaca gccgtgccga caatgccgtc tgaagccctt tcagacggca
     5521 tttgcattcc caaacaaaat gaacacacac cacaccctgc cctgcccctg cggttcaggc
     5581 acaagctgag acctttgcaa aactcctttc cctcccgaca gccgaaaccc aaacacaggt
     5641 tttcgtctat tttcgcccca aatatctcct aattttaccc aaataccccc ttaatcctgc
//
LOCUS       DAXHKX010000129         5692 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHKX010000129 DAXHKX010000000
VERSION     DAXHKX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.129"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            97..813
                     /locus_tag="ACFMWZ_001645"
     CDS             97..813
                     /locus_tag="ACFMWZ_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC5935578.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="ACFMWZ_001646"
     CDS             962..1864
                     /locus_tag="ACFMWZ_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC5935579.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="ACFMWZ_001647"
     CDS             1907..2680
                     /locus_tag="ACFMWZ_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC5935580.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="ACFMWZ_001648"
     CDS             2792..4480
                     /locus_tag="ACFMWZ_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC5935581.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="ACFMWZ_001649"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="ACFMWZ_001649"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC5935582.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMLADAVAAWSVR"
BASE COUNT         1333 a         1547 c         1564 g         1248 t
ORIGIN      
        1 atttgtgcaa tgctttaatt cctctgacac gatttgaaac acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgccagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgatac ggcgcgaaga agaggcctcc ggcaggggcg accgcgtcgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc acggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggaacgct tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gagagcagta
      961 gatggacagc ctgatgacat tgctttcggt attgataccg atgtttgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggataaggtg ctatcggtct tggtgtatgc
     1081 cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggtgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcatccgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaaacgcg ggtatgtatt gtctgatgct
     1381 gctggtgctc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcaggcgg
     1501 gctgctgttt gccgcatcgg cagacggtgt gtcgtgggtg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtgatgacc gaggcttacg gcgcggtatg
     1621 gggcagtatc gcgcttttga acgatttggc acgagagctg ttcgcgctgg catttattcc
     1681 gctgctgatg aagcgttttc ccgatgcggc agtgggggtc ggcggcgcga ccagtatgga
     1741 tttcacattg cccgtgattc ggggtgcggg cggcttggaa gccgtaccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgctcc gtttctgatg gtggtgtttt ccgctttggg
     1861 ctgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgagttgga
     2041 ggatgctggc agcctgaaac gccaccacgg cggcgcatcc tcgggcggaa acttgccgga
     2101 ggggctgcct gccagccgcc aaattcaatg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa catattcccg acgagtcatc gctgtttgtc agtatcggca cgacgatgga
     2221 agccgtggca tcggagctgg tgagacggcg cggcagcctg cggattatta ccaacaatat
     2281 ccacgtcgcc tccatcgttt cggcgcggac ggattacacg gtcatcatca cttccggagt
     2341 cgtccgccct ttggacggcg gtattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaatgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccttca tcgaagaaga cctcaaaagc ggcaaacacg aaaccatcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttca cgcttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcgccgacgc ggctacacgc ccgaagggct
     3721 gcgcctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtaaccc tgaccaactt tgaaaccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aagaaatggg cgatcgcgaa gtacctgttt cacaaaccat
     3961 ttacatcgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgccgccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggttggcg gctgtttcat
     4681 accttgagca gcggattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccaccca attactgacg ctggcggaac agcagggcgc gaaagtcttt
     4921 aacagcggac agtcgatgcg tgacttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca ttatcatcaa accgctggac ggtatgggcg gtatgggtat tttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcggct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg acgcggcgtg gcgcaggaat tggacggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgcttgc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatc
     5461 atgaaacaaa aaggtttcga tgtggcggca atgcttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgtcgt ctgaaaggct tttgcttcac aaccgtttgg tttgttcggg
     5581 caggcagggt tttccctcag ccggcagaga tacgcttaat gccgtacgga cacaccgccg
     5641 ccccgttttc agacggcata tatagtggat taacaaaaat caggacaagg cg
//
LOCUS       DAXHKX010000130         5669 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHKX010000130 DAXHKX010000000
VERSION     DAXHKX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.130"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            121..1296
                     /locus_tag="ACFMWZ_001650"
     CDS             121..1296
                     /locus_tag="ACFMWZ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC5935583.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFSGTRYAVALSNGTLALDVALKAIGIGVGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            1344..3254
                     /gene="pglD"
                     /locus_tag="ACFMWZ_001651"
     CDS             1344..3254
                     /gene="pglD"
                     /locus_tag="ACFMWZ_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC5935584.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPTNWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            3310..4602
                     /locus_tag="ACFMWZ_001652"
     CDS             3310..4602
                     /locus_tag="ACFMWZ_001652"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC5935585.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAVPLLESGEMKRLADQVIRPFYHNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4656..5492)
                     /locus_tag="ACFMWZ_001653"
     CDS             complement(4656..5492)
                     /locus_tag="ACFMWZ_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC5935586.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIQAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKSLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
BASE COUNT         1327 a         1758 c         1448 g         1136 t
ORIGIN      
        1 cacccccgta cagaccgatt ttgacaacac ctgcggcgcg cgcccgattc ttcggaacac
       61 gccccccttc agacggcata gggtcggaaa tgccgtctga aaaccgacgg acaaaccatc
      121 atgctgaaca ctttcctttc cccgtggccc tgcttcaccc aagaagaagc cgatgccgtt
      181 tccaaagtcc tgctgtccaa caaagtcaat tactggacgg gcagcgaatg ccgcgaattt
      241 gaaaaagaat ttgccgcctt ttctggcacg cgatacgccg tcgccctttc caacggcacg
      301 ctggcactcg atgtcgcgct caaagcaatc ggcataggcg taggcgacga tgtgattgtt
      361 acttcgcgca ctttcttggc ttccgcttcc tgcattgtga acgcgggcgc aaatcccgtg
      421 tttgccgacg tggatttgaa cagccaaaac atcagcgcgg aaaccgtcaa agctgtgctg
      481 accccgaata ccaaagccgt catcgtcgtc cacctcgccg gtatgcccgc cgaaatggac
      541 ggcatcatgg ctttggcaaa agaacataat ctttgggtaa tcgaagactg cgcccaagcg
      601 cacggcgcaa aatacaaagg caaatccgtc ggctctatcg gacacgtcgg cgcgtggtcg
      661 ttctgccaag acaaaatcat gaccaccggc ggcgaaggcg gtatggttac gaccaacgac
      721 aaaaccctgt gggaaaaaat gtggtcgtac aaagaccacg gcaaaagcta cgatgccgtg
      781 taccaccgcg aacacgcgcc cggtttccgc tggctgcacg aaagcttcgg cacaaactgg
      841 cgtatgatgg aaatgcaggc ggcaatcggg cgcattcagc tcaaacgcct gcccgaatgg
      901 actgcctgcc gtcaagcgca cgccgccaag ctggcggaaa gcctaggcaa attcaaaagt
      961 atccgcttgg ttgaagtcgc cgactacatc ggacacgcgc aatataagtt ctacgccttc
     1021 gtcaaacccg aacacctcaa agacggctgg acgcgcgacc gcatcgtcaa cgaactgaac
     1081 gcgcgcaaag tcccctgcta tcaaggtagc tgctccgaag tgtatttgga gaaatccttc
     1141 gacaacacgc cttggcgtcc gaaagagcgg ctgaaaaatg ctgtcgagtt gggcgacacc
     1201 agcctgatgt tcttagtaca tccgacgctg accgacggcg aaatcgcgtt ctgcaaagaa
     1261 cacatcgaag ccgtattgac cgaagccaca cgataaccct tcagacggca tatgccgcct
     1321 gaaagcacat accgccccac gatatgactc tggaaaccct gcttgcccta ccgcgcaaca
     1381 tcaagaaaat ctgtttcctc atacacgatt ttctgatgat tttcattgcc ttttggttta
     1441 cccaaagcct gaaggccgac tactcgaacg aatggttcga ccctaccaac tggcagtctt
     1501 tcctattgac cgcgccgttg accatcggcc tgttcgtcag aatggggctc taccgtgccg
     1561 ttacacgctt catcagcttc cgcatcctct ccaccgcgct ggcaggcagc ctcgcctccg
     1621 ccgtattgtt cttcctcaac acattgattt ttgaagaaag gctgcgcctc gccctgccga
     1681 ttgtctattt cttactgctg tttgtttccg tgaccggctc gcgtatggtt ttgcgcggac
     1741 tgttgtccga acaccccaaa aaacagatga tccccgtcat catttacggc gcggggcagg
     1801 cgggcagaca gcttctcgag gccgtcaaac aaatgcgcga atattccgcc gccgcctttg
     1861 tggacgacga ccccaaactg tggcacaccg tcatctacga ccttgccgtt taccagcccg
     1921 atgccatcgc cttcctcatc gaacgctacg gcgtggaaaa aatcctactc gccatccccg
     1981 gcgcgaccca ggaacaacgc cgccgaatca tcaacaaact ggaagcctat ccgtgcgaag
     2041 tgttgaccat tcccggaatg aaagacctga tggacgggaa aatcagcatc ggcacgctca
     2101 aaaaaatctc tgtgtccgac ctgctcgggc gtgattccgt cgcgcccgac aaccgcctga
     2161 tgagtgccga catcgaaggc aaaaccgtca tggtaaccgg cgcgggcggc tccatcggtt
     2221 cggaactctg ccgccagatt atccgccgcc gccccgaaaa gctgctgctg ttcgagttat
     2281 ccgaattcgc cctgtacgcc gtcgaaaaag aattgcgcga aacctgcatc caaaaacgcc
     2341 tcgacaccga aatcctgccc tttctcggtt cggtgcaaaa ccgcacgctg ctcgaacacg
     2401 tcatgaccgc cttttccgtt gcgaccgtct atcacgccgc cgcctacaaa cacgtcccca
     2461 tggtcgagtt caacaccgtc gaaggcatac gcaacaacat cttcggcaca ctcgagtgcg
     2521 cgcttgccgc cacgacatcg ggcgtggaaa ctttcgtcct catctccacc gacaaagccg
     2581 tccgccccac caacaccatg ggtgccagca aacgcatggc ggaactctgc cttcaggcac
     2641 tcgccgccga acccggacaa aaaacccgct tcagcatggt acgtttcggc aatgttttag
     2701 gttcgtccgg ctccgttgtc ccgctgtttg aaaaacagat tgcagaaggc ggcccgctta
     2761 ccctgaccca ccccgaaatc acacgttatt tcatgaccat acccgaagcc gcccaactcg
     2821 tcatacaggc aggcgcgatg ggtacgggcg gcgacgtatt cgtcctcgac atgggtgaat
     2881 ccgtcaaaat catcgacctt gcccgccaaa tgattaccct aagcggcctc aaacccaaca
     2941 cacccgaaca acccgacggc gacatcgaaa tcctcattac cggactgcgt cccggagaaa
     3001 aactctacga agagctgctc atcggcgaca acgtccgcaa aaccggccat ccgcgcatca
     3061 tgaccgccaa cgagaccatg ctgccgtggc acgagctctc cgccctgctc gaccgcatcc
     3121 gtgcggcctg cgaccgttac gaccagcagg caatccgcac cctgctcatc aacgccccga
     3181 ccggctttgc cccgagcgac ggcatctgcg acctgctttg ggtacgagaa acacacagaa
     3241 aaaatgccgt ctgaaccttc agacggcata acgtacaaac caacctacct tacacacacg
     3301 gagtttgaca tgcagttttc agcattcggc gaaaaattca cgcaacacag cggcatcctc
     3361 caactgatgg acgacctcgg cgacgcgctc aaaagcgaca agcccgtcaa tatgctcggc
     3421 ggcggcaatc cggcgcgaat cgccgaagtc aatgccgtgt tcgccgacgt attttccaaa
     3481 ctggcagcag aacatgccgt cgaaaacatc ggcaactact ccaatcctca aggagatgcc
     3541 gcgctgattg acgcgctgac cgccttcctc aaccgcgaat acggctggaa cctgaccgcc
     3601 gacaatatcg cgctgaccaa cggttcgcaa aacgcgtttt tctatctttt caacctcttc
     3661 ggcggcaaat tcaagctttc agacggcaca tccgcagaaa aagccatttt gttgccgctc
     3721 gcgcccgaat acatcggcta tgccgacgta catatcgaag ggcagcactt cgtttccgtc
     3781 aagcccaaaa tcgaaaacgt cgaacacgaa ggcgaagcag gcttcttcaa ataccgcgtg
     3841 gactttgacg cactggaaaa cctgcccgaa ctcaaagcgg gcaaaatcgg cgcgatttgc
     3901 tgttcgcgcc cgaccaaccc gaccggcaat gtgttgaccg acggcgaaat ggcgcgtttg
     3961 gacgctttgg cgcgtgaaca cggcattccg ctgattatcg acaacgccta cggaatgccg
     4021 ttccccaaca ttatttacag cgatgtaacg ctgaattggc acgaaaacat catcctctgc
     4081 ttcagccttt ccaaagtcgg cctgccgggc gttcgcaccg gcatcatcgt cgccgcgccc
     4141 gaagtcgtca aagccgtcag cagcctgaac gcgattgtga accttgcccc cacgcgcttc
     4201 ggcgcggcga ttgccgtccc gctgctggaa agcggcgaga tgaaacggct tgccgaccaa
     4261 gtcatccggc cgttttacca caatcaggca caaaccgccg tctcgctgct caagcgcgag
     4321 ctgggcgcgt acccgatgaa aatccacaaa cccgaaggcg cgattttcct gtggctctgg
     4381 tttgaaaacc tgcccgtttc ttcgcaaacc ctgtatgaaa tgctcaaagc cgaaggtacg
     4441 ctgattattc cgggcgagca tttcttcgtt ggcatcgaca cgcaggatta cccgcacgcg
     4501 ggcgagtgca tccgcatgag catcgcgcag gacgctcaaa cgctggaaaa aggcattgcc
     4561 gccatcggta aaaccgtccg aaaactgtac gacaacgttt aaaacgcaaa aaaaatgccg
     4621 tctgaaaggt tttcagacgg catttttatc tgcattcaat gtcgggaaaa atgcgcccac
     4681 accggcctgc agttttccgg cagttcggga cacgcgccga gaatgccgcc gctgaagtcg
     4741 tttaaacggt ggaaatcgct gcccgcgctg gcgagcatac cgaagcgttc tgccaaaagc
     4801 gcgtagttga ggcggtcgtt tttgcagcag ttgccgctgt ggacttcgat gcccgcgccg
     4861 ccgaggcttt taaattcttc aaacagattg cgcttggcgg tagcggacaa atcgtagcgc
     4921 atggggtggg cgatgactgc catgccgccc gcgccgttga cggcggagac gcagtcttcc
     4981 agcgtcgccc attcgtggcg gacggcgcag gatttaccgt cgcccaagta tttggtgaac
     5041 gcctgctgct tgtttttgac atgtcccgct tggatgagga actcggcgac gtgggtgcgg
     5101 ctgaccattt ctttgtttgc cgccagtgcc agcgcgccgt cgtatgcacc gccgatgcct
     5161 ttcttttcga gcttggcggc gatggcttca agacgtttca gacggccttg ccgcacttgc
     5221 gccaacaagt tttgcaggtt ttcgtcctgc tcgtcgaaat ccaaaccgac aacgtgtatg
     5281 gtacgcccgc gccacgttac ggaaatttcc acaccattaa tcaggcgcaa accgagcctg
     5341 tcggcttcgg cacgcgcttc ggcgatgccg ccggtgtgat cgtggtcggt caacgccagc
     5401 agcgtgcagc cgttttgatg cgcgaggcgg acgacttcgg cgggggaaag cataccgtcg
     5461 gaaacggtgg aatggcagtg caggtctatc atggggtgtt atatgtgtgg tgaatgaagg
     5521 tcggggggca atattgtcgg gcggtgtggc ggcggcggtc attttaaaaa acaggtgtat
     5581 ggcaaatgca aaggaaaagc cgctatgccg tcattcccaa cccccgaatg tgcggataga
     5641 gccagtgggg atgggtacat tatttgttt
//
LOCUS       DAXHKX010000131         5666 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHKX010000131 DAXHKX010000000
VERSION     DAXHKX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.131"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..208
                     /locus_tag="ACFMWZ_001654"
     CDS             <1..208
                     /locus_tag="ACFMWZ_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935587.1"
                     /translation="FSELYMFQQRTAHQAPHCVLPERDYPPIRFYRHKQTGFNRKGMG
                     IKSISDTSRAMPSENQSPLSDGIV"
     gene            285..1232
                     /locus_tag="ACFMWZ_001655"
     CDS             285..1232
                     /locus_tag="ACFMWZ_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC5935588.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVALHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1383..2507)
                     /gene="rluD"
                     /locus_tag="ACFMWZ_001656"
     CDS             complement(1383..2507)
                     /gene="rluD"
                     /locus_tag="ACFMWZ_001656"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC5935589.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2506..3309
                     /locus_tag="ACFMWZ_001657"
     CDS             2506..3309
                     /locus_tag="ACFMWZ_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC5935590.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSLHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATVRMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3367..5586)
                     /locus_tag="ACFMWZ_001658"
     CDS             complement(3367..5586)
                     /locus_tag="ACFMWZ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC5935591.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1354 a         1805 c         1374 g         1133 t
ORIGIN      
        1 tttctccgag ctttatatgt ttcaacagag gacggcacat caagcaccgc actgcgtgtt
       61 gcccgaacga gactaccctc cgattagatt ctatcgccat aaacagacgg gtttcaaccg
      121 aaaaggaatg ggaataaagt ccatttccga cacctctcgg gcgatgccgt ctgaaaacca
      181 atctccactt tcagacggca ttgtttaggc acggtcattt tgcaaattaa ggttaaggcg
      241 tgtatgcttg ccttccctta aaaacaaaca cgcaacgcat tattatgaat atcctcagta
      301 aaatcagcag ctttatcgga aaaacatttt ccctctgggc cgcgctcttt gccgccgccg
      361 cttttttcgc gcccgacacc ttcaaatggg cggggcctta tattccttgg ctgttgggca
      421 ttattatgtt cggtatgggt ttgacgctca aaccttccga cttcgatatt ttgttcaaac
      481 atcccaaagc cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct
      541 ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg
      601 gctgctgccc gggcggtacg gcttccaatg tgatggccta tctggcgcgc ggcaatgtgg
      661 ctttgtcggt tgccgttacg tctgtttcca ccctgatttc cccattgctg actcccgcca
      721 tcttcctgat gcttgccggc gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca
      781 tcgtcaaaat ggttttgctc cccattgttt tgggtttgat tgtccataag gttttgggca
      841 gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga
      901 ttatcggcgc ggttgtcggg gcaagcaaag gcaagattat ggaaagcggc ctgctgattt
      961 ttgccgttgt cgcactccac aacggcatcg gctacctgct cggcttcttt gccgccaaat
     1021 ggaccggcct gccttatgat gcacaaaaaa cgctggccat cgaagtcggt atgcaaaact
     1081 cggggctggc cgccgcgctt gccgccgcac actttgccgc tgcgccggtc gttgccgttc
     1141 cgggcgcatt gttcagcgtg tggcacaata tctccggctc gctgctggca acttattggg
     1201 cggccaaagc cggtaaacat aaaaaaccct aaacgccttt catcataatg ccgtctgaac
     1261 ggggcaaacg aaaccggcgg tttctgcccg accccttcag acgcctttgt ttgaaatcgg
     1321 cggggacgat tatccggctg ctccgattcg gctacctgcg ttcgccccgc ctttcaagcc
     1381 gcctactccc ttacataaac cacttcgaca tcgtagtcgt cttcgttcca atcgtcatcg
     1441 tcgtccgcgc cgaatttgtc ctgccattcc tcttcattgc tcaaagacga atccaaaccg
     1501 gcttcaagac ggaggacaga caacaaatga tatatgtcgt ccggaatcgg tgcctcaaac
     1561 gaaacggttt cgccgctttc cggatgggtg aaactcaagc ggtaggcgtg caacgcctga
     1621 cgcgcgccca aacttttaac ggcttctttc accgtgtcgc cgcacggatg gcgcgggttg
     1681 ccgtaaaccg ggtcggcggc aagcggatgg ttggcctcgc gcatatggac gcggatttgg
     1741 tgcgtcctgc ccgtttcgag cgagcattcg atatagctgt gggtaagata gcgttccaac
     1801 actttgacgt gggtaacggc tggtttgccg ccgaatttga cgactgccat tttcaggcgg
     1861 ttgtgcggat cgcgtccgat ttgggtttcg attttaccgt cgaaggggac gatgccgttg
     1921 gcgacggcgc ggtagatgcg tttgaccgtg cgctcttgaa gctgcctcac gagggaattt
     1981 tgcgccggca gggttttggc aaccaccatc agcccgctgg tttccttgtc caaacggtgt
     2041 acgatgcccg cgcgcggtac ttggctcaat tcgggacagt gcgccaacag gccgttgagc
     2101 agcgtccccg tccagttgcc ggccgccgga tgcaccacca gtccggccgg tttgttgacg
     2161 acgatgacgg tatcgtcttc gtaaacgata tccaaagcca taggctctgg aacaaacgcc
     2221 agattttcct cactcggacg gacggttaca caaatttgct cgccgcctat cattttgtct
     2281 ttgggttgcg aaggtttatc gtttacaata accgcgcctt ctttaatcca tgatgtcagg
     2341 cggctgcgcg agtagtcggg cagaagtttc gccaataccg catccaaccg cccgccggca
     2401 agctcgagcg gaacggtcag accgatacgg ttttcagttt cggaagctga cgcaaagtct
     2461 gaatcgtcgc tataatcgga ttcattatca aaggaagtat tctgcatgaa aaaaattctt
     2521 ttaacggttt cattaggttt ggcactgagt gcctgtgcca ctcaaggtac ggtcgataaa
     2581 gatgcccaga ttacccaaga ttggagtgtg gagaagctct atgccgaagc ccaggacgaa
     2641 ttgaacagca gcaattatac gcgggctgtc aagttatacg aaatcttgga atcgcgcttc
     2701 cccaccagcc tccatgcccg gcagtcgcaa ctggataccg catacgccta ttataaagac
     2761 gatgaaaaag acaaggctct ggcggcaatc gaacgcttcc gccgcctcca tccgcagcat
     2821 ccgaatatgg attacgcgct gtatctgcgc ggcttggtgc tgttcaacga agaccagtcc
     2881 ttcttgaaca aactggcctc gcaagactgg tccgaccgcg acccgaaagc caaccgcgaa
     2941 gcgtatcagg cctttgcgga actcgtccaa cgcttcccga acagcaaata cgccgccgat
     3001 gcaaccgtac gcatggtcaa actggtcgat gcactgggcg gcaatgaaat gtcggtggcg
     3061 cgctactaca tgaaacgcgg tgcatacatc gccgccgcca accgcgccca aaaaatcatc
     3121 ggcagctacc agaatacgcg ttatgtcgaa gaatcgctcg ccatattgga acttgcctac
     3181 caaaaactcg gcaaaccaca gcttgccgcc gatacgcgcc gcgtgttgga aaccaacttc
     3241 ccgaaaagcc cgtttttgac gcacgcttgg cagcccgacg atatgccctg gtggcgttac
     3301 tggcattaag cccatcttcg taatgccgtc tgaagccgtt tcagacggca ttttatgttt
     3361 caaacctcat tcaaacggtt ttttctgcca atagaattta taccctctca aacgttgagc
     3421 cttcacgccg ccgcgcccca agccgaattg cagcgcgcct gacaaatcgg tacgcagcag
     3481 tttgatgccg tgtgcgcgga cacggttctg caccgcttcg gtcggatgtt tgtaggcgtt
     3541 ggcataaccg cttgaagcaa cagcatattc gggcgagacg gcattgagga acacgcccga
     3601 cgaggacgta ttgctgccgt gatgtcccaa caccaaaacc tggctgtaca ggttgcctcc
     3661 atacttgccg accaggcttt cctcgccctt cgtatccaaa tcgcccgtta ccagcagtgc
     3721 cgcaccgccc gccacaacac gcaaaacaca acttttccca ttatcatcga tgtttttgcg
     3781 ttcagacggc atcaaaaact caaaatccac gccgtcccat tgccaacgct gttccgcaca
     3841 atgccgcgcc ccctcataaa attccggctg cccggcataa atcccgccgt tcggtatctt
     3901 gccgactgcc tgaaaaccgc cgtcgtggtc gctgtcgtga tgcgacaaaa ccaaataatc
     3961 caaatacctg attcccgacg cattcaagct cggcacaatc cccatctgcg ccgccgctgc
     4021 cgttccggtg tcaaacaaaa gatgatgatt tgccgtctga accgacaccg acaaaccctg
     4081 ccccgcatcc caaaccgtaa ccgcaacctc attttccggc acgccgggtg aacggtaaaa
     4141 cacaaaccct gccaacagca acaccgccca cggacgcaaa cccaagccgc gcggcagcag
     4201 caacagcaaa gcggcacaca ccgccaacac caacagcggc aaaggtgcgg aggcaacggc
     4261 aaactcgggc gacacatcgg caagccacac caaaaaccgc aaagtatatt ccgccaaaaa
     4321 tgcccccaac tgttgcaaag gcgcaaacgg cacgaccgaa cccagcaacg ccagcggcgt
     4381 caatacccaa gaaaaccacg gaattgccac cgcattgacc aaagggctga ttaaaggcag
     4441 cgaagcaaac agataaccga gcaaaaccag cgacaacacc gaagccgccc actgcccgcg
     4501 caaagcagtt tgccgttttc cctcgtgcaa acgccccgag cacgcccata tcagggccgc
     4561 caccaaaccg aaagacagcc aagtccccac acccaagacc gccgacgggt cgaacagcag
     4621 caccgccgcc aacgcctgcc accacgtcgc ccacgccgac aatcttcccc tgcgccaagc
     4681 ccacgcaaac gccgccaaca tcaaaacgct gcgctgcgtc ggcacggaaa aaccggcaag
     4741 cagcgcgtaa aacagcgcgc ctgcacaccc cgccgccaaa acccacgcgc gcggccgggc
     4801 aggaaggcgc ggggaacagg caagcagccg cttcgccagc cacgcaaaca ttaccgccac
     4861 catcgtaacg tgcaaacccg aaatactgac caaatgcgtc agccccaacg gccggaacgc
     4921 ctgccacaat tcggggcgca atgccgactg ttcgcccacg ctcaacgcgc gcatcagccc
     4981 gatgccgtct gaaagcccgc cgtccgcatc cgcctgctgc caattacggc tgatgcggct
     5041 gcgccaaacc gcaatccccc aaccgcttcc gccatgcagc aaaaccctgt ccgcaccgac
     5101 cgtccccgcg ccgcctattc cgttggataa tgcccacgcc tcacggttca aaccgcgcag
     5161 gttcaactcc ccgacgacag ggtgcacacg tgccgttatc cgccatctgc tcccgaccgc
     5221 ccattcgcgc cgtttgtagt ccgacagcag caaatcaaac gtccgaccac cgctgtccac
     5281 agcctttgcc gcaaactgca cacgccgccc gtccgacctc ggcatatccg ccacttccac
     5341 cgtcaaaggc acgcctgaaa cggcttctgc ccaccattgg gaagacaatg ccgcctccgt
     5401 cctgaatacg ccgtaagccg cgcccgccaa cacgcacagc atcagaccgg caaacgcaaa
     5461 acgccttgca aacacagcaa aaaccgcaaa agccgccagc caaaacagcc aatgcggcac
     5521 gaccggcagc gcaaacgaag ccgccgcgcc gacaacccaa cacggcaaaa accaacgacg
     5581 gaacacgtct ccccacccca gatatttcaa aaataaacgg ataataaagc aaatccgaaa
     5641 aaccgtcata acaagatttg aaaatg
//
LOCUS       DAXHKX010000132         5586 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHKX010000132 DAXHKX010000000
VERSION     DAXHKX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.132"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..152)
                     /locus_tag="ACFMWZ_001659"
     CDS             complement(<1..152)
                     /locus_tag="ACFMWZ_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC5935592.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRIR
                     YVRDHRG"
     gene            complement(376..2588)
                     /locus_tag="ACFMWZ_001660"
                     /pseudo
     CDS             complement(376..2588)
                     /locus_tag="ACFMWZ_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258624.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
     gene            complement(3274..3927)
                     /locus_tag="ACFMWZ_001661"
     CDS             complement(3274..3927)
                     /locus_tag="ACFMWZ_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC5935593.1"
                     /translation="MKTTHCKLKKKVQKELLRFFVLEVTARSAADILGIQPDSAILFY
                     RTIRMVISHYPALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLHVSGFIHHRINHSKEFADRQ
                     NHINGIENFWNQAKRALRKYNEIDRKSFPLFLKECKFRFNFGTPSRQLKILRDWCGI"
     gene            4264..4698
                     /locus_tag="ACFMWZ_001662"
     CDS             4264..4698
                     /locus_tag="ACFMWZ_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935594.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQPYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4739..>5586
                     /locus_tag="ACFMWZ_001663"
     CDS             4739..>5586
                     /locus_tag="ACFMWZ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC5935595.1"
                     /translation="MIGSGDTKQCKKFSADEFAKTIQSNFQSGIKYDGPTLATAGVSN
                     IIDEYLKDAYGGNGLKEAQRNLVAKEIADRIEAELHRQRANYQAQEAAKYSPAHGGVE
                     KSMYKHHADLQKTNTNIANRYSASANEYAAQAKSLGSLPKSVGYAALKNLGNALAALQ
                     MGEALYSNKANNIAKTGSSILGGMLAVAAAGAIASLAGVTLPFWASIAVGASIGLGIS
                     AAWDAFGVGEKLGLDPNVPAFDPFFGWLYDKTGGWEGGIPDWAKEWLGLNRDGKYHQY
                     DPLTLD"
BASE COUNT         1645 a         1261 c         1243 g         1437 t
ORIGIN      
        1 ccgcggtggt ctcggacgta acggattctt tgacggttca ggtattgttc gattggttgc
       61 caatcaatca cctggtccaa cttcaatagc gggaagcggt cgatgtgttt ggcaatcatg
      121 gcttgtgcgg tttgctgaaa gaaggtgctc atggaaaatc ccctaaatgg cttggtggga
      181 atttagggga ttttggggaa ttttgcaaag gtctctagat gagtgaaaaa gaagtgcagg
      241 ctgcctaaaa agacagaaaa agtctttccg gcagcctgca ctttggtttc atttcagtca
      301 gtaaacccag taaacgacgg tctaaaaacg cagaacgtta cgaaaaaagc agcctacacg
      361 cccatccccc gccttctacc cgttctgtaa atcatacaga tagcggtaat atccgttcgg
      421 cttcgccagc aattcctgct gtgttcccgc ttccacaatc ctgcctttat ccatggcaat
      481 gatccggtgt gccgttttaa cggtggacag acggtgggcg ataatcagca ccgtccggtt
      541 ggcgcaaatg gcctgcatgt tctgcataat cgctcgttca ctttcataat ccagcgcgct
      601 ggtggcttcg tcaaaaatca gaatgcgcgg attggtgatt aacgcgcggg caatcgcaat
      661 acgctgccgc tgtccgcccg acaagccggc cccttgttcg cccaccacgg tgccgtagcc
      721 ttccggcagc tccataataa actcgtgtgc gcccgccagt ttggctgctt cgataatgcg
      781 ttccagcggc atacccgtat ccgtcagcgc gatattgtcg cgtatgctgc ggttgagcag
      841 cacattctcc tgcaagacca cgccgacctg ccgccgcagc caggcaggag cggccaaagc
      901 caaatcgttg ccgtccacca acacccgtcc ctgctccggt acatacagac gctgcaccaa
      961 tttggtgagt gtggatttgc ccgaccccga acgtcccaca atccccagca cttcccccgc
     1021 ccgaatccgc aaatcagcct gccgtccgcc ttatagcgga aatcgacatg ttcgaacgta
     1081 atctcccccc ggatatcggg caaagccaaa tgcgaagacg cattctcggt cggcgcattc
     1141 agaatatccc ccaaacgcgc caccgaaatc cccacctgct ggaaatcctg ccacaactgc
     1201 gccaaacgga taacaggcgc cgccacctgt cccgagagca tattaaacgc aatcagctgc
     1261 cccaccgtca gcttgccctc aattaccagc cgtgcgccaa tccacaacgt cgccaccgtc
     1321 accagcttct gaatcagctg caccccctgc tggccgacca ccgccaactt cgttacccga
     1381 aatcccgaag ccacataagc cgccaactga ttgtcccaac gctgcgtcat ctgcggctcc
     1441 accgccatcg cctttaccgt acccaccgca gtgatgcttt ctactaaaaa cgactggttg
     1501 tctgcattgc gcgcgaactt atcgttcaga cgcgtccgca gtatcggact gataaatgcc
     1561 gaccaaaacg cataggcagg caacgaagcc aataccaccc aagtcagagt ggagctgtaa
     1621 taccacatca ccgccagaaa gataaacgaa aacgccaaat ccaacaccga agtcagcgcc
     1681 tgaccggtca agaaattgcg aatctgctcc aattcccgca cccgagccac cgtatcaccc
     1741 actcgtctgt gctcgaaata ggataaaggc agggaaagca gatgccggaa caaacgcgcg
     1801 cccaattcca catcaatacg tgaagtcgta tgtgcaaaca gatacgtccg caaaccgccc
     1861 aacacaatct caaacagcga caccaccaac aaagccaccg acaccacatc caaagtagag
     1921 aatccccgat gtaccagcac cttgtccatc accacttgga aaaacagagg cgtaatcagc
     1981 gcaaacagct gcaacaccac cgacaccacc aatacttcaa aaaacaaccg gcggtatttg
     2041 attaccgccg gaataaacca ggtaaagtca aactttgcca aactgcccaa taccgaagcg
     2101 cgggaagcaa ccaatatcag tttgcccgaa tatctgttag aaaattcggc aaaagacaat
     2161 accgcagact tattcgtaac caaatcctgt atcaaaaatt gggcatgctc accctcaccg
     2221 tctgttttgg ccaaaatgaa atggttgccg tcatcacacc ataccaatgc gggtaaagtc
     2281 gccatagcca aacgtttaat aggctggcgg actacctttg ccttcaatcc caaagatttg
     2341 gcggctaaca gccattgcgt ttcatttaaa tcgctctgtg cggaagtaca aaattcatgc
     2401 tgtatatcgg caggattggc ggcaatgccg tggtaatggg cgaggatgat gagggcggaa
     2461 agggcgggga gcggtgcgga tacgatagac ataaataaaa tatagttaga ttggatgtgg
     2521 ataacggctg gctggaaaag gaatatatta agtagaaaga aatatataaa taaaacagca
     2581 gaacgcattg taaggatata tatgggaatt gtaaagagaa agtatggaaa agttctcgtt
     2641 tcaggaaggt aaaacggctt aggaatcgag ttagatgagg atgcctcgca cctctcgtgc
     2701 ctcctgcata ccgttaagga acagggttaa ggtgcaggct gctccgaact ctgttgcggt
     2761 cggataatgt tattttttgt gtttcagtca gcctgaaata tctgtatatt tttgttttaa
     2821 atagatttta aagattgata actgttcttg acgatttttc aagaaaggag taaagtgact
     2881 tattatcaat gacaagcaac gcgcgaagtg acaaggaaaa ctatctactt aaattctaag
     2941 gaggcttcga atatcataaa ccaatcagaa acatagagat aaaaattatg tacaaatata
     3001 atcctcttat acaatttatt gcacagttga ttatgtctta tggagcaagc atagggtggg
     3061 cacttgctgc ccacgcgttt catatttcaa ggcagcctga aactgtgtgg gcataatgcc
     3121 taccctacat cccaaaaaaa caagcgcagc ctgcgtgtgt agggtgcgaa ctttcggcag
     3181 gcagacacgc ggttttatag tttcaagctg agttgggatg cttaagataa agtacaaaac
     3241 cttaaaaaat aagggggctg tactagatta gccctaaatt ccacaccaat cccgcaaaat
     3301 ttttagctgt cgggacggtg tgccgaagtt aaaccgaaat ttgcattctt tcaagaacag
     3361 cgggaaagat ttacgatcaa tttcgttgta tttgcgcaag gcgcgtttcg cctgattcca
     3421 aaagttttca atgccgttaa tgtggttctg acggtctgca aactccttag aatggttgat
     3481 gcggtgatgg ataaaaccgc ttacgtgcag cttgtcatag ctgctcagac tatcggtata
     3541 aacaatactg tccggcatga ttttcttctt gatgacaggg agtaacgttt cagacttggc
     3601 attatccacc acaacggtat agacccgtcc gttgcgtttc agaatgccga agacaaccac
     3661 ttttcctgct gcaccgcgac cacgtctacc tttacgccgt ccgccgaaat cgctttcgtc
     3721 cggctcgaca gggccctcaa aaacctcatc ggcagccaag gccggataat ggctgatgac
     3781 catacggatt gtgcggtaga acagaatagc ggagtcgggc tggatgccca aaatatcggc
     3841 agctgaacgg gcggtaactt caagtacaaa aaagcggagc agttctttct gtactttttt
     3901 ctttaatttg cagtgtgttg tcttcatatt tcgagggtaa catatctgct aatctagtac
     3961 agcctcaaac aaaaaagaga aattttaatt tcgctaaatc gcataaagat taatcaagag
     4021 tatcattaaa tgatatgagt gagcatctat aatgccaaga agagttgtta agacataatg
     4081 attattgaaa tagattgtaa aatagatact tagatagtct gaaaaacgga tttgtgaaac
     4141 tttttattac gcgccatcat ttgaaaatga aacttaaaaa acacttatca taataaatat
     4201 tttctttacg ttgtttgcta ataaactcag tgcaatatca tcgcaatatt ttatggaaat
     4261 tttatggata acaaaaaaga atttattaat aatttaacaa ataggtatat gtggatctat
     4321 ccattggtct taaatattct atttctacct ttttaccagc cctaccaatc tttttttatt
     4381 gcgcttggtt gtttgtttgc actggttaga aaaatgcaaa gcttagattt taaattacaa
     4441 aatcatattg tattgttaaa tataaaaagt gcttgggcag ataaaaaagt atttttgatt
     4501 aggatagtag tgtcatggtt ggcggtaatg gaaatatgga tgtgttttat ttcggaatca
     4561 tcaacgtggg tatgcggtgc tttttgttta aatagtgaaa tattggaaaa aatttttcgt
     4621 ggctttggtt attctggtag tttatatttt ttatttatat tgatgattga tctcaataag
     4681 ttaagagaga gtatttgaat ttcatttttt gtttgactta aactcaagga gaataacaat
     4741 gattggtagt ggtgatacta aacaatgcaa aaaattttct gcggatgagt ttgcaaaaac
     4801 aatccaaagc aactttcaat caggtattaa atatgatggg ccgactttgg caacggcagg
     4861 cgtatctaat ataatagatg aatacttaaa ggatgcttat ggcggaaatg ggttaaaaga
     4921 agcgcaacgc aatcttgtag ctaaagagat tgcggataga atagaggcag aattgcatag
     4981 gcagcgtgct aattatcaag cacaagaagc tgcaaaatat tctcctgcgc atggcggagt
     5041 ggagaaaagt atgtacaagc atcatgctga tcttcaaaaa acaaatacta atattgctaa
     5101 tcgttattct gcaagtgcta atgaatatgc ggctcaagca aaatcattag gaagtttgcc
     5161 aaaatcagtt ggctatgctg cactaaaaaa tttgggaaat gctttggcgg cgttgcaaat
     5221 gggagaagct ctatactcaa ataaagctaa taatatagcc aaaacaggct cttctatttt
     5281 aggtggtatg ttagcggttg cagcagcagg tgctatagct agtttagcag gagttacatt
     5341 gcctttttgg gcttctattg cagttggtgc atctattggt ttaggtatta gtgcagcatg
     5401 ggatgctttt ggtgttgggg aaaaattagg attagaccct aatgtgccag cttttgatcc
     5461 attcttcggt tggctatacg ataaaacagg cggttgggaa ggaggtattc ctgattgggc
     5521 taaagaatgg ttgggattaa accgcgatgg caaataccac caatacgacc cactaaccct
     5581 agactt
//
LOCUS       DAXHKX010000133         5550 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHKX010000133 DAXHKX010000000
VERSION     DAXHKX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.133"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(42..809)
                     /locus_tag="ACFMWZ_001664"
     CDS             complement(42..809)
                     /locus_tag="ACFMWZ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC5935596.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALAALDIGLVVGKPAPEHLDGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(897..1724)
                     /gene="mutM"
                     /locus_tag="ACFMWZ_001665"
     CDS             complement(897..1724)
                     /gene="mutM"
                     /locus_tag="ACFMWZ_001665"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC5935597.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1776..2441)
                     /locus_tag="ACFMWZ_001666"
     CDS             complement(1776..2441)
                     /locus_tag="ACFMWZ_001666"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC5935598.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     IAD"
     gene            2620..4596
                     /locus_tag="ACFMWZ_001667"
     CDS             2620..4596
                     /locus_tag="ACFMWZ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC5935599.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAIEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQMASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPRNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAVRFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4664..5356
                     /locus_tag="ACFMWZ_001668"
     CDS             4664..5356
                     /locus_tag="ACFMWZ_001668"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC5935600.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGCVSINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVIPEPYFYILLNKPEDYETSHKPKHYRSIFSLFPDNIRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
BASE COUNT         1469 a         1778 c         1184 g         1119 t
ORIGIN      
        1 ccacaaaagc gaatcatact gccacattcc gacaaccggt ttcagacagc gatatccgca
       61 tcgtcggcga caacggcacg gatgctttct tcgattttat cttttaaagc ataacggtct
      121 tcgctttccg ccgcatccgc cacgcaaacg aaatcgactt ttatcgtcaa ttttttcata
      181 gacacgatgc gccacaggca ggtcggcaaa ccgacatcgg catatgaggg acgagccgtc
      241 ctttttcccg tttcgtcata ataacgcagc gcgaccgcca aaacctttgc ccccgcatcg
      301 atggcggatt ggaacagcgc ggctttgaac ggcaaaagcc ccaatccgga ggaagtccgc
      361 gcttcgggga aaaaactgac gttttgaccg cgttgcaagg tttcgcagac ggcgcggtta
      421 atcggttcga tgtcgcgccg cgaattgcgg ttgatgaaca ccgttcccgc gttctgcccc
      481 atcttgccca ataccggcca gcttttgatt tcctgcttgg cgataaagct gctcggataa
      541 accgcgctca tcgcgaaaat atccagccag gatacatgat tggcagctac caagactccg
      601 tccaaatgtt cgggtgcagg cttacctact accaatccga tatccaaagc cgccagagcc
      661 cccctgccca actcgattac cgcccgattg cgcgactcgg gacagccgcc gtcaataccg
      721 cgcaggtttt tgccggtttt gaatagccag accgtcaagc ggcacaagcg gcgcagacgt
      781 gtaaaaaatg aagctttatt tgaagacatg aacaattctt tcccgataat cggtgtttgt
      841 tgttgaaacg ttcggacggc ataccgataa aatgccgtct gaaaccgttt tcagccctat
      901 ttctgacagt tcgggcaata aaacgtgccg cgctgcccca aagtttcttt cacaaccaaa
      961 ccgccgcact gggggcacgg ctgattgtgc cgcccgtaca ccgtatattc ctgttggaag
     1021 tagccacttt tgccgtcgct gtccacaaaa tccctcaagg tactgccgcc cgtttcaatg
     1081 gcgcgccgca acaccgcttt gacggtttca accaaaagcg cacactcttt ctttttcaga
     1141 cggttggcag gacggtgggg cgaaatgccc gctctgaaca ggctctcgtt ggcataaatg
     1201 ttgcccacac cgaccacgac cgcattgtcc atcagggcaa gtttgaccgc gcgtttctgc
     1261 gccttcagcc tcacatacag ataatccgca caaaatgcct ccgacaaagg ctccggcccc
     1321 agtttttcca acagcggatg atgttcttcg attccctcat accaaagtat cgcgccgaac
     1381 tttctcggat cgcggtaacg catgaccgtg ccgtctgaaa acacaatatc gacgtgatcg
     1441 tgtctgtccg gcctgccgat acgtccgtcc gacggcgtaa aaatccgcaa gctgcccgac
     1501 atccccaagt gaatcagcaa aatccccgtt tggaaacgga caatcagata tttggcacgc
     1561 ctgccgcagg acaaaacctg ccgaccggac aaaatctccc ccaaatcggg attgatctgc
     1621 cagcgcagct tcaattggcg caataccacg gcttccaccg ttttcccttc aatatgcggc
     1681 gcgatgccgc gcaacgtcgt ttccacttcc ggcaattccg gcataacccc tcccgacatt
     1741 tcttctgaca gatgccgtct gaaagacggc tgtccctaat ccgcaatcct tgccgcaccc
     1801 gccgcaaggg ctttgccgcc caaatacccg tcccacgcgg gcaggggcgt tacgcccgcc
     1861 gcccttttga ccaaaaccaa accgtacact gcggcacact gcggccacca acggtcgccc
     1921 gccttttcca taaaccgcca aaagcgtatt tgcccgagcg acgaaacggg cggcagatac
     1981 accataaatt tcccaaattc aatatcgaaa ccgacatccg caagctgcct tttcaactcg
     2041 ggcagcggca gacaaaaccg tttttccggc aggcgttcgc cgtcaaacca acggctgaat
     2101 ccccagagcg aatacggatt gaaacccgtc agcatcaaac gtccgcacgg tttcaatatc
     2161 cggtgcgctt ccgacaggat ttgcgaagga acaccgcctt ccagagtatg cgggaaaagc
     2221 agcatatccg cagaaacatc cgccaaagcc atattttccg ccgacatcga catatctcgc
     2281 ggcacacaga caacatcttc agacaggctc agccacggac cgcccacctg aaccgcacac
     2341 attcccgaaa aacggtatga atccagatac cgcccgaaga aatcctgttc caattttgca
     2401 acataccgcc ccatcgccgt atcttcaaac catgaatcca tattgcgtcc gtttcaaaca
     2461 gattgcctgc cgatattcta ttccaaacag gatttctgtc aaaaaacaca tcggccgccc
     2521 atttccgaat ccgcataaag ttcctgtaaa acttgacgct ttttcagtca aacagtacca
     2581 tcggacgata aaatatgttt attccgccga atataaatca tgtccaaact caaaaccatc
     2641 gctctgaccg catcaggtct gtccgtttgt ccgggtttcc tatacgccca aaacacctca
     2701 tcacaccaaa tcggtttggc gattatgcgc ttaaactctt caatactcga cctgcccccg
     2761 acaaaacaat atttccaatc cggcagcctg tggagcgagc tgcgccaagg cttccggatg
     2821 ggcgaagtca atcccgaact ggtacgccgc cacgaaagca aattcatcgc aagccacagc
     2881 tatttcaaca gggtcatcaa ccggagtaga ccctatatgt accatatcgc caacgaagtc
     2941 aaaaaacgca atatgcccgc cgaagccgcc ctgcttccct tcatcgaaag cgcgttcgtc
     3001 accaaagcca aatcacacgt cggcgcatca ggattatggc agtttatgcc cgctaccggc
     3061 aggcattacg gcctggaaaa aacaccggtt tacgacggca ggcacgacgt ttacgccgcc
     3121 accgatgccg cactcaacta tctgcaatac ctctatggac tgttcggcga ctggccgctt
     3181 gcctttgccg cctacaactg gggtgaaggc aacgtcggac gcgccatcaa ccgcgcccgc
     3241 gcccaagggc tcgaaccgac ctacgaaaac ctgcgtatgc ccaacgaaac gcgcaactat
     3301 gtccccaagc tgctcgccgt gcgcaacatt attgccactc cccaatcttt cggcatgaat
     3361 atcagcgaca tagacaacaa accctatttt caggcaatcg aaccggaccg tccgctcgac
     3421 aacgaagcca tcgcccggct tgccggcatc acgcaaagcg agctgctcgc cctaaacccc
     3481 gcattcaacg tccccgcgtt catccccaaa agcaaacgca aactgctgct tcctgtcgca
     3541 tccgtccaaa ccttccaaag caactacctc aacgccgcac ccgacagcct gttttcatgg
     3601 gaagtctata cgcctgccgc caaaaccagc ctgtccgaca tctcgacggc aaccggcatg
     3661 agcattgccg acatcaaacg cctcaacaac ctgaacggca accttgtcaa cgcaggacgc
     3721 agcatccttg tcgccaagaa cggcaagacc cttcagatgg catcggaatc cgtcgtttcc
     3781 atcgacatcg acaatacgcc cgacacctac cgttccaata tgccggcagg cacggtgaac
     3841 gtcggcattg cccgaatccg acccgccgcc gcacagacag cggacattac cgtcgcacct
     3901 ttgccgcaga aaaccgtccg tacggaaccc gatccccttg tccgtattgc cgaacctgcc
     3961 cttgcgacag ccgcagcgca acctcaaacc gaaaaacaga ccgccatgcc gtctgaaacc
     4021 caaaccgcaa cactcgcgca gatcatcccc cgaaacgaca tgcaggcggc agacgaactc
     4081 atgcagcttg ttgcccgaaa caacctgcgc cgacaggctg aagaaaccat ctccgccgtc
     4141 atcggcacgc ctgacacagt tgccgaacac aacatttcct catctccgca acataccgtt
     4201 gccgccgacg gcaaacgccg ggcacgtttg gaaacgcgcg tagccaaagc tgccgacggc
     4261 gaagccgaaa tctccccgct ccatgccagc atccaccgcg ttgtagaagg cgacaccctg
     4321 ttcaacattg ccaaacgcta caacgtcagc gtagccgacc tgattgtcgc caacaacatc
     4381 aaaggcaaca ccatccaaaa aggacaggta ctccgcctgg cgcaggcagc ccctgcccaa
     4441 acccgtattg aaaaagtatc ctacaccgcg cgcaaaggcg acaccttcaa aagtatcgcc
     4501 gtgcgcttca atatccatat cgacgacatc cgccggctca atcccaacct gaacaccatc
     4561 aatccgggac agagggtcaa actgattgga agctgattcg gatacggcat atgacagaac
     4621 tttcgcagtc ctgtcttttt catcccacat ttcccatacc atcatgaaac tcatcaaata
     4681 cctgcaatat caaggcatag gaagccgcaa gcagtgccaa tggctgatta ggggcggctg
     4741 cgtctccatc aacggaacct gcatggacga caccgatgca gacatcgatt cctcatccgt
     4801 cgaaacgttg gatattgacg gggaagcagt aaccgttatt cccgaaccct atttctacat
     4861 cctgctcaac aagcctgaag attacgaaac ttcacacaaa ccgaagcact accgcagcat
     4921 attcagcctg ttccccgaca atatacgcaa tattgacatg caggcagtcg gcaggctgga
     4981 tgcagataca accggcgtat tactgattac caacgacggc aaactgaacc acaacctgac
     5041 ttcgccgagc agaaaaattc ccaagctata cgaagtaacg ctcaaacacc ccacaggaga
     5101 aacgctctgc gaaaccttga aaaacggcgt gctgctccac gacgaaaacg aaaccgtttg
     5161 tgccgccgat gccgttttgg aaaacccgac caccctgctg ctgaccatta ccgaaggaaa
     5221 ataccaccaa gtcaaacgca tgatcgccgc cgccggcaac cgcgtgcaac accttcatcg
     5281 ccggcgattc gcacatttgg aaacagaaaa cctgaaaccc ggcgaatgga aatttatcga
     5341 ttgtccgaaa ttctaaaata acatccaaga attccattta tattcatcta catactcatt
     5401 aaatatatgg tttaacccaa tttaacgcaa aaaatcaatt tacgatataa tccatttgtc
     5461 tgagtaacgc ctgttcaagc aggcttatga gtaagacgtt ttccccgtaa tgtgttttgc
     5521 catctatact tctccccttg tatagatggt
//
LOCUS       DAXHKX010000134         5532 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHKX010000134 DAXHKX010000000
VERSION     DAXHKX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.134"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(241..3003)
                     /gene="tbpA"
                     /locus_tag="ACFMWZ_001669"
     CDS             complement(241..3003)
                     /gene="tbpA"
                     /locus_tag="ACFMWZ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC5935601.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDGSEYAYFIVEEECKSKNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLSDPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGSIRGHGKYAGDHKYSGLITNGDNGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPSSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYYQHANRAYSSNTPPKNGNGGSNVNPNANPNGGKDKPYWVSIGGGNVVTGQI
                     CLFGNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTG
                     THRTLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFN
                     KEAGIVFKGDFGNLEASWFNNAYRDLIVRGYEAQIKDGKETAKGDPAYLNAQSARITG
                     INILGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVV
                     GLGYDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVS
                     GYYTIKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTF
                     SLEMKF"
     gene            complement(3090..5255)
                     /locus_tag="ACFMWZ_001670"
     CDS             complement(3090..5255)
                     /locus_tag="ACFMWZ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC5935602.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNRHPTAMPKENEVKLKNDDWEATGLPTEPKKL
                     PLKQESVISNVQTDNGDNNIYISPYLTQSSHNSHNGSINGGANQPKNEATGYNNFKYV
                     YSGWFYKHAASETKFNEGKFKSGDDGYIFYHGKDPSRQLPASGKVTYKGVWHFVTDTK
                     LGQKFNDIIQPSKKQGDSYSGFSGDEGEEYSNKNESTLKDGHEGYGFTSNLEVDFGNK
                     KLTGKLIRNNASQSNTNNDKPTTQYYSLEAQVTGNRFNGKAIATDKPQENETKQHPFV
                     SDSSSLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKPRNGAVASGGTGAAASDG
                     AAGTSSKNGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGKN
                     QANQGTNGGTAFTYKTTYTLESDKKDTKAQTGAGGAQAASGAAGVNGGQAGTKTYEVE
                     VCCSNLNYLKYGMLTRKNSKSAMQAGENGSQADAKTEQVEQSMFLQGERTDEKEIPKE
                     QQDIVYRGSWYGHIANNTSTSWSGNASNATSGNRADFTVNFGDKKITGTLTANDRTQP
                     TFTITANIKDNGFEGTAKTAELGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGG
                     WFAYSGDKQTENTTVASGNGNSASSATVVFGAKRQQPVQ"
BASE COUNT         1140 a         1393 c         1397 g         1602 t
ORIGIN      
        1 ctttcgcggg aatgacggga tttgaggttt ctgtttttga ttttctgttt tcgcgggaat
       61 gacggtttag aagttacccg aaacctcaaa aaaacctaaa ccgaacggtc tagattcctg
      121 cctgcgcgga aatgacgggt ttcaagatta cggtgttgtc ggggatgatg ggaaacggtg
      181 ggaattgtgt aaaaaatgcc gtctgaagcc tttcagacgg catttgcggc gtttggacgt
      241 ttaaaacttc atttccaagc taaatgtgta gtttcggccg ggggcggcat atcggttgta
      301 aacgccgaca tttttgtgtt ggttgactgc gccgccggca gtttgccgca cattttccca
      361 agtaacatag cggtagttga ggaggttgta cacgcccgca cggagggtga agtgtttttt
      421 aatcgtgtaa taaccggaca catccacaat ataccaaggg cgggtacggc gcgcggtggc
      481 ttttgtattg cggctgttgc cgttgagcaa agcccggctg cccaacaact ctgtgatttc
      541 cttggctttg gaataagtca gcataccgtt cacaccccat ttgccttccg gttggtcata
      601 gcccaagccg acgacatagc gcgaaggttg gatggcatca aacagatgtg attgaatatc
      661 ggtgcggtct gcgcgttttt tgatgtcgcg gacacggaca cgattatagg caaatgtaga
      721 ataccaacct tcgggcaatt tatcccatac gccgttccaa tcgattttgc ccaaaatatt
      781 gatgccggta atccgcgcgc tttgggcatt gagataagcc gggtcgcctt tggctgtttc
      841 tttgccgtct ttaatttgcg cttcataacc ccggacaatc aaatcgcggt aggcattgtt
      901 gaaccaactt gcctccaagt tgccgaaatc gcctttaaac acgatgccgg cttctttgtt
      961 gaacgatttt tccggatcga ttttgaccgc cttgctttga acacccgccc gccagccgta
     1021 catttccgca aacgagggca ggcggaagcc ggttgaagtg cggtaagtca aatccagcca
     1081 gtcggcaggt ttgaggacga tgcctgcgtt ccaggacagg gtacggtgcg tgccggtgga
     1141 aacgctgccg tcgtccgaat gcgtgctgcg gtagtcgtag cgcaagcccg cgccgacatc
     1201 cgcccacctg cccaaacgga cattgtcccg aactgccgcg taatagcttt tgccgttgat
     1261 gctgcgcggc gtgcagtccg tataagtatt gttgccaaag aggcagattt gccccgtaac
     1321 cacatttccc ccgcctatgc tgacccaata gggtttgtcc ttgccgccgt tggggttggc
     1381 gttggggttg acgtttgagc cgccgttgcc gtttttagga ggcgtgttcg acgaataggc
     1441 gcggttggca tgttgataat aataatcctg atggcggaga ttagagccaa agcggtcaaa
     1501 cccgagattc acgctcaggt tgtggcggat tttggcggta tcgaaggatt ttttgaatgc
     1561 cgcctgcaag agcctgtggc tttccccgta aatcacgcga tcggatttgt agtaggaaga
     1621 cggcttgtcg gcactcgggc ggcaatattt gtccgaaccg tcggcagaac agtgcgtctg
     1681 ctgaaaatga ttgtccaaac cgatgccctg acggtcgtaa gagaggcggg cataatccgc
     1741 ccaagtgtct ttatcggcat tggtatagac atattccaaa ccgtagcggc ttttggtgtg
     1801 cgtctcgtcg taaaacacac ccgtaccgta ttccgcgccc accagcgcac cgttgtcgcc
     1861 gttggtaatc agcccgctgt atttgtggtc gcccgcgtat ttgccgtggc ctcttatcga
     1921 tccgtatttt ctattttcat caaaaaccgc cttggtcagg aatgccggaa ccgtcatatc
     1981 gcgcgtgtcg aaagtttgtt gcgtgtgttc gagtatgccg ccgatgtagt gccgtttgtt
     2041 ttcaaaacga aaacccgggc ggaacagcca cgaccggctt tcgtatgaaa gcggatcgga
     2101 gagaaagcgg ttgggacccg tgtagtctcg ggtggaaacc gtttgacgtt cgtctttgcc
     2161 gacaacatct tttttcggat tcgctttaca cgtttcgtaa tttttgcttt tacattcttc
     2221 ttcaacgatg aaataggcgt attcgctgcc gtcttcaacc ggcaccagcc tgttaaagct
     2281 ctgaacgccg cgtccggcgg cttcgtgggc gcggatttcc cccgcgcgcc ggccggtgtg
     2341 gatcagcaaa gcctccgcac cgccgatgcg ccccgccagc gcgatggatt gggtaagccc
     2401 ccggtttttg ccggaatagg cggttttact ctgaatgccc cactgcctgc cttccccgat
     2461 aacatcgtcg gcggttttgg tttgaaatgc gaccgaaccc gccaatgcgc cgctgccttg
     2521 ttcgaccgag tttgagcctt tgctgatttc gacagctttg acgttttcat actcgatttc
     2581 attgattgcg ccgctgctgc ccgccgtcct cgtcccgccc aatgccgcct gagcggtgta
     2641 ggactgtatt tgcgccaagc cgtccaccgt caaggaaacg cggtttttgt ccataccgcg
     2701 tatcgagtag cccgagcttg cgccgcgccc ctgttcgacg acggcgatgc cggggtcgta
     2761 acgcgtcagg tcgcggatat cgagtacctg ttccttgctg agggtgtcgg cggttttgac
     2821 caatttgccc aaaccggtga cttcgttatc gcggcgggtt ttctgttttt tggcttttac
     2881 ctgtatggta tccaactgtt tttcctgtgc ttgtccggct tgcacatttt ctgcataaac
     2941 gggcagcgcg gtcatcagcg acaggcataa aatatttaat cggaacaaat gttgctgttg
     3001 catagtgttt ccctactctt cgctttcaga cggcatcgga aggagcggtg ccgtctgaag
     3061 ccttattctt gattgttcgg caaccgtgct tattgcacag gctgttggcg tttcgcaccg
     3121 aatacgacag ttgcactgct tgctgaattt ccattgccgg atgcaactgt tgtattttcc
     3181 gtttgtttat cgcccgaata ggcaaaccat ccgcccaact cttcggcttt aggtccgtaa
     3241 aaaccgccct gcaccttggc gtttgtgata tatgccttag gcgtgccggt ggtattgctt
     3301 tgatcgagat caaaacctaa ttcagcagtt tttgccgtac cttcaaagcc gttgtcctta
     3361 atattagccg tgatagtaaa ggttggctga gtcctatcat tagcggttaa cgtgccggta
     3421 atttttttat cgccaaaatt cacggtaaag tccgccctgt tgccactcgt tgcattggaa
     3481 gcattgccgc tccagcttgt gcttgtgttg ttggcaatat gcccgtacca agacccccga
     3541 taaacgatgt cttgttgctc ttttggaatc tctttttcat cggtgcgctc gccttggagg
     3601 aacatacttt gttcaacttg ttccgtttta gcgtcagctt gactaccgtt ttcgcctgcc
     3661 tgcatcgcgg acttgctgtt tttacgcgtc aacattccgt atttcagata attgaggttg
     3721 gaacagcaga cttcaacttc ataggttttt gttcctgcct gcccgccgtt aacgcccgcc
     3781 gcacccgaag cagcttgcgc gccgcccgcg cctgtttggg ctttggtgtc ttttttatca
     3841 ctttccagcg tgtaggttgt tttgtaggta aaggctgttc cgccgtttgt accttgattg
     3901 gcttgatttt tcccactttc ggaagcctcg ggcaggagcg gaatcataat gccgtcgaca
     3961 accagttggg cggcattgct gaagttgtcg agatttttga ttgctgtgcc gccgtgcgtc
     4021 agctcgaccg catccaaaac cgtggtcagc ttaccgtttt tagacgacgt gcctgccgca
     4081 ccgtccgatg ctgccgcacc tgtgccgcct gaagccactg cgccatttct cggtttgtct
     4141 tgggttttcg cgctgccgac aacggcaact ttttgatcgt tgctcaaaaa gcggaaaccc
     4201 aattcctcac cctgcgggcc gaaaaagccg ccgctcaaag aagacgagtc ggaaacaaag
     4261 ggatgttgtt tggtttcatt ctcttgtggt ttgtcggtcg ctattgcctt gccattgaag
     4321 cggttgcctg ttacttgagc ctcaaggctg tagtattgcg tggtaggttt gtcgttatta
     4381 gtattacttt ggctcgcatt attgcgtatc aatttacccg tcaatttctt attgccgaaa
     4441 tccacttcta aattcgaggt aaatccataa ccctcgtgac catctttcag cgtggattcg
     4501 tttttgttgg aatattcttc gccctcatca ccagaaaatc cgctgtagct gtcgccttgc
     4561 ttttttgaag gctggataat atcgttaaat ttttgtccca gtttcgtatc ggttacaaaa
     4621 tgccatacgc ccttgtaggt aacttttcca gaagcaggaa gttgtcggga agggtcttta
     4681 ccgtgataaa agatatagcc gtcgtcgcct gacttaaatt ttccttcatt gaattttgtt
     4741 tcactcgccg catgtttata aaaccaaccg gaataaacat atttgaaatt gttgtaacct
     4801 gttgcttcgt tttttggttg gtttgcaccg ccattgatac tgccgttgtg agaattatgg
     4861 cttgattgcg tgagataagg ggaaatgtaa atattgttgt cgccattatc tgtttgtacg
     4921 tttgaaatga cggattcttg ttttaatggc agtttcttgg gttctgtcgg caatcctgtc
     4981 gcctcccaat catcattctt taatttaacc tcattttctt taggcattgc cgtaggatgc
     5041 cgattcctcc gtttcaacct catcgcaaaa ccgtatccgc cttggtcttt ttgggcttgc
     5101 ggtttttcgg aagaaacatc ttgatacttt ggcgcgggac gcggggcttc ggtatcgaca
     5161 gaatcaagat cgaagctgcc gccgccgccc aaacaagcac tcaacaaaaa cacaggcagc
     5221 accatagcag cctgatttac caatggattg ttcatataaa cccaattcaa ttaaagaatg
     5281 ataaggatta ttattttatt tatttttaac aaatttgcaa atactttttt atttttttag
     5341 ggaatacacc aaaatcccgt cattcccgcg caggcgggaa tccgaacgcg tccgcacgga
     5401 aacctatatc ccgtcattcc cacgtatatc ccgtcattcc cgcgcaggcg ggaatctagt
     5461 gcgttgagtt tcagctattt agaataaatt ttgaaactct aatcgcgtca ttcccacgaa
     5521 agtgggaatc ca
//
LOCUS       DAXHKX010000135         5468 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHKX010000135 DAXHKX010000000
VERSION     DAXHKX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.135"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            131..565
                     /locus_tag="ACFMWZ_001671"
     CDS             131..565
                     /locus_tag="ACFMWZ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC5935603.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="ACFMWZ_001672"
     CDS             complement(578..1441)
                     /locus_tag="ACFMWZ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC5935604.1"
                     /translation="MELDEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="ACFMWZ_001673"
     CDS             complement(1449..2066)
                     /locus_tag="ACFMWZ_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC5935605.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2700)
                     /locus_tag="ACFMWZ_001674"
                     /pseudo
     CDS             complement(2229..2700)
                     /locus_tag="ACFMWZ_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            2823..4595
                     /locus_tag="ACFMWZ_001675"
     CDS             2823..4595
                     /locus_tag="ACFMWZ_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC5935606.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4592..5248
                     /locus_tag="ACFMWZ_001676"
     CDS             4592..5248
                     /locus_tag="ACFMWZ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC5935607.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1340 a         1547 c         1275 g         1306 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gtcagctttc ccacgccgcc acggcttata tccccccgaa
      241 cgattttcaa ccgaactgcg acatacgccg actcgggctg acccaaagtc agcacaatga
      301 gctgcgtaaa atccgcaccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcctcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accggttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ctccgcgcac gcctgtcgta cctgcatttt catccaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtacacc agcacgttgg cgcaacgttg ggacagaccg ccgtccaatg
      961 ccgttttcaa tgccttatag gtttcgtcgc cgctttcaaa tgacaaatcg tcgcaaaata
     1021 caatgaattt ttccggacgt tccttcaaaa gcgtcaacag atacggcagg ccgatcaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgc agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacgtta ttcgcgggtc
     1201 tgccggcaat gaattgttcg gtattacgca ccagcaattc ggtttgcctg ccaactcccg
     1261 ccagccttgc caaggggaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gagaaattcg tccaattcca
     1441 tctctacctc actttgcata tctttgcgcc atcagccgtt cgacggtatc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatt ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcaaatgg acattgaaac ggctgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcgtgcggga gcgaaaacca aaccgtgcgg ttgtacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccctaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tgttggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtctg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aagccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtacggc ttgacctttc ctttccacgc cggtcggcgg
     2221 tcttaccctt atccctcctg caaatcggtt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccgaccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa accttgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cagcagtcgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgtgcgtcc ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggca aataaattca tattatctga attttatcat
     2701 aacatgattt aacactgaag cggtgcggat taagttttcc ttaaccaatt ctttctgagc
     2761 agttgtatct aatcccaaag aatgatattg tttgcattat ttagaacaat ttttcgccga
     2821 gcatgatact gccagcccgt ttttcagacg gcatcagcct ttccctacgc ctgaaactcc
     2881 tgaccggact gtgggtcggg ttggcggcat tgtctgtcgt tttgacactg ctgctctctt
     2941 tgcgtctgga aaacgcggcc tccgtcatcg aagaggcggg caacttgaga atgcaggcat
     3001 accgtctggc atacatggcg ggtgaaggct cgccccgtgc gcaaattgac aatcaggttg
     3061 ccgaatttga aaaaagcctg aatcgtattt cacaaagtga tgccatccat ccgctgattc
     3121 cttcggacac ccctcttgct tatgatttga tacaatccat gctgattata gattggcagg
     3181 cacacatcct ccccccgctc cagtcctacc ggcgaccgac tcaggtcgat ctctaccgct
     3241 ttgccggaaa catcgaactg tttttgcagg cattggaaaa tgccaacgaa aaaaacacat
     3301 ggtggctcag gcgttttcaa tgggcaatta tgttgatgac gctggtgtcg tctgtactga
     3361 tgctgttttg gcaccagatt tgggttatcc ggccgctgca ggcgttaagg gaaggtgcgg
     3421 aacgcatcgg acggaggtgt ttcgatattc cggttcccga aggcggtacg ccggaattca
     3481 aacaggtcgg gcgttgtttc aatcaaatgg gcggcaggtt gaaaatttta tatgatgatt
     3541 tggaaggaca agtcgccgag cagacacgca gtctcgaaaa acaaaatcaa aacctgaccc
     3601 tgctgtacca aactacacgg gacctgcacc aatcctacat accgcaacag gctgcagaac
     3661 attttctaaa ccgtatcctg cccgccgtag gagcagattc cggcagagtt tgtttggacg
     3721 gcggatccga tgtttatgtt tccattcatc atgcggattg cggcacagca gcttcggatt
     3781 tggggaagta ccatgaggaa atcttcccca ttgagtacca gaacgaaaca ttgggcaggc
     3841 tgttgctcag ctttccaaac ggcatttctc ttgatgaaga cgaccgcatc ctgcttcaaa
     3901 cactaggcag gcaattgggc gtatcgcttg ccggcgcaaa acaggaggaa gaaaaacgcc
     3961 tgcttgcagt attgcaggaa cgcaacctga ttgcgcaagg attacatgac agcatcgcac
     4021 aagcattaac gttcctaaac ctacaggtac agatgctgga aaccgccttt gccgaaaaca
     4081 aacgggagga agccgcagaa aacatcggct tcatcaaaac aggcgtgcag gaatgttatg
     4141 aagatgtccg cgaactgctg ctcaacttcc gtaccaaaat cagcaataaa gaatttcccg
     4201 aagccgttgc cgacctattc gcccgcttta cgcaacaaac cgggataacg gtcgaaaccg
     4261 cctgggaaaa cggttcgttc ctgccgcctc aggaagcgca gctccaaatg atttttatcc
     4321 tgcaggaaag cctgtccaac atccgcaaac acgcccgcgc cacccatgta aaattcaccc
     4381 tttccgaaca cggcggacgc tttaccatga ccatccaaga caacggacaa ggtttcgaca
     4441 cggagaaaat aggagaaccc acgggcagcc atgtcggact gcacatcatg caggagcgtg
     4501 ccaaacgcat ccatgccgtt ttagaaatcc gttcccaagc tcaacaggga accaccgtct
     4561 cattgacggt tgcatctgaa gaaagcttga aatgaccatt aaaattatcc tgatagacga
     4621 ccataccctc ttccgcagcg gcattaaagc ccttttgtcg cgccaacatg attttgaggt
     4681 catcggcgaa gccgcagacg gcctctcggg cattaaaatg atcaaccggc tgcaacccga
     4741 tgtcgtcctg cttgaccttg atatgcccgg tatgaacgga cgcgaagcac tctcccaaat
     4801 catcagcatc aatccgcagc aggcagtgat tatgctgacc gtttccgaag acaacgacga
     4861 cttgaccgaa tgtatgcgca tcggcgcgcg cggctacctg ctgaaaaaca tcaacgccga
     4921 ctttctgctc gaaagcatac gcaaagccgc cgaaggcgat aatgtattct cgcccgagat
     4981 gaccgccaaa ctcgttaaaa gcctgatttc cccccaacct gcccaaggga cacaggcact
     5041 ctcctcactt acccctcgcg aactggaaat cttgggctat ctcgccgcag gacacagcaa
     5101 caaaatcatc gcccgccacc tcgatcttgc cgaatccacc gtcaaagtcc acgttcaaaa
     5161 cctgctccgc aaactcaacc tcagcagccg ggtgcaggcc gctgtttacg ccatccggca
     5221 caacgtcccc caacccgtgc tggaataagg cgggttcaga cggcatatta ggggttttaa
     5281 tccccgtacg gtcattcgga taacagacca cgcatgtaag tttatgcccc cataagtacg
     5341 cttggcatag cagtaatatt gttcggttta gtgttttccg tttgccccta tctgatactg
     5401 caatatcagc tatgccgtct gaaaacgcat catcatgata ttttcagacg gcataataaa
     5461 aagcggaa
//
LOCUS       DAXHKX010000136         5390 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHKX010000136 DAXHKX010000000
VERSION     DAXHKX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.136"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            116..637
                     /locus_tag="ACFMWZ_001677"
     CDS             116..637
                     /locus_tag="ACFMWZ_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC5935608.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYSKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMALA
                     NDGTIAGAKLVDHHEPIMLIGIPHLPAPGRAIRLNWLPAYIKPNFISTNRLRLKGLPT
                     VPQPSKETGAGVP"
     gene            526..1725
                     /locus_tag="ACFMWZ_001678"
     CDS             526..1725
                     /locus_tag="ACFMWZ_001678"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC5935609.1"
                     /translation="MASGVYQTKLYIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSADAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEISGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDELLKEVETRQSEWGR
                     AENGSLN"
     gene            1783..2397
                     /locus_tag="ACFMWZ_001679"
                     /pseudo
     CDS             1783..2397
                     /locus_tag="ACFMWZ_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2414..2545
                     /locus_tag="ACFMWZ_001680"
                     /pseudo
     CDS             2414..2545
                     /locus_tag="ACFMWZ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2542..3036
                     /locus_tag="ACFMWZ_001681"
     CDS             2542..3036
                     /locus_tag="ACFMWZ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC5935610.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3124..4785)
                     /locus_tag="ACFMWZ_001682"
     CDS             complement(3124..4785)
                     /locus_tag="ACFMWZ_001682"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC5935611.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4915..>5390)
                     /locus_tag="ACFMWZ_001683"
     CDS             complement(4915..>5390)
                     /locus_tag="ACFMWZ_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC5935612.1"
                     /translation="ASVATLLNNFYGQKLTEEDVLKKLDKEQMPASFEDMRRIMPDLG
                     FEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSMSRA
                     QFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFTRSPKRQTEFAVGQVKWWRAY"
BASE COUNT         1280 a         1462 c         1388 g         1260 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttga tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacggcgt tttttgccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacagcaagc cggaaggtaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
      361 cgatgcggtc aatacgcgcg gttattcgag caaaccgatc gatacgctga tggctttggc
      421 aaatgacggc acgatagccg gagcgaaact ggtcgatcat cacgaaccga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc ttaaactggc ttccggcgta
      541 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttcc
      601 gcaaccatcg aaggagacag gagcgggcgt accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc ctgcaatgga tgccggtatt
      721 tatctcgaag aaaccgcccc gcgcgccctg atcgaacaca acaatatttt ggataattcg
      781 gtcggcgtat atctgcatgg ttctgccgat gcgatggtgc gggagaataa aatcgtcggc
      841 gacgcgactt tgcgcgtgaa cgagcgcggc aatggcgtta ccgtttggaa cgcgcccggc
      901 gcgcaggtcg tcggcaacga tatttccaaa gggcgggacg gcattttttc caataccagc
      961 acgcacaaca cctataaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat gggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtgttt gacaatatcg ccgtcggcag ccgcgaccaa
     1141 ggcatcatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaagcg
     1201 ggcaagtgcg tttttgccta caatgccaac tacgataaac tgtccgccaa tcattttgaa
     1261 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtcact gcacgacaat
     1321 tcctttatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
     1501 gtatcgcgtc tcttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttctgcctgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 cccaaaattc aaacccgtta tcaggcgatg aaggacgagc tactcaaaga agtcgaaacg
     1681 cggcagtcgg aatggggcag ggcggaaaac ggttctttga actagtctgc ttcagacggc
     1741 atccggattc aaatgccgtc tgaaaacaca aaaggaacaa ccatgaccac acatcatgtc
     1801 gaattgagga aggtaaccaa acggttcggg gcgcaaaaag ccgtcaacca agtcgatttg
     1861 gttttgaagg caggagaaag cgtcgggctt gccggacaca acggcgcggg caagtccacc
     1921 attatgaagc tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc
     1981 gaacgtaccg gtagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa
     2041 accgttgcgc tgcacccttc gctgatcggc atcgaaacgc tggattttta tgccaaactt
     2101 aaaaaacagc cgctcacgca gaaccggggg ctgcttgagc gcgtcggcat ttcacaggcg
     2161 gcacaccgcc gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa
     2221 gccctgctgg gcgagcccaa agtcctgctg tttgatgaac cgacaaccgg tcttgaccct
     2281 gcatcacgac aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta
     2341 ttgctcagca cccacgccct tgccgagttg gacgggcacg ccgaccgcat tatcgtggat
     2401 taaatttaat ccactatatg cgggtatggc gggtttgagc ggacaaatca gcctgaccgt
     2461 ccccgttttg ctgaccgctc aggttttatg ggttatcatt ccgcttgttt tggcagccgg
     2521 aatttttaga aagcgacaaa tatgaaaaaa accctgttgg caattgttgc cgtttccgcc
     2581 ttaagtgcct gccggcaggc ggaagaggga ccgccgcctt taccccggca gattagcgac
     2641 cgttcggtcg gacactattg cagtatgaac ctgaccgaac acaacggccc caaagcccag
     2701 attttcttga acggcaaacc cgatcagccc gtttggttct ccaccatcaa gcagatgttc
     2761 ggctatacca agctgcccga agagcctaaa ggcatccgcg tgatttacgt taccgatatg
     2821 ggcaatgtta ccgattggac gaatcccaat gccgacacgg agtggatgga tgcgaaaaaa
     2881 gccttttacg tcatcgacag cggctttatc ggcggtatgg gtgcggaaga cgcgctgccg
     2941 ttcggcaaca aagagcaggc tgagaaattt gcaaaggata aaggcggtaa ggttgtcggt
     3001 ttcgacgata tgcctgatac ctatattttc aaataatatt gaaaaaaccg gccgaaactt
     3061 taaaaacgag tttcggtcgg tttttctttt cttgttcggt atagtgtcgg caggaaagaa
     3121 ccttcacatc ccgccgtaat tcggcccgct cgcgccttcg gggcaaatcc aagtgatgtt
     3181 ttgcgtcggg tctttgatgt cgcaggtttt gcagtgcacg cagtttgccg cgttgatttg
     3241 caggcgcgga ttgccgtttt cttcaacaat ttcgtacacg ccggccggac aatagcgcgt
     3301 ttcgggcgag gcgtattctt tgtagttcac gtctatcatc gtttgcggat tgttcagcac
     3361 caaatggtcg ggctggtttt cttcgtgcgc gagattggca aggaagacgc tgctcaagcg
     3421 gtcgaaggtc aacacgccgt cgggtttcgg ataatcaatc ggcttacacg cggcggcttt
     3481 tttaagctgc tcgttgtctt tgccgtgatg tttcaaggtc cacggggctt tgcctctgaa
     3541 aatcatctga tcgatgccgg tgtagattga gccgaggtaa acgccccatt tgaatgacgg
     3601 acggacattg cgcgcggcgt aaagctcttg atacagccag ctttgttcaa aacgttgctg
     3661 ataatccgcc gcctctttgc cgctgtcgaa accctccact tcttcaaggt tttccaacaa
     3721 ggggaacacg gcttcggcgg cgagcatggc ggatttcatc gcggtatgaa tgcctttgat
     3781 gcgcggcata ttgaggaaac ccgccgcatc gccgaccaaa atgccgcctt tgaacgagag
     3841 cttcggcaaa ctttgcaaac cgccttcaat cagcgaacgc gcgccgtaag caatgcggcg
     3901 gccgccttca aaggttttgc ggatttcggg atgggttttg aaacgttgga actcttcaaa
     3961 cggcgacaga taaggatttt gatagtccaa accgaccacg aagccgacgg cgactttgtt
     4021 gtcgtcgaaa tggtaaacaa acgcgccgcc gtaggttttg ctgtccagcg gccagcctgc
     4081 gctgtgcacc accaaaccgg gctgatgctg ttcggacggc acttcccaaa cttctttaat
     4141 gcccaagccg taagtttgcg gctggctgtt ttggtcgagt tggaaacgtt cgatgatttg
     4201 tttggaaagc gaaccgcgac aaccttcggc aaacagggtt tgctgcgccc aaagctccat
     4261 gccgggttgg aatgaatcgg tcggctcgcc gtctttgcca atgcccatat tgccggttgc
     4321 aatgcctttg accgaaccgt cttcgtgata cagcacttcg gcggcggcaa agcccggata
     4381 gatttccacg cccatatttt ccgcctgctc cgccaaccag cgcacgactt cgcccaagct
     4441 gacgatgtag ttgccgtgat tgtcgaaatt cggggtaatc ggcaggttga acgctttttt
     4501 ctcggtcagg aacaacactt tgtcctgcgt tactgtgcgt gtcagcggtg cgcctttttc
     4561 tttccagtcg gaaatcaact cattcagcgc aatcggatcg ataactgcgc cagccagcga
     4621 atgcgccccc acctccgaac ctttctccac cacgcaaacg ctgatttcgc gcccgttttg
     4681 ttcggcaagc tgcttgagtt tgatggcggc agacaaaccc gacgggcctg cgccgacaat
     4741 cacgacatcg tattgcatac tgtcgcgggt gatggattct gtcatggcgg ttcctgtgta
     4801 tttattattg aattgcaaat ccgtaattat acaacgggaa catatagtta ccaaatacaa
     4861 caaaggtcgt ctgaaaacca tattttcggt tttcagacga cctttgtcga aatttcaata
     4921 agcacgccac cattttacct gtccgaccgc aaactccgtc tgacgtttcg gactgcgtgt
     4981 gaaaaacgcc ttatccccgc cggcatccct ccctttcggc acaaccgcca aaatcttacc
     5041 tgccaaattt ccctcacggg tttgccaagc atccaaaaac tgcgccctgc tcatcgaaac
     5101 atgacccagc gacgggtcgg caagcaaaac cgtattgccg tctataccgc gcaataccga
     5161 gaaatgatca tccttgcggt atttcagata cacgatgacg gggatttgca actgtgcaag
     5221 ctgctcgaaa gacagggcat agcctttcgc ttcaaaaccc aaatcaggca taatgcgccg
     5281 catatcctca aacgacgcgg gcatctgctc cttatccagt ttttttaaca cgtcctcttc
     5341 cgtcagcttt tgcccgtaaa aattgttcaa aagcgtcgcc accgaagccg
//
LOCUS       DAXHKX010000137         5373 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHKX010000137 DAXHKX010000000
VERSION     DAXHKX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.137"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            225..2444
                     /locus_tag="ACFMWZ_001684"
     CDS             225..2444
                     /locus_tag="ACFMWZ_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC5935613.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGSSVETTPVNRPAVGAAMRLPRRNTAFHREDGTVIPDSKQAEEKLSFKEE
                     DILFLYGSKKDQRQQLKDKIRQPNSTASIITSEKKNARYSYKFVDAGYVYVRGEDEIK
                     WTSDYKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDAKRHRAGKAVGI
                     DNLGYITFYGNDIGATSYAAKDVDEREEHPAEYTVDFDKKILKGELIKNQYVQKRNDR
                     KEPKKPLTIYNITAKLDGNRFTGSAKVNTELKTSHADKEHLFFHTDADQRLEGGFFGD
                     NGEELAGRFISNDKSVFGVFAGKQKTETENAADTKPALSSGKHTKILDSLKISVDEAS
                     DKNPREFAISPMPDFGHPDKLLVEGREIPLVNQEQTIDLADGRKMTVSACCDFLTYVK
                     LGRIKTERPAAKPKAQDEEDSDIDNGEESEDEIGDEEEGTEDAAAGDEGSEEDEATEN
                     EDGEEDEAEEPEEESSAEGNGSSNAILPVPEASKGRDIDLFLKGIRTAETNIPQTGEA
                     RYTGTWEARIGKPIQWDNQADKEAAKAEFTVDFGKKSISGTLTEKNGVKPAFHIENGK
                     IEGNGFHATARTRENGIDLSGQGSTRSQSFKADNLLVTGGFYGPKAEELGGTIFNNDG
                     KSLGITENIENKVEAEVEAEVEVEVEADVGKQLEPDEVKHKFGVVFGAKKDMQEVEK"
     gene            2441..5275
                     /gene="lbpA"
                     /locus_tag="ACFMWZ_001685"
                     /gene_synonym="tbpA"
     CDS             2441..5275
                     /gene="lbpA"
                     /locus_tag="ACFMWZ_001685"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC5935614.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSNKTGQFFLVEGENTLK
                     PVAKLAGYGIYLNNQLNRWVKERIEKKQPLSAEEEAMVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKTVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEHTG
                     EYTADDKDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
BASE COUNT         1550 a         1251 c         1437 g         1135 t
ORIGIN      
        1 aggttcgtgg gaatgacgaa gtagaagtta cccgaaactt aaaacaagcg aaaccgaacg
       61 aactagattc ccactttcgt gggaatgacg ggatgtatct gtactgtctg cggctcgtcg
      121 ccttgtccgg atttttgtta attcactata aaaacgggtt gatattatct gtacatatta
      181 atataatgat aattattatt aatcaaatag gaggaaaagt agggatgtgt aaaccgaatt
      241 atggcggcat tgtcttgttg cccttacttt tggcatcttg catcggcggc aatttcggcg
      301 tgcagcctgt tgtcgaatca acgccgaccg cgtaccccgt cactttcaag tctaaggacg
      361 ttcccacttc gccccctgcc gggtcttcgg tagaaaccac gccggtcaac cggcccgccg
      421 tcggtgcggc aatgcggctg ccaaggcgga atactgcttt tcatcgtgaa gatggcacgg
      481 taattcccga tagcaaacaa gcagaagaaa agctgtcgtt taaagaggaa gatatcctgt
      541 ttttatacgg ttccaaaaaa gatcaacgtc agcagcttaa agataaaatt cgtcaaccaa
      601 attctacggc aagcattatc acatcggaaa agaaaaatgc aagatatagt tataaatttg
      661 tagatgcagg ttatgtatat gtaagggggg aagatgaaat taagtggact tcagattaca
      721 agcagttttc caaccgctta ggttatgacg gttttgtata ttattccgga gaacgtcctt
      781 cccaatcttt accgagtgcg ggaacggtgg aatattctgg taactggcaa tatatgaccg
      841 atgccaaacg tcatcgagca ggtaaggcgg ttggcattga caatttgggt tatatcacat
      901 tttatggtaa cgatattggt gcaacttctt atgcggctaa ggatgtcgac gaaagggaag
      961 agcatcctgc cgaatatacg gttgatttcg acaaaaaaat cctaaagggc gagctgatta
     1021 aaaatcagta tgtgcaaaag agaaatgatc gaaaggaacc taaaaaaccg ctgaccattt
     1081 acaacattac tgcaaaattg gacggcaacc gctttaccgg cagtgccaaa gttaacaccg
     1141 agttgaagac gagccacgct gataaagagc atttgttttt ccataccgat gccgatcagc
     1201 ggcttgaggg cggttttttc ggcgataacg gagaagagct tgccggacgg tttatcagca
     1261 acgacaaaag cgtattcggc gtattcgcag gcaaacaaaa aacagagaca gaaaacgcag
     1321 cagatacaaa acctgccctg tcgtctggaa aacacaccaa aatcttggat tctctgaaaa
     1381 tttccgttga cgaggcaagt gataaaaatc cccgtgagtt tgccatttcc cctatgcccg
     1441 attttggtca tcccgacaaa cttcttgtcg aagggcgtga aattcctttg gtaaaccaag
     1501 aacaaaccat cgatcttgcc gacggcagga aaatgaccgt cagtgcttgt tgcgactttt
     1561 tgacctatgt gaaactcgga cggataaaaa ccgaacgccc cgccgccaaa ccgaaggcgc
     1621 aggacgaaga ggattcggac attgataatg gcgaagaaag cgaagacgaa atcggcgatg
     1681 aagaagaagg caccgaagat gcagccgcag gagatgaagg cagcgaagaa gacgaagcca
     1741 cagaaaacga agacggcgaa gaagacgaag ctgaagaacc tgaagaagaa tcgtcggcag
     1801 aaggcaacgg cagttcaaac gccatcctgc ctgtcccgga agcctctaaa ggcagggaca
     1861 tcgacctttt cctgaaaggt atccgcacgg cagaaacgaa tattccgcaa actggagaag
     1921 cacgctatac cggcacttgg gaagcgcgta tcggcaaacc cattcaatgg gacaatcagg
     1981 cggataaaga agcggcaaaa gcagaattta ccgttgattt cggcaagaaa tcgatttccg
     2041 gaacgctgac ggagaaaaac ggtgtaaaac ctgctttcca tattgaaaac ggcaagattg
     2101 agggcaacgg tttccacgcg acagcgcgca ctcgggagaa cggcatcgac ctttccgggc
     2161 agggttcgac cagatcgcag agcttcaaag ccgacaatct tcttgtaacg ggcggctttt
     2221 acggcccgaa ggcggaggaa ttgggcggta ctattttcaa taatgatggg aaatctcttg
     2281 gtataactga aaatattgaa aataaagttg aagctgaagt tgaagctgaa gttgaagttg
     2341 aagttgaagc tgatgttggc aaacagttag aacctgatga agttaaacac aaattcggcg
     2401 tggtattcgg tgcgaagaaa gatatgcagg aggtggaaaa atgaataaga aacacggttt
     2461 tcagcttacc ctgactgcct tggctgttgc cgccgccttc ccgtcatatg cggcaaaccc
     2521 tgaaacggcg gcacctgatg cggcgcagac ccaatccctg aaagaggtta ccgtccgtgc
     2581 cgccaaagtg ggacggcgat cgaaagaggc gaccggtttg ggcaaaatcg tcaaaacgtc
     2641 ggaaacgttg aacaaagaac aggtactcgg tatccgcgac ctgacgcgct acgatccggg
     2701 cgtggcggtt gtcgaacagg gcaacggcgc gagcggcggc tactcgatac gcggcgtgga
     2761 taaaaaccgt gtggcggttt cggttgacgg cgttgcccaa atacaggcgt ttaccgtgca
     2821 gggatcgttg agcggatacg gcggacgcgg cggcagcggt gcaatcaacg aaatcgaata
     2881 tgaaaacatc agcacggtgg aaatcgataa aggtgccggt tcgtccgatc acggcagcgg
     2941 cgcactcggc ggcgcggtcg ccttccgaac caaagaggcg gcagacctga tttcagacgg
     3001 caaaagctgg gggatacagg caaaaaccgc ctacggcagt aaaaaccgcc aatttatgaa
     3061 gtcgctcggc gcggggttca gtaaagacgg ttgggaaggg ctgctaatcc gaaccgaacg
     3121 tcaagggcgg gaaacgcgcc cgcacggcga tattgcagac ggggtggcat acggcataaa
     3181 ccgtttggat gcgttccgcc agacacacga tattcaaagc aataaaacag ggcaattttt
     3241 cttagtagag ggcgagaata cactcaagcc cgtggcaaaa ttggcgggct acgggatata
     3301 tttgaacaac cagctcaacc gctgggtaaa agaacgtatt gaaaaaaaac agcctttaag
     3361 tgctgaagaa gaagcgatgg tgcgggaggc gcaggcgcgc cacgaaaacc tgtccgccca
     3421 ggcttacacg ggcggcggca ggatattgcc cgatccgatg gattaccgca gcggctcttg
     3481 gcttgccaag ctgggctacc gcttcggcgg caggcattat gtcggcggcg tgtttgagga
     3541 taccaaacag cgttacgata tccgcgatat gacggaaaaa cagtattacg gtacggacga
     3601 ggcggacaag tttagagaca agagcggggt gtacgacggc gacgatttcc gcgacggctt
     3661 gtattttgtg ccgaatatag aagagtggaa gggcgataaa aagttggtca ggggcatagg
     3721 tttgaaatat tcccgcacca aatttattga cgaacatcac cgccgccgcc gtatgggttt
     3781 gctgtaccgt tatgaaaacg aagcgtattc tgacaattgg gcggacaaga cggtgttgtc
     3841 gtttgacaaa cagggcgtgg caaccgacaa caacacgctg aagctgaatt gcgccgtgta
     3901 tcctgctgtg gacaaatcct gccgcgcgtc ggcggacaaa ccgtattcct acgacagcag
     3961 cgaccgtttc cactaccgcg aacagcacaa tgttttgaat gcctcgtttg agaaatcgct
     4021 gaaaaacaaa tggacgaaac accatctgac tttgggcttc ggttacgatg cttccaacgc
     4081 gatttcccgc cctgaacagc tttcccacaa tgcggcaagg atttcggaac acacggggga
     4141 gtacaccgcc gacgacaaag acaagtaccg tttgggtaag cccgaagtcg tcgaagggtc
     4201 ggtctgcggc tacatcgaaa ccctgcgttc ccgcaaatgc gtgccaagaa aaatcaatgg
     4261 cagcaatatc catatttctt tgaacgaccg tttttcaatc ggcaaatatt tcgatttcag
     4321 cttgggcggc aggtatgacc ggaaaaactt caccacgtcg gaagaactcg tccgcagcgg
     4381 acggtatgtt gaccgttcgt ggaacagcgg catcgtgttc aaaccgaacc ggcatttttc
     4441 cgtgtcttac cgcgcctcca gcggcttcag aacgccctca ttccaagaac ttttcgggat
     4501 agacatttat cacgattatc cgaaaggctg gcagcgtccc gccctgaaat cggaaaaggc
     4561 agccaaccgc gaaatcggtt tgcagtggaa gggcgatttc ggctttttgg aaatcagcag
     4621 cttccgcaac cgctataccg atatgattgc cgttgccgat cacaaaacca aattgccgaa
     4681 tcaggcagga caattgacag agattgatat acgcgattat tacaatgccc aaaatatgtc
     4741 gctccaaggc gttaatatat tgggaaaaat cgactggaac ggtgtgtacg gcaaactgcc
     4801 cgaaggtttg tacaccacat tggcgtacaa ccgtatcaaa ccgaaatcgg tatccaaccg
     4861 gccgggactg tccctccgca gctatgcttt ggatgcggta cagccgtcgc gttatgtttt
     4921 ggggttcgga tacgaccagc cagaggggaa atggggcgca aacattatgc tgacctattc
     4981 caaagggaaa aaccctgacg agcttgctta tctggcaggc gatcaaaaac gatattcgac
     5041 aaaaagagcg tcgtcttctt ggtcgacggc agacgtttcc gcctatctga atctgaaaaa
     5101 acggctgacc ttgagggcgg ctatctacaa tatcggcaac taccgctacg ttacttggga
     5161 atccttgcgc cagactgcgg aaagcacggc aaaccggcac ggcggcgaca gcaactatgg
     5221 aaggtatgcc gcaccgggca ggaacttcag cctcgcgctt gaaatgaagt tttaaaggaa
     5281 ataccgtctg aaagcttgat ctgcacccca aaagtcggac taaaccgcca actgattaag
     5341 gtgcaggttt ttttgattcg atataaacaa aat
//
LOCUS       DAXHKX010000138         5321 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHKX010000138 DAXHKX010000000
VERSION     DAXHKX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.138"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            295..1728
                     /locus_tag="ACFMWZ_001686"
                     /pseudo
     CDS             295..1728
                     /locus_tag="ACFMWZ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            1837..2625
                     /locus_tag="ACFMWZ_001687"
     CDS             1837..2625
                     /locus_tag="ACFMWZ_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC5935615.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2993..4549
                     /locus_tag="ACFMWZ_001688"
     CDS             2993..4549
                     /locus_tag="ACFMWZ_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC5935616.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4566..5312
                     /gene="surE"
                     /locus_tag="ACFMWZ_001689"
     CDS             4566..5312
                     /gene="surE"
                     /locus_tag="ACFMWZ_001689"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC5935617.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1240 a         1345 c         1523 g         1213 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttatatat
       61 tattaaaggg ctgcattact tttctttcta aattaccatt gtataaaaag tatttttctt
      121 aaacgacagg gattgcgccc gcgccatatt cagacaccgc ccggatgttg cgctgcccga
      181 tcggatgctt cagacggcat cggaagggtt tgcagttttg gaatatgaag atgataatgt
      241 ccgcgagatt gacggcattt gaaagcggtt attccgcata ccggaggata cgaaatgaac
      301 gaatattccc aattaatcaa gcatcccgat atttcccttt ccccgatttc agacagcatc
      361 gggatcggca atccggcgac gggtgaaatt ttggcgtatg tccgcaacac gggttcggac
      421 aaactgaaaa acctgattta aaaggcggcg gcagcgcaaa aattatgggc ggcgaaaact
      481 gctttggaac gcgccgacat attgtggcgt tggtattttt tagttaaaga aaacaaagaa
      541 gcactggcgc gcctgatgac gatggagcag ggcaaaagcc tgaccgaggc gcgcggcgag
      601 attgattatg cggcttcgtt tgtgcgctgg tttgcggaag aggcgcggcg gattgacggc
      661 gatgtgttaa cgagtgtaaa agcgtcgcaa aaactggttg tgttgaaaca gcctgtcggc
      721 gttaccgccg cgattacgcc gtggaacttc ccatctgcga tgattgcgcg caaggccgcg
      781 cctgctttgg cggtgggctg cgcgatgatc gtcaaacccg catcgctcac gcctttgagt
      841 gcgtatgcgt tggtcgtgct ggcttatgaa gcgggcgtac cacaggattt gttgcccgtc
      901 gtcagcggcc gcgcatcgga aatcggccgt gaatttgcca cgaacccgat tatccgcaaa
      961 atcagcttca ccggctctac cgaagtcggt gcgaaaattt ttgccgacag cgcggcggac
     1021 attaaaaaac tcagtttgga actgggcggc aacgcgccgt ttatcgtgtt tgacgatgcc
     1081 gatttggaca aagccgtcga aggtgcgctc gccagcaagt tccgcaacag cggtcagacc
     1141 tgcgtctgca ccaaccgcgt ttacgcccaa tcgggcattt acgacgcatt ttgccgcaaa
     1201 ttaagtgaaa aagcagccgc gctcaaattg ggcaacggct tggaggatgg cgtaaaccaa
     1261 gggccgctga ttgaggaaaa agcggtggag aaagtcgagc agcacatcgc cgacgcgctc
     1321 gctaaaggcg cggtctgtct gaccggcggc aaacgcagcg cattgggcgg aacgtttttt
     1381 gagccgaccg tcttaagcgg cgtaacggcg caaatggcgg tggctcgcga agaaaccttc
     1441 gggcctttgt gtccggtatt ccgttttgaa accgaagccg aagtcattga ggctgcgaac
     1501 aatacggaat acggtttggc tgcttacctt tttaccgccg acaccgcacg ccagtggcgc
     1561 gtcggcgaag cattggaata cggtatggtc ggcatcaata cgggcttaat cagtaatgaa
     1621 gcggcaccgt tcggcggcgt gaaacgcagc ggtttgggac gtgaaggcag caaatacggt
     1681 gcggacgaat atctggaatt gaaatatctg tgtatggatg tcgggtgacg gatgccgtct
     1741 gaacggcagg gtttcagacg gcgccgcatt ttaagcagtc tctatctgtt gtacaatgcg
     1801 ccctgttttt acggttattt ttgatttgaa caagatatga tggaaaacgg aaaaacagtc
     1861 ccgagatggc gttttgcctt gaaaagtgcg ggctggcatc tcttaatcag cctgtcggtt
     1921 gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta tgccgagctg
     1981 acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg tggtccgctg
     2041 ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt cgatttttcc
     2101 atggtcggca tcatccagct ggcggctttg gcgtacggtc tgcacagcgt ttcgctggcg
     2161 cgtcctgtgg tggaagcgtt tgaacaggat cgtatgacca ttgttacggc ggcggaagtc
     2221 gtggtcgaag atttgcacaa agcccccgaa gggctgcaaa gcctgtcgtg gttcggcatc
     2281 cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac gctggatttg
     2341 tccctgaaag gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta ttccgacaag
     2401 gaagcagaag aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga tgcgagaaaa
     2461 acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga gctgtattac
     2521 ctaccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa agagggcaac
     2581 accaaaggct acgcgccgat agacggcttc atcatcaccc cttaagcgtt gggactccgt
     2641 ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga gaggggcgca
     2701 acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg tgttgaaaca
     2761 tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcgt tttgccattg
     2821 ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc acatgcaaga
     2881 ccccgagcag agcagcaagc ccgcccgccg ttttttgtgc gtaccgtctg ttcaggcagc
     2941 ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa tcatggattt
     3001 cagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg tgttggaagt
     3061 cgtattgggg atagacaatc ttgtctttgt ggcgattttg gcaaacaagg tccagcccgc
     3121 acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc gcatcattat
     3181 gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa tcggcggcct
     3241 cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc tttacaaagc
     3301 caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg ccgacagcca
     3361 aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg atgccgtgtt
     3421 ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg tggcgatggg
     3481 tgcggtcgtc gtcgcgatgg ctgtaatgat ttctgccagc aaactcttga ccgaatttgt
     3541 cgacagacac cctaccgtcg tgatgctctg ccttggtttt ttgttgatga tcggtttcag
     3601 cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg ccgccatcgg
     3661 cttctcgatt ttaatcgaat tgtttaacca gatttcgcag cgcaacagcc gcaaaaacga
     3721 ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gtcttgggta tgatgggtat
     3781 acgcgaaagc gtgcttgccg acgcgggcgg cgaatccggg gacgacgcgc attttgaaga
     3841 aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga ttatgggggt
     3901 gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg aagaacagtg
     3961 tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg cgggcgtgga
     4021 cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg aaacaggcgg
     4081 tctcgacatt cagacggcat tgcgccagcc gctcgtcctg cccgacagca ccaccgcgct
     4141 gggcgcaatc gaactcttcc gccaaagcag cgcggattat gctttggtgg tggacgagtt
     4201 cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg caggcgagtt
     4261 ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg atgaaagcct
     4321 gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc tgccgcagca
     4381 ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat tgcaaaccat
     4441 ccccgatgtc ggcgattttg ccgatttcca cggctggcgg tttgaagtgg tcgaaaaaga
     4501 agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag cattcaggat
     4561 agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggc attgccgttt
     4621 tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt tgcgcccgaa cgcgacagga
     4681 gcggggtcag caattcgctg acgctggaac gccctttgca gttgaaacag gcgcaaaacg
     4741 ggttctacta tgtcaacggc acgccgaccg actgcatcca catcgggcag tctgtatttt
     4801 cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat atgggggacg
     4861 acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg ggcatacccg
     4921 ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcaaccgca gaaaaggcac
     4981 tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt ttgtggaaca
     5041 tcaatatccc cgccgtcgag ccggaagatg tgcggggcat taaaatcgcc cgtttgggca
     5101 ggaggcatca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa cagatttatt
     5161 ggataggccc ggtaggcagg gtttccgaca aagaagaggg aacggatttc ggtgaatgcg
     5221 gcgcaggttt cattaccgta acgccgctgc aaatcgacct gaccgcctat ccggacatgg
     5281 cggaaacagc ggcgttctgg catgcggact gaccgtttca t
//
LOCUS       DAXHKX010000139         5297 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHKX010000139 DAXHKX010000000
VERSION     DAXHKX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.139"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(207..1562)
                     /locus_tag="ACFMWZ_001690"
     CDS             complement(207..1562)
                     /locus_tag="ACFMWZ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC5935618.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(1577..1849)
                     /locus_tag="ACFMWZ_001691"
     CDS             complement(1577..1849)
                     /locus_tag="ACFMWZ_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC5935619.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1974..2711)
                     /locus_tag="ACFMWZ_001692"
     CDS             complement(1974..2711)
                     /locus_tag="ACFMWZ_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC5935620.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2992..3891)
                     /gene="prmB"
                     /locus_tag="ACFMWZ_001693"
     CDS             complement(2992..3891)
                     /gene="prmB"
                     /locus_tag="ACFMWZ_001693"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC5935621.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            4036..4629
                     /locus_tag="ACFMWZ_001694"
     CDS             4036..4629
                     /locus_tag="ACFMWZ_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935622.1"
                     /translation="MVDGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIITKLDDLLRYAKYEGSYSDRKGRPDIIAAH
                     KYRAVTKVGNESLNVGVIVREFPDGHKHYDHFILKDE"
     gene            4741..5297
                     /locus_tag="ACFMWZ_001695"
                     /pseudo
     CDS             4741..5297
                     /locus_tag="ACFMWZ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224992.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT         1475 a         1352 c         1154 g         1316 t
ORIGIN      
        1 cacctgcgac aaacagttca atgagtttat tttgtttata ctggcttaga cgactttttc
       61 tcatagggat aattctaact taatttgaat ttccctagtt atctaggaca gccccatatt
      121 cttttctaaa gacattttac ccgatgccta taaacaaaaa tgccgtctga agcctttcag
      181 acagcatttc cccatcaaaa ccgcaatcag tttttcatcg attggaccgg agcgggaatt
      241 ctgccgcccc gtttgacgaa cacttcgcac gaaccttctt ttaccggcat cacaggtgcg
      301 tagcccaaca ggccgccaaa ctcgacgctg tcgccgacgg ttttaccggt taccggaata
      361 atgcgcacgg cagtggtttt actgttaatc ataccgatgg cggcttcgtc ggcgataatg
      421 ccggaaatgg tgtgcgcggg tgtgtcgccg ggaacggcaa tcatatccaa gccgaccgaa
      481 caaacggcgg tcatcgcttc gagtttatcc aacgtcagca cgcctgcttc ggcggcggca
      541 atcatacctt cgtcttcgga aacggggata aacgcgccac tcaaaccgcc aaccgcgctc
      601 gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc tgctgttgtg
      661 ccgtgcgtac cgcagacgct caaacccatt tcttcaagaa tgcgcgccac cgagtcgccg
      721 acggcagggg tcggtgccag cgacaagtcg agaataccaa acgggatatt cagcattttt
      781 gaggcttcgc ggccgatgag ttcgcccacg cgggtaattt tgaaagcagt tttcttcact
      841 acttccgcaa cttcggtcaa tgtcgttgca tctgaatttt ccaacgcggc ttttacgaca
      901 cctgggccgg atacgccgac attgataacg gcatccgctt cgcccgaacc atgaaacgcg
      961 cccgccataa acgggttgtc ttccaccgca ttgcagaaca cgacgatttt cgcacagccg
     1021 aaaccttcgg gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag cttgaccgca
     1081 tccatattga taccggcacg cgtactgccg acattgatgg agctgcacac gatgtcggtg
     1141 gtcttcatcg cttcgggaat ggaacggatt aacacctcgt ctgaaggcga catgcctttt
     1201 tgcaccagcg cggaaaagcc gccgataaag gacacgccga tggctttggc agccttatcc
     1261 aaagtttgcg ccacgctgac ataggaatcg gcatgggtag ccgccgcgat ttgggcaatc
     1321 ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcaga cagatatttt
     1381 gccgtcgcca ccaagtcttt gccgaccgtg gtaattttgt tgtaaatgtt ttggttcaac
     1441 acattgatat cgctgctgat gcagtcgtgc agatcaatgc cgatggtaat ggtgcggaca
     1501 tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga ttggatactc
     1561 atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttca ttttgcatac
     1621 ggatatcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaacc tcctgccgag
     1681 atttggggca tttcgaggta tccaccaaga taatcatggt aaaaaaatca tccatcagct
     1741 gttggctgat attaaggatg ttgattcggt tttccgccaa aattttggaa acatcgtaca
     1801 cgatgccgac gcggtctttg ccgatgacgg tgatgactga attgttcaca ggcttactcc
     1861 ttgcagatat ccgttaaagt ccgaaattat accaccatcg gattttgagg aaatattgtc
     1921 aacaatatat acatacaaaa tgccgtctga aactatttca gacggcatca aatttagggt
     1981 tcgattaaat aaccatcctt atcccactgg gttttcctaa ccaacttatc atcctgataa
     2041 acagcttcac tctttttaga accatcttca taccactcta agactactcc attacgttta
     2101 tgatgatgga tagacagttc cgagagtaat cgaccgcttt catcccatgt cagaatcttg
     2161 gcaggctcat cattgaccat aaccatttcc gttttgatat tgccgtcggc ataccattgc
     2221 ttccatacgc cgtttgcctt attctgttta aactggattt cgctttcctt gccgccgtta
     2281 cggtaatagc ggtaccccgt accttcactc aaaccatttt tataaggcat aacggcagat
     2341 tttttaccgt tcggatacca gttgacccac tccccgtccg gcttaccctt gctgaagccc
     2401 cccgccattt ttttctgacc attaaaatgc cacaaaatca acataccgtt ttgcagggta
     2461 ggcacaaaag atttgatttg cgttgaagca acgatataag gttcggaata tttcttcatc
     2521 gacggataat aaaaatcctg cgcgtgcgca ataccctccg ccacactata ttgcctgata
     2581 taagcggcag aagacatcgt cgccgtcagt ttcccgttct gattaaaata aacagaatag
     2641 gtctgcgccg gcaaagcggc cgaaaaaccc aacaggacag ttgaaaatac aatccgagat
     2701 aattttttca ttgcaatagc gatataaaaa caaggctgtg ttttagtaat cttttgattt
     2761 caattatttg caagggaaaa gacaattatt ttccggttag gaataaacct attctattga
     2821 atatattgaa gccaagtacg cctatcaaca ctatattaaa acactgccaa aaacaattaa
     2881 cttataaaca atatggtaag gatttctctg ccaagcatca aacccgagac aacgtatcgt
     2941 aaaaatgccg tctgaaaaca aatcgtcttc agacggcatt tccccttcaa ctcactcttc
     3001 acccaataac tgctcgcgcg tcaagaggaa aacaaaaccg tcgcccccgc tggtttccaa
     3061 ccaagtaaaa ggcaactccg gatacgctgc ttccaataca tccctgttat gcccgatttc
     3121 caccagcaat acacctttgg gattcagaaa ctttgccgca ttcagaagaa tctgcctggt
     3181 ggcatccaac ccgtccgccc cgctgcccaa tgccaattcc ggttcgtgca aatactcttc
     3241 aggcaataac tcaaccgatt ccgcatccac ataaggcgga ttggaaacaa tcaaatcata
     3301 agtgccttcc aatccttcaa acaaatccgt atgaataagc cggatgcgtt cttccaaacc
     3361 ataatcttcg acattaatcc ctgccacttc caaagcatcc aagctcacat caaccgcatc
     3421 aatttgggca tcaggataat gatgcgccat ctgaatggca aggcaaccgc ttccggtgca
     3481 aagatccaaa gcattatgca ccagctcatc gtattctatc caaggacgaa gtccgttacc
     3541 caacaattca taaataaaag aacgaggtat gattacgcgc tcatccacat agaaatcaaa
     3601 ctctccctgc catgcctggt gtgtcaaata agcggctgga atgtgttcga cagcacgacg
     3661 ctcaataacc gccagcactt cctctttttc agcttccaag agttttgcat caagatatgg
     3721 ggcaagcata tccaaaggca aattcaaagt atgcagaatc aaataagccg cttcatcatg
     3781 cgcattatct gttccatgac caaaaaagag ccctgcctca ttaaaacggc tgactgcaaa
     3841 acgtaaaata tcgcggatag tcgtcaattc ttgtgctgcc tgattaaaca taatatgaac
     3901 cattctgcgt atagatactt ttaattataa cagaaacaac aagcaaacct tttcatatcg
     3961 ccaaataacc acccaatcta cccatacaac tacataaatg cccgcgcgaa aaccatcgcc
     4021 cgaacggaaa cgacaatggt cgacggcatg ggcaatctga ttggctggga aaaaacgggg
     4081 cttgttgtcg gtaagcagtg gataaccgca aaagacgaca aggtgtccga tgtctgcaat
     4141 gccaacggcg agatgggcgt aatcgggctt tacgagcctt tctcacacgg cgcattgacg
     4201 atacccggtc atccgaactg ccgatgcgag gttgtttccg tatcgggtgg cgaattggga
     4261 gaatttgccg aaaaaaagga gcttcgtaaa gcggctatgc agtatgcgcg ggataacttt
     4321 atcggcaaaa gctatgtcaa taaaaacagc gggcatgaac tgaaggtaac ttggcaaggt
     4381 gtgaaacacg ctgcgtcaaa ggcaaatcag gcggaattat ccatcataac aaaacttgat
     4441 gacttattgc gctacgcaaa atatgagggt tcttattcgg ataggaaagg tcgtcctgat
     4501 attattgcag cacataagta tcgtgccgtt accaaggttg ggaatgagtc tttaaatgtc
     4561 ggcgtgattg taagggagtt tccagacggc cataaacatt acgaccattt catcttgaag
     4621 gatgagtaaa gcccttttgc agtgtagctc tggagcggat accgttaagg caagtacgct
     4681 tccagcctta caaaagggct ttaaattcag cataccattt atacaaacag aaataaacca
     4741 atgacgaagt tatacgcaga aatcgccaag atggaggcca ggacgacggc acggtcaaag
     4801 tttggggcta tgcctcaagc gaggcggtcg attccgacgg cgaagtcatc gcggcggcag
     4861 ctatgaaggc ggcgattccc gattatatga agtttggcgc ggagcgcgag atgcacggct
     4921 caaacgctgc gggaacggca attgaaatca acgtggaaga tgacggcaga acctttttcg
     4981 tggcgcatat cgtcgatccc gttgccgtga cgaaggtcaa aacaggcgtt tacaagggct
     5041 tttccatcgg cggcagcgtt accgcccacg atgagttgaa caagtcgcaa atcacgggtt
     5101 tgaagctgac ggaaatcagc ttggttgacc gacccgccaa tcccgatgcg gtgtttacct
     5161 gctttaaggc ggacaaaggt gcggaagcgg taaacaacga tacagaacat aatgctacat
     5221 attttagcca tttcccttcc aaacaaaaaa caaaaaagca ccggcggcgg ccgatgcccc
     5281 ttcctttaca ggttccc
//
LOCUS       DAXHKX010000140         5279 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHKX010000140 DAXHKX010000000
VERSION     DAXHKX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.140"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="ACFMWZ_001696"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="ACFMWZ_001696"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC5935623.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="ACFMWZ_001697"
     CDS             785..1771
                     /locus_tag="ACFMWZ_001697"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC5935624.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="ACFMWZ_001698"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="ACFMWZ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC5935625.1"
                     /translation="MLSKISESLVNLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="ACFMWZ_001699"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="ACFMWZ_001699"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC5935626.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4579..5001
                     /locus_tag="ACFMWZ_001700"
     CDS             4579..5001
                     /locus_tag="ACFMWZ_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC5935627.1"
                     /translation="MALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEWEE
                     RYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSEL
                     ACKTAKTEARLEELHNRKKALIDEMAREADKKELSKRL"
BASE COUNT         1339 a         1469 c         1395 g         1076 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc ggccgacata ttgctcaacc
       61 cgtttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cgcagtttca gacggcaatc gacggttcgg caggtgtcgc gttggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcgggcgagg gcgggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 cggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcgcgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggcgcg cgcggcggcg tgtacctgtg tggcggcatt attccccgcg tgttggaata
     1621 tttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagtttccc ggaatttccg gtgcggctgc
     1741 ggctctcgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggta aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gtccatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaacgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt gttgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgtcgcgtc gagttttacg
     2221 gtgtcggcaa ttccggcatt gtggcacagg acgcgcagca taaatttttc cgtttcggca
     2281 tgtccaccgt cgcctatgtc gatacgcaca cgcagctgat ggcggcatct gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtctg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga cattctcgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg catcccacaa atgccgtctg
     2701 aaatgccgaa caacggtcgt cggcggcttg cggcagtttc cagcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacactt tcacgactta cccgcatggt
     2821 cgaaattgtg gaaccacttt gacgacagca aaacattgca tatgcgcgaa atgttcgagc
     2881 aagacccgca gcgtgcggaa cgctactggc tgcaagtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagcttgcc cgagaagcag
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcgaaaa aatcaatacc accgaaaacc
     3061 gcgccgtcct gcatgtcgcc cttcgcaacc gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gcccaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagcgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggcccgctga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccgcgcct caatatgcac ttcgtctcca acgtggatgg ctcccaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgctgt tcatcatcgc ctccaaaaca tttaccacgc
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgacg
     3481 aagaagccgt tgccaaacac ttcgccgccg tttccaccaa tcaaaaagcc gtcgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggattg ggtcggcggt cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcattgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac acttcatcaa cacaccgctc gagcgcaacc
     3721 tgcccgtcat tctcgccctc atcggcatct ggtatatcaa ctactacggc ggcggcagcc
     3781 acgtcatcgc gccttacgac caacatttgc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag taacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ctggggcgaa acgggcatta acggccagca cgccttcttc caactgctgc
     3961 accaaggcac gcacattacc cccatcgacc tgattgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggcgc
     4141 gcatcgaaga gctggtcccg cacaaaacct tctccggtaa ccgcccgacc aacctcatcc
     4201 tgatggacaa ggtcaacccg cgcaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg ccgcaaaagc
     4381 acgacagttc gaccgaacgc ctgatcaacc tctacctgca gaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcaaacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catatatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagt gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagccgccc gcgtatccga atgggaagaa cgctacaagc tgtcgcgcag cgagttcgag
     4741 cagttctgga aaggattgcc tcaaaccgta cagaataagc tgcaagcctc acagaaaaca
     4801 tggaaaagcg ggatggataa aatctgtgcc aacaatgcga aagctgaagg taaaacgcca
     4861 aacggcataa aattcagcga actggcatgc aaaacggcga aaaccgaagc acgcttggaa
     4921 gagctgcaca accgtaaaaa agcccttatc gacgaaatgg ccagggaagc ggacaagaaa
     4981 gaactgtcaa agcggctctg aacagcgcgg ttcaggcact gcccgccgat attgccgaaa
     5041 ccgtcatgcc cgagtaccgc aactgacaaa acggtttgaa tgccaaatgc gccgacggcg
     5101 acgaatacgg cgtggcacaa tctgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgtt tttatagtga attaaattta aaccgataca gcgttggctc gccttagct
//
LOCUS       DAXHKX010000141         5189 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHKX010000141 DAXHKX010000000
VERSION     DAXHKX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.141"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            188..1372
                     /gene="ubiM"
                     /locus_tag="ACFMWZ_001701"
     CDS             188..1372
                     /gene="ubiM"
                     /locus_tag="ACFMWZ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC5935628.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1629..1784)
                     /gene="rpmG"
                     /locus_tag="ACFMWZ_001702"
     CDS             complement(1629..1784)
                     /gene="rpmG"
                     /locus_tag="ACFMWZ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC5935629.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1816..2049)
                     /gene="rpmB"
                     /locus_tag="ACFMWZ_001703"
     CDS             complement(1816..2049)
                     /gene="rpmB"
                     /locus_tag="ACFMWZ_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC5935630.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2299..3825)
                     /gene="farB"
                     /locus_tag="ACFMWZ_001704"
     CDS             complement(2299..3825)
                     /gene="farB"
                     /locus_tag="ACFMWZ_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC5935631.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSSGGGGH
                     "
     gene            complement(3849..5003)
                     /gene="farA"
                     /locus_tag="ACFMWZ_001705"
     CDS             complement(3849..5003)
                     /gene="farA"
                     /locus_tag="ACFMWZ_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC5935632.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1327 a         1456 c         1243 g         1163 t
ORIGIN      
        1 ataccccaca cccgatttga tacacattaa gctgaaagta aaaatccgca tacaccctcc
       61 cctgcatatt tcttcaacag tgggttttga tataatcgcc tatctgttac agatagttca
      121 aacggcattc aaacccttac aaataccgtc tgaaatcatc ggcatccgtc tatgcaaagg
      181 atattttatg agtttacaca gcgacatcct cgtcgtcggc gcaggcccgg cgggtttaag
      241 ttttgcagcc gaacttgccg gcagcggttt gaaagttacc ctgatcgaac gcagcccact
      301 gactgttctg caaaatcctc catatgacgg acgcgaaatc gccctgaccc atttttcccg
      361 cgaaatcatg cagcgtttgg gtatgtggga caaaattccc gaaaacgaaa tctacccttt
      421 gcgcgatgcc aaagtgctga acggacgttc cgactaccag ctccactttc cccaaccgac
      481 cgaggcgcgc ggcgagcctg ccgactgttt gggctatctg atttccaacc acaatatccg
      541 ccgcgctgcc tacgaagtcg tatcccaact cgacaacatt accattctga ccgatacggc
      601 cgttaaagaa gtcaaaacat ctgacaacga agcacaagtc attttagaaa acggcaaaat
      661 cctgaccgcc cgcctgcttt tggcggcaga cagccgtttt tcgcaaaccc gccgacaact
      721 cggcatttct tcagatatgc acgactacag ccgaaccatg ttcgtctgcc gcatgaaaca
      781 tactctttcc aaccaacata ccgcttacga atgcttccat tacgggcgca ccatcgcact
      841 gctgccgctg gaagaacacc tgaccaatac cgtcattacc gtcgataccg ataaaatcaa
      901 cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc gtaaaagaac agctcaaggg
      961 caggctcggc gatatggaat tggtcagcag catccaccat tacccccttg tcggtatgat
     1021 tgccaaacgt ttctacggca aacgcagcgc gctgatcggc gatgcggctg tcggtatgca
     1081 ccccgttacc gcacacggtt tcaacttggg tctgtccagc gcagatattt tggcaaaact
     1141 gatactcgaa gccgagcagc gcggtcagga catcggcgcg gcttccttgt tggaaaaata
     1201 cagcagcaaa catatgctgc acgcccatcc gctttatcac gggaccaata tgatgctgaa
     1261 gctgtttacc aacgaaaccg caccggcaaa actgctgcgc ggcttggtat tgcgtgccgg
     1321 caacaatttc ccgccgctga aaaagctgat taccaaacaa ctgaccggtt gaaaactgcc
     1381 gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt atagtggatt aacaaaaatc
     1441 aggacaaggc gacgaagccg cagacagtac aaatacactt ggtgcttcag caccttagag
     1501 aatcgttctc tatccttggc agacggtttg cagcagattc gcatacggca gatgtttcaa
     1561 gcggaaaaga aacacgataa aaataaaaaa gcctccgaac agtcggaggc tttgctttca
     1621 aactgaaatt atttcagttt ggtttctttg tacactacgt gtttgcgggc aaccggatcg
     1681 aattttttaa tttccagttt gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag
     1741 tgaccagtac ctgcactgga ttccagtttg attttatcgc gcattgcagt aatccttaat
     1801 taaatagtgt ttaaattaag cttcgccgcg agcacgcaaa tcagccaata cgacatcaat
     1861 gcctactttg tcgatggtac gcagagcggc gttagaaacg cgcaggcgaa cccagcggtt
     1921 ttcactttct acccaaaaac gacgtgattg caagttgggc aaaaaacggc gtttggtttt
     1981 gttgttggcg tgcgatacgt tgttgccgga catcgggcgt ttgccggtca ctttgcaaac
     2041 tcgtgccata atcagtctcc aaacttctaa aatagtaaaa cgcgatttat accacgaaaa
     2101 acagtgttag ttcaagtgtt ttaaagaaaa acggctgttt tgcgcggata atccttccga
     2161 ttgtcgcatc tgtggaacgt ttgcaggtgc gaaacagatg gggcgcgatt atagcggatt
     2221 tcgggaactg ctgcattcaa aatgccgtcc gaacatattt ccagtatttt cagacggcat
     2281 ttcaagtttt caaatccctc aatgtccacc gccgccactg ctgtggaacg gcggttttgc
     2341 cagccatatg acgggtatca tgataatgaa taacaagctg cctgccataa agatttcatt
     2401 cgatccgatg atgaagccct gctgggtaat ggtattgttg atgataccta gggtttgaat
     2461 gtcggaaatg ccttgttggg acaaatgcgc cgccgtttcg tgcaatgttg cggaataggg
     2521 cgtgatgtgt tcggcaaagc gcgtgtggtg caacgcttcg cgccgttccc acagggtgct
     2581 gacgacggat acgccgacac cgcccatcag cacgcgcagg aaattcgaca ggctgccggc
     2641 ggcggcaatc tgcccgccct tcatgtgcga cagtgtgatg gtggtcagcg gcaggaagaa
     2701 catggcaacg cctgccccct gccaaaactg cggccagatg acgttgccga tatccatgtc
     2761 ggcataaaaa tcggtgcgcc aatagaaagt aaaggcaaag gtcaggaagc tggcggttac
     2821 gagcaggcgc atatcgattt tattgccgaa cctgccgatt aacggagaca ggaagacggg
     2881 caggatgccg acgggtgcgg cggcaagccc cgcccacgtg gaggtatagc ccaggttggt
     2941 ctgcaacact aacggcagca aggtcagcgt ccccatatac accataaaac ccaatgatgt
     3001 ggcaattacg ccgacggtaa aattccgatc tttaaacagc gataaatcga caatcggata
     3061 tttttctccc aattcccaaa caataaaata cgacaaacac accagtgcga ctacgcccaa
     3121 ggtaatgatt tctccagagg cgaaccagtc gagttcctta cccctgtcca gcatcatctg
     3181 taacgcgccg ataccgacta ccatcaatgt aagcccgaca tagtcggtcg gcattttaac
     3241 ggtttccgtt tcccgatatt tcaaatgttt ccatgtaatc catgccgata tgataccgat
     3301 agggatatta atgaagaaaa tccaacccca atgccagttt ccggaaatcc aaccgccgag
     3361 tatcggcccg agaacagggg cgacaacgac ggtcattgcc cacaatgcca gtgccagcgt
     3421 ccgttttgcg ggcggatagg atgccattaa caggctttgc gacaggggaa tcagcggccc
     3481 ggcgataaag ccctgcaaga tgcggaaaat taccaaagcc tgaaggttgg gcgcgatgcc
     3541 gcacagccac gatgtgatga cgaaaccgac agcggcggcg gtaaacaatt tgacctcgcc
     3601 gatacgtttt gccaaaaagc ccgttaacgg cacggaaacg gcgtttgcca cagcaaaaga
     3661 agtaatgacc cacgtcccct gcgtggttgc cgcaccgagg ttgccggcga tgacgggaac
     3721 ggcgacattg gcgatagtcg tatctaaaac ttccataaat acggcaagcc ccaaagacag
     3781 cgtaacccac gccaatgccg cacccttaag cggtggataa tccattgtgt cctccgtttc
     3841 agacggcatc agggcgcgga ttgcccgagg atttcatcga ccgtccgatc gacttccgac
     3901 cagtcggtac tttccatttc cggcaatgcc gcacccggcg tttttgaaac aggcgcgcct
     3961 gcggcggaag tatccacttt aaccgtcatc gacaaaccga tacgcaacgg atgcctgtcc
     4021 acatcttcgc ggttcagcac gatacggacg gggacgcgct gcaccacttt aatccagttg
     4081 cccgttgcgt tttgcgccgg aatcagcgaa aacgcgctgc ccgtacctgc cgaaaaacct
     4141 gccaccctgc cgcgataaac aatttgtttg ccgtacaaat cggacaccag ctcggcaggc
     4201 tgtccgattt tcatatgccg caactgcgtc tctttaaaat tagcatccac ccacacatcc
     4261 gacagcggca ccaccgccat cagcggcgcg cctgccgcca cctgctgccc gacctgcacc
     4321 gaacgcttcg ccacctgacc gtccgccggc gcgcggattt gcgtccgctg aaggttcaac
     4381 cacgcatctt tcaacctgcc gattgccgtc tgaaccgccg gctgttcgcg caaagaaacc
     4441 tgaccgccca aagccgcacg tgccgaagac tcttccgcca aagccgcctt aactgccgcc
     4501 tgagcctgag acactgccgc acgggcatgt gccagctctt cggcggacac tgcgcccgat
     4561 tccgctaaag cagaccggcg gcgtaaatca tcctgcgcac gtgccaaatc cgcccggcgc
     4621 aaggcaacct gcgcccccgc ctgcgaagtg gcggcatttt gccggcggtt ttgccgcacc
     4681 gcctgaacca gctcgttttt tgcccgctcg taagccagca catcattatc gtcgtccaat
     4741 accgccagca cgtcgccttt tttcacggca tccgtatcgt cgtgcaaaac cttccgcacc
     4801 gtaccgccct tttgcggcgt aacctgaacc acgcgtccgg caacataagc gtcttccgtt
     4861 tcctcttcgt gctgccacca taaaaaaaac gccgacccgg cggctgcggc ggcaagcgcg
     4921 aacagcagcg tcaatgccgt caggcggcgt ttgcgtttgg cttgcgtgtt ttgaagtttt
     4981 gtttcgtccg tgtgcgtatc cataagattg ggttccgaat ttcctgattt catataaaca
     5041 atgttctttc aataggaagc agattctata tccgcgcaat aacgcaatca agtcagtatt
     5101 ttagatgcaa tattcaaaaa acaacgcttc aggcggcaaa actgaaaata tatttcgttt
     5161 atataatgga ttaacaaaaa tcaggacaa
//
LOCUS       DAXHKX010000142         5098 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHKX010000142 DAXHKX010000000
VERSION     DAXHKX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.142"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(7..1263)
                     /locus_tag="ACFMWZ_001706"
     CDS             complement(7..1263)
                     /locus_tag="ACFMWZ_001706"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC5935633.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLMYATCSVLPEENELQVERFLSEHSEFELVNCAELLQSLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(1611..1952)
                     /locus_tag="ACFMWZ_001707"
     CDS             complement(1611..1952)
                     /locus_tag="ACFMWZ_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935634.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2156..3013)
                     /locus_tag="ACFMWZ_001708"
     CDS             complement(2156..3013)
                     /locus_tag="ACFMWZ_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC5935635.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVARLNGKPYHIELTCKYYGGGTGRPEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQSFHAFEPPLNPYGWRGIYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3074..3889)
                     /locus_tag="ACFMWZ_001709"
     CDS             complement(3074..3889)
                     /locus_tag="ACFMWZ_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC5935636.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTTNRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3953..4123
                     /locus_tag="ACFMWZ_001710"
     CDS             3953..4123
                     /locus_tag="ACFMWZ_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935637.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            4133..4918
                     /locus_tag="ACFMWZ_001711"
     CDS             4133..4918
                     /locus_tag="ACFMWZ_001711"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC5935638.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLVILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            complement(4899..>5098)
                     /locus_tag="ACFMWZ_001712"
     CDS             complement(4899..>5098)
                     /locus_tag="ACFMWZ_001712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935639.1"
                     /translation="IVQGSPFNQIWVSIFGSFGHLAVSTFLPPLPVKMPSEISARYFR
                     RHTRPFSRIPGSGLKFKRVRK"
BASE COUNT         1148 a         1192 c         1389 g         1369 t
ORIGIN      
        1 accggtttat ttgcgttgca atacggcggc gaagaagccg tcggtttggt gtcgggcgga
       61 atcgaggcgc aggtattttc cggtatccaa atcaaccttc aggctttgca gcagttcggc
      121 gcagttgacg agttcaaatt cggaatgttc ggacaggaaa cgttcgactt gcagctcgtt
      181 ttcttccggc aacacgctgc aagtggcgta catcaagcgg ccttgcggtt ttaccagttt
      241 ggaggcggca tcgaggatgc tgtgttgctg ttccaaaagg ttggccacgg tttcggcgga
      301 ttggcggtat ttgagatcgg gattacggcg taaagtaccc aaaccggagc agggcgcatc
      361 cactaaaacg cggtcggctt tgcctgccag ccgggcgata cggctgtcgt gttcgctgct
      421 gatgcgttca gggtggatgt tggtcagtcc ggcgcgggtc atacgcggtt tgaggttggc
      481 aaggcgtttt tcggcgatat cgaaggcgta gattctgcct ttgttcgcca tttgcgcacc
      541 gacagccaag gttttaccgc cggcaccggc acagaaatcg acaatgattt cgccgcgttt
      601 tgcgcccacc aataaggcaa gcagctggct gccttcgtct tggacttcca gtgtgccgtc
      661 taaaaacagt tcgtgtttgt taagcgcgat tttgtttttc aggcggatgc cccaaggcga
      721 ataaggcgtt gcctctgcat cggcactttc ggcttgcaac aatggcagca ctttatcgcg
      781 tttgcctttc aaggtattga cgcgtatatc gagcggcgca gcttggttga tgctgcggcc
      841 gaaggcgagg atttcttctt cgcgccaatg ctgctgcagc tgttctacca gccattgcgg
      901 caattctgcg gcggtattca ggccgtctga aaactcggtt ttacgggctt tcaaattgcc
      961 gagaaactct atttcttctt catcaagcag gtctttgatt tggctgatgt tggtgcttct
     1021 gccgagaacc agtgcggcga gtgcggcttt gcgcggctgc gcgtgcggac ggcgcagggc
     1081 ggtactgatt ttttgatagt ggcgcagcgc ggcaaaggcg gtttcggcga tttcgtggcg
     1141 gtcttggcgg ccgagctttt tgtgttcgcg gaaataggcg gagaggacgg catcggaagg
     1201 ctgtttgaaa gtcagcattt cagccaaaac tttggcggta tggtcgagtt gtgcggcgtt
     1261 cataatgtaa tgggttctct tactgtgggt tattcataat caatctaacg tccttaaaat
     1321 aacaatagca tctttatcat tacttagtat tctatccgca ataagaaaga tgtgttaaaa
     1381 gtgcgcaatc gggatttgtc taaatttctc cgttaactac aaaggcagcc tgaaaaaagt
     1441 tctattttca ggtcgcttga acagcatccg atataatcac atcagcgatt tcattttgta
     1501 atcactccct aaaacatgag gaaaaaaaca aaatttattt tcttcgttca gtatatctat
     1561 cctaaatatg ccatcactcc attgagtttt taatttgtat ttcgctcttg ttaaatgttt
     1621 ttctgtttgc cgatttcttt tgctaatgta aaaaatactc taccactctc attggttaaa
     1681 gttttcaagt attcatcaac atcagtaaca ttaataacaa ctgtttcatt tgtatgttta
     1741 ttaaattcta aatcaataat aatattccca ttaaccaata tttcatatat atcttcttct
     1801 tcagggagca tagacaaaca ataagcactt gaagctgaaa actctttttt cagatactga
     1861 agaagaaaag tatttttaat taaaatatta ttaaatttat ctaatgattg cttgacttct
     1921 ttagttgtcc aatttttaac attattcttc attgtattct acctggggtc gtagttatta
     1981 aattaccata cttatttgtt tggatcgtca taatattagt tggcatgttg ttgttatatt
     2041 tatcatatcc aatatcctta ttaactcgga tgactctttc ataaattgta tgggaaatga
     2101 gatttccatc actaagcctc tcaatagata tacgcgttaa tgttgggaaa gggtttcaga
     2161 cggccttcat aatgcgttcg atttcgtgcc aaaagccgtc cggccgacat tccaaaacgg
     2221 caatcaagcc ttggtcgata cggcggattt cggtttcgtt caatgtttcg gtttcggcgg
     2281 cacgcgcagg tgcgaggtag gccatgcggt cgagcagatg gtagcggact tcttggcgtt
     2341 caaacccgta ttccgcccag tcttgaatat agatgccgcg ccagccgtat gggttaagcg
     2401 gcggctcaaa agcatggaaa ctttgtggaa aaaatccgat gccgcgcacg atggaaacgg
     2461 gttttacgtc aagcggcaga ccgtgttgcc gcaaggttcg gatgccgtct gaagtgtgcg
     2521 gcagaccgag ttgggcggtc agtttggcgg cttttcccaa cagcgtgtct ttggggtcga
     2581 gtccgcgcat gtcttcgggt ctgcccgtgc cgccgccgta atatttgcag gtcagctcga
     2641 tatggtaggg tttgccgtta agccttgcca caaaatccgc cgcgccttgc gtattgccgt
     2701 ccgaaccgga aaccgtgagg ttgtgcgcga gcagttcggc gtgtggcgcg ttggcaaacc
     2761 aaaatgccag cagttcttca gcgtaaatgc ccaagcggtg tccgaacggg gcgcgtcggg
     2821 tgaggtgttc cgtcagcggc gcgggatcgg catccaatgc caaaagatag cggaaaccgc
     2881 gttcccccaa tagttcgcgc acgcccaatt cgcaaccgct ttgccacaaa ggcggcgcag
     2941 tcagcagcga ggcgaggtcg cggacgggtt ggctggtgag cttccaccat aatgcgtcta
     3001 gggcgtaatt catgcttatc cgaatatcag tcatgccgtc tgaaaagctg gaaatgtttt
     3061 cagacggcct taatcagtct tttttatgga tacgatggct gacggtattg aaaaaaccgc
     3121 gcaggatatc gatgtcttcg gtttgcgtgt tggcgcgtcc gaacaggctc tgcatacggc
     3181 gcatcagacg ctcgccgttg cggcggttga aaaagccgat gtcgttcatc acgctttcca
     3241 tgtgggcgac catgcctttg atttgctcgt gggtcgcagc gtggtcttct tgttgaagat
     3301 gggtcatggg cgaaccggtt tggctgaaga tttcgtagca cacgacctgc acggcttggg
     3361 cgaggttgag cgagaaatag tcgggattgc cgttgatggt catcagtcgg ttgcaggctt
     3421 ggacttcttc gatgctcaag ccgaaagtct cgttgccgaa aaccagtgcc actttctcgc
     3481 ctcggtttgt ggtctgcagt aattcggata ccaaatcgcg cggggtttgc agcggcgcag
     3541 taatttcgcg gcggcggctg gtcagggcgc aggcgatggt ggtgtcggca agggcttcgt
     3601 ccaaagaagc ggcaatggtg gcattttcca aaacgtctgc cgcgccggaa gcgaggatga
     3661 agctttcttc cggtaattta aacgattgag gatgctccgg gtcaaacacg ggcgggtttt
     3721 ccgtcatcgg cgttgccatc agatttgggg cgacgatggt cagtttgtgc agacccattg
     3781 ttttcatcgc gcgcgcggca gagccgatgt tggcgggatg gctggtgcgc gtgaggatga
     3841 tgcggatgtt gtccagataa gcgggcaggg cgggtttgag agtagtcatg ttcggttttc
     3901 ggtcggaatg gaatagggta taatgcgccg tctttttcgt tcggacggtc aaatgccgtc
     3961 tgaaatgttc tttaacaacg tcggaaattt aacgttgcgc ctgaagcaga cacgcgtcgg
     4021 gcggtatttt acccgatttg gcaggattta ccccaaccaa ataaactccg attttgccgc
     4081 tccgaaggac tgcgcgtccg aaccggcggt atgccaccct taaggaaatc tgatgaatcc
     4141 gtttttgaat acagccttta aagccgcccg ccgtgccggt cagatgatga tacgcgccgc
     4201 gggcaatctc gatgccgtca aaaccgacag caaagcattc aacgattttg tttccgatgt
     4261 tgaccgcaat tccgaaatca tcctggttga ggctttgaaa gaagcctatc cgcaccacaa
     4321 aatcacttgc gaagaaagcg gctcccacgg taaagccgca gccgaatacg aatggattat
     4381 cgatccgctc gacggcacga ccaacttcct tcacggccat ccccaatacg ccatctctat
     4441 ggcactcctg cacaaaggcg tgttgcaaga agctttggtt tacgcccccg aacgcaacga
     4501 cgtatatatg gcttcgcgcg gcaaaggcgc gttgctcaac gaccgccgca tccgcgtttc
     4561 caaccgcatc gaattgaacc gctgcctgat cggtaccggc ttccctgttg tcgatcaaag
     4621 catgatggac aagtatctgg tgattttgaa agatttcttg gccaaaaccg ccggcggccg
     4681 tcgtgaaggc gcggcttctt tggatttgtg tgctgtagca acaggtcgtt ttgacggctt
     4741 tttcgagttt aacctcaaac cgtgggacat tgccgcaggt gcattgattg ttcaggaagc
     4801 gggcggtatc gtaaccgata tgtcgggtga agacggctgg ctggaaagcg gcgatattgt
     4861 ggctgccaat cccaaagtgc tggcgcaaat gttgaaaatc atttccgcac acgtttaaat
     4921 ttcagcccgc tcccgggaat gcgggaaaac gggcgggtat gccgtctgaa gtatcgcgcc
     4981 gatatttcag acggcatttt tactggcagc gggggcagga aggtggaaac ggctaaatgc
     5041 ccgaaactgc cgaagatgga aacccatatc tgattaaacg gagaaccctg tacgattt
//
LOCUS       DAXHKX010000143         4985 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHKX010000143 DAXHKX010000000
VERSION     DAXHKX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.143"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(41..1033)
                     /locus_tag="ACFMWZ_001713"
     CDS             complement(41..1033)
                     /locus_tag="ACFMWZ_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC5935640.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            1527..4286
                     /locus_tag="ACFMWZ_001714"
     CDS             1527..4286
                     /locus_tag="ACFMWZ_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC5935641.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVHAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPKPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRHSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQDRNVQPAAPVADAAPSETEVQTGKRRRNGSRSE
                     RSQTAPETAAVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         1270 a         1589 c         1251 g          875 t
ORIGIN      
        1 cgtcccgatg ccgtctgaag gcttcagacg gcatcggggt tcacattttc gtcccgtttt
       61 ccaacatcac cgcaaactgc gccaagtccg gcggcagctc cgccttcaac accagcggct
      121 cgcccgtgag cggatggttc aagtgcagct cggacgcgtg caaaaacatc cgcttcaaac
      181 ccaacttctg caaacgacgg ttcgcctgat aatcgccgta gcgttcgtcg cccgcaatcg
      241 gacagccttg agattgcagg tggacgcgga tttggtgcgt acgacccgtt ttcagcgttg
      301 cccgcaccaa agtcaggtgc gacagcccga caccgtgcaa aatgccgtct gaaaaacggc
      361 ttaacacacg gaacaccgtg tgcgccgact gcccgtcctc gctgacgcgt accatctttt
      421 cgccttgtgc gccggtatat ttgaacaggg gcagtttgac atggaaattg tcgtccggca
      481 gtttgcccac ccccagcgca aggtagattt ttttggggtg gtcgttacgg atggcttcgt
      541 gaagtttgac gagcgcgctg cgtttcttcg ccaccatcaa caagccgctc gtatccttgt
      601 ccaaacggtg gacgagttcg agataacgcg cctccggacg ggcgcggcgc aactgttcga
      661 taacgccgaa actcacgccg ctgccgccgt ggacggcaac gccggacggt ttgtcgatga
      721 ccaaaagcgc atcgtcttcg taaacaacgt caaacgcacg cgccggtacg gcggcacgcc
      781 tttcagacgg catttccttc tccgccacgc gcacaggcgg aatccgcacc gtatccccct
      841 ccgcaatacg gctgtcgggt ttgcagcgtt tcttgttcaa ccgcacctcg ccggcgcgga
      901 taatgcggtg gatatggctc ttgggaacac ccttgaggat ttttatcaga tagttatcaa
      961 ggcgttgacc cgcctcatgt tcggcaaccc ctatcaagct gaccgaatct ttgcttattt
     1021 cgtgcgtttt catctataat ccgaacatcc gtttcagcaa aaagcgcgcc cgccgcgccc
     1081 ccctgaaacg gtttacttta aaacggaatt ttatattaaa aacgcactcc gcaaagcatt
     1141 ttccctgcgc ctgttcccag ccggcaggcg cagaacgtaa tcaagtttga attgattttg
     1201 ccgtttcggc gcggaagtaa gacggcagcc gggccccaag tcccaaacgc aaaccgccca
     1261 aaacacaggc atcacgataa caagaagaag ccggccccca tttttccgac cgcagcgttc
     1321 ggaacgcggc agacaaaccg ccgcatcgat ctttatagcc ttctgtatcc gacccttccg
     1381 cacacggttc tccggaacgt gcatccctgc ggattttcaa ctcaaaaact gattaccggt
     1441 tttatccgaa aacatcggaa aacccagcct tacggaatgc cgaaccggac cggcgcgccc
     1501 gaatgccgcc cgtccacgag gtgatcatga aaagaatgtt attcaacgca acgcaggccg
     1561 aagagctgcg cgttgccatc gtcgacggac aaaacctgct ggatttggac atcgaaacgc
     1621 tgggcaaaga acagcgcaaa ggcaatatct acaaaggcat cattacccgc atcgagccgt
     1681 cgctggaagc gtgtttcgtg gattacggaa ccgaccgcca cggtttcttg ccgtttaaag
     1741 aagtctcccg ctcatacttc caagactacg aaggcggacg cgcgcgcatc caagacgtgc
     1801 tcaaagaagg catggaagtc atcgtccaag tcgaaaaaga cgagcgcggc aacaaaggcg
     1861 cggcgctgac caccttcatc agcctcgccg gacgctatct ggtattgatg ccgaacaacc
     1921 cgcgcggcgg cggcgtatcc cgccgtatcg aaggcgaaga acgtcaagaa ctcaaagccg
     1981 ctatggcgca actcgacatt ccgaacggca tgagcatcat cgcccgtacc gccggcatcg
     2041 gccgcagcgc ggaagagttg gaatgggatt tgaactatct caaacaactc tggcaggcga
     2101 ttgaagaagc aggaaaagcg catcacgacc cttacctgct ctttatggaa agctcgctgc
     2161 tcatccgcgc catccgcgac tatttccgcc ccgacatcgg cgaaattctg gtggacaatc
     2221 aagaagttta cgaccaagtt gccgagttca tgagctacgt catgccgggc aatataggcc
     2281 gtctgaaact ctacgaagac cacacgccgc tgttttcccg cttccaaatc gaacaccaaa
     2341 tcgaaagcgc gttttctcgc agcgtcagcc tgccttccgg cggcgcaatc gtcatcgacc
     2401 acaccgaagc cctcgtctcc atcgatgtca actccgcacg cgccactcgc ggcgcagaca
     2461 ttgaagacac cgcgttcaaa accaatatgg aagccgccga agaagtcgcg cgccaaatgc
     2521 gcctacgaga cttgggcggt ttggtcgtca tcgacttcat cgacatggaa aaccccaaac
     2581 accagcgcga tgtggaaaac gtcctgcgcg acgcgctcaa aaaagaccgc gcccgcgtgc
     2641 agatgggcaa actctcccgt ttcggacttt tagaattgag ccgccaacgt ttgaaaccgg
     2701 ctctgggcga aagcagccac gtcgcctgtc cgcgctgcgc cggcaccggc gtgattcggg
     2761 gcatcgaatc caccgccctg cacgttttac gcatcattca agaagaagcg atgaaggaca
     2821 acaccggcga agtacacgcc caagtgcccg tcgatgtcgc caccttcctg ctgaacgaaa
     2881 aacgcgccga gctgtttgcg atggaagagc gtttggatgt gaacgtcgtc ctgattccga
     2941 acatccacct cgaaaatccg cactacgaaa tcaaccgcat ccgcaccgac gacgtagaag
     3001 aagacggcga accgagctac aagcgcgttg ccgagccaga agaagacgaa tccgccaaac
     3061 cgttcggcgg cgaaaaagcc aaagccgccc gtcccaaacc cgccgtcaaa ggcgtgcgcc
     3121 acaccagccc cgccccgact gccgcccccg agaaaaaaac ctcctggtgg gacagcttca
     3181 aagcatggct caaacgcatt ttcggcggca gcgaaaccca agccgcgccc gctgccgaaa
     3241 cctccgaaaa acgcagcacg gcaaaccgca gcggcagccg cgccaacaac cgccgccaaa
     3301 acccgcgcca cagcaaacgc gaaggcagca aagtagaagt ccgcgaagtg gcaggcaaaa
     3361 ctgccggaca ggaagcgcgt gccgacaaag ccgaaacgcg caacaacggc aaccgccgcc
     3421 gcaacgaacg cggcgaccgt gccgccgaac gcgccaacga agcggaaatc caagaccgca
     3481 acgtacagcc tgccgcaccc gttgcagatg ccgcaccgtc cgaaaccgaa gtgcaaaccg
     3541 gaaaacgccg ccgcaacggc agccgcagcg aacgcagcca aaccgcgccg gaaaccgccg
     3601 ccgttgccga aacaaccgtt cagacagcgg aaaacacgcc gtccgaaccg cataccgcag
     3661 aagacaaagg cagcaagccc aaatccgaac gcaaccgccg cgaacgcgac agccgagatg
     3721 ccaaagaacg ccgcgagcgc aacaatcagc gcgaccgccg tcaaaacggc aaaaaacgca
     3781 atattccgtc tgccgccaaa atcgagcagt acctgaatat tcacgacacc gccgacaaag
     3841 tccgttccgc cgccgcgcac gttttcggcg aaaccgacgc aaacgcgccg attaccgtca
     3901 gcattgccga tccggttgca gaaagggatc ttccgacagc atctcccgcc gtttcaaacg
     3961 gcgacgcacc ggtttatgat gcggcggaaa aaatccgccg tgccaccgcc gccatcctgc
     4021 ccgaaggcgc gacaccgaaa gccgaagcac aggaaatgcc gtctgaaacc gcaaccttta
     4081 cggctgcggc ggaacaggca cgggaaaccg cacaaaccgg cggacttgtc ctgatcgaaa
     4141 ccgaccctgc cgcattgaag gcatgggcgg cacaacccga agtccaaacc ggacgcggtt
     4201 tgcgccgttc cgaacagccc aagccgtctg aagtcgcaac cgtccctgcc gaagaaatga
     4261 tccaagtcga aacccggcaa ggctgaaccg acggcggcaa aaagaggttc tgttccgcag
     4321 aacctctttt ttatatagat tcggatacct gcaatgccgt ctgaagcctc accattccca
     4381 tgattaccgc aacctttcgt cattcccgtg aaaacgggaa tctagaacct caaactttca
     4441 aataaccttt gaatattgcc gctgccttaa ggtctggatt cccacctgcg cggaatgacg
     4501 gtttagaagt tgcccgaaac ctcaaaaaaa actgaaaccg aacaagccgg attcccgcaa
     4561 cattccgtca ttcccgtgaa aacgggaata tagaacccca aactttcaga taatctttga
     4621 atattgccgt cgcccaaagg cctggattcc cgcctgcgcg ggaatgacgg cggagggtgg
     4681 acgatgccgt ctgaaacttc gccattccca tgattaccgc aacctttcgt cattcccgcc
     4741 acctttcgtc attcccgtga aaacgggaat atagaacctc aaactttcag ataatctttg
     4801 aatattgccg ccgcccaaag gtctggattc ccgcctgcgc gggaatgacg gcggagggtg
     4861 aacgatgccg tctgaaactt caccattccc atgattaccg caacctttcg tcattcccgt
     4921 gaaaacggga atctagaacc tctaaacttt cagataatct ttgaatattg ccgtcgccca
     4981 aaggc
//
LOCUS       DAXHKX010000144         4897 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHKX010000144 DAXHKX010000000
VERSION     DAXHKX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.144"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            242..4843
                     /gene="iga"
                     /locus_tag="ACFMWZ_001715"
     CDS             242..4843
                     /gene="iga"
                     /locus_tag="ACFMWZ_001715"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC5935642.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGANN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTSHASTITLTGKSLITDPKTISIHYIQNNDDDDDGYYYYRPRKP
                     IPQGKDLYFKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     NITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALNSFDATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSSVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLDLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     EKVAHQKAEEAKRQQDALARQQAEQERQRLEAERQAAEIAKQKAEAEEAKRQAAELAR
                     QQEEARKAAELAAKQKAETERKAAEIAEQKAEAEREAAELAKQKAEEEGRQAAQSQPK
                     RRNRRAIPPELSSDATTRALPRIARNSNPDASDYEEIPLDALEDEDVSESVDTSDKQP
                     QDNTELHEKVETVSLQPRAAQPRAQAAAQPQAQAAAQADAVSTNTNSALSDAMASTQS
                     ILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDR
                     SLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRS
                     RLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVS
                     SMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQQY
                     SAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1657 a         1142 c         1049 g         1049 t
ORIGIN      
        1 aaagcgggaa tccaaaaaca aaaagccgca gaaatttaac ggaagaaccg aaccccgaaa
       61 atcggcttcc cgccttcgct tgaataacag aacaaaggaa gcggaaataa aaacatcggg
      121 attctttttt taaattggca ataaaacacc aaaattaatg attcacacaa caaaaaatag
      181 tcaacaccgc caaaccggcg taaaatacaa aacattatca cccttaaaac ggtaaaacct
      241 tatgaaaacc aaacgtttta aaattaacgc catatcctta tccatctttc ttgcctatgc
      301 ccttacgcca tactcagaag cggcattggt cagagacgat gtcgattatc aaatattccg
      361 tgactttgca gaaaacaaag gcaaattttt tgtcggcgca accgatttat cagtgaaaaa
      421 caaacaaggt caaaacatcg gcaacgcatt atccaacgta ccgatgattg attttagcgt
      481 tgcagatgtc aacagacgta cattaaccgt catcgatccc cagtatgccg tcagcgtcaa
      541 acatgtaaaa ggagacgaaa tctcttatta cgggcatcac aatgggcact tagatgttag
      601 caatgatgaa aacgaatatc gttcagttgc acaaaatgac tacgaaccaa ataaaaattg
      661 gcatcacggt aatcaaggtc gtcttgaaga ctacaacatg gcacgcctta ataaattcgt
      721 aacagaagtc gcacctattg caccaaccag tgcaggcggc ggcgttgaaa cctataaaga
      781 taaaaaccgt ttttctgagt ttgtgcgagt tggagcaggt acgcaatttg aatataacag
      841 ccgctacaat atgacagagc tttcacgagc ttatcgttat gccattgcag gtacgcctta
      901 tcaagacgtg aatgtcacat cgaatctaaa tcaagaaggc ttgattggct tcggtgataa
      961 ttcaaaacat cattccccag aaaaactcaa agaagtttta tctcaaaacg cattgactaa
     1021 ctatgctgtg ttaggcgata gcggttcacc attatttgct tatgataaac aagaaaaacg
     1081 ctgggtcttt ttaggtgctt acgattattg ggcaggctat caaaaaaact cttggcaaga
     1141 atggaatatc tataaaaaag aatttgcaga taaaatcaaa caacgcgata acgccggcac
     1201 catcaaaggt tatggcgaac atcattggaa aaccacgggc acaaacagcc atatcggttc
     1261 gacagcggta aggcttgcca acaatgaaag ggatgcaaac aacggacaga atgttacctt
     1321 tgaagacaac ggcactttgg tattggatca aaacatcaac caaggcgcgg gcggtctgtt
     1381 tttcaaaggc gattacacag tcaaaggtgc aaataatgac atcacttggc taggtgcggg
     1441 gattgatgtt gccgacggca aaaaagtcgt ttggcaagtc aaaaatccga atggcgacag
     1501 attggcaaaa atcggcaaag gcacattaga aataaacggc acaggcgtta accaagggca
     1561 attaaaagtc ggcgacggta cggttattct gaatcaacaa gccgatgccg acaaaaaagt
     1621 ccaggctttc tcccaagtcg gcattgtgag cggacgcggt acattggtat taaatagttc
     1681 aaatcagatt aatcccgata acttatattt cggtttccgt ggcggtcgtt tggatgccaa
     1741 tggcaatgac ttgacttttg aacacatccg caacgtggat gaaggcgcgc gcattgtcaa
     1801 ccacaacaca agccacgcct ccacaatcac gctaacgggt aaatctttga ttaccgatcc
     1861 caaaaccatc tctattcatt atattcaaaa taatgatgat gacgatgatg gttattacta
     1921 ttaccgccct aggaaaccga ttccacaagg caaagatctt tatttcaaaa actaccgtta
     1981 ttacgcccta aaatccggcg gcagcgtgaa cgcaccgatg cccgaaaacg gacaaacgga
     2041 aaataacgac tggattttaa tgggcagcac acaagaagaa gccaagaaaa acgcgatgaa
     2101 ccacaaaaac aatcagcgta ttagcggttt tagcggtttc tttggcgaag aaaacggaaa
     2161 aggacataac ggtgcattaa accttaattt caacggcaaa agcgcgcaaa accgtttctt
     2221 gttaacaggc ggcactaatt taaacggaaa aataagcgta actcaaggca atgtcttgtt
     2281 atcaggtcgt ccaacaccac acgcaagaga ttttgtgaac aaatcttcag cccgaaaaga
     2341 cgcacatttc tccaaaaaca atgaagtcgt atttgaagac gactggataa accgcacatt
     2401 caaagccaca gaaattgcgg ttaatcaatc cgcatcattc tcttccggta gaaatgtatc
     2461 caacatcacc gccaacatca ccgcaacaga caacgccaaa gtaaatttgg gttacaaaaa
     2521 cggcgatgaa gtttgcgtgc gctcggacta taccggttac gttacctgca acacagacaa
     2581 cttatccgat aaggctttaa acagctttga tgcgacacag attaacggca atgtgaattt
     2641 gaatcagaat gcggcattgg tcttaggcaa ggctgcatta tgggggcaaa ttcaagggca
     2701 aggaaacagc agcgtcagcc taaatcaaca tagcaaatgg catttgaccg gcgacagcca
     2761 agtacacaat ctgtcattgg cggatagcca tattcatttg aacaacgctt ccgatgcgca
     2821 aagtgcaaat aaataccaca cgctcaaaat caatcattta tccggtaacg ggcattttca
     2881 ttatctgacg cacctggcga aaaatcttgg ggataaagtg cttgtgaagg aatccgcatc
     2941 cggtcattat cagctccatg ttcaagataa aacaggcgaa cccaatcagg aagggctgga
     3001 tctctttgat gcatcatccg tacaagaccg ctcccgcctt tctgtttcct tggcgaataa
     3061 ccatgtagat ttaggcgcat tgcgttatac aatcaaaaca gaaaacggta ttacccgatt
     3121 gtacaatcct tatgccgaga accgccgccg agtcaaaccg gccccgtctc ctgccacaaa
     3181 cacggcttct caagcacaaa cggacagtgc acaaattgcc aagcctcaaa atatcgtcgt
     3241 cgcaccgcct agcccgcagg caaatcaagc cgaagaagca aaacgccaac aagcaaaagc
     3301 ggagcaagtg aagcgtcagc aagcagaagc agaaaaagtc gcacatcaaa aggctgaaga
     3361 agcgaaacgt cagcaagatg cccttgcccg ccaacaagca gagcaagaac gccaacgatt
     3421 ggaagccgaa cgccaagctg ccgaaattgc caaacaaaaa gcagaagccg aagaagcaaa
     3481 acgccaagct gccgaacttg cacgccaaca agaagaagca cgaaaagctg cagagttggc
     3541 tgctaagcaa aaagcggaaa ctgaacgcaa agcagcagaa attgccgagc aaaaagcgga
     3601 agccgaacgt gaagccgccg aacttgccaa gcaaaaggcg gaagaagaag gtcgccaagc
     3661 agcacaatct caacccaaac gtcgtaaccg cagggctata ccaccggaac tgtcatctga
     3721 tgcaacaacc cgagctttgc ctaggatagc gagaaattca aatcctgatg ccagcgatta
     3781 tgaagaaatc cccttggatg cgttggaaga tgaagatgta tccgaatcgg ttgacacatc
     3841 agacaaacag cctcaagaca atacggaact tcatgaaaaa gttgagacag tgagtttgca
     3901 accaagagcc gctcagccgc gagcccaagc cgccgcgcag ccgcaagccc aagccgccgc
     3961 gcaagccgat gcagtcagca ccaataccaa ctcggcttta tctgacgcaa tggcaagcac
     4021 gcaatctatc ttgttggata caggtgcttc attaacacgg cacattgcac aaaaatcacg
     4081 cgctgatgcc gaaaaaaaca gcgtttggat gtcgaacatc ggttatggcc gtgattatgc
     4141 ttccgcacaa tatcgccggt ttagttcgaa acgcacgcaa acacaaatcg gcattgaccg
     4201 cagcttgtcc gaaaatatgc agataggcgg cgtattgact tactctgaca gtcagcatac
     4261 ttttgatcag gcgagcggca aaaatacttt tgtgcaagcc aacctttatg gtaagtatta
     4321 tttaaatgat gcttggtatg tggccggcga tattggtgca ggcagcttga gaagccggtt
     4381 acaaacgcag caaaaagcaa actttaaccg aacaagcatc caaaccggcc ttactttggg
     4441 caatacgctg aaaatcaatc aattcgagat tgtccccagt gcgggaatcc gttacagccg
     4501 cctgtcatct gcagattaca agttgggtaa cgacagtgtt aaagtaagtt ctatgtcagt
     4561 gaaaacactg acggccggac tggattttgc ttatcggttt aaagtcggca accttaccgt
     4621 aaaacccttg ttatctgcag cttactttgc caattatggc aaaggcggcg tgaatgtggg
     4681 cggtaattcc ttcgcctata aagcagataa tcaacagcaa tattcagcag gtgccgcgtt
     4741 actgtaccgt aatgttacat taaacgtaaa tggcagtatt acaaaaggca aacaattgga
     4801 aaaacaaaaa tccggacaaa ttaaaataca gattcgtttc taaaatacta aattcatagc
     4861 aaaataaaat gccgtctgaa ctcaggcttc agacggc
//
LOCUS       DAXHKX010000145         4871 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHKX010000145 DAXHKX010000000
VERSION     DAXHKX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.145"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            325..1638
                     /locus_tag="ACFMWZ_001716"
                     /pseudo
     CDS             325..1638
                     /locus_tag="ACFMWZ_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519747.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            1882..2538
                     /gene="cmk"
                     /locus_tag="ACFMWZ_001717"
     CDS             1882..2538
                     /gene="cmk"
                     /locus_tag="ACFMWZ_001717"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC5935643.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            2694..4379
                     /gene="rpsA"
                     /locus_tag="ACFMWZ_001718"
     CDS             2694..4379
                     /gene="rpsA"
                     /locus_tag="ACFMWZ_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC5935644.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            4390..4704
                     /locus_tag="ACFMWZ_001719"
     CDS             4390..4704
                     /locus_tag="ACFMWZ_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC5935645.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
BASE COUNT         1280 a         1262 c         1169 g         1160 t
ORIGIN      
        1 ttcaaattcc ctctctttca tcattattta aatgctatca ttaatataat taacaaaatt
       61 tcatttccat acaccgatgt agaagaataa caaattaatc tatttataaa ctccgattct
      121 tcctcatcgg gttatactca tcagaaatac tcactgaaat atatgccttt tacatggaaa
      181 atcactagac agttgaaaag caatcagtca gttcctgtta aaaaacgcgt gtagaatttc
      241 cgctttatct caatcggaca cggtttcaat atgtcttccc cttcaaatac caatcgtcaa
      301 acctgatcca gccgattaac ctatatcctg accgttgccg gcgcgactgt cggtttcggc
      361 gcgacgtggc gtttcccgta tttggtcggt gaaaacggtg gtgcgcgtat gtgtttttat
      421 tctgtatcgc gatgctggtt atcggcatcc cgatgatttt ggtggaaaat gtcatcggac
      481 ggcgcaaagg cgtgaacgcg ctggatgcgt tcggcggccc gatgaacggc aaacccattg
      541 ccaaaatttg gaaactggtc ggctggatgg gctgctcggc gcgttcggca tcatggctta
      601 ttacatggta ctcggcggct gggtaatcag ctatatcgtt aatattattg gaggaaattt
      661 gaatatttcc agccccgtcg acggtgtggt tacaaaaggc ttctttgccg aacacattga
      721 aacagccctt gggaaattgc gttttatacg ctgctttttg tcgccgtgaa ccaatggatt
      781 ttggtcaaag gcgttatcgg cggcattgaa aaagcggcaa aatagctgat gccgctgctg
      841 tttttgttcc taatcgcgat ggtcgtccgc aacgttaccc ttccgggcgc aatggaaggg
      901 gttgctttct atctgaaacc tgatttcagc aagattaccg ccgaactgtt cgtcttcgtt
      961 ttggggcagg tattttttgc cctgagcctg ggtttcggcg tgatgattac cttgtccagc
     1021 tatttggata aaaacgaaaa tctggttcag acggcagtta tcacggcaat taccaatacc
     1081 atcatcgcca tacttgcggg ctttatgatt ttcccgtcgc tcttcagctt cggcgttgcc
     1141 cccgattccg gcccgacttt ggtgttccaa agcttgccga ttgtgttctc acatatgtgg
     1201 gcgggatctg tgttcgccgt gattttcttc tcgctgctcc tgattgccgc attgacgact
     1261 tcgctgacca tttatgaagt gttgattacg accattcagg aaaaaaccaa aatccgccgt
     1321 accgccgcga ttacgattgt attggctgcc atcttcattt tcggcaacat cccgtccatt
     1381 ttgagctacg gcccttggaa agatgtttcc atatttggca aaaatatttt tgacgctttt
     1441 gactacatca gcggcaacat cttgtttatg ctgaccgcgc tcggttccgc gctgtttgtc
     1501 ggttttgtga tgaaggacga agcgaaggaa gaattgcttt ataaaggcaa ccatacgacg
     1561 gtcaatattt ggtttgccta tgtgaaatac ctcgtgccgt tggtgattct gctgattttt
     1621 gtcagcaacc tgttctaatc tgcagcaatc gatgccgtct gaaggccata cctctttcag
     1681 acggcattgc cttgatgccc gccacaatac ccgcaacgcc ggaatccgaa ccggcttgcc
     1741 ggcttctgtc ggatgttttc gggcaggcgg cgttccgtct gcttagacaa tcgtccttta
     1801 aaacaggtag aatccgcccc aacgggaaac acatccttca gacggcaaaa cccatacccc
     1861 aaaccatcag gaatccccct tatgaacaga caaaaagtca tcgccatcga cggcccgggc
     1921 gcatcgggca aaggcacggt tgccgcccgc gttgccgccg cattgggata cgattatctc
     1981 gataccggcg cactctaccg cctgactgcc ctatatgcac aaaaacaagg cgtgggatgg
     2041 cacgatgaag aaaacgtttc cgaactggca aaaaaactgc ccgccgtatt ttcaggcagc
     2101 cgcatcctgc tcggcggcga agacgtttca gacggcatcc ggacagaagc catcggcatg
     2161 ggcgcatccg cagtcgcaca gttgcctaaa gttcgcgccg ccctgctgca acgccaacgc
     2221 gattttctga ccgaaaaagg actggttgcc gacggacggg acaccggatc ggtcgtcttc
     2281 ccccaagccg aacttaaaat cttcctgacg gcagaatcca aaatccgtgc cgaacgccgc
     2341 gccaaacaaa tcggcatccc ctgcgaaggt ttggcattcg agcgcatcct gtccgacatc
     2401 gaagccagag acgaggcaga ccgaaaccgc aaagttgccc ccctgaaaca acagcccgat
     2461 gccctgcttt tggacacaag ccgcctgact atagaagaaa ctgtaaaaaa agtgcttgat
     2521 tggtatcgtg aagtttaaat tttcaggtat aatcgcacaa attacgtttc agacggcata
     2581 aaaatccccc atatgccgtc tgaaacctta tgtacccgtc tgccctgcca aggacggcag
     2641 atatccacca acccaccccg caccccttgg cggtgtaccg aaaagagtta tatatgtcta
     2701 tggaaaattt tgctcagctg ttggaagaaa gctttaccct gcaagaaatg aacccgggtg
     2761 aggtgattac cgctgaagta gtggcaatcg accaaaactt cgttaccgta aacgcaggtc
     2821 tgaaatcaga atccctgatt gatgtagctg aattcaaaaa cgctcaaggc gaaattgaag
     2881 ttaaagtcgg cgacttcgtt accgttacca tcgaatccgt cgaaaacggc ttcggcgaaa
     2941 ccaaactgtc ccgcgaaaaa gccaaacgtg cagccgattg gattgccctg gaagaagcca
     3001 tggaaaacgg cgacatcctg tccggcatca tcaacggaaa agtcaaaggc ggcctgaccg
     3061 ttatgattag cagcatccgc gcattcctgc cgggttcttt ggtcgacgta cgtcctgtaa
     3121 aagacacttc tcacttcgaa ggcaaagaga tcgaattcaa agtgatcaaa ctggacaaaa
     3181 aacgcaacaa cgtcgttgtt tcccgccgcg ccgttctgga agccactttg ggtgaagaac
     3241 gcaaagccct gctggaaaac ctgcaagaag gctccgtcat caaaggcatc gttaaaaaca
     3301 ttaccgatta cggtgcattc gttgacttgg gcggcatcga cggtctgttg cacatcaccg
     3361 atttggcatg gcggcgcgtg aaacacccga gcgaagtctt ggaagtcggt caggaagttg
     3421 aagccaaagt attgaaattc gaccaagaaa aacaacgcgt ttccttgggt atgaaacaac
     3481 tgggcgaaga tccttggagc ggtctgaccc gccgttatcc tcaaggcacc cgcctgttcg
     3541 gcaaagtatc caacctgacc gactacggcg cattcgtcga aatcgaacaa ggcatcgaag
     3601 gtttggtaca cgtctccgaa atggactgga ccaacaaaaa cgtacacccg agcaaagtcg
     3661 tacaactggg cgacgaagtc gaagtcatga ttttggaaat cgacgaaggt cgccgccgta
     3721 tctctttggg tatgaaacaa tgccaagcca atccttggga agaatttgcc gccaaccaca
     3781 acaaaggcga caaaatctcc ggcgcggtta aatccattac cgatttcggc gtattcgtcg
     3841 gcctgcccgg cggcatcgac ggtttggttc acctgtccga cctgtcctgg accgaatccg
     3901 gcgaagaagc cgtacgcaaa tacaaaaaag gcgaagaagt cgaagccgtc gtattggcaa
     3961 tcgacgtgga aaaagaacgc atctccttgg gtatcaaaca actggaaggc gatccgttcg
     4021 gcaacttcat cagcgtgaac gacaaaggtt ctttggttaa aggttccgtg aaatctgtcg
     4081 atgcgaaagg cgccgttatc gccctgtctg acgaagtaga aggctacctg cctgcttccg
     4141 aatttgcagc cgaccgcgtt gaagatttga ccaccaaact gaaagaaggc gacgaagttg
     4201 aagccgtcat cgttaccgtt gaccgcaaaa accgcagcat caaactttcc gttaaagcca
     4261 aagatgccaa agaaagccgc gaagcactga actccgtcaa tgccgccgcc aatgcgaatg
     4321 ccggcaccac cagcttgggc gacctgctga aagccaaact ctccggcgaa caagaataag
     4381 gttgcagaca tgacaaagtc tgagttaatg gttcgtttgg cagaagtgtt tgccgccaaa
     4441 aacggcacgc atcttctggc aaaagacgta gagtacagcg taaaagtctt ggttgacacc
     4501 atgactagat cgcttgcccg aggtcaacgc atcgaaatcc gcggtttcgg cagcttcgat
     4561 ttgaaccatc gtcctgcccg catcggtcgc aatcccaaaa ccggcgagcg tgtggaagta
     4621 cctgaaaaac atgtacccca cttcaagccc ggtaaagaat tgcgcgagcg ggtcgacttg
     4681 gctttaaaag aaaatgccaa ttaaacctta gcatcaaaac gccgctgtta cgcggcgttt
     4741 tttctgtggt ttaacttcat ccgttgcttc aataccttga gccaagcaag caacggatta
     4801 gagcgtggat ttttttatag tggattaaca aaaaccagta cggcgttgcc tcgccttagc
     4861 tcaaagagaa c
//
LOCUS       DAXHKX010000146         4869 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHKX010000146 DAXHKX010000000
VERSION     DAXHKX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.146"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..180
                     /locus_tag="ACFMWZ_001720"
     CDS             <1..180
                     /locus_tag="ACFMWZ_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC5935646.1"
                     /translation="YLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCL
                     NLLKAANRLSAPAAA"
     gene            495..866
                     /locus_tag="ACFMWZ_001721"
     CDS             495..866
                     /locus_tag="ACFMWZ_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935647.1"
                     /translation="MNITLGRNHQINCDKKDLYKFIGYLANHPNDVNLVFEKNSVQGA
                     WGDEGRIQFFSSKAQNIFVPLGFKFTAGVGNIAYRLNCNELFEMLSQLGFVSGGKQNL
                     STIKANIPSQFHAEFDAGANM"
     gene            complement(930..2090)
                     /locus_tag="ACFMWZ_001722"
     CDS             complement(930..2090)
                     /locus_tag="ACFMWZ_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC5935648.1"
                     /translation="MNMENYFSNLNLDIRTHKLGTFTDQKVTPDVLCAVAECIIEYIE
                     NIGEIFSINDIRYSDYAEYIATAVFKKPSIENAGSEYNKFFSQPIKMLSYCGVLSEEK
                     FSRYYRYGVQNNKILQYIANRERNALNFIQAFSEKLLKDSGIYPKFADFFAQPNKNTF
                     ESMKTAFTDLVIQNTPKNTEVEVRRIFTKIINPLAYKHNTFGTRKGSISNTPITLDEL
                     YYNRLNWRDKGKEKSLTRKEAQALFADSANAANLNYLVNKAKKFVKMLHKTSEVQRFD
                     PTEANQAHHIFMASEFPDLASLPENLICLTPNQHFNLAHPSNKTTVIDKHYQRICLMA
                     KLDSIEQDNRANTGNYDYHEFIHVLNTGFNTDQFDVSMSYETLKHRILMFYF"
     gene            complement(2102..4690)
                     /gene="dcm"
                     /locus_tag="ACFMWZ_001723"
     CDS             complement(2102..4690)
                     /gene="dcm"
                     /locus_tag="ACFMWZ_001723"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC5935649.1"
                     /translation="MKNQYSKSLHGVFYLAFYDKIHAFFAEQLFQLIINMNKKPTYIS
                     LFSSAGVGCYGFKTNGFECVATNELLEKRLKIQKYNQKCRFDSGYIAGDITLPETQAK
                     LFAEIRRWNTEIDVVIATPPCQGMSVANHKKNNEMARNSLVVESIKIVREVQPKFFIF
                     ENVKAFLNTPCTDTDHQEKPIEEAIWSNLGGHYNILSNVLNFKDYGANSSRTRTLVIG
                     VRKDIHHITPYDIFPKQTPPKTLRTLLADLPPLMQMGEISDDDIYHSYREFDRRMLPW
                     IAQLKEGESAFQNTDPARIPHQIKNGEIIYNQNKNGDKYARWFLDREGPCIHTRNDIL
                     ASQNTIHPTENRVFSVRELMRMMSIPPSFKWTAQDEKQLNALSFDEKKTFLKKEELNI
                     RHCIGESVPTAVLANVAQNIQAALQQSELSMTEIQNLIKRENLSLADNLYRFIEQHFD
                     TYPFSRLLQIAEYANAARSETAAYFTRIDIAFGLVNALPDFKKKKNLRILEPSVGIGN
                     FIPLLAKKYADKEKVIFDLVDIDSNSLNLLSLLLKKLNLGSKFEFNIIHQDFLTATLP
                     ENYDLVIGNPPYGKVKASDKNLALYRKNATNKDTQNIFSFFIDKALTLSKTVALVVPK
                     SVINAPEFQITRQQLITANINRIIDYGEKAFKGVKIETIAFIADQTDKDNQHITIESH
                     IQETLIQNCKNYFFSDNFPYWLIYRNSEFDQIAQKLEFNVFQSFRDRQITKAHTQNSG
                     NVRVLKSRNIGNNEIIDIEGYDSYVDSVAPFSVGKFINRKNVVMIPNLTYKPRAAFLP
                     ENSIADGSVALLTMKKHEKILTQRALAYYATPEFEQFYRVARNYGSRSMNIDNHSVFF
                     FGLLKD"
BASE COUNT         1434 a          842 c          991 g         1602 t
ORIGIN      
        1 tatttgtcga agacccgtta tgtggtcgaa caaagctttg gtacgctgca ccgtaaattc
       61 cgctatgccc gggcagccta tttcggactg attaaagtga gtgcgcaaag ccatctgaag
      121 gcgatgtgtt tgaacctgtt gaaagccgcc aacaggctaa gtgcgcccgc tgccgcctaa
      181 aaggtgcccc ggatgcctga tgatcgggta tccggggagg attaaggggg gatttgggta
      241 aaatcaggag taattagggg cggaaataga cgaaaacctg tgtttgggtt tcggctgtca
      301 gcgaaaaggt gaagagaggt actttgcaaa ggtctcagcc tgaatatgtc aaccaaatca
      361 gcgacaagct cggagaattt tttggccgga cgttgaaata ggaatttcaa ttcttttaat
      421 tcagcaatag atactgataa aatgaaaatc ttgaggaata tatcattaaa attaatttgt
      481 taaggagata attaatgaat attacattag gtcgaaatca tcaaatcaat tgtgataaaa
      541 aggaccttta caagtttatc ggctatttgg caaatcatcc taatgatgta aatttagtct
      601 ttgagaaaaa tagtgtgcaa ggtgcttggg gtgatgaggg acgtatacag tttttctcaa
      661 gtaaagcaca aaatattttt gtgccactag gatttaaatt tacggcaggc gtaggtaata
      721 ttgcatatag gctaaattgt aatgagcttt ttgaaatgct atctcagtta ggttttgttt
      781 ctggtggaaa acaaaatctt tcaacaataa aagcaaatat tccgtctcaa tttcatgcag
      841 aatttgatgc tggtgcgaat atgtaaattc taatttaaaa cacgaatccg cttcatgaga
      901 ttcgtgtttt ttatttttaa aatatttatt taaaaataaa acattaaaat acgatgcttc
      961 aatgtttcgt aactcataga aacatcaaac tgatctgtat taaatcctgt attgagtaca
     1021 tgaataaatt catggtaatc gtaattgcct gtattagcac ggttatcttg ctcaatacta
     1081 tccagtttcg ccatcaggca aatacgctgg taatgtttgt caataacggt ggttttattg
     1141 cttgggtgtg ccaaattgaa atgctggttt ggtgtcaggc aaatcagatt ttcaggcagc
     1201 gatgctaaat caggaaattc gcttgccata aaaatatgat gtgcctgatt ggcttctgtg
     1261 ggatcaaaac gttgaacttc gctggttttg tgtagcattt tgacaaattt tttggcttta
     1321 ttaactaaat aattcaaatt tgcggcattt gcactatcgg caaatagagc ttgagcttct
     1381 ttgcgagtga gcgatttttc tttgccttta tctcgccagt ttaggcgatt gtaataaagc
     1441 tcgtctaatg taatgggggt attggaaata ctcccttttc ttgtgccaaa agtattatgt
     1501 ttataagcca atggattaat gattttggta aaaatacggc gaacttctac ttcggtattt
     1561 ttgggtgtgt tttggataac aagatcggta aacgccgttt tcatactttc aaacgtattt
     1621 ttattaggct gggcaaaaaa atcggcaaat tttggataaa taccactgtc ttttaataat
     1681 ttttcactaa atgcttggat gaaatttagg gcatttcttt cacgattggc aatatattgc
     1741 aggattttat tattctgtac gccgtaccga tagtatctag aaaacttttc ttcgcttaat
     1801 acgccacaat agctcagcat tttaatcggt tgtgaaaaaa atttattgta ttcgctaccg
     1861 gcattttcaa tggatggttt tttaaatacg gcggtggcga tatattcggc ataatctgaa
     1921 taacgaatat cgttaatgct aaatatttct ccaatatttt caatatattc gatgatacat
     1981 tctgctacgg cacataatac atccggggtt actttttgat cggtaaatgt gccgagttta
     2041 tgtgttctga tgtctaaatt taagttagaa aaataatttt ccatattcat tttttactcc
     2101 tctaatcttt tagcaatccg aaaaaaaaca cagaatgatt gtctatattc attgaacgag
     2161 aaccataatt acgggcaaca cggtaaaact gctcaaattc gggagttgca tagtatgcga
     2221 gagctcgttg ggttaggatt ttttcgtgtt ttttcattgt cagtagggca accgagccat
     2281 cagcaatact gttttcaggc aaaaatgctg cccgaggttt gtaagtcaga ttgggaatca
     2341 tcacgacatt tttacggtta ataaatttgc cgacagaaaa aggggcaact gaatccacat
     2401 aactatcata accctcaata tcaataatct cgttgttacc gatattacgg gattttaaaa
     2461 cccgaacatt gccactattc tgtgtatgcg ctttggtaat ttggcgatct cggaatgatt
     2521 gaaatacatt aaattccaat ttttgtgcaa tttgatcaaa ttcactatta cgataaatca
     2581 accaataggg aaaattatca gaaaaaaaat agtttttaca gttttgtatc aacgtttctt
     2641 ggatatggct ttctatcgta atatgttgat tgtctttgtc ggtttgatcg gcaataaagg
     2701 caatcgtctc tatttttacg cctttaaatg ctttctcgcc gtaatcaata atgcggttga
     2761 tattggctgt gataagttgt tgtcgggtta tttgaaactc gggggcgtta atcacacttt
     2821 tgggaacaac caaagccacg gttttactga gcgtcagagc cttatcaata aaaaatgaaa
     2881 aaatattttg cgtatcttta ttggtagcat ttttacggta tagtgccaaa tttttatcac
     2941 ttgccttaac tttgccgtat ggtggattgc cgatgactag atcatagttt tcaggcagcg
     3001 ttgcggttaa aaaatcctga tgaataatat tgaactcaaa tttactgcct aaatttaatt
     3061 ttttcagcag caatgacagg agatttaaac tgtttgagtc aatatccacc aaatcaaaaa
     3121 taactttttc tttgtctgca tatttttttg ccagtagtgg aatgaaatta ccaatgccga
     3181 cagagggttc taaaatccgt aagttttttt tctttttgaa atcaggaaga gcattaacca
     3241 gtccgaaagc aatatcaata cgggtaaaat aagcagccgt ttcgctacgg gcagcattag
     3301 cgtattcagc aatttgcaaa agccgactga atggatatgt gtcaaaatgt tgctcaataa
     3361 atcggtagag attatcggca agggataaat tttctcgttt gattaaattt tgaatttccg
     3421 tcattgacag ttcggactgt tgcaaggctg cctgaatgtt ttgtgcgaca ttggccaaaa
     3481 cagcggttgg cacagattca ccgatacaat ggcggatatt cagctcttct tttttcaaaa
     3541 aggttttttt ctcatcaaat gacagtgcat taagctgttt ttcatcttgt gccgtccatt
     3601 taaatgatgg cggaattgac atcatacgca tgagttcacg cacggaaaat acacggtttt
     3661 cagtgggatg gatggtgttt tggctggcta aaatgtcatt acgggtatgg atgcacggac
     3721 cctcacgatc taaaaaccaa cgggcgtatt tgtctccatt tttgttttga ttgtagataa
     3781 tttcaccatt tttaatttga tggggaatgc gtgcaggatc agtattttgg aaagctgact
     3841 cgccttcttt aagctgtgca atccacggca acatacggcg atcaaattca cgatagctat
     3901 ggtaaatatc atcatcgcta atttcgccca tttgcattaa aggcggcaga tcagccagca
     3961 atgtacgcaa ggtttttggc ggtgtttgct tggggaaaat atcgtacggc gtaatgtgat
     4021 gaatatcttt tcgcacgcca atcaccagcg ttctggtacg actggaattt gcaccataat
     4081 ctttaaaatt tagtacattg gatagaatgt tgtaatgtcc gcccaaattt gaccaaatgg
     4141 cttcctcaat cggcttttct tgatgatcgg tatcggtgca gggtgtattt aaaaaggctt
     4201 tgacgttttc aaaaataaaa aacttgggct gaacttcccg aacgattttg attgattcca
     4261 cgaccaaaga atttcttgcc atttcattat tttttttatg gttggcaacc gacattcctt
     4321 ggcaaggcgg tgtagcaatc acaacatcaa tttcagtatt ccagcgtcga atttcggcaa
     4381 acagtttggc ttgggtttca ggcagggtaa tatcgccagc aatataacct gaatcaaagc
     4441 ggcatttttg attatatttt tggattttta ggcgtttttc taaaagttcg ttggttgcta
     4501 cgcattcaaa accattggtt ttaaaaccat agcaaccaac gcctgcactg ctaaataaac
     4561 tgatgtaggt gggttttttg ttcatattta tgatgagttg aaacaactgc tctgcaaaaa
     4621 atgcgtgtat tttatcatag aaggcaaggt aaaacacgcc atgcaatgat ttactatatt
     4681 gatttttcat caaaaaagtg ctgtttgaac agatatacag cggtgttttt tgcaatgcgt
     4741 ttcaaacttg aggtacgttt ttaatagaaa caccgtctga acgccaacct gagacctttg
     4801 caaaattccc acaaatcccc caattcccac caagacattt aggggatttc tcatgagcac
     4861 tttcttccg
//
LOCUS       DAXHKX010000147         4864 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHKX010000147 DAXHKX010000000
VERSION     DAXHKX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.147"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(210..938)
                     /gene="rmpM"
                     /locus_tag="ACFMWZ_001724"
     CDS             complement(210..938)
                     /gene="rmpM"
                     /locus_tag="ACFMWZ_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC5935650.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1302..2252
                     /locus_tag="ACFMWZ_001725"
     CDS             1302..2252
                     /locus_tag="ACFMWZ_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC5935651.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2365..3099)
                     /gene="fnr"
                     /locus_tag="ACFMWZ_001726"
     CDS             complement(2365..3099)
                     /gene="fnr"
                     /locus_tag="ACFMWZ_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC5935652.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3302..4723
                     /gene="hemN"
                     /locus_tag="ACFMWZ_001727"
     CDS             3302..4723
                     /gene="hemN"
                     /locus_tag="ACFMWZ_001727"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC5935653.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1241 a         1331 c         1178 g         1114 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tccgaacctg caccccgatt ctgacgatag
       61 ccgtttcaga aaaccaccca aaaaaacaaa ccccgcaaaa gcgggggttt gtttcacatc
      121 atcgatgccg accgacgttt cacgaatatt gcaacagtac ctggtacaaa atccactaac
      181 ctcatgcatc ggcaagatat tgcctagcct tagtgttggt gatgattgtg tgccggcaca
      241 acctgacggg ttacgatgct gcggattttc acatccacac ggcggtcagg ttcgatacat
      301 gcaatcagag cctcacgttt tttggcttta gagactttcg cacccagttt ggcaacttcg
      361 gcttcacaaa cttgagtcat ttgcgcttga gattcgccca agccgacagc agaaattcta
      421 gaaacaggta cgccgttgct gaccaggttg tttgccacta cgtatgcgcg gcgttcggac
      481 agggcctgat tgtatttgtc agaacccata aagtcggtat ggccttcgac gcggacagat
      541 tggatattgg tttgacccag gcgttgcgcc aatactttca ggttgtcttg agcttcggcg
      601 cgcaatgaat ccttatcgaa accgaacagg gttttggcag acagggaaat ggtttcatca
      661 acatattgcg gagcctgctc cacaacgacg acaggagcgg gtgcgggttc gggttctggc
      721 tcgggttcgg gggcagcaac cgcatcgccg cattctacgc gaccttggct tgctttatca
      781 aagtaggcgt ttttccagca ttcgccatag ttgttgcgta cgatttcgtt cgactggccg
      841 cttacggtgt aaccctgaac ggacgcctcg cccgcaacag cagtgccgga agcgagcaat
      901 gcaacgaata atgcgcttaa tttcagctgt ttggtcattt tattccctca ttaaatttgt
      961 acagcagata cagaagcaac tgctgaattg ccaacataca ggccgctgta gaaacatggc
     1021 ggcaaatcgg atatttcagt ctccactata aagaagcagg gggaggactg tcaatactca
     1081 acgcctgaaa acaaccgtaa aacagcctgt ttcctgatag tctatcatgc cgaaaaatca
     1141 attccgtgtc attactttag ggggattttt tccatatcgc acgattgccg ttgcacaaaa
     1201 acaacaaaca caacggcaaa gccccatacc gtaccggcaa gaaaatatga taaattataa
     1261 acaatgttca gccacccgac acagacccac accgacccgc catgaaatta caacaattga
     1321 aatacgcctt agaagtttac cagcacaacc tgaacgtttc cgaagcggcc gaagccttgt
     1381 ttacttcgca acccggcatc tccaaacaaa tcaaattgct ggaagaagaa atcggcattc
     1441 agatttttat ccgcagcggc aagcgcgtgg tttcggtctc gcagccgggc aaggtggttt
     1501 tggatattgc ggaacgtatt ttacgcgatg tacagaacat taaaaatatc ggcagcgagt
     1561 ttaccggaca ggacagcggt tcgctgacgg ttgccacgac gcatactcag gcacgctatg
     1621 ccctaccctt gattgttgcc gattttgtga aacgctatcc gaaagtcaac ctgaccatca
     1681 aacaggggag ccctgccgcc atcgcacgta tggttacctc aggcgaggcg gacttggcga
     1741 ttgttacgga acggatagac gaccatcccg aactgggaaa acttccctgc tatgactgga
     1801 ctcatgcggt tatcgtaccg aacgaccacc ccttgctcga atgcagaaac cccctccgca
     1861 ttgaagattt ggcgaggttt ccgctgatta cttatgaatt tgcattcaat gcgggcagca
     1921 gcatcgcgcg ggcattttcc aaagcccgtt tggaacagcc ggatgtcgca ttggcggcgg
     1981 cggatacgga cgtattgaag acttatgtgc gcttgggttt gggcgtggga ctgatggcga
     2041 aaatggcgta caacccggat acggacggcg atttgcagct tgtggatgcg gcacacctgt
     2101 tcgagccgtc gccgacgtgg attgctttgc gcagcgatac ttatttgcgc ggatatgcct
     2161 acgactttat ccaagcgttt gcgccgcacc tgacacgcga gaaggtggat aggattctgt
     2221 acacgcccat cagcgaggat ttttcgattt aggcggctgc cggttttcag acggcacttt
     2281 gcggcagata caacaaacag gacagatgtt ttcgtctgcc ctgtgtttat tgagaatgcc
     2341 gtctgaaatg ttcgtacggg ttaatcaaat ggcgtgcgaa caaccggata ccatcttctt
     2401 caacacctgc agattgagga tcttgatgtg tttgtgttcg acggaaatca atccttcctg
     2461 atgaaattta gataatgtgc ggctgacggt ttcaagtttc agcccgagat aactgccgat
     2521 ttcttcgcgg gacattctta agatgaagtc gttggcagca aaacctcggg aataaaggcg
     2581 ttgggaaagg ttcaacagaa aggcggcaat ccgctcttcg gcgcgcatat tgcccaacag
     2641 cagcataaca ccttggtcgc gcacgatttc acgactcatc atgcggaaaa agtgggtacg
     2701 caggctgggg atattctgcc ccagttcttc gatgtgggta aacggcagtt cgcacacttc
     2761 gctgtcttcc aaggcgaccg cgtcgcaact gtgcacatgg gaacagatgc cgtccatgcc
     2821 gatgagttcg cccgacataa agaaacccgt tacctgatcg cggccgtcct gactggcgac
     2881 ggttgttttg aagaagcccg aacggatggc aaagagcgag gtaaaggctt cgccgacaca
     2941 gaacaggtat tcgccctttt tcaggcggcg gctttgacgg atgacggcat cgagttggct
     3001 gagctcgttg ggcagcagcc cgacaggcag gcagagttcc cgcaaagaac aggaagaaca
     3061 cagcgttttc atctgatgtg tagtattatg cgaagccata ccgtaccttt ttgtgcgctt
     3121 tgccccatca tgattatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgtc
     3181 cttatttaaa tttaatccac tatatgtgct tattgacaca tatcaagaca ggtttatcat
     3241 actgtggcat tctaccaaac tatccaaaat ttgactaaca tcataaaacc gcacacacac
     3301 catgaaaatc attcagatac agaacaatca caatgtaaac gatgaccgcc ccgagtttga
     3361 ccgcgcgctg attgccagcc tgcccgccag cggcccgcgc tacacttcct accctaccgc
     3421 cgaccgtttc catgacggtt tccgcgaagg cgaatatatc aaagctttac atttgcgcgg
     3481 tatgggcgcg ttaaacaaac cgctttccct ttacattcac attccgttct gcaacaccat
     3541 ctgctactac tgcggctgca acaaaatcat caccaaagac aaaagccgcg ccgatgccta
     3601 catcgaatat cttgaaaaag aaatggaact gctcgctcca catctgaacg gacggcacca
     3661 gcttgcccaa ctgcacttcg gcggcggcac gccgaccttt ttgagcgacg aacagatcga
     3721 acgtgtcttc cgcatgatac gcaaacattt cgagttaatc cccaccggcg aatactccat
     3781 cgaaatcgac ccgcgcaaag tcagccgcga caccgtcctc atgctcggca gactcggctt
     3841 caaccgcatg agcatcggca ttcaggattt cgaccccaaa gtgcaggcag cggtcaaccg
     3901 catccaaagt tacgaagaaa ccaaagaagt catcgatgcg gcgcgcgaag cggggttcaa
     3961 atccgtcagc gtcgatttga tttacggcct gccgcaccag acttcggaaa gcatcaaaac
     4021 caccatcgat accgttttgt cgctcgatcc cgaccgcctc gccctttatc actacgccca
     4081 cctgccgcac gtgttcaaac cgcaacgccg catcgatacc gcctccgtcc ccgacagcga
     4141 agagaagctc gatatgctgc aatactgcgt ccaaacccta accgaacgcg gctacgtctt
     4201 catcggcatg gatcatttcg ccaaacctga cgacgaactc tccatcgccc tcaaagaagg
     4261 cttcctccag cgcaacttcc aaggctattc gacctacgcg gattgcgatt tggtcgccat
     4321 cggcgtgtcg tccatcggca aaatcggcag cacctattcc caaaacgaac gcgacatcga
     4381 tgcctactat gccgccatcg acgaaggcag actgcccatc atgcgcggtt atcagctcaa
     4441 tcaggacgac atcctgcgcc gcaatatcat tcaggatttg atgtgccgct tcgcgctcga
     4501 ctaccggatt tacgaaagta tgttcggcat cccgttcgac cgctacttca aagacgaact
     4561 ggcggatttg gaaaaactcg ccggcttggg attggtgcgc ctgaacagcc acgggctgac
     4621 cgttaccccg aaaggacgct tcctcatccg caacatcgcc atggtgttcg actaccacct
     4681 gcgccacaag gaaaccaagg cgaaatattc gcaaacggtg tgattgcgtc taacgtacaa
     4741 atgccgtctg aaaggctttt tcagacggca ttttgctgcc ggcaggataa gtgttttcaa
     4801 gaacaggcgg cggcatatca taacgttccg cacctttgtg tccgaccgtt ccgaaaccaa
     4861 gata
//
LOCUS       DAXHKX010000148         4839 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHKX010000148 DAXHKX010000000
VERSION     DAXHKX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.148"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            98..904
                     /locus_tag="ACFMWZ_001728"
     CDS             98..904
                     /locus_tag="ACFMWZ_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC5935654.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKQMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(962..2227)
                     /locus_tag="ACFMWZ_001729"
     CDS             complement(962..2227)
                     /locus_tag="ACFMWZ_001729"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC5935655.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPSVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYAEAIAPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGHHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            2634..3344
                     /locus_tag="ACFMWZ_001730"
     CDS             2634..3344
                     /locus_tag="ACFMWZ_001730"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC5935656.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            3417..3878
                     /gene="recX"
                     /locus_tag="ACFMWZ_001731"
     CDS             3417..3878
                     /gene="recX"
                     /locus_tag="ACFMWZ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC5935657.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            3952..4113
                     /locus_tag="ACFMWZ_001732"
     CDS             3952..4113
                     /locus_tag="ACFMWZ_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935658.1"
                     /translation="MSGQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            4266..4748
                     /locus_tag="ACFMWZ_001733"
     CDS             4266..4748
                     /locus_tag="ACFMWZ_001733"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC5935659.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
BASE COUNT         1272 a         1426 c         1100 g         1041 t
ORIGIN      
        1 aaaagacagt tttcagacag caaatccgtc ttcacacgat acctattttg ttataacata
       61 acaaaatctt taacccacac gagacaaagg ctgcaccatg aagaaaacat tgacactgct
      121 cgccgtttcc gccctatttg ccacatccgc ccacgcccac cgcgtctggg tcgaaaccgc
      181 ccacacgcac ggcggcgaat accttaaagc cgacttgggc tacggcgaat ttcccgaact
      241 cgaacccatc gccaaagacc gcctgcacat cttcagcaaa ccgatgcagc tggttaccga
      301 aaaaggcaag gaaaacatga ttcaacgcgg cacatacaac taccagtacc gaagcaaccg
      361 tcccgttaag gacggcagtt acctcgtcat cgccgaatat cagcctactt tctggtcaaa
      421 aaacaaagca ggctggaaac aggcgggcat caaacaaatg cctgacgcaa gctattgcga
      481 acaaacccga atgttcggca aaaacatcgt caacgtcgga cacgaaagcg cggacaccgc
      541 catcatcacc aaaccggtcg gacaaaactt ggaaatcgtc ccgctggaca atcccgccaa
      601 cattcacgta ggcgaacgct tcaaagtccg cgttctgttc cgtggcgaac cgctgcccaa
      661 tgccaccgtt accgccacct ttgacggctt cgacaccagc gaccgcagca aaacgcacaa
      721 aaccgaagca caggctttct ccgacagcac agacgacaaa ggcgaagtgg acatcatccc
      781 cttgcgccaa ggcttctgga aagccaatgt cgaacacaaa gccgacttcc ccgatcaaag
      841 cgtgtgccaa aaacaggcga actactcgac tttaaccttc caaatcggcc attcgcacca
      901 ttaatcccgc ccgcacaaaa atgccgtctg aaggcttcag acggcatttt ttgttcaaac
      961 atcaatacca accgcgcagt ttcatcgctt tttcaacacg gcggatactc atcatgtaag
     1021 acgcggttcg caaatcgaca tcatactctt gcgccaagtt ccatatatcg cggaacgcac
     1081 ggcgcaggac gacggtttct ttttcctgaa cttcgtcaaa ctcccaataa tagccttgca
     1141 gattttgtac ccattcaaaa taagatacca ccacgccgcc gcagttcgcc aaaatatcgg
     1201 gcacgaccaa tacgccgttt tgacgcagga taacgtcggc ttcgggcgta gtcgggccgt
     1261 tcgcgccttc gaccacgatt gtcgcgcgga ctttaccggc attttcagaa gtcagttggt
     1321 tttccagcgc gcaaggggcg agtacgtcca catccaaagc caaaagttcg gcgttggtaa
     1381 tttccttgcc gtaaccggct tcgttggtga tgaagccttt ttcttggaac tctttgaaca
     1441 gggcttccat atccaaaccg ttttcgttgt aaatggcaac gtcaacagta gaaaccgcaa
     1501 caactttcgc gccggattga tgcgcgtaat aacctgtgtg gtgacccaca ttaccgaaac
     1561 cttgaatggc gtaagtggca cccttcacgt ccttgcccag tttttccaag gcttggacgg
     1621 cggcgaggtt cacgccgtaa ccggtggctt cggtacgcgc caaagagccg ccgaactcga
     1681 ccggtttgcc ggtaaatacg cccggcgcgg aatgtttcac cacgttttca taggcatcca
     1741 ccatccacga cataattttg ccgttggtgt tcacatcggg ggcgggaata tcgatttttt
     1801 caccaatcag cggggcaatc gcttcagcat aagcgcgggc gatgcgttcc agttccgcct
     1861 cggaataatc gcgcggatcc aaggtaatgc cgcctttgcc gccgccgtaa ggaatacccg
     1921 caacgcagca tttgatggtc atccaaattg acagggcttt gacttcgtcc aaattcacac
     1981 tgggatggaa gcgcacgccg cctttatagg ggccgacggc gttgttgtgt tgcgaacggt
     2041 agcccgtgaa ggttttgacc gtgccgtcgt cgagtttgac ggggaaattg acttccaaca
     2101 cgcgggtcgg actcttcagg atttcataaa cggccggatc ggttttcagc cggtcgcagg
     2161 cggttttgac ctgtttgcgc gcgatttcaa acggattgag ggtttctttt gcaagggctt
     2221 cagacatttt gcttcctttt cacaaaaaga ttttcagggg cgtgcatttc gctcattact
     2281 ataaccaatt tcccgacaaa atgtaatagc gtgtagtcgg cataagcccg atttgattaa
     2341 tctatatatg attttatttt tcaagccgca cggaatcagc ccggacttcg cctgaaaaag
     2401 cagcgcgcgc gtccgaaaaa taaatgtcaa attaaataaa gatataggca tccttttatt
     2461 ttttaaaaat ttaattggaa catcgctggg atttcaaacc cttgcccgat tccgccccga
     2521 aatccgaaaa caccgcctgc aaaacctgtt gcgattgtta acaatctata cattagaagc
     2581 cctgtgcaaa cgatgttaaa ataaaccctt tcaacccgac aggaaatcag attatgaatg
     2641 cagccatcga acacgtccaa gccgtcgcct tcgatttgga cggcacactg tgcgattccg
     2701 tccccgacct tgccgccgcc gcagaagcga tgttggaaca actcggtatg aaaccgctgc
     2761 ccgccaaagt ggtcgaaagc tatgtgggcg acggcatcgg caaactggtt caccgcgtcc
     2821 tcaccaacga ccgcgaccgc gaagccgatt ccgaactgtg ggaaaaaggt ttcgtatcct
     2881 atatgaaata ctaccgcgac catttgagcg tcttcacccg cccctatccc gaaaccgaag
     2941 ccgggctggc attgctcaaa tctttgggca tcccgctggc agtcattacc aacaaaaacg
     3001 aaatccttgc cgtcgaactg ttgaaacagc tcggacttgc cgactacttc agcctgatac
     3061 tcggcggcga cagcctgccc gagaaaaaac ccagccccct gccgctgcgg cacgccgccg
     3121 aagttttggg tatcgatgtt gcaaacatgg ttatggtcgg cgactcgcgc aacgacatca
     3181 tcgccgccaa agccgccggc tgcctgagcg tcggcgttac cttcggttac ggcgatatga
     3241 cgctgctctc gcaagacgat gcgacccgcc ccgactggat tatcggctcg ctgcccgaaa
     3301 tttacgaaaa cctgcaaccg cagaaaaata aagacgaaga ataaaacaat ctgttcagac
     3361 ggcatcggaa tccgtttccc atgccgtctg aatctttccg tgccgaaacc gccgccatga
     3421 aaccgcaaaa atccctacgc gcccgcgcga tggacatcct ctcgcgccaa gaagtcagcc
     3481 gcatcggtct gaaacgcaaa cttgcaccgc acgccgaaag cgaagaggag ttggaaaacg
     3541 tgttaaacga atttgccgaa cgcaactggc agtcggattt gcgctatgcc gaagcctata
     3601 tccgcagcaa aagccgcaaa cacggttcat tgaggctgaa acaggctttg gcgcaacagg
     3661 gcatagatga agaaaccagc cgcaacctgc ttcccgaccg ctcaagcgaa aaacaggccg
     3721 ccatagccgt gttgcgtaaa aaattcaaac accccgccgc cgacctcaaa gaaaaacaaa
     3781 aacaggcgcg tttcctcgcc tatcgcggat tcgatgccga taccgttcag acggcattga
     3841 aacacgcttg ggacgaaaat tgggaagacg gctgctgacc ttccttcttg aatctttttg
     3901 cacggcaaca taaccttacg cccgtttcca acttttccga ttgagaacaa aatgtccgga
     3961 caacccgaaa aaaatcataa ccccctcctt gaggacgaac gcaagaaccc cgtgtaccgc
     4021 atggggcagg cagttgccgg attcatgctc gtcgtttggg caggcgtatt ggcactcgtg
     4081 tttttcctgg tcttccgttt ttggctttcc taaacaaaat gccgtctgaa ggcttcagac
     4141 ggcatcggcg gcccatttct gcagactatc ccgccatagc tttttttagc ttgaattcca
     4201 cttccccatt ccctaaaatt ttcccacacc catttcaaaa caccctttct taaaacaggt
     4261 acactatgac acaacaacgc caactgcctt cccacgaact cattatgtcc gaactgatga
     4321 tgccggacac cgccaatttc agcggcaacg tacacggcgg cgaactcctg ctcctgctcg
     4381 accaagtcgc ctattcctgc gccagccgtt acagcggcaa ttattgcgtt accctgtcgg
     4441 ttgacaaagt cctgtttaaa gaacccatcc acatcggcga tttggttacc ttttacgccg
     4501 ctgtcaacta cacgggacgc acctcgatgg aaatcggcat ccgtgtcgaa gcacaaaaca
     4561 tccgcaccgg cgaaatccgc cataccaaca gctgctactt cacgatggtt gcggtcaaag
     4621 acggcaaacc cgtccctgtc cctccgctgg aaatcctgac cgaccgccaa cgctgccgct
     4681 acgaaaaagc caaaaaacgc agagacatca gcctgcaagc ctctgaagac atgtcctgcg
     4741 gctgctgacg gcggactatg ccgtctgaaa gacaggcaca tcgcgccatc cgtttccatt
     4801 gcaaacggat gaaatcaagc aaatatagtg gattaaatt
//
LOCUS       DAXHKX010000149         4823 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHKX010000149 DAXHKX010000000
VERSION     DAXHKX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.149"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..485)
                     /locus_tag="ACFMWZ_001734"
     CDS             complement(<1..485)
                     /locus_tag="ACFMWZ_001734"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC5935660.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NA"
     gene            complement(482..1903)
                     /locus_tag="ACFMWZ_001735"
     CDS             complement(482..1903)
                     /locus_tag="ACFMWZ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC5935661.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAVYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(2098..3207)
                     /gene="ribD"
                     /locus_tag="ACFMWZ_001736"
     CDS             complement(2098..3207)
                     /gene="ribD"
                     /locus_tag="ACFMWZ_001736"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC5935662.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            complement(3240..3704)
                     /locus_tag="ACFMWZ_001737"
     CDS             complement(3240..3704)
                     /locus_tag="ACFMWZ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC5935663.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTKHRLYTSGQRD
                     IPSAALADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            complement(3733..4575)
                     /locus_tag="ACFMWZ_001738"
     CDS             complement(3733..4575)
                     /locus_tag="ACFMWZ_001738"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC5935664.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT          996 a         1217 c         1626 g          984 t
ORIGIN      
        1 gcgttgtagg cgtagacaat ccggtcggcg gggaaaccgt ggcggatgag tttttcgcgt
       61 tcgtgtttgc agttaccgat gatgtacacg cccagcttgt cgaagagttt ggcgattttg
      121 gggtaggttg ccgcgtccaa accgtgcgag tggtagatga ttttggtttt cggcgaaacg
      181 attttggcgg caatggcgca ggcgggtacg acgcgcgcca tttggcagtc gatgatgtcg
      241 ggttgttcgc gccgcagcat tcgggcgtag gcaatcatac ctttgaggta gccgaaaagg
      301 ccgccgcgat agaaatcgac gggttgccaa cgtatgccca aggcttgcgc ctctttgagc
      361 aacggtccgt ccgaagaggc aagcatgatg tcgtgtccgt gctgcctgag tagcctgccg
      421 aggcggacgg tggcggtttc cgtgccgccc aagcctgcca tagatgtggt caaaacgatt
      481 ttcataatgg gaaaccttgt tttttcaaat aatgaaacag tttgtgcaaa tctttccggt
      541 ggcgcaggat gcagcctgcc agatataccg cccatacgcc ggcaaacagg gggtagtttg
      601 ccggcgtgcc gaagcaggtg taggccgccg aggaggccag gcagaacaat gtgtgcatat
      661 aaagcggcag gcgtttgagc ggctgccaca ggcggcagga gctttcggtc ttgaaaacaa
      721 aaaacagcca aaatgaggcg gcacaggcaa ccgccgcgcc gcgcgcgccg ccggacggta
      781 cggcaagccc cagcagcagc aggtttgccg ccagcgcgcc caaggtggcg agcgcgatcg
      841 ggcgtgtttt tcggacgacg ttcaaaccga tgccgctgat ttctaccagc gtgcaaaaca
      901 gcggcggcag catacacgat acgacgataa accggacggc ggcgtagttt tccggcagca
      961 ggagggaggc gaggggcgag aaaatgccgg tcaggcagag ggcggaggca agcagggcgg
     1021 cggcggattc tgccgttgcc gagaggcggg cgggcggggc gtttgcttcg attgcgcgga
     1081 aaatatacgg tgtccagacc gttgaaaaga tgctttggaa caataatgcc gccccgccga
     1141 acgaaatacc catcgaataa acgccgagct gttctaggcc ggcatatttt ttcaggaaca
     1201 aacggtcggc ggatgccagc ccccaatagg cgatgctgct tagtgcgatc ggtatgccgt
     1261 agcgcaggcc gcgatgcagg acggcggatg aaaacggtgc gcgccggacg gccttcagac
     1321 ggcatcggtt ttgaaacagc aaaaaggcgg cggcggcaag gtttgccagc gcgtaaacgg
     1381 cggtcaggac ggcggtgttc gccggaaagt gcagcagccc gaccgtcagc ggcagcagca
     1441 gcaggatggc gagcttggac acgagttgcg cggacgaaaa ggcaagggcg cgtccttcca
     1501 tacgcaaaac cagtaagaga aagcggatgg gcaggaagct cagttcaaac agcaccagcc
     1561 cgatgccggc ggcggcatcg tcgagcgaaa acaggatttc agacggcagg gatgggcggg
     1621 aaagcagcag ggcggctatc gcggcggcag acagcagcgg cggcaggaac agggttttga
     1681 acaaggtgtc tttgtcggcg gcggcatagt attcgcggac gtatgcctga tccagcccga
     1741 ggcacaatac cgacaccgtc agtcccgccg ccgtctgcat cagcacgatg cgcccgatgt
     1801 cgtcggcggg gaaataccac gacagcagcg gcaggatgat gacggctaaa accgcgctgc
     1861 cgatcgagcc tgccgcgtag ccgaggattt cttttgtgtc catttttgat gtccgggcgg
     1921 cggcgggatg ctgcctgtgt cgtctgaagc ctttcttgat cggaatttga cggctttcag
     1981 accgtcgcgg ctgccggcgg ggtgcggcag tccgggtcgc gttttttcgg gtgggcggcg
     2041 gtctgaatgg tgccgttttt tatcggcgtt attatatagt gaaacggcgc aaacccttta
     2101 aaaggcgttg ccgttttttc ggaacacggt tttgatgtcg tgtccgagga tttcgcttga
     2161 aacgggtgtc cacaagggcg gtgcggaaag ggcggcgcgg ttttcgagca gggaaaacag
     2221 gtctttgccg ctgccgagga ttttgggcga acggtacagc acgatttcgt ctgccagatt
     2281 ttctgccaaa aatgcggatg tgagttcgga gcctgcttcg accatgattt cgccgaaacc
     2341 ttcgtcagca aggaggcgca tcaggtggtg caggtcgatt ttgctgtctg ccgtttcaga
     2401 cggcatcagg atgcggacgt gtgcgtgttc ccgatagggg tgcagtctgt cttcgttgcg
     2461 ttcgagtgtg gcgatgtagg tcggagattg tccgtcggta accaaatggc tgttcggggg
     2521 caggcgcagg cggctgtcta aaacgatgcg tgcgggttgg cgcaaagttg gaaaagcgcg
     2581 gacgttgagc cggggattgt ccgccaacac cgtgccgatg ccggtcagca ccgcgcagct
     2641 ttcggcacgc aaaacctgta cgtcggcacg cgcgtcttcg ccggtaatcc aaaagctgct
     2701 gccgtctgaa agggcggttt tgccgtccag cgaaacggcg catttgaggc ggacgaaggg
     2761 gcggcggcgt tcgatgcgcg acaggaagcc tcggttgagt tcccttgcct gatgttcgag
     2821 taaaccgcat tccgtcttga tgcctgctgc ttcgagcagg gcaagccctt tgcctgcaac
     2881 cagcgggttg gggtcgcgca tggcggcgac gacgcgtgtt acaccggaac ggagcagagc
     2941 ttcggcgcag ggcggtgtgc gtccgtaatg gctgcacggt tcgagggtaa caaaggcggt
     3001 cgcgccttgt gccatttcgc ccgcctgacg cagggcgtgg acttcggcgt gcggttcgcc
     3061 cgctttgacg tggaagcctt gcccgacaat ttggctgccg tgtgcgataa cgcagccgac
     3121 gcgcggattg ggcgaagtgg aaaaacgtcc caaagcggca agtcggaggg cggtttccat
     3181 catggatata tctgtgtccg aaaacatagg gataccgtat cagtatgggt tggggagaat
     3241 caggctttgc ggtctgtttt gacggcttgc gccagccacg aggcaaagtc tgccggattg
     3301 tcgaagcgtt tgtgcagggc ggcgaaacgg acggcggctt ccgtgttgtg ctcaagcagc
     3361 tcttttagca ccatgtcggc aagtgcggca gaggggatgt cgcgctgacc cgaagtgtag
     3421 agcctgtgtt tcgtcaggcg gacggtttcg tcgatctgtt cgggtgtcag ggcggatttt
     3481 cgggcggcgg cggtcaggtc gttgcggagg cgttgtgcat taaagggcga acgttttttg
     3541 tccggaccga tgacggcggg cattttgagt tcggcggttt cgagcgtgcc gaagcgtttg
     3601 ccgcagctgg ggcagtggcg gcggcggcgc acggcgttgc gttcttccat caggcgcgaa
     3661 tcggcgacgc gggtgtcggg gtgggcgcaa aacgggcatt tcatagcgtt tcttcctgat
     3721 gccgtctgaa cgtcaaaccg atacgccggc ggcgcgggcg atttccagac cttcttcggc
     3781 actcatatag acggggtttt cgggacggtc gtgtcggacg atgttgcctt cgcggaacat
     3841 gaccagttcg tccacggcaa gttgggacca tgattcgtcg cgggtcagtg gcagggtggc
     3901 gataacggcg acgcggtcgg agggcgtggt tacttcggca aaatcgacca tcacgtcgtc
     3961 gtcgagcagg cgcgccttgc cgaacggggc ttggcggacg atgtagtgca gcagtgtgct
     4021 ggcgtgggca aacagggcaa tgccgtctga aagcataaag ttaaacagcc cgaacttgcg
     4081 gatttcgtgc gtcagccccg caatcgcgtc aaacagcgtg tcgtcgtcgg gacgggcggc
     4141 aaagcgggtg cgcaggcggt tgaggatgtg gcagaacgcg cgttcggaat cggttgtgcc
     4201 gacggggtgg aaaaattcgc cctgttcggg gaaaaaatca atcaaatgtc cgttgtgggc
     4261 aaacagccag tagccgcccc acatttcgcg cataaaggga tgggtgttcg ccagcgaggt
     4321 ttgtccttgc gatgctttgc ggatatgtgc gatgacgttt tccgatttga tttggtaggc
     4381 acgcacgagg tcggcgacgg gggaatttgc gctcggcttg tcgtcgtgga acaggcgcac
     4441 gcctttgcct tcgaaaaagc cgatgccgaa accgtcggca tgatggtcgg taatgccgcc
     4501 gcgccggcgg aagccttcaa aggaaaacat aatatcggtc ggcgtattgc agttcatgcc
     4561 cagcagttga cacatagttt gtccaaatga ttcagatggt cgcaagtatt cggattatac
     4621 cccgaactga aaatgccgtc tgaaatacgg cttgttcccc attattccca cgaaaacaga
     4681 aaacaaagac ggaaacttaa gattccgtca ttcccgcgca ggcgggaatc tggaatttca
     4741 atgcctcaag aatttatcga aaaaaaccaa aacccttccg ccgtcattcc cacgaaagtg
     4801 ggaatctaga aatgaaaagc aac
//
LOCUS       DAXHKX010000150         4792 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHKX010000150 DAXHKX010000000
VERSION     DAXHKX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.150"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(299..2575)
                     /gene="znuD"
                     /locus_tag="ACFMWZ_001739"
                     /gene_synonym="tdfJ"
     CDS             complement(299..2575)
                     /gene="znuD"
                     /locus_tag="ACFMWZ_001739"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC5935665.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDSALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHAHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLALYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2997..3587
                     /locus_tag="ACFMWZ_001740"
     CDS             2997..3587
                     /locus_tag="ACFMWZ_001740"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC5935666.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3651..4709
                     /gene="trpD"
                     /locus_tag="ACFMWZ_001741"
     CDS             3651..4709
                     /gene="trpD"
                     /locus_tag="ACFMWZ_001741"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC5935667.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
BASE COUNT         1065 a         1110 c         1398 g         1219 t
ORIGIN      
        1 cgcgtcattc ccacgaaagt gggaatctag aacccaaatg ctaaggcgat ttatcggaaa
       61 cggctgaaac cgaatgaatt ggattctcgc ctgcgcggga atgatgggtt tctgcttttg
      121 atttttctgc ttttgcgaga atgacggcgt gaaagcaaga atgatgaaac aaagaaaatg
      181 ggaatgatgg catagtggtt tgttctttgt ctttgccata tttcctaaca aattgattaa
      241 aaagaaaaaa ggttttcaga atgccgtctg aaaacctttt ttgtttgcct gtccgatttt
      301 aaaacttcac gttcacgccg ccggtaaagc tgcggcccat ttgcggcgta tcagagagaa
      361 agctgctgtg ggcgtaaacg gattggttga gcaggttgtc ggctttgacg taccaattcc
      421 actcgccata gcgcgtattg cggcggtagt ttgcgccgag gttgagcata tggtgtccgg
      481 gcgtgcgtgt ttcgtagagg gcgagtttgt tttgggcgaa cacgcggtag tagtctaaat
      541 tggcatcgat gcggtcggtc agcgaggctt tcaggtggac gccgaggcgc gcagccggaa
      601 cgcgaggggc attttggtcg tcctgtgcga tgaaaggacg gttgccgtag gcatcttccc
      661 tgccgggtag ggacggcagg tttttcagac ggcctcgtac atagtcgccg gaaacgccga
      721 tgcggtagcg cggtgtcggt ttgaagtaga tttcgccttc tgcgccgtag aagtccgcac
      781 cggattggtt gtagcgcacg agcttcattt cgctgtcgtc ttcgatggat ttggggccgc
      841 gtccgtcgtt taaggtttgg gcgtaaatgt agttgccgaa gcggttgcgg tagagtgcca
      901 gattgtattg ccagcggtcg ccttcgtagc ccagcgcgag ttcgatattg ttggaacgct
      961 ctttgttgag gtgtttgttg ccgacttcaa aggtgttggt ggcgacgtgt ttgccgtgtg
     1021 cgtacagctc ttgcgttgac ggcaggcgtt cctgatggga ggcggtcagg ctgagtttgt
     1081 gttgtggcgt gaaataccag ttgcccgaaa gtgcgaatga gcgggcggtt tggcggtgcg
     1141 cgccgaggtc gggcagggga tggttgtagt agttttcccg atcaatcaat gctttgtcgt
     1201 agcggatgga ggctttttgt ttttccacgc gtacgccgcc ttcaagcgtg aagttgtccc
     1261 agtttgcctg ttctacaccg aaaaagctgt aatgttgcac tttattgtca agcagcatcg
     1321 gttgtttgac cgcttcggat gtggcagata aagcactgga tttttgtccc aaatattgca
     1381 cgccccagct gcctttcaga cggcctatgg gttggtggcg caactcgatg cgggcgtttt
     1441 gcgtttggtt gttaaaaaag ttttcgactg catcgcctgc tttttcgtcg tggtggtagt
     1501 cgttgcggtt caggtgtacg cgcagggctt caaaaccggg gaatggctgc ttccattcgg
     1561 cgcggagttc gtagcgtttg ttgcgcaggt ctatccaagg tttgccgttg tgggcatggg
     1621 cgtgtgcatc atcgtcgtcg tgaaagccgc agctcaagcc cggattgtcg taatcgatgt
     1681 cttcttcggt caacaggtgc ggataaagct gcaaatagcg tttgttaatc aaactctttt
     1741 gccagatgat gtcggcgtgg caatcatcgt attcgtggct gtgggcaggc agaccatatt
     1801 ggtcgcgacg gtcgctgtat gctgcgccga taaagccttt ttcgccaacc caagacagcc
     1861 cgatgctgcc cgtttgcgaa tcggcgtggc tgtcgggcag gcgtttcaga ttgcggtaac
     1921 gcggtacggc gtaatcgccc gatttgcggt acaagccttc cgtgtgcaat acaaagtttt
     1981 tgcccaaacc gatattgatg ccgccggacg tgagtttttc cagattgccg ctgctcaaac
     2041 gcaatccgag ttcgcccgat acgccgtttt caggcatttt ttcggggatt ttgccatcgg
     2101 caacatcgac cagccccgcc acattgcccg agctgtacaa gagcgtaacc ggaccgcgca
     2161 ggatttcgac ctgttgcgac aaggcgctgt ccaccatgat tgcatggtct ggagagaagt
     2221 ccgccatgtc gcccgtttcg ccgtgatggt tcaacacttt aatccgcctg cctgtttgac
     2281 cgcgaatgac gggagcagac gcgccgccgc cgtattgcga agcgtggatg cccggtacgc
     2341 cgtctaaagc gtcgcccaag ttgacggctt tttggcgcaa ggtatcgccg gagatgattt
     2401 tgtcggaggc ggtcgaagtg tgcaacagcc ccgacgtggc gcgcggacgg cttttgccga
     2461 cgacgctgac cgtttccaaa tccaccgatt gctcagtttc atgcgcttgg gcgaggaggg
     2521 gtgtgttgat taaaagaatt gataaaacaa tgggtttgag tgtagtttgt gccattttgg
     2581 cttctcgtcg catttcaaaa gtttgttatt atataacatt gcattcctta tatcataaga
     2641 ttttgagaac actcagaggg cataggcaaa agtttttcaa atgaaacggt tgcggcatcg
     2701 ggcggtgtcc atttgtatcc gccgtccttc gggggcgcgg ttgatgttga cgcagattcc
     2761 gctgtgtttg cccattatgt tcggctgcgg gataaccaaa atttatgagt gcataaaaac
     2821 ggcacgttcc cgaacggtcg aaaaggtggc aaaatggcgt acttgtcaga acgcgaggct
     2881 ctgcgccagg ttcacgaggg cgcatcgggc acgctaagat ataagagggt atggatgggg
     2941 gtatcggaaa tgcagttaat aacaaacaaa ttataaatca ataggttaat cacaaaatgc
     3001 ttttgtttat cgacaattac gacagtttta cttacaacat cgtccagtat ttcactgaat
     3061 tggggcagga agttgccgtg cgccgcaacg atgatattac gttggaggaa atcgaggcat
     3121 tgaatccgca atatctcgtt atcggccccg gcccgtgttc gcccaaagaa gcggggattt
     3181 ccgtggcggc gatgcgccat ttcgccggcc ggctgccgat tatgggcgtg tgcctcgggc
     3241 atcagacgat aggcgaggcg ttcggcggca ggatagtccg cgccaaaacg ctgatgcacg
     3301 gtaaggtgtc gcccgtgtcc cattcgggca agggtatgtt taagggtttg cccaatccgg
     3361 ttacctgtac gcgttatcac agcctcgtta tcgatcggaa tacgatgccc gaatgtttgg
     3421 aagtaacggc ttggactgag gacggcgaga ttatgggtgt gcgccataag gaatatgccg
     3481 tcgagggcgt gcagttccac cccgaagccc tcttgaccga gcacggacat gatatgttaa
     3541 acaatttttt aatcgaattt caaaacttca aaccgcaaaa aatctgacgt gatgccgtct
     3601 gaagcccttc agacggcatt ttcgtccgaa tattgaacgg aggacaaaaa atgattacac
     3661 cgcaacaggc catcgaacga ttaatcagca ataacgagtt gttttacgat gaaatgaccg
     3721 acttgatgcg tcagattatg agaggacagg ttctgccgga gcagatagcg gccattttga
     3781 caggattgcg tatcaaggtt gaaaccgttt ccgaaattac cgcagctgca gccgtcatgc
     3841 gcgagtttgc gacaaaagtg ccgctggaga atgcagaggg gctggtcgat atcgtcggta
     3901 cgggcgggga tggcgcgaaa accttcaata tttcgacgac ttcgatgttt gttgccgcag
     3961 cggcaggcgc gaaggttgcc aaacacggag gccggtcggt ctcttcctcc agcggtgcgg
     4021 ctgacgtggt ggagcagatg ggcgcaaacc tcaacctgac tcccgaacag gttgcccaaa
     4081 gtatcaggca gaccggcatc gggtttatgt tcgcgcccaa tcaccacagt gccatgcgcc
     4141 atgtcgcccc tgtacgccgt tcgctcggtt tccgaagtat tttcaacata ttgggtccgt
     4201 taacgaatcc tgcgggcgcg ccgaaccagc ttttgggcgt gttccacacc gatttgtgcg
     4261 gcattttgtc gcgggtcttg caacaacttg gttcaaaaca cgttttggtt gtttgcgggg
     4321 agggcggttt ggatgaaatt acactgacgg gcaaaacacg cgttgccgag ctcaaagacg
     4381 gaaaaatcag cgaatacgac atccgcccag aagatttcgg tatcgaaacc cgccgcaatt
     4441 tggatgaaat caaagttgcc aatactcagg aatctttgtt gaaaatgaat gaggtgctgg
     4501 aaggaagaga aggggctgcg cgcgatatcg tattgctcaa caccgccgcc gccctgtatg
     4561 ccggaaatgt cgctgcttcg ctttcagacg gcatatctgc cgcacgggaa gccatcgatt
     4621 caggcagggc caaatcgaaa aaagaggagt ttgtcggttt tcaaccacaa caaagatgcc
     4681 attttcttgg aaagatggag cttgggtgat gccgccatga ttatggaact tttgtggcaa
     4741 aacataagca cttcacgaag agaacttacc aaactgtttt tatataaaaa ct
//
LOCUS       DAXHKX010000151         4726 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHKX010000151 DAXHKX010000000
VERSION     DAXHKX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.151"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            165..944
                     /gene="pgeF"
                     /locus_tag="ACFMWZ_001742"
     CDS             165..944
                     /gene="pgeF"
                     /locus_tag="ACFMWZ_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC5935668.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAESLGGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCMPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            996..1475
                     /gene="lptE"
                     /locus_tag="ACFMWZ_001743"
     CDS             996..1475
                     /gene="lptE"
                     /locus_tag="ACFMWZ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC5935669.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1477..2475
                     /gene="holA"
                     /locus_tag="ACFMWZ_001744"
     CDS             1477..2475
                     /gene="holA"
                     /locus_tag="ACFMWZ_001744"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC5935670.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2596..3015
                     /locus_tag="ACFMWZ_001745"
     CDS             2596..3015
                     /locus_tag="ACFMWZ_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935671.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3090..3605
                     /locus_tag="ACFMWZ_001746"
     CDS             3090..3605
                     /locus_tag="ACFMWZ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC5935672.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3689..4573)
                     /locus_tag="ACFMWZ_001747"
     CDS             complement(3689..4573)
                     /locus_tag="ACFMWZ_001747"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC5935673.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGSQSLELNRDLVGQLAEINQTLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1058 a         1252 c         1323 g         1093 t
ORIGIN      
        1 taagggcatc attcctgcac cggcaagaat ccgaacccga acgtttgaaa acaatcccga
       61 atctccgaat tcccgcctgt gcgggaatga cgaaaaaaca agcatccatt tgccccgaag
      121 gcagttaatc aaccctttcc gccacacacc tattccaata tccaatgaaa accatcacag
      181 aaaccctaaa tctcgccccg aaaggcaaaa acttcctgac cgccgattgg cccgcgcccg
      241 ccaatgtgaa aaccctgatt accacgcgca acggcggcgt gagccaaggt gcgtatcaga
      301 gtttgaacct cggtacgcac gtcggcgaca atcccgaagc cgtgcgccgc aaccgcgaaa
      361 tcgtgcaaca gcaggtcgga ttgcccgttg cctacctcaa tcaaatccac agcaccgtcg
      421 tcgtcaacgc tgcagaatcg ttgggaggca cacccgatgc ggacgcttcc gtggacgaca
      481 cgggtaaagc cgtctgtgct gtgatgaccg cagactgtct gcccgttcta ttttgcgaca
      541 gggcaggtac ggcggttgcc gccgcacacg cgggctggcg cggtttggcg ggcggcgtat
      601 tgcaaaacac cattgccgcg atgaaggttc cgcctgtcga aatgatggcg tatctcggtc
      661 ccgccatcag tgcggatgcg tttgaagtcg ggcaggacgt gtttgatgcg ttctgcatgc
      721 ccatgcccga agccgccgcc gcatttgaag gcataggcgg cggcaaattc cttgccgacc
      781 tttacgcgct cgcccgcctg attctgaagc gtgaaggcgt gggcggcgta tatggcggca
      841 cgcattgtac ggttttggaa cgggacactt tcttttccta ccgccgcgac ggagcgacag
      901 ggcgtatggc gagcctgatt tggctggacg gcaatgccgt ctgaacacgc cgctgatata
      961 atctaccgac tttgtgcttt tgagaaaggc aagccatgaa caaactgttt cttactgccg
     1021 cagtgctgat gctgggcgcg tgcggtttcc acctgaaagg tgcagacggc atttctccgc
     1081 cgctgaccta ccggagctgg cacatcgaag gcggacaggc attgcagttt cctttggaaa
     1141 ccgcgctgta tcaggcttcg ggcagggtgg acgatgctgc cggcgcgcag atgaccctgc
     1201 gtatagacag cgtttcccaa aacaaggaaa cctacaccgt tacccgtgcg gcagtcatca
     1261 acgaatatct tttgatattg acggttgaag cgcaggtatt gaaacgcggc gagccggtcg
     1321 gcaaaccgat gaccgtgtcc gtccgccgcg tccttgctta tgccgacaac gagatcttgg
     1381 gcaaacagga agaggaagcg gcattgtggg cggaaatgcg gcaggatgcc gccgaacaga
     1441 ttgtccgccg cctgaccttt ctgaaggcgg aatgacgtgg cggcacatat cggacgcatt
     1501 gatacggacg cgcctttgaa acccctgtac gtcatccacg gcgaggaaga actgttgcgt
     1561 atcgaggcat tggacgcatt gagggcggcg gcgaagaaac aaggttacct taatcgggaa
     1621 gtttatacgg cagacaatgc cttcgattgg aacgaactgt tgcaaaccgc aggcagtgcg
     1681 ggtctgtttg ccgatttgaa gctgttggaa ctgcatatcc ccaacggaaa acccggcaaa
     1741 accggcggcg aggcgttgca ggattttgcc gcccgcctgc cggaagatac ggtaacgctg
     1801 gttttgctgc ccaaactgga gaaaacccag ctccagtcca aatggtttgc cgcattggcg
     1861 gcaaaggggg aagtgtggga agccaaaccg gtcggcgcgg cggctttgcc ccaatggata
     1921 cgcggacggc tggacaaaat cggtttgggt atcggggcag acgcattggc actgtttgcc
     1981 gagcgcgtgg aaggcaatct gttggcggca cgtcaggaaa tcgacaagct cgcgctgctg
     2041 tatccgaaag ggcataccgt caatatcgat gaggcgcaaa ccgccgtcgc caacgtcgcc
     2101 cgctttgacg cgttccaact ggcaggcgcg tggatgaagg gcgatgtccc gcgcgtatgc
     2161 agattgttgg acggattgca ggaggaaggc gaagaaccgg tgctgcttct gtgggcggtt
     2221 gccgaagacg tgcggacgct gatccggctt gccgccgccc tgaagcaggg gcagagcatc
     2281 caatccgtcc gcaacagcct caggctttgg ggcgacaagc agacgctcgc accgcttgca
     2341 gtcaagcgga tttccgtcgt ccgcctgctt gacgcgctca aaacctgtgc ccaaatcgac
     2401 cgaatcatca aaggcgcgga agagggcgac gcatggacgg tattcaaacg gcttgtcgtg
     2461 tcgctggcgg aataaagcgg taatccccaa aatccgaaaa tactgtaaaa taccgttaat
     2521 cctgaaaagt attcaccaat ccgtccgaaa acatttcaga cggcacgacc acctcaataa
     2581 aggaacatta accctatgga caataagacc aaactgcgct tgggcggcct gattttactg
     2641 accaccgccg ttttaagcct cattatcgta ttgattgtcg attcctggcc gcttgccatc
     2701 ctgcttgcag ccgtcatcgt cgctgccgct gcgggcggtt ttgtttggac atcccgccga
     2761 cagcaacgcc agtttatcga acgcctgaaa aaattcgaca tcgatcccga aaaaggcaga
     2821 atcaacgagg caaacctgcg ccgtatgtac cacagcggcg gacaacacca gaaagatgcg
     2881 attaccctga tctgcctgtc gcaaaaatgt tcggtggacg aggcgcacgc tatgttcaaa
     2941 aaacgcccga cacgtcagga aatcaatcaa atggcggcaa aacagtcgcg cggtcagaaa
     3001 cgtccgcacc gttaaccaca tgagacatat ctgcatgaat gccgtctgaa gcctgttggc
     3061 gtttcagacg gcatattctg attgaaaaga tgatgacact gaaaaccgcc ccgctcaaac
     3121 gccgctttgc cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag
     3181 cattgtttgc cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat
     3241 tggtaacgag tatcctgata atgggatcat ggtggcttta ttttcgcgcc aactggcacg
     3301 gtcaggggca gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca
     3361 tacagccgtc tttgtacctg ctgcgcctgc gctttatttg ggcgtgcata tttatcgtct
     3421 tcattcctat gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg
     3481 cggccggcgc ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg
     3541 atcggcagtt tctgtatgat tttcttgcag gaacaagatt agtggcggtc aaaggaaagc
     3601 cttaagcctt tataccgcaa aggtttcaac ctgaaaaaat gccgtctgaa agggctttca
     3661 gacggcattt gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct
     3721 gttcgatcag gactttcagc tcgaccgagg cttgggtgca ttcggtagcg atggatttgc
     3781 tgcccaaagt gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc
     3841 tgctgccttt gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc
     3901 tgaactcttc gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt
     3961 cgttgtcgat gctgccgacc gcttcgacga ggcgggcgcg gattttttct ttatgtgttt
     4021 ccaacagggt aggaaagagt tcgcttaatg catctatgat ttcttccata gcctcaaggc
     4081 gttgcagcag gtgctcgcct aatttcttac cttcccgctt gcgtgcggca gtaaagtctt
     4141 ttaacgcttt ttcggtcagt tcggtaatgc tttttgccaa ttcttccgta ttttcccttt
     4201 ggcttgccaa tacgccgggg aaacgcagga tgtcggcaac ccctaatttt gccaggtcat
     4261 gatgtttacg gagggtttgg ttgatttcgg caagctgtcc gaccaagtcg cgattcagtt
     4321 ccaaggactg actgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc
     4381 gtgcgatatg ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg
     4441 gcatcctgat ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc
     4501 gtttgctgcc gcactctgcc gccgcgttgg caaatccggt catgctgtgg atgtgaatat
     4561 ttccgctgct catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt
     4621 atggcggcaa gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc
     4681 acgcgctttt gttgctgcaa ggaagggaaa tgtatagtgg attaac
//
LOCUS       DAXHKX010000152         4698 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHKX010000152 DAXHKX010000000
VERSION     DAXHKX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.152"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(416..952)
                     /locus_tag="ACFMWZ_001748"
     CDS             complement(416..952)
                     /locus_tag="ACFMWZ_001748"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC5935674.1"
                     /translation="MDLREVKLGGETIYEGGFVSISKDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(986..1342)
                     /gene="folB"
                     /locus_tag="ACFMWZ_001749"
     CDS             complement(986..1342)
                     /gene="folB"
                     /locus_tag="ACFMWZ_001749"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC5935675.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1398..2000
                     /gene="plsY"
                     /locus_tag="ACFMWZ_001750"
     CDS             1398..2000
                     /gene="plsY"
                     /locus_tag="ACFMWZ_001750"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC5935676.1"
                     /translation="MFNISAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            2088..2933
                     /locus_tag="ACFMWZ_001751"
     CDS             2088..2933
                     /locus_tag="ACFMWZ_001751"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC5935677.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYWRVTETLKSALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3179..4153
                     /locus_tag="ACFMWZ_001752"
     CDS             3179..4153
                     /locus_tag="ACFMWZ_001752"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC5935678.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(4296..4514)
                     /locus_tag="ACFMWZ_001753"
     CDS             complement(4296..4514)
                     /locus_tag="ACFMWZ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC5935679.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            complement(4490..>4698)
                     /locus_tag="ACFMWZ_001754"
     CDS             complement(4490..>4698)
                     /locus_tag="ACFMWZ_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935680.1"
                     /translation="GKAENLCVKRDIVNVFINRTAVLKENFAVSDKTLDKNIIFVIIN
                     VKIQFDFLTVNQERKAICSQNTVI"
BASE COUNT         1213 a         1404 c          985 g         1096 t
ORIGIN      
        1 ctcaaattca aattgggctt ttaggtctat aacaaagaaa aagccttggt aggataaacc
       61 taccaaggct ttttcttaaa aaaacgctct attaccgtgc ctaaaaatgc aaaaaagtaa
      121 ccgtcataac ccttttgcca aaatcaatat catcatccga tgaatgaatt gacggtatat
      181 aagaaagaat taataagctc ataaaccggt aagaaaaatc agtaacaaag atattcatta
      241 ttaaccattt taaaaattat catgttcgtt tgggtaatta ttataccctt ttccctctta
      301 ttactaaagc acgaatgact ttaaaggacg cgcccagaga caagccgaaa aggcatatct
      361 catccgccaa acaaaatgcc caccgactta ctccggcagg cagttcaaca ccctgtcaat
      421 ccttcatcag ccagtattgc aaaccgatta atgtcttgcc gtctttaatt tcatcgtttg
      481 ctaatgcctg acggacttct tctttcgaca tcaatacggt ttccgtaatc tcgtcttcgt
      541 cattggcaag cgtactgccc aaacgcacgc cttccgcttc gaacagatac attttttcgt
      601 tgcaaaaacc gaccgccgta taaaaactgt aaagcaggcg cacgctgtcg gcaacataag
      661 gcgtttcttc agccagttct ctcagcgcac acgctgccat atcctcgccc gccacatcca
      721 gcttgcccgc aggaagttcc aatgtcgcct gatttgccgc ataacgccac tgccgcacca
      781 aaaccacttt cccctcgtcc gtaaccgcca acacgcatgc cgcacccgga tggcggatga
      841 cgatacgctg cccttcattg ccgttgggca acctgacctt atccttgctg atgctgacga
      901 aaccgccctc gtaaatggtt tcgccgccta attttacttc cctcaaatcc acttcatatc
      961 tcctttccgt ttcctattct gccgttcaat cttcacgctt gccgcgctcg atttccacgc
     1021 caacctcgcg cacgccttcc aaaatacccg gcttgacgat tttcacgcgc acccacaccg
     1081 cgccgaaata tcccaaaacc aaatcggcaa tatattccgc caacgcttcc aaaagcagga
     1141 aatcctgttc tttcagatgt cggcgcagcg tttcgcatac ctcggcataa tgcaccgtat
     1201 tggcaatatc gtcgtccgaa cccgctttct cgggaacacc gatgtccaaa tcgacaatca
     1261 gggtctgcaa ccgttcgcgc tcccagccgt acacgccgat aagcgtatcc gccttcatac
     1321 cgtgcaaaaa gattttatcc ataatccgcc ccgttttttg ctaaaataac cgcaccattc
     1381 taagtaaagt accgcaaatg ttcaacatat cggctgttgc cgtttcctat ctgatcggct
     1441 cactttcttt cgccgtcatc gtgtccaaat attacggcat ggacgacccg cgcacctacg
     1501 gatcgggcaa tccgggggca accaatgttt tacgcagcgg caaaaaaaag gcggccgcgc
     1561 tgacgctctt gggcgatgcc gccaaaggtt tagttgccgt tttgcttgca cgcgtgcttc
     1621 aagaaccgct cggtttatcc gacagcgcaa tcgcggccgt cgcactcgcc gcgctggtcg
     1681 ggcatatgtg gccggtgttt ttcggattta aaggcggcaa aggcgtggca acggcattgg
     1741 gcgtgcttct ggcactctct cctgcaactg ccttggtctg cgcgttgatt tggcttgtga
     1801 tggcattcgg cttcaaagta tcctcccttg ccgcattaac cgccacaatc gccgcccccc
     1861 ttgccgcact gttttttatg ccgcatactt cttggatttt cgcaaccctc gcaatcgcca
     1921 tattggtgtt gctccgccat aagagcaaca tcctcaacct gattaaaggc aaagaaagca
     1981 aaatcggtga aaaacgctga ttcggcattt gttttccatt acgtcatgcc gtctgaaacc
     2041 atgttcccgc gttcagacag catttccgca caaacggctt tcataccatg ctcagttaca
     2101 gacacgcatt ccacgccggc aaccacgccg atatgctcaa acactttacc ttgtttttag
     2161 tgctgcaata tttcaaccgc aaagacaagc cctactggta catcgacacg cacggcggcg
     2221 cgggtgtgta caatctcgaa ggcagcgagg cgcagaaagt cggcgaatac cggcaaggca
     2281 tcgccctgct ccgacaggcg caaaacctgc ctgccgaact ctctgacttt gccgcgcaca
     2341 tacaaaaaat cctgccctcg cccgaacttt actgcggttc tccgtggctg gcacaatcgc
     2401 tgacccgcgt cggcgacaaa ttacgcctgt ttgagctgca ccccaccgac tttgtccatc
     2461 tgcaaaacaa tatgggcgaa gcaggattgg gcaaacgcgg acaagtgttg cgcgaagacg
     2521 gttacaaagg gctgatttcc ctgctgccgc ccccgccgcg ccgtgccgcc gtcctcatcg
     2581 acccgcctta cgaggaaaaa caggactact ggcgtgtaac ggaaacgctg aaatccgctt
     2641 taaaacggtt tgagtccggc tgttatctca tttggtatcc ctgcctcagc cgcgaagaaa
     2701 gccgcaagct gcccgaagaa ctgaaaaaac tcgttcccga caactacctt cacgccgaac
     2761 tgcacgtcca cgcgccgaaa gccgacggtt tcggtatgca cggcagcgga atgttcgtca
     2821 tcaatccgcc ctatctgttg gcggaacaat tggcggcaaa cctccccgcc ctgacgcggc
     2881 tgctggcgca agacgaaggc gcgcgctacc ttttggacag caaaatccgc tgaccccgat
     2941 gccgtctgaa acccttcaga caacatctcc cgtcaaataa acaaaaccgg acgatatacc
     3001 ggcagtcggc acttttaaga ataaatatcc cctcatccgc ctgccgcgtt ccgacttgcc
     3061 ccatgccgtt tttttacact acaatcccct agactatcct gcaaaacgca ccaaaggaat
     3121 acgccacaat aacctgccgc ctttcagacg gcacaaacct ttccgaaacg agcaaactat
     3181 ggacacactg acccgattcc tccccgaaca cctgcaacaa aaccagctgc ccgaagcact
     3241 cggcggcgtt ttactgtccg ttgtttccgc ctgcaccgaa atcaacgcca aagtccgcct
     3301 cggcgcactg gccggcgtat tgggtatggc gggcacgggc aatattcagg gcgaagacca
     3361 aaaaaaactg gatgttatcg ccaacaacat catgattgac acactcaaag ccaaccctgc
     3421 cgttgccggt ttggcaagcg aggaagaaga ctctttcgta agtgccggtg aaaacggacg
     3481 ctatctcgtc ctattcgacc ctttagacgg atcgtccaat attgatgtca acatttccgt
     3541 cggtacaatt ttctccatcc ttgaaaaacc cgaaggcgca ttggcaaccg aatcattcct
     3601 tcaaacgggc agacagcagc ttgccgcagg ctatatcctc tacgggcctc aaacccagct
     3661 cgtattcaca ttcggacacg gcgtatatat gttcacgctc aatgccgaaa acgaatttgt
     3721 gctgaccaaa gaaaaaccga aagtacccga aagtaccaaa gaatttgcca tcaatatgtc
     3781 caaccgccgc cactggctgc cccccgtcca acaatacatc gacgagctct tggcgggcga
     3841 aaccggcacg cgcggcaaaa actacaatat gcgctgggta gccagcatgg ttgccgaaat
     3901 ccaccgcatc ctgatgcgcg gcggcgtgtt catgtatccg caagacaaac gcgacccgtc
     3961 caaacccggc aaactgcgcc tgatgtacga agccaaccct atgagcctga tactcgaaca
     4021 ggcgggcgca tcggcaagca acgcatatca agccatgctc gacatacagc ccgaaaacct
     4081 gcaccaacgc gtcgccgtct tcatgggcag cagcgaagaa gtcgcctatc tcgaccgcct
     4141 gcacgcaaaa tagacagaca gccgttcaca caagtacacg gcggcggcaa tccgataccg
     4201 ccgccgttat ttcaaacagc acaagccttc tggtcaaatg acaaaaatgc cgtctgaaaa
     4261 catttcagac ggcatttcaa aaactcaaac ccgaattatt ttcccgctgc tttttgcagg
     4321 atggcgtaca actcgtcttt cagtttcaat ttggcttttt tcagctcatc gatggtttcc
     4381 gcaccgctgg taaccggatt gttgaccaga ccggtaattt tatcgtccag ctcgttgtgt
     4441 tcgtcgaaca gacgggcgaa gcgggaattt tcctgtttca atttggaaat caaatcacgg
     4501 tattctggga acatatcgct ttcctctctt ggtttacggt taaaaagtca aactgaatct
     4561 ttacattaat tataacaaag ataatatttt tatccagtgt tttatcggat acggcaaaat
     4621 tttcttttaa caccgccgtc cggttgataa aaacgttgac aatatcacgc tttacgcata
     4681 aattttcagc ctttccta
//
LOCUS       DAXHKX010000153         4648 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHKX010000153 DAXHKX010000000
VERSION     DAXHKX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.153"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            108..1049
                     /gene="miaA"
                     /locus_tag="ACFMWZ_001755"
     CDS             108..1049
                     /gene="miaA"
                     /locus_tag="ACFMWZ_001755"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC5935681.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVTDPFSDGNSCTRLIEAAKRFFGA"
     gene            1053..1772
                     /gene="tadA"
                     /locus_tag="ACFMWZ_001756"
     CDS             1053..1772
                     /gene="tadA"
                     /locus_tag="ACFMWZ_001756"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC5935682.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGAVQTFLLLKASGLT
                     LTKSTLWQLQSLLDGTPPQEMAQHDKTRLLAELKNHPPVAAFPPQGEMAHFMGLALEQ
                     AKLSALMGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1777..2265
                     /locus_tag="ACFMWZ_001757"
     CDS             1777..2265
                     /locus_tag="ACFMWZ_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC5935683.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2332..3084
                     /gene="rlmB"
                     /locus_tag="ACFMWZ_001758"
     CDS             2332..3084
                     /gene="rlmB"
                     /locus_tag="ACFMWZ_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC5935684.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3146..4537)
                     /locus_tag="ACFMWZ_001759"
     CDS             complement(3146..4537)
                     /locus_tag="ACFMWZ_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC5935685.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
BASE COUNT         1154 a         1503 c         1122 g          869 t
ORIGIN      
        1 ggcatacggt aaacacgccc ccttgccgtc ggcgtgcttc cgcaaaccaa aatataacgt
       61 cggcacgctt tccgctataa tccgcatttt cgcaaccctg aaacatcatg ccgaccccga
      121 aagcctttgc cctgctcggg ccgaccgccg gaggcaaaac cgcgctcgcc ctcaaaattg
      181 ccgaaaccct gcctgtcgaa atcatcagcc tcgattccgc gctggtctat accggtatgg
      241 acatcggcac ggcgaaaccg tccgcctccg aacgcgcctt tgtcccgcac caccttatcg
      301 acatcattac gcctgttcag acatacagtg ccgcccgttt tgtcgaagac tgcacgcgct
      361 tggtcgggga aatttcctca cgcggcaggt ttgcccttat tgtcggcggc acaatgatgt
      421 atttccgcgc cctgacccaa ggtttgaacg atttgcccga agccgatgcc tgcctgcgtg
      481 ccgacttgga cgaacaaaaa caaatgtacg gcttggattt cctctaccgc accctgcaac
      541 aggtcgaccc tgaaacagca tgccgtctga aaccgaacga cagccagcgt atcggacgcg
      601 ctttggaagt ttattattta accggcaaac cgatgagtac gcatctcggc agcccaacct
      661 cccatacgct ccccttcgat ttgcataccg ccgccctgat tcccgaaaac cgcgcccgcc
      721 tgcatgaaaa catcgccctg cgtttccacc tgatgcttga acagggcttt atcggcgaag
      781 tagaaaacct gcgccgcctc tatcccagcc tgaccgccga ctcccctgcc atacgctgcg
      841 tcggctaccg tcaggcatgg gaatatctgg acggaaaaac cgatttcccg gcatttgtcg
      901 agaaaggcat tgccgccacg cgccaacttg ccaaacgcca actgacttgg ttgcgcaaaa
      961 cacctttaga ctgcgttacc gacccgtttt cagacggcaa ttcatgcaca cgcctgattg
     1021 aggcggcaaa acggtttttc ggcgcataaa ctatgctgac cacgccgccg ctcgccccca
     1081 aaaccgtcgc cgccctacac aggctcggta tccgtacgct cgaagaactg cggcaaaacg
     1141 gcgcggttca aaccttcttg ttattgaagg catcaggttt gaccctcacg aaaagcacgc
     1201 tgtggcagct tcagtcgctg ctggacggca cgccgccgca agaaatggcg caacacgata
     1261 aaacccgcct gcttgccgag ttgaaaaacc atccgcccgt cgccgccttt cccccgcaag
     1321 gcgaaatggc gcatttcatg ggtttggcat tggaacaggc gaaactgtcc gcattaatgg
     1381 gcgaaatacc cgtcggggca gtcatcgttt cagacggcaa aatcatcgca tcggcacaca
     1441 acacctgcat tgccgactgc aatgtcagcc ggcacgccga aatcaacgcc ttggcacagg
     1501 caggcagcga aatgcaaaac taccgccttg acggatgcga catctacatc accctcgaac
     1561 catgcgccat gtgcgcgtcc gcactgatac aggcacgaat caggcgcgtg atctacggtg
     1621 cggcagaacc caaaaccggc gcggcaggaa gcatcgtcaa cctgtttgcc gacaaacgcc
     1681 tcaatacgca caccgccata cggggcggaa tcctgcaaga agaatgccgc gccgtattaa
     1741 gccgtttttt ccaaaacaaa agaaaaggtt gaacctatga ccgccttgct cgtcatcctc
     1801 gccctcgccc tgatagccgc cggtacggcg ggcatcgttt accccgccct gcccggattg
     1861 gcattgatgt ttgccggaac ctggctgctt gcctatgccg gcggctacca aatctacggc
     1921 gcgggcgttt tgtggacggt cggactcatc agccttgccg gcatactggc ggactatgtg
     1981 gcaggcatat gggggacaaa atataccgga gcgggcaagc tcgccgttcg cggcgcattg
     2041 gccggcagca tcatcggcat atttttctcc cttcccggac taatactcgg tccctttatc
     2101 ggcgcggcgg caggcgaact gatcgaacgg cgcaatatgc ttcaggcagg taaagcgggc
     2161 ttgggtacgc tgttggggct tgtcgtcggc acggcgttca aaatcggctg cgccgtatcc
     2221 atcttgttta tcctgttggt gaaatacatc gcctacctgt tttaaccctt tcagacggca
     2281 taccggcaaa ttgctataat gctgtctgaa ccctttttac aaagcaccat catggcaaac
     2341 caaagaccca tctacggctt ccacgccgtc aacgcccgat tgtggcaaaa ccccaaatcc
     2401 atcgtcgaac tctacatcca agaaggcaaa tcagacgcac gcacgcgcga agtgttggaa
     2461 aaggcggcaa acgaaaacat ccgcgtatat tttgtcgatg ccgaccgcct caacgccatc
     2521 agcaaaggcg cgcgccatca gggcgtggtc ggatttatcg atgcctccaa aaaccacgtc
     2581 cacctcgaag acgtattgga aaacctcagc gaaccgccgc tgctgctgat actcgacggc
     2641 atcaccgatc cgcacaacct cggcgcgtgc ctgcgtaccg ccgacgcaat gggcgtacac
     2701 gccgtcatcg caccgaaaga caaaagcgcg gggctgaacg ccaccgtcag caaagtcgcc
     2761 tgcggtgcgg cggaaaccgt cccctatatc accgtaacca acctcgcccg caccctgcgc
     2821 gaattgaaag aatacggcat ttggattatc ggcaccgata tgagcggcga gtccgacctt
     2881 taccattgcg atctgcccga cagcgcggcg tgggtaatgg gcaacgaagg cgacggtatg
     2941 cgccgcctca cgcgcgaaca ttgcgacatg ctggtgtcca tacccatgtt cggcacggtc
     3001 gaaagcatga acgtctccgt gtctgccgga atggtattaa gcgaaacccg ccggcagcgc
     3061 gtattaaaaa acgaaaaagc gtaagcctca aaaacaatgc cgtctgaaac agggattctg
     3121 tttcagacgg cattcatttt caaaatcagt gttccaagtg gtcggtatca aacttgaccg
     3181 ccgcctcggt tttatcttcg ggcaagacca aattcagcaa gactgccgta atgccgccgg
     3241 cggaaataga gttttggaac aagacgggca ggtttttaaa cacttccggc tcaaacgcga
     3301 cacccaagcc caagccgacc gaagtcgccg caatcaccgc ctcgcgcctg cggatgccgt
     3361 ggctgaccaa aatccgcaca cccgcaatcg caatcaagcc gaacatcaaa accatcgcgc
     3421 cgcctaaaac cggactcgga atcgtcgtaa acgcgcgtcc gacaacgggg aacagaccca
     3481 acagcaccaa aatcacggca atatatttgc ccacatggcg cgaagccacg ccggtcatct
     3541 gaatcacgcc gttgttttgc gcaaacgttg tcagcggcag cgaacccaaa gccgtcgcaa
     3601 tcaccgacac caagccgtct gccaacacgc cgccgcgcag gcgtttggtg tattcctcgc
     3661 cttcaatcgg ctgatcggac accattgccg ttgcggtcaa atcgccgacc gcctcaaaca
     3721 cgctcaacag gaaaatcgca cccgccacaa taaatgcgtg ccagtcaaaa gcaaaaccat
     3781 atttaaacgg tacgggcagc gtaaccagcg gcaggttttg cagtgccgaa aaatccacct
     3841 tgcccaaaaa cagcgcgacg atatagccgg caatcagacc gaccgcaatg ccgctcatcc
     3901 gcagcagcgg gtttttcatg cagttgaaca ccagcacaat cagcagcacc agcgatgcca
     3961 gccccaagtt ttccatcgag ccgaacgtgc cgtccgcctt tgcgccgaag ccgccgccga
     4021 aatcggtaat accgacgtgt accaaactca agccgatcag catcaccacc acaccgctga
     4081 ccgtcggcgt aatcactttt ttcaaatacg gcagaagcca cgccgaaaaa cacaccaaaa
     4141 acgcgccgac aaacgatacg cccaagagcg tcgaaatcat cgcatcctta gtcaaaccgc
     4201 cctctttcat ccccgcgccc agcgcaatca tgacggtaac gaacgagaaa ttcaccgact
     4261 ggatagacag catccccgaa ccgaccggcc cgaagcggtt gacctgcaaa taagtgccga
     4321 cacccgacgc aaccatcgcc atcgacacga gatacgccgt catctccacc ggcagttcca
     4381 gcgcgccgcc cacaatcagc gcgggcgtaa tcatcggcac gaaaatcgcc aaaagatggg
     4441 taaccgcgct caacagcgca ttaccgaacg gcggcctatc ctccaaaccg tacaccaaat
     4501 caggcgcatc cgcccctttg cccaactgac cgctcattgt atttccttat tttaaaggtt
     4561 gaaaaatcca agcgcggatt gtagcgaatc tgccgcacgg cttcaattta catttcagac
     4621 ggcatccgtc tgccccacac gtttacca
//
LOCUS       DAXHKX010000154         4611 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHKX010000154 DAXHKX010000000
VERSION     DAXHKX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.154"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(67..291)
                     /locus_tag="ACFMWZ_001760"
     CDS             complement(67..291)
                     /locus_tag="ACFMWZ_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC5935686.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="ACFMWZ_001761"
     CDS             complement(292..1680)
                     /locus_tag="ACFMWZ_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC5935687.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="ACFMWZ_001762"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="ACFMWZ_001762"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC5935688.1"
                     /translation="MTFDEWLGLSKLPKNEARMLLQYASEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYLLGWREFYGRRFAVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPSLETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="ACFMWZ_001763"
     CDS             complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="ACFMWZ_001763"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC5935689.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT         1031 a         1408 c         1235 g          937 t
ORIGIN      
        1 ttaggtttgc atcggcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agctctgtga tgcggtgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatattg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgttgtcc gtcggcgttc agccccatcg tcgggcccaa
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacgatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccaacc atcgccatca tcttgatgcc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttgatgacg cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcggcggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc cattacgttg atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatatagg tcgtaaccag cccgaaagta atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagaggc ggaacaatca tcgggataaa
     1261 ggcaatgtgg atggggatga tgttctgact catcatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagcttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 tgcaaacgcg ccgagcatcg cataagaaag cgcaatcttc gcaccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctggct gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcaccagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 gaaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaag tccggcaggg tttccactcc cgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtgcggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcgacaaa tgtttatcac
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatcc gccgcatttt
     2161 tccgcgccgt ttcaagggag ggcgggctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagcgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttccgg caggcgcgcc aatacggctt cgaccaaatg ttcggtttcg gggcgcggaa
     2341 tcagcacatt cgggttgacg gcgaaacgcc gtccgtaaaa ctcgcgccag cccaacagat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttcaccg ccccgcgtca acaactgcac gcgcgtatat tccgaagcat
     2521 attgcagcag cattcttgct tcatttttgg gcagttttga caagcccaac cattcgtcaa
     2581 acgtcatttt tatcccgtct gccgctgatg cggcttttct ttccttattc tttccggcaa
     2641 acgtaccgat ggtggcaacc gcaaatgcgg cataccacaa ataaaatcct gcaccgtagc
     2701 gcacaatatc cgatgtattc cctgcttcat cgacgtatac ggctttcaca ctgaaagcca
     2761 ccaacgccaa gccccaaagt gccgcatgga caggcacgac cttcttccgc aacgccagca
     2821 aaacaatggc cgccaaccaa acataattcg catagaccgc acaatacctg atatccaaag
     2881 aagcaaatat cgaccccaaa atcaaaacgg tcaaaccctc catgcttcca tgattgccca
     2941 aataaaatgc aacattggat aaagacgcta tccacagggc aaccgacacc agcaacatca
     3001 ctatgggaaa acttgatttc cgattctgtt cctgcatggt tttatcctaa tgtaaaaggc
     3061 cgcctgaaaa cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg
     3121 acggtcagcc cggcatcgat acgcagggtc ggctgcccca cgccgacggg gacgctctgc
     3181 ccttctttgc cgcacacgcc gacaccgctg tccaacgcgg tatcgttgcc tatcatcgaa
     3241 acgtgtttca gcacttcggg cccgttgccg ataatggtcg cgcctttgac ggggtattgc
     3301 agcctgccgc cttccaccca ccatgcttcg gacgcgctga acacaaattt gccgctggta
     3361 atgtccacct gtccgccgcc aaagttgacg gcgtaaatgc ctttgtcgat ggacgcgatg
     3421 atttcttccg gctcatagct gccattttcc ataaaggtat tggtcatgcg cggcatcggg
     3481 acggaggcgt agctttcgcg gcggccgttg ccggtggatt gcgtacccgt caggcgggca
     3541 ttggtttcgt cctgcatata gccgaccaaa ataccgtctt caatcaatac ggtgcggcgg
     3601 gtttcgttgc cttcgtcgtc gatgttgagc gaaccgcgcc ggccggcaat atcgccctga
     3661 tcgacgacgg taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg
     3721 gtccccttgc ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg
     3781 cccggccagc cgttgcccaa aacgacggtc atttcgccgg cgggcgcggg gcgggattcg
     3841 aggttggtga gtgcctgttt gacggcggca tcgacaaact gccgcaccaa gttttcatcg
     3901 aaataagcca agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc
     3961 tgtttggcga tgacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg
     4021 ccgtccagac gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc
     4081 acgatgcgcg gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg
     4141 gcggcggaat cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt
     4201 tcagacggca ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcgg
     4261 gcggaacggt ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg
     4321 cccgaaacgg cgcgcacgcc cacgccctga ttgatttgga agctgcccga tttgacgatg
     4381 ccctcttcca aatgccagct ttcataagcg gtgcgctggc agtagatgtc ggcgtaatcg
     4441 acgtggtgcg cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg
     4501 gcttcgagca gccgcgcctg tacggcggaa taggtcggat gcatagtgtc ggcgcataaa
     4561 aaatcagggg cttgattata cggcatttgt tatatagtgg attaacaaaa a
//
LOCUS       DAXHKX010000155         4578 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHKX010000155 DAXHKX010000000
VERSION     DAXHKX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.155"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(27..248)
                     /locus_tag="ACFMWZ_001764"
                     /pseudo
     CDS             complement(27..248)
                     /locus_tag="ACFMWZ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase TauD"
     gene            complement(247..354)
                     /locus_tag="ACFMWZ_001765"
                     /pseudo
     CDS             complement(247..354)
                     /locus_tag="ACFMWZ_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(400..1128)
                     /gene="pdxJ"
                     /locus_tag="ACFMWZ_001766"
     CDS             complement(400..1128)
                     /gene="pdxJ"
                     /locus_tag="ACFMWZ_001766"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC5935690.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1152..1943)
                     /gene="recO"
                     /locus_tag="ACFMWZ_001767"
     CDS             complement(1152..1943)
                     /gene="recO"
                     /locus_tag="ACFMWZ_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC5935691.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(1977..3104)
                     /gene="pheA"
                     /locus_tag="ACFMWZ_001768"
     CDS             complement(1977..3104)
                     /gene="pheA"
                     /locus_tag="ACFMWZ_001768"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC5935692.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3143..4375)
                     /locus_tag="ACFMWZ_001769"
     CDS             complement(3143..4375)
                     /locus_tag="ACFMWZ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC5935693.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
BASE COUNT         1022 a         1321 c         1254 g          981 t
ORIGIN      
        1 cactatatta cataatcatc gcctcaatat ttaatatccc aaaatataga tttaaaacca
       61 tcgcctttgt ggtttgtcag cattcctaat tgagatagaa agtcaaataa tttcttgttt
      121 ctccggctgc catcaagatg gtttagatta agacctcgaa caatacaata cccaatactc
      181 tgtactgtat tgtgaatttc ttctatcaaa ctattattgt ttgcagataa aatatacttt
      241 tgattcatat agtgaattaa atttaaacca gtacggcgtt ggcttgcctt gccgtactat
      301 ttgtactgtc tgcggcttcg ccgccttgtc ctgatttaaa tttaatccac tataacatat
      361 ccctgtcgtg aaatgctgtc tgaaagggtt tgcctgccct tacggcagca gccttgccct
      421 gaacatcgcc tccttcattt ggcgcacggc ttcgggcagt ccgaggaaga gggcttgggc
      481 aatcagcgaa tgcccgatgt tcagttcgcg gatggcgagg atttgggcga tgggggtaac
      541 gttgtgtatg gtcagtccgt gtccggcgtt gacgaccaag cccaaatcgc cggcaaaatg
      601 cgcgccgttt tgcagacgct cgaattgctt gagttgttcg gcgtggctgc gcgcgtcggc
      661 atacgcgcct gtgtgcagct cgacaacggg cgcgccgaca tcataggcgg cttggatttg
      721 cctgtcgtcg gcatcgataa agagagagac tcggatgcct gcgtcggtca ggattttggt
      781 gaacccggcg attttttcct gttgcgccaa tacgtccaag ccgccttcgg tcgtgatttc
      841 ctgccgtttt tcaggcacga tgcacacgtc ttccggcatc actttaagcg cgttttcgag
      901 catttcttcc gtcaacgcca tttcaaggtt caggcgcgtg cggatggcgt ttttgacggc
      961 aaacacatcc gcgtctttga tgtggcggcg gtcttcgcgc aggtgcatgg taatcaaatc
     1021 cgcaccgtgc gtttcggcaa ccagtgccgc ctccacgggg ctgggataag tcgtgccgcg
     1081 cgcattgcgg acggtagcga tgtggtcgat gttgacacct aaaagcataa tctttccttt
     1141 tatttctgcc ttcagacggc atttgaagcc gtgccgtccg aagtcgggac ggtttcccgg
     1201 gcggtttctt tgcggtcaaa ctgccgtatc tgttccaaca cctgccgcga tttcagcccc
     1261 tcgggcaaca ggtggcggat aaaaagccgt gtgattttca atgcctgttg caggctttcg
     1321 gcggtgcgga aactgccttc gcgcaaatcg atcaggcttt gcccttcgac aattgcgccg
     1381 gcttcgtgcg aaagcgcgtc tgcgtcctcg catacgggca tgaccgcctc ttcgggcatc
     1441 agccggtagg ttttgtccgc caaaatatcg ccgcccgtgc cgtcggcgtg caaatcgggg
     1501 gcaacgccca acaggttcag cagccgccac tcgaaacggc gcaagtcgtc gatataagcg
     1561 gctttgcagc acaccgcctc catcacttcc gccaacgcgt cgtataactc gggcaacgga
     1621 tcttcgcgga cggtcaactt cagcatcaac tcgttcacat acaatccgct gaacaaagcc
     1681 ctaccctgcg gctgcggcca cccgcctatc cattcggcgc gatgtagggt cttcaactct
     1741 tgcgaaccat accaagacgc actcaccggc acaaacggca ccaacacgcc gcgcaattcg
     1801 ctctgccgtt tgcgcgcgct tcttgccaaa agtgccacac gcccgtaacg gcggctgaac
     1861 gcttcgaccc gcaggctgct ttcgcgccag ggcgaggaag ccaacaggaa gacgggttcg
     1921 tggttgactc ggtgttcgga catcatgcct gaaatgccgt ctgaacgctg ccgccgctac
     1981 aaaacggcgg tcgggtacga accgatgact ttgacgaacg aagcgcgttc gcccaagcgt
     2041 tccaatgccg tctgaatctg cgcgtcccgg cggtgtcctt cgatgtcgat gaagaacagg
     2101 tattcccaca aaacggattt actcggacgg ctctcaaact tggtcatgga aatacccgac
     2161 tcagtcagcg gttgcagcag cgaggcaacc gcgcctgccc ggttgggcgc ggaaacggcc
     2221 agcgaagtct tgtcgctgcc gcttgcaccg gtttcgtgat gtcccatcac caagaagcgc
     2281 gtggtgttgt tcggttcgtc ttcgatgcac tcggcaacca tatcgagtcc gtagatttcc
     2341 gccgccgtgc gtccggcgat ggcggcaacc gtaccgtcgt ccgattcggc aaccagcctt
     2401 gcggcttcgg cattgctgga cacggcaatc cgttcggcgt tgggcaggtg tctgcccaac
     2461 cagtcgttgc actgcgccaa cgcctgcgcg tgggaaaaga ctttggcaat gccttcggtg
     2521 ctgccgttgt ttttacgcaa aaggttgtgg tggatgcgca aaacgacttc gccgcacgcc
     2581 tgcaacgcgg taacggcaag caaatccaac gtgcgcccga ccgagccttc ggtcgagttt
     2641 tccacgggcg cgaccagata atccgcctga cgggtttcaa cctgcttgaa gcagttgtct
     2701 atggtcggac acgccatggt gtgcgcggcg tgtccgaaat gcttgatcgc cgcctgctgg
     2761 gtaaacgtgc cctgcggccc cagataggcg atggtcagcg ggcgttcgac ggcgaggcac
     2821 tcgctcatca cttcccgaaa caggcgtgct accgattcgt cgggcagcgg gcctttgttc
     2881 aaatcctgaa tgcggcgcaa cacggcgact tcgcgttcgg ggcggtacac tgcgcccgtg
     2941 cctttcagct cgccgatggc gtgggcgtgt tgcgcacgtt cgttgagcag gcgcaggatt
     3001 tcggcatcga tggtgtcgat ggcgttgcgg tggggaagga ggagttcgtc gatagtttgg
     3061 gacataattt cgcctgaaca gtaatttatc gtgcattcta acatcaaatg ccgtctgaaa
     3121 cggtttcaga cggcattttc cgttaaaggt attcgctctg ttcgttttcc ttccacgccc
     3181 ggtgcgaaca cagccacaga agcgcaatgc cgcaagaggt ggacgcggtc atcgttgccg
     3241 ccatcacggt tgccgaaccg tcgtgcaaga aggtcgccgc catacccacc cccgcgccga
     3301 ttaaggactg gaacacgccc aacacggcgt tcgcgctgcc gccctcttct ttgaaataag
     3361 acataaagca tgcctgcgtg ttcgcgccga ccaagccctg cgtgccgacg gaaaacatca
     3421 cgcacgccac caagagccag aacggaggca acccgaaaaa cagcacggcg gcgagttggg
     3481 acaggttggc ggcaaattgg acgacaatcc cccacagcag gatgctttgc ggatgcgcgc
     3541 cggttttgag ccgccacgcg gtaatgcggt tgaaaaacat catcgtgatg atgttgagtg
     3601 caaacgccca agcgtattga tgaggcgtaa cacggtaaag ctgctggtac acgaaggaag
     3661 attcggtcag aaaggcgaac atcgaaccga agctgaatgc ctgaaaaaac agataaccca
     3721 tcgcggcacg ggttttcaat acgcgtttga accgcccagc caccagcccg aacacatccc
     3781 gtccgatttt gccgccgacg gcaggcttgg gcaggaaata ctgcaccaaa ccgagcagca
     3841 ccagcgaata cgccgccaga aaaacaaaaa tcgcctgcca gccacccaag ccctgcaaca
     3901 gtgcgccgac catgggtgcg accagcggca caaccatcaa aatgatgccg ataagggcaa
     3961 acatctgggc ggctttgcgt ccggaataat aatcgcgcac cattgcgccg acgatgacca
     4021 cagtcatgcc cgcaccgaat gcctgcacga cgcgcaggtt gaggagctgt tcggcactcg
     4081 aagcaaatac gatggcggca acggcaaggc agtagacggc cagtccggtc agcgcgacgg
     4141 gtttgcgccc tttgatgtcg gacaccgaac cgccgaccac ctgtccgaac gccgtgccga
     4201 acataaacaa actcaggctc tgttcgatgc ggtggacatc cgcgttcagc gactgcgcca
     4261 tttcgggaat cgcgggcagg taggcatcga tggaaaacgg catcagcgta accagcatcg
     4321 ccatcaaaac cgccatcagt ttttcgctca tttcaggata atgggcagaa ggcataaatt
     4381 ctcgtccgat tcgcaaacaa accgcccggc ggcggaaaac acaatacgcg gaaacggaaa
     4441 attttaacac accggcacaa acaggcggca acaaaagccc cggcaaaacc ggacgaaccg
     4501 gaacgatata gtggattaac aaaaaccagt acagcgttgc cttgccttag ctcaaagaga
     4561 acgattctct aaggtgct
//
LOCUS       DAXHKX010000156         4558 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHKX010000156 DAXHKX010000000
VERSION     DAXHKX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.156"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(150..1193)
                     /gene="argC"
                     /locus_tag="ACFMWZ_001770"
     CDS             complement(150..1193)
                     /gene="argC"
                     /locus_tag="ACFMWZ_001770"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC5935694.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIWDIPTWEHWYGMPHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1190..1471)
                     /locus_tag="ACFMWZ_001771"
     CDS             complement(1190..1471)
                     /locus_tag="ACFMWZ_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935695.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            1725..2880
                     /locus_tag="ACFMWZ_001772"
                     /pseudo
     CDS             1725..2880
                     /locus_tag="ACFMWZ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            2889..3095
                     /locus_tag="ACFMWZ_001773"
     CDS             2889..3095
                     /locus_tag="ACFMWZ_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935696.1"
                     /translation="MKNWKQFTFFVILVIACYQLLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNANQ"
     gene            3300..3659
                     /locus_tag="ACFMWZ_001774"
     CDS             3300..3659
                     /locus_tag="ACFMWZ_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC5935697.1"
                     /translation="MERKNNIIELSIEDLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGAGIVSGGIPAGPAIILGGTVGAAVGAATGMGVAAYEYYQETQPKGTVTVGPISA
                     FAVDKSGNGYTDSTNYC"
     gene            3720..4346
                     /locus_tag="ACFMWZ_001775"
     CDS             3720..4346
                     /locus_tag="ACFMWZ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HFC5935698.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTAEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGDSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            4250..4498
                     /locus_tag="ACFMWZ_001776"
                     /pseudo
     CDS             4250..4498
                     /locus_tag="ACFMWZ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFP transporter"
BASE COUNT         1207 a         1005 c         1178 g         1168 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacgggattt taggtttttg ttttcgtggg aatggatggg
       61 cccaaagccg tccgaaccaa aaaaaaccgc cgctttcaga cggcatcaaa aaaccgcagg
      121 cacgatgcct gcggtttgcc gtttgcgctt caggggagca gggggattgc gcccaagccg
      181 tgtgtttctt tcagtccgaa catgatgttc atattttgca ccgcctgacc cgccgcgcct
      241 ttgacgaggt tgtcgatgac ggaaaggacg acccacacat cggattgcgc cgcctgttgg
      301 atgctgatgc ggcagaggtt tgcgccgcgc acgctgcggg tttctggtgt cgaaccggcg
      361 ggcaggatgt ccacgaacag gctgtcgcgg taatagtcgc gcaggatggt ttcggggcag
      421 atgccgtctg aaaggtggag gtaaacggtg gcgtgcatac cgcgtatcat cggcgcgagg
      481 tgcggcgtga acacgaatcc ttcggcgatg ccgtcctgaa gcccggcgat ggtctgcctg
      541 atttcgggca ggtggcggtg tccggctatg ccgtaggctt tgaagttgtc gccgacttcg
      601 cacaacagcg aaccgacatt gcctttcctg cccgcgccgg acacgccgga tttgcagtcg
      661 gcaatcagcg gcataccggg cttcagacgg cattgccgca acagcggcac gagcggcagg
      721 gatacgcagg tcgggtagca gccggggttg gcgacgaggc gcgcctgtgc gacggcttcg
      781 cggttgagtt cgctcaatcc gtacacggct tgggaaacga ggccgggggc ggcgtggggc
      841 atgccgtacc agtgttccca ggtcggaatg tcccatatgc ggaagtcggc ggaaaggtcg
      901 atgacgcgca cgccctgttc aatcaggcgc ggcgcgtctt tcatggcgat gccgttgggc
      961 gtggcgaaga agacgatgtc gcattgttcc aaacctgcct cgtcgggcgt ttggaaggcg
     1021 aggccgtaca cgccgcgcaa actcggaaag taatcggcaa ctgcggttcc cgcttcgctg
     1081 cggctggtta cggcggcgac ttcgacatcg ggatgggcgg caagcaggcg cagcagttcc
     1141 acgccagtgt agcccgtcgc gccgacaatg ccgactttga tttttttact catggttttt
     1201 cctttgtgtg gttggcgggt atgccgtccg aacgctgtcc gacggggttt tgattggcgg
     1261 cggaaggacg gtacacaaag cgtttgaaaa gcgtgtctaa tttaatggca tccgcctctg
     1321 tttttaaaac ccagccctgc cgtccggaat aaacataacc gtaggcgcga aagccggcag
     1381 gatatttcgg tgcctgcccc aaacccaaac cgtatgcggc gaaagcggtg gtcgtggaga
     1441 aggaaagcag gaggaggacg agggttttca taaggttcca aaccgaacgg ggcgatgaag
     1501 cccgattata gcaaaaggtc gtctgaaaac cgttttcaga cgacctttta tttaattaca
     1561 ataaaaatgt taacaacaaa aaacaaaccg cttttttccg tttggagaca aatttttaac
     1621 taaaattgtc aacaattcct tgacgcacac acaaaaaaaa ctttaatatt tgtaaatata
     1681 tgtaacatta taaattttaa atattttctg agtaagggga agtaatgagc atccatctcg
     1741 acttcggcat tagtcctaaa acgttccgac agacttaatc tgtatcaaaa gcccaagctc
     1801 tttaaaggag cggttcggaa tctcgaagcc gcatcttgga aagatatcaa cgagatatac
     1861 caacgagcag acccaaccgc accgctgttt catctgcgta aaaaaggcgc aatcgttcct
     1921 aaagaagaat acgtcgaaag tttcgacgat ttgggcaaaa ctcgctaccg ttttattaaa
     1981 tccgttatct acgaacatat gaagaatggt gcgtcgttag tctataacca tattaacaac
     2041 gagccgtttt cagaccatat cgctcgtcaa gtcgcccgct ttgccggcgc acatactatt
     2101 gttagtggat atcttgcttt tggcagcgac gaatcttata aaaaccattg ggacacccgc
     2161 gatgtgtatg ccatccagct tttcggcaag aaacgttggc aacttactgc ccctgatttc
     2221 cctatgccat tgtatatgca acagactaaa gatactgata tttccattcc tgaacatatc
     2281 gatatggata ttatccttga agcaggtgat gtcctctaca tcccgcgcgg ttggtggcac
     2341 agacctatcc cgctcggctg tgaaaccttc cacctcgctg tcggtacctt tccacccaac
     2401 ggctataatt acctcgagtg gctaatgaag aaattcccca cgatagaaag tctgcgccac
     2461 agtttctcag actgggagca agataggacg cgtatcaacg atactgccgc acaaattgct
     2521 gccatgattg ccgaccccgt caattacgaa gccttcagtg aagacttcct cggcaaagaa
     2581 cgcaccgata ccgcttttca tctcgaacag ttcgcgaatc ccaacgctac tccgctttca
     2641 gacgacgtca ggttgagact aaatgccaat aatttggata cgttggaaaa gggatatttg
     2701 attgggaatg ggatgaagat aagcgtagat gaattgggga aaaaagtttt agaacacatc
     2761 ggtaagaatg aaccgttatt gttgaaaaat ctactggtta acttcaatca gggaaaacat
     2821 gaagaagtta ggaagttgat ttatcagttg atagagttag attttctgga acttttgtga
     2881 gggattctat gaaaaactgg aagcaattta catttttcgt aatattggta atagcttgtt
     2941 atcaattgct atatttttta tcagatatgt ttcttctcga ctacattaac aaatatagtt
     3001 ggaatttgaa ttttattcag ggtactttga attttttttc aatatatctg ccatatgttt
     3061 ttgttagccg aatttttagg aatgccaacc aatgaaagac taggctatga aatcaaaatt
     3121 tgtgtataag ttactggtgc ttgcaaagta cctgtatatt ttaatttgta tatcaatacc
     3181 aatcgttttt atttatgaaa gctaccaagg agatgatgtt gattttatga tttatattcg
     3241 tgcttttatt attttttcca tattgggtgt attagctcaa tattctatgt ctaatttttt
     3301 tggaaaggaa aaataacatc attgaactat ccattgagga tctggagcta atttatggcg
     3361 gaggcttagg ggagagaatc cttgaaggag gagcaaatgg tgcacaaata ggaggatcaa
     3421 ttggtgcagg tattggtctg ggtgccggta tagtttctgg tggtattcct gccggaccgg
     3481 ctattattct cggtggcaca gttggtgctg ctgttggtgc tgcaactggt atgggtgttg
     3541 cagcatatga gtattaccaa gaaactcagc ccaaagggac agtaacggtc ggtccgattt
     3601 ctgcttttgc tgtggacaaa tcaggaaacg gctacactga cagcacaaat tactgctgat
     3661 atttctcatt gttcagacga tctctagggg tcgtctgaaa ctttcttaac ttcaatttta
     3721 tgaatagacc caagcaaccc ttcttccgtc ccgaagtcgc cgttgcccgc caaaccagcc
     3781 tgacgggtaa agtgattctg acacgaccgt tgtcattttc cctatggacg acatttgcat
     3841 cgatatctgc gttattgatt atcctgtttt tgatatttgg taactatacg cgaaagacaa
     3901 cagcggaggg acaaatttta cctgcatcgg gcgtaatcag ggtgtatgca ccggatacgg
     3961 ggacaattac agcgaaattc gtggaagatg gagaaaaggt taaggctggc gacaagctat
     4021 ttgcgctttc gacctcacgt ttcggcgcag gagatagcgt gcagcagcag ttgaaaacgg
     4081 aggcagtttt gaagaaaacg ttggcagaac aggaactggg tcgtctgaag ctgatacacg
     4141 ggaatgaaac gcgcagcctt aaagcaactg tcgaacgttt ggaaaaccag gaactccata
     4201 tttcgcaaca gatagacggt cagaaaaggc gcattagact tgcggaagat cagtggcaaa
     4261 aacggcactg ggcagacagg aattgtcggg attgggcatg gtatcctccg atttggcgaa
     4321 gagcaacgaa cctgtttatc tcgtgaaaat aaaacccgac aaaccaacca tcactgcata
     4381 cggtgaggaa aaaccgctgc aaatcggcat gacgttggaa gcagacatcc tacacgagaa
     4441 acggcgattg tacgaatggg tattggagct gatttatagt atgtcgggca aactgtaaga
     4501 cttgaggctg taaagataaa gaagttgtct gaaacctttt cagacgacca gaagacaa
//
LOCUS       DAXHKX010000157         4509 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHKX010000157 DAXHKX010000000
VERSION     DAXHKX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.157"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(127..1269)
                     /locus_tag="ACFMWZ_001777"
     CDS             complement(127..1269)
                     /locus_tag="ACFMWZ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC5935699.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WQVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1318..1740)
                     /locus_tag="ACFMWZ_001778"
     CDS             complement(1318..1740)
                     /locus_tag="ACFMWZ_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935700.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1733..2542)
                     /locus_tag="ACFMWZ_001779"
     CDS             complement(1733..2542)
                     /locus_tag="ACFMWZ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC5935701.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2624..3001)
                     /gene="acpS"
                     /locus_tag="ACFMWZ_001780"
     CDS             complement(2624..3001)
                     /gene="acpS"
                     /locus_tag="ACFMWZ_001780"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC5935702.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3201..3968)
                     /locus_tag="ACFMWZ_001781"
     CDS             complement(3201..3968)
                     /locus_tag="ACFMWZ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC5935703.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3978..>4509)
                     /locus_tag="ACFMWZ_001782"
     CDS             complement(3978..>4509)
                     /locus_tag="ACFMWZ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC5935704.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPTSFDKKQGVIWGNILSLNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1107 a         1128 c         1097 g         1177 t
ORIGIN      
        1 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
       61 aatatcggcc ggatattaaa aaaagccgtc tgaacatttt tcagacggct tttttgtaca
      121 ctttcctcaa cggttcgatc cctgcaccat atcaatcagg aacaggcggc agtcgaggtt
      181 gccgttataa agcggaattt tccgcttggg cgacaggcgc atgaatttgg gcatttccct
      241 gtcgccggta aacataccga cctgccagcc ggcatagtgt tttttcaacc acgtccccaa
      301 ctgcggatac agtgcctgca aagcgcggac ttcctcaagg cgcacaccgt agggcggatt
      361 ggacaccata atgccgccca cgccgttcgg acggacggat tgcgcgtcgg caacgctgaa
      421 ggaaacgatg tcgtccaccc cggcactgcg tgcgttggcc aatgccgtct gaatgatgcg
      481 gcggtcgctg tcgctgcccg aaatcggtgc aaaagccggt ttaacctgcg cttcggcacg
      541 gcggcgcaaa tccgaccaca gcgttttatc gaaattttgc agtttttcaa aaccgaaacg
      601 gcgcatcata cccggcgcgc ggcgggcggc aatccaagcg gcttcgatag caatcgtgcc
      661 gctgccgcaa aacgggtctt gaaacggctg cgtgccgtcg tagcctgccg agagcagcaa
      721 tccggcggca aggttttcgc gcagcggggc ttcgccggta tcgagacggt agccgcgttt
      781 gaacagggct tcgcccgaag tgtcgatgaa gatttcgaca ttgcgttcgt ccaaaaaggc
      841 gtggatgcga acatcgggcg cggctttgtc cacgctcgga cgtgcgtcgt aaatgtcgcg
      901 gaaagcgtcg cagacggcat ctttgacggt cagtccgaca aattggaggc ttttgacgtt
      961 ggcacgcttt gcctcgattt tgactttgaa cgtctgctgc aaggtaaacc agttgaacca
     1021 gttgatgttt ttggcaagtt tgtaaatgtc gcgctcggtt cggtatgtgc ctttggtcag
     1081 gcgtagcagg atgcggccgg cggtacgcga gtgcaggttg gcggcgtaaa cctgttccaa
     1141 tccgccccgg caggaaacgc cgccgtcaaa cacttgtaca tcggtacagc cgaggctttc
     1201 gagttcttga gataaaacgg tctccaagcc gcgcgggcag gtggcgaaaa gtgtatataa
     1261 agtattcata acgtgtcctt tccggacgga tgttccggca atggcggatt gaaaggttta
     1321 ttcggcggta aatgccgtct gaaatgtccg actgccgatt gtaatgtccg ccgtaaaatc
     1381 gcgcctgcct tcgacacaga tgggcaggcg gatgcgtact gcctgccaag tccccgacgg
     1441 ttgccgctcg aacatatagc ggttgaaacc catatccata tttttcatac tgaagctgat
     1501 gctgacctgt tccgtgccgg cgggcgcgtg ttcgatataa atatcaaacg gttttttggt
     1561 tgaaacggcg gcggcgcgga cgcggcttcc gtccggcagc gtgcaaccct cggtcaaatc
     1621 gcattgcgcc gccacagctt gcggctgctg cgcctgccac cattgcaaca aaacgagctt
     1681 gacggcggca aaggcaatca gcagcagtgc ggcaagcagt aatttacggt ttttattcat
     1741 gattcttccc tattgtttca tcccttacca ccacgtgcgc ccccaatccg agccatagtt
     1801 ttccataacg tgcaaccgtc tgtccgtttg ctgcaagtac gagcggcacg gatacgccct
     1861 gccgcagggc ttctgccgcc tcttgtgcct gaacatcatt gcacacgcgc cacacgacaa
     1921 catccgcgcc cttttcctgc gcctgccgcc attgttcgcg ggtctgcacc atcatccaca
     1981 cgctgtcggc gtgttcgggt ctgtcctgcc attgcgccgc ctccatcaaa acgttcgcac
     2041 cgcttccctc tgccgaaccc aaaggcagga cgcggtacgc gcccatactg ttctccccgt
     2101 gcaaacccgt tttcaggctg ccgtacaaac ggcgcggaac ggacagcgaa cgcaaaagcg
     2161 cgccgttggc gggcagcatg ggggcaacgg taaaatcacc cgccttctgc caagaccatt
     2221 cctgcccttc gcgggattgc ggttcgcccg tccattggcc ggggttgacc cataggaatt
     2281 tcaggcagac gcgggcgtgt tcgtaggaat ggattttggt cagccaaggc gtggcggcaa
     2341 ggatgcggat gccgagttct tcttcaaact cgcgttgcaa ggcttggaag tcggtttcgc
     2401 ccgcttcgac cttgccgccg gcaaattccc aatatccggc atagggtttg ccttcggggc
     2461 gcgagctgag caggtagttg ccgtctgaat cgagcaggat gccggcaacg acgcggataa
     2521 ggggtcgggt gtcttgaatc atggcgggta cgcggttggt cggtcggagt gcaaatgagg
     2581 gacgagcaat gccgtcaacg ggtttgccgc atttcagacg gcattatttt tcggcaacga
     2641 caaacgccaa tacggtgtct tcttcgtcgc tcatgctgag gctgacgcgg ctgatgcctt
     2701 gttcctccag ccatttggac agggcggggc cgtagaaaaa ttcgggcttg cccaatgcgt
     2761 catgcccgat gccgatgttg cggaaggaaa ccgcgccgcg tatgcccgtg ccgacggctt
     2821 tggcgaaggc ttctttggcg gcaaagcgtt tggcgaggta gttgacgggt ttgcccgctt
     2881 gcggaaattc aagcagctct tccggagtga ggatgcgccc ggcaaacgcc tgtccgaatt
     2941 ttttgtttaa gcggatgatg cgcttgaggg aaacaatgtc tgtgccgatg ccgtaaatca
     3001 tatttgcgct ccttcgccct tggttgcggg tagtgatgaa tgtgatgatg aatgccgtca
     3061 atttcttggg gcgttaaaat ggcgtgaata tccacttttg gggttttgtc ggtaacaatt
     3121 ttcactgtaa ccttttgcat ttgaaattct aaaacatcgt gccaattatt taataaggag
     3181 ttaaggcatt taataatgaa ttaaagtttg attaaacccg aatgcccgat tttcagagcc
     3241 aataccaata aaacaataaa aattaacatt ttaaaaaaat ttgggctaat cttagtcctt
     3301 aaccgaattc caacatacaa tccaataaca gacaatacgg acagtaaaaa tattaaaccg
     3361 tattcactct tatttaataa ccaatactgg tctcttagca tatatatttg aacaattttc
     3421 gccaaaagat agcatagatt gcttgatttt acgatacgat ttttattttc tgtttcgcta
     3481 agcaaaaata ttaacaatat gggagacatg gcattggttg aaccgccgat gatgcctgcc
     3541 aaaaacccaa aaagaaccat attcttatta ttggcaacta cttgaatatt ttttgctttt
     3601 gcacatacat ttaaaatacc attgacagaa taatacaatg taatgattgc catcagtaaa
     3661 agcagccaag acactggaag tatcaaaagc aacttcaccc ccaaaatgct gccaacgacg
     3721 ctgccgatag caagcaattt ataggttttt aaataataaa caatctcttg ccaaaaaccc
     3781 tttttgttat tgctgcatag aaccaacaag ctcattaaca ggcttggtaa tgccaccaag
     3841 gcaacaacct tagacaatgg catgataaaa gccaatgcgg ttgtaccgag catcggaaat
     3901 cccatgcctg taattccgtg cagtattgcg gcagcaacaa aaacgataga ttgcattatt
     3961 tcttgcataa ccgtccttta tacagtaatt cttgctctaa tataatgcct gtttttatcc
     4021 gtataatcag ttctggcatg caagccattg acattgtcaa taataatcaa accatcatct
     4081 tgtgcagaaa attcttgaat atcactcgca ttttttataa catttacaag ataatcaagt
     4141 gcaagtacca tttcctttga tactttattt ctattttctt caataccttt atagatacaa
     4201 tcacttctaa atctaatcat ttgtgtattg agcgataaga tattacccca tatcacacct
     4261 tgttttttat caaatgatgt tggcgtttta aatggatata aattgcccgt taatgttttt
     4321 aagtgttttt taccggtttc tgttttagat aactgattga caatatctga tgaacttaaa
     4381 aatagggaat tacctccatc attggctgat tttactacat acataaccaa ataactttcc
     4441 gggttttcac taaaagatga atcactatga agtggacatt ctccaacatc ttccgagaaa
     4501 gttatatca
//
LOCUS       DAXHKX010000158         4505 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHKX010000158 DAXHKX010000000
VERSION     DAXHKX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.158"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..375
                     /locus_tag="ACFMWZ_001783"
     CDS             <1..375
                     /locus_tag="ACFMWZ_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC5935705.1"
                     /translation="AQISGKRYQRLSLVSAQVGNRLIAPMVYQNTMTGVFFEAWFQQC
                     LLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSPELNPIEKVWANIKRY
                     LRTVLSDYARFDDALLSYFDFN"
     gene            complement(539..1942)
                     /locus_tag="ACFMWZ_001784"
     CDS             complement(539..1942)
                     /locus_tag="ACFMWZ_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC5935706.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(2011..3438)
                     /locus_tag="ACFMWZ_001785"
     CDS             complement(2011..3438)
                     /locus_tag="ACFMWZ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC5935707.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLILNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3674..4027
                     /locus_tag="ACFMWZ_001786"
     CDS             3674..4027
                     /locus_tag="ACFMWZ_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935708.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4006..>4505
                     /locus_tag="ACFMWZ_001787"
     CDS             4006..>4505
                     /locus_tag="ACFMWZ_001787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935709.1"
                     /translation="MEGIMLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFL
                     GFFSDGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHPARYAVTFENNVDSKLRRH
                     WVAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNKERLTSSEPLS
                     DFVLSV"
BASE COUNT         1066 a         1178 c         1108 g         1153 t
ORIGIN      
        1 gcgcagataa gtggaaaaag ataccaacgc ttatctttgg tgtccgcaca agtcggcaac
       61 cggctgattg ctccgatggt ttatcaaaat acgatgaccg gagtcttttt tgaagcgtgg
      121 tttcagcaat gcctactgcc cgcattgact caaaaatcgg tgattatttt agataatgcg
      181 cgatttcacc gtatgggtgt cttacgggaa atggcggaaa aattgggaca taaggtattg
      241 cctcttgcac cttattcacc tgagctcaac ccgattgaga aagtgtgggc gaatattaag
      301 cggtatctgc gaaccgtttt gtctgattac gcccgatttg acgatgcact actgtcctat
      361 tttgatttta attgaatata catttgaatt aattcgcact taatttaaat gtgtttttaa
      421 ctgtgcttta tttaaaggca atgagaatgt gaaagtatcg gatcaatccc aaagcagcct
      481 gcactttcga aacggggtgc aggctgcttt gggaatttca taaccgtttc agcctgcttt
      541 attccgcaaa taccgtttcc aaccctaacc cgctctcttt caccaagcgc aaataagcca
      601 gcatgaattt ataccgtgct tgagccagtt tctgttctgc ttgggcgact tcctgccgcg
      661 cccgtattac ttccagccgg ttgcggatgc cgtattgttg gccggtttcg gtcgatttca
      721 gtttcaaacg gctgctttcc aaaacccgtt cttgcgccat gatttggtaa cgcgccgcac
      781 cgctttcggt ataagcctgg cgtacggcga gtttgatgtg ccgctcggtt gcggtcagct
      841 gtgcttcggc ggccccgtat tgcgcttcgg cttcatggat tttgcccgac aattctccgc
      901 cggtataaag cggcaaattc aactgtacgc cgacgctcat ccctttgccc cgatagtggt
      961 agtcattatt ctgcgcagat gaagtgtaga ggttattctg atagccgaca tgggcagaaa
     1021 cggtgggata gcggctgttc tgtgctgccc gaagcgcctg tccgctgctt tgcagggcaa
     1081 gctgctgcat ccggtattca tgattgttgg ataaggcaat gcgctgccat tcatccagac
     1141 tgtaacgttc cagcttgggc agatagcgtg ccaacaggtt ggcggtatct atggcctcga
     1201 tttgtttgct atccaggtcg gtgtagtcgt tcaactggtt ttcataggtt tgtttctcag
     1261 ccaatacggc gatttcttgg gccagggcat tgtcgtaacc ggctttggct tcgtgaatat
     1321 ccagcgcggt ggcagcacct ttattgaata aagcctgcgc ctgccttacc tgctgggcat
     1381 aagcctcttt ttccgccgca tgggcggcaa cggtgtctcg gctgagtaaa acgttgaaat
     1441 aactttcggc aactttcaac agcaattctt cgcgtgccgc atcgaaacgc tgttctgcag
     1501 cctgcgtatc gaacctgctt tggcggtatt gtgcaaattt ggcagcgtca aataaggttt
     1561 gtcccacctg cacgctccat ccctgtgttt cgcgggtgga agaaatcgat ggcggctggc
     1621 gctggtagct ggcattggcg gatacatggg gaaggaatgc ggccttggct tgttgttgcc
     1681 gtgcgcgcac tgcatcacgc tggtaatggg acgcttgaaa atcagccgaa tgttgctgcg
     1741 ccgcccgcca tgcttcaggc agcgtaaaag ccgaaacgga tggggaaagg gatagtggca
     1801 aggtaaaaag tgaaacgggt aggatatatt tggaaaaata ggatttcata gccgaaaata
     1861 gttcatgttg caaatagggc gtcagtgtca ggcaaacgga aataccgtaa tcttgcatta
     1921 tcattagatt gagcaatgtc atccgggcaa tggtttcagg cagtctgcat gtccgaaccg
     1981 gcggataaca aatgcccagt acggatccgc ctatcgctcc ctaaagcttt cgtccaattt
     2041 ggtttgcagc gggcttaaca gataatccag cacccgccgt ttacccgttt taatctccgc
     2101 cgtgacattc atgcccgccg tcagattcac tgctttgccg tcaatattca aggtatgttt
     2161 gtccagcgac accaccgccg tataaaccaa gcccaactgt tcgtggctta ccgcatcatg
     2221 gctgacactt ttcaccttgc ccgtcagata accgtagcgc gtatagggaa aactctcaat
     2281 cttcaccacc gcatcctgtc cctgttccac aaaaccgatg tctttgttca atatcaaaac
     2341 ttccacgtcc attttgtcgt catcgggcgc aaccaccatc attttttggg cagcctgcac
     2401 cacaccgccc accgtatagg tggccaattc ctgcaccgtg ccgtccgcag gcgactgtat
     2461 tgtcatcagc tgctgccgct gctttgcctt atccgtttgg ccgcggtatt ggtcaatctg
     2521 ttcgtttgcc tggcgcagcg catccagcgt atcgcgtttc aggttctgcg tattcagcac
     2581 ccgactctgc tccgcctgtg caatggccgc ctgaatctgc ctcatctgac cgcgcgtact
     2641 ttccaaatcg ttccaattgc tgaccgattt gctctgctgc tccaaaaacg catgttccga
     2701 aataaaattg tcggcccgca aacggcggta gtctgctgtt ttctgctgct cgatcgcccc
     2761 caccgaaacc agcttctgct cctgcgcctt ggccgactgc aattccgcct gatggccgcg
     2821 caaagccgac tgcaattgcg catcctgcgc cgcccatgcc tgatactggt gctgcgccaa
     2881 cacctgcgcc gattgcacat cggcatcgga gagacctaaa gaccgtgctt gcgccatatc
     2941 gatatgcggc acggtacggc tttccaatgc cgccaatacc gcttcataac gcagtttgga
     3001 caattgggca gcctgcaaag cctgctccga ctgcaccaca tcgctgtctg ttcccacagc
     3061 ctccagttcc gccagcgttt ctccctgttt cacatgctgc ccgtcgcgca catgtaccgc
     3121 cttaaccacc gccgtttcca gcggctggat ggttttgctg cgcccgcccg acaccgtttt
     3181 gcccgaagcc gccgccacaa tatcgatttt gccgaaccag gaccacaaca aagccaaaag
     3241 cgcaaacgcc ataataaaac gcgccgccca tttcggagcg gcagagaccg gcgtatcggt
     3301 cagttccaaa tgcgcgggca aaaacgcctg ttcttccgcc gtgcgtttgg gcggttccaa
     3361 ctggtcgcgc accgcccaaa cattgcgcca tacagtaatg tatcgagaaa gaaaggattt
     3421 cagggcggag aaaaacataa cgggtataac cttggcaata tagaaacagg aaacaatata
     3481 aatatgtaaa ggaattttaa cggaaagcgc ggcagctgtt aagggaaagg cgggaatatt
     3541 gacaaaaaat acccaagtcg ttacaaatat tcattatttt actgcgtaac gcaacgctga
     3601 agcgcaggct gcttttgaga tgcggcaagg ttcggcaaaa agcagcctgc acatttaacc
     3661 acaggaacaa cccatgttta ccacaaacga tttacgccat ttcctagaag gtttggccat
     3721 cctattctca atcggctatt ggggcaccat gctgctgttg ctttggtttc tcgtccgctt
     3781 tgcctataaa aagcccaaac ggaaccccgg caaaatcctt tcaggcagcc tgaccgccgc
     3841 cgctatcctg atgctgtttg tttggattat tcccaaacaa ttcggcccga tcaaagaaga
     3901 aatacaggca caagaagagt gggacagaaa atacaaagaa gccgaagccg tgtttaacga
     3961 acaatgcaaa acggcggggg aaagatttac cagacggcgg acaatgtgga agggattatg
     4021 ctgttgaagg tagtacctga gcgtaccgtt tcggcagatg caaaaaccag agacccgatg
     4081 tgggacaatg cggctttaca gaccagcgaa ggcgtaaatt ttattgctcg tttcctagga
     4141 ttttttagcg atggggaata ccgctatgtg gatgtcctgc aacccaacca ttccgatatt
     4201 attcggtatt caggtaaaga ttttccgata aatcaaatac ttaatcatat acaccccgcc
     4261 cgttatgcgg taacgttcga aaacaatgtc gattccaagc tgcgcaggca ctgggtggca
     4321 ggtgcgacca tacggattat cgaccgccaa actgacgaag tgattgccaa gaaaaccatc
     4381 tatgtctttg aaaaaggctt ggacggcacg ggtggggcga gaatgccgtg gaagtttgct
     4441 atcttgtgca ataaagaaag acttacttct tcagagccgt tatcggattt tgttcttagc
     4501 gtttt
//
LOCUS       DAXHKX010000159         4505 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHKX010000159 DAXHKX010000000
VERSION     DAXHKX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.159"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(104..1042)
                     /locus_tag="ACFMWZ_001788"
     CDS             complement(104..1042)
                     /locus_tag="ACFMWZ_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC5935710.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1051..1926)
                     /locus_tag="ACFMWZ_001789"
     CDS             complement(1051..1926)
                     /locus_tag="ACFMWZ_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC5935711.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWVERVKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLALGWHCAGLLDGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPNK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLFPLIRPVSQLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2122..2198)
                     /locus_tag="ACFMWZ_001790"
     tRNA            complement(2122..2198)
                     /locus_tag="ACFMWZ_001790"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2162..2164),aa:Met,seq:cat)
     regulatory      2348..2447
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2538..4439
                     /gene="thiC"
                     /locus_tag="ACFMWZ_001791"
     CDS             2538..4439
                     /gene="thiC"
                     /locus_tag="ACFMWZ_001791"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC5935712.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGGNVTQLHYARRGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTRPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1093 a         1299 c         1184 g          929 t
ORIGIN      
        1 gttgtttcgg ataaattttg cagccctgat aaaaaatatg gctgctttgg taaaaaaatg
       61 ccgtctgaaa ggttttcaga cggcattttg tttttaagaa gcatcaacgg aagcggacga
      121 tttccctgtc ttcgatatgg atgcgtaccg tatcgtttcc cgatatttca ccggcgtgcg
      181 gcatatcgag gttcagccac aggatgccgt gttccggatg gaggacggac aggctgaacg
      241 attcgggcaa acaggtacgg gataatacgc ggcactccat gccgtcttgg tcgaaacgca
      301 ccgcatgttg cggaatatgg cggtcatcgt cggtattggg caaacccatc agtcgggcga
      361 cctgcacgcc ggcaggcgtt tgaaccaagg tttcgggcgt accgcattga aggattttcc
      421 cctcgtgcat gacggcgatt tcgtccgccg ccgtgcaggc ctcttcgggc gaatgcgtca
      481 ccaaaacggc agggatgccg cccttgcgga tacgttcggc agtcatgcgg cgcagccggt
      541 cgcgcaaatg cgtgtccaaa ctggaaaacg attcgtccaa cagcagcagg gaagggcgga
      601 caaccaaagc gcgcgccaac gccaaccgtt gcttctcgcc tccggaaagt ttttcaggct
      661 tgcggtgcgc ctcgttttcc agtccgactt cggcaagtgc cgccatggca aggcgttcgg
      721 cttcggcttt cggcattttt tgcattttca aaccgaatgc cgcattttcc agcgcactca
      781 tatggggaaa cagcgcgtaa tcttgaaaca tcagagaaat acggcgtttt tcgggcggca
      841 tacgggtaat gttttccccg ttcagccata tttccccacc gtccggccgg acaatccccg
      901 caatcatatt cagcagggtg gattttccgc atcccgaccg ccccagtacg gcgagtattt
      961 tgccgcgccc gacagtcagg cagatgttgt cggcaaccgt tttgccgccg aagcgtttgc
     1021 agagtccgtt cagttcaagc atggcgcatc ctataaacgt atgccgtcca gccactcgga
     1081 cagcggatgg gcggcatcaa tcagaccgta acggcaaaac gcgtccagcg taaccagttg
     1141 cgcgtcgtgc atcatgtttc ccgacaacat ggtggcaacc aagctgccga tgtccatttt
     1201 ttcaaaacac gcaacctcgc cgtcctgatt ttcaggcagg aaggtttcgg gtaggacggc
     1261 atcgaataca tacaggattt cattgtgcac accccggctg acggggcgca ggctgtgcag
     1321 ctgcgatacc gggcggatga gcggaaacag cgttttatcc aaaccggctt cttcgctgct
     1381 ttcgcgacac acggtttcag acggcaattc accgctggaa acaccgccgg cggcagtatt
     1441 gtcgagtttg ttgggatcga ctgctttgtg cggactgcgc ctgcctatcc agaaatgcca
     1501 tcggccgtcc gattcggtca gaccgttgag atggacggcg cggctgagca gtccgaaagg
     1561 acggaaagcg gcgcgttcga gcgtgaacaa ggggttgccg ccgccgtcgg tcaggtcgaa
     1621 acactcgttg cgccagccgt ccaacagccc cgcacagtgc caaccgaggg cgaggtgctg
     1681 taagcgtccg cccatatcag gccagccgtc cgcattcaga aaaatgccgt ctgaagactc
     1741 cgagcagcct gcctcccagt cttttttgac gcgttccacc cattccggcg acaggttgcc
     1801 caaaggcaga ccgttcagat acagcgtttt ccagcaactt tctgcaccgt aacttgcttt
     1861 tgcccactcg aacagagcat caaggtcttg tttgctgacg gattcggtaa aacggacggt
     1921 cggcatggtt ggctttcttg aagacaatgc ggaagattgt agccaaactg cctgaatttg
     1981 caaccgcaaa cagacggcgg tctgccgttc cgatattgcg gccggaagga aaatccggca
     2041 aaaaacggag gcggcaggaa acaggaaaca ggaaacaggc aaaacaaaac ccctcggcat
     2101 tgaaccgagg ggtttgatat ttggttgcgg gagcaggatt cgaacctacg accttcgggt
     2161 tatgagcccg acgagctacc atgctgctcc atcccgcgtc agaagatgaa actatacggc
     2221 agatttttta tgttgtcaaa cattatttcc gcgaaaaatc ataaaatttt gccgtccggc
     2281 ggtttatgcc tgatttgaaa tatcattttc tttacaaaag ttcatgttcg tgatttaatt
     2341 ttggttaaca tcgaaacagg ggtgctgcct gatgttcagg cggctgagaa atacccttta
     2401 cacccgatcg ggataatacc tgcgtgggga gttttcacgg attctgtctt tcagacagca
     2461 ttggttttca aatgccgtct gaaaacgcaa aacgctcctg tttctttatt ctaaacgaga
     2521 aaacaggagc attttttatg actacgccaa aaaaaaccgc caaaacttcc ggcaacgaag
     2581 cgcgcgaact tgccgacttg agcgaagaca tcggtatccg cttcaaatat ccgaactcgg
     2641 aacgcgtgta tctgcaaggc agccgcgacg acatccgcgt gcctttgcgt gaaatccgtc
     2701 aggacgacac ctatacggcg caaggcgcgg aagccaaccc gccgattccc gtctatgaca
     2761 ccagcggcgt gtacggcgat ccagcggcac acatcgacct gaaacaaggc ctgccgcaca
     2821 tccgcaccgc atggctgaac gaacgcggcg ataccgaaat cctgcccaag ctctccagcg
     2881 aatacggcat cgaacgcgca cacgatccga aaaccgccca tctgcgcttc aaccaaatca
     2941 cccgcccgcg ccgcgcgaaa agcggcggca acgttaccca gcttcactac gcgcgccgcg
     3001 gcatcatcac gcccgaaatg gagtttgtcg ccatacgcga acgtttgaaa ttagacgaat
     3061 tgtcccaaaa accggaatac gccaaactct tgaagcaaca cgcggggcaa agtttcggcg
     3121 cgaacatccc gacccgtccc gatcaaatca cacccgaatt cgtgcgccgg gaaatcgccg
     3181 ccggacgtgc gattattccc gccaacatca accatcccga actcgaaccg atgattatcg
     3241 gccgcaactt ccgcgtcaaa atcaacggca acttgggcaa ttccgccgtc acttccagcc
     3301 tgaccgaaga agtcgaaaaa atggtgtggt cgctgcgttg gggcgcggac acaattatgg
     3361 acttatccac aggcgcgcac atccatgaaa cgcgcgaatg gattatccgc aacgcgcccg
     3421 tccccatcgg caccgtgcct atttatcagg ctttggaaaa aaccggcggc attgccgaag
     3481 atttgacttg ggatttgttc cgcgacacct taatcgagca ggcggaacaa ggcgtggact
     3541 atttcaccat acacgcgggc gtgttgctgc gttatgtacc gatgaccgcc aaccgcctca
     3601 ccggcatcgt ctcgcgcggc ggttcgatta tggcgaaatg gtgtttggca catcatcggg
     3661 aaaacttcct ctacacgcat ttcgacgaaa tctgcgaaat catgaaagcg tatgatgtgt
     3721 cgttcagcct cggcgacggc ctgcgccccg gctgcattgc cgatgccaac gacgaatccc
     3781 aattcgccga gctgcacacc ttgggcgaat tgaccgataa agcgtggaaa catgacgtac
     3841 aagtcatgat cgaaggcccc ggccatgtgc cgctgcaacg cgtcaaagaa aacatgaccg
     3901 aagagctgca acactgcttt gaagcacctt tttacacact cggcccgctc gttaccgaca
     3961 tcgcacccgg ctacgaccac atcacctcgg gcataggcgc ggccaatatc ggctggtacg
     4021 gcacggcgat gctttgttac gttaccccca aagaacattt ggggctgccc gacaaagaag
     4081 acgtgcgcac cggcatcatc acctacaaac tcgccgccca cgccgccgat ctcgccaaag
     4141 gctggccggg cgcacaatta cgtgacaacg ccctgagcaa ggcgcgtttc gagttccgtt
     4201 ggcgcgacca atttcgctta agccttgacc ctgaacgtgc cgagagcttc cacgacgaaa
     4261 ccctgcctgc cgaaggcgcg aaaatcgccc acttctgctc aatgtgcggc cccaaattct
     4321 gctcgatgaa aatcacgcag gaagtgcgcg actacgccga caagcaaaaa gcccaacggc
     4381 agggcatgga ggaaaaagcg gtcgagttcg tcaaaaaagg cgcgaagatt tacagttgaa
     4441 acgtcaagca aaaaatgccg tctgaaaccc ggaaaaaagg cttcagacgg cattctttcg
     4501 cttgt
//
LOCUS       DAXHKX010000160         4382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHKX010000160 DAXHKX010000000
VERSION     DAXHKX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.160"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(69..2045)
                     /locus_tag="ACFMWZ_001792"
     CDS             complement(69..2045)
                     /locus_tag="ACFMWZ_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC5935713.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEGIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGETGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFMWZ_001793"
     CDS             2194..3033
                     /locus_tag="ACFMWZ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC5935714.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDESACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3233..4264
                     /gene="gap"
                     /locus_tag="ACFMWZ_001794"
     CDS             3233..4264
                     /gene="gap"
                     /locus_tag="ACFMWZ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC5935715.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1113 a         1463 c         1015 g          791 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccggttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cattggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccaaac cggcggcttc caatacttcc
      661 cggcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacgcgc
      721 gcgatgtcca aatcgagtgc gaaatacggg ataccctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatgat cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatta atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aagatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccaccca gcccgaacac cagccgcctc
     1801 attgccctca gcttgggcag cgaaaactcc aagcctatac tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgttgcatga cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggtc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga atccgcctgt atcggctgca ccgcctgtat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgtcgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccggccgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa cccctcaaca aaggaacaaa ccatgggcat
     3241 caaagtcgcc atcaacggct acggacgcat cggccgccag gttttgcgcg ccatctacga
     3301 ttatcagatt caagaccaac tccaaatcgt cgccgtcaac gccagcggca gccttgaaac
     3361 caacgcccat ctgaccaaat tcgacaccgt acacggacgc tttgaagccg acgtatccca
     3421 cgacggcggc aacctcatcg tcaacggcga caaaatcccc ttcttctcga cccgcaaccc
     3481 tgccgaactg ccgtggaaag aactcggtgt cgatttggtc atggaatgca ccggcgcgtt
     3541 caccagcaaa gaaaaagcca aaatccacct cgaaagcggc gcgaaaaaag tcctcatttc
     3601 cgcaccgggc ggcgacgatg tcgatgcaac cgtcgtgtac ggcgtgaacg acagcgtcct
     3661 gaccgccgac atgaccgtcg tttccaacgc ttcctgcacc accaactgcc tctcgccggt
     3721 tgccaaagtg ttgagcgaaa gcgtcggtat cgtcaaaggc gcgatgacca ccatccacgc
     3781 gctgaccaac gaccaaaccg ttaccgacgt gcgccacaaa gacctgcgcc gcgcccgcag
     3841 cggcgtggaa aacatgattc cgaccaaaac cggcgcggca aaagccgtcg gtttggtact
     3901 gcccgaattg aaaggcaggc tcgacgggct tgccatccgc gtgccgaccg tcaacgtatc
     3961 attggtagat ttgagcttcc aagccgcgcg cgacaccaca gtcgaagaaa tcaacgcact
     4021 gatgaaagcc gcctcggaag caggcgcgct caaaggcgtt ttaggctaca acaccctgcc
     4081 cttggtttcc atggacttca accacaccac cgaagccagc cacttcgatg ccacactgac
     4141 caaagtcgtt gacggcaaca tggtcaaagt cttcgcatgg tatgacaacg aatggggatt
     4201 cagctgtcaa atgctgaaca ctgcacgccg catgttcgga cttgaagtgc gcccgctcaa
     4261 ataagcaata aaccgtcaaa caaaatgccg tctgaaaccc gatgttttca agttccagac
     4321 ggcatttttc accgtgattt tacccggctg tcgtcatttc caattttata gtggattaaa
     4381 tt
//
LOCUS       DAXHKX010000161         4309 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHKX010000161 DAXHKX010000000
VERSION     DAXHKX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.161"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..204
                     /locus_tag="ACFMWZ_001795"
     CDS             <1..204
                     /locus_tag="ACFMWZ_001795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935716.1"
                     /translation="RLVLIFVNPLYNQYIKSLLALLVLSMPSNTVQCNEPFSGSMVGN
                     IFDISKIKSSSINIPSLYWDETL"
     gene            572..2299
                     /gene="ilvB"
                     /locus_tag="ACFMWZ_001796"
     CDS             572..2299
                     /gene="ilvB"
                     /locus_tag="ACFMWZ_001796"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC5935717.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQEFMPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2310..2801
                     /gene="ilvN"
                     /locus_tag="ACFMWZ_001797"
     CDS             2310..2801
                     /gene="ilvN"
                     /locus_tag="ACFMWZ_001797"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC5935718.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2868..3161
                     /locus_tag="ACFMWZ_001798"
     CDS             2868..3161
                     /locus_tag="ACFMWZ_001798"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC5935719.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3256..4269
                     /gene="ilvC"
                     /locus_tag="ACFMWZ_001799"
     CDS             3256..4269
                     /gene="ilvC"
                     /locus_tag="ACFMWZ_001799"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC5935720.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVIANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1121 a         1026 c         1078 g         1084 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tacaatcaat atattaagtc gttgttagcc
       61 ctcttggttc tgtcaatgcc gtccaataca gtccagtgca atgagccatt ttcaggaagt
      121 atggttggga atatttttga tatatcaaaa ataaaatcaa gttcaatcaa tatcccgagt
      181 ctttattggg atgaaaccct gtaaatttta caattcttga tatcgaagaa gggtttttca
      241 catctgtccc tcctacacaa actgtcaaca atttccatct ttcaaaaaag gtactcctga
      301 ttttattctt ttaaaatcaa tcaataattt ctctgctttc aaaaaatatt aaaagatgaa
      361 agatttttct tgattgaact gtcggtctgc gttatcgttt ctactctttt tcacaaactc
      421 tgtgttcctt tccaattgat tggataatcc gtctgttgcc gtgttccccc aatgcgcgta
      481 ccctaaatcg ctgcttggtg gaattgcatt acaggtgctg tggcaaagcg gtttgtccct
      541 attggtttga aaccgtataa aagaggtcgt tatgcagtta tcaggcgcgc aaatcatagt
      601 gcagagtctc aaagccgaag gtgtcgagta cgttttcggt tatcccggcg gtgcggttat
      661 cgaaatctac gatgcccttt tccaactcaa taaattcaag cacattctga cccgtcacga
      721 gcaggcggca gtacacgcgg cagatgcgta tgcgcgcgtc agcggtaagg tgggcgtggc
      781 attggttaca tccggcccgg gcgttaccaa tgcgctgacc ggtattgcta ctgcctatac
      841 ggattcgatt ccgatggtgg tcatcagcgg gcaggtaggc aattccctga ttggtacgga
      901 tgcgttccaa gaagttgata cggtgggtat tacccgtccg tgcgtcaaac acaatttcct
      961 ggttacggac atcaatgagt tggcggaaac cattaaaaag gcgttccaaa ttgccgcaag
     1021 cggccgaccg gggcccgtgg tggttgatgt cccgaaagac gttacgcagg cgatggcgaa
     1081 attcagctat cctcaggaag acatttttat ccgttcgtat caaccggttg ttcaagggca
     1141 tatcggtcag attaaaaagg ccgtgcagat gttggcatct gccaaacgtc cggtcgtcta
     1201 ttttggcggc ggcgtggtgt tgggtaatgc ttctgaagag ctgacccgat ttgtccgaat
     1261 gacgggtgct ccgtgcacgg gtacgctgat gggcttgggc gcttatcctt ccggcgaccg
     1321 ccaattcctc ggtatgctcg gtatgcacgg tacttacgag gcaaaccttg ccatgcagaa
     1381 tgcggatgtc gttcttgccg taggtgcgcg ttttgacgac cgtgtggttt ccgtaccgtc
     1441 caaatttttc gagaaggcga agaaagttat ccatatcgat gtcgatcctt ccagcatcgc
     1501 caaacgcgtg aaggcggaca ttccgattgt cggcgacgtg aaaaatgttt tgtctgagat
     1561 ggttgcgctg tggcaaaaac aagagttcat gccgtctgaa gatgctttgg gcaaatggtg
     1621 gaaaaccata gaggaatggc gttcccgcga ttgcttgtgg tttgacaacg gcagcgaaat
     1681 tatcaagcca caatatgtga ttcagaagct tgccgagatt accggcaatt cggcaatcat
     1741 cacatcggat gtagggcagc atcaaatgtt tgcggctcaa tattatccct tcgaacgtcc
     1801 gcgccaatgg ctcaactccg gcggtttggg tacgatgggc gtgggtctgc cttatgcgat
     1861 tggtgcaaaa cttgccgccc cggatcaaga cgtattctgt atcaccggcg acggctcgat
     1921 tcagatgaac atccaagagt tgtccacctg tttccaatat cggattccgg ttaacgtcat
     1981 tacgctgaac aacggttatc tcggtatggt acgccagtgg caggaaatat attacggcgg
     2041 ccgagagtcg gaaacttatt tcgattcttt gcccgatttc gtcaaacttg ccgaggcata
     2101 cggccatatc ggtatccgcg tggacaagaa gtctgatgtg gaaggtgcct tgttggaagc
     2161 attgaaccaa aaagacaggc tggtgtttat cgacttcctg actgaccaga aacagaatgt
     2221 gatgcctatg gtcggcaacg gcaaaggttt ggacgaaatg gtacttccgc cgcatatgcg
     2281 tgcggacgga aaggcgtaag gagagacaaa tgcgacatat cttatctgtt ctgatcgaaa
     2341 acgaatcagg tgcgatgagc cgcgtggtcg gtttgttctc tgcacgcgat tacaatatcg
     2401 attctttggc ggttgcgccg accgaagaca aaaccctgtc ccgtatgacc atcgttaccc
     2461 acggcgacga gcaggttatc gaacaaatca ccaagcaact caataaattg attgaagtga
     2521 ttaaagtggt cgatttgaac gaaagccgtt ttgtcgaacg agaactgatg ttggtaaaag
     2581 tccgtgccgc cggcaaagac cgcgacgaat ttttgcgttt gaccgaaatc taccggggca
     2641 gcatcatcga cgtaaccgac cgcagctata cgattgaaat taccggctcg accgacaagc
     2701 tggattcctt tttggaaacg gtcggacgcg cccaaatttt ggaaaccgta cgtacaggcg
     2761 cggccggcat cggtcgcggt gagcgtattt tgaaaattta acgccgtaac ctttcagacg
     2821 gcacggtatt tgaatgccgt ctgaaaaacg aacggcagga gagatttatg tcgaacatta
     2881 aaattgtcgc actggttacc gtcaaaccgg aatacacaga aacactggca gcacagttta
     2941 aagaactggt caaagccagc cgtgcagaag agggcaacat cagctacgat ctccatcagg
     3001 aaatcggcaa accgaaccgt tttgttttcg tggaaaattg gaaatctcaa gcagctattg
     3061 acgaacataa cgccagcgcg catttccaag ccttcgtcca gtccgtcgac ggcaaaaccg
     3121 aagcgttgga aatcgtattg atgaatgaag ttgccgatta agcggtacac tctgtttaac
     3181 ccgtccgaag ccgctcaaca ttttaaggct tcgacaacta tttaccttac aatcatttac
     3241 cttaaaggaa atcaaatgca agtctattac gataaagatg ccgatctgtc cctaatcaaa
     3301 ggcaaaaccg ttgccatcat cggttacggt tcgcaaggtc atgcccatgc cgccaacctg
     3361 aaagattcgg gtgtaaacgt ggtgattggt ctgcgccaag gttcttcttg gaaaaaagcc
     3421 gaagcagccg gtcatgtcgt caaaaccgtt gctgaagcga ccaaagaagc cgatgtcgtt
     3481 atgctgctgc tgcctgacga aaccatgcct gccgtctatc acgccgaagt tatagccaat
     3541 ttgaaagaag gcgcaacgct ggcatttgca cacggcttca acgtgcacta caaccaaatt
     3601 gttccgcgtg ccgacttgga cgtgattatg gttgctccca aaggtccggg tcataccgta
     3661 cgcagtgaat acaaacgcgg cggcggcgtg ccttctctga ttgccgttta ccaagacaat
     3721 tccggcaaag ccaaagacat cgccctgtct tatgcggctg ccaacggcgg caccaaaggc
     3781 ggtgtgattg aaaccacttt ccgcgaagaa accgaaaccg atctgttcgg cgaacaagcc
     3841 gtattgtgcg gcggcgtggt cgagttgatt aaagcaggtt ttgaaaccct gaccgaagcc
     3901 ggttacgcgc ctgaaatggc ttacttcgaa tgtctgcacg aaatgaaact gattgttgac
     3961 ctgattttcg aaggcggtat tgccaatatg aactactcca tttccaacaa tgcggagtac
     4021 ggcgaatacg ttaccggccc tgaagtggtc aatgcttcca gcaaagaagc catgcgcaat
     4081 gccctgaaac gcatccaaac cggcgaatac gcaaaaatgt ttatccaaga gggtaatgtc
     4141 aactatgcgt ctatgactgc ccgccgccgt ctgaatgccg accaccaagt tgaaaaagtc
     4201 ggcgcacaac tgcgtgccat gatgccttgg attactgcca acaaattggt tgaccaagac
     4261 aaaaactgat tgttttcaaa cgggactgcc tacacatcgt gtaggcagt
//
LOCUS       DAXHKX010000162         4303 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHKX010000162 DAXHKX010000000
VERSION     DAXHKX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.162"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(299..829)
                     /locus_tag="ACFMWZ_001800"
                     /pseudo
     CDS             complement(299..829)
                     /locus_tag="ACFMWZ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1837..2607)
                     /gene="xth"
                     /locus_tag="ACFMWZ_001801"
     CDS             complement(1837..2607)
                     /gene="xth"
                     /locus_tag="ACFMWZ_001801"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC5935721.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(2661..2948)
                     /locus_tag="ACFMWZ_001802"
     CDS             complement(2661..2948)
                     /locus_tag="ACFMWZ_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFC5935722.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            complement(3354..4235)
                     /gene="nadC"
                     /locus_tag="ACFMWZ_001803"
     CDS             complement(3354..4235)
                     /gene="nadC"
                     /locus_tag="ACFMWZ_001803"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC5935723.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAILVKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
BASE COUNT         1032 a         1067 c         1078 g         1126 t
ORIGIN      
        1 gcatttatcg aaaataactg aaaccgaaca gactagattc ccgcctgcgc gggaatgatg
       61 aattcatccg cacggaaacc tgcaccacgt cattcccacg aacctacatt ctgtcattcc
      121 cacgaaagtg ggaatccagg attcagggac ggagaaacct tttttcccga taagtttccg
      181 caccgaccgg tctagattcc cgcctgcgcg ggaatgacgg ttcgggtatt tctgacgatt
      241 catcgagccg tagcaactgt atttttcacc ccgtcgggca aaaataccaa aactcaaatc
      301 aagccgtccg gataccgttt tcggcggtat cgttttcggc ggtatcgttt tcggcaaaat
      361 aatcacgcat ccgggcattc gatatcgtca gcagtttgcg catacatgcc gtaacggcaa
      421 ccttatacgg cttaccctcg gacagcaggc gttggtagaa atcccgaata agcggttcaa
      481 aacgtgtcgc tgccacggta gccatataca gcgccttacg taccgcagac tttccgccaa
      541 agcagcggct tttgaatttg gtttccccgc tctccctcgg gtgcggggca atgccggcca
      601 aactcgctat ccgtttgtgc gacagccgcc ccaattcggg cagcatcgcc atcagcgtag
      661 ccgtcgttat cgaaccgatg cctttgattt gctccgccac ttgggctttg ccgtcaaaat
      721 gcgtgtgggt gtggtcgtcg atttgtttgt ccaattcgtc aatcagccgg tcaaaatggg
      781 caatcagttg tttgacgctt ccgacttgcg tttcatgaaa ctgatgcaga tatagtggat
      841 taacaaaaat caggacaagg cgacgaagcc gcagacagta caaatagtac ggcaacgctg
      901 tactggttta aatttaatcc actatatttt gtaatagctg ccattatgcc tactaggata
      961 tataccttct atataatcct tatttaaata aaaaccctta taaaaatctg tgttatctgt
     1021 cgggattgaa aaaaagattt tatctttctc atcaggttta tatttgctat caatatcaaa
     1081 aagagaataa ggattacaat gagttcgaca tactaggtaa aaatttatat cttggatcat
     1141 gaaatattcg atatttatca ggatcgaaag atgctaaaaa ttcatcaata tcggtactgg
     1201 atattttgtc atcactaata aacatcgcta gagatgggcg actttgattt tccatttgtt
     1261 gattagtaaa ggatataaat actaacttgc cattgtcaaa ccacatagat ttatatagtg
     1321 gattaaattt aaaccagtac gtcgttgcct cgccttgccg tactatttgt actgtctgcg
     1381 gcttcgtcgc cttgtcctga tttttgttaa tccactatag ttattgcctt tgggagaatc
     1441 aattctgaca gcacataaca taggttcatc tttacagtcg ttatattggg tttcatcaaa
     1501 ctcaactgca tagcacaata aaggcaatag gaaaaaaagg agattaattt attactatac
     1561 ataaggcagc ctgactattc attctaaaat aaatccggtt tttaatattt gattctgctc
     1621 cggattatat acataataaa ttttactgct ccaaaatctg atattaaccc tatggatttc
     1681 ataaattttt cagaaaaata ggctgtgctt gaacaatccg ccgactccgg ctgtttgtca
     1741 gggaaaaggc aattattttc ggttagtcag cagcctatcc ttttgaatca cttaaagata
     1801 tggatgtttg tcaacgccat atcaaaacac ggtcttttac caatcgaatt ctgccgtcac
     1861 cggcgcgtgg tcgctcggac gctccagcgc gcgcgtctcc aaatcaacgc ggacatcctt
     1921 caacgccgcc gccatcgcag gcgacaccaa aatatggtcg atacgcaggc ccagtttgcg
     1981 ttggaacatc gcgccgcgat agtcgaacca ggtatagaac gcgccttcgg gatggacttg
     2041 gcgcaggctg tcggtcagtc ccaaatccag caggtttttg aaccactgcc gttcgacgga
     2101 cgaacagtgg attttttcgt gccatttttc agggtcgtaa cagtccgcat cggcaggcgc
     2161 gatattgaaa tcgcccagta gcaccagttt gccgtagcgg gtcatttcat cgcggacaaa
     2221 ttcggttaac gcggcaaacc attgttcttt atatttgaat ttcgggctgt cgagggcttc
     2281 gccgttgacg caatagacat tgatgacgcg cacaccgccg acggttgccg caatcacgcg
     2341 ccgttgcgga tcgtccggca gcgcgggcag cccgatatgc acgtcttccg gcgcgttgcg
     2401 gctgacgatt gccacgccgt tgtaggtttt ctgcccgctc caaacacagt gccagcccat
     2461 catttgcaaa gcggcggccg gaaatttgtc ctgatcgagt ttgagttcct gcaaaaccaa
     2521 aatatcgggc ggattgtcgg caagcaggtt ttgcacctgc ggcagccgca cattgagcga
     2581 attgacgttc caagtggtga ttttcataac atttccgaaa aatgccgtct gaaacgttca
     2641 gacggcaaaa ggaaaggatt ttaacaccat accgcgcgtt tgttttccaa atcgcggatt
     2701 gtccgcagaa tcgttgccgc gtcttcggaa atcaggcggt attcgataat gcggtggtaa
     2761 cgcgtaaagt ccaccagacc gcccgccctc aggcggttca aatggccgga aacggcggtg
     2821 gccggcaggg ataaggattt tgccagttcg gcgatattgc gttcgctgtc caacagttgg
     2881 atcagtatcg ccatacgttc gggatgcgcc ataagtttga gtatggtgtg gagcggtatt
     2941 gtgttcatcg gtattcctaa aacctttgcc gcccgccaag cggattgacg ggcgacagat
     3001 atggatgatt gtgattatct ttataaatcg tattataaat caaatattta tggatgattg
     3061 tacaggattg tatgttcctt ttcccacttt ttttgcaaac acgcgtaaag gcgggctttc
     3121 agacggcata aaactgccgc ccccgtcctt tcccctgccc taccgatgcc tccgcgctgt
     3181 cgggcaaaca aaatacctgc ctgaaacgct gttttttcta aagcatcacg accttcacga
     3241 aaaatgccgt ctgaagaata tttcagacgg catttcctcc ttgaggcaat aaccggatta
     3301 cggttaggcg gttccttgtc tgacatatca gacaactgcc gacaccctaa aactcatgcc
     3361 aagaaatcca aacctatgtc caacgagcgg ctgctgtggg tcagatagcc gagggcgatg
     3421 ccgtccacgc cggtttgcgc cacgcgcttc aggcggtcga agccgatgcc gcccgatgct
     3481 tcgcaataga cggtgtgggg gtgggcggtt tgcgtgtggc agcggtttgc cgcttctttc
     3541 agggtttcgt cgtccatgtt gtccagcaaa atccgttccg cgcccgctgc gatggcttcg
     3601 tccagttgtg ccaacgtgtc cacttcgatt tctacacaag tcaacggtcc gaccgcctgt
     3661 tttgcctgcc gcacggcttg ggcgatgctg ccgcaatagg cgaggtggtt gtctttgaca
     3721 aggatggcat cgtccaaacc catgcggtgg ttcacgccgc cgcccgccct gacggcgtat
     3781 ttttgcagga cgcgcagcag ggggatggtt ttgcggctgc acacgatgtc tgtaccgtat
     3841 ttggcgactt cggcaacggc acgcgcggtg gcggtggcga tgccgcttaa gtgcgtgagg
     3901 tagttgagcg cggtgcgttc ggcggcgagc agcgcgcagg cgttgccttc gacggcggca
     3961 agcgtctgac ctgcgcggac gacttgcccg tctcggattt cggcttggaa gcggacggac
     4021 ggatccatcg tctgaaaggc gaggcgcgcc aagtccatgc cggcgataac gccgtcttcg
     4081 cggctgacaa ggaagagttt ggcggttttg tcgggggcga tgacggcggc ggacgtaata
     4141 tcgccgcgcc tgcccaaatc ttcgctcaag gcttgttcga ctatggggcg caacagggtg
     4201 tcgggtaggg aaaagagggt gttttcagac ggcatggtgt ttcctttgtt gaaaaagatt
     4261 gatattgccc gatggggcaa aacttcattt tatagtggat taa
//
LOCUS       DAXHKX010000163         4245 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHKX010000163 DAXHKX010000000
VERSION     DAXHKX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.163"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..571
                     /locus_tag="ACFMWZ_001804"
     CDS             <1..571
                     /locus_tag="ACFMWZ_001804"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC5935724.1"
                     /translation="FPFWKTVKGCVMLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRL
                     NVAGIGEEMDNLKAQAERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGV
                     GNNILEAGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLRE
                     AMGKRLHAHVAALCSDDEIYRTTMAAYTM"
     gene            591..1759
                     /gene="pglH"
                     /locus_tag="ACFMWZ_001805"
                     /pseudo
     CDS             591..1759
                     /gene="pglH"
                     /locus_tag="ACFMWZ_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
     gene            1752..2351
                     /locus_tag="ACFMWZ_001806"
                     /pseudo
     CDS             1752..2351
                     /locus_tag="ACFMWZ_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            2328..3329
                     /locus_tag="ACFMWZ_001807"
     CDS             2328..3329
                     /locus_tag="ACFMWZ_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFC5935725.1"
                     /translation="MPYFTGNQTNEKNNILILSAGRRVELVQDFQTEAARFSDGISVF
                     ATDLNPRMSSACHVSDGSFEVPPISADSYIDSIFNLAVRENIGLIIPTIDTELQKLAD
                     ERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPY
                     DGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAI
                     PRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCITAQFFYNEETGEFYGVEI
                     NPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            3316..4002
                     /locus_tag="ACFMWZ_001808"
     CDS             3316..4002
                     /locus_tag="ACFMWZ_001808"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC5935726.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
BASE COUNT         1054 a         1231 c         1020 g          940 t
ORIGIN      
        1 atttcctttc tggaaaaccg tcaaaggctg cgtcatgctc ggcacgctgt cgcgtctgga
       61 caccgtccgc gccgtgcatc tgatgctgga cattttcaaa aaaatggtcg accgcgatat
      121 gcccgtacgc ctgaacgtgg cgggcatagg cgaagaaatg gacaatttga aagcgcaggc
      181 ggaacgtctg ggcatagacg gcaaagttac cttcctcggc ggcgtgcgcg atttgaccgc
      241 ctacttcaaa gacgtggata ttttggtgaa cacgccccac tgcatcggcg accacggcgc
      301 aggcgtgggc aacaacatct tagaagccgg actttacgac acgcccgtcg ttacctacga
      361 tatggcgggc atttccgaaa tggtcgtcaa cggggaaacg ggttactgca tccccttcgg
      421 cgacgaagaa gcctttatcg ccgccgtaga cacgctcgtc agacaaccgc tgttgcgcga
      481 agcaatgggc aaaaggctgc acgcacacgt cgccgcactc tgttccgacg acgaaatcta
      541 ccgtaccacc atggcggcgt ataccatgta atgccgtctg aaagccgccg atgaacatca
      601 ccatagtcgc cccttattgc tcgctgccgt ccgaaccgta tttcaaccgc ttttggtatc
      661 tggcagaacg attgtcgcaa tcgcacgacg tgttgctgat taccagccgt ttccgccact
      721 acgacaaatc attcagacgg catgaagatg ctgccgccac ttcaaacggc aggctgcgcg
      781 tcaagctgct ggacgaaccc ggctaccgaa aaaacgtctc cctcgcccgc gtcgccagcc
      841 accgcgtatt cgtccgcaat ttggcacgct ggctgcacag cccgcaggcg gcggaacagg
      901 acatcgtcta ttctgcctat ccgctgatgg cgaccaacct gctgttgggc aaacacaaag
      961 cgcgtttggg ctacaaactg attgtcgatg tgcaggacgt atggccggag tctttctcct
     1021 cggtcgtacc attgttgaaa aaagtaccgc acaagctgct gccctttgct tcacgcgcca
     1081 accgtgccta ccgctgcgcc gacgcgctgg ttgccgtatc gcagacttat ctcgaccgcg
     1141 ccaaagaagc caatccgaac gtacccggcg aagccgtcta tatcggtacg gattttgccg
     1201 ccatcgcccc ccccccccgc gattccgctc caaaaccacc cgccttttct acttgggaac
     1261 gctcagttac agctacgacg tggaaaccgt gtgcaaaggt gttcggaaac ttttagacga
     1321 cggcgaaaac gtcgaattgc acattatggg cggaggaccc gatttggaga aactcaaaca
     1381 atatgaaaat cgcgccatca agttttacgg ttatttaccc tattctgaaa tgatgtcgat
     1441 tgccaaaggc tgcgacattg ccgtcaacgc catccatagc cacgccatgc agtcggttac
     1501 caacaaactc tccgactata tggctttgca aaaaccgatt ctgaacagcc agaccaatgc
     1561 cgaagtcctt agcctcttga acctgctgcc gcacgaaaac taccgttccg gcgacgtgga
     1621 cagcttcgtt caagccgcca aagatatttt gaaacgcaaa aacgaccccg ttcagtccga
     1681 tgaaatcgtc cgccgcttca ggcgcgacat ttcctatcgg aaaatcgtca acctgattga
     1741 aagattggca aatgagtaaa gccgtcaaac gcctgttcga catcatcgca tccgcatcgg
     1801 ggctgattgt cctctcaccc gttttcctga ttttggcgta cctcatccgc aagaatctgg
     1861 gttcgcccgt cttcttcatt caagaacgcc ccggaaagga cggaaaacct tttaaaatgg
     1921 tcaaattccg ttccatgcgc gacgcgcttg attcagacgg cattccgctg cccgacagcg
     1981 agcggctcac acccttcggc aaaaaactgc gtgccgctag tttggacgaa ctgcctgaat
     2041 tatggaatgt cttaaaaggc gacatgagcc tggtcggccc gcgcccgctg ctgatgcaat
     2101 atctgccgct gtacgacaac ttccaaaacc gccgccacga aatgagaccg ggcattaccg
     2161 gctgggcgca ggtcaacggg cgcaacgcgc tttcgtggga cgaaaaattc gcctgcgatg
     2221 tttggtatat cgaccattac agtttttggc tggatatgaa aatcctgttt ttgacagtca
     2281 aaaaagtctt gatcaaagaa ggcatttcgg cggaagggga agccactatg ccttatttca
     2341 ctggaaatca aactaatgaa aaaaataata tactgattct gtcggcaggt cgccgtgtcg
     2401 aattggtgca ggacttccaa accgaagccg cacgtttttc agacggcatc agcgtttttg
     2461 ccactgacct caacccccgc atgtcttctg cctgccatgt ttcggacggt tcatttgaag
     2521 tgccgccgat tagtgccgat tcatatatcg acagcatttt caatctggcg gtacgcgaaa
     2581 atatcggttt aatcatccca accatagaca ccgaactgca aaagctggca gacgagcgcg
     2641 aacgctttga agcggcaagt gtccacatcg tcgtttccga ttcggacttc atcgcacaat
     2701 gccgcgacaa acggaaaacc gccaccttgt ttgcccgcta cggcatacgc tcacccgaaa
     2761 tttacgaccg caagaagttg gttttccctt gcttcgccaa gccttatgac ggcagccgcg
     2821 ccatcggtgc aaaacggatt gacacacctg ccgacctgac accggaagtc ttggcagaca
     2881 ccaaattaat gttctgtcag tttatcgaca tcgaaaataa attttccgag tttaccgtcg
     2941 atatgtatta cgaccgtcaa tgccgtctga aatgcgccat cccgcgaaaa cgcttggaag
     3001 tgcgctccgg cgaaatcagc aaaggcgtta cctgcaaaaa cagcctctac caaacgctgc
     3061 tggaaaaaat gtcggtattg cagggcgcgc gcggctgtat cacggcgcag ttcttctaca
     3121 acgaagaaac cggcgaattc tacggcgtgg aaatcaaccc ccgcttcggc ggtggcttcc
     3181 cgctgacgta tgccgcaggc ggcaattatc cgagccggtt gatgcgcgaa tacctcggcg
     3241 acgaagaaat cccgttttca gacgactggg aaaacaacct cattatgttg cgctacgatg
     3301 ccaaggtctt ggttcatgaa aacgattaat cctgaaacca ccgtcatcgt cttcgacttg
     3361 gacgataccc tgtattcgga atacgaatac aagctctccg gtatccgttc ggtcatcgat
     3421 accgttgccg cactgtatcc cgattggaat tcagacaacc tatggcgcaa catcgacccc
     3481 gacggcaaag attggctgga caaattgtgc cgccactgtg gttttaatga atcggaaaaa
     3541 caggttcttt tgtggcaata ccggctgcac cgcccgacgc tgactccgta tgctccgccg
     3601 gatttcctgt ccgaactgac cgcgcccttc gccgcacgcg cgctgattac cgacggcaga
     3661 agcctgaccc aacgcctgaa gctggaagcc ttgggtctgt cttccctgtt tgacgacatc
     3721 ctcatctctg aagcctgctc ttcggaaaag cccgacggca aacgcttccg ccatcttcaa
     3781 gacaaatatg ccgacaaggc aggctgcttc atctatatcg gcgacaacat ttccaaagac
     3841 tttatcgcgc cgaacacttt gggctggata accataggat tgcttccctc cggacagaac
     3901 atccaccgcc accagcctga aagttttaac acagaatatc atcccgactt ttgggttaac
     3961 tcccttcaag atttagttcc tataattgat tcaaatactt aaccattaca taatcaaaca
     4021 tgcactcaca atattaaaag gactatttca tgacaaaata ttattcatgg ggcgtaaacg
     4081 gctttccgcc ctacgttttc gacaccattt ggaaacgatg gaagttgaat gggcgcgggg
     4141 gcggttatcg tgtgcgacat cccggacggc atgaccgtcg cgggcaatcc ggcaaagccg
     4201 ctgccgcgca aaaaccccga gaccgggacg gcataagcga ttaaa
//
LOCUS       DAXHKX010000164         4196 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHKX010000164 DAXHKX010000000
VERSION     DAXHKX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.164"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..217
                     /locus_tag="ACFMWZ_001809"
     CDS             <1..217
                     /locus_tag="ACFMWZ_001809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935727.1"
                     /translation="SLHIENAAQANFQFASLVLALSHGERGLGCRLPLRFRQILTSLI
                     PRFSFAEAALSDDLLEHHRYTHLRNAV"
     gene            complement(228..551)
                     /locus_tag="ACFMWZ_001810"
     CDS             complement(228..551)
                     /locus_tag="ACFMWZ_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC5935728.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTVMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(631..1287)
                     /locus_tag="ACFMWZ_001811"
     CDS             complement(631..1287)
                     /locus_tag="ACFMWZ_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC5935729.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1389..1892)
                     /locus_tag="ACFMWZ_001812"
     CDS             complement(1389..1892)
                     /locus_tag="ACFMWZ_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC5935730.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            2076..2849
                     /gene="tpiA"
                     /locus_tag="ACFMWZ_001813"
     CDS             2076..2849
                     /gene="tpiA"
                     /locus_tag="ACFMWZ_001813"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC5935731.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2856..3206
                     /gene="secG"
                     /locus_tag="ACFMWZ_001814"
     CDS             2856..3206
                     /gene="secG"
                     /locus_tag="ACFMWZ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC5935732.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3220..3305
                     /locus_tag="ACFMWZ_001815"
     tRNA            3220..3305
                     /locus_tag="ACFMWZ_001815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3254..3256,aa:Leu,seq:gag)
BASE COUNT         1175 a         1273 c          907 g          841 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaactttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg ataccgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccag atacatcacc gtatgcaggc tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaaa ccaagcaaaa tgccgtctga agccccaaac
      601 ccgctttcag acggcatcgc aatccgaaca tcaaaactga aaaccgccaa acggatcggc
      661 atccttatca tccaaatgcg ccaccaaggt atcgaacagc accttctctt caaacacatc
      721 gcccttgcgc gtaatcaaaa gcgcgcgctg aacaggcttg cggcctacga taaccgccat
      781 gcgtccgcca tctttcaact gttctttcaa cacttcaggc acaaggttta ccgcaccgcc
      841 gacataaacc gcatcaaacg gcgcacctgc ggaaagttcg gtcaacccgt tgttttgcac
      901 ataatcgata ttgtccaaac ccaagccgtc caacaccgct ttggcgcggt tttgctgttc
      961 ggcatcgatg tcgtccgaca ccacacgacc agccaatttt gccaacagcg cggtcgcata
     1021 gcccgaaccc gtgccgattt ccaaaaccgt atcgtttttc gtcagcttca agccctgcgc
     1081 cagccgcgcc acgactttcg gctcgagcat cttatgaccg ttggcaagcg gcagcgccat
     1141 atccgcatac gccaaaccct gcaagtcctc atcgacaaaa agctcgcgcg gaatctccgc
     1201 caaagcgtcc aaaacgtcaa aatccaatac atcccaagga cggatttgct gttcgaccat
     1261 attgaaccgc gctttttcaa aatccattta gtgctccgtc aaataattgt tccaacaata
     1321 gcaggcatta taaaaccaca ctccccccgc gccaacttcg gcacgcgccg cccgcccctt
     1381 tccatcactc aagccgcctg gcgcgaagcc tcaaaccgct tctccaaaat cttcgccagt
     1441 tcgcccaaat acaaagcatc tgtctcatca aactgcgcca aatgttcgct gtccgcgtcc
     1501 aacacgccga tacagcggcc gtctgaaaac agcggcacga caatctccga acgcgacaaa
     1561 gacgaacagg caatatggtc gggatgcgcg tccacatccc cgacgaccac cgtcccgccc
     1621 ttcgcccaag cctgaccgca caccccgcga ccgaacggaa tccgcgtaca cgccaaaggc
     1681 ccctgaaacg gtgccaaaac caattcgtcc gaacgcgtat cgaccaaata aaaacccacc
     1741 caaaaccaac cgaacgcctc cttcaaaacc gccgccgtgt tcgccaaatt cgccacccaa
     1801 tccgtctcgt ccgccaccac agactcaatc tgcggcaaca cctcccgata aagcgcggcc
     1861 ttgtccgaag ccgaaaaatg aagcgcgtgc atcacatctc ctatagttgc atacatatca
     1921 ggcggccatt ataaaacaac ctgcccgaaa caacattcca aaccgccccc gcagccgccc
     1981 gccccaagtt gcgaacccgc cgcatatcca ctaaacttca cattgcaacg cgccacacgc
     2041 ggcagacaga aaaaacacga cacggagcaa aaaagatgta tcaccaaatc ggaatgtggg
     2101 atcaaaaatg ggtcatcggc aactggaaaa tgaacggccg gctccagaac aacaattcgc
     2161 tcatgcaccg cttccgcatc ctccccaccg ccgaacgcgt cctcatcgga ctcgccgccc
     2221 cgaccgttta cctgctgcaa ctgcacaacg ccatgcaaat cgtcttaaac aaccgcatcc
     2281 tcacctgcgc ccaagacgtg agccgcttcc ccgacaacgg cgcgtacacc ggcgaagtgt
     2341 ccgccgaaat gctcgccgac accggcacag acatcgtcct catcggacac tccgaacgca
     2401 gcctttattt cggcgaaaaa aacgaaatcc aacgccgcaa aatggaaaac gtcctcaacg
     2461 tcggactcat cccgttattg tgcgtcggcg aaagcctcga agagcgcgaa gccggcaaag
     2521 aacacgaagt catcgcccat cagctttcca tcctgcaagg gctggatacc aaaaacatcg
     2581 ccgtcgccta cgaacccgtc tgggcgatcg gcaccggcaa agtcgccacc gtcgaacaga
     2641 ttgccgatat gcacgcattc atctacaaag aaatcttgtc tttgtgcgga agcgatgtta
     2701 aaatccgcgt cctttacggc ggaagtgtga aagcggacaa cgcggccgac atcttcgcag
     2761 taccttatgt ggacggcgca ctcgtcggcg gcgcgtcatt gtcgtacgac tcctttaccg
     2821 ccatcatcag tgccgcacaa aatgcgtaga aaaatatgga agccttcaaa accctaattt
     2881 ggattgttaa tataatttcc gctttggccg tcatcgtgtt agtattgctc caacacggca
     2941 aaggcgcgga tgccggcgcg actttcggat cgggaagcgg cagcgcgcaa ggcgtattcg
     3001 gctctgccgg caacgctaac ttcctcagcc gttcgaccgc cgttgcagca acatttttct
     3061 ttgcaacctg catggctatg gtgtatattc acacccacac gacaaaacac ggtttggact
     3121 tcagcaacgt acaacaaact cagcaagcac ccaaacccgt aagcaatacc gaaccttctg
     3181 cccctgttcc tcagcagcag aaataacagt ttttcaaatg ccgacatggt gaaattggta
     3241 gacacgctat cttgaggggg tagtggccgt aggctgtgcg agttcaaatc tcgctgtcgg
     3301 caccaacaca caaaaacgcc tgaaaatttc aggcgttttt tgttcaatcc catccaacgc
     3361 tgtcaaccaa ttccaatcaa tacaaggatt tcaggctgat tgttatcctg ccgtccccct
     3421 tcctgacagt gcaatcccgt ccaatccgcc ctaattgaag taacctaaaa tttacggtat
     3481 cttttgcggt atctgaaaaa tacctcgaaa aaataccgca aaaataaagc tgaacgaccg
     3541 ccaaatcagg aatgccaagc ggaaaagagc ttgcggggaa tactgccaag acgtagggaa
     3601 caagggggaa accgtccaag atgcagggcg gttttttttg ggtttttgga aaaaacctat
     3661 actaggaagc gataccctta gttgttacct tgttaccggg gaaaagttag ataaataagc
     3721 atatgaaata tagtgaatta aatttaaatc aggacaaggc ggcgagccgc agacagtaca
     3781 aatagtacgg caaggcgagc caacgccgta ccggtttaaa tttaattcac tataaaataa
     3841 gaaaaagata aaaaattggt aacaaatgcg gtaacaaatg gtaacgaatc ggtaacaact
     3901 tttggggttt tccggttttt caccgtcttg gcagtgggag cgtagcggaa tgaaaagcca
     3961 aaacgcacgg aaccgcgcct attttgagca ggaatggcgg ttaaaccact tggttatata
     4021 tggggacata ggaagacagc gaaacgcgct aaaaacgcaa tttgagccat taaaagcgat
     4081 tgattaaaaa ataatcaggt tagccgccag tgtttcaggc ggcataaacg gagaaattgc
     4141 ggggcataaa aaaggcagct tgccgtgttg tctgtctctg gtataattcc aagtat
//
LOCUS       DAXHKX010000165         4192 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHKX010000165 DAXHKX010000000
VERSION     DAXHKX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.165"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..315)
                     /locus_tag="ACFMWZ_001816"
     CDS             complement(<1..315)
                     /locus_tag="ACFMWZ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC5935733.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLPARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARS"
     gene            511..1237
                     /locus_tag="ACFMWZ_001817"
                     /pseudo
     CDS             511..1237
                     /locus_tag="ACFMWZ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            1306..2844
                     /gene="lnt"
                     /locus_tag="ACFMWZ_001818"
     CDS             1306..2844
                     /gene="lnt"
                     /locus_tag="ACFMWZ_001818"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC5935734.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILVKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3108..3971
                     /gene="rpoH"
                     /locus_tag="ACFMWZ_001819"
     CDS             3108..3971
                     /gene="rpoH"
                     /locus_tag="ACFMWZ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC5935735.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
BASE COUNT         1028 a         1299 c          959 g          906 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttcg attcggctat acgccttttc acgcaggctg taattgaatt tccacaaacc
      121 gccgaacact gccggcggac gcgcgaccag catcaggaac gccgcaatcg ccaaggcttc
      181 cgccgccccg tccaacacgc cggcgggaga aaccagcagc aaaggcattc ccaaacgtgc
      241 gggtaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca ggaacagact
      301 gcccatccaa tccacgctaa cgattctccc ttaaaattca ttaaattaaa atcgaaagac
      361 tgtaacacgg cattttcttt tcacgaaact ttacttcacg tccgccacag cgttcagacg
      421 gcattttcct gtttcatgcc gcctgaaacc gcccaaatcc gcgttctcct atataatgtc
      481 ggactttgcg ccgcatttca ggattcttta atggcatttg cctcactatt taccctgccg
      541 gacgacatca ccgccgtttt ggacgatgtt gccctgatga ccaaagccgc cgcaaagaaa
      601 accgccggcg tggtcggcga cgatttggca cccaatgcca accgtgttac gggcgtatcg
      661 gcagaacgcg agttgccgat tgtttggtcg atggcgaaag gttctttttc caacaagccg
      721 gttttggtgc ctgcggccct gctgctttcc gccttcctgc cgcaactcat tacgccgctt
      781 ttgacggcag gcggcattta tctgtgcttt gaaggcgtag aaaagctgct gcacaagttt
      841 ctgcaccgac atgacgcgca tgctcgtaat ggcggcaatc ggcatcggca tgactgtttt
      901 ggtgtacgga atcgttgccg tcatcgtcaa actcgacgac ttaggcatgc tgctgatgcg
      961 ccgcccacaa accttttcgc gcagtttggg gcaaatgctg actgccttta tgccttgctt
     1021 tatgcgcggt ttgagcgtgg tcggcacgct tgccatgttt cttatcggcg gcgtactggt
     1081 cgcccacaac ctcggccttc tgcacgattt cctgcacgcg caacactggg acgctggctg
     1141 ggcggagtac ttcgccaacc tcgttgtcgg gctgctttcc ggttcgattg cctgcgcccc
     1201 cgccttgccg ctgatgaatc gtttcggcag gcattgattt ctttttcaaa taccgatgcc
     1261 gtttgaaaga tgttcagacg gtatcttccg aacagacaga tgaatatggt ttccaaactg
     1321 gacaaatact ggcagcaccc cgccctctac tggcctttgc tcatcctttt tgccgccgcc
     1381 acccccttta ccttcgcacc ctactaccac ttttggctga tgcccttgat tttcggtgcc
     1441 ttcgtccgcc tcatcgaact gcgtccgcgt tttgctgtct cttccgccta cctgttcggc
     1501 ctgaccgcat acacgacaca gttctactgg atacacaccg ccctgcacga cgtttccggc
     1561 ctgcccgacc tctatgccgt accgctgacc ttcctactcc ccgcctacct tgccctttat
     1621 ccggcactgt gtttctggct gtggaaaaaa tttaccctgc ctcggggcat aaaaatcggt
     1681 ttggtactgc ccatcctgtg gacactgacc gagtttgccc gcgaacgttt cctgaccgga
     1741 ttcggctggg gcgcaatcgg ctactcccaa atcaccccgg acagcccgct cgccggcttt
     1801 gccccattgg gcggcatcca catggttaca ctggcaaccg cctttctcgg tgtctggctg
     1861 gttttggcga gtgataacac cgcacgttcg ggcaaacgcc tgcttccaat tatcctgatt
     1921 gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg ccccgacggc
     1981 agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa atggcgtgaa
     2041 gaccaagtca tcccgaccat acagaaatat tacgaacagg tcggcaaaac cactgccgac
     2101 atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc ggaaaacata
     2161 ctggtaaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt cggcatcagc
     2221 caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac cggttatcag
     2281 gaaaacaatc aggacggcat cccctactac gccaaaaacc acctcgtccc cttcggcgaa
     2341 tacaaaccgc tgcccttcct gaccacgccg ctttacaaaa tgatgaatat gcccctttcc
     2401 gacttccgca aaggcggcgg taagcaatcc gccctgctga tgaaaaacca aaaaatcgcc
     2461 ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc caaagatgcc
     2521 acactgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc catgtaccag
     2581 cacctccaac aatcgcaggc acgagctatg gaactcggac gctatatggt ccgtgccacc
     2641 aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca agcccaaccc
     2701 gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac gccctatatg
     2761 aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact gattcttttc
     2821 atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccggacgggc tgtctttttt
     2881 caaaacggct atttacatac aattacgtat gtaatataat tctgaaatcg aattgagcca
     2941 tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt caaaccttag
     3001 cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact gcatcccaac
     3061 acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg aataacgctt
     3121 tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc cacaccgtca
     3181 acagcatccc gatgctgtcc caggaagaag aaacccgcct cgccgaacgc cggataaagg
     3241 gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc gtttccatcg
     3301 cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa ggcaatatcg
     3361 gactgatgaa ggcggtcaaa cgctacgagc ccggtagagg cgcgcgtctg ttttcatttg
     3421 ccgtacactg gattaaagcc gaaatccacg agtttatttt gagaaactgg cgtttggttc
     3481 gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt atgcgtaaaa
     3541 acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg ggtgtcaaac
     3601 tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc atcatggcag
     3661 acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac cacgattccg
     3721 aaccaagccg acaactatcg aaacaggcgc attacgccct gcaaacagaa ggtttacaaa
     3781 acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc tggcttcaag
     3841 acgacggcgg gctgacgctg caccagcttg ccgccgaata cggcgtctct gccgaacgca
     3901 tccgccaaat cgaagcaaaa gccatgcaga aactgcgtgg tttcctgacc gaagaagccg
     3961 aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg gtattttatt
     4021 tggtaaacat caatgcggtt tgtgcgtcgt gccgtacacg attttatccg tccaagccat
     4081 cgcaatcgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca gctgcttttc
     4141 cgacagattg gcgaaattga tatagtggat taacaaaaat caggacaagg cg
//
LOCUS       DAXHKX010000166         4162 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHKX010000166 DAXHKX010000000
VERSION     DAXHKX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.166"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(92..943)
                     /locus_tag="ACFMWZ_001820"
     CDS             complement(92..943)
                     /locus_tag="ACFMWZ_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC5935736.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1291..2805
                     /gene="katA"
                     /locus_tag="ACFMWZ_001821"
     CDS             1291..2805
                     /gene="katA"
                     /locus_tag="ACFMWZ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC5935737.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2881..3102
                     /locus_tag="ACFMWZ_001822"
     CDS             2881..3102
                     /locus_tag="ACFMWZ_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935738.1"
                     /translation="MPSETSFDRFRRHFYTPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRESGNLERKI"
     gene            3559..4125
                     /locus_tag="ACFMWZ_001823"
     CDS             3559..4125
                     /locus_tag="ACFMWZ_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC5935739.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMLREGLSGGADDVREAKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GKRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          964 a         1116 c         1124 g          958 t
ORIGIN      
        1 gatgcaggtt tccgcgcgga cggattcggc attcccgctt tcgcagaaat gccggctttt
       61 ggtttgttgt ctttataaga ttattcggca attattctgc ggttttgcgt ttgtgtgctg
      121 ccggaatctc aaaggattct ctgtatttgg caatggtgcg gcgagaaacc tccatgccgc
      181 ggaaagccag caggttggcg agcgcctcgt cagaatacgg cgtgggtgtg gacgaggcaa
      241 aaccagcggc gggaaaggga ttgagcgaag caatgaggtc gagtgcggct tcgagtgtgc
      301 cgctgtcggt ttggggcagg cgttttttta ttcgcgcgag ggtttggctg cggttgccgt
      361 caatgctgtc gagggcgttt cggacgatat gcagggcgga gggtttggca gcacattcgc
      421 ccgatctttc tatctgcagt atcagcgatt cgttcaaatc ggcggcggcc acgcctgccg
      481 ggtcgaattt tttcaatgcg gtcagggctt gtttcagcat tgcttcatcc aacatccact
      541 ccaagggcgt atggtcgagg atgtcttcga tgctgtcggt cagataacct tgctcgtcaa
      601 ggaaatcgat gaggatgtgg acacaggcgg attcttggtc ggaaagcggg tgttcgcata
      661 cttgcgcgtg caggtattgc ttgaaatcct gctcgccggc gatgttggac agcatatctt
      721 cgccttcgtc tccgccgatt tgacgggcag gcgcagtgta atggctgaac tcggcatcgg
      781 aaaattcatc cgtgtctttg cgttcgagca gggggttgtc cgacagccag ttttcgacct
      841 cgcgttcaag ttcgataccc gacatctgca atacgcgcaa agattgttgc agccgctggt
      901 tgagctgctg ggtctgtttg agctttattc cgagtaaggt catgataatg tggggaaaat
      961 tgttattttc agcctgtcgg cgcaaaaaat gccgcaaagc gtcattgcat tataaatggt
     1021 tttaatgagc gggttcggat tccgttcgat aacaaaaaac aaacgaaaat caagaaccga
     1081 ttgcttataa taatattaaa tcgatttcat agttttaata gcgaaaatct tggcgtatag
     1141 tcgcatccat agtttttaca aaagggaaat aaaatgtcga ttcaagaaat ttattgcaat
     1201 caagaaaccg gttacgaata cgctttccgc caaatcgtac tgtaagccgt ttacccccat
     1261 ttgaccaacc tgagaaaagg aacaagagcg atgactacct ccaaatgccc tgtaacccat
     1321 ctgaccatga acaacggcgc gcctgttgcc gacaatcaaa acagcctgac cgccggtcct
     1381 cgcggccctc tgctggcgca ggatttgtgg ctgaatgaaa aactcgccga cttcgtgcgc
     1441 gaagtcatcc ccgaacgccg tatgcacgcc aaaggttcgg gcgcgttcgg tacgtttacc
     1501 gtaacgcacg acatcaccaa atacacccgc gccaaaatct tcagcgaagt cggcaaaaaa
     1561 accgagatgt tcgcccgttt caccaccgtg gcaggcgaac gcggcgcagc cgatgcagaa
     1621 cgcgacatcc gcggttttgc cttgaaattt tataccgaag aaggcaactg ggatgtggtc
     1681 ggcaacaaca cgcccgtgtt cttcctgcgc gacccgcgta agttccccga cctgaacaaa
     1741 gccgtcaaac gcgacccgcg caccaatatg cgctctgcca caaacaactg ggacttctgg
     1801 acgctgctgc ccgaagcact gcaccaagtt accatcgtga tgagcgaccg cggcatcccc
     1861 gccggctacc gccatatgca cggcttcggt tcgcatacct acagcttctg gaacgaagca
     1921 ggcgagcgtt tttgggtgaa attccatttc cgcacccaac aaggcattaa aaacctgacc
     1981 aacgaagaag ccgccaaaat catcgccgac gaccgcgaaa gccatcagcg cgacttatac
     2041 gaagccatcg aacgcggcga gtttccgaaa tggacgatgt acatccaagt catgcctgaa
     2101 gcagacgcgg aaaaagtacc ttatcatccg tttgacctga ccaaagtttg gccgaaaaaa
     2161 gactatccgc tgattgaagt gggcgaattc gagttgaacc gcaatcccga aaacttcttc
     2221 gccgatgtgg aacaatccgc cttcgcaccg agcaacctcg ttcccggtgt cggcgccagc
     2281 ccagataaaa tgttgcaggc gcgtttgttc aattacgccg acgcgcaacg ctaccgcttg
     2341 ggcgtgaatt tccgccaaat ccctgtcaac cgtccgcgtt gccctgttca cagcaaccag
     2401 cgcgacggac aaggacgcgc cgacggcaac tacggcagcc tgccgcacta cgaacccaac
     2461 agcttcggcc aatggcagca gcagcccgac ttcgccgaac cgcctttgaa aatcaacggc
     2521 gacgcggcgc actgggacta ccgccaagac gatgacgact atttcagcca accgcgcgcc
     2581 ctgttcaacc tgatgaacga cgcgcagaaa caggcattgt tcggcaacac cgccgccgca
     2641 atgggcgacg cgcccgactt catcaaatac cgccatatcc gcaactgcta ccgttgcgat
     2701 ccggcatacg gcgaaggcgt ggcaaaagcc ctcggactga ctgtcgaaga cgcacaagcc
     2761 gcccgcgcga ccgaccccgc actcggccag cccggtttgc tgtaaggggg cattatgtgg
     2821 atggaaattg aagaaatcct gtcccgcgcc gtccgctacc ggaaataacc gggcataaaa
     2881 atgccgtctg aaacgtcgtt tgaccgcttc agacggcatt tctacacccc gcccccgccg
     2941 tttcagtggg cgttttttat taaacgcaaa atatcccgtc attcccgccc cacagcccac
     3001 ttttccgccg tcattcccgc accatcccca ccggttcaaa ccggcacgaa aacttttccg
     3061 cgtcattccc gcgaaagcgg gaatctagaa cgtaaaatct aaagaaaccg tttttcccga
     3121 taagtttctg caccgacaac tctggattcc cgcctgcgcg ggaatgacga atccatccgc
     3181 acggaaacca aaatcccgtc attcccgcga aagcgggaat ctagaacgta aaatctaaag
     3241 aaaccgtttt atccaataag tttctgcacc gacaactttg gattcccgcc tgcgcgggaa
     3301 tgacgaatcc atccgcacgg aaacctacat cccgtcattc ccacgaaagt gggaatctag
     3361 aacgtaaaat ctaaagaaac cgtttttccc gataagtttc cgcaccgaca actctggatt
     3421 cccgcctgcg cgggaatgac gggatttctg tttttgattt tttgtttttg caggaatgac
     3481 gggatgtggg ttttcgtgcg gcgtttttgc atttttttgc ttttgctata atccgccctt
     3541 tttgaggacg ggtgcggtat gggtttttat gctttgctct tgattgcgct ggggatgtcg
     3601 atggatgcgt ttgccgtcgc attggcaaag ggtgcggcgg tcagaatgcc tccgcgcaaa
     3661 attgcggcaa cggctttggt gttcggcacg gttgaagcgc tcacgccgct ggcaggctgg
     3721 gtgggcggtt tttatgccaa gccgtttatc agcgaatggg accattgggc ggctttcgtc
     3781 ctgctgggcg ggctgggtct gaagatgttg cgtgagggct tgtccggcgg ggcggacgat
     3841 gtgcgcgaag ccaaacggga aagctggtgg ttgacggttc tgactgcttt tggaaccagt
     3901 attgattcca tgatagtcgg ggtgggcttg gcgtttatgg aggtaaacat cgcctttgcc
     3961 gccgccgtca tcggcatggc gacgacggtg atggttacgg tcgggctgac ggcgggaagg
     4021 gctttgggcg tattgttcgg caaacgcgcc gagtttgccg gaggtttggt gttgattgcc
     4081 atcggtacat ggacgctctt gtcgcatttg ggtttgattc aatgatgtcg gaaaatatag
     4141 tggattaaca aaaaccagta cg
//
LOCUS       DAXHKX010000167         4078 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHKX010000167 DAXHKX010000000
VERSION     DAXHKX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.167"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(184..1377)
                     /locus_tag="ACFMWZ_001824"
     CDS             complement(184..1377)
                     /locus_tag="ACFMWZ_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC5935740.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGVCSMKNWLNQGWAAKDGVHFSAKGYQRSAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1374..2360)
                     /gene="patB"
                     /locus_tag="ACFMWZ_001825"
     CDS             complement(1374..2360)
                     /gene="patB"
                     /locus_tag="ACFMWZ_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC5935741.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTLSDGIKAFLSGETPPTPQDGGSADMPPEAAASEAAPQTGEI
                     QEWKQDTDTAAVRSGDKVFFVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYP
                     SFFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDR
                     ILEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGKD
                     RYTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2371..3807)
                     /locus_tag="ACFMWZ_001826"
     CDS             complement(2371..3807)
                     /locus_tag="ACFMWZ_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC5935742.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPMI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(3935..>4078)
                     /locus_tag="ACFMWZ_001827"
     CDS             complement(3935..>4078)
                     /locus_tag="ACFMWZ_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFC5935743.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IRT"
BASE COUNT          836 a         1031 c         1332 g          879 t
ORIGIN      
        1 ggaaggcggc agtttatcat ctttgccttc agacggcata tcgaacgccg cctttgcgcc
       61 gtatgccgga ggttcgcctg cgtctttcca atgtctgagg cgaatgccgt ctgaagcaat
      121 atgaaggtat gcgtttcaga cggcatcccc ctgttgcgga aataccgtcc gcctcctgtc
      181 cgattattgc ctgattgcag cggcgcggac gagttcttcg aggctgtcgg cgagcatttc
      241 cgccgaccgt tggtagcctt tggcggaaaa gtgtacgccg tctttggcgg cccatccttg
      301 gttgagccag tttttcatgc tgcaaacgcc gcccatcgcg ttttgccaag accagaacat
      361 cgtctgcccc tgacgggcga tgcgccgctg catctgttgg acttcggtca ggcggacggg
      421 gcgtgtgccg catacgccga gcgtgttttt cagggattcg ggtgcgccga tgatgaggat
      481 gccggcggca ggcaggctgt cgcggatttg gcggacggta tccagccatt tttgttcagt
      541 gtcggcaatg tcgatgttgt cgccgaaggc ttcgttggtg ccgtaggaaa ggataaccaa
      601 atcggcgccg gtttgggcga ggtcgttcat acggtcggca cgccatttcg accactgggt
      661 caattgtgcg ccgttgatgc ccatcgcgga aacggtaatg ccgccggcgg gattttcgat
      721 gttgatgaag ccgatgtccc acggcatttc ggtgtgtatg gtcaggggca gtgccgcgcc
      781 cgtatccagt acctgccagc cgccgccgtt ggcggagacg gtgttgccgt tgacggtcag
      841 ggtttgttcg gcgagcaggg gtttggcaaa cagggaaatg cgctgtttgc cggtcttgcc
      901 gtccgatgcg gtcagggtca tgctgccgcc gctgccggtg tgggcgagga tgccgccgag
      961 cgggaagtct ccgctattgt tcctgctggt gaggctttgc cagttaccgt tgtgccggac
     1021 ggccgccatg cgctgccctt tgacgttggc ggggtaaacc cagcctatgc cgccgtcgcc
     1081 ccaagttttt tgcaggcgtt tgcgcaggct gtcggtaaag aagtcgccgg cggtatgcga
     1141 gtcgccgatt tgcaggatgc ggaaggtctc gccgctgcct tgtgcgacgg atcggagttt
     1201 tttcatccaa ggcgaggcgg cggcgttgcc gtagtcggtc aagagcccgt ttgtatcggt
     1261 gtagggggcg gacggggaaa cggtaacggt gtcgaggctg acggaggcga cgggcagggc
     1321 ttctgcctgc gtggcggcga acagggcgga aaatgcgatg aggtgtttgg ggttcatggc
     1381 tgtgtacttg atggttgcgt acttggttca aaaacgattt tttccattat ttttgccgcc
     1441 agcagttttt gtccttcggc ggtaaagtgt atgccgtcct tgctgcggta gcggacgggt
     1501 ttgccgttga cgttgacgga gtccgtgtag cggtctttcc cgccgctcag ggtgtgcgcg
     1561 gtgggaatca ggatgatttt gcctttcaaa tgttccgaaa gcagtttgtc gaggtagcgc
     1621 atctgcccgt cgagcttggc ttttttcatg taggggatgc cgagccagac gacttggacg
     1681 cggtgcgtgt gtgcggcttc aaggatgcgg tcgacgcgtt tcaggtattc ttgcgcccat
     1741 tcgtcggaag cgaatttgag gtagcgtttg ccgacgggga aatcccacgg gtcgttcggg
     1801 ccgaggaaga cggcgagcac gctgatttcg ggatgttttt gcagggtttc ttcaatcgtt
     1861 ttcggccagt cgaagaatga ggggtaggac agccccgtgc tttgtttgct gaggttgacg
     1921 gattcgatgc cgtattgctg tttcaggctt ttttgcacga agggggcaac gccctgcatc
     1981 agcgagtcgc cgacaaaaaa gactttgtcg ccgctgcgga cggcggcggt gtcggtgtcc
     2041 tgtttccatt cttggatttc gccggtttga ggggcggctt cggatgcggc ggcttcaggc
     2101 ggcatatctg ccgaaccgcc gtcttggggc gtcggcggcg tttcgccgga caggaaggct
     2161 ttgatgccgt ctgaaagggt gtaggcgttt tcttgcaacg ccgcaccgct ccgccaccat
     2221 ccgtaggcgg caagcggttc gagcggactg ttgcggtggt aggtctgctg ccagtaggcg
     2281 ttgatggggt tttggctgaa ccacacggca atcagggcag acatcagtat ggaggcgaaa
     2341 agggaaagaa agtttttcat gtccaaaccc ttaaaaattg gcataaataa agccgggtat
     2401 ccccgagggg gccaatacga taatcagcag caggacggcg gaaacgggga taaaccacag
     2461 ccacatcggg attttttcca aacctttgac cgcgccgtcg aaagcgcgtt gcaggtaagg
     2521 gtagagcagc atcacggatg caaacgaggc aagcagcagc atgtttgcct gttgcggcgc
     2581 attccagccg ttggcattgg caaagagggc attgaaaact gcgcctgcat cgtcggggct
     2641 tgcggtattg aagacaacaa agctaaggca gacgaaatgg aaggtaacaa accacgagag
     2701 cggcgcaagg tatttcagac ggcatagcgc gttgcgtccg aaatagcgat cgcccgtgtt
     2761 gagcagcacc agcgccgtgc cgtgcagcgc gccccaaatg aggaagttcc agccgtagcc
     2821 gtgccagatg cctgagagca ccattgccgc catcaggttg aactgtgtcc gcaaaaagcc
     2881 ttttttgctg ccgcccaagg ggatgtagat gtagtcgcgt atccaggtgg aaaggctgat
     2941 gtgccatttg tcccaaaatg cgcggatatt cgcggcacga agcggtgcgg agaagttttt
     3001 gggcagccgg aagcccagca gcattgccat tccgataacc aagtcggaat atccggaaaa
     3061 gtctaaaaag agttggaagg tatagccgta cacgcccgcc aatacgcccc agccgtcgaa
     3121 ttgggcggga ttttcaaata cgggcgatac ccagttttct gcgagcatcc ccgccagcca
     3181 ccattttttg gcaataccca gcagaatcag ggaaacggcg agtgcggggc ggacgggtga
     3241 gcgtggttgg tgggtacgga tttgcgccaa tgcgcccgcc tgctcgccgt ctgcgctttt
     3301 gaatgcggcg gcgcggataa tcgggccgga ggtaacggtg gggaaaaaac tcaggtgcag
     3361 cagcagctcg tgccactcga aacgcgcggc gtgcggggcg cggaagcagt aaaccagata
     3421 ggcgagcgac tggaaggtgt aataggaaag ccccagcggc atcaggatgt cgattgcgcc
     3481 gccttttccg gcatattggg caatcatcgg gcggaaaaag tcgaaatatt tgaaaaagcc
     3541 caagacggtc agcgaggcgg caatgccgca ccccagccag aaacggcgcg tattttcgcg
     3601 atcggaacgg agcagttcgc ccaaaaggta cacgcaggag gaataaagga cgatgattgc
     3661 cgcaaatacg gggccgatat ggtagagcca gcccataccg gcagccaaaa gcagcaggtt
     3721 ttggacggac gggtatttcg ccaagcccca gtaaatcggc aggaaggcga gaaagaacag
     3781 tgcgaactcg acagacagca gcggcataaa ccatccttgg aacaaaaaat aggcgttaaa
     3841 acacggcaaa gcccgttatt ttaagcaggg gtggatattt cttcaatcaa aatgccgtct
     3901 gaagggcggg agaggcttta gacggcatag cgggtcaggt tcggatatgg cggttcaagt
     3961 tcaggcacag ccacagcccg gccgccgccg caagcatacc ggcgatgccg atacgggaaa
     4021 taccgacata ttgcgttacc caatgtccca acagtgcgcc gctgccgata ccgacgtt
//
LOCUS       DAXHKX010000168         4060 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHKX010000168 DAXHKX010000000
VERSION     DAXHKX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.168"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            38..461
                     /locus_tag="ACFMWZ_001828"
                     /pseudo
     CDS             38..461
                     /locus_tag="ACFMWZ_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="ACFMWZ_001829"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="ACFMWZ_001829"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC5935744.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="ACFMWZ_001830"
     tRNA            1210..1285
                     /locus_tag="ACFMWZ_001830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="ACFMWZ_001831"
     tRNA            1311..1386
                     /locus_tag="ACFMWZ_001831"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="ACFMWZ_001832"
     tRNA            1410..1485
                     /locus_tag="ACFMWZ_001832"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="ACFMWZ_001833"
     tRNA            1507..1582
                     /locus_tag="ACFMWZ_001833"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1608..1681
                     /locus_tag="ACFMWZ_001834"
     tRNA            1608..1681
                     /locus_tag="ACFMWZ_001834"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Cys,seq:gca)
     gene            1784..1873
                     /locus_tag="ACFMWZ_001835"
     tRNA            1784..1873
                     /locus_tag="ACFMWZ_001835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Leu,seq:taa)
     gene            1977..3029
                     /gene="sohB"
                     /locus_tag="ACFMWZ_001836"
     CDS             1977..3029
                     /gene="sohB"
                     /locus_tag="ACFMWZ_001836"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC5935745.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3080..3940)
                     /locus_tag="ACFMWZ_001837"
                     /pseudo
     CDS             complement(3080..3940)
                     /locus_tag="ACFMWZ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(3951..>4060)
                     /locus_tag="ACFMWZ_001838"
     CDS             complement(3951..>4060)
                     /locus_tag="ACFMWZ_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC5935746.1"
                     /translation="HKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1024 a          962 c         1059 g         1015 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattcgaa tatgcggaat tgaccgttac
      121 cgcccccaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttctg cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccctatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgccgccg tcatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacagacttc cgatttcggc gccttcctcg
      781 atcccgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatta tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 tcaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaatg tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgttttcc
     1381 gctccaagtt ttatttcaaa catatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgcg agttcgagac tcgttttccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagtttttt agataagcca gttttagggc gagatagcaa
     1621 agtggttatg cagcggattg caaatccgtc tacgccggtt cgattccgac tctcgcctcc
     1681 attatcgtac tacggcgggg tggcagagtg tttatgctat gaggagtgca atcttcatat
     1741 aggccggtta aaatccgcgc ccccgcctcc acctttcaca aatgcccggg tggtgaaata
     1801 ggtagacaca acggacttaa aatccgtcgg gactaaacat cccgtgccgg ttcgattccg
     1861 gctccgggca ccaagctgaa aatgacaatg ccgctccaag cggttatttt tttatctgtc
     1921 ggacggtgat ggacaatatt gacatgttca tgcctgaaca agaggaaatc caatcaatgt
     1981 ggaaagaaat tttactgaat tacggtattt tcctgctcga actgcttacc gtgttcggcg
     2041 caattgcgct gattgtgttg gctatcgtac agagtaagaa acagtcggaa agcggcagtg
     2101 tcgtactgac ggatttttcg gaaaattata aaaaacagcg gcaatcgttt gaagcattct
     2161 ttttaagcgg ggaagaggca aaacatcagg aaaaagagga aaagaaaaag gaaaaggcgg
     2221 aagccaaagc agagaaaaag cgtttgaagg agggtgggga gaaatcttcc gaaacgcaaa
     2281 aatcccgcct ttttgtgttg gattttgacg gcgatttgta tgcacacgcc gtagaatcct
     2341 tgcgtcatga gattacggcg gtgcttttga ttgccaagcc tgaagatgag gttctgctta
     2401 gattggaaag tccgggcggc gtggttcacg gttacggttt ggcggcttcg cagcttaggc
     2461 gtttgcgcga acgcaatatt ccgctgaccg tcgccgtcga taaggtggcg gcgagcggtg
     2521 gttatatgat ggcgtgtgtg gcggataaaa ttgtttccgc tccgtttgcg attgtcggtt
     2581 cggtgggtgt tgtagcggaa gtaccgaata tccaccgcct gttgaaaaaa catgatattg
     2641 atgtggatgt gatgacggcg ggcgaattta agcgcacggt tacttttatg ggtgaaaata
     2701 cggaaaaggg caaacagaaa ttccgacagg aactggagga aacgcatcag ttgttcaagc
     2761 agtttgtcag cgagaaccgc cctcaattgg atattgagga agtggcaacg ggcgagcatt
     2821 ggttcggtcg gcaggcgttg gcgttgaact tgattgacga gatttcgacc agtgatgatt
     2881 tgttgttgaa agcgtttgaa aacaaacagg ttatcgaagt gaaatatcag gagaagcaaa
     2941 gcctgatcca gcgcattggt ttgcaggcgg aagcttctgt tgaaaagttg tttgccaaac
     3001 ttgtcaaccg gcgggcggat gtgatgtagt ttgccgaacg gatggaaatg ccgtctgaag
     3061 cgtgttcaga cggcattttt caagttccgg ctttgatgtt ccgtttcgac cactctatcc
     3121 gttgtccgat gtctttcaaa ttggaatatt gttcgaccaa tcgcggcacg tcgtccaaac
     3181 tcatggcaaa catccacaga taagtgccga ccgaatagac atgccccgcg ctgctgtagc
     3241 ctttgagcgt catcatcaca aaagcaaagc cgaacaaaat acccatcgcc gtgccgacgc
     3301 agagatagcc gaaggcttcg cggttggaaa tcagcacacg caggcgcgca agcagtccgt
     3361 aatggcggtc cagctgccgc tcgtcgcctt ttcggataaa gtggttgtcg cgttccaagc
     3421 tgttcttcag gcggaaatac aggttttcgc tgatggcggc aaaacgtggc aaaagccata
     3481 aaaacaacgc aagtatgccc accgccgaca cgccgaccca aaattccagc accagcagca
     3541 tgatgcacgc gccgaatatg gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa
     3601 aaaagctgac aaactcacgc gacagggcaa cccgcgcagt taccgccgaa tgcgggactt
     3661 gccgctgccg ctgttccaac acaaccggca cggcgatttc ggtataaatc cgcgtaaacg
     3721 tgcgcgtatc ggcaatccgc cgcaccgacc agccacatca aaagcacaac caaagcgtac
     3781 agcaacgcct gccacgcctg ccccgcaatc acggcattaa tcgcccagcc gccaaacacc
     3841 ggatacacca gcatcaaaag gttttccagt ccgaccagaa aaaatgtgcc aatcaatcgc
     3901 ttgcggtggt tttggctatg tgtttcaaca ttttccacat atttgccccc ctatttttta
     3961 ttttccgaga ttgttttttc catcacttca gccagtgcat ccaaatcggc aaacagccgg
     4021 gttgtgcgct tgtcgccgaa tgtggcaaat actttgtgag
//
LOCUS       DAXHKX010000169         4052 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHKX010000169 DAXHKX010000000
VERSION     DAXHKX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.169"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(173..484)
                     /locus_tag="ACFMWZ_001839"
     CDS             complement(173..484)
                     /locus_tag="ACFMWZ_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC5935747.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFHSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            1013..3292
                     /gene="nrdA"
                     /locus_tag="ACFMWZ_001840"
     CDS             1013..3292
                     /gene="nrdA"
                     /locus_tag="ACFMWZ_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC5935748.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            3313..3885
                     /locus_tag="ACFMWZ_001841"
     CDS             3313..3885
                     /locus_tag="ACFMWZ_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935749.1"
                     /translation="MKAFPKFDYQGIEYDLTHLTAFSCDYIQPAKSDGTAEKRYRCII
                     EFSTHCFTRGENKRKGETLSDIEPALQYITAKETRIFCFERYQVSKMLPQIMREISRN
                     KCYFTSADDKFLTISVTDKNGKKVDYEIYFSLQRAKSPKHDVHIYINSAYIRDGDYKE
                     NHGTKVRRKPVGFFVLLHNTLVNKRIKRPK"
BASE COUNT         1123 a          975 c          956 g          998 t
ORIGIN      
        1 cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
       61 attaaggctg cggcaacggg aggacctcaa acgaaatggc gtaaggatag tttgttgtat
      121 ctatggaaaa tgccgtctga acctgtgttc agacggcata ttttatatgg gattaacggc
      181 ggaaactgcc gccgtcgaaa tcatcgaaag cctcataagc cgcccggcat tggattttct
      241 gttccgcata aggcagggag tggaatgctt tgcgcgcctt tttgttttcg cgcaattcct
      301 gcacgttgcc ttgcgatcgg taccacgcgg cacggcgttc caaatatttc ctgcattcgg
      361 ggtgcagcat ctgcttgttt gccttgcgga tatgtttggt atcgcggtgg gcgggttctg
      421 acgtaccggc agcggcagtt gtcgatacca gtcccaatat tgcagacaac agtatggcaa
      481 gcatcttttt gacagacatg acgatccttt cagtttgtca ttccttccag tataggatgg
      541 aaggatggaa cgggcaaatt gtcggcagtt tcagactcat tcagccgaca aaacttgccg
      601 tttgatatgc agattcaaat aacacattaa tctcaataat aaattccaat cggtaagaaa
      661 atggaatttg tcggcggcgg cgcggcaaaa tcatactttg caaaatttaa caatttgcag
      721 gggcagaaaa caggaagctt tcctttttcg tcggaaaatc cttatttcac cgccttgtag
      781 ccggagccgg tcaaaaggca aaaaatttac ccgtttttta tcggtaaaga attatcagat
      841 aaaacaaata ttataggaaa aatacgacag gcgggtttta tcgcgcattg cctgaaactg
      901 aaaaatacaa ccgttgtcaa gactggagaa aatgccaaaa atccactata ttgtctgcct
      961 taatttattt gaaaagaccg tgtcttgaat atcaagagtg gaagaggaag cgatgaatac
     1021 accgactgat ttgaaagtaa ccaaacgaga cggaagatta gaagccattg atttggataa
     1081 gattcaccgt gtcgtcactt gggcggcgga cggattggaa aatgtttccg tatctcaggt
     1141 cgagttgaaa tcgcacatcc agttctacaa cggcatccgc accgacgaca tccacgaaac
     1201 catcatcaaa gccgctgccg atttaatttc ggaagatacc ccggactacc aataccttgc
     1261 cgcgcgtttg gcgattttcc atctgcgtaa aatagcctac ggcgagtacg agccgccgca
     1321 cctttacgac cacgttaaaa aacttaccga tgccgcaaaa tacgacaggc atatccttga
     1381 ggattacagc cgcgaagaat ttgacgagct gaacgcctat atcgaccacg aacgcgatat
     1441 gtccttttcc tatgccgctg tcaaacagct cgaaggcaaa tatctggtac agaaccgcgt
     1501 tacccgccag atttatgaaa caccgcagtt cttatatgtt ctggtggcga tgtgtctttt
     1561 cagcaaatat cccaaagaaa cacgcctcga ttacgtcaaa cggttttacg atgccgtttc
     1621 cacatttaaa gtatcgctgc cgaccccgat tatgagcggc gtgcgtacgc ctacgcgcca
     1681 gttctcaagc tgtgtgctga ttgaatgcga cgacagcctg gattccatca acgccaccac
     1741 cagcgcgatt gtgaaatacg tttcccagcg tgcgggcatc ggcatcaatg ccggacgtat
     1801 ccgcggtttg gacagcgaaa tccggggcgg cgaagcgcgg cataccggct gcattccgtt
     1861 tttcaaaatg tttcaggcgg cggtcaaatc ctgttcgcaa ggcggcgtgc gcggcggagc
     1921 ggcaactttg ttctacccct tgtggcatat cgaagccgaa agcctgctgg tgttgaaaaa
     1981 caaccgcggt gtggaagaca accgtatccg tcagcttgat tacggcgtgc aaatcaaccg
     2041 cctgctgtac acccgcctga ttaagggcgg caacattacg ctgttttcgc ccaacgaggt
     2101 tccggggttg tacgaagcgt tttttgccga ccaagacgaa tttgagcggc tctatacgaa
     2161 atacgagcaa gacccgaaca tacgcaagcg tatcattccg gctgccgacc tgttttccac
     2221 gctgatgcag gagcgtgccg ggaccgggcg catctacatt caaaacgtcg atcactgcaa
     2281 tacgcacagc ccgttcgatc cgtgcgtcgc gcctgttcat cagtccaact tgtgtatgga
     2341 aatcgccctg ccgaccaaac cgctggacaa tatcaacgat ccgaacggcg aaatcgccct
     2401 gtgtacactg tctgccttta acttgggcgc attaaacagc ttggacgaat tggaagggct
     2461 tgccgacttg accgtgcgtg cgctcgatgc acttttagat tatcagggat atccggtaga
     2521 agccgcgcgt acctctacta tgggtcgccg ttcgctcggc atcggcgtga ttaactatgc
     2581 ttattatctg gcgaaaaacg gtgtccgcta cagcgacggc tccgcgctcg gtctgaccca
     2641 ccgtaccttt gaagccatac agtattacct gctcaaagca tcggcaaacc ttgccaaaga
     2701 atacggtgcg tgcacgctct ttaaccaaac cgtttattcg caaggcaaac tgcccatcga
     2761 cacttacaaa aaagatttgg atgccgtctg cggcgagcct ttgcattacg actgggaaag
     2821 cctgcgtgcc gacatcgtca aatacggcct gcgtaactcc accctgaccg cgctcatgcc
     2881 gtctgaaacc agttcgcaaa tcgccaacgc caccaacggc atcgagccgc cgcgcggatt
     2941 ggtaacggta aaagcatcga aagacggtat cctgaaacaa gtcgtgccgg agtttgaaac
     3001 cctgaaaaat gcctatgaaa ccctgtggca gcttcccggc aacgaaggct acctgaaact
     3061 tgtcggcgtg atgcaaaaat tcgtcgatca ggcgatttcc gccaacaccg cctacgaccc
     3121 gggcaaattt gaaggcggca aagtttctat gaaacaaatg ctcaaagatc tgctgaccgc
     3181 ctacaaatat ggcgtcaaaa ccctgtacta ccacaacacc cgtgacggcg cggacgacac
     3241 gcagaccgat attcaggatg acggctgcgc gggtggggct tgtaagattt gataagggct
     3301 ttggtgaagt aattgaaagc ctttcctaaa tttgattatc aaggtattga atatgatcta
     3361 acgcatttaa ctgcgttttc ttgtgactat attcaacctg ctaaaagtga tggtacggcg
     3421 gaaaaacgat accgctgtat tatcgagttt agtacacatt gttttacaag aggtgaaaat
     3481 aaacgtaagg gagaaacgtt atcagatatt gaacctgcat tgcagtatat aacggctaaa
     3541 gaaacacgaa ttttttgttt cgaacgttat caggtttcaa aaatgttgcc gcaaattatg
     3601 cgtgaaatta gtagaaacaa atgttatttc acttcggctg atgataagtt tctgaccata
     3661 tcagtaacgg ataaaaacgg taaaaaagta gattatgaaa tttacttttc acttcaaaga
     3721 gccaaatctc caaaacatga tgtacatatt tacattaata gtgcatatat acgggatggg
     3781 gattacaaag aaaatcacgg aacaaaagta aggagaaaac cggtcggttt ctttgtgtta
     3841 ttacataata cattggtcaa taagagaatc aaacgcccaa agtgaaacaa ccctcactta
     3901 aaagcaaggg ttgtcagtac aggcttcgct ttataactgc tcttgggcta gtagcctaaa
     3961 aaggtcggac aaacgctgaa atggtttcat gcctgctcat gcgttttgtt gagacaaatt
     4021 gtaccacgat tgcaataagg ctcaaaataa aa
//
LOCUS       DAXHKX010000170         4036 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHKX010000170 DAXHKX010000000
VERSION     DAXHKX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.170"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(92..1342)
                     /gene="glyA"
                     /locus_tag="ACFMWZ_001842"
     CDS             complement(92..1342)
                     /gene="glyA"
                     /locus_tag="ACFMWZ_001842"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC5935750.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1497..1760)
                     /locus_tag="ACFMWZ_001843"
     CDS             complement(1497..1760)
                     /locus_tag="ACFMWZ_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935751.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLPLGSLVLT"
     gene            complement(1823..3598)
                     /gene="ggt"
                     /locus_tag="ACFMWZ_001844"
     CDS             complement(1823..3598)
                     /gene="ggt"
                     /locus_tag="ACFMWZ_001844"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC5935752.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3766..4036)
                     /gene="grxD"
                     /locus_tag="ACFMWZ_001845"
                     /pseudo
     CDS             complement(3766..4036)
                     /gene="grxD"
                     /locus_tag="ACFMWZ_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
BASE COUNT         1009 a          955 c          955 g         1117 t
ORIGIN      
        1 gattttgggg gattttgcaa aggtttccgc ctgaaacatt atgagatttc aggcggcatt
       61 ggattgcttg gcggaatatt tttaaaaagg cttacgcgcc gtaaacgggg tatttattgc
      121 acaaagcagt tacttgttcg cggactttgg cgaggttggc ttcgtcttcg gggttagaca
      181 atacgtcggc aaccaagttc gccaatacgc gcgcgtcggc ttcgttaaaa ccgcgtgtgg
      241 tcatggcggc ggaaccgatg cggatgccgg aggtaacgaa gggtttttcc ggatcgttcg
      301 gaatggcgtt tttgttgacg gtgatgtgcg ctttgcccaa agcggcttcg gcggctttgc
      361 cggtgatttt catcggttgc aggtcgacga ggaaaacgtg gctttcggtg cggccggaaa
      421 cgatgcgcaa accgcgtttg accagttctt ccgccatggc ggcagcattg attttcactt
      481 gttttgcgta ttgtttgaac tcgggttgca acgcttcttt aaacgctacg gctttggcgg
      541 cgatgacgtg catcagcgga ccgccttgca ggcttgggaa gatggaagag ttcaacgctt
      601 tttcgtgggt attgtcgcgg cacaaaatta cgccgccgcg aggaccgcgc agggttttgt
      661 gggtggtggt ggtaacgaag tcgcagaacg gcacgggatt gggatattcg ccgccggcaa
      721 ccagaccggc atagtgcgcc atatcgacaa agaggtatgc gccgacttta tcggcgattt
      781 cgcggaattt tgcccagtcg atttgcaacg cgtaggcaga cgcaccggcc acaatcattt
      841 taggtttgtg ttcgagagcc aagcgttcca cttcggcata atcgagcact tcgttttcat
      901 ccaagccata agtaacggcg ttgtagagtt tgccggaaat attaacgctc gcgccgtggg
      961 tcaggtggcc gccgtgcgct agagacatac ccaaaatggt gtcgcctggt tttaaaacgg
     1021 aagcgtacac ggcttggttg gcttgcgagc cggagtgcgg ttggacgttg gcgtattgcg
     1081 cgccgaacag ttttttgacg cggtcaatcg ccaattgttc gacaatatcg acgtattcgc
     1141 agccgccgta gtagcgtttg ccggggtagc cttcggcgta tttgttggtc agctgggaac
     1201 cttgcgcttc cattacggcg cagctgacgt agttttcaga ggcaatcagc tcgacgtggt
     1261 cttgctggcg ttggtcttct tgggcaatgg ctgctgccaa atcggggtca tattgtgcga
     1321 gggtaacgct ttttgaaaac atgttctcgg ctcctttgtg taatcagggt atcatgagtg
     1381 ttttttgtat aaaaaaatac ttcaaaacct aaggcagata gtccataatg cgtaaatttt
     1441 ctttggcatt atcaggtaat ttatttaaca tgctggtttt tagcgtctca ttacctttat
     1501 gttagtacaa ggctacccaa aggcaataca tcaaatggta aattttcggc agtttgtgca
     1561 aaatcaatca aataaccagc accaaccaca tctttatcag ccataacccg ctgatacacc
     1621 acttgccaag acaactctcc tgccccacca tgtcttaaaa tcagatgaat ggcttgtaat
     1681 gctgtgtttg gcgtctcaca aattgcgaag atctgctcag aagcttgctt gatggcttgt
     1741 atttttgttt gattactcac aatttccccc tattttaata ttaacttaat gcggtcaaat
     1801 tcacaaaata caagcttcac ctctaatcac ccatcactcg accttctcgg cgtggatcgg
     1861 caccaccaac cagcctgctt ggctcgataa taatggcttg aacacctgaa tttagctcac
     1921 gcacgtcagt cttatagccc aaatcattta atgcttgttg ccactggagg gcggttgtac
     1981 ccgtttctag ttcatagcta ccaaagcgat ttaataaatt gggtgcactg atggcatctt
     2041 ggatatccat attccagtca ctatgtgcca caatcgtctt agcgacatag ccaatgatac
     2101 ggctaccacc tggggagccg attgccatat aaggcttgcc tgctttaaat acgatggttg
     2161 gtgccattga ggagcgtggt ctcttgccgg gctcgacacg attggcgacc tgtttgccct
     2221 gctttattgg ctcaaaacta aagtctgtca gctcattatt cagcaggtag ccatttgcca
     2281 tcaaagttga gccaaacgca ttttcaatgg aagtcgtcat tgatagcaca ttgcccgcct
     2341 tatccacaat tgatatatga ctggtagaag gtaactcaat cgcttgtgag gacacccact
     2401 catgaataaa atcgcctgca gatacgctag gcaatgcctt atccgactgc tcaagcagct
     2461 ggctgcgatg ttttaggtag tctttagaaa tcaactggcg aatgggtact ggtacaaaat
     2521 cagggtcgcc caaatataca tcacgatccg caaacgcaag cctagaagcg tcgcccaaga
     2581 gacgtaaacc ttcagcatca taccccacct gattgggtga aaattcattt aaaatcccca
     2641 aaatttgacc cacagcaatc ccacctgagc ttggtgcacc cataccgcat acttcataaa
     2701 tacgataagt cacacaaaca ggcgggcgtt ccaccacttg ataatcagat aaatcttgta
     2761 aggataattg accggggtta tccttagcat tttggacaac tgaaacgata ttttgggcat
     2821 atttaccagt atgcagagct tttgcacctt gagctgctaa cgcctgaaca ctgtcagcaa
     2881 attctaaatt tttcagcaag ctgcctgctt gtagcggcac accattcggc aaaaaataag
     2941 cggctgtttt tggatagcgt gccaaatgct gctgattttg ctcaaccgag atggcaagcc
     3001 ttggcgacac ctcaaagcct tgttttgcca agcggatcgg tgtatcaaat aattttcccc
     3061 aaggcaatac accgtatcgc tgatgtattg tctccatcag tttagggata gcaggcgtac
     3121 ccaccgagcg accaccgacc accgcttcca taaatttcaa tggttgacca tctttatcca
     3181 aaaataattc cggcgtcgca cgcatcggtg ccgtctcacg cccatcaaat gtggtcaatg
     3241 ttttggcggt attatcccaa tacaacacaa atgcaccacc gcccaagcct gacgactgtg
     3301 gctctaccaa gcttagtgtc gtctgcaccg ccaccatcgc atctgcagcg ctaccgcctt
     3361 gctttaagat atcatagcca gcttgtgttg ctaatggatt ggctgacgct accataaaat
     3421 cacttgcaat cacctgcttt tgttcgatca gtcccgttgc atgttcaggc gtgtgagcgt
     3481 ctgcacctgt gatgacagca gaatgagtat taaccttacc ttgattggca tggatgactt
     3541 gacatccaga gattgtcata gacattatca atgcagtcaa taaatatgtt ttagccacaa
     3601 gcactccttc gcctgagttt gattgatgat tcatacaagg catgctgatt attgtattta
     3661 atatggctaa ataattcaat ccaaactatc aatcttgacc atcaaaaaaa gaccgctaat
     3721 gtcatcagca gtcttttttg atatttattt taagatatta agtaatcaga cctttgggct
     3781 atgctcttca atgagtggtt ttagctcacc tgattggtac atttgtagga taatatcact
     3841 accaccgatt aactcaccat taacccaaag ctgtggaaag gttggccgat tggcgatgag
     3901 tggtagagta ctgcgaattt ctgggttttc taggatatta acaaaagcaa agggtctgcc
     3961 aattgggtca gcacctctac tgcacgcgct gaaaatccac attggggaaa ctggggcgtg
     4021 cctttcatat atagtg
//
LOCUS       DAXHKX010000171         4005 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHKX010000171 DAXHKX010000000
VERSION     DAXHKX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.171"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(43..768)
                     /gene="aat"
                     /locus_tag="ACFMWZ_001846"
     CDS             complement(43..768)
                     /gene="aat"
                     /locus_tag="ACFMWZ_001846"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC5935753.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVVCKGL"
     gene            complement(838..1272)
                     /gene="fur"
                     /locus_tag="ACFMWZ_001847"
     CDS             complement(838..1272)
                     /gene="fur"
                     /locus_tag="ACFMWZ_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC5935754.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1491..1868
                     /locus_tag="ACFMWZ_001848"
     CDS             1491..1868
                     /locus_tag="ACFMWZ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC5935755.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            1878..2687
                     /gene="dapB"
                     /locus_tag="ACFMWZ_001849"
     CDS             1878..2687
                     /gene="dapB"
                     /locus_tag="ACFMWZ_001849"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC5935756.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2684..2929)
                     /locus_tag="ACFMWZ_001850"
     CDS             complement(2684..2929)
                     /locus_tag="ACFMWZ_001850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935757.1"
                     /translation="MPRRQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(2947..3357)
                     /locus_tag="ACFMWZ_001851"
     CDS             complement(2947..3357)
                     /locus_tag="ACFMWZ_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935758.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLFNYTLKNNEFHIIVNCVDYPDLP
                     QEIPMPNYSDWVKIKFKQFSYLKFVYGYATKNRDKDIDNTLELGELKKDDEILDYGGA
                     LEVIGGRYDFPTAFRIDIVCREIELEFLDQEGFN"
     gene            3578..3967
                     /locus_tag="ACFMWZ_001852"
     CDS             3578..3967
                     /locus_tag="ACFMWZ_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC5935759.1"
                     /translation="MKGLDKYVLFKDCETLGELFRIYRDYLSLNIDDPNLKIRFFDFL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRRIWPDMEEMLLYFPDNPWFYVEHF
                     WWGATCPIELTQLPKIEIYEEQMKQGK"
BASE COUNT         1104 a         1137 c          842 g          922 t
ORIGIN      
        1 ataatccacc atatttgaac ctgaccgaag cataagccgc catcaaagcc ccttgcacac
       61 cacttcgcgc cgcccgattt cctctttcaa cggcacggcg ttcaacatcc gcagacgttc
      121 ggcaaaatcc gcaaacggca gcagctccga accgaaacgg cgcatatgtt ccgtgtcctg
      181 ctggcagtct atcagttcca cacccaaatc cgccaaaaac ggcacggcgc aggcaaacgc
      241 gattttcgac gcatccggtt gtaatgcgaa catcgattcg ccgtaaaaca ccctgccgat
      301 ttgcacgccg taaaagccgc ccgccaacct cgtttcaccg ctttcatcgg gataatggca
      361 ctcgaacgaa tgcgcgtgtc ccatttcgtg cagcttcaaa tatgccgtct gaaactcggg
      421 cgcaatccaa gttccgtcct gattcgggcg cgccgctgcc gcacaatgcg cgaccacttc
      481 cgcaaaacag ccgttgaccg caacccgata gctgccgttg cgcagcgttt tcgccagcga
      541 gcgcggaacg tgcagacgtt cgggaacaat caccgcacgc gggccgaccg cataccaaaa
      601 aaaccaaccg tcccgagaaa accacggaaa cacgccgttc cgatacgcct cgagcagccg
      661 ccccgcatcc aaatcgccgc tcacgccgac cagcccgtcg caccgggcca aagcataggt
      721 aggatcggga aaggcataat tgtcaggggc aagcagcgga atacgcatgg cagttctcca
      781 aaaacacaga cggcatccaa aatcatgaat gccgtctgaa caacaaaccg tccggattta
      841 acgtttgccc ttggcctgac agtcgctgca cacgccgtac atataaagcg cgtgatcgac
      901 gatgcggtag ccgttttcct cagcgatttt gtcttgcagg gcttcgattt cgggattgtg
      961 gaattccgtt acctcgccgc acttcacgca gacgatgtgg tcatggtggt cgcctttgtc
     1021 caactcataa accgccttgc ccgtttcaaa atgatggcgt tgcagaatac ccgcctgctc
     1081 aaactgggtc agcacacggt aaatcgtcgc cacaccgatt tccacaccct cttccaacaa
     1141 aatgcggtac acatcttccg cactcaaatg ctcttccgaa tgcgtctcga acaaatccaa
     1201 aatcttcaaa cgcgggccgg taaccttcag accgctgtct ttcagttgtg caatattgtt
     1261 gaatttttcc ataatattca atacccctgt aaaacaatag acgttataat acgcaatttc
     1321 ggcctgtttg cccactatcg caccatagca gtttgcaata gtaaaaccgc accggcggcc
     1381 gatgcgcgga cagggcggat accgcttaat cgtatgatta tcgttcgctt ttataaaata
     1441 ttcaagcagt gttacactac ccatcccgat ttgcacagaa aggcatttcc gtgaacaaaa
     1501 ccctcatcct cgccctttcc gccctccttg gccttgccgc gtgcagcgtc gaacgcgttt
     1561 cactgttccc ctcgtacaaa ctcaaaatca tacagggcaa cgaactcgaa cctcgcgccg
     1621 tcgcctccct ccgccccggt atgaccaaag accaagtcct gctcctgctc ggcagcccca
     1681 tactgcgcga cgcattccat accgaccgct gggactatac cttcaacacc tcccgcaacg
     1741 gcatcatcaa agaacgcagc aatctgaccg tctattttga aaacggcgtg ctcgtccgca
     1801 ccgaaggcaa cgccctgcaa aatgccgccg aagccctccg cgtaaaacaa aacgcagaca
     1861 aacaataagg aacacacatg acaccgctca aaatcgccat cgccggcgca aacggccgta
     1921 tggggcgcgt cctcgtcgaa gccgtcaaca accatcccga caccgtcctt tccggtgcgc
     1981 ttgaacactc aggctcagaa gccctcgggc tggacgcagg ctacgccgtc ggactcaaaa
     2041 ccggcatcgc catttcagac gacgttgacg ccgttctcgc acaaagcgac gtactcatcg
     2101 acttcacccg ccccgagccc accctcaaac acctgcaaaa atgcgttgaa aaacaagtca
     2161 acatcatcat cggcacaaca ggcttcgacg atgcgggcaa agccgctatc cacactgccg
     2221 ccgaacaaac aggcatcgtt ttcgccgcca acttcagcgt cggcgtcaac ctcaccttcc
     2281 acatcctcga caccgtcgcc cgcgtgttaa acgaaggcta cgacatcgaa atcattgaag
     2341 gccaccaccg ccacaaagtc gatgccccca gcggcaccgc gttacgcatg ggcgaagtca
     2401 tcgccggcgc gctcggcaga gacctcaaac aatgcgccgt ttacggccgc gaaggccaca
     2461 ccggtccgcg cgatccgtcg accatcggct ttgccaccgt ccgcgcaggc gacatcgtcg
     2521 gcgaccacac cgccctcttc gccaccgacg gcgagcgcgt ggaaatcacc cacaaagccg
     2581 gcagccgcat gacctttgcc gccggcgccg tccgcgccgc agtttgggta aacggcaaaa
     2641 cgggtttgta cgatatgcag gacgtactcg ggctgaacaa ccgttaaccc ccatacaaaa
     2701 tgccgtctga aaagatattg ttcagacggc attttgccga caggctccgt atcggcatat
     2761 gtttcaacac acgggacgat acataaagcg tcgccctatg tgtcgtcctg atttggaagg
     2821 gattacgccc ctcccaaata aagtctgatt ctaccgcccc gaaggacaga tgtccgagtg
     2881 gcggggtttc aaccgaaaag gaaatacgat aaagttgcct gcgcggcata acaagctacg
     2941 gctcgtttaa ttgaaaccct cctgatctaa aaattctaac tctatttccc ggcaaactat
     3001 atctatccta aaagcagtcg gaaaatcata cctaccgcct atcacttcca gcgcacctcc
     3061 ataatccaag atttcatcat ccttctttaa ttcgccaagt tccaatgtat tatcgatatc
     3121 tttatctcgg ttctttgtgg cgtatccata gacaaatttc aaatagctga actgcttgaa
     3181 tttaattttt acccagtctg aataattagg catagggatt tcttgaggta aatccggata
     3241 atccacacaa ttgacgatga tatgaaattc attgtttttc aaagtatagt tgaatagagt
     3301 gccctcatat atatacagaa attgataatc tttttttgga atagtataag ttgtcatatt
     3361 attttacctt ttcaaaaaca atggttttaa gattttttga gtccaagttg gttccactac
     3421 tcttatgaaa tttcagttgt ttaaactctt ccttcatttt aaggaatttt tcaactagcc
     3481 gagaacaaac tggagcaaaa tgggcgattg agtttcaaca tcatccatta ataggtagga
     3541 aaaaaatgga attaaaattt agataaggaa ttgaataatg aaaggtttag ataagtatgt
     3601 cctatttaaa gattgtgaaa cattaggaga attgtttcga atatatcgag actatctatc
     3661 attaaatatt gatgacccaa atttaaaaat tagatttttt gattttttgg aagaactttt
     3721 atcagatggt gttattgaat tatgcgatta tcgtgaaaat cctccaaaaa tattaactgg
     3781 ttcgccaaaa acacaggtag atgaattaag aagaatttgg ccggatatgg aagagatgtt
     3841 gctatatttt ccagataatc cttggtttta tgtagaacat ttttggtggg gagcaacttg
     3901 ccctattgaa ctgacacaat tgcctaaaat agaaatttac gaagaacaaa tgaaacaagg
     3961 taagtaagcg tattagtata agctataatc ataaatcata tataa
//
LOCUS       DAXHKX010000172         3988 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHKX010000172 DAXHKX010000000
VERSION     DAXHKX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.172"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="ACFMWZ_001853"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="ACFMWZ_001853"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC5935760.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="ACFMWZ_001854"
     CDS             1160..2290
                     /locus_tag="ACFMWZ_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC5935761.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2361..3212
                     /gene="lgt"
                     /locus_tag="ACFMWZ_001855"
     CDS             2361..3212
                     /gene="lgt"
                     /locus_tag="ACFMWZ_001855"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC5935762.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="ACFMWZ_001856"
     CDS             complement(3295..3957)
                     /locus_tag="ACFMWZ_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC5935763.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEEFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          866 a         1179 c          984 g          959 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcgg aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttca gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccgcgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatgatcgtc
      601 gcgtccgctt acgccgaccg cgtgcccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacgtgc ccgcccaaca ccatttcgac aatatccatc gtctctttgt cggtcacgcg
      721 cattccttgg acaaactcgc cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgataa cgggatggat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtact tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgctttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacgactccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatctggca gaccttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgacc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catacgccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aaggccgcga catcaatctg ctgaccatcg gcaatcaggt
     1621 cgaaagcgat ctgaaaatct ggattaccgc ccgccagcat ccgggcgaaa ccatggcgga
     1681 atggtttgtc gaggggctgc tcggcaggct gctcgaccct caggatgcaa ccgcgcgcac
     1741 cctgctcgat cgcgccacgt tctatatcgt ccccaacatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tgtggaaaaa agcccggaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggcgtgga
     1921 tttgttcttg gacatccacg gcgacgaagg gctgcccttt atctttgttg caggaactga
     1981 aggcgtgcca aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacatg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccgttcaaag acaatgccaa cctgcccgac gacgatttcg gatggaacgg
     2221 tcaacggtcg ttgcgcttgg gcgaagccgc gttgtccgcc atcctcaaca tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcccgtt atgattaccc atccccaatt cgaccccgtc cttatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg
     2521 acgacttcct gacatggggc attttgggcg taattttggg cgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctt gttccgctgg
     2761 gtctcgcttc aggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gtatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 ggttttttcc ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg
     3421 gtaaatgtcc gccagcgcgt aaggcaactc ggattccagc tccgccagct cgccttctgt
     3481 gaattgcagc cggatagcgc cgttttcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa attcttcatc atcggcggct tcaaaccaat cggtcggcac
     3601 aatatccaag ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccct
//
LOCUS       DAXHKX010000173         3916 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHKX010000173 DAXHKX010000000
VERSION     DAXHKX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.173"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..319
                     /locus_tag="ACFMWZ_001857"
     CDS             <1..319
                     /locus_tag="ACFMWZ_001857"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC5935764.1"
                     /translation="QQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLINTSSLPII
                     SMKELKKLKIPIPSLPEQEKIVAILDKFDTLTHSVSEGLPREIALRRKQYEYYREQLL
                     AFR"
     gene            306..527
                     /locus_tag="ACFMWZ_001858"
     CDS             306..527
                     /locus_tag="ACFMWZ_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935765.1"
                     /translation="MPFGRVGFSPPKQRQAATTVKQRQAGRWAEAHPTEDGNRPVFRR
                     SVRKPENMAIDAQNKEKNPIGMIQTALVG"
     gene            752..3850
                     /locus_tag="ACFMWZ_001859"
     CDS             752..3850
                     /locus_tag="ACFMWZ_001859"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC5935766.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLLVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLN
                     PLKTATIFSFAANEEQNAVGEIVDETFEPEAMDGSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKHTKNVVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELRERFPEPDEIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLISSLKRGYASENGTELTETLPKMSPINPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
BASE COUNT         1145 a          970 c         1006 g          795 t
ORIGIN      
        1 ccaacaactg accaatttta caattaaaga tgaatttaag gatgctttag atataaattt
       61 cgctttctat tattttttca ttattgcgga gcagagcaaa aaacttatta atacttcaag
      121 tttacctatt attagcatga aagaattaaa gaaattaaaa atccccatcc cctcactccc
      181 cgaacaggaa aaaatcgtcg ccatcctaga taaattcgat accctgaccc actccgtcag
      241 cgaaggcctg ccgcgcgaaa ttgccctgcg ccgcaagcaa tacgaatatt accgcgaaca
      301 gctgcttgcc tttcggtagg gtgggcttca gcccaccgaa gcaacggcaa gctgcaacaa
      361 cagtaaaaca acggcaggcg gggcggtggg ctgaagccca ccctacggag gacggcaacc
      421 gccccgtatt ccgccgcagc gtgcggaaac cggaaaatat ggcgatagat gctcaaaaca
      481 aagagaaaaa cccgattggg atgattcaga cggcattggt ggggtagaag tcgcagacaa
      541 agtagggtgg gcttcagccc accgaaacaa cggcaagttg caacaacagt aaaacaacgg
      601 caggcgaggc ggtgggctga agcccaccct acgatggacg gcaaccgacc cgcattccac
      661 cgaagccaac ctaaagcagc ctgcacattt tcaggcagcc caatccgaac atcaaaccgt
      721 ttaaaacccg accctgcaaa ggacgaccca tatgaacctt gaaaccaaac ccatcgccga
      781 aacgccgaat ttcatcgtgc tcgaccaata tgaaaaaatc gaacagtcgg gcagctacca
      841 atcggaaaac cggttggaag cggagttaat cgccgatttg cagaatcagg gttacgaata
      901 ccgcaaggat ttgaacagcc aaagcaggct gctggaaaac ctgcgcgcgc agttgcagcg
      961 gctgaacgat gtggcgtttt cagacggcga atgggcgcgg tttttgacgg aatatctgga
     1021 caggccgtct gaaaatatta ccgataaaac ccgcaaaatc cacgacgacc atatttacga
     1081 tttcgctttt gatgacggcc gtctgaaaaa catttatctg ctggacaaga aaaaccttgc
     1141 ccgcaaccat gtgcagctta tcaaccagtt tgagcagacg ggcacgcatg caaaccgcta
     1201 tgacgttacc gtgttggtaa acggtctgcc gctggtgcag attgaattga aaaagcgcgg
     1261 tgtggcggtg cgtgaagcat tcaatcaggt gcaccgttac agcaaagaga gcttcaacag
     1321 cgaaaattcg ctgttcaaat tcctgcaaat cttcgtgatt tccaacggca cggacacgcg
     1381 ctatttcgcc aacaccacca agcgcgacaa aaacagcttc gatttcacga tgaattgggc
     1441 gcggtcggac aatcatccga ttaaggattt gaaagacttt accgccacgt tcctgcagaa
     1501 aagcgtattg ctgggcgttt tgctgcatta cagcgtgttc gatgcgaatg atacgctgct
     1561 gattatgcgg ccgtaccaga ttgccgccgc cgaacgtatt ttgtggaaaa tcaacagttc
     1621 ggcgcaggcg aagaattgga gcgggccgga aagcggcggc tatgtttggc ataccacggg
     1681 cagcggcaaa acgctgacca gctttaaggc ggcgcgtctg gcgacggaat cggcgtttat
     1741 cgacaaggtt ttcttcgtgg tggacaggaa ggatttggac tatcagacga tgaaggaata
     1801 ccaacgtttt tcgcccgaca gcgtgaatgg ttcggaaagc acggcgggct tgaaacgcaa
     1861 tttggaaaaa gacgacaaca aaatcatcgt taccaccatc caaaagctga acaacctgat
     1921 gaagagtgaa gataatctgc tggtttacca tcagcaggtt gtatttattt tcgacgaatg
     1981 ccaccgctcg caattcggcg aagcgcaaaa aaacctgaaa aagaaattta agaaattctg
     2041 ccagttcggt tttaccggta cgccgatttt tcccgaaaac gctttgggcg cggaaaccac
     2101 ggcgggcgtg ttcgggcggg agctgcattc ttatgtgatt accgatgcca tccgcgatga
     2161 aaaagtattg aaattcaaag tggattacaa cgacgtgcgc ccgcagttca aagccgtgga
     2221 agcggaacag gacgagaaga aactgagtgc cgccgaaaac cgccaagccc tgctgcaccc
     2281 cgaacgcatc cgcgaaatca cgcaatatat cctgaatcag ttcaggcaga aaacgcaccg
     2341 cctgaacgcg ggcggcaaag gctttaacgc catgtttgcc gtcagcagcg tggatgcggc
     2401 gaagtgctat tacgaagcgt tcaaaacgca acaggcaggc agcctgaacc cgttgaaaac
     2461 agccactatt ttttcctttg ctgccaacga agagcagaac gccgtcggtg aaattgtcga
     2521 tgagactttt gaaccggaag cgatggacgg cagcgcgaaa gaatttttgc aggctgccat
     2581 caacgattac aacgcctgtt ttaaaaccaa tttcggcacg gacagcaaag cctttcaaaa
     2641 ctactaccgc gatttggcaa aacgggtgaa aaatcaggaa gtggatttgc tgattgtggt
     2701 cggcatgttt ttgacgggtt ttgacgcgcc gacgctgaac acgctgttcg tcgataaaaa
     2761 cctgcgctat cacggcctga tgcaggcgtt ttcgcgcacc aaccgcattt acgatgccac
     2821 caaaaccttc ggcaatattg tctgcttccg cgatttggag caggcaacca ttgatgcgat
     2881 taccctgttc ggcgacaaac acaccaaaaa cgtggtgctg gaaaaaagct acgaagaata
     2941 catgaacggc tataccgaca gccagaccgg cgaagcgcgg cgcggttatt tggatgtggc
     3001 aaaagagttg cgtgaacgtt tccccgaacc cgacgaaatc gaaacggaaa aagacaaaaa
     3061 agattttgcc aaactcttcg gcgaatacct gcgggcggaa aacgtattgc agaactacga
     3121 tgaatttgcc gcgctgcgcg agttgcagag tgtggacgcg gcggacgaag atgcgatgaa
     3181 ggcgtttcaa gaaaaatact acttgagcga tgaagacgtg caggaaatgc ggcaagtgcc
     3241 gatgccgtct gaaagggcgg tgcaggacta ccgttccgcc tacaacgaca tccgcgattg
     3301 gttgcgccgc caaaaagcag gcgaacagaa agaacagtca aaaatcgatt gggacgatgt
     3361 ggtttttgag gtggatttgc tcaaatcaca ggaaatcaat ctggattaca tcctgcaact
     3421 ggttttcgaa caccacaaaa aaatcaaagg caaagcggag ctggtggaag aaatccgccg
     3481 catcatccgc gccagcatcg gccaccgcgc caaagagggt ctgattgtgg atttcatcaa
     3541 cgatacggat ttggacaaag tacccgacgt tcccgccata ctggaaacct tctacaccta
     3601 cgcgcaagag gtgatgcggc acgaagcggc aggattgatt gccgccgaag gcctgaacga
     3661 aaccgccgcc aaacgctatt taatcagctc tctcaaacgc ggctatgcca gcgaaaacgg
     3721 cacggaactg accgaaaccc tgccgaaaat gagtccgatc aacccgcaat atctgacgaa
     3781 gaaacaaagt gtttttcaaa agattgcggc gtttgtggag aagtttgccg gaatagggac
     3841 cgatatttga caaaatgccg tctgaacctt tcagacggca tttttgattt catgcgaggg
     3901 cggtttttat agtgga
//
LOCUS       DAXHKX010000174         3900 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHKX010000174 DAXHKX010000000
VERSION     DAXHKX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.174"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            180..1697
                     /locus_tag="ACFMWZ_001860"
     CDS             180..1697
                     /locus_tag="ACFMWZ_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC5935767.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCQQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLSPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFLTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1758..3539)
                     /locus_tag="ACFMWZ_001861"
     CDS             complement(1758..3539)
                     /locus_tag="ACFMWZ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC5935768.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPNFGIFETL
                     RAENGRCTLLDRHLCRLKTSARALNLPLPDGCENQIKQYIADLPDGAFRVKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTHRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKYQGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          817 a          868 c         1276 g          939 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactataa atgtgaaatc cgccctttga aaatcgggcg
       61 gcggtttttt gcctgcccgc tttcagacgg caaagccggt tttcacgggt ttctgcctgt
      121 ttttcggatg gtttgacgtg cttcggcggc gtgtttgcca gaaaggtaaa tgacagggta
      181 tgttgtattt cagatacggt tttttggttg tttggtgtgc ggcaggtgtt tctgccgcct
      241 atggggcgga tgcgcccgcg attttggatg acaaggcatt gttgcaggtg cagcggtcgg
      301 tgtcggataa gtgggcggaa tcggattgga aagttgaaaa tgatgccccg cgcgtggttg
      361 acggggattt tttgttggcg catccgaaaa tgttggaaca tagtttgcgc gacgcgctca
      421 acggcaatca ggcggattta atcgcttcgt tggcggattt gtatgccaag ctgccggatt
      481 atgatgcagt tttgtacggc agggcgcggg ctttgctggc gaaattggcg ggaaggccgg
      541 cggaggcggt ggcgcggtat cgggaactgc acggggaaaa tgcggcagac gagcggattt
      601 tgctggattt ggcggcggcg gagtttgacg atttccggct gaagtcggca gaaaggcatt
      661 ttgcggaggc ggcaaaatta gatttgccgg cgccggtttt ggaaaatgtg gggcgtttcc
      721 ggaaaaaaac ggaggggctg acgggctggc gtttttcggg cggcatcagc ccggcggtca
      781 atagaaatgc caataatgcc gcgccgcagt attgccagca aaacggaggc cggcagatat
      841 gcagtgtcag ccgggcggag cgggcggcag ggttgaatta tgaaatcgag gcggaaaagc
      901 tgacggcgtt ggcggataat cattatttgt tgttccgttc caatatcggc ggcacgagct
      961 attatttcag taaaaaatca gcttatgatg acgggttcgg cagggcgtat ttgggttggc
     1021 agtataaaaa tgcgcggcag acggcgggga ttttgccgtt ttatcaggtg cagttgtcgg
     1081 gcagcgacgg ctttgatgcg aaaacaaaac gggtaaacaa ccgccgcctg tcgccgtata
     1141 tgctggcgca cggagtcggc gtgcagctgt cccatactta ccgcccaaac ccgggatggc
     1201 aattttcggt cgcgctggaa cattaccgcc aacgctaccg cgaacaggat agggcggaat
     1261 acaataacgg caggcaggat gggttttatg tttcgtcggc aaaacgtttg ggcgaatcgg
     1321 caactgtgtt cggcggctgg cagtttgtgc ggtttgtgcc gaaacgcgaa acggtgggcg
     1381 gcgcggtcaa taatgccgcc taccggcgca acggtgttta tgccggctgg gcacaggagt
     1441 ggcggcagtt gggcggtttg aacagtcggg tctccgcgtc ttatgcccgc cgcaactata
     1501 agggcgttgc ggctttcttg acagaggcgc aacgcaaccg cgaatggaat gtctcgctgg
     1561 ctttgagcca cgacaagttg tcgtacaaag gtatcgtgcc tgcgttgaat tatcgtttcg
     1621 gcaggacgga aagtaatgtg ccgtatgcga aacgccgcaa cagcgaggtg tttgtgtcgg
     1681 cggattggcg gttttgaatg gtgggataat gccgtctgaa ccttgtggaa acaggttcgg
     1741 acggcatttt tgcgcgttca ggcaagggcg gcggcaaata cgccgcgcaa ggcgttggag
     1801 aggcggattt cttcggcttc ttgcagtgtt ttttgtgtga tgtgtgtttc gattacttga
     1861 tttgtttgca aatatttttg cggttcgtcc aatacggctt ggcgcattat gccgtttaaa
     1921 atgtctaaat ctaaagacgg tgtgagccat tgcccttggt atttgacgaa cacgttgctt
     1981 ctgccgcctt cgagcaggat gccgtctgaa ttgaaaaaca ggctgtcgaa cgcgccttgt
     2041 gtttcggcgg tttgccacgc ttggtcgaag agggcgcggt gtgtggtttt gaagcggcgc
     2101 aggtagtttt gtgcgggcag gatggtcggc gaaatgatga cgcgttgttt gtcggtcaga
     2161 cggtttaaaa cggcgcggga caggctgatg ccgtctgaag cgagcagggc tttgacgcgg
     2221 aacgcgccat cgggcaagtc ggcaatgtat tgtttgattt gattttcgca gccgtcgggc
     2281 aggggcaggt tgagggcgcg tgcggaggtt ttcagacggc ataggtggcg gtcgagcagg
     2341 gtgcagcgtc cgttttccgc gcgcagggtt tcaaaaatgc cgaagttggg gcgcaattcg
     2401 ttgaggaaac gggctttcca gccgcattcc cgatattcgg cggcggggtc gctgtcgatg
     2461 acgatgccgg aaccgacacc gtacacgcct tgataaatgc cgtctgaaag cggcgtgagc
     2521 gacaaggtgc ggataacgac gttgaacgtg ccttcaaacc ccaagccgcc ggaacacggg
     2581 ttcaaatagc cgatgctgcc cgtataaagt ccgcgcgctt cggcttcgag cgattcgata
     2641 atctgcatac tcattttttt gggcgcgccg gtgatgctgc cgcaggggaa ggcggcgcgg
     2701 aggatgtcgg cgaacgaggt gtgcggcaag gcttgggctt ggatggtgct ggtcatctgc
     2761 caaacgctgc cgaaacgcga tactttaaac ggttcgggta cgcatactgt gccggtttgg
     2821 gcgattttgc cgagatcgtt acgcagcaaa tcgacaatca tcacgttttc ggcgcggttt
     2881 ttcgggtctg cttgcaactc ggcggcgcgg cgttcgtctt gtccgtcgcc caaaatcggc
     2941 gcggtgcctt tcatcggttc ggtgctgatg gtgccgtccg aaccgatttt gaggaagagt
     3001 tcgggcgaga aacacagcgt ccacgcggat tgcccttgtg catcgggcag gtgggacaaa
     3061 acggcatagg ggacgggctg gcgcaggcgt tggtaaagtt tgacgggatt gccgtaggct
     3121 tgcaggtgca ggcgggtagt gtagttgatt tgataggtgt cgccgcgtcg gatggcttcg
     3181 tggatttggc ggatatggtc gaggtaatcg gcttcggata cggaggattg cggcgtggaa
     3241 atgccggcgg gaaggtcgtc tgagtgtcgg gcaagccagc ttgcggcatc ggtgtcggcg
     3301 cagtcggcaa accagtgcag ggcaaggttg ccgccgcgct cggactcaac ccccgtcagc
     3361 ggcaaaccga atccgtagtc tgcaaacaac acggcatgca gccctttttg ccagcccgat
     3421 tgcagcgcgc cgtccaaagc atcgagttct tcgggacgga aaaaacggct ttccacatga
     3481 ttttgatagc gttttgcgcg gccgcttacg gcatcgtcaa acagggcgaa ataaggcatg
     3541 gcaattcggg gcaaatgttt tgattatacg ctctttttac acatgttttc agacgattcg
     3601 gcaaatatcg gcaaaatgta attttatgta gagaaagtgg ggggcgaagg tgtaaaattg
     3661 tgaaaacaaa ttccgatttc caacctgaaa tacaataagg agacctttat gacagaccac
     3721 cagctgcaac cgtttgaaaa cgtagaatta ggcgaaaagc aagaccagct ccaagtatag
     3781 tggattaaca aaaaccagta cagcgttgcc tcgctttagc tcaaagagaa cgattctctc
     3841 aggtgctgaa gcaccaagtg aatcggttcc gtactatctg tactgtcttc ggcttcgttg
//
LOCUS       DAXHKX010000175         3886 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHKX010000175 DAXHKX010000000
VERSION     DAXHKX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.175"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..2118
                     /locus_tag="ACFMWZ_001862"
     CDS             <1..2118
                     /locus_tag="ACFMWZ_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC5935769.1"
                     /translation="GKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFGG
                     ESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMNPE
                     GAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKAFGVRANGKLRH
                     GDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLFG
                     APDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVCL
                     NATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLTARGEFETSPVTHN
                     WSTAFDRVIRQRKTIRGTAAGAGKVEVKANENIANQLASFKADYPNSWENSANLDANI
                     KVNSLALSDTLGFVDNKYRLTLGGRFQTVEYTDKKSQSGDAKRFSPMLMAAWVPQPDL
                     VVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKRPG
                     YWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANLLN
                     NTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNGN
                     VSYFGKSYQDTQKQYAFPSYTLIDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQRG
                     QYDRSFAVVGMPRTYWLKAELDF"
     gene            2242..3792
                     /locus_tag="ACFMWZ_001863"
     CDS             2242..3792
                     /locus_tag="ACFMWZ_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC5935770.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         1092 a          970 c          981 g          843 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gagaaagcaa cacgctgacc ggactgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttggca ggtatgtacg gcacgcaagg cacggccagc
      241 gtgcaagtcg gctccgcaca actgattaaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgaaggtg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgacttg
      421 ggtcaacgct tcggcgaaaa caaagcattc ggcgtacgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtt tggcgcgccc
      661 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      721 atgcttacgt ttgaatggga tgcgtttgat aatgcacaga ttacaggcgg tatcggctac
      781 aacaaggcac gctattacgg cacgctgatt tccccgaccg tatgtttgaa tgctaccagc
      841 acttgtaccg atatgcaaaa cacaaacgcc agcggcaaag taacggcacg caactatgac
      901 tacaataccg gtactgcgcg cctgaccgac caatatttcc gcacattgag tatgaacctg
      961 accgcacgcg gcgaatttga aaccagcccg gtaacacata actggagtac ggctttcgac
     1021 cgcgttatcc gccagcggaa aaccattcgc ggaacggcag ccggcgctgg caaggtagaa
     1081 gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt tcaaagccga ctaccccaat
     1141 tcttgggaga actctgccaa cttggatgcc aatatcaaag tcaacagcct ggctttgtcc
     1201 gacaccttgg gcttcgtgga taacaaatac cgcctgactt tgggcggacg tttccaaaca
     1261 gtcgaatata ccgacaaaaa atcacagagc ggcgatgcca aacgcttcag cccgatgctg
     1321 atggcggcgt gggtgccgca acctgatttg gttgtgtacg gcaactatat ggaagacttg
     1381 gagcctgccg atatcaaaac cgatgattcc ggcgaaacga caatggcgaa accgcgtgtc
     1441 agccgccagt ttgaagtggg cgtgcgtaaa aactggggca atttcgtaac cacgttaaac
     1501 gcgttccaaa tcaaacgccc gggctactgg cgcggtcata caactaccaa gacaacaaaa
     1561 ggggtaacaa caacgctgac ctatggcaac aattccgatt tcgcccgcta taaggcgcaa
     1621 ggcggcgcgg ccggcgacga gcaaggtatg gaacgcaatc gcggtattga gttcaacacc
     1681 tacgcgaact tgctcaacaa tactttgcgt ccgactttgg gtctgatgta tctgcaatcg
     1741 accgtgaaag agtatccaaa ttcacgcgat atgctggtta acggcgtaca agtcgccaat
     1801 ccgcgcgtaa ttgccaaagc aggtttggaa tgggatacac cgtttgccaa aggattgact
     1861 ttaaacggta atgtttcgta tttcggcaag tcttaccaag acacgcaaaa acaatacgcc
     1921 ttcccgtcct acaccttgat tgacgtaggc gcgcgctaca aaaccaagct cggcaaaaat
     1981 actttgaccg tcagcagctc ggtggaaaac ctgttcaaca aaaactattg gcaggtacag
     2041 cgcggccaat acgaccgcag cttcgccgtc gtcggtatgc cgcgcactta ctggctgaag
     2101 gcggaattgg atttctgatt tccctatatc cagaaaatgc cgtccgaaca ggtttcagac
     2161 ggcatttctt ttaaagagat atggcgggat acgcttaaat aatgctattc tagcgtcttt
     2221 gaaacctgct tgtttgaagt gatgaaaaaa cctttgattt cggttgcggc agcattgctc
     2281 ggcgttgctt tgggcacgcc ttattatttg ggtgtcaaag ccgaagaaag cttgacgcag
     2341 cagcaaaaaa tattgcagga aacgggcttc ttgaccgtcg aatcgcacca atatgagcgc
     2401 ggctggttta cctctatgga aacgacggtc atccgtctga aacccgagtt gctgaataat
     2461 gcccgaaaat acctgccgga taacctgaaa acagtgttgg aacagccggt tacgctggtt
     2521 aaccatatca cgcacggccc ttttgccggc ggatttggca cgcaggcgca cattgaaacc
     2581 gagttcaaat acgcgcctga aacggaaaaa gttttggaac gcttttttgg gaaacaagct
     2641 ccggtttccc ttgccaatac cgtttatttt aacggcagcg gtaaaatgga agtcagtgtt
     2701 cccgccttcg attatgaaga gctgtcgggc atcaggctgc attgggaagg attgacggga
     2761 aaaacggttt atcaaaaagg tttcaaaagc taccgcaaca gctatgatgc gcccttgttc
     2821 aaaatcaagc tggcagacaa aggcgatgcc gcgtttgaaa aagcgcattt cgattcggaa
     2881 acttcagacg gcatcaatcc gcttgctttg ggcagcagca atctgacctt ggaaaaattc
     2941 tccctagaat ggaaagaggg tgtcgattac aacgtcaagt taaacgaact ggtcaatctt
     3001 gttaccgatt tgcagattgg cgcgtttatc aatcccaacg gcagcatcgc accttccaaa
     3061 atcgaagtcg gcaaactggc tttttcaacc aagaccgggg aatcaggctc atttatcaac
     3121 agtgaagggc agttccgttt tgacacgctg gtgtacggca atgaaaaata cggcccgctg
     3181 gacatccata tcgccgccga acacctcgat gcttctgcct taaccgtatt gaaacgcaag
     3241 tttgcacaaa tttccgccaa aaaaatgacc gaggagcaaa tccgcaatga tttgattgcc
     3301 gccgtcaaag gcgaggcttc tggactgttc accaacaatc ccgtattgga cattaaaact
     3361 tttcgtttca cgctgccgtc aggaaaaatc gatgtgggcg gaaaaatcat gtttaaagac
     3421 atgaagaagg aagatttgaa ccaattgggt ttgatgctga agaaaaccga agccgacatc
     3481 agaatgagta ttcctcaaaa aatgttggaa gacttggcgg tcagtcaggc aggcaatatt
     3541 ttcagcgtca atgccgaaga tgaggcggaa ggcagggcga gtcttgacga catcaacgag
     3601 accttgcgcc tgatggtgga cagtacggtt cagagtatgg caagggaaaa atatctgact
     3661 ttgaacggcg accagattga tactgccatt tctctgaaaa acaatcagtt gaaattgaac
     3721 ggtaaaacgt tgcaaaacga accggagccg gattttgatg aaggcggtat ggtttcagag
     3781 ccgcagcagt aatgggtcaa atgccgtctg aaatgttcag gcggcatttt gttgtattgg
     3841 aaactgccgt tttcaaccat tcctttagat aagccgtgtc ggattc
//
LOCUS       DAXHKX010000176         3880 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHKX010000176 DAXHKX010000000
VERSION     DAXHKX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.176"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            354..3602
                     /gene="nalP"
                     /locus_tag="ACFMWZ_001864"
     CDS             354..3602
                     /gene="nalP"
                     /locus_tag="ACFMWZ_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HFC5935771.1"
                     /translation="MRTTPTFPTKTFKPTAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLH
                     TGDFPNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYN
                     ENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDLVSHII
                     GGRSVDGRPAGGIAPDATLHIMNTNDGTKNEMMVAAIRNAWVKLGERGVRIVNNSFGT
                     TSRAGTADLFQIANSEEQYRQALLDYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLF
                     IFSTGNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFKREMYGEPGTEPLEYG
                     SNHCGITAMWCLSAPYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDN
                     LRTTLLTTAQDIGAVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFR
                     NDISGTGGLIKKGGSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNG
                     AASGGSLNSDGIVYLADTDQSGANETVHIKGSLQLDGKGTLYTRLGKLLKVDGTAIIG
                     GKLYMSARGKGAGYLNSTGRRVPFLSAAKIGQDYSFFTNIETDGGLLASLDSVEKTAG
                     SEGDTLSYYVRRGNAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPE
                     TVETAAADRTDMPGIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADM
                     QGRRLKAVSDGLDHNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENT
                     TAAATLGMGRSTWSENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRST
                     GADEHAEGSVNGTLMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGW
                     SGNSLTEGTLVGLAGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGK
                     TGARNMPHTRLVAGLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1049 a         1047 c         1032 g          752 t
ORIGIN      
        1 atagtacgga accgattcac ttgttaaaga atcgttctct ttgagctaag gcgaggcaac
       61 gccgtactgg tttttgttca tccactataa ctagggaaat tcaaattaag ttagaattat
      121 ccctatgaga aaaagccgtc taagccggta taaacaaaat aaactcattg agctatttgt
      181 cgaaagttca aatttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttgtcca
      241 agacaacccc tataataata taattcaaaa tataaaaatg ggttacatct aaacattacg
      301 gaatttttat tccctcgcct gaattctatt gtcagattca aggagacctc atcatgcgaa
      361 cgaccccaac cttccctaca aaaactttca aaccgactgc catggcgtta gctgttgcaa
      421 caacactttc tgcctgctta ggcggcggcg gaggcggcac ttctgcgccc gacttcaatg
      481 caggcggtac cggtatcggc agcaacagca gagcaacaac agcgaaatca gcagcagtat
      541 cttacgccgg tatcaagaac gaaatgtgca aagacagaag catgctctgt gccggtcggg
      601 atgacgttgc ggttacagac agggatgcca aaatcaatgc cccccccccg aatctgcata
      661 ccggagactt tccaaaccca aatgacgcat acaagaattt gatcaacctc aaacctgcaa
      721 ttgaagcagg ctatacagga cgcggggtag aggtaggtat cgtcgacaca ggcgaatccg
      781 tcggcagcat atcctttccc gaactgtatg gcagaaaaga acacggctat aacgaaaatt
      841 acaaaaacta tacggcgtat atgcggaagg aagcgcctga agacggaggc ggtaaagaca
      901 ttgaagcttc tttcgacgat gaggccgtta tagagactga agcaaagccg acggatatcc
      961 gccacgtaaa agaaatcgga cacatcgatt tggtctccca tattattggc gggcgttccg
     1021 tggacggcag acctgcaggc ggtattgcgc ccgatgcgac gctacacata atgaatacga
     1081 atgatggaac caagaacgaa atgatggttg cagccatccg caatgcatgg gtcaagctgg
     1141 gcgaacgtgg cgtgcgcatc gtcaataaca gttttggaac aacatcgagg gcaggcactg
     1201 ccgacctttt ccaaatagcc aattcggagg agcagtaccg ccaagcgttg ctcgactatt
     1261 ccggcggtga taaaacagac gagggtatcc gcctgatgca acagagcgat tacggcaacc
     1321 tgtcctacca catccgtaat aaaaacatgc ttttcatctt ttcgacaggc aatgacgcac
     1381 aagctcagcc caacacactg accctattgc cattttatga aaaagacgct caaaaaggca
     1441 ttatcacagt cgcaggcgta gaccgcagtg gagaaaagtt caaacgggaa atgtatggag
     1501 aaccgggtac agaaccgctt gagtatggct ccaaccattg cggaattact gccatgtggt
     1561 gcctgtcggc accctatgaa gcaagcgtcc gtttcacccg tacaaacccg attcaaattg
     1621 ccggaacatc cttttccgca cccatcgtaa ccggcacggc ggctctgctg ctgcagaaat
     1681 acccgtggat gagcaacgac aacctgcgta ccacgttgct gacgacggct caggacatcg
     1741 gtgcagtcgg cgtggacagc aagttcggct ggggactgct ggatgcgggt aaggccatga
     1801 acggacccgc gtcctttccg ttcggcgact ttaccgccga tacgaaaggt acatccgata
     1861 ttgcctactc cttccgtaac gacatttcag gcacgggcgg cctgatcaaa aaaggcggca
     1921 gccaactgca actgcacggc aacaacacct atacgggcaa aaccattatc gaaggcggtt
     1981 cgctggtgtt gtacggcaac aacaaatcgg atatgcgcgt cgaaaccaaa ggtgcgctga
     2041 tttataacgg ggcggcatcc ggcggcagcc tgaacagcga cggcattgtc tatctggcag
     2101 ataccgacca atccggcgca aacgaaaccg tacacatcaa aggcagtctg cagctggacg
     2161 gcaaaggtac gctgtacaca cgtttgggca aactgctgaa agtggacggt acggcgatta
     2221 tcggcggcaa gctgtacatg tcggcacgcg gcaagggggc aggctatctc aacagtaccg
     2281 gacgacgtgt tcccttcctg agtgccgcca aaatcgggca ggattattct ttcttcacaa
     2341 acatcgaaac cgacggcggc ctgctggctt ccctcgacag cgtcgaaaaa acagcgggca
     2401 gtgaaggcga cacgctgtcc tattatgtcc gtcgcggcaa tgcggcacgg actgcttcgg
     2461 cagcggcaca ttccgcgccc gccggtctga aacacgccgt agaacagggc ggcagcaatc
     2521 tggaaaacct gatggtcgaa ctggatgcct ccgaatcatc cgcaacaccc gagacggttg
     2581 aaactgcggc agccgaccgc acagatatgc cgggcatccg cccctacggc gcaactttcc
     2641 gcgcagcggc agccgtacag catgcgaatg ccgccgacgg tgtacgcatc ttcaacagtc
     2701 tcgccgctac cgtctatgcc gacagtaccg ccgcccatgc cgatatgcag ggacgccgcc
     2761 tgaaagccgt atcggacggg ttggaccaca acggcacggg tctgcgcgtc atcgcgcaaa
     2821 cccaacagga cggtggaacg tgggaacagg gcggtgttga aggcaaaatg cgcggcagta
     2881 cccaaaccgt cggcattgcc gcgaaaaccg gcgaaaatac gacagcagcc gccacactgg
     2941 gcatgggacg cagcacatgg agcgaaaaca gtgcaaatgc aaaaaccgac agcattagtc
     3001 tgtttgcagg catacggcac gatgcgggcg atatcggcta tctcaaaggc ctgttctcct
     3061 acggacgcta caaaaacagc atcagccgca gcaccggtgc ggacgaacat gcggaaggca
     3121 gcgtcaacgg cacgctgatg cagctgggcg cactgggcgg tgtcaacgtt ccgtttgccg
     3181 caacgggaga tttgacggtc gaaggcggtc tgcgctacga cctgctcaaa caggatgcat
     3241 tcgccgaaaa aggcagtgct ttgggctgga gcggcaacag cctcactgaa ggcacgctgg
     3301 tcggactcgc gggtctgaag ctgtcgcaac ccttgagcga taaagccgtc ctgtttgcaa
     3361 cggcgggcgt ggaacgcgac ctgaacggac gcgactacac ggtaacgggc ggctttaccg
     3421 gcgcgactgc agcaaccggc aagacggggg cacgcaatat gccgcacacc cgcctggttg
     3481 ccggtctggg cgcggatgtc gaattcggca acggctggaa cggcttggca cgttacagct
     3541 acgccggttc caaacagtac ggcaaccaca gcggacgagt cggcgtaggc taccggttct
     3601 gacggacagg aagcagacag ccgcaaagat cacggtcttt gcggctgttt cttatgaaaa
     3661 gaaaacccta ttccaattgc ctgcttctat tgtttcaaga cttcttccaa agattcggca
     3721 ttaatcagat gtatagcgga ttaacaaaaa tcaggacaag gcggcgaagc cgcagacagt
     3781 acagaaccga ttcactcggt gcttcagcac cttagagaat cgtttctttg agctaaggcg
     3841 aggcaacgct gtactggttt ttgttaatcc gctatattcc
//
LOCUS       DAXHKX010000177         3849 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHKX010000177 DAXHKX010000000
VERSION     DAXHKX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.177"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            479..1435
                     /gene="thiL"
                     /locus_tag="ACFMWZ_001865"
     CDS             479..1435
                     /gene="thiL"
                     /locus_tag="ACFMWZ_001865"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC5935772.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1428..1913
                     /locus_tag="ACFMWZ_001866"
     CDS             1428..1913
                     /locus_tag="ACFMWZ_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC5935773.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1973..3649)
                     /gene="ettA"
                     /locus_tag="ACFMWZ_001867"
     CDS             complement(1973..3649)
                     /gene="ettA"
                     /locus_tag="ACFMWZ_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC5935774.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDGLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          782 a          942 c         1072 g         1053 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatatcg gttgaaactc tgattttaag gcggtaggat
       61 gtgggtttgc ccatagaaag ggaatccttt ctgtatcaag ccctgaaagg gataattcat
      121 acaaattcac gcctttcccc ttcattggga aatggatgga atcgtgccag atgtgtgcgg
      181 cactgtatgc cggatatggt tttatcatca gcccttttcg gttgaaaccc cgtcagttgc
      241 agcgattgag cctaatcggt ggcggaagtt gccgctttgc attcggggtg gcgtgcagtg
      301 cggtgctttg atatgccgtt tgtgtgttga aacagggtgg tcggtgcata cgggtacggt
      361 atggccaaag ctaaaagtga aatacgctga aacactgaat gagccgcttt attgtttgta
      421 cggcctttgc tgccttgcta tgatttaaat tggattcgcc cgccggatat tttgggatat
      481 gaaagaattt gacttcatca aacggtattt gcaaacaggc acggataagg atgtcgtatt
      541 gggcataggc gacgatgcgg cgattgtccg cccgcgtgaa ggcttcgatt tgtgtttcag
      601 tgcggatatg cttttgaagg acaggcattt ttttgccgat gtcaaacctg aggacttggc
      661 ttggaaggtt ttggccgtca atatttcaga tatggcggcg atgggcgcga taccgcgttg
      721 ggtgttgtta agtgcggctt tgcccgaatt ggatgaggta tggctggaac ggttttgcgg
      781 cagctttttc ggtttggcaa aaaagtttgg cgtaacgtta atcggcggcg atacgaccaa
      841 gggcgatatg gcgttcaatg taaccattat cggcgaattg ccgaagggta gggcattgcg
      901 gcgtgatgcg gcggttgcgg gcgacgatat ttgggtgtcg gggcgtgtcg gtatggcggc
      961 ggcggctttg aactgccgtc tgaaacggtg tgtgttgccg gatgatgtgt ttgccgaatg
     1021 cgaacaaaag ctgctccgcc ccgagccaag ggttgggctg gggcttgcgc tgttgccgtt
     1081 tgcccgggcg gcgcaggatg tttcagacgg cctcgcgcaa gatttggggc atatcctgac
     1141 cgcttccggc gtgggggcgg aaatttgggc cgattcgctg ccgtctttat ccgtattgaa
     1201 agatattttg ccccgagcgc aatggctgtc ttatactttg gcgggcggcg acgattacga
     1261 gctggtgttt accgcgccgg aaagttgccg cagccgcgtg cttgatgcgg cagaacggtg
     1321 cggcgtgccg gtaacgcgca tcggcaaaat caacggagga tgccgtctga aggttttaga
     1381 tgccgacggc agggaattgg aactacattc tttaggattc gatcattttg gctgatttta
     1441 aacctgactt tgcgtggctg ttgaaacgac cgttgtgttt tttggctttt ggtttcggca
     1501 gcgggctggc tccgttcgcg ccgggtacat ttggcacttt ggcggcactg cctttggcat
     1561 ttgtgctgat tttgctcggc atagacgggc tgctgctggc ttttttgtgt atcgtgctgt
     1621 ttatgtgggg catacgcatt tgcgcttatg cggaacgtga aacgggtgtc agcgaccatg
     1681 gtgggattgt ttgggacgag attgtcgcca tgctgtttgt gctggcgttt gtgccgttca
     1741 ggtggacgtg gtggctggcg gcatttgtcc tattccgtct gtttgacgcg ctcaaaccgt
     1801 ctcccgtcgg ttggtttgac aagaatctgc acggcggttt gggcattatg gcggacgata
     1861 tggcggctgc ggtgatgact ttgattgtct tgaggattgc aatgctgttt taaacggtgc
     1921 tgccttgtaa aaatgccgcc tgaaagcctt tcagacggca ttgtttcgga ggttaacgtg
     1981 ttaccggttt atatttaatc cgtttcggtt tcgcgccttc ttcgccgagt cggcgtttct
     2041 tatcggcttc gtattcctga tagttgccgt caaagaacac ccatttggag tcgccttcgc
     2101 aagccaagat atgcgtagca atacggtcga ggaaccagcg gtcgtgcgaa atcaccatca
     2161 cgctgccggc aaattccagc aatgcgtctt ccaacgcgcg cagggtttcc acgtcgaggt
     2221 cgttggacgg ttcgtccagc agcaacacat tgccaccgcc caacaaggtt tttgccaagt
     2281 gcaaacgtcc gcgttcgccg ccggaaagct gccccgtgat tttgctttgg tcgctgcctt
     2341 tgaaattgaa gcgtcccaaa tattggcggg cggggatttc aaactgcccg acctgtaaaa
     2401 tatcgcgacc ttcggcaatg ttgtcgaaca cggttttgtc gttttgcaaa ccttcgcggc
     2461 tttggtcaat caagctcatt ttcacggttt gcccgatttt cacttcaccg gaatcgggct
     2521 gctctttgcc cgcaatcatt ttaaacagcg tcgatttacc cgcgccgttc ggaccaatga
     2581 tgccgacaat cgcgcccgca ggcactttga agctcaaacc gtcaatcagc actttgtcgc
     2641 cgaacgattt ggaaacattc acaaattcaa tcacttcgtt acccaaacgc tcggcgacgg
     2701 gaatgaagat ttcctgcgtt tcattgcgtt tttggtattc gtagttgctc atttcttcaa
     2761 aacgagccaa acgcgctttg gacttggctt ggcggccttt ggcattttgg cgcacccatt
     2821 ccaattcctg cttcatcgcc ttcacgcgcg cggcttcgga ttttgcctcg ttttccaagc
     2881 gtttttcttt ctgctccagc caagacgagt aattgccttt ccacggaata ccatggccgc
     2941 ggtcgagttc caaaatccat tcggcggcgt tgtcgaggaa gtagcggtcg tgtgttaccg
     3001 caacgactgt accggggaag cgcacgagaa attgctccag ccactcgaca gattccgcgt
     3061 ccaagtggtt ggtcggctcg tccagcaaaa gcatatcggg tttgctcaac aagagtttgc
     3121 acaaggcaac gcggcgtttt tcaccgccgg acaaattatc gattttggca tcccattccg
     3181 gcaggcgcag cgcgtcggcg gcgatttcca attcgtgttc cgcaccgccg cccgtggacg
     3241 aacctgccgc aataatcgct tccaagcggc cctgctcttc tgccaacgcg tcaaaatccg
     3301 catcaggatt ggcgtactcg gcatacactt cttccaaacg tttctgcgcg gcagccactt
     3361 cgcccaaacc gctttccact tcctcgcgca cggttttttc aggatcaagc tcaggctctt
     3421 gcggcaggta gccgatttta atgccgccca tcggcacggc ttcgccctca aattccttat
     3481 ccacacccgc cataatccgc agcacggtgg acttgcccgc gccgttcaaa ccgagcaggc
     3541 cgattttcgc gccggggaag aaagaaaggg aaatatcttt aatgatggtt ttctgcggcg
     3601 gcacaacctt gcttacgcgc agcatagaat agacgtattg ttgggacatg gttttctcgt
     3661 tttcatcaaa caaatttcag acggccattt taaccgataa tttgatttaa gccagtttat
     3721 ccgcgaaccg gtattgccaa aatcgggcag gattcataaa atccgcttat ccctttgaaa
     3781 ttatatagac aaaaaaaata ataatgatag gggatcgccg ccccggcaac catttcggat
     3841 ttcccaaag
//
LOCUS       DAXHKX010000178         3838 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHKX010000178 DAXHKX010000000
VERSION     DAXHKX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.178"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..1547
                     /locus_tag="ACFMWZ_001868"
     CDS             <1..1547
                     /locus_tag="ACFMWZ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC5935775.1"
                     /translation="VSNDPKPKKQPAPPKGRLKPLPLADIRTFQAWIKTAERDNLRLL
                     FLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRL
                     HAPSLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITQFLSQNPDRNAPILPQLA
                     QAIAKTAGDIRILEQIDQDAWAAQELPEHCYLNLRIIDDGGQELAGGRKLHELQQQLG
                     QAAAVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRL
                     FDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQ
                     AVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGK
                     HPLTHLLRLRLQTLLAPGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREAD
                     IQELEQMWQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLK
                     EWEKIF"
     gene            1614..2444
                     /locus_tag="ACFMWZ_001869"
     CDS             1614..2444
                     /locus_tag="ACFMWZ_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC5935776.1"
                     /translation="MSLENFQLIKTQLGVPSYENDRVAIYQGDCIQLMDKISKGSFQM
                     TITSPPYNIGKEYESILDLEHYLEWCERWMQSIHHLTEDSGCFWLNLGYLHVEKKGLA
                     VPIPYLLWDKTNFFLLQEVVWNYAAGVACRNRFSPRNEKLLWYVKNPAKYTFNLDAVR
                     DPNVKYPNQKKNGKLKCNPLGKNPTDVWQIAKVTSGRNRSSRERTAHPAQFPLELIER
                     IIRASSNQNDVILDPFIGSGTTALAGLKNNRFVIGFEINDEYIDIIKERFKEYDLQNT
                     "
     gene            complement(2501..3100)
                     /locus_tag="ACFMWZ_001870"
     CDS             complement(2501..3100)
                     /locus_tag="ACFMWZ_001870"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScaI family restriction endonuclease"
                     /protein_id="HFC5935777.1"
                     /translation="MNRSKISPYASLNEELWISKTEELIKQHPLSEKEIVQLCLDSWE
                     NIFSSRIGNLQIGKNFFPSPQIVGALLHALIPANIEARFENWQGEKNKYDKDIVYIKD
                     DYYSIELKTSSDPKYIFGNRSYAQPQDGITGKSKNGYYLTINFEKFMPDLKLRPEIKI
                     IRFGWLDHTDWIAQTAATGQQARLSPEAYRSKLKILYQK"
     gene            complement(3102..3317)
                     /locus_tag="ACFMWZ_001871"
     CDS             complement(3102..3317)
                     /locus_tag="ACFMWZ_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC5935778.1"
                     /translation="MENELVEFGKRVKHYRLLNGWSQEELAEKVGLHRTYIGSVERGE
                     RNISLLNISLLAKSFSINISELVEGIK"
BASE COUNT         1159 a         1007 c          707 g          965 t
ORIGIN      
        1 cggtttcaaa cgaccccaaa cccaaaaagc agcctgcacc cccaaaaggc cgtctgaaac
       61 ccctccccct cgccgacatc cgtaccttcc aagcctggat caaaaccgcc gaacgcgaca
      121 atctgcgcct gctgttcctc agccgcgacg atttgatgca acacgccgcc gcacacatta
      181 ccgaagagca attccccaaa ttctggcaaa ccgcagacgg caagttcaaa ctttcctacc
      241 gcttcgagcc gcaccatccg ctcgacggcg ttaccctcac cctaccgctg accgtcctca
      301 accgcctgca cgcgccgtcg ctcgaatggc tggtgcccgg tatgttgcgc gaaaaaatcc
      361 agctgctgat taaagctctg ccgaagcaaa tccgccgcat ttgcgtgccc gtacccgatt
      421 tcatcaccca atttttaagc caaaaccctg atcgcaacgc ccccatcctg ccccaacttg
      481 cccaagccat cgccaaaacc gcaggcgaca tccgcatact cgagcaaatc gaccaagacg
      541 catgggcggc gcaagaattg cccgaacact gctatctgaa tctgcgcatt atcgacgacg
      601 gcggacaaga gcttgccggc ggccgcaaac tgcacgaatt gcaacaacag ctcggccaag
      661 ccgccgccgt caccttccgc gacaacaccc aagaattcga gcgcgacaac gtgaccacat
      721 gggacatcgg aaccctgccc gaatccatca aattcgcccg cggcaaacaa cagctcaccg
      781 gctacctcgg cctgcaaaaa gaaaaagacg gccgcatcgc cctgcgcctg tttgacacca
      841 ccgaagccgc cgaacaggca caccgtcaag gcgtcatcga attgatgaag ctgcaattaa
      901 aagagcaggt aaaagacctg aacaaaggca tccaaggctt tacccaagcc gccatgctgc
      961 tcaaacacat caacgccgac accctgcgcg acgacctcac ccaagccgtc tgcgaccgcg
     1021 cctttatcgg cgaagacgag ctgccgcgca acgaaaaagc cttcaaagaa caaatcaaac
     1081 gcgcccgtag ccgcctgccc gccgtcaaag aagccctcag ccgctacctg caggaaaccg
     1141 cagccgccta cgccgaactc aacggcaaac tcggcaaaca tccattgacc cacctcctaa
     1201 gactacgcct gcaaaccctg cttgcccccg gcttcgccac ccgcaccccg tgggcacaat
     1261 ggccgcgcct ccccatctac ctcaaagcca tgaccctgcg cctcgaaaaa tacagcagca
     1321 accccgcccg cgacgcagcc cgcgaagccg atatccaaga gctggaacaa atgtggcagg
     1381 aaaaaacaga cagcctgatt aaacaaggtc tccccatttc agacggcctc gccgcgttta
     1441 aatggatgat tgaagaattg agggtgtcgc tgttcgcgca ggaattgaag acaccgtatc
     1501 cggtgtcggt gaagcggttg ttgaaagagt gggagaaaat tttttaactg aataattctg
     1561 ccgatcaaaa tttcttgaca aaacaaattt taatgaatac tataagtagc attatgagtt
     1621 tagaaaattt tcaattaata aaaacacaat taggtgttcc cagttatgaa aatgatagag
     1681 ttgcaatcta tcaaggagat tgtatccagt taatggataa gatttcaaaa ggatcattcc
     1741 aaatgacaat cacatctcca ccatacaata ttggtaaaga atatgagtct attttagatt
     1801 tggaacatta tcttgagtgg tgtgaaagat ggatgcaatc aatacatcac ttaactgaag
     1861 atagcggatg tttttggtta aatttaggat acttacatgt tgaaaaaaag ggactagcag
     1921 ttcctattcc ttatctttta tgggataaaa ctaacttttt ccttttacaa gaggtggttt
     1981 ggaattatgc agctggagtt gcatgccgta atcgattttc cccaagaaat gaaaagttat
     2041 tatggtatgt gaaaaatcct gctaaatata cattcaattt agatgctgta cgcgatccca
     2101 acgttaaata tccaaatcag aaaaaaaatg ggaaattaaa atgtaatcct ctaggaaaaa
     2161 acccaacgga tgtttggcaa atagcaaaag tcacttctgg aagaaatagg tcatcgagag
     2221 aaagaactgc tcatcctgcc caatttccat tggaattaat agaaagaata atccgagcaa
     2281 gtagcaatca aaatgatgta atcttagatc catttatcgg ctcaggaaca accgccttag
     2341 ccggactaaa aaacaaccgt tttgtcatag gatttgaaat taacgatgag tatatagata
     2401 ttataaaaga acgcttcaaa gagtatgatc ttcaaaatac ttgatacttg tatataacgg
     2461 tatagaggat agaccctcct ctatactgta tttacaatat ttatttttga taaagtattt
     2521 ttagtttact tctataagcc tctggagaga gcctcgcctg ctggcctgtc gctgcagttt
     2581 gagcaatcca atccgtatga tctaaccaac caaatctgat aattttaatt tctggacgca
     2641 attttaaatc aggcataaat ttttcaaaat ttatagttag ataataacca tttttactct
     2701 taccagtaat tccatcttgt ggttgtgcat aactcctatt accaaatata tattttggat
     2761 ccgatgaagt ttttaattct attgagtaat agtcgtcttt aatatataca atatctttgt
     2821 catatttatt cttttctcct tgccaatttt caaatctagc ctcaatatta gcaggaatta
     2881 aagcatgtaa taacgcccct acaatctgag gagaaggaaa aaaatttttg ccaatttgca
     2941 aattaccaat tctagacgag aaaatatttt cccaactatc taaacaaagt tggacaatct
     3001 ctttttctga caatggatgc tgcttaatca gctcttctgt tttggaaatc cataactcct
     3061 cattcaaaga agcatacggc gatatttttg atctattcat attatttaat cccttctaca
     3121 agttcagaaa tattaattga aaaagactta gctaacagcg agatattcaa aagacttata
     3181 tttctttctc cgcgttcaac tgatccaata taagtacgat gtaatccaac tttttctgct
     3241 aattcttctt gtgaccaacc attaagtaaa cgataatgtt ttacacgctt accaaactca
     3301 actaattcat tttccataat ctgtattatt ttaataagtg aatactttaa gtctacactc
     3361 tataagtagc acacttaaca aaccatatcc tactgcgtga ataccatagc aaactgtagc
     3421 ctgcatcaaa ccgcgtgcat gccttcgggc acacactcgg tctttctaaa ccactccccc
     3481 tttccaccca aaccaaaggt cgtctgaaac ccattttcag acgacctttt gccactttgc
     3541 aaagcaagta ttcccaccac cctctcccca aatatcgccc aaaccagtaa acttcccata
     3601 tttcaacaac ttcttgaaag ccaaccatgt ccggtatcta cctaccccac ctatttccgc
     3661 cccatatcgc cgaacgcggc cttttgtatt ttcagcaggg caaggttcgc gatgtccgaa
     3721 aaacttccgc cgggcgttat cgggcctggc gctgtttgct gccggcggtc tgaataatgg
     3781 tggcgtcaac gacggcagcg gatgctttct ctacttttag gtttttttcg gtcagttg
//
LOCUS       DAXHKX010000179         3764 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHKX010000179 DAXHKX010000000
VERSION     DAXHKX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.179"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(88..906)
                     /locus_tag="ACFMWZ_001872"
     CDS             complement(88..906)
                     /locus_tag="ACFMWZ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC5935779.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTGLSDRLPLSVLTGSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(973..2223)
                     /locus_tag="ACFMWZ_001873"
     CDS             complement(973..2223)
                     /locus_tag="ACFMWZ_001873"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC5935780.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="ACFMWZ_001874"
     CDS             complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="ACFMWZ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC5935781.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            2883..3695
                     /gene="murI"
                     /locus_tag="ACFMWZ_001875"
     CDS             2883..3695
                     /gene="murI"
                     /locus_tag="ACFMWZ_001875"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC5935782.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          834 a          914 c         1122 g          894 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcgggaaa tatccatatc
      121 aggctgggga ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactat atacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gcccgttaaa acagataaag gaagccgatc ggacaatccg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacta
      961 ccgccttttc aatcaacccc gcttcaatac ggatgtattg atgtagcgtt ggacacccga
     1021 ggcaatggat tgggcgcact gccggcggaa ggattcgctg cccagcaact tctcttcagc
     1081 agggttggac aggaaggcgg tttcgaccag gatagacggc atatcgggtg cgcgcaaaac
     1141 ggcgaaattg gcttcgtcca ccctgccttt gtgcaagtgg ttgagcctgc ccaattcttc
     1201 aagcaccagt ttgccgagtt tgcggctgtc gcgcagcgtg gcggtttggg tcatatcgag
     1261 cagggcggta tcgacgttgc ggttgccgct ggtcggtacg ccgccgaccg cgtcggcatt
     1321 gttttgcgtc tgttccaaga atttggcggc ggagctggtc gcgcctttgg tgttcagcat
     1381 atagaccccc gttccgcgcg cggaggggct ggtgaaagcg tcggcgtgga tggagacgaa
     1441 tacgtccgcc cgccgtgctc gccctttggc gacacgcacg cccaatggga tgaacacgtc
     1501 ttcgttgcgc gtcataaaga cgttgtaacc caaggcttcc aattgttttt tggtttcgcg
     1561 ggcgatggag aggacgacgt gtttttcctg caaaccgccc gggctgacgg cgccggggtc
     1621 ttcgccgccg tgtccggggt cgatcataat gacgggtctg cgcccgtttt tgccgcgttc
     1681 ggtttgtgcg ggagtgtttt gagcgaggtc ggcttcaggg gagccgcgac gggttttgtt
     1741 caggctgccg ttaagcagcg ccatcatcgg atcgtcggga tccatccctt gcggatagag
     1801 atcgacgacg aggcggtttt taaagccgcc gacggggggg agggaaaaaa cttgcgcgta
     1861 ggaaggctgt ttcaaatcga tgacgaggcg gacggtgtcg ggcgtgttct gaccggcgcg
     1921 tatattgcgg ataaaggggt cgtccgccat aaccttctga gacagtccgt gcaatacggt
     1981 attgatgttc gcgttttgta tgtcgacgac caacctgccc gggttgtcga gcgtgaagtg
     2041 ttggtatttg agcgcggttg tgctttccag cgtcaggcgg gtgtaggtgt gcgacggcca
     2101 tatccgtacg gcggtaaact gcggggcgcg taccgttttg gcaacggcgg atgcgatggg
     2161 gcttagggcg aacagtgtgc cggcggtgcg gcggatgatt tgtcttcgtg tcagtttggt
     2221 catagcggca ggctttcgcg tcctcgttcg gtatgggcgg tcagcaggca ttttctgccg
     2281 ccgccgtcgt gtgtcaatgt tgcggtgatg tcggcgggcg gggtaaattc gcctccctgt
     2341 tgcggccatt cgatcaggca gacgctgttt gcggcaaaca gttcgtcaag ccccgcgtct
     2401 tcccattctt cggggaacga gaagcggtag aggtcgaaat ggtgcagggt gaagcgttcc
     2461 agcggataag attcgacgat ggcgtaggtc gggcttttga ctgcgccctg atgacccaat
     2521 ccgcgcagga tgccgcgtgt cagcgtggtt ttgcccgcac ccaaatcccc ttcgagataa
     2581 atgaccagcg gtgcgtttaa acgggaagac cacgccgcgc ccaaatcgag tgtggcggct
     2641 tcgtcggcaa ggaatcggga gatagagggt aaatcagaca tggaaacggt ttgttgtaag
     2701 gtctagggta ttatgggcag ttttgcaggt tttgcaaact ttgcacccga ggggcggatg
     2761 cttcttgtcc gagcattata acagccaaat ccgcatcctg ctttcagacg gcaacggctg
     2821 tcaagaaaaa gcggcacgtg tacaatacgc ggattgtatg tttaggacag attggaaaaa
     2881 ggatggaaaa tatcggcagg cagcgaccca tcggcgtttt tgactcggga atcggcggtt
     2941 tgaccaatgt gcgcgcactg atggagcggc tgccgatgga gaacatcatt tatttcggcg
     3001 acacggcgcg cgtgccttac gggacgaaat ctaaggcgac catcgaaaat ttctcgatgc
     3061 agattgtcga ttttctgctg gaacacgatg ttaaggcgat ggttatcgcg tgtaatacga
     3121 ttgcggcggt ggcggggcag aaaatccgtc aaaagacggg caatatgcct gtgttggacg
     3181 tgatttccgc cggcgcgaaa gccgcactgg caacgacgcg caacaacaaa atcggcatta
     3241 tcgccaccaa taccacagtt aacagcaacg cctatgcgcg cgccatccat aggaacaacc
     3301 ccgacacgct tgtccgcacg caggccgcgc cgctgctcgt ccctttggtg gaagagggct
     3361 ggttggaaca cgaagttacc cgcctgaccg tatgcgaata cctcaaacca ttgcttgcag
     3421 acggcatcga tacgctggtg ttgggctgca cgcactttcc cttgctcaag cccttaatcg
     3481 gcagggaggc gggcaatgtc gcgttggttg attctgcaat tacaacggcc gaagaaaccg
     3541 cacgcgtcct tgctcaggaa ggattgctca ataccgacaa caacaatccc gactaccgtt
     3601 tttacgtcag cgatattcct ttgaaattca gaaccatcgg cgagcgtttt ctgggcagga
     3661 cgatggagca gattgaaatg gtgtctttgg gttaaaacga tgacggaaag ctgcccgaga
     3721 ttacagaaac ctaaaatccc gtcattcccg cgcaggcggg aatc
//
LOCUS       DAXHKX010000180         3754 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHKX010000180 DAXHKX010000000
VERSION     DAXHKX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.180"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(214..1278)
                     /locus_tag="ACFMWZ_001876"
     CDS             complement(214..1278)
                     /locus_tag="ACFMWZ_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC5935783.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="ACFMWZ_001877"
     CDS             complement(1279..2037)
                     /locus_tag="ACFMWZ_001877"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC5935784.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="ACFMWZ_001878"
     tRNA            complement(2112..2187)
                     /locus_tag="ACFMWZ_001878"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3377)
                     /locus_tag="ACFMWZ_001879"
     CDS             complement(2241..3377)
                     /locus_tag="ACFMWZ_001879"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC5935785.1"
                     /translation="MDWHERFPELEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVD
                     AAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVR
                     MMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFF
                     PSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAII
                     YAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHANLAPEDIGWINLHGTGTHHND
                     SMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPQLW
                     DGQNDPNLPAINLTGSGSRWETEKRITASSSFAFGGSNCVLIIG"
     gene            complement(3322..>3754)
                     /locus_tag="ACFMWZ_001880"
     CDS             complement(3322..>3754)
                     /locus_tag="ACFMWZ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC5935786.1"
                     /translation="ALAADEVFEGPVEQDESDFGGRRKGRRGRGAAGKVVVFGILKHN
                     GRVYTVIVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFA
                     DRQNHINGIENFWNQAKRVLRKYSGLARTFPRIGSATGCAD"
BASE COUNT          847 a          928 c          983 g          996 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac ggttggcgac gcattgatgt cctgccgttg ccagtgcgtt
      301 tttcgtttca gggttcaaga cgaggcgttc gatttcgtcc gccaaagatt caggtgtcag
      361 cctgtccagc agaatccccg attgatgatg ttcgataatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caagtttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatccggat gtccgtcccc tgccaatttg agcctgattt gaggatattt
      661 ccgatgcagt atcacacagg cttcaatcag gttttccaat cctttttctg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaacg
      781 gccggtatcg ataccgttat gaacaatccg gtattttgct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtggtaa aaatcggttt tgttggcgac gacattatgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggtttttcc ggataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgcgttg tgaaaacgga agaaacttcg gaaaaacgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat cgtgcaaccc cgaagcccca atgcttttga
     1201 aacatcatag acatactgtt cgcccccgcc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccagatttg tacagataat atagtttgac
     1321 atatttaatc atcgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 gagaaaccct ttgttcagga tataaatttt gaaaaaaccc caaatcgggc ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 gtatttgttg aatttgttga aatattgttc ccagttgtcg tatgtgtaat ggtacataaa
     1561 atgcttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct tttttcggca tcagacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ttcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgtctga agacttttgc acccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcgtcaa taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcctgaatg tggtttgctt cgtttttggc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc tccgaaggca
     2281 aacgacgagc tggcggtaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gaaggttcgg atcgttctgc ccgtcccaaa gctggggcgg aagttttcct
     2401 tcgggattgc tttgccggtc ggcaatgccc cacgcgaacg cggcttcgat tgcgcccgcc
     2461 gcacccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcacggct ttccatactg tcgttgtggt gcgtcccggt gccgtgcagg
     2581 ttaatccagc cgatgtcttc gggcgcaagg tttgcgtgtt gcaatgccgt ctgaaaggcg
     2641 aggattgcgc cttgagcgtc ggggcgcggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattatc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgggaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacgtgcc ggtcagcaac
     3121 aattcgccca catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atgcgtccgt cagtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatc ctcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccactat attttcgcaa gacgcgtttt gcctgattcc aaaaattctc
     3421 aatgccgtta atgtggttct gacggtctgc aaattccttg gaatggttga tgcggtaatg
     3481 gataaaaccg ctcacgtcca acttgtcgca gctgctcaga ctatcggtat aaacaatact
     3541 gtccggcatg attttctttt tgatgacagg gagtaacgtt tcagatttgg cattatctac
     3601 gataacggta tagacccgtc cgttgtgttt cagaatgccg aagacaacca cttttcctgc
     3661 cgcaccccga ccacgtctgc ctttacgccg tccgccgaaa tcgctttcgt cctgctcgac
     3721 agggccctca aaaacctcat cggcagccaa ggcc
//
LOCUS       DAXHKX010000181         3731 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHKX010000181 DAXHKX010000000
VERSION     DAXHKX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.181"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..412)
                     /locus_tag="ACFMWZ_001881"
     CDS             complement(<1..412)
                     /locus_tag="ACFMWZ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFC5935787.1"
                     /translation="MTHISSPRNEFIRGIKESSPMLIGLLPWALILGMQGGQKGMSWL
                     EMLLMTGMNFAGGSEFTTVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKGIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNM"
     gene            555..1007
                     /gene="dut"
                     /locus_tag="ACFMWZ_001882"
     CDS             555..1007
                     /gene="dut"
                     /locus_tag="ACFMWZ_001882"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC5935788.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFAVKPFERIAQMVIVPIVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            1085..2272
                     /gene="dapC"
                     /locus_tag="ACFMWZ_001883"
     CDS             1085..2272
                     /gene="dapC"
                     /locus_tag="ACFMWZ_001883"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC5935789.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLDPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDFQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            2535..3314
                     /gene="yaaA"
                     /locus_tag="ACFMWZ_001884"
     CDS             2535..3314
                     /gene="yaaA"
                     /locus_tag="ACFMWZ_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC5935790.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTPFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3397..3487
                     /locus_tag="ACFMWZ_001885"
     tRNA            3397..3487
                     /locus_tag="ACFMWZ_001885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3431..3433,aa:Ser,seq:tga)
     gene            3565..3654
                     /locus_tag="ACFMWZ_001886"
     tRNA            3565..3654
                     /locus_tag="ACFMWZ_001886"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3599..3601,aa:Ser,seq:cga)
BASE COUNT          966 a          886 c          918 g          961 t
ORIGIN      
        1 gcatattgaa tgcgggcaaa ccggctgctt tccgtttttg gatttcggag aatgccatcg
       61 cccagctttc atcacacata aaaaacagtg cgggcacggc ttttttcagc ggtattcctt
      121 tcaggtgcgg ggcaagcgcc gcccccatca ggatatgccg cgaattaatc ataaaggtta
      181 cggtggcgat aagcagtatc ggcagaggtt ccgcccacag gttgaccgtg gtaaattcgg
      241 agccgccggc gaagttcata ccggtcatca gcaacatctc cagccagctc atgccttttt
      301 gtccgccctg cataccgagt attaatgccc aaggcaaaag cccgatcagc atgggcgaac
      361 tttctttgat gccgcgtata aattcgttac ggggcgagct tatgtgtgtc atattaatat
      421 tgatatgtaa gaaggtcggg cattatacac agacggagta tttcaaaaca aaaccgattt
      481 gccgtgtttc agcgtaaaca cggcttgtgt ataatctccc atctttgaaa tcggccgtat
      541 gcaggagcaa gacgatgaat attgaagtag aaatgaaagt attggacgag aggatggcgg
      601 attttatccc tgcctatgca acggagggtt ctgcaggttt agatttgcgc gcctgtttgg
      661 atgaggaagt cgttttgcag ccgggtgaaa cgtttcttgt gccgacgggt ttggcaattt
      721 atttggcgaa tcccgcatat gccgccgttt tgctgccccg ttccggcttg gggcataaac
      781 acggcattgt tttgggcaat ttggtcggtt tgattgactc cgattatcaa ggggaattga
      841 aggtgtcgtt atggaacaga agcagcgaac cgtttgccgt caagccgttt gagcgtatcg
      901 cgcagatggt tatcgttcca atcgtacagg cgggcttcaa acgtgtcgag gagtttgtcg
      961 gaagcagccg gggtgagggc ggcttcggca gtacgggttc tcactaaaaa tatagaatgc
     1021 cgtctgaaag acacgtcagg ttcagacggc atatcttccg atataccgat aaccggagaa
     1081 aatcatgaat accttactca agcagctcaa accctatccc tttgcccgac tgcgcgaggc
     1141 gatgcagggc atttccgcgc ctgaaggcat ggaagccgtc cccctgcata tcggcgaacc
     1201 gaaacatcca acaccgaaag tcattacgga tgcgctgacc gcctcattgc acgagttgga
     1261 aaaatatcct ctgactgcgg gtctgcctga actgcgtcag gcgtgtgcaa actggttaaa
     1321 acgccgttac gatggcttga cagtggatgc ggataatgaa attctgccgg ttttaggcag
     1381 tagggaggcg ttgttttctt ttgttcaaac cgtgttggac cctgtttcag acggcatcaa
     1441 acccgcaatt gtcagcccga atccctttta tcagatttac gaaggtgcga cacttttggg
     1501 cggcggtgaa atccattttg ccaattgccc cgcgccgtct ttcaaccccg attggcgcag
     1561 tatttccgaa gaggtttgga aacgcaccaa actggtgttc gtctgctcac ccaacaaccc
     1621 cagcggcagc gtgctggatt tggacggctg gaaagaagtt tttgattttc aagacaaata
     1681 tggtttcatt attgcctcgg atgaatgcta ttccgaaatc tatttcgacg gtaacaaacc
     1741 tttgggctgc ctgcaggccg ctgcacagtt gggtcgaagc aggcaaaaac tgcttatgtt
     1801 caccagtttg tccaagcggt ccaatgttcc gggcctgcgt tccggttttg tcgccggcga
     1861 tgccgaactg cttaaaaact ttctgcttta cagaacctat cacggcagtg caatgagcat
     1921 tcccgtgcag cgcgcaagta ttgccgcttg ggatgacgaa cagcacgtta tcgacaaccg
     1981 ccgcctgtat caggaaaaat ttgagcgcgt tattcccatt ttgcaacagg tatttgatgt
     2041 taaattaccg gatgcctcgt tttacatctg gttgaaagtc cccgatggcg acgatttggc
     2101 atttgcacgc aatttatggc aaaaagccgc tatccaagta ttgcccggac gttttttggc
     2161 acgggatacc gaacagggca atcccgggga aggttatgtg cgtattgctt tggttgccga
     2221 tgtcgcaact tgtgtcaaag ctgcggaaac cattgtttcc ctatatcggt aaagaataaa
     2281 aaaatgccgt ctgaactttt gttcagacgg catttttcaa tattttacgg ttgaatttgc
     2341 tataacggta tttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgaagacag
     2401 tacagatagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat ccactatact
     2461 tcacttttaa tcggcttgcc cgcaaacacg tttaaactta aaatccccgt gtttgacaca
     2521 ataccgagca gattatgttt tttgtccttt cccctgcgaa gaaccttaat gaaaaagacc
     2581 ctgcccctgt cagcgagttt acccaacccg acctgctggc agagtccgac attctaatgc
     2641 agcagttgcg cgagcttgcg ccgcaacaga ttgccgaact gatgcacgtt tccgacaaaa
     2701 ttgccctctt aaacgcgcag cgcaatgcag aatggaacac gccgtttacg ccggaaaacg
     2761 ccaaacaggc ggtctttatg ttcaacggcg atgtttacga aggtatggat gcaaacacat
     2821 tggatattgg acagatacgc tatctgcaaa accatgtccg cctgctgtcc ggtctgtacg
     2881 gtcttctgcg cccgttagac ctgatacagc cttaccgcct ggaaatggga acgcccttcg
     2941 ccaatttgcg cggcaagaat ttgtatgagt tttggggcga catcattacc aaccttttaa
     3001 atgacacgct tgcccaagca ggcagcaata cgcttgtcaa ccttgcttca caggaatatt
     3061 tcaagtccgt caacacgaaa aaacttcggg cgcggctgat taccccgata tttaaagacg
     3121 aaaaaaacgg caaatataaa attatcagtt tctatgccaa gcgtgcgcgc ggattaatgg
     3181 tgcgctatgc ggcagaacat catattaccg accctgaaat gctgaaaaat tttaattacg
     3241 aaggctacgc attcaatgac gcggcttcaa atgaaagcga atgggtgttt atgcgctcgg
     3301 aacaaataaa gtgaaaacaa taaattaagt attttctgaa aaaagtgctt ggcaaaatgt
     3361 ataaacttca ttattattcc tactcttcaa gaagacggaa gcgtggcaga gcggtttaat
     3421 gcaacggtct tgaaaaccgt cgagggttga tagccctccg tgagttcgaa tctcaccgct
     3481 tccgccaatc cttggggtca aaaaccaaat aaaaatgaag tagcataaag catcggcatt
     3541 tttatttggt tttacttcat tatcggaaac gtggcagaga ggctgaatgc agcggactcg
     3601 aaatccgctg agggtgcaaa tcctccgtgg gttcgaatcc caccgtttcc gccacaaaac
     3661 aaaaccgccc tgattcgggg cggttctttt ttgttcaagt tgagaccttt gcaaaattcc
     3721 tttccctccc g
//
LOCUS       DAXHKX010000182         3719 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHKX010000182 DAXHKX010000000
VERSION     DAXHKX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.182"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(175..681)
                     /locus_tag="ACFMWZ_001887"
     CDS             complement(175..681)
                     /locus_tag="ACFMWZ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC5935791.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(684..881)
                     /locus_tag="ACFMWZ_001888"
     CDS             complement(684..881)
                     /locus_tag="ACFMWZ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935792.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(878..1144)
                     /locus_tag="ACFMWZ_001889"
     CDS             complement(878..1144)
                     /locus_tag="ACFMWZ_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935793.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(1156..1494)
                     /locus_tag="ACFMWZ_001890"
     CDS             complement(1156..1494)
                     /locus_tag="ACFMWZ_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935794.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(1491..1709)
                     /locus_tag="ACFMWZ_001891"
     CDS             complement(1491..1709)
                     /locus_tag="ACFMWZ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935795.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1722..1892
                     /locus_tag="ACFMWZ_001892"
     CDS             1722..1892
                     /locus_tag="ACFMWZ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC5935796.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(1937..2119)
                     /locus_tag="ACFMWZ_001893"
     CDS             complement(1937..2119)
                     /locus_tag="ACFMWZ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935797.1"
                     /translation="MPTPPAALMVAPVRPNPPKDGKTVTLLEHAAEFGGYVAELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(2139..2556)
                     /locus_tag="ACFMWZ_001894"
                     /pseudo
     CDS             complement(2139..2556)
                     /locus_tag="ACFMWZ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2528..2764)
                     /locus_tag="ACFMWZ_001895"
     CDS             complement(2528..2764)
                     /locus_tag="ACFMWZ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935798.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            complement(2767..2931)
                     /locus_tag="ACFMWZ_001896"
     CDS             complement(2767..2931)
                     /locus_tag="ACFMWZ_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935799.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(2934..3140)
                     /locus_tag="ACFMWZ_001897"
     CDS             complement(2934..3140)
                     /locus_tag="ACFMWZ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935800.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(3137..3694)
                     /locus_tag="ACFMWZ_001898"
     CDS             complement(3137..3694)
                     /locus_tag="ACFMWZ_001898"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC5935801.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIADAIAMELGAARV"
BASE COUNT          854 a         1073 c          826 g          966 t
ORIGIN      
        1 ggctttaatt gcggcaaaaa tagaccaagc ggagatttct gattcagaaa aaagctggtt
       61 gaaaaaagaa ttagggaaaa tcaaggacac tgcattaagt acgcttacgg aaaatgcaat
      121 taatgctatc cctgccgcca ccctcattac tcttttaaaa aaatttgttg gcctctaacc
      181 aaacttcttc tccacttccg ccccaaacgg ctccaatacc tccacaaagg caggcaaatg
      241 tttgggatgt ttttcttgca caaacgccat caagaactca atcaattcca aagccgtcgc
      301 cagttttgac gtttccggca tcacacgcgc attggccgat acggttttcg taaacgcatc
      361 ggccaggctg gccaacagct tggcgcggtc ggacgggggc aaatcttcgg tactcgaatc
      421 ctgcagcatc gtcatcgtgc tgttgtactg caccataaaa ccggccaaca tcgcacggct
      481 caagtcctca ataccgccgc cggccaaagt gtaggcggcg cgcatcttat cccagtcgtc
      541 gcctttttcc ttatccgcac gtttccacgc acgcgcagtg gtctgcggga tttcgcacat
      601 caaggccgcc gtttccaaag tttgctcgcc gctcacatag agccgacgta acttttcacg
      661 gatttcttgc gggtgagcca taattacagt cccattttcg ctttaagcaa ttcccagccg
      721 accgtaatca cgccgccgcc cagtgcgccg aatgtaatgg ccgtgcgttt cgtgtcttgg
      781 cggatttgcg caatttccgc ctgcatttct ttctgatttt tcagcgtttg atcagtcttg
      841 ttttcaatac gcgccaaggc ttctaaaatc gggtcgctca tgatttgtct gctttcctgt
      901 ccagtttttc gtttactttt tccaacttgt tttcgatgcg tcccaacgaa gccgcaatat
      961 tgtcgcggtc tgccttggca tcctgcttgg tgtggtacga gagcttgacc gcgtgcagct
     1021 cctctttcag gtcgtcgata cgcttgtccg cttctttcag acggcctgaa ataccgttga
     1081 cccaaaacca aaatgccgcc gtcgcaatcg gccacagggt tctgaaacca aattcaaagt
     1141 ccatttaaaa cccctttaaa ccggcacatc gccgaatacg atacggacgg cgtaaccgtc
     1201 agggcgattg ctggcaatct ccagtccatc accatcatta caaacacagt aatacgcccc
     1261 caaagtttgc caaaccgcac gtttaaaggt gtcatagttc gtaattggat attcaaggtg
     1321 aaacgtcgtc tgaaaatcct tatccatgcg taccgcgtac tcaatgcctg ccttatccaa
     1381 cagatcggaa acatgaatga caaacggctc ttgttcgcgt gcgcggctta agcccaattc
     1441 caaatcggca tggcgcacag ccaactgacg ttcaactaat tcacggtagg tcattctttg
     1501 atacccatta aatattttgt ccgcttgtac aatttcttaa cccacgaaat atttacaaat
     1561 gtataaacct ttgctacaac ttcgccgtca tagtgcgcat tttctcgtgc agcccgaaat
     1621 ttcgccctgg cttcttcagg gttgtccgcc caaatgctca atgaccagga cttgccgtta
     1681 aagcggtaag aaaacgtgta ctcattcata ggagaaacct tatgtatttt gaaatctata
     1741 aagacgcaaa aggcgaatac cgttggcgtt tgaaagcagc caaccatgaa atcatcgctc
     1801 agggcgaagg ctacaccagc aagcaaaact gccagcacgc agtcgatttg ctgaaaagca
     1861 ctaccgccgc tacccctgta aaagaggtat aaaatccgct ttcaccctca gcccgcgccc
     1921 tacgcgggct tttttgtcag tttccgactt tgcgggagtg attgcccgcc cagtcgcgcc
     1981 aagcctgatt ttgattttcc aactccgcca catagccgcc aaactcggcg gcgtgttcca
     2041 acagcgttac tgtcttgccg tctttcggcg gattcgggcg taccggcgca accatcaatg
     2101 cggcaggcgg tgtcggcatg accgcctttt cgacaacctt aatttccgta gccgagggcg
     2161 cgcttgtagc tgcaagccgt gagagccaaa gccgtcaata caaccgccgc ctgcattttt
     2221 acggtcttga gtaaggacat tttcgatttc ctttttattt tccgttttca gacggctgac
     2281 ttccgcctgt tttttcgcca aagccatgcc gacagcgtgc gccttgactt catatttttt
     2341 agcttccgcg cgtgccagtt ccagttcgcg cgcatagttt tgagccgaca acagcagggc
     2401 ttgcgccttg tcgcgctcca tcttgtcgat gaccgcctgc tgcttcgcaa atgccgactt
     2461 gtagccttga tggtgcgaca cagccaagcc cgtgccgaca agcgcgataa tggcaatcgg
     2521 ttgccagtta ttcgccagca gtttcacgag attcattctc gacctcctga cgcttcacgc
     2581 tgacaaatga acgcgccacc gcatagccgc cgacaatgcc caaatacacc gcccaaattt
     2641 ccgccgacgg atcgggcaac atcacaaact taaacgtccc cgccgcgcag gcaacgtttg
     2701 cccacagttt cgagtgcgac acattgcctg tcgctgggtt tttaaaaata tccaaaatac
     2761 gcattgctat tccacacttt tggtttgcag gtgccgtttc agcatttcct gataattggc
     2821 cagttcgccc tccgcaaatt caaacgccgc caagtctgcc tgttcgcttg cctcacggct
     2881 tttggccgac cacagcccaa tcatcttttc gtaaaacgca acctgtccca tgattaacga
     2941 cgattcttgc gtttgcgcgc cgcgcgtttc gcggctgcca cgcctgagtt tcttaaattt
     3001 gggcgtggat aggtgtgtat cggacgaaca tttggcatct gtataagtgc gcggtttacc
     3061 tgctcattac taggattttc tccaatcaat tttgtaagaa aataggaagc agcttctttc
     3121 aatttaccaa taatattcat actcttgccg cccccaattc catcgcaatc gcgtccgcaa
     3181 tcgcgtccgc aatcgcgcgg cagatgcccc atttagtcgt cttaaacaag gccaaatcag
     3241 tgtcgttgct gatgaaaaaa ggctcaaaca caatgccgcc ggcttgtgca taagccaggc
     3301 gcgaatgctg gcccgcattg tcgggtttaa agccgtcttc gccgcgcagt ttccagccgg
     3361 ttttcttggc aacggctttg ctcaacacct gacaccagcg tttgttttta acggtactca
     3421 aggcttcgat gcctgtcgcc gctttgctga cggcagcgtt ggtgtgaaac tcaatcgcca
     3481 catccgagcc gcgaatcagc ttgaccgctt cgcgcagcgg catattgcct ttgcccgtgc
     3541 cgtcggtttt aacagtcaaa ccgtagtcat cgcgcaaaat agcagccacg atattgcgca
     3601 tatcctgcgc caagtccgcc tcacggtcgc tgccgttgac cgcacccggg tcggtgttgc
     3661 tgtgtccagc ggttaaggtt acggttttgc ccattcatca tctccaaaaa atattgatt
//
LOCUS       DAXHKX010000183         3710 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHKX010000183 DAXHKX010000000
VERSION     DAXHKX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.183"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            269..1390
                     /gene="dnaJ"
                     /locus_tag="ACFMWZ_001899"
     CDS             269..1390
                     /gene="dnaJ"
                     /locus_tag="ACFMWZ_001899"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC5935802.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGEPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1586..3604
                     /locus_tag="ACFMWZ_001900"
     CDS             1586..3604
                     /locus_tag="ACFMWZ_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC5935803.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
BASE COUNT          785 a          834 c         1106 g          985 t
ORIGIN      
        1 aataggggga agtctgcagc cgcatcaaat gccgtctgaa catgcgttcg ggcggcgttt
       61 ttataacaaa aacacttcat ggcggttggt tttatgcctg tctaagtttt tgtgtcgtgc
      121 atacctgaag atttcagacg gcatcggttt atgctgtctg aaaagtgtat tccgtttcag
      181 tttgtaagct atggcagtct gtttgtcttg tgttttgcgc aattgccctt attttgagcc
      241 gtgattttat tttgaattag atgaaaaaat gagtaatcaa gatttttatg cgacgctggg
      301 tgtggcaaga acagctaccg atgatgagat taaaaaagcc taccggaaat tggcgatgaa
      361 ataccatccc gaccgcaatc ctgacaataa agaggcggaa gagaagttta aagaagtaca
      421 aaaggcgtat gaaactttgt ccgacaagga aaagcgcgct atgtacgacc agtatggtca
      481 tgcggcgttt gaaggcggcg gacagggggg cttcggaggg tttggcggat ttggcggtgc
      541 gcagggtttt gactttgggg atattttcag ccaaatgttt ggaggcggtt cggggcgcgc
      601 ccagcctgat tatcagggtg aggacgttca agtcggtatc gaaatcacgc ttgaagaagc
      661 cgcaaaaggt gtgaagaaac gcatcaatat tccgacttat gaagcgtgtg atgtctgtaa
      721 cggcagtggc gcgaaaccgg ggacatcccc ggaaacctgc ccgacttgca aaggttcggg
      781 tacggtgcac atccagcagg cgattttccg tatgcagcag acttgtccga cctgccacgg
      841 tgcgggcaaa cacattaaag aaccttgcgt caaatgccgt ggcgcggggc ggaataaggc
      901 ggtcaagacg gtggaagtca atattcccgc cggtatcgat gacgggcagc gtatccgttt
      961 gagcggcgaa ggcgggccgg gtatgcacgg tgagcctgcc ggcgacctgt atgtaaccgt
     1021 ccgcattcgg gcgcataaga ttttccaacg cgacggtctg gacttgcatt gcgaactgcc
     1081 gatcagtttt gccacggctg ctttgggcgg ggagttggaa gtgccgacct tggacggaaa
     1141 ggtcaagctc accgtcccca aagaaaccca aaccggcagg aggatgcgcg tgaagggtaa
     1201 gggtgtcaaa tctttacgca gcagcgcgac cggcgatttg tactgccata ttgttgtcga
     1261 aacgcctgtc aatttgaccg accgtcaaaa agagcttttg gaagaatttg agcggatttc
     1321 taccggcttg gaaaaccaaa caccgcgcaa gaaatcgttt ttagacaagc tgcgcgattt
     1381 gtttgattga ttttaaggtt cggaaacaag cagccgtatc ggggaatctc cctgatacgg
     1441 ctgtttttgt ttgtttaaaa atagttttta ttttcaatgg ggtatggggc agggtgggat
     1501 aactgttttt aactgttctt tttaaaactt gacatcatgg cgcgatgcca acaatatgtg
     1561 aacgtctgtt gtcaaaggaa gaataatgaa taaatcttta tccgggtcgg tagaagaata
     1621 ccgcgaactg acgctccgag gcatgatact cggtgcattg atcactgtaa tttttactgc
     1681 gtccaacgtt tacctcggtt tgaaagtcgg gctgaccttt gcctcgtcga ttccggcggc
     1741 ggtgatttcg atggcggttt taaagttttt caaaggcagc aatattttgg aaaacaacat
     1801 ggtgcagacc caagcttcgg cggcgggtac gctttcgacc atcatcttcg tcctgcccgg
     1861 tttactgatg gcgggctact ggagcggttt cccgttctgg cagacgacgc ttttatgtat
     1921 tgccggcggg attttggggg taatttttac cattccgctg cgttacgcga tggtggtgaa
     1981 aagcgatttg ccttatccgg aaggtgtggc ggctgctgaa attttgaaag tgggcggtca
     2041 tgaagaaggg gataaccgtc agggaggcag cggcatcaaa gagctggcgg ccggcggtgc
     2101 gttggcggga ttgatgagct tttgcgccgg aggtctgcgc gtgattgccg acagcgcgag
     2161 ttattggttt aaaagcggta cggcgatttt ccaactgccg atgggctttt cactggcatt
     2221 gttgggcgcg ggctatttgg tcggactgac gggcggtatc gccatcctgt tgggcatttc
     2281 gattgcttgg ggcattgccg tgccgtattt ctcctcacac attccgcaac cttccgatat
     2341 ggaaatggcg gcgtttgcga tgaagctgtg gaaggagaaa gtgcgtttta tcggtgcggg
     2401 gactattggc attgcggcgg tttggacgct gttgatgctg ctcaagccaa tggtggaagg
     2461 catgaagatg tcgttcaaga gttttggcgg cggtgcgccc gctacagaac gcgctgaaca
     2521 ggatttgtcg cctaaagcca tgattttctg ggtattgtct atgatgttta ttctaggcgt
     2581 gtcgttttac cactttatcg gcgattcgca cattacgggc ggcatggctt ggcttttggt
     2641 ggtcgtttgc acgcttttgg cttccgtcat cggctttttg gtcgccgccg cctgcggtta
     2701 tatggcgggt ttggtcggct cgtcttccag cccgatttcc ggcgtgggca tcgtttccat
     2761 cgtcgtgatt tcgctggttt tgctgctggt gggcgaatcc ggcggtttga tggcggatga
     2821 ggcaaaccgc aaatttttgc tggcactgac tttattttgc ggctcggcag taatctgcgt
     2881 ggcttcgatc tccaatgaca acctgcaaga cctgaaaacc ggctacctgc tcaaagccac
     2941 gccttggcgg cagcaagtcg ccctgattat cggctgtatc gtcggtgcgc tggttatttc
     3001 acccgtgttg gaactgcttt acgaagccta cggctttacc ggcgcaatgc cgcgcgaagg
     3061 tatggacgcg gcgcaggctt tggcagcccc tcaagcgact ttgatgacga ccatcgcgtc
     3121 gggcattttc gcccacaacc ttgaatgggt ctatatcttt accggcatcg cgattggcgc
     3181 ggtgttgatt gccgccgatt tggtgttgaa aaaatcatca ggcggcaaac ttgccctgcc
     3241 cgtccttgcg gtcggtatgg gtatttatct gccgccgtcc gtcaatatgc ccatcgtggc
     3301 aggcgcggtg ttggcggcgg tgttgaaaca catcatcggt aaaaaagcgg aaaaccgcga
     3361 aggccgtctg aaaaacgccg agcgcatcgg aaccttgttc tccgccggcc tgattgtcgg
     3421 tgaaagcctg atcggtgtga ttatggcgtt tattattgcc ttctccgtga ccaacggcgg
     3481 ctcggatgcg ccgcttgcgt tgaatctgca aaactgggat gccgccgctt cttggttggg
     3541 tttggcgttc ttcgttaccg ggatgttttt ctttgcacag cgcgtactga aggcgggtag
     3601 gtagcctgtc ggaaaaaatg ccgtctgaaa cgttcagacg gcatttttta tcgggaaagc
     3661 gtaaggcgga gcttttcggc ttgcgcccac gctttaccgg caagatcttt
//
LOCUS       DAXHKX010000184         3622 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHKX010000184 DAXHKX010000000
VERSION     DAXHKX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.184"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(17..250)
                     /locus_tag="ACFMWZ_001901"
     CDS             complement(17..250)
                     /locus_tag="ACFMWZ_001901"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935804.1"
                     /translation="MTAESHLLEQRSRLESLKREQNNAIRELDEQKITLSSLPERHKT
                     ELSQLNRAITEMNQEILDFDLKSEQTIRASKSG"
     gene            complement(376..825)
                     /locus_tag="ACFMWZ_001902"
     CDS             complement(376..825)
                     /locus_tag="ACFMWZ_001902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935805.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKMFITTFIV"
     gene            complement(839..1009)
                     /locus_tag="ACFMWZ_001903"
     CDS             complement(839..1009)
                     /locus_tag="ACFMWZ_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935806.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     ILIIGIFLSIFF"
     gene            complement(2050..2286)
                     /locus_tag="ACFMWZ_001904"
     CDS             complement(2050..2286)
                     /locus_tag="ACFMWZ_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC5935807.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            complement(3320..3622)
                     /locus_tag="ACFMWZ_001905"
                     /pseudo
     CDS             complement(3320..3622)
                     /locus_tag="ACFMWZ_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1132 a          661 c          622 g         1207 t
ORIGIN      
        1 tatttttgca aaggtctcaa cctgatttac tagctcgtat ggtttgttcg gatttcaaat
       61 caaaatccaa aatttcttgg ttcatttccg taatcgcacg gttgagttgg ctcaattcgg
      121 ttttatggcg ttcaggcagg ctgcttaatg tgattttctg ttcatcaagt tccctgattg
      181 cattattttg ttcacgtttt aggctctcca aacgtgagcg ttgttccaat aaatggcttt
      241 cggcggtcat cttatcttgt tgggatactg cgccttgact ggctaaaaac ttgttcttgt
      301 taagggtttt ttccgctaaa cgaatttgac gattttgccc tgtaatttgc tgtttaatat
      361 tctctaattg attgtttaaa cgatgaatgt tgttatgaac attttttgct cattttgatg
      421 aatgcgcttt aaacgttcca attcttgtaa tgccaaagtt tttttaaggt tggcttctgc
      481 tgccaatttg gcttgtacgt ttcctttttc gccaaaacgc gatgtggaaa gtttgaacaa
      541 tggttcgcca gctttgacaa agttaccatc ttcaacaaat ttatgcgtaa tcgtgccgat
      601 atcggaagag taaacacgaa ccacccccat agttggaagt aattgacctt caacggttgt
      661 tttattggta tagctaccaa aaatcaaaaa gataatgata cacagagcaa tgagaaaagc
      721 gcaaaaagtc agaaataaaa aagagaatgg acgggtcaag ataacctgac ctgtccactt
      781 attttgttgg gctacaaaga cttcttttcg gaataagtgg gacatttatt attatttctt
      841 aaaaaaaaat ggatagaaaa ataccaataa ttaaaatact tatgcctaat aacataaatt
      901 tgctattgat tgatgtttct attttaatta cattaaattt aaccaataat ccagaaatgg
      961 ttgcagctaa aatgcaaaag acagtggcgt aaatggtttc agtattcata atttttctct
     1021 ttcaaaattg ttcaatctaa atagaaaaag gtaacttcaa tacatattgc tgtaactgaa
     1081 attacctttt ccttaatgat gtaattatct tatggcttca tcatgccacg accgatattg
     1141 tatccgccag cagcagagaa aataccagct ccaattgcgc caacccagcc tactggtcct
     1201 gtccacttgt tccgttgagc tacaaagact tctggtctga aaaatgagtg attgttagac
     1261 atgtgtactt atttatgttt ctttgattgg gataaatttg taaaaacaaa aaaaaggcaa
     1321 gttgttatta tagaagaact taaaatgctt aacatactca ttgaggtttg taccacataa
     1381 tgagatacga gataatttgt tgggagtaaa atcaaaaaaa agattaaata tttaaaataa
     1441 tcactatatt ttttcattat ttgttccttt ttgtatttaa gttcttatga aaaactaggg
     1501 tattttgaat agaaaatatc aaaataccct agttttatta aggaatatta ccaccaacaa
     1561 gtgcttccat attgtacagc accagtgatt gcaccaccaa tagctccgaa tttcgcacct
     1621 ggaatagcac caataccacc tgcaaatgaa ccaacaattg ccccaccagc tgctccacct
     1681 aatgcactac caatggtatt ttttgagaaa tcacgccagt tacaagcagc accagaaact
     1741 tgttaaattt cattcaaagt caaaacttgc ataatattca cctttgttaa gtgtggttga
     1801 gaaaaaagta aattttcatc gttactcatt atttgcaatc atgcatatac tgtaaccaag
     1861 cactatattc agttggatca tagtttttcc tactgggttg tctgatatga ctattggcac
     1921 tattggcaca attgggatta gcaactttat tgtgagtacc taatggattg atatttcctg
     1981 taaagtggct attgcatgcg cttaatgagc atagatttat taatgtcaga aaggataaaa
     2041 caaaaaatct taccattttt tactaatacc aaaagtaggt ttcgaaaact gtacaccaag
     2101 accttgtaca tatccaaatt gcttgctaac agaaaactct actccactat tgttagggga
     2161 ataggaagca ccaattttac caacagcaac catatctttt ttacctaggt tgccgccacc
     2221 ctgtgcaaag atatggaatt tgccacctga cacttcaact aattcagagg tgtgtaactc
     2281 tttcatgatt ttctcctgta aagaagtatg gttggaaatg taaatcagaa ttaacctttc
     2341 ttaagtttaa tttaatactt tttttgcaag tattttattt taaattaaat caatatttta
     2401 aaaatattta cttgtatact gaaaaagaaa ctcctaatgg tggagtgggg cttttgttta
     2461 agtaagcatt aaaaaaattt agatttccca ttatgattaa cttaatgcaa gagctttttc
     2521 aggacgtaca aaccagcgaa cgggcgttct gatgtgggca ggttggttgt cgctaaccaa
     2581 tttcagtagc tcaatcgtca gattggtatc cgaccctatc aagctaagga gaggttgcgg
     2641 ctgctacaca aagcacctga aattattggg tgcttttgtc ttttatggca ttgtaccaat
     2701 ttgtgtacca cgagggcttg ttcgcccaaa cgcggtaaca actgaataca cactttcccc
     2761 ctcaacttct ttgcgttcta aaacgactgt cccaatccga gaatagacaa tcataggacg
     2821 atgattgccc cttacatcac taaattcact aaaaattttt gcgcctgatt gaaaaatcat
     2881 ttccacataa aagggttaga cctgtttcat cgtggcttaa tccaaaccgc gttaaatctg
     2941 ctttgtgttc tgccaaaatg tgaatcacac caaagccgtg attaaccttt ttgtgttcgc
     3001 cgtgttttaa aacaatttga cctgccggga agtctgctct attgttcgga caataggcgg
     3061 aatataccaa ttactttaac tacatcattt tttgacattt ttcttgcctt tcaatttaaa
     3121 atccaaaatc atactgccat aatttagcaa tccaaaaaaa tttaggcagc agtcatggtt
     3181 tcgtatttta gaaaacgaaa cttggtttgg tgttttgaag catttcagta cacaaacatg
     3241 gaattttaaa ttgtcttgtg ttgccttgat ttaggatgtt ccagtttttt aataaatagt
     3301 ttggggctgt cctagataac taggataaac tcgattttac taattgtttt aaaatggaac
     3361 aagaactttt atctcactgt tgttaaaacg ccattcgcac tcctttaaat acagctcaaa
     3421 atgcgctttg ggaatgccgt taaacttgcg taaatgacgt tttgcctggt tccaaaagtt
     3481 ctcaattcca ttaatatggt tttgtcgttc ggcaaaatgt gtgctgtgat tgatacgaaa
     3541 acgaagtttc agcgaagcta aaatggctaa attcgctcac atctaataca tcatagctac
     3601 gataacaatc cgtataaaca at
//
LOCUS       DAXHKX010000185         3602 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHKX010000185 DAXHKX010000000
VERSION     DAXHKX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.185"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            1..748
                     /locus_tag="ACFMWZ_001906"
                     /pseudo
     CDS             1..748
                     /locus_tag="ACFMWZ_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            745..1305
                     /locus_tag="ACFMWZ_001907"
     CDS             745..1305
                     /locus_tag="ACFMWZ_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFC5935808.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            1316..3289
                     /locus_tag="ACFMWZ_001908"
     CDS             1316..3289
                     /locus_tag="ACFMWZ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC5935809.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFPSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLRADGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTKTAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSSLVSTWTDENLSDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRDHTHIAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
BASE COUNT          883 a         1156 c          980 g          583 t
ORIGIN      
        1 tgcaagtgcg tatcggccag cgtttttacc gcactaccgc ccgcgccgtt atcggcagcg
       61 gcggcacggc ggaaataccg gcaatcgccg acgagccggg cgcggccgcc aatgtggcga
      121 cggcgaggcg caactgatgg ccgcccccgc cggtgtggcc accgaatgcc gccttaccgt
      181 acaaggcggc accgaccgag aaagcgatgc ctcactgctg gcgcgtctgt tgaaaatcat
      241 ccgccgaccg cccgcaggcg gcaaccgtta cgactataaa aactgggcgt tgagtgttga
      301 cggcgtaacc agcgcatatg tttatccgct gcgccgcggc ttgggtacgg tggatattgc
      361 cattacctcc gccgacggtg tgccatcgga agaaactgtg cgccgcgtac aggcttatat
      421 cgacgagatg cgcccggtaa cggcaaaaaa tgcgctggta ctcaagccaa ccgtaacggc
      481 ggtgcctgtt accgtgcaag tcaagctcga cggcatcgac ttggacgagg ccaagcgccg
      541 catacggacg gccctaaaag aatatttcga caccctgatc cccggcgacg gcctgactgt
      601 gtcgcaaatc gaggcggcta tcagcaatgt ggatggtgtg atcgaccgcc gtctgactgc
      661 gccgacggcc aaccgtgccg ccgatacggt taaccgcatc gagtggttta aagcgggcgc
      721 gattaatgta acggagatgc cgtcatgagc tatcaagaca tcttgcgggg cctgttgccc
      781 cccgtgtcgt atgcccgcaa tgccccgcgt gtgagggcgc aggcagaaat agacggcgca
      841 gcgctggatg cggtggcgga atcggctcaa agcgttgccg atgccgtcga cccgcgcagc
      901 gccggccaaa tgctggccga ttgggagcgc gtattaggtt tggacggtac gggcaaaaac
      961 cgccagcgcc gtgtgttggc cgtcatggcc aagctaaacg aaacaggcgg cttgagtatt
     1021 ccttattttg tgcgtttggc cgaggcggcg ggctatcaaa tccaaatcga cgaaccgcag
     1081 ccgttccgcg ccggtgtaaa ccgcgccggc gaccgtcttg cgccgcagga aatcatgtgg
     1141 gtgtggcacg ttaacgtgcg cggcggcaac aaccgcatta cccgattccg cgccggtatc
     1201 tcggcggcgg gcgacaggct gaccgattac agcgatgccg tgatcgagag cctgttcaac
     1261 cgcctcaagc ccgcccacac cgctatccga tttacctacc gataaggact aaaccatgca
     1321 ccccatcgaa acccccgaca agaccttcca cgacggcgac ggcgtgtccg aattgggcac
     1381 catcctgccc gcgtggtggc tcaaccaagt gcaatccgag ctgctggccg tgctgactgc
     1441 ggccggtatc cagccggata agtcgcagcc taatcaatta ctggcagcac tgaatagact
     1501 ggctgtagtt accaccggca accaagaaat cggcggccaa aaaaccttta ccgcccaaac
     1561 ccagttccca agcggcatcc atttatccgc caaccagacg aactggaacg gcggccacaa
     1621 agcctacatc ggcgcggatg ccgacaacgc ccacatcgtc ttcggcgaca acaccctccg
     1681 cctgcacggc gcaaacaacc gcatttccta caacaaccac gacatcttcc acaaagccaa
     1741 caaaccgcgt tttgccgaag acatcgaagg caaaccgaac acactgtccg gatacggcat
     1801 cggcaatttc aaagtcgaaa cattccgggg cgatttgaac accctcaaaa cagacggcat
     1861 ctattccctg ccgacggcgg gcggcagctc caacctgccc gttgaaaaca ccgcctgcca
     1921 tatccaagtc atcgccggca cgaaacacgg ctggtgcagg cagttgggtt atcccgccta
     1981 cacgtccgac gtgtacgaac gccaccaaac gagcagcgca aacgacaact ggtccgcatg
     2041 gaaaaaactc aattcagacg gcatccccgt cggcgcgatc gtatcctttc ccaaagccgt
     2101 ccgaaacccc gcaggctatt tgcgggcaga cggcacaacc tttgcgcaaa acaccttccc
     2161 cgacctttac cgagccttgg gcaacagcaa ccgcctgccc gatttaagcc gtaccgacat
     2221 cggcatcacc gcgtggtttc cgtccgacca aatcccgacc ggctggctgg cgtttgacga
     2281 catccgcacg cgcgtaacca aaaccgctta tcccgagctg taccgcctgc tgaccggaaa
     2341 atacggcagc atccaaaacg tcccgcaggc ggaagaccgc tttatccgca acgcaggcaa
     2401 cagcttggca gtcggaacga agcaggaaga cgaaatcaaa cggcacgtcc acaaagtatt
     2461 ttcacactgg gtaaaccacc cgcacgcggc agccctcggt tacgaagacc acaacgaaag
     2521 gcagagaagc tcgctcgtat cgacttggac ggacgaaaat ttaagcgaca acggcttttt
     2581 aaccccgcgc ccggacagca aaatggcaac gggcggcgac gaaaaccgcc ccaaagccct
     2641 ggttttaaaa ctgtgcatca aagccgccga caccttgggc gaagccgtgt tctggataaa
     2701 gtcccacggc gaaaccatca acgcaggcgc gctggacgcg ggcacgctgg cgcaaaacct
     2761 ccaagacaaa gccgaccgcg accacaccca catcgccgcc caaatccaag ggctggacga
     2821 aaaaatcagc accgccgttg ccgcgcaatt cacacgccaa accatcggcg gcgtggatat
     2881 tgtcagattc cccgacggca caatgataca gaccggcagt tacaggttta caagaagcgg
     2941 cggccccatc gaaaacgaag tcgtcttccc cgtcgccttt gccgacggca acgtcaaatg
     3001 cttcgtatcc gaacgccatt cgggacgcgt taccggcgat cgaaggcaac acaactggct
     3061 gtttatccgc gcaaaaaacc acgccgccgc cattattacc aactggtacg aaggcagttg
     3121 cgactggatg gccatcggca aagccgcctc gggaaacgcc gccagctccc cgataggccc
     3181 cgaaatacct gaaaccaacg aagaaccgca aagagagagt ggaagaacat caaccggacc
     3241 ccgaaaccgc cgccgccgag acggcttgct cgaggcactg caagactagc gggctgtaga
     3301 gatgggttgt agagatggtt gtagagatag ctgtagagat ggctgtagag atgggttgta
     3361 gagatgggtt gtagagacga gttgtagaga tgggcttcag cccgccgatc caagcaatcc
     3421 gaccgaaacc ggccgcgccg ccaatccccc gaaacctatg ccccgccaat cctgccactc
     3481 ttcgtcattc ccgccgcttt cgtcattccc gcgaaagcgg gaatccagcc cctaaacgcg
     3541 gcaggaatct atcggaaaaa acagaaaccc ccgccaccgt cattcccgcg aaagcgggaa
     3601 tc
//
LOCUS       DAXHKX010000186         3560 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHKX010000186 DAXHKX010000000
VERSION     DAXHKX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.186"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(122..976)
                     /gene="tsf"
                     /locus_tag="ACFMWZ_001909"
     CDS             complement(122..976)
                     /gene="tsf"
                     /locus_tag="ACFMWZ_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC5935810.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(1100..1828)
                     /gene="rpsB"
                     /locus_tag="ACFMWZ_001910"
     CDS             complement(1100..1828)
                     /gene="rpsB"
                     /locus_tag="ACFMWZ_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC5935811.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(2005..2238)
                     /locus_tag="ACFMWZ_001911"
     CDS             complement(2005..2238)
                     /locus_tag="ACFMWZ_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935812.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLFFWAVLPTVFTVFV
                     VTIRRRNRLMRQQEQAEFEQQRAQRQKDSGTKP"
     gene            2316..2897
                     /locus_tag="ACFMWZ_001912"
     CDS             2316..2897
                     /locus_tag="ACFMWZ_001912"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC5935813.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPVEAHDRSLDGFVSEAGILCF"
     gene            2958..3548
                     /locus_tag="ACFMWZ_001913"
     CDS             2958..3548
                     /locus_tag="ACFMWZ_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC5935814.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     QRTRMNTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMYMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT          785 a          819 c         1024 g          932 t
ORIGIN      
        1 tgttttgttt tcagacggcc tttatattcg gtcgtcttaa aataggtacc gcaaacggtt
       61 ttctcaaaaa aaagcaccct gattcgtttg gaatccaggt gctttctttt tcataagtgc
      121 cttacacttt ggcggctgcg gctacttcgg ctgcgtagtc gacaacggct ttctcgatac
      181 cgtcgcctac tttgtagcgg acgaagctga ccacttcagt gccgttttct ttggcgaatt
      241 gggcaacagt ttgatcgggg ttcatcacga atgcttggcc gttcagagtg atttcagcca
      301 agaatttgcg gatacggcct tcaaccattt tagcggcgat gtcggcaggt ttgccggaag
      361 cgatggcttg ttcagtgtag atgtggcgtt ctttttcaac ggtttcagca tctacttcgg
      421 cttcgcttac gcattgtggt ttagcggcaa caatgtgcat accgattttg cgtgctacgt
      481 cttcagagcc tttgtactca accaatacgc cttcggtagc caatgcgccg tggatgtagg
      541 caaccagttg gttggcagtg tcgatcactt ggaagcgacg gacagacatg ttctcgccca
      601 atttggcgat gatggctttg cgttctgctt caaccagttc gctcagttct tcaacagaag
      661 ccggtttttt ctcggcagca gttttagcaa cgaagttggc gaattctacg aagccggcat
      721 ctttagcaac gaagtcggtt tcgcagttta cttcaaccaa tgcaccgaca ttgccgttga
      781 tcgcgtaagc caatacgcct tcggcagcag tacggccggc cagtttaccg gctttcgcac
      841 cagatttaat acgcaggatt tcttcggctt tgtcgaagtt gccttcagct tcaaccaagg
      901 cttttttgca ttccatcatg cccaggccgg tagcggcgcg caggtcggca accatttttg
      961 cagtaatttc tgccattttg aatctcctag atttttggga acacggccat tgccgtgttt
     1021 ggaaatagtg gccgtctgaa acggaattca gaacgggatt tgagaaaagg ggcataacgc
     1081 ccctcttctg tgtaccgaat tactcggcag cagcttcttg ggcagcggct acagtttctt
     1141 gcagcgcttg gtttttgcct tccaaaactg cgtcagcgat gccgcggcag tacaggcgga
     1201 tggctttggc ggagtcatcg ttaccgggga taacgtattt cacgccgtca gggctgttgt
     1261 tggtatcgac tacggcgata acagggatgc ccaatttttc agcctcaacc agagtacctt
     1321 tttggtagcc ggtatcgata acgaaaatcg cgtcaggcag gcctttcatg tttttgatac
     1381 cgcccaaaga acgttccagt ttttcaacgt cgcgttgcat ttccagaatt tcttttttgt
     1441 tgaaaccgcc ttcggcagca ttttccaagg ctgcggtttt ttcttccagg cgtttgatgg
     1501 attgcttaac ggttttgtag ttagtcagca taccacccaa ccagcggtaa tcaacgaaag
     1561 gcataccggc acgggtcgct tcttcgcgga tgatgtcgcg ggcttggcgt ttggtaccta
     1621 cgaacaatac tgtacctttg ttggcaacca gacgacgtac ggcttcttgc gcctcttgga
     1681 acatcggcag ggttttttcc aagtttacga tatggatttt gttgcgcgcg ccgaaaatgt
     1741 attgtgccat tttcggattc cagaaacggg tttggtggcc gaagtggaca ccggcttcaa
     1801 tcatctgacg catagtaatt tgagacattt tgatttcctt gaaagggtta agggcacaca
     1861 ttcaatcgca tagaacttga taggcgatgc ctattgtcaa gcacccttcg gcgaaagtgg
     1921 gcataagttt taaaaaataa aagagtgttt ccgaaatgga aaactgacgg attatagcgg
     1981 ataagaggcc gtctgaaaag ggattcaggg ttttgtgccg ctgtcttttt gccgttgtgc
     2041 gcgctgctgt tcgaattcag cctgttcctg ctgcctcatc aggcggttgc ggcggcggat
     2101 ggtcacgacg aagaccgtaa acacggtggg caacactgcc caaaaaaata aataaatcaa
     2161 cgcgcgcgca atactcggct gcgcggcgga aaacatcacg gcaacaaaaa gatagccgat
     2221 ggcgacaatg tgccacattg tgtgcgtcct gtttgaatgt taataaaatc gttataattg
     2281 caacattcta ccgcaatccc gaaaggccgt ctgaaatgag gaacgaggaa aaacacgccc
     2341 tgcgccgcga attacgcggg cggcgttcgc aaatggggtg ggacgtgcgg gcggcggcaa
     2401 cggtaaaaat taaccgcctg ctcaaacgtt atatcaagca cggtcggaaa atcggcgtgt
     2461 attggccgat gggcaaggaa ttgcgtttgg acggctttgt ccgcgcggcg caaaaacgcg
     2521 gcgcaaaact ttatctgcct tatatcgaac cgcgttcgcg gcggatgtgg tttacgccgt
     2581 atcctgaaag cggaatggaa cgggagcgca tacggggcag ggcgaggttg aacgtgccgc
     2641 agtttgcagg gcgcaaaatc cgcgtgcacg gtttgtcggt attgctcgtt ccgcttgtcg
     2701 gcatagaccg cgaaggctac cgtttggggc aggcaggcgg ctattacgat gcgacgcttt
     2761 cagcgatgaa ataccgtttg caggcaaaaa ccgtgggcgt gggctttgcc tgccagttgg
     2821 tggacagact gccggtcgag gcgcacgacc ggtctttgga cggttttgtg tcggaggcgg
     2881 ggatattgtg tttttagccg ttcagacggc attccgggcg gaaatgccgt ctgaaggcgg
     2941 aatattggga gaaaaggatg tcggaaaaga taatactgcc ggtcttgtcg ttgggcggcg
     3001 ttcgtctcga accgttggat gtgcatcatg aaacgggttt gcgcgaggcg gtttgcgatg
     3061 gggaggtttg gaagctgggg gtaacgtccg cgccccatcc ggatagggtg gcagactata
     3121 tcgggacagc attggcaacg cgtttggcgt ttgcggttgt cgatgaagag gcgggcaggg
     3181 tggtcgggac aacggcgtat tatcattttg aaccgcagat tccgcgtttg gatattggat
     3241 tcacatggta tgcggcatcg gcacagagga cacggatgaa cacctgttgc aaaatcatgc
     3301 tgttggatta cgcatttgat gttttggctt gccgttgcgt gggatggcgc acggatattc
     3361 tgaaccttgc ctcgcaacgt gccatcgaac ggctgggtgc ggaaaaagac ggcgtgctgc
     3421 gtatgtatat gctccgtaaa gacggcagtg tgcgcgatac ggtcgtgtac agtatgctga
     3481 gggaagattg gtgcaaaaac agggaaatcc tgaccggcag gttggcaggg tatggcgtgc
     3541 aggtgtgagg cagtattttc
//
LOCUS       DAXHKX010000187         3492 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHKX010000187 DAXHKX010000000
VERSION     DAXHKX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.187"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..72)
                     /locus_tag="ACFMWZ_001914"
     CDS             complement(<1..72)
                     /locus_tag="ACFMWZ_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC5935815.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTA"
     gene            332..1804
                     /gene="pyk"
                     /locus_tag="ACFMWZ_001915"
     CDS             332..1804
                     /gene="pyk"
                     /locus_tag="ACFMWZ_001915"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC5935816.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRTGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            2015..3409
                     /locus_tag="ACFMWZ_001916"
     CDS             2015..3409
                     /locus_tag="ACFMWZ_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC5935817.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVFPSIRYEADSATDFTGIPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIPKKAQMLQATPSNPTAAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWNSNM
                     LAPLGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          909 a         1059 c          867 g          657 t
ORIGIN      
        1 tgcagttaca cctgcgacaa atagctcaat gagtttattt tgtttatacc ggcttagacg
       61 actttttctc atagggataa ttctaactta atttgaattt ccctagttat ctaggacagc
      121 cccaaaataa atttagccta agtcataaaa ccgtgtaatt ttgcgccaaa aaaggcggtt
      181 tgcaggtaaa atatcaaact attaatttaa atcatatttt tacagaatat tccgccgcgt
      241 tcatcaaatg gacatcaaac cggttccaaa tttctgtaat tttgtaacaa aataccgcaa
      301 aacacccgat tgagaccaaa aggactttca tatgaaccag acaagccgcg atctgacccg
      361 catcagccac aacactaaaa tcgtcgccac ccttgggccg ggcagcaaca acgtcgaact
      421 gttggaagac atgatccgcg tcggcggtct gaacgtcgtc cgcttcaact tcagccacgg
      481 cacgcccgaa ttccatcagg aaaacgcccg catcgtgcgc gaggcggcaa aacgcaccgg
      541 acaggaaatc gccatcattg ccgacctgca gggcccgaaa atccgcgtgg gcaaaatcgc
      601 cggcggcggc atcgaattga acaaaggcga aacgctggta ctggatgccg cgctcgaagg
      661 cgaaggcacg cgcgaggcgg tcggtttgga ctaccgcgac ctgcccgacg acgttgccgc
      721 aggcgatgtc ttgtggctgg acgacggcct gctgaccctg accgtggaat ccgtcgaagg
      781 cagcaggatt atcacaaggg tggaaaacag ccacgtcctg aaaagcaaca agggcatcaa
      841 caaacgcggc ggcggtctgt ccgcaggcgc gttgaccgaa aaagacttcc gcgacctgaa
      901 aaccgcgatt gccatcggtt gcgactacct cgccatcagc tttgtgaaat ctgccgaaga
      961 tttgcacatc gcgcgcgcca aagtcgaaga agaaatgaag ggcagcactg ccgtgcgccc
     1021 cggtttggtt tccaaaatcg aacgcgtaga agcgattgaa aaccttgatg aaatcatcct
     1081 cgccggcgac ggcattatgg ttgcacgcgg cgacttggcg gtcgaagtcg gacacgccgc
     1141 cgtccccgcc ctgcaaaaac ggatgatccg ccgcgcccgc gagttgcgcc gcttcagcat
     1201 tacggcgacg caaatgatgg aatcgatgat taccaacccc gtgccgaccc gcgcggaagt
     1261 cagcgatgtg gcaaacgcgg tattggacgg caccgatgcg gtgatgtgtt ccgccgaaac
     1321 cgccgtcggc gcgtatccgt ttgaaaccgt cagccaaatg gcgattatct gcgcggctgc
     1381 ggaaaaagag caggattcgc tcaacggcgt tgccgaacag gtcgagtatc ccgaagcggt
     1441 cagcaccaac ctggcggttg ccggcggtgc ggtcagcgtg gcgcgcgcgg ttcacgccaa
     1501 agccatcgtc gccctgaccg aaagcggttc gaccgccttt gaaatcagcc gccacaacat
     1561 caccctgccg attttcgcgc tgaccccgag cgtttccgcc caacgccgta tggcgatgta
     1621 ccggggcgtg cgcccgctga ttttggcaac cagtaccgac cacgacacgg cgttaaacga
     1681 agtcgaaacg atgctggtgg aacacaacat cctgcattcc ggcgaccaat acatcattac
     1741 cagcggttcg caaatgcgcg aatccggttc gaccaatacg ctggaagtgc tgcgcgtcaa
     1801 ataatccgcc ctgagtggaa aatgccgtct gaagccgatg cccgaggctt cagacggcat
     1861 tttttgcggc ggcgcggcgg ttcgggcaaa ctctgaaaaa attgtgcaaa tccggcaaca
     1921 tcggataaaa tcgagtacct atactaaagc gaaacaaggc atttccgact gcctttttta
     1981 tttatccacc gtccgccttt ttacggaaac cgaaatgacc ccttccgcac tgaaaaaaac
     2041 cgtcctgctg ctcggcactg cctttgccgc cgcatccgca caagcctccg gctaccactt
     2101 cggcacacag tcggtcaacg cgcaaagcac ggcgaatgcc gccgccgcag aagccgccga
     2161 cgcatcgacc atcttctaca accccgccgg cctgaccaaa ctcgacagca gccagatttc
     2221 cgtcaacgcc aatatcgtat ttccaagcat ccgttatgaa gcggattccg ccaccgactt
     2281 taccggcatt cccgtccaag gttcgaaaag cggcaaaatc accaaaacca cggttgcgcc
     2341 ccacatctac ggcgcataca aagtcaacga caatctgacc gtaggcttgg gcgtgtacgt
     2401 ccccttcggc tctgccaccg aatacgaaaa agattccgta ttgcgccaca acatcaacaa
     2461 actcggtctg accagcatcg ccgtcgaacc cgtcgccgcg tggaaactca acgaacgcca
     2521 ttccttcggc gcaggcatca tcgcccaaca tacttctgcc gagctgcgca aatatgccga
     2581 ctgggggatc ccaaaaaaag cgcaaatgct gcaagcaaca ccttctaatc ctactgccgc
     2641 tgctcaaatc aaagccgacg gacacgccga tgtcaaaggc agcgattggg gcttcggcta
     2701 ccaactggcg tggatgtggg acatcaacga ccgcgcacgc gtgggcgtga actaccgttc
     2761 caaagtctca cacacgctca aaggcgatgc cgaatgggcg gcagacgacg caatggcgaa
     2821 acaactgtgg aatagcaata tgctcgcacc gctcggctac acggcgaatg aaaaagcccg
     2881 cgttaaaatc gttacgcctg agtctttgtc cgtacacggt atgtacaaag tgtccgataa
     2941 agccgacctg ttcggcgacg taacttggac gcgccacagc cgcttcgata aggcggaact
     3001 ggtttttgaa aaagaaaaaa ccatcgtcaa cggcaaatcc gaccgcacca ccatcacccc
     3061 caactggcgc aacacctaca aagtcggctt cggcggttct tatcaaatca gcgaaccgct
     3121 gcaactgcgc gccggcatcg cttttgacaa atcgcccgtc cgcaacgccg actaccgcat
     3181 gaacagcctg cccgacggca accgcatctg gttctccgcc ggcatgaaat accatatcgg
     3241 caaaaaccac gtcgtcgatg ccgcctacac ccacatccac atcaacgaca ccagctaccg
     3301 cacggcgaag gcaagcggca acgatgtgga cagcaaaggc gcgtcttccg cacgtttcaa
     3361 aaaccacgcc gacatcatcg gcctgcaata cacctacaaa ttcaaataaa cggcccgccg
     3421 tttgattgta gaaatgccgt ctgaaacaga tttccgtttc agacggcatt gttttaggct
     3481 cgatttatag tg
//
LOCUS       DAXHKX010000188         3444 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHKX010000188 DAXHKX010000000
VERSION     DAXHKX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.188"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            36..704
                     /locus_tag="ACFMWZ_001917"
     CDS             36..704
                     /locus_tag="ACFMWZ_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC5935818.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNKCNSGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINDKRINYAFNHIAFGSSQPTADRVVWTFNQNGTFGYTK
                     DQHLTSKSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRNDRRTV
                     CQYK"
     gene            734..1345
                     /gene="pilV"
                     /locus_tag="ACFMWZ_001918"
     CDS             734..1345
                     /gene="pilV"
                     /locus_tag="ACFMWZ_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC5935819.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSVVDGDFQVGV
                     IKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTLSGNAFSSNCDNKAN
                     GDTLIKVLWVNDSAGDSDIARTNLEVSGDNIVYTYQARVGGRE"
     gene            1342..2280
                     /locus_tag="ACFMWZ_001919"
     CDS             1342..2280
                     /locus_tag="ACFMWZ_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC5935820.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVVSDVAQKNRL
                     FSLKRNSTNKLIPIAESLNIEYQGFAQARPALIFQYGIDDLDASAETVVVSSCSKIAK
                     PGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIADEEGLFRFQLNEKGK
                     WGNPQLLVKKVRHMKVRYIYVSGCPEDDDAGKEETFKYTDKFDSSTNAVTPAGVEVLL
                     SSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2259..2858
                     /locus_tag="ACFMWZ_001920"
     CDS             2259..2858
                     /locus_tag="ACFMWZ_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC5935821.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCEKGLCTAVNVRT
                     NTNNGNEEVFDNIVVQGKPTVEAVKRSCTANSTNLCIDNKGMEYKKGTRSVSKMPRYI
                     IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2863..3336
                     /locus_tag="ACFMWZ_001921"
     CDS             2863..3336
                     /locus_tag="ACFMWZ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC5935822.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAYSETLSADAGCEAFSNRKK"
BASE COUNT          994 a          682 c          872 g          896 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gtcctatgtg tacacgaaaa caacaaggtt
       61 tcacgctaac agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc
      121 tccccaatat gagccaatgg attgcatccc gccgcattgc cagtcacgcg gagcaggttg
      181 ccaacctttt gcgtttctcc aggggagaag ccgtccggct caatcttcct gtctatatct
      241 gtcctgttca agttaaaaaa ggcggtgcgc ccaacaataa atgtaactcc ggcaagaagg
      301 ggcagggaat gttggctttt ggcgataaga atggcaataa gacatatgac ggcgatacgg
      361 cggatgttct tctccgcagt gtggtattga atgatgatat caatgataag cggattaatt
      421 atgccttcaa ccatatcgct ttcggttcgt ctcagccgac cgccgaccgt gtagtttgga
      481 cattcaatca aaacgggacg ttcggttata cgaaagacca gcatcttaca agcaaatcca
      541 gcttttttta ttccgacggt tatatccaaa tcgtgctgac agatgcgaga gcagtttcag
      601 atgccgataa gaaattccgt tcggcggtgg ttttgattga cagcagcggc agggttgagg
      661 tttgtcctag aaacgatagg cgtaccgtat gccaatataa atgattgttt tagtcggttt
      721 gggaaataaa aaaatgaaga ataatgattg cttccgcctg aaaaaccccc agtccggtat
      781 ggcgctgata gaagtcttgg tcgctatgct cgttctgacc atcggtattt tggcactatt
      841 gtctgttcag ttgcggacag tcgcttccgt cagggaggca gagacgcaaa ccatcgtcag
      901 tcaaatcacg caaaacctga tggaaggaat gttgatgaat ccgaccattg attcggacag
      961 caacaagaaa aactataatc tttacatggg aaaccatcat acactatcag ttgtggatgg
     1021 cgattttcag gttggtgtca taaaaactaa gacgcagttg gcagaggcac aattgaagag
     1081 atttagttat gagctgaaaa atgccttgcc ggatgcggca gccatccatt acgccgtctg
     1141 caaggattcg tcgggtaacg cgccgacatt gtccggcaat gctttttctt caaattgcga
     1201 caataaggca aacggggata ctttaattaa agtattgtgg gtaaatgatt cggcagggga
     1261 ttcggatatt gcccgtacga atcttgaggt gagcggcgac aatatcgtat atacttatca
     1321 ggcaagggtc ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa aggcagttat
     1381 gatggtatga aaggttttac cattattgaa tttttggttg cgggcctgct cagtatgatt
     1441 gtcctgatgg cggtcggatc gagttacttc acatcccgga aattaaatga tgcggcaaac
     1501 gagcgtcttg ccgcacaaca ggatttgcgg aatgcggcaa cattgattgt ccgcgatgca
     1561 agaatggcag ggggcttcgg ttgtttcaat atgtccgagc atcctgcaac tgatgttgtt
     1621 tccgatgtgg cgcaaaaaaa ccgtcttttt tccttaaaaa ggaacagtac aaataaactt
     1681 atccccatag cggaatcttt aaatatcgaa tatcagggtt ttgcccaggc tcgtccggca
     1741 ttgattttcc aatacggaat cgatgatctt gatgcgagtg ctgagactgt tgtagtcagc
     1801 agctgttcca aaatagcaaa accgggtaag aaaatatcta ccttgcaaga agcaaagagt
     1861 gcattacaga ttactaatga tgataaacaa aatggaaata tcacccgtca aaggcatgtg
     1921 gtcaatgcct atgcggtcgg caggattgcc gatgaggaag gtttgttccg cttccaattg
     1981 aatgaaaagg gcaagtgggg taatcctcag ttgctcgtga aaaaggttag acatatgaaa
     2041 gtgcggtata tctatgtttc cggctgtcct gaagatgacg atgccggcaa agaggaaaca
     2101 ttcaaatata cggataaatt cgacagctcc acaaatgctg ttacgcccgc cggggtggag
     2161 gttttattga gtagcggtac tgataccaag attgccgctt cttcagacaa tcatatttat
     2221 gcttaccgta tcgatgcgac aatacgcggg ggaaatgtat gcgcaaacag aacactttga
     2281 cgggaatccc gacttctgac ggacagaggg ggtttgcact gtttatcgtg ctgatggtga
     2341 tgatcgtcgt ggcttttttg gttgtaactg ccgcgcagtc ttacaatacc gagcagcgga
     2401 tcagtgccaa cgaatcagac aggaaattgg ctttgtcttt ggccgaggcg gctttgcggg
     2461 aaggcgaact tcaggttttg gatttggaat atgatacgga cagtaaggtt acatttagcg
     2521 aaaactgtga aaaaggtctg tgtaccgcag tgaatgtgcg gacaaataca aataatggta
     2581 atgaagaggt ttttgacaat atcgtggtgc aaggcaagcc caccgttgag gcggtgaagc
     2641 gttcttgcac tgcaaattct accaacctgt gcattgacaa taaagggatg gaatataaga
     2701 aaggcacgag aagcgtcagc aaaatgccac gttatattat cgaatatttg ggcgtgaaga
     2761 acggagaaaa tgtttatcgg gttactgcca aggcttgggg taagaatgcc aataccgtgg
     2821 tcgtccttca atcttatgta agcaataatg atgagtaata aaatggaaca aaaagggttt
     2881 acattgattg agatgatgat agtcgtcgcg atactcggca tcatcagcgt cattgccata
     2941 ccttcttatc aaagttatat tgaaaaaggc tatcagtccc agctttatac ggagatggtc
     3001 ggtatcaaca atatttccaa acagtttatt ttgaaaaatc ccctggacga taatcagacc
     3061 atcaagagca aactggaaat atttgtctca ggctataaga tgaatccgaa aattgccgaa
     3121 aaatataatg tttcggtgca ttttgtcaat aaggaaaaac caagggcata cagcttggtc
     3181 ggcgttccaa agacggggac gggttatact ttgtcggtat ggatgaacag cgtgggcgac
     3241 ggatacaaat gccgtgatgc cgcttctgcc cgagcctatt cggagacttt gtccgcggat
     3301 gccggctgtg aagctttctc taatcgtaaa aaatagggct gttttgccaa tgccgtctga
     3361 aaatcaatgt tcagacggca tttttatggg tatagtggat taacaaaaat caggacaagg
     3421 cgacgaagct gcagacagta caga
//
LOCUS       DAXHKX010000189         3434 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHKX010000189 DAXHKX010000000
VERSION     DAXHKX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.189"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     regulatory      64..167
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            234..1457
                     /gene="cytX"
                     /locus_tag="ACFMWZ_001922"
     CDS             234..1457
                     /gene="cytX"
                     /locus_tag="ACFMWZ_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC5935823.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            1454..2554
                     /locus_tag="ACFMWZ_001923"
     CDS             1454..2554
                     /locus_tag="ACFMWZ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC5935824.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVVGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSLARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALVDGKDAPERDEESGLAYIRRQD"
     gene            2575..3192
                     /gene="thiE"
                     /locus_tag="ACFMWZ_001924"
     CDS             2575..3192
                     /gene="thiE"
                     /locus_tag="ACFMWZ_001924"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC5935825.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHGDE
                     LKREIARCAAACRGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          686 a         1068 c          961 g          719 t
ORIGIN      
        1 atgcgctgtg ccgccgaaaa tgtaagaatt ttgccaacgc acgttgcttt gtgtaaactt
       61 aaagctttct tgtcggagtg ccgccgccgg gcggctgaga ttgcgaaagc agaatccgta
      121 gaacctgtcg gggtaatgcc tgcgtaggaa acaaaccgtc aaatgcctta tcaggcttcc
      181 gttccctttt ccgcacttcc ccgcccacct tccttctttt taaaggacgt catatgtcgg
      241 gcaatgcctc ctctccttca tcttccgccg ccatcgggct gatttggttc ggcgcggcgg
      301 tatcgattgc cgaaatcagc acgggtacac tgcttgcgcc tttgggctgg cagcgcggtc
      361 tggcggctct gcttttgggt catgccgtcg gcggcgcgct gttttttgcg gcggcgtata
      421 tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg cctgtcgttc ggcaaacgcg
      481 gttcagtgct gttttccgtg gcgaatatgc tgcaactggc cggctggacg gcggtgatga
      541 tttacgccgg cgcaacggtc agctccgctt tgggcaaagt gttgtgggac ggcgaatctt
      601 ttgtctggtg ggcattggca aacggcgcgc tgattgtgct gtggctggtt ttcggcgcac
      661 gcaaaacagg cgggctgaaa accgtttcga tgctgctgat gctgttggcg gttctgtggc
      721 tgagtgccga agtcttttcc acggcaggca gcaccgccgc acaggtttca gacggcatga
      781 gcttcggaac ggcagtcgag ctgtccgccg tgatgccgct ttcctggctg ccgcttgccg
      841 ccgactacac gcgccacgcg cgccgcccgt ttgcggcaac cctgacggca acgctcgcct
      901 acacgctgac cggctgctgg atgtatgcct tgggtttggc agcggcgttg ttcaccggag
      961 aaaccgacgt ggcaaaaatc ctgctgggcg caggtttggg tgcggcaggc attttggcgg
     1021 tcgtcctctc caccgttacc acaacgtttc tcgatgccta ttccgccggc gcgagtgcga
     1081 acaatatttc cgcgcgtttt gcggaaacac ccgtcgctgt cggcgttacc ctgatcggca
     1141 cggtacttgc cgtcatgctg cccgttaccg aatatgaaaa cttcctgctg cttatcggct
     1201 cggtatttgc gccgatggcg gcggttttga ttgccgactt tttcgtcttg aaacggcgtg
     1261 aggagattga aggctttgac tttgccggac tggttctgtg gcttgcgggc ttcatcctct
     1321 accgcttcct gctctcgtcc ggctgggaaa gcagcatcgg tctgaccgcc cccgtaatgt
     1381 ctgccgttgc cattgccacc gtatcggtac gccttttctt taaaaaaacc caatctttac
     1441 aaaggaaccc gtcatgaccc gtatcgccat cctcggcggc ggcctctcgg gaaggctgac
     1501 cgcgttgcag cttgcagaac aaggttatca gattgcactt ttcgataaag gctgccgccg
     1561 gggcgaacac gccgccgcct atgttgccgc cgccatgctc gcgcctgcgg cggaagcggt
     1621 cgaagccacg cccgaagtgg tcaggctggg caggcagagc atcccgcttt ggcgcggcat
     1681 ccgatgccgt ctgaacacgc acacgatgat gcaggaaaac ggcagcctga ttgtgtggca
     1741 cgggcaggac aagccattat ccagcgagtt cgtccgccat ctcaaacgcg gcggcgtagc
     1801 ggatgacgaa atcgtccgtt ggcgcgccga cgacatcgcc gaacgcgaac cgcaactcgg
     1861 cggacgtttt tcagacggca tctacctgcc gaccgaaggc cagctcgacg ggcggcaaat
     1921 attgtctgca cttgccgacg ctttggacga actgaacgtc ccttgccatt gggaacacga
     1981 atgtgccccc gaagacctgc aagcccaata cgactggctg atcgactgcc gcggctacgg
     2041 cgcaaaaacc gcgtggaatg cttctgccga acacggcagc accctgcgcg gcatacgcgg
     2101 cgaagtggcg cgggtttaca cacccgaaat cacgctcaac cgccccgtgc gtctgctcca
     2161 tccgcgttat ccgctctaca tcgccccgaa agaaaaccac gtcttcgtcg tcggcgcgac
     2221 ccaaatcgaa agcgaaagcc aagcccctgc cagcgtgcgt tccgggctgg aactcttatc
     2281 cgcactctat gccgtccatc ctgccttcgg cgaagccgac atcctcgaaa tcgctaccgg
     2341 cctgcgcccc acgctcaacc accacaaccc cgaaatccgt tacagcctcg cccgacgcct
     2401 gattgaaatc aacggccttt tccgccacgg cttcatgatt tcccccgccg taaccgccgc
     2461 cgccgtcaga ttggcagtgg cactggttga cggaaaagac gcgcccgaac gtgatgaaga
     2521 aagcggtttg gcgtatatcc gaagacaaga ttaaagccgt ctgaaaggaa ccccatgacc
     2581 ttcccgccct tgaaatcccc gctcaaattc tacgccgtcg tccccaccgc cgattgggtc
     2641 gagcgcatgg tcgaagcagg tgccgacacg gtgcaactgc gctgcaaggc cctgcacggc
     2701 gatgaattga aacgcgaaat cgcccgctgc gccgcagcct gccgtggcag ccgtacgcag
     2761 cttttcatca acgaccactg gcgcgaagcg atagaagcag gcgcgtacgg cgtgcatctc
     2821 ggacaagaag acatggacac cgccgacctc gccgccattg ccgctgccgg tttgcgcttg
     2881 ggtttgagta cgcactccgt cgccgaactc gaccgcgccc tgtccgtgca ccccagctac
     2941 gtcgccagcg gcgcgatttt cccgaccacg accaaacaaa tgcccaccgc ccctcaaggc
     3001 ttggacaaac tgtgcgagta tgtgaaacag gcaggcggca cgcccgtcgt cgccatcggc
     3061 ggtatcgact tgaacaacgc ccgagccgta ctcgccaccg gcgtttcctc actcgccgcc
     3121 gtccgcgccg taaccgaagc ggcaaatccc gaagcggtgg ttaaagcgtt tcaggctttg
     3181 tgggatggat aaaaccgaaa gaagaaaatt caatttgccg tgtcggcaaa acttagcccg
     3241 ttatcgcaaa catacttaac tttaaatgtg gcatatcatc aaattccgtc atttccgcgc
     3301 aagcgggaat ccgccttaaa acttgagaaa ccatcatttg aaaaacagtt tccgaatttc
     3361 aaaaatggat tcccgcctgc gcgggaatga cggcaaccgg ccggttgcgt atcaaaaaat
     3421 aaagtaattc ggct
//
LOCUS       DAXHKX010000190         3401 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHKX010000190 DAXHKX010000000
VERSION     DAXHKX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.190"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(199..579)
                     /locus_tag="ACFMWZ_001925"
     CDS             complement(199..579)
                     /locus_tag="ACFMWZ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC5935826.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMSSVQNQARPADF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1056..3137
                     /locus_tag="ACFMWZ_001926"
     CDS             1056..3137
                     /locus_tag="ACFMWZ_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC5935827.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3127..3321
                     /locus_tag="ACFMWZ_001927"
     CDS             3127..3321
                     /locus_tag="ACFMWZ_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC5935828.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          757 a         1001 c          862 g          781 t
ORIGIN      
        1 tcattggata acagccctga agaaatcgga gcaggtttaa agttggcatt gagccgctgc
       61 cgataggata aacccggaac gaaacgaaaa ataatccgcc acgcccgaaa accgccgaaa
      121 ccagccgaca gccaaaacaa atccgacaca aaaaaccgcc cgaaaaatcc ggacggcggt
      181 tcaaacaggc tgccccgttt aacgggcgcg gcaggaagtt tcgaccgaat tgccgtaggc
      241 atcggtaaag ccgaaaaagg cttcgccgcc tttctggtgc cactcggttg cgtttccgaa
      301 caaaccgtgt tcggcggtat agcgttcgcc ggatgcggca acgtcggaag agaggacggc
      361 acgcctgccg tccagccgca acgcgacttt gccgctgtcc aaatggcgga cgcgcacaga
      421 caaaccgttc tcgcatgaaa acgcccgaaa atctgccggg cgggcttggt tttgaacgga
      481 cgacatatgc cctcgtccgc cgtcatcata cgcctccggc acggcacagg ccgccaaaga
      541 caaaaccggt acggtcagcg cgaaaaacct gatattcata aaagctcccc aataaaaata
      601 agatatgaaa caaccgccct gattccaagc tgcggcaacg ccatactata aacggacgcg
      661 caaacacaca agcccgataa ccggaattta cccgcgatga atcaataatc cggattgcgc
      721 gccccttctt taccggtctt ccggcgccgc cttttgcctg acgatgccgt ccaaacccgc
      781 ctgtccgccc gaaggaatgt aaattttttc caaattccaa gtaaaaaccg ctatcagtgt
      841 gctaatttgc gttaaaatcc tattcggcgt ttaacgtttt gtgcgcccgc atccctgcac
      901 tgtttgatgc gggcataagg cacaaatccc gacaagcgca ctgtttcata cttcgtcaat
      961 cattcagact ccggtttgtg cccgtgccgg cagatggttc ggccgtttcc cgccgttcag
     1021 gcatattccg acagtgtgag ataaggattt attcgatgaa atcactcaaa accttcctca
     1081 tttggggcat agtggtactg gtcggcttag catcctttac cactctggct ctcagccgag
     1141 gcgaacaggt cagcgcggta tggatggtta ccgccgccat atccgtttac tgcatcgcct
     1201 accgttttta cagcctctac atcgccaacc gcgtaatgcg gctcgatcct aaccgcctga
     1261 ctccggcaga acgccacaac gacggcttgg actacgttcc gacgcacaaa ggcgtattgt
     1321 tcggacacca ctttgccgcg attgcgggcg cgggcccttt ggtcggcccg gttttggcgg
     1381 cgcaaatggg ttacctgccc ggtactttgt ggattatctt cggcgtggtg tttgccggcg
     1441 cggtacagga tatgatggtc ttgttcgtct ctatgcgccg cgacggtaag tctttgggcg
     1501 atattgtgaa acaggaactc ggcactgtcc ccggcgtgat tgcctccatc ggtattttga
     1561 tgattatggt catcattatg gcggtgttgg cgttgattgt cgtaaaagca ctggttcaca
     1621 gcccttgggg tacgtttacc atcgccgcaa cgatgccgat tgccctgttt atgggtattt
     1681 acacgcgcta tatccgtccg ggcaaaatcg gcgagatttc catcgtcggc tttattttgc
     1741 tgatgctggc ggtaatttac ggcgaagatg tggcgaaaag ttccatcgga cattggttcg
     1801 accttgacgg catccagctc acttgggcga ttatgattta cggctttgtc gcctccgtat
     1861 tgcccgtatg gctgctgctc actccgcgcg actatctctc caccttcctg aaaatcggta
     1921 cgattgcggc cttggctttg ggcatcgtca tcgtcaatcc cactttgcaa atgcctgccg
     1981 taacccactt tatcgacggt tccggcccgg tattctcagg cgcattgttc ccgttcctct
     2041 tcatcaccat cgcctgcggt gcggtttcgg gcttccacgc gctgatttcc tccggcacca
     2101 cgccgaaaat gctggaaaac gaaacccacg tccgtatgat tggttacggc ggtatgttga
     2161 tggaaagttt cgtagccatt atggcacttg ccgccgccgc atcgcttgat cccggcgtgt
     2221 acttcgccat gaacagccct gccgccctga tcggtacgga tgccaatacc gccgccgaag
     2281 tgattaccac caagctgcaa ttccctgtcg atgctgcaac cctgttgcac accgctaagg
     2341 aagtgggcga aaacaccatc ctttcccgtg caggcggtgc gcccaccctc gcagtcggta
     2401 tggcgcacat tatgagccgc ctgattccgg gcgaggcgat gatggcgttc tggtatcact
     2461 tcgccctatt gtttgaagcc ttgttcatcc tgaccgccgt cgatgccggt acgcgcgtcg
     2521 cacgttttat gattcaagac ttgggcagca tcttctacaa acctttcggc aacaccgact
     2581 ccatccccgc caacctgatt gcgaccttct tcgccgtggc attgtggggc tactttctct
     2641 acaccggcgt aaccgacccg ctgggcggca tcaactcgct ctggcctttg ttcggcatcg
     2701 ccaaccaaat gctggcaggc gtagccttga ttatgtgcgc cgtggtgctg attaagatga
     2761 aacgcgaccg ttatgtctgg gtggtactcg ttcccgccgt cggcgtactg ttcgtaacct
     2821 gctacgccgg cctgcaaaaa ctgttccaca gcgacccgcg catcagcttc cttgcccacg
     2881 ccggcaaata cagcgacgca ttggctaaaa acgaagtcct tgcgcctgcc aaagacatcg
     2941 gcgaaatggc gcaaatcatc ttcaacgaca agattaatgc cggtctgacc atcctcttct
     3001 tgtcggttgt cgtgattgtc gccgcgtacg gtttgcgtac cgccctcaaa gcacgcaaag
     3061 tcggctggcc gaccgccaaa gaaatcccgg cggtgtaccg cgacggcaaa cagccggagg
     3121 cacaaagtga agcataagct cgcgtcttgg tggaaaacca tcaagctgac ggcaaacttg
     3181 atggcaggcg tgcccgatta tgaaaactac gttgcacagc agcgcaaaca taatcccaac
     3241 gcccctgtga tgaccaagct gcagtttcaa gactattgcc gcaaacgccg ctgcggcgca
     3301 aacggcggac gctgctgtta agcctgcttg aaacaaattc cgtctgaacg ccgcttcaga
     3361 cggaattttt ataatatagt ggattaacaa aaatcaggac a
//
LOCUS       DAXHKX010000191         3364 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHKX010000191 DAXHKX010000000
VERSION     DAXHKX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.191"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            187..1062
                     /gene="dapA"
                     /locus_tag="ACFMWZ_001928"
     CDS             187..1062
                     /gene="dapA"
                     /locus_tag="ACFMWZ_001928"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC5935829.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1071..2267
                     /gene="bamC"
                     /locus_tag="ACFMWZ_001929"
     CDS             1071..2267
                     /gene="bamC"
                     /locus_tag="ACFMWZ_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC5935830.1"
                     /translation="MPSEPFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKTTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2324..3256)
                     /gene="pip"
                     /locus_tag="ACFMWZ_001930"
     CDS             complement(2324..3256)
                     /gene="pip"
                     /locus_tag="ACFMWZ_001930"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC5935831.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEDVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT          890 a         1119 c          754 g          601 t
ORIGIN      
        1 ccgacggcac cggcataaac cggcgaatat aataaaatga tttcaaacag gaaaaatcaa
       61 tttccaccat acctttcggc gcaaacgccc cacatctgca acccgccgga tttcacacct
      121 gcggtaaagt aggctacaat cagaagcatt accgccccct acaccacaac tgataaaagg
      181 ttctgtatgt tacaaggtag cctggttgcc ctgattaccc cgatgaatca agacggcagc
      241 atccattacg aacaactccg cgatttgatc gactggcaca ttgaaaacgg cacggacggc
      301 atcgttgccg tcggcacgac aggcgaatcc gccaccctct ccgtcgaaga acacaccgcc
      361 gtcatcgaag ccgtcgtcaa acacgttgcc aaacgcgtcc ccgtcatcgc cggcacaggg
      421 gcaaacaaca ccgtcgaagc catcgccctt tcccaagccg ccgaaaaagc cggggcggac
      481 tacaccctct ccgtcgtccc ctattacaac aaaccttcgc aagaaggcat gtaccgccat
      541 ttcaaagccg ttgccgaagc cgccgcgatt ccgatgatac tctacaacgt acccggcaga
      601 accgtcgtca gcatgaacaa cgaaaccatc ctgcgccttg ccgaaatccc caacatcgtc
      661 ggcgtgaaag aagccagcgg caacatcggc agcaacatcg aactcatcaa ccgcgcgccc
      721 gaaggcttcg tcgtcctttc cggcgacgac cataccgccc tgccgttcat gctgtgcggc
      781 ggacacggcg tgattaccgt agccgccaac gccgcgccca agctctttgc cgatatgtgc
      841 cgcgccgcgc tgcaagtcga cattgccctc gcacgcgaac tcaacaaccg gctgattccg
      901 atttacgaca ccatgttctg cgaacccagc cccgccgcac cgaaatgggc ggtatccgcc
      961 ttgggcagat gcgaaccgca cgtccgcctg ccgctcgttc cgctgacgga aggcggacag
     1021 gcaaaagtcc gcgccgccct gaaagcctca ggacaacttt aaatatagcc atgccgtccg
     1081 aaccgttcag acggcataac gcaacaaaca ctttaatatc catcacacag gatgacacga
     1141 tgacccatat caaacccgtc attgccgcgc tcgcactcat cgggcttgcc gcctgctccg
     1201 gcagcaaaac cgaacagccc aagctcgact accaaagccg gtcgcaccgc ctgatcaaac
     1261 tcgaagtccc acctgatttg aacaaccccg accaaggcaa cctctaccgc ctgcctgccg
     1321 gttcgggcgc cgtccgcgcc agcgatttgg aaaaacgccg cacacccgcc gtccaacagc
     1381 ctgccgatgc cgaagtattg aaaagcgtca aaggtgtccg cctcgagcgc gacggcagcc
     1441 aacgctggct cgttgtcgac ggcaagtctc atgccgaaat ctggccgctc ctgaaagcct
     1501 tttggcagga aaacggcttc gacatcaaat ccgaagaacc cgccatcgga caaatggaaa
     1561 ccgagtgggc ggaaaaccgt gccaaaatcc cccaagacag cttgcgccgc ctattcgaca
     1621 cagtcggttt gggcggcatc tactccaccg gcgagcgcga caaattcatc gtccgtatcg
     1681 aacagggcaa aaacggcgtt tccgacatct tcttcgccca caaagccatg aaagaagtgt
     1741 acggcggcaa agacaaagac acgaccgtat ggcagccctc cccgtccgat cccaacctcg
     1801 aagccgcttt cctgacgcgc tttatgcaat atttgggcgt tgacggacag caggcggaaa
     1861 acgcatcggc aaaaaaaact acccttcccg ccgccaacga aatggcgcgt atcgaaggca
     1921 aaagcctgat tgtctttggc gactacggca gaaactggcg gcgcaccgcg ctcgccctcg
     1981 accgcatcgg gctgaccgtc gtcggtcaaa acaccgaacg ccacgctttc ctggttcaaa
     2041 aagccccgaa cgaaagcaat gcagttaccg aacaaaaacc cggcctgttc aaacgcctgc
     2101 tgggcaaagg caaagcggag aaacctgccg aacagccgga actgattgtc tatgccgagc
     2161 ctgtcgccaa cggctcgcgc atcgtcctgc tcaacaaaga cggcagcgca tatgccggca
     2221 aagacgcatc cgcattattg ggcaaactcc attccgaact gcgttaaacc attcaaaaca
     2281 aaagcagctt ccaaacagaa gctgcttttt tatgcggaac tttttacaac aaatggggca
     2341 aaatatcctc aactgcctga accaacgcat ccaccaaagg cggatcgaac gcacgatgcc
     2401 ctgcctgaac caccctcaat tccgcttcgg gaaaggcttt cgacagcgcc cacgcactct
     2461 gcatcggcgt acacaaatca taccgcccct gcacgataac ggtcgggata tgccgtattt
     2521 tgccgatatt gttcaaaatc gccttatcgc cctgcaacca gccgccgttg acaaaataat
     2581 ggttttccaa acgcgcaatc gccagtgagg cataagcatc ttcatccaca tcctccggct
     2641 cgaaacggat cagatagctt tcccaatccg cccaagcctt cgcggcagac aggcacactt
     2701 cttcatcttg atgaaacagc aacccgtgat acgcctcaat cagccgattc cgccgatttt
     2761 cagcaatcgg cgcgacaaat ttttgccatt gttccggata aatccggctc acaccgcccg
     2821 cctcgttcag ccacgccgtt tcagacggcc tgcacaaaaa tatcccgcgc aataccaatc
     2881 cctttacccg ttcaggatgg gtttgggcat aagccagcga caaagtgctg ccccacgaac
     2941 cgccgaacac cagccatttc ctgataccca gcatttcacg gactttttca atatccgcca
     3001 ccaaatccca agtcgtattg tcttccgcac aagcatacgg gcgcgaacgt ccgcaaccgc
     3061 gctggtcgat gatgacgatg cggaacacat cgggattgaa aaaaccccga cattcaggcg
     3121 aagcccccgc gcccggcccg ccatgtaaaa aaataaccgg cacaccgtcg ggattgcccg
     3181 attcctccca ataaatttga tgaatttcag acacctgcaa gtatccgcta tgaaaaggct
     3241 gttttatctc atacatcatc ttctcccata ttgcttctga caatccgaac attcccacac
     3301 gcaacatcac gcacgagaca caagccgccc cgaaaggcaa atgccgaaaa atcgcgatat
     3361 agtg
//
LOCUS       DAXHKX010000192         3362 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHKX010000192 DAXHKX010000000
VERSION     DAXHKX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.192"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            27..1196
                     /locus_tag="ACFMWZ_001931"
     CDS             27..1196
                     /locus_tag="ACFMWZ_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC5935832.1"
                     /translation="MNKKRVLLIASYDSFLNTGYAVAKEINYSTIDIYIHQTKKNILS
                     ESQLSLNGLSLKEINFFHIDDYFDKLMYQNYDIVILSLGNGLLTRFFRNSSKLNLTLP
                     IVITLFPGVIFGDSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPIL
                     RKKHHCSDGNKVYFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDIT
                     VHEDKYSYAELAKNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGV
                     ISDLGVDKKYANTHFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLL
                     EVTSTQPPSFDNYFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1244..2107
                     /gene="galU"
                     /locus_tag="ACFMWZ_001932"
     CDS             1244..2107
                     /gene="galU"
                     /locus_tag="ACFMWZ_001932"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC5935833.1"
                     /translation="MQKIRKAVFPVAGMGARFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEATVAYGLKHPELK
                     DDFKELLKQFV"
     gene            2190..3206
                     /gene="galE"
                     /locus_tag="ACFMWZ_001933"
     CDS             2190..3206
                     /gene="galE"
                     /locus_tag="ACFMWZ_001933"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC5935834.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPYEVKPRRAGDLACFYADPAYTKTQTGWETKRDLAQMMEDSWRWVSNNPN
                     GYDD"
BASE COUNT          997 a          631 c          719 g         1015 t
ORIGIN      
        1 acttaatatc taatatttaa aatacaatga ataaaaaacg agtcctatta attgctagtt
       61 atgattcttt tttgaatact ggatatgctg ttgcaaaaga aattaactat tcaacaatcg
      121 atatttatat ccaccaaaca aaaaaaaata ttctttcgga atcacaatta agtttaaatg
      181 ggctttcttt aaaagaaatc aatttttttc atattgatga ttatttcgac aaattaatgt
      241 accagaatta tgatattgtt attctatcat taggtaatgg tttactgacg cgtttttttc
      301 ggaatagtag caaacttaat ttaacattac ctatagtaat tactctattt ccaggggtaa
      361 tttttggtga ttcagaaagt attataacta ggttaggtgc taatatttta ttgttcaata
      421 attattatga ttaccaaata ggcaataagt tcaaagaacg ttacaactta tcgtgtacaa
      481 atattttata tggttatcct atcctacgaa aaaaacacca ttgctctgat ggcaataaag
      541 tttatttcat tgatcaagta aaaattccag atggaaaaaa agaaagatta ttaattctaa
      601 aaaaattaat agcactggct aatcgctatc cacataaaga tttagtacta ttattacgtg
      661 tccatcaaaa tgatatcaca gtacatgaag ataaatactc ttatgctgaa ttagcaaaaa
      721 atatgaaatt gcctgataat tttaaaatag agagaaaacg gacacaagat gccttacaag
      781 aaatgggata ttgtttatca ttctcttcaa caatgttatt tgaagctgaa agctgcggaa
      841 ttcctattgg tgtcattagt gatttagggg ttgacaagaa atatgccaat acacatttca
      901 aagatagtgg tgttttggtt aatttagata cggtagactt ggactctcca aataccatta
      961 atccaacttg ggctgaacta tatactaatc cagatagcct ctcttcaaaa gagtttaacc
     1021 aattattaga ggttacttct actcaaccac ctagttttga taattatttt attgcagtta
     1081 atgacgttca gcctgtatct atggttctgt tacgtaaatt taaaaagctt atatatcatc
     1141 ctaagcaatt ctttgttgat tcaaaactat ataaattaat taaatttact aaataatttt
     1201 tactatgggt tgccttattt atttcaaagg agtgacataa aaaatgcaaa aaattcgtaa
     1261 agctgtattc cctgtagctg gcatgggagc ccgattctta cctgccacga aagccagcgc
     1321 taaagaaatg ctgccaatcg tggacaaacc gttaattcaa tatgctgcgg aagaagccgt
     1381 tgccgcgggt tgtactgaat tgatttttat caccggccgt aataagcgta atattgaaga
     1441 ccacttcgat aaagcctatg agttggaaac tgaactggaa atgcgtgaaa agcataaatt
     1501 gcttggctat gtgcaaaaaa ttctgcctga tcatgtcagc tgtattttca ttcgtcaggc
     1561 agaagctttg ggcttgggtc atgcggtatt atgcgcccgt cctgctgtcg gtaatgagcc
     1621 ttttgccgtg attttggccg atgacttgat tgatgcacca caaggcgcac tcaagcagat
     1681 ggtagatatt tacaatcaaa gcggcaacag cattttaggg gtagaaacag tcgatccttc
     1741 gcaaaccggt tcatacggca ttgtcgaagt ggaacagctg aaaagctatc agcgtgtaac
     1801 caatattgtg gaaaaaccaa agcctgaaga tgcgccgtca aatttagcgg tagtcggccg
     1861 ttatattttg acaccgcgta tttttgattt gctgactaat ctacctcgtg gtgcaggtgg
     1921 tgaaatccag ctaactgatg gtattgcccg cctacttgac catgaatttg tcttggcgca
     1981 tgcgtttgaa ggtaagcgtt atgattgtgg cagtaagttg ggctatctgg aagccacggt
     2041 agcttatggc ttgaagcatc ccgaattaaa agatgatttc aaagaattat tgaagcaatt
     2101 tgtataattt taaggccgtc tgaaaaagaa aaggcatcat gttttttaca ctttcaggcg
     2161 gccttttaaa cataaagaag gaaaacgtga tgaacattct cgttaccggc ggcaccggtt
     2221 ttatcggctc gcacaccgtt gtttctttgc tgaaaagcgg tcatcaagtc gtgattttgg
     2281 ataacctatg caattccagc atcaatatcc tgccacgctt gaaaacgatt accggccaag
     2341 agattccgtt ttatcaaggc gatatccgcg atcgtgagat tttgcgccgt atttttgcgg
     2401 aaaaccgcat tgattcggtg attcattttg ccggcttgaa agcggtgggt gaaagtgtgg
     2461 ccgagccgat aaaatattat gataataatg tttccggcag cttggtgttg gcggaagaaa
     2521 tggcgcgtgc gggcgtgttt agcattgtgt tcagttcttc ggcgacggtt tatggcgatc
     2581 cgggcaaagt gccttatacc gaggatatgc caccgggcga caccaccagc ccttacggcg
     2641 catcgaaatc gatggttgag cgcattctca ctgacattca aaaagccgat ccgcgctgga
     2701 gcatgatttt gttgcgttat ttcaatccga ttggcgcgca tgaaagcggt ttgattggcg
     2761 agcagccaaa cggtatccca aataatttgt tgccttatat ctgccaagtg gcggcaggca
     2821 aactgccgca attagcggta ttcggtgatg actaccctac ccctgatggc acgggtatgc
     2881 gcgactatat tcatgtgatg gatttggcag aaggccatgt cgcggctatg caggcgaaaa
     2941 gcaatgtagc aggcacgcat ttgttgaact taggttccgg ccgcgcttct tcggtgttgg
     3001 aaatcatccg cgcatttgaa gcagcttcgg gtttgacgat tccgtatgaa gtcaaaccgc
     3061 gccgtgccgg tgatttggcg tgcttctatg ccgaccctgc ctacaccaaa acgcagaccg
     3121 gttgggaaac caagcgtgat ttggcgcaga tgatggaaga ctcatggcgt tgggtcagca
     3181 acaacccaaa tggctacgac gattaagttg acctgataca ggccgtctga aagagatgtt
     3241 ttcagacggc ctctttatct gaaaaacaca cattctgtct gctataatct gtttatattt
     3301 tttggctatc ctctgaaatt tatgagaaaa atcctcgtta ccggcggtgc gggctttatc
     3361 gg
//
LOCUS       DAXHKX010000193         3360 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHKX010000193 DAXHKX010000000
VERSION     DAXHKX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.193"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            103..1186
                     /locus_tag="ACFMWZ_001934"
                     /pseudo
     CDS             103..1186
                     /locus_tag="ACFMWZ_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743106.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
     gene            complement(1356..1565)
                     /locus_tag="ACFMWZ_001935"
     CDS             complement(1356..1565)
                     /locus_tag="ACFMWZ_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935835.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1562..1858)
                     /locus_tag="ACFMWZ_001936"
     CDS             complement(1562..1858)
                     /locus_tag="ACFMWZ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC5935836.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGLFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1837..2442)
                     /locus_tag="ACFMWZ_001937"
     CDS             complement(1837..2442)
                     /locus_tag="ACFMWZ_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935837.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATASRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAVIGKRQQLEDKLNAIETAPGLDALEKEIEEWTLNIG"
     gene            2522..2809
                     /locus_tag="ACFMWZ_001938"
     CDS             2522..2809
                     /locus_tag="ACFMWZ_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC5935838.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2781..3083
                     /locus_tag="ACFMWZ_001939"
     CDS             2781..3083
                     /locus_tag="ACFMWZ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC5935839.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
BASE COUNT          720 a          910 c          889 g          841 t
ORIGIN      
        1 aacacgcaat gtaccctgac cggcaaaact tccccaaact tcatcaaaaa atcaactgta
       61 cccgtctttt tcagacggca ttgataccgt aagaaatttc ccatgcagaa atggcaaacc
      121 gagctttatt ccacaccgct ttggctttgt ctgacaccga aaagcgcggt caaagtcttg
      181 ctgctgatta cggcgatgat tacgctggtg ttaaccgaaa tgcggctgaa tgtgttgagt
      241 acctttatgt ccaaagggct ttacgactcg atgcaggatt tgaaatgctt cggcattttg
      301 gatgtttgcg gcgatgaacg cgggcgttgt gctgatacgg gcgtttaaca acgtcgtcaa
      361 cgactttctc gatcaaggct tggcgattaa atggtcggaa cggctcaacg aagtgctgac
      421 agcacgctgg cttgccgaca aaaactacta ccgcctgcaa atgcgccgcc acgctccgga
      481 caacatcgac cagcgtatcc aacaggacgc gcaggaattt atcgcctcga ccatcgaatt
      541 tgtgcgtggt atggtcaatt cggtcgttac ttctctagaa tttgccgttg ttttgtgagg
      601 cttggcgggt atcctgaccg tgttcggctt cgacattccg cacggcattg tttggctcgt
      661 ctttattttt gtgattttgg cgacctttat cgcgatgtgg ataggcaacc ccttgattcg
      721 ttacaactat gaaaacgaaa aactcaacgg cgactaccgt tactccctca tccgcgtgcg
      781 cgaccacgcc gaaagcgtgg cgttttacaa cggcgaaaaa catgaatacg accagctttc
      841 cgaccgcttt aaagccatta tccgcaaccg ttggcgcatt gcgcggcaaa gtgtctgctt
      901 aagcggcttt aacgatatgt tcaccaacgg catcaaactc ttcccgatta ttttgcaagc
      961 tccgcgcctg tttgccggac aaatcaaaat cggcgacatc cagcaaaccg tccaagcctt
     1021 cgcgcgactg caaaacgcgc tgtctttctt ccgaatgttc tacaacaaat tcaccgccta
     1081 ccgcgcccga ttggagcgtc tgtacggctt tttgctgagt accgaagaac aacacagcgc
     1141 gcagcaaccc gagattagcg aagtttcaga cggcattgca ctggaatgta aggcggcggc
     1201 agcttttgca cgaattggcg gcagcttttg caccgccgaa gcgtaggaaa ttgtattagc
     1261 ttttgcacaa atttatcccc gtttaatggt ctgttaaacg gggattatct tatgccttta
     1321 aaagtagtac gctacttgcg gtcagaactt ataaatcagg tggtcgtcgt agcttgaatt
     1381 gctgttgatg tgctgctcac agtcaaggtt tggctgcctg caaaacgctc ccatccggtc
     1441 aggcgcagtt ctgccacgag gtcaagataa gcaggcagtt cgctgcgggt gccggagaag
     1501 aagacaaatg gcggccgcac cattgccatc aggcgcagaa actctaccat gccgaagtag
     1561 ttcatcgcgt ccccttttcc ccggcggcgc ggctttggtt gaaccgcgcg tcgtgctgcc
     1621 tgcctttcag ttcgctgata tacgcctgcc ggcagtgttg gcggtcgaaa aacaagccgt
     1681 taatcagggt gtataaaacc ttccagcggg ttttgggctt ttgggcgagc cgcgcgccgc
     1741 gataggtgcg gcttgagagg gtttcgtccg ccgcgccgcc ggttagggcg ttgaacagtt
     1801 ggtcgatggc taccaacagg tggtaaacgt attttttcag ccgatgttta gcgtccattc
     1861 ttcgatttcc ttttccagcg cgtccaatcc tggcgcggtt tcgatggcgt tcaatttgtc
     1921 ttcgagctgc tgccgctttc cgataaccgc gccggcggca acggccaggc gggcggattt
     1981 ttcgacaact ttttcaatca aaacgtccaa ttccacgccc cttgcggcgg cgatttgcgc
     2041 cagcatcggg gtcggggcgt tgttgtccgc ctgccgcgcg agggcttctt tttcctgcct
     2101 gtaaaagctg tcgatttcca cttggggata gcccgccaag aggctgtttt tgagttcgtc
     2161 cgcctttgcc gcgaggcggg atgccgtggc ggttttttgt tcggcgaaac gggcggcggc
     2221 ggcggcttcg ccgatttccc atttcttgcc gtcccattcg tggtattcgg acgggcgcgg
     2281 cggggttaaa acggggcggc cgtcggaatc tgcggcaatc tgcccgccct gcgcctgtcc
     2341 tgccaaaagg gcggcgtatt cttcggcgcg gacggcaacc gcgccttcgg ggatgccgcc
     2401 caaggtgtcg tcgtaaaagc cgtttttgaa atagatggtc atttcggggt ttcctttttt
     2461 gtatttacat tttggttttt tgccgcttat aatgtaacga caaaatacgc tgcaagctgc
     2521 aatgaagatt gaatttgact ccgaaaaaaa tcaacgcaat atcgaagagc ggaatttgcc
     2581 ctttgaatca gtcgggcaaa tacgctggac aacggccgtt attgtgcccg atgtccgctt
     2641 tgattacccc gaaccgcgtt atgttgccgc tgcttatcta ggcgatacgc aacgcctgca
     2701 cattgtctgt tttacgccga taaaagacgg catccgtgtc atcagttttc gcaaagcgaa
     2761 taaaagagag gttaaaaaat atgcaacgtc aatccttaac aaacgctgac ggcgaagtgc
     2821 gggaacttgc cgccgaagat tttgcgcttg cccgccccat tgccgaagcc ctgcccgaag
     2881 acttggcaca ggtgcttttt tcccatcaaa aacaattgga agaaaagggc gtaatgcaaa
     2941 aacgaaacac aggcaaaacc ccgaaacaac tcgttaccat acgcctgtcc gccgatgtgg
     3001 tcgaaaaatt ccgcgccggc ggcaagggct ggcagacgcg catcaacgag gttttgcgcc
     3061 aatatgtcgc ccaattgaaa taacaggggc ggccgctgct tatcgggcgc gcccgactat
     3121 cctttcccgc gcggtttcgg ccgtgcggtt ttttgttgcc ggtgccgtcc gaagggggca
     3181 gcggcttttt gcgcggtcat tcccgccttt cgttattccc gccactttcc gtcattcccg
     3241 cgaaggcggg aatcccgaaa cccctgccgc cgtcattccc gcgaaagcgg gaatccagtc
     3301 cgttcggttt cagccgtttc cgatagattc ctgccgcgtt tggggtctgg attcccgctt
//
LOCUS       DAXHKX010000194         3359 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXHKX010000194 DAXHKX010000000
VERSION     DAXHKX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.194"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..592)
                     /locus_tag="ACFMWZ_001940"
     CDS             complement(<1..592)
                     /locus_tag="ACFMWZ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC5935840.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADGEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPSADFLELLSLCRDIHALTDGAFDPTVQALWKLYADYFAARPDAETPPPQN
                     SVDEALKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQ"
     gene            complement(601..1395)
                     /locus_tag="ACFMWZ_001941"
     CDS             complement(601..1395)
                     /locus_tag="ACFMWZ_001941"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC5935841.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            1817..3151
                     /gene="gdhA"
                     /locus_tag="ACFMWZ_001942"
     CDS             1817..3151
                     /gene="gdhA"
                     /locus_tag="ACFMWZ_001942"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC5935842.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWNVAAEVALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT          886 a          945 c          805 g          723 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttcgtccacg ctgttttgag
      121 gaggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc agacggctaa
      301 tcagactgtc ttcacggtaa aggctgaaca ttttttccaa acgcgccact tccgccaaaa
      361 ccttattgac caaatccgcc gcctgtctgt cgtccacacc gaacaggcgc agctccgcgc
      421 cggaacccag cgcgacacct ttccagaaaa acacattttc attgcgtttt tcatccccgt
      481 ttcgtttttc accgtcggcg gcaaaaggat tcggcaggaa agaaaccgcc gcgcccgccg
      541 ccgcaacggc ggcaaccgtc agaaaacgcc tgcgcccgaa atgcctgccc ataccgcctc
      601 ctaaaccgac actgccgcct tgatgtgcgg atgcggatcg tagccttcca actcaaaatc
      661 ttcaaatttg aaggcaaaca aatctttgac ttcaggattg attttcatca caggcaaggc
      721 gcgcggttcg cgttccaact gcaatgcggc ctgctcgaaa tggttgcggt acaggtgcgc
      781 gtcgccaaac gtatggacaa actcgcccgc ctccaatccg cacacttgcg ccatcatcat
      841 ggtcaacagc gcgtagctgg caatattaaa cggcacacca aggaaaatat ccgcgctgcg
      901 ctggtaaagc tggcaggaca gtttgccgtc ggcaacgtaa aactgaaaca gcgcgtggca
      961 gggcggcaag gccatttcat cgaccaaagc cggattccac gccgatacaa tcaggcggcg
     1021 cgagtcggga ttcttcttga tttgttccag cacattggcg atttggtcga tatgcctgcc
     1081 gtcgggcgcg ggccagttac gccactggta gccgtaaacc gggcctaagt cgccgttttc
     1141 gtccgcccac tcgtcccaaa tggaaacatt gttgtccttt aggtatttga tattggtatc
     1201 gcctttgaga aaccaaagca gctcgtggat aatcgagcgc aaatgcagct ttttggtcgt
     1261 cagcagcgga aaacctttgc ccaagtcaaa acgcatctga taaccgaata cggagcgcgt
     1321 acccgtaccg gtgcggtctg atttgtccgt accgttgtcg aggacgtggc gcatcaagtc
     1381 caaataggcc ttcatcatca tctttcatca aattaaacgg cgcatattgt aacatttccg
     1441 gccaaagccc gagtccggac aaacgcacac gccgcccccc cggacgataa aacgcagata
     1501 cgggcaggca ggattgttgg caatttcagt ccttttccac agtaaaaccc agtgggaaaa
     1561 caaaattacc ttgattggaa tcaaaaaatc tagtttaatt acttagaata aaatttcaat
     1621 aatattttta tttacgaaat taatttatga ttattgatta attatcggca acaaacatca
     1681 aacatcgaaa atatggaaaa aataatgtca acaatttttg ccaaatcggg cttggcatca
     1741 gaaaaaaata ggtttatatt cccacctaca aatttgtttt cccattagta cactatcaac
     1801 caaaaggagt atccgaatga ctgacctgaa caccctgttt gccaacctca aacaacgcaa
     1861 tcccaatcag gagccgttcc atcaggcggt tgaagaagtc ttcatgagtc tcgatccgtt
     1921 tttggcaaaa aatccgaaat acacccagca aagcctgctg gaacgcatcg tcgaacccga
     1981 acgcgtcgtg atgttccgcg taacctggca ggacgataaa gggcaagtcc aagtcaaccg
     2041 gggctaccgc gtgcaaatga gttccgccat cggtccttac aaaggcggcc tgcgcttcca
     2101 tccgaccgtc gatttaggcg tattgaaatt cctcgctttt gaacaagtgt tcaaaaacgc
     2161 cttgaccacc ctgcctatgg gcggcggcaa aggcggttcc gacttcgacc ccaaaggcaa
     2221 atccgatgcc gaagtaatgc gcttctgcca agcctttatg accgaactct accgccacat
     2281 cggcgcggac accgatgttc cggccggcga catcggtgta ggcgggcgcg aaatcggcta
     2341 cctgttcgga caatacaaaa aaatccgcaa cgagttttct tccgtcctga ccggcaaagg
     2401 tttggaatgg ggcggcagcc tcatccgccc cgaagcaacc ggctacggct gcgtctattt
     2461 cgcccaagcg atgctgcaaa cccgcaacga tagttttgaa ggcaaacgcg tcctgatttc
     2521 cggttccggc aacgtggcgc aatacgccgc cgaaaaagcc atccaactgg gcgcgaaagt
     2581 gctgaccgtt tccgattcca acggcttcgt cctcttcccc gacagcggta tgaccgaagc
     2641 gcaactcgcc gcgcttatcg aactgaaaga agtccgccgc gaacgggttg ccacttatgc
     2701 caaagagcaa ggtttgcaat actttgaaaa ccaaaaaccg tggaacgttg ccgccgaagt
     2761 cgccctgccc tgcgcgaccc agaacgaatt ggacgaagaa gccgccaaaa ccctgttggc
     2821 aaacggctgc tacgtcgttg ccgaaggtgc gaatatgccg tcgactttgg gcgcggtcga
     2881 gcaatttatc aaagccggca tcctctacgc cccgggaaaa gcctccaatg ccggcggcgt
     2941 agcaacttca ggtttggaaa tgagccaaaa cgccatccgc ctgtcttgga ctcgtgaaga
     3001 agtcgaccaa cgcctgttcg gcatcatgca aagcatccac gaatcctgtc tgaaatacgg
     3061 caaagtcggc gacacagtaa actacgtcaa tggtgcgaac attgccggtt tcgtcaaagt
     3121 tgccgatgcg atgctggcgc aaggcttcta agcaaacgcc gccgctccgc aaacaaaatg
     3181 ccgtccgaac cgcaaatgct gttcagacgg catttcatta tccgcccgtt cgaatcgggt
     3241 gatactattt gtatccagaa ttgttggata gtttgggagt ggagaggaag aaatgattat
     3301 ggaccaatac tatatggagt taaagaataa gttatctaac agaccaatcc tactggata
//
LOCUS       DAXHKX010000195         3338 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXHKX010000195 DAXHKX010000000
VERSION     DAXHKX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.195"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(162..800)
                     /locus_tag="ACFMWZ_001943"
     CDS             complement(162..800)
                     /locus_tag="ACFMWZ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC5935843.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            1055..1897
                     /locus_tag="ACFMWZ_001944"
     CDS             1055..1897
                     /locus_tag="ACFMWZ_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC5935844.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            1963..2625
                     /gene="exbB"
                     /locus_tag="ACFMWZ_001945"
     CDS             1963..2625
                     /gene="exbB"
                     /locus_tag="ACFMWZ_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC5935845.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            2628..3062
                     /locus_tag="ACFMWZ_001946"
     CDS             2628..3062
                     /locus_tag="ACFMWZ_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC5935846.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
BASE COUNT          878 a          780 c          941 g          739 t
ORIGIN      
        1 cagactggag tttcttaatc agtgtttcag ttaatgaaag gtattatcat gcaacttggt
       61 tctaataatg atgaaaaaaa taagattaca ctacaggaaa acgcggcagg gcaatatgtc
      121 ctgccgcgtt ttctggagcg tttatgccgt ctgaaagcca gtcagacgaa catctgcaca
      181 aacgcaatca ccagccgcac aatcggtgca ataaacgcac ccaaaacccc ggtcagcata
      241 agcagcagga taatccacgt cccataaggt tcgattttgc ggaacgcttg cgaatatttc
      301 gccgacagga aggtgtcgat gaaaatgccg ccgtcccaag gcaggatggg gatgatgttg
      361 agcgcgaaca gaatcgcatt gatcagaata ccgtagtttg ccatttgagc caacggcatc
      421 tgatacgccc cgccgacata cggagtcagc accaaaacca cgccccacag aacagccatc
      481 gccagattcg acagcgggcc ggacgcggca acgcaacgcc aggcaaggcg cgggttgcgg
      541 aagttgcgcg aatcgatagg aatcggacgc gcccagccga acaggaaggg cgtgaacatc
      601 aaagtaagca gcggtacgat gattgtgccg accaaatcga tatggggcag ggggttcagt
      661 gtcagcctgc cgtattgttc ggcagtgttg tctccccagt agcgcgccgt atagccgcgc
      721 gccacctccc tgacggtaat ggcgagcagc acgggcagga cggcaagcag aaacacgccc
      781 aaatcaaaat tttgaaacat ggttttcctt tgaacagatg tggtgtgcag cccgaaacgg
      841 ctgacagaca gaagcggcaa tatatcatct aacctgattt tattggaata agaaatcaga
      901 cggaatcatt ttttctgccg gcccgttatc tgatgatttg ctgtaataca gcgtttaaac
      961 acggtttgcc tgtgaacgga acagacattc atatgaccaa aaattgtaaa aataggttgc
     1021 aaataggaat tgttgctatt attatgcccg aattatggat aaagaacgaa ttttaacccc
     1081 cgcagtcgtg ttttccgtcg cactgctgca tttggcaatg gtggcattgc tttggcaggc
     1141 gcacaagctg cccgtgatag agtcaggcaa tgttattgaa tttgtcgatt tgggtgattt
     1201 tggcggaggg gacggtgcac ccgaaggcgc aggtgcgcct gctgcgcctg agccgcaacc
     1261 tgagcctgag ccgcccaaac ctgtcgagcc gcccaagccg gttttgaagc cggtggttac
     1321 gaaaaaggcg gatgcggata ttcagcagcc taaggaagag ccgaaacctg aagaaaagcc
     1381 gaaacccgaa gaaaaaccga aaccagagcc taagccggaa gcgaagcctg tcccgaaacc
     1441 ggcggaaaaa ccggtcgaga agccgtctga aaaacctgcc gaacatccca gcaatgcttc
     1501 tgccaaagca gacagcgagc agggtaatgg ggaagataag ggaaccggta ccaaaggaga
     1561 cggaacgggg cgcggagaag gcagcggtaa aggtagcggc ggtgtcaaag gcgaacacgg
     1621 ggaaggagcc ggcagcagca aaggcaatcc tttacgcgcc aacggcagca ttccgcgccc
     1681 ggcttatccc acgctttcta tggagaatga cgagcagggt acggttgttt tgagcgtttt
     1741 ggtttctccg ggcggtcatg tcgagtccgt taaaatcgtg aaaagcagtg gtttttctcg
     1801 gttggacaat gcggcacgca aggcggcgca aaacgggcat tttcaagcca atgcctggac
     1861 ggagtttaaa gtccccgtca agtttgaatt gaattaggcg gttgccgcag cggtttgcgg
     1921 cagcttggat gtttgattgg ttttttcagt gttggaatca ttatgaattt gaaattagta
     1981 tttgaatcgg gcgatcccgt cctgatcggt gtgtttgtgt tgatgctgtt gatgagtatc
     2041 gtaacgtggt gtttggttgt cttgcgctgc atcaagctgt atcgggcgcg caaagggaat
     2101 gccgccgtca aacggcatat gcgcgatact ttgtcgctga acgacgcggt cgaaaaagtg
     2161 cgcgccgtcg atgcgccttt gtccaaactg gcgcaagagg cattgcagtc ttaccgcaac
     2221 taccgccgaa acgaagcgtc cgaactggcg caggctttgc cgttgaacga gtatttggtc
     2281 attcaaatcc gcaacagtat ggcgcagatt atgcgccggt ttgattacgg gatgaccgcg
     2341 cttgcctcca tcggcgcgac cgcgccgttt atcgggttgt tcggcacggt ttgggggatt
     2401 taccacgccc tgatcaatat cgggcaaagc gggcagatga gtattgcggc ggttgccggc
     2461 ccgattggcg aggcactggt ggcgacggcg gcgggtttgt tcgtggcgat tccggcggtg
     2521 ttggcataca acttcctcaa tcgcggcaca aaaatactga cccaggattt ggatgcgatg
     2581 gcgcacgatt tgcacgtccg cctgcttaat caaaaggata gctgattatg gcatttggtt
     2641 cgatgaattc cggcgacgat tctccgatgt ccgacatcaa cgttacgccg ttggtggacg
     2701 tgatgctggt gttgctgatt gtgtttatga ttactatgcc ggtgctgacg cattccatcc
     2761 ctttggaact gccgaccgcg tccgagcaga caaacaagca ggacaaacag cctaaagacc
     2821 ccctgcgcct gacgattgat gcgaacggcg gctattatgt cggcggggat tctgcaagca
     2881 aagtggaaat cggggaagtg gaaagccgtc tgaaagccgc caaggagcag aatgaaaacg
     2941 tgattgtggc gattgcggca gacaaggcgg tggaatacga ttatgtaaac aaagctttag
     3001 aagccgcccg tcaggcagga atcaccaaaa tcggttttgt aaccgaaacc aaggcgcaat
     3061 aaagttcgac gcttgaacga atgccgtctg aaagtctttc agacggcatt tcttatgttt
     3121 aatcagctat atatagttga ttaaaacaaa aatgagacga cagagcgccg tctccatttc
     3181 tgcgcagacg ggaatacaaa catctgattt ttcttttatt ttcaaacatt actgaaatgg
     3241 gtaagtctgg agtcctgcct gcgcgagaat aatgaaaatg tgcatttcta atttttatag
     3301 tggattaaca aaaatcagga caaggcaacg aagccgta
//
LOCUS       DAXHKX010000196         3296 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXHKX010000196 DAXHKX010000000
VERSION     DAXHKX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.196"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            99..1214
                     /gene="lst"
                     /locus_tag="ACFMWZ_001947"
     CDS             99..1214
                     /gene="lst"
                     /locus_tag="ACFMWZ_001947"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC5935847.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            complement(1378..1836)
                     /locus_tag="ACFMWZ_001948"
     CDS             complement(1378..1836)
                     /locus_tag="ACFMWZ_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC5935848.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            1955..2776
                     /locus_tag="ACFMWZ_001949"
     CDS             1955..2776
                     /locus_tag="ACFMWZ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC5935849.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQVLADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFGDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2827..3273)
                     /gene="yciA"
                     /locus_tag="ACFMWZ_001950"
     CDS             complement(2827..3273)
                     /gene="yciA"
                     /locus_tag="ACFMWZ_001950"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC5935850.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIAWGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
BASE COUNT          781 a          703 c          947 g          865 t
ORIGIN      
        1 tattcggtaa ctgtcggaat atctgctaaa attccgcatt tttccgcccg ggacactcgg
       61 ggcgtatgtt taatttgtcg gaatggagtt ttagggatat gggcttgaaa aaggcttgtt
      121 tgaccgtgtt gtgtctgatt gttttttgtt tcgggatatt ttatacattt gaccgggtaa
      181 atcaggggga aaggaatgcg gtttccctgc tgaaggacaa actcttcaat gaagaggggg
      241 aaccggtcaa tctgattttc tgctatacca tattgcagat gaaggtggcg gaaaggatta
      301 tggcgcagca tccgggggag cggttttatg tggtgctgat gtctgaaaac aggaatgaaa
      361 aatacgatta ttatttcaat cagataaagg ataaggcgga gcgggcgtat tttttccacc
      421 tgccctacgg tttgaacaaa tcgtttaatt tcattccgac gatggcggag ctgaaggtga
      481 aggcaatgct gctgccgaag gtcaagcgga tttatttggc aagtttggaa aaagtcagca
      541 ttgccgcctt tttgagcact tacccggatg cggaaatcaa aacctttgac gacgggacag
      601 gcaatttaat tcaaagcagc agctatttgg gcgatgagtt ttctgtaaac gggacgatca
      661 agcggaattt tgcccggatg atgatcggag attggagtat cgccaaaacc cgtaatgctt
      721 ccgacgagca ttacacgata ttcaagggtt tgaaaaacat tatggacgac ggccgccgca
      781 agatgactta cctgccgctg ttcgatgcgt ccgaactgaa agcgggggat gaaacgggcg
      841 gcacggtgcg gatacttttg ggttcgcccg acaaggagat gaaggaaatt tcggaaaagg
      901 cggcaaaaaa cttcaacata caatatgtcg caccgcaccc ccgccaaacc tacgggcttt
      961 ccggcgtaac cacattaaat tcgccctatg tcatcgaaga ctatattttg cgcgagatta
     1021 agaaaaaccc gcatacgagg tatgaaattt ataccttttt cagcggcgcg gcgttgacga
     1081 tgaaggattt tcccaatgtg cacgtttacg cattgaaacc ggcttccctt ccggaagatt
     1141 attggctcaa gccggtgtat gccctgttta cccaatccgg catcccgatt ttgacatttg
     1201 acgataaaga ttaatcgcat agcaaatcaa aatagaaaat ggcggagtaa gtaaggcaaa
     1261 aatcaggata tggcgtattt tttgaattga cgataatttc tgattgcttt gcgtgtgctg
     1321 aaatggcaaa gaaaatgccg tctgaaggct tcagacggca ttgttctgtt tcggatgtta
     1381 ttcgggtgcg cggaaactgt cgtggcagga tttgcagctt gcgccggttt cgccgtaggc
     1441 ggctttgatt tcatccagtt tgccggtttg tgctgcggca ttgagttttt cgacggcggc
     1501 ggcgaatttt gttttttcgg cttcaaattt tgcaccatcc gaccaaacgg ctggcagggc
     1561 gcggccgttg ccttgcggat cggactcaaa aagtgtgaac ggtttcttgc tgctttcggc
     1621 aaacgccgcg gcagcctgtt tgaatttttc gacatcgtaa ggctcttcgc ctttgaccat
     1681 tttgcccata cgcgagaagt cgggcatcat agatttgaac gcggcggtgc ggttttcgga
     1741 aatttcgcct ttgggttggg aaggtattcc gctgcctccg caggcggaaa ggagcagtgt
     1801 gatggcggca gcagcaaggc tgatttgggt tttcatattg aatgtgtcct gtcgtggtgg
     1861 tatggttgtt gtcattttca gtcggcgcga tgccgtctga agggtgttat gatacctgaa
     1921 acaggtttgg gaaacgagaa agaaaggaaa aacaatggct gttttaatta ccggtgcttc
     1981 ggcaggattc ggcgaagcga tgtgccgcgc gtttgtcggg gctggatacc gcgttatcgg
     2041 tgcggcgcgc cgtgcggaca ggcttcaggt tttggcggat gaattgggtg ctttgtttta
     2101 ccctttggaa atggacgtgt cgcgacgcga gtcggtggaa aatgccttaa acggcatccc
     2161 cgatgaattt tccgacatcg actgcctcat caacaatgcc gggctggctt tgggtttgga
     2221 cacggcggac aaggcggatt ttggagattg ggaaacaatg attcaaacca atgttttggg
     2281 tttgacgttc ctgacgcgca agattttgcc gcaaatggtg gaacgtggcg gcggctatgt
     2341 gatgaatttg ggttcgattg cgggtaatta tgcttatgcc ggcagcaacg tttacggggc
     2401 gaccaaggcg tttgtgcgcc agttcagcct gaatttgcgc gcggagttgg cggataagaa
     2461 catccgcgtt accaatatcg agccgggttt gtgcggcaat acggagtttt ccaacgtgcg
     2521 cttcaaaggc gatgacgaga aggcagcagg cgtgtatgag ggtgtggaat ttatccgccc
     2581 cgaagatatt gcggaaaccg cattgtggct gtaccgccgg ccggcgcata tgaatgtgaa
     2641 cacgattgaa attatgcccg tggcgcagac ttttgcagga atgaaagtga taaaaaaagc
     2701 cgtgcccgaa gtgcgggaag actttgaaaa acagagtatg tcgctgtttt cccgcatcag
     2761 gtcctggttc aaatgatgcg gaatgccgtc tgaagacagt ttcagacggc atttttacgg
     2821 gtatttttac ggagtaggca ataagcccga caatttgggg ttgccttctt tcggaatcgg
     2881 gcgcggattg ccttccgcat cgatggcaac ataagtgaac accgcctcgg ttacgaggta
     2941 gcggtcttcg gtaacgcaat cgttcatcaa agttttcacc cagacttcga ttttaagctg
     3001 gagggaagta ttgcccacgc ggacgcaatg cccgtagcag cagacgacgt tgccgacctt
     3061 gaccggacgg atgaagttca tttcctgaac ggcgacggta acgatgcgtc cccacgcgat
     3121 ttccgccgcc aatatgccgc cgcccaaatc catctgcgac ataatccagc cgccgaaaat
     3181 gtcttggttg ggattggtat cgcgcggcat agcgacggta cgcaggagca gttcgccttg
     3241 agggcgttgg cggttgcctt cttcgtgctg cataaagttt ccttgtttta ttgaaa
//
LOCUS       DAXHKX010000197         3294 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXHKX010000197 DAXHKX010000000
VERSION     DAXHKX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.197"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(181..1281)
                     /locus_tag="ACFMWZ_001951"
     CDS             complement(181..1281)
                     /locus_tag="ACFMWZ_001951"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC5935851.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADTHQMYICGGGIRNPVLMADLAECFGTRVSLHSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1606..3180
                     /locus_tag="ACFMWZ_001952"
     CDS             1606..3180
                     /locus_tag="ACFMWZ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC5935852.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          687 a          819 c          969 g          819 t
ORIGIN      
        1 atgccggtca gtgtgccgca ggcggttagg acgaggggga tgaggaggag cagggttttc
       61 atggcagggt ttgttttgtt ttgaacgagt ttgagtgtaa atggatttta agggtttgta
      121 aacaaaaggg gcgaaaatgc cgtctgagcg gcggaaatgg ctttcagacg gtatttgcgc
      181 tcaataataa tatcccgcgc ccagaataca cggtttggat gcgccggttg ctttgtgcgg
      241 actaccggga atgcgattaa tccaacacgc cgccaaccac gcaaatgcgg cggcttccac
      301 ccattgcgga tcgaggttca ggtcggcggt gctgtgcagg gaaacgcgtg tgccgaaaca
      361 ttctgccaaa tccgccatta aaacaggatt gcggatgccg ccgccgcaaa tatacatttg
      421 atgggtatct gccgcagcgt gtgagactgc gtcgcaaacg gtttgcgcgg taaaacggga
      481 aagcgtccgc aatacgtcgt atcggttttc gccgccgtca aggtaggttt cgagccaatt
      541 tagggcaaac agttcgcgcc ccgtgctttt agggtggggt tgtgcgaaat acgggtgggc
      601 gagcagcctg tcgagcagtt gcggcaatat gttgccttgt gccgcctttg caccgttttt
      661 gtcgtaaggc agctgccagt gtgcctgcgt ccacgcgtcc atcagcatat tgcccggccc
      721 cgtgtcgaag ccgaaggcgg gtgcgtcgga ggggagtacg ctgatgttgg caatcccgcc
      781 gatgttcagt accacgcgtg tttctttgtc atcgcggaac agggctttgt gaaaggcggg
      841 gacgagcggc gcaccttgtc cgccggccgc aaggtcgcgg ctgcggaagt cgccgacggt
      901 aaaaatctga gtcagttccg ccagcagcgg caaatcggca agctgtatgc tgtaaccgtg
      961 ttccggcgcg tgtcggacgg tttgcccgtg gcagccgagg gcggtaatgt cggacggcgc
     1021 gaggttttga ctgcacagca gttcggcggc ggtttgcgcg tacaggcggc tgagttcttg
     1081 cgacaaaatc ctgctgcggt gcagttcgtc cgcccccgtg tcctgcaaat ccagcaattg
     1141 gcggcgcagc ctgtcgggat agggggtaaa ggcgtgccct tccgcgccca gccatttgcc
     1201 gccgtccatc cgtaccagca cggcatccgc cccatccata ctggttcccg acatgatgcc
     1261 gatgtaaagc tgtgtttcca tcatcactcc caaactggtg caaaacgcca ttttaacgtg
     1321 tattgacgct cgtataccga tttgccgccg cagtgtaaat aaagtgtaaa taaatgtttc
     1381 aagacggatg gaaaaatatt ataatgcgcc cgcaacatcc agtagtagaa gtgtcataca
     1441 aaccgtttcc ggcagcagtt ttgcattcgg tcaggtttgg gggtattcgg atgcggttca
     1501 gaaggaagga tgcgtctgcc atatcccgaa acggcagttc gaccggaggc agcagtacag
     1561 tgtcggcaac actcatgatt tccaccacat taaaggaaga ttgccatggc tcaaatccaa
     1621 atgagcgcaa atgttaaaac catcaacgcc gtctttgccg ccatgctggt aggtacagtc
     1681 ggctatttta tttattgggg cttgggttat acccattaca attacgccgc cttattcatt
     1741 attgccacga tgttcggcgt gtttatggcg ttcaacatcg gcggcaacga tgttgccaat
     1801 tctttcggca ccagcgtcgg tgcgggtacg ctgaccatcc cgcaggcttt gctgattgcg
     1861 gcggtatttg aggtcagcgg cgcggtcatc gcgggcggcg aggtaaccaa taccatacgc
     1921 aaaggcatcg tcgatttgaa gggtgttgat ttcgaaccca tacagtttgt gtttattatg
     1981 atgtccgcgc ttttggcggc ggcgttgtgg ctgttgtttg cctcgaaaaa agggcttccg
     2041 gtatctacca cccattccat tatcggcggc attgtcggca gcgcggtatg tatggcggta
     2101 atgaacgatg ccgcatcggg cgatttgata cgttggggca agctgggcgg tattggtgtt
     2161 tcttgggtat tgtcgcccgt gttgggcggc gcggtgtcct atttcctgtt ttcgcgcgtc
     2221 aagaaaaacg tcttagatta caacgcttgg gcggaaggca cgctcaaggg catcaagcag
     2281 gaaaaaaagg cctataaaga acggcaccgc ctgtttttcg agggtttgtc cgaagccgaa
     2341 aaagtcgagt acgccaccaa aatggcgcac gacgcgcaaa tttacgacga acccgaattc
     2401 gatccgcaag agctgcaatc ggagtattac cgcggtcttt atgcgttcga caaccgtaaa
     2461 aacaatgtcg attcctacaa agcactgcat tcttggattc cctttatcgc ttcgttcggc
     2521 gcgatgatga tttccgctat gctgattttc aagggcttga aaaacctgca tttggggatg
     2581 agcaacgtca acagcttcct gaccatcttt atgataggcg cggcggtgtg gatggggacg
     2641 tttgtgtttg ccaaaagcct caagcgtaaa gacttgggca aatcgacctt tcagatgttt
     2701 tcatggatgc aggtctttac cgcctgcggc ttcgcattca gccacggggc gaacgatatc
     2761 gccaacgcca tcggtccgtt tgccgcgatt atggatgttt tgcgtaccaa cagcgttgcc
     2821 gcgcaaaatg tcgtcccccc gattgcgatg ctgactttcg gcatcgcgct gattgtcggt
     2881 ttgtggtttg tcggtaaaga ggtgattaaa accgtcggta cgagtttggc ggaaatgcac
     2941 cccgcctcgg gttttaccgc cgaactgtcc gccgcatccg tcgtgatggg cgcgtcgctg
     3001 atggggctgc ccgtgtccag tacgcatatc ttggtcggcg cggtactcgg catcggtctg
     3061 gtcaaccgca atgccaactg gaaactgatg aagcccatcg gtttggcgtg ggtcattacc
     3121 ctgcctgccg ccgccgtatt gtcggttgtc tgctacttgg ttttacaggc agtattctga
     3181 ttgtaaaata ctgatgccgt ctgaacccgt gttcagacgg cattttgttg atggaatgtg
     3241 cgggcttgtg ccttatgcac aatctgttct gtcgggatat gccgtttggt atag
//
LOCUS       DAXHKX010000198         3266 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXHKX010000198 DAXHKX010000000
VERSION     DAXHKX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.198"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            166..1278
                     /gene="nadA"
                     /locus_tag="ACFMWZ_001953"
     CDS             166..1278
                     /gene="nadA"
                     /locus_tag="ACFMWZ_001953"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC5935853.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGNNEI
                     LLDKKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1330..2838
                     /gene="nadB"
                     /locus_tag="ACFMWZ_001954"
     CDS             1330..2838
                     /gene="nadB"
                     /locus_tag="ACFMWZ_001954"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC5935854.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCNEAAVRTILSQGKPAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIVQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYNIRARHTVLAGGGLGQIYASTTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAEISKQTQDFVSLDISRQPAEFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPGGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTAKLAAQT
                     IADGQAFQTAPPQRPSENPFSEANIFSDGIQNTFSRPALQAFNQRHLGILRNDTGLRR
                     AIAQLRLWKQNQTEPHTVSEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            3021..>3266
                     /locus_tag="ACFMWZ_001955"
     CDS             3021..>3266
                     /locus_tag="ACFMWZ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC5935855.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLI"
BASE COUNT          774 a          999 c          874 g          619 t
ORIGIN      
        1 accaaagcgg tttgtcggaa cgaagcgggt cggttttacg gtatcgcaaa cctgttttca
       61 gacgaccttg cttattatac tcaatttgag cataatataa aaatatttca aaatataaac
      121 ggcaaatctt tatgccgtct gaaaaaccag ccataaggaa gtctcatgca aaccgccgcc
      181 cgccgctcgt tcgactacga tatgccactc atccaaacgc cgacttccgc ctgccaaatc
      241 cgtcaggcgt gggcgaaggt tgccgacacg cccgaccgcg agacggcaga tcgtctgaaa
      301 gacgaaatca aggctttgct gaaggagaaa aacgcggtct tggtggcgca ttattatgtt
      361 gatccgctga ttcaggattt ggctttggaa acgggcggat gcgtgggcga ttctctggaa
      421 atggcacgct tcggcgcgga acacgaagcc ggtacgctgg tggtggcggg cgtgcgtttc
      481 atgggagaga gcgcgaaaat cctctgccct gaaaaaacgg tgctgatgcc tgatttggaa
      541 gcggaatgtt ctttggattt gggttgcccg gaagaggcgt tttcggcgtt ttgcgaccaa
      601 caccccgacc gcacggtggt ggtgtacgcc aacacttccg ccgccgtgaa agcgcgtgcc
      661 gattgggtgg taacgtcttc ggtggcgttg gaaatcgtgt cgtatctgaa atcgcgcggc
      721 gagaagctga tttgggggcc cgaccgccac ctcggcgact acatccgccg cgaaacgggc
      781 gcggatatgc tgttgtggca gggttcgtgc atcgtccaca acgaatttaa agggcaggag
      841 ctggcggcgt tgaaggcgga acaccccgat gcggtggtgc tggttcatcc cgaatcgcca
      901 caaagcgtca tcgaactggg cgacgtggtc ggctcgacca gcaaactgct gaaagctgcc
      961 gtatcacgcc ccgaaaaaaa attcatcgtg gcgaccgatt tgggcatcct gcacgaaatg
     1021 caaaagcagg cgcccgacaa agaatttatc gccgcgccga cggcgggcaa cggtggaagc
     1081 tgtaaaagct gcgcgttctg tccgtggatg gcgatgaatt cgctgggtgg tatcaaatac
     1141 gccctgacaa gcggaaacaa cgaaattctg ttggacaaga agctgggcga agccgccaaa
     1201 ctgcctttgc aacgtatgct cgacttcgcg gcagggctca aacgcgggga tgtgttcaac
     1261 ggtatgggac ccgcctgatt tgccgtccat attgccgttt cagacgacct ctcaaacaag
     1321 gacaacacca tgcaaaccga ttgcgacgta ttgattgccg gaaacgggct ggcggcactg
     1381 acgctcgccc tgtcgctgcc tgaatcgttc cgcatcgtca ttttgtgcaa aaaccggctg
     1441 gacgacactg ccagccgtca tgcgcaaggc gggattgcgg cggcgtggtc gggagaggac
     1501 gacatcgaaa aacacgttgc cgatactttg gaagcgggcg caggtttgtg caacgaagcc
     1561 gccgtccgca ccatcctgtc gcagggcaaa ccggcaatcg aatggctgct ggcgcagggc
     1621 gtggcgttcg accaaaataa taacggtctg cacctgacgc gcgaaggcgg gcacacctgc
     1681 cgccgaatcg cccacgtcgc cgactacacg ggcgaagccg tcatgcagag cctgattgtc
     1741 caaatacgcc gtcgcccgaa catccgcgtt tacgagcggc agatggcgtt ggacgttcaa
     1801 accgaatcgg gtgcagcgtg cggactgacc gtcctcgact gccgaacgca agaaacctac
     1861 aacatccgtg cccgccatac cgtactcgca ggcggcggct tgggacagat ttacgcctcc
     1921 accactacgc cgcccgaatg cacgggcgac gccatcgcca tggcgatacg cgcaggctgc
     1981 gcagtcgaaa acctcgaatt tatccaattc caccccacag gcttggcaag accgtctgaa
     2041 aacggacgca ccttcctcat ttccgaagcc gtgcgcggcg aaggcggcat cctgaccaac
     2101 caatcgggcg aacggtttat gccgcattac gaccgccgcg ccgaactcgc gccgcgcgac
     2161 atcgttgccc gcgccattgc cgccgaaatc tccaaacaaa cgcaagactt cgtctcgctc
     2221 gacatcagcc gccaacccgc agagttcgtc cgccggcatt tcccgtccat ccatcggcac
     2281 tgcctgtccc aatgcggctt ggacatcacg cgacaagcca tccccgtccg ccccgttcaa
     2341 cactacacct gcggcggcat ccaaaccgac cccggcggca gaacctccct gccgcagctc
     2401 tacgccttag gcgaaaccgc ctgcacaggg ctgcacggag ccaaccgcct cgccagcaac
     2461 tccctgctcg aatgcgtcgt gaccgccaaa cttgccgccc aaaccatcgc ggacgggcaa
     2521 gcgttccaaa ccgcaccgcc ccaaaggccg tctgaaaacc cattctctga agcaaacatc
     2581 ttttcagacg gcatccaaaa cacattcagc cgccccgccc tgcaagcgtt caaccaacgc
     2641 catctcggca tcctccgcaa cgataccggc ctgcgccgcg ccatcgccca actgcggctt
     2701 tggaagcaaa accaaaccga accgcacacc gtgtccgaat acgaaaaccg caacctgctc
     2761 gaatgcagcc tcgccgtcgc ccaagccgca tacaggcgga gacagaacat cggcgcgcat
     2821 tttaatagtg attgttaagg ttggctggtt agaaaaaatc gttcaaatga attttaatag
     2881 catggttaag agattggcat tcagttttta gggttgcgta agttgggtta tcaatctgtt
     2941 ctaaaagcag aaaggccgag acctttgcaa aattccccaa aatcccctaa attcccacca
     3001 agccatttag gggattttcc atgagcacct tcttccggca aaccgcacaa gccatgattg
     3061 ccaaacacat cgaccgcttc ccgctattga agttggacca ggtgattgat tggcagccga
     3121 tcgaacagta cctgaaccgt caaaaaaccc gttaccttcg agaccaccgc ggccgtcccg
     3181 cctacccgct gttgtccatg ttcaaagccg tcctgctcgg acaatggcac agcctctccg
     3241 atcccgaact cgaacacagc ctcatt
//
LOCUS       DAXHKX010000199         3200 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXHKX010000199 DAXHKX010000000
VERSION     DAXHKX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.199"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            265..1044
                     /locus_tag="ACFMWZ_001956"
     CDS             265..1044
                     /locus_tag="ACFMWZ_001956"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC5935856.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1249..2634)
                     /locus_tag="ACFMWZ_001957"
     CDS             complement(1249..2634)
                     /locus_tag="ACFMWZ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC5935857.1"
                     /translation="MIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGRLHAHK
                     VKAELVRHYGVDCYVSAKNLLKTFTTVRFEKGSDGILNAKTADLKNGVTDNPPTGLFA
                     NKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACGRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKDLPDAFKAKF
                     KTDRKNIKEIG"
     gene            2752..2828
                     /locus_tag="ACFMWZ_001958"
     tRNA            2752..2828
                     /locus_tag="ACFMWZ_001958"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2786..2788,aa:Arg,seq:acg)
     gene            2846..2920
                     /locus_tag="ACFMWZ_001959"
     tRNA            2846..2920
                     /locus_tag="ACFMWZ_001959"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2879..2881,aa:Glu,seq:ttc)
BASE COUNT          890 a          950 c          590 g          770 t
ORIGIN      
        1 tgtaatatgc aagtgtgcaa atcgtccaaa ggccctcaca actgttttga ttttttatat
       61 taattgaaaa aaagtaattc tcaattaaat ttatagatgg gattgtattc ccattttgac
      121 aaaaaacaaa ctgctcctta ccgtttattt caaaaaacga taatattgta ttgaaaaata
      181 tccgaattta aatacagatc gtcaatgcgg aaaaaaacac ccaaattggc tataatcccg
      241 acaaacacac tcaaggacaa caacatggca gcctcgcccg aagcaaaatt cactgaagaa
      301 aagattttgt gggtcaaaca ccacacgccg aaactcatca ctttcgccat cagccgtccc
      361 gaatcctacc gttttaaagc cggacagttc tcccgactcg gtttctacga aggggaaggc
      421 tttatttggc gtgcctactc catcgtttcc gcagaatatg cagacacgct cgaatatttt
      481 gccgtactca tccaagacgg ccccatgtcg gcccgtttcg ccaaaatgca acagggcgac
      541 accatcctgc tcgataaaaa tgccaccggc ttcctcctgc ccgaacgctt ccccgacggc
      601 aaggatttgg tgatgctctg caccggctcc ggcatcgccc ccttcctttc cattctcgaa
      661 caacctgaaa tccgacagcg tttcgatacc gtcaacctga tacattccgt atcttttccc
      721 gaagaattga ttttcaacga ccggctcgcc gcattgtccg aacatcccct ggtaggcgaa
      781 tacagacact cgttccattt cgtccctgtt accacccgtg ccgccaaccc ctcgggctta
      841 agcggaaaac gcattccgga actcttaaaa aacagcagca tcgaacaggc gctgcatacc
      901 aagttcaccc cggaatccac acggtttatg atttgcggca acccggaaat ggtcaaagat
      961 actttccaaa cgctgcttga catgggttac gccatgcacc gcaaccgcat tcccggtcaa
     1021 atcatgatgg aaaacggctt ctaaaaacca ccctgcttgt ccgatgcctt cggatggacg
     1081 gtcaaaccga cacggcacga aaaccgcgtc ggcaaaaatg ccgtctgaag ccttcagacg
     1141 gcattttcgg atacatacaa acggcaacat attccaaccc aaaccgatta ggcaatcaat
     1201 acgaacggct gtttacatac ttaccggctt tcaccaaccg atatcgattt aaccgatttc
     1261 cttaatattt ttcctgtccg ttttaaactt cgccttaaac gcatccggta aatctttatc
     1321 gaaataccaa agcccgtcat ccatttccaa tgcgccgccc attccatgca gaacaacttt
     1381 ttcccccgtt aaaggatcgg tttcggtaat ttccacctca tgcatttttc cccaatccaa
     1441 aacaggcaac ttgtgtgcaa acgccaaaac ctgttcgtca gaacggccgc acgccttatc
     1501 gcatcggcct gaaacatata cgggacaagg ctcatcctct gcataaccgt ccggcaggat
     1561 accggctgcg gcaggacaca atccgtatgt ttccgcccac gacgacaaag cccgtctcgc
     1621 cgccttttta ttggcaaata atccggtagg cggattatcc gtcacaccgt tttttaaatc
     1681 tgctgttttc gcattcaata tgccgtctga acctttttca aacctgacgg tcgtaaatgt
     1741 tttaagcaga tttttggcag atacgtaaca atccacccca taatgcctga ccaattccgc
     1801 tttaacctta tgggcatgca gcctgcccaa tgcgggaaaa aaacggactt cctccgtatt
     1861 gcaccaatca aacggggctt ttccggagtc caataaagcc gaaacctcgc tatatacccg
     1921 ttcaaacgta ccgatgtaat ttactttccc tccgccgtcg aaacaggcca gcacccccat
     1981 accgtcaggc aaaccgtaca actgttccct caaccgttcg ggcagcgcgg caggcaacgg
     2041 tttcggattc atcaaacgga aacactgcct gatccactcc tcgaccccgt gttccgacag
     2101 gctgtattcc aaataatcac acaacgccga cacatccgcc atcgcacgat gcctgtcttc
     2161 cacaacaatc cccaaccttt cgatgatact gtccaggctg tgcttgtaaa attgcggata
     2221 cagacaccgg gacagctgca cactgcacaa agcaggcgat gaaaatccga tacccgcacg
     2281 atgaaactca tgctttaaaa acgtatagtc gaaacggctg ttatgtgcaa ccagcacaca
     2341 acccttcaat accgaaaaca actcgccggc aatctctgca aaaacaggcg catcggcaac
     2401 catgccgtct gaaatccccg tcagccccgc cacaaactgc ggaatcggtt tttgaggatt
     2461 aaccaaccac tcatgcctca ccacccttcc ctgctcaaac ttgaccaaag ccacttcggt
     2521 taccctgtct tcatacagat tgccgcccgt cgattccaaa tcgaccacgg caaccggcat
     2581 tccaaaccgt aaaaatacct tttccagcaa gggccagcgg gaagcaacaa tcattttatt
     2641 ctctttaaat tcaaacaaca aaccgatatt ttacactttt aaggcatttc atccaacaaa
     2701 acaattgaca gaatccgatg attaccctaa aattcaaatc tttcttgcag cgcacccgta
     2761 gctcagttgg atagagtatc tggctacgaa ccagagggtc gggcgttcga atcgctccgg
     2821 gtgcgccagt aagaaaatac aatatgcgcc catcgtctag cggttaggac atcgcccttt
     2881 cacggcggta accggggttc gattccccgt gggcgtgcca aattccaaat ccccgagatt
     2941 atccctcggg gattttttat tgtctcggca actcgttacc atatctttac ctaccccctt
     3001 catcagaatc tcagacacaa tcgaatcata ttcaaacctt tgccgtgcaa accgatatcc
     3061 cataaccaga tgcggtgtcc gtccaacatt ttacccgatt gaaacgcctg atatattgca
     3121 ccccatcaac gtggcattac ttttcttaac aatccccttt gacagcaact gactagggct
     3181 tttttatgcc atcatcaaat
//
LOCUS       DAXHKX010000200         3131 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXHKX010000200 DAXHKX010000000
VERSION     DAXHKX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.200"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            205..1254
                     /locus_tag="ACFMWZ_001960"
     CDS             205..1254
                     /locus_tag="ACFMWZ_001960"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC5935858.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKGETRKTATQAY
                     GQKASDGIKPQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(1323..2864)
                     /locus_tag="ACFMWZ_001961"
     CDS             complement(1323..2864)
                     /locus_tag="ACFMWZ_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC5935859.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFILGSLL
                     YFAVPSIGESGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYGVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDGETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT          796 a          919 c          829 g          587 t
ORIGIN      
        1 ttgaaagatt ctacccccgc acgcaaaccg atttcaaccg ttgcacaaac tttgcctcgc
       61 cacaagccgc agcgacgatt tcatccgcaa aaccgccgca tcaggtacaa tatcgaaccg
      121 tccgactgag gacggcattt tatcaacccg tcctgccgca tacgccgcag aagaaccgct
      181 ttatcaagcg agttaggaaa aatgatgtcc aaacagccca ccagcaaacg ccaatggcgc
      241 gacggcgcag ccccgtctgc caagaaaacc gccaaacctt tcaaaagcaa agcccgtccc
      301 aaaggcgaaa cgcgcaaaac cgccacccaa gcttacggac aaaaagcttc agacggcatc
      361 aagccgcaaa acgcccccaa acagcgcgcc gccaaagcca aaaaactcgt cgtccgcaat
      421 cccaaccaaa aaattatgga acacgcgcgc gatttgaaag aacgccgcag cgacctgtcg
      481 cgcatggaac ccgaacgcct gcaaaaagtg cttgccgcgt ccggcgtcgg ctcgcgccgc
      541 gaaatggaag aatggattac caacggctgg ataacggtca acggcaaaac cgcgcaactg
      601 ggcgacaaag ttacccccga cgaccacgtt accgtcaaag gcagcatcat caagctcaaa
      661 tgggcggacc gcctgccgcg catcatcctg tattacaaac aagaaggcga aatcgtttcc
      721 cgtgacgacc cgcaaggccg cgtcagcata ttcgaccgcc tgccgcaggc cgccagcagc
      781 cgctgggtcg ccatcggacg cttggacatc aacaccagcg gacttctgat tcttaccacc
      841 tccggcgaac tcgtccaacg tttcgcccac cccagcttcg aagtcgaacg cgaatacgcc
      901 gtgcgcgtct tgggcgggct gaccggcgaa caaatgcgcg tcctcaccga agaaggcgtg
      961 atgctcgaag acggcttggc aaaagtcgaa cgcatccgcg aacaaggcgg cgaaggcgcg
     1021 aacaaatggt acaacgtcgt gattaaagaa ggccgcaacc gcgaagtgcg ccgcattttt
     1081 gaaagccaag gactcaccgt cagccgcctc gtgcgcgtcg gcttcggtcc catcggactg
     1141 cccaaccgcc tcaaacgcgg gcagttctac gaactcaacc ccgccgaagt cgccaacatc
     1201 ctcaaatggg cggaaatgct gctgccgggc gaataccgcc gcaaaaaagc ctaaacccgc
     1261 caaaacacaa aaatgccgtc tgaaacatct gctgtttcag acggcatttt attcgggcgt
     1321 tttcaggaga aaaggtcgag tgctttgaca aagaccatca ccacgccgta ggcgagtggt
     1381 atgacggcca atgcccaacg ccaccagacc gatatgccgc cgccggaaac tttttcgccc
     1441 acaaggtaag cgtcggatat ggcggtttcg ccgtcggggt tgccgctgtg tgcggcggtt
     1501 ttgatgtctt tttcgtggtg tttttcgtgt acggatttga cggcgaggtt gcacaacaaa
     1561 ccgataatca gcaggcacgc catgatgtac atggttacgc cgtatgcctg tgccgccggt
     1621 atgccgctgt cgatttggct ttggcgtatg taattgacca gtaccgggcc gatgacggcg
     1681 gcggttgacc aggccagcag gatgcgcccg tgaatcgcgc cgacctgata ggtgccgaac
     1741 aggtctttca ggtaggcggg aatggcggca aatccgccgc cgtacatgga aataatcacg
     1801 caaaagccga tgatgaacag ggctttgctg ccgctctcgc cgatggaggg aacggcgaaa
     1861 tacagcagcg aaccgagtat gaagaagatg gtgtaggtgt ttttgcgtcc gattttgtcg
     1921 gaaacgctcg accacaaaaa gcgcccaccc atattaaaca ggctcaggag gctgacgaag
     1981 cctgccgccg cacctgcgcc gactgccgcc tgcctgccta cggaggtttc ggaaaagagt
     2041 tcctgaatca tcacggatgc ctgacccaat acgccgatgc cggcagttac gtttaggcac
     2101 aatacccaga acaacagcca aaactgcggc gttttcatgg cttgggacac gttgacatga
     2161 ttgctgctga ccagcttgtt ttgcgttttc ggcgcggtat agccttcagg tttccagccg
     2221 tcggcaggta cgcggatggt aaacgcgcca aacatcatca gtgcgaggta aagcagaccc
     2281 aatacggcga aggtttcggc aaccccaacc gaagcagcgt ttgaaaaggc gttcatcagt
     2341 gatacggaaa gcggcgaggc cagcattgcg ccgccgccga aacccataat cgccaaaccg
     2401 gtcgccatac ccggcttgtc gggaaaccat ttcatcagtg tggaaaccgg cccgatgtag
     2461 cctaagccta agcctacgcc gccgatgacg ccgttgccca aatagagcag gaagaggttg
     2521 tgcgtacgca cgccgaatgc ggatacgaag aagcctaggc tgaaacagca ggcggcggca
     2581 aatatggctt tgcgcggccc tacccgttcc atccacgtac cgaacagggc ggccgacgcg
     2641 cccagcatcg cgagtgcgat actgaaaatc caacctacgg tcgtcagctt ccaatctccg
     2701 gccgccgatt cggttatgcc gataagtttg gtcagcggcg cgttgaatac ggaataggcg
     2761 taaatctgcc cgatggcaag gtgtaccgcc aatgctgcgg gcggtacgag ccaacggttg
     2821 aaacccggct tggcaatgct tgcctcacgg tctaaaaact tcataacatc ctctttctgt
     2881 cagttgaaaa ataaaatttc atttgcccaa tggaaactta ttgaaaatta taaaaaaata
     2941 tcggatcggt tttttatccg ccccaagatg ccccgtctga aacatttcgg gtgtacggaa
     3001 aggtttctgt tttttccgac aaattcctgc ggcttttcgc ttccggattc ccgcttttgc
     3061 gggaatgacg aattaaagat tatcttaagg tcaagggact ggattcccgc ttttgcggga
     3121 atgacggcgg c
//
LOCUS       DAXHKX010000201         3119 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXHKX010000201 DAXHKX010000000
VERSION     DAXHKX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.201"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            45..2303
                     /locus_tag="ACFMWZ_001962"
     CDS             45..2303
                     /locus_tag="ACFMWZ_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC5935860.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSIDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDDRSYIATQTVTNPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEAEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            2316..2981
                     /gene="pgmB"
                     /locus_tag="ACFMWZ_001963"
     CDS             2316..2981
                     /gene="pgmB"
                     /locus_tag="ACFMWZ_001963"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC5935861.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVSEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKASGALPIGVGKAEDLGSDIPLVSGTAELTYAYLQNVWAQ
                     SGR"
BASE COUNT          799 a          955 c          787 g          578 t
ORIGIN      
        1 gatttcacca ttccctcaaa tcaatccaaa caggagcttc ataaatgtac acaagaatta
       61 tggaaatcag cccttggacg ctgcgttcgg caaagctgga aaaagaacac aaacggctgc
      121 aagagagcct gaccagtttg ggcaacggct atatgggtat gcgcggcagc tttgaggaaa
      181 cctactccgc cgacagccac ttgggcacct acatcgccgg cgtgtggttc cccgacaaaa
      241 cccgcgtcgg ctggtggaaa aacggctatc ccaaatattt cggcaaagcc atcaacgcgc
      301 tcaatttcag caaagtcaaa atctttgtcg atggtcaaga ggtggacttg gcgaaaaacg
      361 atgttgccgg cttctccgtc gaactcgata tgcagcacgg cgtgttgcgc cgctcgttca
      421 ccgtattcgg cgtgcgtttc gatgtgtgca aattcctgtc cgtcgcacaa aaagagctgg
      481 ctctcatccg ctgggaagcc gtatctatcg acggcaaaac ccaccaagtc cgcatcgatt
      541 ccatcatcga tgccgacgtg aaaaacgaag attccaacta cgaagaaaaa ttctggcaag
      601 tattggacaa aggcgtttca gacgaccgct cctacattgc cacccaaacc gtcaccaatc
      661 ccttcggcgt ggaacaattt atcgtcaacg ccgagcaaac tttcgccggc agcttcaaag
      721 ccctcggcgg cagccaaacc gactggcagg tctccaattc tttcgaggcc gaagtcggcg
      781 gcacgcccaa aacctttgaa aaacgggtta ttgttaccac cagccgcgat tatcagggct
      841 tggaagcagt gaaagccgca ggccgtgcct tgtcggaaaa agtcgcaggc gttgcgtttg
      901 aaaccttgct ggacgcgcac aaagcaggct ggctgcaccg ttgggaaatc gccgacgtgg
      961 tcatcgaagg cagcgacgaa gcgcagcaag gcatccgttt caatctgttc caactgttct
     1021 ccacctacta cggcgaagat gcgcgcctga acatcggccc caaaggcttt accggcgaaa
     1081 aatacggcgg cgcgacctat tgggataccg aagcctatgc cgtaccgctc tacctcgcac
     1141 tggcggaacc cgaagttacc cgcaacctgc tgcaataccg ccgcaaccaa ctgccgcagg
     1201 cgcagcacaa cgcgcgcgaa cagggcttgg cgggcgcact ctatccgatg gtaacgttta
     1261 cgggtatcga gtgccacaac gaatgggaaa tcaccttcga ggaaatccac cgcaacggcg
     1321 cgattcctta cgccatctac aactacacca actacaccgg cgacgagggc tatcttgcca
     1381 aagaaggctt ggaagtcttg gtcgaagtgt cccgcttctg ggcggaccgc gtccacttct
     1441 ccaaacgcaa cggcaaatac atgattcacg gcgtaaccgg cccgaacgaa tacgaaaaca
     1501 acatcaacaa caactggtac accaacaccc tcgccgcatg ggtattggac tacacccgcg
     1561 aagccttggc gaaatacccg cgtccggatt tgaacgtgcg tgccgccgag ttggaaaaat
     1621 gggcggacat cagcgcgaat atgtaccgtc cgcatgacga agaactcggc gtattcgtgc
     1681 aacacgacgg cttcctcgac aaagacatcc gccccgtgtc cgcgctttcg cccgacgatt
     1741 tgccgctcaa ccagaaatgg tcgtgggaca aaatcctgcg ttcgcccttt atcaaacagg
     1801 cggacgtatt gcaaggcatc tatttcttcg gcgaccgttt caatatcgac gaaaaacgcc
     1861 gcaatttcga cttctacgaa ccgatgaccg tgcacgaaag ctcgttgtcg ccgtgtatcc
     1921 actctattct cgccgccgaa ctgggcaaag aagaaaaagc cgtggaaatg taccggcgca
     1981 ccgcacgcct ggacttggac aactacaaca acgacaccga agacggcctg cacattactt
     2041 ctatgaccgg ctcgtggctc gccatcgtcc aaggtttcgc ccaaatgaaa acttggggcg
     2101 gtaaactcag cttcgcaccg ttcctgccga gcgcatggac aggctacgcc ttccatatca
     2161 actaccgagg ccgtctgatt aaagtcgccg tcggcaaaga aaacgtcgtc ttcaccctgc
     2221 tcaaaggcga gccgctcgat ttgcaggtgt acggcaaaga catcacactc aacggcagcc
     2281 ataccgctgc gttggaaaaa taaggagggc gcaaaatgac gtttaccgca gtgctcttcg
     2341 atctcgacgg cgtcatcacc gacaccgccg aataccacta ccgcgcgtgg aaaaaactcg
     2401 ccgaagaact gggcatcagc atcgaccgca agtttaacga gcagctcaaa ggcgtgtcgc
     2461 gcgacgattc gctcaaacgc atcctcgcgc acggcggcaa aaccgtcagc gaagccgagt
     2521 tcgccgaact gacccgccgt aaaaacgaca actacgtcga gatgattcag gcagtcaaac
     2581 ccgaagacgt gtatcccggc attttgcccc tgctggaaac attgaaagca aacggcaaaa
     2641 aaatcgccct cgcgtccgcc agtaaaaacg gcccgttcct gctcgaacgc atggggctga
     2701 cccacttctt cgacaccgtc gccgaccctg ccgccgtcgc gcattccaaa cccgcccccg
     2761 acatcttcct cgccgccgcc gagggcgtgg gcgcggacat ccgccgctgc atcggcattg
     2821 aagacgctgc cgcaggtgtc gccgccatca aagcctccgg cgccttaccc atcggtgtgg
     2881 gcaaagccga agacttgggt agcgacatcc cgctggtgtc cggcaccgcc gagctgacct
     2941 acgcctacct gcaaaacgtg tgggcacagt cgggcaggta aaacgcgtca gataaagtgt
     3001 taaggaagta aaaggccgtc tgaacagtgt ttcagacggc ctgagacctt tgcaaaattc
     3061 ctgtccctcc cgacagccga aacccaaaca caggatttcg gctgttttcg taccaaata
//
LOCUS       DAXHKX010000202         3107 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXHKX010000202 DAXHKX010000000
VERSION     DAXHKX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.202"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..212)
                     /locus_tag="ACFMWZ_001964"
     CDS             complement(<1..212)
                     /locus_tag="ACFMWZ_001964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935862.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQA"
     gene            272..895
                     /locus_tag="ACFMWZ_001965"
     CDS             272..895
                     /locus_tag="ACFMWZ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC5935863.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1006..2388
                     /locus_tag="ACFMWZ_001966"
     CDS             1006..2388
                     /locus_tag="ACFMWZ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC5935864.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEVLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2392..2916
                     /locus_tag="ACFMWZ_001967"
     CDS             2392..2916
                     /locus_tag="ACFMWZ_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC5935865.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          620 a          846 c          939 g          702 t
ORIGIN      
        1 gcctgctgcc gcccgaaaag cgtgatgcgc tgatggtgct gctgcataat ccggacgacg
       61 gataaccggt gaaaacggaa atgcccgggc ggtgttgcct gtgcctgttt ggacaagcgt
      121 tcttgagggc ggtagaattg aggtttgccc gaaaagggca gggcgatatg ccgcgcttgg
      181 attttgcgcg cggtttttcg cgttggaagc aatcattgtc tgtttgaata tttaatgccg
      241 tctgaaacgt ccggacgcgc ccccgttccc tatgaaaaaa ccgaccgata ccctacccgt
      301 taatctgcaa cgccgccgcc tgttgtgtgc cgccggtgcg ttgttgctca gtcctctggc
      361 gcacgccggc gcgcaacgtg aggaaacgct tgccgacgat gtggcttccg tgatgaggag
      421 ttctgtcggc agcgtcaatc cgccgaggct ggtgtttgac aatccgaaag agggcgaacg
      481 ttggttgtct gccatgtcgg cacgtttggc aaggttcgtc cccgaggagg aggagcggcg
      541 caggctgctg gtcaatatcc agtacgaaag cagccgggcc ggtttggata cgcagattgt
      601 gttggggctg attgaggtgg aaagcgcgtt ccgccagtat gcaatcagcg gtgtcggcgc
      661 gcgcggcctg atgcaggtta tgccgttttg gaaaaactac atcggcaaac cggcgcacaa
      721 cctgttcgac atccgcacca acctgcgtta cggctgtacc atcctgcgcc attaccggaa
      781 tcttgaaaaa ggcaacatcg tccgcgcgct tgcccgcttt aacggcagct tgggcagcaa
      841 taaatatccg aacgccgttt tgggcgcgtg gcgcaaccgc tggcagtggc gttgattttg
      901 aacccgcgcc gcaaccgaaa tacggcgaat cctgtataat ccgaaaatct gttcactgga
      961 agttcagacg gcattgcaac tgttgatgct gtctgaaaaa atatgatggc aagagataac
     1021 cgcatccaaa tgtttccgca cgaatggcgc gccagtacga cgctttccgg cgtgtacgcg
     1081 ctgcgtatgc tgggtatgtt cctcgtgctg cccgtattgg cggtgtatgc cgcctcgctg
     1141 cccggcgcgg aaggcaacaa aacgctggtc gggctggcaa tgggcattta cgggctgaca
     1201 caggctctgc tgcaactgcc tttgggcatc gcttccgaca agttcgggcg caagaaaacc
     1261 atttatgtcg ggctgattgt gtttgcagcg ggcagcttcc ttgccgccgc cgccgatacg
     1321 ctgcccatgc tggtcgccgc acgcgccata cagggtgcgg gagcggtcag tgcggcggtt
     1381 accgcgctgc tggcggattt gacgcgcgac ggcgtacgta cccgcgcgat ggcgatgatc
     1441 ggtttgagta tcggtctgac gttttcggtc agcctcgtcg ttgcccctgt gattgccgac
     1501 gcggtgggcg tacgcggctt gttcctgctg accggtattt tgaccgtcgc cagtatcggc
     1561 gtggtggcgt ggatgactcc cgacccggag gtttccaagc tgcacgaaga tacgcaggcg
     1621 cagccttcgc gcataggcga agttttgaaa aaccgtaggc tgctgacgct cgatttcggc
     1681 attttcgccc tgcacgccgc gcaaatggca ttgtttaccg cgctgccttt tgcgatgacg
     1741 cagctcggtt tggaaaaaat ccagcattgg aaagtctatc tgccctcgac cattacgggc
     1801 ttggtggtga tggttccgct gattatcgtc ggcgagacgc gcaacaagct caaacaggtt
     1861 tttatcttgg gcatcgcctg catcgcggcg gcacagctcg gtttgctgtc cggtatgcgc
     1921 tcggtaggct tgattaccgc ttatttggtt gtttacttta tcggttttaa tgtgttggaa
     1981 gcgagcctgc cgtctatggt ttccaaaatc gcgccgtccg acctgaaggg tacggcgatg
     2041 ggcgtgtaca acacgatgca gtcgctcgga ctgtttgccg gcggcgcggc aggcggtttg
     2101 ctgtttcaaa aatacggctt ttccggcgtg tttgcctttt gcagcatatt gatgctgctg
     2161 tggctggtga ttgccgtttt atcgcctgcg cccaagcccg tcaaaaacct cagttaccct
     2221 gtcggcggcg tgtggcaggt caatcaggaa gtgttatacc gcgccttgtc ggagcttgag
     2281 ggtgtggaag acatcggttt cagtttcgac gggcagaccg tctatctcaa agtgttgcag
     2341 aagggtttcg atcaggctgc cgctgaaaaa atcatcacag gagtttaaaa aatgtcattg
     2401 aacaaagtca tcctcatcgg ccgcctcgga cgcgatcccg aagtgcgcta tatgcccaac
     2461 ggcgaggcgg tttgtaattt cagcgtcgcc accagcgaaa cttggaacga ccgcaacggc
     2521 caacgtgtag agcgtactga gtggcacaac atcaccatgt accgcaaact ggcggaaatt
     2581 gccgggcaat acctcaaaaa aggcgggctg gtttatctgg aaggcagaat ccaaagccgc
     2641 aaataccaag gcaaagacgg catcgaacgc accgcttacg atattgtcgc caacgaaatg
     2701 aaaatgttgg gcgggcgcaa tgaaaacagc ggcggtgcgc cttacgagga aggttacggt
     2761 cagagtcagg aggcttacca acgccccgcg cagcaaagcc ggcagcccgc ctccgacgcg
     2821 ccgtcccatc cccaagaagc accagccgcg ccgcgccgcc aacccgtgcc tgccgccgcc
     2881 ccggtcgagg acattgacga cgatatcccg ttctgaattt tacggccgga catcctcgcg
     2941 gagggaaatc ataaaggacg gagaaacctt aaaccttacg gggcagggtt tctctttttt
     3001 gcatttgggc cggcttgttg ccgccctttg cgttgggcgg aacgttgcga ttgcgccgtt
     3061 cgggcgtttt gaggcagtga tgcctgccgc acgtccccgc tgttttc
//
LOCUS       DAXHKX010000203         3084 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXHKX010000203 DAXHKX010000000
VERSION     DAXHKX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.203"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            115..2880
                     /gene="tdfH"
                     /locus_tag="ACFMWZ_001968"
     CDS             115..2880
                     /gene="tdfH"
                     /locus_tag="ACFMWZ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC5935866.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYSWYKKYENPQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
BASE COUNT          868 a          770 c          749 g          697 t
ORIGIN      
        1 ccaattgaaa tcttcagacg gtatatcaaa tttacacttt tttaatgttt atgccgcctg
       61 aaaaaaatgc tagtatattt cctaattgtc tgactgttta ttgttgagga aaatatgaga
      121 tcttctttcc ggttgaagcc gatttgtttt taccttatgg gtgttatgct atatcatcat
      181 agttatgccg aagatgcagg gcgcgcgggc agcgaggcgc agatacaggt tttggaagat
      241 gtgcacgtca aggcgaagcg cgtaccgaaa gacaaaaaag tgtttaccga tgcgcgtgcc
      301 gtatcgaccc gtcaggatat attcaaatcc agcgaaaacc tcgacaacat cgtacgcagc
      361 atacccggtg cgtttacaca gcaagataaa agctcgggca ttgtgtcttt gaatattcgc
      421 ggcgacagcg ggttcgggcg ggtcaatacg atggtggacg gcatcacgca gaccttttat
      481 tcgacttcta ccgatgcggg cagggcaggc ggttcatctc aattcggtgc atctgtcgac
      541 agcaatttta ttgccggact ggatgtcgtc aaaggcagct tcagcggctc ggcaggcatc
      601 aacagccttg ccggttcggc gaatctgcgg actttaggcg tggatgacgt cgttcagggc
      661 aataatacct acggcctgct gctaaaaggt ctgaccggca ccaattcaac caaaggtaat
      721 gcgatggcgg cgataggtgc gcgcaaatgg ctggaaagcg gagcatctgt cggtgtgctt
      781 tacgggcaca gcaggcgcag cgtggcgcaa aattaccgcg tgggcggcgg cgggcagcac
      841 atcggaaatt ttggcgcgga atatctggaa cggcgcaagc agcgatattt tgtacaagag
      901 ggtgctttga aattcaattc cgacagcgga aaatgggagc gggatttaca aaggcaacag
      961 tggaaatact cgtggtataa aaaatacgaa aacccccaag aactgcaaaa atacatcgaa
     1021 gagcatgaca aaagctggcg ggaaaacctg gcgccgcaat acgacatcac ccccatcgat
     1081 ccgtccagcc tgaagcagca gtcggcaggc aatctgttta aattggaata cgacggcgta
     1141 ttcaataaat acacggcgca atttcgcgat ttaaacacca aaatcggcag ccgcaaaatc
     1201 atcaaccgca attatcaatt caattacggt ttatctttaa actcatatgc caacctcaat
     1261 ctgaccgcag cctacaattc gggcaggcag aaatatccga aagggtcgaa gtttacaggc
     1321 tgggggcttt taaaagattt tgaaacctac aacaacgcaa aaatcctcga cctcaacaac
     1381 accgccacct tccggctgcc ccgcgaaacc gagttgcaaa ccactttggg cttcaattat
     1441 ttccacaacg aatacggcaa aaaccgcttt cctgaagaat tggggctatt tttcgacggt
     1501 ccggatcagg acaacgggct ttattcctat ttagggcggt ttaagggcga taaagggctg
     1561 ctgccccaaa aatcaaccat cgtccaaccg gccggcagcc aatatttcaa cacgttctac
     1621 ttcgatgccg cgctcaaaaa agacatttac cgcttaaact acagcaccaa taccgtcggc
     1681 taccgtttcg gcggcgaata tacgggctat tacagctcgg aaggcgaatt taagcgggca
     1741 ttcggagaaa actcgccgac atacagggaa cattgcaacc cgagctgcgg gctttatgaa
     1801 cccgtattga aaaaatacgg caaaaagcgc gccaacaacc attcggtcag cattagtgcg
     1861 gacttcggcg attatttcat gccgttcgcc agctattcgc gcacacaccg tatgcccaac
     1921 atccaagaaa tgtatttttc ccaaatcggc gactccggcg ttcacaccgc cttaaaacca
     1981 gagcgcgcaa acacttggca atttggcttc aatacctata aaaaaggatt gttaaaacaa
     2041 gatgatacat taggattaaa actggtcggc taccgcagcc gcatcgacaa ctacatccac
     2101 aacgtttacg ggaaatggtg ggatttgaac gggaatattc cgagctgggt cagcagcacc
     2161 gggcttgcct acaccatcca acaccgcaat ttcaaagaca aagtacacaa acacggtttt
     2221 gagttggagc tgaattacga ttatgggcgt tttttcacca acctttctta cgcctatcaa
     2281 aaaagcacgc aaccgaccaa cttcagcgat gcgagcgaat cgcccaacaa tgcgtccaaa
     2341 gaagaccaac tcaaacaagg ttatgggttg agcagggttt ccgccctgcc gcgagattac
     2401 ggacgtttgg aagtcggtac gcgctggttg ggcaacaaac tgactttggg cggcgcgatg
     2461 cgctatttcg gcaagagcat ccgcgcgacg gctgaagaac gctatatcga cggcaccaac
     2521 gggggaaata ccagcaatgt ccggcaactg ggcaagcgtt ccatcaaaca aaccgaaacc
     2581 cttgcccgcc agcctttgat ttttgatttt tacgccgctt acgagccgaa gaaaaacctt
     2641 attttccgcg ccgaagtcaa aaatctgttc gacaggcgtt atatcgatcc gctcgatgcg
     2701 ggcaatgatg cggcaacgca gcgttattac agctcgttcg acccgaaaga caaggacgaa
     2761 gacgtaacgt gtaatgctga tggcttgtgc aacggcaaat acggcggcac aagcaaaagc
     2821 gtattgacca attttgcacg cggacgcacc tttttgataa cgatgagcta caagttttaa
     2881 aggcagcccg cgttttgtgg aaaaccgcaa tgtcgtctga aagcccttca gacggcattt
     2941 gtttccccaa acgcatcatc ctgccgcaag cctatgccaa tccgttttat cgcatcggca
     3001 actcaaagaa aaatccattt cattcccacg cagggaagcc ggtttttgat ttcggttatt
     3061 tttggttgtt tcgggtaatt tatg
//
LOCUS       DAXHKX010000204         3079 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXHKX010000204 DAXHKX010000000
VERSION     DAXHKX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.204"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            28..1821
                     /gene="lepA"
                     /locus_tag="ACFMWZ_001969"
     CDS             28..1821
                     /gene="lepA"
                     /locus_tag="ACFMWZ_001969"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC5935867.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            1964..2983
                     /gene="lepB"
                     /locus_tag="ACFMWZ_001970"
     CDS             1964..2983
                     /gene="lepB"
                     /locus_tag="ACFMWZ_001970"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC5935868.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
BASE COUNT          774 a          783 c          799 g          723 t
ORIGIN      
        1 ctaccgttca gcattaaaaa caatccaatg aaaaatatcc gaaatttctc catcattgcc
       61 catatcgacc acggcaaatc gacgcttgcc gaccgcttca tccaatattg cggcggcttg
      121 gatttgcgcg aaatgagtac gcaggtgctc gattccatgg acatcgaaaa agagcgcggc
      181 attaccatca aagcgcaaac cgccgcgctc aactacaaag cacgcgatgg gcaggtgtat
      241 caactcaacc tgattgacac gccgggacac gtcgacttct cttacgaagt ttcccgttcg
      301 ctgtccgcct gcgaaggcgc gcttttggtc gttgacgcgt cgcagggcgt ggaagcgcaa
      361 accgtggcga actgttacac cgcgattgat ttgggcgtgg aggttgtgcc tgttttgaac
      421 aaaatcgacc tgcccgccgc cgaccccgaa cgcgtggagc aggaaattga agacatcatc
      481 ggcatcgatg cggtcggcgc ggtgcagtgt tccgccaaaa gcggcatcgg cgtggaagac
      541 gttttggaag aaatcgttgc caaaattcct gcgccgaccg gcgatgaaaa cgcgccgctg
      601 caagccgtga ttgtcgattc gtggtttgac aactacgtcg gcgtggtcat gctgattcga
      661 gtgaaaaacg gcaccatcaa actgaaagac aaagtgcgct ttatgagcac caaggcggaa
      721 acgcaggtcg agcagctggg cgtattcaca ccaaaatcag ttcaaaaaca agaactcaaa
      781 gccggcgaag tgggcttttt gattaccggc gtaaaagaat tgggtcaggc gaaagttggc
      841 gatacggtta cactggttgc caaccctgcc actgagccgc tgcccggctt ccaagaagta
      901 caaagccagg tattcgccgg actttatccc gtggaaagcc acgactacga agccttgcgc
      961 gacgctttgg aaaaattgca gcttaacgac gcttcgttga aattcgagcc tgaagtttcc
     1021 caggcattgg gcttcggctt ccgttgcggc ttcttgggtc tgctgcactt ggaaatcgtg
     1081 caggaacgtt tggaacgcga gttcgacatg gatttgatta ccaccgcgcc gacggtggtt
     1141 tatgaagtcg tgttgaaaaa cggtgagaaa atcgaagtcg aaaacccatc caaactgccc
     1201 gacatcggca gcatcgaaac cattctcgag ccgattatta ccgcgaccat cctcgtgcct
     1261 caggaatatg tcggcaacgt catgactttg tgtaaccaaa agcgcggcgt tcaggtcaat
     1321 atgcagtaca tgggccgtca agtcatgctg acttatgatt tgccgatgaa cgaagttgta
     1381 atggatttct tcgacaagct taaatcgact tcgcgcggct atgcttcgtt ggattatcat
     1441 ttcaaagagt tccaaccgtc tgatttgatt aagctggata ttatggtcaa cggcgaaaaa
     1501 gtcgatgcct taagcctgat tgtgcaccgt caaagcgcgg ttcaccgagg ccgcgagctg
     1561 gcatcaaaaa tgcgcgagct gattccgcgc caaatgttcg acatcgccgt ccaagccgcc
     1621 atcggcagcc agattatcgc ccgcgaaaac gtcaaagccc tgcgtaaaaa cgttttggcg
     1681 aaatgttacg gcggcgatat tacgcgtaag aaaaaactgc ttgaaaaaca aaaggcaggt
     1741 aaacgccgta tgaaacaagt gggcaatgtg gaaattccgc aaagcgcgtt cttggcgatt
     1801 ttgcaggtga gcgacaaata actctggcac acagggcggc gcgtcgggaa cacccctcaa
     1861 accgccctga tttcaggggc acgcgcccgc aaacaaattt tgcaatttgc cgccttaaaa
     1921 cggttgcacg gacaggcggg tttttaagca ttaaggaaca acaatgaaca caatgctaat
     1981 gtcgggcgcg gctgccgcgc tgcttgccgg catcatcctt tatttcaaaa gcgacaaaaa
     2041 gcggcaggaa aacggtgaat ggagttccgg tctggaatac gcctatatcc tgacggcggt
     2101 cggcgtgttt gccgctttgt ccctgtttat gagctttacc gccgttttcc tgattttcgt
     2161 tgtattgtgc ggtacggctt ggggggtata caaataccgc ctgaagactc atcccgaaat
     2221 ctcggaaagc agccacttcg gcgattattt cggcagtttc ttccctaccg ttttggtatt
     2281 gttcctcatc cggtcgttta ttgccgaacc gttccaaatc ccgtccagct cgatgcgccc
     2341 gggcctgatt aagggcgatt tcattttggt cggcaaattt tcctacggcc tgcgcgtacc
     2401 cgttttaaac aatatattta ttcctacagg caaaatcgaa cggggcgatg tcgttgtttt
     2461 taattatcct ctgcagccgg agatgaccta catcaagcgt attgtcggca ttccgggcga
     2521 tgtggtcgaa tatcgggata agattttgac ggtaaatggc aaacccactt ccgatattcc
     2581 taacggcaca taccgttatc ccgacgatac cgacccttcc gaaatccaca acacggatat
     2641 gttccgcagc ggtttggacg gcaaatcctt caatattctg aaaaaagaag gacagccggc
     2701 cgtttccctg cccgtattgg gcaaatatac ctccgatatt atgtctgaaa acggatattc
     2761 catagagcaa agcggtttgg aacactgcca atatgccgac gacggcagcg gtttcgtgtg
     2821 caaagttccc gaaggacgct atttcgctat gggcgacaac cgcgacaaca gtgccgattc
     2881 gcgctactgg ggatttgtgg atgacaagct ggttgtcggc aaggcaatgt tcattttgat
     2941 gaacttcggc gatttcggca gggccggtac ggcaatccgt taggccagga agccaagaat
     3001 gccgtctgaa aatagtttca gacggcattt gctattcctc acttttgcag gcggatctaa
     3061 cgttgtcgga ttttcctac
//
LOCUS       DAXHKX010000205         3055 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXHKX010000205 DAXHKX010000000
VERSION     DAXHKX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.205"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            822..2993
                     /gene="fetA"
                     /locus_tag="ACFMWZ_001971"
                     /gene_synonym="frpB"
     CDS             822..2993
                     /gene="fetA"
                     /locus_tag="ACFMWZ_001971"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC5935869.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKEYEAGKGFRNFNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDASSRTNITR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVVGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFEISGKKKDPKDPKKE
                     IDKTDEEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHPKKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          813 a          872 c          696 g          674 t
ORIGIN      
        1 gacagggttg ctgttatagt ggatgaacaa aaaccagtac gtcgttgcct cgcctcagct
       61 caaagagaac gattctctaa ggtgctgaag caccgagtga atcggttccg cactatccgt
      121 actgtctgcg gcttcgccgc cttgtcctga tttccgttcg ttttcggtta ttcccgataa
      181 attaccgccg tttctcgtca tttctttaac ccttcgtcat tcccgcgcag gcgggaatct
      241 agttttttaa gttccagttg tttctgataa attcttgcag ctttgagttc ctagattccc
      301 gctttcgcgg gaaggacgcg gaaaggttgc ttttcgtcat tcccgcgcag gcttcgtcat
      361 tcccgcgcag gcttcgtcat tcccgcgcag gcgggaatct aggttcgttc ggtttcggtt
      421 ttttccgata gattcctgcc gcgttagggg ttctagattc ccgctttcgc gggaaggacg
      481 cggaaaggtt gcttttcgtc attcccgcat ttgttaatcc actatatttc cgccgttttt
      541 tatagtggac taacaaaaac cagtacagcg ttgcctcgcc ttgccgtact atctgtactg
      601 tctgcggctt cgtcgcctta tcctgatttt tgttagtcca ctatacattt ccgacaaaac
      661 ctgtcaacaa aaaacaacac ttcgcaaata aaaacgataa tcagctttac acaaatcccc
      721 cctctcgtta atataaacaa aaataattat tatttttctt atcctgccaa accttaacgg
      781 tttggattta cttcccttca tacactcaaa aggacgaaca aatgaacgcc ccgtttttcc
      841 gcctcagcct gctctcgctt accctggcgg caggttttgc ccatgcggca gaaaataatg
      901 ccaatgtcgc attggatacc gttaccgtaa aaggcgaccg ccaaggcagc aaaatccgta
      961 ccaacatcgt tacgcttcaa caaaaagacg aaagcaccgc aaccgatatg cgcgaactct
     1021 taaaagaaga gccgtccatc gatttcggcg gcggcaacgg cacgtcccaa ttcctgacgc
     1081 tgcgcggtat gggtcagaac tctgtcgaca tcaaggtgga caacgcctat tccgacagcc
     1141 aaatccttta ccaccaaggc agatttattg tcgatcccgc tttggttaaa gtcgtttccg
     1201 tacaaaaagg cgcgggttct gcctctgccg gtatcggcgc aaccaacggc gcgattatcg
     1261 ccaaaaccgt cgatgcccaa gacctgctca aaggcttgga taaaaactgg ggcgtgcgcc
     1321 tcaacagcgg ctttgccagc aacgaaggcg taagctacgg cgccagcgta ttcggaaaag
     1381 agggcaactt cgacggtttg ttctcttaca accgcaacga tgaaaaagaa tatgaagccg
     1441 gcaaaggctt ccgtaatttc aacggcggca aaaccgtacc gtacagcgca ctggacaaac
     1501 gcagctacct ggccaaaatc ggaacaacct tcggcgacgg cgaccaccgc atcgtattga
     1561 gccacatgaa agaccagcac cgaggcatcc gtaccgtccg tgaagaattt accgttggcg
     1621 acgcaagttc acggacaaat attacacgcc aagccccatc ctaccgcgaa accacacaat
     1681 ccaacaccaa cttggcgtac accggcaaag atttgggctt tgtcgaaaaa ctggatgcca
     1741 acgcctatgt gttggaaaag aaacgctatt ccgccgatga caaagataac ggctacgcag
     1801 gcaatgtggt cggccctaac cacacccgaa tcaccactcg gggtatgaac ttcaactttg
     1861 acagccgcct tgccgaacaa accctgttga aatacggtat caactaccgc catcaggaaa
     1921 tcaaaccgca agcgtttttg aacggcgaat ttgagatctc cggtaagaag aaagatccga
     1981 aagatcccaa aaaagaaata gataagaccg atgaagaaaa agcgaaagac aagaaagata
     2041 tggatcttgt ccattcctac aaactgtcca acccgaccaa aaccgatacc ggcgcgtata
     2101 tcgaagccat tcacgagatt gacggcttta ccctgaccgg cgggctgcgt tacgaccgct
     2161 tcaaggtgaa aacccacgac ggcaaaaccg tttcaagcag caaccttaac ccgagtttcg
     2221 gcgtgatttg gcagccgcac gaacactgga gcttcagcgc aagccacaac tacgccagcc
     2281 gcagcccgcg cctgtatgac gcgctgcaaa cccacggcaa acgcggcatc atctcgattg
     2341 ccgacggcac caaagccgaa cgcgcgcgca ataccgaaat cggcttcaac tacaacgacg
     2401 gcacgtttgc cgccaacggc agctacttct ggcagaccat caaagacgcg cttgccaatc
     2461 cgcaaaaccg ccacgtctct gccgccgtcc gtgaagccgt caatgccggt tacatcaaaa
     2521 accacggtta cgaattgggc gcgtcctacc gcaccggcgg cctgactgcc aaagtcggcg
     2581 taagccacag caaaccgcgc ttttacgata cccatcctaa aaaactgttg agcgcgaatc
     2641 ctgaatttgg cgcacaagtc ggccgcactt ggacggcttc ccttgcctac cgcttccaaa
     2701 acccgaatct ggaaatcggc tggcgcggcc gttatgttca aaaagccgtg ggttcgatat
     2761 tggtggcagg tcaaaaagac cgcaacggca aattggaaaa cgttgtacgc aaaggtttcg
     2821 gtgtgaacga tgtcttcgcc aactggaaac cgctgggcaa agacacgctc aatgttaatc
     2881 tttcggttaa caacgtgttc aacacgttct actatccgca cagccaacgc tggaccaata
     2941 ccctgccggg cgtgggccgc gatgtacgct tgggcgtgaa ctacaagttc taaaacgcac
     3001 atcccgaaaa aatgccgtct gaaagccttt cagacggcat ctgttctgat aattt
//
LOCUS       DAXHKX010000206         2969 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXHKX010000206 DAXHKX010000000
VERSION     DAXHKX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.206"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            124..1668
                     /gene="purF"
                     /locus_tag="ACFMWZ_001972"
     CDS             124..1668
                     /gene="purF"
                     /locus_tag="ACFMWZ_001972"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC5935870.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1768..2259
                     /gene="greB"
                     /locus_tag="ACFMWZ_001973"
     CDS             1768..2259
                     /gene="greB"
                     /locus_tag="ACFMWZ_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC5935871.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2318..2944
                     /locus_tag="ACFMWZ_001974"
     CDS             2318..2944
                     /locus_tag="ACFMWZ_001974"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC5935872.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVDEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLFR"
BASE COUNT          711 a          907 c          773 g          578 t
ORIGIN      
        1 tcttattctc cataaaccca cctatctgtc cttcgctcaa atattgtcaa caattcaggc
       61 aaaaaaccac cgtttacttt ataattccgt tttaccctta aacctcgaat cggagaagac
      121 gatatgtgcg gcgtattagg tttggtcagt catgaacccg tgaaccagct tctgtacgac
      181 ggcttgcaga tgttgcagca cagggggcag gatgcggcgg gcattgcaac ggcggaaggc
      241 ggtaccttcc atatgcacaa aggcaaaggc atggtgcgcg aagtgttccg cacacgcaat
      301 atgcgcgatt tgaccggcaa cgccggcatc gcccacgtcc gttatcctac tgcgggcaac
      361 gcaggcagca gcgcggaggc gcagcctttc tacgtcagct cgccgttcgg catcgttttg
      421 gcgcacaacg gcaacctcac caacactgcc gaactgtatg aaaacgtgtg taacaaacac
      481 ctgcgccacg tcaacaccag ctccgattcc gaagtcttgc tcaacgtatt cgcgcacgaa
      541 ttacgccgcg aagtctctaa aaacgccgac ccgcaccggc tcaatgccga caatattttc
      601 aacgccgttg cccaagtcca ccgcctggtg cgcggcgcat acggtgtgat tgccatgatt
      661 gcgggctacg gtatgctcgc cttccgcgac ccttacggca tccgcccgct ggtattgggt
      721 tcgcaaaccg acagcgaagg cagaaaatcc tatgccgtcg cctccgaatc cgtcgccttc
      781 aacgcgctta cctacgattt ggaacgcgac atccggccgg gcgaagcggt atttgtcggc
      841 tttgacggca caatgattgc ccgccaatgc agcgaccgtg ccaaactcag cccctgcctt
      901 tttgaatatg tttattttgc ccgccccgac tctgtgattg acggcgtatc cgtttaccaa
      961 tcgcgcttgg atatgggcgt gtcgctggcg gaaaaaatca aacgcgagct gcccgtggac
     1021 ggcatcgatg tcgtgatgcc catccccgac accagccgcc ccagcgcgat ggagcttgct
     1081 gtccacctca aaaagcctta ccgcgaaggt ttgattaaaa accgctatat cggccgcacc
     1141 tttattatgc ccggccaggc gacgcgcaaa aaatccgtgc gccaaaaact cagcccgatg
     1201 gaaaccgaat ttgcaggcaa aagcgtgttg ctggtggacg actccatcgt gcgcggtacg
     1261 accagccgtg aaatcgtcga aatggtacgc gcggctggcg cgcgcaaagt ctatatcgcc
     1321 tccgccgcgc ccgaagtgcg ctatcccaac gtatacggca tcgatatgcc cacgcgcgaa
     1381 gagttgattg ccaatgggcg cagcgcggcg gaaatcgccg ccgaaatcgg cgcggacggc
     1441 atcgtgtttc aagacttggg cgatttggaa gccgtcgtca aagcactcaa cccgaaaatc
     1501 gaatccttcg attcgtcctg tttcaacggc atttatcaga ccggcgacat cgatgatgcc
     1561 tacctcgacc gcctgtctgc cgagaaatcc ggctgcggcg gtctgaaaat ccacccgagc
     1621 aggatggaac acagcatcag catcagcgat gcgggcgacg aagaataagg cttcccaaac
     1681 cgcgatgccg tctgaaatcc gaacgccggg ttttcagacg gcatttccca tatcggcatc
     1741 acatttcccg tttcatccgc acacaatatg agtaccgaaa ccaaaaacta cattacgccc
     1801 gccggatggc aggcattgaa agacgagctt tatcaactgg tcaacaaaga acgccccgaa
     1861 atcgtccaaa tcgtcaactg ggcggcaggc aacggcgacc gcagcgaaaa cggcgactat
     1921 ctttacggca aacgccgtat gcgcgaaatc gaccgccgca tccgtttcct gaccaagcgt
     1981 ttggagtccg ccgtcgtcgt cgatcccgaa ttgcgcgaag cgaccgacca agtatttttc
     2041 ggcgcgaccg tcggcctgtt gcgcggcgac gggcgcgagc aaaccgtcaa aatcgtcggc
     2101 atcgatgaaa tcgataccgc gcaaaacaaa atttcttgga tttcgccgtt ggcgcgttgc
     2161 ctgattaaag cgcgcgaagg cgacgaagtg gttttgaaca cgccggaagg gcgtgaggaa
     2221 attgaaatcc tttctgtcga atacatcaaa atcgactgat cgggaatgcc gtctgaaacg
     2281 gtgcaagcgc gccgtttccc aaataaagga ataccgaatg aggaaaatcc gcaccaaaat
     2341 ctgcggcatc accacaccgg aagacgcatc ggccgccgta gcggcaggtg cagatgccgt
     2401 cgggctggtc tttttccaag gcagcagccg ggccgtcgat attgcccgcg ccaaaaaaat
     2461 caccgccgca ctgccgccgt ttgtcagcgt tgtcgccctt ttcgtcaacg aaagcgcgca
     2521 aaacatccgc cgcatccttg ccgaagtgcc gatacacatc atccaattcc acggcgacga
     2581 agacgacgca ttctgccgcc agttccaccg cccctatatc aaggccattc gtgttcagac
     2641 ggcatcagac atccgaaacg ccgccgaccg cttccccgac gctcaggcac tgctgttcga
     2701 tgcctatcac ccttcggaat acggcggcac cggacaccgc ttcgactgga cgctgttggc
     2761 ggaatattcg ggcaaaccgt gggtgcttgc cggcgggctg acccctgaaa acgtcgacga
     2821 agccgtccgc atcaccggag cggaagcggt cgatgtatcc ggcggcgtgg aagcgtctaa
     2881 aggcaaaaaa gacccagcca aagttgccgc ctttatcgca accgccaacc gcctattccg
     2941 ttaaagcaac aaaaattgcc gctggaatg
//
LOCUS       DAXHKX010000207         2962 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXHKX010000207 DAXHKX010000000
VERSION     DAXHKX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.207"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..460
                     /locus_tag="ACFMWZ_001975"
     CDS             <1..460
                     /locus_tag="ACFMWZ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC5935873.1"
                     /translation="YISTINVDIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIK
                     PKDKVVLRYQAYPYQKFGHATGEIISVARTALGKQKLSGLGIIFTNPTLLNEPAYLVK
                     VKLEKQTIKAYGENKPLQIGMILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            463..2550
                     /locus_tag="ACFMWZ_001976"
     CDS             463..2550
                     /locus_tag="ACFMWZ_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC5935874.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSITLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2632..2934)
                     /locus_tag="ACFMWZ_001977"
     CDS             complement(2632..2934)
                     /locus_tag="ACFMWZ_001977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935875.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
BASE COUNT          966 a          520 c          499 g          977 t
ORIGIN      
        1 ttatatatca acaattaatg ttgatatagg gcaacaagtt gaaccgtcta aattgctgtt
       61 aagcattgtc cctgaacaaa ctgaattggt cgccaatctt tacataccca gtaaagctgt
      121 tggttttatt aaaccgaaag ataaagttgt tttacgttac caagcgtacc cttaccaaaa
      181 atttggacat gccacaggag aaattatttc agttgccaga actgctctcg gtaaacaaaa
      241 gctatcaggt ttaggtatca ttttcactaa cccaacctta ttaaatgaac ctgcctatct
      301 tgtgaaagtt aaattggaaa aacaaacgat taaagcatac ggagaaaaca agccgcttca
      361 aattggcatg attttagaag cagatattct ccatgaacga aaaaaattgt acgaatgggt
      421 acttgaccca ctttacagca tttcaggaaa aatcaattaa aaatggatta tttatcaaga
      481 ctgtcctttg gatttaacaa aaagctacct gtcattctgc aaacagaagt tgctgaatgt
      541 ggtttagcat gcctgacatc catcttgtcc tattatggct ttcacactga tttaagaacg
      601 ttacgccaaa aatacaccct gtcattaaag ggcgcaaatc ttgcagacat catgagattt
      661 ggcaatgaaa tgaatttaac gccacgagct ttgcgtttag agttagatga gctgtcaaat
      721 ttacaactac cctgcattct ccattggaac ttaaaccatt ttgttgtact ttgttccatt
      781 tccaaagaca gtatcgtcat tatggaccct gctgtcggta tgcgaaaaat caaaatggac
      841 gaagtttcac aaaaattcac agggattgcc ctagaattat tccccaatac ccattttgaa
      901 gagaaaaaag aaacaaagaa aatcaaaata ttatctctat taagggggat ttcaggctta
      961 aaacgctctt taattcaaat gcttatatta gctatttctt tggaagtctt tgcattggtt
     1021 agtccattct ttatgcaatg ggtaatagac catgtcattg taactgctga taaaaattta
     1081 ttactgaccc ttactttggg atttggttta ctggctatcc tgcaacagtt aattagcctg
     1141 ttacaagcat gggtaggtat gcacctatct ataactctta atttacaatg gaaagccaat
     1201 atatttaaaa ggttacttga cttacctaat gactatttca gtaaacgaca tttaggagat
     1261 gtgatttcaa gatttggttc aatagatcat atccaagaaa cactaacttc tacttttttt
     1321 gttttagttt taaatagctt aatggctatt tttactttcg tgttaatgac aatttacagc
     1381 actcaattat cgctgattgt tcttttaaca cttgttttgt acatactaat tcgttggctt
     1441 gcatattacc cattaagaaa tgcaacagaa gaaaatattg ttcatgaagc caaacaaaac
     1501 tcatatttca tggaaaccat tcgtggtatc caatcagtta aattatttga taaacattat
     1561 caaagacatg gcacttggat gagcctattt gtgaatacag tcaataccaa gctgacaaca
     1621 gataaactct ctgctttatt tgaattttca aataaactgt tgtttagcat ggaaaatatt
     1681 atcataattt atcttggtgc aagcgcaatt ttagatggtt catttacagt cggtgttctg
     1741 atggcttttt tggcttataa agggcaattt gaaagcagaa cagcttctct cgttgaccaa
     1801 tacatccaaa tcaaaatgtt agggcttcat gctgaacgtt tggctgacat tactttaaat
     1861 gaaacagaaa ctgaaattat taagtataat catataccta aattagataa tgaacaactg
     1921 gttcttaaag ttgaaaacgt ctcattcaga tatgctgata atgagccata tctttttgaa
     1981 aacattaatt tggaatttaa agataatgaa gcagttgttt taacaggaca atctggtcgg
     2041 gggaagtcca ctttgttaaa cattttaaca ggtagcctaa aacctgaaac tggtacagtt
     2101 agtattaatg ggcatgatat atatcaagtt tctccatcct ttattagggg attgagcggg
     2161 attgttcgcc aagatgatgt cctttttgca ggttctattg gggaaaatat ttcatttttt
     2221 gatgaaagcc caaatatgga gctcattgaa caatgtgcaa aaatggcaca aatacatgac
     2281 gatatactta aaatgccaat gggctatgag accttgattg gcgatatggg aaatatctta
     2341 tcaggtggac aaaagcagag agttatcttg gctcgtgcat tgtataaacg acccaaaatt
     2401 ctatttttag acgaagcaag tagccattta gatgtagaaa atgaacaaaa aattaaccat
     2461 aacctaaaaa gtcttggtat tatgaaaata atggttgcac accgccaaga aacaattcaa
     2521 tcggcagata aaattctgaa tttaggttga acagaacaag acttcatttt tctttaacaa
     2581 aaagtgaagt cttttttcaa ataatttaat agaatacatg aaaatagcgg tttaacgttc
     2641 catttcccaa tcatcacgac tggctttgtg ttttggcgat ttttcagttt cctttttctg
     2701 ttgaatttgt tgttttttct gctcttgttc ccatttttgg gctaatttca cggtctcatt
     2761 ttcagcccat tccatcacgg cacaacgatg tagcttttct ccgatatcgc cattaaagcc
     2821 agctccacga acttcaccat aaattcttga atatttttga ttatattcaa tttcttttcc
     2881 attttcttta aaggatttct cccacttttc acaaacttca tcaaaatctt tcaaagggat
     2941 attttttaag gggctgtcct ag
//
LOCUS       DAXHKX010000208         2924 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXHKX010000208 DAXHKX010000000
VERSION     DAXHKX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.208"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            31..1248
                     /locus_tag="ACFMWZ_001978"
     CDS             31..1248
                     /locus_tag="ACFMWZ_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC5935876.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKLGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTAIAIIGLANIAGCVF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLKMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            1245..1376
                     /locus_tag="ACFMWZ_001979"
     CDS             1245..1376
                     /locus_tag="ACFMWZ_001979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935877.1"
                     /translation="MKECRLKTLRRHFPNTPPVSGLHDTQISLIKPQTTAAIKPPFK"
     gene            complement(1451..1591)
                     /locus_tag="ACFMWZ_001980"
     CDS             complement(1451..1591)
                     /locus_tag="ACFMWZ_001980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935878.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAVSDGI
                     LS"
     gene            1604..2734
                     /locus_tag="ACFMWZ_001981"
     CDS             1604..2734
                     /locus_tag="ACFMWZ_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC5935879.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRLLKEKGIDE
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGDLERLIARDIIRFPGFVNNVSEVIKAH
                     HIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          684 a          865 c          693 g          682 t
ORIGIN      
        1 cgcaaactta accgactcaa aatacacatt atgacgcaca ccgcatcgaa aacacccaaa
       61 ttttggctga ccatcaccgc cgccgcattc atcctgctga ttaccatcgg catgaggatg
      121 acgctcggac tgttcgtcca acccgtcgtc aacaccaccg aattgagcat tgcccaattc
      181 agcctcatca tcgccgtttt ccaactgatg tggggcatat cgcaaccatt gtccggcgcg
      241 cttgccgacc gtttcggcgc gttcagggta ttaagcggcg gcgccgtcct gctcgtctgc
      301 gcctgcctga ttgcccccaa catcccgact tattgggggc tgatgattgc cgtcgggctg
      361 ctgctcgcat tcggcaccgg ttccggcggt ttctccatca ttatggggca ggtcgccgcc
      421 caagtccccg cacacaaact cggcttggca tccggactgg tcaacgccgg cggttcggca
      481 ggacaattcc tgttcgcacc gctggttcaa ggactcgtcg tcctgcccga agtcggctgg
      541 acgggcacat tttacgtttg gggcgcaatc gccctgctga ttctgcctgt ctcatggtgg
      601 cttgccggcg gcaacaacgc cgcccacacc caacacgccc aagccacaca cggacaaagc
      661 ctcggagaag cagtcaaaac cgccttcaaa accccaagct acatcctgct gcacctaagc
      721 ttttttgcct gcggcttcca catcgccttt ctcgtaaccc acctacccac ggaagtcgcc
      781 ctgtgcggac tgcccgccac cgtcgcatcc acagccatcg ccatcatcgg actggcaaac
      841 atcgccggtt gtgtgttctc aggctggtgc acaagccgcg ttcggggcaa atacgtcctg
      901 ttcggactgt acgcctcgcg cgccgcgatg atactcatct acattttttc gcccaagacc
      961 gacctcaact tctacatttt cgccgccgca ctcggattta cctggctcgc caccgtcacg
     1021 ccgaccgcct ccattaccgg caaactcttc ggcacacgct acctcgccac cctgttcgga
     1081 ctgaagatgc tcagccacca aatcggcgga ttcctcggct cgtacatcgg cggcatcgtg
     1141 attacacaat tcggcgacta cggctggatg tggtacgccg acgcactgct ggcgggaacc
     1201 gccgccctcc tcaacctccc catccgcgaa ccccgaaccg ccgcatgaaa gaatgccgtc
     1261 tgaaaaccct cagacggcat tttccaaaca ccccgcccgt ttccggactg cacgacacgc
     1321 aaatcagcct tatcaaaccg caaacaaccg ccgcgattaa accgccattc aaataagtcc
     1381 gttttccctt cgggtgtaaa atcatcacaa aacgcgatac tgtatatcgc cgggcgttat
     1441 gcagcggatt tcaggataaa atgccgtctg aaaccgcccc tgcccgggcg ggcgaagcgt
     1501 cggatgcggt gcgcttggca aaggtttgcc cgccgcgccc cgtatccggc atttcatcaa
     1561 tgcagaccgc atttccaatc ctataattca aggtgtccgc cttatgaaaa tcgtttttat
     1621 cacaacagtc gcatccagca tttacggttt ccgcgccccc gtcattaaaa aattaatcgg
     1681 caaaaaccat caggtgtatg cctttgtatc ggagttttcc gacaatgaat tggatattat
     1741 cagggaaatg ggggttacac ccgttaccta ccgttcaaac cgcagcgggc tgaacccgtt
     1801 ttccgatata aaatccacct tcctcatctt taaagaactc aaaaaaatat cgccggattt
     1861 ggttttccct tatttcgcaa aacccgtgat tttcggcact tttgccgcaa aactggcagg
     1921 cgtgcccaga atcgtcggga tgctggaagg tttgggattc gcatttaccc cgcagccgga
     1981 aggcataccg ttaaaaacaa aaatcataaa ggggattttg attgccttgt accacattgc
     2041 cctgccgatg ttggaaagcc tgattgtatt aaaccccgac gacaaagacg aactgacgga
     2101 caaatacggc atcaaaataa aaaacatcca tattttgggc ggaatcggtc tggatttgcg
     2161 gcaatatcct tattccgagg cggatattcc cgatgaaaaa gaacccgtaa aatttctctt
     2221 tatcggcaga ttgttgaaag aaaaagggat tgatgagttt attcgggcgg cggaacaggt
     2281 taaggacaaa taccccgata cggtttttac cgctttgggc gcaatcgaca aatcacgcgg
     2341 gggggggggc gatttggaac gccttatcgc ccgcgatatt atccgtttcc ccggttttgt
     2401 gaacaatgtt tccgaagtga taaaggcgca tcatatattc gtattgccgt cttattatag
     2461 ggaaggcgtt ccccgaagca ctcaggaggc aatggccgtc ggcagggcag tgattacgac
     2521 ggatgtcccc ggatgcaggg aaacggtcgc cgacaaggtc aacggcttcc tgatcgaacc
     2581 ttggaatccc cgcatcttgg ccgaaaaaat gatttatttt atcgaaaaca gggaagccgt
     2641 ccgcctgatg gggaatgcaa gttatgcgat tgccaaagat aaattcgatg ccgaaaaagt
     2701 cgatttgaaa ttgctcgata ttttgaaggc gtaaacaagg ctgcccgctt ttgagtttcg
     2761 gtcatttctg ataaaccccc gtcattcccg ccacaccccc gtcattccct cgaaagcggg
     2821 aattcaggtt cgtttagttt cggtcatttc cgataaattc ttgcaacttt gcgtttctag
     2881 attcccactt tcgtgggaat gacggcggag ggttgctgtt ttcc
//
LOCUS       DAXHKX010000209         2922 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXHKX010000209 DAXHKX010000000
VERSION     DAXHKX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.209"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(108..1418)
                     /locus_tag="ACFMWZ_001982"
     CDS             complement(108..1418)
                     /locus_tag="ACFMWZ_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC5935880.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTVDGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRALQIVNDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEVLKAK"
     gene            1730..2152
                     /locus_tag="ACFMWZ_001983"
     CDS             1730..2152
                     /locus_tag="ACFMWZ_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935881.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            2840..2922
                     /locus_tag="ACFMWZ_001984"
                     /pseudo
     CDS             2840..2922
                     /locus_tag="ACFMWZ_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          801 a          704 c          705 g          712 t
ORIGIN      
        1 ttgtctttat tggctttatc gataaacagg ataagacctg aaaaaagccg atacgccttt
       61 ttggtgtacc ggctttgcca tactgttctg cttcagacgg catcgcttca ttttgccttt
      121 aatacttctt cgtccagcga tttcaaccac gccagctttt cgccgatttt gatttccagt
      181 ccgcgcggga cgggttggta gaaatcgggt tcgtccaggc cgtcgggcat atagctttcg
      241 ccggcggcgt aggcgttcgg ttcgtcgtgg gcgtagcggt attcgcgtcc gtagcccaat
      301 tccttcatca gcttggtcgg ggcgttgcgc aggtggacgg gcacttcgtc gctggcgttt
      361 tctttgacga agtggcgcat ttggttgtat gccttgtagc ccgcgttgga tttcgcggcg
      421 gcggcaagat acaataccgc ttgcgccaaa gccagttcgc cttcgggcga gcctaagcgt
      481 tcgaaggtgg cggcggcatc gttgacgatt tgcaaggcgc gcgggtcggc aagcccgatg
      541 tcttcccaag cgatacgcac gatgcggcgg gcgaggtagc gcgggtcggt gccgccgtcg
      601 agcatacggc agaaccaata cagcgcggcg ttcggatgcg aaccgcgcac ggatttgtgc
      661 agggcggaga tttggttgta gaaactctcg ccgcctttgt cgaaacggcg gatttgcgcc
      721 ccgagactgt cggcgagaaa ttcggcggtt aagtttttca gacgacgtgt atcggcggcg
      781 cgtaaaagtt gttccaacaa attcaacaat ctgcgcgcat caccgtcgac ggtattcacg
      841 agtaattttt gcgcatccgt ttcaatcgta aactcttggt attcaggcaa agccaatacc
      901 ttggcaatca gctttttcag gtcgtctgaa gacaagggtt gcaaaacata cacctgagcg
      961 cggctcaaca gcgcgggatt gacttcaaac gacggatttt ccgtcgtcgc accaataaag
     1021 gttagcaaac cgctttcgac atgcggcaaa aacgcgtcct gctgcgcctt gttgaagcgg
     1081 tggacttcat cgacaaacaa aatcgtcgcg cgtccctgct gcaaagcgat ttcggcttta
     1141 tcgattgcct cgcggatgtc cttcacgccg gaaaatacgg cggaaacagg caaaaactgg
     1201 gcgttgaaac tctgcgccaa aatccgcgcc aacgtcgtct tgcccacgcc cggcggcccc
     1261 cacagcaaca tagaatgcgg cttgccgcct tctaccgcca cgcgcaaagg tttaccttcg
     1321 ccgatgaggt gttcctgccc caccacgtcg tcaagcgtat gcggacgcaa tcgttcggca
     1381 agcggcgcgt cgggttctcg ggcaaacaaa tcggtcataa cggctccgtc aacaggtttt
     1441 caaacaatat gattatacgg cagggaacgg cggcgtgccg catacggatt ccgcccctcc
     1501 gtttgcctta agccgatatt aggcgcatac tggaaaagac gagagacttc acacaatata
     1561 tccggcacgg agaccgattc cgcatcagca tgacaatacc caaatcagcg tttcaattaa
     1621 acattaagga gactaaaata gaaaatttgc tttatctacc attgctttgt tgattcaatc
     1681 ggcattatgt tttgaggcgg aagcccatga atatactaat attcaagaga tggaatgggt
     1741 gtctttattt tctgatccgc aaagagacga tgatagtctt ataaccctta aagatgaaaa
     1801 aatcactgta aaaaactata ttgtgccttg gtggaaaaaa ggtgaaaact ttagaaaatt
     1861 agaacttatt accactggta aaaatgataa tggaaattta acggtaaatg gaatttacgg
     1921 catccaatct tttgcagact ctcaattggg atatacgcct gaattattgg taaaaagcaa
     1981 taatgatatt ctatttaaaa ataatctcca atctgttgac gtgaagcccc attccggaaa
     2041 tatccatttg gatgccaaag gaaaaattca atttaatttg aatgttcccg atgttgatgg
     2101 ggtacaagat gtagccagtg gaaaaaaata ccgacaaaca atagtgcctt agtgcggtat
     2161 gtcaacacta tcagcccccc gatgaaaatg acaaaggcag catatccttt cattcaaaaa
     2221 caggcaattt gttcaatatg aacgttaaag ttcataaaga cacacaccat ttcggtattc
     2281 acaacctcaa caataccgat gttcatctga ctgccgaaga cagcgataat gtgattgaga
     2341 ttaaagggga actcgccgac cttatgccga caggaaatgt tgagagcaca agttttttaa
     2401 ggggtattga aacggctttt aaatccaata ccgtattaac ggggaataac aataaaatat
     2461 ccatcgccac tgagggcaag ggcttgggat tgggcgtaaa cggtctccag tccaactccc
     2521 cctattccgg acacacgacg ggcattttca tcaataatca atcaagcgat cttacccaac
     2581 cttcccttga cggcatcagc accatcaaac tcaaagccgt caaggggaat aatgaagtta
     2641 atttaaatgt aaaacaccac gcctccgtaa aaggcattat cgcgtcgcat tctgcaaaag
     2701 caacgcttga agccgagggg gataatattg tccgggttaa aaatccggac accaatacgg
     2761 ctttaatcga tgctttgaaa aaacaatacc ccaatgcttc gaaagaacta tacagggtgg
     2821 gggttcaatc cacgactgga acagtacgca tttcgccaaa cggcaaaatc gcattaacgg
     2881 aattgaaaac ttttggaatt gtacaaaacg gcatttgcgt ca
//
LOCUS       DAXHKX010000210         2878 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXHKX010000210 DAXHKX010000000
VERSION     DAXHKX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.210"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(114..189)
                     /locus_tag="ACFMWZ_001985"
     tRNA            complement(114..189)
                     /locus_tag="ACFMWZ_001985"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Phe,seq:gaa)
     gene            complement(380..1114)
                     /gene="lptB"
                     /locus_tag="ACFMWZ_001986"
     CDS             complement(380..1114)
                     /gene="lptB"
                     /locus_tag="ACFMWZ_001986"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC5935882.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(1188..1718)
                     /gene="lptA"
                     /locus_tag="ACFMWZ_001987"
     CDS             complement(1188..1718)
                     /gene="lptA"
                     /locus_tag="ACFMWZ_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC5935883.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(1699..2280)
                     /gene="lptC"
                     /locus_tag="ACFMWZ_001988"
     CDS             complement(1699..2280)
                     /gene="lptC"
                     /locus_tag="ACFMWZ_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC5935884.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(2277..2813)
                     /locus_tag="ACFMWZ_001989"
     CDS             complement(2277..2813)
                     /locus_tag="ACFMWZ_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC5935885.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT          622 a          745 c          755 g          756 t
ORIGIN      
        1 cactatacct gcccgacagc cgcaaagacc gtatcataca tagcaggcat tgcggtgcgg
       61 gattactgcc gcaaacggca ggcaaaaaaa ataaccgctt caaggcggtt ttttggtggc
      121 cagaggcgga atcgaaccgc cgacacacgg attttcaatc cgttgctcta ccaactgagc
      181 tatctggcct ttgtctgtct cgttaagaga tgcgtattaa accaaattac gggacgacat
      241 gcaagcatat cgggcaatat tctttgaaaa caggtttaag tcaatgtttt aaatttgaat
      301 taatttatgt aaaaacaaca taaatccgta tttttgccgc ctgcccgacg atattaggtc
      361 gcccgtctga aaaatatttt caatatttga agttcttacc cagataaaca gaacgaacct
      421 gttcgtttcc gaccaaatca tcaggttttc ccgatgccag caccgcaccg tctgaaatga
      481 tgtaggcgcg gtcgcagatg ctgagggttt cgcgtacgtt gtggtcggta atcagtacgc
      541 cgataccgcg cgacttgaga aatccgatga ttttttggat gtcgatgacg gcgatgggat
      601 caacaccggc aaaaggctca tccagcaaaa taaaatgcgg tttcatggcg agtacgcggg
      661 cgatttcgac gcgccgccgt tcgccgccgg acagcgacgg cgcggggctg cggcgcaagt
      721 gtccgatatt aaggtcggcg agcagttttt cgatttccct gtcgatttga tttttatctt
      781 tggttctgat ttccaagatg gcgcggatgt tttgttcgac ggtcattttg cggaatatcg
      841 aggcttcctg cggcaggtag ccgacaccga ggcgggcgcg ttcgtgtatg ggcaggtggc
      901 gcaattcttg tccgtctagg gttacgctgc ctgcgtcggc ggcaatgagt ccgactatca
      961 tgtagaagct ggtggtttta cccgcgccgt tgggcccgag caggccgacg acttctccgc
     1021 tttcgatttc gagggagaag cttttaacga cttggcgttt tttgaaactt ttttgcaggt
     1081 tttgaacaac aaggcggctg acgtttgcgc tcataaatat ctcttcaata tcttcggtcg
     1141 gcaaatgccg tccgaaccag gtttatgttt cagacggcat tttgggatta ttcggatttt
     1201 tgcgtactcg aaggctggat aacgacgctg accctgccgg atttggaagc ggattttgcg
     1261 ccggattttg tgctgccgct gatggtatag acttcggttt tggtgttgta tgtaatcacc
     1321 gcaccttcgg cgacatcgcc gccgcgctgt actttggcat taccggttaa gactacggtg
     1381 ctgcctgcag atgaataagc aacgttgttt gcctgtccgc gcaccgtgcc tttgccgccg
     1441 tccaatgtct ggctgaagcg gactggcgaa ccttccgccc tcacggattc gccgcctttg
     1501 ccgccgcgtg taacattgac gcgggcggcg gaaatattga gcgtaccctg tctgatgacg
     1561 acgtttccgc tgaatgtggt gctttggttg gcttgatcga gcgaaccttg gtcggcctca
     1621 atctgaatag gctgcctgct gtcgctttga agggcaaaag cgggggacgc cgaaaaaaat
     1681 gctattaaaa caaatagctt acatatcttt tgtatcataa atcgtggctt tcactttaga
     1741 tgagaagttc aacatgcctg ttttgtggtc gtaagtcatg ccgcccgcct gaccgtgcga
     1801 tgcaccatat tggaaactga caggcgtatc ggtttgggca tattgagatt cggtatcgac
     1861 gtgcagcttt tcggcttcaa ctttacccgc ctgccgtttg ccgtcggcgg ttttggtcag
     1921 cacaacgttg tttttaaaaa gaacctgttt gttttcggta tggtaaacgg cttcatcgct
     1981 gccgacttcg tacaacaacc tgccttcttg gaagaacacg agatgcggtg agtcgaaatg
     2041 aatgtcgctg ctttcgggaa actgtttcgc gcccttcgaa ctcaaatgtt ctttcaagta
     2101 tccctgttcg tcaaaccgcc tgccatccaa tccgtccatt gtgtattgcg gttcgtcggg
     2161 attgagcctg acttcttcaa tctcgacttc gctgatgcgt cccaaccatg ccgacaggct
     2221 gcccaaggca accgccaata tcaatgggaa cgcaattccg taccgccatc ttactttcat
     2281 ttgatgtact cgttcaaagc cgcgcccaaa gtcccttgcg cctgcataat caggtcgcac
     2341 acttcgcgca ccgcgcccgc accgccgggg cgtttggcaa tataggcggc gtgttgcagc
     2401 gtaaaccaat gcgcgtcggg gacggcaacc ggcaatccgc agcgtaccat aacgggcaaa
     2461 tcgaccacgt cgtcgccgac aaaggcacac tcggcttctt ccacgccgac ctgagcgcgc
     2521 agctcttcat acgcggcgcg tttgtccgaa atgcctttga agtagtaatt tatgcccaac
     2581 tgtttgacgc ggatgccgac ggagggcgcg tcccgacctg tgataatcgc agtctgcacg
     2641 ccgcttgcct gaagcatttt cagaccgtgt ccgtccagcg tgtggaacga tttgatttct
     2701 tcgccgttgt cgcggataaa gatgcgcccg tcggtcaaaa caccgtccac atccaggatc
     2761 agcagtttga ttttggcggc gcgcgcctgt aattcaggag aaattgcctg catgggattt
     2821 ccttgttttc agacggcata tcttaccaca aatgccgtct gaaacggctg caacgcct
//
LOCUS       DAXHKX010000211         2838 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXHKX010000211 DAXHKX010000000
VERSION     DAXHKX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.211"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            81..1423
                     /locus_tag="ACFMWZ_001990"
                     /pseudo
     CDS             81..1423
                     /locus_tag="ACFMWZ_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            1468..1758
                     /locus_tag="ACFMWZ_001991"
     CDS             1468..1758
                     /locus_tag="ACFMWZ_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935886.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1763..1960
                     /locus_tag="ACFMWZ_001992"
     CDS             1763..1960
                     /locus_tag="ACFMWZ_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935887.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            2034..2252
                     /locus_tag="ACFMWZ_001993"
     CDS             2034..2252
                     /locus_tag="ACFMWZ_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC5935888.1"
                     /translation="MKFINTCRKYGAKLAVVTAAPLALAAHANATLPDTAKNALEAAK
                     ADGMEVGWIVVGVFAALFVFSIVKRVMK"
     gene            2259..2537
                     /locus_tag="ACFMWZ_001994"
     CDS             2259..2537
                     /locus_tag="ACFMWZ_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935889.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            2665..>2838
                     /locus_tag="ACFMWZ_001995"
     CDS             2665..>2838
                     /locus_tag="ACFMWZ_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC5935890.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRR"
BASE COUNT          841 a          633 c          658 g          706 t
ORIGIN      
        1 gggacgatta acaaaaatta acgtccttta ctttctacaa gtaacagggc tttttttgcc
       61 cgtttttgag gattcgcacc atggaagata agcaagggat gacaaaggcg gttgccggcg
      121 tgatgacgga cgcgccgacg gacggcagga agccggcaac cgcttcaaac cttcccccct
      181 tatctaacgg gggggtacag aaaccgaaac ggcaggcagg gttcaggaag tcttcgaatg
      241 ttacgaaatg tacataacgg acggtaaagg aaacctgtta ggcgttcctc ttcggcgcgg
      301 tgtatcagat tcggctttca ttgatcaaat tagcttttca tttcatgaaa aaaccttttt
      361 cgataaatac ggcgttcgtg taagtctttt ggaagacgaa gattttattc gcgccgcgtc
      421 catgctcgcc gaagaagttt tcggtttcgg tatctacaaa gaatccaaag gctcgggcgg
      481 tcgtttctat gagcgctgtt ggttgatggg ttcggaagac gtcctatacg gtcgcgtcca
      541 ttttggcgga caaaacaata ccatcctttt cgaactgacc ggcaccggtt gcggcgtcgc
      601 aaaagaaggc tgggaatcac gacttttcac attcctgact aatgcaatcc gcccaaaaat
      661 cacacgcgtt gacatcgcaa aagacttttt caacggcgaa tacagcccga accaagcccg
      721 tgaagaccga aataaaggta tgtttacctg tcatcacgtc aaaccaaaag gcgaatgttt
      781 ggggtcagat tgggaagaag acgatgaagc caaaatgacc aaaggcaaga cctatggtat
      841 cggctcccgt gaatcgtcca aatatgtccg cgtctatgaa aaaggcaagc agttgggcga
      901 taaaacaagc acatggacgc gatttgaaat tgaattcaaa gcaaaagata tcgttatccc
      961 tttcgaagtt ttgcagaatc cgggcgaata tttcggcggc gcatatccga tttgcgaacg
     1021 attcgcccaa aaggcaacgc gcatacacgc ggttaaggaa gataaggtca tttcagccga
     1081 ccgctacctt gaatgggtaa aaaaacagtt cggacgtgcg gcaaacggtc tgaaattcat
     1141 ttttcccgaa ttggacaaag ccaaactgtt tgaactgatt gagccgagtc atcacaagct
     1201 gcccaagtct ttggctcccg aagcctacga ctgcgccttt ttgaaagctc aagccattca
     1261 tgaacagccc gcattcaaac cgtacaaaga cccttactat atgtacgaat attacgagaa
     1321 tcttgaaaaa cagcttgaac agcaaaaaca cgtcaacaat gaagaaagct ataacaactt
     1381 catttacgac aaattcgcaa gactaccgat ttcatgggct taaagtgtct gcccgaaaga
     1441 cgtttaatca cacaaggaaa ccaaaaaatg aacatccaac ttcaaggcca catcgtcggc
     1501 gttaaaaaat tcaacggaca aatcgaaggc aagagcttcg actattgccg cctgattgtc
     1561 gccacaccct tagacagctc ccaaggcaac gcattgggca gctctactac tgaatacgat
     1621 ttcggcggct ctgccaattt cgagcagttc cgaaacgccc aatttcccat cgaagcaaac
     1681 ctgaacgtgg aaatcgttac tacgggcaaa acccaaaaac tgaaagtcat cggttttcaa
     1741 ctcgtgaaga aaggctgatt gaatgcagaa agtctatgtt gtccagtccg tatcaacagg
     1801 ggactttctg tatctctctc ctgaaacggg cgacatcgga cataccaaat taatcaccaa
     1861 tgccgattat ttctacgact tcgaagaagc gattaacgca ggtttggaag aaatcggcaa
     1921 ccaatacgaa tttgtcgtat tcggattttt gaaagactga ttttcggatg ttcggcggtc
     1981 gtttgaaaaa cgctccatcc attaccgcca aacacttttt gaaggaaaat atcatgaaat
     2041 ttattaacac ctgccgtaaa tacggcgcaa aactggctgt tgtaacagct gctcccctgg
     2101 ctttggccgc acatgcaaat gcaacgttgc ccgatacggc aaaaaacgct ttggaagccg
     2161 caaaagccga cggtatggaa gtcggttgga ttgtagtggg cgttttcgcc gcgctttttg
     2221 tattttccat cgttaagaga gtgatgaagt aagacggcat gtactaccaa gtcggaaata
     2281 aatgtcttga gaagcaccag gctgaaaacc tttatttcag cttggtagta ccaagaatca
     2341 aagaaaacgg acagattgtc agaccggaat ataacggcag tctgtggaag atgtcggacg
     2401 gtcagccgct aaggctttta ttggcggaat gcagtccgaa agacaacctg caaagcggtc
     2461 ttgaaacagg ctggatagta ttcggcatcc tcgcgtccgt ttactttgtt tccctgctga
     2521 aaaaggtttt gaaatgatgg atttttattt ttatctcggc gtttccgtac ccgtattaat
     2581 cggggcggtt ctgtttaaga attgagcgca tgaagttatg gtgtcaaaat caggctttca
     2641 aaacaatcat tgaaaggcag aaccatgaac aagccgttta tcactcaggc gcagttggca
     2701 ctttataaat atcagccgtc tagcaagtat tttgggcaat cgatggcggt tatagcgcaa
     2761 tctgaatttg ttgaatttgc gaagattaat aagtctgaaa atgttattga ttgtttctct
     2821 tttttctgga atagaaga
//
LOCUS       DAXHKX010000212         2813 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXHKX010000212 DAXHKX010000000
VERSION     DAXHKX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.212"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..235
                     /locus_tag="ACFMWZ_001996"
     CDS             <1..235
                     /locus_tag="ACFMWZ_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC5935891.1"
                     /translation="KPFINILINHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTS
                     ENPRYAPFEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            445..1059
                     /locus_tag="ACFMWZ_001997"
                     /pseudo
     CDS             445..1059
                     /locus_tag="ACFMWZ_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1091..1759
                     /gene="hda"
                     /locus_tag="ACFMWZ_001998"
     CDS             1091..1759
                     /gene="hda"
                     /locus_tag="ACFMWZ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC5935892.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            1756..2424
                     /locus_tag="ACFMWZ_001999"
     CDS             1756..2424
                     /locus_tag="ACFMWZ_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC5935893.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT          776 a          840 c          619 g          578 t
ORIGIN      
        1 aaagcctttt ataaacattc taatcaacca cgccgaaacc gagcctcgcg acggcctata
       61 tgtcttgcgt ataggcaatg acctctttgt caaacgtgtg caacgtatac cgggcaaact
      121 gcttgtaacg tctgaaaatc cacgttacgc accgtttgaa atagatttga gtaataccca
      181 agacgacatc gccatcatag gccgtgttga gtggtatggc cggagtattg attaaatcca
      241 ttttaaaaac ctcttaaaac agttttaaaa attccctaat ctcgtcagct ttaaacagaa
      301 aaccgcgcat tccggcgcgg ttttgtgaaa aagctggcgt aacttttccg gatacaaaaa
      361 aacgccgaaa tccacgtctt ccgaagattt cagcgttttt ttactctatt tgttccttgt
      421 gcaaaaacta acagtccccc acaggaaaac gtcgccctgt tccgccacaa cggcgaagtt
      481 ttattggacg gcatcaacat caaactcaaa agcggcgatt cgctgctgat acgcggcccg
      541 agcggttgcg gcaaaacctc gctgctgcgc gcgctggctg ggctttggcc gttcggcagc
      601 agcggcaaag tcagccgtcc gccgcatcaa gacatcctct tcctgccgca acgcccgtac
      661 acggcacagg gcagcctgcg cgacgcggtt tgttatccgg acatcgacaa acagcatccc
      721 gagttagccg aagccatgaa cacctgccgc ttgggttatc tgattgacaa attggacaaa
      781 acagacgact ggcagcacaa actctccccg ggcgaactgc aacgcgtcgc cttcgtccgc
      841 gccctgcttt ccaagcccaa aatcgtcctg ctcgacgaag ccaccgccgc cttggacgaa
      901 ccggccgaag ccgcgctcta ccgcgcctta aagcaaaaac tgcccgacag catcatcatc
      961 agcatcggac accgaagcac gctcaacgcg ttccacaata tgcgcctcga tgtcggcatc
     1021 gttcaaattg gaaaggcgcg cgaaaacaac gtacaataaa cccgtttacc acccctcgga
     1081 atgccgaacc gtgaaccagc ttattttcga ctttgccgca cacgactatc cgagtttcga
     1141 caaattcctc ggcacggaaa acgcggaact ggtctatgtc ctccgacaca aacacggaca
     1201 gtttatctat gtctggggtg aagaaggtgc gggcaaaagc catcttttgc aggcgtgggt
     1261 cgcacaggcg ctcgaagccg gcaaaaacgc cgcctatatc gatgccgcct ccatgccgct
     1321 gaccgatgcc gcgttcgagg cggaatacct cgccgtcgat caagtcgaaa agctgggcaa
     1381 cgaagaacaa gccctgcttt tttccatctt caaccgcttc cgcaacagcg gcaaaggctt
     1441 tttgctgctc ggttcggaat acacgcccca gcagctcgtc atccgcgaag atttgcggac
     1501 gcgtatggcg tactgcctcg tttacgaagt caagccctta accgaccaag aaaaaatcga
     1561 cgcgctcgcc agtatggcgg cggcacgcca agtaaccgtc gattccgaaa ttttcgaata
     1621 cctgctcaaa cactggcggc gcgatatgga cagcctgatg atgatgctcg acacgctgga
     1681 caactacgcc gtcacaatgg gcaaacgcat caccctgccg cttttgcgcc agcttttgaa
     1741 acaacaggaa acccaatgaa aaacctcgcc atcttcgacc tcgacaacac cctcatcaac
     1801 accgattccg accacgcctg gccgcaatac ctcatcaaaa aaggcttggt tgacgctgcc
     1861 gaaaccgaag cgcaaaacga aaaattctac cgcgactacc aaaacggctg cctcgacatc
     1921 gacgcattcc tcaaattcca cctcgccccg ctcgcccgct acagcaaaga agagctggca
     1981 gagtttcacc gcgaatttat ggcggaatac atcatccccc acatctcgcc tatgcagcgt
     2041 atgctggtgc agagccacca aatggcaggc gacgaaaccc ttgtgatttc ctcaaccaac
     2101 gagttcatca tcacccccgt ctgccgcctt ttcggcatta ccaacatcat cggcacacaa
     2161 ctcgaatccg gttccgacgg ccgctacacc ggcaattaca tcggcacgcc cagcctcaaa
     2221 gaaggtaaaa tcacccgcct gaaccaatgg ctcgccgaac gcggcgaaac gctcgaaagc
     2281 tacggcaaaa cctattttta cagcgactcc aaaaacgacc tgccgctgct gcgcctcgtc
     2341 gacgaacccg tcgccgtcaa ccccgatgcc gagctggaaa aagaagccaa agaaaaaggc
     2401 tggccggttt tgaatttcaa atgacgcaat gccgtctgaa gcccgattcg gcgttcagac
     2461 ggcattgagg ctgaaatcca aaaaactcaa agccacagga atttatccgg aaaaacaaaa
     2521 aacacaggaa ttcatcggga aaaacaacaa tctttccacc gtcattccca cgaaagtggg
     2581 aatcttgatc cgtccgcacg gaaacttata tttcgtcatt tgcacccgac ataattcaat
     2641 actttatttt gcaataaatt aacggatact gacaaattag attggattaa ttaccctatt
     2701 atccatattg ggttatcggg ttgctaattg agcagagcgt agcaaaccat aaagtgagtg
     2761 tttccaccca ctctgcattt atagtggatt aacaaaaatc aggacaaggc gac
//
LOCUS       DAXHKX010000213         2804 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXHKX010000213 DAXHKX010000000
VERSION     DAXHKX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.213"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            53..1456
                     /locus_tag="ACFMWZ_002000"
     CDS             53..1456
                     /locus_tag="ACFMWZ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC5935894.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMNADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLISLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALEWAKTSTKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLAAAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTALDEAVHWFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDIYRYGLGMTSDKEKALHYYRQAAEAGSLAAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDNEKACRYLQEAINSGH
                     EQKSFLQELLQQWQNAV"
     gene            1578..2294
                     /gene="trmB"
                     /locus_tag="ACFMWZ_002001"
     CDS             1578..2294
                     /gene="trmB"
                     /locus_tag="ACFMWZ_002001"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC5935895.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            2513..2804
                     /locus_tag="ACFMWZ_002002"
                     /pseudo
     CDS             2513..2804
                     /locus_tag="ACFMWZ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          804 a          839 c          653 g          508 t
ORIGIN      
        1 ctaacttcat aacacaataa cgataattcg acaaaacgga aaacgatctg atatgaacaa
       61 tcccgactta ccctatcggc aggccttgga acgcctgtct caaaaacaat ataactttac
      121 cgaagtccgc cgactgctga cagaagcggc ctcggcaggc catcccgccg ccgcattcga
      181 gttggcaaaa cacctgatga acgcggacag tccctaccaa gaccgcgaac aaggtatgga
      241 aatgctccgc atcgccgccg aacagggaca tccctacgcg cgttacaatc tggcatatat
      301 ccaagaattg gaaggcgcac ccccggaaac cctgatatcg ctttacagac cattagcaga
      361 ggaagggctg cctgaagcac aagtccgcct gatgtacctg ctgtatgcgt cccgacattt
      421 tgaagaagcc ttggaatggg caaaaacaag cacaaaaaac aacaatcccc acgggcaata
      481 cctgctcgcc caatactgcc ggtacggcac gccgccggat tttgaaacgg cgcacctgct
      541 ctaccgcaaa gcggcggcac aaggcttggc ggcggcacac tggcagctcg ggctgcaata
      601 ccgtttcgga caagggacga aagtcgacac ggcacaggcc gtcaatcatt tgcgcgccgc
      661 cgcacaacaa ggatacattc ccgcctacac cccgcttgcc gagctcatcc tacctacggc
      721 tcttgatgaa gccgttcact ggtttcaaca ggcggcacag gaaaatgacc ccgatgccca
      781 tgccgcactt gccgacatct acctgcaagg caagcatccg gaaagaaact acaaacttgc
      841 cctgcatcac gccgaagcag ccgccgccga acgccatccc gaaggtttgc ggatactggg
      901 cgacatctac cgctacggac tgggcatgac ttccgataaa gaaaaagccc tgcattatta
      961 ccggcaagca gccgaagccg gcagccttgc cgcgtatcag aaactcatat ccgacagcgc
     1021 gttaaaccat cccgaccaat atggcggcat taaagattcc gccatcaggc ggcaaagggc
     1081 agaacggctt tatcaaaaag cccaagccct gcattacgga ttacaatgcg cgcccgaata
     1141 tgcagccgca ctcaaactct acacagaagc cgcagaactc ggccacagca aagcccaaac
     1201 caatctgggc agcatgtatt acttcggaca gggtatgacc gccgactaca atgaagcacg
     1261 caaatggttt gaaaaagccg ccgcaaaaaa agacagtatg gcgttctaca acctcgcctg
     1321 catccattac agcggacacg gtgtcgggcc ggacaacgaa aaagcctgcc gctacctgca
     1381 agaagccata aacagcggac acgaacaaaa aagctttctg caagaactgt tgcaacagtg
     1441 gcaaaatgcc gtctgaacag cgttacacct accctgccga aacgaaacag gtataatcgc
     1501 ccctttcctt tcccgccgtc cgaacaggca tttcacattc agacggcatc ctgattgcac
     1561 aagcgcacga aagcattatg acagacaccg ccgaaaacca aacacaaaac aactggcaag
     1621 ccgaacatcc ccgcagcatc cgcagcttcg tcctccgcca aagccatatg accgccgcgc
     1681 agcaacgcgc catcgatacc ttatgggaca gcttcggcat cgactaccaa gcaacaccgg
     1741 ccgatctcga tgcccgtttc ggaagcagcc gccccaaaat tctcgaaata ggcttcggca
     1801 tggggacggc aaccgcagaa atcgcccgca ggctgcccga aactgacttt ctcgccatcg
     1861 acgtacacgg tcccggcgta ggcaacctgc tcaaactcat agacgaaaac catttagaaa
     1921 acatccgcgt gatgcggcac gatgccgtag aagttgtcga aaatatgctg caagacggct
     1981 cgctcgacgg catccacata ttcttccccg acccgtggca caaaaaacgc caccacaaac
     2041 gccgtctgat acaagccccc ttcatcgcca aactactgcc caaactcaaa accggcggct
     2101 atatccacct ggcgacagac tgggaagaat atgcacagca gatgcttgaa gtcctcagta
     2161 gcttcgacag cctgcaaaat acggcgacag actacgcccc taccccggac taccgccccg
     2221 aaaccaaatt cgaagcgcgc ggcaaacgcc tcggacacgg cgtttgggac ttggtattca
     2281 aacggatcgg ataacagacc gccggataaa aaaatgccgt ctgaagcacg tttgcttaca
     2341 gacggcattt tttcaagata aagcagcgaa cgatgtttcg atacaaagtt caaagcaatg
     2401 gtttgaacgg caaaaacgcg tgtataccgc acgcatcctt gtaggtttta acctgcacat
     2461 cggttttaaa gttggggctg tactagatta gcagatatgt taccctcgaa atatgaagat
     2521 aacgcactgc aaattaaaga aaaaagtaca gaaagaactg ctccgttttt tgtactggaa
     2581 gttaccgccc gttctgccgc cgatattttg ggtatccatc ccaattcggc agcactgttc
     2641 taccgtaaaa tccgcacggt tatcaaccat catttggcct tggctgccaa tgaggttttc
     2701 gagggctctg tcgagttgga cgaaagctat ttcggcggac ggcgtaaagg caaacgtggt
     2761 cgcggtgcgg caggaaaagt ggttgtcttc ggcattctga aacg
//
LOCUS       DAXHKX010000214         2799 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXHKX010000214 DAXHKX010000000
VERSION     DAXHKX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.214"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..364
                     /locus_tag="ACFMWZ_002003"
     CDS             <1..364
                     /locus_tag="ACFMWZ_002003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935896.1"
                     /translation="EVFTSPKLANQMLDLLPSEIWSNPKATFLDPVCKSGVFLREIVK
                     RLDEGLTNQIPDKQTRINHILKNQVFGSTATYVGSFDRYLHKNSFAGERNYFANFKIF
                     LAPNSTMTKTIMPMIKSG"
     gene            complement(283..1083)
                     /locus_tag="ACFMWZ_002004"
     CDS             complement(283..1083)
                     /locus_tag="ACFMWZ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC5935897.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTMAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(1219..1722)
                     /locus_tag="ACFMWZ_002005"
     CDS             complement(1219..1722)
                     /locus_tag="ACFMWZ_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935898.1"
                     /translation="MDFFDFFAQGKYKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGGMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(2059..2700)
                     /locus_tag="ACFMWZ_002006"
     CDS             complement(2059..2700)
                     /locus_tag="ACFMWZ_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC5935899.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT          896 a          599 c          489 g          815 t
ORIGIN      
        1 tgaggttttc acatcgccaa aacttgccaa tcaaatgctg gatttattgc cgtctgagat
       61 ttggtcaaat ccaaaggcga cattcttaga ccctgtgtgt aaatcagggg tatttttgcg
      121 tgaaatcgtc aaacgcttgg atgaaggctt gaccaatcaa ataccagata aacaaactcg
      181 cattaaccac attttaaaaa atcaagtttt tggaagtact gccacgtatg taggtagctt
      241 tgaccgatat ttgcataaaa actcctttgc tggtgaaagg aattattttg ccaattttaa
      301 aatatttctg gcaccaaata gtacaatgac aaagacaatc atgccaatga ttaaatcagg
      361 atagctagaa tgagtcaata acgtcaatgc tcccgccgct atcacaccga tattgatgat
      421 aatgtcattg gatgtaaaaa tcatgctggc tttgatatgg atttctttat tttgattttt
      481 gctcagtaga tataagcaca gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat
      541 cagttgataa ttgggcagct gctcagcacc gataaaacgc ctaatcactt ctatcacccc
      601 aaataacgcc aatattatct gcgttatccc cgccaaaaat gccacacgtt ttttatacgc
      661 catcgtcata ccaatggctg atagcgccaa tatatagaca aagctgtccg ccagcatatc
      721 tagactatca gcaatcagcc ccatagaatt agcaaaaata ccaaccgaac actctatgat
      781 aaaaaacaca aagttaatca tgagcacttg atataataat cttttttcta agtgctcatc
      841 aggcttgtta aacactatct tatcaacaat cacttcggtg gaaatgatat gactatcaaa
      901 attaagcggt tcaagtactt gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa
      961 gcaccgccca gcaatatcaa actgtaattc ataaatatca gacacatctt ttaaacgcat
     1021 gcgaatgagc tgttcttcgg acgggcagtc cattttggta atgttaaaaa tggtcttttt
     1081 catctattta gttccttgtt ttgatcaggt tggctcaaat aaatctgtgt ttatattgct
     1141 gcttggtaat ttttggatgg tttgagtaaa ttgattaggt taaaatttac ctttggaagt
     1201 accgccacgc ataatagttt agatatgttt ataatctctg gataaaaaaa cgtaataagt
     1261 gcttactgga taacaaagtc caaaccaata gcaggcaaaa taaggcatcc cccccccttc
     1321 ttcattaagg atatatattg agaaacaaat cgcaactaaa cagaaaaaac ttgggagata
     1381 aagccatttc attcccctat tcaagaatct agccaagata ggtattttgt attctacaaa
     1441 aaagaaaggc atttccaagg gaaacatgtc agataaaaac ttttgtttat tttttactat
     1501 agatagaacc ttgcttctca agagaaagcc attaataata ccgatgacag ctattaatat
     1561 atagagaata gtataagtat gaataatctt cattagacaa aaagaagaaa tggcagataa
     1621 attacatacg atatattgga atataaaata tttacggtct aaaccttgtt cagttgcaat
     1681 ttttttatac ttaccttgcg caaaaaaatc aaagaaatcc attaaactat ctttcacatt
     1741 agaaatttaa aagctaaata atacgacaaa caatgtgaag tactattcat ggtttattta
     1801 aaaaataata ctattctgaa cattatttag atacagaaat taacaaatta gaactaaaca
     1861 agcttttaaa tactttaatt ttattggaaa gctataaaag gaactataac tttacacact
     1921 agtcacttct ttttaagagg caaaagggat tgggaaggtc gtcttggaga taagcactgg
     1981 tatttcggcc aatggtaaat agagtttacc tcaaataggg tagaacctcc ttcatctgtc
     2041 agttaataac agccactttt acaatgccct gtcgaaataa agtggcacgc ccgatttttc
     2101 actcataccg attaaataat ccatcgtctt ccgagggaac gcgatgccgc gcaccacggt
     2161 cagattcctc aaaacgggga aaaccaaaat atcctccata ccgattccgc cgttgatgcc
     2221 gtctgaagca ccgtccatca aattttccaa ctcttgcaaa tctgcgttta tccgttcgag
     2281 gtattgggcg gttttattca aattggcgga aaagctgccg atgcttttct cttttttgtc
     2341 tgtaaaatat tttaccgctt ccggcgttgc aaattcgggc agcccgattt tgatcacgcg
     2401 cggctgcacc agtttgtcgt tgtatccgcc caccttgtcc agccacgccc gtatctcggg
     2461 gcggacttcg tctttcagac ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc
     2521 gcccataaac gaaccgtctt ctttttgcag gacgggcact tgtttcgcac cgatcatacc
     2581 gatcggcgtt gcctcgtcgt cgtttgccag cacggcttct tcaacgtccg cgccaaacag
     2641 cccggcagcc atccgcgcac gcacacaaaa cgggcaatgg tcgtaaatat acagtttcat
     2701 caaaatattc ctcgtcaacc tgtcggtacc ggctacctta acaccccgcg ccacccgaaa
     2761 caagtttatc ttcccgccta tgcaccgtaa ataaataag
//
LOCUS       DAXHKX010000215         2786 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXHKX010000215 DAXHKX010000000
VERSION     DAXHKX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.215"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            114..437
                     /locus_tag="ACFMWZ_002007"
     CDS             114..437
                     /locus_tag="ACFMWZ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC5935900.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            551..2728
                     /gene="tdfF"
                     /locus_tag="ACFMWZ_002008"
     CDS             551..2728
                     /gene="tdfF"
                     /locus_tag="ACFMWZ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC5935901.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLAGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          678 a          991 c          698 g          419 t
ORIGIN      
        1 gtaagccgca ggcttacatc aaagccgata acgcttccgt tacaactccg cccactgaac
       61 gaagccaaaa cgacaaaccg catcgtaaac cacccaaccc ataggagaac cccatgcaaa
      121 acgaaaccat caacctgaaa cagcaccttg ccgccatcaa agaatactgg cagcccgaaa
      181 tcatcaaccg ccacgggttc caattccgtt tggtcaaact tttgggcgat tacggctggc
      241 atacgcacgg atacagcgac aaagtgctgt ttgccgtgga gggcgacatg gcggtggact
      301 tcgccgacgg cggcagcatg acgatacgcg agggcgagat ggcggtcgtg ccgaagtcgg
      361 tgtcgcaccg cccgcgttcg gaaaacggct gctcgttggt gctgattgag ttgtccgacc
      421 cgtccgaggc cgtctgaaaa cgaagtttcc gaaggaagct gagtttctgc gaagctaaaa
      481 gcagcctgca ccttcaatca atatgccgaa aatacaaccc caccgcacac caacacacaa
      541 aggaaatccc atgacacgct tcaaatattc cctgctgttt gccgccctgt tgcccgtgta
      601 cgcgcaggcc gatgtttctg tttcagacga ccccaaaccg caggaaagca ctgaattgcc
      661 gaccatcacc gttaccgccg accgcaccgc gagttccaac gacggctaca ctgtttccgg
      721 cacgcacacc ccgctcgggc tgcccatgac cctgcgcgaa atcccgcaga gcgtcagcgt
      781 catcacatcg caacaaatgc gcgaccaaaa catcaaaacg ctcgaccgcg ccctgttgca
      841 ggcgaccggc accagccgcc agatttacgg ctccgaccgc gcgggctaca actacctgtt
      901 cgcgcgcggc agccgcatcg ccaactacca aatcaacggc atccccgttg ccgacgcgct
      961 ggccgatacg ggcaatgcca acaccgccgc ctatgagcgc gtagaagtcg tgcgcggcgt
     1021 ggcggggctg ctggacggca cgggcgagcc ttccgccacc gtcaatctgg tgcgcaaacg
     1081 cccgacccgc aagccattgt ttgaagtccg cgccgaagcg ggcaaccgca aacatttcgg
     1141 gctggacgcg gacgtatcgg gcagcctgaa caccgaaggc acgctgcgcg gccgcctggt
     1201 ttccaccttc ggacgcggcg actcgtggcg gcagcgcgaa cgcagccgcg atgccgaact
     1261 ctacggcatt ttggaatacg acatcgcacc gcaaacccgc gtccacgcag gcatggacta
     1321 ccagcaggcg aaagaaaccg ccgacgcgcc gctcagctac gccgtgtacg acagccaagg
     1381 ttatgccacc gccttcggcc cgaaagacaa ccccgccaca aattgggcga acagccgcca
     1441 ccgtgcgctc aacctgtttg ccggcatcga acaccgcttc aaccaagact ggaaactcaa
     1501 agccgaatac gactacaccc gcagccgctt ccgccagccc tacggcgtag caggcgtgct
     1561 ttccatcgac cacaacaccg ccgccaccga cctgattccc ggttattggc acgccgaccc
     1621 gcgcacccac agcgccagcg tgtcattgat cggcaaatac cgcctgttcg gccgcgaaca
     1681 cgatttaatc gcgggtatca acggttacaa atacgccagc aacaaatacg gcgaacgcag
     1741 catcatcccc aacgccattc ccaacgccta cgaattttcc cgcacgggcg cctatccgca
     1801 gcctgcatcg tttgcccaaa ccatcccgca atacggcacc aggcggcaaa tcggcggcta
     1861 tctcgccacc cgtttccgcg ccgccgacaa cctttcgttg atactcggcg gcagatacag
     1921 ccgttaccgc accggcagct acgacagccg cacacaaggc atgacctatg tgtccgccaa
     1981 ccgtttcacc ccctacacag gcatcgtgtt cgacctgacc ggcaacctgt cgctttacgg
     2041 ctcgtacagc agcctgttcg tcccgcaatc gcaaaaagac gaacacggca gctacctgaa
     2101 acccgtaacc ggcaacaatc tggaagccgg catcaaaggc gaatggcttg caggccgtct
     2161 gaacgcatcc gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg cagcaggacg
     2221 cgacccgagc ggcaacacct actaccgcgc cgccaaccaa gccaaaaccc acggctggga
     2281 aatcgaagtc ggcggccgca tcacgcccga atggcagata caggcaggtt acagccaaag
     2341 caaaacccgc gaccaagacg gcagccgcct gaaccccgac agcgtacccg aacgcagctt
     2401 caaactcttc actgcctacc actttgcccc cgaagccccc agcggctgga ccatcggcgc
     2461 aggcgtgcgc tggcagagcg aaacccacac cgaccctgcc acgctccgca tccccaaccc
     2521 cgccgccaaa gcccgcgccg ccgacaacag ccgccaaaaa gcctacgccg tcgccgacat
     2581 catggcgcgt taccgcttca atccgcgcgc cgaactgtcg ctgaacgtgg acaatctgtt
     2641 caacaaacac taccgcaccc agcccgaccg ccacagctac ggcgcactgc ggacagtgaa
     2701 cgcggcgttt acctatcggt ttaaataagg tcgtctgaaa acggagtttc tgcgaagcta
     2761 tagtggatta acaaaaacca gtacgg
//
LOCUS       DAXHKX010000216         2753 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXHKX010000216 DAXHKX010000000
VERSION     DAXHKX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.216"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..286
                     /locus_tag="ACFMWZ_002009"
     CDS             <1..286
                     /locus_tag="ACFMWZ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFC5935902.1"
                     /translation="KKSSVRIGKHAILGTNVTVLPGVTIGDGVAVGAGAIVRKNLEPW
                     TIYVGVDCKPLKRRSQDKILELEKHLVAEFNLDLCPIIPSPNLPKPKETR"
     gene            283..846
                     /gene="gmhB"
                     /locus_tag="ACFMWZ_002010"
     CDS             283..846
                     /gene="gmhB"
                     /locus_tag="ACFMWZ_002010"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC5935903.1"
                     /translation="MKLIILDRDGVINQDRDNFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADGCGCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            875..1618
                     /locus_tag="ACFMWZ_002011"
     CDS             875..1618
                     /locus_tag="ACFMWZ_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC5935904.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            1615..2304
                     /locus_tag="ACFMWZ_002012"
     CDS             1615..2304
                     /locus_tag="ACFMWZ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFC5935905.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPHL
                     SAAALNRWLRDNEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTAHQDMQILLMPS
                     EIRVPEGAPEKQLALLRDFLERQAHSYLIPRLEHHARTTQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQWL
                     KIHGRELFALG"
     gene            2344..2529
                     /locus_tag="ACFMWZ_002013"
     CDS             2344..2529
                     /locus_tag="ACFMWZ_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935906.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT          675 a          874 c          680 g          524 t
ORIGIN      
        1 taaaaaatct agtgttagaa ttggaaaaca tgcaatttta ggaacaaatg taacagtatt
       61 accaggcgtt actattggtg atggggttgc tgttggagca ggggctatag tgagaaaaaa
      121 tcttgagccg tggactattt atgtcggggt agattgtaaa ccactgaagc gcagatcaca
      181 agataaaata ttggagttag agaaacatct tgtagcagaa tttaatctag atttatgtcc
      241 tataatccca tccccgaacc tgcccaaacc aaaggaaacg cgatgaaact catcattctc
      301 gaccgcgacg gcgtcatcaa tcaggaccgc gacaacttcg tcaaatccgt tgacgagtgg
      361 atacctgtcg aaggcagcat ggatgcggtg gcatttctga cgcaggcagg ctgcaccgtc
      421 gccgttgcca ccaaccaatc cggcatcggg cgcaaatatt ttaccgttca aaaccttacc
      481 gaaatgcacg ccaaaatgca ccgcctcgtc cgtcaggcag gcggcgaaat caacggcatc
      541 tggttctgcc cgcacaccga tgccgacggc tgcggctgcc gcaagcccaa accgggtatg
      601 attgaagaca tcatcggacg cttcaacgcc caagcctcgg aaacctggct ggtcggcgac
      661 agcctgcgcg atttgcaggc aatcgatgcc gtcggcggca aacccgcgct ggttctgacc
      721 ggaaaaggca aaaaaacgct ctcccaacac ggacacgaat tgcccgaaca cacacaggtt
      781 ttcgataccc tgctcgattt ctcacaatac atcatgcagg aaaacgccgc accgcaagcc
      841 gactgaacat accgcattcc gacaaggcaa aaccatgctc atcatccgca acctgattta
      901 ctggctgata ctctgttcca ccctgatttt cctctttccc tttatgctgc tcgcctcgcc
      961 tttccgagac ggggcgcaca agatggcgcg ggtctgggtc aaaatcctca acctctcgct
     1021 caaacacatc gtcgggctca aataccgcat catcggcgcg gaaaacatcc ccgaccgccc
     1081 cgccgtcatc tgcgccaaac accaaagcgg ctgggaaacg ctcgcccttc aggacatttt
     1141 tccgccgcag gtttacgttg ccaaacgcga gttgttcaaa atcccctttt tcggctgggg
     1201 cttgaaactg gtcaaaacca taggcataga ccgcaacaac cgccgcgaag ccaacgagca
     1261 gctcataaaa caggggttgg cgcgcaaaaa cgaaggctat tggattacca ttttccccga
     1321 aggcacgcgc cttgcgcccg gaaaacgcgg caaatacaaa ctcggcggcg cgcgcatggc
     1381 gaaaatgttt gagatggaca tcgtccccgt cgccctcaac agcggcgaat tttggccgaa
     1441 aaactccttt ctgaaatatc cgggggaaat caccgtcgtc atctgtccga ccatcccgca
     1501 cgcaagcggc agcgaagccg aattgatgga aaaatgcgaa cacctcatcg aaacgcagca
     1561 gccgcttatt tccggcgcag gcccgtttgc cgccaaaatg ccgtctgaaa ccgcatgacc
     1621 gcctttgtcc acaccctttc agacggcatg gaactgaccg tcgaaatcaa gcgccgtgcc
     1681 aagaaaaacc tgattatccg ccccgccggc acacataccg tccgcatcag cgtcccaccg
     1741 cacctgtccg ccgccgccct caaccgctgg ctgcgcgaca acgaagccgt cctgcggcga
     1801 acactggcaa aaacaccgcc gcacaatacg gcaaaccgac tgcccgaaca tatctggttc
     1861 cacggcaggc agcttgccct caccgcccat caagacatgc aaatcctgct gatgccatct
     1921 gaaatccgcg ttcccgaagg cgcacccgaa aaacagcttg cgctgctgcg ggactttttg
     1981 gaacggcagg cgcacagtta cctgattccc cgcctcgaac accacgcccg caccacacaa
     2041 ctgttccccg cctcctcctc gctgacctct gccaaaacct tctggggcgt gtgccgcaaa
     2101 accacaggca tacgcctcaa ccggcggctg gtcggcgcac cggaatacgt tgccgattat
     2161 gtctgcatac acgaactctg ccacctcgcc cacgccgacc acagccgcgc gttctgggca
     2221 ctgacccgcc gcttcgcacc ctacacgccc gaagcgaaac agtggctcaa aatccacggc
     2281 agggaacttt tcgccttggg ctgacacggt cccgggccgg cccgccgctt tcagacggca
     2341 tccgtgccgg aacaggcacg cgcccgcccg attcaaaccg cgatgacgct ttgccgccgg
     2401 ttcggggcag gatggcggca cacgcgccgt ctgccgcgtt tcatttcaca ccgctcttcc
     2461 gaaacccgaa acccgcccgg tccgacgtgc ggtatgaaac gcttaagctg gcgcgaagtc
     2521 ttttactgat ttgcccgcga aaatgccgtc tgaaaggttt tcggacggca tttttttgcg
     2581 tttcccagga tggcggcgga ttcgtaaaag gcggtcaggg tggattgtag gatgggttga
     2641 gacctgccga atccgccgca tctgccaaat ctaccgccgt cattcctacg aaagtgggaa
     2701 tctagaacgc ggggttaaga aaacctgcat cccgtcattc ccacgaaagt ggg
//
LOCUS       DAXHKX010000217         2707 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXHKX010000217 DAXHKX010000000
VERSION     DAXHKX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.217"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(126..509)
                     /locus_tag="ACFMWZ_002014"
     CDS             complement(126..509)
                     /locus_tag="ACFMWZ_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935907.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(514..1351)
                     /locus_tag="ACFMWZ_002015"
                     /pseudo
     CDS             complement(514..1351)
                     /locus_tag="ACFMWZ_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase domain-containing protein"
     gene            complement(1653..2048)
                     /locus_tag="ACFMWZ_002016"
     CDS             complement(1653..2048)
                     /locus_tag="ACFMWZ_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935908.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(2060..>2707)
                     /locus_tag="ACFMWZ_002017"
     CDS             complement(2060..>2707)
                     /locus_tag="ACFMWZ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC5935909.1"
                     /translation="GAIGAAVGEIVGEALVKNTDFSRMSATEIEKAKAKITAYSKLVA
                     GTASAVVGGDVNTAANAATVAVENNSLFQPQTTLEAGVRNAILRGDIQELRLLLGEAN
                     FSTADAAYAQRILASMEKIGESNSRLLAERYGVDWLNKVHHIFKGHQGSIGNTLIQKS
                     GSMENAVVATQKAVDALKLTKTGNYPVTVTVNGITVIVRVYVNNGVSRIATILKM"
BASE COUNT          805 a          539 c          429 g          934 t
ORIGIN      
        1 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagcttgc tgctgcggta
       61 taaaagtatt tttagtgatt tactgttttt ggtatttttg cccgacgggg ttaaaaatac
      121 agttgctact cttgctgcat gatattggca agatttaatt taaatgtttg ttgaatcttg
      181 tttacatcct tagcaaaaat atgatagtca ctcgaagaat agatagattt tattctatcc
      241 aaattagttt gttgataaca ttttttcatt tccgcgatga aaaaattatc ttttcctctt
      301 tcattgagac agttattttc taacagatag attgcccatg ctgtaaaaat aaatgtacta
      361 tcttccggat attcagataa ataaatttcg aattcaatta tagaatattc agctgcttca
      421 attaatgtta tatctaaatc tctgacaaat ttttccgtta tatttttaaa taatttcttg
      481 aattctatat aattttttat tttttccatg attctatttt cctgtaaaaa tatttaattg
      541 aatatttgga tagcgatttc taaactctaa aataacattg ctgcaagatt gacaggcctt
      601 taattctgta aatagatcaa ttctacctga tacattacga ttatttccga gttgctgtac
      661 tatagtttca agtaatttat attctccatc tatatttctt aatgttcctc taggaggaga
      721 ggcaatatta tgatatttat caacagatat aggtttaaaa atttttgttt caggtaacga
      781 aataaatcca tgttcccctt tttggaaaga actaaatgct tccatccttt gcggtaatcc
      841 tttaacatca atttttgcta tcgcaaaatt ccctatttgt cgatactctt ccggcagttt
      901 catgcggact ctctggcttt cagcagttaa aagcaggagc actgtcattg ctcagataac
      961 gggtaacagg tgtaggcttg gccgatattg cagcctgtgc agatacagat cctgcaacca
     1021 tcaatgtgcc attaacaacc tgaactgtac catgcagata ttctccatta gagattgctt
     1081 tcgctccctg tccaatgtta tatccccctg ctgccacagt agctgctttg acactgaagc
     1141 caagcggtcc gtttaataag taaagtgcag gttgtgcggc aacagctgat ttgtaatcaa
     1201 aagaatcata acgactcaat gtttgatagg ctttcgataa agtgttatta atatctttgg
     1261 aaggaatagt gagattaccc attgactggg gcgttttcag tatcgaagct gcccaagctg
     1321 tgttacctcc agaggcttgg ttaataaaat atgttaccta tgtatattgt ttcttcaaaa
     1381 atcaaccgta ttgtccttgg gtgattttcg taccagcaca atcagtctgc cctagttgac
     1441 agatcgcgcc acactagcca gatacagcgt atcattggta aaactgataa tgcggcaata
     1501 taaagaaaaa cttcaatagt atgttaacga aatttgacgc ataaaaatac gctaaaaaat
     1561 tttcgcttga ctaattaaaa atgggattga gcataaaatt ttaataattt attatattat
     1621 tgaaaatatc tcagattttc agtcagttta ttttataagc cgaaccgtag gctattttcc
     1681 atctttaacc atgaatagac ctcaacttca tcataatttg tgcctgcaat agcaacttcc
     1741 agaaagtcaa taattctacc tgctttattc aaagactctg tagtatatag caggaaagtc
     1801 gctgcatctg acttatccgg tgcagtgatg tctccttcaa aaaaaatagg gatattttcg
     1861 tatttattta gattagttct ttctaaattt acctgaaaat caatgtagca cccacatcct
     1921 gtattatctc tagatactat tattgaattt tctatttgct ctattaaata ttccttccat
     1981 atcatattgt tttcaataat tctacataaa aaagccttct catattcaga taaacaaaac
     2041 tggttcatat attctaccct tacattttca aaatagttgc aatacgagat actccattat
     2101 tgacatatac gcgaacaatt accgttatac cattgaccgt tacagtaact ggataatttc
     2161 ctgtttttgt cagttttaaa gcatccactg ccttttgcgt cgctacaaca gcattttcca
     2221 tggagcctga tttttgaatc aatgtattac cgatgcttcc ttgatgcccc ttaaagatgt
     2281 gatgaacttt atttagccaa tctacaccgt atctttcagc caatagtcga gaattacttt
     2341 cacctatttt ttccatcgaa gctaaaatcc tttgagcata ggcagcatca gctgttgaaa
     2401 aatttgcttc gcccaacaaa agccgtaatt cctgaatatc tcctcgtaaa attgcatttc
     2461 ttactcccgc ttctaatgtg gtttgtggct gaaagaggga attattctct accgccacag
     2521 tagccgcatt cgccgctgta ttcacatccc cgcctacaac ggcagacgca gtgccggcaa
     2581 ccagttttga ataggcagta atcttcgctt tagctttttc gatttcggtc gcactcatac
     2641 gactgaaatc agtattctta accaaagcct caccaacaat ctcaccgact gcagcgccta
     2701 tcgcacc
//
LOCUS       DAXHKX010000218         2692 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXHKX010000218 DAXHKX010000000
VERSION     DAXHKX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.218"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(82..489)
                     /locus_tag="ACFMWZ_002018"
     CDS             complement(82..489)
                     /locus_tag="ACFMWZ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC5935910.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            637..1746
                     /locus_tag="ACFMWZ_002019"
     CDS             637..1746
                     /locus_tag="ACFMWZ_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC5935911.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            1755..2582
                     /gene="fghA"
                     /locus_tag="ACFMWZ_002020"
     CDS             1755..2582
                     /gene="fghA"
                     /locus_tag="ACFMWZ_002020"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC5935912.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSTFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT          746 a          580 c          645 g          721 t
ORIGIN      
        1 ttgcttacaa tccgcttttt aaacaacaaa tttttgattt ctattacgaa caggacaaaa
       61 atcctgctta ttgcactaaa actaagccgt ttcaggaatt tgcggcaaat ttacagcttt
      121 taccgagcct aatgctttcg ctttttggta aaacgccaat ttgtattcaa gcaaatctaa
      181 atagcgtttt aattcggcaa tttgacactt cacattttct atttgatttt caaacaagga
      241 aaggcgttct tcaatggtat cgtcgccaat gacggtacat tccgcaaagc gtttgatgtc
      301 ttttaagctc attcccgtat ttttcaagca ttgcaataag cccaaccatt gcaaatcgtt
      361 atcggtaaaa cagcggttac cgtattcatc acgtccgata ttgggcaaca aaccttcttt
      421 gtcgtaaaaa cgtagggtgt gggcggagat gcctattttt tcggcggctt tggcagtagt
      481 ataagtcatt ttccctcctt ctaaacaaaa acagtaaaaa acacttgctt tagagttaac
      541 tctaaagtgt aaactgttgc tatgttgctc aggcaaggca actttgtcaa tgaattaaga
      601 ggaaagacaa tggaaatgaa acaagccgat tcaaccatca aatctcgtgc ggcggtggca
      661 ttcgccccta accaaccctt acaaattgtg gaaatcgacg tagaaatgcc gcgtaaaggc
      721 gaggtgttaa tccgcaatac ccacactggc gtgtgccata ctgatgcgtt tacgttatca
      781 ggaagcgatc ctgaaggcgt attccctgtg gtgcttggac acgaaggtgc gggtgtggtc
      841 gttgctgtgg gcgaaggtgt gtcaagcgta aaaccgggtg atcacgtgat tccgctttac
      901 accgccgaat gtggcgaatg tgagttttgt tgttcaggta aaaccaactt gtgcgtctca
      961 gtgcgtgata cacaaggtaa aggcttaatg ccggacggca cgacgcgttt ttcttatcaa
     1021 ggtcagccaa tctatcacta tatgggctgt tcgactttca gtgaatactc cgttgttgcc
     1081 gaagtttcac tggcgaaaat caaccctgaa gccaaccatg aacaagtatg tttgctcggc
     1141 tgcggcgtta ccacaggtat tggcgcggtg cataatacgg caaaagtgca agaaggcgac
     1201 tctgttgccg tgtttggctt aggcgcgatt ggtttggctg tggtgcaagg tgcgcgccaa
     1261 gccaaagcag gccgcatcat cgccattgat accaatccat caaaatttga gttggcaaaa
     1321 cagtttggtg caacggattg tttgaacccg aacgattacg ataaaccgat caaagatgtg
     1381 ttgttagaca ttaataaatg gggcattgac catacctttg aatgtatcgg caacgtaaac
     1441 gtaatgcgtc aggcattaga aagtgcacat cgtggttggg gtcaatccat tatcatcggc
     1501 gtagcaggtg caggacaaga aatttcaacg cgtccgttcc agttggtaac aggtcgtgtt
     1561 tggaaaggtt cagcatttgg cggtgtgaaa ggtcgctctg aactgccgaa aatggtggaa
     1621 gattcaatga aaggcgacat tgagttagaa ccgtttgtaa cccacacaat gacactcgat
     1681 caaatcaata aagcctttga cttaatgcac gaaggtaaat cgatccgcgc cgttattcac
     1741 tactaaggta tgcgatgaaa ctgattgaac aacatcaaat ttttggtggt tcgcaacaag
     1801 tttgggcgca tcatgcccaa acgctgcaat gcgaaatgaa atttgccgtc tatttgccaa
     1861 ataatccaga aaatcgaccg cttggtgtga tttattggct ttccggcttg acgtgtacgg
     1921 aacaaaattt cattaccaaa tcaggctttc agcgttatgc ggcagaacat caagtaattg
     1981 tggtggcccc cgataccagc cctcgcggag agcaagtgcc gaacgatgat gcttacgatt
     2041 taggacagag tgcaggcttt tatttgaatg cgaccgaaca gccttgggcg gcgaattatc
     2101 aaatgtatga ttacattttg aacgagctgc cccgtctgat tgagaaacac tttcctacca
     2161 acggcaaacg ttccattatg ggacattcaa tgggcggaca cggcgcattg gtattggcgc
     2221 tgcggaatca ggaacgttat caaagtgttt ctaccttttc gcctatttta tcgccaagcc
     2281 tcgtgccgtg gggagaaaaa gcctttactg cttatttagg gaaagaccgt gaaaaatggc
     2341 agcaatatga tgctaactca ctcattcaac aaggctataa agtgcaaggt atgcgcatcg
     2401 atcaaggctt ggaagatgag tttttgccga cacaattgcg taccgaagat tttatcgaaa
     2461 cctgccgtgc ggcaaaccag ccggtcgatg tgcgtttcca taaaggctac gatcacagct
     2521 attacttcat cgccagtttt attggcgagc atattgctta tcacgccgcg tttttgaagt
     2581 aaaccaaaga gcgttcagtg ttcaaagcag ttttgggata gccggcacga gggcggtaag
     2641 aagtgccggc ataaacgtat gccgtctgag ccgaaaggag ccgactctac gg
//
LOCUS       DAXHKX010000219         2598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXHKX010000219 DAXHKX010000000
VERSION     DAXHKX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.219"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(214..285)
                     /locus_tag="ACFMWZ_002021"
     tRNA            complement(214..285)
                     /locus_tag="ACFMWZ_002021"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:cct)
     gene            complement(305..751)
                     /locus_tag="ACFMWZ_002022"
     CDS             complement(305..751)
                     /locus_tag="ACFMWZ_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC5935913.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(756..1730)
                     /gene="ispB"
                     /locus_tag="ACFMWZ_002023"
     CDS             complement(756..1730)
                     /gene="ispB"
                     /locus_tag="ACFMWZ_002023"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC5935914.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1954..2262
                     /gene="rplU"
                     /locus_tag="ACFMWZ_002024"
     CDS             1954..2262
                     /gene="rplU"
                     /locus_tag="ACFMWZ_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC5935915.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2287..2559
                     /gene="rpmA"
                     /locus_tag="ACFMWZ_002025"
     CDS             2287..2559
                     /gene="rpmA"
                     /locus_tag="ACFMWZ_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC5935916.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          764 a          698 c          575 g          561 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacggagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa
      121 gttttcagat ggcaaaaaag ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc
      181 aacacgggaa acagacaaat gcaaatcgtc aaacctcgcc aacaggaatc gaacctgtat
      241 tttacgctta ggaggcatac gttctatccg ttgaactatg gcgagccgaa atgaaaagga
      301 gattttaacc ctttccgacg acaaaagaca agatgccggc cgcaatcagc ggcccgacag
      361 ggataccgcc cataaatgcc accccgataa ccgtcccgat taacagccct gtaattaaaa
      421 caggctgctg tcccatcaaa ggcacgccgc gtcccgccag ccaagccacg aaaataccga
      481 taaaaacggc ggatatcatt ttaaaattca aaaattcggc aacgggagga acctgcgcct
      541 ttcctgaaac caacggactc aaaaccccta tggtcaaaag aatgataccg agattcaacc
      601 catgcttctc gaccaacggg acaaactgta ccaatgccgt ctgctgcatc agcagcaata
      661 tggttgccga gatggtaatc gaattgttgt tgctgaccac ccccaacaga atcagcgtaa
      721 ccagaaacag gggaacaaaa ctgaaattca tcgcctcaag acaccctgac caaagattcc
      781 ttcgccagct gaatcatggc atccttcact tcgctgtcgg gcagggcatc caaggcggta
      841 acggcacaat cgactgcttt gcgcgcctcg cctatcgaat atgccaaagc atccgaacgg
      901 acgacataat cgtggatttt ctcaaaatag ctgcgatctg cattttccaa agcagtacgc
      961 acatcgttcg caacctgttc ggaaccctga cgcatcagat aaatcaaagg caaagtcggt
     1021 tttccttccg ccaaatcgtc gccgacgttt ttgccggttt cttcggtttc gccagaatag
     1081 tccagcacat cgtcaataat ttggaatgcc gtaccgacat acataccgta gtctttcaac
     1141 gcccgttcgt gttcggggga agccttgccc aaaattgcgc cgacttgagc ggcagcttca
     1201 aacaattttg ccgttttata ttggatgact tggatatatt gttcttcggt aatgtccgta
     1261 ttgccgatgt tcatcagctg catgacttcg ccctcggcaa taatgttggt tgcatccgcc
     1321 atcacttcca aaacgcgcat actgcccgag gcaaccatca gttgaaaggc gcgcgtgtat
     1381 aaaaagtcgc caaccaacac agccgccgca ttgccgaaca gattgtttgc cgttgcccgc
     1441 ccacggcgca aatcgctttc atcgacgaca tcgtcgtgca ggagggtgga agtgtggata
     1501 aactcgacca tcgccgccag cgaatacagt ttctcgtcat cataaccgac cgccttaccc
     1561 gccaaaatcg tcataatcgg acgcaggcgt ttgccgcccg cgctgatgat atatgtaccg
     1621 atttgcgaaa tcagtgcgac atcggattgc accgcacggt tgatgacttc attgactttg
     1681 gcaaggtctt caggcagatg tcgctggaaa tagggcagat tctcgagcat ggcagactct
     1741 caggtttacg tttccgtcaa acggcagata ccgcttgaaa gctgattcaa atagcgggca
     1801 attatatcag caaagccatc agggtacatc ttttcagacg gcatatggtt atccgaaccg
     1861 cgcaaacttt gacaaaacag gcaaataaca atacaatgcc gagttccgca caatggtgtg
     1921 cggatattaa ccataaccct tatggagttg agtatgtacg cggtcgtaaa aaccggcggc
     1981 aaacagtata aagtttccgt cggcgaaaaa ttgaaagtag aacagatacc agccgaactc
     2041 gacagccaaa tcgaactgac cgaagttttg atgattgctg acggcgaatc tgtaaaagtt
     2101 ggcgcaccct ttatcgaagg tgcaaaagta acggctaaag tagtggcaca cggtcgtggc
     2161 gaaaaagtcc gcatcttcaa aatgcgccgc cgcaaacact accaaaaacg ccaaggccac
     2221 cgccaaaatt tcacccaaat cgaaatcgtg gcaatcgcct aaccttaaaa cttaggagca
     2281 ttacaaatgg caagtaaaaa agcaggcggc agcacccgca acggtcgcga ttcagaagcc
     2341 aaacgcttgg gcgttaaagc ctacggcaac gagctgattc ccgcaggttc catcatcgta
     2401 cgccaacgcg gtaccaaatt ccacgcaggc gacaacgtag gtatgggcaa agaccacact
     2461 ttgttcgcca aagttgacgg ttatgtcgaa ttcaaaacca aaggcgcgct gaaccgtaaa
     2521 actgtcagca tccgtcctta caccggttct gaagaataat ccaaccgata cctgaagccg
     2581 catcttttca cgatgcgg
//
LOCUS       DAXHKX010000220         2594 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXHKX010000220 DAXHKX010000000
VERSION     DAXHKX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.220"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            646..2445
                     /locus_tag="ACFMWZ_002026"
     CDS             646..2445
                     /locus_tag="ACFMWZ_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFC5935917.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANTTDEEDEEYLEPVKRTAVVLSFRSDKEGTGEKEVTEDSHWGVYFDEKGVLKA
                     GTITLKAGDNLKIKQNTDENTDENTNASSFTYSLKKDLTDLTSVETEKLSFGANGNKV
                     NITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAA
                     SIKDVLNAGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKR
                     TEVKIGAKTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTT
                     TANGQTGQADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQL
                     QNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSM
                     TPQFSSVSLGAGADAPTLSVDDKGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLK
                     GVAQNLNNRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGY
                     SSISDGGNWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          774 a          636 c          633 g          551 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatccg tttgcgcccg ccaagccgtg ttgcacagat
       61 gcgacacggc ttttttcttt ctgttgtcca aatgtaaatt ttcgtcaaaa aacatcaaaa
      121 atcacccccc cgaatttgcg taaaataaag tgtaatccct ttgatcataa gggaatgttg
      181 acaattagat cgattctcct aggctgactt tcgcaataaa tggggttgtc aataattgtg
      241 tatggtcgtt tgccgtccgg cggccgtcat tcccgcgaac gcaggaatcc agcaacacaa
      301 tgcagcagga ctttatcgga aaaaacagaa accccaccgc cgtcattccc gcgcaggcgg
      361 gaatccagca acacaacgca gcaggacttt atcggaaaaa accgaaaccc caccgaccgt
      421 cattcccgca aaagcgggaa tccagacccg tcggcacgga atcttaccgg ataaaacagt
      481 ttccttagat tccacgtccc agattcccgc cttcgcggga atgacgagat tttaagttgg
      541 gggaatttat cagaaaaccc ccaaccccca aaaccgggcg gatgccgcac catccgcccc
      601 caaaccccga tttaaccatt caaacaaacc aaaagaaaaa acaaaatgaa caaaatatac
      661 cgcatcattt ggaatagtgc cctcaatgcc tgggtagtcg tatccgagct cacacgcaac
      721 cacaccaaac gcgcctccgc aaccgtggcg accgccgtat tggcgacgct gttgtccgca
      781 acggttcagg cgaatactac cgatgaagaa gacgaagaat atttagaacc cgtaaaacgc
      841 actgctgtcg tgttgagctt ccgttccgat aaagaaggca cgggagaaaa agaagttaca
      901 gaagattcac attggggagt atatttcgac gagaaaggag tactaaaagc cggaacaatc
      961 accctcaaag ccggcgacaa cctgaaaatc aaacaaaaca ccgatgaaaa caccgatgaa
     1021 aacaccaatg ccagtagctt cacctactcg ctgaaaaaag acctcacaga tctgaccagt
     1081 gttgaaactg aaaaattatc gtttggcgca aacggtaata aagtcaacat cacaagcgac
     1141 accaaaggct tgaatttcgc gaaagaaacg gctgggacga acggcgacac cacggttcat
     1201 ctgaacggta tcggttcgac tttgaccgat acgcttgcgg gttcttctgc ttctcacgtt
     1261 gatgcgggta accaaagtac acattacact cgtgcagcaa gtattaagga tgtgttgaat
     1321 gcgggttgga atattaaggg tgttaaaact ggctcaacaa ctggtcaatc agaaaatgtc
     1381 gatttcgtcc gcacttacga cacagtcgag ttcttgagcg cagatacgaa aacaacgact
     1441 gttaatgtgg aaagcaaaga caacggcaag agaaccgaag ttaaaatcgg tgcgaagact
     1501 tctgttatta aagaaaaaga cggtaagttg gttactggta aaggcaaagg cgagaatggt
     1561 tcttctacag acgaaggcga aggcttagtg actgcaaaag aagtgattga tgcagtaaac
     1621 aaggctggtt ggagaatgaa aacaacaacc gctaatggtc aaacaggtca agctgacaag
     1681 tttgaaaccg ttacatcagg cacaaatgta acctttgcta gtggtaaagg tacaactgcg
     1741 actgtaagta aagatgatca aggcaacatc actgttatgt atgatgtaaa tgtcggcgat
     1801 gccctaaacg tcaatcagct gcaaaacagc ggttggaatt tggattccaa agcggttgca
     1861 ggttcttcgg gcaaagtcat cagcggcaat gtttcgccga gcaagggaaa gatggatgaa
     1921 accgtcaaca ttaatgccgg caacaacatc gagattaccc gcaacggtaa aaatatcgac
     1981 atcgccactt cgatgacccc gcagttttcc agcgtttcgc tcggcgcggg ggcggatgcg
     2041 cccactttga gcgtggatga caagggcgcg ttgaatgtcg gcagcaagga tgccaacaaa
     2101 cccgtccgca ttaccaatgt cgccccgggc gttaaagagg gggatgttac aaacgtcgca
     2161 caacttaaag gcgtggcgca aaacttgaac aaccgcatcg acaatgtgga cggcaacgcg
     2221 cgtgcgggca tcgcccaagc gattgcaacc gcaggtctgg ttcaggcgta tctgcccggc
     2281 aagagtatga tggcgatcgg cggcggcact tatcgcggcg aagccggtta cgccatcggc
     2341 tactccagta tttccgacgg cggaaattgg attatcaaag gcacggcttc cggcaattcg
     2401 cgcggtcatt tcggtgcttc cgcatctgtc ggttatcagt ggtaagggct ttttcgcctg
     2461 tctgctgttg gggcaggcgg aaggtttgaa gggaaaggtg gcgatttgcc gcctgagacc
     2521 tttgcaaaat tccccaaaat cccctaaatt cccaccaaga catttagggg atgttccatg
     2581 agcaccttct ttca
//
LOCUS       DAXHKX010000221         2567 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXHKX010000221 DAXHKX010000000
VERSION     DAXHKX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.221"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            244..2469
                     /locus_tag="ACFMWZ_002027"
     CDS             244..2469
                     /locus_tag="ACFMWZ_002027"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC5935918.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFTRHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKHP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNGKSPKRKAGELDNRGSHFYLTLYWAQELAEQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAVDIGGYYAANPEKAAQAMRPSVTFNQALNA
                     L"
BASE COUNT          710 a          783 c          593 g          481 t
ORIGIN      
        1 aatttaaacc actataaaat caatttcacc gaaaatatgc cgccgcacat caggcacagc
       61 acctccgaca ccgccgccaa aagcgggaaa accatttccc ccttcctttc acgccgccgc
      121 gttcaaacac gccaagatcg aacaaagcgc gaacaaagcc gccaaaatat gccataatag
      181 ccacgcacat tccccgcgca atgtgttcca aaagaaaaaa ccaacctaca agagaataat
      241 cctatgactc aaaaatccac cattgtttat acccataccg acgaagcccc cgcgctggcg
      301 acccaatcgc tgctgccgat tgtgcaggct tttacccgcc acgccgatat tgatgtcaaa
      361 actgccgaca tttccctgtc cggccgtatt ttggcggcat tccccgaata tctgaccgaa
      421 gcgcaacgcg tacccgacgc gcttgccgaa ttgggcgaac tggtaaaaca acccgatgca
      481 aacgtaatca aactgccgaa catcagcgca tccgtaccgc aactgaccgc cgcgattaaa
      541 gaattgcagt ctaaaggctt tgccgttccc gattatcctg ccgacccgca aaccgatgaa
      601 gaaaaagccg tgcgcgaacg ctacgaccgc atcaaaggca gcgcggtaaa ccctgtcctg
      661 cgtgaaggca actccgaccg tcgcgcgcct aaagcagtga aaaactttgc gaaaaaacat
      721 ccgcacagca tgggcgcatg gaccaaagac tccaaaaccc acgtcgccac catgcaaagc
      781 ggcgattttt tccataacga acaatccgtt accgtacctg aagcgacttc cgtatccatc
      841 gtattcaccg acaaacaagg caataaaaaa gagctgcgcg cgcctgttgc cctgaaagcc
      901 ggcgaaatca tcgacgcaac cgtgatgagc aaaaaagccc tgctcgcctt ccttgccgaa
      961 caagtcaaag acgcgaaagc aaaaggtgta ttgttctcgc tgcacatgaa agccaccatg
     1021 atgaaagtgt ccgacccgat tatcttcgga catgccgtca aagtattctt cgcgcctgta
     1081 tttgaaaaat tcggcgacaa actggctgct gccggcgtca acgttaacaa cggcttcggc
     1141 aacctgcttg ccaatctgga caaactggat gcggacaccc gcaccgccgt cgaagccgaa
     1201 atcgccgccg tttacgccgc caaccccgat ttggcgatgg ttgattccga caaaggcatc
     1261 accaacctgc acgttcccag cgatgtcatc gtcgatgcct ctatgcctgc gatgattcgc
     1321 aattccggcc gtatgtggga caaaaacggc aaagcgcaag acaccaaagc cgtgattccc
     1381 gacagcagct atgccggcgt ttaccaagca accatcgact tctgccgcga acacggcgcg
     1441 ttcgatccga caaccatggg tactgtgccc aacgtcggac tgatggcgca agcggcggaa
     1501 gaatacggct cgcacaacaa aacctttgaa atcgaagccg acggtcaggt tcaagtcatt
     1561 gatgcggaag gaaaagtcct gatgcagcac gacgttgaag caggcgacat ctggcgtatg
     1621 tgccaaacca aagacgctcc ggtcaaagac tgggtgcaac tcgccgtcaa ccgcgcccgc
     1681 ctgagcaaca cgcctgccgt gttctggctc gatgaaaacc gtccgcacga caagagcctg
     1741 ctcgccaagg ttaaagccta ccttgccgaa ctggatacca atggcctcga catccgcgtc
     1801 ctcgctcctg aagaagccgc caagttcagc ttgggtcgtc tgaaaaacgg cgaagacacc
     1861 atctccgtaa ccggtaatgt cttgcgcgac tacctgaccg acttgttccc aattttggaa
     1921 ctcggcacaa gcgcgaaaat gctgtctatc gttccattaa tgaacggcgg cggtatgttt
     1981 gaaaccggcg cgggcggttc tgcaccgaaa cacgttcaac agttcctcga agaaaaccac
     2041 ctgcgttggg actctttggg cgaattcctc gcactcgccg tatcgtttga acatttggcg
     2101 caaaaaaccg gcaatgccaa agcccaagtc ctcgctgaca ctttggatgc cgccaccgaa
     2161 aaactgctgt tgaacggcaa atcgcctaaa cgtaaagccg gcgaactcga caaccggggc
     2221 agccatttct accttaccct ctactgggcg caagaattgg cggagcaaga caaagatgcc
     2281 gaactgaaag ccgcatttac gccattggca gccgctttga ccgccgacga agcgaaaatc
     2341 gttgccgagc tttccgccgt acaaggcaaa gcggtcgaca tcggcggcta ctacgcagcc
     2401 aatcctgaaa aagcggcaca agcgatgcgc ccgagcgtaa cctttaatca ggcactaaac
     2461 gccttataag cacaaaggta aaaatgccgt ctgaacattc gtcgttcaga cggcattagt
     2521 tatccctatc tgcctgatta tgattaaacg ccgcacccat tggatat
//
LOCUS       DAXHKX010000222         2550 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXHKX010000222 DAXHKX010000000
VERSION     DAXHKX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.222"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..279)
                     /locus_tag="ACFMWZ_002028"
     CDS             complement(<1..279)
                     /locus_tag="ACFMWZ_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935919.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALIN"
     gene            complement(284..418)
                     /locus_tag="ACFMWZ_002029"
     CDS             complement(284..418)
                     /locus_tag="ACFMWZ_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935920.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGFALKILMGKKLMHDITRQHKRK
                     "
     gene            complement(433..1488)
                     /gene="tal"
                     /locus_tag="ACFMWZ_002030"
     CDS             complement(433..1488)
                     /gene="tal"
                     /locus_tag="ACFMWZ_002030"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC5935921.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            1585..>2550
                     /locus_tag="ACFMWZ_002031"
     CDS             1585..>2550
                     /locus_tag="ACFMWZ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC5935922.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLST
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
BASE COUNT          527 a          657 c          741 g          625 t
ORIGIN      
        1 attaatcaaa gccattatct catctttatt cagttccatc ccgggttctt caagtaaggt
       61 taaatcatat agggcattat attgctcttc ggtagctgaa ccatccataa ggccggacga
      121 gaaaaaatcg aaggctctat ctgcgattct ctcattactt gcatttctac taaccagttt
      181 catcaattct gtatatattg tatggggagg taccggatcc ttaaaaatca gatctgaata
      241 aatttcattg ggtgaaatga tttcgagtgc ttttgctatg gttctatttc cttttgtgtt
      301 ggcgggtaat gtcgtgcatt aacttcttgc ccattaatat ttttagggcg aatccttgat
      361 atgccgcact gtgtccggtc aaaagggcga tgccgtctga aagcctttca gacggcatcg
      421 gggatgaaaa gtttaaacca acggtgcgag cagtttttca aacgcttctt caaactgttt
      481 caaaccgtct tcctgcaaac gcgttgccaa ggtttcgaca tcgatgccga gcgcggcggt
      541 ttcggcgagc tgcgcttgtg cttcttctac gccttcggtc agcgtggctt tggctgtgcc
      601 gtggtcgata aaggctttga gcgtggcatc gggaacggtg ttgacggtgt gcgcgccgat
      661 caggctgtca acgtagagcg tgtcgggata ggctgggttt ttcacgccgg tagatgccca
      721 taaaagctgc acgcggtttg cgccttgggc ttccagtgcg gcaaattcgg gggcggtgaa
      781 atattgttcc caatcttgat aggcggcttt ggcaagggcg atggcgattt tgcctttgag
      841 ccggtcgggc agtgttgtgt ccagcgcact gtccacacgc gagatgaaga agctggcgac
      901 gacgtggata tgggcaacgc tttgtccggc tgccaagcgt ttggcaatgc cgcgcgcgta
      961 ggcggcgtag gctttgaggg tttgggcgcg tgagaacagc agcgtgaggt ttacgctgat
     1021 gccgtctgaa acgagggttt cgagcgcatc gatgcctgcg tcggtggcag gcactttaat
     1081 catcgcgttt ttgcacccga tggcggcgta gaggcggcgc gcttcttcta ccgtgccttg
     1141 cgcgtctttg gacaattcgg gcgaaacttc gaggctgacg aaaccggttt tgccgccggt
     1201 ggattcgtgt tcggcaaggc agacgccgca ggcggcacgc acatcggcaa ccgccatcgt
     1261 ttcgtagcgt tgtttggggg tcaggtcttg ctgcttgagg gcggcaacct cgtcggcgta
     1321 aagcgcgtcg cctgcgaagg ctttttggaa aatggcggga ttggaagtta cgccgcacac
     1381 gccttgtttc aacatttgcg ccaattcgcc gctttgcacg agcgagcggg aaaggttgtc
     1441 cagccagatt tgttgtccta atgctttaac gtccgataaa atagtcattt ctgattcctt
     1501 tggatggatg tgtatgtttc aaaggcttat gctaccccga ttcggaaatt ttgggtagtt
     1561 ttattacagc aaaggcggat ggcaatggca gaaaacggaa aatatctcga ttgggcgcgc
     1621 gaagtgttgc acaccgaagc ggaaggcttg cgcgaaattg cggcggattt ggacgaaaac
     1681 ttcgcccttg cggcggacgc gttgttgcac tgcaaaggca gggtcgttat cacgggcatg
     1741 ggcaagtcgg gacatatcgg gcgcaaaatg gcggcaacca tggcctcgac cggcacgcct
     1801 gcgtttttcg tccaccctgc ggaagcggca cacggcgatt tgggtatgat tgtggacaac
     1861 gacgtggtcg tcgcgatttc caattccggt gaaagcgatg aaatcgccgc catcattccc
     1921 gcactcaaac gcaaagacat taccctcgtc tgcatcaccg cccgccccga ttcaaccatg
     1981 gcgcgccatg ccgacatcca catcacggcg tcggtttcca aagaagcctg cccgctgggg
     2041 cttgccccga ccaccagcac caccgccgtc atggctttgg gcgatgcgtt ggcggtcgtt
     2101 ctgttgcgcg cgcgcgcgtt cacgcccgac gacttcgcct tgagccaccc tgccggcagc
     2161 ctcggcaaac gcctactttt gcgcgttgcc gacattatgc acaaaggcgg cggcctgcct
     2221 gccgtccgac tcagcacgcc cttgaaagaa gccatcgtca gcatgagtga aaaagggctg
     2281 ggcatgttgg cggtaacgga cgggcaaggc cgtctgaaag gcgtattcac cgacggcgat
     2341 ttgcgccgcc tgtttcaaga atgcgacaat tttaccggtc tttcgataga cgaagtcatg
     2401 catacgcatc ctaaaaccat ctccgccgaa cgtctcgcca ccgaagccct gaaagtcatg
     2461 caggcaaacc atgtgaacgg gcttctggtt accgatgcag atggcgtgct gatcggcgcg
     2521 ctgaatatgc acgacctttt ggcggcgcgg
//
LOCUS       DAXHKX010000223         2521 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXHKX010000223 DAXHKX010000000
VERSION     DAXHKX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.223"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            116..673
                     /locus_tag="ACFMWZ_002032"
     CDS             116..673
                     /locus_tag="ACFMWZ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC5935923.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAVADIARTYSAHY
                     LNPNNRNMTLFPDALPCLDTLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGICGELGFDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LLSAPHLAVLDGLSELPGFLAQHYA"
     gene            complement(760..1728)
                     /gene="ispH"
                     /locus_tag="ACFMWZ_002033"
                     /gene_synonym="lytB"
     CDS             complement(760..1728)
                     /gene="ispH"
                     /locus_tag="ACFMWZ_002033"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC5935924.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGEEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1757..2284)
                     /gene="lspA"
                     /locus_tag="ACFMWZ_002034"
     CDS             complement(1757..2284)
                     /gene="lspA"
                     /locus_tag="ACFMWZ_002034"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC5935925.1"
                     /translation="MVLFYRLLKNMSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQ
                     YRERVNVIPSFFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEF
                     ATLGKTGAAMIIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLA
                     VLDNIVHRKTQEEKY"
BASE COUNT          571 a          759 c          630 g          561 t
ORIGIN      
        1 gctgccgcct aaaaggcggc tcggatgcct gattatcggg tatccgggga ggattcaggg
       61 ggtatttggg tagaattagg aggtatttgg ggcgaaaaca gccgaaaacc tatgtttggg
      121 tttcggctgt cgggagggaa aggaattttg caaaggtctc agcctgcccg aaatcatccg
      181 cgacctgctc gaaatgccgt ctgaaaccgc cgttgccgac atcgcacgca cctattccgc
      241 gcattacctc aatcccaaca accgcaatat gacgctcttt cccgatgccc tgccctgtct
      301 ggacacgctc aaagcacaag gatactggct tgccgtcgcc acgggcaaag ggcgggcggg
      361 cttggacaac gccatcagcc aaaccgccac cggcggctat tggctcgcca ccgcctgcgc
      421 gggggaatat ccctccaaac cctcgcccga aatggtattc ggaatctgcg gcgaactggg
      481 gttcgacccg aaagaggcat tggtcgtcgg cgatacggcg catgacctgc atatggcggc
      541 aaacgcaggc gcggcggcag tcggcgttgc caccggcgca cattcgcgcg aacagctcct
      601 tagcgcaccg catctcgccg tattggacgg tttgtccgaa ctgcccggtt ttcttgcaca
      661 gcattacgcc tgattggttt ccgcatccgg cactcgaaac aaatgccgtc tgaagcctgt
      721 tcagacggca tttgttttgc ccaaacattc aacgcttgct caacgtttgc acaaatcggg
      781 tttggtttcg ccctcgcggc gcagctcttt gggcaggacg aacacaatgc tttcttcctc
      841 accctcgcct tcgcgtacgg tttcgtgccc ccatccgcgt atggttgcca gtacttcccg
      901 caccaacact tcgggcgcgg acgcgcctgc cgttacgccg actttgtttt tgccctcaaa
      961 ccatgcgcgt tgcaggtagc ctgcattatc caccatatac gcatcgattc cgcgcgatgc
     1021 cgccacttcg cgcaagcggt tgctgttgga cgaattgggc gaaccgacca caatcacgat
     1081 gtcgcactgt tctgccaact ctttgacggc ggtttgccgg ttggtcgtcg catagcagat
     1141 atcttccttg tgcggattgc ggatattggg gaaacgcgcg ttcagcgcgg cgatgatgtc
     1201 tttggtttca tcgaccgaga gcgtggtttg gctgacatag gcgagtttgt cggggtttct
     1261 gacttcgagt tttgccacat ctccgaccgt ttcgaccaaa agcattttgc ccggcgcaag
     1321 ctgccccatc gttccttcaa cctcgacgtg ccccttatgc ccgatcatga tgatttcaca
     1381 gtcttgggca tccagtcggg cgacttcctt atgcactttc gtcaccagcg ggcaagtcgc
     1441 atcaaacacg cggaaaccgc gctccgccgc ttcttgccgc accgccttcg atacgccgtg
     1501 tgccgaataa accagtgtcg cgcccggcgg cacttccgcc aagtcttcaa taaacaccgc
     1561 acctttttca cgcaggttgt ccacgacgaa tttgttgtga acgacttcgt ggcgcacata
     1621 aatcggcgcg ccgaactctt ccaaagcacg ttcgacaata ctgattgccc gatccacacc
     1681 agcgcagaag ccgcgcggat tggcaaggat gatggttttc tcgttcataa gcccggtatt
     1741 tcgttttcag acggcatcaa tatttttctt cttgggtttt acggtggacg atgttgtcca
     1801 acaccgccaa caccgcaccg acgcagataa agctgtcggc aatattaaag gcgggataaa
     1861 accaattttg ccaataaaac aataagaaat cgacgacatg accgtgtatc aggcggtcga
     1921 tgacattgcc taacgcaccg ccgataatca ttgccgcacc cgttttgccg agggttgcaa
     1981 actcatcgcg caagatggcg cgtaccaaat acgcgctcac cgccaccgcc agcaccaaaa
     2041 aaaagtattt ttgccagccg ccctgatcgg caaggaagct gaacgccgca cccgggttgt
     2101 acaccagcgt cagatcgaaa aaggaaggaa tgacattgac gcgttcccga tactgaaacg
     2161 acgacagcac cgcccacttc gaccactggt ccagcacgat ggcggcaagt gccaataccc
     2221 aatagcgcgt tttacttgaa acagatgaag acatattttt caacagccgg taaaagagta
     2281 ccattttacc cgaaaacccc ctttcctgta cccgaaacgg caaatgccgt aatcttaaaa
     2341 cccgtcattc ccgacaacac cgtaatctcg aaacccgtca ttcccgcgta ggcgggaatc
     2401 cagacctgtc cgcacagaaa cttatcggat aaaaacagtt gctcaaaccc ctcgttctag
     2461 attcccactt tcgtgggaat gacggttcag ttgcgttcca acaacaccgc aatctcgaaa
     2521 t
//
LOCUS       DAXHKX010000224         2507 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXHKX010000224 DAXHKX010000000
VERSION     DAXHKX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.224"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            35..1390
                     /locus_tag="ACFMWZ_002035"
     CDS             35..1390
                     /locus_tag="ACFMWZ_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC5935926.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLFYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLIITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMMGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2422
                     /locus_tag="ACFMWZ_002036"
     CDS             1394..2422
                     /locus_tag="ACFMWZ_002036"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC5935927.1"
                     /translation="MSDTPATPATPATRDFGLIDGCAVTGYVLSNRRGTRVCVLDLGG
                     IVQEFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRV
                     EANEGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDE
                     DNRLTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVS
                     DDLEVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIY
                     SDRNGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATI
                     AYGIESLS"
BASE COUNT          473 a          680 c          742 g          612 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt taggcggccg
      301 ccgtctgccg tatctgtttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcgaa tatggcgatg cagccgttta agatgatggt
      481 cggcgatatg gtcaacgagg agcagaaaag ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg ttcgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccaca aaccgtggtc gtagcattct atgtgggcgc
      661 ggcattactg attattacca gtgcgttcac aatcttcaaa gtcaaagaat acgacccgga
      721 aacctatgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactttta aaaacggcac ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccact gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgccgcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tcccgatgat gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccccgct acccccgcta cccgcgattt
     1441 cggcctgatc gacgggtgtg ccgtaaccgg ctatgtgctg tccaatcggc gtggtacgcg
     1501 tgtctgcgtg ctggacttgg gcgggattgt gcaggaattt tccgttttgg cagacggcgt
     1561 gcgcgaaaac ctcgtggtgt cgttcgacga tgcggcttcc tatgcggaca atccgtttca
     1621 gattaacaag cagatagggc gcgtggccgg acgcatccgc ggtgcggcgt tcgacatcaa
     1681 cggcaggact taccgcgtgg aggccaacga aggcaggaac gcgctgcacg gcggttcgca
     1741 cgggctggcc gttacccgtt tcaacgcggt ggcggcagac ggccgttcgg tggtgctgcg
     1801 cagccgcctg caacagtcgg ccgacggtta tcccaacgat ttggatttgg atatttccta
     1861 ccgcttggac gaggacaacc ggcttaccgt tagctatcgc gccaccgcgc tcggcgacac
     1921 ggtgttcgac ccgacgctgc acatttactg gcggctggac gcgggcctgc acgatgcggt
     1981 tctgcatatt ccgcagggcg gacatattcc ggccgatgcc gaaaaactgc ccgtctcaac
     2041 ggtttcagac gacctcgaag tatttgattt cagccggccc aagccgctgg atgccgccgt
     2101 tgccgccctg cgccgcgaaa cgggtcgggc cggttttgac gacgcttacc gcgtgccgtc
     2161 cgatataggc cgtcccgccg ctgtgttgca agccggacgc cgccgtcgta tcagcatata
     2221 cagcgaccgc aacggcttgg tcatctttac cgccgccccg caggatttcg cgcggcacga
     2281 tgcgggcgtt tacgacgcgc tggcgaccga ggcgcagacg ctgcccgaca gcctgaattg
     2341 gcccgagttc ggcaatattc gtctgaacaa gggtgatacc agggaggcga cgattgctta
     2401 cggcatcgaa tccctttctt aggagcttcc taacaccggt tgcagacggc ctttttatag
     2461 tggattaaca aaaaccggta cggcgttgcc tcggcttagc tcaaaga
//
LOCUS       DAXHKX010000225         2468 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXHKX010000225 DAXHKX010000000
VERSION     DAXHKX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.225"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(13..819)
                     /locus_tag="ACFMWZ_002037"
     CDS             complement(13..819)
                     /locus_tag="ACFMWZ_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC5935928.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="ACFMWZ_002038"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="ACFMWZ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC5935929.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          675 a          625 c          648 g          520 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcgaaga atgtgccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gtgggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgag ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggcaggtt tgcagggcgc gggcaagacg
     1381 acgaccgccg gcaaactcgc ccgcctgctg aaaaacgatc agaagaaaaa ggttttggtc
     1441 gtatctgccg acgtttaccg ccctgccgcg attgaacaac tgcgtctgtt ggccgaacag
     1501 gtcggcgtgg actttttccc atccgatacc aaccaaaaac cggttgagat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgtattga tggtcgatac cgccggccgt
     1621 ttggcaatcg atgaggagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccggt gtcgtattga ccaagatgga cggcgattcg
     1801 cgcggcggcg cagctttgtc cgtgcgccac gtaaccggca agccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgtcc aaaaaggtat tgatgaagaa
     1981 gccgccgcca aaatggcgaa aaagctacaa aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat atcgaaacaa atccccgaag gaacggctga aaaagcgatg
     2161 ggcaaagtgg aagccatcat caactcgatg actccaaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacgaccgt acaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaagg gaatgaaagg aatgttcccg
     2401 ggtttgtaag ccgatttgac cgaaaacgcc gtctaaaatt tcagacggcg tttttgtttt
     2461 atattctg
//
LOCUS       DAXHKX010000226         2338 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXHKX010000226 DAXHKX010000000
VERSION     DAXHKX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.226"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            43..2256
                     /locus_tag="ACFMWZ_002039"
     CDS             43..2256
                     /locus_tag="ACFMWZ_002039"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC5935930.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          599 a          755 c          565 g          419 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac gaaagaccca tcatgaccgc catcagcccg
       61 attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg aatggttcga cagctactgc
      121 gccgcgctgc cggacaacga taaaaaactt gtcttagccg cccgttcgct ggcggaagca
      181 cattaccccg ccgatgccgt cacgccgtat ggcgagccgt tgtccgacca cttcctcggc
      241 gcggcacaaa tggtcgacga actcgacctg ctgcccgatg ccgtcgccgc caccctgctt
      301 gccgacatcg gacgctacgt ccccgattgg aatctattgg tttccgaacg ctgcaacagc
      361 accgtcgccg agctggtcaa aggtgtggac gaagtgcaga aactcaccca cttcgcccgg
      421 gtggacagcc tcgccacgcc agaagaacgc gcccagcagg cagaaactat gcggaaaatg
      481 ctgctggcga tggttaccga catccgcgtc gtgttaatca aactggcgat gcgtacgcgc
      541 accctgcaat tcttaagcaa cgttcccgac aatcctgaaa agcgcgccgt tgctaaagaa
      601 accctcgaca tttttgcccc actcgccaac cgcttgggcg tgtggcaact caaatggcag
      661 ctcgaagatt tgggcttccg tcatcaagag ccggaaaaat accgcgaaat cgccctgctt
      721 ttggacgaaa aacgcaccga gcgcctcgaa tacatcgaaa acttcctcaa tatcctgcgt
      781 acggaactca aaaaatacaa tatccacttt gaagtcgccg gccgcccgaa acacatctac
      841 tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg acggcctctt tgacatccgc
      901 gccgtgcgga ttctggttga taccgtcccc gagtgttaca ccacgctggg cattgtccac
      961 agcctttggc agcctatccc cggcgagttc gacgactaca tcgccaaccc gaaaggcaac
     1021 ggctataaaa gtttgcacac cgtcatcgtc ggtcccgaag acaaaggtgt ggaagtgcaa
     1081 atccgcacct tcgatatgca ccaattcaac gaattcggtg tcgccgccca ctggcgttac
     1141 aaagaaggcg gcaaaggcga ttccgcctac gagcaaaaaa tcgcctggtt gcgccaactt
     1201 ttggactggc gcgaaaatat ggcggaaagc ggcaaggaag acctcgccgc cgccttcaaa
     1261 accgagcttt tcaacgacac gatttatgtc ctgaccccgc acggcaaagt cctctccctg
     1321 cccacgggcg cgacccccat cgacttcgcc tacgccctgc acagcagcat cggcgaccgt
     1381 tgccgcggtg cgaaagtcga agggcagatt gtgccgctgt ccaccccgct cgaaaacgga
     1441 cagcgcgtcg aaatcattac cgccaaagaa gggcatcctt ccgtcaactg gctttacgaa
     1501 ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg cctacatccg ccagcaaaac
     1561 gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca aacagcttgc caaactcacg
     1621 cccaaaccca acctgcaaga gcttgccgaa aatctcggct acaaaaagcc agaagacctc
     1681 tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca tccaaaaagc ctgcggcacg
     1741 ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca tcgtcaaaca gtccaaaatc
     1801 aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag acggtctgat gaccacgctt
     1861 gccaaatgct gcaaacccgc gccgcccgac gacattgtcg gcttcgttac ccgcgatcgc
     1921 ggcatttcgg tacaccgcaa aacctgcccc tctttccgac acctcgccga acacgcgccc
     1981 gaaaaagtac tggacgcaag ttgggcggcg ttgcaggaag gacaagtgtt cgccgtcgat
     2041 atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg acgtatccga cgcgctcgcc
     2101 cgccacaaac tcaacgttac cgccgtgcaa acccagtccc gcgacttgga agccagcatg
     2161 aggttcacgc tcgaagtcaa acaagttacc gacctcccgc gcgtcctcgc cagcctcggc
     2221 gacgtcaaag gcgtattgag cgttacccgg ctttaaatac aaaaatgccg tctgaaagcc
     2281 gtataacgct tcagacggca ttttgattga cggggtttgc tattttttgt tgcatagt
//
LOCUS       DAXHKX010000227         2314 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXHKX010000227 DAXHKX010000000
VERSION     DAXHKX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.227"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(59..1468)
                     /gene="dacB"
                     /locus_tag="ACFMWZ_002040"
     CDS             complement(59..1468)
                     /gene="dacB"
                     /locus_tag="ACFMWZ_002040"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC5935931.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1633..2199
                     /locus_tag="ACFMWZ_002041"
     CDS             1633..2199
                     /locus_tag="ACFMWZ_002041"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC5935932.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          530 a          565 c          663 g          556 t
ORIGIN      
        1 agtttgtcct agttatctag gacagcccct taaatttaat ccactatatt ttcctgtttc
       61 aggctcggcg ttctttgcac atcagtttcg catccagcca gccatcgccg ccggagatga
      121 tgttgttggc aacgaagttg tccaagtctg gcagcaggga aacggcgcgg ccgctgttga
      181 tgatgacgac caccgccatc ggtttgtcgc ccagccaata acctgcaagg gcgcggacat
      241 tgttgagcgt gccggttttt aagcgcaaca gcccgccgct ttgtttgaag cggttgcgta
      301 aagtcccgtc tgtgccggcg atgggcagcg tatcgatgaa atcttgtgca aacgggctga
      361 aataagccgt ttccaacatt tgcgccatca ttctcgccgt taccctttct tttctggaca
      421 gacctgaacc gttttccaaa accaaatccg caacatcgat gcccgacacg gcaagttcac
      481 gccggacggc agacgctgcc tgttcggaaa cggcgggcag tttgccgtcg ccgccgagtt
      541 tgaggaagac ggaacgcgca attagattgt ccgaacgctt gttcatgtcc gtcaaaattt
      601 ccttcatcgg ctttgagtgt gcaacggcaa gcgtctgcgc gccttccggc gtgtcggata
      661 tgccgatgcc gtctgaaatc cgtccgccgc cgagcagcca gtggttggta aaactttgcc
      721 ggatcagttc gtcaagcgcg aacatccgga caccgacagg cttgcccaaa cagctctcgg
      781 gaatattgcc gcgcaatttc agcgtattgt ccgaaaaaga tgcacgcatc agttttttga
      841 tcgaagggca ggcagcttgg gaggcggtaa ttttcaagtt gttttgggcg aaaatatgcg
      901 gcaaaggcgg atcggtgagg atgtcggtac tgtcggcggc attgcgttcg gcgcgcacca
      961 taaccatacc ggcagacagc atagttggat tggggggcgt cataaacggc gaaccgctgt
     1021 cggcttcgaa atcgtcgggg ctgccgactt cgccccacag gctgtggtcg agcatcaggt
     1081 gtcccgtgat attgcgtatg ccttgttcgc gcaactggcg ttggacggca agcaggtttt
     1141 cctgattgaa aacggggtcg ccgctgcccg cccaatacag gtttccgtca agcgtgccgt
     1201 cgtttaccgt accgttgctt ttaaactcgg tcgcccagcg gtaattgctg ccgaaggttt
     1261 tgaaggcggc aaacgcggta acgagtttca ttgtggaggc ggggttgacg gggacatccg
     1321 agcggtggtc aatgatgact tttccgctgt caagctcttg gacatatacg gcgatttcgt
     1381 tttgcggaat gcgacccgta tcgagcgcgt gtgcggcgag ggaggcgaga agcagcagca
     1441 gggaggccgc tgttttgggg aaattcatag gtaaatctgt tacataaaaa aagcaattat
     1501 aaggcaaagc cggataagtg ggaacgctgt ggcggcgggc cggcttgttt gcaggggaaa
     1561 tcacatatat aataatcgct atcattatga aatgattgag acgcacaaac ttaatattca
     1621 ggaggaatga ttatggctaa gaaaatcagt attttggtgg gcagcctgcg ccgtgcttct
     1681 tttgcgcgca aagtggcatt gaatgcggcg gagatgttcc ccgaaggctg gcaggcggaa
     1741 atcgttgaaa tcggacatct gccgctttac aatttcgatt atgacgaccc tgcggtggaa
     1801 gatgtgccgc tacccgaaag ctacacggct ttccgcgaaa cgattaaggc ttcggacggc
     1861 attttgttcg ttacgtccga aaataaccgc accattcccg cctgtttgaa aaatgcggtg
     1921 gacatcggct cgaaaccgaa tgccgacgtg gcgtggaaaa acaaaccggc aggcatcatc
     1981 agccattccg tcggcaagat gggcggttac agttcccaaa aaaacctgcg ccttgcgctg
     2041 tcttatttcg atatgcccgt aaccggacag ccggaagtgt ttttgggcaa ttcgccgacg
     2101 ctgtttgatg aaaacggcaa attgattgac tcggcaaggg attttgttca gtcatatatt
     2161 aatcagtttg tcggtttgat tgaaagaaat gccaaataaa caaaccaagt cgaaaggccg
     2221 caaaaccggt tcgggttttg cggctttttt atatgggctg ggcagcgtca aggtttgccg
     2281 tttgccggat aatgcgcggc aaatgaccgg cgtg
//
LOCUS       DAXHKX010000228         2302 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXHKX010000228 DAXHKX010000000
VERSION     DAXHKX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.228"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..290)
                     /locus_tag="ACFMWZ_002042"
     CDS             complement(<1..290)
                     /locus_tag="ACFMWZ_002042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935933.1"
                     /translation="MAGLFVKDIHRVSFSVETPTFRAVESEFIWEGRNPFQIRATHRA
                     VLYVSSCVLKHFSDFSVETQAGDFSRLPKMPSEKRFGASLRCLRYSGLTKTST"
     gene            261..2141
                     /locus_tag="ACFMWZ_002043"
     CDS             261..2141
                     /locus_tag="ACFMWZ_002043"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HFC5935934.1"
                     /translation="MNIFYEESGQFKVAAVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELGKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGIKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGTYRPSISIYFDVDNE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          624 a          679 c          545 g          454 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaaccttttt
       61 tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac agaaaaatcc
      121 gaaaaatgtt tcaatacaca ggacgacaca taaagcaccg ccctatgtgt tgccctgatt
      181 tggaaggggt tgcgcccctc ccaaataaat tctgattcta ccgccctaaa ggttggggtt
      241 tcaaccgaaa aggaaacacg atgaatatct tttacgaaga gtccggccaa ttcaaagttg
      301 ccgccgtcgt ccaaaaaaac gacgccacct accaagtcga tacccaacac ggcaaacgca
      361 ccaaagtgaa ggcaaacaac gtctttgccg agtttgacgg cgatatggcg gcgtttttgg
      421 aaaacgcgca ggcacaggcg gcggacatcg acaccgattt attgtgggaa gtatgcggcg
      481 aagaggaatt taccgccgaa gccatcgccg aagaatatta cggtcatgcg ccgaccaaaa
      541 ccgagctggc ggcgactttg attgcgcttt acgccgcgcc gatgtatttc tacaaaaaag
      601 ctaaaggcgt gttcaaagcc gcgcccgaag aaactttgaa acaggcgctt gccgccatcg
      661 aacgcaaaaa gcagcaagac gcgcaaatcg acgcttgggc agaagccttg aaacgcggcg
      721 agatgccgtc tgaaatcgcg gcggatttga aaaccatcct gcacgcgccc gacaagcagt
      781 cgctgaccta caaagccttt accaaagccg ccgacgcgct gaaaacctct gcctacgaac
      841 tggggaaaaa aacgggcggc attacgtcca ttccccaata cctgcaagac gggtttgaaa
      901 tcaaatattt ccccaaagga acaggcttcc ccgaccttcc gcttcctgaa atgcctgact
      961 tacccaaagc cgacgttacc gccttttcca ttgacgacga atcgactacc gaagtggacg
     1021 acgctttaag cctgaccgat ttgggcaatg gcataaaacg cgtcggcatc cacattgccg
     1081 cgccgtcgct tgccattaag caaggcgaca aaatggaaaa aaacatcatg gaacgcttaa
     1141 gcacggttta ttttcccggc ggcaaaatca cgatgttgcc cgaaaactgg attgccgcgt
     1201 tcagccttga tgcaggtaca taccgccctt ccatcagcat ttatttcgat gtggacaacg
     1261 agttcaacgt cggcgcgccg acctgcaaaa tcgaagcggt caacatcgct gaaaacctgc
     1321 gtatccaagc catcgaaccg catttcaacg ccgaagcggg cttggacgaa gcgggcgaaa
     1381 tgatgttcgc ccaccatcaa gacctgattt ggttttatca attcgccacc gccctgcaaa
     1441 aagcgcgcgg caaatacgaa cccgaccgcg cgccgcaata cgattacagc atcgaattgg
     1501 ataaagaaga caacgtatcc gtcgtccgcc gcgaacgcgg ttcgcccatc gatacgctgg
     1561 tcagcgagat gatgattctt gccaacagca cttgggcgca aatgctccat gacaacgacc
     1621 tgcccggcct cttccgcgtc caacccaccg gaaaagtacg catgagcacc aaatccgagc
     1681 cgcatatcgg catgggcgtg cggcattacg gctggtttac ctcgccgctg cgccgcgccg
     1741 ccgactacat caaccaaaag cagctgatca gcctgataga cgacactgcc gagccgctgt
     1801 atcaaaacag cgatgccgag cttttcgccg cactgcgcga ctttgatgcc gcctataccg
     1861 cctacgccga cttccaacgc caaatggaag cctactggag cctggtttac ctgcaacagc
     1921 aaggcacgag cgagctgacg gcgacgattc tgaaagaaga cctcgtccgc atcgaaggtc
     1981 tgccgctagt aacacgcgcg accggtattc cgtttgacgc gctgcccaaa tcgcaggcat
     2041 tgttcaaaat caccgaatta gatgccgaga agcagtttgt ctcgctcaac tacatcaagg
     2101 cagtcgcacc cgcgggtaca acggcaggca atgccgtctg aagcccgata cggcaaactc
     2161 cggcaaaaac agaaacctga atctcatcat tcccacagtc gggaatccgg ttttttgggt
     2221 ttccgttgtt tttccgtttc aatgaacttc caagccgccg ttcccgataa atacccgcaa
     2281 tctaaaatcc cgtcattccc ac
//
LOCUS       DAXHKX010000229         2288 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXHKX010000229 DAXHKX010000000
VERSION     DAXHKX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.229"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            21..749
                     /gene="rpe"
                     /locus_tag="ACFMWZ_002044"
     CDS             21..749
                     /gene="rpe"
                     /locus_tag="ACFMWZ_002044"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC5935935.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(772..1803)
                     /gene="ruvB"
                     /locus_tag="ACFMWZ_002045"
     CDS             complement(772..1803)
                     /gene="ruvB"
                     /locus_tag="ACFMWZ_002045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC5935936.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1885..2193)
                     /locus_tag="ACFMWZ_002046"
     CDS             complement(1885..2193)
                     /locus_tag="ACFMWZ_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935937.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          464 a          550 c          733 g          541 t
ORIGIN      
        1 ggatattgaa ggaaaaaaac atgactactt accgtatcgc acccagcatt ttatcggcag
       61 attttgcacg gcttggcgaa gaggtggaaa gcgtcattgc ggcaggtgca gatttgatcc
      121 attttgacgt gatggacaac cattatgtgc cgaacctgac cttcggccct atggtttgcg
      181 cggcgttgaa gccttatgca agcgtgccga tcgatgtgca tctgatggtc gaacccgttg
      241 acgacctgat tcagtcgttt gccaaagcag gagcatcaat catcacgttc catcccgaag
      301 cgagccgcca tatcgaccgc agcttgagcc tgattcggga catgggctgt caggcggggc
      361 tggtgttgaa tccggcaacg cccgtatatc tgttggaaaa cgtattggac aggctggata
      421 tggttttgct gatgtcggtc aaccccggat tcggcggaca aagcttcatc ccgcacaccc
      481 ttgaaaaaat ccgccgggtg cgggcgatgc tggatcggta cgaagcacaa agcgggcggc
      541 acatcgccat cgaagtggac ggcggcatca aaaccgacaa tattgccgcc gttgcccgag
      601 ctggtgcgga tacctttgtt gccggttctg cgattttcgg caaaccagac tataaggcgg
      661 tgattgatgc gatgagggcg gaactggaaa aaagcggcag gctgaacctt gcgccaatga
      721 ttgaaataga aatgccgtct gaaaattagc tctgtttcag acggcattgc gttatttttc
      781 gacgggcagc ccgaaatgca ggtaggcgcg ttcggtcgcc atcctgccgc gcggggtgcg
      841 ttgcaggaag ccttgttgga tgagataggg ttcgataacg tcttcgatgg tgtctgttga
      901 ttcgccgatg gcggcggcaa cattgtccaa accgaccggg ccgccgccga atttgtgcaa
      961 aacggcttcg aggaatttcc tgtccatcac gtccagcccc tgcacgtcca cgtccagcat
     1021 acttaaagcg gcatcggcga tgccgccgtc gattgtgccg ttgtttttca cgtcggcgaa
     1081 atcgcgcacg cggcgcaaca ggcggttggc gatgcgcggc gtaccgcggc tgcgtttggc
     1141 gatttcttcc gcgccttctt cggacatatc gagttgcagg agttgtgccg aacggctgac
     1201 gatggtggca aggtcgcggt tttcgtaaaa ctcgaggcgg gagacgatgc cgaagcggtc
     1261 gcgcaacgga ttggtcagca taccggcgcg ggtggtcgcg ccgacaagcg tgaagggcgg
     1321 caggtcgatt ttaacggaac gggcggcggg cccttcgcct atcataatgt cgagccggta
     1381 gtcttcaagc gcggggtaga ggatttcttc gacaacaggg ctgaggcggt ggatttcgtc
     1441 gataaacaat acgtcgtgcg gatcaaggtt ggtcaaaagg gcggcgagat cgccggcgcg
     1501 ttcgaggacg gggccgctgg tttggcgcaa atttacgccc aattctttgg cgatgatgtg
     1561 cgccagtgtg gttttaccca gcccgggcgg accgaaaagc aagacgtggt cgagtgcttc
     1621 gccgcgcttt ttggcggctt ggatgaaaat ggcgagctgt tctttggctt tatgctgccc
     1681 gatatagtcg tccagcgttt tggggcggag ggcgcgttcg agcagttctt cctgtgcgga
     1741 ggcggtttgg gcggcaacaa tgcgttgcgg ttgagcggca gtcaggttgt cggtttgcag
     1801 catagtgttc cctttgtcgg gtatgccgtc tgaacggtcg gcggcgtttc agacggcatt
     1861 gaaaagataa cggtcaaaag cgttttaccc gcgcgtttcg cgccaaacga gatagtcgcg
     1921 caggggcggc ataggcgggt tgatgcagtg tgggcagatg cctgtggtgt cttcgtcgtc
     1981 gctgtcgatg tgataggcgt tggggtcgaa gcggacttgc tgcatcaccg cctgcgccaa
     2041 cgcctgcgcc tgaacaacgt cgaaattgaa tttttcgagg atttcgttca ataggcggat
     2101 ttcgccaccg ctaaattccg cactgtgttt gaggatgagg cgttgataat cttcgttgtt
     2161 gattttaggc aataaatcgg ttttcagggt catgatttta tgtcgggggc ggaatggctg
     2221 aagcgtgaat tatagcggat atggcggcgg ctgttgcaaa gcttggattg ggtatcgtcc
     2281 tcaaaact
//
LOCUS       DAXHKX010000230         2283 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXHKX010000230 DAXHKX010000000
VERSION     DAXHKX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.230"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(3..590)
                     /locus_tag="ACFMWZ_002047"
     CDS             complement(3..590)
                     /locus_tag="ACFMWZ_002047"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC5935938.1"
                     /translation="MEHKLPQLPYEPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            752..2158
                     /gene="dnaB"
                     /locus_tag="ACFMWZ_002048"
     CDS             752..2158
                     /gene="dnaB"
                     /locus_tag="ACFMWZ_002048"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC5935939.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          556 a          587 c          612 g          528 t
ORIGIN      
        1 aatcaggaca aggcggcgaa gcgtttggcg acttcgtccc agttgacgat ttcccaaaaa
       61 cctttcaggt agttgggacg gctgttgcgg tagtcgatgt aataggcgtg ttcccacacg
      121 tcgcaggtca gcagcggcgt gttttcagtg gtcagcggcg tagcggcgtt ggaagtggaa
      181 accaaatcca atccgccggc aggggttttt accagccacg cccaaccgga gccgaaagta
      241 ccggccgcgc aggcattgaa cgcttcttgg aatttctcga agctgcccca tttcgcgtcg
      301 atggcggcgg ccagttcgcc ggcaggtttg ccttgacctt tggacgtgaa acccagccag
      361 tagaaggtgt ggttccaagt ttgcgccgcg ttgttgaaca cgccgcccga agattttttc
      421 acaatctctt ccaaaggtag gttttcaaat tcggtacctt tgatttgatt gttcaggttg
      481 gtgatgtagg tttgatggtg tttgccgtag tggaattcca aagtctcttt gctcagatgc
      541 ggggacaatg cgtccggttc ataaggcagt tgcggcagct tatgttccat tttgtgctcc
      601 taatattgtt ttaaatgttg tattttggca gtgtgctgca aatagctcag cagcgcacat
      661 attctacctg ttttgaggga tggaaaccaa ttaaacctgc tttacgctat aatagaagat
      721 tgcaatttcg gcacgacaga taggatgtac catgaacgat tacacagcta tgccgtctga
      781 agaccgtgag gtcggcgcat tgtcgctgcc tccacattcg atggaggcgg aacaatccgt
      841 tttgggcggg ttgatgctgg aaaatccggc atgggacagg attgccgatg tggtttcggg
      901 agaggatttc taccggcatg aacaccgcct gattttccga tccattgcca aattaatcaa
      961 tgagagccgt cccgccgatg tgattacggt tcaggaagat ttgcagcgga acgaagaatt
     1021 ggaagcggca ggcggatttg aatatctgat tacgctggcg caaaacaccc cgtctgccgc
     1081 caacatccgc cgctacgccg aaatcgtgcg cgagcgttcc attatgcgcc aactcgccga
     1141 agtggggacg gaaatcgccc gcagcgcata caatccgcaa ggcagggacg cgggtcagct
     1201 tttggacgag gcggaaaaca aagtattcca aatcgccgaa agcacggcca aatccaagca
     1261 gggctttttg gagatgcccg atttgctgaa agaagtcgta cagcgcatcg atatgctcta
     1321 ctcgcgcgac aatcccgatg aagttaccgg cgtgccgacg gggttcatcg acctcgacaa
     1381 aaaaacctcg ggtctgcaac ccggcgacct gattatcgtt gccggtcgtc cgtctatggg
     1441 taagaccgcc ttttctatca atatcgccga acacgttgcc gtagaaggca agctgccggt
     1501 tgccgtcttc tcaatggaga tgggcggggc gcaactggtc atgcgtatgc tcggctcggt
     1561 cggacgcttg gatcaaagcg tcctgaaaac cggcaggctc gaagacgaac actggggtcg
     1621 tctgaacgaa gcagtcgtca aactctccga cgcgcccgtg tacatcgacg agaccccggg
     1681 tttgactgcg ctcgaactgc gcgcccgcgc ccgtcgtctc gcccgtcaat ttaacaataa
     1741 gcttggattg atcgtcatcg actacctgca actgatggcg ggatccggcc gttccgacaa
     1801 ccgagcttca gagctgggcg aaatttcacg ttcgctcaaa gcgttggcga aagaattgca
     1861 agtccccatc atcgccctgt cgcaattgag ccgtaccgtc gaatcgcgta ccgacaaacg
     1921 cccgatgatg tccgacctcc gcgaatccgg tgctatcgaa caagatgccg acctgattat
     1981 gttcatgtac cgcgacgaat actacaacca ggactcaccc atgaaaggcc ttgccgaatg
     2041 tatcatcggc aaacaccgca acggtcccgt cggtaaaatc ttcctcacat ggacgggaca
     2101 attcaccaaa ttcgacaatg ctgcctatat tcccgaggag gcaaagatag aagattaaat
     2161 ggctatataa aaatttatta ggcgaaatca ggcaaaatcg tttaaaatca tgctgagaga
     2221 ttgccctaaa aaataaaacg cggtcttgag gcatttttgc attcagcccg catataattg
     2281 aaa
//
LOCUS       DAXHKX010000231         2219 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXHKX010000231 DAXHKX010000000
VERSION     DAXHKX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.231"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            107..1918
                     /gene="typA"
                     /locus_tag="ACFMWZ_002049"
     CDS             107..1918
                     /gene="typA"
                     /locus_tag="ACFMWZ_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC5935940.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          566 a          631 c          578 g          444 t
ORIGIN      
        1 tctgaaaggc aagggcttca gacggcatct tcatttccca aatactgtcc ggtaaagcgt
       61 ggtataatgc gtagttatct cttacagttt ttgaaaaaca ttaattatga aacaaatccg
      121 caacatcgcc atcatcgccc acgtcgacca cggcaaaacc acattggtcg accaactgct
      181 gcgccaatcc ggcacattcc gcgccaacca gcaggttgac gagcgcgtga tggacagcaa
      241 cgacctcgaa aaagaacgcg gcatcaccat cctcgccaaa aacaccgcca tcgattacga
      301 aggctaccac atcaatatcg tcgacacgcc gggacacgcc gacttcggcg gcgaagtaga
      361 gcgcgttttg gggatggtgg actgcgtcgt cttgttggtg gacgcgcagg aaggcccgat
      421 gccgcaaacc cgtttcgtga ccaaaaaagc cttggctttg gggctgaaac cgattgtcgt
      481 catcaataaa atcgacaaac cgtccgcccg tccgagctgg gtcatcgacc aaactttcga
      541 gctgttcgac aacttgggcg cgaccgacga gcagttggat ttcccgattg tttatgcttc
      601 cggtctgtcc ggtttcgcca aattggaaga aaccgacgag agcaacgaca tgcgtccgct
      661 gttcgatact atcttaaaat atacgcctgc accgagcggc agcgcggacg aaacgctgca
      721 actgcaaatt tcccaactcg actacgacaa ctacaccggc cgcctcggta tcggtcgtat
      781 cttgaacgga cgaatcaaac ccggccaaac cgttgccgtc atgaaccacg atcagcaaat
      841 cgcccaaggc cgcatcaacc agcttttggg tttcaaaggt ttggaacgcg tgccgcttga
      901 agaagccgaa gccggcgaca tcgtgattat ttccggtatc gaagacatcg gtatcggcgt
      961 aaccatcacc gacaaagaca atcccaaagg cctaccgatg ttgagcgtgg acgaaccgac
     1021 gctgacgatg gactttatgg tcaacaccag cccgctggcg ggtacggaag gcaaattcgt
     1081 aaccagccgc caaatccgcg accgcctgca aaaagaattg ctgaccaacg tcgccctgcg
     1141 cgtggaagat accgccgatg ccgacgtgtt ccgcgtatcc gggcgcggcg agctgcacct
     1201 gaccattttg ctggaaaaca tgcgccgcga aggctacgaa ctcgccgtcg gcaaaccgcg
     1261 cgtcgtgtac cgcgacatcg acggtcaaaa atgcgaaccg tatgaaaacc tgaccgtgga
     1321 tgtacccgac gacaaccaag gcgcggtaat ggaagaactc ggccgccgcc gtggcgaact
     1381 gactaatatg gaaagcgacg gcaacggacg cacccgcctc gaataccata ttccagcgcg
     1441 cggcttgatc ggtttccaag gcgaatttat gaccctgacg cgcggggtcg ggctgatgag
     1501 ccatgtgttc gacgattacg cgcccgtcaa acccgatatg cccggccgcc acaacggcgt
     1561 gctggtgtcc caagagcagg gcgaggcagt cgcttacgcc ttgtggaatc tggaagaccg
     1621 cggtcgtatg ttcgtatcac ccaacgacaa aatctacgaa ggtatgatta tcggcatcca
     1681 cagccgcgac aacgatttgg tggtcaaccc gctcaaaggc aaaaaactta ccaacatccg
     1741 cgccagcggc accgacgaag ccgtgcgcct gaccacgccg atcaagctga cgctggaagg
     1801 tgcggttgag tttatcgacg atgacgaact cgttgaaatc acgccgcaat ccatccgtct
     1861 gcgcaagcgt tacttgagcg aattggaacg ccgccgccac tttaaaaagc tggattgatg
     1921 tttactggat taatgtttaa atgatacgcg atgccgtctg aaaaatttca gacggcattt
     1981 tttattcgga cgggctttgc ggcttcttga agctgtttca gacggcgttt ttcctaccca
     2041 atcaagaaac tgccgccatt tttccagcgg tatatcgccc cgtcgcgttt cggtatcggg
     2101 ttcggcttcc cggcagcatg tcgaacaatg ccgtttgaag gaatacgcct tttgagtcct
     2161 tacaagctta ggaagaggat atagtggatt aacaaaaatc aggacaaggc gacgaagcc
//
LOCUS       DAXHKX010000232         2199 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXHKX010000232 DAXHKX010000000
VERSION     DAXHKX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.232"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(161..592)
                     /locus_tag="ACFMWZ_002050"
     CDS             complement(161..592)
                     /locus_tag="ACFMWZ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935941.1"
                     /translation="MMFFQIACNSENSYISLLKSMRFYIDNKECDYIFFRKAVNISDD
                     FIQSGFITPEGLITKNSNPKLFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDA
                     YIEFAYSEDFYVLPEIEIDKRTLKSLNFYIDFGVKYIINKI"
     gene            complement(666..1109)
                     /locus_tag="ACFMWZ_002051"
     CDS             complement(666..1109)
                     /locus_tag="ACFMWZ_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935942.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1272..1544)
                     /locus_tag="ACFMWZ_002052"
     CDS             complement(1272..1544)
                     /locus_tag="ACFMWZ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935943.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
BASE COUNT          717 a          445 c          303 g          734 t
ORIGIN      
        1 atccactata tatatccatt catatagaga aaaattctta tacccaatat aaaacaaggt
       61 ctttcattat attttgaaaa acctagaagc tttgtatcta ttggtttatc aaaattaata
      121 attattgcag aagacatgta ggctaataat cttgatttat ctatatttta ttaattatat
      181 atttaacccc gaagtcaata tagaaattta gtgattttaa agttctctta tcaatttcaa
      241 tctcagggag tacataaaaa tcctcagaat aggcaaactc tatatatgca tcatcattcg
      301 ataataattc aataatattt ttcatttcat cagaaagaaa tgtaaccatc tcatattgac
      361 atttattttt agataccatt tttgaatatt gattaaataa ttttggatta ctatttttag
      421 taattaatcc ttccggagta ataaaaccac tttgtataaa gtcatcactt atattaacag
      481 ccttccgaaa aaaaatataa tcacactctt tgttatcaat ataaaatctc atactcttta
      541 ataatgaaat ataagaattt tcagaattac aagcaatttg aaaaaacatc attactccta
      601 aaactcataa atagtaagga atatatatat atgcatattc cttactattt tttcacaaat
      661 tatgcttatc tatgcttatt tccataaaaa gcatgccctc gaattttttg acttgagttc
      721 tggaaatgag aataatttga atggatttcc ttctgtccat gtctaataac attttgtctt
      781 ccccaagctt ggtttgctac cgctcttgct tgttgagatg ttcgtgcata gacattagct
      841 cctcttgata aagcttgtgc tgcaacacgc tgcgtaaccc aacgctgcgc aaggaatctg
      901 cccgcatgca aaattcctat tgccaccaac ggtgccgcag ccccttcagt ttctttcatc
      961 tccttttgag aaagctccgc caactgcatc ggcgcatctg cccgcgtgtg gaacacttgg
     1021 tcttcaaatg cctgattgtc caagccgttt gccattgcgg gggcaatcat agacagcatc
     1081 attacggctg cggtgatttg ttttttcata ataactcctt tggattacaa ggttggaaaa
     1141 tcaaagccct gcttagaacg tatgttgcac acccaatttc aataggtaaa tcagattgca
     1201 aatccagcaa tttgaatatt gtcattgttc cgtgcaaaag ggatttttat tgatgagttg
     1261 tgtactgggt ttcagcttgg ttttttagat aatcttttca caagaacata catcaatact
     1321 gtccaaaaaa aaagatttga taaatatatt gcaacatatt ttattccacc tccatccgaa
     1381 acagaaacca atatattttt aattaataca tagctgatta ttacatttat tattcctata
     1441 atatataagg atcttgccaa aatttttttt gattttattt taggaatatc tcttatccat
     1501 gctaaaatac agcccaatgt cgaaaagaaa ataacagcaa acactacctt acctcctctc
     1561 acttccaacc aaaccgatag caggtttggt tggaagttgg tttatttatt atttaacggc
     1621 gaatgtcagt gttcttaccc gtagaacctg cataaccatt tatgccacca gcaacagccc
     1681 ccactgctac acctcgcata ccattaatca gaattcttcc tattttttcg aatccactat
     1741 ttcttccaaa gatgcagcaa acgcaggttg agcctagacc caatctacgt ttgctgccgc
     1801 atctgatccc tattgtttct tatccttaca tcttcctgcc ttgtcaatca aataaagaca
     1861 gaagactata caaaaactga ccgacatact gaaaatacct atccccttcc atgcaatctc
     1921 cgcaccattg acccaataga ttagactgag aaacaacaaa gccacagtat aaatcaacgt
     1981 aaaaaatatt gcgtaaagca caataaaggg aacttttatc tcacggttgt tttttataat
     2041 atattcagca acttgatttc cgaatatacc tgataaaaaa tatagtatta gatcatccat
     2101 ttcgtttatc ttctatgttt tcccattgcg gcgctagaag actgatttaa tgctgcacca
     2161 tgcaatatag tgaattaaca aaaaccagta cggcgttgc
//
LOCUS       DAXHKX010000233         2161 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXHKX010000233 DAXHKX010000000
VERSION     DAXHKX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.233"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            373..885
                     /locus_tag="ACFMWZ_002053"
     CDS             373..885
                     /locus_tag="ACFMWZ_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC5935944.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            965..2044
                     /gene="aroB"
                     /locus_tag="ACFMWZ_002054"
     CDS             965..2044
                     /gene="aroB"
                     /locus_tag="ACFMWZ_002054"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC5935945.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          526 a          680 c          527 g          428 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gccaaggcga ggcaacgctg tactggtttt
       61 tgttaatcca ctatagtatt gataaacata ttatcttcaa tatattcaat tggataattg
      121 tttacctaag caaagataat tgccttttcc tgacaaataa gtgaaatcaa cggattgtca
      181 aaacacagcc tgaaataaaa aacctccctg atttctttta tttgtcctta aaatcagaaa
      241 ggttcgggat ggtcgggtta tttttccaaa cgtaccgccg ccctgccgat ttcgtataaa
      301 attccgccgt aacccgacaa gcccgaaccc tgtcgccccg aaaggcgggg tgtcaaacat
      361 taaggaattg tgatgaaaaa ctttaacggc aaactcatcc tcatcggact gatgggcgcg
      421 ggcaaaacca cgctgggccg gcaaatggcg cagcggctgg attaccgttt ttacgacagc
      481 gatcacgaaa tcgccgtagc cgccggcgtt cccatcccca ccatatttga aatggaaggc
      541 gaacagggat tccgttcgcg cgaaaccgcc atactcaaaa agctggttat cctgccccat
      601 atcgtcctgt ccaccggcgg cggcgcggtg ttaaaagaag aaaaccgcgc ccttatccgc
      661 aaaagcggca cggtcgtcta tctgcacgcc ccgcccgaaa ccctgctcga acgcacgcgc
      721 tgcgacaaca gccgtccttt gctgcaagtt gccgatcctt tggcgaaatt acgtgaactc
      781 tacgccgcac gcgaccccgt ttaccgccaa accgccgact ttaccgtaga atcggcaaac
      841 tgccgggaaa ccgtgcaaac cctgctcaaa cgcttatccc gataaaccgg catatgcgcc
      901 gcgcccagaa aaccaagccg cgccccgccc ggcgggccgg cggttcaaac tttaaggaac
      961 aacaatgaaa acactgaccg tacacacccc gtcccacagc taccccatct ttatcggcaa
     1021 cggactgctg ccgcaggcag gaagcctgct caaaccgcat ttgggcaaac gcgccgccat
     1081 catcgccaac gaaaccgtcg ccccgctcta cctcggcacg cttcagacgg cattggatgc
     1141 ggcaggcgta tcccatttca gcatcatcct gcccgacggc gaggcgcaca aaaactggca
     1201 gacgctcaac ctcatctttg acgggctgat gcaaaaccgc gccgaacgca aaaccacgct
     1261 catcgcactg ggcggcggcg tgatcggcga catggtcggc tttgccgctg ccacctacca
     1321 gcgcggcgca ccgttcgttc aaataccgac cacactgttg agtcaggtcg actcatcggt
     1381 gggcggcaaa accgccatca accacccgct cggcaaaaat atgatcggcg cgttttacca
     1441 gccgcaggcg gtgcttgccg acttggacac gctgcacacc ctgcccgccc gcgaactgtc
     1501 cgccggtatg gcggaagtca tcaaatacgg cgcgctcggc gacatcggtt ttttcgaatg
     1561 gctcgaacaa aacatatccg gactgatgac gctcgatcgg gaaaaactcg cccaagccgt
     1621 gtaccgctgc tgccaaatga aggcagacat cgtcgcccaa gacgaaaccg aacagggcat
     1681 acgcgcatgg ctcaacctcg gacacacctt cggacacgcc attgaaaccg agatgggtta
     1741 cggcacttgg ctgcatggag aagccatcgc cgccggctgc gtgttggcgg cgcgtttgtc
     1801 cgaacaactg ggcaaaacct ccgccgcaga taccgcgcgg ctcgccgccc tgctcgaagc
     1861 cgccggactg ccgtccgcac cgcccgtgtt tgcctttgaa aaatggctcg aacatatgag
     1921 ccacgataaa aaagtcagcg gcggcatcat gcgctttatc ggtctgaacc ggctgggcga
     1981 agccaacatc accgaaatta ccgacacgga catcctccgc cgcaccctgc aaccgtatct
     2041 ctgatttcct ctgccgatgt gctgccgcgc gggtttgacg cacgatgatg attttccatc
     2101 atctttctcc gtaaaagcgg gaatccggtc cgttcagttt cggtcgtttc cgataaattc
     2161 c
//
LOCUS       DAXHKX010000234         2122 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXHKX010000234 DAXHKX010000000
VERSION     DAXHKX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.234"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            123..1508
                     /locus_tag="ACFMWZ_002055"
     CDS             123..1508
                     /locus_tag="ACFMWZ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC5935946.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1528..2046
                     /locus_tag="ACFMWZ_002056"
     CDS             1528..2046
                     /locus_tag="ACFMWZ_002056"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC5935947.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          443 a          648 c          574 g          457 t
ORIGIN      
        1 gttcaaataa acaggaatat aacttcagac aaacaactta ccgcccgatt tgtgctatcg
       61 ttttcgcaca acttaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acgtactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcgatgct cggacgtctg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga gcctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccgg ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgc ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttcgcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgcaacag
     1741 gcgcaaaacg gtgcgaacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggggtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca tt
//
LOCUS       DAXHKX010000235         2027 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXHKX010000235 DAXHKX010000000
VERSION     DAXHKX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.235"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..2024
                     /gene="pilC"
                     /locus_tag="ACFMWZ_002057"
     CDS             <1..2024
                     /gene="pilC"
                     /locus_tag="ACFMWZ_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC5935948.1"
                     /translation="TYPDSSSSRIFLQNLKRKNDPNKPGRYSLADLSALDIQSKEPTF
                     TGRQTIIRLDGGVRHIQLDKSNEATGLNGNDGKNETFGIVSEGSFMPDTSEWKKVLLP
                     WTVRDSADDSKFKAFNKEENNGNKPKYSQKYRIRDNGNNNNRNLGDIVNSPIVAVGEY
                     LATSANDGMVHIFKKGNGGDDRSYNLKLSYIPGTMPRKDIESKESTLAKELRAFAEKG
                     YVGDRYGVDGGFVLRKVERNGKDHVFMFGAMGFGGRGAYALDLTKIDSNNLTGVSMFD
                     VLNDKNNNKNDNNRVELGYTVGTPQIGKTHDGKYAAFLASGYATKTIDDQQNKTALYV
                     YDLESSGTLIKKIDVPGGKGGLSSPTLVDKDLDGIVDIAYAGDRGGNMYRFDLSSDNP
                     SSWTVRTIFSGNKPITSAPAISQLKDKRVVIFGTGSDLSEDDVDKTDEQYIYGIFDDD
                     TATTGPVNFSGTGGGLLEQVLTEENKTLFLTDYKRSDGSGSKGWVVKLKDGQRVTVKP
                     TVVLRTAFVTIRKYKDNGCGAETAILGINTADGGKLTKKSARPIVPADNTAVAQYSGH
                     KQTTKGKSIPIGCMEKDNGIVCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGI
                     DPDGKRSGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKRISWREIFY"
BASE COUNT          586 a          515 c          529 g          397 t
ORIGIN      
        1 ccacctaccc cgacagttcg agcagccgca tcttccttca aaatctgaaa agaaaaaacg
       61 accccaacaa acccggccgc tattccctcg cagacttgag tgcgttggat attcaaagta
      121 aagagccgac tttcacaggg cggcaaacaa tcatccgatt ggatggcggc gtacggcata
      181 tccaactgga taaaagcaat gaggccaccg gtttaaatgg aaatgacggc aaaaacgaaa
      241 ctttcggcat tgttagcgaa gggagcttca tgcctgatac cagcgagtgg aaaaaagtat
      301 tgctgccttg gacggttcgg gattctgctg atgacagtaa atttaaagca ttcaacaaag
      361 aagaaaacaa cggcaacaag ccaaaataca gtcaaaaata ccgcatccgc gacaacggca
      421 acaacaacaa tcgcaatttg ggcgacatcg tcaacagccc gattgtggcg gtcggcgagt
      481 atttggctac ttccgccaac gacgggatgg tgcatatctt caaaaaaggc aacggcgggg
      541 atgaccgcag ctacaatctg aagctcagct acatccccgg cacgatgccg cgcaaggata
      601 ttgaaagcaa agaatccacc ctcgccaaag agctgcgcgc ctttgccgaa aaaggctatg
      661 tgggcgaccg ctacggcgtg gacggcggct ttgtcttgcg caaagtcgaa cggaacggga
      721 aagaccatgt gtttatgttc ggcgcgatgg gctttggcgg cagaggcgcg tatgccttgg
      781 atttgaccaa aatcgacagc aataacctga ccggcgtttc catgtttgat gtcctaaacg
      841 acaaaaataa caataagaat gacaataatc gcgtggaatt aggctacacc gtcggcacac
      901 cgcaaatcgg caaaacccac gacggcaaat acgccgcttt cctcgcctcc ggttatgcga
      961 ctaaaaccat tgacgaccaa caaaataaaa ccgcgctgta tgtgtatgat ttggaaagca
     1021 gcggtacgct gattaaaaaa atcgatgtac ccggcggcaa gggcgggctt tcgtccccca
     1081 cgctggtgga taaagatttg gacggcattg tcgatatcgc ctatgccggc gatcgcggcg
     1141 ggaatatgta ccgctttgat ttgagcagcg acaatccgtc cagctggact gtacgcacta
     1201 ttttcagcgg caacaaaccg attacttccg cgcccgccat ttcccaactg aaagacaaac
     1261 gcgtggttat cttcggcacg ggcagcgatt tgagtgagga tgatgttgat aaaacggacg
     1321 aacaatatat ttacggtatc tttgacgacg atacggcgac gacgggtcct gtaaacttca
     1381 gcggtacggg aggcgggctg ctcgagcaag tgcttactga ggaaaataaa accttattcc
     1441 tgaccgatta caagcgatcc gacggctcgg gcagcaaagg gtgggtagtg aaattgaagg
     1501 acggacagcg cgttaccgtc aaaccgaccg tggtattgcg taccgccttt gtaaccatcc
     1561 gcaaatataa agacaacggc tgcggcgcgg aaaccgccat tttgggcatc aataccgccg
     1621 acggcggcaa gctgaccaag aaaagcgcgc gcccgattgt gccggccgac aatacggctg
     1681 tcgcgcaata ttccggccat aagcaaacca ccaaaggcaa atccatccct ataggttgta
     1741 tggaaaaaga caatggaatc gtctgcccga acggatatgt ttacgacaaa ccggttaatg
     1801 tgcgttatct ggatgaaaag aaaacagacg gattttcaac aacggcagac ggcgatgcgg
     1861 gcggcagcgg tatagacccc gacggcaagc gttccggcaa aaacaaccgc tgcttctccc
     1921 aaaaaggggt gcgcaccctg ctgatgaacg atttggacag cttggacatt accggcccga
     1981 cgtgcggtat gaaacgaatc agctggcgtg aaatcttcta ctgattt
//
LOCUS       DAXHKX010000236         1945 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXHKX010000236 DAXHKX010000000
VERSION     DAXHKX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.236"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            245..1702
                     /locus_tag="ACFMWZ_002058"
     CDS             245..1702
                     /locus_tag="ACFMWZ_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFC5935949.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFIPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          441 a          585 c          440 g          479 t
ORIGIN      
        1 accgccctat cggataagcc cttaacagaa aaggctttac ccgcgccgta tcggaacaca
       61 tcctctaaaa tacaatccgt tgaattgaaa aaaatataaa aacatccgcc cgcgaaaaac
      121 ggcagcgcgt cgtttgacaa agaatgaaaa tatcggttaa aaaccgattt tcatacaaaa
      181 aacaccgctg ccgtccgcat ccgtttcaga cggtattgag agaaaatctt ttaggagaac
      241 ctttatgtcc cggcatcccg cccccaccgg agaaaaaaca ttcttcggcc accccttcca
      301 gctttccacc ctcttccata tcgaattgtg ggaacgtttt tcattttacg gaatgcaggg
      361 catcctgctg atttacctct actacaccgc cgacaaaggc ggcttgggca tagacaaaac
      421 cctcgccggc ggcattgtcg gcgcatacag cggcagcgtg tacctgtcca ccattttggg
      481 ggcgtggttt gccgaccgag tatggggtgc ggaaaaaacc ctcttcctct cgggcatcgt
      541 cgtgatgctc ggacacatcg tccttgccgc cgccccgggc ctgtacggcc ttttaatcgg
      601 gctgatattc atcgcattgg gcagcggcgg cgtgaaatct acggccagtt ctatggtagg
      661 cgcattatac gaacaggacg aaatgcgccc gctgcgcgat gcgggatttt ccattttcta
      721 catcgccatc aatatcggcg gcttcttagg ccccttattg accggactgc ttcaagaaaa
      781 catcggtttc cattatggtt tcggcgcggc ggcggtcggt atggcattcg gcttgtggcg
      841 ttattccttg ggacgtaaaa acctgcccca ccccaccgtc ccccatccgc tttcaaaagg
      901 acagggcaaa actgcggccg ccgtcggcat tgccctcatc gccgcacttg caaccgccat
      961 caaaacgggg cttgtcaacc tcgacaattt ctccggcatc ctcttatcta ccgtcatcct
     1021 tgccgtcatc gcctatttcg cccgcctgct gaccaacccc cgcgtcagtt ccgacaacaa
     1081 acggcacatc atcgcctaca tcccgctttt cctgaccatc tgtatgtttt gggccgtttg
     1141 gtttcagatt tacaccgtag caaccgtcta tttcgacgaa accgtcaacc gcaccatcgg
     1201 ttcgtttacc gtgcccgtcg cctggaaaga ttccatgcaa agcctgtggg tcatcctgtt
     1261 ttccggactt atggcggcaa tgtggacaaa aatggggcgc aaacagccca aaaccccgct
     1321 gaaattcgct atggcggtat ttgttaccgg cgcgtcgttt ttgggattca tcccctttat
     1381 ttccgccggc acgccgatgc ctattgcggt tttcgcactg atcgtcctcg ccatcacgat
     1441 aggcgaactg atgatttccc cgattgcgct gtccatctcc accaaaatcg caccgccttt
     1501 attcaaaacc caaatggtcg cccttaattt ccttgccttt tcattaggct tcactttggg
     1561 cggcgtattg tttgaaaaag gctatcaggc gggcgacgaa atcggcttct accggctgct
     1621 gttctacatc ggcgcagcca caggcttcct gctgctcctg ctcgtcccca aattgaacaa
     1681 aatgctcgaa ggcacagact aagtcccgcc ccgatgccgt ctgaaccctt cagacggcat
     1741 ttttccgcat aatgaaacca aaccgtttcc acccgacagg acaggctccc cccaaccgga
     1801 aggcagcctg ccgattgtca tttaaatatc tgactgattc cgtctgtttg caagggaaat
     1861 agccgtttat ttccgcttgg gcggaaaccg gttctattga gtaaaaggca ttttatccga
     1921 ctaaattagt gctgcatcaa cggaa
//
LOCUS       DAXHKX010000237         1877 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXHKX010000237 DAXHKX010000000
VERSION     DAXHKX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.237"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(102..386)
                     /locus_tag="ACFMWZ_002059"
     CDS             complement(102..386)
                     /locus_tag="ACFMWZ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935950.1"
                     /translation="MVSTKSLSPDRLFSVWLFSVWLFSVWLFSVWLFSVWLFSVWLFS
                     VSLFSVWLFSVWLFSVWLFSVSLFSVWLFPVFSLFYSMSKYAVIPAQAGI"
     gene            complement(397..1482)
                     /gene="porB"
                     /locus_tag="ACFMWZ_002060"
     CDS             complement(397..1482)
                     /gene="porB"
                     /locus_tag="ACFMWZ_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC5935951.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVEVSRVKDAGTYKA
                     QGGKSKTATQIADFGSKIGFKGQEDLGNGMKAIWQLEQKASIAGTNSGWGNRQSFIGL
                     KGGFGTVRAGNLNTVLKDSGDNVNAWESGSNTGDVLGLGTIGRVESREISVRYDSPVF
                     AGFSGSVQYVPRDNANDKDKYTHKQSSRESYHAGLKYENAGFFGQYAGSFAKYADLNK
                     DAERVAVNTPNAHAVKDYQVHRVVAGYDANDLYVSVAGQHEAAKNNMVGSTDIGKKHE
                     QTQVAATAAYRFGNVTPRVSYAHGFKAKVNGKKAARYQYDQVIVGVDYDFSKRTSALV
                     SAGWLQEGKDKNKTEKTASMVGLSHKF"
     gene            complement(1690..>1877)
                     /locus_tag="ACFMWZ_002061"
     CDS             complement(1690..>1877)
                     /locus_tag="ACFMWZ_002061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935952.1"
                     /translation="LIFVNPLSPAAGAGFASARSERNRVRVFAVFITPGLQCPPIPSQ
                     VSVVKMQILKILNNHML"
BASE COUNT          543 a          410 c          428 g          496 t
ORIGIN      
        1 taaaacggtt tcttgagatt ttacgttctg gattcccact ttcgtgggaa tgacgggatt
       61 aaagtttcaa aatttattct aaatagctga aactcaacgc actagattcc cgcctgcgcg
      121 ggaatgacgg catattttga cattgaataa aaaagactaa aaacaggaaa aagccaaaca
      181 gaaaacagcg aaacagaaaa aagccaaaca gaaaaaagcc aaacagaaaa cagccaaaca
      241 gaaaacagcg aaacagaaaa aagccaaaca gaaaaaagcc aaacagaaaa cagccaaaca
      301 gaaaacagcc aaacagaaaa cagccaaaca gaaaacagcc aaacagaaaa aagcctgtct
      361 ggcgacaggc tttttgttga taccaatctt ttcagattag aatttgtgac tcaagccaac
      421 catgctggca gttttttcag ttttgttttt gtctttgcct tcttgcaacc aaccggcaga
      481 aaccagagcg gaagtgcgtt tggagaagtc gtagtcgaca ccaacgataa cttggtcgta
      541 ttgataacgt gctgctttct taccattcac tttagctttg aagccgtggg cgtaagaaac
      601 gcgaggcgtt acgttgccaa aacggtaagc ggcagtagcg gcaacttgag tttgctcgtg
      661 ttttttacca atatcggtag aaccaaccat gttgttttta gcagcttcat gctgaccggc
      721 aacagaaacg tacagatcat tagcatcgta accggcaact acgcggtgta cttggtaatc
      781 cttaacagca tgggcatttg gagtatttac tgcaacacgt tctgcatcct tgttcaaatc
      841 agcatatttg gcaaaagagc ctgcgtattg accgaagaaa ccggcatttt cgtatttcag
      901 accggcgtgg taagactcac ggctggactg cttatgtgtg tatttgtcct tatcattcgc
      961 attgtcgcgc ggaacgtatt gtacgctgcc gctgaagcct gcaaatacag gggaatcgta
     1021 acgtacggag atttcacggc tttctacacg gccgatagta cccagtccca gtacatctcc
     1081 ggtgttagaa ccagattccc atgcattgac gttgtcgccg ctgtctttca atacggtgtt
     1141 cagattaccg gcgcggacgg taccgaagcc gcctttcaag ccgatgaagg actggcggtt
     1201 accccagccg ctgttagtgc cggcgatgga ggctttttgt tccaactgcc aaatggcttt
     1261 catgccgttg ccgaggtctt cttgaccttt gaaaccgatt ttagaaccga agtcggcaat
     1321 ttgggttgca gttttagatt ttccgccttg agctttatat gtaccagcat cttttacgcg
     1381 agaaacttct acgcctgctt taattgtacc gtacaaggta acgtcagcca ttgctgcaac
     1441 aggcagggca gccagtgtca gggcaatcag ggattttttc attgctgtat tccttttcgt
     1501 agttaagaaa tttaagcagg ccgagctttc caagccgctt agctttgcat ttaccgccga
     1561 cgttttcggc gattccatgt ctgaaactat aaatgttcct gtctgaaaaa acaaacttct
     1621 gcctgttttt gtgtggaatc cgatgtgcat tttgaaggac ggatagagat attatgggta
     1681 ttttttgttt tataacatat ggttatttaa aatttttaag atttgcattt ttacaacact
     1741 tacttgggaa ggtattggag ggcattgcaa accgggggtt ataaagacag caaaaacgcg
     1801 cacgcggttt ctttcggaac gggcggatgc aaaacccgcc cctgcggcag gagatagtgg
     1861 attaacaaaa atcagga
//
LOCUS       DAXHKX010000238         1867 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXHKX010000238 DAXHKX010000000
VERSION     DAXHKX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.238"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            363..1808
                     /gene="zwf"
                     /locus_tag="ACFMWZ_002062"
     CDS             363..1808
                     /gene="zwf"
                     /locus_tag="ACFMWZ_002062"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC5935953.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          505 a          430 c          491 g          441 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatattttaa aaggtatgcc gtctgaaagt
       61 gggaagcggg tgttattata gtacaaaatt tgtaattttg ttacaaaaaa atcagacatc
      121 gaaaagaaat ttgatttaaa tcgcagaatc tttcgattgt tgtaacggaa taggggaaac
      181 ctgcccgatt tttggactag gtcaaataaa ttgtaaaaat gggaatagac aaaaagtgta
      241 gcggtgtgta gtattactac tcattatgcc gcctgccttt gtttccgata ttgcgatgca
      301 gcgtgatggg gaaaacaggt ttcaaacggc atcgaaaaat tctttcctga cgagataata
      361 cgatgagtac acaaacaaat tttgatttgg tgttgttcgg tgcgaccggc gatttggcga
      421 tgcgcaagct gttgccctgt ctgtaccagg cgcatgttgc cggtctgctt catcccgaag
      481 ggcgtatttt gggggtcagc cgcagcgagt tggataccga aggttttctg gcgaaggtgg
      541 aaaccagttc caaaatccat gtgaaagaaa atttttccga tgaggcttgg gcatcgtttg
      601 tcgaacgttt cgcatatctc aaagtcgatg ttacgcagcc ggacgatttt gccgctttgg
      661 gcgatttggt caaagcgcgc aaagaaaccg acaatgtcgt catctatctg tccaccgcgc
      721 cgaaattctt cgctcaaaca tgtgaaaacc ttgccaaaat cggtttgaac gccgacaacg
      781 tgcgtgtggt tttggaaaaa ccgttgggta cggatttggc ttcttcccag caaatcaata
      841 ccgatgttgc gcgttatttc aaagaagggc aaatttaccg catcgaccac taccttggca
      901 aagaaagcct gcaaaacctg cttgcgctgc gttttgccaa tgtaatgttc gagccgctgt
      961 ggaacaataa atacatcgaa agcgtgcagt tgaccatcgc cgaacagctc ggtgtggaag
     1021 agcgcggcga gttttacgac attaccggcg cgttgcgcga tatggtgcaa aaccatttga
     1081 tgcagatgct gtgcatgact gcgatggaag cccccgccag cttggatgcc gacgcggtgc
     1141 gcgatgaaaa agtcaaagtc atcaagtcat tgaagccgct gaccgtcgaa tctgtcaatg
     1201 aaaatgtcgt gcgcggacaa tataccgccg ccaaaggcat gaacggctat cttgaagaaa
     1261 tcaacgttcc gcaagacagc tttaccgaaa cctacgtcgc cattaaagcc gaaatcgaaa
     1321 acgaacgctg gaagggcgtt cccttctacc tgcgtaccgg caagcgcatg gcgggcaaag
     1381 tggcggaaat cgttttgaac ttcaaagatt tgaacagcca tatttttgaa ggcagtcgca
     1441 ccgcgcccaa ccggctcgtt atcgagttgc aaccatatga atccgtgcgc ctctatacgc
     1501 agatgaaaac cccgggtgca ggaaataagg tcgaaaccgt gccgctggca accgacttgg
     1561 gcaaagcatt ggaaggccgc cgcgccgagg cttacgagcg cctgctgttg gatgtgatta
     1621 acggcaaact cgctttgttc aaccgccgcg acgaactcga agccgcatgg gaatatgtga
     1681 tgccgatttt ggaaaactgg acaaataaca ccacgccgcc gcacggctac ggcgcacact
     1741 cgtgggggcc tgaagccgcg cgcgaactgt tggcgcgcga cggacacaag tggcacgaag
     1801 agcagtaata caataatgcg ttcagacggc atggggtttg aaatgccgtc tgaacgtaag
     1861 taaagca
//
LOCUS       DAXHKX010000239         1827 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXHKX010000239 DAXHKX010000000
VERSION     DAXHKX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.239"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            180..1145
                     /locus_tag="ACFMWZ_002063"
     CDS             180..1145
                     /locus_tag="ACFMWZ_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC5935954.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT          426 a          484 c          549 g          368 t
ORIGIN      
        1 atcaagcgca gggattggtt gaaacggaaa aggtcgtctg aaagggtgtt tcagacgacc
       61 ttttccgtat cgggaatttg ttttgccgta tcgggaattt tgcgttttgc ggtgtggttt
      121 ctgcaggttg tttgcttaat aataaacatt cttattcgta tgcaaaggaa ccgcacaccg
      181 tgaaaccgcg tttttattgg gcagcctgcg ccgtcctgct gaccgcctgt tcgcccgaac
      241 ctgccgccga aaaaactgta tccgccgcat ccgcatctgc cgccacgctg accgtgccga
      301 ccgcgcgggg cgatgccgtt gtgccgaaga atcccgaacg cgtcgccgtg tacgactggg
      361 cggcgttgga tacgctgacc gaattgggcg tgaatgtggg cgcaaccacc gcgccggtgc
      421 gcgtggatta tttgcagcct gcatttgaca aggcggcaac ggtggggacg ctgttcgagc
      481 ccgattacga agccctgcac cgctacaatc ctcagcttgt cattaccggc gggccgggcg
      541 cggaagcgta tgaacagttg gcgaaaaacg cgaccaccat agatctgacg gtggacaacg
      601 gcaatatccg caccagcggc gaaaagcaga tggagacctt ggcgcggatt ttcggcaagg
      661 aagcgcgcgc ggcggaattg aaggcgcaga ttgacgcgct gttcgcccaa acgcgcgaag
      721 ccgccaaagg caaaggacgc gggctggtgc tgtcggttac gggcaacaag gtgtccgcct
      781 tcggcacgca gtcgcggttg gcaagttgga tacacggcga catcggccta ccgcctgtag
      841 acgaatcttt acgcaacgag gggcacgggc agcctgtttc cttcgaatac atcaaagaga
      901 aaaaccccga ttggattttc atcatcgacc gtaccgccgc catcgggcag gaagggccgg
      961 cggctgtcga agtattggat aacgcgctgg tacgcggcac gaacgcttgg aagcgcaagc
     1021 aaatcatcgt catgcctgcc gcgaactaca ttgtcgcggg cggctcgcgg cagttgattc
     1081 aggcggcgga gcagttgaag gcggcgtttg aaaaggcaga acccgttgcg gcggggaaag
     1141 agtagggagt cgtctgaaaa cggagcttcc gaaggaagcg gggggtttct gcgaagctaa
     1201 agtgcggttt caacgaattg aaaagcagcc tgtatgttga aaataccgct caagcaaacc
     1261 tacggtttgc cgccctctcc ctagccctct cccacaggga gaggggattg gggtgcaggc
     1321 tgcctttaag gtttaggcaa atttttaact tcgttgaagc tgcgatttca gaagctccgt
     1381 tttagcttcg cagaaactcc gcttccttcg aaagctccgt tttcagacga ccttttggaa
     1441 caccacaggc acacgcatcg aacggctgaa tcaaagttca gaccgatggc agcccgcacc
     1501 cgagtttatg cggcaaacag cgaggctacg gcaacccgtc ccctctccct gtgggagagg
     1561 gttagggaga gggcagtaag ccgtaggctt acatcaaagc cgataacgct ttcacaaagc
     1621 ctgtgtgcac tgaaaactga aaggtcgtct gaaaacggag ctttcgaagg aagcagagtt
     1681 tctgcgaagc taaaaccgaa gctgcaaaaa atcgaaaagc agcctgcacg ttgaaaatgc
     1741 cgcccaagca aacctgcggt ttgccgccct ctccctagcc ctctcccaca gggagagggg
     1801 attggggtgc aggctgtctt taaggtt
//
LOCUS       DAXHKX010000240         1806 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXHKX010000240 DAXHKX010000000
VERSION     DAXHKX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.240"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(193..849)
                     /locus_tag="ACFMWZ_002064"
     CDS             complement(193..849)
                     /locus_tag="ACFMWZ_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC5935955.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFILFLLVFRLCWSIWGSDTARFSCFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRLFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            913..1803
                     /locus_tag="ACFMWZ_002065"
     CDS             913..1803
                     /locus_tag="ACFMWZ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC5935956.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLSKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          391 a          425 c          596 g          394 t
ORIGIN      
        1 aagtaaatcg gttctgtact atccgtactg tctgcggctt cgtctccttg tcctgatttt
       61 tgttaatcca ctataaaacg gttttggacg gaaaaacggc ctgaagccgt ttcgggcatt
      121 cagaccgttt gcgcggagag gggatgccgt ccgaagggcg aaaagggctt cagacggcat
      181 tgatgtcggg tttcaggaca ggagcaggat ggcggctgcg gcaagcgcgg caaccgataa
      241 tgcggcggca agcgcggctt tgccggcaaa gcggattgag gttttgcctt cgatgtattt
      301 gaagccggtt atcatcggga ggacgaggtt tttctttttg aataggcggt atgcggcgac
      361 ggcggcgata tggatggcgg aaaaaacggc gagcagcttg aaaaagttga ggtggatttt
      421 ccgcatcagg ctgcccgtat gctcggaaac caaatggttg aggtagccgt tggtgctgaa
      481 ggtgttttca tcggcggcaa aaagccctgt gccgacttga aatgagacgg cggccaaaag
      541 cgcgacgacc atcagtgcgc ccaaggggtt gtgtccgggc tggatgtgtt cgggaatacc
      601 gtttttcaga tagccgcgta tgcctgccca gccttggacg aaacaggaaa aacgggcggt
      661 atcgctgccc caaatgctcc agcagaggcg aaatacgagc aggaaaagga taaacagccc
      721 gacgcgcgtg tgccattgca gcatatcgcc gccggctttc gcgctatacc acataaaggg
      781 cagggacgcg gcaagcagcc agtggaaaag gcgggtgggg aggtcccaga ctttggtttt
      841 gtttttcata atcggtttcc ggcggtagaa tcggtttgtt ttcgagcctt attttaaccg
      901 attggagggg caatgtttcc cgtttttcat ctttcaggcg agagccgccg ccggatgctt
      961 cagacggcat tgcgttttcc ctatgttttc aaagcccgtg cggaagattc gcacaaaggg
     1021 actttcggca cgctcgccgt agtcggcgga tcggcaggga tgagcggtgc gcccgtattg
     1081 gcggcatcgg cggcgatgta tctcggctgc ggcaaggtgt gggcgggttt caatcaggat
     1141 gcgctacctt ttgccgttat tgccggtttt cccgagatta tgctggatac ggcagacggt
     1201 ttgagcaagc gtcaagatat aaacgcctgg gttgtcggtt gtggattggg tacaggtagg
     1261 gcggcggtcg gaacgcttgc cggaattttg acggaacaca cggacaagcc cgtccttttg
     1321 gatgcggacg cgctgaacat attatcaacc gatgccgaaa cccgaaatct ggcacgcggg
     1381 tgtaaaaacc tgattttaac gccacacccc gccgaagccg cgcgcctgct tggaacgacg
     1441 gttgcacagg ttcaggcgga tcggacggcg gcagtgagga agataggggc aattttcggc
     1501 gcaaccgtgg ttttaaaggg gcacaaaaca ttggttgccg cgtccgatac ggaaatctat
     1561 gtcaacgaaa gtggcaacgc gggattggca acggcgggca gtggcgacgt attgggcggc
     1621 atcatcggca gtctgctcgc acagggcgtg ccggtttttg aagccgcctg cgcgggcgcg
     1681 tggctgcacg gcgcggcggc ggatgtcata aaagaatcgg caggcattgc ggcagggctg
     1741 ttggctggcg aaatcgcccc ggcggcaagg tggctgcgta accggataac tgaaagtatg
     1801 taagaa
//
LOCUS       DAXHKX010000241         1776 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXHKX010000241 DAXHKX010000000
VERSION     DAXHKX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.241"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(544..1602)
                     /gene="alr"
                     /locus_tag="ACFMWZ_002066"
     CDS             complement(544..1602)
                     /gene="alr"
                     /locus_tag="ACFMWZ_002066"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC5935957.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT          444 a          446 c          405 g          481 t
ORIGIN      
        1 aaaatccctg tgtccttatt tctgctttct tacgtcattc ttttaaaggt gggaatctgg
       61 aaaccaaaaa acaacaggaa tttatccgaa acaacaaaac ctatccgccg gcatccccgc
      121 gaaagcggga atctagtttg ttgttcaaat aaattctgaa actttaattt tgtcattccc
      181 gcgcaggcgg gaatctagaa atgaaaaacg acgagaattt atcagaaatg acggaaactc
      241 aaaaaaccgg attcccgcct gcgcgggaat gacgaaattt taggtttctg attttgattt
      301 tccgtttttg cgggaaggat ggggtttcag attgtgggca ttatcagtat tttctatttg
      361 gcaatgtctt ttataatgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta
      421 cgatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatactg
      481 attttgtcaa aacgcattta tgccgtctga aaggcttcag acggcatttt tgtttggact
      541 tgattactcg atataagtga attttgcacg tttgatattg cacatcaatt cgtaagggat
      601 ggttccggcc gcttcggcaa cagtattgat gttgaccgta tcgccccaca gttcgacctc
      661 gtgtcccaaa ccttcttgcg aagcgtccag ctcgatggtc atcatatcca tagagaccct
      721 gccgatgacc cgggtcaatt tgccgtcgac agcgacgggg gaattgcttg gggcgcggcg
      781 cggataaccg tccgcataac cgcaggcaat caggccgacg cgcgtagatt tgctggtata
      841 aaatgttgcg ccataaccga taggggagtg cggctgtaaa acgcgttcgc cgaaaatacg
      901 ggttgaaagc ctcatcacgg gcttcagcct gtcatcgcct cctccgaacg gggaaatgcc
      961 gtataacgcc agaccggcgc gcccccagtc cctgcgtgct tcgggaacat tcaaaatagc
     1021 ggcggagttg gcaaggcttt cttcgccttc cagcccttcc gtacccaaat cgaatgcttc
     1081 catctgtatt tccgtcatac cgctttcggg ttcgtccgca caggagaaat gcgagaattt
     1141 gacaatactg tccacatatt ccgactgttt caatgccgca tatgccgaag cgtaatcatg
     1201 agggaaaaat ccggtacggt gcattcccga atccattttc aaccagacct tgacaggttt
     1261 tttccaatgg cgggaaagca aagcctcaag ttgccattgg tttccgactg ccggccagag
     1321 cgagtattgt tcgaccgctt cgtactctga tgcttcaaat acgccttcca aaaggacaat
     1381 cggatgggta atgccgctct cccgcagcct gattccttca tcgattgtcg ccacggcaaa
     1441 gccgtctgcc aagtctgcca gcgcgaaagc acatctgacc gcaccgtgtc cgtatgcgtc
     1501 ggccttcact accgccaaca gcttgcctcc gtgcatttcc tttaaaatcc gataattgtg
     1561 cctaaggttg cccaacctga tctgcacgtt caaagggcgc ataaattcat tccttaccaa
     1621 gcaagtgttt attagcaaaa ttaagaaatc aattagggaa ttataatcca acggcaaggc
     1681 ttttaaaata ttaaagaccc ttgtgtcttc aagccgaagc agcagttttc aggtaggaag
     1741 gcggtttgct tttcctgata taatcgcttt cccaaa
//
LOCUS       DAXHKX010000242         1776 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXHKX010000242 DAXHKX010000000
VERSION     DAXHKX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.242"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(26..349)
                     /locus_tag="ACFMWZ_002067"
     CDS             complement(26..349)
                     /locus_tag="ACFMWZ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC5935958.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="ACFMWZ_002068"
     CDS             complement(337..576)
                     /locus_tag="ACFMWZ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC5935959.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="ACFMWZ_002069"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="ACFMWZ_002069"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC5935960.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALENALAQI
                     AGEAVSTIVAGRTDTGVHATAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          400 a          415 c          506 g          455 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatttg ttcgggtaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatag gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagagact acgacacaag gacgtgtctt tttgatttcg cttcctacgg
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cccctgcgaa cggtaggaca gcttaaaatg atattgccct tttcaactgt
      481 aacagccaag ctgtctactg cccctatttg acccaataat gatttgggca gaatcacgcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatac gtaccatatt caccagttta
      601 tgtaataaca atgttattac cttgatgagg tagtgtcaac attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag cccgtcgggc
      781 ataaaggtcg gtggggcttt gaggcgactg cgttcttgaa tcagtgcggc gaagccttcg
      841 acgctgagtc tgccgctgcc gacatagacg agcgcgccca tgatgttgcg taccatgtgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgcgctttg ggtaaggtcg
      961 gcgcggtaga tggttttgac gggggatttt gcttggcatt cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccgatcaa ataggcggca gcccgacgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacgggggc ggattcgagc
     1141 aggtagcggt agtgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg tatccatgcc
     1261 tgttggggac gggcggcagt tgtgtcgaag tggacgactt gggcggtggc atgcacgccg
     1321 gtgtcggtcc tgccggcaac aatggtggaa accgcttccc ctgctatttg ggcaagtgcg
     1381 ttttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgggggg tggatgcagt tgctacgaat
     1621 cgctatcctg tgaattcacc ctgtcaggag tgcccgaatc gtcattctcg cgcaggcggg
     1681 aatctaggac gtaaaatcta aagaaaccat tttatagtgg attaacaaaa accagtatgg
     1741 cgttgcctcg ctttagctca aagagaacga ttctct
//
LOCUS       DAXHKX010000243         1770 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXHKX010000243 DAXHKX010000000
VERSION     DAXHKX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.243"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            294..1454
                     /locus_tag="ACFMWZ_002070"
     CDS             294..1454
                     /locus_tag="ACFMWZ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFC5935961.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGATRW
                     GNRESFVGLAGEFGTLRAGRVANQFDDASKAIDPWDSNNVVASQLGIFKRHDDMPVSV
                     RYDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQQTPQSQPTLVPAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKG
                     ENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          446 a          411 c          456 g          457 t
ORIGIN      
        1 gtgccgtgtg tgttttttat ggcgttttaa aaagccggga ctgcatccgg gcagcagcgc
       61 atcggcccgc acgaggtctg cgcttgaatt gtgttgtaga aacacaacgt tttttgaaaa
      121 ataagctatt gttttatatc aaaatataat catttttaaa ataaaggttg cggcatttat
      181 cagatatttg ttctgaaaaa tggttttttg cggggggggg gtataattga tgacatatcg
      241 ggtgtttgcc cgatgttttt aggtttttat caaatttaca aaaggaagcc gatatgcgaa
      301 aaaaacttac cgcgctcgta ttgtccgcac tgccgcttgc ggccgttgcc gatgttagcc
      361 tgtacggcga aatcaaagcc ggcgtggaag gcaggaacat ccagctgcag ttgaccgaac
      421 cgctccaaaa tattcaacaa cctcaggtta ctaagcgcaa aagccgcatc aggacgaaaa
      481 tcagcgattt cggctcgttt atcggcttta aggggagcga ggatttgggc gaagggctga
      541 aggctgtttg gcagcttgag caagacgtat ccgttgccgg cggcggcgcg acccgttggg
      601 gcaacaggga atcctttgtc ggcttggcag gtgaattcgg cacgctgcgc gccggccgcg
      661 ttgcgaatca gtttgacgat gccagcaaag ccattgatcc ttgggacagc aataatgttg
      721 tggcttcgca attgggtatt ttcaaacgcc acgacgatat gccggtttcc gtacgctacg
      781 attcccccga attttccggt ttcagcggca gcgtccaatt cgttccggct caaaacagca
      841 agtccgccta tacgccggct cattttgttc agcagactcc tcaaagtcag cctactctcg
      901 ttccggctgt tgtcggcaag ccggggtcgg atgtgtatta tgccggtctg aattacaaaa
      961 atggcggttt tgccgggaac tatgccttta aatacgcgaa acacgccaat gtgggccgtg
     1021 atgcttttga gttgttcttg ctcggcagcg ggagtgatga agccaaaggt accgatccct
     1081 tgaaaaacca tcaggtacac cgcctgacgg gcggctatga ggaaggcggc ttgaatctcg
     1141 ccttggcggc tcagttggat ttgtctgaaa atgccgacaa aaccaaaaac agtacgaccg
     1201 aaattgccgc gactgcttcc taccgcttcg gtaatgcagt tccacgcatc agctatgccc
     1261 atggtttcga ctttatcgaa cgcggtaaaa aaggcgaaaa taccagctac gatcaaatca
     1321 tcgccggcgt tgattatgat ttttccaaac gcacttccgc catcgtgtct ggcgcttggc
     1381 tgaaacgcaa taccggcatc ggcaactaca ctcaaattaa tgccgcctcc gtcggtttgc
     1441 gccacaaatt ctaaatagcg gggcggtgaa gcggatagct ttgtttttga cggcttgcct
     1501 tcattctttg attgcaatct gactgccaat ctgcttcagc cccaaacaaa aatccggata
     1561 cggaagaaaa acggcaataa agacagcaaa taccgtctga aagattttca gacggtattt
     1621 cgcatttttg gcttggtttg cacatatagt gggggactgt tagtttttgc acaaggaaca
     1681 aatagagtaa aaaaacgctg aaatctcgga aagacgtgga tttcggcgtt tttttgcatc
     1741 cggaaaagtt acgccagctt tttcacgaaa
//
LOCUS       DAXHKX010000244         1733 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXHKX010000244 DAXHKX010000000
VERSION     DAXHKX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.244"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            356..1660
                     /locus_tag="ACFMWZ_002071"
     CDS             356..1660
                     /locus_tag="ACFMWZ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC5935962.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          354 a          434 c          511 g          434 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtctgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taa
//
LOCUS       DAXHKX010000245         1709 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXHKX010000245 DAXHKX010000000
VERSION     DAXHKX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.245"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..858
                     /gene="mafA"
                     /locus_tag="ACFMWZ_002072"
     CDS             <1..858
                     /gene="mafA"
                     /locus_tag="ACFMWZ_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC5935963.1"
                     /translation="RFAVEQELVAASSRAAVKEMDLSALKGRKAALYVSVMGDQGSGN
                     ISGGRYSIDALIRGGYHNNPESATQYSYPAYDTTATTKADALSSVTTSTSLLNAPAAA
                     LTKNSGRKGERSAGLSVNGTGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVVPPEY
                     ADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYES
                     QYQEQYALWMGPYSVGKTVKASDRLMVDFSDITPYGDTTAQNRPDFKQNNGKKPDVGN
                     EVIRRRKGG"
     gene            862..>1709
                     /gene="mafB"
                     /locus_tag="ACFMWZ_002073"
     CDS             862..>1709
                     /gene="mafB"
                     /locus_tag="ACFMWZ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC5935964.1"
                     /translation="MKPLRRLIKLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADGYDGPQGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AVIGGLG"
BASE COUNT          444 a          622 c          392 g          251 t
ORIGIN      
        1 cgctttgccg tcgaacaaga actcgtcgcc gcatcgtccc gcgccgccgt caaagaaatg
       61 gatttgtccg ccctgaaagg acgcaaagcc gccctttacg tctccgttat gggcgaccaa
      121 ggttcgggca acataagcgg cggacgctac tctatcgacg cactgatacg cggcggctac
      181 cacaacaacc ccgaaagtgc cacccaatac agctaccccg cctacgacac taccgccacc
      241 accaaagccg acgcgctctc cagcgtaacc acttccacat cgcttttgaa cgcccccgcc
      301 gccgccctga cgaaaaacag cggacgcaaa ggcgaacgct ccgccggact gtccgtcaac
      361 ggcacgggcg actaccgcaa cgaaaccctg ctcgccaacc cccgcgacgt ttccttcctg
      421 accaacctca tccaaaccgt cttctacctg cgcggcatcg aagtcgtacc gcccgaatac
      481 gccgacaccg acgtattcgt aaccgtcgac gtattcggca ccgtccgcag ccgtaccgaa
      541 ctgcacctct acaacgccga aacccttaaa gcccaaacca agctcgaata tttcgccgtt
      601 gaccgcgaca gccggaaact gctgattgcc cctaaaaccg ccgcctacga atcccaatac
      661 caagaacaat acgccctctg gatgggacct tacagcgtcg gcaaaaccgt caaagcctca
      721 gaccgcctga tggtcgattt ctccgacatc accccctacg gcgacacaac cgcccaaaac
      781 cgtcccgact tcaaacaaaa caacggtaaa aaacccgatg tcggcaacga agtcatccgc
      841 cgccgcaaag gaggataaac cgtgaaaccg ctgcgaagac tcatcaagct ccttgccgcc
      901 tgtgccgtag cggcggccgc actcatacag cccgccctcg cggcggactt ggcgcaagac
      961 ccgttcatta ccgataacgc ccaacggcag cactacgaac ccggcggcaa atatcacctc
     1021 ttcggcgacc cgcgcggcag cgtttccgac cgcaccggca aaatcaacgt catccaagac
     1081 tatacccacc agatgggcaa cctgctcatc caacaggcgg caatccaagg caatcttggt
     1141 tacaccgtcc gcttttccgg acacggacac gaagaacacg cccccttcga caaccacgcc
     1201 gccgacagcg caagcaaaga acaaggcaac gttgacgaag gctttaccgt ataccgcctc
     1261 aactgggaag ggcacgaaca tcatcccgcc gacggctatg acggcccgca gggcggcaat
     1321 taccccaaac ctacgggagc acgcgacgaa tacacctatc acgtcaacgg cacagcacgc
     1381 agcatcaaac tcaatccgac cgacacccgc agcatccggc aacgcatatc cgacaattac
     1441 agcaacctcg gcagcaattt ctccgaccgc gccgatgaag ccaacagaaa aatgttcgag
     1501 cacaatgcca agctcgaccg ctggggcaac agcatggagt ttatcaacgg cgtcgccgcc
     1561 ggcgcgctca acccctttat cagcgcgggc gaagccttgg gcataggcga catactgtac
     1621 ggaacgcgct atgccataga caaagccgca atgcgcaaca tcgccccctt acccgccgag
     1681 ggcaaattcg ccgtcatcgg cggcttggg
//
LOCUS       DAXHKX010000246         1672 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXHKX010000246 DAXHKX010000000
VERSION     DAXHKX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.246"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(20..682)
                     /locus_tag="ACFMWZ_002074"
     CDS             complement(20..682)
                     /locus_tag="ACFMWZ_002074"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC5935965.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(721..1548)
                     /locus_tag="ACFMWZ_002075"
     CDS             complement(721..1548)
                     /locus_tag="ACFMWZ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC5935966.1"
                     /translation="MKPDIYALLERALLSGNPDEKGQLADEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYSDEVGHVAIGNRWYQHLCRE
                     RGLEPIALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
BASE COUNT          322 a          443 c          510 g          397 t
ORIGIN      
        1 tttgtggggc ggatattttt cagggctgta ttttgtccag acattcgagc agatcgagtg
       61 gcgtgcggat gtggaaatcc gcctgccatg agccggtatc gtcttcggga gcgatgtagc
      121 cccattcggc gaggacggtt ttcatgccgg cgttgcggcc tgcttggatg tcgcgttcgg
      181 catcaccgac gtagagtgtg tgttgcgggt cggcgtggat ttgtccgcac gcatacagca
      241 tgggtttgat gctgggcttg ggttcgccgc aggtgtcgcc gctgacgacg acggcgggtg
      301 ggatgatgaa gccgagtttg gggacgagtt tgtcggtgaa gcgcatgggt ttgttggtga
      361 tgatgcccca tttgatgccg cgttggttga gttcggcaat gagttcgttc acgccgtcaa
      421 agagctcggt gtcttgggcg tagcggcggt cgtattcctc cagatattcg gtgcgccatc
      481 gggcatagtc ggggtggtcg ggggtgatgc ctgcgccgag tttgatcagt cctgccgcgc
      541 cgtggctggc ttgggtgcgg atctcgtcca tgctttttgc aggtagtccg tggcgagcga
      601 gcagggtgtt gagtgcgcct ccgaggtcga gggcggtgtc ggcgagcgtg ccatcgaggt
      661 cgaacaatac ggcttgtatc atgtgttttc cttttttata aagtgcggga ctgaagggtt
      721 tcagacggca tgtttatttt gtttcaaacc ctgctcgaaa tcttccagca tatccagctc
      781 gaagcggctg aagcccgctt tttcccgcgc ttcgatgttc acatagccgc ggaagataaa
      841 catatcgtaa cgggcaatca gactgcggaa cagggcaatc ggttccaaac cgcgttcgcg
      901 gcaaaggtgt tgataccacc ggttgccgat ggcgacgtgt cccacttcgt cgctgtaaat
      961 gatgtccaac acgccgcagg tttccgaatc gccgcgctgc gccaccttcg cgcgtatccc
     1021 cggtgtaacg tccagcccgc gcgcttccaa cacgcgcggt accaacgcca tacgcaacag
     1081 cgggtcgtag gcggttttgt acgccatatc ccacaaatgg ttgtgcgcct cgaaactgcc
     1141 gtaatcgaag ccgaaagcgc gcaatctgtc gcgcatcagg cggaaatggt acacctcttc
     1201 cttcgccact ttcacccagt cgcggacaaa ctgaaacggc agcgtgcgga aacggtatgc
     1261 cgcgtccaaa gccagattga cggcgttgaa ttcgatatgc gcaatcgcat gcagcatcgc
     1321 cgcatagcct tcggttgtgt tcattttgcg cggggtcagt tgcgacggcg cgaccaaaac
     1381 aggcttgtcc ggccgtcccg ctcgggggaa gtccgccggc ggcgcgtttg tttccgcccc
     1441 gtccgcattt tgaacggcgg caaacgcctc atccgccagc tgtccttttt catcggggtt
     1501 gcccgaaagc agggcgcgtt ccagcaaagc ataaatatcg ggtttcattt caagtccgcc
     1561 gtgttcggaa aacaaatatt atagcgttta aaaaaaacaa gatgaggcat ataatctccg
     1621 cgattcggca ttccgcgccc aaaccgtcaa atatagtgga ttaacaaaaa cc
//
LOCUS       DAXHKX010000247         1672 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXHKX010000247 DAXHKX010000000
VERSION     DAXHKX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.247"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(4..1077)
                     /locus_tag="ACFMWZ_002076"
     CDS             complement(4..1077)
                     /locus_tag="ACFMWZ_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC5935967.1"
                     /translation="MRPFCVGATVLAILGALVFFISPGVVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPATTLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAW
                     LIWLARNTDNFALLMLLAAFTVFQTAYAATGDLNLLRAQVHLNMAAVMFVSIRVSVLL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHTAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGDMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCLIAVLLLFMAAVSRAFLMNVNPIFFITVPAILTAV
                     VFTLYLFTFVPIFRANAFTGDPE"
     gene            complement(1125..>1672)
                     /locus_tag="ACFMWZ_002077"
     CDS             complement(1125..>1672)
                     /locus_tag="ACFMWZ_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC5935968.1"
                     /translation="ADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDESDKQIDDHT
                     RTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAPHPRESGETKF
                     KSRCFGGRSAVRKALYMATVAATRFEPLIRDFYQRLPSEGKPYKVAVTACMRKLLTIS
                     NARMRDYFAENDTAENGIRTA"
BASE COUNT          428 a          443 c          472 g          329 t
ORIGIN      
        1 tttttattcc gggtcgcccg taaacgcatt cgcccgaaaa atcggaacaa acgtgaaaag
       61 ataaagcgtg aacacgacgg cggtcagaat cgcggggacg gtgatgaaga atattggatt
      121 cacgttcatc aagaaagcac gcgagacggc agccataaag agcaggagga cggcgatgag
      181 gcagagtttc gggtagtcga gcttggtaaa gccgctgtgc cacagtccgg cggtcagcca
      241 caccatcatc acgccgccca tcatgtcgcc gagggtaatc aggtgcaggg gcgcggaggc
      301 gggcaggttt tgcagtttcg ccgcacctgt ccacaaatag cctgcggcgg caaaaagctg
      361 gagcaggtaa taagtgcgga cgtagtgttt acgcaggagt tcgtgatggt gcagttcgcg
      421 cagtttggca agcaggataa agccgacggc gagcgcggta aaaccggcgg tctgcgcggg
      481 cagccaaagt tcggcggcgg tgtgcagcag caggaaggtg atggcgatgt ttttatagac
      541 gatgttgggg atgaatacgg ggtctttcag acggcattct ttaagggctt ccgcgcccag
      601 caaaacactc acacgtatgg aaacaaacat caccgcagcc atatttaaat gcacttgcgc
      661 gcgcaacagg ttcaagtcgc cggttgccgc gtatgccgtc tgaaaaacgg tgaacgcggc
      721 aagcagcatc agcagggcga agttatccgt gttgcgggcc agccaaatca accaagcgca
      781 gaacagcaat aaaaccagcc aataagccgc tacaaagaat gcggcaagtt gcggtaaaaa
      841 cggcaataaa acagccgcaa caagcaacaa caccgccatc aaagtagtgg caggtttcag
      901 gttgcccgaa aaacccgtcc agtccaacaa agccgcagtc aggaaaccac catatgctgc
      961 aggcagcatg agttctaaga agatttggcg gtgcaggacg acgacaccgg ggctgatgaa
     1021 aaacaccaac gcgccgagta tggcaagcac tgtcgcgccg acgcaaaacg ggcgcatagc
     1081 aacagtattt ttcaccccgt cgggcaaaaa taccaaaact caaatcaagc cgtccggata
     1141 ccgttttcgg cggtatcgtt ttcggcaaaa taatcacgca tccgggcatt cgatatcgtc
     1201 agcagtttac gcatacatgc cgtaacggca accttatacg gcttaccctc ggacggcagg
     1261 cgttggtaga aatcccgaat aagcggttca aaacgtgtcg ctgccacggt agccatatac
     1321 agtgccttac gcaccgcaga ccttccgccg aagcagcggc ttttgaattt ggtttccccg
     1381 ctctctctcg ggtgcggggc aatgccggcc aaactcgcta tccgtttgtg cgacagccgc
     1441 cccaattcgg gcagcatcgc catcagcgta gccgtcgtta tcgaaccgat gcctttgatt
     1501 tgctctgcca cttgggcttt gccgtcaaaa tgcgtgcggg tgtggtcgtc gatttgtttg
     1561 tccgattcgt caatcagccg gtcaaaatgg gcaatcagtt gtttgacgct tccgacttgc
     1621 gtttcgtgaa cctgatgcag acggtttttc tcggcagtcc gcatatccgc cg
//
LOCUS       DAXHKX010000248         1667 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXHKX010000248 DAXHKX010000000
VERSION     DAXHKX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.248"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(123..551)
                     /locus_tag="ACFMWZ_002078"
     CDS             complement(123..551)
                     /locus_tag="ACFMWZ_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC5935969.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(532..966)
                     /locus_tag="ACFMWZ_002079"
     CDS             complement(532..966)
                     /locus_tag="ACFMWZ_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC5935970.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKASGDDGVRKS"
     gene            complement(1129..1667)
                     /locus_tag="ACFMWZ_002080"
                     /pseudo
     CDS             complement(1129..1667)
                     /locus_tag="ACFMWZ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          406 a          483 c          339 g          439 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcatccc
       61 ctgcaaacca aaaaaaagac cgtctgaata cagacggcca aagcctggtc acacatcact
      121 gcctaaaata aagctgcctg ctgtgccgca ggtcggctca tttcattgat aatcgtatat
      181 cccgttcgtg aagagatgcc gtatttaggg catagcttcg tcatcgccat aagcccgctc
      241 tccttatcaa tatcgcgcaa tttgacaaac tcctgataaa acctatggtt tctcaactgt
      301 attaacgcct tgccgcaccg tgggacatac aattcctcgc caccatatac ctgcaacagc
      361 tcatgtgttt tcacttcgcc gatggcttcg accaaaattg ccaaacgctc ggtgtccacc
      421 ttgcccttac caaatttaaa ccgcgccccg ccaatcgcct tgaccagctg ttccgtcgct
      481 gccagtccga tgacatccac aatgtccaac acggtatccg gcaataaatg ttcaactttt
      541 tcgaacccca tcatccccac tcgctttttc cgttttcctg ttttccgcaa tctgcaacgc
      601 agcaaccagt ttatgtagct gcgtatcgtc taaatattcg accttatcct taccaaacat
      661 ccgccgcgcc attgcgtgtg catagttcca atgtttgccg ccgacggtca gcagggcttc
      721 gactttgtcc agcattgccg ctgatgatgt ccgacgcaga tgcggtttgc cgtgtgggtt
      781 accttttgct ttaggcttaa atccgtgcga ccgcatatca gcgacaacag actcaagttc
      841 agaaacatcc atatccgcac acgaccgctt atccgtcaca cgctccaaca ccgcgcgata
      901 ggtaccgtca tccaagccca gctccttttg agcaatctta attttcgcaa tcaacgcccg
      961 gcgcattatc tctctaatac aacatatagt ataaattagc gcattttata caataaaata
     1021 caatatatag tattaagccg atgttttttt gcgaaacgga cagacataaa aaagcaactg
     1081 tatttttcac cccgtcgggc aaaaatacca aaactcaaat caagccgttt agataccgtt
     1141 ttcggcggta tcgttttcgg caaaataatc acgcatccgg gcattcgata tcgtcagcag
     1201 tttgcgcata catgccgtaa cggcaacctt atacggctta ccctcggacg gcaggcgttg
     1261 gtagaaatcc cgaataagcg gttcaaaacg tgtcgctgcc acggtagcca tatacagtgc
     1321 cttacgtacc gcagaccttc cgccgaagca gcggcttttg aatttggttt ccccgctctc
     1381 cctcgggtgc ggggcaatgc cggccaaacc cgctatccgt ttgtgcgaca gccgccccaa
     1441 ttcgggtagc atcgccatca gagtagccgt cgttatcgaa ccgatgcctt tgatgtgctc
     1501 cgccacttgg gctttgccgt caaaatgcgt gtgggtgtgg ttgtcgattt gtttgtccaa
     1561 ttcgtcaatc agccggtcaa aatgggcaat cagttgtttg acgctttcga cttgcgtttc
     1621 atgaacctaa tgcagacggt ttttctcggc agtccgcata tccgccg
//
LOCUS       DAXHKX010000249         1650 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXHKX010000249 DAXHKX010000000
VERSION     DAXHKX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.249"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            84..1253
                     /gene="metZ"
                     /locus_tag="ACFMWZ_002081"
     CDS             84..1253
                     /gene="metZ"
                     /locus_tag="ACFMWZ_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC5935971.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLVDDLKQALAR"
     gene            1340..1546
                     /locus_tag="ACFMWZ_002082"
     CDS             1340..1546
                     /locus_tag="ACFMWZ_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC5935972.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          401 a          435 c          437 g          377 t
ORIGIN      
        1 gtaccggttt aaatttaatc cactataaat taaacaacgg gcgcacgctc cgttgccgaa
       61 cggataaaac cgaggaatca ccgatgagca aaaaactcca tccgcaaacg ctcgccatac
      121 gcggcggcaa agaacaaacc ggataccgcg aacacaatca ggcattgttc ctgaccagca
      181 gtttcatgtg ggacaacgcc caacacgctg ccgatttgtt ttcaaaaaaa atcaaaggct
      241 tcacttatac ccgtaccgcc aacccgacca ccgccgcctt tgaaaaacgc atcgccgctt
      301 tggaaggtgc ggaacgcgcg gtcgccactt cgaccggcat gtccgcgatt caggcggcgt
      361 ttttcacctt tttgcaggcg ggcgaccatg tgatttccag ccgcagcctg ttcggcacga
      421 ccgtcggctt tatcaataac atcgttacca aattcggcat cgaagtgagc cacgtgtcgc
      481 cgactgatat aaacgaatgg aaagccgccg tcaaagccaa caccaaactg ctgtttttgg
      541 aaacgccgtc caatcccttg ggagaagtgg ccgacttgga agccttggcg gaattggcgc
      601 acggcatcgg cgcgattttg gtggtggaca acagcctgct gtcgcccatc ggctctcagc
      661 ctttgaagca cggcgcagat atttccgttt cttccgcaac caaagccata gacggacacg
      721 gacgcgtgat gggcggcgtg ttggcgggtt cggaagaact gatggcgcag gccgccgttt
      781 actgcaactc ttgcgggctg gcgatgtcgc cgtttaacgc gtggcagttg ttgagcggcg
      841 tggaaaccct gtcgctgcgt atggaaaaac agttcgacaa cgccctgaaa atcgcacaat
      901 ggctgcaagc gcagccgcag gttcaagccg tgtactacac tggattgccc gaccatccgc
      961 aggcggagct tatccgcaaa cagcaaaacg gcggcggcat cgtcatcggc tttgaagtcg
     1021 ccgaccaagc agccgcgtgg aaagtcgtgg acggcgtgga actcttttcc cgtaccgcca
     1081 acctcggcga cgtgcgctcg accatcaccc atccgtggac aaccacgcac ggcaggatgc
     1141 agcccgaaga aaaactcgcc gccaacatcc gccccggatt ggtgcgcctg tcggtcggtt
     1201 tggaatatgt tggcgacctc gtcgatgatt tgaaacaggc gctcgcgcgc tgattgcgtg
     1261 tttcagacgg cattggacag cagatgccgt ctaaaaccct tatcttatat gacatacacg
     1321 tcaacaatag gaaaaaaata tggatgaagc ggtttttgcc gattgggcgt tgaaaatctg
     1381 cctgaccggc ttgattattt ttttgggttt tatcgtttgg aatttgggca aggagtccaa
     1441 agcaggcaaa ttcggcatcg ccattttgtt tttggttttg ggcttgggcg tgttcggttt
     1501 tgtgttcaaa gagttattga ttaagttttt ggtattgccg aaataggttt gcggtctgtc
     1561 aaaatgccgt ctgaaatcat aaaaagtaat ctgcacccta aaagtttgat ttaaccgcca
     1621 actgattaag gtgcggattt ttatgacaaa
//
LOCUS       DAXHKX010000250         1591 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXHKX010000250 DAXHKX010000000
VERSION     DAXHKX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.250"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..785)
                     /locus_tag="ACFMWZ_002083"
     CDS             complement(<1..785)
                     /locus_tag="ACFMWZ_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC5935973.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAGWLVVGA
                     ILGGGAGAWWGSLGAEKAWRWFEDFLNLNRSDKYHVYDPLALD"
     gene            complement(1381..1591)
                     /locus_tag="ACFMWZ_002084"
                     /pseudo
     CDS             complement(1381..1591)
                     /locus_tag="ACFMWZ_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          522 a          346 c          198 g          525 t
ORIGIN      
        1 aaatctaggg cgagggggtc gtaaacgtgg tatttgtcgc tgcggttaag atttaagaaa
       61 tcctcaaacc atctccaagc tttttcagct cctaatgatc cccaccaagc tcctgcgcca
      121 cctccaagta tagctccaac tactagccaa cctgcgccac cagtaagtaa accaaaagcc
      181 ccaacaccca aagccgcacc ccattggacg cccattagcc cacccaaaaa agaagctgat
      241 gagagaccaa catcactcca atttccttct ccatccaagg ctttccacca agcacttccc
      301 atactcaaag ttcccatggc aacagttgcc ccataagtaa acttacccaa attcgctgcc
      361 ataacttttg attttgaagc attctctaac gctgttgccg cacgtcttgc atattgctgc
      421 ttgttatagt tgctagcatt caatgctttt tgcaaatcac tttgtacttt tcttgcaaca
      481 aatgcctcct ttgaattacc acgaaattta ctagcagctc ttccaattcc ttcttttaag
      541 cttttttgtg cctcttctgc tgtgcgttgg aattctcttg ccttagcaga cagacgctca
      601 ccctccctta aaaattcttt ttgatctatc ttgtgcgaac gggtcaaata ttcttcgtac
      661 attgagttaa tcgtaccaat cgccctaacc acattttcat cagaaaatat ggaaataata
      721 gcatccctat acgcacctag tgtaatattg tttctattat tagttatagc attattcgaa
      781 tacataatag cacctccaaa tttagaactg aatacgttta tattctctgt gtaataatat
      841 caaataatta attaatggat aagtaagtcc gaaaaaataa caactaaaat aagcaatata
      901 ctgattctct cctttattaa ttagatatat tgaaaaacaa ataaaaaata aacagaaaac
      961 attcatgtga aaaagccacc tcatattctt atttataagt ttaaaataac taggcattgt
     1021 atatttaaaa atcggcacat ccaacggaaa caaaccacat aaaaattttt ctttattctt
     1081 tattaaagac atagttttat tttttaaaat tagtccaaat aatatactaa caattactat
     1141 taaaacaata agcacagcgt aattttttat aaaattcatt aaaataataa cgaaaatact
     1201 aaatagatta catactataa attgaaatat aaaatattca gtatttaatc cattttcaac
     1261 cgcgagtttt ttaaaatatc cttgctcgaa aaaattaaaa aaatccatta atactttcct
     1321 ctcaagaaaa ctaattttag tttagttaag ataaaatgca atattacctt tattttctgc
     1381 ttttgaagca ttttccaaag ctgtgactgc ttgtcttgca tattgttgct tgttatagtt
     1441 actagcattt aacttctttt gcaaatcact ttgcattttt cttgcaacaa atgcctcctt
     1501 cgaattggca tgaaatttac tagcagccct tccaatccct tcttttaaag ccctttgagt
     1561 tttttctgct gagtgctgaa aatctttcgc t
//
LOCUS       DAXHKX010000251         1573 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXHKX010000251 DAXHKX010000000
VERSION     DAXHKX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.251"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..1364
                     /locus_tag="ACFMWZ_002085"
     CDS             <1..1364
                     /locus_tag="ACFMWZ_002085"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC5935974.1"
                     /translation="KIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDIYGSLALTG
                     YGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIPDRDLNIRG
                     NQVLPKHPNGLRFTAYASDGIVLKEQVYYSTGGGFVVTGEDFERQSETEKTVPYPYNS
                     CAELLDRCRLNRLDISEVVLANEAALAGCSANEIRRRVAGVADVMEGCIKRGLAADGE
                     LPGGLNVRRRAPHLATKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRVVTAPTNGA
                     AGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGCQGEVGVAC
                     SMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNGIAAEKALK
                     LGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKSTYKETSLAGLAATLRKKAVPVSVRV
                     VEC"
BASE COUNT          353 a          502 c          429 g          289 t
ORIGIN      
        1 tcaaaatcgg tatcgggcct tccagttcgc atacggtcgg cccgatgaag gcagccgccg
       61 cctttgcggc aggtttggat gcacaggctg ttcgcatcgt catcgacatt tacggctcgc
      121 tcgccctgac cggatacgga cacggtacat ttgacgcgct gatgctcggt ttggaaggca
      181 gcctgccgca cgacatcccg cttgccggca ttcccgaacg cctcgaacgc atccgcacgc
      241 agcacatcct ccggctcaac gggcaagaaa tccgcttcat ccccgaccgc gacctgaaca
      301 tacgcggcaa tcaagtgctg cccaaacacc ccaacggcct gcgatttacc gcctatgctt
      361 cagacggcat cgtcttgaaa gaacaggttt attactcgac cggcggcggg tttgtcgtta
      421 ccggagaaga tttcgagcgg cagtcggaaa cggaaaaaac tgttccttat ccttacaaca
      481 gttgtgccga gctgcttgac cgatgccgtc tgaaccggct cgacatctcc gaagtcgtgt
      541 tggcaaacga ggccgcgctt gccggatgca gtgcaaacga aatccgccgc cgcgttgccg
      601 gcgttgccga tgtgatggaa ggctgcatca aacgcggttt ggcggcagac ggcgagctgc
      661 ccggcggatt gaacgtccgc cgccgcgccc cgcatcttgc caccaagctc aaagtcctgc
      721 gcgaaaccga aatcgtcaac acccagctct ggccgatggt gtacgccatg gcggtcaacg
      781 aagaaaacgc cgccggcgga cgcgtcgtta ccgcaccgac caacggcgcg gcaggcatca
      841 ttcccgccgt attgcactat ttccgcaagt tcaatccgca cgccacacag gaacgcgtcg
      901 aaaacttcct gctcaccgca ggcgcaatcg gcatcctcta caagaccaac gcctccattt
      961 ccggtgcgga tgtcggctgt cagggcgaag tcggcgtagc gtgttcgatg gcggcgggcg
     1021 catacgccga agtcatcggc ggcacgccca aacaagtgga aaacgccgcc gaaatggcga
     1081 tggaacacca tttggggctg acttgcgacc ccgtcggcgg actggtgcaa atcccctgca
     1141 tcgagcgcaa cggcatcgcc gccgaaaaag ccctcaaact cggcacgctc gcgcttttgg
     1201 aagacggcac ggacaaaaaa gtctcgctcg acgaagtcat ccgaaccatg ttacagacag
     1261 ggcgcgacat gaagtccacc tacaaagaaa cctcgcttgc cggactcgcc gccacgctcc
     1321 ggaaaaaagc cgtccccgta tccgtgcgcg tggtcgagtg ctgaggatgg acagaaacga
     1381 aaatgccgtc tgaaaacagt tttttagacg gcatttcatt tatttcttgc ggctttaccg
     1441 tttcagtgcc agcgtcaaca cgcccgccgt aaccagcccc aagcctatcc attcctgcgt
     1501 gttcgggcgt tcgtctaaga aaaccaccgc catcaggaat gcaaaccggc gttgtttgca
     1561 gcatccggca aga
//
LOCUS       DAXHKX010000252         1566 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXHKX010000252 DAXHKX010000000
VERSION     DAXHKX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.252"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(389..1312)
                     /gene="lpxC"
                     /locus_tag="ACFMWZ_002086"
     CDS             complement(389..1312)
                     /gene="lpxC"
                     /locus_tag="ACFMWZ_002086"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC5935975.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          403 a          369 c          405 g          389 t
ORIGIN      
        1 gtgggaatga cgggatttga gattgcggca tttaccggaa aaaacagaaa ccgctccgcc
       61 gtcattcccg cgcaggcggg aatccagacc ttagaacaac agtaatattc aaagattatc
      121 tgaaagtccg ggattctaga ttcccacttt cgtgggaatg acggcatcag tctgccgttt
      181 acagcacggt ttctttagat tttacgttct agattcccgc ctgcgcggga atgactgaaa
      241 ctcaaaaaac tggattccca ctttcgtggg aatgacggga tgtaggttcg cgggaatgac
      301 gaattttagg tttctgtttt ggttttttgt ccttgtagga atgatgaaat tttaagtttt
      361 aggaatttac cggaaaaaat agaaagcgtt atccacaagt tctgatgttc agctcgtgaa
      421 atgcgtcggg caaatcatcg ctgtcggcaa attccacccg gtcgtaagcc gtttcgtctg
      481 ccaaaaccgc gcgcaagagt gcgttgttga tggcgtgtcc cgatttgtag ccttcaaacg
      541 cgccgacaat cggatgtccg acgatataca aatcaccgat ggcatcaagg attttgtggc
      601 gcacaaactc atcgggatag cgcaagcctt caggattcag gacatccgtg tcgtcaatca
      661 cgatggcgtt gttcaaattg ccgcccaaac ccagattgtg ggcgcgcatc atttccactt
      721 cgtgcataaa gccgaaagtg cgcgcgcgcg cgatttcgtc gatgtaggat ttgccggcga
      781 aatcgatttc aaaagtgggc gagctgcggt tgaaaaccgg atggtcgaat tcgatggtca
      841 gcgttacctt aaagccgtca tacggcgtaa agcgcaccca tttgcccgct tctttgattt
      901 cgacaggctt gaggattttc aaaaaacgct tttgcgcctt ttgatcgacc acgcccgcat
      961 cttgcaaaag gtaaataaac ggcaggctgg agccgtccat aatcgggatt tcgggtgcgt
     1021 tcagctcaat cagcgcgttg tcgataccgt aggcggacag cgcggacata atgtgttcga
     1081 tcgtgccgac gcgcacgcct ttgtcggtaa cgatggtgga ggaaaggcgg gtatcgttga
     1141 tcaaataagg ggtcagcttg atttgttcgc ccatctcgcc gtccaaatcg gtacggcgga
     1201 aggaaatccc gctgttttca ggcgcggggt gcagggtcag tgcgacccgt tcgccggaat
     1261 gcaggccgac tccggtaacg ctgatggatt tcgccaaagt tctttgcagc ataaaccgct
     1321 tccttatcaa tggggtaagt tggggaatga tacgataaaa ccggaaaaac aagctatgtt
     1381 tttccatagt atttgccaat gtatccgttt tcaatacgta agctgcataa aaatgtatag
     1441 tggattaaca aaaatcaaga caaggcgacg aacccgcctc cctcctgaaa aacgcaaaaa
     1501 atgccgtccg aaaacctttc ggacggcatt ttcgcgtgaa ccgtcattcc cacaaggaca
     1561 taaaac
//
LOCUS       DAXHKX010000253         1512 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXHKX010000253 DAXHKX010000000
VERSION     DAXHKX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.253"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(46..504)
                     /locus_tag="ACFMWZ_002087"
     CDS             complement(46..504)
                     /locus_tag="ACFMWZ_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935976.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(1068..1271)
                     /locus_tag="ACFMWZ_002088"
     CDS             complement(1068..1271)
                     /locus_tag="ACFMWZ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935977.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT          524 a          274 c          187 g          527 t
ORIGIN      
        1 gggaaagatt tacgatcgat tccgttgtat tttcgcaaga cgcgtttagt ctagagtctg
       61 tatattacat ttatttttag ggtctgctag ccaatttctt gttcccttca ttattttatc
      121 ttctgaaaga aaattatttt tttccatgct attaatatta atgatatgat ttttatttaa
      181 aataattttt ccatataaaa tttccggttt gtaatagcaa gagtctgaag caaatccttg
      241 cccgtcaata taagaaaaat aaatgttatc atcctcctta taatattcta aaaccaatcc
      301 ggatattatt ttatcaaaat tcccttcatt tccatctttc atgactaaaa tataattagc
      361 aggccaacct ttatcgtaaa catattcaaa ctgatagttc ccgaaactct cgatatccga
      421 actaaaaaag aagaaaagca aattaatagc caatacaaaa acaaataaga ataaaaatag
      481 caaaattttc aacttagtta acaatattta cctctccttt aaattcaatc ctgaaaggta
      541 ccccttaccc tgggctacca attatagttt ccatatttct aaatattgtt tttacattac
      601 ttttttctcc ccccaaagga attcatttta aaatcatgct tttcaggtgc taatcgatac
      661 ttaccattaa catctttaat cacagatata tttccatgta tagcccaacg tgaaaaatct
      721 gagtattata tacagttata ccttactgaa acaaagctag ctgtacatat ctgtaccata
      781 tttttactac aaacaaaaaa tatttataat tattaaggat aagataaaaa cccccaacgc
      841 cagattaatt cttaataatt tcaaataaaa aatatttgag attttttctt ttccaaataa
      901 tatattgaaa tatttaaagt taaggtagct tgatttaagt agaaaatcaa aaaaaccatt
      961 aattaatttt tcttctttaa atattttata tgatattata aaattaaata acattgcgaa
     1021 aacatatgaa atcatcaaaa taccaaatat atagtgagaa tgcattatta tttagctcct
     1081 tcttttggct ctgacaaatt gacagtccat tgatatccaa tgcttgcatt tgcaattgct
     1141 gacgcacctg cccctgtttt ccctgttagt atcaatggtt cttttcttgc aagatgtgca
     1201 cctgcacaac cacctaatac taaaccacca cctattgtaa atccttcctg ttgtatctct
     1261 aatgataaca ttttcttctt gtgccgccct taatacagat tgtggaattg gattgacctg
     1321 agcaggaact tctaaaataa gttttcctgt aagttttctt cctcttagcg gagcaggtgt
     1381 acttggaaca ttggaaatga caaagtgagt acgcaaacca cctgaaggcg atgtgtttga
     1441 acctgagacc tttgcaaaaa tagtctgtta acgaaatttg acgcataaaa atgcgccaaa
     1501 aaattttcaa tt
//
LOCUS       DAXHKX010000254         1440 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXHKX010000254 DAXHKX010000000
VERSION     DAXHKX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.254"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            26..1363
                     /locus_tag="ACFMWZ_002089"
     CDS             26..1363
                     /locus_tag="ACFMWZ_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC5935978.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYLISAVIMPVGALSVA
                     VFTAWIQDRQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          261 a          339 c          389 g          451 t
ORIGIN      
        1 atcttcacat ttcttaataa attttatgag taaccatact tcttggtcgt ccaaaatcgg
       61 tttcgtgctt gctgcggcag gttcggccat cggtttgggc gcgatttgga aatttcctta
      121 tacggcaggc accaacggcg gcgcggtgtt tttcctgctg tttttgatat ttaccgtctt
      181 ggtcgccctg cccgtccagc ttgccgagtt ttatatcggg cgcacaggcg gtaaaaatgc
      241 cgtcgattcc ttcagggctc tgcgtccgag tacgcaatgg ctttgggtcg ggcgcatggg
      301 cgtttccgcc tgctttattt tgctgtcgtt ttacagcgtg gtcggcggat gggtattaaa
      361 ttatgtcgtc cacagtttca cgggggcggt tcataccggc gcggactttg aagccttgtt
      421 cggcgcgacg atttccaatc cggcaggttc gctgtcctat caggcactgt ttatgctgat
      481 tacggtttgg gtggtcaaag gcggcatttc agacggcatt gaaaaggcaa accgttatct
      541 gatgccgggg ctgtttatcc tctttattgc gctggcaatc cgttcgctga cgctgccggg
      601 tgcaatggag ggcgtgtctt tcctgctcaa accgaattgg tcgtacttta aagccgatac
      661 gatgattacg gctttaggtc aggcgttttt tgccttgagc atcggcgttt ccgctatgat
      721 tacctatgct tcgtatttgg gaaaagatca ggatatgttc cgctcggggc atacgattat
      781 gtggatgaac ctcttggttt cgctgttggc aggtttggtg atttttccgg cggtgttcgc
      841 cttcggtttt gaaccgagcc aggggccggg attgattttt atcgtattgc cggcggtgtt
      901 tatgaaaatg ccgttcggta cggttttgtt tgcggtattt atgctgctgg tcgttttcgc
      961 cacgctgact tcggcatttt cgatgttgga aacggtcatc gccgcaacca tccgccaaga
     1021 cgagcgcaaa cgcaaaaaac atacttggct tatcggcacg gctattttta ttgtcggcat
     1081 cccgtccgcg ctgtctttcg gcgtatgggg tgagtttaag gttttcggca aaaccatttt
     1141 tgatttgtgg gactacctta tttctgcagt catcatgcct gtcggcgcat tgagcgttgc
     1201 cgtatttacc gcttggattc aggacaggca gtctgtgtta aaagatgccg gcgcgggcag
     1261 caccgtacca cgggcagtgt tgctgctgtg gctgaatacc ttgcgctacc tcgccccgat
     1321 tgccattatt attgttttca tcaattcttt ggacatcctt taaaagccat ccaaacagca
     1381 aaaatgccgt ctgaaagcct ttcagacggc atttttgctt caggttcagc ctatttcgtt
//
LOCUS       DAXHKX010000255         1416 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXHKX010000255 DAXHKX010000000
VERSION     DAXHKX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.255"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            81..446
                     /locus_tag="ACFMWZ_002090"
                     /pseudo
     CDS             81..446
                     /locus_tag="ACFMWZ_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            913..>1416
                     /locus_tag="ACFMWZ_002091"
     CDS             913..>1416
                     /locus_tag="ACFMWZ_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC5935979.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGKWPGGNSSAGVASASDIKGKYVEKVEVKNGVVTATMLSSG
                     VNNEIKGKKLSLWAKRQDGSVKWFCGQPVTRDNAKATGANADDVTKTGTDNEKINTKH
                     LPSTCRDA"
BASE COUNT          474 a          398 c          266 g          278 t
ORIGIN      
        1 ctcccgataa attcctaaaa cttaaaattt catcattccc acaaggacag aaaaccaaaa
       61 acagaaacct aaaattcgtc attcttttgg ctgaaggtca aaaatcagcc gttaccgagt
      121 attacctgaa tcacggcgaa tggcccggcg acaacagttc tgccggcgtg gcatcctcct
      181 ctaaaatcaa aggaaaatat gttaaggaag ttaaagtcgc aaacggcgtc attaccgcca
      241 caatgctttc aagcggcgta aacaaagaaa tcaaaggcaa aaaactctcc ctatgggcca
      301 agcgtcaaga cggttcggta aaatggttct gcggacagcc ggttgcgcgc gacaaagccg
      361 acagcgccac cgaagtcacc gccgacggca acgacaacat caacaccaag cacctgccgt
      421 caacctgccg cgacgcagca tctgccgttt gcatagaaac accacgcgcc gatttcaaac
      481 actttccaag aaaacggaaa tttttaaaaa ttaaatttaa aatttcccac ccacacccac
      541 cctatgctac cgcgcaaatt caaaaatctc aaattccgac ccaatcaaca cacccgatgc
      601 cccatgccaa tagaaatacc ccacgccaat aaaaaagtgc cgaaaatcgg cactaaaact
      661 gacaattttc ggcattactg acaaaaaacg gtaaccccct gcttcctcaa accacgccca
      721 aacgaaagaa aatgtaaaat aaaaacaacc atataaagat aaacgcataa attttcacct
      781 caaaacacaa acttggtacc aatcttgctt tataatacac aggtatcgta acaaaatacc
      841 gatggtcaaa tacattgcat aatgccgatg gcgtaagctt gaagcatttc ctttccaatt
      901 aggagtaatt ttatgaacac ccttcaaaaa ggttttaccc ttatcgagct gatgattgtg
      961 attgccatcg tcggcatttt agcggcagtc gcccttcctg cttatcaaga ctacacagcc
     1021 cgcgcacaag tttccgaagc cattctttta gccgaaggtc aaaaatcagc cgtcaccgag
     1081 tattacctga atcacggcaa atggcccggc ggcaacagtt ctgccggcgt ggcatccgcc
     1141 tccgacatta aaggcaaata tgttgagaaa gttgaagtca aaaacggcgt cgttaccgcc
     1201 acaatgcttt caagcggcgt aaacaacgaa atcaaaggca aaaaactctc cctgtgggcc
     1261 aagcgtcaag acggttcggt aaaatggttc tgcggacagc cggttacgcg cgacaacgcc
     1321 aaagccaccg gcgccaacgc cgacgacgtc accaagaccg gtaccgacaa cgaaaaaatc
     1381 aacaccaagc acctgccgtc aacctgccgc gacgca
//
LOCUS       DAXHKX010000256         1413 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXHKX010000256 DAXHKX010000000
VERSION     DAXHKX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.256"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..209
                     /locus_tag="ACFMWZ_002092"
     CDS             <1..209
                     /locus_tag="ACFMWZ_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935980.1"
                     /translation="VDVFGNHGLGGLPEEGAHEKSPKCLGGNLGDFGEFCKGLILQDF
                     SLIIKQGTAKIPAATGIFVRLSPV"
     repeat_region   496..1389
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=562..597
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
BASE COUNT          320 a          332 c          301 g          460 t
ORIGIN      
        1 cggttgatgt gtttggcaat catggcttgg gcggtttgcc ggaagaaggt gctcatgaga
       61 aatctcctaa atgtcttggt gggaatttag gggattttgg ggaattttgc aaaggtctca
      121 ttttgcaaga ttttagttta atcatcaaac agggaacagc aaaaatcccc gctgcaacgg
      181 ggatttttgt tcggctctcg cctgtgtgat taacttcact aggagaagct aaaatgctta
      241 aattccttat gcgtgggaaa aagttgctgc tgtccgttga agtgaaccac aagttcattt
      301 tagcgattgc tttgctgtta agtcaatgca gtagctaacc cgaccacaat caaaaaaacg
      361 caagttttcc gcctactctg tgagtgggcg gttttttctt atttcaaatc tgtgacacaa
      421 tatctgtcgc ctttcttgcc ataattgcac tgtgtctcaa tgactttaac gcacgttcgc
      481 ttatcgcaac ggctggttgt agcgcccatt ctcatttcgc agtgctacaa tttctattat
      541 aaaagctgcc tgacttactc ggttgtagct ccctttctca tttcgcagtg ctacaatagc
      601 atttgggcgg cacgggcaaa gccgtcagtt gtagctccct ttctcatttc gcagtgctac
      661 aattaaacct ctgtcggttg gtttgcttag attgttgtag ctccctttct catttcgcag
      721 tgctacaatc gaattggaaa agttgcccta ccggtggggg ttgtagctcc ctttctcatt
      781 tcgcagtgct acaatgtcgc atggtaaaat aaccaccgct ttacagttgt agctcccttt
      841 ctcatttcgc agtgctacaa taaactaaaa ctttggaaag ttggaaagtt ggttgtagct
      901 ccctttctca tttcgcagtg ctacaatcgg cgcatcccct ttaatttaat atcacttgtt
      961 gtagctccca ttctcatttc gcagtgctac aatatagaac ctatggctgc tacggcgttg
     1021 acagttgtag ctcccattct catttcgcag tgctacaatt tttgtggagt tttgttatga
     1081 gtgagggcag ttgtagctcc ctttctcatt tcgcagtgct acaatctgca agcattttca
     1141 tcatcggctg tcatagttgt agctcccttt ctcatttcgc agtgctacaa tctgcaagca
     1201 ttttcatcat cggctgtcgt agttgtagct ccctttctca tttcgcagtg ctacaatgga
     1261 agagcggcat ctgaatgcgg aagaagagtt gtagctccct ttctcatttc gcagtgctac
     1321 aatcatcaaa tcatccgccg cgattcgatc gcagttgtag ctccctttct catttcgcag
     1381 tgctacaata tgatgccgaa accctgcaag ccc
//
LOCUS       DAXHKX010000257         1383 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXHKX010000257 DAXHKX010000000
VERSION     DAXHKX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.257"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..330
                     /locus_tag="ACFMWZ_002093"
     CDS             <1..330
                     /locus_tag="ACFMWZ_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC5935981.1"
                     /translation="IIATTVPAQQTFLDAAELMQWSIETEGLGLNVISHKILGKDHAQ
                     VEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCG
                     KYLKPVA"
     gene            complement(547..1341)
                     /locus_tag="ACFMWZ_002094"
     CDS             complement(547..1341)
                     /locus_tag="ACFMWZ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC5935982.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          363 a          420 c          313 g          287 t
ORIGIN      
        1 atcatcgcta ctaccgttcc ggcgcaacaa acattcctcg atgccgccga actgatgcaa
       61 tggagtatag aaaccgaagg gctgggcttg aacgtcatct cgcacaagat actcggcaaa
      121 gaccacgccc aagtcgaatt tgaagcctac ttccgagacg gacaacaccg atccgcgcat
      181 cacgaactgt ccggcttcgt caacatcggc ggacaatggt attttatcga tcctaccgtt
      241 ccgcatcctg cgatgaaaca accctgcatt tgcggatcgg gcaaaaaatt caaagcctgc
      301 tgcggcaaat atctgaaacc tgtcgcataa aaatgccgtc tgaacgttca gacggcattt
      361 tcaacacgca aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
      421 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg gcagcggtaa tcagcggaaa
      481 ggattgtacc cgaattaatg ttaagaaacg ttaatcgtga aaatatatta acaaacctgt
      541 tgaaacctat tggttttccc gtatccaccc gacccagcgt tcaaacagct tcggttcgag
      601 cgcggcaacg accgagcgtt tgaacacgtg ttcaccactc caaaacccgt cgccttccaa
      661 agtcgtcagc ctgccgcccg cctcctcgaa aatcagcgca ccggcggcat aatcccacag
      721 cttctgcccg ccgtggacat aaacatcata acgcccgcac gccagataac accaatccaa
      781 cgtactgctg cccatactgc gtatcgtccc gaaaggcgcg agcgtactca tgcggctgga
      841 aagtttgccc gaacgcagat atttgatttc cacgcccgca atcgcctcat tgagtttttt
      901 atccacgagg cgcaggggca ggcgcgtccc gtttaaaaac gccccctgcc cccgttcggc
      961 ataaaaacat tcgccgctga ccgggttgta gattacgccc aattcggcgc gcccgttgcg
     1021 gacaaacgcc accgacacgg caaaatgcgg cagcccgttg acaaaattgt tcgtcccgtc
     1081 aatcggatcg acaatccaca gccccttttc ccccgaatat tgttcccaca aagccgactg
     1141 ttcctgccgc gacatttcct cgcccaacat cgggcaatcg atcagaagcg gcaacgcggc
     1201 agcaaaagcc gtctgcgcgg caatgtccgc ctcgctcaac atcgaaccgt cttccttgcg
     1261 gtgggacggc gtattcaaaa aacgcggcat aatttcggtt tgcgcgatat ggcgcacgac
     1321 tttctgcaaa cggtgtaaca cttcctactg tcctcatatt ttgaacttgc ggcgcgcgaa
     1381 cgt
//
LOCUS       DAXHKX010000258         1355 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXHKX010000258 DAXHKX010000000
VERSION     DAXHKX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.258"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..>1355
                     /locus_tag="ACFMWZ_002095"
     CDS             <1..>1355
                     /locus_tag="ACFMWZ_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC5935983.1"
                     /translation="VATKGFAGALFDHRNQGIRTATGWVAADDGLLVRDLNGNGIIDN
                     GAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLNQDGISQ
                     ANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTNGTTAKMGDLLLAADNL
                     HSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQLAL
                     LDNLIHKWAETDSEWGKKPPMHLSTSWTQTANGGVALTPSQVTRLKQNALVSLSDKAK
                     AAIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLFQT
                     RLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYE
                     GRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNVSL
                     YG"
BASE COUNT          393 a          321 c          320 g          321 t
ORIGIN      
        1 gttgccacca aaggctttgc aggtgcattg ttcgaccacc gcaatcaagg catccgcacc
       61 gccaccggtt gggttgctgc cgatgatggt ttgcttgtgc gcgatttgaa cggcaacggc
      121 atcatcgaca acggtgcgga actcttcggc gacaacacca aactggcaga cggttctttt
      181 gccaaacacg gctacgcggc tttggccgaa ttggattcaa acggcgacaa catcatcaac
      241 gcggcagacg ccgcattcca aaccctgcgt gtatggcagg atctcaacca ggacggcatt
      301 tcccaagcta atgaattgcg tacccttgaa gaattgggta ttcaatcttt ggatctcgcc
      361 tataaagatg taaataaaaa tctcggtaac ggtaacactt tggctcagca aggtagctac
      421 accaaaacaa acggtacaac cgcaaaaatg ggggatttac ttttagcagc cgacaatctg
      481 cacagccgct tcaaagacaa agtggaactc actgccgaac aggcaaaagc cgccaatctt
      541 gcgggcattg gtcgtctgcg cgatttgcgc gaagctgccg cattgtccgg tgatttggcc
      601 aatatgctga aagcttattc tgccgccgaa actaaagaag cacagttggc attgttagat
      661 aatttgattc acaaatgggc ggaaaccgat tcggaatggg gcaaaaaacc gccgatgcat
      721 ctttcaacca gttggacgca aacggctaac gggggtgtgg ctcttacccc gtctcaagta
      781 acgcgactaa aacagaacgc tttagtttct ctttccgaca aagccaaagc agctatcgat
      841 gccgcccgcg accgcattgc cgtgcttgat gcctacacgg ggcaggattc cagcacactc
      901 tattacatga gcgaagaaga cgcgcttaat atcgtcaaag taaccaacga tacatacgac
      961 catctcgcca aaaacatcta ccaaaacctg ttgttccaaa cccgtttgca gccatatttg
     1021 aatcaaatca gtttcaaaat ggaaaatgat acgttcactt tggattttag tggtcttgtt
     1081 caagcattta accatgtcaa agaaactaat ccgcaaaaag cttttgtgga tttggccgag
     1141 atgcttgcat atggcgaact tcgttcttgg tatgaaggtc gaagactaat ggccgattat
     1201 gtggaggagg caaaaaaagc aggtaaattt gaagattacc agaaagtgtt gggtcaggag
     1261 accgttgcat tattagctaa aacatcgggt acgcaagcag atgatatcct gcaaaatgta
     1321 ggctttggtc ataataaaaa tgtttcttta tatgg
//
LOCUS       DAXHKX010000259         1344 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXHKX010000259 DAXHKX010000000
VERSION     DAXHKX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.259"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..1170
                     /gene="lpxB"
                     /locus_tag="ACFMWZ_002096"
     CDS             <1..1170
                     /gene="lpxB"
                     /locus_tag="ACFMWZ_002096"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC5935984.1"
                     /translation="WGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARFV
                     GIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVGI
                     DAPDFNLGVAERLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYL
                     DAGGRAEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFF
                     QTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLPLTVTDRQSETVCRAADA
                     VLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELL
                     QSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            1178..1336
                     /locus_tag="ACFMWZ_002097"
     CDS             1178..1336
                     /locus_tag="ACFMWZ_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935985.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGQLTD"
BASE COUNT          292 a          301 c          439 g          312 t
ORIGIN      
        1 tggggacaga cgaatatggt tgataaaaaa agccctttga ttgccgtcag tgtcggcgaa
       61 gcgtcgggcg acctgttggg ggcgcacctg atacgcgcca tccgcaagcg ttgtccgcag
      121 gcgcgttttg tcggtatcgg cggcgaactg atgaaggcgg aaggtttcga gagcctttat
      181 gatcaggagc ggctggcggt gcgcggcttt gtcgaagtgg tcaggcggct gccggaaatt
      241 ttacggatac gcagggggct ggtacgggat ttgctgtcgt tgaaacctga tgtctttgtc
      301 ggtatcgatg cgcccgattt taatctgggt gtggcggagc ggctcaaacg gtcggggatt
      361 ccgaccgtgc attatgtcag tccgtcggtt tgggcgtggc ggcgggaacg cgtgggcaaa
      421 atcgtgcatc aggtcaaccg cgtgctgtgc ctgttcccga tggagccgca gctttatctt
      481 gatgcgggcg gacgtgcgga gtttgtcggt catccgatgg cgcagcttat gcccttggaa
      541 gacgaccgtg aaacggcgcg gaaaactttg ggcgtggatg ccggcatccc cgtattcgcc
      601 ctgctgcccg gcagccgcgt cagcgaaatc gactatatgg cgccggtgtt ttttcagacg
      661 gcattattgt tgttggaacg ctatcccgcc gcacgcttcc tgctgcctgc cgcaactgag
      721 gcgacgaagc ggcgtttggc ggaagttttg cagcgtccgg agtttgccgg attgccgctg
      781 acggtaaccg acagacagtc tgaaacagtg tgcagggcgg cggatgcggt gctggtaacg
      841 agcggtacgg caactttgga ggtggcgttg tgtaagcgtc cgatggtcat cagctacaag
      901 atttcgccgc tgacctatgc ttatgtgaaa cgcaaaatca aagtgccgca tgtcggcctg
      961 ccgaatatcc tgttgggtaa ggaggctgtg ccggaattat tgcaatctga agcaaaaccg
     1021 gaaaaactgg cggcggcgtt ggcggactgg tacgaacacc ccgataaggt tgccgcgctg
     1081 caacaggatt tcagggcgtt gcacctgctg ttgaaaaaag atacggcgga tttggccgcg
     1141 cgcgcggttt tggaagaggc gggatgttga gcggttaatg gattattttc ccgaagcagc
     1201 acgtattaca aaaaaagggg gagaaattgt gattaatggc acatcaaaca ataagtattt
     1261 aagaggaatt ccaaatgaaa cagaactggc ccgaatggga ttaaggttaa aatataatgg
     1321 tcagttaact gattaatttt gtta
//
LOCUS       DAXHKX010000260         1319 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXHKX010000260 DAXHKX010000000
VERSION     DAXHKX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.260"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="ACFMWZ_002098"
     CDS             complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="ACFMWZ_002098"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC5935986.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
BASE COUNT          342 a          324 c          302 g          351 t
ORIGIN      
        1 gaacaataac cgatcctacc cgctgttttt cccatcatac aacatacaaa tgccgtctga
       61 aacatccggc ttcagacggc attttgtcaa aaaacgttta caactcaaaa tcgcccaaat
      121 catccgtatt cacttcagaa tcgatctgac cgatcaaata agaggatatt tccacttcct
      181 gcggcgcgac ctgtacgttg tcggacgaca gccacgcatt aatccacgga atcgggtttt
      241 gatttgcgcc ttcaaatccg gccggcagcc ccaccgcctg catacgcaga ttggtaatat
      301 attcgacgta ttgggataag atttctttgt tcaaaccgat catcgaaccg tccttaaaca
      361 aatatgccgc ccactctttt tcctgttccg ccgctttttt gaagagttgg aaacattcgt
      421 cctgcaactc ggcggcaatt tctgccattt cagaatcatc gacacccgaa cgcatcagat
      481 taagcatatg ctgcgtgccg gtcaggtgca gggcttcgtc gcgggcaatc agtttgatga
      541 ttttggcgtt gccttccatc aactcgcgct cggcaaaagc aaacgagcag gcgaatgaaa
      601 cgtagaaacg gatggcttcc aacacattga cgcacatcag gcagagatag agttttttct
      661 tcaacccgcg caaagacacg gtaacgggtt tgccgccgac attgtgcacc ccttcgccca
      721 acaggttgta atactgggtg tattcgatta agtcatcgta atagcaggca atgtcttcgg
      781 cgcgggcggt aatgtattcg ttttcgacaa tatcatcaaa cacgaccgac ggatcattca
      841 caatattgcg gatgatgtgg gtatagctgc gcgagtggat ggtttcgctg aagctccacg
      901 tttcaatcca cgtttccaac tcgggaatcg aaaccaaagg cagcaaagca acattcggac
      961 tgcgcccttg gatggaatcg agcagtgttt ggtatttcag attgctgatg aaaatatgtt
     1021 tttcgtgttc aggcaggttg gcgtagtcga tacggtcgcg cgacacgtcg atttcttccg
     1081 gccgccaaaa aaacgacaat tgtttttcaa tcagtttttc aaatacctcg tatttctgct
     1141 ggtcataacg ggcaacatta accggctgac cgaaaaacat cggctctttc agcgcgtcgt
     1201 ttttggtttt gggaaaggtg ctgtatgaca tgactaggtg ttcgcaggac atagctgttt
     1261 tctcaggttg atttaaatct aaaagtagga taggcgactt ctaggctatg caaagcata
//
LOCUS       DAXHKX010000261         1294 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXHKX010000261 DAXHKX010000000
VERSION     DAXHKX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.261"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..890
                     /locus_tag="ACFMWZ_002099"
     CDS             <1..890
                     /locus_tag="ACFMWZ_002099"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC5935987.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          354 a          381 c          330 g          229 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttg
      121 ccgaagccaa agccaacctg cccaaagacg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaatcccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgcgtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagccgac agcgaaaacg ctgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctggcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctagaaacg aaaagtaaca
     1021 ggaatttatc gggaatggct ggaatttaaa ggtctggatt cccgctttcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa acctatgccg tcattcccga
     1141 gcaggcggga atccggttat ttaaaactgc agaaatttat ccgaagcaac aacaatcttt
     1201 ccatcgtcat tcccgcgtag gcgggaatct agcaacgaaa agtaacagga atttatcggg
     1261 aaaacagaaa cctctatgcc gtcattcccg cgaa
//
LOCUS       DAXHKX010000262         1270 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXHKX010000262 DAXHKX010000000
VERSION     DAXHKX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.262"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(616..900)
                     /locus_tag="ACFMWZ_002100"
     CDS             complement(616..900)
                     /locus_tag="ACFMWZ_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC5935988.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          335 a          330 c          243 g          362 t
ORIGIN      
        1 cgataaatgc cgcaatctca aatcccgtca ttcccacgaa cctacattcc gtcattccca
       61 cgaaagtggg aatccagaat ctctaaagct tcagctaacc tttgaatatt gctgttgttc
      121 taaggtctaa agcttcagct aacctttgaa tattactgtt gttctaaggt ctagattccc
      181 gcctgcgcgg gaatgacggc ggagcggttg ctgttttttc cgataaatgc cgcaatctca
      241 aatcccgtca ttcccacgaa cctacatccc gtcattccca cgaaagtggg aatctaggtc
      301 tgtcggcaca gaaacttatc gggtaaaacg gtttctttag attttacgtt ctagattccc
      361 gcctgcgcgg gaatgacgaa tccatccgca cggaaacctg caccgcgtca ttcccacgaa
      421 cctacattcc gtcattccca cgaaagtggg aatctagaat ctctaaagct tcagctaacc
      481 tttgaatatt actgttgttc taaggtctgg attcccgcct gcgcgggaat gacggtttag
      541 aagttgcccg aaacctcaaa aaaaaaaaaa ccgaaaccgt acaagccgga ttcccgcctg
      601 cgcgggaatg acgggctaaa taatatcaaa ccataaatcc tgccaagaaa cattattttc
      661 ttcaatcagt tgcaatttcc aagccctgtt ccatttcttc aactgttttt cccgagtaat
      721 tgcgctctcc atcgtaggat gcagttcata ccaaaccagc atagtaacgt tgtaccgtga
      781 tgtaaatccc tcaatcaaat gctccctatg ttggtaaata cgttgcacca aatcagatgt
      841 aacgccaatg tataacgtgc cattacgttg gcttgctaaa atataaaccg caggctgcat
      901 ataataccct tttgaattat ttaaatttat attcccgcga acaccatccc gtgattactt
      961 taacctttcg ttattcccat agctttccat cattcccgca actcttcgtc attcccacga
     1021 aagtgggaat ctagaatctc taaagcttca gctaaccttt gaatattact gttgttctaa
     1081 ggtctagatt cccgcctgcg cgggaataac gggtctttta taaactttga atattgccgt
     1141 tatcccaagg tctagattcc cgcctacgcg ggaatgacgg catcggtctg ccgttacaac
     1201 acggtttctt tagattttac gttctggatt cccgcctgcg cgggaatgac ggcggagcgg
     1261 ttgctgtttt
//
LOCUS       DAXHKX010000263         1260 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXHKX010000263 DAXHKX010000000
VERSION     DAXHKX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.263"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(1..317)
                     /locus_tag="ACFMWZ_002101"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACFMWZ_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(427..849)
                     /locus_tag="ACFMWZ_002102"
                     /pseudo
     CDS             complement(427..849)
                     /locus_tag="ACFMWZ_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          252 a          264 c          350 g          394 t
ORIGIN      
        1 ttgtcggtgg tgtcggcttt gacgtcggtg gcggtggcgt cgcgcgtaac cggctgtccg
       61 cagaaccatt ttaccgaacc gttttgacgc ttggcccaca gggagagttt tttgcctttg
      121 atttcgttgt ttacgttgct tgaagccatt tgggcggtaa tgacgccgtt tgcgactgta
      181 acgctttgaa catatttgcc tttgatttca gaggaggttg ccacgccggc agaattgttg
      241 tcgccgggcc atatgccgtg attcaggtaa tactcggtga cggctgattt ttgaccttca
      301 gccaaaagaa tggcttcgtc attcccgcgc aggcgggaat ctagcctcgt cggcacggaa
      361 atttatcggg aaaacggttt ctttagattt tgcgttctgg attcccactt tcgtgggaat
      421 gacggtttag gtatttttat agaaagccgt aggtggtgtt tctatgcaac cggcagttga
      481 ttcattgcgg caggttgacg gcaggtgctt ggtgtcgatg ttggcggtgc cggtgccgct
      541 ggcggcggtg acttcgtcgt tggtggtggt ggtgccggtg cgcgtaaccg gctgtccgca
      601 gaaccatttt accgaaccgt cttgacgctt ggcccacagg gagagttttt tgcctttgat
      661 ttcgttgttt acgttgcttg aagccattgt ggcggtaatg acgccgtttg cgactgtaac
      721 ttccttaaca tatttgcctt tgattgttga agaagatgcc acgccggcat cactgttgcc
      781 gccgggccat ttgccgtgat tcaggtaata ctcggtgacg gctgattttt gaccttcggc
      841 taaaagaatg acgaatttta ggtttctgtt tttggttttc tgtccttgtg ggaatgatga
      901 aattttaagt tttaggaatt tatcgaaaaa acagaaaccg ctccgccgtc attcccgcgc
      961 aggcgggaat ccagcctcgt cggtgcggaa acttatcggg taaaacggtt tcttgagatt
     1021 ttacgttctg gattcccact ttcgtgggaa tgacgggatt taagtttcaa aatttattct
     1081 aaataactga aacttaacga actagattcc cactttcgtg ggaatgacga attttaggtt
     1141 tctgtttgtg gttttctgtt tttgtaggaa tgatgaaatt ttaagtttga ggaatttatc
     1201 ggaaaaaaca gaaaccgttc cgccgtcatt cccgcgcagg cgggaatcca gcctcgtcgg
//
LOCUS       DAXHKX010000264         1254 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXHKX010000264 DAXHKX010000000
VERSION     DAXHKX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.264"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            237..1190
                     /locus_tag="ACFMWZ_002103"
     CDS             237..1190
                     /locus_tag="ACFMWZ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC5935989.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          200 a          272 c          432 g          350 t
ORIGIN      
        1 caaaccggat gattcgggct aaataattcc tgattctttg gatatagcca acggtatgcc
       61 tgccgatgcc gtcagaaagg gcttgatggg cgtttgcggt tcgggttaat atttgtgctg
      121 tatctgttaa ttccggttag taaaatgggg cggggacgat gagtggccgt cggtcggttt
      181 tgtacgggcg gtaataatgt ccggcgcaag gtgcgccgtt tcgggagggg tgtgtgatgg
      241 gttttctgat tggtttgagc cgcaacctga cgcggtttac ggctttggtc attttgcttg
      301 cgtcggcggt tgcgtttatc ggaccggctt cgtttgcgtg ggttaagggc gatgttcagg
      361 tcttggtgct gggcatcatt atgctgggta tggggatgac tttgggcagg gaggactacc
      421 ggattttggc gcagcgtcct ttggatgtgt ttatcggtgc ggcggcgcaa tataccgtga
      481 tgccgttgct cgccatcggt attgccaagc tgttcgacct ttcgccgggg ctgactttgg
      541 ggctggtgtt ggtggggacg tgtccgggcg gcgtgtcgtc caacattatg agtttccttg
      601 ccaaaggcga tgtggcgttt tcagtcggta tgacgacggt gtctacggtg ctggctccgg
      661 ccgttacgcc tttgtggatg acctgcctgg tcggtcagac ggtggatatg gacggttggg
      721 ggatgttccg gtttatgctg ctggtcacgc ttttgccggt ggtgctgggt tcggcggcaa
      781 atatcctgct gcacggaaaa cattggtttg aaaatgtccg tgccgttatg ccgggcgtgg
      841 cggtattggc atttgcctgc attgtcggcg gcgtggcttc ggttcacgga caccgttttg
      901 ccgaatcggc gttggtaatg gtgttggcga ttgcggtaca caatatcgcc ggctatgtgt
      961 caggctatta cgccgccgcg ctgtttggta tggatacggc aaaaaaacgc accttggcaa
     1021 tcgaagtcgg cgtgcagaat gccggtttgg caacaggatt gagcgcgaaa tttttcccag
     1081 gcaaccccga atcggcggtt gcggcggcgg tcgcctgcgt gtggcattcg gtttcgggga
     1141 cggtattggg caacttgttc gctctgtggg acaagcgtcg ggacgcttag tcgggcgtgt
     1201 ccgaaccgtc ccacgatata tagtggatta actttaaacc ggtacggcgt tgcc
//
LOCUS       DAXHKX010000265         1217 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXHKX010000265 DAXHKX010000000
VERSION     DAXHKX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.265"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            111..>1217
                     /gene="pilC"
                     /locus_tag="ACFMWZ_002104"
     CDS             111..>1217
                     /gene="pilC"
                     /locus_tag="ACFMWZ_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC5935990.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGAQAQTYSYALVMNGQ
                     QLPEVKWGNSYNQLTHKNNTRQATFHSYFQGARKKTTFSFNNTDEVVAQKGDTVVFGA
                     ATYLPPYGKVSGFDERKLKERTNALNWINTTRPGLVGYHYQDSTCSSGNCPELSYKTQ
                     FTFGHSSLAKKTNGGKLDIYEDKSRDGSPIYKLQGYSWLGVSFNLSGESTAESKKLKN
                     LVSSFSEEVTQNNGADSQHKDKNLVYTTGDGRNNNKTHQDKHHAVAFYLNAKLHLLDK
                     QQIKNITQGSELNLGVLKTRIEPTDAWKNKRHLVINADWEFKDTGIVSVKLKLPEVKA
                     GRCINKPNPNKNSKALSPALTAPALWFGPVQNGKV"
BASE COUNT          371 a          299 c          286 g          261 t
ORIGIN      
        1 gtaagctttt caatcagcaa ttcggcgggc gcggaatcgg gcggtttacc gaacctcggc
       61 gttcgcggcg cgccgccccg tcccgtgaag gcaaactcaa ggaataaaag atgaataaaa
      121 cttggaaacg gcaggttttc cgccataccg cgctttatac cgccatattg atgttttccc
      181 ataccggcgg ggggggggcg caagcgcaaa cgtattcata cgctcttgta atgaacggac
      241 aacagctgcc tgaggtaaag tgggggaatt catataatca attgacgcac aaaaacaata
      301 cccgccaagc tacctttcat tcatattttc agggagctag aaaaaaaact actttctcat
      361 tcaataatac cgatgaagtt gttgctcaaa aaggcgatac tgtcgttttc ggcgcggcga
      421 cctacctgcc gccctacggc aaggtttccg gttttgatga aagaaagctg aaagagcgca
      481 ccaatgccct taattggatt aatacgactc gcccggggtt ggtaggctac cactaccaag
      541 atagcacatg ctccagcgga aactgtcctg aacttagcta taaaacccaa tttaccttcg
      601 gtcattccag tttggcaaaa aagacaaacg gcggcaagct ggatatatac gaagacaaaa
      661 gccgcgacgg ttcgcccatt tacaaattgc aaggttattc ttggttgggc gtgtctttca
      721 atttgagcgg cgaaagcacc gccgaatcaa agaaactcaa gaacttggta tcttctttta
      781 gcgaggaagt aacgcagaac aatggtgcgg acagccaaca caaagacaaa aacctcgtct
      841 atacgacagg cgatggccgt aataataata aaacccatca ggacaaacac cacgccgtcg
      901 ccttttatct gaacgccaaa ctgcacctgc tggataaaca acaaatcaaa aatatcacgc
      961 aaggcagtga acttaatttg ggtgtcttga aaacgcgcat cgagccgacg gacgcgtgga
     1021 agaataagag acatctcgtt attaatgctg attgggagtt taaagatacc ggaatagtca
     1081 gcgtcaaact caaattgccg gaagtcaaag caggccgctg catcaataaa ccgaacccca
     1141 ataaaaacag caaagccctt tcgccggcac tgactgcccc cgcgctgtgg ttcggacctg
     1201 tgcaaaatgg taaggtg
//
LOCUS       DAXHKX010000266         1202 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXHKX010000266 DAXHKX010000000
VERSION     DAXHKX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.266"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            275..543
                     /locus_tag="ACFMWZ_002105"
                     /pseudo
     CDS             275..543
                     /locus_tag="ACFMWZ_002105"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(633..1082)
                     /locus_tag="ACFMWZ_002106"
     CDS             complement(633..1082)
                     /locus_tag="ACFMWZ_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC5935991.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYIEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          399 a          262 c          179 g          362 t
ORIGIN      
        1 ttatgtctaa aggtgcaaac atgccttaaa ttaccatagt ggattaaatt taaatcagga
       61 caaggcgagg caacgctgta ctggtttaaa tttaatccac tatatattgc ataggcaaaa
      121 tacaagccta taacgaattg gaaacaaaat gccgtctgaa aacatcttca gacggcatta
      181 taaaatctgt tcaccttttc agatgagtaa tgtacaccct tatacaattt ttgctactat
      241 gccccataaa tccacggcta aagatatcct tattatgtcc tatgatttat cgaaacgact
      301 tgtaatcggc ttagcatcaa gtgccctatt cgacttatcc gaatcggata atatatttag
      361 aatggaaggg gcagaaacct ataggcaata tcagagggaa aaacaaaacc atcccctaaa
      421 aaaggcgttg tctttccatt tattaaaaaa cttctgtcaa tcaatgaaat aaacccaaac
      481 gacccaacga ttgggtttat tcttttatcc agaaacaatc cagatacaga ttacgagtca
      541 taactatagg cttaatatta cacgattttc attccatcaa ggcggaaaac cgcacaaata
      601 ctgaaacact atcgatcgat ttgtaaacaa gcctacttaa gtaacttgca gtccttatca
      661 tttcctttaa aataatccag cccgtcacta cacgaactgg cggacttctt gcaaataaag
      721 gttactagat tttcattcat cttaataata aaaggatttt tatctttatc tatggctacc
      781 gccttcaaca tgaatttact gtctaaggcc ccgcgcgcga ttccattcaa acggatacaa
      841 aagccttctg cctctttaat cggcaaactt ggccatttgg tagatgtttg tttaaatctc
      901 ccattctgca gataaaactt ttccataaaa tgtgcatttt ctaacaaggc tgcccgcact
      961 gcatttatct ttgctttctc aatataattg cgatagctcg gataaacaat taaagcaagt
     1021 acagacaata tcaagaccac tgatattaat tcaaccagcg taaacccccg attatcagtc
     1081 attactttac ttccaataag aacagattat tcaacatatt tctttgaaca gacttactat
     1141 cccattcaac agtatgcata tttcccactc tattttttag cggccggtat agccggtttg
     1201 gc
//
LOCUS       DAXHKX010000267         1128 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXHKX010000267 DAXHKX010000000
VERSION     DAXHKX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.267"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(231..1085)
                     /locus_tag="ACFMWZ_002107"
     CDS             complement(231..1085)
                     /locus_tag="ACFMWZ_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC5935992.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCSW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSVLGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          312 a          337 c          295 g          184 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cgggaatccg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgatg caaagatggt
      121 cgttatttcg gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caagacggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacgttggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagctgca ccccacgccc aaatacagca gcgacaatac cattccgacg
      481 ctccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagtgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cagcaatcag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tagcggat
//
LOCUS       DAXHKX010000268         1113 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXHKX010000268 DAXHKX010000000
VERSION     DAXHKX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.268"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            154..1053
                     /locus_tag="ACFMWZ_002108"
     CDS             154..1053
                     /locus_tag="ACFMWZ_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC5935993.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          187 a          254 c          356 g          316 t
ORIGIN      
        1 cgggttttat ctggcaggac ggttttttgc cccaacggaa aatagtcggt ctgcccgtaa
       61 aatcagccgt ttgtccgggt gcagccgggg ctttgggctt cagacggcat attttcggaa
      121 tggcggcatt cttgccgtcg gcgcggcagc cgtatgggga agggagggga tattgtggtc
      181 ggtaacggca aaaaatatgc cgcaccattg ctggtgctgg gttgcgtggt gttcggtctg
      241 ggcagtctga ttgtcagatc cgtccccgtc ggttcgtatg caatcgcatt ttggcggttg
      301 ctgatttcgg tgttcgtatt ttggttttta gcacggtttt tcgggcaaaa attcccaaaa
      361 aacaggaaaa ccgtccgata tgccctgacg gcgggcgtgt ttctcgcttt cgatttggcg
      421 ttgtggcacg aaagcataca cgcggtcggg ccgggtattt ccaccctgct caacagcctg
      481 caaatctttt tcttgtcggc aatcggtgtt ttctttttcg gcgagcgttt gagcgggctg
      541 aaaaaggcag gcttaatatc ggcagttgcc ggcgtggcga tgattgccgg tgcggaattc
      601 ggctacaacg gtaatgcggt ttggggattc gccagcggtt tggtatcggg actgatgctc
      661 gccctgtcga tggtgttcgt ccgcaaaacc catgaaatcg agccggtggc gcttttccct
      721 tcaatgatga ttttgagttt gggcggcgcg gtatcgctgg ttgttccggc attgctgatg
      781 gatggcggcg tgctttatcc gacgacttgg aaagatgcgg gtttggtgct tgtgtacggc
      841 gtggtgatgc agtgcttcgc gtgggcgatg gttgcctatg cgattccgct gctttcgctg
      901 tcgctgacgg ggctgctgct tttgtccgaa ccggttgccg ccctgttcat cgattatttc
      961 gggttgggca aaccgattga aggcgtgcag tgggcagggg tggcgctgac gctttcggca
     1021 atttacctcg gttcgctgaa acagcagtct taatattgat tccatcaagg cagtattggc
     1081 agttttccgt gcaaaacctg cgttaccgga ctg
//
LOCUS       DAXHKX010000269         1067 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXHKX010000269 DAXHKX010000000
VERSION     DAXHKX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.269"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..242
                     /locus_tag="ACFMWZ_002109"
     CDS             <1..242
                     /locus_tag="ACFMWZ_002109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935994.1"
                     /translation="GETTTYRFLLIRYTTMNPSQYLFIDLNFDKIPDTRLCCFSIFQP
                     TVNLKQPVHAMLQFLFLSADCFDKELKIHFMHLKI"
     gene            350..910
                     /locus_tag="ACFMWZ_002110"
     CDS             350..910
                     /locus_tag="ACFMWZ_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HFC5935995.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          307 a          294 c          216 g          250 t
ORIGIN      
        1 aaggcgagac aacgacgtac cggtttttgt taatccgcta taccacgatg aatccttcgc
       61 aatatctgtt tatcgacctc aattttgaca aaataccgga tacgcgcctt tgttgctttt
      121 ccatcttcca accaactgta aatctcaaac agccggtaca cgccatgctt cagtttcttt
      181 tcctgtcggc ggattgtttc gacaaagaat tgaaaatcca tttcatgcac cttaaaattt
      241 aatctgcatt caaacctttt cactttggaa gcaccattta tcggatgtcc cttcgcaata
      301 aacaaatttt cccgataccg ccgcccattt caacccaaac ccaaaagcta tgaaaaacct
      361 catcgccttc aacaaaccct atggcgttat ctgccaattt tcaccgcacg aaaaacacaa
      421 aagcctcaaa gactttatca atcttcccgg cttctacccc gccggacggc tcgacaccga
      481 cagcgagggg ctgctgctgc tgaccgacga cggcaggctt caggcacaaa ttaccgaccc
      541 caaattcaaa caccccaaaa cctactgggc acaactggag ggcgtacccg acgaaagccg
      601 attggaaagc ctaagaaaag ggatagactt aggcggtttc gttacccgtc cggcaagcat
      661 ccgcatcttg aaacacggag aagcagattc gttatgggag cgcatcccgc cgatacgcgt
      721 ccgcaaaacc gttcccgatt tttggattga aattaccatt tctgagggca aaaaccgcca
      781 agtcaggcga atgaccgcca aggcgggcta tccctgcctg cgtctgatca gagtggcaag
      841 cggcaggctg aaactgtttg atttggattt aaaacccggg gaatgggcat acgccccgtt
      901 taaaccataa tcacgtttat ctcatcattt ccacaaaagt gggaatccgg aattttatag
      961 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacgaaac
     1021 cgattcactc ggtgcttcag caccttagac aatcgttctc tttgagc
//
LOCUS       DAXHKX010000270          993 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXHKX010000270 DAXHKX010000000
VERSION     DAXHKX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.270"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..200
                     /locus_tag="ACFMWZ_002111"
     CDS             <1..200
                     /locus_tag="ACFMWZ_002111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935996.1"
                     /translation="GNLGDFEEFCKGLKRMKSTDIQTQSFLAHFQRIVNAENFVNRFF
                     AQVSASFRGMGNRIRKRTHAT"
     gene            263..934
                     /locus_tag="ACFMWZ_002112"
     CDS             263..934
                     /locus_tag="ACFMWZ_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC5935997.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          284 a          217 c          225 g          267 t
ORIGIN      
        1 gtgggaattt aggggatttt gaggaatttt gcaaaggtct caaacggatg aaatcaaccg
       61 atattcaaac acagtcattt ttagcgcatt ttcagcgtat cgttaatgcg gaaaatttcg
      121 tgaacaggtt ttttgcacag gtctccgcct cgtttcgcgg gatgggaaac cgtattagaa
      181 aacggacgca cgcaacataa accccgaaag tgatgataag atgatgattt aacgtactgc
      241 tttaattatt taaggaatta tcgtgtttcc cgacaaatac aagttgagtt tggaagaaaa
      301 tatttttctg gcaaagaaag tattggttgc ccaaattcac aacctcagcc gttttgagaa
      361 ttgtcagacg accttgttgc agaccgaaca aattatccat ggcaaaaatg tagcctccgc
      421 gtcactggaa gacatccaaa ccatcttgaa cctgaaacgt gcctatcaat atgtgatttc
      481 gcatatttca aacggcgaac cggtcgatat ttcactcctt aaaaaaatca acaacattgt
      541 tgccaaggac gattctttgg cacccggtga tttccgtacc ggttcggtcg gcgtaacgct
      601 attggacggt tcccgtcatg ccccaaatcc ggtgaaggaa attgaagtgg ctcgcgtgtt
      661 acaaaatatc ggactgcaaa gcggttcgac gacggaggca gccgtccgtt ttatgcttta
      721 ttgtatgcgg cagcaggttt tttgggacgg caacaaacga acggcaacct tatttgccaa
      781 cggtctgatg atggcggggg gctgcggcat cttggaaatc tccgaaatgc agatgccgca
      841 attcaatgaa aaactgtccg cattctatcg ctccggcgac gataccgata tttccaagtt
      901 tgtgtatcaa aattgtatat cgggcataga ctgagacctt tgcaaaattc cccaaaatcc
      961 cctaaattcc caccaagcca tttaggggat ttt
//
LOCUS       DAXHKX010000271          988 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXHKX010000271 DAXHKX010000000
VERSION     DAXHKX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.271"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(17..955)
                     /locus_tag="ACFMWZ_002113"
     CDS             complement(17..955)
                     /locus_tag="ACFMWZ_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5935998.1"
                     /translation="MDAYTEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT          182 a          280 c          315 g          211 t
ORIGIN      
        1 gtcgtctgaa aacgggctaa cggctgccca gcggcaatcc gatgtccgaa atcagcctgt
       61 cgggcaggac gtcgtcgcgg aagcggcaaa gctgcgcggg acggcctttg ctgcccgata
      121 cgccggtatc cgacggctcg ataaggtttt gctgctgaat ctggcggcgg aagttttgct
      181 tgtgcagcaa tcttccgctg atggcttcga cactgttttg cagttgcagc agcgtgaatt
      241 cgggcggcat cagttcaaaa atcacggggc ggtatttgat tttggcgcgc aggcgggaca
      301 gggcggtcgc cagtacgcga cggtgatcgt ggcgcatggg ctgccccgtg agcgcgaagt
      361 cgaagtttgc ctgcggctcg gcggcttcca ctatcagacc gctttcatac agcatttcat
      421 agcgttgtaa aacgtattct tccgaccagt tttccggttc gaccccccag cacaaatgaa
      481 tgcgcttgag ccgcatttga cgcacttcct ccgtgtccgc cgaatttgcc caaatgcgca
      541 ggcggctgac gacggcgtcg cgctgcccgc cgtcggtgcg caagtcttcc cacgggaaat
      601 agccgtagca gtcctgccat ttcgcatccg ggtgcaggat gctgtcggct gcctcgcgca
      661 ccagccccaa atagctgacg tacaagacgg gcatgccgtg ttcgttgcgg cggtgggtat
      721 cgacaaaggt gtaaagctgt tccacatagc ccataggctg cgaagtctgc ttggcaaccc
      781 acagcttcac gcccgcctgc agggaattgc gcagagggga gagcgggccg ttgggcagga
      841 gtgtgccttg ggcgacggtt aatacccgca ggccgccgtc ggtaacggca atcaatacgg
      901 gaaccagctc gacgatgctt tgcggcgggg cttcggcttc ggtgtaggca tccatgctcg
      961 gctttctgcg ggtgggaaag gctttatt
//
LOCUS       DAXHKX010000272          977 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXHKX010000272 DAXHKX010000000
VERSION     DAXHKX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.272"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..>977
                     /gene="pilC"
                     /locus_tag="ACFMWZ_002114"
     CDS             <1..>977
                     /gene="pilC"
                     /locus_tag="ACFMWZ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC5935999.1"
                     /translation="AQTSKYAIIMNERNQLEVKQEGSYSTLREKDRERKFDFNAHRGG
                     GGSVFFDNTDTLVSRQSGTAVFGTATYLPPYGKVSGFDANALKERNNAVDWIHTTRAG
                     LAGYTYTNVICRSSDQCPQLVYETKFAFSNKDLANNAGRLDRHSDPSRENSPIYKLKD
                     HPWLGVSFNLGSEGTAKDGKSFNKLVSSFDENNSNQNLVYTTKGHHISLGDWQREHTA
                     MAYYLNAKLHLLDKKGIKDIAQGKTVDLGTLRPRVEAKVRRGGRNLLNFWATWKIEDK
                     GNITVRLDLPEVKAGRCTNAAHPNPKVQALSPALTAPALWFGSVQNGKA"
BASE COUNT          280 a          257 c          241 g          199 t
ORIGIN      
        1 aggcgcaaac cagtaaatac gctattatca tgaacgaacg aaatcagctg gaggtaaagc
       61 aggagggttc atattcaacc ttaagggaaa aagacaggga acgcaaattt gactttaatg
      121 cgcacagagg aggtggaggt tctgtctttt tcgacaatac cgataccctt gtttcccgac
      181 aaagcggtac tgccgttttt ggcacagcca cctacctgcc gccctacggc aaggtttccg
      241 gttttgatgc caacgctctg aaagagcgca acaatgccgt cgattggatt cataccaccc
      301 gggccgggct ggcaggctac acctacacca acgtcatatg cagaagcagc gaccaatgcc
      361 cccaacttgt ctatgagacc aaatttgcct tcagcaacaa agatttggca aacaatgcgg
      421 gcaggctgga taggcactcg gacccaagcc gcgaaaattc gcccatttac aaattgaaag
      481 atcatccatg gttgggcgta tctttcaatt tgggcagcga gggtaccgcc aaagatggca
      541 aatcattcaa caaattggta tcttcttttg atgaaaacaa tagtaatcaa aacctcgtct
      601 atacgacaaa aggccaccat atttcccttg gcgactggca gcgcgaacat accgccatgg
      661 cctattatct gaacgccaaa ctgcacctgc tggacaaaaa agggattaaa gatatcgccc
      721 aaggcaaaac agtggatttg ggcaccttga gaccgcgcgt cgaggcaaag gtaaggcggg
      781 gggggaggaa tctgctaaat ttttgggcta cgtggaagat tgaagataaa gggaacatta
      841 cagtccgcct cgacctgccg gaagtcaaag ccgggcgctg caccaacgcc gcccacccca
      901 atcccaaagt ccaagccctt tcccccgcac tgaccgcccc cgcgctgtgg ttcggatctg
      961 tgcaaaatgg taaggcg
//
LOCUS       DAXHKX010000273          934 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXHKX010000273 DAXHKX010000000
VERSION     DAXHKX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.273"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(112..744)
                     /gene="mtrR"
                     /locus_tag="ACFMWZ_002115"
     CDS             complement(112..744)
                     /gene="mtrR"
                     /locus_tag="ACFMWZ_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC5936000.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          210 a          205 c          243 g          276 t
ORIGIN      
        1 ctatgttatt gcaaaggtct caatatgtat ttttagaaga aggctttgtg tcattttgat
       61 gccgtctgaa agggtttgtt cgtttcagac ggcattgtta ccaaggcttg attatttccg
      121 gcgcaggtcg ggatggtttt ccaagttgtc catcattatc ccgatgatgc gcggggcggt
      181 tttgcccaaa tcgaaacgtt cgcaggaaga gaaccaccgc caaatcagcc cgtccaaggt
      241 tgatttgatg aagatgaccg ctgtttcctt gtccaaatcg tcagccaaat cctgattttc
      301 caccgcttcg gtcaaaacgg cggtaatttt ctcgcgccag attgcctgat gcttgcgggc
      361 aatggcgata acggcggcgt tttgttccgt gtgttcgcat tttaaaaaca ggatgttgtg
      421 gaatttgtag tagatgtcgt tgctttgcag ccgctcgaaa aagtgcagca gcgtgtggcg
      481 gaataccgcc caagaccctc cttcggcatc ttcggcatct tgcgcgatgc agttttcgat
      541 gtcgtcgcag atacgttgga acagcgcgtc gaacaagtct tccttatttt tgaaatgcca
      601 atagagcgcg ccgcgcgtta cgccggcggc ttgggcgatt tcgttgagcg aggtgcgcgc
      661 aatccctttg cggtaaaagg tttccaaggc ggcaagcatc aggtgttctt tggttttcaa
      721 ggcttcggtt ttggtttttc tcataatggc gttttcgttt cgggtcggtt tgatgagggc
      781 ggattataaa aaagactttg taaccatgca atcgtgtatg tataatgaaa cccatgaaat
      841 tgagactaca tctcaacttt gaaaacaggc ataacctgct ttgcaacccg ttcgaacatt
      901 cgggtatcaa agcaggcggt ttttatatcc tgaa
//
LOCUS       DAXHKX010000274          898 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXHKX010000274 DAXHKX010000000
VERSION     DAXHKX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.274"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(178..>898)
                     /locus_tag="ACFMWZ_002116"
     CDS             complement(178..>898)
                     /locus_tag="ACFMWZ_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC5936001.1"
                     /translation="VKELVQQSTDGSDRLYAYQSGSTLNGGLGDDYLYGADGNDLLNG
                     DAGNDSIYSGNGNDTLNGGEGNDALYGYNGNDVLNGGEGNDHLNGEDGNDTLIGGAGN
                     DYLEGGSGSDTYVFGEGFGQDTVYNYHVDKNSDTMHFKGFKAADVHFIRSGSDLVLSA
                     SEQDNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAAT
                     GGNVDANIQSVQQPLLVTPSA"
BASE COUNT          249 a          220 c          179 g          250 t
ORIGIN      
        1 atagtacggt aaggcgaggc aacgctgtac tggtttttgt gaatccacta tattgctaga
       61 agtgcaccga ttaaggttaa ttaaaatacg acccttagcc caatattaag aaaaacagca
      121 atcaaaataa acttgattgc tgtttttcag tttaagccat gaatgtaatt aggctcctta
      181 tgcagatggc gttaccaata acggctgctg tacggattgt atattggcat ccacatttcc
      241 gcctgtcgca gcagtattag aaccgaacac agacatagac tgtaccaaat tattgccagc
      301 attaatatac ttggcaaaat ctggattact gatagctgca tcatcaaaga caaatgtatc
      361 tacacgatgg ttttcaccat agaagaatcc ggaaatacgt acgttgtctt gttcagaagc
      421 gctaagcacc aaatcacttc cggaacggat aaaatgaaca tctgctgctt taaatccttt
      481 aaagtgcata gtgtcagagt ttttatccac atggtagtta tagaccgtat cctgaccgaa
      541 gccttcgccg aagacataag tatccgaacc gctgccgccc tccaagtaat cattgcctgc
      601 accgccgatc agagtgtcgt taccgtcttc gccgttcaaa tgatcattgc cttcgccacc
      661 attcagtaca tcgttaccat tatagccgta cagggcgtcg ttgccttctc ctccattgag
      721 cgtatcattg ccattgccac tgtagatact gtcgttgcct gcatcaccat tcagcaggtc
      781 attcccgtcg gcaccgtaca gatagtcatc gcccaatccg ccatttaagg tacttccgga
      841 ttggtaggca tacaatctgt ccgaaccgtc ggtggattgc tgtaccagtt ctttgaca
//
LOCUS       DAXHKX010000275          881 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXHKX010000275 DAXHKX010000000
VERSION     DAXHKX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.275"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            57..782
                     /locus_tag="ACFMWZ_002117"
     CDS             57..782
                     /locus_tag="ACFMWZ_002117"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC5936002.1"
                     /translation="MPRFYLPENLSIGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          229 a          308 c          197 g          147 t
ORIGIN      
        1 gtccgtttcc atcacgccgc tgcgacagat tataaccgtc cgaaccgcca aaaactatgc
       61 cccgattcta cctgcccgaa aacctttcca tcggacaaac cgtcgcccta cccgacaaca
      121 tcgtccgcca cctcaacgtc ctgcgcgtcc gccccaacga aaacatcacc ctcttcgacg
      181 gcaaaggcaa ggcacacgcc gcacggctga ccgttttgga aaaacgccgc gccgaagccg
      241 aaatcctgca cgaagacaca accgacaacg aatccccgct caacatcaca ctgatacaat
      301 ccatctcctc cggcgatcgc atggatttta ccctgcaaaa aagcgtcgaa ctcggcgtaa
      361 ccgccataca gcccgtcatc agcgaacgct gcatcgtccg cctcgacgga gaacgcgccg
      421 ccaaacgcct cgcacgctgg caggaaatcg tcatctccgc gtgcgaacaa agcggcagga
      481 acaccgttcc ccccgtactg cctatcatcg gctaccgtga agcactcgac aaaatgccgt
      541 ctgaaagcac caagctgatt atgagcatca accgcgcccg caaactcggc gacatccgcc
      601 acccgtccgg cgcaatcgtc tttatggtcg ggcccgaagg cggctggaca gaacaggaag
      661 aacaacaggc atttgaagct ggctttcagg cggttacact cggcaaacgg attttacgca
      721 cagaaaccgc cccactcgcc gccctcgccg ccatgcagac gctttggggc gatttcgcat
      781 aaacagaaat gccgtctgaa acccgttcag acggcatttt gcagccgatt aagatagtag
      841 gttcaaataa gatttccccc gccgtcattc ccgcgaaagc a
//
LOCUS       DAXHKX010000276          875 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXHKX010000276 DAXHKX010000000
VERSION     DAXHKX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.276"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(55..834)
                     /locus_tag="ACFMWZ_002118"
     CDS             complement(55..834)
                     /locus_tag="ACFMWZ_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC5936003.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          205 a          288 c          173 g          209 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcgaccaaa acattcaatg ccgacctgac cgacgggcgg gacacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg cagaaccctt ccgaa
//
LOCUS       DAXHKX010000277          859 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXHKX010000277 DAXHKX010000000
VERSION     DAXHKX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.277"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            409..615
                     /locus_tag="ACFMWZ_002119"
                     /pseudo
     CDS             409..615
                     /locus_tag="ACFMWZ_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            615..812
                     /locus_tag="ACFMWZ_002120"
                     /pseudo
     CDS             615..812
                     /locus_tag="ACFMWZ_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          245 a          229 c          187 g          198 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacagga aaaaactaca ccaaactaca
      121 atttttgttt atgcaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 actctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttatttgc cgtgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgcattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cactgaccca actcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggaaaat ccacacgcat cggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc accatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaacat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
      841 cagacagtac aaatagtac
//
LOCUS       DAXHKX010000278          805 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXHKX010000278 DAXHKX010000000
VERSION     DAXHKX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.278"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..743)
                     /locus_tag="ACFMWZ_002121"
     CDS             complement(join(<1..524,524..743))
                     /locus_tag="ACFMWZ_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1301 family transposase"
                     /protein_id="HFC5936004.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAK"
BASE COUNT          193 a          175 c          160 g          277 t
ORIGIN      
        1 cttagcatga ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt
       61 ctcatagcga gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa
      121 gtggcgtaat ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg
      181 ctttggtata atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc
      241 ataccctttg tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc
      301 aacttgaccg tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga
      361 gtccacacat aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc
      421 gaaaccacgc cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa
      481 tacccattct ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt
      541 ttagaccatc ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg
      601 tctcgccatg gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg
      661 ttcttccatt gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg
      721 tcagttattg cggttctcgc cattgtttgt gtttgcctat atttcgttgg aatatagggt
      781 taaaataggg ctcttttggg cttat
//
LOCUS       DAXHKX010000279          742 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXHKX010000279 DAXHKX010000000
VERSION     DAXHKX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.279"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..425
                     /locus_tag="ACFMWZ_002122"
     CDS             <1..425
                     /locus_tag="ACFMWZ_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC5936005.1"
                     /translation="EYYLNHGKWPDGNSSAGVASASDIKGKYVEKVEVKNGVVTATML
                     SSGVNNEIKGKKLSLWAKRQDGSVKWFCGQPVTRTAADNINTKHLPSTCCDASSAVCI
                     ETPRADFKHFQKISRHRVLPESRHMARRQQFCRRGNLL"
     gene            340..741
                     /locus_tag="ACFMWZ_002123"
                     /pseudo
     CDS             340..741
                     /locus_tag="ACFMWZ_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          221 a          222 c          166 g          133 t
ORIGIN      
        1 cagagtatta cctgaatcac ggcaaatggc ctgacggcaa cagttctgcc ggcgtggcat
       61 ccgcctccga cattaaaggc aaatatgttg agaaagttga agtcaaaaac ggcgtcgtta
      121 ccgccacaat gctttcaagc ggcgtaaaca acgaaatcaa aggcaaaaaa ctctccctgt
      181 gggccaagcg tcaagacggt tcggtaaaat ggttctgcgg acagccggtt acgcgcaccg
      241 ccgccgacaa catcaacacc aagcacctgc cgtcaacctg ctgcgacgct tcatctgccg
      301 tttgcataga aacaccacgc gccgacttca aacacttcca aaaaatcagc cgtcacagag
      361 tattacctga atcacggcat atggcccggc gacaacagtt ctgccggcgt ggcaacctcc
      421 tctaaaatca aaggaaaata tgttaaggaa gttacagtcg caaacggcgt cgttaccgcc
      481 acaatgcttt caagcggcgt aaacaacgaa atcaaaggca gaaaactctc cctgtgggcc
      541 aagcgtcaag acggttcggt aaaatggttc tgcggacagc cggttgagcg caccgccccc
      601 accacaacca acgacgacgt caaagccgac accgccgcca acggcaagat tgacaccaag
      661 cacctgccgt caatctgccg cgacgcttca tctgtcgttt gcatagaaac accacctacg
      721 gctttctata aaaataccta aa
//
LOCUS       DAXHKX010000280          733 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXHKX010000280 DAXHKX010000000
VERSION     DAXHKX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.280"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT          168 a          179 c          197 g          189 t
ORIGIN      
        1 agccccttag atttaattca ctatacctgc gtaatggtat cgggtactca tcatcaacct
       61 gccttgcatt cggttgtgtt gtccggcaac tgtccggttg tgtcgatggg ttgccctgcc
      121 gctccctgtg ctgcgcaacg gttgtgtgcc ttgggtgggc gcgggtggcg gccgggcggt
      181 ttgttgctat gatacgggag ttccgatata caagggtggg cttcagccca ccaatcaacg
      241 accgaaaaag aaaaaacgtt gggctgaagc ccactctacg caattacgca actgttatcg
      301 tggggatgac gggattttag gtttttgttt ttctgttttt gcgggaagga tgggttttag
      361 gttgcggtca tttatcggaa aagcagaaat cgctccgccg tcattcccgc gaaagcggga
      421 atctgaacac gtccgtaggg aaacctatat cccgtcattc ccacgaaagt gggaatctag
      481 aacctctgaa ttttcagaca acctttgaat attgccgccg tcccgcgttc tagattcccg
      541 cctgcgcggg aatgacgatt catcagtttc ccgaaacgcc cgaaaaaccg aaaccgagca
      601 aaccggattc ccgcctgcgc gggaatgacg atttataagt ttcccgaaac gcccaaaaaa
      661 accgaaatcc gacaggcggg atttttgctt tcgtggggat gacgggattt taagttacgg
      721 tcatttatcg gaa
//
LOCUS       DAXHKX010000281          728 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXHKX010000281 DAXHKX010000000
VERSION     DAXHKX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.281"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..616
                     /locus_tag="ACFMWZ_002124"
     CDS             <1..616
                     /locus_tag="ACFMWZ_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC5936006.1"
                     /translation="TGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAADYASYR
                     KWNNNKYSVNTKELENKNNNKRDLKTENQENGSFHAASSLGLSAIYDFKLNDKFKPYI
                     GARVAYGHVRHSIDSTKKTTEVLTSSHAPGTAPTIYNQGSTQNAYHESHSIRRLGLGV
                     IAGVGFDITPKLTLDTGYRYHNWGRLKNTRFKTHEVSLGMRYHF"
BASE COUNT          218 a          213 c          154 g          143 t
ORIGIN      
        1 aaccggtgca aacaacacaa gcacagtaag cgattatttc agaaacatcc gtgcgcattc
       61 catccacccc cgggtgtcgg tcggctacga tttcggcgac tggagaatag cggcagatta
      121 tgccagttac agaaaatgga acaacaataa atattccgta aacacaaaag agttggaaaa
      181 caagaataac aataagagag acctgaagac ggaaaatcag gaaaacggca gcttccacgc
      241 cgcttcttct ctcggcttat ccgccattta cgatttcaaa ctcaacgata aattcaaacc
      301 ctatatcggt gcgcgcgtcg cctacggaca cgtcagacac agcatcgatt cgaccaaaaa
      361 aacaacagag gttcttacct cctcccatgc tcctggcaca gcacctacga tttataatca
      421 gggaagtacg caaaacgcct atcacgaaag ccacagcatc cgccgcttag gtcttggtgt
      481 catcgccggt gtcggtttcg acatcacacc caagctgacc ttggacaccg gataccgcta
      541 ccacaactgg ggacgcttga aaaacacccg cttcaaaacc cacgaagtct cattgggcat
      601 gcgctaccac ttctgattcc ccgataccga tgccgtctga accttcagac ggcatttttg
      661 atgcacctgc cgttgacaaa cgcggggcgg gcgtggaaat acccaaaccg tcattcccga
      721 caacaccg
//
LOCUS       DAXHKX010000282          694 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXHKX010000282 DAXHKX010000000
VERSION     DAXHKX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.282"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(78..569)
                     /locus_tag="ACFMWZ_002125"
     CDS             complement(78..569)
                     /locus_tag="ACFMWZ_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC5936007.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          190 a          170 c          152 g          182 t
ORIGIN      
        1 gctttgcgca taatgccgtc ctgcagccga tgctctttca gttttccgta ggtcggattc
       61 tcgaatccga cattacttca atcgtatcca atagaaaagt ccgcattgcc gccaccccaa
      121 ttatgcggat aaataccctg tttgacataa cggtgaaacg tagaaaaccc ccaatcggaa
      181 atttgtccta catagccatg tttgaccgga ttgaaatgca gataatcaaa atgccaggca
      241 aaatcggcct catcgcggat agtatattcc caaaagcgtt tttgccaaag cctgagattg
      301 ccgccgatta aatattggct gtgccgcttg atttgccgcc agcgttccga ataagcagaa
      361 tcattgtccg gcagccgcca tatggtatga agatggtcgg gcatcaacac ccatgccaaa
      421 atttcaaacg gataccgttc gcgcaccgcc attaccgcct gccgcaaagc caaacgcacc
      481 gcatcatcgg tcaaaatctt ctgccgttta ttggttacaa ccgtaaaaaa gtaagtgccg
      541 ccattgcggt aaaaacgacg gtatttcata gtattatgct cggaatgatt ttgtaggtcg
      601 gattcttgaa ttcgacattt tgggcattgc tgcaatggat tgcaatgatg ggaatgttaa
      661 aggttttgtc ggatacaagt atccgaccta cgct
//
LOCUS       DAXHKX010000283          671 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXHKX010000283 DAXHKX010000000
VERSION     DAXHKX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.283"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            180..493
                     /locus_tag="ACFMWZ_002126"
                     /pseudo
     CDS             180..493
                     /locus_tag="ACFMWZ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            522..671
                     /locus_tag="ACFMWZ_002127"
                     /pseudo
     CDS             522..671
                     /locus_tag="ACFMWZ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
BASE COUNT          205 a          172 c          172 g          122 t
ORIGIN      
        1 aaatttaatc cactatattt ccatgaaaaa gcatggaacc tttacgaaaa aaataaaaca
       61 gtcaaacaaa cacagccgtt tcagctgcac cggagcagct gacgtttgag cggcatttga
      121 cttcgccgga cggtatgtta cccttgccgt tttaaccaac aagcaacgaa aggacaaata
      181 tgggcagcct gattattgaa gatttgcagg aaggcttcgg aaaagaagca gttaaaggca
      241 aagagattac cgtgcattac acaggttggc tggaagacgg caccaaattc gccgccagcc
      301 gctgaccatc acgctcggcg tcggacaagt catcaaaggc tgggacgaag gcttcggcgg
      361 aatgaaggag ggcggcaaac gcaagctgac catcccttcg gaaatgggct acggcgcaca
      421 cggcgccggc ggcgtgattc ccccgcacgc cactttgata tttgaagtcg agctgctgaa
      481 agtgtacgaa taaagctgcc cgggcaatac cgtctgacat aatggcttca agcaacgtaa
      541 acaaagaaat caaagacaaa aaactctccc tgtgggccaa gcgtcaagac ggttcggtaa
      601 aatggttctg cggactgccg gttaagcgcg acgacgccaa cgccaccaac gacgacgtta
      661 ccgccgacaa c
//
LOCUS       DAXHKX010000284          668 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXHKX010000284 DAXHKX010000000
VERSION     DAXHKX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.284"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(127..>668)
                     /locus_tag="ACFMWZ_002128"
     CDS             complement(127..>668)
                     /locus_tag="ACFMWZ_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFC5936008.1"
                     /translation="SAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKAVPAP
                     APQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAADGKVV
                     YAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQL
                     HFEVRQNGKPVNPNSYIAF"
BASE COUNT          151 a          190 c          185 g          142 t
ORIGIN      
        1 aaacacagtc aaacaaattt atatgcccca tcccttccga ataatttgaa aacacagccg
       61 ccaaaaacaa aaatgccgtc tgaaaacctt tcagacggca tttccaattt gatttcaggc
      121 agaaagtcag aacgcgatat agctgttcgg gttaaccggt ttgccgtttt gccgcacctc
      181 gaaatgaagc tgcgttctgg aagcgtcggt attgcccatc aaagcgacct gctgcccgcg
      241 tttgacctgc tggccttcgc cgaccagtaa tttttggttg tgcccgtatg cggtcaggaa
      301 ggaagaatta tgctggatga tgaccaagtt tccgtatccc ctcaaaccgg aacctgcata
      361 aaccactttg ccgtcagccg ccgccaaaac gggctgtccc gcattaccgg caatatcgac
      421 acccttgttg ttgccgccga aatcggcaac cactttacct tgcgtcggac gctgccaaac
      481 aatgccgccg accgaacgcg tgccggaagg cgaagcggca ggagattgcg gggcgggcgc
      541 gggaaccgct ttattttccg cagcgggcgc ggcaggttgc ggcgcggact gcacaggcgg
      601 ttgcgcggcg ggtttcacag gggtttgcac ggcagccggt acggcgggcc tgctttctac
      661 ggctgagg
//
LOCUS       DAXHKX010000285          668 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXHKX010000285 DAXHKX010000000
VERSION     DAXHKX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.285"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..122
                     /locus_tag="ACFMWZ_002129"
     CDS             <1..122
                     /locus_tag="ACFMWZ_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC5936009.1"
                     /translation="SFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
     gene            106..501
                     /locus_tag="ACFMWZ_002130"
     CDS             106..501
                     /locus_tag="ACFMWZ_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC5936010.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASVSRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFVKVSG"
BASE COUNT          211 a          130 c          162 g          165 t
ORIGIN      
        1 tctcattttc tgataattca gaaatggtaa ttaaagaatc cctgaaagat ggtcataaaa
       61 tatacaaatt tgaattttgc gaaattgtcg ataattgcaa ttttgatgat gtattcgttt
      121 gaagcgaatg caaatgcagt aaaaatatct gaaactgttt cagttgatac cggacaaggt
      181 gcgaaaattc ataagtttgt acctaaaaat agtaaaactt attcatctga tttaataaaa
      241 acggtagatt taacacacat ccctacgggc gcaaaagccc gaatcaacgc caaaataacc
      301 gccagcgttt cccgcgccgg cgtattggcg gggggcggca aacttgcccg cttaggcgcg
      361 aaattcagca caagggcggt tccctatgtc ggaacagccc ttttagccca tgacgtatac
      421 gaaactttca aagaagacat acaggcacga ggctaccaat acgacaccga aaccgacaaa
      481 tttgtaaagg tctcaggcta agtgcgcctg ttgccgccta aaaggtaacc cggatgcctg
      541 attatcgggt atccggggag gattaagggg gtatttgggt aaaattagga ggtgttatgg
      601 agcgaaaaca gccgaaaacc tgtgttgggg tttcggctgt cgggagggaa aggaattttg
      661 caaaggtc
//
LOCUS       DAXHKX010000286          663 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXHKX010000286 DAXHKX010000000
VERSION     DAXHKX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.286"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(1..>663)
                     /locus_tag="ACFMWZ_002131"
     CDS             complement(1..>663)
                     /locus_tag="ACFMWZ_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HFC5936011.1"
                     /translation="KYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRGINPIF
                     LKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKRNGVVY
                     TEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSNEFARG
                     KQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMKMLRNN
                     PI"
BASE COUNT          185 a          153 c          129 g          196 t
ORIGIN      
        1 ttaaattggg ttatttcgaa gcatcttcat taatatttta cctaaatctt catgccggtg
       61 gttaaaccta aactccgttt ctttgagatg cagataaaac atctctttgg aaataccatg
      121 aaatttagcc aaacgcccct tagtataact ccaaaaagat tcaataccat tgatatgttg
      181 ctttcctcga gcaaactcat tggaaccatg atggacacgg taatgcttct catagcccat
      241 atcgacaaga ccgtcatatg ctttccagcc gtcagtattg atttcactgt cagcagatat
      301 gtgaccacgt atgaccttga ttagtgaggc tttcgaagcg tcgggaacga tttccgtata
      361 gaccacgcca ttgcgtttca gtatgccaaa tacgatggtt ttacctgacg ctccgcgacc
      421 gcgcttaccc ctgatacgtc gcgcaccaaa gtaagattca tctaattcga ccacaccgga
      481 cagtggtgag ctttgttcgc acagagcagc aatcctgtgt ctgattttca ggaaaatagg
      541 attgataccg cgtacactaa tgccggtcat tttagcagta tcggaagcgg tcaaatcaag
      601 agcgaaacag cggattattt ctctaaactt ttgctctgaa attttgctga acttttgata
      661 ctt
//
LOCUS       DAXHKX010000287          653 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXHKX010000287 DAXHKX010000000
VERSION     DAXHKX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.287"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            64..>653
                     /locus_tag="ACFMWZ_002132"
     CDS             64..>653
                     /locus_tag="ACFMWZ_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC5936012.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRISLSAANIEAENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGT"
BASE COUNT          214 a          189 c          139 g          111 t
ORIGIN      
        1 ttcagacggc atttgttatg caagtaaata ttcagattcc ctatatactg cccagacgcg
       61 tgcgtgctga agacaccccc tacgcttgct gcagaacttt cgggtaaaac cggtgtgagc
      121 attagcgcac cgtatgccaa tgagaacagc cgcatcagtc tgagtgcggc aaacatcgaa
      181 gctgaaaacg gcaaaatcaa aattcaatct tacggtgacc aatattacta tgcgagacag
      241 agcgaactct atacctttga acgccgcagc tacaaaactg gcaaatggta caaccgcaaa
      301 cacattaccg aagtcaaaga acacaaaaac gccaagcccg acgcagtaaa cctcagcgca
      361 tcccaaggca tcgacatcaa atctggtggc agcatcgacg cctacgccac cgcattcgat
      421 gcccccaaag gcagcattaa catcgaagcc gggcggaaat tgacactcta tgccgtagaa
      481 gagctcaact acgacaaact agacagccaa aaaaggcgca gatttctcgg catcagctac
      541 agcaaagcac acgacaccac cacccaagtc atgaaaaccg cgctgccctc aagggtagtt
      601 gcagaatcag ccaacctcca atcgggctgg gataccaaac tgcaaggcac aca
//
LOCUS       DAXHKX010000288          653 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXHKX010000288 DAXHKX010000000
VERSION     DAXHKX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.288"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(53..538)
                     /locus_tag="ACFMWZ_002133"
     CDS             complement(53..538)
                     /locus_tag="ACFMWZ_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC5936013.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          194 a          212 c          116 g          131 t
ORIGIN      
        1 tttcaaaccg gaaaaatgcc gtctgaatct tcagacggca ttccattcat atttattcgt
       61 cttttttgtg ttcgatggcg tttcgggaac gattgccgcc gtaaggctct tcaaccgtat
      121 attccccctc gataatatcg tcatcgcggg aaaagccctc ttttctgccc gattggttca
      181 tgttgaaaaa attttccgca cctcctgcct gcaacactgc acctccctta aacggcagca
      241 gcagcaacac cgccaacacc gaggatacga atcccggact catcagacac accgccgcca
      301 ccgtataacg gataggccac aacatctgat aaacggatac cctcccgccg cttctcattg
      361 ccgcgcccgc caataaaaga ccggacagcc ccgtatgcct gagcatcagc acaccggcgg
      421 caaaacctgc cgccatcaaa aacaacgtcc agccgccgcc caaccaatcg gcaacccaca
      481 caatcgacat aatctccaaa aacagcagca ccaaaaaacc gataccgaaa aatctcattg
      541 accgtcatcc ttatatttaa gtaaacagca aaccgcccga acaggactcc aagcgacctg
      601 cctgtaaatg attacaaaac catgtgcttc aagccgaaac aatgtgaaat cac
//
LOCUS       DAXHKX010000289          643 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXHKX010000289 DAXHKX010000000
VERSION     DAXHKX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.289"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..389)
                     /locus_tag="ACFMWZ_002134"
     CDS             complement(<1..389)
                     /locus_tag="ACFMWZ_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936014.1"
                     /translation="MCSALSSYFEGNISANLVQPLNFSDGISLHDCYCTEIKVEGRNV
                     CFDFEDGFVVPDNNPNNQAGKHLKTDFSRVVLTHKNENAEDDYLFDIFEDIVFLGKRL
                     FTVRKFLDFSELLEMVNTQGYFLEFLY"
     gene            368..643
                     /locus_tag="ACFMWZ_002135"
                     /pseudo
     CDS             368..643
                     /locus_tag="ACFMWZ_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          202 a          137 c          133 g          171 t
ORIGIN      
        1 aaatacagaa actcaagaaa ataaccttgt gtattgacca tctcaagcaa ttcagaaaaa
       61 tcaagaaatt ttctgaccgt aaacaaacgt ttccctaaaa aaacgatgtc ttcaaaaata
      121 tcgaacaaat aatcatcttc ggcgttttca tttttatggg ttaaaacaac acgggaaaaa
      181 tctgttttca gatgcttgcc cgcttgattg ttcggattat tgtccggaac gacaaaaccg
      241 tcctcaaaat caaagcagac gttgcgtcct tctaccttta tctctgtgca ataacaatca
      301 tgcagagaaa taccatccga aaaatttagg ggctgtacta gattagcaga tatgttaccc
      361 tcgaaatatg aagataacgc actgcaaatt aaagaaaaaa agtacagaaa gaactgctcc
      421 gtttttttgt gctggaagtt accgcccgtt ctgccgccga tattttgggt atccatccca
      481 attcggcagc actgttctac cgtaaaatcc gcacggttat caaccatcat ttggccttgg
      541 ctgccgatga ggtttttgag ggctctgtcg agccggacga aagcgatttc ggcggacggc
      601 ataaaggtag acgtggtcgc ggtgcagcag gaaaagtggt tgt
//
LOCUS       DAXHKX010000290          640 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXHKX010000290 DAXHKX010000000
VERSION     DAXHKX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.290"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            80..>640
                     /locus_tag="ACFMWZ_002136"
     CDS             80..>640
                     /locus_tag="ACFMWZ_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC5936015.1"
                     /translation="MPMYADAGDVYCVYNFRLKQYTACQVTRVEENGSKKTYATLLAL
                     DWQGNKPLSAAELADLKPLYKDFMYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQ
                     RYASFWGDGYDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWD
                     DFENAMELKAFPCLSSLFLTKWHKNLY"
BASE COUNT          181 a          140 c          177 g          142 t
ORIGIN      
        1 atgatgtgca gatgccgtct gaagccgttt ccaagtttca gacggcatct gtctgtgaaa
       61 accgacagaa ataaaggaaa tgcctatgta tgccgatgcc ggagacgtat attgcgtcta
      121 taacttcaga ctgaagcagt acactgcctg ccaggttacc cgggttgaag aaaacggtag
      181 caaaaaaaca tatgcgaccc tgctggcttt ggactggcag ggcaacaaac cgcttagtgc
      241 ggcggagctg gcggatttga aaccgcttta caaagacttt atgtattggg aacgcggtct
      301 gcacatgtat aaggcaagtg ccgtcgtgcc gacgggatat gtacgggttg ggaataccgc
      361 gccgctggtc ggcgaagaca cgcaacggta tgcctctttt tggggcgacg gctacgacgt
      421 gtaccgtcag ttgagatggc agcagatacc cgaaaaacag agaaaggcat tcaaaaaagc
      481 cgccaaaagc aaaaagaccg tgatgtttgc cggacgggaa tacggcatat ccaaacagaa
      541 tttgagcgat gtttgggatg attttgaaaa cgcgatggaa ctgaaggcgt ttccctgcct
      601 gtcttcgctg tttctgacca aatggcataa aaatctatat
//
LOCUS       DAXHKX010000291          598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXHKX010000291 DAXHKX010000000
VERSION     DAXHKX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.291"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..298
                     /locus_tag="ACFMWZ_002137"
     CDS             <1..298
                     /locus_tag="ACFMWZ_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936016.1"
                     /translation="FLFVLVNTVKAMIENTDKSQLSELDKILDGVTSQELKLAYDFCQ
                     GKFGQAGFSYRRHPNYFYLSSLIATFPEFELSKADRDYLKGIINFDNYLLYELD"
     gene            342..>598
                     /locus_tag="ACFMWZ_002138"
     CDS             342..>598
                     /locus_tag="ACFMWZ_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HFC5936017.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT          186 a           90 c          130 g          192 t
ORIGIN      
        1 ttttcttttt gttttagtaa atacagtgaa agctatgata gaaaatactg ataaaagtca
       61 gctttcagag ttagataaaa ttttagacgg tgtcacaagt caagaattga aacttgccta
      121 tgatttttgt caagggaaat tcggtcaagc aggtttttct tatcgtaggc acccgaatta
      181 tttttatttg tccagtttaa ttgcgacttt tccagaattt gagttgtcta aagcagaccg
      241 agattacctc aaaggaatca taaattttga taactacctt ttatatgagt tagattagtc
      301 tttaatcacc tcaaaaccct atttattcaa aaggagaaaa aatgattgag ctgaaattag
      361 tagatgagag cagttttcag gcagtgctgg atttgaaaat atctgaagct gatgaacgag
      421 cacgttttgt ggctccaaat gtacgctctt tagctgacgc atggctctac cgggaaaatg
      481 aagatgtgtt tccgagggca atctattggg ataagcaagt ggttggcttt ctcctgctgg
      541 aaatagacaa ggatgaagca gaatacttta tctggcggat aatgattggt cagcagtg
//
LOCUS       DAXHKX010000292          582 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXHKX010000292 DAXHKX010000000
VERSION     DAXHKX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.292"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            46..504
                     /locus_tag="ACFMWZ_002139"
     CDS             46..504
                     /locus_tag="ACFMWZ_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936018.1"
                     /translation="MPSEHAFGRRFVGGYRSGTSEKGFQAVFGRVVTVENVIRLPEKF
                     GRFSGGLWYWAQQTQVQIRRCAVIVRMPIQPKQTSVFRKWMFDGAKVVRRMEGNLWVF
                     GMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          173 a           98 c          161 g          150 t
ORIGIN      
        1 gaaaaagacc tgcgctatat cggggaatga atcgacgcaa agaaaatgcc gtctgaacat
       61 gcgttcgggc ggcgttttgt tggggggtat cggagtggaa cgtctgaaaa agggtttcag
      121 gcggtctttg ggcgtgtggt gacagtcgaa aacgtgataa ggctacctga aaagtttggg
      181 agattttcag gtggcctttg gtattgggcg caacagacgc aggtacagat taggcggtgt
      241 gccgtaatcg tacgaatgcc gattcaacct aagcagacat cagtatttag gaagtggatg
      301 tttgatggag caaaggttgt acgaaggatg gaaggcaacc tgtgggtgtt tggtatggtc
      361 gcgcttgaaa aaacgtgttt taagggacaa atgccgtctg aaaatcggtt tcagacggca
      421 ttttctgttt atttaaagca aacaggaaaa ggcagcaata ttctgcagtc ttcctattca
      481 cacaagcgtt ttatagttaa ttaaaaacaa aatagtacaa tactcaactt tgaaggtcta
      541 accatggcat actctgcgga cttaagaaac aaagctttaa ac
//
LOCUS       DAXHKX010000293          544 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXHKX010000293 DAXHKX010000000
VERSION     DAXHKX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.293"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            57..344
                     /locus_tag="ACFMWZ_002140"
     CDS             57..344
                     /locus_tag="ACFMWZ_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936019.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLELSLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITGIMKDLFLFSCFVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          182 a           64 c           88 g          210 t
ORIGIN      
        1 atggcaaaac aggtaatgaa ttaaagacat tacctgtttt tacagaaatt tttaaaatgg
       61 atgatttgat tgcagctttt ttaggctatt tatcaggtaa atatagagaa gggaaaataa
      121 gcaggttaga actatcccta acggcaggaa gtattttctt tgcagtattc tttattcatg
      181 aactgataat ttttattgtt gatggaaata aggaaaatct ttggaatttt attactggga
      241 taatgaaaga tttattttta ttttcatgtt ttgtttgttt attattctac ttcttattat
      301 ttattgcgga aaccattgga aaaacaatca ataagattaa ataagatatt taagccgcta
      361 aaaaccattt aagtatttta tcttttctat gcggcattta tcttggaatt gaaatactgc
      421 aatcgggagg ctaaggcttc cgttgtaacg tttaccatat atttcattat tttaatttca
      481 tcaaaaaaca aaattcaaga ttatacatac cgccaatagt ttcttgattg ttttgtatag
      541 tgga
//
LOCUS       DAXHKX010000294          485 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXHKX010000294 DAXHKX010000000
VERSION     DAXHKX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.294"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..312
                     /locus_tag="ACFMWZ_002141"
     CDS             <1..312
                     /locus_tag="ACFMWZ_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC5936020.1"
                     /translation="ARVAYGHVKHQVHSVKKETTTVFTAPTQGAQKGGVIPEATISKP
                     AYHESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYR
                     F"
BASE COUNT          127 a          150 c          108 g          100 t
ORIGIN      
        1 gcgcgcgtcg cctacggaca cgttaaacat caggttcatt cagtgaaaaa agaaaccacg
       61 actgttttca ctgcaccaac gcaaggtgct caaaagggag gcgttatccc agaagctacg
      121 attagcaaac ctgcctatca cgaaagccac agcatcagca gcttgggtct tggtgtcatc
      181 gccggtgtcg gtttcgacat cacgcccaag ctgaccttgg acaccggata ccgttaccac
      241 aactggggac gcttggaaaa cacccgcttc aaaacccacg aagtctcatt gggcatgcgc
      301 taccgcttct gattccccga taccgatgcc gtctgaacct tcagacggca tttttgattt
      361 acctgccgtt tacaggcgcg gggcgggcgt ggaaataccc aaaccgtcat tcccgacaac
      421 accgtaatct tgaaacccgt cattcccgac aacaccgtaa tcttgaaacc cgccattccc
      481 gacaa
//
LOCUS       DAXHKX010000295          471 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXHKX010000295 DAXHKX010000000
VERSION     DAXHKX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.295"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..>471
                     /locus_tag="ACFMWZ_002142"
     CDS             <1..>471
                     /locus_tag="ACFMWZ_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC5936021.1"
                     /translation="KQEGLTEAGAAIIALAVTVVTSGAGTGAVLGLNGAAAAATDAAF
                     ASLASQASVSFINNKGDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALNNVSDK
                     QWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQL"
BASE COUNT          126 a          130 c          134 g           81 t
ORIGIN      
        1 aaacaggagg gcttaaccga agcaggtgcg gcgattatcg cactggccgt taccgtggtc
       61 acctcaggcg caggaaccgg agccgtattg ggattaaacg gtgcggccgc cgccgcaacc
      121 gatgcagcat tcgcctcttt ggccagccag gcttccgtat cgttcatcaa caacaaaggc
      181 gatgtcggca aaaccctgaa agagctgggc agaagcagca cggtgaaaaa tctggtggtt
      241 gccgccgcta ccgcaggcgt agccgacaaa atcggcgctt cggcactgaa caatgtcagc
      301 gataagcagt ggatcaacaa cctgaccgtc aacctagcca atgcgggcag tgccgcactg
      361 attaataccg ctgtcaacgg cggcagcctg aaagacaatc tggaagcgaa tatccttgcg
      421 gctttggtca ataccgcgca tggagaagca gccagtaaaa tcaaacagtt g
//
LOCUS       DAXHKX010000296          469 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXHKX010000296 DAXHKX010000000
VERSION     DAXHKX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.296"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(161..>469)
                     /locus_tag="ACFMWZ_002143"
     CDS             complement(161..>469)
                     /locus_tag="ACFMWZ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC5936022.1"
                     /translation="ARVAYGHVKHQVHSVETKTTIVTSKPTGGATVGGTIPSSVIKPA
                     YHERNSISSVGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF
                     "
BASE COUNT           95 a          108 c          145 g          121 t
ORIGIN      
        1 cgggtttcaa gattgcgctg ttatcgggaa tggcgggttt caagattacg gtgttgtcgg
       61 gaatgacggt ttgggtattt ccacgcccgc cccgcgtctg taaacggcag gtgaatcaaa
      121 aatgccgtct gaaggttcag acggcatcgg tgtcggggaa tcagaagcgg tagcgcatgc
      181 ccaatgagac ttcgtgggtt ttgaagcggg tgttttccaa gcgtccccag ttgtggtagc
      241 ggtatccggt gtctaaagtc agcttgggcg tgatgtcgaa accgacaccg gcgatgacac
      301 caagacccac gctgctgatg ctgtttcttt cgtgataggc aggtttgatg accgaacttg
      361 ggatagtgcc tcccactgta gcacctcccg ttggtttaga ggtaacaatc gtggttttgg
      421 tttccaccga atgaacctga tgtttaacgt gtccgtaggc gacgcgcgc
//
LOCUS       DAXHKX010000297          467 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXHKX010000297 DAXHKX010000000
VERSION     DAXHKX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.297"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            310..>467
                     /locus_tag="ACFMWZ_002144"
     CDS             310..>467
                     /locus_tag="ACFMWZ_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC5936023.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRP"
BASE COUNT          134 a           98 c          120 g          115 t
ORIGIN      
        1 atgccggtca gtgtgccgca ggcggttagg acgaggggga tgaggaggag cagggttttc
       61 atagcggggt ttgtttgatg ttgaacggat tttgagtgta aagggatttt aagggtttgt
      121 aaacgaaaga ggaaaaaatg ccgtctgagc ggcggaaatg gctttcagac ggtattttga
      181 ctgtttaaag cgggggcagt tctacaaacg gaaagaaatg ctgaaacctc tgataaattt
      241 caggatgaga cctttgcaaa attccccaaa atcccctaaa ttcccaccaa gacatttagg
      301 agattttcca tgagcacctt ctttcaacaa accgcgcaag ccatgattgc taaacacatc
      361 gaccgtttcc ctctattgaa gttggatcaa gtgattgatt ggcagccgat cgaacaatac
      421 ctgaatcgtc aaaaaacccg ttaccttcga gaccaccgcg gccgtcc
//
LOCUS       DAXHKX010000298          458 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXHKX010000298 DAXHKX010000000
VERSION     DAXHKX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.298"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            1..458
                     /locus_tag="ACFMWZ_002145"
                     /pseudo
     CDS             1..458
                     /locus_tag="ACFMWZ_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          135 a          146 c           95 g           82 t
ORIGIN      
        1 tacctgaatc gtcaaagaac ccgtttacct tagagaccac cgcggccgtc ccgcctaccc
       61 gctgctgtcc atattcaaag ccgtcctgct cggacaatgg cacagtctct ccgatcccga
      121 actcgaacac agcctcatca cccgcatcga tttcaacctg ttttgccgtt tcgacgaact
      181 gattaaccgc caactgaccg aaaaaggcct aaaaatagag aaagcatccg ccgccgttgt
      241 tgacgccacc attattcaga ccgccggcag caaacagcgt caggccatag aagtcgatga
      301 agaaggacaa atcaacggtc aaaccacgcc gagtaaggac agcgatgccc gttggatcaa
      361 gaaaaacggc ctctacaaac tcggttacaa acaacatacc cgtaccgatg cggaaggcta
      421 tatcgagaaa ctgcacatta cccccgccaa tgcccatg
//
LOCUS       DAXHKX010000299          454 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXHKX010000299 DAXHKX010000000
VERSION     DAXHKX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.299"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..>454
                     /locus_tag="ACFMWZ_002146"
     CDS             <1..>454
                     /locus_tag="ACFMWZ_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC5936024.1"
                     /translation="DEAILLAEGQKSAVTEYYLNHGIWPANNSDAGVASSASDIKGKY
                     VQSVIVANGVVTAEMKSTGVNKEIKGKELSLWAKRQNGSVKWFCGQPVTRANANGANN
                     DEVTAATGNDTDKIDTKHLPSTCRDASSAVCTKTPEYYLNHGEWPGNNT"
BASE COUNT          137 a          141 c          104 g           72 t
ORIGIN      
        1 gacgaagcca ttcttttggc tgaaggtcaa aaatcagccg tcacagagta ttacctgaat
       61 cacggcatat ggcccgccaa caacagtgat gccggcgtgg catcctccgc ctccgacatc
      121 aaaggcaaat atgttcaaag cgttatagtc gcaaacggcg tcgttaccgc cgaaatgaaa
      181 tcaaccggcg taaacaaaga aatcaaaggc aaagaactct ccctgtgggc caagcgtcaa
      241 aacggttcgg taaaatggtt ctgcggacag ccggttacgc gcgccaacgc caacggcgcc
      301 aacaacgacg aagtcaccgc cgccaccggc aacgacaccg acaaaatcga caccaagcac
      361 ctgccgtcaa cctgccgcga cgcatcatct gccgtttgca caaaaacacc cgagtattac
      421 ctgaatcacg gcgaatggcc cggcaacaac actt
//
LOCUS       DAXHKX010000300          448 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXHKX010000300 DAXHKX010000000
VERSION     DAXHKX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.300"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..>448
                     /locus_tag="ACFMWZ_002147"
     CDS             <1..>448
                     /locus_tag="ACFMWZ_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HFC5936025.1"
                     /translation="QPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHQITLHHSTIY
                     RYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQKSRIGD
                     WEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARAAVRAL"
BASE COUNT          134 a          145 c           94 g           75 t
ORIGIN      
        1 acaaccctat aagctcgatt cgcagctgat tcagcacatc gacaccctta tccgccgcaa
       61 actcagtccc gaacaagtat gcgcctacct gtgcaaacac caccagatca cgctccacca
      121 cagcaccatt taccgctacc ttcgccaaga caaaagcaac ggcagcacgt tgtggcaaca
      181 tctcagaata tgcagcaaac cctaccgcaa acgctacggc agcacatgga ccagaggcaa
      241 agtacccaac cgtgtcggca tagaaaaccg acccgctatc gtcgaccaga aatcccgtat
      301 cggcgattgg gaagccgaca ccattgtcgg caaaggacag aaaagcgcat tattgacctt
      361 ggtcgaacgc gttacccgct acaccatcat ctgcaaattg gatagcctca aagccgaaga
      421 cactgcccgg gcagctgtta gggcatta
//
LOCUS       DAXHKX010000301          409 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXHKX010000301 DAXHKX010000000
VERSION     DAXHKX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.301"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..>409)
                     /locus_tag="ACFMWZ_002148"
     CDS             complement(<1..>409)
                     /locus_tag="ACFMWZ_002148"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFC5936026.1"
                     /translation="IRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAE
                     LDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYWQQMPSEQ
                     HEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPS"
BASE COUNT           83 a           95 c          134 g           97 t
ORIGIN      
        1 tggacggcgc gtagggcgcg gtttcggtaa acaaatcgtc cgtgccgcct aaatcgccat
       61 agacttcatc ggtggaaata tggtggaaac ggaaggcttc gtgctgttca gacggcattt
      121 gttgccagta ggcgcgggct gcttcaagca gattgaatgt gccgacgata ttggtttgga
      181 taaactcgcc tgccgaaccg atagagcggt cgacatggct ttccgccgcc aagtgcatca
      241 cggcatcggg ccggtgttgt gcgaatacgc ggtcgagttc ggcgcggtcg caaatgtcca
      301 cttgctcgaa agcatagcgg ggattatcgg caatgtcggt cagcgattcg agattgcccg
      361 cgtaggtcag tttgtcgagg ttgacgacgg aatcttgggt gtttcggat
//
LOCUS       DAXHKX010000302          402 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXHKX010000302 DAXHKX010000000
VERSION     DAXHKX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.302"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            59..361
                     /locus_tag="ACFMWZ_002149"
                     /pseudo
     CDS             59..361
                     /locus_tag="ACFMWZ_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          118 a          116 c           92 g           76 t
ORIGIN      
        1 ttcttgagat tttgcgttct agattcccac tttcgtggga atgacgggat ttaatgatgc
       61 cgccggcgtg gcatcctccc cctccgacat caaaggcaaa tatgttgaga aagttgaagt
      121 cgcaaacggc gtcattaccg cccaaatggc ttcaagcaac gtaaacaacg aaatcaaagg
      181 caaaaaactc tctctgtggg ccaagcgtca agacggttcg gtaaaatggt tctgcggaca
      241 gccggttacg cgcgacgccc ccaacgccag cgccgacgcc gttaacaaag tcaccggcaa
      301 cgaaatcgac accaagcacc tgccgtcaac agcaccgaca agaaaatcga caccaaacta
      361 gagaaacctt tttacccgat aagtttccgc accgacgagg ct
//
LOCUS       DAXHKX010000303          384 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXHKX010000303 DAXHKX010000000
VERSION     DAXHKX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.303"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..138)
                     /locus_tag="ACFMWZ_002150"
     CDS             complement(<1..138)
                     /locus_tag="ACFMWZ_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC5936027.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
     gene            complement(135..>384)
                     /locus_tag="ACFMWZ_002151"
     CDS             complement(135..>384)
                     /locus_tag="ACFMWZ_002151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936028.1"
                     /translation="LFLIFCFWGMTGFEIAGIYRVKRKLCVTKIYPKSRQLFHRHSHE
                     SGNPGLSARKLIEKNGLRPLQNSPKSPKFPPRHLGDFS"
BASE COUNT          105 a           79 c           90 g          110 t
ORIGIN      
        1 aaggtaacgg gttttttgac ggttcaggta ctgttcgatc ggctgccaat caattacctg
       61 atccaacttc aatagcggga aacggtcgat gtgtttggca atcatggctt gcgcggtttg
      121 ttgaaagaag gtgctcatga gaaatcccct aaatgtcttg gtgggaattt aggggatttt
      181 ggggaatttt gcaaaggtct caaaccgttt ttctcgataa gtttccgtgc cgacagacct
      241 ggattcccac tttcgtggga atgacggtgg aaaagttgcc gtgatttcgg ataaattttc
      301 gtaacgcata atttccgttt tacccgataa atgcccgcaa tctcaaatcc cgtcattccc
      361 caaaaacaaa aaatcaaaaa caga
//
LOCUS       DAXHKX010000304          380 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXHKX010000304 DAXHKX010000000
VERSION     DAXHKX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.304"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(179..>380)
                     /locus_tag="ACFMWZ_002152"
     CDS             complement(179..>380)
                     /locus_tag="ACFMWZ_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936029.1"
                     /translation="KGKKLSLWAKRQDGSVKWFCGQPVKRADTDEVTADSGNDKIDTK
                     LKKPFYPISFRTDKAGFPPARE"
BASE COUNT           78 a           81 c           99 g          122 t
ORIGIN      
        1 agccttgtcg gtacggaaac ttatcgggaa aacggtttct tgagatttta catcctagat
       61 ccccactttc gtgggaatga cgaattttag gtttctgttt ttggttttct gtttttgagg
      121 gaatgatgaa attttaagtt ttaggaattt atcagaaaaa acagaaaccg ctccgccgtc
      181 attcccgcgc aggcgggaat ccagccttgt cggtacggaa acttatcggg taaaacggtt
      241 tctttagttt ggtgtcgatt ttgtcgttgc cgctgtcggc ggtgacttcg tcggtgtcgg
      301 cgcgcttaac cggctgtccg cagaaccatt ttaccgaacc gtcttgacgc ttggcccaca
      361 gggagagttt tttgcctttg
//
LOCUS       DAXHKX010000305          374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXHKX010000305 DAXHKX010000000
VERSION     DAXHKX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.305"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           98 a          115 c           82 g           79 t
ORIGIN      
        1 gaaattcgtc attcccgata acagcgcaat cttgaaaccc gtcattcccg acaacaccgt
       61 aatctcgaaa ttcgtcattc ccgcgcaggc gggaatccag ccccctgacg cggcgggaat
      121 ctatcggaaa tgactgaaac cccgcgttct agattcccac ttccgtggga atgacggttc
      181 agttgcgctc cgacaacacc gtaatctcaa aacccgtccg acaacaccgc aatcttgaaa
      241 ttcgtcattc ccgcacaggc gggaatccag acctgtccgc acagaaactt atcggataaa
      301 aacagttgcc caaaccccgc gttctagatt cccactttcg tgggaatgac gggatgcagg
      361 tttccgtatg gatg
//
LOCUS       DAXHKX010000306          366 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXHKX010000306 DAXHKX010000000
VERSION     DAXHKX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.306"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..>366)
                     /locus_tag="ACFMWZ_002153"
     CDS             complement(<1..>366)
                     /locus_tag="ACFMWZ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936030.1"
                     /translation="VKEYQPEVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFEALIRDADFQYIFLSYNNEGLMSVGQVREIFECFGKYNLVQTEYRRFKADKT
                     ENRNHKANSIFEYLHILEKT"
BASE COUNT          122 a           89 c           57 g           98 t
ORIGIN      
        1 gtcttttcta aaatatgcag atattcgaat atcgaatttg ccttatgatt acggttttct
       61 gtcttatctg ccttaaaacg ccggtattcc gtttgaacca aattatattt gccgaaacac
      121 tcaaaaatct cccttacctg cccgacagac atcaagcctt cgttgttgta gctgagaaaa
      181 atatattgaa agtccgcatc cctaatcaaa gcctcaaata ccggaaccac atccgatttt
      241 gagcaagtcc gcgctaaaac acatcgtaca gcgcaaaacc ggatgggaat ggcaaaatcc
      301 ggaaacaaac atttttcaaa gcatacaaac aaaaaaatac caacctccgg ttggtattcc
      361 ttaact
//
LOCUS       DAXHKX010000307          364 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXHKX010000307 DAXHKX010000000
VERSION     DAXHKX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.307"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(<1..283)
                     /locus_tag="ACFMWZ_002154"
     CDS             complement(<1..283)
                     /locus_tag="ACFMWZ_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC5936031.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPS"
BASE COUNT           97 a           92 c          108 g           67 t
ORIGIN      
        1 aagaaggaac gtatgtaccc gaaacggcag gtgcagacgg cggaacataa gcaggaggcg
       61 tataaaccgg cgcgctttgc accggcggca cataaggcgc atcgccggca ggagccgggc
      121 tgtacggcgt tgctccatag gggttgttgt aaactgccga agacggcgcg tcctgcatac
      181 ctgaattgcc tgcaatgacg ggggcaggct gctgggtagc acaaccgccc aacagtgcgg
      241 caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa cataagataa ccttcatgtt
      301 ccgatatata gcctgaatgc ggtatatcat aataaaaatg cgcgttcttc tcaagcgcaa
      361 agcc
//
LOCUS       DAXHKX010000308          360 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXHKX010000308 DAXHKX010000000
VERSION     DAXHKX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.308"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            32..>360
                     /locus_tag="ACFMWZ_002155"
     CDS             32..>360
                     /locus_tag="ACFMWZ_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC5936032.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSD"
BASE COUNT          112 a           97 c           86 g           65 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaagcgaaca ggaccggcgc gaacggttgc tgtcgctgac
      301 cgaaaaaggc aaagcccatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
//
LOCUS       DAXHKX010000309          343 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXHKX010000309 DAXHKX010000000
VERSION     DAXHKX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.309"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            84..263
                     /locus_tag="ACFMWZ_002156"
     CDS             84..263
                     /locus_tag="ACFMWZ_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936033.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
BASE COUNT           94 a           61 c           94 g           94 t
ORIGIN      
        1 aatttaattc actatatttg cccgtgcgtt tgtgataaat tcggcactgt atttgatatg
       61 attttaacaa atcgggaggg gcaatgtttt ggtatgtgat tggcttttgc gcctttgtcg
      121 tcgcgctgct gtcgctgtgg gtcaatgccg gcgcgttcgg tatgcaggaa gacgataccc
      181 cgcaatcgga ttatgaaagg cgtttgggct tgggtgcgaa gctgaaaaac aaaaatccgg
      241 atacggacga aaaacggcaa taaagacaac aaatgccgtc tgaaaggttt tcagacggca
      301 tttgctttgg agcggatata gtggattaac aaaaatcagg aca
//
LOCUS       DAXHKX010000310          333 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXHKX010000310 DAXHKX010000000
VERSION     DAXHKX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.310"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           96 a           73 c           71 g           93 t
ORIGIN      
        1 ttttttgaat ttcgggcaac gcttgaatct tcattccgcg caggcggaaa ttatcggtgc
       61 ggtacggcaa cttttttcga tatgaaaaga ccgtcattcc tgtaaaaaca aaaaatcaaa
      121 aacagaaaat tgaaattcgt cattcccgcg caggcgggaa tctaggacgt aaaatctata
      181 gtggattaac aaaaaccagt acagcgttgc cttgacttag ctcgaagaga acgattctct
      241 aaggtgctga agcaccgagt gagtcggttc cgcactatcc gtactgtctg cggcttcgtc
      301 gctttctcct gatttttgtt aatccactat aaa
//
LOCUS       DAXHKX010000311          319 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXHKX010000311 DAXHKX010000000
VERSION     DAXHKX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.311"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           77 a           61 c           78 g          103 t
ORIGIN      
        1 tcagttgcta cggttattgt caggtttcgg ttatgttgga atttcgggac acttatgaat
       61 cgtcattccc gcgcaggcgg gaatctagcc cgttcggtac ggaaacttat cggataaaac
      121 ggtttcttca gattttacgt tctagattcc cactttcgtg ggaatgacgg gattttaggt
      181 ttctgttttt ggttttctgt tttcgtggga atgatgaaat tttgagtttt aggaatttac
      241 cggaaaaaac agaaaccgct ctgccgtcat tcccgcgcag gcgggaatct agaccttaga
      301 acaacagcaa tattcaaag
//
LOCUS       DAXHKX010000312          314 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXHKX010000312 DAXHKX010000000
VERSION     DAXHKX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.312"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           76 a           92 c           92 g           54 t
ORIGIN      
        1 gtaagctttc caaccggcaa ttcggcgggc gcggaatcgg gcggtttacc gaaccccggt
       61 gttcgcggcg cgcctgccgc cgacggtatc ccgcgaagca agatttaagg gataaaatat
      121 gttccaacac acagggcggc acataaggcg ccgccctgat tcggaagggc ttgcacccct
      181 cccggacaaa gcctgatcct gccgccccga aggacggatg cccgaagggc ggggggtttg
      241 accgaaaagg aaatacgatg aataaaactt taaaaaggca ggttttccgc cataccgcgc
      301 tttatgccgc catc
//
LOCUS       DAXHKX010000313          304 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXHKX010000313 DAXHKX010000000
VERSION     DAXHKX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.313"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           73 a           82 c           81 g           68 t
ORIGIN      
        1 agacctgtcc gcacagaaac ttatcagata aaaacagttg ctcaaaccct gcgttctaga
       61 ttcccacttt cgtgggaatg acggttcagt tgcgtaggac tgagtcgtcg aaaggggcgg
      121 attcgatgga ttcgatgaaa acggtagaaa tgtgggattg atggaaatgg cgggccgaag
      181 cccactctac ggccccgccc tacaatacgc cttgcgaatc tgtcccacgc ccgcccttgt
      241 atgcctcaaa tgggttaaac tacgcacttc ccgacttccc gtttcgggca ggggtacagg
      301 tccg
//
LOCUS       DAXHKX010000314          301 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXHKX010000314 DAXHKX010000000
VERSION     DAXHKX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.314"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           89 a           63 c           86 g           63 t
ORIGIN      
        1 gaccggatgc ctgattatcg ggtatccggg gagggttaag ggggtatttg ggtaaaatta
       61 ggaggtattt ggggcgaaaa tagacgaaaa cctgtgtttg ggtttcggct gtcgggaggg
      121 aaaggaattt tgcaaagatc tcatcctgtt attttcacaa aaacagaaaa ccaaaaacag
      181 caacctgaaa ttcgtcattc ccgcgcaggc gggaatccag acccccaacg cggcaggaat
      241 ctatcggaaa taaccgaaac cggacgaacc tagattcccg ctttcgcggg aatgacggca
      301 g
//
LOCUS       DAXHKX010000315          295 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXHKX010000315 DAXHKX010000000
VERSION     DAXHKX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.315"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT          112 a           35 c           65 g           83 t
ORIGIN      
        1 gtgggaattt aggggatttt ggggaatttt gcaaaggtct cagacggcat ttttgtcaga
       61 aaggaaaacg gcaatatcca ctatacacac ggaagcagtc caaagatgtg aaaaccgtgt
      121 gtataagtta gtttgcgtat tgaaattaaa agataaaaat acgatgatta aaaaataggc
      181 ataagaaaag ataaacacag ccggtgtttg ccggtgtgtg tatcttttgt ggaaaaacat
      241 cttaatgtca tgtattttat ggaaaataat aacatgatta aaaaatagtc aatca
//
LOCUS       DAXHKX010000316          291 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXHKX010000316 DAXHKX010000000
VERSION     DAXHKX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.316"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..240
                     /locus_tag="ACFMWZ_002157"
     CDS             <1..240
                     /locus_tag="ACFMWZ_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC5936034.1"
                     /translation="IMRKACRNRPLAEAQTKRNRYLSKTRYVVEQSFGTLHRKFRYAR
                     AAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPVAA"
BASE COUNT           73 a           75 c           83 g           60 t
ORIGIN      
        1 attatgcgca aagcctgccg caaccgtccg ctggcggaag cgcaaaccaa acgtaaccga
       61 tatttgtcga agacccgtta tgtggtcgaa caaagcttcg gtacgctgca ccgtaaattc
      121 cgctatgccc gggcggccta tttcggactg attaaagtga gtgcacaaag ccacctgaag
      181 gcgatgtgtt tgaacctttt gaaagccgcc aacaggctaa gtgcgcctgt tgccgcctaa
      241 aaggcagcac ggatgcctga ttatcgggta tccggggagg attaaggggg c
//
LOCUS       DAXHKX010000317          290 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXHKX010000317 DAXHKX010000000
VERSION     DAXHKX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.317"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..>290
                     /locus_tag="ACFMWZ_002158"
     CDS             <1..>290
                     /locus_tag="ACFMWZ_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC5936035.1"
                     /translation="AGVGDQARADAKIILEGIKSSIHTETVSSSKSALWQKQAGRGSN
                     IETLQLPSFTGPVAPVLSAPGGYIVDIPKGNLKTQIETLTKQPEYAYLKQLQV"
BASE COUNT           86 a           94 c           66 g           44 t
ORIGIN      
        1 gcaggcgtag gcgaccaggc acgggccgat gccaagatta tcctcgaagg gatcaaaagc
       61 agcatccaca cagaaaccgt aagcagcagc aaatccgccc tctggcaaaa acaggcagga
      121 cgcggcagca acatcgaaac cctgcaactg ccaagtttca ccggccccgt tgcgcccgta
      181 ctctctgctc ccggcggcta catcgtcgac attccgaaag gcaatctgaa aacccaaatc
      241 gaaaccctca ccaagcagcc cgagtatgct tatttgaaac aacttcaagt
//
LOCUS       DAXHKX010000318          287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXHKX010000318 DAXHKX010000000
VERSION     DAXHKX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.318"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            1..287
                     /locus_tag="ACFMWZ_002159"
                     /pseudo
     CDS             1..287
                     /locus_tag="ACFMWZ_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          101 a           79 c           62 g           45 t
ORIGIN      
        1 aataccctgt ccgaattgct caaactgatt aaccgccaac tgaccgaaaa aggcctaaaa
       61 gtagagaaag catccgccgc cgtcgttgat gccaccatta ttcagaccgc cgacacaaac
      121 agcgtcaggc catagaagtt gacgaagaag gacaagttag cggccaaacc acaccgagta
      181 aggacaaaga tgcccgttgg atcaagaaaa acggcctcta caaactcggt tacaaacagc
      241 atacccgtac cgatgaggaa ggctatatcg agaaactgca catcacc
//
LOCUS       DAXHKX010000319          287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXHKX010000319 DAXHKX010000000
VERSION     DAXHKX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.319"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           95 a           65 c           51 g           76 t
ORIGIN      
        1 cggggggatt tctgtttttc cgataaattc ctaaaactta aaattccgtt atttccgcga
       61 gaacagaaaa ccaaaaacag aaacctaaaa tctcgtcatt cccacgcagg cgggaatcca
      121 gtctgttgag tttcagtcat ttccgataaa tcaccgcaac attaaatttc cagattcccg
      181 cctgcgcggg aatgacggtg gggggatttc tgtttttccg acaaattcct aaaacttaaa
      241 atttcatcat ttctacgaag acagaaaacc aaaaatagaa acctgaa
//
LOCUS       DAXHKX010000320          274 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXHKX010000320 DAXHKX010000000
VERSION     DAXHKX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.320"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           82 a           54 c           70 g           68 t
ORIGIN      
        1 ataggttcgt cattcccgca caggcgggaa tctagacatt caatgctaag gcaatttatt
       61 gaaaatgact gaaactcaaa aaactagatt cccactttcg tgggaatgac gtgatgcagg
      121 tttccgtacg gatggattcg tcattcccgc gcaggcggga atctagacat tcaatgctaa
      181 ggcaatttat tgaaaatgac tgaaactcaa aaaactggat tcccactttc gtgggaatga
      241 cgggatgcag gttcgtggga atgacgggat gcag
//
LOCUS       DAXHKX010000321          269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXHKX010000321 DAXHKX010000000
VERSION     DAXHKX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.321"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..264
                     /locus_tag="ACFMWZ_002160"
     CDS             <1..264
                     /locus_tag="ACFMWZ_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC5936036.1"
                     /translation="FYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQT
                     DFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT           89 a           67 c           51 g           62 t
ORIGIN      
        1 ttctaccaac acaccaaaat aaccaaagca ttgaaagcgg agacttattt ttgtcgccct
       61 taccattctt gggagaaagg gctgaatgag aacaccaacg gactcatccg gcaatacttc
      121 cccaaacaaa ccgatttccg taacatcagt gatcgggaga tacgcagggt tcaagatgag
      181 ttgaaccacc gaccaagaaa aacacttggc tacgaaacgc caagtgtttt attcttgaat
      241 ctgttccaac cactaataca ctagtgttg
//
LOCUS       DAXHKX010000322          267 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXHKX010000322 DAXHKX010000000
VERSION     DAXHKX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.322"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..185
                     /locus_tag="ACFMWZ_002161"
     CDS             <1..185
                     /locus_tag="ACFMWZ_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC5936037.1"
                     /translation="DGFVVPAKAGIRNRKPQKFIGKTETQKQRKPNGQDSRLRGNDGS
                     YITQKIETKQMGFPLA"
BASE COUNT           89 a           62 c           62 g           54 t
ORIGIN      
        1 tggatggatt cgtcgttccc gcgaaagcgg gaatccggaa ccggaaacca caaaaattta
       61 tcgggaaaac cgaaactcaa aaacaacgga aaccgaacgg acaggattcc cgcctgcgcg
      121 ggaatgacgg ctcatacatc acccaaaaaa ttgaaaccaa acagatggga ttccctcttg
      181 cgtgaggctg acagatgccg tctgaaagac tttcagacgg catagctttt tctctttgaa
      241 tttatagtgg attaacaaaa atcagga
//
LOCUS       DAXHKX010000323          266 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXHKX010000323 DAXHKX010000000
VERSION     DAXHKX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.323"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           83 a           53 c           74 g           56 t
ORIGIN      
        1 cggcatattc acaaaggcgc accagtcaga ggagggagag gaagggattg ttggaggcgg
       61 cgcagcattt agcagaaata aaaaacctta tccgacagcg acatgacgaa tttccccaaa
      121 aaaatcccac tgaaagcatt gaccgttttt ccctgtgggc gtatagttcg gttcttcgct
      181 gctgcagaag tggcggacga actgaaaagt atagcacaga atgttgggga tatcgagaga
      241 tatcttgaca ggcggaagaa atactt
//
LOCUS       DAXHKX010000324          257 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXHKX010000324 DAXHKX010000000
VERSION     DAXHKX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.324"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            complement(1..257)
                     /locus_tag="ACFMWZ_002162"
                     /pseudo
     CDS             complement(1..257)
                     /locus_tag="ACFMWZ_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT           84 a           53 c           42 g           78 t
ORIGIN      
        1 cagaccaaat tcccattaac taattgtctt aaaatctgaa tttgagattc tatttaaaat
       61 gccattggca tttctttaaa tgcagcccca aatgctcttt gggaatgccg ttaaacttac
      121 gtaaatggct aaattcacta atatcaagca catcataact acgaaaggta tccgtgtata
      181 caatgccatc aggcttaact ttctttcgga taattggcaa caatgtcgct gattgcgcat
      241 taggaacaac gacggta
//
LOCUS       DAXHKX010000325          242 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXHKX010000325 DAXHKX010000000
VERSION     DAXHKX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.325"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           68 a           54 c           59 g           61 t
ORIGIN      
        1 gaatctagtc cgcttgttcg gtaaatgaga gggcggattg cgcgcctgtc agataaacca
       61 cgtgtttaaa cgggcggcaa tgaggtacgc gcagagcctt gaagcgcagt cgatatatta
      121 ttttccacca aaacggacga ccccgcttgc cttgcaaacc tttaaaaagg aagccacccg
      181 gattaatccg agtggccgtg gaaaatcatt taccgcttga tttatttaaa atttatggta
      241 ta
//
LOCUS       DAXHKX010000326          225 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXHKX010000326 DAXHKX010000000
VERSION     DAXHKX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.326"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
     gene            <1..>225
                     /locus_tag="ACFMWZ_002163"
     CDS             <1..>225
                     /locus_tag="ACFMWZ_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC5936038.1"
                     /translation="GFGQDTVYNYDYATGRKDIIRFTDGITADMLTFTREGNHLLIKA
                     KDGSGQVTVQSYFQNDGSGAYRIDEIHFDN"
BASE COUNT           62 a           56 c           52 g           55 t
ORIGIN      
        1 aaggcttcgg tcaggatacg gtctataatt acgactacgc taccggacgc aaagacatca
       61 tccgctttac cgacggtatt acagccgata tgctgacttt tacccgagag ggcaaccatc
      121 ttcttatcaa ggcaaaagac ggcagtggac aagtgactgt tcagtcctat ttccagaacg
      181 atggctcagg tgcttaccgt atcgacgaga ttcatttcga taacg
//
LOCUS       DAXHKX010000327          223 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M13 240354
            SAMEA2073877-rid23338363.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXHKX010000327 DAXHKX010000000
VERSION     DAXHKX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2073877
            Sequence Read Archive: ERR494619
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378524.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378524.1
            Genome Coverage       :: 177x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/29/2024 14:29:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 59 of 102
            Pseudo Genes (internal stop)      :: 12 of 102
            Pseudo Genes (multiple problems)  :: 8 of 102
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2073877-rid23338363.denovo.327"
                     /strain="M13 240354"
                     /db_xref="taxon:487"
BASE COUNT           79 a           57 c           44 g           43 t
ORIGIN      
        1 ctttaatcag tcggacaaaa tgcctttatc cgatggaacc gttttccgcc cgaacggaaa
       61 taagcggttt tcccctgtaa acagataaaa ccaaccgggt attcatacac agccaaaaaa
      121 aagccgtccg aacccaaagg ggacagacgg ccaaacacat tacggggaaa acgtcttact
      181 caatgagtct gcgaaacaga cattgctaag acaactgcaa tta
//
